Miracle-dz/UniFAR-FTUs huggingface dataset:
Monosodium glutamate causes hepato-cardiac derangement in male rats

People in the fast-food era rely on pre-packaged foods and engage in limited physical activity, which leads to a shift in eating patterns. Monosodium glutamate (MSG), a dietary ingredient used in this sort of cuisine, has been found to be hazardous to both experimental animals and humans. The objective of this study was to explore at the unnecessary changes caused by consuming MSG in secret and exceeding the recommended dosage. Hence, we decided to evaluate the impact of MSG by using three different doses (200, 400, and 600 mg/kg body weight orally) for 28 days in rats. We uncovered that all three MSG dosages result in a rise in body weight, dyslipidemia, inflammatory response, and hepato-cardiac marker enzymes, all of which imply hepatic and cardiac toxicity. Furthermore, changes in redox status suggest oxidative stress, which was higher in all three MSG dosages although not as much as in the MSG-600 group when compared to control. Such effects eventually manifested themselves in tissue architecture of the liver and heart, resulting in severe hepato-cardiac derangement, but the degree of tissue damage was greater in the MSG-600 group. As a result, it is possible that MSG has a negative influence on the liver and heart. However, the MSG-600 group showed a substantial effect, indicating that MSG should not be used in food preparation. Therefore, the findings of the study may aid in the formulation of health-care strategies and serve as a warning to the general public regarding the use of MSG in daily diet.
Predicted vs measured posterior corneal astigmatism for toric intraocular lens calculations

The Barrett toric formula and its predicted PCA performed better with the Ks from the OCT biometer than with the topographer SimKs and a measured PCA value. Purpose: To evaluate the astigmatic correction obtained with a toric intraocular lens using the keratometric readings (Ks) from a swept-source optical coherence tomography (SS-OCT) biometer and the Barrett toric formula with its predicted posterior corneal astigmatism (PCA) value and to compare the results with those expected by using the OCT Ks and a measured PCA from a scheimpflug topographer and by using the SimKs and the measured PCA from the Scheimpflug topographer. Setting: Private practice, Lynwood, California. Design: Retrospective observational study. Methods: All measurements were performed by the SS-OCT biometer and the Scheimpflug topographer and using the Barrett toric formula. Results: We evaluated 122 eyes of 122 patients. The mean absolute errors in predicted residual astigmatism for the entire series were 0.41 ± 0.19 diopters (D) (0.00 to 0.85 D) using the OCT Ks and predicted PCA, 0.45 ± 0.25 D (0.00 to 1.01 D) using the OCT Ks and measured PCA, and 0.49 ± 0.25 D (0.00 to 1.30 D) using the SimKs and measured PCA. The statistically significant differences between the errors had a P value of . 062 for the entire series (n = 122), . 26 for the subgroup with against-the-rule astigmatism (n = 68), . 47 for the subgroup with oblique astigmatism (n = 11), and . 05 for the subgroup with with-the-rule astigmatism (n = 43). The percentage of eyes within ±0.50 D were 74% (n = 90), 71% (n = 87) and 64% (n = 78) (P = .13) and within ±0.75 D were 99% (n = 121), 95% (n = 116) and 84% (n = 102) (P < .001), respectively. Conclusions: The Barrett toric formula and its predicted PCA performed better with the OCT K readings than with the topographer SimKs and a measured PCA.
Psychosocial Impact of Coronal Access Scars in Craniosynostosis Procedures on Patients and Their Families

Supplemental Digital Content is available in the text Abstract The coronal incision is the mainstay for access in craniosynostosis surgery. Scarring is a common concern of parents whose children are offered an open procedure. To the author's knowledge, there are no previous studies looking at the psychosocial impact of scarring from coronal access incisions for craniosynostosis procedures. The author's study focused on patients undergoing procedures for nonsyndromic single-suture craniosynostosis. This study comprised 3 parts: worldwide survey regarding coronal access incisions for craniosynostosis surgery, questionnaire to determine the psychosocial impact of the scars on patients and their parents, and measurement of postoperative scars in craniosynostosis patients. Survey responses from 46 craniofacial centers worldwide revealed a zig-zag was the most commonly utilized incision. Seventy-two percent of survey responses reported problems with postoperative stretching of the scar; only 20% of centers reported formal data collection of whether families were affected by this. Psychology questionnaires revealed that the majority of patients and their parents were not bothered by the zig-zag coronal scars. Patient felt the scars were less noticeable than the parents. Parent perceptions improved with age and time postsurgery. Coronal access scars following craniosynostosis surgery appear to stretch more in the supra-auricular region compared with the midline. These findings are useful for the craniofacial multidisciplinary team to inform parents contemplating surgery and who may be concerned about the impact of the scar in the future.
Siglec-15 promotes progression of clear renal cell carcinoma

Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cell carcinoma, accounting for approximately 80% of cases. Although most patients in the early stages of ccRCC can be cured by surgery, ccRCC is often in the advanced stage when patients are diagnosed, with >30% of the patients presenting with distant metastases at the time of diagnosis. For ccRCC patients with distant metastases, the 5-year survival is only 12%. Immunotherapy selectively corrects tumor microenvironment (TME) immunity, which is of great significance for patients with advanced ccRCC.
Influence of MWCNTs on β-Phase PVDF and Triboelectric Properties

The surface of multiwalled carbon nanotubes (MWCNTs) was chemically modified using 1-pyrenebutyric acid (PBA) to improve its compatibility with polyvinylidene fluoride (PVDF). The carboxylic acid groups of the MWCNTs-PBA (PCNTs) provide a β-phase nucleation site to the fluorine of PVDF along their surface. The content of the β-phase crystalline structure of PVDF was found to be the highest at a concentration of 1.0 wt. % of PCNTs, and these PVDF-PCNTs composites were utilized as active layers in triboelectric devices. The maximum output voltage achieved was 16 volts at a concentration of 1.0 wt. % of PCNTs in the PVDF composites.
Association of Counseling and Psychotherapy on Retention in Medication for Addiction Treatment Within a Large Medicaid Population

Objectives Buprenorphine/naloxone is an effective medication for the treatment of opioid use disorder. Unlike methadone, which can only be dispensed in federally waived clinics and which must be combined with specific psychosocial treatment, buprenorphine can be dispensed by individual prescribers who have completed an 8-hour training program, with no requirement that patients receive concomitant psychotherapy. The objective of this study is to quantify the association of counseling and psychotherapy on retention in treatment. We also examine the effect of buprenorphine dosage on retention. Methods We examined a cohort of 4987 members of a not-for-profit managed care organization serving Medicaid members in 41 counties in Pennsylvania. This cohort was selected from all members who had a full year without any medication for opioid use disorder followed by initiation of treatment with buprenorphine/naloxone in 2016 to 2017 and who remained Medicaid eligible for at least 80% of the following 2 years. Outcomes were estimated using inverse probability weighted propensity scores. Results The addition of counseling and psychotherapy within the first 8 weeks of treatment was associated with greater total retention in treatment and there was a dose-response relationship. A 16 mg/d or greater dose of buprenorphine was also associated with greater retention. Conclusions These results provide support for an integrated approach to treating people with an opioid use disorder, through a combination of buprenorphine pharmacotherapy and targeted counseling and psychotherapy within the first 2 months of treatment.
Impact of Clinical Sepsis Phenotypes on Mortality and Fluid Status in Critically Ill Patients

ABSTRACT Background: Sepsis is associated with high rates of in-hospital mortality, despite being the focus of medical research and public health initiatives for several years. The primary objective of this study was to determine the influence of septic phenotypes on rates of in-hospital mortality throughout intensive care unit (ICU) admission. Patients and Methods: Retrospective, single-center cohort study. Medical ICU of an academic medical center. Medical ICU patients admitted between January 2016 and August 2019 with a “sepsis alert” were screened for admitting diagnosis of “sepsis” or “septic shock. ” Patients were classified into one of four clinical sepsis phenotypes: multi-organ failure (MOF), respiratory dysfunction (RD), neurologic dysfunction (ND), or other patients (OP). Results: An analysis of 320 patients was completed. In-hospital mortality was different between groups (P < 0.001). Patients with the MOF phenotype had the highest rate of mortality (48.4%), followed by the ND phenotype (39.7%), RD phenotype (20.8%), and OP phenotype (13.7%). There were differences in volume balances between phenotypes at 48 h (P = 0.001), 72 h (P < 0.001), and 96 h (P < 0.001) after hospital presentation, with the MOF and ND phenotypes having the largest volume balances at these time points. Ventilator-free days (P < 0.001) and ICU length of stay (LOS) (P = 0.030) were different between groups. There was no difference in hospital LOS (P = 0.479). Conclusions: This data supports the presence of marked intra-disease differences in septic patient presentation and correlation with clinical outcomes including mortality. Additionally, significantly more positive fluid balances were observed between survivors and non-survivors in some patient subsets. Using pragmatic clinical variables readily available to providers to classify patients into septic phenotypes has the propensity to guide treatment strategies in the future.
O to bR transition in bacteriorhodopsin occurs through a proton hole mechanism

Significance Bacteriorhodopsin (bR) is a molecular ion pump that has been long regarded as a prototypical system that demonstrates the Grotthuss mechanism for long-range proton transport in biology. Using extensive quantum mechanical/molecular mechanical (QM/MM) simulations, we show that the O to ground state transition in bR, in fact, features proton exchange between two distant groups via a proton hole mechanism. The proton hole is stabilized by a highly conserved arginine residue, which also plays the crucial role of modulating the hydration level of the protein cavity via a conformational transition. Therefore, the current study highlights the general importance of protein internal hydration level change and consideration of all possible titration states of water to the mechanism of long-range proton transport in biomolecules. Extensive classical and quantum mechanical/molecular mechanical (QM/MM) molecular dynamics simulations are used to establish the structural features of the O state in bacteriorhodopsin (bR) and its conversion back to the bR ground state. The computed free energy surface is consistent with available experimental data for the kinetics and thermodynamics of the O to bR transition. The simulation results highlight the importance of the proton release group (PRG, consisting of Glu194/204) and the conserved arginine 82 in modulating the hydration level of the protein cavity. In particular, in the O state, deprotonation of the PRG and downward rotation of Arg82 lead to elevated hydration level and a continuous water network that connects the PRG to the protonated Asp85. Proton exchange through this water network is shown by ∼0.1-μs semiempirical QM/MM free energy simulations to occur through the generation and propagation of a proton hole, which is relayed by Asp212 and stabilized by Arg82. This mechanism provides an explanation for the observation that the D85S mutant of bacteriorhodopsin pumps chloride ions. The electrostatics–hydration coupling mechanism and the involvement of all titration states of water are likely applicable to many biomolecules involved in bioenergetic transduction.
Factors affecting Iranian nurses’ intention to leave or stay in the profession during the COVID‐19 pandemic

Abstract Aim This study was conducted to explore the factors affecting nurses’ intentions to leave or stay in their profession during the coronavirus pandemic in Iran. Introduction Because the effectiveness of a healthcare response to a disaster depends on an available, skilled, and motivated healthcare workforce, it is essential to understand and address potential barriers to and reasons for the intentions of medical staff to leave or stay in their profession. Methods A qualitative study with a conventional content analysis approach was conducted. The participants included nurses working in hospitals during the COVID‐19 pandemic, and nurses who had previously left their job or had been absent from work for a period of time. The participants were selected using a purposeful sampling strategy. Data were collected through 19 in‐depth, individual semi‐structured interviews with 16 nurses. The COnsolidated criteria for REporting Qualitative research checklist was used to report the study. Findings Three categories; commitment and work conscience (with a subcategory of risk‐taking), fear (with two subcategories of fear of family infection and fear of protective equipment shortages), and organizational factors (with two subcategories of organizational atmosphere of the hospital and motivational factors), emerged from the analysis. Conclusion The reasons for quitting a nursing job or to keep working as a nurse during the pandemic include both personal and organizational factors. Commitment and work conscience in pandemic conditions is one of the main factors for keeping nurses in their profession. Implication for nursing practice and policy Gaining insight into nurses’ understanding of the situation and perspectives is the key to being able to provide appropriate support and keep them in the workforce. Peer support can play an important role in supporting novice nurses in facing challenges posed by a pandemic and should be improved. Also, programs and strategies need to be planned to improve resilience among nurses and to help them to manage their stress and fear.
Bloodstream Infections at Two Neonatal Intensive Care Units in Ghana

Supplemental Digital Content is available in the text. Background: Bloodstream infections (BSIs) are a major cause of morbidity and mortality in hospitalized neonates. Data on antibiotic resistance in neonatal BSIs and their impact on clinical outcomes in Africa are limited. Methods: We conducted a prospective cohort study at 2 tertiary level neonatal intensive care units (NICUs) in Ghana. All neonates admitted to the NICUs were included from October 2017 to September 2019. We monitored BSI rates and analyzed the effect of BSI and antibiotic resistance on mortality and duration of hospitalization. Results: Of 5433 neonates included, 3514 had at least one blood culture performed and 355 had growth of a total of 368 pathogenic microorganisms. Overall incidence of BSI was 1.0 (0.9–1.1) per 100 person days. The predominant organisms were Klebsiella pneumoniae 49.7% (183/368) and Streptococcus spp. 10.6% (39/368). In addition, 512 coagulase negative Staphylococci were isolated but considered probable contaminants. Among K. pneumoniae, resistance to gentamicin and amikacin was 91.8% and 16.4%, respectively, while carbapenem resistance was 4.4%. All-cause mortality among enrolled neonates was 19.7% (1066/5416). The mortality rate was significantly higher in neonates with BSI compared with culture-negative neonates in univariate analysis (27.9%, n = 99/355 vs. 16.5%, n = 520/3148; hazard ratio 1.4, 95% confidence interval 1.07–1.70) but not in multivariate analysis. Conclusion: The diversity of etiologic agents and the high-risk of antibiotic resistance suggest that standard empirical treatment is unlikely to improve the outcome of BSIs in low and middle income. Such improvements will depend on access to reliable clinical microbiologic services.
The discrete-time Kermack–McKendrick model: A versatile and computationally attractive framework for modeling epidemics

Significance We demonstrate the strength of a general discrete-time modeling framework in which model specification is reduced to the bare essentials. The approach is due to Kermack and McKendrick, dating from 1927. Despite the many citations, the true contents of their work is essentially unknown. On top of that, their continuous-time model takes the mathematical form of a renewal equation, and only a few experts can handle such equations numerically. Here, we establish that the discrete-time version is equally general and flexible and, yet, is very simple to parametrize on the basis of data and to implement computationally. This makes the framework highly suitable for studying control scenarios for epidemic diseases such as COVID-19. The COVID-19 pandemic has led to numerous mathematical models for the spread of infection, the majority of which are large compartmental models that implicitly constrain the generation-time distribution. On the other hand, the continuous-time Kermack–McKendrick epidemic model of 1927 (KM27) allows an arbitrary generation-time distribution, but it suffers from the drawback that its numerical implementation is rather cumbersome. Here, we introduce a discrete-time version of KM27 that is as general and flexible, and yet is very easy to implement computationally. Thus, it promises to become a very powerful tool for exploring control scenarios for specific infectious diseases such as COVID-19. To demonstrate this potential, we investigate numerically how the incidence-peak size depends on model ingredients. We find that, with the same reproduction number and the same initial growth rate, compartmental models systematically predict lower peak sizes than models in which the latent and the infectious period have fixed duration.
Sulforaphane-Dependent Up-Regulation of NRF2 Activity Alleviates Both Systemic Inflammatory Response and Lung Injury After Hemorrhagic Shock/Resuscitation in Mice

ABSTRACT Hemorrhagic shock/resuscitation (HS/R) is closely associated with overwhelming oxidative stress and systemic inflammation. As an effective activator of the nuclear factor-erythroid factor 2 related factor 2 (Nrf2) pathway, sulforaphane (SFN) exerts antioxidant and anti-inflammatory effects. We explored SFN's effects on alveolar macrophages (AMs), systemic inflammation, and pulmonary damage in an isolated murine HS/R model. Male C57/BL6 wild type and transgenic antioxidant response element (ARE)-luciferase (luc) mice (both n = 6 per group) were exposed to either pressure-controlled HS/R (mean arterial pressure 35–45 mm Hg for 90 min) or sham procedure (surgery without HS/R) or were sacrificed without intervention (control group). Fluid resuscitation was performed via the reinfusion of withdrawn blood and 0.9% saline. Sulforaphane or 0.9% saline (vehicle) was administrated intraperitoneally. Mice were sacrificed 6, 24, or 72 h after resuscitation. Bioluminescence imaging of ARE-luc mice was conducted to measure pulmonary Nrf2 activity. Plasma was collected to determine systemic cytokine levels. Alveolar macrophages were isolated before measuring cytokines in the supernatant and performing immunofluorescence staining, as well as Western blot for intracellular Nrf2. Histological damage was assessed via the acute lung injury score and wet/dry ratio. Hemorrhagic shock/resuscitation was associated with pulmonary Nrf2 activation. Sulforaphane enhanced pulmonary Nrf2 activity and the Nrf2 activation of AM, while it decreased lung damage. Sulforaphane exerted down-regulatory effects on AM-generated and systemic pro-inflammatory mediators, while it did not have such effects on IL-10. In conclusion, SFN beneficially enhances pulmonary Nrf2 activity and promotes Nrf2 accumulation in AMs’ nuclei. This may exert not only local protective effects but also systemic effects via the down-regulation of pro-inflammatory cytokines. The administration of Nrf2 activator post-HS/R may represent an innovative treatment strategy.
A Novel Delay Estimation Method for Improving Corticomuscular Coherence in Continuous Synchronization Events

<italic>Objective</italic>: While the corticomuscularcoupling between motor cortex and muscle tissue has received considerable attention, which is typically quantitative measure to evaluate neural signals synchronization in the motor control system, little work has been published regarding the effect of underlying delay of two coupled physiological signals on coherence. <italic>Methods</italic>: In this study, we developed a novel delay estimation method, named rate of voxels change (RVC), detecting time delay in two coupled physiological signals. Based on RVC framework, delay compensation was used to adjust magnitude squared coherence (MSC) image. To illustrate the effectiveness of the RVC method, we compared the estimated delays and the adjusted MSC results based on RVC method and corticomuscular coherence with time lag (CMCTL) method. <italic>Results</italic>: The simulation results suggested that RVC method was not only superior to the CMCTL method in estimating different time delays, but also has better optimization effect on MSC image. The experimental results further confirmed that delay estimated by the proposed RVC method was more in line with the underlying physiology (controls: <inline-formula><tex-math notation="LaTeX">$22.8\ {{\mathbf ms}}$</tex-math></inline-formula> vs patients: <inline-formula><tex-math notation="LaTeX">$34.5\ {{\mathbf ms}}$</tex-math></inline-formula>). Meanwhile, RVC-based delay compensation could significantly optimize the MSC of specific regions. <italic>Significance</italic>: This study proved that RVC has remarkably higher reliability in detecting time delay between coupled neurophysiological signals, and the application of RVC was an improvement on the previous studies that mainly focused on biased MSC estimation.