Miracle-dz/UniFAR-FTUs huggingface dataset:
Prediction of the invasiveness of PTMC by a combination of ultrasound and the WNT10A gene

Objective The purpose of this study was to predict the invasiveness of papillary thyroid microcarcinoma (PTMC) via ultrasonography in combination with the Wnt family member 10A (WNT10A) gene to provide a reference basis for evaluating the invasive capability of PTMC. Methods Cancer tissue were collected from 182 patients with unifocal PTMC, and the patients were divided into the invasive group and the non-invasive group based on whether the lesions invaded the thyroid capsules or whether lymph node metastasis occurred. The expression of WNT10A protein was examined. Age, sex, maximum nodule diameter, color Doppler flow imaging (CDFI), nodule echo, microcalcification, aspect ratio, morphology (boundary), nodule location, internal structure, ultrasound-suspected lymph node metastasis (US-LNM), and WNT10A expression were compared between the invasive group and the non-invasive group. Univariate analysis and multivariate logistic regression analysis were performed, and a p value of less than 0.05 indicated that the difference was statistically significant. Results (1) 36 patients in the non-invasive group showed high expression and 66 patients showed low or no expression, while 54 patients in the invasive group showed high expression and 26 patients showed low or no expression, suggesting that the expression level of WNT10A was higher in the invasive group than in the non-invasive group, with a statistically significant difference between the two groups (P<0.01). (2) Univariate analysis showed that there were statistically significant differences between the invasive PTMC group and the non-invasive group in age, sex, maximum nodule diameter, microcalcification, US-LNM and high WNT10A expression. (3) Multivariate analysis showed that the risk factors for invasiveness in patients with PTMC included age < 45 years, maximum nodule diameter > 7 mm, microcalcification, US-LNM and high WNT10A expression. Conclusion The risk factors for PTMC invasiveness included age < 45 years, maximum nodule diameter >7 mm, microcalcification, US-LNM and high WNT10A expression. A combination of ultrasonography and WNT10A gene analysis could provide a reference basis for evaluating the invasive capability of PTMC.
Student achievement effects of technology-supported remediation of understanding of fractions

Students have difficulty learning fractions, and problems in understanding fractions persist into adulthood, with moderate to severe consequences for everyday and occupational decision-making. Remediation of student misconceptions is hampered by deficiencies in teachers’ knowledge of the discipline and pedagogical content knowledge. We theorized that a technology resource could provide the sequencing and scaffolding that teachers might have difficulty providing. Five sets of learning objects, called CLIPS (Critical Learning Instructional Paths Supports), were developed to provide remediation on fraction concepts. In this article, we describe one stage in a research program to develop, implement and evaluate CLIPS. Two studies were conducted. In Study 1, 14 grade 7–10 classrooms were randomly assigned, within schools, to early and late treatment conditions. A pre-post, delayed treatment design found that CLIPS had no effect on achievement for the Early Treatment group due to unforeseen implementation problems. These hardware and software issues were mitigated in the late treatment in which CLIPS contributed to student achievement (Cohen's d = 0.30). Study 2 was a pre-post, single group replication involving 18 grade 7 classrooms. The independent variable was the number of CLIPS completed. Completion of all five CLIPS contributed to higher student achievement: Cohen's d = 0.53, compared to students who completed none (d = 0.00) or 1–4 CLIPS (d = 0.02). The two studies indicate that a research-based set of learning objects is effective when the full program is implemented. Incomplete sequences deprive students of instruction in one or more constructs linked to other key ideas in the conceptual map and reduce the amount of practice required to remediate student misconceptions.
Circulating brain-derived extracellular vesicles expressing neuroinflammatory markers are associated with HIV-related neurocognitive impairment

Background Neurocognitive impairment remains prevalent in people with HIV (PWH) despite long term virological suppression by antiretroviral therapy (ART) regimens. Systemic and neuro-inflammatory processes are suggested to contribute to the complex pathology leading to cognitive impairment in this population, yet the underlying mechanisms remain unresolved. Extracellular vesicles (EVs) play a central role in intracellular communication and have emerged as key modulators of immunological and inflammatory responses. In this report, we examined the impact of EVs in PWH experiencing cognitive deficits to determine their relevance in HIV associated neuropathology. Methods EV phenotypes were measured in plasma samples from 108 PWH with either cognitive impairment (CI, n=92) or normal cognition (NC, n=16) by flow cytometry. Matched cerebrospinal fluid (CSF)-derived EVs were similarly profiled from a subgroup of 84 individuals who underwent a lumbar puncture. Peripheral blood mononuclear cells were assayed by flow cytometry to measure monocyte frequencies in a subset of 32 individuals. Results Plasma-EVs expressing CD14, CD16, CD192, C195, and GFAP were significantly higher in HIV-infected individuals with cognitive impairment compared to individuals with normal cognition. Increased CSF-EVs expressing GFAP and CD200 were found in the cognitive impairment group compared to the normal cognition group. Frequencies of patrolling monocytes correlated with plasma-EVs expressing CD14, CD66b, MCSF, MAP2, and GFAP. Frequencies of CD195 expression on monocytes correlated positively with plasma-EVs expressing CD41a, CD62P, and CD63. Expression of CD163 on monocytes correlated positively with CSF-EVs expressing GFAP and CD200. Finally, the expression of CD192 on total monocytes correlated with CSF-EVs expressing CD200, CD62P, and CD63. Conclusions EVs expressing monocyte activation and neuronal markers associated with HIV associated cognitive impairment, suggesting that distinct EV subsets may serve as novel biomarkers of neuronal injury in HIV infection. Further circulating platelet EV levels were linked to monocyte activation indicating a potential novel interaction in the pathogenesis of HIV-related cognitive impairment.
Effectiveness of road safety prevention in schools

The World Health Organization estimates that each year, 1.3 million people are killed and more than 50 million people worldwide are injured in road traffic accidents. According to a study conducted by the Allianz Center for Technology, more young people between the ages of 15 and 29 die in traffic accidents than as a result of illness, drugs, suicide, violence, or war events worldwide. That is about 400,000 per year, globally. Worldwide traffic accident prevention campaigns demonstrate the consequences of traffic accidents in an emotionalizing way in order to encourage drivers to adopt conscious safety behavior via adequate driving behavior. The consequences of traffic accidents are demonstrated by prevention campaigns often in an emotionalizing way to encourage drivers to adopt safety measurements through adequate driving behavior. Prior research suggests that the emotionalizing effect of the appeals must be accompanied by solution- as well as action-oriented and self-confidence-increasing measures, so that the instructive message is reinforced and does not lead to reactance. Thus, a strong need arose in the schools for a targeted training as the follow-up of emotional prevention campaigns. A suitable training for knowledge acquisition and knowledge transfer into everyday life was developed by means of the design-based research method. To create the targeted follow-up, various methods from cognitive behavioral therapy and common traffic safety programs were adapted. This publication is dedicated to a first explorative research approach in a non-standardized form of a social training. It approaches the question of negative emotional states immediately after a Crash Kurs NRW stage event, which is a prevention program in Germany that targets upper middle school and high school courses and originates from North Rhine-Westphalia. Changes in social behavior and development of participants' own norms, values, and attitudes were observed and documented and are discussed and presented in this article. The result of the survey confirmed prior research and showed visible effects of reactance after the Crash Curs NRW campaign. It was found that a structured follow-up training is suitable to gain reactive behavior from the stage event. Knowledge deficits about the cause and outcome of accidents were successfully addressed in the follow-up. This may have influence the reactance behavior and could be a key factor for successful prevention campaigns. Further publications will observe the connection between knowledge and reactance in subsequent iterative passes of modified follow-ups for the Crash Course NRW Campaign.
Populism in Peru: Populist attitudes and perception of the populist offer and its relationship with political cynicism and attitudes toward democracy

Populism is a phenomenon that is gaining attention in Political Psychology. The goal of the current study was to determine the relationship between populist attitudes, based on the populist demand and the perception of the populist offer, and several indices of political cynicism and attitudes toward democracy in Peru. To do this, a quantitative correlational study including 391 participants from diverse Peruvian locations was carried out. Both populist attitudes and critical perception of the populist offer are found to be directly related to Political Cynicism in its dimensions of Political Distrust, Political Hopelessness, and Political Moral Laxity, and inversely related to the dimension of Political System Change. Similarly, both dimensions of populism are directly related to Democratic Support and inversely related to Democratic Satisfaction. The findings support the notion that populist attitudes emerge in the context of distrust of the system and express an ambivalent relationship with democracy. Furthermore, the various approaches developed by the social sciences to address the populist phenomenon are discussed in terms of their strengths and limitations.
A nomogram for predicting the HER2 status of circulating tumor cells and survival analysis in HER2-negative breast cancer

Background In breast cancer patients with HER2-negative tumors (tHER2-), HER2-positive CTCs (cHER2+) were associated with promising efficacy of HER2-targeted therapy, but controversy has persisted over its prognostic effect. We developed a model including clinicopathologic parameters/blood test variables to predict cHER2 status and evaluated the prognostic value of cHER2+ in tHER2- patients. Methods cHER2+ was detected, blood test results and clinicopathological characteristics were combined, and a nomogram was constructed to predict cHER2 status in tHER2- patients according to logistic regression analysis. The nomogram was evaluated by C-index values and calibration curve. Kaplan–Meier curves, log-rank tests, and Cox regression analyses were performed to evaluate the prognostic value of cHER2 status. Results TNM stage, white blood cells (WBCs), neutrophils (NEUs), uric acid (UA), De Ritis ratio [aspartate transaminase (AST)/alanine transaminase (ALT)], and high-density lipoprotein (HDL) were found to be associated with cHER2 status in tHER2- patients in univariate logistic regression analysis, in which UA and De Ritis ratio remained significant in multivariate logistic regression analysis. A model combining these six variables was constructed, the C-index was 0.745 (95% CI: 0.630–0.860), and the calibration curve presented a perfect predictive consistency. In survival analysis, patients of the subgroups “with cHER2+/UA-low” (p = 0.015) and “with cHER2+/De Ritis ratio – high” (p = 0.006) had a significantly decreased disease-free survival (DFS). Conclusions Our nomogram, based on TNM stage, WBC, NEU, UA, De Ritis ratio, and HDL, may excellently predict the cHER2 status of tHER2- patients. Incorporation with UA and De Ritis ratio may enhance the prognostic value of cHER2 status.
TGF-β: A novel predictor and target for anti-PD-1/PD-L1 therapy

Transforming growth factor-β (TGF-β) signaling regulates multiple physiological processes, such as cell proliferation, differentiation, immune homeostasis, and wound healing. Besides, TGF-β plays a vital role in diseases, including cancer. Accumulating evidence indicates that TGF-β controls the composition and behavior of immune components in the tumor microenvironment (TME). Advanced cancers leverage TGF-β to reshape the TME and escape immune surveillance. TGF-β-mediated immune evasion is an unfavorable factor for cancer immunotherapy, especially immune checkpoint inhibitors (ICI). Numerous preclinical and clinical studies have demonstrated that hyperactive TGF-β signaling is closely associated with ICI resistance. It has been validated that TGF-β blockade synergizes with ICI and overcomes treatment resistance. TGF-β-targeted therapies, including trap and bispecific antibodies, have shown immense potential for cancer immunotherapy. In this review, we summarized the predictive value of TGF-β signaling and the prospects of TGF-β-targeted therapies for cancer immunotherapy.
Observational study of reactive ions and radicals in PDRs

We have carried out a survey of reactive ions (CO + , HOC + , HC 1 8 O + , SO + ) and cyclopropenylidene (C 3 H 2 ) in three prototypical photodissociation regions (PDRs), the reflection nebula NGC 7023, the Orion Bar and the planetary nebula (PN) NGC 7027. The reactive ion CO + has been detected in all the targets with fractional abundances ranging from ∼ 10 - 1 1 to ∼a few 10 - 9 . Its spatial distribution in NGC 7023 and the Orion Bar show that CO + arises in the innermost part (A, < 2 mag) of the PDR. In NGC 7027, the CO + lines shows an expansion velocity higher than that of the CO lines. This high expansion velocity is consistent with the CO + emission arising in the high velocity layer of neutral gas which is being accelerated by the ionized gas. Photochemistry determines the chemical composition of this layer. The reactive ions HOC + and SO + have been detected in NGC 7023 and the Orion Bar. In both sources, the fractional abundance of HOC + is enhanced by a factor of ∼ 10 towards the PDRs, with typical abundances, X H O C + = 0.7-3 x 10 - 1 1 . This enhancement produces a decrease of the [HCO + ]/[HOC + ] abundance ratio towards the PDR. In fact, we have derived [HCO + ]/[HOC + ] ∼ 50-120 in NGC 7023, which is the lowest ratio measured thus far. HOC + and SO + have not been detected in NGC 7027. Interestingly, this is the source with the highest CO + abundance, X C O + = 5 × 10 - 9 . This lack of detection is interpreted as due to the peculiar chemistry of C-rich PNs, in which the abundance of oxygenated molecules, in particular H 2 O, is low. We have detected cyclopropenylidene (C 3 H 2 ) in the three target PDRs. Similarly to the reactive ions, the abundance of C 3 H 2 in NGC 7023 and the Orion Bar is a factor of 10-100 higher in the PDRs than in the foreground molecular cloud with peak values ranging from 10 - 1 0 to 10 - 9 . In NGC 7027, we measured the maximum C 3 H 2 abundance with a value of ∼10 - 8 . Similarly to the case of CO + , the high expansion velocities of the C 3 H 2 lines in NGC 7027 suggests that its emission arises in the neutral gas which is being accelerated by the ionized gas. Photodestruction of Polycyclic Aromatic Hydrocarbons (PAHs) is proposed to explain the enhanced C 3 H 2 abundance in these PDRs.
Thyroid function disorders and secondary cancer following haematopoietic stem cell transplantation in pediatrics: State of the art and practical recommendations for a risk-based follow-up

Thyroid disorders (TD) represent a remarkable share of all the late morbidities experienced following pediatric haematopoietic stem cell transplantation (HSCT), with long-term reported occurrence often exceeding 70%. In addition, the data collected on wide cohorts of survivors assessed longitudinally outlined a progressive increase in the cumulative incidence of TD as far as 30 years following transplantation. Accordingly, a life-long monitoring of thyroid health is warranted among patients exposed to HSCT in childhood, in order to early detect TD and undertake a prompt dedicated treatment. Although several national and international consortia have provided recommendations for the early detection of thyroid disorders among childhood cancer survivors exposed to radiotherapy and alkylating agents, no guidelines specifically and thoroughly focused on HSCT-related TD have been published to date. As stem cell transplantation has become the standard-of-care in a growing body of non-oncological conditions, this urge has become pivotal. To highlight the challenging issues specifically involving this cohort of patients and to provide clinicians with the proposal of a practical follow-up protocol, we reviewed published literature in the light of the shared experience of a multidisciplinary team of pediatric oncologists, transplantologists, pathologists and endocrinologists involved in the long-term care of HSCT survivors. As a final result, we hereby present the proposals of a practical and customized risk-based approach to tailor thyroid health follow-up based on HSCT-related detrimental factors.
Prevalence, antibiotic and heavy metal resistance of Vibrio spp. isolated from the clam Meretrix meretrix at different ages in Geligang, Liaohe estuary in China

Vibrio as one of the main pathogens of shellfish diseases can cause serious human seafoodborne gastroenteritis and even death. In this study, we analyzed the bacterial communities from the clam, and compared the resistance phenotypes and genotypes of Vibrio spp. from Meretrix meretrix at different growth stages. High-throughput sequencing analysis revealed the predominance of Proteobacteria (50%) in the bacterial community and Vibrio was one of the dominant genera in the clam hepatopancreas in the summer. Vibrio abundance in Meretrix meretrix positively correlated with the water temperature (p<0.05). A total of 73 Vibrio isolates from Meretrix meretrix were classified into 19 species and the dominant strains included V. mediterranei (19%) and V. harveyi (11%), V. algolyticus (10%), and V. parahaemolyticus (8%). The species and abundance of Vibrio spp. were the highest in the 3-year-old of Meretrix meretrix compared with clams of other ages in the summer. Among the 73 isolates, 68 Vibrio strains were resistant to other 15 antibiotics except for sulfamethoxazole-trimethoprim with 57 resistant phenotypes. The most prevalent resistance was toward clindamycin (76%), followed by amikacin (63%), ampicillin (62%), rifampicin (62%), vancomycin (57%), and amoxicillin (50%). The ARI values of Vibrio spp. in different ages ranged from 0.13 to 0.18, and ARI values of 3-year-old (ARI=0.18) clams are higher than that of other ages clam. Approximately 72% of the resistant isolates showed multidrug-resistant phenotypes with maximum resistance to 15 antibiotics. Tolerance to heavy metals including Cd, Zn, and Cu was detected in the majority of antibiotic resistant isolates. In addition to the co-resistance to the same class of antibiotics, resistance to cephalosporin (CFP, CEP, CZ) were significantly correlated with penicillins (AMP, AMC) (p< 0.01), tetracycline (p < 0.001), sulfanilamide (SXT) (p< 0.01) and quinolone (CIP) (p< 0.01). The heavy metal resistance genes copB and nccA were significantly correlated with the clindamycin resistance phenotype (p<0.01). This study revealed that the habitat of Meretrix meretrix is in low exposure to antibiotics, and a link between heavy metal resistance genes and antibiotic resistance.
The effects of antioxidants on knee osteoarthritis: A systematic review and meta-analysis

Objective Knee osteoarthritis (KOA) is one of the growing health problems with a considerable burden. With recent research on the possible effectiveness of antioxidants in the remission of KOA symptoms, a systematic review and meta-analysis was required to confirm this hypothesis. Design Literature studies were searched on the most comprehensive databases such as PubMed, International Scientific Indexing, and Scopus, with no language and time restrictions. On 17 July 2021, a search strategy was developed based on the roots of “osteoarthritis (OA)” and “antioxidants,” with no time or language limitations. As the primary outcome, pain was evaluated based on all indicators for evaluating pain [e.g., Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain scores, the visual analog scale (VAS), and the numerical rating scale (NRS)]. The symptoms and functions of KOA and quality of life (QOL) were also considered as secondary outcomes, each of which was measured and reported by the corresponding instrument in the studies. To measure the changes in pain, symptoms, and functions of participants, we included randomized controlled trials with a placebo control or other medical therapeutic interventions. Publication bias was assessed using Begg's funnel plot and Egger's regression test, which was deemed to be statistically significant at 0.1, and the results were checked by the trim-and-fill test. Results After refinement, data were extracted from 31 documents from 7,698 primary searched papers. Using the VAS as a reliable psychometric measuring instrument, the present study revealed that a significant difference in the characteristics of disease-related symptoms of patients with KOA was reached after antioxidant therapy (standardized mean difference (SMD): 0.467, 95% confidence interval (CI): 0.303–0.632, p < 0.0001). The results reported by WOMAC confirmed no significant difference in the combined score, difficulty score, pain score, and stiffness score. Conclusion As the first comprehensive systematic review of the association between antioxidant supplementation and KOA, this study showed that antioxidants can decrease disease-related symptoms in patients with KOA. The results can be useful for health policy decisions and future related studies. Systematic review registration https://www.crd. york.ac. uk/prospero/display_record. php? ID=CRD42022351060, identifier: CRD42022351060.