Miracle-dz/UniFAR-FTUs huggingface dataset:
Stability of DMPC Liposomes Externally Conjugated with Branched Polyglycerol

Vesicles formed by DMPC liposomes externally conjugated with branched polyglycerol-dendrons as well as linear PEG in water solution were simulated using the DPD method. Such a structure of vesicles corresponds to the structure of polymer-grafted liposomes obtained experimentally by the post-insertion method, in which polymer chains are fixed on the outer surface of the liposome. The grafting density, generation number and spacer length of grafted dendrons were varied. It was shown that modification of the outer surface of liposomes due to grafting of hydrophilic dendrons has practically no effect on the size and shape of the vesicle, as well as on the morphology of the lipid membrane up to certain critical thresholds of grafting density, degree of polymerization, and generation number of grafted molecules. Exceeding the threshold values of these structural parameters leads to irreversible deformation of the lipid membrane. Diffusion through the membrane and the transition of grafted molecules from the outer surface of the liposome to the inner surface is not observed for dendrons with a generation number higher than one, even at high grafting densities. The critical values of the generation number and the characteristics of the molecular coating at these values were determined for various grafting densities and spacer lengths of the grafted chains. It was shown that the chemical potential of the grafted dendron can serve as a stability metric for the conjugated liposome. The chemical potential of grafted molecules was calculated using the mean field model of the spherical brush on the liposome surface. An analysis of the simulation data shows that, within the framework of the applicability of the mean field approach, the value of the chemical potential is a sufficient criterion for separating vesicles into stable and unstable forms. These results can be used as a guide for the experimental design of nanocontainers based on lipid vesicles with an external protective coating of branched macromolecules.
Properties of Cephalopod Skin Ommochromes to Inhibit Free Radicals, and the Maillard Reaction and Retino-Protective Mechanisms in Cellular Models Concerning Oxidative Stress, Angiogenesis, and Inflammation

Ommochromes are pigments of invertebrates that exhibit oxidative stress protection. The aim of this study was to investigate ommochromes extracted from cephalopod’s skin for their ability to inhibit age-related-macular degeneration (AMD)-related factors such as H2O2-induced and iron-dependent oxidative stress (ferroptosis and erastin), accumulation of advanced glycation end-products (AGEs), as well as vascular endothelial growth factor (VEGF), and inflammatory cytokines (interleukin 6 and interleukin 8) secretion. As cell systems, we used primary porcine retinal pigment epithelium (RPE), human retinal pigment epithelium cell line ARPE-19 and uveal melanoma cell line OMM-1. In vitro, ommochromes produced an antiglycation effect by the inhibition of fructosylation reaction. The ommochromes showed protective effects against erastin- induced cell death in ARPE-19. In addition, in long-term stimulation (7 days) ommochromes decreased constitutively secreted VEGF, as well as interleukin 6 and interleukin 8 induced by Poly I:C in primary RPE. No relevant effects were detected in OMM-1 cells. The effects are dependent on the cell system, time of exposition, and concentration. This substance is of interest for further research concerning age-related macular degeneration.
Vaccinations Status against Vaccine-Preventable Diseases and Willingness to Be Vaccinated in an Italian Sample of Frail Subjects

Background: Study aim was to investigate the vaccination status against vaccine-preventable diseases (VPD) of frail adults during the SARS-CoV-2 pandemic and, for those subjects eligible for at least one vaccine, with respect to the recommended vaccination in line with the Italian National Vaccination Prevention Plane (NPVP), to explore the willingness to be vaccinated. Methods: A cross-sectional study was carried out among adults aged ≥ 60, immunocompromised or subjects affected by chronic conditions. Results: Among the 427 participants, a vaccination coverage rate lower than the targets for all the vaccines considered was found. Of those, 72.6% of subjects stated their willingness to receive recommended vaccinations, and 75.2% of the respondents stated that the advice to undergo vaccinations was received by the General Practitioner (GP). In a multivariable logistic regression model, higher odds of recommended VPD vaccination uptake (defined as having two or more of the recommended vaccinations) were associated with the willingness towards recommended VPD vaccination (Odds Ratio = 3.55, 95% Confidence Interval: 1.39 to 9.07), university education (OR = 2.03, 95% CI: 1.03 to 3.97), but having another person in the household (OR = 0.52, 95% CI: 0.28 to 0.97), and history of oncological disease (OR = 0.39, 95% CI: 0.18 to 0.87) were predictive of lower odds of vaccination uptake. In another multivariable model, higher odds of willingness to receive vaccines were associated with kidney disease (OR = 3.3, 95% CI: 1.01 to 10.5), perceived risk of VPD (OR = 1.9, 95% CI: 1.02 to 3.3), previous influenza vaccination (OR = 3.4, 95% CI: 1.8 to 6.5), and previous pneumococcal vaccination (OR = 3.1, 95% CI: 1.3 to 7.7), but increasing age (OR = 0.93 per year, 95% CI: 0.91 to 0.97), working (OR = 0.40, 95% CI: 0.20 to 0.78), and fear of vaccine side effects (OR = 0.38, 95% CI: 0.21 to 0.68) were predictive of lower odds of willingness to receive vaccines. Conclusions: Despite specific recommendations, vaccination coverage rates are far below international targets for frail subjects. Reducing missed opportunities for vaccination could be a useful strategy to increase vaccination coverage in frail patients during the routine checks performed by GPs and specialists.
Plausible Detection of Feasible Cave Networks Beneath Impact Melt Pits on the Moon Using the Grail Mission

In the future, when humans build their bases on terrestrial planets and their moons, caves will be the safest place for inhabitation. Large holes, believed to be cave entrances, have been discovered on the Moon, along with small features called “impact melt pits. ” In the Gravity Recovery and Interior Laboratory (GRAIL) gravity model, which is expressed in spherical harmonics (SH), it is difficult to express the gravity anomaly created by a small empty space below the surface. Nevertheless, we propose that a cave network, akin to an anthill, exists under the impact melt pits discovered on the Moon. This is because we think it is natural to apply a network created by Earth’s small caves to the Moon. We obtained accurate Bouguer gravity measurements by calculating regional crustal density using localized admittance of the study area and detected weak gravity (mass deficit) information. By increasing the degrees and order of SH at regular intervals, we estimated the change in gravity at a specific position at high degrees and order, thereby extracting shallow depth information. To validate our method, we compared our results with those of existing studies that analyzed the previously known Marius Hills Hole (MHH) area. The analysis of seven regions in our study area revealed a mass deficit in some impact melt pits in four lunar regions (Copernicus, King, Stevinus, and Tycho). We propose that there is a cave network in this region, indicated by the gravitation reduction in the impact melt pits region. Our results can be useful for the selection of landing sites for future in situ explorations of lunar caves.
Rhodojaponin III-Loaded Chitosan Derivatives-Modified Solid Lipid Nanoparticles for Multimodal Antinociceptive Effects in vivo

Background Rhodojaponin III (RJ-III) is a bioactive diterpenoid, which is mainly found in Rhododendron molle G. Don (Ericaceae), a potent analgesia in traditional Chinese medicine with several years of clinical applications in the country. However, its clinical use is limited by its acute toxicity and poor pharmacokinetic profiles. To reduce such limitations, the current study incorporated RJ-III into the colloidal drug delivery system of hydroxypropyl trimethyl ammonium chloride chitosan (HACC)-modified solid lipid nanoparticles (SLNs) to improve its sustained release and antinociceptive effects in vivo for oral delivery. Results The optimized RJ-III@HACC-SLNs were close to spherical, approximately 134 nm in size, and with a positive zeta potential. In vitro experiments showed that RJ-III@HACC-SLNs were stable in the simulated gastric fluid and had a prolonged release in PBS (pH = 6.8). Pharmacokinetic results showed that after intragastric administration in mice, the relative bioavailability of RJ-III@HACC-SLNs was 87.9%. Further, it was evident that the peak time, half-time, and mean retention time of RJ-III@HACC-SLNs were improved than RJ-III after the administration. In addition, pharmacodynamic studies revealed that RJ-III@HACC-SLNs markedly reduced the acetic acid, hot, and formalin-induced nociceptive responses in mice (P < 0.001), and notably increased the analgesic time (P < 0.01). Moreover, RJ-III@HACC-SLNs not only showed good biocompatibility with Caco-2 cells in vitro but its LD50 value was also increased by 1.8-fold as compared with that of RJ-III in vivo. Conclusion These results demonstrated that RJ-III@HACC-SLNs improved the pharmacokinetic characteristics of the RJ-III, thereby exhibiting toxicity-attenuating potential and antinociceptive enhancing properties. Consequently, HACC-SLNs loaded with RJ-III could become a promising oral formulation for pain management that deserves further investigation in the future.
Progressive Additive Benefits of Prehabilitation and Subsequent Bariatric Surgery on Cardiac Autonomic Regulation as Assessed by Means of a Simple Unitary Composite Index: Preliminary Data from an Observational Study

Obesity is associated with an increased risk of several chronic comorbidities, which may also be determined by dysfunctional autonomic nervous system (ANS). The influence of bariatric surgery (BS) on ANS balance was explored in previous studies, but with high heterogeneity in both the assessment timing and methods employed. In the present observational study, we applied a clinical protocol which considers two subsequent phases. Twenty-nine non-diabetic obese subjects were studied at baseline (T0), after one month of lifestyle modification (prehabilitation) (phase 1-T1), and after eight months following BS (phase 2-T2). ANS regulation was assessed across the three study epochs by means of ANSI, a single composite percent-ranked proxy of autonomic balance, being free of gender and age bias, economical and simple to apply in a clinical setting. The aim of the present study was to investigate the effects of the clinical protocol based on prehabilitation and subsequent BS on the ANS regulation by means of ANSI. Potential intertwined correlations with metabolic parameters were also investigated. Notably, we observed a progressive improvement in ANS control, even by employing ANSI. Moreover, the reduction in the markers of sympathetic overactivity was found to significantly correlate with the amelioration in some metabolic parameters (fasting glucose, insulin levels, and waist circumference), as well as in stress and tiredness perception. In conclusion, this study provides convincing evidence that a unitary proxy of cardiac autonomic regulation (CAR) may reflect the progressive improvement in autonomic regulation following behavioral and surgical interventions in obese patients. Intriguingly, this might contribute to reducing cardiovascular and metabolic risk.
Histopathological Evaluation of Annona muricata in TAA-Induced Liver Injury in Rats

This research in vivo assessed the impact of the ethanolic extract of Annona muricata (A. muricata) on the histopathology, immunohistochemistry, and biochemistry of thioacetamide (TAA)-induced liver cirrhosis in Sprague Dawley rats. The rats, gavaged precisely with two doses of A. muricata (250 mg/kg and 500 mg/kg) with TAA, presented a substantial reduction in the liver index and hepatocyte propagation, with much lower cell injury. These groups showed meaningfully down-regulated proliferating cell nuclear antigen (PCNA) in the liver and spleen, α-smooth muscle actin (α-SMA), and transforming growth factor-beta 1 (TGF-β1) in liver parenchymal tissue. The liver homogenate displayed enhanced antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) activity, along with a decrease in malondialdehyde (MDA) levels. The serum levels of bilirubin, total protein, albumin, and liver enzymes alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were returned to normal and were similar to that of the normal control and silymarin with TAA-treated groups. Oral acute toxicity revealed no evidence of any toxic symbols or mortality in rats, indicating the safety of A. muricata. Therefore, the normal microanatomy of hepatocytes, the clampdown of PCNA, α-SMA, TGF-β, improved antioxidant enzymes (SOD and CAT), and condensed MDA with repairs of liver biomarkers validate the hepatoprotective effect of A. muricata.
Role of IL-18 in Comparison to Serum Creatinine in Early Detection of Sepsis Induced Acute Kidney Injury in Emergency Department in Suez Canal University Hospital

Background: Septic acute kidney injury is a syndrome of acute impairment of function and organ damage linked with long-term adverse outcomes depending on the extent of acute injury superimposed on underlying organ reserve. Sepsis is the most important cause of Acute Kidney Injury (AKI). Interleukin-18 (IL-18) is a pro-inflammatory cytokine expressed in the renal cortex, peritubular capillaries, and interstitium. Aim: To assess the role of IL-18 in comparison to serum creatinine in the early detection of sepsis-induced acute kidney injury in the Emergency Department (ED) at Suez Canal University Hospital. Subjects and Methods: A comparative cross-sectional study that included two groups of participants. Study group: patient diagnosed with sepsis-induced acute kidney injury attending to the ED at Suez Canal University Hospital. Control group: healthy individuals of the same age group. Patients were clinically assessed and managed by the ABCDE protocol. All patients were subjected to Initial assessment including History, clinical examination, and laboratory investigation, including urinary IL-8. Results: Cases had statistically significant higher urinary IL-18 compared to controls (121.97 ± 75.84 vs 69.07 ± 35.59) (p<0.001). IL-18, a value of 69.5 IU/L was found to be the best cut-off point for the prediction of sepsis-induced AKI among cases, with sensitivity = 6 and 5% specificity = 57.5%. Conclusion: Urinary IL-18 can be used as an early predictor for AKI than serum creatinine in patients presenting with sepsis.
Observations of Electron Vorticity and Phase Space Holes in the Magnetopause Reconnection Separatrix

We report Magnetospheric Multiscale (MMS) observations of two electron vortices with a roughly 10 km or 2–3 electron skin depths cross‐section across the magnetospheric separatrix of a dayside magnetopause reconnection structure. The enhanced electron vorticity, which is caused by a significant velocity shear in the electron flows streaming in opposite directions along northward‐directed magnetic field lines, leads to perturbations perpendicular to the reconnecting magnetic field on the order of 1–2 nT. The high electron vorticity periods are also associated with electron phase space holes having the largest bipolar parallel electric field amplitudes ∼25 mV/m observed across this exhaust. Less intense ∼10 mV/m electron phase space holes propagate along a northward magnetic field away from the X‐line within a northward reconnection exhaust. The electron phase space holes with the largest bipolar parallel electric fields are observed to propagate toward the X‐line within discrete channels of southward and super‐Alfvénic 1,500 km/s electron flows in the magnetospheric separatrix layer.
ENERGY SPECTRA AND NUCLEAR INTERACTIONS OF COSMIC RAY PARTICLES

Results of investigations made at 3200 mn altitude during l953 to l956 are presented. The energy distri-butions of protons and mu mesons with energies up to lOO Bev were investigated. The measured proton and mu -meson spectra can be approximated respectively by thc expressions 3.2 x 10/sup -3/ (2 + E)sup -2.8/dE (for E > 3 Bev) and O.5 (5 plus or minus E)/sup -3/ dE (for E > 4 Bev). Data are presented for the cross sections for inelastic nuclear interaction of high-energy pi mesons and protons in graphite, copper, and lead. The proton and pi -meson inelastic nuclear interaction cross sections ( sigma / sub a/) were found to be equal. In graphite, copper, and lead, respectively, the following values were found: sigma /sub a/= 0.65 to 0.75 sigma /sub 0/, and 0.9 sigma /sub 0/,where sigma /sub 0/ = pi (1.4 x 10/sup -13/A/sup 1/3/)/sup 2/ is the geometrical nuclear cross section. (tr-auth)
RNAi-Mediated Silencing of Putative Halloween Gene Phantom Affects the Performance of Rice Striped Stem Borer, Chilo suppressalis

Simple Summary Ecdysteroids have a significant role in insect development and reproduction. Ecdysteroidogensis is regulated by a group of genes known as Halloween genes. We provide experimental evidence that Chilo suppressalis-phantom (Cs-Phm) in rice striped stem borer is a Halloween gene phantom and has important role in specific steps in ecdysteroidogensis. Three lines of experiments were done on typical protein domains of Cs-Phm, RNAi-mediated knockdown of Cs-Phm, and the rescue of larval development in dsRNA-treated groups. The results strongly suggest that Cs-Phm plays a critical role in ecdysteroidogensis in rice striped stem borer. Abstract The physiological and biochemical characterization of the “Halloween” genes has fundamental importance in the biosynthesis pathway of ecdysteroids. These genes were found to catalyze the final phases of ecdysteroid biosynthesis from dietary cholesterol to the molting hormone 20-hydroxyecdysone. We report the characterization of the Cs-Phm in a major insect pest in agriculture, the rice striped stem borer, Chilo suppressalis (C. suppressalis). A full-length transcript of Cs-Phm was amplified with an open reading frame (ORF) of 478 amino acids through 5′ and 3′ RACE. Cs-Phm shows five insect-conserved P450 motifs: Helix-C, Helix-I, Helix-K, PERF, and heme-binding motifs. Phylogenetic analysis clearly shows high similarity to Lepidoptera and evolutionary conservation in insects. The relative spatial and temporal transcript profile shows that Cs-Phm is highly expressed in the prothoracic glands and appears throughout the larval development, but with low expression at the start of the larval instar. It seems to peak in 3–4 days and decreases again before the larvae molt. Double-stranded RNA (dsRNA) injection of Cs-Phm at the larval stage efficiently knocked down the target gene and decreased its expression level. The dsRNA-treated group showed significantly decreased ecdysteroid titers, which leads to delayed larval development and higher larval mortality. Negative effects of larval development were rescued by treating 20E in the dsRNA-treated group. Thus, in conclusion, our results suggest that Cs-Phm is functionally conserved in C. suppressalis and encodes functional CYP that contributes to the biogenesis of 20E.
Who Punishes Leaders for Lying About the Use of Force? Evaluating The Microfoundations of Domestic Deception Costs

It is a common stereotype that leaders lie, but for all our beliefs about how normal it is for a president to lie to the public we know next to nothing about how voters might actually view this conduct. Drawing from literature in behavioral economics, we theorize that voters apply their attitudes towards interpersonal lies when judging leaders, with people who see lying as more socially acceptable being less willing to punish leaders for exhibiting this behavior. Using a novel vignette-style survey experiment, we find strong support for our theory: despite widespread willingness to punish leaders who lie, individuals high in Machiavellianism and Self-Monitoring are far less willing to punish presidents for lying than their low-type peers, and in some cases do not punish at all. Using robustness checks and causal mediation analysis, we confirm that views regarding the moral acceptability of lying drive responses to presidential deception.
Corporate governance and financial distress: An endogenous switching regression model approach in vietnam

Abstract This study aims to determine the impact of Corporate Governance on the relationship between the macro and micro factors causing financial distress in 240 Vietnamese listed non-financial firms. The study also investigates the marginal benefits of different corporate governance practices by applying an endogenous switching regression model (ESRM). The research clarifies that a firm with strong corporate governance practice has a low probability of financial distress compared to a weak corporate governance firm. Moreover, the risk of financial distress is significantly reduced when improving the corporate governance practice. This paper contributes empirical evidence on the predominant benefit of strong corporate governance practices and marginal benefit in risk mitigation in enhancing corporate governance. The article suggests that Vietnamese firms should implement strong corporate governance to overcome the risk of financial distress.
Openness to Experience Mediates the Relation Between Fantasy Proneness and Creative Thinking

Fantasy proneness has recently been related to creative thinking. To try and explain this link, we examined whether the relation was mediated by Openness to Experience (openness) because fantasy proneness and openness involve an imaginative thinking style. Study 1 assessed fantasy proneness (Creative Experiences Questionnaire), openness, and creative (divergent) thinking in 87 undergraduates (77% women, mean age 21 years). Study 2 replicated the method with museum visitors of similar age (58% women, mean age 23 years). Our hypotheses received partial support: although fantasy proneness did not directly predict creativity in either study, bivariate correlations in both studies revealed that fantasy proneness positively predicted openness, and openness positively predicted creativity. In addition, openness mediated the relation between fantasy proneness and creativity, but only in Study 2. These findings reveal potentially useful relations between fantasy proneness, openness, and creativity, and show that findings from student populations are not necessarily generalizable.
Personality Development Across Adolescence and Young Adulthood: The Role of Life Transitions and Self-Concept Clarity

Personality develops across the lifespan, but most development occurs in adolescence and young adulthood. Life transitions to new social roles may be important drivers of mean-level personality development. The present study examined mean-level personality development in adolescence and young adulthood, and the role of the transition to tertiary education and working life therein in a sample of Dutch young people that were followed across 14 years (N = 497, AgeW1 = 13.03 years). We explored whether young people’s self-concept clarity moderated these associations. Our hypotheses and analytical plan were pre-registered. Findings from Latent Growth Models showed support for maturation in personality across adolescence and young adulthood, but not a maturity dip. Having the role of employee was associated with higher conscientiousness, but no associations were found of the transition to tertiary education and the transition to work with mean-level development of any of the personality traits. Self-concept clarity did not moderate the role of transitions in mean-level personality development. Our findings suggest that socialization effects may not explain associations between life transitions and personality development in adolescence and young adulthood.