Miracle-dz/UniFAR-FTUs huggingface dataset:
Plasmodium Circumsporozoite Protein Enhances the Efficacy of Gefitinib in Lung Adenocarcinoma Cells by Inhibiting Autophagy via Proteasomal Degradation of LC3B

Background: Almost all lung adenocarcinoma (LUAD) patients with EGFR mutant will develop resistance to EGFR-TKIs, which limit the long-term clinical application of these agents. Accumulating evidence shows one of the main reasons for resistance to EGFR-TKIs is induction of autophagy in tumor cells. Our previous study found that circumsporozoite protein (CSP) in Plasmodium can suppress autophagy in host hepatocytes. However, it is unknown whether CSP-mediated inhibition of autophagy could improve the anti-tumor effect of EGFR-TKIs. Methods: We constructed A549 and H1975 cell lines with stable overexpression of CSP (OE-CSP cells). CCK-8, Lactate Dehydrogenase (LDH), flow cytometry, and colony analysis were performed to observe the effect of CSP overexpression on cell viability, apoptosis rate, and colony formation ratio. The sensitizing effect of CSP on gefitinib was evaluated in vivo using a subcutaneous tumor model in nude mice and immunohistochemical assay. The role of CSP in regulation of autophagy was investigated by laser confocal microscopy assay and western blotting. A transcriptome sequencing assay and real-time polymerase chain reaction were used to determine the levels of mRNA for autophagy-related proteins. Cycloheximide (CHX), MG132, TAK-243, and immunoprecipitation assays were used to detect and confirm proteasomal degradation of LC3B. Results: OE-CSP A549 and H1975 cells were more sensitive to gefitinib, demonstrating significant amounts of apoptosis and decreased viability. In the OE-CSP group, autophagy was significantly inhibited, and there was a decrease in LC3B protein after exposure to gefitinib. Cell viability and colony formed ability were recovered when OE-CSP cells were exposed to rapamycin. In nude mice with xenografts of LUAD cells, inhibition of autophagy by CSP resulted in suppression of cell growth, and more marked apoptosis during exposure to gefitinib. CSP promoted ubiquitin-proteasome degradation of LC3B, leading to inhibition of autophagy in LUAD cells after treatment with gefitinib. When LUAD cells were treated with ubiquitin activating enzyme inhibitor TAK-243, cell viability, apoptosis, and growth were comparable between the OE-CSP group and a control group both in vivo and in vitro. Conclusion: CSP can inhibit gefitinib-induced autophagy via proteasomal degradation of LC3B, which suggests that CSP could be used as an autophagy inhibitor to sensitize EGFR-TKIs.
Seismic Performance Evaluation of Two-story and Three-span Subway Station in Different Engineering Sites

ABSTRACT This study firstly investigates the seismic intensity measures (IMs) from the existing scalar ones that are suitable for the prediction of seismic response of shallowly buried rectangular underground structures. The peak acceleration and peak velocity at the ground surface are identified as the optimal IMs based on their efficiency, practicality, and proficiency. A series of seismic fragility curves are then developed for a two-story and three-span underground subway station embedded in three different typical engineering sites using nonlinear incremental dynamic analyses. In this study, the input ground motions at the level of engineering bedrock for the underground structure–soil interaction system are obtained through one-dimensional equivalent linear site response analysis according to the one-dimensional wave propagation theory. Comparison of the numerical results in this paper with the previous empirical and numerical fragility curves shows that the fragility analysis method in this paper is feasible, which can quantitatively give the failure probability of structures at different performance levels, and can provide reference for seismic design of underground structures. The numerical results indicate that both the characteristics of ground motions and the site profile have significant influence on the seismic fragility curves of underground structures. Underground subway stations are generally more vulnerable to seismic damage when embedded in engineering sites with lower average shear velocity. Moreover, seismic damage margin ratios are proposed for the underground structure in different site classes to show the confidence levels of its seismic performance when subjected to different levels of earthquake events, and can be used as a preliminary index for the earthquake risk assessment of subway station.
R&D and Innovation Collaboration between Universities and Business—A PLS-SEM Model for the Spanish Province of Huelva

In the last decade we have witnessed a growing amount of interest for developing better ‘exchange’ between universities, research centres and technology parks and companies, governments and other institutions. The biggest aim of those projects is, on the one hand, to make sure that valuable research does not stay hidden in the ivory tower of academia, and, on the other, that there are clear indications for what kinds of solutions are needed in the market. Due to the lack of empirical research in the topic, the focus of this paper is to establish and explain which factors determine the demand for technological services and how they can contribute to the promotion of greater university–business collaboration in R&D and innovation. To achieve that goal, we applied the PLS-SEM (Partial Least Squares Structural Equation Modelling) method in order to create a theoretical model, which was then verified through the application of the CTA (Confirmatory Tetrad Analysis) with the purpose of evaluating whether the specification of the chosen measurement model based on the theoretical rationale was supported by data. The test run was performed on 96 companies from the Spanish region of Huelva. It showed that only four of the considered factors, namely influence of the environment, market conditions, technology adoption decision and economic characteristics of the company, constituted 65.76% of the variance of the endogenous latent Demand for Technological Services. We believe that thanks to the proposed model and its adaptivity, it is possible to design relevant policies and undertakings aimed at promoting the research-business collaboration at the regional, national and international levels.
Quantifying and linking Sustainable Land-water-energy-food nexus: A perspective of sustainable livelihoods in Gidabo Watershed, East African Rift Valley

BackgroundThe sustainable management of Land - Water - Energy - Food (LWEF) nexus requires an environmental characterization that allows the comparison of complex interlinkages between nexus resources and livelihoods. This complexity makes this characterization difficult coupled with limited study in quantifying sustainability of LWEF nexus and its linkage with livelihood. Therefore, the present study aimed to investigate the link between sustainable LWEF nexus and livelihoods. We used analytical hierarchy process and pairwise comparison matrix in combination with weighting model.ResultThe result of composite LWEF nexus index was 0.083 representing, low sustainability. This could be linked with nexus resources consumption, use, and management. From the analysis of the weight of land, water, energy and food nexus resources, the highest weight was observed for food. The focus of on food production only shows no clear synergy on provisioning, supporting or regulating nexus resources to address livelihoods. The result further showed that LWEF nexus resources have strong correlation with livelihoods. This was evidenced by social (r ≥ 0.8, P < 0.01), natural (r ≥ 0.3, P < 0.05) and physical (r ≥ 0.6, P < 0.01) livelihood indicators showed strong positive correlation with LWEF nexus resources.ConclusionBased on the finding of the study, it was observed that managing nexus resources not only provide a significant contribution to achieve sustainable LWEF nexus, but also be effective for enhancing livelihood through food security. This could be attained by strong evidence based policy to ensure sustainable use of nexus resources. The results provided by this study would serve as the foundation for future study, policy formulation and implementation.
Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome

Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various spliceosomal RNA-protein complexes. In this review we give an overview of the crystal structures solved in the Nagai group, utilizing many of the strategies to design crystal packing as described in the accompanying paper.
Anammox-Based Processes for Mature Leachate Treatment in SBR: A Modelling Study

Mature landfill leachates are characterized by high levels of ammoniacal nitrogen which must be reduced for discharge in the sewer system and further treatment in municipal wastewater treatment plants. The use of anammox-based processes can allow for an efficient treatment of ammonium-rich leachates. In this work, two real scale sequencing batch reactors (SBRs), designed to initially perform partial nitritation/anammox (PN/A) and simultaneous partial nitrification and denitrification (SPND) for the treatment of ammonium-rich urban landfill leachate, were modelled using BioWin 6.0 in order to enable plant-wide modelling and optimizing. The constructed models were calibrated and validated using data from long- and short-term (one cycle) SBR operation and fit well to the main physical-chemical parameters (i.e., ammonium, nitrite and nitrate concentrations) measured during short-term (one cycle) operations. Despite the different strategies in terms of dissolved oxygen (DO) concentrations and aeration and mixing patterns applied for SBR operation, the models allowed for understanding that in both reactors the PN/A process was shown as the main contributor to nitrogen removal when the availability of organic carbon was low. Indeed, in both SBRs, the activity of nitrite oxidizing bacteria was inhibited due to high levels of free ammonia, whereas anammox bacteria were active due to the simultaneous presence of ammonium and nitrite and their ability to recover from DO inhibition. Increasing the external carbon addition, a prompt decrease of the anammox biomass was observed, with SPND becoming the main nitrogen removal mechanism. Models were also applied to estimate the production rates of nitrous oxide by aerobic ammonia oxidizing bacteria and heterotrophic denitrifiers. The models were found to be a robust tool for understanding the effects of different operating conditions (i.e, temperature, cycle phases, DO concentration, external carbon addition) on the nitrogen removal performances of the two reactors, assessing the contribution of the different bacterial groups involved.
Enhancing Researcher Reflexivity About the Influence of Leading Questions in Interviews

Interviewing is the most frequently used qualitative research method for gathering data. Although interviews vary across different epistemological perspectives, questions are central to all interviewing genres. This article focuses on the potential for the wording of interview questions to lead and unduly influence, or bias, the interviewee’s responses. This underacknowledged phenomenon affects the trustworthiness of findings and has implications for knowledge claims made by researchers, particularly in research that aims to elicit interviewees’ subjective experience. We highlight the problem of the influence of interview questions on data; provide a typology of how interview questions can lead responses; and present a method, the “cleanness rating,” that facilitates reflexivity by enabling researchers to review and assess the influence of their interview questions. This clarifies the researcher’s role in the production of interview data and contributes to methodological transparency.
Effect of Pyrolysis Reactions on Coal and Biomass Gasification Process

Thermodynamic analysis of a gasification process was conducted assuming that it is composed of two successive stages, namely: pyrolysis reaction followed by a stage of gasification reaction. This approach allows formulation the models of selected gasification processes dominating in industrial applications namely: Shell (coal), SES (coal), and DFB (dual fluid bed, biomass) gasification. It was shown that the enthalpy of fuel formation is essential for the correctness of computed results. The specific computational formula for a wide range of fuels enthalpy of formation was developed. The following categories were evaluated in terms of energy balance: total reaction enthalpy of gasification process, enthalpy of pyrolysis reaction, enthalpy of gasification reaction, heat demand for pyrolysis reaction, and heat demand for gasification reactions. The discussion of heat demand for particular stages of gasification related to the various processes was performed concluding the importance of the pyrolysis stage.
Multi-Level Fibrotomy for Pediatric Patients with Cerebral Palsy: A Cohort Study

Muscle retraction in Cerebral Palsy (CP) often requires surgical treatment. Multilevel procedures (using open or percutaneous techniques) are commonly performed in the ambulant patient with CP. The necessity to find new surgical techniques, reduce postoperative discomfort, and accelerate the healing process and rehabilitation is mandatory for these patients. A retrospective cohort study with 189 pediatric patients with CP was performed. The multilevel gradual fibrotomy of Ulzibat was modified using an ophthalmic knife. No significant complications were reported using our technique. Opioid drugs were not necessary, and casting time was reduced at the first 24 h. A significant Range of Motion recovery was assessed post-operatory and maintained at the last follow-up. Mean days of hospitalization were 2.2. The mean follow-up was 39 months (6–64 months). The modified multilevel fibrotomy reduces postoperative pain with easier patient management, resulting in a faster discharge from the hospital. However, the retrospective nature and the lack of a control group of the present study did not allow the authors to report significant results. Further studies with longer follow-up are in progress to obtain more certain data that confirm our preliminary results.
Exploring Chinese college students’ emotions as they engage in online learning during a pandemic

ABSTRACT This paper reports on the trajectory of college students’ emotions as they engaged in online learning during a pandemic and how their emotions related to their learning. Through a qualitative analysis of 11 college students’ reflections and interview data collected over one semester in a university in China, the case study shows the complexity of students’ emotions in the aforementioned context. The students’ emotions demonstrated a dynamic transition from anxiety and curiosity to emotional stability, and then back to anxiety, a phenomenon that interacted with the changing sources in the research context. The students also felt that their emotions while engaging in online learning interacted with their learning. Implications of the study include caring about and promoting students’ social-emotional well-being and encouraging students to regulate their emotions while learning online during a pandemic as well as in normal times.
A New 1‐Hourly ERA5‐Based Atmosphere De‐Aliasing Product for GRACE, GRACE‐FO, and Future Gravity Missions

The current state‐of‐the‐art of satellite gravity data processing makes use of de‐aliasing products to reduce high‐frequency mass anomalies. For example, the most recent official Atmosphere and Ocean De‐aliasing products (AOD1B‐RL06) are applied for the Gravity Recovery and Climate Experiment (GRACE) and GRACE‐Follow On (GRACE‐FO) missions. The temporal resolution of AOD1B‐RL06 is 3 h, and spectrally, they are computed up to degree and order 180. In this study, we explore a refined, e.g., geometrically, physically, and numerically improved, mass integration approach that is important for computing the atmosphere part of these products. Besides, the newly available ERA5 climate data are used to produce a new set of non‐tidal atmosphere de‐aliasing product (HUST‐ERA5) that is computed hourly up to degree and order 100, covering 2002 onwards. Despite an overall agreement with AOD1B‐RL06 (correlation ≥ 0.99), considerable discrepancies still exist between HUST‐ERA5 and AOD1B‐RL06. The possible reasons are therefore analyzed, and we find the input climate data, sampling rate and integration method may result in a product difference of ∼0.3, ∼0.15 and ∼0.05 millimeter geoid height, respectively. The total differences between HUST‐ERA5 and AOD1B‐RL06 can lead to a mean variation of ∼7.34 nm/s on the laser ranging interferometry (LRI) range‐rate residuals, for example, during January 2019, which is already close to the LRI precision. This impact is invisible for the GRACE (‐FO) gravity inversion because of the less accurate on‐board KBR (K‐band ranging) instrument, however, it will be non‐negligible and should be considered when the LRI completely replaces the KBR in the future gravity missions.
Cyclic Selection: Auxiliaries Are Merged, Not Inserted

Traditional approaches to verbal periphrasis (compound tenses) treat auxiliary verbs as lexical items that enter syntactic derivation like any other lexical item, via Selection/Merge. An alternative view is that auxiliary verbs are inserted into a previously built structure (e.g., Bach 1967, Arregi 2000, Embick 2000, Cowper 2010, Bjorkman 2011, Arregi and Klecha 2015). Arguments for the insertion approach include auxiliaries’ last-resort distribution and the fact that, in many languages, auxiliaries are not systematically associated with a given inflectional category (Bjorkman’s (2011) “overflow” distribution). Here, I argue against the insertion approach. I demonstrate that the overflow pattern and last-resort distribution follow from Cyclic Selection (Pietraszko 2017)—a Merge counterpart of Cyclic Agree (Béjar and Rezac 2009). I also show that the insertion approach makes wrong predictions about compound tenses in Swahili, a language with overflow periphrasis. Under my approach, an auxiliary verb is a verbal head externally merged as a specifier of a functional head, such as T. It then undergoes m-merger with that head, instantiating an External-Merge version of Matushansky’s (2006) conception of head movement.
Finding Hidden Outliers to Promote the Consistency of Key Morphological Traits and Phylogeny in Dennstaedtiaceae

With the development of open science and technological innovation, using sharing data and molecular biology techniques in the study of taxonomy and systematics have become a crucial component of plants, which undoubtedly helps us discover more hidden outliers or deal with difficult taxa. In this paper, we take Dennstaedtia smithii as an example, based on sharing molecular database, virtual herbarium and plant photo bank, to clarify the outliers that have been hidden in Dennstaedtia and find the key morphological traits with consistent of molecular systematics. In molecular phylogenetic analyses, we used rbcL, rps4, psbA-trnH and trnL-F sequences from 5 new and 49 shared data; the results showed that Dennstaedtia smithii is nested within Microlepia rather than Dennstaedtia. We further studied the morphological characters based on the phylogeny result and found that D. smithii is distinguished from other species of Dennstaedtia by spore ornamentation and the unconnected of grooves between rachis and pinna rachis. According to morphological and molecular phylogenetic studies, our results supported that D. smithii should be a new member of Microlepia and renamed Microlepia smithii (Hook.) Y.H. Yan. Finding hidden outliers can promote the consistency of morphological and molecular phylogenetic results, and make the systematic classification more natural.
Contract size changes in the options market: effects on market efficiency and investor behaviour

ABSTRACT We study options market participants’ trading behaviour before and after the options multiplier increases. After the options multiplier increases, the options market becomes more efficient. By analysing the high-frequency microstructure dataset, we show that local retail and local institutional investors who trade in both options and futures markets trade more after the change in the multiplier. Our results imply that the increase in the market efficiency may be caused by fewer speculators. In addition, lottery stocks are traded more actively after the options multiplier increase.
Rational-Critical User Discussions: How Argument Strength and the Conditions Set by News Organizations Are Linked to (Reasoned) Disagreement

Abstract Due to their potential influence on the individual and societal formation of opinions, the quality of online discussions has been a subject of widespread interest. From a deliberative perspective, rational argumentation and critical reflection are central criteria for good discourse. Drawing on research on the perception of arguments and the conditions of disagreement, we ask how argument strength is linked to the likelihood of receiving (reasoned) disagreement and whether the discussion norms and technical features set by the news organizations moderate this effect. Based on a manual content analysis of 14.690 user comments on nine German news websites, we find that comments with a higher argument strength are more likely to receive disagreement in general and reasoned disagreement in particular. Further, the levels of (reasoned) disagreement are higher on platforms with strong discussion norms and supporting technical features. The results show that the quality of a discussion can be related to both users’ argumentation and the decisions of the news organizations.
Earnings Management, Related Party Transactions and Corporate Performance: The Moderating Role of Internal Control

The primary purpose of this study is to investigate the impacts of earnings management (EM) and related party transactions (RPTs) on corporate financial performance in an emerging market, Iran. This paper also aims to examine the moderating role of internal control weakness (ICW) in the relationship between them. The study sample includes 108 Iranian manufacturing companies listed on the Tehran Stock Exchange (TSE) between 2013 and 2018, and panel data with random effects are used to test the hypotheses. When an accounting-based measure called ROA is defined as a proxy for corporate performance, the results show that there is a negative association between real earnings management (REM) and corporate financial situation, while accrual-based earnings management (AEM) and firm value are correlated positively. However, when Tobin’s Q index is defined as a proxy for corporate performance, we do not find any significant association between them. Consistent with the tunneling hypothesis or agency theory, our findings confirm RPTs damage corporate value (ROA and Tobin’s Q) because managers probably consider it a mechanism to exploit enterprise resources owing to existing conflictual interests. Moreover, purchase-related party transactions lead to lower ROA, whereas sale-related party transactions and Tobin’s Q are correlated negatively. Moreover, weak internal control has a positive moderating influence on the linkage between AEM and Tobin’s Q index. Finally, we provide robust evidence that there is a positive association between sale growth and institutional owners with ROA and Tobin’s Q, although financial leverage and mergers and acquisitions (M&A) have a destructive effect on corporate value.