Miracle-dz/UniFAR-FTUs huggingface dataset:
Trailing-edge fringes enable robust aerodynamic force production and noise suppression in an owl wing model

As one of the unique owl-wing morphologies, trailing-edge (TE) fringes are believed to play a critical role in the silent flight of owls and have been widely investigated using idealized single/tandem airfoils. However, the effect of TE fringes and associated mechanisms on the aeroacoustics of owl wings, which feature curved leading edges, wavy TEs, and several feather slots at the wingtips, have not yet been addressed. In this study, we constructed two 3D owl wing models, one with and one without TE fringes, based on the geometric characteristics of a real owl wing. Large-eddy simulations and the Ffowcs Williams‒Hawkings analogy were combined to resolve the aeroacoustic characteristics of the wing models. Comparisons of the computed aerodynamic forces and far-field acoustic pressure levels demonstrate that the fringes on owl wings can robustly suppress aerodynamic noise while sustaining aerodynamic performance comparable to that of a clean wing. By visualizing the near-field flow dynamics in terms of flow and vortex structures as well as flow fluctuations, the mechanisms of TE fringes in owl wing models are revealed. First, the TE fringes on owl wings are reconfirmed to robustly suppress flow fluctuations near the TE by breaking up large TE vortices. Second, the fringes are observed to effectively suppress the shedding of wingtip vortices by mitigating the flow interaction between feathers (feather-slot interaction). These complementary mechanisms synergize to enhance the robustness and effectiveness of the TE fringe effects in owl wing models, in terms of aerodynamic force production and noise suppression. This study thus deepens our understanding of the role of TE fringes in real owl flight gliding and points to the validity and feasibility of employing owl-inspired TE fringes in practical applications of low-noise fluid machinery.
Estimating the environmental impact of endoscopic activity at a tertiary center: a pilot study

Introduction The growing number of endoscopic procedures, frequently requiring single-use disposable instruments, is responsible for the production of a large amount of waste. To this date, the reality of waste production at large European Gastroenterology centers is unknown. This study aimed to estimate the amount of waste due to endoscopic practice at a tertiary center in Portugal. Methods We performed a prospective study to calculate the mass (in kg) of residues generated during a period of 5 working days of endoscopic practice. We included residues produced at endoscopy suites, pre and postprocedure areas and during endoscope reprocessing. Residues were categorized as non-dangerous (groups I/II), of biologic risk (group III) and specific hazardous hospital residues (group IV). The production of residues separated for recycling/valorization (paper/card and plastic) was also quantified. The volume of water used for reprocessing an endoscope was also assessed. Results During the analyzed period, 241 endoscopic procedures were performed. A total of 443.2 kg of waste (22.6 kg from groups I/II, 266.9 kg from group III and 3.9 kg from group IV) were produced, most from group III (75%). For each endoscopic procedure, 1.8 kg of waste was generated. Of the total waste mass, 17.8% was separated for recycling/valorization. A volume of 55L of water was required for reprocessing one endoscope. Conclusion Each endoscopic procedure generated a significant amount of waste and water consumption during reprocessing. These real-life analyses are a pivotal step before implementing effective measures to improve resource utilization and more sustainable practices.
Seasonal patterns of sickness absence due to diagnosed mental disorders: a nationwide 12-year register linkage study

Abstract Aims Although seasonality has been documented for mental disorders, it is unknown whether similar patterns can be observed in employee sickness absence from work due to a wide range of mental disorders with different severity level, and to what extent the rate of change in light exposure plays a role. To address these limitations, we used daily based sickness absence records to examine seasonal patterns in employee sickness absence due to mental disorders. Methods We used nationwide diagnosis-specific psychiatric sickness absence claims data from 2006 to 2017 for adult individuals aged 16–67 (n = 636,543 sickness absence episodes) in Finland, a high-latitude country with a profound variation in daylength. The smoothed time-series of the ratio of observed and expected (O/E) daily counts of episodes were estimated, adjusted for variation in all-cause sickness absence rates during the year. Results Unipolar depressive disorders peaked in October–November and dipped in July, with similar associations in all forms of depression. Also, anxiety and non-organic sleep disorders peaked in October–November. Anxiety disorders dipped in January–February and in July–August, while non-organic sleep disorders dipped in April–August. Manic episodes reached a peak from March to July and dipped in September–November and in January–February. Seasonality was not dependent on the severity of the depressive disorder. Conclusions These results suggest a seasonal variation in sickness absence due to common mental disorders and bipolar disorder, with high peaks in depressive, anxiety and sleep disorders towards the end of the year and a peak in manic episodes starting in spring. Rapid changes in light exposure may contribute to sickness absence due to bipolar disorder. The findings can help clinicians and workplaces prepare for seasonal variations in healthcare needs.
Stargardt macular dystrophy and therapeutic approaches

Stargardt macular dystrophy (Stargardt disease; STGD1; OMIM 248200) is the most prevalent inherited macular dystrophy. STGD1 is an autosomal recessive disorder caused by multiple pathogenic sequence variants in the large ABCA4 gene (OMIM 601691). Major advances in understanding both the clinical and molecular features, as well as the underlying pathophysiology, have culminated in many completed, ongoing and planned human clinical trials of novel therapies. The aims of this concise review are to describe (1) the detailed phenotypic and genotypic characteristics of the disease, multimodal imaging findings, natural history of the disease, and pathogenesis, (2) the multiple avenues of research and therapeutic intervention, including pharmacological, cellular therapies and diverse types of genetic therapies that have either been investigated or are under investigation and (3) the exciting novel therapeutic approaches on the translational horizon that aim to treat STGD1 by replacing the entire 6.8 kb ABCA4 open reading frame.
Concurrence of inflammatory bowel disease with multiple sclerosis or Hodgkin lymphoma

Background and aims Epidemiologic evidence suggests that Hodgkin lymphoma (HL) and multiple sclerosis (MS) share a common set of risk factors with Crohn’s disease (CD) and ulcerative colitis (UC). It was hypothesized that such shared risk factors would lead to clustering of the 4 diagnoses in the same patients. Methods All patients with HL, MS, CD, or UC were identified in the veterans population from 2016–2020 and the Medicare population from 1986 to 1989. In a case-control study, the observed concurrences amongst these 4 diagnoses were compared with their expected frequencies in the overall veterans or Medicare population during the same time period by calculating odds ratios (OR) with their 95% confidence intervals (CI). Results The study included 6 million veterans and 35 million Medicare patients. In the veterans population, inflammatory bowel disease (IBD) was significantly associated with a concurrent diagnosis of HL (OR: 1.40, 95% CI: 1.15–1.71) and MS (1.34, 1.19–1.50). In the Medicare population, IBD was also significantly associated with HL (1.84, 1.07–3.17) and MS (2.31, 1.59–3.35). Similar trends were observed in CD or UC when analyzed separately in both datasets. In the veterans population, adjustment for the potentially confounding influence of ethnicity, sex, and age left all OR values largely unaffected and statistically significant. Conclusion The concurrence of IBD with HL or MS could reflect on a common pathway in the etiology or pathogenesis of these 4 diseases.
Impact of the interaction between the quasi‐2 day wave and tides on the ionosphere and thermosphere

The quasi‐2 day wave (QTDW) can effectively interact with atmospheric tides in the mesosphere and lower thermosphere. We study the effect of this QTDW‐tidal interaction on the ionosphere and thermosphere using the thermosphere‐ionosphere‐mesosphere electrodynamics general circulation model. This interaction reduces the amplitude of the migrating diurnal tide in the lower thermosphere by ~10 m/s in neutral winds and also generates sum and difference secondary waves in the lower thermosphere and E region ionosphere. As a result of the changed migrating diurnal tide and sum/difference secondary wave, vertical ion drift varies with local time at different longitudes by ~5 m/s. In addition, the changed migrating diurnal tide also modulates the thermospheric composition (O/N2). During a QTDW event, the ionosphere F2 region peak electron density (NmF2) is reduced due to the mixing effect of the QTDW dissipation; NmF2 also shows changes in local time variation due to the QTDW‐tidal interaction. The sum and difference secondary waves can cause additional oscillations in vertical ion drift and ionospheric electron densities. The QTDW‐tidal interaction is another mechanism by which the QTDW impacts the ionosphere and thermosphere, along with other mechanisms: QTDW modulation of the E region wind dynamo with a period of quasi‐2 days and QTDW dissipation induced mixing in the thermosphere.
Structural evolution of the High Himalayan Gneiss sequence, Langtang Valley, Nepal

Abstract Within the High Himalayan Crystalline Sequence (HHCS) of the Langtang Valley of central Nepal, four different lithotectonic units have been identified in the field. The structurally lowest unit, immediately in the hanging wall of the Main Central Thrust (MCT) records an inverted metamorphic sequence with kyanite-grade rocks overlain by rocks containing sillimanite. Pressure-temperature-deformation histories of the different units since Himalayan collision 50 Ma ago, in combination with geochemical data, indicates that the ‘apparent’ metamorphic inversion is a result of the tectonic juxtaposition along high-temperature shear zones that accommodated top-to-southwest thrusting. It is also argued that the contacts between different sillimanite-grade units are also tectonic in origin and that the HHCS in the Langtang Valley does not represent a structurally undisturbed section through the crust. Internally the different units contain minor extensional and thrust faults which at the same structural level developed at similar metamorphic conditions. Deformation along the high temperature shear zones of the HHCS are coeval with the earliest of these minor fault zones at the lowest structural levels. With time, this deformation migrated upwards and minor faulting within the upper levels of the HHCS took place at chlorite-grade. These minor fault zones are interpreted to be the result of dynamic spreading of the developing orogenic wedge associated with changes in the geometry of this wedge due to underplating and accretion of material to the base of the metamorphic pile.
Metagenomics harvested genus-specific single-stranded DNA-annealing proteins improve and expand recombineering in Pseudomonas species

Abstract The widespread Pseudomonas genus comprises a collection of related species with remarkable abilities to degrade plastics and polluted wastes and to produce a broad set of valuable compounds, ranging from bulk chemicals to pharmaceuticals. Pseudomonas possess characteristics of tolerance and stress resistance making them valuable hosts for industrial and environmental biotechnology. However, efficient and high-throughput genetic engineering tools have limited metabolic engineering efforts and applications. To improve their genome editing capabilities, we first employed a computational biology workflow to generate a genus-specific library of potential single-stranded DNA-annealing proteins (SSAPs). Assessment of the library was performed in different Pseudomonas using a high-throughput pooled recombinase screen followed by Oxford Nanopore NGS analysis. Among different active variants with variable levels of allelic replacement frequency (ARF), efficient SSAPs were found and characterized for mediating recombineering in the four tested species. New variants yielded higher ARFs than existing ones in Pseudomonas putida and Pseudomonas aeruginosa, and expanded the field of recombineering in Pseudomonas taiwanensisand Pseudomonas fluorescens. These findings will enhance the mutagenesis capabilities of these members of the Pseudomonas genus, increasing the possibilities for biotransformation and enhancing their potential for synthetic biology applications.
Impact of pulsed field ablation on intraluminal esophageal temperature

Atrio‐esophageal fistula after esophageal thermal injury (ETI) is one of the most devastating complications of available energy sources for atrial fibrillation (AF) ablation. Pulsed field ablation (PFA) uses electroporation as a new energy source for catheter ablation with promising periprocedural safety advantages over existing methods due to its unique myocardial tissue sensitivity. In preclinical animal studies, a dose‐dependent esophageal temperature rise has been reported. In the TESO‐PFA registry intraluminal esophageal temperature (TESO) changes in a clinical setting are evaluated.
Analysis of Surgical Complications in Mandibular Fractures in the Center of Italy: A Retrospective Study

Mandibular fractures are the second most common maxillofacial fractures. The prevalent treatment for this kind of fractures is either a surgical procedure such as maxillomandibular fixation or open reduction and internal fixation or a combination of both. Sometimes the patients might develop locoregional postoperative complications. The most frequently reported complications are trigeminal V3 injury and surgical site infection. A 4-year retrospective study was performed at the Department of Oral and Maxillofacial Surgery, Policlinico Umberto I of Rome, Italy, and the postoperative complications after mandibular fracture surgical treatment were collected. The authors studied the characteristics of each complication and the correlation with the type of mandibular fracture and the surgical treatment chosen.
Phenotyping the Responses to Systemic Corticosteroids in the Management of Asthma Attacks (PRISMA): protocol for an observational and translational pilot study

Introduction Asthma and its associated exacerbation are heterogeneous. Although severe asthma attacks are systematically prescribed corticosteroids and often antibiotics, little is known about the variability of response to these therapies. Blood eosinophils and fractional exhaled nitric oxide (FeNO) are type 2 inflammation biomarkers that have established mechanistic, prognostic and theragnostic values in chronic asthma, but their utility in acute asthma is unclear. We speculate that the clinical and biological response to those treatments varies according to inflammometry and microbiological test results. Methods and analysis An observational longitudinal pilot study with multimodal clinical and translational assessments will be performed on 50 physician-diagnosed ≥12-year-old asthmatics presenting with an asthma attack and 12 healthy controls, including blood eosinophil count (venous and point-of-care (POC) capillary blood), FeNO and testing for airway infection (sputum cultures and POC nasopharyngeal swabs). People with asthma will be assessed on day 0 and after a 7-day corticosteroid course, with home monitoring performed in between. The primary analysis will be the change in the forced expiratory volume in 1 s according to type 2 inflammatory status (blood eosinophils ≥0.15×109/L and/or FeNO ≥25 ppb) after treatment. Key secondary analyses will compare changes in symptom scores and the proportion of patients achieving a minimal clinically important difference. Exploratory analyses will assess the relationship between clinical, lung function, inflammatory and microbiome parameters; satisfaction plus reliability indices of POC tests; and sex–gender variability in treatment response. Ultimately, this pilot study will serve to plan a larger trial comparing the clinical and biological response to systemic corticosteroids according to inflammatory biomarkers, offering valuable guidance for more personalised therapeutic strategies in asthma attacks. Ethics and dissemination The protocol has been approved by the Research Ethics Committee of the CIUSSS de l'Estrie–CHUS, Sherbrooke, Quebec, Canada (#2023-4687). Results will be communicated in an international meeting and submitted to a peer-reviewed journal. Trial registration number ClinicalTrials.gov Registry (NCT05870215).
Abnormal functional connectivity strength in age-related macular degeneration patients: a fMRI study

Objective Age-related macular degeneration (AMD) is a serious blinding eye disease. Previous neuroimaging studies reported that AMD were accompanied by abnormalities of the brain. However, whether AMD patients were associated with functional connectivity strength (FCS) or not remains unknown. In our study, the purpose of the study was to assess FCS changes in AMD patients. Methods In our study, 20 AMD patients and 20 healthy controls (HCs), matched closely by sex, age, and educational level were underwent MRI scanning. FCS method and seed-based functional connectivity (FC) method were applied to investigate the functional network changes between two groups. Moreover, support vector machine (SVM) method was applied to assess the FCS maps as a feature to classification of AMD diseases. Results Our study reported that AMD patients showed decreased FCS values in the bilateral calcarine, left supplementary motor area, left superior parietal lobule and left paracentral lobule (ParaL) relative to the HC group. Meanwhile, our study found that the AMD patients showed abnormal FC within visual network, sensorimotor network and default mode network. Moreover, the SVM method showed that FCS maps as machine learning features shows good classification efficiency (area under curve = 0.82) in the study. Conclusion Our study demonstrated that AMD patients showed abnormal FCS with the visual network, sensorimotor network and default mode network, which might reflect the impaired vision, cognition and motor function in AMD patients. In addition, FCS indicator can be used as an effective biological marker to assist the clinical diagnosis of AMD.
Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

The efficacy of 40 bacterial isolates obtained from hot spring water samples to produce cellulase enzymes was investigated. As a result, the strain Bacillus subtilis F3, which was identified using traditional and molecular methods, was selected as the most potent for cellulase production. Optimization was carried out using one-factor-at-a-time (OFAT) and BOX-Behnken Design to detect the best conditions for the highest cellulase activity. This was accomplished after an incubation period of 24 h at 45°C and pH 8, with an inoculum size of 1% (v/v), 5 g/l of peptone as nitrogen source, and 7.5 g/l of CMC. Moreover, the best concentration of ammonium sulfate for cellulase enzyme precipitation was 60% followed by purification using a dialysis bag and Sephadex G-100 column chromatography to collect the purified enzyme. The purified cellulase enzyme was characterized by 5.39-fold enrichment, with a specific activity of 54.20 U/mg and a molecular weight of 439 kDa. There were 15 amino acids involved in the purified cellulase, with high concentrations of 160 and 100 mg/l for glycine and proline respectively. The highest stability and activity of the purified cellulase was attained at pH 7 and 50°C in the presence of 150 ppm of CaCl2, NaCl, and ZnO metal ions. Finally, the biopolishing activity of the cellulase enzyme, as indicated by weight loss percentages of the cotton fabric, was dependent on concentration and treatment time. Overall, the thermotolerant B. subtilis F3 strain has the potential to provide highly stable and highly active cellulase enzyme for use in biopolishing of cotton fabrics.
Danish Prostate Cancer Consortium Study 1 (DPCC-1) protocol: Multicentre prospective validation of the urine-based three-microRNA biomarker model uCaP

Introduction The primary objective of the Danish Prostate Cancer Consortium Study 1 (DPCC-1) is to provide validation for a novel urine-based microRNA biomarker, called uCaP, for a diagnosis of prostate cancer. Methods and analysis Eligible participants are biopsy naïve men aged ≥18 years with prostate-specific antigen (PSA) levels ≥3 ng/mL, who are referred to prostate MRI due to suspicion of PC at one of the following three major urology/uroradiology centers: Aarhus University Hospital, Herlev & Gentofte University Hospital, or Odense University Hospital, where MRI and targeted biopsy are implemented in clinical use. Exclusion criteria include previous diagnosis of urogenital cancer, contraindication to MRI, gender reassignment treatment or PSA level >20 ng/mL. The participants will be asked to donate a urine sample in connection with their MRI. The study is observational, uses a diagnostic accuracy testing setup and will integrate into the current diagnostic pathway. We will measure the levels of the three microRNAs in the uCaP model (miR-222–3 p, miR-24–3 p and miR-30c-5p) in extracellular vesicle-enriched cell-free urine samples, to assess if uCaP can improve specificity and retain sensitivity for International Society of Urological Pathology Grade Group ≥2 PC, when used as a reflex test to PSA ≥3 ng/mL. We hypothesise that uCaP can improve selection for prostate MRI and reduce the number of unnecessary scans and biopsies. Ethics and dissemination This study is approved by the Central Denmark Region Committee on Health Research Ethics (reference number: 1-10-72-85-22). All participants will provide written informed consent. Study results will be published in peer-reviewed journals and presented in scientific meetings. Trial registration number NCT05767307 at clinicaltrials.gov.