Miracle-dz/UniFAR-FTUs huggingface dataset:
Human iPSC-Derived Retinal Organoids and Retinal Pigment Epithelium for Novel Intronic RPGR Variant Assessment for Therapy Suitability

The RPGR gene encodes Retinitis Pigmentosa GTPase Regulator, a known interactor with ciliary proteins, which is involved in maintaining healthy photoreceptor cells. Variants in RPGR are the main contributor to X-linked rod-cone dystrophy (RCD), and RPGR gene therapy approaches are in clinical trials. Hence, elucidation of the pathogenicity of novel RPGR variants is important for a patient therapy opportunity. Here, we describe a novel intronic RPGR variant, c.1415 − 9A>G, in a patient with RCD, which was classified as a variant of uncertain significance according to current clinical diagnostic criteria. The variant lay several base pairs intronic to the canonical splice acceptor site, raising suspicion of an RPGR RNA splicing abnormality and consequent protein dysfunction. To investigate disease causation in an appropriate disease model, induced pluripotent stem cells were generated from patient fibroblasts and differentiated to retinal pigment epithelium (iPSC-RPE) and retinal organoids (iPSC-RO). Abnormal RNA splicing of RPGR was demonstrated in patient fibroblasts, iPSC-RPE and iPSC-ROs, leading to a predicted frameshift and premature stop codon. Decreased RPGR expression was demonstrated in these cell types, with a striking loss of RPGR localization at the ciliary transitional zone, critically in the photoreceptor cilium of the patient iPSC-ROs. Mislocalisation of rhodopsin staining was present in the patient’s iPSC-RO rod photoreceptor cells, along with an abnormality of L/M opsin staining affecting cone photoreceptor cells and increased photoreceptor apoptosis. Additionally, patient iPSC-ROs displayed an increase in F-actin expression that was consistent with an abnormal actin regulation phenotype. Collectively, these studies indicate that the splicing abnormality caused by the c.1415 − 9A>G variant has an impact on RPGR function. This work has enabled the reclassification of this variant to pathogenic, allowing the consideration of patients with this variant having access to gene therapy clinical trials. In addition, we have identified biomarkers of disease suitable for the interrogation of other RPGR variants of uncertain significance.
Power-Efficient Passive Beamforming and Resource Allocation for IRS-Aided WPCNs

This paper studies an intelligent reflecting surface (IRS)-assisted wireless-powered communication network (WPCN), where a hybrid access point (HAP) broadcasts energy signals to multiple devices for their energy harvesting in the downlink (DL) and then the devices use the harvested energy to transmit information signals to the HAP in the uplink (UL) with the help of an IRS. In particular, we propose three types of IRS beamforming configurations, namely fully dynamic IRS beamforming (FDBF), partially dynamic IRS beamforming (PDBF), and static IRS beamforming (SBF), to strike a balance between the system performance and signaling overhead as well as implementation complexity. Moreover, we adopt a practical non-linear energy harvesting (EH) model, and leverage a power-splitting (PS) EH receiver architecture with multiple rectifiers to avoid the input radio frequency power to get stuck into the saturation regime. We aim to minimize the transmit energy consumption at the HAP by jointly optimizing the DL/UL time allocation, the HAP/devices transmit power, the PS factor, and IRS phase shifts, subject to a set of minimum throughput requirements for individual devices. To address the resulting non-convex optimization problems, a successive convex approximation (SCA) based alternating optimization algorithm is proposed. Moreover, we study the case with the ideal linear EH model and two algorithms, namely SCA-based algorithm and semidefinite relaxation (SDR) algorithm, are proposed. Simulation results demonstrate the effectiveness of our proposed designs over various benchmark schemes and also unveil the importance of the joint design of IRS beamforming and PS rectifiers for achieving energy efficient WPCNs in practice.
The investigation of limiting factors of earnings management for companies’ listed Tehran stock exchange

The current study was to examine the limiting factors of earnings management for companies’ listed Tehran stock exchange. The study was a kind of applied, descriptive-correlative research. All listed companies in Tehran stock exchange were selected as statistical population during 2009 to 2013. So, data was gathered by library method and collected by systematic eliminated sampling method. The results show that there is a significant inverse relationship between board size and earnings management in companies’ listed Tehran stock exchange. The result doesn’t show any significant relationship between dividend and earnings management in companies’ listed Tehran stock exchange.
Psychological Vulnerability Associated With Stress Coping Strategies in Japanese University Athletes

This study examined the stress coping strategies of athletes with high psychological vulnerability. The participants were 487 university athletes (mean age = 19.8 years, SD = 0.88, 153 women). Data were collected using the Vulnerability Scale for University Athletes and General Coping Questionnaire and analyzed by conducting a multivariate analysis of variance. The results showed significant relationships between vulnerability and coping strategies (r = .11−.39). Vulnerability was most strongly related to the emotional support seeking aspect of emotion-oriented coping (r = .39). There was no significant difference in cognitive reinterpretation (r = .07). Vulnerability had a stronger relationship with emotion-oriented than problem-oriented coping, and high (vs. low) vulnerability athletes had significantly higher emotion-oriented-coping scores. These results suggest that vulnerable athletes need to be provided with appropriate emotional support to cope with stressful situations, as they rely heavily on a stress management strategy focusing on emotion regulation.
Emotional Intelligence Amongst Millennials: Male vs. Female Leaders in the IT and ITES Sectors

The Millennial generation constitutes the majority of the present day workforce. As per the birth year and age group they presently belong to, many Millennial are already in managerial roles and many shall occupy key leadership roles in their respective organizations. Leadership being an emotion laden process requires the millennials to be adaptable so that they can motivate their team members or followers, understand their requirements and reduce their stress levels. Emotional Intelligence plays a crucial role in addressing these workplace issues and correlates positively with leadership. The study is focused on Indian Information Technology (IT) and ITES organizations as it is one of the biggest employers and contributes majorly towards the country’s economy. The present study tries to compare the level of emotional intelligence of millennials across gender, management levels, qualifications and years of experiences. Practical implications related to gender diversity and inclusion of women in leadership roles has also been discussed.
Evaluation of the Thermal Stability of a Vaccine Prototype Based on Virus-like Particle Formulated HIV-1 Envelope

The long-term storage stability of vaccines has a major impact on the roll-out and success of global immunization programs. For the Human Immunodeficiency Virus type 1 (HIV-1) virus-like particle (VLP) vaccine prototype evaluated here, nanoparticle tracking analysis (NTA), and enzyme-linked immunoabsorbent assay (ELISA) results demonstrated a remarkable structural stability. VLPs maintained their integrity and the recognition of relevant B-cell epitopes for three months at 4 and −20 °C. Interestingly, most particles remained intact and preserved the recognition of relevant epitopes even after a week of storage at room temperature.
Evaluation of the Predictive Value of Urine Leukocyte Esterase Test in Chlamydia trachomatis and Neisseria gonorrhoeae Infection Among Males Attending HIV/STI Clinics in Guangdong Province, China

Leukocyte esterase test (LET) detection is a simple and inexpensive test performed by urinalysis. This study investigated the predictive value of LET for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infection among men attending HIV and sexually transmitted infection (HIV/STI) clinics in Guangdong Province, China. A total of 5,509 urine samples were collected from HIV and sexually transmitted infection clinics in Guangdong Province between 2017 and 2019. Specimens from 5,464 males were tested by both LET and nucleic acid amplification test (NAAT). Of 5,464 males, 497 (9.1%) tested positive for CT or NG by NAAT, with respective prevalence rates of 6.4% (95% confidence interval [95% CI]: 5.8–7.1%) and 3.8% (95% CI: 3.3–4.3%), including 1.2% (95% CI: 0.9–1.4%) co-infected. Compared to the HIV-negative individuals, individuals living with HIV tend to have a higher prevalence of CT, NG and co-infection with CT and NG. The LET sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for CT were 46.4% (95% CI: 41.2–51.7%), 92.0% (95% CI: 91.2–92.7%), 28.4% (95% CI: 24.8–32.1%), and 96.1% (95% CI: 95.6–96.7%), respectively. The LET sensitivity, specificity, PPV, and NPV for NG were 68.4% (95% CI: 62.1–74.7%), 91.8% (95% CI: 91.1–92.6%), 25.0% (95% CI: 21.4–28.5%), and 98.7% (95% CI: 98.3–99%), respectively. Compared to the HIV-negative individuals, higher sensitivity and specificity were observed for HIV-positive individuals, but there was no statistical difference. The incremental cost-effectiveness ratio (ICER) using economic costs per additional person CT positive and NG positive was –$238.74 and –$145.60 compared with LET positive, respectively. LET is a cost-effective test and will be valuable for predicting CT and NG infection, which is highly prevalent in low- and middle-income countries.
Effects of Corruption Control on the Number of Undernourished People in Developing Countries

Developing countries will be home to 85% of the world’s population by 2030. Hence, it is important to ensure food security for them. This effort is not easy, as the number of undernourished people (NUP) in the world has increased. We investigated the impact of food and non-production factors on the NUP in developing countries. This study employed secondary data from 57 developing countries between 2002 and 2018. These countries come from three regions, namely Africa, Asia, and Latin America and the Caribbean. One-step and two-step generalized method of moments (sys-GMM) models were used to analyze the data. The findings showed that the food production index, cereal import dependency ratio, economic globalization index, and human capital index had different effects on the NUP in each region. The excellent news is that corruption control can help developing countries minimize their NUP. Based on the findings, we propose efforts to improve physical and economical food access and control corruption, and developing country governments and the international community must demonstrate a strong commitment to reducing the prevalence of undernourishment.
Diversity-Oriented Synthesis Catalyzed by Diethylaminosulfur-Trifluoride—Preparation of New Antitumor Ecdysteroid Derivatives

Fluorine represents a privileged building block in pharmaceutical chemistry. Diethylaminosulfur-trifluoride (DAST) is a reagent commonly used for replacement of alcoholic hydroxyl groups with fluorine and is also known to catalyze water elimination and cyclic Beckmann-rearrangement type reactions. In this work we aimed to use DAST for diversity-oriented semisynthetic transformation of natural products bearing multiple hydroxyl groups to prepare new bioactive compounds. Four ecdysteroids, including a new constituent of Cyanotis arachnoidea, were selected as starting materials for DAST-catalyzed transformations. The newly prepared compounds represented combinations of various structural changes DAST was known to catalyze, and a unique cyclopropane ring closure that was found for the first time. Several compounds demonstrated in vitro antitumor properties. A new 17-N-acetylecdysteroid (13) exerted potent antiproliferative activity and no cytotoxicity on drug susceptible and multi-drug resistant mouse T-cell lymphoma cells. Further, compound 13 acted in significant synergism with doxorubicin without detectable direct ABCB1 inhibition. Our results demonstrate that DAST is a versatile tool for diversity-oriented synthesis to expand chemical space towards new bioactive compounds.
Effect of Glycerol, Calcium and Transglutaminase Post-Treatment on the Properties of Regenerated Fibers from Rennet-Treated Casein Micelles

Regenerated fibers can be prepared from a cooled solution of renneted casein micelles in a wet spinning process. For better handling and stability of the fiber, plasticizers, network modifiers or cross-linkers are used in the production process. For that reason, fibers with different glycerol and calcium content are prepared in this study and subsequently treated with the enzyme transglutaminase before being characterized after air drying. In addition to the swelling behavior in NaOH, H2O, simulated milk ultrafiltrate buffer as well as HCl, the mechanical properties of the fibers are investigated, taking into account their microscopic fine structure. Transglutaminase-treated fibers show sigmoidal absorption curves for all solvents and reach higher equilibrium swelling percentages than untreated fibers. When the calcium content in the coagulation bath is increased from 50 mM to 100 mM, more stabilizing calcium bridges lead to a denser fiber structure that swells more slowly in all solvents considered. With increasing glycerol content, the flexibility of the fibers increases, as indicated by the decrease in elastic moduli, and a fine structure in the sub-µm range becomes visible. The fibers also demonstrate lower elastic moduli when post-treated with transglutaminase. Besides the higher casein content due to the transglutaminase treatment, this could also contribute to the higher equilibrium swelling percentages compared to the untreated fibers.
A review of saproxylic beetle intra- and interspecific genetics: current state of the knowledge and perspectives

Abstract Forests are important habitats that harbor high biodiversity and have great economic value to humans. These ecosystems depend on saproxylic organisms, which play a crucial role in the decomposition of the main substrate of forests – wood. The highly important group of saproxylic organisms are beetles, with numerous species that are either of conservation concern (rare and threatened) or have economic value (“pests”). This review summarizes what is known about intra- and interspecific genetic information on species or populations of saproxylic beetles. A search of the literature yielded 110 articles published in 57 journals. The majority of molecular research has focused on “pests” (82 articles on 59 species), with fewer studies of the “non-pest” beetles (28 articles on 40 species, including 10 relicts of primeval forests). Most studies concerned Palearctic and Nearctic taxa, with only a few articles on beetles from (sub)tropical forests. Members of 11 families, 38 genera and 99 species were the subject of molecular studies. The majority of studies (71%) were conducted on beetles associated with conifers. Genetic metrics were found to have different overall values in “pests” and the “non-pest” species. Analyzes resulted in identification of units or either conservation (15 cases) or taxonomic (33) value, the discovery of hybridization (4), invasion routes, and common phylogeographic patterns with the localization of glacial refugial areas. This article is the first comprehensive summary of what is known about the history, present-day diversity, and relationships of highly important group of saproxylic beetles based on molecular studies. “Pests” found to be much wider studied than threatened taxa. A list of species that should be the subject of future molecular studies was provided along with a rationale. Finally, methodological and objective deficiencies in previous studies were characterized.
Should I Go to Graduate School? The Role of Preference for Children and Human Capital Accumulation

We propose a selection mechanism with preference heterogeneity to study the impact of children on women’s decision to attend graduate school. We show that the net return to education decreases with women’s preferences for children and that only women with preferences below a certain threshold find it optimal to go to graduate school. We embed the selection mechanism into a dynamic life-cycle model of schooling, fertility, and labor force participation decisions and find that changes in returns to experience are quantitatively relevant to account for the observed changes in behavior of women born in 1945 or 1965.
The Separation of the Unpaved Roads and Prioritization of Paving These Roads Using UAV Images

Prioritization of pathways to perform asphalt pavement operations has always been one of the most important concerns for municipalities, for which, currently there is no specific planning and pattern. In the present study, using (Unmanned Aerial Vehicle) UAV images, a land cover map of the case study was prepared. For this purpose, the accuracy of various object-based classification methods including the Bayes method, the Support Vector Machine (SVM), the K nearest neighbor (KNN), the Decision tree (DT), and the Random tree (RT) was investigated. Findings of the study showed that by increasing heterogeneity in the composition of the studied phenomenon in the image, different classification algorithms offer results different from each other. The obtained results of the accuracy evaluation of classification methods indicate that the SVM method with 80% kappa coefficient and 89% overall accuracy had the best performance compared to other methods. As a result, built-up land covers, bare land, vegetation cover, and paved roads were separated using this method. Then, the exact boundary of pathways was prepared using Google Earth images, and then, using the land-use map prepared from the case study, the roads were divided into two categories: paved and unpaved. To determine the prioritization of unpaved roads for applying asphalt, the proportion of built-up lands (BUL) to bare (non-built-up) lands (BL) was used in each path. Based on the obtained results, 1% of the roads in the case study was placed on a very high level of asphalt, and then 9%, 3%, 49%, 38%, were placed on a high priority to low priority, respectively.
Factors Influencing the Molecular Compositions and Distributions of Atmospheric Nitrogen‐Containing Compounds

Atmospheric nitrogen‐containing organic compounds (NOCs) are critical components of global nitrogen deposition and light‐absorption species. The sources and compositions of NOCs are complex and remain largely unknown. Here, NOCs in 55 ambient aerosol samples collected in Guangzhou, South China, were analyzed via ultrahigh‐resolution Fourier transform ion cyclotron resonance mass spectrometry in negative‐ion and positive‐ion electrospray ionization (ESI) modes. The molecular compositions of NOCs measured via ESI– and ESI+ exhibited considerable differences. NOCs detected in the negative mode were mainly composed of highly oxygenated organic nitrates (O/N = 6), whereas NOCs detected in the positive mode were mainly composed of reduced nitrogen‐containing compounds (e.g., amides and amino acids). CHN compounds potentially corresponding to amines and alkaloids showed low abundance in the detection modes. Non‐metric multidimensional scaling and individual compound correlation analyses showed that the molecular compositions of NOCs were mainly affected by anthropogenic activities and meteorological parameters. For example, anthropogenic activities such as biomass burning and secondary nitrogen‐chemistry processes led to the accumulation of aromatic and highly oxygenated NOCs during winter. During summer, higher OH radical concentrations and temperatures will result in more prevalent or persistent reduced aliphatic NOCs, particularly lipid‐like amines. Some variables (e.g., relative humidity) have distinct effects on the variation of different types of NOCs. More research is needed to reveal the influencing mechanisms. This study clarifies the molecular compositions of NOCs and the mechanisms by which various factors influence the molecular variations. The findings can guide the assessment of NOCs evolution and deposition.
With or Without You: Beneficial and Detrimental Associations Between Companion Dogs and Human Psychological Adjustment During a COVID-19 Lockdown Phase

ABSTRACT It has been proposed that companion animals, and dogs in particular, may be helpful during the COVID-19 pandemic, notably for psychological adjustment. The present study addressed this idea in a sample of 509 Portuguese individuals exposed to a national lockdown. Linear regression analyses were performed to test for associations between having lived with a dog during that phase and experienced levels of anxiety and depression while considering potential moderating effects. Sub-analyses on 345 dog owners were performed to explore how different dimensions of the dog–owner relationship are associated with owners’ anxiety and depression levels. A complex association was found between having lived with a dog during the lockdown, living area, and anxiety. For participants in rural and semi-urban areas, living with a dog was associated with lower anxiety; for participants in urban areas, living with a dog was associated with higher anxiety. Among dog owners, dog walking was marginally associated with lower depression, while perceived costs derived from caring for a dog and emotional closeness were associated with higher anxiety and depression. The results highlight the importance of going beyond mere ownership when studying the effects of companion dogs on human health and wellbeing. Also, they call for attention to dogs as a source of both concern and potential for health planners when defining strategies to manage mental health.
Information systems implementation and user performance in the county government of Machakos, Kenya

This paper aims to examine the impact of IS Implementation on User Performance in the county Government of Machakos, Kenya. This paper made use of descriptive designs as well explanatory study designs. In addition, the research made use of primary data that was gathered using open and closed-ended questions in a research questionnaire. Data collected were analyzed using the SPSS software. It was established that IS implementation had a positive effect on user performance which in return impacts the organizational performance. The study revealed that IS's ability is to provide high-quality information, which reduces errors and resolves performance problems when they occur. The study concluded that IS is a major factor influencing user performance in Machakos County Government, Kenya and that there was a positive relationship between independent variables and dependent variables. Therefore, this study recommends that County Governments should invest heavily in IS without fear as this will improve their employee’s performance and in return improve the performance of the County Governments in terms of service delivery to the citizens.