Miracle-dz/UniFAR-FTUs huggingface dataset:
Natural infections of Pintomyia verrucarum and Pintomyia maranonensis by Leishmania (Viannia) peruviana in the Eastern Andes of northern Peru

The natural infection of sand flies by Leishmania was investigated in Andean areas located between the Central and Eastern Cordilleras of northern Peru where cutaneous leishmaniasis caused by Leishmania (Viannia) peruviana is endemic. Sand flies were captured at five locations along the Utcubamba River in the Department of Amazonas, and morphologically identified under a microscope. Among 422 female sand flies dissected, the most dominant species was Pintomyia verrucarum (320 flies), followed by Pi. maranonensis (83 flies), Pi. robusta (13 flies), and Lutzomyia castanea (6 flies). Genetic analysis of sand flies from these areas together with those from other areas revealed that individuals of Pi. verrucarum were closely related regardless of morphological variation of their spermathecae. On the other hand, individuals of Pi. maranonensis collected in the study area were distant from those of other areas with genetic distances over the intraspecific level but mostly below the interspecific level, suggesting the unique characteristics of sand flies in this area. The natural infection of sand flies by flagellate parasites was detected mainly in the hindgut of each one of Pi. verrucarum and Pi. maranonensis. Both parasite species were identified as L. (V.) peruviana based on cytochrome b and mannose phosphate isomerase gene analyses. In addition, parasite species obtained from the lesion of a patient with cutaneous leishmaniasis in the study area in this period was identified as L. (V.) peruviana. These results strongly suggest that Pi. verrucarum and Pi. maranonensis are responsible for the transmission of L. (V.) peruviana in these areas. This is the first report of the natural infection of Pi. maranonensis by L. (V.) peruviana.
Clinical Risk Profile for a Second Anterior Cruciate Ligament Injury in Female Soccer Players After Anterior Cruciate Ligament Reconstruction

Background: The risk of a second anterior cruciate ligament (ACL) injury when participating in pivoting sports after ACL reconstruction is high. Risk factors associated with a second ACL injury are complex. Purpose: To investigate the combinations of various clinical risk factors associated with second ACL injury in female soccer players with a primary unilateral ACL reconstruction, using Classification and Regression Tree (CART) analysis. Study Design: Cohort study; Level of evidence, 2. Methods: A total of 117 active female soccer players (mean ± SD age, 20 ± 2 years) were included. Athletes were enrolled 19 ± 9 months after ACL reconstruction and were prospectively followed for 2 years. At baseline, all players underwent assessment of knee and ankle joint range of motion (ROM), participated in functional tests (postural control, hop performance, and movement asymmetries in the lower limbs and trunk), and answered questionnaires (patient-reported knee function, knee-related quality of life, psychological and personality factors). A clinical prediction model using CART was developed. Results: A total of 28 players (24%) sustained a second ACL injury (21 ipsilateral and 7 contralateral ruptures) while playing soccer. CART analysis selected 9 of 19 independent variables associated with second ACL injury: the 5-jump test, knee collapse on the non–ACL reconstructed leg in a drop vertical jump, tuck jump, limb symmetry index on side hop and the single hop for distance, side difference in ankle dorsiflexion ROM, and scores for the questionnaires ACL-Return to Sport After Injury and the Swedish Universities Scales of Personality subscales of Stress Susceptibility and Adventure Seeking. The accuracy of the model was 89%, with 100% sensitivity and 76% specificity. CART analysis indicated that the interaction of longer jumps in the 5-jump test (>916 cm) with more side difference in ankle dorsiflexion ROM (>–2.5°) and more knee valgus collapse in the nonreconstructed knee (>−1.4 cm) (relative risk, 4.03; 95% CI, 2.21-7.36) best predicted an increased likelihood of a second ACL injury. Conclusion: The risk profiles selected by CART could accurately identify female soccer players at high risk for a second ACL injury. There was an interaction between functional performance, clinical assessment, and psychological factors, and it is reasonable to include these factors in return-to-sport decisions and in athlete screening after ACL injury.
Prevalence and predictors of work-related depression, anxiety, and stress among waiters: A cross-sectional study in upscale restaurants

Background Poor mental health often interrupts people’s regular activities making them unable to work effectively resulting in poor performance and high turnover intention. We examined the prevalence and predictors of depression, anxiety and stress among waiters in upscale restaurants. Methods This descriptive cross-sectional study involved 384 waiters in upscale restaurants in the Accra Metropolis. Data were collected using a pre-tested questionnaire which embedded DASS-21 (Cronbach Alpha = 0.815). The analysis included descriptive and inferential statistics using STATA 15. Statistical significance was set at p-value <0.05 at 95% confidence interval. Results The prevalence of depression was 38.3%, while anxiety and stress were 52.3% and 34.4% respectively. Females (AOR = 1.69, 95%CI = 1.02–2.79), waiters who foresee a better remuneration (AOR = 3.09, 95%CI = 1.95–4.87), consume caffeine (AOR = 1.44, 95%CI = 0.90–2.32), and use non-prescription drugs (AOR = 2.22, 95%CI = 1.39–3.55) were more likely to have depression. Females (AOR = 1.86, 95%CI = 1.17–2.96), those who foresee better remuneration (AOR = 2.85, 95%CI = 1.82–4.49), and those who use non-prescription drugs (AOR = 2.13, 95%CI = 1.38–3.28) were more likely to have anxiety. Females (AOR = 1.74, 95%CI = 1.01–2.99), waiters who are positive of career success (AOR = 1.70, 95%CI = 0.99–2.91), who foresee better remuneration (AOR = 2.99, 95%CI = 1.85–4.83), consume caffeine (AOR = 1.54, 95%CI = 0.93–2.54), and who use non-prescription drugs (AOR = 3.16, 95%CI = 1.93–5.17) were more likely to be stressed. Conclusion There is a high prevalence of poor mental health among waiters. Urgent intervention by hospitality stakeholders is needed to improve their working conditions and psychosocial health to accelerate progress towards the Sustainable Development Goal of promoting mental health and wellbeing.
Medical help-seeking intentions for cognitive impairment by the patient

Abstract Objectives: Older adults represent one of the fastest growing population groups. As the aged population increases, incidence of Alzheimer’s disease (AD) and other dementias will also increase. Professionals agree that early intervention is essential for therapeutic and quality of life purposes; however, many older adults wait several months or years to seek medical help after first noticing signs of cognitive impairment. The present study sought to identify the predictors of help-seeking for cognitive impairment by an individual for him/herself after the first detection of symptoms. Method: An online survey was administered to adults (N = 250) 50 years old and older. Individuals responded about their help-seeking intentions in response to a hypothetical vignette depicting symptoms of cognitive decline derived from a similar study with caregivers conducted by Qualls and colleagues. Additional standardized measures measuring constructs such as knowledge of Alzheimer’s disease were completed. Results: The present study reveals that cognitive (i.e. symptom identification and disease attribution) and affective (i.e. symptom impact and threat appraisal) factors, as well as an interaction between the two, are predictive of help-seeking intentions with excellent model fit. Conclusion: Help-seeking intentions by individuals with possible cognitive impairment are comparable to those of potential caregivers. Contrary to hypotheses, high threat appraisal positively predicted help-seeking intentions despite the expectation that threat-induced fear would lead to avoidance. Recommendations are made for future research to further investigate both patients’ help-seeking intentions and actions in response to signs of cognitive impairment. Supplemental data for this article is available online at https://doi.org/10.1080/13607863.2021. 1910791 .
“Be careful what you do”: How social threat influences social attention driven by reach-to-grasp movements

ABSTRACT Social attention is the ability to share with social partners the attentional focus on an object and to orient attentional resources after observing others’ behaviors. In the present work, we analyzed whether social threat may influence social attention during the observation of social agents’ reach-to-grasp movements. In two experimental studies, we adopted a Posner-like paradigm: participants were required to classify neutral stimuli appearing in a congruent or incongruent position with the actor’s movement. Membership (ingroup vs. outgroup) and contextual cues (neutral vs. threatening objects) were manipulated. The results showed a robust action-cueing effect: responses to stimuli in a congruent position with the actor’s action were faster than responses to incongruent stimuli. Interestingly, we found a stronger effect for the threatening outgroup (i.e., Iraqi) than for the ingroup (i.e., Italian), especially in presence of menacing objects. No effects were found when observing the non-threatening outgroup (i.e., Japanese).
Experimental in-vitro investigation on Epi-Off-Crosslinking on porcine corneas

Aim To evaluate quantitatively the effects of the Epi-Off-CXL irradiance dose on the stromal stiffening of pig corneas. Setting Laboratory of Biological structures (LaBS), Politecnico di Milano, Milano, Italy. Methods Inflation tests have been carried on 90 excised and de-epithelized pig corneas, monitoring the change of configuration of the corneal dome at specific pressures. Test have been carried out twice on each cornea, once before and once after Epi-Off-CXL performed at a constant irradiance of 9 mW/cm2 and variable UV-A exposure times. Corneas were grouped according to the exposure time (2.5, 5, 10, 15 and 20 min), proportional to the irradiation dose (1.35, 2.7, 5.4, 8.1, and 10.8 J/cm2). A theoretical model based on linearized shell theory has been used to estimate the increment of the corneal stiffness. Results The linearized shell theory allowed to establish a quantitative relation between the increment of the stiffness parameters and the irradiation dose. Relative to the pre-treatment values, in all experiments the post-treatment corneal stiffness revealed a pronounced increase. In general, the stiffness gain increased with the exposure time. No significant differences in stiffening was observed between tests conducted at 2.5, 5, and 10 min exposure. Conclusions Qualitatively, the effectiveness of accelerated CXL treatments observed in pig corneas complies very well with in-vivo clinical results in humans, suggesting that experimental data in pigs can be very useful for the design of the procedure in humans. A larger irradiation dose provides a larger increment of the corneal stiffness. Due to the biological variability of the tissues, however, it is difficult to distinguish quantitatively the level of the reinforcement induced by accelerated protocols (low doses with < = 10 min exposure), less prone to induce damage in the corneal tissue. Therefore, the definition of personalized treatments must be related to the actual biomechanics of the cornea.
New pectic polysaccharides from Codonopsis pilosula and C. tangshen: structural characterization and cellular antioxidant activities.

BACKGROUND Codonopsis pilosula and C. tangshen are plants widely used in traditional Chinese medicine. Two pectic polysaccharides from roots of C. pilosula and C. tangshen named as CPP-1 and CTP-1 were obtained by boiling water extraction and column chromatography. RESULTS The core structure of both CPP-1 and CTP-1 are long homogalacturonan regions (HG) region as the backbone, and the rhamnogalacturonan I (RG-I) region as the side chains. While CPP-1 has the methyl esterified galacturonic acid units and slightly lower molecular weight than CTP-1. The biological test suggested that CPP-1 and CTP-1 can protect IPEC-J2 cells against the H2 O2 -induced oxidative stress via up-regulating Nrf2 and related genes in IPEC-J2 cells. The different antioxidative activities of polysaccharides from different source of C. pilosula may be result of the difference in their structures. CONCLUSION All results indicated that pectic polysaccharides CPP-1 and CTP-1 from different species of C. pilosula roots could be used as a potential natural antioxidant source. These findings will be valuable for further studies and new applications of pectin-containing health products. This article is protected by copyright. All rights reserved.
A neurogenetic analysis of female autism

Abstract Females versus males are less frequently diagnosed with autism spectrum disorder (ASD), and while understanding sex differences is critical to delineating the systems biology of the condition, female ASD is understudied. We integrated functional MRI and genetic data in a sex-balanced sample of ASD and typically developing youth (8–17 years old) to characterize female-specific pathways of ASD risk. Our primary objectives were to: (i) characterize female ASD (n = 45) brain response to human motion, relative to matched typically developing female youth (n = 45); and (ii) evaluate whether genetic data could provide further insight into the potential relevance of these brain functional differences. For our first objective we found that ASD females showed markedly reduced response versus typically developing females, particularly in sensorimotor, striatal, and frontal regions. This difference between ASD and typically developing females does not resemble differences between ASD (n = 47) and typically developing males (n = 47), even though neural response did not significantly differ between female and male ASD. For our second objective, we found that ASD females (n = 61), versus males (n = 66), showed larger median size of rare copy number variants containing gene(s) expressed in early life (10 postconceptual weeks to 2 years) in regions implicated by the typically developing female > female functional MRI contrast. Post hoc analyses suggested this difference was primarily driven by copy number variants containing gene(s) expressed in striatum. This striatal finding was reproducible among n = 2075 probands (291 female) from an independent cohort. Together, our findings suggest that striatal impacts may contribute to pathways of risk in female ASD and advocate caution in drawing conclusions regarding female ASD based on male-predominant cohorts.
Analysis of avoidable cardiopulmonary resuscitation incidents with a part-time rapid response system in place

Background: Although a rapid response system (RRS) can reduce the incidence of cardiopulmonary resuscitation (CPR) in general wards, avoidable CPR cases still occur. This study aimed to investigate the incidence and causes of avoidable CPR. Methods: We retrospectively reviewed the medical records of all adult patients who received CPR between April 2013 and March 2016 (35 months) at a tertiary teaching hospital where a part-time RRS was introduced in October 2012. Four experts reviewed all of the CPR cases and determined whether each event was avoidable. Results: A total of 192 CPR cases were identified, and the incidence of CPR was 0.190 per 1,000 patient admissions. Of these, 56 (29.2%) were considered potentially avoidable, with the most common cause being doctor error (n=32, 57.1%), followed by delayed do-not-resuscitate (DNR) placement (n=12, 21.4%) and procedural complications (n=5, 8.9%). The percentage of avoidable CPR was significantly lower in the RRS operating time group than in the RRS non-operating time group (20.7% vs. 35.5%; P=0.026). Among 44 avoidable CPR events (excluding cases related to DNR issues), the rapid response team intervened in only three cases (6.8%), and most of the avoidable CPR cases (65.9%) occurred during the non-operating time. Conclusions: A significant number of avoidable CPR events occurred with a well-functioning, part-time RRS in place. However, RRS operation does appear to lower the occurrence of avoidable CPR. Thus, it is necessary to extend RRS operation time and modify RRS activation criteria. Moreover, policy and cultural changes are needed prior to implementing a full-time RRS.
Measures of resting state EEG rhythms for clinical trials in Alzheimer's disease: Recommendations of an expert panel

The Electrophysiology Professional Interest Area (EPIA) and Global Brain Consortium endorsed recommendations on candidate electroencephalography (EEG) measures for Alzheimer's disease (AD) clinical trials. The Panel reviewed the field literature. As most consistent findings, AD patients with mild cognitive impairment and dementia showed abnormalities in peak frequency, power, and “interrelatedness” at posterior alpha (8‐12 Hz) and widespread delta (< 4 Hz) and theta (4‐8 Hz) rhythms in relation to disease progression and interventions. The following consensus statements were subscribed: (1) Standardization of instructions to patients, resting state EEG (rsEEG) recording methods, and selection of artifact‐free rsEEG periods are needed; (2) power density and “interrelatedness” rsEEG measures (e.g., directed transfer function, phase lag index, linear lagged connectivity, etc.) at delta, theta, and alpha frequency bands may be use for stratification of AD patients and monitoring of disease progression and intervention; and (3) international multisectoral initiatives are mandatory for regulatory purposes.
Risk-Taking Behavior in Recovered COVID-19 Patients.

BACKGROUND The aim of this study is to investigate risk-taking behavior and decision-making processes in recovered COVID-19 patients. SUBJECTS AND METHODS Twenty patients recovered from COVID-19 as confirmed by polymerase chain reaction (PCR) tests and twenty-one healthy individuals were recruited. A computerized version of the Iowa Gambling Test (IGT) for measuring risk-taking behavior tendencies as a decision-making process and State-Trait Anxiety Inventory (STAI), Beck Depression Inventory (BDI), and WMS-R Digit Span Forward Test (DSFT) for clinical assessments included. The assessments of the recovered patients were applied on the initial phase that the tests of the patients were negative and on the 4-week follow up phase. RESULTS The results showed that the anxiety scores were significantly higher in the healthy control group than in the group of recovered patients. The IGT-Net 4 scores were significantly and IGT-Net total scores were marginally significantly lower in the group of recovered patients. In other words, recovered patients showed higher risk-taking behavior tendencies. This tendency difference is consistent with the anxiety levels of the groups. These IGT scores showed to be persistent in the 4-week follow up phase. CONCLUSIONS Our findings indicate that recovered patients show higher risk-taking behavior tendencies than healthy controls and this may be the result of overcoming the COVID-19 threat.
Single phase, high hole mobility Cu2O films as an efficient and robust hole transporting layer for organic solar cells

Inorganic hole transport materials (HTMs) are being extensively studied as they are promising for efficient and stable organic solar cells (OSCs) and organic–inorganic hybrid perovskite solar cells. High mobility, earth-abundant, environmentally stable and nontoxic HTMs are the focus of research. This work demonstrates that highly transparent, high mobility and phase pure Cu2O nano-crystal films are promising HTMs for efficient OSC applications. The Cu2O films are synthesized by reactive magnetron sputtering at room temperature. The highest power conversion efficiency of OSCs based on the classical PTB7:PC71BM active layer reaches 8.61% with the Cu2O HTM, which is 15% higher than that of the OSCs with the standard PEDOT:PSS HTM layer. Our study shows that the device based on the Cu2O HTM exhibited better energy level alignment, reduced series resistance and therefore improved charge extraction compared with those based on CuO and PEDOT:PSS HTM layers. The improved photovoltaic performance suggests that Cu2O is a promising HTM for future low temperature roll to roll organic solar cell and even perovskite solar cell fabrication.
Breaching the phagosome, the case of the tuberculosis agent

The interactions between microbes and their hosts are among the most complex biological phenomena known today. The interaction may reach from overall beneficial interaction, as observed for most microbiome/microbiota related interactions to interaction with virulent pathogens, against which host cells have evolved sophisticated defence strategies. Among the latter, the confinement of invading pathogens in a phagosome plays a key role, which often results in the destruction of the invader, whereas some pathogens may counteract phagosomal arrest and survive by gaining access to the cytosol of the host cell. In the current review, we will discuss recent insights into this dynamic process of host‐pathogen interaction, using Mycobacterium tuberculosis and related pathogenic mycobacteria as main examples.
Salience of spatiochromatic patterns

We investigated the dependence of perceived contrast on cone-opponent stimulus content and its spatial distribution. Participants matched a comparison patch to a light gray standard of fixed contrast. The first experiment determined the point of iso-salience for gratings, Gabors and Gaussians along cardinal directions in cone-opponent color space for two-alternative forced choice (2AFC) and adjustment tasks. No difference was found between adjustment and 2AFC tasks, meaning that adjustment tasks provide a quick and robust way to measure perceived contrast, at least for relatively large suprathreshold stimuli. In line with the differences in contrast energy between Gaussians, Gabors, and gratings, Gaussians required less contrast to achieve equal perceived salience with a standard irrespective of color. More surprisingly, bluish Gaussians were found to have higher salience than yellowish Gaussians at equal levels of contrast. Although perceived contrast of grating and Gabor patterns likely depends on spatial frequency channels that at 1 cycle-per-degree are not too dissimilarly tuned for color and luminance, for Gaussians the contribution of single-opponent neurons would be greater for color than for luminance. In a follow-up experiment, we found that the bluish/yellowish asymmetry decreased as we reduced the proportion of the lowpass non-flat contrast distribution in the stimulus, with minimal asymmetry for the stimulus with a flat contrast distribution (i.e., uniform patch). Combined, this means that differential engagement of spatial frequency channels, single-opponent and double-opponent neurons impacts on perceived contrast of chromatic suprathreshold stimuli. Perceived contrast thus provides a window into neural computations enacted by low-level cone-opponent mechanisms.