Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of Immune Effects Between Brucella Recombinant Omp10-Omp28-L7/L12 Proteins Expressed in Eukaryotic and Prokaryotic Systems

Brucella, a genus of bacteria that causes brucellosis, infects and threatens domestic animals, and humans in endemic areas. Presently, some live attenuated vaccines of Brucella are used to immunize livestock; however, these vaccines are pathogenic to humans, can provoke abortion when administered to pregnant livestock, and induce antibodies in vaccinated livestock that affect the diagnosis of field infection. It is, therefore, very important for improving the safety and immune protection effects of Brucella vaccine. Currently, recombinant protein-based subunit vaccines are considered promising safe and effective alternatives against brucellosis. Here, we separately expressed the recombinant Omp10-Omp28-L7/L12 proteins of Brucella using eukaryotic and prokaryotic expression systems, which were then used as immunogens for evaluating their immune responses. Taishan Pinus massoniana pollen polysaccharides (TPPPS), an already verified natural adjuvant, was utilized to evaluate the immune conditioning effect on the recombinant proteins. Antibody levels, spleen lymphocyte proliferation, percentages of CD4+ and CD8+ T cells, and cytokine secretion in mice were examined after three successive immunizations. The protective effects against Brucella challenge were also evaluated in mice, and used a live vaccine as a positive control. The results indicated that the immune responses of the recombinant Omp10-Omp28-L7/L12 protein groups were significantly higher than those of the PBS control group. The recombinant Omp10-Omp28-L7/L12 protein expressed in Pichia pastoris (P. pastoris) exhibited a slightly higher expression level and immunogenicity than that expressed in Escherichia coli (E. coli), and the Omp10-Omp28-L7/L12 (P. pastoris) + TPPPS group provided the most pronounced immune effect. The protective results showed that the recombinant Omp10-Omp28-L7/L12 proteins expressed in the two expression systems had significantly better protective effects against Brucella melitensis challenge compared with the negative control, and the addition of TPPPS adjuvant could significantly improve the protective effects of subunit vaccines. However, we also noticed that all of the evaluated subunit vaccines induced less protection than the B. melitensis M5 live vaccine. These results indicate that the combination of recombinant Omp10-Omp28-L7/L12 antigen and TPPPS adjuvant shows potential as an effective brucellosis subunit vaccine, and P. pastoris is a preferred expression system to prepare this recombinant subunit antigen.
Demographic projections of the Apennine brown bear population Ursus arctos marsicanus (Mammalia: Ursidae) under alternative management scenarios

Abstract Apennine brown bears are a very small, isolated population of central Italy, consisting of about 50 individuals and under a severe risk of extinction. We performed a population viability analysis (PVA) for this population, contrasting a deterministic model and an individual-based stochastic model, using a set of demographic parameters estimated for the same population during the last decade. We also built a set of simulated management scenarios, in which we compared the effectiveness of alternative conservation measures and assessed the susceptibility of the population to catastrophic mortality events. The deterministic model produced an estimate of the asymptotic population growth rate r = 0.001, corresponding to an asymptotically stable population. The stochastic model produced an estimate of r = − 0.013 (standard deviation = 0.103), corresponding to an annual population decrease of 1.3%, a 17% extinction risk in 100 years, an average population of 27 bears for non-extinct populations, and an average time to extinction of 81 years for those gone extinct. Extinction probability increased to more alarming levels (> 0.4) when at least one catastrophic event occurred during a 100-year period. Current vital rates of the population are not compatible with a more than negligible numerical increase, and this bear population is likely to remain small and exposed to a relatively high risk of extinction, if the average survival or reproductive rates do not increase. Management efforts aimed to increase food availability generated minimal to moderate variations in population growth rate and in the associated risk of extinction, whereas interventions meant to reduce adult female mortality were highly effective in increasing persistence probability. We propose that the general objectives of the action plan for the conservation of the Apennine brown bear for the incoming decade should explicitly contemplate quantitative demographic goals, focusing in particular on adult female and cub mortality.
“Donate to help combat COVID-19!” How typeface affects the effectiveness of CSR marketing?

Corporate social responsibility (CSR) marketing has become ubiquitous in the hospitality industry. The purpose of this paper is to examine the effectiveness of donation appeals containing warmth-focused versus competence-focused messages in hospitality CSR marketing. Moreover, we offer an innovative visual design strategy focusing on the typeface (handwritten vs machine-written) in donation appeals to encourage consumers’ donations and boost their brand loyalty. ,This research used a 2 (message framing: warmth-focused vs competence-focused) × 2 (typeface: handwritten vs machine-written) between-subjects experimental design. ,The findings suggest that donation appeals featuring warmth-focused messages combined with handwritten typeface and competence-focused messages combined with machine-written typeface can maximize donation intention and brand loyalty. Furthermore, results from the moderated mediation analyses indicate that brand trust is the psychological mechanism underlying these effects. ,Hospitality managers should use typeface design, which is easy and inexpensive to manipulate, to enhance the effectiveness of CSR marketing. Specifically, for donation appeals featuring warmth-focused (competence-focused) messages, the handwritten (machine-written) typeface can boost consumers’ donation intention and brand loyalty. ,To the best of the authors’ knowledge, this research is the first to reveal the competitive advantage of typeface design in hospitality CSR marketing. This research sheds light on the congruency effects of message framing and typeface design in donation appeals on consumers’ donation intention and brand loyalty while using the contemporary context of The Coronavirus Disease 2019 to test the theory.
Tectonic setting of Cretaceous porphyry copper deposits of northern Chile (28°-30° S) and its relations with magmatic evolution and metallogeny

Cretaceous porphyry copper deposits of northern Chile (28º-29º30’ S) are genetically related with dacitic to dioritic porphyries and they represent a still poorly-explored target for Cu resources. The porphyries correspond to stocks distributed into two separated discontinuous NS trending belts of different age. The location of these porphyries is generally adjacent to orogen-parallel major fault systems that extend along the studied segment and also have a marked temporal relationship with deformation events registered along these structures. A first episode of Cu-bearing porphyry emplacement took place between 116 and 104 Ma (Mina Unión or Frontera, Cachiyuyo, Punta Colorada, Dos Amigos, Tricolor porphyries). These Early Cretaceous dacite to diorite porphyries are spatially associated with the eastern segments of the Atacama Fault System, which records sinistral transpression that started at 121 Ma producing ground uplift, consequent denudation and exhumation of the Early Cretaceous magmatic arc. This resulted in a change from marine to continental deposition with an angular unconformity in the site of the back-arc basin after of eastward migration of the deformation around 112-110 Ma. At the scale of the continental margin, this deformation is correlated with early stage of the Mochica Orogenic event described in Perú. A second episode of Cu-bearing porphyry emplacement occurred between 92 and 87 Ma (Elisa, Johana, Las Campanas and La Verde deposits), which are spatially and temporally associated with the regional-scale Las Cañas-El Torito reverse fault, active between 89 and 84 Ma, during the Peruvian Orogenic Phase. This fault up thrust to the west part of the Chañarcillo Group rocks (Lower Cretaceous) over the younger upper levels of the Cerrillos Formation (Upper Cretaceous). The integrated geological mapping and geochemical data of the Early to Late Cretaceous volcanic rocks indicates that both Early Cretaceous sinistral transpression and Late Cretaceous east-west compression were not significant in promote changes in magma genesis, except for slight changes in trace element ratios (increase in Th/Ta, Nb/Ta and La/Yb) suggesting that the Late Cretaceous deformation event produced only slightly increase in crustal thickness (>40 km), but far from being comparable to major Cenozoic orogenic phases, at least along the magmatic arc to back-arc domains in the study area. Finally, our study give insights about regional geological parameters that can be used as a first order guide for exploration of Cu resources along Cretaceous magmatic belts of northern Chile, where both Early and Late Cretaceous Cu-bearing porphyry intrusions are restricted to a large structural block bounded to the west and east by Cretaceous fault systems.
Chromosomal distribution of rRNA genes in the karyotypes of two dioicous liverwort species from the genus Pellia Raddi

Over one hundred years have passed since the first cytogenetic studies were made on the liverwort genus Pellia Raddi. The karyotype of Pellia is characterised by large chromosomes, a varying heterochromatin content and the presence of sex chromosomes in the dioicous species. Most of the Pellia species are diploids with n = 9, but one of them, Pellia borealis Lorb. , has been described as an example of allopolyploidy in liverworts. Although the localisation of rRNA genes, which are essential components of the nuclear genome, remains a challenge in bryophytes, data on the number and chromosomal localisation of 35S and 5S rDNA in all of the Pellia species are now available. Previously, fluorescence in situ hybridisation using rDNA probes was performed on the mitotic chromosomes of 2 monoicous species. The aim of this study was to establish the number and chromosomal distribution of rRNA genes in 2 dioicous diploid species—Pellia endiviifolia (Dicks.) Dumort. and Pellia neesiana (Gottsche) Limpr. The relationships between the species within the genus Pellia can now be discussed in the context of the localisation of the rDNA sites and the range in the number of rDNA loci among bryophytes can also be verified.
Upgrading Manual Turning Machine Towards IoT-Based Manufacturing

Technological independence is one aspect of the challenges of the industrial revolution era 4.0. Digitalization, robotization, big data, internet of things (IoT), and efficiency of work processes require an update in technology. If everything is held in the fulfillment of practicum facilities, it will undoubtedly cost a prohibitive fee. Many of the tools and machines found in learning machining practices in Vocational High Schools (VHS) in Indonesia are still conventional machine. This study provides a breakthrough solution in the effectiveness and efficiency of traditional machining of the lathe that starts with a preliminary identification of literature, selection according to inclusion and exclusion criteria, coding, themes classifications, and supplementary areas. Through the literature review methodology, this article has the main objective of identifying the needs in architecture and the need for supporting devices on conventional lathes based on the Internet of Things (IoT) as well as safe, comfortable, and modern work areas in lathe machining practices.
Improving live body weight gain of local sheep through crossbreeding with high yielding exotic Dorper sheep under smallholder farmers

The study was conducted in Hawzen and Hintalowagerat districts of Tigray region Northern Ethiopia with the objective of enhancing meat production performance of the local sheep through cross breeding with high yielding exotic Dorper sheep under smallholder farmers. Pure male Dorper mated with local sheep produced first and second generation with 50 and 25% blood level of Dorper, respectively. Farmer’s research groups were established in both locations. The average live body weight of the 50% crossbred male and female sheep was 45 and 38 kg, respectively at adult age. The average live body weight of the adult male and female local sheep (highland) was 25 and 20 kg, respectively. On farm crossbreeding of Dorper (male) sheep with local sheep (female) improved body weight by 55% (male) and 53% (female) at yearly age. Smallholder farmers perceived that Dorper crossbred sheep are appropriate for meat production improvement. It is concluded that regardless of the black body color and the appearance short thin tail of the crossbred, Doper sheep breed is the appropriate technology for enhancing meat production performance of the local sheep. Key words: Crossbreeding, Dorper sheep, meat production, local sheep.
The Effect of Problem-based Learning and Mathematical Problem Posing in Improving Student’s Critical Thinking Skills

This study aims to determine whether there was differences effect in improving student’s critical thinking skills (1) between students who get Problem-based Learning, Mathematical Problem Posing, and conventional learning, and (2) based on gender differences. This study used a quantitative method with the pre-test-posttest two treatment design. Data were collected using instruments: test, documentation, and observation. Data was taken from 124 undergraduate students from the mathematics education study program at Universitas Negeri Semarang, Indonesia. Data analysis was done by using the normalized gain and the MannWhitney test. The results showed that in improving student’s critical thinking skills (1) Problem-based Learning and Mathematical Problem Posing provide better effect than conventional learning, (2) there was no significant difference in effect between Problem-based Learning and Mathematical Problem Posing, and (3) there was no significant difference in effect based on gender differences.
Fungal volatiles influence plant defence against above‐ground and below‐ground herbivory

Plants have evolved resistance traits that negatively affect attackers, and tolerance traits that sustain plant growth despite herbivore damage. These mechanisms often co-occur in a mixed-defence strategy, balancing resistance and tolerance. These plant defences can be enhanced upon interaction with soil micro-organisms. Here we investigated the effects of volatiles emitted by soil-borne fungi on plant defence to insect herbivory, and on plant phenology. We exposed roots of Brassica rapa plants to volatiles emitted by four soil-borne fungi. As a proxy of plant resistance, we assessed the performance of Pieris brassicae, a caterpillar feeding on leaves and inflorescences, and of Delia radicum, an insect root herbivore. As a proxy of plant tolerance, we compared growth of volatile-exposed plants challenged with or without insects. Additionally, we assessed the effects on plant phenology by recording bolting time and by counting the number of buds and flowers. Plant exposure to fungal volatiles differentially affected plant resistance to above- and below-ground herbivory. Performance of P. brassicae caterpillars differed between the fungal volatile-exposed plants but was variable between experimental batches. In contrast, the effects of fungal volatiles on D. radicum performance were predominantly negative, indicating an increased plant resistance. Despite root consumption by D. radicum, root dry weight remained unchanged in infested plants compared with uninfested ones, irrespectively of the volatile exposure, suggesting compensation for the tissue loss, sometimes at the cost of undamaged above-ground tissues. When B. rapa plants were attacked by P. brassicae caterpillars, only exposure to volatiles of some fungi led to compensation for the loss of above-ground tissues consumed by the caterpillars, which differed between leaves and inflorescences. Furthermore, bolting was accelerated in response to volatiles of some fungi, resulting in more buds and flowers, which suggests a potential enhancement of plant fitness. Our data show that fungal volatiles can modulate the mixed-defence strategies of B. rapa plants, balancing plant resistance and tolerance to above- and below-ground herbivory. These effects may be variable and were fungus specific. Ultimately, plant fitness may be enhanced upon root exposure to fungal volatiles. A free Plain Language Summary can be found within the Supporting Information of this article.
Exploring Teacher Attrition in Urban China through Interplay of Wages and Well-being

Teacher attrition is a chronic challenge facing many education systems, and has been shown to negatively impact education quality and equity. Common explanations rooted in occupational choice theory identify pecuniary and non-pecuniary rewards as critical factors in motivating and retaining teachers. Using China Household Income Project (CHIP) urban dataset, which contains detailed information on teacher career decisions, this study examines these theoretical stipulations by simultaneously modeling teacher career decisions, wage compensation, and on-the-job well-being. Probit panel regression results demonstrate that a 10% increase in teacher wage and well-being gaps, relative to comparable professionals, predicts a 7.9% and 32% lower probability of retention respectively, with effects mostly operating through teacher well-being.
Summers of war. Affective volunteer tourism to former war sites in Europe

Abstract An important aspect of contemporary volunteer tourism is generated by the possibility of having personal, emotional and affective encounters and experiences. Volunteer summer camps on former war sites, organized by the German Action Reconciliation Service for Peace (ARSP), can be regarded as an expression of a contemporary form of tourism, which consists of the development of a personal, affective, and immersive approach to learning and volunteering on former war sites. A performative approach to studying emotion is applied, and helps to locate and understand the social, cultural, and political components that instigate the desire for affective volunteer tourism. For this study, 26 semi-structured in-depth interviews have been conducted with participants of ARSP volunteer summer camps that focused on conserving and maintaining former war sites in Italy, Lithuania, and France. The results indicate that volunteers expect war themed summer camps to be impactful (in terms of work) and emotional (in terms of personal experiences). Yet, the sought-after impact and emotion are not always found, which gives rise to contradictory feelings and tensions. Feelings of guilt about unmet expectations have caused volunteers to re-evaluate their motives and look for different ways to make the summer camps meaningful to them. Participants were encouraged to critically reflect, on this form of volunteer tourism in particular, and on societal debates about war and volunteer tourism in general.
Pyrolysis Conversion of Polymer Wastes to Noble Fuels in Conditions of the Slovak Republic

This paper deals with the pyrolysis conversion of synthetic waste materials into noble fuels, i.e., heating oils, gasoline, diesel, and carbon. The following article presents the principle and use of pyrolysis conversion of waste tires and plastics. The core of the paper is the determination of energy properties of noble fuels obtained from pyrolysis conversion and the possibility of their real use in industry. The aim of this paper is a technical-economic evaluation of the use of waste pyrolysis in practice in the Slovak Republic. Unlike various methods of waste management, there are also more efficient methods, which primarily have a positive effect on the ecology of our Earth and at the same time can be effectively used for the production of alternative fuels. One of these methods is the pyrolysis conversion of synthetic waste materials into noble fuels. It is an ecological, waste-free, economical, and economical disposal of waste with a full recovery of its energy and material components with reduced emissions, and therefore this direction of using synthetic waste for the conversion of alternative fuels contributes to sustainable development. A significant advantage of this waste management is considered to be the fact that only waste tires or chlorine-free plastics are used as input materials without other necessary raw materials obtained by other economic activity. Tires and plastics are generated daily as waste in every household.
Penerapan Kajian dan Praktik Lapangan Pada Mahasiswa Pendidikan Olahraga di Universitas Negeri Malang (Mata Kuliah: Pembelajaran Tenis Meja dan Metodologi Penelitian)

Tujuan dari penelitian deskriptif ini adalah mengungkap fenomena yang terjadi secara kualitatif selama kegiatan kajian dan praktik lapangan (KPL) berlangsung dengan memberikan materi perkuliahan kepada mahasiswa S1 jurusan pendidikan jasmani dan kesehatan (PJK) di Fakultas Ilmu Keolahragaan (FIK) Universitas Negeri Malang (UM) dengan mengajar mata kuliah pembelajaran tenis meja (perwakilan mata kuliah praktik) dan metodologi penelitian (perwakilan mata kuliah teori). Metode deskriptif kualitatif digunakan, dengan peneliti sendiri sebagai instrumen pengumpulan data kemudian data dianalisis dengan: (1) data reduction, (2) data display, dan (3) verification. Hasil menunjukkan selama KPL berlangsung pada mata kuliah praktik dan teori dapat berjalan dengan baik meskipun terdapat beberapa hambatan. Hambatan yang terjadi secara umum pada mata kuliah praktik adalah mahasiswa kadang kurang terkontrol, sedangkan pada mata kuliah teori penyusunan tugas masih belum memenuhi harapan. Kesimpulan yang dapat ditarik adalah KPL berjalan dengan baik dan dapat memberikan pengalaman untuk mengatasi permasalahan yang terjadi selama memberikan perkuliahan.
Challenges in predicting Greenland supraglacial lake drainages at
the regional scale

A leading hypothesis for the mechanism of fast supraglacial lake drainages is that transient extensional stresses briefly allow crevassing in otherwise compressional ice flow regimes. Lake water can then hydrofracture a crevasse to the base of the ice sheet, and river inputs can maintain this connection as a moulin. If future ice sheet models are to accurately represent moulins, we must understand their formation processes, timescales, and locations. Here, we use remote-sensing velocity products to constrain the relationship between strain rates and lake drainages across ∼ 1600 km2 in Pâkitsoq, western Greenland, between 2002– 2019. We find significantly more extensional background strain rates at moulins associated with fast-draining lakes than at slow-draining or non-draining lake moulins. We test whether moulins in more extensional background settings drain their lakes earlier, but we find insignificant correlation. To investigate the frequency at which strain-rate transients are associated with fast lake drainage, we examined Landsat-derived strain rates over 16 and 32 d periods at moulins associated with 240 fast-lake-drainage events over 18 years. A low signal-to-noise ratio, the presence of water, and the multi-week repeat cycle obscured any resolution of the hypothesized transient strain rates. Our results support the hypothesis that transient strain rates drive fast lake drainages. However, the current generation of ice sheet velocity products, even when stacked across hundreds of fast lake drainages, cannot resolve these transients. Thus, observational progress in understanding lake drainage initiation will rely on field-based tools such as GPS networks and photogrammetry.