Miracle-dz/UniFAR-FTUs huggingface dataset:
Goji berry fruit extracts induce cytotoxicity and apoptotic cell death in breast cancer cells

: Anti-cancer agents derived from dietary medicinal plants are of great interest among people. The fruits of the Goji berry ( Lycium barbarum L., Fam. Solanaceae), also known as the ‘King of the Berries’, have valuable health benefits and pharmacological properties thanks to their rich content of phytochemicals. Considering the wide range of biological and pharmacological properties of goji berries, it was aimed to investigate the anticancer, antiproliferative and apoptotic cell death activities against human breast adenocarcinoma and carcinoma cells, MCF-7 and T47D, respectively. In the present study, the anticancer activities of the extracts were evaluated using MTT assay, the antiproliferative effects were investigated using bromodeoxyuridine (BrdU) cell proliferation assay, and the apoptotic cell death activities of the extracts were analyzed by immunological-based ELISA. As the main result of this research, it was found that extracts of goji berries significantly decreased the cell viability of breast cancer cells and caused these cells to undergo apoptosis-mediated cell death in a dose-and time-dependent manner. Among the tested extracts, methanol extracts showed the highest anticancer activity against MCF-7 and T47D cells (IC 50 =54.06±0.05 µ g/mL and 76.14±0.38 µ g/mL, p<0.01, respectively), while the lowest activity was observed in goji berry extracts prepared with dichloromethane (IC 50 =101.05±0.14 µ g/mL, p<0.05 and 124.10±0.86 µ g/mL, p<0.01, respectively). Moreover, the methanol extracts caused the strongest antiproliferative activity in MCF-7 cells (p<0.05). Regarding the apoptotic cell death potential of the extracts, increased apoptotic cell death was observed in both breast cancer cells, however, apoptotic cell death occurred more strongly in MCF-7 cells than in T47D cells. Consequently, this study suggests that goji berries could be valuable natural sources for the development of herbal formulations against breast cancer. Accordingly, further detailed studies should be conducted to elucidate the molecular signaling pathways and mechanisms of action.
Unexpected mapping of recurrent laryngeal nerve by fluorescence-guided surgery using near-infrared indocyanine green angiography

Background. The recurrent laryngeal nerve (RLN) damage and parathyroid gland injury are the most severe complications of thyroid surgery. The possibility of RLN confirmation in the near-infrared spectrum after the injection of indocyanine green (ICG) was not yet been studied. Aim: to evaluate the ICG angiography for the identification of RLN during thyroid and parathyroid surgery. Materials and methods. ICG angiography of RLN was performed in 7 patients. An intraoperative neuromonitoring was applied as a method of controlling RLN. During the operation, parathyroid glands and RLN were identified by visual inspection (naked eye). To further confirm the location of the parathyroid glands by autofluorescence, an intravenous injection of ICG was performed with a concentration of 0.25 mg/kg followed by the application of the image-based system. Results. A good signal was achieved in the near-infrared spectrum from the RLN in all cases after the ICG injection. Sufficient blood perfusion of the RLN could be considered as a reasonable explanation for the exhibition of a good ICG near-infrared signal. Conclusions. ICG use might be a helpful approach for the confirmation of the RLN in addition to routine visual identification. Such function could be applied during fluorescence-guided surgery to evaluate the parathyroid gland autofluorescence. Visualization of RLN by ICG angiography is considered as an additional useful feature to prevent RLN injury.
The Association Between Higher Expression of Talin-1 and the Reduced Survival Rate in Ovarian Serous Carcinoma Patients

Background & Objective: Talin-1 is a constituent of the multiprotein adhesion complexes that play main role in the formation of tumors and migration in different types of malignancies. The present study aimed to assess expression and prognostic significance of the talin-1 protein in ovarian serous carcinoma (OSC) patients. Methods: The expression of talin-1 in mRNA and its protein levels were investigated for ovarian cancer (OC) by using bioinformatics tools, including Gene Expression Profiling Interactive Analysis 2 (GEPIA2), Gene Expression Database of Normal and Tumor Tissue 2 (GENT2), and The University of ALabama at Birmingham CANcer data analysis Portal (UALCAN) databases. Thereafter, immunohistochemical (IHC) staining was used to study the expression patterns of the talin-1 protein using 46 paraffin-embedded OSC tissue specimens, 25 benign tumors, and 20 normal tissues, which were assembled in tissue microarrays (TMAs). We also assessed the potential association between the expression of the talin-1 protein, various clinicopathological parameters, and survival outcomes. Results: Our IHC examination for talin-1 was significantly overexpressed in OSC tissues compared to benign tumors and normal tissues. The Kaplan-Meier survival analysis has also indicated statistically significant differences in terms of disease-specific survival (DSS) and progression-free survival (PFS) between the patients with high and low expression levels of talin-1, respectively. Conclusion: The talin-1 protein was overexpressed in OSC tissues, and a high expression level of talin-1 was found to be significantly associated with tumor aggressiveness and poorer DSS or PFS. Therefore, talin-1 may serve as a molecular marker of cancer progression and a novel prognostic biomarker in these patients.
Comparative Transition System Semantics for Cause-Respecting Reversible Prime Event Structures

Reversible computing is a new paradigm that has emerged recently and extends the traditional forwards-only computing mode with the ability to execute in backwards, so that computation can run in reverse as easily as in forward. Two approaches to developing transition system (automaton-like) semantics for event structure models are distinguished in the literature. In the first case, states are considered as configurations (sets of already executed events), and transitions between states are built by starting from the initial configuration and repeatedly adding executable events. In the second approach, states are understood as residuals (model fragments that have not yet been executed), and transitions are constructed by starting from the given event structure as the initial state and deleting already executed (and conflicting) parts thereof during execution. The present paper focuses on an investigation of how the two approaches are interrelated for the model of prime event structures extended with cause-respecting reversibility. The bisimilarity of the resulting transition systems is proved, taking into account step semantics of the model under consideration.
Evaluation of Liver Enzymes in Normal Pregnancies in a University Hospital of Zabol Iran: A Cross-Sectional Study

Objective: Changes in endocrine, nervous, renal, cardiovascular, and respiratory systems during pregnancy have been studied, but changes in liver function have been poorly studied. Therefore, the purpose of this study was to investigate the trend of changes in liver enzymes in normal pregnancy. Materials and methods: This prospective longitudinal study included 68 pregnant women who were referred to the Obstetrics and Gynecology Clinic of Amiral Momenin Hospital in Zabol in 2021. In terms of the trimester of pregnancy, the presence of underlying diseases, history of previous pregnancies, disorders of the enzymes of recent patients, the patients were evaluated, and the information from the patients' files was analyzed. Results: The average AST levels in pregnant women in the first, second, and third trimesters were 16.82, 17.47, and 18.00, respectively, which show that garlic consumption is increasing. The average PT in pregnant women decreased in the first, second, and third trimesters. The average direct and total bilirubin levels in pregnant women in the first and second trimesters showed a constant trend. The amount of total protein increased in pregnant women during the first, second, and third trimesters. In the second and third trimesters, the enzyme level was significantly higher in pregnant women than in nonpregnant women. The level of GGT in pregnant women in the first, second, and third trimesters showed a different trend. Conclusion: Accurate evaluation of patients, especially in the third trimester, is necessary from the point of view of increasing enzyme levels in other countries.
Unit Weight and Elastic Modulus of Concrete utilizing Volcanic Stone Waste as Coarse Aggregate

Unit weight and elastic modulus of concrete utilizing volcanic stone waste (VSW) as coarse aggregate have been studied at 28 days of hydration. The origin of VSW was the residual of volcanic stone processing for making the handmade ornaments of traditional Balinese buildings. Three types of concrete mixture compositions were made by varying the proportion of cement, fine aggregate and coarse aggregate in concrete. Each mixture composition stirred using four variations of water/cement (w/c) ratio. For each variation in w/c, five cylindrical specimens with a diameter of 150 mm and a height of 300 mm were made to measure the unit weight and modulus of elasticity of concrete specimens after 28 days of hydration. The result shows that the unit weight and the elastic modulus of concrete decrease with increasing w/c at fixed proportions of cement and aggregates and increase with increasing proportion of aggregates at fixed w/c. The increase in elastic modulus with an increase in the a/c ratio for a fixed w/c ratio is more noticeable for a w/c ratio of more than 0.6. Moreover, the elastic modulus increases correspondingly with an increase in unit weight for a given a/c ratio.
Use of benthos in lake monitoring

The paper discusses the rationale behind the use of biological variables in environmental monitoring of lakes, as well as the principles of variable selection and the limitations of data usability. Profundal benthic communities are suggested to be an integral measure of autotrophic and heterotrophic lake processes. Measures of community structure and their relationship to morphometric and edaphic factors are presented and discussed, including indicator species/communities, diversity/species richness, oligochaete/chironomid ratio, and oligochaete abundance.
Influence of Low-Level Jet intensity on aerodynamic loads of horizontal axis wind turbine rotor

ABSTRACT High wind speeds associated with Low-Level Jet (LLJ) make wind resources more favorable for wind energy production. However, the aerodynamic loads of large-scale horizontal axis wind turbine (HAWT) rotor under different LLJ inflow conditions have not been thoroughly studied. To gain insight into the aerodynamic loads of rotor under LLJ inflow conditions with different LLJ intensities, a method to establish an engineering LLJ inflow model was proposed according to the plane wall jet theory and Von Karman spectra model with user-defined scaling. The parameters in the engineering LLJ inflow model were determined by comparing the wind speed distribution obtained from the GP_LLJ spectral model, which was summarized from field measurements in the real atmosphere. The LLJ fluctuating wind fields with different intensities generated by the engineering LLJ inflow model were used as the inflow conditions of Fatigue, Aerodynamics, Structures, and Turbulence (FAST) open source code to calculate and analyze the aerodynamic loads of the HAWT. It was found that the engineering LLJ inflow model can be used to establish the LLJ inflow condition of HAWT. When the LLJ height is located at the hub height and LLJ intensity increases from 8 to 16 m/s, the RMS rotor unbalanced aerodynamic load coefficients, including ones of lateral force, longitudinal force, tilt moment and yaw moment are increased by 2.2, 2.13, 1.02 and 0.95 times, respectively.
Decrease of Graft Failure Rate by Improving Tactics and Surgical Techniques in Extraanatomic Bypass Operations

We analized the early and late postoperative complications that occurred after 693 bypass operations (574 anatomic and 119 extra-anatomic) performed in 10 consecutive years (1997-2006). The bypass graft failures (infection + thrombosis) treatment and the subsequent evolution of the patients were detected by analyzing patients’ readmissions made to solve these major post-bypass complications. The follow-up period extended up to 12 years after the primary arterial bypass reconstruction. In this period we registered: 89 secondary arterial reconstructions for early and late graft thrombosis; 8 healings after removal of the infected prostheses, followed by extraanatomic bypass operations (7 obturator bypass + 1 axillo-bifemoral bypass). The most frequent vascular prosthesis infection sites were the Scarpa triangle and the thoraco-abdominal subcutaneous segment of the axillo(bi)-femoral graft. Major amputations after extraanatomic bypass operations: at 4 years postoperatively for axillo(bi)-femoral operations the amputation rate was 17.6%, while for crossover operations it was 7.5%. Perioperatory mortality after anatomic bypass operations = 2 intraoperatory and 8 postoperatory (1.38%/574 operations); perioperatory mortality after extraanatomic bypass operations: 2 postoperatory deaths (1.68%/119 operations), 1 after axillo-femoral prosthesis infection and 1 after acute myocardial infarction. In order to reduce the graft major complication rate after extraanatomic bypass operations we took the following measures: 1. When there are arterial occlusive lesions distal to the femoral tripod we ensured an adequate outflow which favores the long-term patency of the extraanatomic graft; this goal was obtained by 2 methods: by enlargement profundoplasty and/or by performing an additional distal bypass towards the popliteal artery or towards the subgenicular arteries. The distal subgenicular anastomosis was made between a venous graft and the distal outflow artery for reducing the intimal hyperplasia. 2. For crossover bypass operations we often used autologous vein grafts; 3. In crossover ilio-femoral bypass operation with venous graft we avoid the graft compression in its path through abdominal wall by passing the graft through a stable caliber hole made in a polypropylene closure mesh of the abdominal wall. 4. We promote the tunneling of the axillo-femoral prosthesis through the subaponeurotic muscular tissue because this tissue better protects the prosthetic graft against infection. 5. In order to reduce the groin prosthesis infections, we prefer performing ilio-femoral crossover bypass instead of femoro-femoral one. 6. The obturator bypass remains a valuable tratment for groin infected prosthesis.
The Potential of Promotional Media as A Marketing Instrument for Open and Distance Higher Education: A Segmentation Study

This research identifies market segments among university students based on media promotion usage. It examines the impact of media promotions on the choice of programs and institutions of open and distance university institutions. This study used a quantitative methodology, and the population studied consisted of all currently enrolled Universitas Terbuka students throughout Indonesia, with a total sample of 2,250 people. In this research, market segmentation has been carried out using the cluster method, which includes demographic, geographic, psychographic, and behavioral segments. The demographic segment that is assessed age and gender of students, recent graduates, and income; The geographic segment is mapped to the origin of the UT regional offices; the psychographic segment includes reasons for studying and choosing UT; and the behavioral segment from which information is first and most effective. This research implies that the results can be used as a reference in supporting higher education promotion. The Novelty of this study is that no other studies have compared the potential of promotional media as a marketing instrument for open and distance higher education.
DIVERSITY OF BUTTERFLY AND ANGEL FISHES ASSEMBLAGES AROUND ZABARGAD ISLAND, RED SEA, EGYPT -

Diversity of two groups of reef fish (butterfly fishes and angel fishes) assemblages was investigated around Zabargad Island, Red Sea. Zabargad Island (St. John's Island) is on the border of northern central part in the remote south of Egypt, 70 km off the coast. The island is almost 300 m above the sea level. Four sites were investigated around the Island. Underwater visual census techniques had been used to record fish abundances on reefs. Species richness was expressed by considering the number of species (D) and species diversity. Homogeneity were determined using the Shannon-Wiener diversity index (H') and the evenness index (J'). The total number of butterflyfish species recorded, at different sites, was 10 species belonging to two genera. There are two obvious patterns of distribution of butterfly fishes with depth around Zabargad Island. The highest average abundance was also recorded at site 1 (22 fish/600 m3), with the lowest value at site 2 (10 fish/600 m3). The total number of angelfish species recorded, at different sites, was 7 species belonging to five genera. All angelfish species are not found on the reef flat (except Pygoplites diacanthus and Centropyge multispinis) but they preferred the reef slope and increasing with depths. Substrate composition was calculated as percentage cover for all taxa. Several hard and soft coral genera were recorded from the study sites. On the reef flat, dead components, soft corals and marine plants (especially algae) were dominated, which represented by 42.83%, 31.38%, and 12.25%, respectively. On the reef wall or slope, hard corals, dead components and soft coral were dominated, and represented by 47.55%, 23.83%, and 16.70%, respectively. Linear regression equations were calculated to investigate the relationships between fish community and benthic composition of the substrate.
Determinants of Use Behavior in Utilizing Fintech Investment Management for Gen-Z

This study aims to analyze behavioral intention and use behavior in using fintech investment management through the Unified Theory of Acceptance and Use of Technology 2 (UTAUT 2) model, namely performance expectancy, effort expectancy, social influences, facilitating conditions, hedonic motivation, price values and habits. This study used quantitative approach. The population in this study were all Generation Z in Bali which determine using convenience sampling method. The data analysis technique in this research is multiple regression using Smart-pls software. The results of the analysis provide evidence that performance expectancy, social influences, facilitating conditions, hedonic motivation and habits had positive effect on behavioral intention in using fintech investment management. In addition, behavioral intention had a positive effect on the use behavior of using fintech investment management.
Effects of irrigation, effluent dispersal and organic farming on earthworms and soil microbes in New Zealand dairy farms

New Zealand pastoral farming is undergoing intensification by conversion of sheep/beef farms to dairy, often supported by intensive irrigation. Dairy effluent dispersal is an important farming practice that raises efficient milk production and reduces pollution of waterways. This study measured how conversion, irrigation and effluent dispersal affect earthworm species composition, distribution, density, age structure and biomass by sampling earthworms across six treatments: (i) Dairy pastures with added effluent, (ii) with added water through irrigation, (iii) effluent and water added together, and (iv) neither water nor effluent were added; and sheep/beef pastures (v) without irrigation, and (iv) with irrigation. Altogether 615 Soil Monitoring Sites were sampled in 205 paddocks and 41 farms in the Waimate District of New Zealand in winter and spring 2012. Four earthworm species were observed, but Aporrectodea caliginosa dominated (≈70% of individuals, ≈65% of biomass). Their density on dairy farms increased by 42% from untreated to effluent only treatments; and by further 95% where irrigated. Maximum density occurred where both effluent dispersal and irrigation occurs. Lumbricus rubellus (22.6% of individuals, 26.8% of biomass) increased by 32% where effluent had been spread on dairy paddocks, by 123% if the paddock was irrigated, and by 180% where both effluent and irrigation water was added together. Higher density and biomass in irrigated treatments was associated with a much higher proportion of juveniles in the L. rubellus population. Aporrectode longa was rare (5.9% of individuals, 5.2% of biomass) and appears to be driven locally extinct in many irrigated soils. The strong experimental effects observed for all species between treatments were evident in the top 10 cm of soil only. Octolasion cyaneum was also recorded occasionally. Earthworm density was 15.4% to 36.6% and biomass was -3.3% to 55.8% higher on sheep/beef farms than dairy farms when equivalent treatments were compared. Many factors influence earthworm communities, but irrigation and effluent dispersal have strong over-riding effects that boost epigeic and endogeic earthworm abundance, probably by enhancing habitat, reducing risks of desiccation and providing extra food in the top 10 cm of soil. The comparative lack of anecic worms in
Neurological Manifestation in Hospitalized Patients With Acute SARS-CoV-2 Infection

Objective: The main objective of this research is to determine the prevalence and characteristics of neurological manifestations in hospitalized patients with SARS-CoV-2 infection. Methods: A cross-sectional study was conducted. 572 hospitalized patients at the COVID Department of Pulmonology of the Mostar University Clinical Hospital in the six-month period from October 31, 2020, to April 30, 2021, were included. We analyzed the incidence of neurological manifestations and the influence of comorbidities and metabolic syndrome on stroke incidence in COVID-19 patients. We analyzed hospital length of stay and mortality in patients with and without neurological manifestations. The research was conducted with respect to all the determinants of the Helsinki Declaration. Results: 572 patients, 351 men (61.4%), and 221 women (38.6%) were included. A fatal outcome was present in a quarter of patients (25.3%). Neurological manifestations were found in 163 patients (28.5%). Myalgia was the most common (16.1%). The following were reported: headache (9.6%), loss of taste (7.34%), loss of smell (6.8%), and vertigo (2.5%). There was a significant difference regarding loss of smell between males and females (p=0.04). The cerebrovascular incident was present in 2.97% of patients and was more frequent in the group of patients with metabolic syndrome. Patients with neurological manifestations had a longer hospital stay, but it was not statistically significant (p=0.9319). The presence of neurological manifestations in general did not influence the mortality rate. Conclusion: Patients with SARS-CoV-2 infection can present with neurologic findings such as myalgia, headache, loss of smell or taste, vertigo, as well as cerebrovascular incidents. Patients with neurological manifestations had longer hospital stays, but the presence of neurological manifestations in general did not influence the mortality rate.