Miracle-dz/UniFAR-FTUs huggingface dataset:
Circadian Pattern of Acute Myocardial Infarction and Atrial Fibrillation in a Mediterranean Country: A study in Diabetic Patients

Background and objectives: The circadian pattern seems to play a crucial role in cardiovascular events and arrhythmias. Diabetes mellitus is a complex metabolic disorder associated with autonomic nervous system alterations and increased risk of microvascular and macrovascular disease. We sought to determine whether acute myocardial infarction (AMI) and atrial fibrillation (AF) follow a circadian pattern in diabetic patients in a Mediterranean country. Materials and Methods: This retrospective study included 178 diabetic patients (mean age: 67.7) with AMI or AF who were admitted to the coronary care unit. The circadian pattern of AMI and AF was identified in the 24-h period (divided in 3-h and 1-h intervals). Patients were also divided in 3 groups according to age; 40–65 years, 66–79 years and patients older than 80 years. A chi-square goodness-of-fit test was used for the statistical analysis. Results: AMI seems to occur more often in the midnight hours (21:00–23:59) (p < 0.001). Regarding age distribution, patients between 40 and 65 years were more likely to experience an AMI compared to other age groups (p < 0.001). Autonomic alterations, working habits, and social reasons might contribute to this phenomenon. AF in diabetic patients occurs more frequently at noon (12:00–14:59) (p = 0.019). Conclusions: Diabetic patients with AMI and AF seem to follow a specific circadian pattern in a Mediterranean country, with AMI occurring most often at midnight hours and AF mostly at noon. Autonomic dysfunction, glycemic fluctuations, intense anti-diabetic treatment before lunch, and patterns of insulin secretion and resistance may explain this pattern. More studies are needed to elucidate the circadian pattern of AMI and AF in diabetic patients to contribute to the development of new therapeutic approaches in this setting.
Dietary Salt-Related Determinants of Hypertension in Rural Northern Thailand

Hypertension and its connection to high salt consumption have been observed in the Thai population. This study mainly contributed to the literature to examine the dietary-salt-related determinants associated with the risk of hypertension in rural northern Thailand, which exhibited the highest prevalence of hypertension. A total of 376 adults residing in San Pa Tong District, Chiang Mai province, were face-to-face interviewed using a structured questionnaire assessing dietary-salt-related knowledge, attitudes, consumption, sources, and habits. The subject’s blood pressure (BP) was measured twice before and after the interview. Hypertension was defined as a systolic BP ≥ 130 mmHg or a diastolic BP ≥ 80 mmHg. The dietary-salt-related knowledge, attitude, and habits toward salt reduction were positively correlated; however, knowledge and attitudes were not significantly correlated with consumption. Multivariate logistic regression results indicated subjects who frequently bought ready-to-eat food, ate out, or used bouillon cube/monosodium glutamate (MSG) during food preparation were likely to have hypertension (OR = 2.24, 95% CI: 1.36–3.69, p = 0.002). MSG was heavily consumed and used as a flavor enhancer in northern Thai cuisine; however, a few subjects realized it contains sodium due to no salty taste. The deficiency of specific dietary-salt-related knowledge illustrated the need for tailored educational intervention strategies.
Effect of Benzoylphenyl Ureas on Survival and Reproduction of the Lace Bug, Leptopharsa gibbicarina

Simple Summary Pestalotiopsis fungal complex is a disease that causes damages in oil palm (Elaeis guineensis), and the lace bug, Leptopharsa gibbicarina is the main insect pest that spread this disease. Application of neurotoxic insecticides has been a common method used to control L. gibbicarina for decades in Colombia and Venezuela. The effects of four benzoylphenyl ureas (BPUs) (lufenuron, novaluron, teflubenzuron, and triflumuron) were assessed against L. gibbicarina for toxicity, survival, and reproduction. Overall, the results show that novaluron, teflubenzuron, and triflumuron cause high mortality and reduce survival time, fecundity, and fertility. Thus, BPUs exhibit detrimental effects on L. gibbicarina and can be used as alternatives to other chemical insecticides. Abstract The lace bug, Leptopharsa gibbicarina is a vector of Pestalotiopsis fungal complex in oil palm crops in the Americas. The effects of four benzoylphenyl ureas (BPUs) (lufenuron, novaluron, teflubenzuron, and triflumuron) were evaluated against L. gibbicarina for toxicity, survival, reproduction, and mortality in semi-field conditions. Concentration-mortality bioassays demonstrated that novaluron (LC50 = 0.33 ppm), teflubenzuron (LC50 = 0.24 ppm), lufenuron (LC50 = 0.17 ppm), and triflumuron (LC50 = 0.42 ppm) are toxic to L. gibbicarina nymphs. The survival rate was 99% in control nymphs, decreasing to 50% in nymphs exposed to LC50 of triflumuron, 47% in nymphs treated with lufenuron, 43% in nymphs treated with teflubenzuron, and 43% in those treated with novaluron. Sublethal concentrations of BPUs showed detrimental effects on the adult emergence, longevity, fecundity, and fertility of this insect. The mortality of nymphs caused by these insecticides was similar in both laboratory and semi-field conditions. Our results suggest that novaluron, teflubenzuron, and triflumuron are highly effective against L. gibbicarina, and therefore, have potential applications for this oil palm pest.
Transcriptomic analysis of the honey bee (Apis mellifera) queen spermathecae reveals genes that may be involved in sperm storage after mating

Honey bee (Apis mellifera) queens have a remarkable organ, the spermatheca, which successfully stores sperm for years after a virgin queen mates. This study uniquely characterized and quantified the transcriptomes of the spermathecae from mated and virgin honey bee queens via RNA sequencing to identify differences in mRNA levels based on a queen’s mating status. The transcriptome of drone semen was analyzed for comparison. Samples from three individual bees were independently analyzed for mated queen spermathecae and virgin queen spermathecae, and three pools of semen from ten drones each were collected from three separate colonies. In total, the expression of 11,233 genes was identified in mated queen spermathecae, 10,521 in virgin queen spermathecae, and 10,407 in drone semen. Using a cutoff log2 fold-change value of 2.0, we identified 212 differentially expressed genes between mated and virgin spermathecal queen tissues: 129 (1.4% of total) were up-regulated and 83 (0.9% of total) were down-regulated in mated queen spermathecae. Three genes in mated queen spermathecae, three genes in virgin queen spermathecae and four genes in drone semen that were more highly expressed in those tissues from the RNA sequencing data were further validated by real time quantitative PCR. Among others, expression of Kielin/chordin-like and Trehalase mRNAs was highest in the spermathecae of mated queens compared to virgin queen spermathecae and drone semen. Expression of the mRNA encoding Alpha glucosidase 2 was higher in the spermathecae of virgin queens. Finally, expression of Facilitated trehalose transporter 1 mRNA was greatest in drone semen. This is the first characterization of gene expression in the spermathecae of honey bee queens revealing the alterations in mRNA levels within them after mating. Future studies will extend to other reproductive tissues with the purpose of relating levels of specific mRNAs to the functional competence of honey bee queens and the colonies they head.
Profiling and Functional Analysis of Long Noncoding RNAs and mRNAs during Porcine Skeletal Muscle Development

Gene transcripts or mRNAs and long noncoding RNAs (lncRNAs) are differentially expressed during porcine skeletal muscle development. However, only a few studies have been conducted on skeletal muscle transcriptome in pigs based on timepoints according to the growth curve for porcine. Here, we investigated gene expression in Qingyu pigs at three different growth stages: the inflection point with the maximum growth rate (MGI), the inflection point of the gradually increasing stage to the rapidly increasing stage (GRI), and the inflection point of the rapidly increasing stage to the slowly increasing stage (RSI). Subsequently, we explored gene expression profiles during muscle development at the MGI, GRI and RSI stages by Ribo-Zero RNA sequencing. Qingyu pigs reached the MGI, GRI and RSI stages at 156.40, 23.82 and 288.97 days of age with 51.73, 3.14 and 107.03 kg body weight, respectively. A total of 14,530 mRNAs and 11,970 lncRNAs were identified at the three stages, and 645, 323 differentially expressed genes (DEGs) and 696, 760 differentially expressed lncRNAs (DELs) were identified in the GRI vs. MGI, and RSI vs. MGI, comparisons. Functional enrichment analysis revealed that genes involved in immune system development and energy metabolism (mainly relate to amino acid, carbohydrate and lipid) were enriched at the GRI and MGI stages, respectively, whereas genes involved in lipid metabolism were enriched at the RSI stage. We further characterized G1430, an abundant lncRNA. The full-length sequence (316 nt) of lncRNA G1430 was determined by rapid amplification of cDNA ends (RACE). Subcellular distribution analysis by quantitative real-time PCR (qRT-PCR) revealed that G1430 is a cytoplasmic lncRNA. Binding site prediction and dual luciferase assay showed that lncRNA G1430 directly binds to microRNA 133a (miR-133a). Our findings provide the basis for further investigation of the regulatory mechanisms and molecular genetics of muscle development in pigs.
Steroid profiling of glucocorticoids in microdissected mouse brain across development

Corticosterone is produced by the adrenal glands and also produced locally by other organs, such as the brain. Local levels of corticosterone in specific brain regions during development are not known. Here, we microdissected brain tissue and developed a novel liquid chromatography tandem mass spectrometry method (LC‐MS/MS) to measure a panel of seven steroids (including 11‐deoxycorticosterone (DOC), corticosterone, and 11‐dehydrocorticosterone (DHC) in the blood, hippocampus (HPC), cerebral cortex (CC), and hypothalamus (HYP) of mice at postnatal day (PND) 5, 21, and 90. In a second cohort of mice, we measured the expression of three genes that code for steroidogenic enzymes that regulate corticosterone levels (Cyp11b1, Hsd11b1, and Hsd11b2) in the HPC, CC, and HYP. There were region‐specific patterns of steroid levels across development, including higher corticosterone levels in the HPC and HYP than in the blood at PND5. In contrast, corticosterone levels were higher in the blood than in all brain regions at PND21 and PND90. Brain corticosterone levels were not positively correlated with blood corticosterone levels, and correlations across brain regions increased with age. Local corticosterone levels were best predicted by local DOC levels at PND5, but by local DHC levels at PND21 and PND90. Transcripts for the three enzymes were detectable in all samples (with highest expression of Hsd11b1) and showed region‐specific changes with age. These data demonstrate that individual brain regions fine‐tune local levels of corticosterone during early development and that coupling of glucocorticoid levels across regions increases with age.
Performance of Winter-Sown Cereal Catch Crops after Simulated Forage Crop Grazing in Southland, New Zealand

(1) Background: Winter grazing of livestock poses significant environmental risks of nitrogen (N) leaching and sediment runoff. (2) Methods: A field study tested the effects of sowing catch crops of oats (Avena sativa L.), ryecorn (Secale cereale L.) or triticale (Triticosecale) in June and August (winter) in Southland, New Zealand (NZ), on the risk of N leaching losses from simulated N loads left after winter forage grazing. (3) Results: Catch crops took up 141–191 kg N ha−1 by green-chop silage maturity (approximately Zadoks growth stage 52; November/December). Importantly, early-sown catch crops were able to capture more N during the key leaching period from winter to mid-spring (77–106 kg N ha−1 cf. 27–31 kg N ha−1 for June and August treatments, respectively). At this time, ryecorn and triticale crops sown in June captured 20–29 kg ha−1 more N than June-sown oats (77 kg N ha−1). In October, early-sown catch crops reduced mineral N in the soil profile (0–45 cm depth) by 69–141 kg N ha−1 through the process of plant uptake. At green-chop silage maturity, catch crop yields ranged from 6.6 to 14.6 t DM ha−1. Highest yields and crop quality profiles (e.g., metabolizable energy, crude protein, soluble sugars and starch) were achieved by the oats, irrespective of the sowing date, indicating that trade-offs likely exist between environmental and productive performances of the catch crop species tested. (4) Conclusion: The catch crop of choice by farmers will depend on the desired end use for the crop, its place in the crop rotation and its potential for an environmental benefit.
Establishment of a Genome Editing Tool Using CRISPR-Cas9 in Chlorella vulgaris UTEX395

To date, Chlorella vulgaris is the most used species of microalgae in the food and feed additive industries, and also considered as a feasible cell factory for bioproducts. However, the lack of an efficient genetic engineering tool makes it difficult to improve the physiological characteristics of this species. Therefore, the development of new strategic approaches such as genome editing is trying to overcome this hurdle in many research groups. In this study, the possibility of editing the genome of C. vulgaris UTEX395 using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) has been proven to target nitrate reductase (NR) and adenine phosphoribosyltransferase (APT). Genome-edited mutants, nr and apt, were generated by a DNA-mediated and/or ribonucleoprotein (RNP)-mediated CRISPR-Cas9 system, and isolated based on the negative selection against potassium chlorate or 2-fluoroadenine in place of antibiotics. The null mutation of edited genes was demonstrated by the expression level of the correspondent proteins or the mutation of transcripts, and through growth analysis under specific nutrient conditions. In conclusion, this study offers relevant empirical evidence of the possibility of genome editing in C. vulgaris UTEX395 by CRISPR-Cas9 and the practical methods. Additionally, among the generated mutants, nr can provide an easier screening strategy during DNA transformation than the use of antibiotics owing to their auxotrophic characteristics. These results will be a cornerstone for further advancement of the genetics of C. vulgaris.
Looking for Abnormalities in Mammograms With Self- and Weakly Supervised Reconstruction

Early breast cancer screening through mammography produces every year millions of images worldwide. Despite the volume of the data generated, these images are not systematically associated with standardized labels. Current protocols encourage giving a malignancy probability to each studied breast but do not require the explicit and burdensome annotation of the affected regions. In this work, we address the problem of abnormality detection in the context of such weakly annotated datasets. We combine domain knowledge about the pathology and clinically available image-wise labels to propose a mixed self- and weakly supervised learning framework for abnormalities reconstruction. We also introduce an auxiliary classification task based on the reconstructed regions to improve explainability. We work with high-resolution imaging that enables our network to capture different findings, including masses, micro-calcifications, distortions, and asymmetries, unlike most state-of-the-art works that mainly focus on masses. We use the popular INBreast dataset as well as our private multi-manufacturer dataset for validation and we challenge our method in segmentation, detection, and classification versus multiple state-of-the-art methods. Our results include image-wise AUC up to 0.86, overall region detection true positives rate of 0.93, and the pixel-wise ${F}_{{1}}$ score of 64% on malignant masses.
The Compass of Shame Scale: Dimensionality and Gender Measurement Invariance in a Portuguese Sample

Abstract Different shame coping strategies directly influence shame experience. However, the assessment of these strategies has received little attention. The Compass of Shame Scale (CoSS-5) was developed to assess shame-coping styles and has proven to be a valid measure. In this study, the CoSS-5 dimensionality was examined throughout confirmatory analysis and its measurement invariance across gender was investigated for the original (four-factor) and a new recently proposed measurement model (five-factor). The sample consisted of 605 Portuguese adults (57.5% female; Mean age = 35.43) recruited from the community. In addition to the CoSS-5, participants completed other measures relevant for construct validity. Both the four and the five-factor measurement models revealed a good fit to the data. Good reliability values were found for all factors, with Cronbach’s alphas ranging between . 79 and . 90. The CoSS-5 also proved to be gender invariant, regardless of the measurement model. The subscales of CoSS-5 associated in the expected direction with measures of external shame, self-critical and self-reassuring responses, psychological flexibility/inflexibility, and psychopathology symptoms. This study highlights the relevance of the CoSS-5 in advancing knowledge on the impact of shame-coping styles on psychopathological outcomes, making it available for use in research and clinical settings.
Lenvatinib for Hepatocellular Carcinoma: A Literature Review

Lenvatinib, which is an oral multikinase inhibitor, showed non-inferiority to the sorafenib in terms of overall survival (OS) and a higher objective response rate (ORR) and better progression-free survival (PFS) in patients with hepatocellular carcinoma (HCC). A good liver function and Barcelona Clinic Liver Cancer (BCLC) intermediate stage were the key factors in achieving therapeutic efficacy. The management of adverse events plays an important role in continuing lenvatinib treatment. While sequential therapies contributed to prolonging overall survival, effective molecular targeted agents for the administration after lenvatinib have not been established. Repeated transcatheter arterial chemoembolization (TACE) was associated with a decline in the liver function and poor therapeutic response in BCLC intermediate patients. Recently, the Asia-Pacific Primary Liver Cancer Expert (APPLE) Consensus Statement proposed the criteria for TACE unsuitability. Upfront systemic therapy may be better for the BCLC intermediate stage HCC patients with a high tumor burden, while selective TACE will be recommended for obtaining a curative response in patients with a low tumor burden. This article reviews the therapeutic response, management of adverse events, post-progression treatment after Lenvatinib, and treatment strategy for BCLC intermediate stage HCC.
Periaortic Ventricular Tachycardias in Nonischemic Cardiomyopathy: Substrate and Electrocardiographic Correlations.

Background - Periaortic fibrotic ventricular tachycardia (VT) substrate is common in nonischemic cardiomyopathy (NICM), often intramural, and difficult to ablate. We sought to better characterize normal and abnormal periaortic voltage map parameters and NICM periaortic VTs. Methods - In 15 patients without heart disease, the 5th percentile of endocardial voltage for increasing distance from the aortic valve ring was determined. In 53 consecutive NICM patients (64±11 years; left ventricular ejection fraction 31±10%) undergoing ablation of recurrent VT, periaortic electrogram voltage and VT characteristics were analyzed. Results - In healthy patients, the 5th percentile of the bipolar voltage (BV) increased proportional to the distance from the aortic valve ring, from 1.0mV at 1cm to 1.5mV at 1.5cm; the corresponding unipolar voltage (UV) cutoffs were 5.0mV and 7.5mV. A total of 160 VTs were induced in 53 NICM patients, of which 28 VTs in 20 patients had periaortic origins. Periaortic VTs were associated with similar periaortic BV, but lower UVs consistent with intramural fibrosis as an important substrate. Periaortic VTs could be divided into left (LBBB) and right (RBBB) bundle branch block forms with mapping showing right septal and lateral exits. LBBB VTs were more often acutely abolished with ablation (100% vs. 69%; p=0.034), but with a 23% incidence of heart block. Greater extent of low voltage was associated with more induced VTs and worse acute outcome. Conclusions - Adjusting voltage parameters based on distance from the aortic valve may improve definition of LVOT arrhythmia substrate. Periaortic VTs are common in NICM, often associated with intramural substrate and can be divided into LBBB and RBBB types associated with different ablation outcomes and risks.
Trends on Aspergillus Epidemiology—Perspectives from a National Reference Laboratory Surveillance Program

Identification of Aspergillus to species level is important since sibling species may display variable susceptibilities to multiple antifungal drugs and also because correct identification contributes to improve the knowledge of epidemiological studies. Two retrospective laboratory studies were conducted on Aspergillus surveillance at the Portuguese National Mycology Reference Laboratory. The first, covering the period 2017–2018, aimed to study the molecular epidemiology of 256 Aspergillus isolates obtained from patients with respiratory, subcutaneous, or systemic infections and from environmental samples. The second, using our entire collection of clinical and environmental A. fumigatus isolates (N = 337), collected between 2012 and 2019, aimed to determine the frequency of azole-resistant A. fumigatus isolates. Aspergillus fumigatus sensu stricto was the most frequent species in both clinical and environmental samples. Overall, and considering all Aspergillus sections identified, a high frequency of cryptic species was detected, based on beta-tubulin or calmodulin sequencing (37% in clinical and 51% in environmental isolates). Regarding all Fumigati isolates recovered from 2012–2019, the frequency of cryptic species was 5.3% (18/337), with the identification of A. felis (complex), A. lentulus, A. udagawae, A. hiratsukae, and A. oerlinghauensis. To determine the frequency of azole resistance of A. fumigatus, isolates were screened for azole resistance using azole-agars, and 53 possible resistant isolates were tested by the CLSI microdilution reference method. Nine A. fumigatus sensu stricto and six Fumigati cryptic isolates showed high minimal inhibitory concentrations to itraconazole, voriconazole, and/or posaconazole. Real-time PCR to detect cyp51A mutations and sequencing of cyp51A gene and its promoter were performed. The overall frequency of resistance to azoles in A. fumigatus sensu stricto was 3.0%. With this retrospective analysis, we were able to detect one azole-resistant G54R mutant A. fumigatus environmental isolate, collected in 2015. The TR34/L98H mutation, linked to environmental transmission route of azole resistance, was the most frequently detected mutation (N = 4; 1.4%). Our findings underline the demand for correct identification and susceptibility testing of Aspergillus isolates.