Miracle-dz/UniFAR-FTUs huggingface dataset:
Using the Emanuel Framework to Explore the Ethical Issues Raised in a Participatory Visual Research Project in Rural South Africa

Participatory visual research can offer critical insight into the experiences of those most affected by health issues. As these methods are increasingly used to research sensitive topics, there is a need for a clear ethical framework to guide best practice on the part of researchers and research ethics committees. Here we reflect on a project where we used digital storytelling as a participatory visual methodology to explore HIV treatment adherence in rural South Africa, with a focus on the ethical issues we encountered during the lifetime of the project. To ground our reflections, we use the framework for ethical research developed by Emanuel et al., and the adaptation of this framework for social science proposed by Wassenaar and Mamotte. We suggest that fellow PVM practitioners and REC members draw on this holistic framework to support the optimal application of PVM in health research.
A Comparison of Pulsatile Hypothermic and Normothermic Ex Vivo Machine Perfusion in a Porcine Kidney Model

Background. Hypothermic machine perfusion (HMP) is a well-established method for deceased donor kidney preservation. Normothermic machine perfusion (NMP) might offer similar or greater advantages. We compared the 2 methods in an ex vivo perfusion model using 34 porcine kidneys. Methods. Thirty kidneys were stored on ice for 24 h before undergoing 4 h of HMP (n = 15) or NMP (n = 15) followed by 2 h of normothermic ex vivo reperfusion with whole blood. Four kidneys underwent 28 h of cold static storage followed by 2 h of normothermic ex vivo reperfusion. During the 2 h of normothermic ex vivo reperfusion, perfusate flow rates, urinary output, and oxygen consumption rates were compared between all groups. Results. Porcine kidneys after HMP showed significantly higher urinary output (5.31 ± 2.06 versus 2.44 ± 1.19 mL/min; P = 0.002), oxygen consumption (22.71 ± 6.27 versus 11.83 ± 1.29 mL/min; P = 0.0016), and perfusate flow rates (46.24 ± 12.49 versus 26.16 ± 4.57 mL/min; P = 0.0051) than kidneys after NMP. TUNEL staining of tissue sections showed significantly higher rates of apoptosis in kidneys after NMP (P = 0.027). Conclusions. In our study, the direct comparison of HMP and NMP kidney perfusion in a translational model demonstrated superiority of HMP; however, further in vivo studies would be needed to validate those results.
Next‐generation osteometric sorting: Using 3D shape, elliptical Fourier analysis, and Hausdorff distance to optimize osteological pair‐matching

Determining which bilateral bones belong to the same person based on shape and size similarity is called pair‐matching and it is instrumental for sorting commingled skeletons. To date, pair‐matching has popularly been accomplished by visual inspection and/or linear caliper measurements; however, attention is turning increasingly to computational analysis. In this paper, we investigate a fast three‐dimensional (3D) computerized shape‐analysis method for whole‐bone pair‐matching using a test sample of 14 individuals (23 femora, 26 humeri, and 26 tibiae). Specifically, the method aims to find bilateral pairs using, as the shape signature criterion, a single 3D outline that snakes around each bone's perimeter as described by a 3D elliptical Fourier analysis function. This permits substantial 3D‐point‐cloud data reduction, that is, to 0.02% of the starting c.500,000 point cloud or just 100 points, while preserving key 3D shape information. The mean Hausdorff distance (Hd) was applied to measure the distance between each mirrored right‐side outline to every left‐side outline in pairwise fashion (132, 168 and 169 comparisons, respectively). Both thresholds and lowest Hd were investigated as pair‐match criteria, with the lowest Hd producing the best performance results for searches jointly utilizing right‐left and left‐right directions for comparison: true positive rates of 1.00 (10/10), 1.00 (12/12), and 0.92 (11/12) for the femora, humeri, and tibiae, respectively. The computational time to calculate 469 pairwise 3D comparisons on a single stock‐standard Intel® Core™ i7‐4650U CPU @ 1.70 GHz was 5 s. This short data processing time makes the method viable for real‐world application.
Nanopore sequencing as a rapid tool for identification and pathotyping of avian influenza A viruses

We report whole-genome sequencing of influenza A virus (IAV) with 100% diagnostic sensitivity and results available in <24–48 h using amplicon-based nanopore sequencing technology (MinION) on clinical material from wild waterfowl (n = 19), commercial poultry (n = 4), and swine (n = 3). All 8 gene segments of IAV including those from 14 of the 18 recognized hemagglutinin subtypes and 9 of the 11 neuraminidase subtypes were amplified in their entirety at >500× coverage from each of 16 reference virus isolates evaluated. Subgenomic viral sequences obtained in 3 cases using Sanger sequencing as the reference standard were identical to those obtained when sequenced using the MinION approach. An inter-laboratory comparison demonstrated reproducibility when comparing 2 independent laboratories at ≥99.8% across the entirety of the IAV genomes sequenced.
Bullying and Cyberbullying among Italian Adolescents: The Influence of Psychosocial Factors on Violent Behaviours

Background: The study of adolescents’ behaviours and attitudes is crucial to define interventions for the containment of deviance and social discomfort. New ways of social interaction are crystallising violent behaviours which are moving more than ever on a virtual sphere. Bullying and cyberbullying share a common behavioural matrix that has been outlined through specific environmental and individual characteristics. Methods: A survey carried out in Italy in 2019 on a statistical sample of 3273 students highlighted the influence of several social and individual variables on deviant phenomena. Risk and protective factors in relation to the probability of involvement in bullying and cyberbullying have been shown through a bivariate analysis and a binary logistic regression model. Results: The study shows that presence of stereotypes and social prejudices, tolerance to violence and high levels of self-esteem have resulted as the main risk factors. On the other hand, low levels of tolerance related to the consumption of alcohol and drugs, high levels of trust towards family and friends and being female have been identified as protective factors. Conclusions: This research confirms the validity of several theories on bullying and cyberbullying phenomena. Furthermore, it identifies specific risk and protective factors and their influence on deviant behaviours, with a focus on environmental characteristics which appear as the key field of work to enhance adolescents’ well-being.
Effects of feed form and particle size on growth performance, nutrient digestibility, carcass characteristics, and gastric health in growing-finishing pigs

Objective This study was conducted to evaluate the effects of feed processing and particle size on growth performance, nutrient digestibility, carcass characteristics, and gastric health in growing-finishing pigs. Methods A total of 360 growing pigs (22.64±0.014 kg initial body weight [BW]) were allocated to 1 of 6 treatments with 6 replicates by BW and sex, and 10 pigs were housed in one pen in a randomized complete block design. The BW and feed intake were recorded to calculate growth performance. For the digestibility trial, a total of 24 barrows with an initial BW of 33.65±0.372 kg were split into 6 treatments with a completely randomized design. Dietary treatments were designed by a 2×3 factorial arrangement of treatments based on two main factors, particle size (600, 750, 900 μm) and feed form (mash and pellet) of diet. Experimental diets were formulated to contain the requirements of the NRC (2012). Results The BW and average daily gain were not changed by dietary treatments, and the feed intake of finishing pigs (wks 6 to 12) was increased when the pigs were fed a mash diet (p<0.05). For the overall period, the feed efficiency of pigs was improved with the pellet diet (p<0.01) and reduced particle size (p<0.05). The pellet diet had effects on increasing crude fat digestibility (p<0.01) relative to a mash diet, but there was no considerable change in dry matter and crude protein digestibilities by dietary treatments. In the evaluation of gastric health, a trend for an increased incidence of keratinization in the esophageal region was observed as particle size decreased (p = 0.07). Conclusion Feed efficiency could be improved by pellet diet and reduced particle size. Nutrient digestibility, carcass characteristics, and gastric health were not affected by feed form, and particle size ranged from 600 to 900 μm.
Novel Method for the Fast Separation and Purification of Molybdenum(VI) from Fission Products of Uranium with Aminofunctionalized Mesoporous Molecular Sieves (AMMS) Modified by Dicyclohexyl‐18‐Crown‐6 and S‐N Tetradentate Schiff's Base

Abstract A novel method for the fast separation and purification of Mo(VI) from fission products of uranium is presented. All impurities of Mo(VI) including, lead, ytterbium, zirconium, lanthanum, cerium, ytterium, uranium, strontium, and barium ions, were fast extracted by using aminofunctionalized mesoporos molecular sieve (AMMS) modified by dicyclohexyl‐18‐crown‐6 (DCCE) and 2‐({(E)‐1,2‐diphenyl‐2[(2‐2‐sulfanylphenyl)imino]ethylidene}amino)‐1‐benzenethiol (SNSB). The time of extraction, pH, and flow rate, amount of modified aminofunctionalized mesoporos molecular sieve (MAMMS), break‐through volume, and maximum capacity of MAMMS were investigated. The proposed method was applied to separation and purification of Mo(VI) from the impurities of fission products of uranium.
Do carers of adolescents at first episode psychosis have distinctive psychological needs? A pilot exploration

Background: Carers of patients experiencing first episode psychosis (FEP) are at an increased risk of mental and physical health problems themselves. However, little is known about how the psychological needs of carers may differ between those caring for an adolescent versus an adult who has FEP. Aims: This pilot study aimed to explore any differences in the psychological needs of carers caring for adolescents versus adults with FEP. Methods: We surveyed 254 carers of 198 FEP patients (34 carers of adolescents of 24 FEP adolescent patients). Carers completed self-report measures of anxiety, depression, burnout, subjective burden, coping, and key illness beliefs. The sample was divided according to whether the patient was under (adolescent) or over (adult) age 18, and analysed using mixed model logistic regressions. Results: Compared to the carers of adult patients, carers of adolescents were more than twice as likely (12% vs. 30%) to experience overall burnout syndrome (all three domains), and to develop it much quicker (19.4 vs. 10.1 months). They were also more likely to adopt behavioural disengagement avoidance as a form of coping. However, there was no difference between carers in terms of anxiety, depression, beliefs and subjective burden. For carers of adolescents, burnout was independently predicted by: a negative belief about the consequences of psychosis for the adolescent patient and an incoherent understanding of the patient’s mental health. Conclusions: If our findings can be replicated in a larger sample, then Rapid-Onset-Burnout-Syndrome (ROBS) is a particular problem in carers of adolescents at FEP, suggesting a need for routine screening and possible prophylactic intervention. Carers of adolescent’s use of behavioural escape coping maybe also require early intervention. Theoretically, consideration could be given to the development of an adolescent sub-branch to the cognitive model of caregiving.
Population‐Based Signals of Antidepressant Drug Interactions Associated With Unintentional Traumatic Injury

Antidepressants are very widely used and associated with traumatic injury, yet little is known about their potential for harmful drug interactions. We aimed to identify potential drug interaction signals by assessing concomitant medications (precipitant drugs) taken with individual antidepressants (object drugs) that were associated with unintentional traumatic injury. We conducted pharmacoepidemiologic screening of 2000–2015 Optum Clinformatics data, identifying drug interaction signals by performing self‐controlled case series studies for antidepressant + precipitant pairs and injury. We included persons aged 16–90 years codispensed an antidepressant and ≥ 1 precipitant drug(s), with an injury during antidepressant therapy. We classified antidepressant person‐days as either precipitant‐exposed or precipitant‐unexposed. The outcome was an emergency department or inpatient discharge diagnosis for unintentional traumatic injury. We used conditional Poisson regression to calculate confounder adjusted rate ratios (RRs) and accounted for multiple estimation via semi‐Bayes shrinkage. We identified 330,884 new users of antidepressants who experienced an injury. Among such persons, we studied concomitant use of 7,953 antidepressant + precipitant pairs. Two hundred fifty‐six (3.2%) pairs were positively associated with injury and deemed potential drug interaction signals; 22 of these signals had adjusted RRs > 2.00. Adjusted RRs ranged from 1.06 (95% confidence interval: 1.00–1.12, P = 0.04) for citalopram + gabapentin to 3.06 (1.42–6.60) for nefazodone + levonorgestrel. Sixty‐five (25.4%) signals are currently reported in a seminal drug interaction knowledgebase. We identified numerous new population‐based signals of antidepressant drug interactions associated with unintentional traumatic injury. Future studies, intended to test hypotheses, should confirm or refute these potential interactions.
Building a House of Skills—A Study of Functional Health Literacy and Numeracy among Patients with Type 2 Diabetes in Hungary

The purpose of this study is to explore functional health literacy (FHL) and numeracy skills in an insulin-treated, type 2 diabetes mellitus (T2DM) patient population, and their impact on diabetes self-care activities. A non-experimental, cross-sectional quantitative design was used for this study. The sample consisted of 102 T2DM patients on insulin therapy, including 42 males and 60 females, with a mean age of 64.75 years (SD = 9.180) and an average diabetes duration of 10.76 years (SD = 6.702). Independent variables were sociodemographic variables (e.g., age, educational level, etc.) and diabetes and health-related factors (e.g., duration of diabetes (years), the frequency of blood glucose testing/day, etc.). For this study, the participants completed the reading comprehension exercise from the Short Test of Functional Health Literacy (S-TOFHLA) and the Shortened Version of the Diabetes Numeracy Test (DNT-15), which specifically evaluates the numeracy skills of patients living with diabetes. The associations between the variables were examined with Spearman’s rank correlation. Multivariate regression analysis was performed to examine whether measured FHL skills impact diabetes self-care activities. We found that DNT-15 test (β = 0.174, t(96) = 2.412, p < 0.018) had significant effect on the frequency of blood glucose testing/day. Moreover, the problem areas for patients with T2DM mostly included multi-step calculations according to food label interpretations, and adequate insulin dosage based on current blood glucose levels and carbohydrate intake. The results of regression analyses and Spearman’s rank correlation indicated that limited FHL and diabetes numeracy skills not only influenced the participants’ behaviors related to self-management, but they also affected their health outcomes. Thus, besides the personalization of insulin treatment, it is indispensable to provide more precise information on different types of insulin administration and more refined educational materials based on medical nutrition therapy.
Portraying the Countercurrent Flow on Packings by Three-Dimensional Computational Fluid Dynamics Simulations

The design of packed columns requires the detailed description of the hydrodynamics on the surface of the packings. To analyze the local flow behavior of the liquid phase, a three-dimensional Computational Fluid Dynamics (CFD) model was developed that applies to the two-phase countercurrent flow on an inclined and flat plate. This model, based on the volume-of-fluid (VOF) method, considers the gravity, the surface tension and the drag force between the two phases. The development of such a model allows investigation of the influences of the liquid and gas flow rates on the flow behavior such as the film flow and the rivulet flow. A validation of the model was performed using data from the literature and from experiments conducted in this work. Simulation and experimental results demonstrate that the specific wetted area on the plate decreases with decreasing liquid load. Moreover, CFD simulations reveal that the presence of the countercurrent gas phase tends to increase the fluctuation and the thickness of the film flow, which is in accordance with experimental data. It also affects the flow behavior of the rivulet flow and changes the velocity profiles for both film and rivulet flow behavior. On the other hand, the simulation results indicate that CFD is a potent tool for analyzing and investigating the flow phenomena in chemical engineering.
Behavior assessment model for trace organics in soil. IV: Review of experimental evidence

Experimental evidence supporting the environmental screening model of Jury et al. (1983) is reviewed and discussed. For several laboratory studies of volatilization, initial and boundary conditions matched those used in our model and simulations were run. In all cases, good agreement was found between predicted and measured volatilization losses, with and without accompanying water evaporation. When five chemicals of widely differing volatility were exposed to identical experimental conditions, the model correctly predicted the relative loss behavior observed. The convective mobility predictions of the model were shown to be consistent with several laboratory studies of compound leaching, as long as the water flow rate was slow enough (< 0.01 cm/s) to ensure equilibrium between the solution and adsorbed phases. The Millington and Quirk tortuosity model used in our representation of the soil diffusion coefficient was found to give a good prediction of the water content dependence of the effective diffusion coefficient observed in several studies. The thickness of the stagnant boundary layer predicted from our similarity assumption was shown to be consistent with the apparent thickness inferred from several laboratory and field measurements of volatilization.
Targeting Myeloid-Derived Suppressor Cells in Ovarian Cancer

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that exhibit immunosuppressive activity. They also directly stimulate tumor cell proliferation, metastasis, and angiogenesis. In ovarian cancer, there are increased numbers of circulating or tumor-infiltrating MDSCs, and increased frequencies of MDSCs are associated with a poor prognosis or an advanced clinical stage. Moreover, in murine models of ovarian cancer, MDSC depletion has shown significant growth-inhibitory effects and enhanced the therapeutic efficacy of existing anticancer therapies. In this review, we summarize the current knowledge on MDSC biology, clinical significance of MDSC, and potential MDSC-targeting strategies in ovarian cancer.
Acid-Sensing Ion Channels: Focus on Physiological and Some Pathological Roles in the Brain

Acid-sensing ion channels (ASICs) are Na+-permeable ion channels activated by protons and predominantly expressed in the nervous system. ASICs act as pH sensors leading to neuronal excitation. At least eight different ASIC subunits (including ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3, ASIC4, ASIC5) are encoded by five genes (ASIC1-ASIC5). Functional ASICs assembled in the plasma membrane are homo- or heteromeric trimers. ASIC1a-containing trimers are of particular interest as, in addition to sodium ions, they also conduct calcium ions and thus can trigger or regulate multiple cellular processes. ASICs are widely but differentially expressed in the central and peripheral nervous systems. In the mammalian brain, a majority of neurons express at least one ASIC subunit. Several recent reviews have summarized findings of the role of ASICs in the peripheral nervous system, particularly in nociception and proprioception, and the structure-function relationship of ASICs. However, there is little coverage on recent findings regarding the role of ASICs in the brain. Here we review and discuss evidence regarding the roles of ASICs: (i) as postsynaptic receptors activated by protons co-released with glutamate at glutamatergic synapses; (ii) as modulators of synaptic transmission at glutamatergic synapses and GABAergic synapses; (iii) in synaptic plasticity, memory and learning; (iv) in some pathologies such as epilepsy, mood disorders and Alzheimer's disease.
Non-physiological Aortic Flow and Aortopathy in Adult Patients with Transposition of the Great Arteries after the Jatene Procedure: A Pilot Study Using Echo Planar 4D Flow MRI

Abstract Purpose Dilated aortic root and ascending aorta (AAO) with progressive aortic regurgitation is a well-known sequela after arterial switch operation (ASO) in adults with transposition of the great arteries (TGA). We aimed to quantitatively assess aortic flow profiles in adults with TGA after ASO (Jatene procedure with LeCompte maneuver) using echo planar imaging (EPI) 4D flow MRI. Methods Prospectively, 9 consecutive adults (30.2 ± 6.6 years) after ASO (Jatene operation with LeCompte technique), 13 consecutive adults (34.3 ± 7.2 years) after the atrial switch operation with Senning procedure, and 8 age-matched control patients, who underwent turbo field echo (TFE) EPI 4D flow MRI (average scan time of approximately 4 min), were enrolled. Results TGA after ASO showed a markedly dilated sinus of Valsalva, compared to TGA after atrial switch operation (26.6. ± 4.9 vs. 18.6. ± 1.5 mm/cm2). Vorticity, helicity, wall share stress (WSS), and energy loss (EL) in the aortic root and the AAO in TGA were greater than in the controls. Vorticity, helicity, WSS, and EL in the aortic root and the AAO were also greater in TGA after ASO than after atrial switch operation. More acute aortic arch angle correlated with greater vorticity of the aortic root, and the significant diameter ratio of the sinus of Valsalva and the AAO was relevant to greater vorticity, helicity, and EL in TGA after ASO. Conclusion A non-physiological blood flow pattern of the aortic root was identified in TGA adults after the ASO (Jatene procedure with LeCompte maneuver). Missing spiral looping of the great arteries and the unique structure after the Jatene procedure may play an adjunctive role in promoting aortopathy. The evaluation of aortic flow profile using EPI 4D flow MRI may be useful for risk stratification for aortopathy in this population.