Miracle-dz/UniFAR-FTUs huggingface dataset:
Targeting FOXP3 Tumor-Intrinsic Effects Using Adenoviral Vectors in Experimental Breast Cancer

The regulatory T cell master transcription factor, Forkhead box P3 (Foxp3), has been detected in cancer cells; however, its role in breast tumor pathogenesis remains controversial. Here we assessed Foxp3 tumor intrinsic effects in experimental breast cancer using a Foxp3 binder peptide (P60) that impairs Foxp3 nuclear translocation. Cisplatin upregulated Foxp3 expression in HER2+ and triple-negative breast cancer (TNBC) cells. Foxp3 inhibition with P60 enhanced chemosensitivity and reduced cell survival and migration in human and murine breast tumor cells. We also developed an adenoviral vector encoding P60 (Ad.P60) that efficiently transduced breast tumor cells, reduced cell viability and migration, and improved the cytotoxic response to cisplatin. Conditioned medium from transduced breast tumor cells contained lower levels of IL-10 and improved the activation of splenic lymphocytes. Intratumoral administration of Ad.P60 in breast-tumor-bearing mice significantly reduced tumor infiltration of Tregs, delayed tumor growth, and inhibited the development of spontaneous lung metastases. Our results suggest that Foxp3 exerts protumoral intrinsic effects in breast cancer cells and that gene-therapy-mediated blockade of Foxp3 could constitute a therapeutic strategy to improve the response of these tumors to standard treatment.
Tripartite Entanglement for Qubits and Qudit in Double Photoionization of Xenon Atom

Quantum entanglement holds the key to an information processing revolution. In this article, we study the entanglement properties of tripartite states of two electronic qubits and ionic qudit,without observing spin orbit interaction (SOI), produced by single-step double photoionization from Xeon atom following the absorption of a single photon. The dimension of the Hilbert space of the qudit depends upon the electronic state of the residual photoion Xe 2+ . In absence of SOI, Russel-Salunders coupling (L-S coupling) is applicable. As the estimations of entanglement, we consider Peres-Horodecki condition and negativity. In case of L-S coupling, all the properties of a qubit-qudit system can be predicted merely with the knowledge of the spins of the target atom, the residual photoion, emitted electrons and state of polarization of the incident photons.
Generalized Riemann problem for dispersive equations

We study the inertia type regularization of equations for barotropic fluids. This regularization is formally obtained as the Euler-Lagrange equations for a Lagrangian containing terms which are quadratic with respect to the material derivative of density. Such a regularization arises, in particular, in the modeling of waves in bubbly fluids as well as in the theory of water waves (Serre-Green-Naghdi equations). We show that such terms are not always regularizing. The solution can develop shocks even in the presence of dispersive terms. In particular, we found such a shock solution relating a constant state with a periodic wave train. The shock speed coincides necessarily with the velocity of the corresponding wave train. The associated Rankine-Hugoniot relations correspond to Whitham's equations (modulation equations) of the regularized system. The numerical evidence of the existence of such shocks is demonstrated. To this aim, a robust high-order accurate numerical has been designed based on an appropriate operator splitting of the governing equations. In particular, it has been shown that such waves can dynamically be formed. Also, when such a wave is used for initial data, it is not destroyed by small perturbations. This proves a certain stability of these waves.
Atrial Fibrillation and Reperfusion Therapy in Acute Ischaemic Stroke Patients: Prevalence and Outcomes—A Comprehensive Systematic Review and Meta-Analysis

Atrial fibrillation (AF) significantly contributes to acute ischaemic stroke (AIS), yet its precise influence on clinical outcomes post-intravenous thrombolysis (IVT) and post-endovascular thrombectomy (EVT) has remained elusive. Furthermore, the overall prevalence of AF in AIS patients undergoing reperfusion therapy has not been clearly determined. Employing random-effects meta-analyses, this research aimed to estimate the pooled prevalence of AF among AIS patients undergoing reperfusion therapy, while also examining the association between AF and clinical outcomes such as functional outcomes, symptomatic intracerebral haemorrhage (sICH) and mortality. Studies comparing AF and non-AF patient groups undergoing reperfusion therapy were identified and included following an extensive database search. Forty-nine studies (n = 66,887) were included. Among IVT patients, the prevalence of AF was 31% (Effect Size [ES] 0.31 [95%CI 0.28–0.35], p < 0.01), while in EVT patients, it reached 42% (ES 0.42 [95%CI 0.38–0.46], p < 0.01), and in bridging therapy (BT) patients, it stood at 36% (ES 0.36 [95%CI 0.28–0.43], p < 0.01). AF was associated with significantly lower odds of favourable 90-day functional outcomes post IVT (Odds Ratio [OR] 0.512 [95%CI 0.376–0.696], p < 0.001), but not post EVT (OR 0.826 [95%CI 0.651–1.049], p = 0.117). Our comprehensive meta-analysis highlights the varying prevalence of AF among different reperfusion therapies and its differential impact on patient outcomes. The highest pooled prevalence of AF was observed in EVT patients, followed by BT and IVT patients. Interestingly, our analysis revealed that AF was significantly associated with poorer clinical outcomes following IVT. Such an association was not observed following EVT.
Task fMRI studies investigating inhibitory control in patients with obsessive-compulsive disorder and eating disorders: A comparative meta-analysis

Abstract Background Obsessive-compulsive disorder (OCD) and eating disorders (EDs) share similarities in terms of clinical characteristics and deficits in inhibitory control. Objective To investigate whether inhibitory control could serve as a common behavioural phenotype between OCD and EDs and whether it might be underpinned by shared and/or distinct neural signatures. Method We performed a quantitative meta-analysis of brain function abnormalities during the inhibitory control task-based functional Magnetic Resonance Imaging (fMRI) scan across patients with OCD and EDs using seed-based d mapping (SDM). Results The meta-analysis included sixteen OCD fMRI studies and ten EDs fMRI studies. And findings revealed that patients with OCD showed hypoactivation relative to healthy controls and patients with EDs in the anterior cingulate cortex, while compared to healthy controls and patients with OCD, patients with EDs showed hypoactivation in the right insula. Conclusions Patients with OCD and EDs are inclined to exhibit impaired inhibitory control, which may be attributed to different abnormal patterns of neural activation.
Isolation, identification and antibiogram profile of Aeromonas hydrophila from broiler chickens in Mymensingh Sadar, Bangladesh

Investigation of Aeromonas hydrophila was conducted to assess the microbial quality of broiler chickens from July to November 2019. A total of 60 samples from 20 broiler chickens were collected from two different locations of Mymensingh Sadar: KR market, Bangladesh Agricultural University (BAU) and Shesh mor bazar (10 birds from each location). Samples included 20 skins, 20 legs and 20 breast samples from 20 broiler chickens. PCR was done for the specific detection of each isolate and finally antimicrobial susceptibility testing was performed to check sensitivity pattern of each isolate. Alkaline peptone water was used for processing and enrichment of the samples followed by inoculation onto Aeromonas selective agar supplemented with ampicillin for the isolation and identification of A. hydrophila. Out of these 60 samples, 27 isolates were confirmed as A. hydrophila through biochemical tests and PCR where 55.56% isolates were recovered from Shesh mor market and other 44.4% isolates from KR market, BAU. Source-wise analysis revealed that maximum isolates of A. hydrophila were recovered from skin (59.26 %) followed by leg (22.22 %) and breast samples (18.52 %). PCR test revealed that all 27 isolates were found carrying lip gene which is specific for A. hydrophila. Isolates of A. hydrophila were found sensitive to ciprofloxacin (92%), gentamycin (66%) and chloramphenicol (50%); intermediate against erythromycin (50%), tetracycline (50%) and imipenem (50%); resistant against co-trimoxazole (84%) and ampicillin (100%). From the present study, it was found that samples were considerably contaminated with Aeromonas hydrophila causing risks for public health. Necessary control actions should be taken in every steps of production, processing and marketing for mitigation of this contamination. Asian Australas. J. Food Saf. Secur. 2020, 4(1), 22-30
Systematic Review of Platelet-Rich Plasma for Low Back Pain

Background: Low back pain (LBP) has a high economic burden and is strongly related to the degenerative process of the spine, especially in the intervertebral disc and of the facet joints. Numerous treatment modalities have been proposed for the management of LBP, and the use of platelet-rich plasma (PRP) has emerged as an innovative therapeutic option for degenerative disease of the spine. The present study aims to evaluate the efficacy of PRP injections in managing low back pain. Methods: We conducted a systematic review in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) recommendations, a registered at PROSPERO Systematic Reviews Platform, under number CRD42021268491. The PubMed, Web of Science, and Scopus databases were searched to identify relevant articles, along with hand searching to identify gray literature articles, with no language restrictions. Randomized clinical trials (RCTs), nonrandomized trials (NRTs), and case series (CSs) with more than 10 patients were considered eligible. The quality assessment and the risk of bias of the randomized clinical trials were evaluated using the RoB II tool. An evaluation of the description of the preparation methods was performed using an adapted version of the MIBO checklist. Results: An electronic database search resulted in 2324 articles, and after the exclusion of noneligible articles, 13 RCTs and 27 NRTs or CSs were analyzed. Of the 13 RCTs, 11 found favorable results in comparison to the control group in pain and disability, one showed no superiority to the control group, and one was discontinued because of the lack of therapeutic effect at eight-week evaluation. Description of the PRP preparation techniques were found in almost all papers. The overall risk of bias was considered high in 2 papers and low in 11. An adapted MIBO checklist showed a 72.7% compliance rate in the selected areas. Conclusions: In this systematic review, we analyzed articles from English, Spanish and Russian language, from large databases and grey literature. PRP was in general an effective and safe treatment for degenerative LPB. Positive results were found in almost studies, a small number of adverse events were related, the risk of bias of the RCTs was low. Based on the evaluation of the included studies, we graded as level II the quality of the evidence supporting the use of PRP in LBP. Large-scale, multicenter RCTs are still needed to confirm these findings.
Fresh-State and Mechanical Properties of High-Performance Self-Compacting Concrete with Recycled Aggregates from the Precast Industry

The urgent need to change the less positive impacts of the construction industry on the environment, and more specifically the production and use of concrete, is the main motivation for the research for more efficient and environmentally sustainable solutions. This paper presented the results of an experimental campaign whose ultimate goal was to produce high-performance self-compacting concrete (SCC) using recycled aggregates (RA) from the precast industry. The results of the fresh-state and mechanical properties tests performed on six concrete mixes (using RA from the precast industry) were presented. The first concrete mix is a reference mix using natural aggregates only (100% NA), and the remaining five mixes had various contents of fine (FRA) and coarse (CRA) recycled aggregates in concrete’s composition: (2) 25/25% (25% RA); (3) 50/50% (50% RA); (4) 100/100% (100% RA); (5) 0/100% (100% CRA); (6) 100/0% (100% FRA). The results showed that the high-performance concrete mixes with RA from the precast industry performed worse than the reference mix. However, taking into account all the mechanical properties studied, it can be concluded that RA from precast concrete elements are of very good quality and can be incorporated in the production of high-performance SCC. The potential demonstrated by the combined use of fine and coarse recycled aggregates was also emphasized. This type of work is expected to effectively contribute to raise awareness among the various players in the construction industry, particularly in the precast concrete industry, to the feasibility of using RA in significant quantities (notably coarse aggregates) and to the safety needed to assume structural functions, even for applications where high performance is required.
Bacterial Isolates and Their Antimicrobial Susceptibility Profile in Osteomyelitis Patients: An Experience From a Tertiary Care Center in a Hilly Area of Uttarakhand

Osteomyelitis, an infection related to bone and bone marrow, is very diverse in its pathophysiology and clinical presentation; hence, it is considered one of the most difficult-to-treat infections. The present study is aimed at assessing the microbiological profile of osteomyelitis and related antimicrobial susceptibility patterns in patients attending a tertiary care center in Uttarakhand, India, over a period of one year (January to December 2019). In aerobic culture, 58/105 (55.2%) bacterial isolates were detected. In addition, Staphylococcus aureus was the most common isolate, and amongst Gram-negative bacilli, most isolates that grew on culture were Escherichia coli (22.4%). Out of 21 S. aureus isolates, methicillin resistance was detected in nine [9/21 (42%)] cases, which is a matter of concern. Hence, proper training and application of antimicrobial stewardship are the need of the hour so that clinicians can initiate targeted therapy as early as possible.
MANILAS’ LUXURY HOTELS USING SERVQUAL FRAMEWORK: COMPREHENSIVE ASSESSMENT FROM ONLINE TRAVEL AGENCIES’ REPORTS

Online reviews are essential in the travel and hotel businesses. Because there are various types of hotels, customers frequently read online hotel reviews before making a reservation. The ability to distinguish between positive and negative reviews at luxury hotels according to SERVQUAL attributes of tangibility, reliability, empathy, responsiveness, and assurance could help hotels identify problems, prioritize solutions, and offer better services to Luxury hotels. The study outcomes will assist hotel management in gaining complete knowledge of client complaints regarding service difficulties and promoting service enhancements. This study was conducted to examine and analyze the content of positive and negative customer reviews from Booking.com, Expedia, and TripAdvisor in five-star hotels in Manila. SERVQUAL dimensions were used to classify positive and negative reviews from the selected OTAs in five-star hotels in Manila. Findings revealed that the responsiveness dimension in the recreational services area received the highest number of customer reviews than any other service area of the hotels gathered from the selected OTAs of the study.
Sex Differences among Overweight/Obese Kidney Transplant Recipients Requiring Oxygen Support Amid the COVID-19 Pandemic

Background and Objectives: Overweight/obesity puts individuals at greater risk for COVID-19 progression and mortality. We aimed to evaluate the impact of overweight/obesity on oxygen (O2) requirement outcomes of male and female kidney transplant recipients (KTRs) during the COVID-19 pandemic. Materials and Methods: We conducted a retrospective analysis of a cohort of KTRs diagnosed with COVID-19. Participants were stratified based on BMI categories, and data on the need for O2 therapy outcome were collected and analyzed separately for male and female KTRs. Results: In total, 284 KTRs (97 males and 187 females) were included in the study. Overweight/obesity was observed in 60.6% of male KTRs and 71% of female KTRs. Strikingly, overweight/obese women had a significantly higher requirement for supplemental O2 (63.3% vs. 41.7%, OR = 2.45, p = 0.03), particularly among older individuals (OR = 1.05, p = 0.04), smokers (OR = 4.55, p = 0.03), those with elevated lactate dehydrogenase (LDH) levels (OR = 1.01, p = 0.006), and those with lower admission and basal estimated glomerular filtration rate (eGFR) levels. Within this cohort, the necessity for O2 supplementation was correlated with more unfavorable outcomes. These included heightened mortality rates, transfers to the intensive care unit, employment of invasive mechanical ventilation, and the emergence of acute kidney injury requiring hemodialysis. On the other hand, although overweight/obese male KTRs had a higher prevalence of hypertension and higher fasting blood glucose levels, no significant association was found with COVID-19-related outcomes when compared to lean male KTRs. Conclusions: Overweight/obesity is highly prevalent in KTRs, and overweight/obese women demonstrated a higher need for supplemental O2. Therefore, the early identification of factors that predict a worse outcome in overweight/obese female KTRs affected by COVID-19 contributes to risk stratification and guides therapeutic decisions.
Stromal Vascular Fraction and Autologous Activated Platelet-Rich Plasma Combination in Treatment of Type 2 Diabetes: A Single Center Retrospective Study

Objective: A cutting edge potential treatment of type 2 diabetes (T2D) is the use of cellular therapy. One recent method is the use of combination of stromal vascular fraction (SVF) and autologous activated platelet-rich plasma (aaPRP). This study is aimed to evaluate the efficacy of SVF combined with aaPRP to treat T2D. Materials and methods: Patients with T2D who un - derwent SVF and aaPRP treatment were recruited in this study. The lipoaspirate was digested by H-Remedy enzyme and centrifuged to isolate SVF. Blood was drawn from the patients and the aaPRP was isolated. The combination of autologous SVF and aaPRP was administered intravenously to each patient. Patients’ 1-month and 3-month post-therapy HbA1c level were statistically analyzed to their baseline level. Results: A total of 39 patients, 28 male and 11 female, with T2D with mean age of 59±11 years and baseline HbA1c of 8.31 g/dL were included in this study. Com - bination of SVF and aaPRP treatment in T2D resulted in a statistically significant decrease in HbA1c level to 7.58 g/dL at 1 month and to 7.63 g/dL at 3 months post-therapy, while a decrease in HbA1c was still observed at 6 and 12 months after therapy. Conclusions: SVF combined with aaPRP may have potential to reduce HbA1c levels in patients with T2D. Further research is needed to confirm the findings of our research. (Clin Diabetol 2023; 12; 4: 247–252) Clinical trial registration number: NCT05925829
3-Layer Neural Network Classifier With the Heterogeneous Hidden Layers

The ways to improve the performance of classifiers based on the bithreshold architecture are considered in the paper. The model of 3-layer neural network binary classifier is proposed whose first hidden layer consists of neural units of 3 kinds: bithreshold neurons, linear threshold units and winner-take-all neurons, and every neuron in the second layer has only 3 inputs with predefined weights. The synthesis algorithm for such networks is designed and estimations of its time complexity and the size of resulting network are presented. The simulation results demonstrate that the application of the new architecture in the classifier design significantly improves its generalization ability.
Review on the Impact of Milk Oligosaccharides on the Brain and Neurocognitive Development in Early Life

Milk Oligosaccharides (MOS), a group of complex carbohydrates found in human and bovine milk, have emerged as potential modulators of optimal brain development for early life. This review provides a comprehensive investigation of the impact of milk oligosaccharides on brain and neurocognitive development of early life by synthesizing current literature from preclinical models and human observational studies. The literature search was conducted in the PubMed search engine, and the inclusion eligibility was evaluated by three reviewers. Overall, we identified 26 articles for analysis. While the literature supports the crucial roles of fucosylated and sialylated milk oligosaccharides in learning, memory, executive functioning, and brain structural development, limitations were identified. In preclinical models, the supplementation of only the most abundant MOS might overlook the complexity of naturally occurring MOS compositions. Similarly, accurately quantifying MOS intake in human studies is challenging due to potential confounding effects such as formula feeding. Mechanistically, MOS is thought to impact neurodevelopment through modulation of the microbiota and enhancement of neuronal signaling. However, further advancement in our understanding necessitates clinical randomized-controlled trials to elucidate the specific mechanisms and long-term implications of milk oligosaccharides exposure. Understanding the interplay between milk oligosaccharides and cognition may contribute to early nutrition strategies for optimal cognitive outcomes in children.
Deep Transformer Network and CNN Model with About 200k Parameters to Classify P300 EEG Signal

This research gathers participants“ electroencephalographic (EEG) data to build classifiers that can decode users” mental states. Although deep learning models can incorporate domain-dependent feature extraction into the classifier design, the architecture selection process for BCIs is often based on domain expertise. This paper examines whether it can build reliable classifiers for interpreting P300 event-related potentials using a systematic model selection mixed with common deep learning architectures. We report the findings of a CNNTransformer network model with 50k and 200k parameters in particular. We systematically investigate if a hybrid architecture which is Transformer and CNN, can improve the classification performance of P300 datasets. Because we would like to know how to improve the model selection process for P300 signals data analysis. To help the readers understand that the best model selection strategy with more muscular models than the existing ones may make the lives of stroke patients more convenient. If our readers are targeting to know the modern muscular deep learning architectures to analyze their recorded P300 dataset, then we recommend reading this paper.