Miracle-dz/UniFAR-FTUs huggingface dataset:
Phenotypic characterization of the wild boar populations from the Sovata forest district, based on age and sex.

In order to compile a phenotypic characterization of the wild boar, the number of wild game was evaluated during the period between February 14 and March 29 of the years 2009 and 2010 within two hunting funds belonging to the Sovata Forest District, Niraj 42 and Sebeş 43 hunting funds, allowing the elaboration of a technical data sheet used in the experiment. Measurements of the individuals were conducted during 2008, 2009 and 2010 and several traits were noted: body length, withers height, croup height, thorax perimeter, body weight, skull length, forehead width between the ears. The age of the boars was estimated based on dentition. They were then classified in three categories: youth (7-12 months), animals between 1 and 2.5 years and animals over 3 years of age. The results show that the body length of the animals establishes a strong difference between males and females, favoring the males after the age of 1.5 2 years. Measurements of the withers height showed modifications according to age until the reaching of full body maturity (3 years). Results on the croup height underline a difference given by age and sex reaching from a mean value of 58 cm in males and 56 cm in females in the first year of age, to 86 cm in males and 81 cm in females over 3 years of age. The highest thorax perimeter values were observed in the males over 3 years of age, with a mean value of 137 cm, the females exhibiting a value of only 121 cm. Because the body weight has a complex genetic determinism, being highly influenced by the soil bonitation, the existence of complementary nourishment and the mating season the trait did not condition in any way the value of the trophy at the sampling time.
Human Leukocyte Antigen: Class I Allele Frequencies and Haplotype Distributionin a Tertiary Care Hospital in Bangladesh

Human leukocyte antigen (HLA) are cell surface glycoproteins encoded by Major Histocompatibility Complex (MHC) geneof human genome. HLA antigen frequency and haplotype distribution are useful for determining disease associations, origin, migration and genetic relationships between populations and predicting the outcome of transplantation. Thus, the present study was carried outto identify HLA class I (HLA-A and HLA-B) antigen and haplotype distribution among a selected Bangladeshi population. This retrospective study was conducted among 1070 individuals who were referred by cliniciansfor HLA typing at the Tissue Typing Laboratory of the Department of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU) during the period 2009 to 2011. For HLA typing, Blood was collected in heparin containing tube and the laboratory tests were performed by the microlymphocytotoxicity technique according to manufacturer’s instructions.Out of 19 HLA-A and 37HLA-B antigens tested, a total of 19/19 and 36/37 antigens were detectedrespectively in this study. The most frequent antigens of HLA-A and HLA-B detected were A11 (25.4%), A24 (16.6%), B75 (18.1%) and B35 (11.3%). The least antigen frequency detected for HLA-A locus were A69 (0.09%), A26 (0.28%), A34 (0.28%), while for HLA-B locus were B81 (0.09%) and B56 (0.09%). Among the HLA-A and HLA-B antigens, some alleles were found to be homozygote such as A11 (4.0%), A2 (2.7%), A24 (2.1%) andB75 (2.4%), B35 (1.8%), and B44 (1.4%) respectively. The most frequent haplotype in the study populationwereA11: B75 (4.9%). The most frequent antigens of HLA-A and HLA-B detected were A11 (25.4%), B75 (18.1%) respectively. The distribution of HLA haplotypes among the study population indicates that it has the influence of Oriental and Asian populations. Thus, this study will be helpful to provide valuable information for population genetics and HLA disease association analysis.Bangladesh Med Res Counc Bull 2018; 44(1):01-08
Phenotypic and molecular identification of carbapenemase-producing Enterobacteriaceae - challenges in diagnosis and treatment

Abstract Introduction: Infections due to carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CPCRE) are an emerging global public health threat. The purpose of this study was to investigate phenotypic and genotypic features of CP-CRE strains isolated from hospitalized patients. Material and methods: Between 1st of January - 1st of July 2017, in the Department of Microbiology, “Dr. Constantin Opriş” County Emergency Hospital Baia Mare, Romania, 1110 strains of Enterobacteriaceae were isolated from bronchial secretions, urine, wounds and blood cultures. Bacterial identification and antimicrobial susceptibility tests were performed by conventional methods, Vitek 2 Compact and M.I. C.E. strips. We analysed all Enterobacteriaceae strains non-susceptible to carbapenems according to CLSI 2017 criteria. The modified Hodge test (MHT), the modified carbapenem inactivation method (mCIM) and the combination disks test (KPC, MBL, OXA-48 Confirm kit, Rosco Diagnostica) were used for phenotypic confirmation, whereas a multiplex PCR assay for genes blaKPC, blaNDM and blaOXA-48 was used for genetic confirmation. Results: 19 non-duplicate strains isolated from 16 patients were phenotypically identified as CP-CRE: Klebsiella pneumoniae (n=14), Escherichia coli (n=2), Providencia stuartii (n=2) and Serratia marcescens (n=1). Most strains were isolated from bronchial secretions (n=9). The carbapenem-hydrolizing enzymes were identified by the combination disks test as: KPC (n=9), OXA-48-like (n=5) and MBL (n=5). Molecular confirmation was performed in 18 phenotypically positive isolates with 100% concordant results with mCIM and combination disks test. Discrepant results were noticed with the MHT in case of 4 NDM-producers confirmed by PCR. All CP-CRE strains were resistant to all tested cephems. Three out of 9 K. pneumoniae strains tested against colistin were found resistant. Conclusions: The most common carbapenemase detected was KPC. Therapeutic options were limited in all positive cases. Rapid and reliable detection of CP-CRE is critical for preventing the spread of these pathogens
The Prognostic Impact of Trastuzumab Resistance and Body Composition : Parameters in Metastatic Breast Cancer

textabstractThe aim of this thesis was to evaluate the impact of several patient- and treatment related factors on the outcome of patients with metastatic breast cancer. The efficacy of anti-Her2 agents in the treatment of Her2 positive MBC is well established across clinical trials. However, the field of Her2-targeted therapy in Her2 positive MBC is rapidly evolving and sometimes previous cohorts of patients in clinical trials are therefore not representative anymore for the current daily clinical practice. In general, most patients in clinical trials were trastuzumab-naive before enrollment in the study according to current guidelines. As a result, the efficacy of retreatment with anti-Her2 agents after progressive disease on previous Her2- targeted therapy with the same agents remains unclear.
Seasonal variations of the yield and proximate composition of freshwater crayfish, Astacus leptodactylus (Eschscholtz, 1823).

In this study, proximate composition and meat yield of freshwater crayfish (Astacus leptodactylus) caught from various lakes in Tekirdag were investigated regarding seasonal variations. It was determined that, meat yield of the samples was highest in summer (19.55%) and lowest in spring (14.84%), and this difference was statistically important (p 0.05) between the protein, moisture and ash contents of the samples regarding the seasons, and their average amounts were 13.96%, 81.76% and 1.72%, respectively. The maximum and minimum lipid content was estimated to be 1.31% in spring and 0.53% in winter, respectively (p< 0.05). The average amount of carbohydrates was 1.79%, and energy value of the freshwater crayfish was 94.51 Kcal/100g. The lowest levels of carbohydrates and energy were estimated in spring and winter respectively (p<0.05).
A new species of Tityus C. L. Koch, 1836 (Scorpiones: Buthidae) from Dominican Amber

Tityus azari sp. n. , a new species of fossil scorpion belonging to the genus Tityus C. L. Koch, 1836 is described based on a specimen in amber from the Dominican Republic. Although the new species can be associated with the extant fauna of the Neotropical region, it presents some particular morphological features such as the presence of sharp denticles on the edge of pedipalp fingers. Due to the incompleteness of the specimen it cannot be assigned to any precise extant subgenera. Once again, this discovery attests to a considerable degree of diversity in the Dominican amber-producing forests.
A New nutritional Approach for promoting Gut Health and Animal Performance

The antisecretory factor (AF) is a protein secreted in plasma and other tissue fluids in mammalians which was shown to be a potent inhibitor of intestinal fluid secretion and inflammation. After the AGP ban, several nutritional approaches aimed to the optimization of the weaning transition and reduction of gut diseases have been proposed, but the success rates are really low compared to antimicrobials. AF-inducing diets appear to be suitable alternatives to AGP. Indeed AF content in sows’ colostrum and milk appears to be a protection factor against diarrhoea in suckling piglets. Increased AF level in plasma by dietary means, such as feeding hydro-thermally processed cereals (HPC), has been demonstrated in human and animals. We tested the effect of two different HPC level of inclusion in a wheat-barley based diet on weaned piglets growth performance, reared in an experimental farm. The results confirmed the efficacy of HPC as growth promoters in piglet nutrition: HPC supplementation improved ADG and FCR. In vitro test were made to study the antisecretory and anti-inflammatory properties of the AF protein and its mechanism of action by using AF-16, the active region of the protein. The Ussing chamber experiments performed on polarized IPEC-J2 cells confirmed the neuronal involvement in the antisecretory activity of AF. In fact, AF-16 did not inhibit CT-induced Isc. A slower rate of Isc increase was observed during the simultaneous administration with AF-16 and CT. High dosages of AF-16 were found to reduce the LPS-stimulated NO production in RAW264.7 cell, thus supporting the hypothesis of an anti-inflammatory action. On the contrary, no significant results were obtained on PMA-stimulated ROS generation in pig alveolar macrophages.
Crosstalk of cells in bone metastasis: Molecular and cellular analysis of the mutual effects of prostate cancer cells and osteoblasts

Prostate cancer preferentially metastasizes to the skeleton and abundant evidence exists that osteoblasts specifically support the metastatic process, including cancer stem cell niche formation. At early stages of bone metastasis, crosstalk of prostate cancer cells and osteoblasts through soluble molecules results in a decrease of cancer cell proliferation, accompanied by altered adhesive properties and increased expression of bone-specific genes, or osteomimicry. Osteoblasts synthesize a plethora of biologically active factors, which comprise the unique bone microenvironment. By means of quantitative real-time RT-PCR it was determined that exposure to the osteoblast secretome induced gene expression changes in prostate cancer cells, including the upregulation of osteomimetic genes such as BMP2, AP, COL1A1, OPG and RANKL. IL6 and TGFbeta1 signaling pathway components also became upregulated at early time points. Moreover, osteoblast-released IL6 and TGFbeta1 contributed to the upregulation of OPG mRNA in LNCaP. Thus, the earliest response of prostate cancer cells to osteoblast-released factors, which ultimately cause metastatic cells to assume an osteomimetic phenotype, involved activation of paracrine and autocrine IL6 and TGFbeta signaling. On the other hand, a microarray analysis showed that osteoblasts exposed to the secretome of prostate cancer cells exhibited gene expression alterations suggestive of repressed proliferation, decreased matrix synthesis and inhibited immune response, which together indicate enhanced preosteocytic differentiation. TGFbeta signaling, known to inhibit osteoblast maturation, was strongly suppressed, as shown by elevated expression of negative regulators, downregulation of pathway components and of numerous target genes. Transcriptional downregulation of osteoblast inhibitory molecules such as DKK1 and FST also occurred, with concomitant upregulation of the osteoinductive molecules ADM, STC1 and BMP2, and of the transcription factors CBFA1 and HES1, which promote osteoblast differentiation. Finally, the mRNA encoding NPPB, the precursor of a molecule implicated in the inhibition of TGFbetaeffects, in bone formation and in stem cell maintenance, became upregulated after coculture both in osteoblasts and in prostate cancer cells. These results provide an insight into potential mechanisms of dysregulated bone formation in metastatic prostate cancer, as well as mechanisms by which osteoblasts might enhance the invasive, osteomimetic and stem cell-like properties of the tumor cells. In particular, the differential modulation of TGFbetasignaling in prostate cancer cells and osteoblasts appears to merit further research.
Complications of Transcatheter Closure in Patent Ductus Arteriosus Patients

Background: Published studies have shown excellent success rates and also low rates of life-threatening complications with trans-catheter closure of patent ductus arteriosus (PDA). However, most studies to date have been conducted in developed nations and reports from developing countries such as Iran are lacking. Objectives: To report our experience with trans-catheter closure of patent ductus arteriosus (PDA) and compare two devices and complications. Patients and Methods: From 2007 to 2011, 72 consecutive PDA cases were prospectively enrolled. For PDAs ≤ 2 mm, the coiling method was employed whereas patients with PDA > 2 mm received Amplatzer duct occlusion (ADO). Success was defined as trivial residual shunt (< 1 mm) in the echocardiography performed 30 minutes after the procedure. After the procedure, the patients were admitted to the pediatric cardiology ward and observed for 72 hours during which the occurrence of major and minor complications was monitored. Major complications included cardiac arrest, displacement and/or embolization of the device and patient needing blood transfusion due to severe hemorrhage. Sampling was based on convenience method and the patients who had significant pulmonary artery hypertension were excluded from our study. Statistical analyses were done using SPSS software version 23 (IBM corp., New York, United States). Continuous variables are presented as mean ± standard deviation. The Mann-Whitney U test was used to compare the differences between two independent groups. Categorical variables were depicted as proportions and compared across the intervention groups, using Chi-square with Fisher’s exact text where appropriate. In all tests, a P value < 0.05 was deemed necessary to reject the null hypothesis. Results: With the coiling (n = 39) and Amplatzer methods (n = 33), successful closure was observed totally and recovered with no major complications. Frequent complications were benign arrhythmia (44.4%), and hemorrhage in 1 (1.4%) of the patients. Complication rate did not differ between the two techniques (P > 0.05). Conclusions: Excellent and comparable success rates for both closure techniques were observed and no major complications were observed.
Is Laparoscopic Appendectomy Appropriate TreatmentFor Perforated Acute Appendicitis With Peritonitis

Acute appendicitis is a common cause of acute abdominal pain and is one of the most common abdominal surgical emergencies. In the past, the treatment of acute appendicitis consisted of open appendectomy through a small skin incision in the right lower abdominal quadrant. In some cases of perforated appendicitis with diffuse peritonitis, median laparotomy was performed with appendectomy, irrigation and drainage of the abdominal cavity. Nowadays, surgical treatment of acute appendicits has shifted from open appendectomy to laparoscopic appendectomy in adults and in pediatric patients [1].
VALIDATION OF VAGINAL SELF-SAMPLING AS AN ALTERNATIVE OPTION IN PCR BASED DETECTION OF HPV IN CERVICAL CANCER SCREENING IN BOSNIA AND HERZEGOVINA

Cervical cancer represents a serious health problem affecting women worldwide especially in developing countries due to low socioeconomic status, inadequate health-care infrastructure, weaknesses in education on this particular issue and lack of effective screening programmes. The primary aim of this study was to assess alternative screening method for the improvement of cervical cancer prevention in conditions of Bosnia and Herzegovina (B&H), which could be applicable in other developing countries as well. The study was conducted on 101 subjects who provided their self-sampled vaginal swabs and/or cervical specimens collected by their gynecologists. Universal Human Papilloma Virus (HPV) primer set optimized to detect a wide range of HPV types was used for HPV genotyping from obtained swab samples in multiplex PCR. Amplicons were analyzed in agarose gel and Agilent 2100 bioanalyzer – a platform based on microfluid technology. Inter-rater agreement kappa (MedCalc2) was used to assess concordance between results of cervical and vaginal sample analysis. Out of 39 subjects who provided their vaginal and cervical samples, results of HPV detection mismatched in 10% of the cases. Inter-rater agreement showed good strength of coincidence between the results of cervical and vaginal sample analysis (kappa=0,748, CI=95 %). We presented an alternative PCR method for the detection of HPV based on vaginal self sampling which is affordable, informative, simple and applicable with high coverage level of defined targeted population and potentially significant in the given cultural and socioeconomic context.
A version of the Hörmander–Malliavin theorem in 2-smooth Banach spaces

We consider a stochastic evolution equation in a 2-smooth Banach space with a densely and continuously embedded Hilbert subspace. We prove that under Hormander's bracket condition, the image measure of the solution law under any finite-rank bounded linear operator is absolutely continuous with respect to the Lebesgue measure. To obtain this result, we apply methods of the Malliavin calculus.
Stability and Accuracy Analysis of Coarse-Grain Viscoelastic Simulations

We analyze the stability and accuracy of the coarse-grain memory variable technique used for viscoelastic wave-field simulations. Our analysis shows that the general behavior of the coarse-grain system is well described by effective parameters ( M E and Q E ) that are derived from the harmonic average of the moduli over the volume of the coarse-grain cell. The use of these effective parameters proves essential for analyzing the performance and accuracy of the coarse-grain system for Q values less than about 20. A necessary stability condition for the coarse-grain system requires that the weighting coefficients be bounded between 0 and 1. Specifying the weights using the approach of Day and Bradley (2001) satisfies this condition for Q values of about 3 and larger; however, using unconstrained optimization techniques will often produce weights that violate this condition at much higher Q values. We also derive a variation of the original coarse-grain methodology called the element-specific modulus (ESM) formulation in which each element of the coarse-grain cell uses a different unrelaxed modulus. We demonstrate that the accuracy of the coarse-grain system for Q values lower than about 20 is significantly improved with the ESM formulation without increasing the computational cost. Finally, we present a technique for optimizing the accuracy of the coarse-grain system for very low Q based on the use of the effective quality factor ( Q E ). We demonstrate that using conventional optimization techniques that do not employ the effective parameter Q E will actually degrade the accuracy of the coarse-grain system. Manuscript received 21 March 2002.
Pharmacodynamic and Pharmacokinetic Studies on Tetracycline Hydrochloride in Rabbits

Tetracycline is one of the most important groups of antibiotics that have harmful effects on the consumers, therefore the public health safety against its residues represents a significant issue. This study aimed to estimate the effect of tetracycline hydrochloride on some hematological parameters, kidneys function tests as well as liver and breast muscle enzymes with special reference to the supposed withdrawal time of this drug in different rabbits’ tissues (kidney, liver and muscles), following oral dose of tetracycline using High Performance Liquid Chromatography. Tetracycline was administrated to eighteen rabbits directly into the stomach at a dose of 35 mg/kg BW once daily for five successive days. Samples were collected on the 1 st , 3 rd , 7 th , 14 th , 21 st and 28 th days after the last oral dose. The results revealed that, tetracycline caused a significant increase in the uric acid, urea, creatinine, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) activities with no significant changes in the hematological parameters when compared with the control group. The residues remained in the liver and kidney for 7 days, while in muscles for 3 days only after the last oral dose of the drug. In conclusion, the disturbances in the biological parameters occurred by tetracycline administration in rabbits was transient and returned to normal after 7 days of last treatment. The withdrawal time of tetracycline was 14 days from the rabbit's tissues.
Knowledge and Socioeconomic Status as The Factors of Pre-hospital Delay in Patients with Acute Coronary Syndrome

Acute Coronary Syndrome (ACS) is a cardiac emergency condition that requires fast and precise action while a pre-hospital delay in a patient to come to the ED is still common. This pre-hospital delay is related to the socioeconomic and knowledge factor. The purpose of this study is to determine the relationship of socioeconomic and knowledge with the pre-hospital delay of ACS patients to come to Integrated Heart Service in Emergency Department of Sanglah General Hospital, Denpasar. This research is a quantitative research with cross-sectional design and purposive sampling as data collection technique with 292 patients. The results showed that 55.8% of patients have low socioeconomic status and there are 71.6% of patients who do not know ACS. The pre-hospital delay, in this case, is associated with low socioeconomic status and knowledge of ACS so that health workers need to provide counseling to patients and families about ACS signs and symptoms, healthy lifestyles, and the importance of coming to theemergency department when signs of ACS are felt.
Parametric decay of magneto-acoustic oscillations in a cylindrical plasma

Parametric decay of magneto-acoustic oscillations in a cylindrical plasma column is considered, including the Hall effect and the effects of finite resistivity. Growth rates for axisymmetric modes in decay instabilities are derived and applied to the problems of parametric excitation of Alfvén waves and supplementary heating of plasmas. It is shown that both the slow (ion cyclotron) and fast (compressional) modes may be parametrically excited. The threshold &litudes for nonlinear dissipation of pump field energy are calculated and briefly discussed.