Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction of an ultrahigh-density linkage map and graphical representation of the arrangement of transcriptome-based unigene markers on the chromosomes of Allium cepa L.

BackgroundGenomic information for Allium cepa L. is limited as it is heterozygous and its genome is very large. To elucidate potential SNP markers obtained by NGS, we used a complete set of A. fistulosum L. -A. cepa monosomic addition lines (MALs) and doubled haploids (DHs). These were the parental lines of an A. cepa mapping population for transcriptome-based SNP genotyping.ResultsWe mapped the transcriptome sequence reads from a series of A. fistulosum-A. cepa MALs onto the unigene sequence of the doubled haploid shallot A. cepa Aggregatum group (DHA) and compared the MAL genotype call for parental bunching onion and shallot transcriptome mapping data. We identified SNP sites with at least four reads on 25,462 unigenes. They were anchored on eight A. cepa chromosomes. A single SNP site was identified on 3,278 unigenes and multiple SNPs were identified on 22,184 unigenes. The chromosome marker information was made public via the web database Allium TDB (http://alliumtdb.kazusa.or.jp/). To map these markers, we gathered RNA sequence data from 96 lines of a DHA × doubled haploid bulb onion A. cepa common onion group (DHC) mapping population. After selecting co-dominant SNP sites, 16,872 SNPs were identified in 5,339 unigenes. Of these, at least two SNPs with identical genotypes were found in 1,435 unigenes. We developed a linkage map using genotype information from these unigenes. All unigene markers mapped onto the eight chromosomes and graphical genotyping was conducted based on the unigene order information. Another 2,963 unigenes were allocated onto the eight chromosomes. To confirm the accuracy of this transcriptome-based genetic linkage map, conventional PCR-based markers were used for linkage analysis. All SNP - and PCR-based markers were mapped onto the expected linkage groups and no inconsistency was found among these chromosomal locations.ConclusionsEffective transcriptome analysis with unique Allium resources successfully associated numerous chromosome markers with unigene information and an ultrahigh-density A. cepa linkage map. The information on these unigene markers is valuable in genome sequencing and useful trait detection in Allium.
Change in Content and Chemical Composition of Hypericum perforatum L. Oil at Three Harvest Time

ABSTRACT St. John's wort is an important medicinal plant that contained a wide range of secondary metabolites such as naphthodianthrones, phloroglucinols and essential oil. The quality of Hypericum perforatum was determined by several cultural practices. In this research essential oil content and composition determined at before flowering, full flowering and fruit set stages. Water distillation was used for essential oil extraction. Essential oil composition was determined by GC and GC/MS. On the basis of the results herb of Saint John's wort in full flowering stage has higher amount of essential oil (0.35 ml/100 g dry weight) than before flowering and fruit set stage (0.12 and 0.16 ml/100 g dry weight, respectively). The essential oil contains longifolene, α- and γ-eudesmol, spathulenol, bicyclogermacrene, β-caryophyllene, α-cadinol, α-cadinene and β-bisabolene as major constituents. These constituents are affected by harvest time. In this respect longifolene is identified in oil sample before flowering and at fruit set stage (18.71 and 21.99%, respectively) but unidentified in the oil of full flowering stage. The amount of bicyclogermacrene was the highest (16.93%) at full flowering stage and decreased sharply at other harvest time. According to the results the most suitable time for harvesting of Hypericum perforatum with respect to essential oil content and composition is full flowering.
Hygrometric Half-lives of Museum Cases

The moisture-buffering capability of four types of museum cases, Solander boxes, portfolio boxes, archival document cases and music boxes, was evaluated. An expression for hygrometric half-life was derived and the half-lives were compared with high, as well as low ambient relative humidity for the four types of cases in terms of their materials of construction and closure characteristics
The study of reading fluency and reading comprehension skills of primary school students whose mother tongue is different

The purpose of this research is to analyse reading fluency and comprehension skills of different mother tongue of primary school students in the 4th grade. The effects of variables such as mother tongue and gender were examined in reading fluency and comprehension skills of different primary school students whose mother tongue is different. The working group of the study was selected by criterion sampling from the purposeful sampling methods among the students studying in primary schools in the province of Tuşba in Van. The working group consists of 201 female and 227 males, a total of 428 students. In the research, ‘Narrative Text’ collected data about reading fluency; ‘Multidimensional Fluency Scale’ measured reading prosody skills; and ‘Reading Comprehension Test’ was used to determine the reading comprehension levels. According to the findings, the students were generally divided into two groups: those whose mother tongue is Turkish, and those who is Kurdish. As a result of the research, it was revealed that the fluent reading and reading comprehension skills of primary school 4th grade students whose mother tongue is Turkish are generally higher than those whose mother tongue is Kurdish.
Going back and researching in the Pacific community

The move to focus on Pacific indigenous research methodologies and methods is a complex yet ongoing experience for researchers of Pacific heritage. The relational positionalities of Pacific researchers allow a move away from a dual or binary perspective of one’s research responsibilities to a more fluid understanding of what it means to do research by, for, with Pacific communities. This paper highlights the diverse experiences of three Pacific researchers taking into consideration heritage connections, socio-cultural backgrounds and research contexts. We utilise talanoa as a method of engagement, reflexivity, and sharing of our experiences with Tongan, Samoan and Fijian communities. We argue that talanoa as a Pacific research method enables the diverse layers of experiences that take into particular consideration our connections to land, people and knowledges in the diaspora.
Investigation of the effect of Delayed Auditory Feedback and transcranial Direct Current Stimulation (DAF-tDCS) treatment for the enhancement of speech fluency in adults who stutter: a Randomized Controlled Trial

Background: With a population prevalence of one percent, stuttering is among the main speech pathology-related topics of research. Adults who stutter may benefit from transcranial direct current stimulation (tDCS) as an adjunctive intervention for enhancing speech fluency. In this study, Delayed Auditory Feedback (DAF) was combined with tDCS applied over the superior temporal gyrus. It was anticipated that the combined intervention cause improvements in speech fluency.Methods: A randomized, double-blind, sham-controlled clinical trial was conducted to investigate the effectiveness of intervention in enhancing speech fluency. Fifty participants were randomly allocated to the intervention or control group. In the intervention group, participants received DAF combined with anodal tDCS, while the control group was exposed to sham tDCS simultaneously with DAF. Each subject participated in six intervention sessions. Speech fluency was assessed as the baseline, before intervention as well as immediately, one week and six weeks after intervention. Results: In the intervention group, the percentage of stuttered syllables was significantly reduced immediately, one week and six weeks after the intervention, as compared with the control group. The scores of the Stuttering Severity Instrument, also showed a significant reduction in the intervention group compared with the control group. No significant difference was found in the Overall Assessment of the Speaker’s Experience of Stuttering questionnaire scores between the two groups after intervention. Conclusion: The results of this study propose anodal tDCS as an adjunctive method to increase speech fluency in stuttering for a prolonged time course after intervention, when combined with fluency therapy.Trial registration: This trial was registered in ClinicalTrial.gov before recruiting the subjects. The registration number is NCT03990168 and the date of registration is June 18, 2019. https://clinicaltrials.gov/ct2/show/NCT03990168
RADIOGRAPHIC AND IMMUNO HISTOCHEMICAL DIAGNOSTIC STUDY OF STRONTIUM RANELATE AND METAL-SUBSTITUTED HYDROXYAPATITE BONE GRAFT MATERIALS IN DIABETES MELLITUS WITH CHRONIC PERIODONTITIS

Objective: The aim of the present study is to assess the radiographic and immunohistochemical effect of strontium ranelate (SR) and metal substituted hydroxyapatite (MSHA) on the treatment of chronic periodontitis among diabetic rats Materials and Methods: The study involved ten adult male and female rice rats (1-month-old) weighting (250- 300g). After a 24-hour fast, a single intraperitoneal dose of freshly prepared alloxan was injected to induce diabetes. A month after the injection of alloxan, the rats were randomly assigned to one of the two treatment modalities: SR with gengigel or MSHA with gengigel. Digital periapical radiography was taken at baseline, a month after the injection of alloxan to see resorbed alveolar bone and after 3months post-operative surgery for radiographic assessment. The diabetic rats were sacrificed using an overdose of anesthesia, and gingival tissue samples were collected. The specimens were processed for hematoxylin and eosin (H & E) staining and immune stain for expression of matrix metalloproteinases 2 (MMP-2). Results: Digital periapical radiography showed an increase of nearly 0.37 mm in the height of the alveolar bone in the side of the SR group while the other side in the MSHA group increased by nearly 0.12 mm. A statistically significant reduction in the expression of MMP2 in the SR group as compared to the MSHA group was found upon comparing the immunohistochemical results of the 2 groups. Conclusion: Radiographic and immunohistochemical results showed that SR was a promising material in the treatment of periodontal diseases.
Which is Worse; Divorce or Conflict? Parental Divorce, Interparental Conflict, and its Impact on Romantic Relationship Quality of Young Dating Adults in the Maldives

Past research has documented the negative impact of parental divorce and interparental conflict on romantic relationship quality but in isolated studies. Limited studies have compared both parental divorce and interparental conflict on young adult’s dating relationships. This study aimed to fill this gap by identifying the roles of parental divorce and interparental conflict and how they interact on the relationship between parental divorce, interparental conflict, and romantic relationship quality of young adults. A total of 297 Maldivian undergraduate students, who were in a dating romantic relationship, between the ages of 18 to 25 participated in the study. Data was collected using a self-administered set of questionnaires. Relationship quality was measured using the Relationship Quality Component Inventory and perceived interparental conflict was measured using Children’s Perception of Interparental Conflict Scale (CPIC). The collected data were analyzed using IBM Statistical Package for Social Sciences (SPSS) and PLS-SEM software. Results revealed that parental divorce and interparental conflict was negatively associated with low romantic relationship quality. Additionally, Perceived interparental conflict mediated the relationship between parental divorce and relationship quality of young dating adults.
Sequential exhaustive extraction of a Mollisol soil, and characterizations of humic components, including humin, by solid and solution state NMR

A comprehensive sequential extraction procedure was applied to isolate soil organic components using aqueous solvents at different pH values, base plus urea (base‐urea), and finally dimethylsulfoxide (DMSO) plus concentrated H2SO4 (DMSO‐acid) for the humin‐enriched clay separates. The extracts from base‐urea and DMSO‐acid would be regarded as ‘humin’ in the classical definitions. The fractions isolated from aqueous base, base‐urea and DMSO‐acid were characterized by solid and solution state NMR spectroscopy. The base‐urea solvent system isolated ca. 10% (by mass) additional humic substances. The combined base‐urea and DMSO‐acid solvents isolated ca. 93% of total organic carbon from the humin‐enriched fine clay fraction (<2 μm). Characterization of the humic fractions by solid‐state NMR spectroscopy showed that oxidized char materials were concentrated in humic acids isolated at pH 7, and in the base‐urea extract. Lignin‐derived materials were in considerable abundance in the humic acids isolated at pH 12.6. Only very small amounts of char‐derived structures were contained in the fulvic acids and fulvic acids‐like material isolated from the base‐urea solvent. After extraction with base‐urea, the 0.5 m NaOH extract from the humin‐enriched clay was predominantly composed of aliphatic hydrocarbon groups, and with lesser amounts of aromatic carbon (probably including some char material), and carbohydrates and peptides. From the combination of solid and solution‐state NMR spectroscopy, it is clear that the major components of humin materials, from the DMSO‐acid solvent, after the exhaustive extraction sequence, were composed of microbial and plant derived components, mainly long‐chain aliphatic species (including fatty acids/ester, waxes, lipids and cuticular material), carbohydrate, peptides/proteins, lignin derivatives, lipoprotein and peptidoglycan (major structural components in bacteria cell walls). Black carbon or char materials were enriched in humic acids isolated at pH 7 and humic acids‐like material isolated in the base‐urea medium, indicating that urea can liberate char‐derived material hydrogen bonded or trapped within the humin matrix.
Case Report: Covid-19 And Severe Malaria Co-Infection

Background:A mysterious pneumonia case was first reported in Wuhan, China in December 2019 The World Health Organization (WHO) later named this pneumonia Coronavirus Disease (Covid-19) caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus The massive spread of Covid-19 increases the risk of co-infection with other pre-existing pathogens including malaria Case Report: A 32 years old woman was admitted to the hospital due to complaints of jaundice, fever, dyspneu and andominal pain On further examination of the malaria smear, plasmodium falciparum was found in the form of trophozoites and gametocytes, the parasite count was 119,902 U/L and a nasopharyngeal PCR swab was positive for SARS CoV-2 The patient conditionimprove after administering artesunate and oseltamivir Discussion: Several clinical manifestations are overlapping between Covid-19 and malaria, especially extrapulmonary manifestations of Covid-19 infection, including thrombocytopenia, elevated transaminase enzymes, increased bilirubin, and impaired kidney function Management was carried out to deal with malaria and Covid-19 simultaneously and continuously
Persistence of interference from L1 Arabic in written Hebrew

As the official and predominant public language in Israel, Hebrew is taught in Arab minority schools, mostly by L1 Arabic-speaking teachers. Active acquisition of Hebrew accelerates in the immersion condi- tions of high education. I explore the persistence of very common interference errors in various linguistic domains, as established by teachers’ written corrective feedback, and the correlation between persis- tence, error salience and a general learner effect. From a corpus of 56 Hebrew essays written by 9th graders, 11th graders and undergraduate students in southern Israel, the 14 most frequent interference errors were isolated and incorporated in a compiled test essay, which was then given to 13 L1 Arabic-speaking teachers of Hebrew to correct. The salience of each item was established by the percentage of teachers correcting it; each was also gra ded for its status as a general learners’ error. Statistical analysis showed a significant correlation between each of these two measures and persistence over the time pe- riod studied. This corroborates a multiple effect approach to persistence. Localized errors of phonology, orthography, and morphology generally declined faster than syntactic errors, which persisted especially in structures that occur in L1 Hebrew, marked for discourse-pragmatic effects.
Study of the Adsorption of Ozone on the Surface of Ferric Oxide by Reversed-Flow Inverse Gas Chromatography

Abstract In this work, in order to describe the impact of ozone on ferric oxide – a common constituent of building materials – in purely scientific terms, a novel methodology that of Reversed Flow–Inverse Gas Chromatography (RF–IGC or RF–GC) is proposed. Five important physicochemical quantities concerning adsorption of ozone on the solid surface of ferric oxide are determined, in five temperatures, in a time-resolved way. By means of a simple PC-program, the values of local adsorption energy, local adsorption isotherm, local monolayer capacity, probability density function for adsorption energy, and energy from lateral interactions are calculated. Thus, the role of surface heterogeneity, lateral interactions and different distributions of active sites (surface topography) on the adsorption of ozone on the surface of ferric oxide is analyzed in the way the RF–IGC method has it developed.
Is Ultra-Broadband Enough? The Relationship between High-Speed Internet and Entrepreneurship in Brandenburg

Recent improvements in broadband infrastructure in Europe have introduced augmented possibilities for both businesses and individuals. As expected, the provision of high-speed internet connections for European users will be an important step towards Industry 4.0 as well as a better entrepreneurial milieu. Still, though scientific literature is replete with examples of how modern communication technologies boost productivity and economic performance, not much research indicates how broadband technologies may bolster entrepreneurship, especially at a very detailed, municipal level. The aim of this study was to find out whether high-speed broadband provision had any effect on higher firm entry rates in Brandenburg, Germany. This region was chosen because of its favorable position in the vicinity of the capital state, Berlin. We carried out an ordinary less square estimated regression analysis using a dataset built for 417 municipalities. The results show that ultra-broadband provision had no significant impact on well-performing municipalities and those located close to Berlin, with no impact in the general model, and also had a negative correlation in underperforming and peripheral areas (significant at a 5% level). This leads to the conclusion that advanced broadband infrastructure does not automatically translate into higher firm entry rates; there is no such urgency in the need for speed , as it might appear at first glance.
Phase transition of lattice polymer systems

Monte Carlo simulations of a lattice polymer system of chains of N=20 segments each, comprising a total of 21 chains on a 21×21 square lattice, are carried out in order to clarify recent controversies concerning statistical thermodynamics of lattice models of polymers. Assuming an energy e≳0 for every right‐angle step, we find a first‐order phase transition of disordered phase to an ordered state at kBTc/e≊0.93, as demonstrated by discontinuities in the internal energy (conformational order) and the orientational order parameter. Comparison of our results with the predictions of the mean‐field lattice theory of Flory and the implications of these findings on phase transitions of real polymer systems are discussed.
Evaluation of Free Radical Scavenging Activities Against Multiple Heavy Metals Stress in Avicennia marina (Forsk.) and Rhizophora mucronata Lamk

The tolerance mechanism of mangrove species (Avicennia marina (Forsk.) and Rhizophora mucronataLamk) was studied by inducing multiple heavy metal stress in roots and leaves of the plants. Mangrove seedlings weretreated with different concentrations of heavy metal solution (Cr, Cd, Pb and Hg) for a period of 2 months. Scavengingantioxidant enzymes like Polyphenol Oxidase (PPO), Glutathione-S-transferase (GSTs) and Guaiacol Peroxidase(POD) were analyzed in both species after appropriate intervals of 15 days. Results revealed that activities of theseantioxidative enzymes were altered due to multiple heavy metals exposure in both mangrove species, whereas leavesexhibited the higher values as compared to the roots. In the leaves of both mangrove plants GSTs exhibited anincreased trend throughout the investigated period whereas, PPO activity initially showed an increasing trend, butgradually decreased with the passage of time in response to heavy metal stress. Furthermore, an increased level ofantioxidants was also observed in A. marina than R. mucronata which proves that the former is a strong candidate forheavy metals phyto-remediation with its viable survival strategies.
Counterproductive Work Behaviour: The Role of Work Overload and Organizational Justice Dimensions among Employees in Nigeria Private Sector Organizations

Due to the growing employment rate, employees’ voices may not be heard but they speak in salient ways especially regarding their organizational climate as regards work load and uneven distribution of organizational rewards and punishments. Considering that these circumstances elicit employees’ reaction, this study explored the roles of work overload and organizational justice dimensions on counterproductive work behaviour (CWB). The design was correlation design while regression statistics was used to analyze the predictive effects of work overload and organizational justice dimensions on CWB. Sample for the study was 85 employees, 44 males (51.8%) and 41 females (48.2%) drawn with the aid of purposive and cluster sampling. Participants’ ages range from 20 to 57 at an average age of 34.02 years. CWB scale, Distributive justice index, and Work overload scale were instruments for data collection. The result revealed that employees were high on counterproductive work behaviour and that the predictive effects of work overload on CWB was positive and significant at β(3, 385) = . 57*, p < . 05 implying that with greater work overload on employees CWB increased proportionally. However, predictive effects of distributive justice (DJ) and procedural justice (PJ) were negative and significant at β(3, 385) = . 52 and . 40, p < . 05 respectively implying that as the justice dimensions (distributive and procedural) improved, CWB reduced proportionally. The study recommends that management of organizations should shun exploitation and enthrone fair treatment of employees across board irrespective of ethnic background, age, religion, and gender to reduce CWB and improve organizational efficiency.