Miracle-dz/UniFAR-FTUs huggingface dataset:
Recursive Feedback between Matrix Dissipation and Chemo-Mechanical Signaling Drives Oscillatory Growth of Cancer Cell Invadopodia

Most extracellular matrices (ECMs) are known to be dissipative, exhibiting viscoelastic and often plastic behaviors. However, the influence of dissipation, in particular mechanical plasticity in 3D confining microenvironments, on cell motility is not clear. In this study, we develop a chemo-mechanical model for dynamics of invadopodia, the protrusive structures that cancer cells use to facilitate invasion, by considering myosin recruitment, actin polymerization, matrix deformation, and mechano-sensitive signaling pathways. We demonstrate that matrix dissipation facilitates invadopodia growth by softening ECMs over repeated cycles, during which plastic deformation accumulates via cyclic ratcheting. Our model reveals that distinct protrusion patterns, oscillatory or monotonic, emerge from the interplay of timescales for polymerization-associated extension and myosin recruitment dynamics. Our model predicts the changes in invadopodia dynamics upon inhibition of myosin, adhesions, and the Rho-Rho-associated kinase (ROCK) pathway. Altogether, our work highlights the role of matrix plasticity in invadopodia dynamics and can help design dissipative biomaterials to modulate cancer cell motility.
Powder X-ray diffraction of 1-(4-Nitrophenyl)-2-piperidinone, C11H12N2O3

X-ray powder diffraction data for 1-(4-Nitrophenyl)-2-piperidinone, C11H12N2O3, are reported [a = 9.514(3) Å, b = 12.308(6) Å, c = 9.175(1) Å, α = 90°, β = 91.811(2)°, γ = 90°, V = 1073.94 Å3, Z = 4, ρ cal = 1.362 g cm−3 and space group P21/n]. All measured lines were indexed and are consistent with the P21/n space group. No detectable impurities were observed.
Inhibition of MK2 kinase as a potential therapeutic target to control neuroinflammation in Alzheimer’s disease

Alzheimer’s disease (AD) is one of the most prevalent neurodegenerative diseases [1,2]. The disease affects a global population of over 40 million, along with a burden of 7.7 million new cases every year. The two core pathophysiological hallmarks of AD include the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles (NFT) in the brain. The currently approved treatments provide only a symptomatic relief, but fail to modify the pathological features of AD [3,4]. Moreover, a single class of therapy may not be suitable due to the multifactorial nature of AD. Thus, there is an essential need to examine the option of effective combination therapies to be administered prior to cognitive loss. Several models of AD display sustained and chronic inflammatory biomarkers in brain tissues along with the pathological hallmarks. These biomarkers modulate the pathological hallmarks of AD leading to progressive neurodegeneration and are linked directly to memory impairment, cognitive deficits, and dementia. Thus, chronic neuroinflammation has emerged as a third important core pathological feature of AD. The microglia, astrocytes, and other neuronal cells are normally involved in neuroprotective functions by generating anti-Aβ antibodies and promoting CNS to peripheral clearance of excess Aβ in the brain [5,6]. However, during the excess cerebral load of Aβ deposits and soluble Aβ42 in the brain, the toll-like receptors (TLR4) present on the surface of these neuronal cells get activated. This further modulates p38MAPK (p38 mitogen-activated protein kinase) pathway resulting into a downstream activation of MK2 (MAPKAPK2, MAPKactivated protein kinase 2) in CNS. The activation of MK2 primarily regulates the transcriptional activation of proinflammatory cytokines, chemokines, and neurotoxic substances in the brain. The mediators are involved in amplifying the inflammatory responses and, in turn, lead to microglial auto-activation [7,8]. Thus, MK2 is thought to play a central role in regulation of chronic neuronal neuroinflammation in the brains and, therefore, can be a novel and attractive therapeutic target in AD. 2. MK2 biology and its relevance in Alzheimer’s disease
Evaluation of the diagnostic performance of nine commercial RT‐PCR kits for the detection of SARS‐CoV‐2 in Colombia

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic has led to the design and development of multiple reverse‐transcription polymerase chain reaction kits aimed to facilitate the rapid scale‐up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS‐CoV‐2 genetic variability and its potential impact in current molecular diagnostic assays.
Effect of focused ultrasound cavitation augmented with aerobic exercise on abdominal and intrahepatic fat in patients with non-alcoholic fatty liver disease: A randomized controlled trial

Objectives The study aimed to examine the effect of focused ultrasound cavitation augmented with aerobic exercise on localized abdominal and intrahepatic fat in fatty liver patients. Methods 34 fatty liver patients aged 30–45 with a body mass index (BMI) of 30–40 kg/m2 were randomly assigned into two equally numbered groups. Group A received focused ultrasound cavitation and moderate aerobic exercise for three months, while Group B (control group) received moderate aerobic exercise only. Abdominal subcutaneous fat volume, visceral fat volume, liver-to-spleen ratio (L/S ratio), body weight, BMI, and waist circumference were measured both before and after the study period. Results Both groups showed significant improvements in subcutaneous fat volume, visceral fat volume, body weight, BMI, and waist circumference relative to baseline where (P < 0.001), with a higher percentage in group A. L/S ratio only showed a significant improvement in group A. Between-group differences were noteworthy regarding L/S ratio and waist circumference where (P < 0.0001). Conclusion While substantial risky measures in non-alcoholic fatty liver disease have been modified by aerobic exercise, its combination with focused ultrasound cavitation causes more notable effects on the reduction of abdominal and intrahepatic fat, making it a superior option. Trial registration ClinicalTrials.gov: NCT04161703
Challenges in the diagnostic approach of suspected pulmonary embolism in COVID-19 patients

ABSTRACT Accumulating studies on COVID-19 patients report high incidences of thrombotic complications, but guidance on the best diagnostic approach for suspected pulmonary embolism (PE) in COVID-19 patients is lacking. Diagnosing PE in these patients is challenging as signs and symptoms of PE and COVID-19 show wide overlap, D-dimer levels are often elevated in the absence of thrombosis and computed tomography pulmonary angiography (CTPA) may be unfeasible in the case of severe renal impairment and/or hemodynamic instability. This narrative review discusses available literature and guidelines on current diagnostic algorithms for suspected PE in special patient populations, in particular COVID-19. A special focus is on reviewing the literature aimed at identifying symptoms with a high suspicion for PE and on the diagnostic performance of diagnostic algorithms for suspected PE in the setting of COVID-19. Based on available literature, the index of suspicion for PE should be high in the case of unexplained abrupt worsening of respiratory status, typical symptoms of deep-vein thrombosis and/or acute unexplained right ventricular dysfunction. Despite the lack of prospective diagnostic management studies, we propose to adhere to current diagnostic algorithms applying assessment of pretest probability and D-dimer testing as available evidence suggests that these might be considered safe. Preferably, algorithms using adjusted D-dimer thresholds are recommended as it likely improves the yield of the clinical decision rule/D-dimer combination.
Which Aspects of the CanMEDS Competencies are Most Valued in Radiologists? Perspectives of Trainees From Other Specialties

Purpose: Radiologists work primarily in collaboration with other healthcare professionals. As such, these stakeholder perspectives are of value to the development and assessment of educational outcomes during the transition to competency-based medical education. Our aim in this study was to determine which aspects of the Royal College CanMEDS competencies for diagnostic radiology are considered most important by future referring physicians. Methods: Institutional ethics approval was obtained. After pilot testing, an anonymous online survey was sent to all residents and clinical fellows at our university. Open-ended questions asked respondents to describe the aspects of radiologist service they felt were most important. Thematic analysis of the free-text responses was performed using a grounded theory approach. The resulting themes were mapped to the 2015 CanMEDS Key Competencies. Results: 115 completed surveys were received from residents and fellows from essentially all specialties and years of training (out of 928 invited). Major themes were 1) timeliness and accessibility of service, 2) quality of reporting, and 3) acting as a valued team member. The competencies identified as important by resident physicians were largely consistent with the CanMEDS framework, although not all key competencies were covered in the responses. Conclusions: This study illustrates how CanMEDS roles and competencies may be exemplified in a concrete and specialty-specific manner from the perspective of key stakeholders. Our survey results provide further insight into specific objectives for teaching and assessing these competencies in radiology residency training, with the ultimate goal of improving patient care through strengthened communication and working relationships.
Using a Web-Based Quiz Game as a Tool to Summarize Essential Content in Medical School Classes: Retrospective Comparative Study

Background Kahoot! is a web-based technology quiz game in which teachers can design their own quizzes via provided game templates. The advantages of these games are their attractive interfaces, which contain stimulating music, moving pictures, and colorful, animated shapes to maintain students’ attentiveness while they perform the quizzes. Objective The aim of this study was to evaluate the use of Kahoot! compared with a traditional teaching approach as a tool to summarize the essential content of a medical school class in the aspects of final examination scores and the perception of students regarding aspects of their learning environment and of process management. Methods This study used an interrupted time series design, and retrospective data were collected from 85 medical students. Of these 85 students, 43 completed a Kahoot! quiz, while 42 students completed a paper quiz. All students attended a lecture on the topic of bone and joint infection and participated in a short case discussion. Students from both groups received the same content and study material, with the exception that at the end of the lesson, students in the Kahoot! group completed a quiz summarizing the essential content from the lecture, whereas the other group received a paper quiz with the same questions and the teacher provided an explanation after the students had finished. The students’ satisfaction was evaluated after the class, and their final examination was held 2 weeks after the class. Results The mean final examination score in the Kahoot! group was 62.84 (SD 8.79), compared to 60.81 (SD 9.25) in the control group (P=.30). The students’ satisfaction with the class environment, learning process management, and teacher were not significantly different between the 2 groups (all P>.05). Conclusions In this study, it was found that using Kahoot! as a tool to summarize the essential content in medical school classes involving a lecture and case discussion did not affect the students’ final examination scores or their satisfaction with the class environment, learning process management, or teacher.
Ethics education in clinical pastoral education: prevalence and types

Abstract Chaplains frequently serve on ethics committees, as ethics consultants, and as Institutional Review Board (IRB) members in hospitals. However, little is known about how Clinical Pastoral Education (CPE) residents are trained in ethics and whether this training is appropriate or adequate for chaplains' subsequent work in health care settings. We created a survey to canvas 222 CPE residency programs in the United States accredited by the ACPE: The Standard for Spiritual Care (ACPE) to inquire about the prevalence of ethics curricula within residency programs, the educational structure of ethics curricula, and challenges associated with teaching ethics within CPE. We received a total of 84 responses for a 38% response rate. Of these, three-quarters of the programs had a required ethics curriculum, another 10% were in the process of developing one, and 18% had none. There was a great deal of variability in the ethics curricula among the different programs. Developing guidelines for a standardized ethics curriculum could help healthcare chaplains provide more effective service on ethics committees, as ethics consultants, and as IRB members.
Calciomurmanite, (Na,□)2Ca(Ti,Mg,Nb)4[Si2O7]2O2(OH,O)2(H2O)4, a new mineral from the Lovozero and Khibiny alkaline complexes, Kola Peninsula, Russia

The new mineral calciomurmanite, (Na,□)2Ca(Ti,Mg,Nb)4[Si2O7]2O2(OH,O)2(H2O)4, a Na-Ca ordered analogue of murmanite, was found in three localities at Kola Peninsula, Russia: at Mt. Flora in the Lovozero alkaline complex (the holotype) and at Mts. Eveslogchorr (the cotype) and Koashva, both in the Khibiny alkaline complex. Calciomurmanite is a hydrothermal mineral formed as a result of late-stage, low-temperature alteration (hydration combined with natural cation exchange) of a high-temperature, anhydrous phosphate-bearing titanosilicate, most likely lomonosovite and/or betalomonosovite, in the peralkaline (hyperagpaitic) rocks. The holotype sample is associated with microcline, aegirine, lorenzenite and fluorapatite, whereas the cotype sample occurs with microcline, aegirine, lamprophyllite, tsepinite-Ca and tsepinite-K. The mineral occurs as lamellae up to 0.1 × 0.4 × 0.6 cm, sometimes combined in fan-shaped aggregates up to 3.5 cm. Calciomurmanite is pale brownish or purple; the streak is white. The lustre is nacreous on cleavage surface and greasy on broken surface across cleavage. The (001) cleavage is perfect, mica-like; the fracture is stepped. D meas = 2.70(3), D calc = 2.85 g cm−3. The mineral is optically biaxial (–), α = 1.680(4), β = 1.728(4), γ = 1.743(4), 2 V meas = 58(5)°. The IR spectrum is reported. The chemical composition (wt%, electron-microprobe data, H2O by the Alimarin method) is: Na2O 5.39, K2O 0.30, CaO 7.61, MgO 2.54, MnO 2.65, FeO 1.93, Al2O3 0.85, SiO2 30.27, TiO2 29.69, Nb2O5 6.14, P2O5 0.27, H2O 11.59, total 99.23. The empirical formula of the holotype sample, calculated on the basis of Si + Al = 4 apfu , is: Na1.34Ca1. 04K0.05Mg0.49Mn0.29Fe0. 21Nb0.36Ti2.85(Si3.87Al0.13)Σ4O16. 40(OH)1.60(PO4)0.03(H2O)4.94. Calciomurmanite is triclinic, P -1, a = 5.3470(6), b = 7.0774(7), c = 12.146(1) A, α = 91.827(4), β = 107.527(4), γ = 90.155(4)°, V = 438.03(8) A3 and Z = 1. The strongest reflections of the X-ray powder pattern [ d ,A( I )( hkl )] are: 11.69(100)(001), 5.87(68)(011, 002), 4.25(89) (-1-11, -111), 3.825(44)(-1-12, 003, -112), 2.940(100)(-1-21, -121), 2.900(79)(004, 120). The crystal structure was solved by direct methods from single-crystal low-temperature (200 K) X-ray diffraction data and refined to R = 0.0656 for the holotype and 0.0663 for the cotype. The structure is based on a three-sheet HOH block: an octahedral ( O ) sheet containing alternating chains of NaO6 and TiO6 octahedra and two heteropolyhedral ( H ) sheets consisting of Si2O7 groups, TiO6 octahedra and CaO8 polyhedra. H2O molecules occupy two sites in the interlayer space.
Leader-member exchange fosters nurses’ job and life satisfaction: The mediating effect of job crafting

This study aims to evaluate the effects of leader-member exchange (LMX) on job and life satisfaction among nurses in China and to examine the mediating effect of individual and collaborative job crafting between LMX and job and life satisfaction. The study recruited 263 nurses who worked in hospitals in Zhejiang province, China. A set of self-administered questionnaires were used to measure the variables of LMX, job crafting, job and life satisfaction. The data was analyzed using the partial least square structural equation modelling (PLS-SEM). The results reveal that LMX has a significant positive influence on job crafting and job satisfaction. Collaborative job crafting has a significant positive influence on the job satisfaction of nurses, whereas individual job crafting does not. Moreover, LMX will affect job satisfaction and life satisfaction through a partial mediating effect of both individual and collaborative job crafting. Finally, the article discusses the academically and practical implications, and also provide some suggestions and directions for the future research.
Vanadium oxides modify the expression levels of the p21, p53, and Cdc25C proteins in human lymphocytes treated in vitro

In vitro assays have demonstrated that vanadium compounds interact with biological molecules similar to protein kinases and phosphatases and have also shown that vanadium oxides decrease the proliferation of cells, including human lymphocytes; however, the mechanism, the phase in which the cell cycle is delayed and the proteins involved in this process are unknown. Therefore, we evaluated the effects of vanadium oxides (V2O3, V2O4 and V2O5) in human lymphocyte cultures (concentrations of 2, 4, 8, or 16 μg/ml) on cellular proliferation and the levels of the p53, p21 and Cdc25C proteins. After 24 h of treatment with the different concentrations of vanadium oxides, the cell cycle phases were determined by evaluating the DNA content using flow cytometry, and the levels of the p21, p53 and Cdc25C proteins were assessed by Western blot analysis. The results revealed that the DNA content remained unchanged in every phase of the cell cycle; however, only at high concentrations did protein levels increase. Although, according to previous reports, vanadium oxides induce a delay in proliferation, DNA analysis did not show this occurring in a specific cell cycle phase. Nevertheless, the increases in p53 protein levels may cause this delay.
Validation of CP‐GEP (Merlin Assay) for predicting sentinel lymph node metastasis in primary cutaneous melanoma patients: A U.S. cohort study

Approximately 85% of melanoma patients who undergo a sentinel lymph node biopsy (SLNB) are node‐negative. Melanoma incidence is highest in patients ≥65 years, but their SLNB positivity rate is lower than in younger patients. CP‐GEP, a model combining clinicopathologic and gene expression variables, identifies primary cutaneous melanoma (CM) patients who may safely forgo SLNB due to their low risk for nodal metastasis. Here, we validate CP‐GEP in a U.S. melanoma patient cohort.
Ptpn2 and KLRG1 regulate the generation and function of tissue-resident memory CD8+ T cells in skin

Hochheiser et al. identify Ptpn2 and KLRG1 as new regulators of CD103+ CD8+ TRM cells in mouse skin. While KLRG1 inhibits TRM formation, genetic Ptpn2 deficiency increases the protective function of TRM cells in infection but aggravates TRM-dependent autoimmunity.
Multiple carbon incorporation strategies support microbial survival in cold subseafloor crustal fluids

Quantitative assessments of microbial activity in crustal fluids yield potential strategies to supplement recalcitrant DOC. Biogeochemical processes occurring in fluids that permeate oceanic crust make measurable contributions to the marine carbon cycle, but quantitative assessments of microbial impacts on this vast, subsurface carbon pool are lacking. We provide bulk and single-cell estimates of microbial biomass production from carbon and nitrogen substrates in cool, oxic basement fluids from the western flank of the Mid-Atlantic Ridge. The wide range in carbon and nitrogen incorporation rates indicates a microbial community well poised for dynamic conditions, potentially anabolizing carbon and nitrogen at rates ranging from those observed in subsurface sediments to those found in on-axis hydrothermal vent environments. Bicarbonate incorporation rates were highest where fluids are most isolated from recharging bottom seawater, suggesting that anabolism of inorganic carbon may be a potential strategy for supplementing the ancient and recalcitrant dissolved organic carbon that is prevalent in the globally distributed subseafloor crustal environment.
Religious Involvement, Depressive Symptoms, and Burden in Caregivers of Terminally Ill Patients

Caring for terminally ill patients can be emotionally burdensome. Previous research has demonstrated that caregiving is associated with anxiety and depression. Research on caregivers of terminally ill patients is limited by retrospective studies with small samples. This study aimed to (1) describe religiosity, religious coping, and depressive symptoms in caregivers of persons with amyotrophic lateral sclerosis or advanced cancer; (2) examine the relationship between religiosity and depressive symptoms in caregivers of persons with amyotrophic lateral sclerosis or advanced cancer; (3) examine the relationship between religious coping and depressive symptoms in caregivers of persons with amyotrophic lateral sclerosis or advanced cancer. A descriptive exploratory design was used to analyze data from a larger 5-year National Institutes of Health–funded multisite randomized controlled trial (the TAILORED study). Nearly half of the caregivers screened positive for depressive symptoms, and negative religious coping was associated with higher depressive symptoms (P < .001). Spouse caregivers reported higher depressive symptoms than nonspouse caregivers. Many caregivers experienced depression, which was mitigated by positive religious coping.
AXIN1 knockout does not alter AMPK/mTORC1 regulation and glucose metabolism in mouse skeletal muscle

Tamoxifen‐inducible skeletal muscle‐specific AXIN1 knockout (AXIN1 imKO) in mouse does not affect whole‐body energy substrate metabolism. AXIN1 imKO does not affect AICAR or insulin‐stimulated glucose uptake in adult skeletal muscle. AXIN1 imKO does not affect adult skeletal muscle AMPK or mTORC1 signalling during AICAR/insulin/amino acid incubation, contraction and exercise. During exercise, α2/β2/γ3AMPK and AMP/ATP ratio show greater increases in AXIN1 imKO than wild‐type in gastrocnemius muscle.