Miracle-dz/UniFAR-FTUs huggingface dataset:
Immunohistochemical Study of Ceruloplasmin, Lactoferrin and Secretory Component Expression in Neoplastic and Non-Neoplastic Thyroid Gland Diseases

The expression of lactoferrin (LF), ceruloplasmin (CP) and secretory component (SC) in various thyroid diseases was investigated using an immunohistochemical method. LF is an iron-binding protein overexpressed in certain human adenocarcinomas. CP is implicated in carcinogenesis and SC is part of the local immune defense system. Sections of 15 follicular adenomas, 5 follicular carcinomas (FC), 16 papillary carcinomas (PC), 15 goitres and 2 cases of lymphocytic thyroiditis were re-examined and investigated immunohistologically by a streptavidin-biotin method. A positive immunostain reaction for CP and LF was observed in all cases of PC and FC. A positive immunostain reaction for SC was observed in 13/16 cases of PC and 4/5 cases of FC. A negative immunostain reaction for CP, LF and SC was observed in medullary cancers, in follicular adenomas and in the cases with goitre. We conclude that the immunostain expression of LF, CP and SC is a valuable diagnostic aid in the differential diagnosis between benign and malignant thyroid tumors. The expression of SC indicates the presence of a local immunodefensive system.
Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens

DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters. The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota. In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics. The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens. In ovo stimulation was performed on day 12 of egg incubation. Bioactive compounds delivered in ovo included (S1)—Lactobacillus salivarius with GOS and (S2)—Lactobacillus plantarum with RFO. Samples were collected from six individuals from each group on day 42 post-hatching. DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method. Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery. The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
Factors Affecting Gross Manual Dexterity: A Structural Equation Modeling Approach

Gross manual dexterity is an important part of human factors and ergonomics. The purpose of the current study was to investigate factors affecting gross manual dexterity. Gender, palm length, grip strength, hand skin temperature, room temperature, and room humidity were analyzed simultaneously to predict gross manual dexterity by utilizing Structural Equation Modeling (SEM) approach. SEM showed that hand skin temperature was the most important factor for predicting gross manual dexterity, followed by several other factors such as grip strength, gender, and room temperature. In addition, room humidity, age, and hand size were also had significant indirect effects on gross manual dexterity. The SEM derived in this study could be a very valuable theoretical foundation which could be very beneficial for human factors engineer, physical therapist, and even medical doctors.
Results of Treatment for Patients With Multicentric or Bilaterally Predisposed Unilateral Wilms Tumor (AREN0534): A report from the Children's Oncology Group

A primary objective of Children's Oncology Group study AREN0534 (Treatment for Patients With Multicentric or Bilaterally Predisposed, Unilateral Wilms Tumor) was to facilitate partial nephrectomy in 25% of children with bilaterally predisposed unilateral tumors (Wilms tumor/aniridia/genitourinary anomalies/range of developmental delays [WAGR] syndrome; and multifocal and overgrowth syndromes). The purpose of this prospective study was to achieve excellent event‐free survival (EFS) and overall survival (OS) while preserving renal tissue through preoperative chemotherapy, completing definitive surgery by 12 weeks from diagnosis, and modifying postoperative chemotherapy based on histologic response.
Clinical characteristics of moderate–severe obsessive–compulsive disorder in children and adolescents in China

Objective This study reports clinical characteristic of moderate–severe obsessive–compulsive disorder (OCD) among school students in China. Methods We examined 153 patients for the distribution of OCD symptoms using the Yale-Brown Obsessive Compulsive Scale Symptoms Checklist, the severity of anxiety and depression symptoms using the Hamilton Anxiety Scale and the Hamilton Depression Scale-24, respectively, and impairment in learning, family and social functions using the Pediatric Quality of Life Enjoyment and Satisfaction Questionnaire. Results The number of total OCD, obsession and compulsion symptoms was 6.71 (standard deviation [SD] = 2.25), 3.77 (SD = 1.32) and 2.94 (SD = 1.59), respectively. The incidence of moderate and severe depressive symptoms for junior high school students was significantly higher than for primary and high school students. The number of children and adolescents with OCD increased with age, reaching a peak in the senior high school stage. Conclusion The most common symptoms in children and adolescent OCD patients are miscellaneous obsessions, aggressiveness, religiousness, checking, miscellaneous compulsions, cleaning-washing and repeating. These patients show a relatively high co-occurrence rate of anxiety symptoms and depressive symptoms, which impairs their learning, as well as their family and social functions.
Inclusion of oxidized fish oil in mink diets 1. The influence on nutrient digestibility and fatty‐acid accumulation in tissues

Zusammenfassung Der Einsatz von oxidiertem Fischol im Nerzfutter. 1. Der Einflus auf die Verdaulichkeit der Nahrstoffe und die Einlagerung von Fettsauren in verschiedenen Geweben In einem Futterungsversuch mit 18 ausgewachsenen, mannlichen Pastelnerzen uber die Dauer von 15 Wochen, wurde der Einflus hoher Konzentrationen (55% der metabolisierbaren Energie) von frischem und oxidiertem Fischol (200 and 400 maqu. O2/kg Ol) auf das Wachstum sowie die Fettsaureeinlagerung in Leber und Inguinalfett untersucht. Der Versuch schlos eine Bestimmung der scheinbaren Verdaulichkeiten von Nahrstoffen, Fettsauren und α-Tocopherol in der achten Versuchswoche ein. Die Qualitat des eingesetzten Fischols wurde wahrend der Oxidation und Lagerung (13 Wochen, —80°C unter Licht- und Sauerstoffabschlus) fortlaufend uberpruft. Wahrend der Lagerung konnten die Peroxidzahlen in den eingesetzten Fischolen relativ stabil gehalten werden. Es wurde jedoch ein erheblicher Verlust an n-3 Fettsauren insbesondere in dem stark oxidierten Ol (400 maqu. O2/kg Ol) beobachtet. Die scheinbare Fettverdaulichkeit nahm von 95% (frisches Fischol) auf 91% (200 maqu. O2/kg Ol) und auf 74% (400 maqu. O2/kg Ol) ab. Parallel hierzu nahm die scheinbare Verdaulichkeit der Gesamtfettsauren ab. Die scheinbare Verdaulichkeit von α-Tocopherol war nicht von der Qualitat des Futterfettes beeinflust und lag bei 60%. Die Futteraufname und das Wachstum der Nerze war insbesondere durch die Verabreichung des ranzigen Fettes erheblich beeintrachtigt. Die Herkunft des Futterfettes (marines Ol) spiegelte sich deutlich in der Fettsaurezusammensetzung des Leber- und Inguinalfettes wider. In den untersuchten Geweben wurde eine erhebliche Einlagerung von langkettigen Fettsauren (C20, C22) beobachtet (Leber 28–31% der Gesamtfettsauren; Inguinalfett 38–42% der Gesamtfettsauren).
The Role of LIN28-let-7-ARID3B Pathway in Placental Development

Placental disorders are a major cause of pregnancy loss in humans, and 40–60% of embryos are lost between fertilization and birth. Successful embryo implantation and placental development requires rapid proliferation, invasion, and migration of trophoblast cells. In recent years, microRNAs (miRNAs) have emerged as key regulators of molecular pathways involved in trophoblast function. A miRNA binds its target mRNA in the 3ʹ-untranslated region (3ʹ-UTR), causing its degradation or translational repression. Lethal-7 (let-7) miRNAs induce cell differentiation and reduce cell proliferation by targeting proliferation-associated genes. The oncoprotein LIN28 represses the biogenesis of mature let-7 miRNAs. Proliferating cells have high LIN28 and low let-7 miRNAs, whereas differentiating cells have low LIN28 and high let-7 miRNAs. In placenta, low LIN28 and high let-7 miRNAs can lead to reduced proliferation of trophoblast cells, resulting in abnormal placental development. In trophoblast cells, let-7 miRNAs reduce the expression of proliferation factors either directly by binding their mRNA in 3ʹ-UTR or indirectly by targeting the AT-rich interaction domain (ARID)3B complex, a transcription-activating complex comprised of ARID3A, ARID3B, and histone demethylase 4C (KDM4C). In this review, we discuss regulation of trophoblast function by miRNAs, focusing on the role of LIN28-let-7-ARID3B pathway in placental development.
HDAC3–ERα Selectively Regulates TNF-α-Induced Apoptotic Cell Death in MCF-7 Human Breast Cancer Cells via the p53 Signaling Pathway

Tumor necrosis factor-α (TNF-α) plays a significant role in inflammation and cancer-related apoptosis. We identified a TNF-α-mediated epigenetic mechanism of apoptotic cell death regulation in estrogen receptor-α (ERα)-positive human breast cancer cells. To assess the apoptotic effect of TNF-α, annexin V/ propidium iodide (PI) double staining, cell viability assays, and Western blotting were performed. To elucidate this mechanism, histone deacetylase (HDAC) activity assay and immunoprecipitation (IP) were conducted; the mechanism was subsequently confirmed through chromatin IP (ChIP) assays. Finally, we assessed HDAC3–ERα-mediated apoptotic cell death after TNF-α treatment in ERα-positive human breast cancer (MCF-7) cells via the transcriptional activation of p53 target genes using luciferase assay and quantitative reverse transcription PCR. The TNF-α-induced selective apoptosis in MCF-7 cells was negatively regulated by the HDAC3–ERα complex in a caspase-7-dependent manner. HDAC3 possessed a p53-binding element, thus suppressing the transcriptional activity of its target genes. In contrast, MCF-7 cell treatment with TNF-α led to dissociation of the HDAC3–ERα complex and substitution of the occupancy on the promoter by the p53–p300 complex, thus accelerating p53 target gene expression. In this process, p53 stabilization was accompanied by its acetylation. This study showed that p53-mediated apoptosis in ERα-positive human breast cancer cells was negatively regulated by HDAC3–ERα in a caspase-7-dependent manner. Therefore, these proteins have potential application in therapeutic strategies.
K-SPIN: Efficiently Processing Spatial Keyword Queries on Road Networks : (Extended Abstract)

Given the prevalence and volume of local search queries, today’s search engines are required to find results by both spatial proximity and textual relevance at high query throughput. Existing techniques to answer such spatial keyword queries employ a keyword aggregation strategy that suffers from certain drawbacks when applied to road networks. Instead, we propose the K-SPIN framework, which uses an alternative keyword separation strategy that is more suitable on road networks. While this strategy was previously thought to entail prohibitive pre-processing costs, we further propose novel techniques to make our framework viable and even light-weight. Thorough experimentation shows that K-SPIN outperforms the state-of-the-art by up to two orders of magnitude on a wide range of settings and real-world datasets.
Supplemental skim milk before and after weaning improves growth performance of pigs

Twelve Large White ◊ Landrace sows nursing litters of 6 boars and 6 gilts were used in this study. Six litters suckled the sow only, whereas the other 6 litters received supplemental liquid skim milk (200 g/L) ad libitum from Day 10 until Day 20. On Day 20 the 4 heaviest pigs of each sex were allocated to 2 pairs and were weaned. Each pair was offered either pelleted or pelleted plus liquid feed. For the first 2 days post-weaning, each pair of liquid-supplemented pigs received liquid skim milk (250 g/L). On Day 23, pelleted feed was added to the milk. The ratio of liquid to pelleted feed was adjusted daily until, on Day 28, pigs were provided with pelleted feed only until 41 days of age. Supplemental skim milk increased growth (223 v. 291 g/day, P < 0.001) between Days 10 and 20 of age so that by weaning supplemented pigs were 10% (6.13 v. 6.74 kg, P = 0.038) heavier than unsupplemented pigs. Skim milk intake increased linearly from 190 to 600 g/day per pig over the 10 days of supplementation. Supplemental milk feeding did not alter sow liveweight change (n31.9 v. n30.3 kg for sows nursing litters with and without supplementation, respectively, P = 0.894) or change in P2 backfat thickness (n5.3 v. n4.2 mm, P = 0.279) between farrowing and Day 20 of age. Pigs weaned onto skim milk and pellets ate more (257 v. 30 g dry matter (DM)/day, P < 0.001) and grew better (213 v. n151 g/day, P < 0.001) over the first 2 days post-weaning than pigs weaned onto only pellets. Piglets provided with liquid feed after weaning continued to grow faster beyond Day 22, resulting in the benefit of weaning onto liquid feed being maintained until at least 41 days of age (14.1 v. 12.8 kg, P < 0.001). In conclusion, skim milk feeding before and after weaning can result in cumulative improvements in growth performance in the nursery.
Effects of Growth Hormone Plus a Hyperproteic Diet on Methotrexate-induced Injury in Rat Intestines

The aim of this study was to determine whether growth hormone treatment reduces injury to the intestinal mucosa induced by methotrexate (MTX). Wistar rats with intestinal injury induced by methotrexate were treated with daily growth hormone, beginning 3 days before MTX treatment until 3 or 4 days after MTX administration. The rats were killed at 3 or 7 days post-MTX administration. The rats were fed with either a normoproteic diet or a hyperproteic diet. Body weight, mortality, bacterial translocation, intestinal morphometry, proliferation and apoptosis and blood somatostatin and IGF-1 were determined. Combined administration of growth hormone and a hyperproteic diet reduces MTX-induced mortality. This effect was accompanied by increased cell proliferation and decreased apoptosis within the crypt. Morphometric data showed complete recovery of the mucosa by day 7 post-MTX administration. These results indicate a synergistic protective action of growth hormone combined with a hyperproteic diet to MTX-induced injury.
Exponential nutrient loading shortens the cultural period of Larix olgensis seedlings

Abstract Traditional nursery culture of Changbai larch (Larix olgensis Henry) in Northeast China requires up to a 2-year nursery production period. The high cost of seedling culture for this duration has been identified as a constraint. As a potential means to help to shorten the cultural period, we evaluated growth, biomass accumulation, and nitrogen (N) uptake dynamics of 1-year-old seedlings subjected to nutrient-loading treatments at intensive rates of 500 mg N seedling−1 supplied exponentially (E500) and 135 mg N seedling−1 supplied exponentially (E135) or conventionally (constant). Also included were an unfertilized control and the standard fertilizer regime for 2-year-old seedling production (77 mg N seedling−1 supplied conventionally over 2 years). At the end of nursery culture, though seedlings in the constant treatment had more biomass and N accumulation at the whole-seedling level, much of this was allocated to needles. While seedlings in the E500 treatment exhibited signs of toxicity, those in the E135 treatment had equivalent end-of-nursery N concentration in woody tissues to those produced over 2 years; root collar increment and survival 69 d after outplanting were also similar. Our results suggest that exponential nutrient loading at an optimal rate could increase available nutrient reserves in woody tissues of deciduous tree seedlings, therefore having the potential to improve seedling performance after outplanting.
Detection of Coxiella burnetii and equine herpesvirus 1, but not Leptospira spp. or Toxoplasma gondii, in cases of equine abortion in Australia - a 25 year retrospective study

Equine abortion is a cause of severe economic loss to the equine industry. Equine herpesvirus 1 is considered a primary cause of infectious abortion in horses, however other infectious agents can also cause abortion. Abortions due to zoonotic pathogens have implications for both human and animal health. We determined the prevalence of Coxiella burnetii, Leptospira spp. and Toxoplasma gondii in 600 aborted equine foetal tissues that were submitted to our diagnostic laboratories at the University of Melbourne from 1994 to 2019. Using qPCR we found that the prevalence of C. burnetii was 4%. The highest annual incidence of C. burnetii was observed between 1997–2003 and 2016–2018. The prevalence of C. burnetii in Victoria and New South Wales was 3% and 6% respectively. All the samples tested negative for Leptospira spp. and Toxoplasma gondii DNA. Equine herpesvirus 1 DNA was detected at a prevalence of 3%. This study has provided evidence for the presence of C. burnetii in equine aborted foetal tissues in Australia, but the role of C. burnetii as potential cause of abortion in Australia requires further investigation. C. burnetii is a zoonotic disease agent that causes the disease ‘Q fever’ in humans. We recommend that appropriate protective measures should be considered when handling material associated with equine abortions to reduce the risk of becoming infected with C. burnetii.
PINNIPED LACTATION STRATEGIES: EVALUATION OF DATA ON MATERNAL AND OFFSPRING LIFE HISTORY TRAITS

Interspecific correlations are commonly used to explore the adaptive functions of life history traits in pinnipeds. Although adaptive conclusions are improved by the use of comparative methods that account for underlying phylogenetic relationships among species, they are still dependent on the quality of life history data. We collected pinniped species estimates for 12 maternal and offspring life history traits and evaluated these estimates based on sample size, duration of study, and methods used to obtain the data. Although excellent data exist for some species, high-quality estimates in all 33 species are not available for any of the traits studied. High-quality estimates of maternal postpartum mass are known for 12 species, neonate birth mass for 21, pup rate of mass gain for 12, lactation length for 10, and weaning mass for 10. High-quality estimates of milk composition, milk energy output, and maternal foraging behavior during lactation are limited to ≤ 50% of species, with a taxonomic bias favoring the larger phocid species. Obtaining data on small-bodied phocid species will be critical to gaining a better understanding of the relative roles of body size and phylogeny in the evolution of pinniped lactation strategies.
Thrombus in the left atrial appendage in patients with atrial fibrillation treated with non‐vitamin K antagonist oral anticoagulants in clinical practice—A multicenter registry

The prevalence and predictors of left atrial appendage thrombus (LAAT) in patients with non‐valvular atrial fibrillation (AF) who have been treated with non‐vitamin K antagonist oral anticoagulants (NOACs) are not well defined. We aimed to assess the occurrence and predictors of LAAT on transesophageal echocardiography (TOE) in patients with non‐valvular AF treated with NOACs for at least 3 weeks.
The isolation and identification of microbes from a fermented tea beverage, Haipao, and their interactions during Haipao fermentation

Samples of Haipao from three cities of Taiwan were analyzed for their microbial population. Two species of acetic acid bacteria and three species of yeast were isolated from tea fungus Haipao using appropriate isolation media. The isolated bacteria were identified asAcetobacter acetiandAcetobacter pasteurianus, based on their biochemical properties, and compared with those of the type strains of the genusAcetobacter. The yeasts wereSaccharomyces cerevisiae, Zygosaccharomyces bailii, andBrettanomyces bruxellensisaccording to conventional phenotypic characterization combined with the Yeast Identification Program. The brew broth analyzed by high−performance liquid chromatography (HPLC) was shown to contain glycerol, acetic acid and ethanol. The symbiosis phenomenon between the yeast andAcetobacterwas studied. It was found that the autoclaved yeast cells and ethanol produced by yeasts were helpful forAcetobacterto grow or produce acetic acid. The acetic acid produced byAcetobactercould stimulate the yeast to produce ethanol. The ethanol and acetic acid produced by yeasts andAcetobactermight prevent the competition from other micro-organisms.
Risk of Bleeding with Exposure to Warfarin and Nonsteroidal Anti-Inflammatory Drugs: A Systematic Review and Meta-Analysis

Abstract Background Warfarin use can trigger the occurrence of bleeding independently or as a result of a drug–drug interaction when used in combination with nonsteroidal anti-inflammatory drugs (NSAIDs). Objectives This article examines the risk of bleeding in individuals exposed to concomitant warfarin and NSAID compared with those taking warfarin alone (Prospero Registry ID 145237). Methods PubMed, EMBASE, Scopus, and Web of Science were searched. The primary outcome of interest was gastrointestinal bleeding and general bleeding. Summary effects were calculated to estimate average treatment effect using random effects models. Heterogeneity was assessed using Cochran's Q and I 2. Risk of bias was also assessed using the Agency for Healthcare Research and Quality bias assessment tool. Results A total of 651 studies were identified, of which 11 studies met inclusion criteria for meta-analysis. The odds ratio (OR) for gastrointestinal bleeding when exposed to warfarin and an NSAID was 1.98 (95% confidence interval [CI]: 1.55–2.53). The risk of gastrointestinal bleeding was also significantly elevated with exposure to a COX-2 inhibitor and warfarin relative to warfarin alone (OR = 1.90, 95% CI: 1.46–2.46). There was an increased risk of general bleeding with the combination of warfarin with NSAIDs (OR = 1.58, 95% CI: 1.18–2.12) or COX-2 inhibitors (OR = 1.54, 95% CI: 0.86–2.78) compared with warfarin alone. Conclusion Risk of bleeding is significantly increased among persons taking warfarin and a NSAID or COX-2 inhibitor together as compared with taking warfarin alone. It is important to caution patients about taking these medications in combination.