Miracle-dz/UniFAR-FTUs huggingface dataset:
Studies on canine secondary immune-mediated hemolytic anemia

Immune mediated Hemolytic anemia was diagnosed using canine specific coomb's test (consisting of anti-IgG, -IgM and -C3) in 4 cases, which were severely anemic and had revealed regenerative anemia and spherocytosis on blood smear examination. The age of dogs suffering from Immune-mediated hemolytic anemia ranged from 2 to 10 years with a mean age of 4.5 years and all were males. Complete blood count and clinical chemistry was done which revealed leukopenia, thrombocytopenia, hyperbilirubinemia and elevation of alkaline phosphatase, alanine amino transferase and aspartate amino transferase enzymes. All the four cases were secondary immune mediated hemolytic anemia as three of them were also diagnosed positive for Eherlichia canis and one for Leptospira based on dot-enzyme immune assay.
Eastern Ghana’s first neonatal intensive care unit: achievements and lessons learned at 6 months

Introduction: Ghana’s Volta Region is home to 10% of the country’s births and has high neonatal mortality rates, yet historically has had no specialized neonatal services. At Volta Regional Hospital (VRH), sick newborns were previously cared for alongside other infants in a general babies ward. Recently, a new neonatal intensive care unit (NICU) was established as a major component of an ongoing quality improvement effort to improve paediatric outcomes at VRH. Here, we describe progress, achievements, and lessons learned in the NICU’s initial 6 months of operation. Methods: Volta Regional Hospital, commissioned …
Galectin-3 expression in nodular diseases of thyroid

Background. Galectin-3 is a beta-galactoside-binding protein, that has been shown to be involved in many cell functions, such as adhesion, growth, proliferation, malignant transformation and apoptosis. The expression of galectin-3 is observed in many human tissues, but its most important feature is the fact, that it achieves significantly higher concentrations in neoplasmatic thyroid cells. Objectives. The objective of the study was to analyze the expression of galectin-3 in benign and malignant thyroid nodules. We also tried to estimate the correlations between the intensity of galectin-3 expression and staging and grading of thyroid malignancy. Material and methods. We studied 68 patients operated on for thyroid tumours at the 1 st Department of General, Gastrointestinal and Endocrine Surgery of the Wroclaw Medical University between 1999 and 2004. The material for immunohistochemical studies was obtained from the Institute of Pathologic Anatomy of the Wroclaw Medical University. Results. In 28 of the 32 (87.5%) cases of analysed benign thyroid nodules (24 nodular goiter and 4 follicular adenomas) we found them to be negative for galectin-3 expression. In the 4 remaining cases (12.5%) – all nodular goiters – galectin-3 was expressed strongly and diffusely. In case of malignant lesions, in papillary carcinomas (all 31 cases, 100%) we have found high levels of expression of galectin-3 in many areas of the carcinomatous cells, such as the cytoplasm, the nucleus and cell membranes. In 5 follicular carcinomas (100%) we have also observed galectin-3 positivity, but less strongly and diffusely. Positivity for galectin-3 was also observed in stromal vessels of benign and malignant lesions as well as in areas of retrograde changes within thyroid follicles. Conclusions. 1. Basing upon our results we may conclude that galectin-3 immunohistochemical reaction seems to be a reliable tool in the diagnosis of papillary carcinomas and in distinguishing between nodular goiters and papillary carcinomas, i.e. the most frequent lesions. 2. Papillary carcinomas show a 100% expression of galectin-3. 3. Nodular goiters show variable positivity for galectin-3. 4. In follicular carcinomas the immunohistochemistry of galectin-3 is weaker.
Mupirocin resistance in clinical isolates of methicillin-resistant Staphylococcus aureus from a tertiary care rural hospital

Background and Aims: Mupirocin is a topical antibiotic that has been used extensively for treating methicillin-resistant Staphylococcus aureus (MRSA) associated infections. However, the prevalence of mupirocin-resistant MRSA has increased with the extensive and widespread use of this agent. The aim was to determine the rates of high-level and low-level mupirocin resistance in MRSA to study the antimicrobial resistance pattern and clindamycin resistance in mupirocin-resistant MRSA. Methods: A total of 267 non-duplicate clinical isolates of MRSA from various clinical specimens were tested for mupirocin resistance by the disk diffusion method using 5 and 200 μg mupirocin disks. MRSA isolates were tested for antibiotics by Kirby-Bauer disk diffusion method as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Erythromycin-resistant isolates of MRSA were further studied for inducible clindamycin resistance by "D test" as per CLSI guidelines. Results: Of 267 MRSA isolates, high-level mupirocin resistance was observed in 5.99% and low-level resistance in 15.35%. Mupirocin-resistant MRSA isolates showed higher antibiotic resistance to fusidic acid (14.03% vs 7.14%), rifampicin (5.26% vs 2.38%), erythromycin (68.42% vs 58.57%), and clindamycin (52.63% vs 45.71%). No MRSA strains were found to be resistant to vancomycin and linezolid. Mupirocin-resistant MRSA isolates showed higher constitutive macrolide-lincosamide-streptogamin B (cMLS B ; 51.28% vs 42.98%) and inducible macrolide-lincosamide-streptogamin B (iMLS B ; 17.94% vs 13.15%) resistance, as compared to mupirocin-sensitive MRSA isolates. Conclusion: The emergence of mupirocin resistance could be limited by regular surveillance and effective infection control initiatives so to inform health care facilities to guide therapeutic and prophylactic use of mupirocin.
Evaluation and comparison of camel milk with cow milk and buffalo milk for gross composition

The study was conducted to collect camel milk available nearby Anand-Kheda and Kutchh district and analyse for gross chemical composition. The average total solids (TS) content in camel (Anand and Kheda), camel (Kutchh), cow and buffalo milk was 11.97%, 9.95%, 13.07% and 15.57%, respectively. The average fat content in camel (Anand and Kheda), camel (Kutchh), cow and buffalo milk was 4.43%, 2.90%, 4.68% and 6.38%, respectively. The average solid not fat (SNF) content in camel (Anand and Kheda), camel (Kutchh), cow and buffalo milk was 7.56%, 7.04%, 8.39% and 8.97%, respectively; average protein content in camel (Anand ad Kheda), camel (Kutchh), cow and buffalo milk was 3.00%, 2.66%, 3.32% and 3.87%, respectively. The average lactose content in camel (Anand and Kheda), camel (Kutchh), cow and buffalo milk was 4.25%, 3.77%, 4.42% and 4.70% whereas ash content was 0.71%, 0.84%, 0.68% and 0.69%, respectively. Moreover, camel milk content higher amount of chloride and it was 0.20%, 0.25%, 0.11% and 0.12%, respectively.
A STUDY OF KNOWLEDGE, ATTITUDES AND PRACTICES (KAP) SURVEY OF FAMILIES TOWARD THEIR CHILDREN WITH INTELLECTUAL DISABILITY IN BARWANI, INDIA

The purpose of this study was to conduct an assessment of knowledge, attitudes and practices related to intellectual disability among families with such children in Barwani, India. A total of 41 parents (tribal - 28 & non tribal – 13) from 43 villages covering 55,366 population of two blocks of Barwani district were interviewed in their own languages in order to understand their knowledge, attitudes and practices toward their children. A questionnaire with 15 items comprising of major rehabilitation components (medical, educational, economical, social and behavioral) was used for the study. Data were analyzed and the results are discussed based on responses of tribal versus non-tribal and male versus female. Implications for practice are presented.
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIPYRETIC, PURGATIVE STUDIES OF JATROPHA GOSSYPIFOLIA

Jatropha gossypifolia (Euphorbiaceae) a common garden plant in tropical countries has been used as a traditional medicine. Plants are well known as a major source of modern medicines. From ancient times, humans have utilized plants for the treatment or prevention of diseases, leading to the dawn of traditional medicine. Jatropha gossypifolia is one of the plants that are used in Chinese, Ayurvedic and Thai traditional medicine for the treatment of fever, pain and dysentery. Preliminary phytochemical studies and Antipyretic, Purgative activities of ethyl alcohol and acetone extracts were investigated using albino rats of both the sexes. The phytochemical screening of ethanolic extracts of Jatropha gossypifolia leaves shows the presence secondary metabolites such as Saponin, Tannins, Phenols, Steroids, Glycosides, Flavonoids etc., The ethanolic and acetone extract of Jatropha gossypifolia have significant antipyretic activity and purgative activity. So the present study confirms the presence of valuable chemicals in Jatropha gossypifolia leaves and highlights the real potential of Jatropha gossypifolia leaves extracts in the preparation of versatile antipyretic and purgative drugs in future.
Study of Prevalence of Tibial Length Inequality on Both Sides

Leg length discrepancy is one of the conditions which can have a wide range of implications including back ache. One of the factors for leg length discrepancy is inequality / deformity of the skeletal elements or bones. Tibia being one of the bones in the lower limb length inequality of the length of tibea of the two sides needs evaluation. Present study is an attempt to study the prevalence of inequality of tibia from the cadaveric collection; Study was done on the collection of tibea in the Bone Bank of Dept of Anatomy, Govt. Medical College, Aurangabad. 100 pairs of human adult tibea of known sex and free from obvious deformity were used in the present study (70 males and 30 females) measurements were done using osteometer and statistically analysed Approximately 16% of the samples showed statistically significant inequality i.e. more than 5 mm length difference. Of these (18% of male samples and 10% of female samples). The findings are in corroboration with a number of previous workers. LLD prevalence gives an idea about the possibility of an important etiology in low back pain and hence should be emphatically considered for the evaluation of low back
Effect of Sequential Intrastromal Corneal Ring Segment Implantation and Corneal Collagen Crosslinking in Corneal Ectasia

Purpose To assess the clinical efficacy of sequential intrastromal corneal ring segment (ICRS) implantation and corneal crosslinking (CXL) in corneal ectasia. Methods This retrospective case series included eight eyes in which both ICRS implantation and CXL had been performed. CXL was performed within 1 month after ICRS implantation. The clinical outcomes (visual acuity, refractive errors, keratometry, and topographic indices) of these patients were compared with those of patients who had undergone only ICRS implantation (eight eyes) or CXL (20 eyes). Results Greater improvement in uncorrected visual acuity was observed in the ICRS + CXL group than in the ICRS or CXL alone groups at both 6 (p = 0.008) and 12 months (p = 0.028). Refractive errors of sphere and spherical equivalent were significantly reduced in both the ICRS (p = 0.002 at 6 months, p = 0.004 at 12 months) and ICRS + CXL groups (p < 0.001 at both 6 and 12 months). Keratometric values including the maximum, minimum, and average were significantly reduced in all 3 groups at postoperative 6 and 12 months; however, the greatest reductions were observed in the ICRS + CXL group (all p < 0.001). Conclusions ICRS implantation followed by CXL within 1 month seems to be effective, and may be superior to ICRS or CXL alone in improving visual acuity and reducing refractive errors and keratometric values.
Significance of PD-L1 expression in carbon-ion radiotherapy for uterine cervical adeno/adenosquamous carcinoma

Objective Programmed cell death-ligand 1 (PD-L1) is expressed in tumor cells and has been shown to predict clinical outcomes of several types of malignancies. The aim of this study was to investigate the effects of carbon-ion (C-ion) beam irradiation on PD-L1 expression in human uterine cervical adeno/adenosquamous carcinoma (UCAA) cells and clinical samples and to identify the prognostic factors for outcomes after C-ion radiotherapy (CIRT). Methods The effects of C-ion irradiation on PD-L1 expression in human UCAA and cervical squamous cell carcinoma cells were examined by flow cytometry. We examined PD-L1 expression in UCAA biopsy specimens from 33 patients before CIRT started (pre-CIRT) and after 12 Gy (relative biological effectiveness [RBE]) irradiation (post-12Gy-C) in 4 fractions of CIRT to investigate the correlation between PD-L1 status and clinical outcomes. Results The PD-L1 expression was upregulated by C-ion beam in a dose-dependent manner in HeLa and SiHa cells through phosphorylated Chk1. The overall frequencies of pre-CIRT and post-12Gy-C PD-L1 positivity were 45% (15/33) and 67% (22/33), respectively. The post-12Gy-C PD-L1 expression was significantly elevated compared to the pre-CIRT PD-L1 expression. There was no significant relationship between the pre-CIRT PD-L1 status and clinical outcomes, such as local control (LC), progression-free survival (PFS), and overall survival (OS). However, the post-12Gy-C PD-L1 expression had better correlation with PFS, but not with LC and OS. Conclusion CIRT can induce PD-L1 expression in UCAA and we propose that PD-L1 expression after starting CIRT may become as a predictive prognostic marker in CIRT for UCAA.
Green Consumer Behavior: The Case of Czech Consumers of Generation Y

The aim of this article is to reveal the perception of green marketing by young consumers from Generation Y and present a research model with variables that influence green consumer behavior (GCB). As the main method, we used a questionnaire survey with a sample of 520 respondents, mainly students at the University of Economics in Prague. Results were subjected to exploratory factor analysis, which examined convergent and discriminant validity. Finally, a model with four variables (Environmentalism, Preference, Payment, Tolerance) that influence GCB was confirmed. Recommendations of the research enable us to better understand the reasons why consumers from Generation Y do, or do not, buy green products. Firms can better focus their marketing strategies and communications with respect to variables that influence GCB. This research uncovers Generation Y consumers’ distinct preferences toward GCB. It also contributes to theoretical and practical aspects of GCB and has implications for research and practice, as green marketing continues to be an important issue. In terms of practical application in industry, the research’s outcomes are essential to promote better market demand, segmentation, targeting, and positioning of green products for Generation Y consumers.
Epidemiological Trends and Practices of Animal Bite Victims Presenting to the OPD, District & Medical College Hospital, India.

Introduction: Rabies, an invariably fatal viral disease, is transmitted to humans through animal bites, most commonly dogs. The disease is preventable through timely pre-and post-exposure vaccination. Methods: cross sectional institutional study was conducted in 406 animal bite victims animal bites victims presenting to the tertiary care hospital and district hospital Rewa, Madhya Pradesh. A pretested and structured oral questionnaire was used to elicit the required information regarding socio-demographic profile of animal bite victims, animal bite information & practices done after animal bites for prevention and control of rabies. Data was analyzed using graphpad software. Results: Among 406 victims 76.3% were males, 56.7% belongs to 15-45 yrs, 61.6% were living in urban area,77.1% educated upto primary level or more,31.3% and 21.7% of the victims were agriculture worker and laborers respectively . 95.8% victims bitten by dog, 89.4% had Category III bite, lower extremity was affected in 60.8%, 75.9% bites were unprovoked, fate of the animal was not known in 78.6% and 46% of the victims were affected during evening hours. 55.2% had taken home remedies immediately, 38.7% victims washed the animal bite site with water or soap with water before presenting to the hospital. 9.8% victims had taken ARV with in 24 hrs. Conclusions: Animal bite especially dog bite still continues to be a public health problem affecting urban male of productive age group involved in outdoor activities. Practices for wound care and management and preventive measures for rabies is found to be poor.
Immunohistochemical characterization of E48 and CD44-v6 expression in head and neck carcinomas

Contexte - L'objectif a ete d'etudier l'expression de deux antigenes de la surface cellulaire, E48 et CD44-v6, en tant que marqueurs pour caracteriser les cellules des cancers epidermoides (SCC) de la tete et du cou (HN) . L'etude est une contribution au developpement de la therapeutique immunostimulante post-operatoire. Materiel et Methodes - Des echantillons de tissu tumoral de 60 patients atteints de HNSCC operable ont ete etudiee immunohistochimiquement en utilisant la methode de l'APAAP (phosphatase alkaline-phosphatase anti-alkaline) avec les deux anticorps monoclonaux (MoAb) E48 (CENTOCOR B.V., Leiden / Pays Bas) et CD44-v6 (clone VFF-18, Bender, Autriche). Tous les specimens tumoraux provenaient de patients qui avaient ete chirurgicallement traites sans radiotherapie, ni chimiotherapie prealable. Nous avons determine le pourcentage et les intensites de coloration dans les cellules tumorales. Les resultats ont ete correles avec la differenciation histopathologique tumorale et avec la classification de l'UICC. Resultats - Tous les HNSCC etudies exprimaient les deux antigenes de surface. Cependant, la proportion de regions tumorales non reactives augmentait avec une differenciation decroissante. Les pourcentages de l'expression des antigenes E48 et CD44-v6 etaient differents dans les cellules SCC, et des intensites differentes de coloration ont ete observees. La regulation vers le bas de l'expression de ces deux antigenes avec differenciation tumorale decroissante semble caracteriser le phenotype du HNSCC. Conclusions - Les antigenes E48 et CD44-v6 sont tous deux fortement exprimes dans les tumeurs de grade 1 HNSCC et egalement partiellement dans les tumeurs G2, en particulier CD44-v6. Nous suggerons par consequent que les patients atteints de tumeurs G1 ou G2 sont des candidats pour une strategie d'immunostimulation post-operatoire comportant une vaccination active ou passive.
Changes in quality of mango-pineapple spiced beverage during storage

Mango-pineapple spiced beverages were prepared from ‘Dasheharl’ mango and ‘Kew’ pineapple. Fifteen per cent blended juices (85:15) were used for preparation of ready-to-serve (RTS) beverages having 10° Brlx, 0.2 % acidity and 0.006 % cardamom spice drops. The RTS beverages stored in white and amber coloured bottles for 6 months under 3 different storage environment viz., ambient temperature (12.5−3 6°C), cool chamber (10−29.6°C) and low temperature (4 ± 1°C) showed a gradual decrease in sensory quality, acidity, ascorbic acid and tannins. Retention of ascorbic acid was more in beverages stored in amber coloured bottles under low temperature. An increasing trend was found in total soluble solids, reducing sugars, total sugars and NEB. The hunter colour values ‘L and a’ got decreased over 6 months. The major volatile flavouring compound found in mango-pineapple spiced beverage was Terpenyl acetate that got decreased during storage.
Adverse Health Outcomes Among US Testicular Cancer Survivors After Cisplatin-Based Chemotherapy vs Surgical Management

Abstract We evaluated for the first time, to our knowledge, adverse health outcomes (AHOs) among US testicular cancer survivors (TCS) given chemotherapy (n = 381) vs surgery-only patients (n = 98) managed at a single institution, accounting for non-treatment-related risk factors to delineate chemotherapy’s impact. Chemotherapy consisted largely of bleomycin-etoposide-cisplatin (BEP) administered in three or four cycles (BEPx3, n = 235; BEPx4, n = 82). Incidence of at least 3 AHOs was lowest in surgery-only TCS and increased with BEPx3, BEPx4, and other cisplatin-based regimens (12.2%, 40.8%, 52.5%, 54.8%; P < .0001). Multivariable modeling assessed associations of risk factors and treatment with hearing impairment, tinnitus, peripheral neuropathy, and Raynaud phenomenon. Risk for each AHO statistically increased with both increasing chemotherapy burden (P < .0001) and selected modifiable risk factors (P < .05): hypertension (odds ratio [OR] = 2.40) and noise exposure (OR ≥ 2.3) for hearing impairment; noise exposure for tinnitus (OR ≥ 1.69); peripheral vascular disease for neuropathy (OR = 8.72); and current smoking for Raynaud phenomenon (OR = 2.41). Clinicians should manage modifiable risk factors for AHOs among TCS.
Involvement of adenosine A1 receptors in systemic inflammation and altered vascular reactivity in allergic mice

Involvement of Adenosine A1 receptors in systemic inflammation and altered vascular reactivity in allergic mice Dovenia S. Ponnoth Epidemiological studies have established that people who have lung diseases such as asthma and COPD are likely to develop adverse cardiovascular events. While the reason for this has not been elucidated, it is believed that inflammation could be the key component linking the two conditions. Adenosine, an endogenous purine nucleoside, has been strongly implicated in asthma pathophysiology and also has potent effects on the cardiovascular system. This work was undertaken to gain a better understanding of the relationship between asthma and its cardiovascular effects, and more specifically the possible role adenosine plays under these conditions. We hypothesize that asthmatic lung inflammation translates into systemic inflammation and alters vascular responses where adenosine plays an important role. Therefore, this study investigated the effects of aerosolized adenosine, used to elevate lung adenosine levels, on vascular reactivity and inflammation in a well established mouse model of allergic asthma established in our lab. We found that allergic mice had poor vasorelaxation to adenosine and systemic inflammation, in addition to lung inflammation, and aerosolized adenosine exacerbated these effects. Data indicated a possible role for A1 adenosine receptor (AR) in altered vascular reactivity and inflammation. Based on these findings, we next studied the effects of adenosine using genetically modified mice in which the A1 AR gene was deleted (A1KO) and corresponding A1 wild-type(A1WT) mice, in which the A1 receptor was present. Allergic A1KO mice had no systemic inflammation, and the adenosine-mediated vasorelaxation responses obtained were comparable to non-allergic mice. Aerosolized adenosine also did not have any effect in A1KO mice. On the other hand, allergic A1WT mice had lower aortic relaxation response to non-selective adenosine analog NECA, presence of significantly higher levels of inflammatory cytokines in plasma and higher airway responsiveness to NECA, compared to non-allergic A1WT controls and all groups of A1KO mice, with aerosolized adenosine exacerbating all these responses. From these data, it can be concluded that asthmatic mice had poor vascular responses and inflammation, possibly mediated through the A1 AR.