Miracle-dz/UniFAR-FTUs huggingface dataset:
Ovarian Cancer Cells in Ascites Form Aggregates That Display a Hybrid Epithelial-Mesenchymal Phenotype and Allows Survival and Proliferation of Metastasizing Cells

Peritoneal metastases are the leading cause of morbidity and mortality in ovarian cancer. Cancer cells float in peritoneal fluid, named ascites, together with a definitely higher number of non neo-neoplastic cells, as single cells or multicellular aggregates. The aim of this work is to uncover the features that make these aggregates the metastasizing units. Immunofluorescence revealed that aggregates are made almost exclusively of ovarian cancer cells expressing the specific nuclear PAX8 protein. The same cells expressed epithelial and mesenchymal markers, such as EPCAM and αSMA, respectively. Expression of fibronectin further supported a hybrid epithelia-mesenchymal phenotype, that is maintained when aggregates are cultivated and proliferate. Hematopoietic cells as well as macrophages are negligible in the aggregates, while abundant in the ascitic fluid confirming their prominent role in establishing an eco-system necessary for the survival of ovarian cancer cells. Using ovarian cancer cell lines, we show that cells forming 3D structures neo-expressed thoroughly fibronectin and αSMA. Functional assays showed that αSMA and fibronectin are necessary for the compaction and survival of 3D structures. Altogether these data show that metastasizing units display a hybrid phenotype that allows maintenance of the 3D structures and the plasticity necessary for implant and seeding into peritoneal lining.
Measurements of the Effects of Pressure-Gradient History on Separation-Bubble Transition

In many boundary-layer situations, particularly in turbomachinery, separation-bubble transition occurs at a local pressure gradient that differs significantly from the values further upstream. Additionally, this upstream history changes substantially from case to case, with the transitioning separation bubble occurring at streamwise positions along the blade chord varying from close vicinity of the leading edge to mid-chord. In the case of low free-stream disturbances, development of instability waves prior to separation would clearly vary as a result of these differences in the history of the boundary layer prior to separation.Measurements are presented to document the effects of pressure gradients that a laminar boundary layer experiences prior to separation on the transition process that follows in the separated region. The boundary layer development was measured on a smooth, flat plate upon which streamwise pressure gradients were imposed by a flexible, contoured wall opposite to the test plate. Only low freestream-turbulence levels were considered to isolate the effects of pressure-gradient history on the transition process. Two Reynolds numbers were considered for each pressure-gradient setting. Measured quantities consisted of velocity and turbulence intensity obtained with a single hot-wire, and of surface pressures. Observed variations in transition onset location with changes in pressure-gradient history provide the basis for further development of current transition prediction schemes.Copyright © 2001 by ASME
effect of independence, professionalism, and experience of internal auditors on fraud prevention with organizational culture as moderating variable

This study aims to examine the effect of independence, professionalism, and experience of internal auditors on fraud prevention with organizational culture as a moderating variable. The research data were collected through questionnaires, the research respondents were the internal auditors of Rural Banks in Bali. The method of determining the sample using purposive sampling with the criteria of internal auditors who have more than a year of work experience, with 140 respondents. The data analysis technique used in this research is Path Analysis (SEM-PLS). The results showed that the independence, professionalism, and experience of internal auditors had a positive effect on fraud prevention; and organizational culture strengthen the positive influence of independence, professionalism, and experience of internal auditors on fraud prevention.
A Novel RSCA Scheme for Offline Survivable SDM-EON With Advance Reservation

In this paper, we have proposed two new Routing, spectrum and core allocation (RSCA) schemes for offline survivable space division multiplexing elastic optical network (SDM-EON) supporting multi-class (mc) traffic in temporal domain. Multipath based protection scheme is applied to ensure survivability against single link failure, thus allowing traffic for each connection to be split amongst those paths. Advanced reservation (AR), being a part of multi-class traffic can withstand some delay from its time of arrival. Two schemes are designed based on the service of AR connections: all the split traffic in the multiple paths for a connection at the source node are set to start (i) at the same time strictly (mc-RSCA-S) and (ii) in a relaxed way by allowing different permissible times (mc-RSCA-R). Mixed integer linear programming (MILP) models are formulated for both the schemes. RSCA problem being an NP-hard one, those MILPs are not tractable and two heuristics with polynomial time complexity are developed. Two heuristics are based on a greedy approach and a simulated annealing (SA) based approach. Simulation studies of MILPs and the respective heuristics confirm that near optimal solutions are obtained by the proposed heuristics in identical experimental setup. It is observed that with an increase in the number of cores as well as in the percentage of AR connections in the incoming request set, spectrum utilization in the network and fragmentation of cores get reduced more by SA based approach compared to greedy approach.
In vivo effects of 1,4-dioxane on genotoxic parameters and behavioral alterations in Drosophila melanogaster

ABSTRACT 1,4-Dioxane (DXN) is used as solvent in different consumer products including cosmetics, paints, surfactants, and waxes. In addition, DXN is released as an unwanted contaminating by-product as a result of some reactions including ethoxylation of alcohols, which occurs with in personal care products. Consequently, DXN pollution was detected in drinking water and is considered as an environmental problem. At present, the genotoxicity effects attributed to DXN are controversial. The present study using an in vivo model organism Drosophila melanogaster aimed to determine the toxic/genotoxic, mutagenic/recombinogenic, oxidative damage as evidenced by ROS production, phenotypic alterations as well as behavioral and developmental alterations that are closely related to neuronal functions. Data demonstrated that nontoxic DXN concentration (0.1, 0.25, 0.5, or 1%) induced mutagenic (1%) and recombinogenic (0.1, 0.25, or 0.5%) effects in wing spot test and genotoxicity in hemocytes using comet assay. The nontoxic concentrations of DXN (0.1, 0.25, 0.5, or 1%) significantly increased oxidative stress, climbing behavior, thermal sensivity and abnormal phenotypic alterations. Our findings show that in contrast to in vitro exposure, DXN using an in vivo model Drosophila melanogaster this compound exerts toxic and genotoxic effects. Data suggest that additional studies using other in vivo models are thus warranted.
Is There a Link between Oropharyngeal Microbiome and Schizophrenia? A Narrative Review

The study aimed to examine the impact of the oropharyngeal microbiome in the pathophysiology of schizophrenia and to clarify whether there might be a bidirectional link between the oral microbiota and the brain in a context of dysbiosis-related neuroinflammation. We selected nine articles including three systemic reviews with several articles from the same research team. Different themes emerged, which we grouped into 5 distinct parts concerning the oropharyngeal phageome, the oropharyngeal microbiome, the salivary microbiome and periodontal disease potentially associated with schizophrenia, and the impact of drugs on the microbiome and schizophrenia. We pointed out the presence of phageoma in patients suffering from schizophrenia and that periodontal disease reinforces the role of inflammation in the pathophysiology of schizophrenia. Moreover, saliva could be an interesting substrate to characterize the different stages of schizophrenia. However, the few studies we have on the subject are limited in scope, and some of them are the work of a single team. At this stage of knowledge, it is difficult to conclude on the existence of a bidirectional link between the brain and the oral microbiome. Future studies on the subject will clarify these questions that for the moment remain unresolved.
Assistive Technology to Promote Participation in Sport for People with Disabilities.

Participation in sports is identified in the UN Convention on the Rights of Persons with Disabilities as a fundamental right and is facilitated by the use of specialized Assistive Products (AP). However, little is known about the role everyday AP plays in promoting sport participation. PURPOSE This study explores how the 50 priority APs on the World Health Organization's Assistive Products List can promote participation in sports. MATERIALS AND METHODS We used an online survey with AP-users, caregivers, and coaches (n=96). Subsequently, we performed a thematic analysis for qualitative responses describing the use of APs in facilitating sport participation. RESULTS Our results suggest everyday APs are required for sport participation for persons with disabilities. We present a conceptual model of AP use for sport participation. We found people with disability participate in a range of sports, contributing to community engagement. CONCLUSIONS Access to everyday APs is integral to achieving the rights of persons with disabilities for participation in sport.
Role of complete blood count, antioxidants, and total antioxidant capacity in the pathophysiology of acute coronary syndrome

Acute Coronary Syndrome (ACS) resulting from sudden rupture of arterial plaques with exposure of highly thrombogenic fatty deposits in the coronary arteries is one of the most common causes of mortality worldwide, needs further attention. In this case control study, a total of 138 patients with ACS and 134 nonACS controls were enrolled. The Red Blood Cell (RBC) count, hemoglobin, and hematocrit were significantly lower in patients. The White Blood Cell (WBC) and platelet counts, and neutrophil to lymphocyte ratio were significantly higher in patients (11.76 ± 2.49 × 106 cells/mL, 343.80 ± 113.76 × 109 cells/L, and 3.88 ± 2.24, respectively), which strongly indicated inflammatory responses. The serum and RBC lysate reduced glutathione (GSH), antioxidant vitamins C, D and alpha-tocopherol, total bilirubin, and Total Antioxidant Capacity (TAC) were significantly lower in ACS patients (3.78 ± 1.53 and 384.21 ± 184.86 μmol/L, 0.30 ± 0.22 mg/dL, 49.46 ± 47.11 ng/mL, 2.66 ± 3.67 μg/mL, 0.51 ± 0.27 mg/dL and 533.61 ± 232.47 μmol/L, respectively). Their serum GSH showed significant positive correlation with vitamin C and hemoglobin, and significant negative correlations were found between WBC count and serum GSH, and platelet count with TAC. Neutrophils and platelets play pivotal roles in mediating inflammatory responses, and significant reduction of antioxidants cause increased oxidative stress in patients with ACS, indicating that administration of antioxidant vitamins could reduce oxidative stress and inflammatory conditions.
Poor Diet, Long Sleep, and Lack of Physical Activity Are Associated with Inflammation among Non-Demented Community-Dwelling Elderly

Inflammation in elderly is associated with physical and cognitive morbidity and mortality. We aimed to explore the association of modifiable lifestyle parameters with inflammation among non-demented, community-dwelling elderly. A sub-sample of 117 patients with mild cognitive impairment (MCI, n = 63) and cognitively non-impaired controls (CNI, n = 54) were recruited from a large, population-based cohort in Crete, Greece, of 3140 elders (>60 years old). All participants underwent assessment of medical history/physical examination, extensive neuropsychiatric/neuropsychological evaluation, diet, three-day 24-h actigraphy, subjective sleep, physical activity, and measurement of IL-6 and TNFα plasma levels. Associations between inflammatory markers and diet, objective sleep duration, subjective sleep quality, and lack of physical activity were assessed using multivariate models. Regression analyses in the total group revealed significant associations between TNF-α and low vegetable consumption (p = 0.003), and marginally with objective long nighttime sleep duration (p = 0.04). In addition, IL-6 was associated with low vegetable consumption (p = 0.001) and lack of physical activity (p = 0.001). Poor diet and lack of physical activity appear to be modifiable risk factors of inflammation, whereas long sleep appears to be a marker of increased inflammatory response in elderly. Our findings may have clinical implications given the association of inflammatory response with morbidity, including cognitive decline, and mortality in elderly.
Methodology and analytical approach to investigate the impact of building temperature setpoint schedules

This paper presents a method for generating a large set of stochastically varying temperature setpoint schedules for building performance simulations. It analyses tradeoffs resulting from specific changes to those schedules in terms of three quantities: total electricity consumption per day; maximum hourly electricity consumption per day and predicted percentage dissatisfied (occupant thermal comfort). The method for generating schedules requires a single base schedule as the starting point and, using a few clearly defined parameters, transforms it into a set of schedules that can be used for modelling an existing building stock or for performing parametric studies. Temperature setpoint schedules are generated and simulated for a small office building in a cool-dry climate in three different case studies pertaining to changing temperature setpoint schedules. Tradeoffs between the three output metrics are significant and vary based on the temperature setpoint schedules and the time of the year.
Combined Therapy with Probiotic VSL#3 and Omega-3 Fatty Acids Attenuates Colonic Injury and Inflammation in Chronic DNBS-induced Colitis in Mice

Inflammatory bowel disease (IBD) is a progressive chronic inflammatory disease affecting the gastrointestinal tract with a chronic relapsing and remitting disease course. While there are a number of therapeutic strategies available to treat IBD, a definitive treatment still hasn’t been defined, leading to alternative treatment options including nutritional support. Herein, we planned to investigate the combined impact of probiotics and omega-3 (ù-3) fatty acids on inflammatory response and intestinal epithelium in chronic colitis induced by 2,4-Dinitrobenzenesulfonic acid (DNBS). Fifty BALB/c mice were randomly divided into five groups: non-colitis control, colitis with no treatment applied (control colitis), colitis treated with probiotics (VSL#3), colitis treated with ù-3, and colitis treated with both VSL#3 and ù-3. Colitis was induced by intrarectal administration of 2,4-Dinitrobenzenesulfonic acid (DNBS) in all groups but the non-colitis control group. Ten days after the DNBS period, phosphate-buffered saline (for both the non-colitis control and colitis control groups), VSL#3, ù-3, or VSL#3 + ù-3 treatments were administered intragastrically to their respective groups for 10 days. By analyzing the colonic expression of interleukin IL-6, IL-10, and IL-17A, and interferon gamma (IFN-ã) and tumor necrosis factor alpha (TNF-á) levels, results revealed that levels of IFN-ã, IL-17, and TNF-á were significantly higher in the control colitis group when compared with the other groups. The control colitis, colitis +VSL#3, and colitis + ù-3 groups exhibited higher scores of microscopic damage compared to the non-colitis control and colitis + VSL#3 + ù-3 groups. The closest histological image to the non-colitis control group was presented in the colitis + VSL#3 + ù-3 group. The findings indicate that the combined effect of probiotics and ù-3 fatty acids might have a protective effect against colon injury and inflammation by creating synergistic effects. However, more research is needed to understand the exact mechanism of this synergistic effect and to examine how this therapeutic approach can be used in inflammatory bowel diseases.
Test pitting medieval settlements: Big Dig 2003

In June 2004 Channel4's Time Team broadcast nine days of live archaeological investigations on more than 1,300 sites across the United Kingdom. This broke a number of records, including the number of siles investigated and the number of people involved, and as such was called 'Big Dig'. One of the guiding principles of Big Dig was that it should allow members of the general public the chance to excavate in their 'own' patch - for most this mean! digging in their gardens, while others had the chance to dig in their local park or school grounds. It is therefore not surprising to nOle that most of the sites investigated were with in settlements which were in existence in the Middle Ages, and many of them were medieval rural settlements. Publication here of details of the 1,300 Big Dig test pits is not possible, for reasons of both space and the rights to privacy of those involved. Details of the locations oflhe test pits in each county are however available on the Channel 4 Website (www.channeI4.com). and lists of the test pits are held by county archaeological officers to allow results to be accessed. However, a few general poims can usefully be made.
Experiences of Symptoms and Impact on Daily Life and Health in Hepatocellular Carcinoma Patients

Background The incidence of hepatocellular cancer (HCC) has continually increased. To achieve optimal supportive cancer care for HCC patients, it is important to consider patients’ experiences and preferences. Objective This meta-synthesis aims to critically interpret how patients with HCC experience symptoms and the impact of the disease on daily life and health. Methods Searches were performed in the following bibliographic databases: PubMed, CINAHL, Web of Science, Scopus, PsycINFO, and Cochrane Library. In addition, searches were performed using Open Gray to identify relevant studies in the gray literature. The search was limited to studies published in English from 2009 to 2019. Five studies (124 participants) were identified, appraised, and ultimately interpreted and synthesized. Results Receiving an HCC diagnosis was overwhelming and affected the patients’ entire lives. Three themes were identified based on the meta-synthesis: (1) disrupted life, (2) living with uncertainty, and (3) a changed body. Patients with HCC experience disrupted lives because of the cancer’s effect on health and multidimensional symptoms. Conclusion Available research on the experiences of HCC patients is limited. This meta-synthesis of available studies shows that being given a diagnosis of HCC is an overwhelming event. Our study findings show that an HCC diagnosis affected the individual’s entire life. Implications for Practice It is important to identify the patients’ physical, psychological, social, and existential needs during the investigation of their condition, during any curative treatment, and at the palliative stage of the disease.
Aerobic Vaginitis Diagnosis Criteria Combining Gram Stain with Clinical Features: An Establishment and Prospective Validation Study

Wet-mount microscopy aerobic vaginitis (AV) diagnostic criteria need phase-contrast microscopy and keen microscopists, and the preservation of saline smears is less common in clinical practice. This research work developed new AV diagnostic criteria that combine Gram stain with clinical features. We enrolled 325 AV patients and 325 controls as a study population to develop new AV diagnostic criteria. Then, an independent group, which included 500 women, was used as a validation population. AV-related microscopic findings on Gram-stained and wet-mount smears from the same participants were compared. The accuracy of bacterial indicators from the two methods was verified by bacterial 16S rRNA V4 sequencing (n = 240). Logistic regression was used to analyse AV-related clinical features. The screened clinical features were combined with Gram-stain microscopic indicators to establish new AV diagnostic criteria. There were no significant differences in the leukocyte counts or the parabasal epitheliocytes (PBC) proportion between the Gram-stain and wet-mount methods (400×). Gram stain (1000×) satisfied the ability to identify bacteria as verified by 16S rRNA sequencing but failed to identify toxic leukocytes. The new criteria included: Lactobacillary grades (LBG) and background flora (Gram stain, 1000×), leukocytes count and PBC proportion (Gram stain, 400×), and clinical features (vaginal pH > 4.5, vagina hyperemia, and yellow discharge). These criteria satisfied the accuracy and reliability for AV diagnosis (Se = 86.79%, Sp = 95.97%, and Kendall’s W value = 0.899) in perspective validation. In summary, we proposed an alternative and valuable AV diagnostic criteria based on the Gram stain, which can make it possible to diagnose common vaginitis like AV, BV, VVC, and mixed infections on the same smear and can be available for artificial intelligence diagnosis in the future.
Development of a multiplex TaqMan qPCR targeting unique genomic regions for the specific and sensitive detection of Pectobacterium species and P. parmentieri

The newly defined species Pectobacterium parmentieri has emerged as an aggressive pathogen that causes soft rot and blackleg diseases on potato and has been widely disseminated across the globe, jeopardizing the productivity and potato food safety. The implementation of a fast and accurate detection tool is imperative to control, monitor and prevent further spread of these pathogens. The objective of this work was to develop a specific and sensitive multiplex TaqMan qPCR to detect P. parmentieri and distinguish it from all known Pectobacterium species. A universal internal control was included to enhance the reliability of the assay.
Out of the Lab and Into the World: Analyses of Social Roles and Gender in Profiles of Scientists in The New York Times and The Scientist

Although representations of female scientists in the media have increased over time, stereotypical portrayals of science persist. In-depth, contemporary profiles of scientists’ roles have an opportunity to reflect or to challenge stereotypes of science and of gender. We employed content and linguistic analyses to examine whether publicly available profiles of scientists from New York Times and The Scientist Magazine support or challenge pervasive beliefs about science. Consistent with broader stereotypes of STEM fields, these portrayals focused more on agency than communality. However, profiles also challenged stereotypes through integrating communality, purpose, and growth. This analysis also found similar presence of communal and agentic constructs for both female and male scientists. The current findings highlight the importance of considering counterstereotypic representations of science in the media: Communicating messages to the public that challenge existing beliefs about the culture of science may be one path toward disrupting stereotypes that dissuade talented individuals from choosing science pathways.