Miracle-dz/UniFAR-FTUs huggingface dataset:
Edinburgh Research Explorer Relative contributions of adipose-resident CD146 pericytes and CD34 adventitial progenitor cells in bone tissue engineering

Pericytes and other perivascular stem/stromal cells are of growing interest in the ﬁ eld of tissue engineering. A portion of perivascular cells are well recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Here, we investigate the differential but overlapping roles of two perivascular cell subsets in paracrine induction of bone repair. CD146 + CD34 − CD31 − CD45 − pericytes and CD34 + CD146 − CD31 − CD45 − adventitial cells were derived from human adipose tissue and applied alone or in combination to calvarial bone defects in mice. In vitro, osteogenic differentiation and tubulogenesis assays were performed using either ﬂ uorescence activated cell sorting-derived CD146 + pericytes or CD34 + adventitial cells. Results showed that CD146 + pericytes induced increased cord formation in vitro and angiogenesis in vivo in comparison with patient-matched CD34 + adventitial cells. In contrast, CD34 + adventitial cells demonstrated heightened paracrine-induced osteogenesis in vitro. When applied in a critical-size calvarial defect model in NOD/SCID mice, the combination treatment of CD146 + pericytes with CD34 + adventitial cells led to greater re-ossi ﬁ cation than either cell type alone. In summary, adipose-derived CD146 + pericytes and CD34 + adventitial cells display functionally distinct yet overlapping and complementary roles in bone defect repair. Consequently, CD146 + pericytes and CD34 + adventitial cells may demonstrate synergistic bone healing when applied as a combination cellular therapy. npj Medicine ;
Study of Relative Frequency and Spectrum of Chlorophyll Mutants in M2 Generation for Facilitating Mutation Breeding in Chickpea

Chick pea is the second largest grown food legume of the world. It ranks third among pulses, fifth among grain legumes and 19th among grain crops of the world. Chickpea is considered as an important legume for human diet since ancient times. It is good source of protein, carbohydrates and vitamins B complex, which are essential components of healthy diet Umavathi, et al. [1]. The existing chickpea germplasm has narrow genetic base for improvement of economic traits Tariq, et al. [2]. Among the conventional plant breeding strategies, mutation breeding is widely trusted method for creation of variability Elangovan, et al. [3]. Induced mutagenesis by using different physical and chemical mutagens is a sophisticated and important approach to create desirable variability which ultimately results in creation of new varieties with improved and unique characteristics Monica, et al. [4]. Induced mutation ultimately increase the chance of getting desirable variation which helps to accelerate the breeding process more efficiently as compared to normal breeding practices for getting novelties Navnath [5]. Mutation can also be used for correcting undesirable traits or to get novel combinations. To obtain new combinations is highly desirable without affecting the remaining major portion of genetic makeup Navnath, et al. [6]. Gamma rays and EMS are more frequently and efficiently used physical and chemical mutagens respectively with ability to produce higher frequency of mutation in higher plants Monica, et al. [4]. Chickpea being an important pulse crop attracts the plant breeders to reconstruct it genetically Samiullah, et al. [7]. Chlorophyll mutations are widely used for the potential assessment of different mutagens in creating genetic variability. In M2 generation, frequency of chlorophyll mutants is considered as a standard measure of mutation rate. For any effective mutation breeding program, selection of effective and efficient mutagen is very essential to receive high frequency of desirable mutations Solanki, et al. [8]. Hence, the basic information on mutagen dose, efficiency of mutagens, methods of handling the material and treatment methods required to maximize mutation induction is essential for success in any mutation breeding program. The present study was undertaken to understand the response of ARTICLE INFO AbsTRACT
Thermodynamic Analysis of Continuous Vibrating Fluidised Bed Drying of Grain

The performance of continuous vibrating fluidised bed drying of corn grits was simulated (using a previously validated mathematical model) and analysed based on the first- and second law of thermodynamics. The energy and exergy analyses were performed for several drying conditions. The effects of inlet air velocity and temperature, vibration intensity, and particle size on the efficiencies and inefficiencies of drying process have been simulated and discussed. Generally, application of vibration during fluidised bed drying enhanced the drying process. The application of higher levels of drying air temperature and velocity led in higher exergy efficiencies and energy utilisation. Also, the exergy loss had maximum value when higher drying air temperature and velocity were used for the drying process.
Review Driving Apart and Segregating Genomes in Archaea

Genome segregation is a fundamental biological process in organisms from all domains of life. How this stage of the cell cycle unfolds in Eukarya has been clearly de ﬁ ned and considerable progress has been made to unravel chromosome partition in Bacteria. The picture is still elusive in Archaea. The lineages of this domain exhibit different cell-cycle lifestyles and wide-ranging chromosome copy numbers, ﬂ uctuating from 1 up to 55. This plurality of patterns suggests that a variety of mechanisms might underpin disentangling and delivery of DNA molecules to daughter cells. Here I describe recent developments in archaeal genome maintenance, including investigations of novel genome segregation machines that point to unforeseen bacterial and eukaryotic connections.
The Effect of Triage Education by Pre Advance-Organizer Model on the Knowledge Staff Disaster Team Nurses

Introduction: In the event of health crisis, optimum performance crisis team personnel, are necessary and expected, and the key to success in managing the large number of victims with limited resources is triage; therefore triage training of crisis team nurses is very important. Objective: The purpose of this study was to determine the effect of triage education by pre-advance organizer model on knowledge of crisis team nurses at AJA selected hospitals. Materials and Methods: In this quasi-experimental study, during year 2015, two hospitals affiliated to the army, based on the centrality of the crisis, were considered as the study population and assigned to intervention and control groups, 50 nurses from the two hospitals were selected, based on available sampling. Educational content triage in crisis by the pre-advance organizer model in two sessions for an hour and a half was held for the experimental group while the control group did not receive any intervention. For both groups, pretest and post test tools, demographic questionnaire, and knowledge questionnaire were used. Finally data were analyzed by using descriptive analytic statistics using the SPSS version 22 software. Results: The results showed that the mean knowledge of nurses before the intervention in the experimental group was 8.66±1.65 and in the control group was 7.57±1.19, respectively (p=0/264) , and after the intervention, in the experimental group it increased to 15.66 ±1.02 and in the control group to 8.3±0.92; the independent T-test showed a difference between the two groups, which was statistically meaningful (P<0/001). Discussion and Conclusion: Considering the positive results, the use of pre-advance organizing model to promote awareness of crisis team nurses, and subject triage crisis training for faculty of nursing and retraining workshops are recommended.
ACUTE PHASE REACTANT CORRELATES AMONG PREGNANT WOMEN IN PORT HARCOURT, NIGERIA

C-SJIF ABSTRACT Pregnancy is usually associated with many changes in a woman which can be physiologic or pathologic. This was a cross sectional study that was carried out with the aim of determining the acute phase reactant correlates among pregnant women in Port Harcourt, Nigeria. A total of 180 subjects were sampled, comprising of 90 pregnant and 90 non-pregnant women, within the age range of 16 to 45 years. Five milliliters of whole blood was collected from each subject at ambient temperature using standard venepuncture technique. Three milliliters was dispensed in EDTA bottle and was used for ESR assay, while 2 mililiters which was stored in plain bottle was used for CRP and albumin assays. C-reactive protein was analysed with reagents from BioCheck Inc, CA, USA using ELISA; ESR was analysed with Westergreen method; and Albumin was analysed with Bromocresolgreen method. The data obtained was coded and analysed using SPSS version 20. Comparison of pregnant women with controls using t-test, showed significant increase in ESR (10.97 vs 38.59 mm/hr, p <0.01) and CRP (0.000012 vs 0.00019 g/l, p <0.01), and decrease in albumin (45.80 vs 38.82 g/l, p<0.01). Comparison of the mean for the parameters within the three trimesters was done using ANOVA, and only albumin significantly varied (F=3.45, p=0.04). To identify the trimester pairs that had significant mean difference, Tukey’s Post Hoc test was used. Albumin had a significant mean difference between the 1 st and 3 rd trimesters (p= 0.04). Using Pearson’s correlation coefficient, ESR (r=0.47, p< 0.001) and CRP (r= -0.25, p< 0.01) negatively correlate with albumin; while ESR and CRP positively correlate with each other (r= 0.36, p< 0.01). Also, there was positive correlation of ESR and CRP in 2 nd trimester (r=0.41, p=0.03). At least one acute phase reactant should be incorporated as a routine pregnancy screening test in conjunction with clinical assessment for prompt and efficient diagnosis of inflammatory disorders.
empirical Self-Reports Versus Parental Perceptions of Health-Related Quality of Life Among Deaf Children and Adolescents

The aim of this study was to assess the agreement between deaf children’s and adolescents’ self-ratings of health-related quality of life (HRQoL) and their parents’ proxy reports. This observational cross-sectional study included 114 deaf 8- to 18-years-old students and proxy family members. HRQoL was measured using the KIDSCREEN-27 questionnaire, which was adapted to Spanish sign language for children, with a written version for parents. Respondents completed a self-administered paper questionnaire. Parents’ and children’s mean scores differences were not significant, except for the “Autonomy and Parents” and “Peers and Social Support” dimensions. Children aged 8–11 years scored higher in some domains of QoL compared to those aged 12–18 years. The level of agreement between children/adolescents’ and parents/ proxies’ responses was acceptable, except for the dimension “Autonomy and Parents. ” Overall, deaf children/adolescents’ self-ratings of HRQoL did not differ from their parents’ proxy reports; however, differences were found in the dimensions that explored the quality of the interaction of children/adolescents and parents,
Identiﬁed Challenges from Faculty Teaching at Predominantly Undergraduate Institutions after Abrupt Transition to Emergency Remote Teaching during the COVID-19 Pandemic

: COVID-19 has been one of the most signiﬁcant disruptors of higher education in modern history. Higher education institutions rapidly transitioned to Emergency Remote Teaching (ERT) in mid-to-late March of 2020. The extent of COVID-19’s impact on teaching and learning, and the resulting challenges facilitating ERT during this time, likely varied by faculty, institutional, and geographical characteristics. In this study, we identiﬁed challenges in teaching and learning during the initial transition to ERT at Predominantly Undergraduate Institutions (PUIs) in the Midwest, United States. We conducted in-depth interviews with 14 faculty teaching at Midwestern PUIs to explore their lived experiences. We describe the most overarching challenges related to faculty teaching through four emergent themes: pedagogical changes, work-life balance, face-to-face interactions, and physical and mental health. Five themes emerged that we used to describe the most overarching challenges related to students and their learning: learning patterns, technology access, additional responsibilities, learning community, and mental health. Based upon the identiﬁed challenges, we provide broad recommendations that can be used to foster a more successful transition to ERT in unforeseen regional or global crises in the future. M.K. ; B.C.C. , A.M., M.F. D. , T.D. B. M.K. ; supervision, T.D. B. ; project administration, T.D. B. ; funding acquisition, T.D. B.
Mild hypoxia in vivo regulates cardioprotective SUR2A

High-altituderesidentshavelowermortalityratesforischaemicheartdiseaseandthisisascribedtocardiacgene remodelling by chronic hypoxia. SUR2A is a cardioprotective ABC protein serving as a subunit of sarcolemmal ATP-sensitive K + channels. The purpose of this study was to determine whether SUR2A is regulated by mild hypoxia in vivo and to elucidate the underlying mechanism. Mice were exposed to either 21% (control) or 18% (mild hypoxia) oxygen for 24 h. Exposure to 18% oxygen did not affect partial pressure of O 2 (PO 2 ) and CO 2 (PCO 2 ) in the blood, haematocrit or level of ATP in the heart. However, hypoxia increased myocardial lactate dehydrogenase (LDH) and lactate as well as NAD + without affecting total NAD. SUR2A levels were signi ﬁ cantly increased as well asmyocardial resistance to ischaemia – reperfusion. Exposure to 18% oxygen did not phosphor- ylate extracellular signal regulated kinases (ERK1/2) or AMP activated protein kinase (AMPK), but it phosphorylated protein kinase B (Akt). An inhibitor of phosphoinositide 3-kinases (PI3K), LY294002 (0.2 mg/mouse), abolished all observed effects of hypoxia. LDH inhibitors, gallo ﬂ avin (50 μ M) and sodium oxamate (80 mM) signi ﬁ cantly decreased levels of SUR2A in heart embryonic H9c2 cells, while inactive mutant LDH form, gly193-M-LDH increased cellular sensitivity towards stress induced by 2,4-dinitrophenol (10 mM). Treatment of H9c2 cells with sodium lactate (30 mM) increased intracellular lactate, but did not affect LDH activity or SUR2A levels. We conclude that PI3K/Akt signalling pathway and LDH play a crucial role in increase of cardiac SUR2A induced by in vivo exposure to 18% oxygen.
MOLECULAR DETECTION AND ANTIMICROBIAL ACTIVITY OF ENTEROCOCCUS FAECALIS AND ENTEROCOCCUS FAECIUM ISOLATED FROM URINARY TRACT INFECTION (UTI) PATIENTS, ANIMALS AND POULTRY

Microbiological and polymerase chain reaction (PCR) methods was used to detect the occurrence of Enterococcus faecalis and Enterococcus faecium among 95 urine samples collected from patients suffering from urinary tract infection (UTI) recurrence admitted to outpatient clinics of private and governmental hospitals in Sohag city and 102 fecal and cloacal swabs collected from reared animals and / or poultry in some patient´s households. The results revealed that E. faecalis and E. faecium was detected in 13 (13.7%) patients, among them 11 patients were reared animals and / or poultry in their households, also isolated from 15 (14.7%) out of 102 fecal and cloacal samples of animals and poultry reared in 11 patient´s households. Referring to antimicrobial resistance and presence of esp gene among all enterococcal strains, E. faecalis and E. faecium isolates of both human and animals possess resistance to some antimicrobials with clinically importance for human therapy and esp gene was detected in 11 (84.65) out of 13 E. faecalis and E. faecium strains isolated from UTI patients and in 9 (60%) out of 15 E. faecalis and E. faecium strains isolated from the reared animals and poultry. This study suggests that reared animals and poultry, miss use of antimicrobials and presence of esp gene considered a risk factors for UTI recurrence caused by enterococci in human.
Interference pattern and visibility of a Mott insulator (6 pages)

We analyze theoretically the experiment reported in [F. Gerbier et al., Phys. Rev. Lett. 95, 050404 (2005)]. There, the interference pattern produced by an expanding atomic cloud in the Mott insulator regime was observed, indicative of short-range coherence in the system. The latter was traced back to the presence of a small amount of particle-hole pairs in the insulating phase for finite lattice depths. We analyze the influence of these pairs on the interference pattern using a random phase approximation, and derive the corresponding visibility. We also account for the inhomogeneity inherent to atom traps in a local density approximation. The calculations reproduce the experimental observations, except for very large lattice depths. The deviation from the measurement in this range is attributed to the increasing importance of nonadiabatic effects.
Dynamic Coupled Analysis of FSRU with Broken Mooring Line

In 2008~2013, KRISO (Korea Research Institute of Ships & Ocean Engineering) developed technologies for FSRU (Floating Storage Regasification Unit) engineering. Time domain dynamic coupled solver for floating body and mooring lines is one of the key results of the FSRU project. By applying the solver, this paper investigates dynamic behavior of FSRU system with broken mooring line. The size of analyzed FSRU is 315 m×53 m and it has nine catenary mooring lines. Higher order boundary element method (HOBEM) & convolution and finite element method (FEM) were applied to solve floating body and mooring line, respectively. Coupled solution is iteratively obtained by finding compatibility of fairleads. Irregular waves at mild and severe seas are considered as environmental loads. From the numerical analysis, this paper investigates how the body motions and line tensions change in the event of line breaking. All the results are compared with the case of intact mooring lines.
OP-JUEL210027 1..9

Urbanization is one of the major causes of biodiversity loss worldwide. Some species are able to adapt to urbanization, whereas others perish. Studies on long-term effects of the impact of urbanization on species diversity and abundance patterns are especially lacking from tropical cities. We seek to assess the relationship between urbanization and species richness of lake-dependent birds in Bangalore, a tropical mega-city in Southern India. We specifically ask: (i) How is bird species richness related to the size of the lake? (ii) How is bird species richness in Bangalore’s lakes related to the degree of urbanization? We used data from 2014 to 2019, collected from eBird—an online database that collates information on bird observations globally. Both lake area and distance from the city centre are correlated to species richness, with larger lakes supporting more bird species. As distance from the city centre increased (i.e. urbanization decreases), bird richness increased. Overall, in the city of Bangalore, migratory birds have declined while many lake-dependent resident birds seem to be increasing over the past 5 years. We hypothesize that birds that roost and nest in trees appear to be increasing. To confirm this, further research taking a trait-based approach is required. Urbanization appears to have species-specific impacts on lake-dependent birds in this tropical city, with certain groups of birds faring better than others. This research adds to the significant paucity of studies of the impact of urbanization on biodiversity in the urban tropics.
Social network dynamics and HIV transmission and S.

Objective: To prospectively study changes in the social networks of persons at presumably high risk for HIV in a community with low prevalence and little endogenous transmission. Methods: From a cohort of 595 persons at high risk (prostitutes, injecting drug users, and sexual partners of these persons) and nearly 6000 identified contacts, we examined the social networks of a subset of 96 persons who were interviewed once per year for 3 years. We assessed their network configuration, network stability, and changes in risk configuration and risk behavior using epidemiologic and social network analysis, and visualization techniques. Results: Some significant decrease in personal risk-taking was documented during the course of the study, particularly with regard to needle-sharing. The size and number of connected components (groups that are completely connected) declined. Microstructures (small subgroups of persons that interact intensely) were either not present, or declined appreciably during the period of observation. Conclusions: In this area of low prevalence, the lack of endogenous transmission of HIV may be related in part to the lack of a network structure that fosters active propagation, despite the continued presence of risky behaviors. Although the relative contribution of network structure and personal behavior cannot be ascertained from these data, the study suggests an important role for network configuration in the transmission dynamics of HIV.
Predictive factors for verbal memory performance over decades of ageing: Data from the Women’s

Background: Abnormalities in brain structure and function can occur several decades prior to the onset of cognitive decline. It is in the preceding decades that an intervention is most likely to be effective, when informed by an understanding of factors contributing to the disease prodrome. However, few studies have sufficient longitudinal data on relevant risks to determine the optimum targets for interventions to improve cognition in ageing. In this paper we examine the timing and exposure of factors contributing to verbal memory performance in later life. Methods: 387 participants from the population-based Women’s Healthy Ageing Project, mean age at baseline of 49.6 years (45 – 55), had complete neuropsychiatric assessments, clinical information, physical measures and biomarkers collected at baseline, with at least three follow-up visits that included at least one cognitive reassessment. Mixed Linear models were conducted to assess the significance of risk factors on later life verbal memory. We explored the influence of early, contemporaneous and cumulative exposures. Results: Younger age and better education were associated with baseline memory test performance. Over the 20 years of study follow-up, cumulative mid to late life physical activity had the strongest effect on better later life verbal memory (0.136 [.058, .214]). The next most likely contributors to verbal memory in late life were the negative effect of cumulative hypertension (-0.033 [-.047, -.0.18] and the beneficial effect of HDL cholesterol (0.818 [0.042, 1.593]). Conclusion: Findings suggest that midlife interventions focused on physical activity, hypertension control, and achieving optimal levels of HDL cholesterol will help maintain later-life verbal memory skills.