Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic parameters for meat quality and carcass traits and their correlation with wool traits in Australian Merino sheep

Estimates of genetic parameters for carcass traits in Merino sheep are presented, including muscle pH and colour, which are indicators of meat quality. The inclusion of these in the parameter set for genetic evaluation will provide the basis for more accurate estimated breeding values and the design of better breeding programs for improving meat traits in straightbred Merino sheep and crossbred lamb production. Data from 1045, nineteen-month-old Merino rams, the progeny of 125 sires from 3 strains that are representative of the industry, were used. Heritability and genetic correlations were estimated by fitting an animal model using a REML procedure in univariate and multivariate analyses. The traits included fasted pre-slaughter liveweight; dressing %; carcass tissue depth at the GR site (FATGR); fat depth at the C site (FATC); eye muscle depth (EMD), width (EMW), and area (EMA); ultimate pH and colour (L*, a*, b*) of the eye muscle. Multivariate analyses were used to estimate genetic correlations among meat traits and between wool traits (clean wool weight and mean fibre diameter) and selected carcass and meat traits. Estimates of heritability were: liveweight 0.51 (±0.10), dressing % 0.39 (±0.10), FATGR 0.33 (±0.09), FATC 0.20 (±0.08), EMD 0.27 (±0.08), EMW 0.15 (±0.07), EMA 0.23 (±0.08), muscle pH 0.27 (±0.09), and 0.14 (±0.07), 0.02 (±0.06), and 0.04 (±0.06) for the meat colour measurements L*, a*, and b* respectively. There was a favourable genetic correlation between muscle pH and colour L* of –0.56 (±0.23). There were also significant genetic correlations between wool weight and FATGR (–0.34 ± 0.16) and between mean fibre diameter and muscle colour L* (–0.66 ± 0.27). The study indicates that there is genetic variation for carcass traits, including indicators of meat quality, with scope for selection to improve meat production and quality in Merino sheep.
Genotype–Phenotype Correlation in Children: The Impact of FBN1 Variants on Pediatric Marfan Care

Currently, no reliable genotype–phenotype correlation is available for pediatric Marfan patients in everyday clinical practice. We investigated correlations of FBN1 variants with the prevalence and age of onset of Marfan manifestations in childhood and differentiated three groups: missense/in-frame, splice, and nonsense/frameshift variants. In addition, we differentiated missense variants destroying or generating a cysteine (cys-missense) and alterations not affecting cysteine. We categorized 105 FBN1-positive pediatric patients. Patients with cys-missense more frequently developed aortic dilatation (p = 0.03) requiring medication (p = 0.003), tricuspid valve prolapse (p = 0.03), and earlier onset of myopia (p = 0.02) than those with other missense variants. Missense variants correlated with a higher prevalence of ectopia lentis (p = 0.002) and earlier onset of pulmonary artery dilatation (p = 0.03) than nonsense/frameshift, and dural ectasia was more common in the latter (p = 0.005). Pectus excavatum (p = 0.007) appeared more often in patients with splice compared with missense/in-frame variants, while hernia (p = 0.04) appeared earlier in the latter. Findings on genotype–phenotype correlations in Marfan-affected children can improve interdisciplinary therapy. In patients with cys-missense variants, early medical treatment of aortic dilatation seems reasonable and early regular ophthalmologic follow-up essential. Patients with nonsense/frameshift and splice variants require early involvement of orthopedic specialists to support the growing child.
Comparing associations between frailty and mortality in hospitalised older adults with or without COVID-19 infection: a retrospective observational study using electronic health records

Abstract Background The aim of this study was to describe outcomes in hospitalised older people with different levels of frailty and COVID-19 infection. Methods We undertook a single-centre, retrospective cohort study examining COVID-19-related mortality using electronic health records, for older people (65 and over) with frailty, hospitalised with or without COVID-19 infection. Baseline covariates included demographics, early warning scores, Charlson Comorbidity Indices and frailty (Clinical Frailty Scale, CFS), linked to COVID-19 status. Findings We analysed outcomes on 1,071 patients with COVID-19 test results (285 (27%) were positive for COVID-19). The mean age at ED arrival was 79.7 and 49.4% were female. All-cause mortality (by 30 days) rose from 9 (not frail) to 33% (severely frail) in the COVID-negative cohort but was around 60% for all frailty categories in the COVID-positive cohort. In adjusted analyses, the hazard ratio for death in those with COVID-19 compared to those without COVID-19 was 7.3 (95% CI: 3.00, 18.0) with age, comorbidities and illness severity making small additional contributions. Interpretation In this study, frailty measured using the CFS appeared to make little incremental contribution to the hazard of dying in older people hospitalised with COVID-19 infection; illness severity and comorbidity had a modest association with the overall adjusted hazard of death, whereas confirmed COVID-19 infection dominated, with a sevenfold hazard for death.
Seasonal Variation in Highly Unsaturated Fatty Acid Composition of Muscle Tissue of Two Fishes Endemic to the Eastern Mediterranean

Fatty acid content and profile in muscle tissue of two commercially important fishes from the Eastern Mediterranean were analyzed. One fish, Siganus rivulatus or rabbitfish, is an herbivore while the other fish, Diplodus sargus or white sea-bream, is a carnivore. Our aim was to evaluate changes in health benefits to consumers of said fishes among seasons and among species with different diets. Total fat content of the muscle in both fishes was low, being highest in August for the rabbitfish (3.31g per 100 g wet tissue) and in October for the white sea-bream (2.27 g per 100 g). Omega-3 and omega-6 highly unsaturated fatty acids (HUFA) as a proportion of total lipids also varied with season in both fishes. The total amount of lipid consumed per weight of tissue varied monthly among the species and one species is not necessarily better than another all year. However, on average, a person that consumes only rabbitfish throughout the year will eat more marine lipid than a consumer that eats only white sea-bream. Nonetheless, both species studied supply essential ω-3 and ω-6 HUFA. The ω-6:ω-3 ratio in fish in the present work was generally less than 2.0, but a diet that includes these fishes supplemented with some vegetable oil would raise the ratio to the recommended value of 4 to 6.
Evaluation of arsenate- and vanadate-associated changes of electrical membrane potential and phosphate transport in Lemna gibba G1

Interference of arsenate and vanadate with phosphate uptake in Lemna gibba L. was studied by measuring voltage changes and (32P)phosphate uptake. Arsenate proved to be competitive with the highand low-affinity phosphate uptake system. It induced transient membrane potential changes of up to 120 mV which were similar to those induced by phosphate and indicated a cotransport mechanism with at least 2H+/H2As04. The amplitude of the transient arsenate-induced membrane depolarization was strongly influenced by phosphate starvation. A permanent membrane depolarization to the diffusion potential was achieved within 2 to 6 h in P-starved plants. Thus, arsenate is indeed a strongly competitive physiological analogue of phosphate in higher plants. Vanadate was easily transported into L. gibba as concluded from transient Em changes of up to 110 mV. Vanadate interfered only slightly and non-specifically with the two phosphate transport mechanisms. Like phosphate, vanadate uptake seems to be an H+-cotransport mechanism, both with similar optima at pH 6 0. Unlike phosphate uptake, vanadate-linked membrane depolarization was not affected by high intracellular phosphate concentrations. P-starvation did not enhance the weak long-term effect on Em. Hence, vanadate, in contrast to arsenate, is not regarded as a physiological phosphate analogue. The distinct and rapid vanadate-induced and permanent membrane depolarization of Avena sativa, Triticum aestivum and Glycine max leaves was not seen in Lemna nor in leaves of Gossypium hirsutum and Nicotiana tabacum. Plasmalemma-enriched preparations of L. gibba revealed, however, a high vanadate-sensitive ATPase activity (87%). As a possible explanation for these differences it is suggested that the latter plant species have cytosolic vanadate-detoxifying properties, i.e. they can reduce vanadate to vanadyl ions, in contrast to the former group of plant species.
A Biodegradable Antifungal-Loaded Sol–Gel Coating for the Prevention and Local Treatment of Yeast Prosthetic-Joint Infections

Fungal prosthetic-joint infections are rare but devastating complications following arthroplasty. These infections are highly recurrent and expose the patient to the development of candidemia, which has high mortality rates. Patients with this condition are often immunocompromised and present several comorbidities, and thus pose a challenge for diagnosis and treatment. The most frequently isolated organisms in these infections are Candida albicans and Candida parapsilosis, pathogens that initiate the infection by developing a biofilm on the implant surface. In this study, a novel hybrid organo–inorganic sol–gel coating was developed from a mixture of organopolysiloxanes and organophosphite, to which different concentrations of fluconazole or anidulafungin were added. Then, the capacity of these coatings to prevent biofilm formation and treat mature biofilms produced by reference and clinical strains of C. albicans and C. Parapsilosis was evaluated. Anidulafungin-loaded sol–gel coatings were more effective in preventing C. albicans biofilm formation, while fluconazole-loaded sol–gel prevented C. parapsilosis biofilm formation more effectively. Treatment with unloaded sol–gel was sufficient to reduce C. albicans biofilms, and the sol–gels loaded with fluconazole or anidulafungin slightly enhanced this effect. In contrast, unloaded coatings stimulated C. parapsilosis biofilm formation, and loading with fluconazole reduced these biofilms by up to 99%. In conclusion, these coatings represent a novel therapeutic approach with potential clinical use to prevent and treat fungal prosthetic-joint infections.
Distribution of Zooplankton from Arabian Sea, along Southern Kerala (Southwest Coast of India) During the Cruise

Among different groups of zooplankton copepods contributed maximum numerical abundance contributed up to (31.93%). 26 species of copepods constituted by 20 species of calanoida, 3 species of harpatocoida and 3 species of cyclopoida. The predominant species of copepods were Acartia spinicuda, Calanus finmarchicus, Paracalanus parvus, Acrocalanus gracilis, and Euterpina acutiferons. Less number of Ichthyoplankton encountered during present study is an indication of anthropogenic hydrographical changes in coastal waters and its adverse impact on fishery potential of these transects. Salinity showed a significant positive correlation with zooplankton density. Relatively low zooplankton density in the near shore stations compared to offshore may be attributed to salinity variations and marginal stress from the fresh water input. Thus the present attempt is made to study the distribution of zooplankton recorded in Arabian Sea along Southern Kerala. Among the stations, the highest zooplankton population of 366 Nom^(-3) was recorded at Neendakara 5km offshore and Veli near shore (EDP) recorded the lowest density of 60 Nom^(-3) The minimum zooplankton density encountered at Veli transect might be due to constant exposure to precipitate, severe drop in pH, low dissolved oxygen leading to asphyxiation and death of large population.
Key Opportunities to Replace, Reduce, and Refine Regulatory Fish Acute Toxicity Tests

Fish acute toxicity tests are conducted as part of regulatory hazard identification and risk‐assessment packages for industrial chemicals and plant protection products. The aim of these tests is to determine the concentration which would be lethal to 50% of the animals treated. These tests are therefore associated with suffering in the test animals, and Organisation for Economic Co‐operation and Development test guideline 203 (fish, acute toxicity) studies are the most widely conducted regulatory vertebrate ecotoxicology tests for prospective chemical safety assessment. There is great scope to apply the 3Rs principles—the reduction, refinement, and replacement of animals—in this area of testing. An expert ecotoxicology working group, led by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research, including members from government, academia, and industry, reviewed global fish acute test data requirements for the major chemical sectors. The present study highlights ongoing initiatives and provides an overview of the key challenges and opportunities associated with replacing, reducing, and/or refining fish acute toxicity studies—without compromising environmental protection. Environ Toxicol Chem 2020;39:2076–2089. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Utilization of dissolved inorganic carbon (DIC) and the response of the marine flagellate Isochrysis galbana to carbon or nitrogen stress.

The growth of the marine flagellate Isochrysis galbana was followed in batch cultures at four concentrations of dissolved inorganic carbon (DIC), from C- and N-replete lag phase into C- and/or N-deplete stationary phase. Organic buffers were omitted from the growth medium, and culture pH was maintained at 8.30±0.05 by the addition of acid or alkali. The responses of the flagellate to N stress included an increase in the C∶N ratio, and decreases in the ratios of glutamine (Gln)∶glutamate (Glu) and Chl a∶C, and the cell Chl a quota. Conversely, the responses to C stress included a decrease in the C∶N ratio, and increases in the ratios of Gln∶Glu and Chl a∶C, and the cell Chl a quota. The relationship between carbon-specific growth rate (C-μ), and the concentration of extracellular DIC, [DIC]ext , exhibited Michaelis-Menten type kinetics with a half saturation constant, KG(DIC) , of 81 μM. Comparative studies of the diatom Phaeodactylum tricornutum showed similar results, although the value of KG(DIC) was lower at 30 μM.
Sequence matters – selective adaptation in electroantennographic response to binary odour mixtures by the Colorado potato beetle

Chemically mediated behaviour of insects is often strongly affected by mixtures of odour stimuli and their temporal characteristics. Both sensory transduction and central processing of odour mixtures can give rise to several different kinds of interaction, which can influence how individual components are perceived and processed. In particular, odour mixtures have been examined in model experiments as premixed binary mixtures in comparison with pure odour stimuli. Only in few experiments, the influence of the temporal structure of odour mixtures on odour perception has been taken into account. Natural odour stimuli often have a pulsed structure and may in general be superimposed on a background of irrelevant or interfering compounds, which can fluctuate with different frequencies, depending on their source. To achieve a better representation of these natural conditions, our odour mixing experiments apply a new kind of stimulation protocol: odours were not premixed but superimposed with a specific time pattern; one odour stimulus was presented as a longer persisting background and the second stimulus was a superimposed short test signal. To gain an overview of odour interaction patterns in the Colorado potato beetle by causing adaptation of one receptor population at naturally occurring levels of concentration and time intervals, electroantennographic recordings were made on excised antennae. A matrix of 12 stimulus compounds led to 132 pairs of compounds tested, each in the role of background and test stimulus. In 64 cases, the interaction was significantly different, when the role of background and stimulus was exchanged. Interaction patterns ranging from no interference (independence) to suppression were found and assigned to four clearly distinguishable types. We discuss that the observed effects of the presentation sequence in odour mixtures may contribute to the mechanisms of olfactory pattern recognition and olfactory contrast perception by insects.
Patient Satisfaction in the Peruvian Health Services: Validation and Application of the HEALTHQUAL Scale

During recent years, public–private partnerships (PPPs) in the health sector have been an attractive alternative for improving healthcare services in developing countries such as Peru. Therefore, it is fundamental to consider a comprehensive set of healthcare qualities, like the HEALTHQUAL scale, when we measure dimensions of healthcare service quality. Currently, no studies have applied HEALTHQUAL in Peruvian hospitals. The purposes of this study were to (1) validate and evaluate the application of the HEALTHQUAL scale to measure user satisfaction in outpatient services at two PPP hospitals in Peru; and (2) test the relationship between user satisfaction, efficiency, and loyalty. A descriptive, cross-sectional study based on the HEALTHQUAL scale was carried out at the end of 2018. The measurement items were satisfaction with healthcare personnel, satisfaction with nonhealthcare personnel, satisfaction with facilities and equipment, perception of efficiency, and trust. The scale was administered to a nonprobability sample of 250 users who attended one of two PPP hospitals—Barton and Kaelin. The application of partial least squares path modeling significantly impacted on the perceived efficiency in the items of healthcare personnel, nonhealthcare personnel, and facilities and equipment. The HEALTQUAL scale demonstrated sufficient validity and thus can be applied for measuring user satisfaction in PPP hospitals.
A European consensus statement on the use of four‐factor prothrombin complex concentrate for cardiac and non‐cardiac surgical patients

Modern four‐factor prothrombin complex concentrate was designed originally for rapid targeted replacement of the coagulation factors II, VII, IX and X. Dosing strategies for the approved indication of vitamin K antagonist‐related bleeding vary greatly. They include INR and bodyweight‐related protocols as well as fixed dose regimens. Particularly in the massively bleeding trauma and cardiac surgery patient, four‐factor prothrombin complex concentrate is used increasingly for haemostatic resuscitation. Members of the Transfusion and Haemostasis Subcommittee of the European Association of Cardiothoracic Anaesthesiology performed a systematic literature review on four‐factor prothrombin complex concentrate. The available evidence has been summarised for dosing, efficacy, drug safety and monitoring strategies in different scenarios. Whereas there is evidence for the efficacy of four‐factor prothrombin concentrate for a variety of bleeding scenarios, convincing safety data are clearly missing. In the massively bleeding patient with coagulopathy, our group recommends the administration of an initial bolus of 25 IU.kg‐1. This applies for: the acute reversal of vitamin K antagonist therapy; haemostatic resuscitation, particularly in trauma; and the reversal of direct oral anticoagulants when no specific antidote is available. In patients with a high risk for thromboembolic complications, e.g. cardiac surgery, the administration of an initial half‐dose bolus (12.5 IU.kg‐1) should be considered. A second bolus may be indicated if coagulopathy and microvascular bleeding persists and other reasons for bleeding are largely ruled out. Tissue‐factor‐activated, factor VII‐dependent and heparin insensitive point‐of‐care tests may be used for peri‐operative monitoring and guiding of prothrombin complex concentrate therapy.
Attraction of the grapevine moth to host and non‐host plant parts in the wind tunnel: effects of plant phenology, sex, and mating status

In the European grapevine moth, Lobesia botrana (Denis and Schiffermüller) (Lepidoptera: Tortricidae), it has recently been shown that volatiles emitted from the main host plant, grapevine, are attractive to adult females. Here, using wind tunnel experiments, we tested the attractiveness of various grapevine, Vitis vinifera L. (Vitaceae), plant parts at different phenological stages, including ripe berries infested with the pathogenic fungus Botrytis cinerea (Persoon: Fries) (Sclerotiniaceae) to laboratory‐reared virgin and mated male and female moths. We also tested the attractiveness of the non‐host plant, tansy [Tanacetum vulgare (L.) (Asteraceae)], as flowers and flower volatiles were previously shown to be attractive to L. botrana females in the field. Mated female moths were the only adults to exhibit upwind orientation to grape plant parts in the flight tunnel. The most attractive parts of the grapevine plants were leaves, flower buds, and ripe berries. No attraction was observed to flowers. This corresponds to the phenological stages available when females of the 2–3 generations during a year are present in the field. No attraction was observed to leaves and flowers of tansy.
Comparison of all suture fixation with tension band wiring and plate fixation of the olecranon

Background Tension band wiring and plate fixation are common techniques used to stabilize simple olecranon fractures and osteotomies of the olecranon. All suture fixation is an alternative technique but has not been compared previously to these traditional methods. The aim of this study was to compare the clinical and radiographic outcomes of the three techniques. Methods One hundred and sixty-eight consecutive Mayo type 1 and 2 olecranon fractures (n = 138) and olecranon osteotomies (n = 30) with a minimum follow-up time of one year were compared. The primary outcome measure was the rate of re-operation. Secondary outcome measures were the incidence of complications, rate of radiographic union and incidence of radiographic reduction loss. Results Fixation was performed using tension band wiring in 89 patients, plating in 38 patients and suture fixation in 41 patients. There was no significant difference in the fracture type according to the Mayo classification between the groups. The re-operation rate was significantly higher in the tension band wiring group (36%) compared with both the plate group (11%, p = 0.03) and the suture group (2%, p = 0.002). There were two revision fixations in the tension band wiring group and one in the suture group. There was one asymptomatic non-union in the suture group. All other fractures and osteotomies achieved radiographic union. Conclusion Suture fixation of simple olecranon fractures and osteotomies was reliable in providing stable union and had a significantly lower re-operation rate when compared with tension band wiring.