Miracle-dz/UniFAR-FTUs huggingface dataset:
Tympanal ears mediate male–male competition, courtship and mating success in Bicyclus anynana butterflies

The presence of intra-specific acoustic communication in diurnal butterflies is not well established. Here, we examined the function of the tympanal ear (Vogel’s organ, VO) in the seasonally polyphenic butterfly Bicyclus anynana in the context of sexual signalling. We investigated how the VO and the flanking enlarged veins, which are suggested sound resonance chambers, scale with wing size across sexes and seasonal forms, and how disruptions to the VO alter courtship behaviour and mating outcomes. We found that males have VOs similar in size to females despite having smaller wings, and dry season (DS) male cubital and anal veins do not scale with the wing size. This suggests that the VO plays an important role in males and that cubital and anal veins in DS males may be tuned to amplify specific sound frequencies. Behavioural assays performed with deafened and hearing males of different seasonal forms, in pair and triad settings, showed that deafened DS males, but not wet season males, experienced lower mating success relative to their hearing counterparts. Our study documents a novel function for the wing tympanal membrane in mediating courtship and mating outcomes in diurnal butterflies.
Phenobarbital suppresses growth and accelerates restoration of differentiation markers of primary culture rat hepatocytes in the chemically defined hepatocyte growth medium containing hepatocyte growth factor and epidermal growth factor.

Phenobarbital (PB), a liver-tumor promoter, at a concentration of 3 mM dramatically inhibited the growth of adult rat hepatocytes in the chemically defined medium, HGM, with added hepatocyte growth factor (HGF) and epidermal growth factor (EGF). In concurrence with these findings, PB down-regulated expression of the HGF receptor (c-met) and suppressed production of the autocrine growth factor transforming growth factor-alpha (TGF-alpha). Furthermore, PB down-regulated expression of transcription factors associated with proliferation such as AP1 and NF-kappaB. In the presence of PB, hepatocytes remained morphologically differentiated and restoration of the expression of mature hepatocyte markers, such as albumin and cytochrome P450s (1A, 2B1/2, and 2E1), was accelerated after an initial phase of growth. Additionally, PB strongly suppressed expression of the mRNA for alpha-fetoprotein, a protein primarily expressed by fetal liver, and the accelerative effect of PB on restoration of mature hepatocyte markers showed a correlation with the up-regulation of the hepatocyte-enriched transcription factors HNF3 and HNF4. When the effects of PB on various extracellular matrix proteins were examined, the data indicated that PB specifically suppressed laminin and fibronectin production by hepatocytes, suggesting an important role for these proteins in growing hepatocyte cultures.
TarO-specific inhibitors of wall teichoic acid biosynthesis restore β-lactam efficacy against methicillin-resistant staphylococci

New inhibitors of wall teichoic acid biosynthesis restore susceptibility of drug-resistant staphylococci to β-lactam antibiotics. Addressing antibiotic resistance with nonantibiotic adjuvants Coupled with the crisis in antibiotic drug resistance is a dearth of mechanistically new classes of antibacterial agents. One possible solution to this problem is to improve the efficacy of existing antibiotics against otherwise resistant bacteria using a combination agent approach. Lee et al. now describe just such a combination agent strategy to resuscitate the efficacy of β-lactam antibiotics. They identify nonantibiotic adjuvants termed tarocins that restore the killing activity of β-lactams against methicillin-resistant staphylococci, thereby enabling the application of β-lactams to treat Gram-positive bacterial infections. The widespread emergence of methicillin-resistant Staphylococcus aureus (MRSA) has dramatically eroded the efficacy of current β-lactam antibiotics and created an urgent need for new treatment options. We report an S. aureus phenotypic screening strategy involving chemical suppression of the growth inhibitory consequences of depleting late-stage wall teichoic acid biosynthesis. This enabled us to identify early-stage pathway-specific inhibitors of wall teichoic acid biosynthesis predicted to be chemically synergistic with β-lactams. We demonstrated by genetic and biochemical means that each of the new chemical series discovered, herein named tarocin A and tarocin B, inhibited the first step in wall teichoic acid biosynthesis (TarO). Tarocins do not have intrinsic bioactivity but rather demonstrated potent bactericidal synergy in combination with broad-spectrum β-lactam antibiotics against diverse clinical isolates of methicillin-resistant staphylococci as well as robust efficacy in a murine infection model of MRSA. Tarocins and other inhibitors of wall teichoic acid biosynthesis may provide a rational strategy to develop Gram-positive bactericidal β-lactam combination agents active against methicillin-resistant staphylococci.
THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications

Global cannabis use has risen 23% since 2010, with 209 million reported users, most of whom are males of reproductive age. Delta-9-tetrahydrocannabinol (THC), the main psychoactive phytocannabinoid in cannabis, disrupts pro-homeostatic functions of the endocannabinoid system (ECS) within the male reproductive system. The ECS is highly involved in regulating morpho-functional and intrinsic sperm features that are required for fertilization and pre-implantation embryo development. Previous work by our group demonstrated that THC altered sperm capacitation and the transcriptome, including several fertility-associated microRNAs (miRs). Despite the prevalent use of cannabis among males of reproductive age, clinical and pre-clinical research investigating the impact of paternal cannabis on sperm function and the outcomes of artificial reproductive technologies (ARTs) remains inconclusive. Therefore, the present study investigates the impact of in vitro THC exposure on morpho-functional and intrinsic sperm functions, including contributions to embryo development following IVF. Bovine sperm were used as a translational model for human and treated with concentrations of THC that reflect plasma levels after therapeutic (0.032μM), and low (0.32μM)-high (4.8μM) recreational cannabis use. After 6-hours of treatment, THC did not alter the acrosomal reaction, but 4. 8μM significantly reduced mitochondrial membrane potential (MMP) (p<0.05), primarily through agonistic interactions with CB-receptors. Fertilization of bovine oocytes with THC-treated sperm did not alter developmental rates, but blastocysts generated from sperm treated with 0.32–4. 8μM THC had fewer trophoblasts (p<0.05), while blastocysts generated from sperm exposed to any concentration of THC had fewer cells in the inner cell mass (ICM), particularly within the 0. 032μM group (p<0.001). Fertility associated miRs, including miR-346, miR-324, miR-33b, and miR-34c were analyzed in THC-exposed sperm and associated blastocysts generated by IVF, with lower levels of miRs-346, -324, and -33b found in sperm treated with 0.32μM THC, while miR-34c levels were higher in sperm treated with 0. 032μM THC (p<0.05). Levels of miR-346 were also lower in sperm treated with 0. 032μM THC, but higher in blastocysts generated from sperm exposed to 0.32μM THC (p<0.05). Our findings suggest that THC may alter key morpho-functional and epigenetic sperm factors involved in fertilization and embryo development. This is the first study to demonstrate that sperm exposed to THC in vitro negatively affects embryo quality following IVF.
Estimation of Adult Sex Ratio and Size-Related Sexual Dimorphism Based on Molecular Sex Determination in the Vulnerable La Selle Thrush, Turdus swalesi

Simple Summary This study assessed the adult sex ratio and sexual dimorphism in body size in the vulnerable La Selle Thrush, Turdus swalesi, over a 26-month period, in Haiti. We found that the overall adult sex ratio was significantly male-biased. However, this was only true during the non-breeding season, suggesting that males may show more site tenacity than females, although true differences in adult survival between males and females cannot be ruled out. We observed a slight, albeit significant, sexual dimorphism in size, with males having, on average, both a longer wing chord and a longer tail than females. Tail length was the best predictor of sex in a logistic regression model, with about 80% of individuals being correctly assigned to their actual sex. Understanding the causes and consequences of the observed male-biased sex ratio might be important for the conservation of the La Selle Thrush, particularly in the face of ongoing severe deforestation in Haiti. Abstract Sex-determination is of particular importance in avian ecology and conservation. However, many bird species show no conspicuous sexual dimorphism, such as the La Selle Thrush, Turdus swalesi, a vulnerable species endemic to Hispaniola. We captured individuals in southeastern Haiti, in 2019–2022. For each one, we collected contour feathers or blood samples for molecular sex identification based on the CHD-1 gene. In addition, we took body measurements of several captured individuals and recorded their weight. Out of a total of 65 birds, 45 were identified as males and 20 as females, indicative of a significantly male-biased sex ratio. However, analyses of first captures showed that the sex ratio at our study site was male-biased only outside of the breeding season, suggesting that females may disperse at that time while males remain on their territories. Sexual dimorphism was limited to wing chord length and tail length, with males being larger than females. Tail length was the best predictor of sex in a logistic regression model and correctly classified about 80% of individuals as male or female. We discuss our results in relation to previous studies of sex ratio and sexual dimorphism in turdid species and address their relevance for the conservation of avian species in one of the major protected forest areas in Haiti.
Optimal floodlight illumination of stages

1. Abstract In this video we present an algorithm finding an optimal illumination of a segment by a collection of floodlights. The input of the algorithm are the points at which the floodlights must originate, as well as the position of the segment. The illumination is said to be optimal if the sum of the floodlight angles is the smallest possible. The algorithm works in optimal O(rt log n) time and O(n) space.
Conceptualizing Willingness to Communicate in a L2: A Situational Model of L2 Confidence and Affiliation

Why do some students seek, while others avoid, second language (L2) communication? Many language teachers have encountered students high in linguistic competence who are unwilling to use their L2 for communication whereas other students, with only minimal linguistic knowledge, seem to communicate in the L2 whenever possible. Despite excellent communicative competence, spontaneous and sustained use of the L2 is not ensured. A colleague, who teaches a L2 and whose L2 competence is excellent, is well known to avoid “like the plague” L2 communication in social settings. A related observation is that many learners have noticed that their willingness to communicate (WTC) varies considerably over time and across situations. Our aim in this article is twofold. First we wish to provide an account of the linguistic, communicative, and social psychological variables that might affect one's “willingness to communicate. ” As demonstrated in the text below, and examination of WTC offers the opportunity to integrate psychological, linguistic, and communicative approaches to L2 research that typically have been independent of each other. We view the WTC model as having the potential to provide a useful interface between these disparate lines of inquiry. Our second goal is to suggest potential relations among these variables by outlining a comprehensive conceptual model that may be useful in describing, explaining, and predicting L2 communication. In an effort to move beyond linguistic or communicative competence as the primary goal of language instruction, this article represents an overt attempt to combine these disparate approaches in a common theme, that is, proposing WTC as the primary goal of language instruction.
Biodiversity Mapping in a Tropical West African Forest with Airborne Hyperspectral Data

Tropical forests are major repositories of biodiversity, but are fast disappearing as land is converted to agriculture. Decision-makers need to know which of the remaining forests to prioritize for conservation, but the only spatial information on forest biodiversity has, until recently, come from a sparse network of ground-based plots. Here we explore whether airborne hyperspectral imagery can be used to predict the alpha diversity of upper canopy trees in a West African forest. The abundance of tree species were collected from 64 plots (each 1250 m2 in size) within a Sierra Leonean national park, and Shannon-Wiener biodiversity indices were calculated. An airborne spectrometer measured reflectances of 186 bands in the visible and near-infrared spectral range at 1 m2 resolution. The standard deviations of these reflectance values and their first-order derivatives were calculated for each plot from the c. 1250 pixels of hyperspectral information within them. Shannon-Wiener indices were then predicted from these plot-based reflectance statistics using a machine-learning algorithm (Random Forest). The regression model fitted the data well (pseudo-R2 = 84.9%), and we show that standard deviations of green-band reflectances and infra-red region derivatives had the strongest explanatory powers. Our work shows that airborne hyperspectral sensing can be very effective at mapping canopy tree diversity, because its high spatial resolution allows within-plot heterogeneity in reflectance to be characterized, making it an effective tool for monitoring forest biodiversity over large geographic scales.
Very Large Annotated Database of American English

To construct a data base (the "Penn Treebank") of written and transcribed spoken American English annotated with detailed grammatical structure. This data base will serve as a national resource, providing training material for a wide variety of approaches to automatic language acquisition, a reference standard for the rigorous evaluation of some components of natural language understanding systems, and a research tool for the investigation of the grammar of naturally spoken English.
Elastic impedance based facies classification using support vector machine and deep learning

Machine learning methods including support‐vector‐machine and deep learning are applied to facies classification problems using elastic impedances acquired from a Paleocene oil discovery in the UK Central North Sea. Both of the supervised learning approaches showed similar accuracy when predicting facies after the optimization of hyperparameters derived from well data. However, the results obtained by deep learning provided better correlation with available wells and more precise decision boundaries in cross‐plot space when compared to the support‐vector‐machine approach. Results from the support‐vector‐machine and deep learning classifications are compared against a simplified linear projection based classification and a Bayes‐based approach. Differences between the various facies classification methods are connected by not only their methodological differences but also human interactions connected to the selection of machine learning parameters. Despite the observed differences, machine learning applications, such as deep learning, have the potential to become standardized in the industry for the interpretation of amplitude versus offset cross‐plot problems, thus providing an automated facies classification approach.
Effect of Posterior Tibial Slope on Outcomes After Posterior Cruciate Ligament Reconstruction

Background: Increased posterior tibial slope (PTS) leads to a relative anterior translation of the tibia on the femur. This is thought to decrease the stress on posterior cruciate ligament (PCL) reconstruction (PCLR) grafts. Purpose/Hypothesis: The purpose of this study was to analyze the effect of PTS on knee laxity, graft failure, and patient-reported outcome (PRO) scores after PCLR without concomitant anterior cruciate ligament reconstruction (ACLR). It was hypothesized that patients with higher PTS would have less knee laxity, fewer graft failures, and better PROs compared with patients with lower PTS. Study Design: Case-control study; Level of evidence, 3. Methods: All patients who underwent PCLR between 2001 and 2020 at a single institution were identified. Patients were excluded if they underwent concomitant or prior ACLR or proximal tibial osteotomy, were younger than 18 years, had <2 years of in-person clinical follow-up, and did not have documented PRO scores (Lysholm score and International Knee Documentation Committee [IKDC] score). Data were collected retrospectively from a prospectively gathered database. PTS measurements were recorded from perioperative lateral knee radiographs. A linear regression model was created to analyze PTS in relation to PRO scores. Patients with a grade 1 (1-5 mm) or higher posterior drawer were compared with those who had a negative posterior drawer. Results: A total of 37 knees met inclusion criterion; the mean age was 30.7 years at the time of surgery. The mean clinical follow-up was 5.8 years. No significant correlation was found between either the Lysholm score or the IKDC score and the PTS. Twelve knees (32.4%) had a positive posterior drawer at final follow-up. The mean PTS in knees with a positive posterior drawer was 6.2°, whereas that for knees with a negative posterior drawer was 8.3° (P = .08). No significant differences in PRO scores were identified for knees with versus knees without a positive posterior drawer. No documented graft failures or revisions were found. Conclusion: No significant differences were found in PROs or graft failure rates based on PTS at a mean of 5.8 years after PCLR. Increased tibial slope trended toward being protective against a positive posterior drawer, although this did not reach statistical significance.
Local Oxygen-Based Therapy (blue®m) for Treatment of Peri-Implant Disease: Clinical Case Presentation and Review of Literature about Conventional Local Adjunct Therapies

Peri-implant diseases including peri-implant mucositis and peri-implantitis are among the major causes of failure of implant-supported dental restorations. They are characterized by progressive inflammation of the peri-implant mucosa, extending to the surrounding connective tissues and leading to bone loss and implant failure. Although strict oral hygiene practices help in preventing peri-implant diseases, plaque buildup around the implant restoration leads to chronic inflammation, due to the adherent bacterial biofilm. While mechanical debridement and non-surgical therapy to remove inflamed connective tissue (ICT) form the mainstay of treatment, additional local adjunctive therapies enhance clinical outcomes. Topical oxygen therapy is known to reduce inflammation, increase vascularity, and act as a bacteriostatic measure. The use of oxygen-based therapy (blue®m) products as a local adjunctive therapy for peri-implant mucositis and peri-implantitis can result in clinical outcomes similar to that of conventional local adjuncts such as chlorhexidine, antibiotics, and antibacterial agents. This report aims to present the clinical findings of patients with peri-implant mucositis and peri-implantitis, who were managed using local oxygen-based therapy as an adjunct to non-surgical therapy. In addition, a review of the literature about commonly used local adjuncts for peri-implant diseases has been included in the report to provide a means of comparison between conventional local adjunct therapy and topical oxygen-based therapy. Based on the reported findings and reviewed literature, local oxygen-based adjunct therapy was equally effective as conventionally used local adjuncts such as antibiotics, antibacterials, and probiotics, in treating patients with peri-implant diseases.
Computed Tomography Angiography for the Evaluation of Carotid Atherosclerotic Plaque: Correlation With Histopathology of Endarterectomy Specimens

Background and Purpose— The goal of this study was to determine the efficacy of CT angiography for the characterization of plaque morphology and composition in carotid atherosclerotic disease. Methods— Fifty-five patients undergoing carotid endarterectomy were imaged preoperatively with single-slice spiral CT angiography. One hundred sixty-five endarterectomy sections were examined histologically at selected levels through the distal common and proximal internal carotid arteries. Plaque density was measured (in Hounsfield units) on axial CT sections, and the presence or absence of ulceration was noted. These observations were compared with the histological findings at corresponding levels. Data were analyzed with 2-sample t tests and 1-way analysis of variance (ANOVA). Results— ANOVA testing showed a statistically significant decrease in CT attenuation values with increasing plaque lipid but with a very high standard deviation of values. No other histological factor showed a statistically significant link with CT attenuation. Plaque ulceration was detected by CT with a sensitivity of 60% and a specificity of 74%. Conclusions— Analysis of plaque attenuation with single-slice spiral CT does not give useful information concerning plaque composition. The predictive value of CT for the detection of plaque ulceration was moderate. Single-slice CT angiography is insufficiently robust to be a useful tool for the characterization of carotid plaque composition and morphology.
A Methodology for the Evaluation of Web Graph Models and a Test Case

Valid models of the WWW are important for creating WWW-like representations, upon which new algorithms and applications for searching, indexing, compression etc. can be tested, but also for predicting the evolution of the Web and the emergence of important new phenomena. In this study we introduce a validation process for Web-graph models and use it to analyze the behavior of the exponential growth copying model, which has been explicitly designed to model the WWW. We study the effect of individual parameters on its effectiveness, suggest appropriate parameter values for the creation of Web-like graphs and indicate inherent deficiencies of the model
Skinning arbitrary deformations

Matrix palette skinning (also known as skeletal subspace deformation) is a very popular real-time animation technique. So far, it has only been applied to the class of quasi-articulated objects, such as moving human or animal figures. In this paper, we demonstrate how to automatically construct skinning approximations of arbitrary precomputed animations, such as those of cloth or elastic materials. In contrast to previous approaches, our method is particularly well suited to input animations without rigid components. Our transformation fitting algorithm finds optimal skinning transformations (in a least-squares sense) and therefore achieves considerably higher accuracy for non-quasi-articulated objects than previous methods. This allows the advantages of skinned animations (e.g., efficient rendering, rest-pose editing and fast collision detection) to be exploited for arbitrary deformations.