Miracle-dz/UniFAR-FTUs huggingface dataset:
Are H1 and H3 haplotypes of endothelial protein C receptor (PROCR) an important factor in contracting COVID‐19?

The development of cardiovascular disease shows increase after contracting coronavirus 2019 (COVID‐19) disease and myocardial damage is observed in patients who have had the disease severely. The relationship between genetic cardiovascular risk factors with COVID‐19 infection was investigated in our study. One hundred thirty‐five patients, 27 of whom were COVID‐19 (−) and 108 were COVID‐19 (+) patients, were included in the study. Patients were divided into three groups ([COVID‐19 [−], COVID‐19 [+] asymptomatic, and COVID‐19 [+] symptomatic + patients with pulmonary involvement]). Genetic cardiovascular risk factors were examined in blood samples taken from the patients with new generation sequencing analysis. In the clinical classification, there were no significant differences between the three groups in fibrinogen beta chain—455G>A, human platelet antigen 1 (HPA1b)/platelet receptor GPIIIa/(ITGB3) (HPA1a/b; GpIIIa; integrin beta 3 L33P), ACE I/D, AGT (M268T), AGTR1 (1166A>C), Apo E (E2/E3/E4) (rs7412, rs429358), eNOS (786T>C), eNOS (894G>T) genes (p > 0.05). However, significant differences were observed in PROCR H3 haplotype/G (endothelial protein C receptor gene [EPCR] 4600A>G [A3 haplotype]), PROCR H1 haplotype/C (EPCR 4678G>C [A1 haplotype]) genes (p < 0.05). When COVID‐19 (+) and COVID‐19 (−) groups were compared, it was observed that the infection was more common in people with PROCR H1 haplotype/C and PROCR H3 haplotype/G genotypes (p < 0.05). PROCR H1 and PROCR H3 haplotypes may be an important factor in contracting COVID‐19 disease. In people with COVID‐19 disease, revealing PROCR genetic differences and measuring sEPCR levels will be beneficial in the follow‐up of the disease.
Recoding amino acids to a reduced alphabet may increase or decrease phylogenetic accuracy.

Common molecular phylogenetic characteristics such as long branches and compositional heterogeneity can be problematic for phylogenetic reconstruction when using amino acid data. Recoding alignments to reduced alphabets before phylogenetic analysis has often been used both to explore and potentially decrease the effect of such problems. We tested the effectiveness of this strategy on topological accuracy using simulated data on four-taxon trees. We simulated alignments in phylogenetically challenging ways to test the phylogenetic accuracy of analyses using various recoding strategies together with commonly-used homogeneous models. We tested three recoding methods based on amino acid exchangeability, and another recoding method based on lowering the compositional heterogeneity among alignment sequences as measured by the Chi-squared statistic. Our simulation results show that on trees with long branches where sequences approach saturation, accuracy was not greatly affected by exchangeability-based recodings, but Chi-squared-based recoding decreased accuracy. We then simulated sequences with different kinds of compositional heterogeneity over the tree. Recoding often increased accuracy on such alignments. Exchangeability-based recoding was rarely worse than not recoding, and often considerably better. Recoding based on lowering the Chi-squared value improved accuracy in some cases but not in others, suggesting that low compositional heterogeneity by itself is not sufficient to increase accuracy in the analysis of these alignments. We also simulated alignments using site-specific amino acid profiles, making sequences that had compositional heterogeneity over alignment sites. Exchangeability-based recoding coupled with site-homogeneous models had poor accuracy for these datasets but Chi-squared-based recoding on these alignments increased accuracy. We then simulated datasets that were compositionally both site- and tree-heterogeneous, like many real datasets. The effect on accuracy of recoding such doubly problematic datasets varied widely, depending on the type of compositional tree-heterogeneity and on the recoding scheme. Interestingly, analysis of unrecoded compositionally heterogeneous alignments with the NDCH or CAT models was generally more accurate than homogeneous analysis, whether recoded or not. Overall, our results suggest that making trees for recoded amino acid datasets can be useful, but they need to be interpreted cautiously as part of a more comprehensive analysis. The use of better fitting models like NDCH and CAT, which directly account for the patterns in the data, may offer a more promising long-term solution for analysing empirical data.
Soluble RAGE Prevents Type 1 Diabetes Expanding Functional Regulatory T Cells

Type 1 diabetes is an autoimmune disease with no cure, where clinical translation of promising therapeutics has been hampered by the reproducibility crisis. Here, short-term administration of an antagonist to the receptor for advanced glycation end products (sRAGE) protected against murine diabetes at two independent research centers. Treatment with sRAGE increased regulatory T cells (Tregs) within the islets, pancreatic lymph nodes, and spleen, increasing islet insulin expression and function. Diabetes protection was abrogated by Treg depletion and shown to be dependent on antagonizing RAGE with use of knockout mice. Human Tregs treated with a RAGE ligand downregulated genes for suppression, migration, and Treg homeostasis (FOXP3, IL7R, TIGIT, JAK1, STAT3, STAT5b, CCR4). Loss of suppressive function was reversed by sRAGE, where Tregs increased proliferation and suppressed conventional T-cell division, confirming that sRAGE expands functional human Tregs. These results highlight sRAGE as an attractive treatment to prevent diabetes, showing efficacy and reproducibility at multiple research centers and in human T cells.
Exploratory study of fathers providing Kangaroo Care in a Neonatal Intensive Care Unit.

AIM AND OBJECTIVES To explore fathers' views and experiences of providing Kangaroo Care (KC) to their baby cared for in a Neonatal Intensive Care Unit (NICU). BACKGROUND Kangaroo Care has been known to improve the health outcome for preterm, low birth weight and medically vulnerable term infants and achieve the optimal perinatal health wellbeing for parents and infants. Historically, mothers are considered as the dominant KC providers, whereas fathers are spectators and have been overlooked. Little is known about the fathers' perspectives in providing KC in NICUs. METHODS Individual semi-structured interviews were conducted with 10 fathers who delivered KC to their baby when in the NICU. Data were analysed using Braun and Clarke's six-phase thematical framework. The Consolidated Criteria for Reporting Qualitative Research (COREQ) checklist was followed to report this qualitative study. FINDINGS Fathers in this study identified they were passing a silent language of love and connecting with their baby by the act of KC in a challenging environment. Three themes emerged: 'Positive psychological connection', 'Embracing father-infant Kangaroo Care' and 'Challenges to father-infant Kangaroo Care'. CONCLUSION The findings of this study show KC enhances the bonding and attachment between fathers and infants. The conceptualisation of the paternal role in caregiving to a newborn is evolving as a contemporary practice. Further research is warranted to confirm or refute the study findings. Policies and facilities should be modified to include father-infant KC within the fields of neonatal care. RELEVANCE TO CLINICAL PRACTICE It is important for nurses and other health professionals to support and enable fathers to give KC. Father-infant KC is recommended in neonatal care settings.
Subarctic soil carbon losses after deforestation for agriculture depend on permafrost abundance

The northern circumpolar permafrost region is experiencing considerable warming due to climate change, which is allowing agricultural production to expand into regions of discontinuous and continuous permafrost. The conversion of forests to arable land might further enhance permafrost thaw and affect soil organic carbon (SOC) that had previously been protected by frozen ground. The interactive effect of permafrost abundance and deforestation on SOC stocks has hardly been studied. In this study, soils were sampled on 18 farms across the Yukon on permafrost and non‐permafrost soils to quantify the impact of land‐use change from forest to cropland and grassland on SOC stocks. Furthermore, the soils were physically and chemically fractionated to assess the impact of land‐use change on different functional pools of SOC. On average, permafrost‐affected forest soils lost 15.6 ± 21.3% of SOC when converted to cropland and 23.0 ± 13.0% when converted to grassland. No permafrost was detected in the deforested soils, indicating that land‐use change strongly enhanced warming and subsequent thawing. In contrast, the change in SOC at sites without permafrost was not significant but had a slight tendency to be positive. SOC stocks were generally lower at sites without permafrost under forest. Furthermore, land‐use change increased mineral‐associated SOC, while the fate of particulate organic matter (POM) after land‐use change depended on permafrost occurrence. Permafrost soils showed significant POM losses after land‐use change, while grassland sites without permafrost gained POM in the topsoil. The results showed that the fate of SOC after land‐use change greatly depended on the abundance of permafrost in the pristine forest, which was driven by climatic conditions more than by soil properties. It can be concluded that in regions of discontinuous permafrost in particular, initial conditions in forest soils should be considered before deforestation to minimize its climate impact.
Working memory impairment in relation to the severity of anxiety symptoms

ABSTRACT A working memory (WM) deficit is a reliable observation in people experiencing anxiety. Whether the level of anxiety is related to the severity of WM difficulties is still an open question. In the present experiment, we investigated this aspect by testing the WM performance of people with different levels of anxiety symptoms. Participants were grouped according to self-report anxiety into a control group with low anxiety scores and an experimental group with clinically relevant anxiety. The experimental group was then divided into a high anxiety group and a severe anxiety group. Participants performed a battery of WM tasks tagging different WM processes. The results showed that, compared to participants with low anxiety, participants with clinically relevant anxiety scores had reduced accuracy in all the WM tasks. Interestingly, participants with high and severe anxiety did not present any significant difference. Anxious participants showed difficulties also in cognitive domains other than WM. Hence, these results supply reliable evidence that people with clinically relevant anxiety scores present WM difficulties, irrespective of symptoms severity. The observation that anxiety compromises performance also in cognitive domains other than WM suggests that the deficit might affect fluid cognition.
Aggressive behaviour in antipsychotic‐naive first‐episode schizophrenia patients, their unaffected siblings and healthy controls

While patients with schizophrenia are more likely to be victims rather than perpetrators of aggressive behaviour, prior research has shown increased rates of aggressive behaviours in these patients that appear very early in the course of illness. We aimed to assess aggression in antipsychotic‐naive first‐episode schizophrenia patients, their healthy siblings, and controls; and to investigate correlates of aggression in the patients group.
Investigating the impacts of working at home among office workers with neck pain on health status, depression and sleep quality during the COVID-19 pandemic

Objective. This study aimed to assess the effect of workplace and ergonomic features on neck pain intensity, sleep problems, depression and health status in office workers during the COVID-19 pandemic. Methods. One-hundred and thirty-nine office workers with neck pain aged 18–55 years were enrolled in the study. Sociodemographic features including ergonomic features of the workplace were evaluated. The Beck depression inventory (BDI), Nottingham health profile (NHP), Pittsburgh sleep quality index (PSQI) and neck pain and disability scale (NPDS) were administered to all participants. Results. All participants were grouped based on workplace into Group 1 (working in office, n = 84) and Group 2 (working at home, n = 55). There was a statistically significant difference between the groups according to the rate of absence of back support and flexible back (respectively p = 0.020 and p = 0.029). The neck pain and disability index (NPDI) scores of the participants who work with a computer for >3 h without a break was higher in Group 2 than in Group 1, and the differences between groups were statistically significant (p = 0.001). Conclusions. The health-related quality of life was worse and neck pain intensity was severe in the working at home group than in the working in office group. Trial registration: ClinicalTrials.gov identifier: NCT05074407.
Enhancement of behavioral nociceptive responses but not itching responses by viewing mirror images in adult mice

Can mice recognize themselves in a mirror? The answer is unclear. Previous studies have reported that adult mice – when shown itch-like videos - demonstrated itch empathy. However, this was proven to be unreproducible in other studies. In the present study, we wanted to examine whether adult mice were able to recognize their mirror image. In our testing, we found that mice spent more time in the central area in an open field with mirrors surrounding the chamber than those in a normal open field. In a similar open field test with four mice placed in four directions, mice showed similar behavioral responses to those with mirrors. These results indicate that mice are able to recognize images in the mirror, however, they cannot distinguish their own mirror images from the mirror images of other mice. To repeat the experiments of itch empathy, we compared the itch responses of mice in the mirrored environment, to those without. No significant difference in itching responses was detected. Differently, in the case of chemical pain (formalin injection), animals’ nociceptive responses to formalin during Phase II were significantly enhanced in the mirrored open field. A new format of heat map was developed to help the analysis of the trace of mice in the open field. Our results suggest that mice do recognize the presence of mice in the mirror, and their nociceptive - but not itch - responses are enhanced.
“Something Fun to Look Forward to”: Lessons From Implementing the Prescription for Health Farmers’ Market Initiative in Rural Upper Michigan

Fruit and vegetable (FV) prescription programs are an increasingly popular community-based approach to addressing food insecurity and improving nutrition by connecting local health care and food systems. The Prescription for Health farmers’ market FV prescription program was piloted in a rural, low-access low-income Michigan community in 2017. The program enrolled 33 adult participants with chronic disease and provided weekly farmers’ market FV vouchers, educational nutrition handouts, and seasonal healthy recipes over 10 weeks. Weight, blood pressure, and the following self-rated variables were assessed pre- and post program: dietary habits, food literacy, physical health, and mental health. While most metrics remained generally unchanged, one of the strongest findings from our data included significant improvement in quality of life. Increased social interaction as a result of the attending the farmers’ market was a prominent theme from informal open-ended participant feedback. Given the ongoing public health crisis of loneliness and social isolation, this finding led us to consider the farmers’ market as an avenue for creating opportunities for meaningful social connection among participants and farmers. To this end, we discuss health outcomes of the Prescription for Health pilot program, reflect on unique aspects of implementing this program in a rural area, and explore future opportunities for farmers’ market prescription programs as an innovative form of nature-based social prescribing.
Influence of different irrigation methods on the alfalfa rhizosphere soil fungal communities in an arid region

Xinjiang is the largest arid and saline agricultural region in China. The common irrigation methods in this area are traditional flood irrigation and drip irrigation. In this study, we investigated the effects of these two irrigation methods on the fungal diversity, community structures, and functions in alfalfa rhizosphere soil as well as the associated environmental factors in northern Tianshan Mountain (Xinjiang, China). Soil enzyme activities (urease and neutral phosphatase) were significantly higher in the drip-irrigated alfalfa rhizosphere soil than in the flood-irrigated alfalfa rhizosphere soil, whereas the fungal alpha diversity in the drip-irrigated alfalfa rhizosphere soil was significantly lower than that in the flood-irrigated alfalfa rhizosphere soil. Six dominant fungal phyla were identified (>0.1%), with Ascomycota being the most abundant in all soils, followed by Basidiomycota (5.47%), Mortierellomycota (1.07%), Glomeromycota (0.55%), Rozellomycota (0.27%), and Chytridiomycota (0.14%). Ascomycota and Glomeromycota species were significantly less abundant in drip-irrigated alfalfa rhizosphere soil than in flood-irrigated alfalfa rhizosphere soil. A LEFSe analysis identified Cladosporiaceae (20.8%) species as the most abundant marker fungi in drip-irrigated alfalfa rhizosphere soil. Of the 13 fungal functional groups identified on the basis of the functional annotation using the FUNGuild database, Ectomycorrhizal (22.29%) was the primary functional group. Compared with flood irrigation, drip irrigation significantly decreased the relative abundance of Ectomycorrhizal and Arbuscular_Mycorrhizal, while increasing the relative abundance of Plant_Pathogen, although not significantly (P = 0.19). Available potassium was revealed to be the main environmental factor influencing soil enzyme activities, fungal alpha diversity, fungal community structures, and fungal functions in response to the different irrigation methods. In conclusion, drip irrigation may be more appropriate than flood irrigation in the Tianshan dryland agricultural area for enhancing soil enzyme activities, but it may also increase the abundance of plant pathogenic fungi in the soil.
The Role of Insulin-like Growth Factor-Axis and Mitotic Index in South Indian Neonates with Small for Gestational Age

Abstract Objective IGF-axis and mitotic capacity of cells play a vital role in fetal growth. We compared IGF1, IGF2, and IGFBP3 protein levels, mitotic indices, IGFR1 and IGFR2 mRNA expression in appropriate for gestational age (AGA) and small for gestational age (SGA) neonates of Indian women. Methods Cord blood (n = 80) and placental samples (n = 40) were collected from AGA and SGA neonates. Plasma IGF1, IGF2, and IGFBP3 proteins were measured by ELISA. IGFR1 and IGFR2 mRNA expression in the placenta were analyzed by qRT-PCR. Cord blood was cultured in vitro and mitotic index was obtained. Results IGF1 (p = 1), and IGF2 (p = 0.69) protein levels did not differ, whereas IGFBP3 (p = 0.02) was significantly less in SGA compared to AGA neonates. Down-regulation of IGFR1 (3.9-folds) and IGFR2 (2.8-folds) mRNA and reduced mitotic index of lymphocytes was observed in SGA (p = 0.001) compared to AGA neonates. Conclusion Our results showed that, SGA neonates displayed down-regulated IGFR1 and IGFR2 mRNA, decreased IGFBP3 protein and mitotic index.
Droughts and societal change: The environmental context for the emergence of Islam in late Antique Arabia

In Arabia, the first half of the sixth century CE was marked by the demise of Himyar, the dominant power in Arabia until 525 CE. Important social and political changes followed, which promoted the disintegration of the major Arabian polities. Here, we present hydroclimate records from around Southern Arabia, including a new high-resolution stalagmite record from northern Oman. These records clearly indicate unprecedented droughts during the sixth century CE, with the most severe aridity persisting between ~500 and 530 CE. We suggest that such droughts undermined the resilience of Himyar and thereby contributed to the societal changes from which Islam emerged. Description Arabian aridity Islam emerged in the Arabian Peninsula in the early 7th century CE after a period of profound societal changes. What was the environmental context of that emergence? Fleitmann et al. present a hydroclimate record of Southern Arabia showing that droughts plagued the region throughout the 6th century CE. They suggest that increasing aridity and declining agricultural yields may have contributed to demise of Himyar, the dominant power in Arabia at that time. —HJS Persistent droughts in Arabia were coincident with profound societal changes there during the 6th century CE.
Applying Stress and Coping Theory to Understand Diuretic Adherence Experiences in Persons with Heart Failure

Despite the benefits of diuretics for treating the symptoms of heart failure (HF), patients may report side effects and intentionally not adhere to diuretic regimens. Positive internal motivators, such as positive emotions, may benefit individuals in their adaptation to medication-related stress. However, there has been limited study of these potential motivators in those with HF. Using a descriptive qualitative approach, 82 adults taking diuretics for HF were interviewed. This study applied stress and coping theory to understand the diuretic-taking experiences of patients with HF. Data analysis revealed three themes: (a) diuretics are bothersome, (b) staying positive in the mid of hardship, and (c) adapting to endure. Findings suggest that adherent participants stayed positive amid the perceived hardship, maintaining resilient and grateful attitudes. Adherent participants adapted to bothersome diuretic effects and utilized creative strategies. More research is needed to understand the relationships between resilience, adaptive coping, and diuretic adherence.
Electronic Absorption Spectroscopy and Photochemistry of Criegee Intermediates

Interest in Criegee intermediates (CIs), often termed carbonyl oxides, and their role in tropospheric chemistry has grown massively since the demonstration of laboratory‐based routes to their formation and characterization in the gas phase. This article reviews current knowledge regarding the electronic spectroscopy of atmospherically relevant CIs like CH2OO, CH3CHOO, (CH3)2COO and larger CIs like methyl vinyl ketone oxide and methacrolein oxide that are formed in the ozonolysis of isoprene, and of selected conjugated carbene‐derived CIs of interest in the synthetic chemistry community. Of the aforementioned atmospherically relevant CIs, all except CH2OO and (CH3)2COO exist in different conformers which, under tropospheric conditions, can display strikingly different thermal loss rates via unimolecular and bimolecular processes. Calculated photolysis rates based on their absorption properties suggest that solar photolysis will rarely be a significant contributor to the total loss rate for any CI under tropospheric conditions. Nonetheless, there is ever‐growing interest in the absorption cross sections and primary photochemistry of CIs following excitation to the strongly absorbing 1ππ* state, and how this varies with CI, with conformer and with excitation wavelength. The later part of this review surveys the photochemical data reported to date, including a range of studies that demonstrate prompt photo‐induced fission of the terminal O–O bond, and speculates about possible alternate decay processes that could occur following non‐adiabatic coupling to, and dissociation from, highly internally excited levels of the electronic ground state of a CI.