Miracle-dz/UniFAR-FTUs huggingface dataset:
Covid-19: EU states report 60% rise in emergency calls about domestic violence

A 60% increase in emergency calls from women subjected to violence by their intimate partner has been reported in the World Health Organization Europe member states. Comparing April 2020 with the same period last year, WHO said that online inquiries to violence prevention support hotlines had also increased as much as fivefold. This comes as many countries are in lockdown or are restricting people’s ability to leave their homes because of the covid-19 pandemic. The United Nations Population Fund has warned that continuing lockdowns for six months could result in an extra 31 million cases of gender based violence globally. It added that the pandemic was expected to “cause significant delays in programmes to end female …
Is It or Isn't It: The Importance of Visual Classification in Microplastic Characterization

Microplastics are a diverse category of pollutants, comprising a range of constituent polymers modified by varying quantities of additives and sorbed pollutants, and exhibiting a range of morphologies, sizes, and visual properties. This diversity, as well as their microscopic size range, presents numerous barriers to identification and enumeration. These issues are addressed with the application of physical and chemical analytical procedures; however, these present new problems associated with researcher training, facility availability and cost, especially for large-scale monitoring programs. Perhaps more importantly, the classifications and nomenclature used by individual researchers to describe microplastics remains inconsistent. In addition to reducing comparability between studies, this limits the conclusions that may be drawn regarding plastic sources and potential environmental impacts. Additionally, where particle morphology data is presented, it is often separate from information on polymer distribution. In establishing a more rigorous and standardized visual identification procedure, it is possible to improve the targeting of complex analytical techniques and improve the standards by which we monitor and record microplastic contamination. Here we present a simple and effective protocol to enable consistent visual processing of samples with an aim to contribute to a higher degree of standardization within the microplastic scientific community. This protocol will not eliminate the need for non-subjective methods to verify plastic objects, but it will standardize the criteria by which suspected plastic items are identified and reduce the costs associated with further analysis.
Microbial genomics amidst the Arctic crisis

The Arctic is warming – fast. Microbes in the Arctic play pivotal roles in feedbacks that magnify the impacts of Arctic change. Understanding the genome evolution, diversity and dynamics of Arctic microbes can provide insights relevant for both fundamental microbiology and interdisciplinary Arctic science. Within this synthesis, we highlight four key areas where genomic insights to the microbial dimensions of Arctic change are urgently required: the changing Arctic Ocean, greenhouse gas release from the thawing permafrost, 'biological darkening' of glacial surfaces, and human activities within the Arctic. Furthermore, we identify four principal challenges that provide opportunities for timely innovation in Arctic microbial genomics. These range from insufficient genomic data to develop unifying concepts or model organisms for Arctic microbiology to challenges in gaining authentic insights to the structure and function of low-biomass microbiota and integration of data on the causes and consequences of microbial feedbacks across scales. We contend that our insights to date on the genomics of Arctic microbes are limited in these key areas, and we identify priorities and new ways of working to help ensure microbial genomics is in the vanguard of the scientific response to the Arctic crisis.
Classification and assessment of degradation in European running waters

A pan-European, classification of the extent of environmental degradation from chemical, physical and biological pressures on fish communities as a precursor to assess the ecological status of running waters based on fish is proposed. Twenty-four potential pressures acting on fish communities at three different spatial scales (river basin, segment and site) were identified and class boundaries for high, good, moderate, poor and bad status, based on existing data and/or expert judgement, were defined. Four pressures (hydrological regime, morphological conditions, toxic or acid conditions, nutrients and organic load) were found to describe the majority of degradation at a specific site and these were combined into a single pressure variable to describe impact at each location. Principal Component Analysis showed that the four variables were correlated with other physical and chemical variables not included in the combined pressure variable. However, biological pressures, e.g. introduction of fish, and longitudinal connectivity were not well correlated, suggesting that two dimensions of human impact on stream fish were poorly accounted for. Low-resolution Geographical Information Systems (GIS) data (1 km grid) on land use and population density correlated well with the four chosen pressures, suggesting it is possible to use standardised GIS data to aid pre-classification of stream degradation.
Time Course of COVID-19 Cases in Austria

COVID-19 is an infectious disease caused by a novel coronavirus, which first appeared in China in late 2019, and reached pandemic distribution in early 2020. The first major outbreak in Europe occurred in Northern Italy where it spread to neighboring countries, notably to Austria, where skiing resorts served as a main transmission hub. Soon, the Austrian government introduced strict measures to curb the spread of the virus. Using publicly available data, we assessed the efficiency of the governmental measures. We assumed an average incubation period of one week and an average duration of infectivity of 10 days. One week after the introduction of strict measures, the increase in daily new cases was reversed, and the reproduction number dropped. The crude estimates tended to overestimate the reproduction rate in the early phase. Publicly available data provide a first estimate about the effectiveness of public health measures. However, more data are needed for an unbiased assessment.
Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation

The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr. ) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodulation and N2-fixing efficiency by producing larger nodules. To understand the role of S141 on soybean and USDA110 symbiosis, putative genes related to IAA biosynthesis were disrupted, suggesting that co-inoculation of USDA110 with S141ΔyhcX reduces the number of large size nodules. It was revealed that yhcX may play a major role in IAA biosynthesis in S141 as well as provide a major impact on soybean growth promotion. The disruption of genes related to cytokinin biosynthesis and co-inoculation of USDA110 with S141ΔIPI reduced the number of very large size nodules, and it appears that IPI might play an important role in nodule size of soybean–Bradyrhizobium symbiosis. However, it was possible that not only IAA and cytokinin but also some other substances secreted from S141 facilitate Bradyrhizobium to trigger bigger nodule formation, resulting in enhanced N2-fixation. Therefore, the ability of S141 with Bradyrhizobium co-inoculation to enhance soybean N2-fixation strategy could be further developed for supreme soybean inoculants.
Adipose‐resident myeloid‐derived suppressor cells modulate immune cell homeostasis in healthy mice

The metabolically dynamic nature of healthy adipose places this tissue under regular inflammatory stress. A network of adipose‐resident anti‐inflammatory immune cells modulates and resolves this endogenous inflammation. Previous work in our laboratory identified a CD11b+Gr1+ subset of these immunosuppressive adipose stromal cells in healthy mice. Myeloid‐derived suppressor cells (MDSCs), typically associated with cancer and chronic inflammation, have a similar surface marker phenotype and accumulate in adipose of high‐fat diet‐fed mice. Given the routine inflammatory stresses on healthy adipose and the suppressive nature of the tissue‐resident immune cells, we hypothesized that these CD11b+Gr1+ cells were a genuine population of MDSCs involved in regulating tissue homeostasis. Flow cytometric analysis of these cells found that they were CD11b+CD301− Ly6C+Ly6G+/− and did not express traditional macrophage markers. Moreover, in vitro functional assays demonstrated that these cells suppressed αCD3/αCD28‐induced T‐cell proliferation, solidifying their identity as bona fide adipose‐resident MDSCs. Systemic MDSC depletion altered adipose immune cell dynamics in otherwise healthy mice, increasing the number of CD4+ effector memory T cells and modifying the surface markers expressed by adipose‐resident macrophages. In addition, transcription of various immunomodulatory cytokines was clearly dysregulated in the adipose of MDSC‐depleted animals compared with controls. Altogether, our findings indicate that there is a population of bona fide MDSCs in the adipose of otherwise healthy mice that actively contribute to the health and immune homeostasis of this tissue.
A two-week summer program promoting physical activity: quality of life assessment in Italian children

ABSTRACT Physical activity (PA) can be associated with better health-related quality of life (HRQoL). This study aimed to assess HRQoL before and after a two-week summer program promoting PA in Italian school-aged children. Participants were recruited during the Giocampus summer2017 (Parma, Italy), from June to July. Before (T0) and after (T1) the program, children and one of their parents answered the Kindl questionnaire. For each domain, least-square mean changes (LSmc) at T1 were derived from linear regression models stratified by responder and adjusted for child gender, age group, time spent in PAs and HRQoL score of the responder at T0. 350 children (7-13 years, 52% males) and 342 parents answered the questionnaire at both T0 and T1. At T1, the HRQoL score of the children significantly improved in the emotional (LSmc 2.9, p<0.001), self-esteem (LSmc 3.3, p<0.001), family (LSmc 4.2, p<0.001) and friend (LSmc 3.1, p<0.001) domains. Parents reported significantly more improvement in self-esteem than children (LSmc 6.7 vs 3.3, p=0.012). Children spending more time in PA reported significantly more improvement in self-esteem than those doing less PA (LSmc 4.4 [p<0.001] vs 2.2 [p=0.181]). A short summer program promoting PA may improve HRQoL in the general population of school-aged children.
Oral administration with a traditional fermented multi-fruit beverage modulates non-specific and antigen-specific immune responses in BALB/c mice

Fruits have been widely considered as the default “health foods” because they contain numerous vitamins and minerals needed to sustain human health. Fermentation strategies have been utilized to enhance the nutritive and flavor features of healthy and readily consumable fruit products while extending their shelf lives. A traditional fermented multi-fruit beverage was made from five fruits including kiwi, guava, papaya, pineapple, and grape fermented by Saccharomyces cerevisiae along with lactic acid bacteria and acetic acid bacteria. The immunomodulatory properties of the fermented multi-fruit beverage, in vivo nonspecific and ovalbumin (OVA)-specific immune response experiments using female BALB/c mice were performed. Administration of the fermented multi-fruit beverage reduced the calorie intake, thus resulting in a less weight gain in mice compared to the water (placebo)-fed mice. In the nonspecific immune study model, the fermented multi-fruit beverage enhanced phagocytosis and T cell proliferation but did not affect B cell proliferation and immunoglobulin G (IgG) production. Analysis of cytokine secretion profile also revealed that the fermented multi-fruit beverage enhanced proinflammatory cytokines interleukin (IL)-6, tumor necrosis factor (TNF)-α, and T helper (Th)1-related cytokine interferon (IFN)-γ production, thus creating an immunostimulatory effect. Nonetheless, in the specific immune study model, the results showed that the fermented multi-fruit beverage decreased the production of proinflammatory cytokines IL-6 and TNF-α production in OVA-immunized mice. Moreover, it also caused a decrease in the production of anti-OVA IgG1, which was accompanied by a decrease in Th2-related cytokines IL-4 and IL-5 production and an increase in Th1-related cytokine IFN-γ production, indicating that it may have the potential to shift the immune system from the allergen‐specific Th2 responses toward Th1-type responses. The results indicate that fermented multi-fruit beverage has the potential to modulate immune responses both in a nonspecific and specific manners.
Verticillium longisporum and V. dahliae: infection and disease in Brassica napus

Verticillium wilt of oilseed rape (Brassica napus) is caused primarily by Verticillium longisporum and has become a serious problem in northern Europe. In order to evaluate whether V. longisporum and V. dahliae differ in their interaction with oilseed rape, phenotypical and molecular assessments were made. Oilseed rape plants for fungal assessments were inoculated with V. longisporum and V. dahliae via root-dipping and samples were taken from roots, stems, leaves, flowers, pods and seeds during plant development. The infection by V. longisporum was found to start mainly in lateral roots and root-hairs, followed by colonization of the xylem vessels and extensive spread in stems and leaves, whereas V. dahliae infected the main roots and remained in the region below the cotyledon node of the plants. Re-isolation studies, together with PCR analysis of samples taken from early growth stages through to fully ripe plants, showed that the onset of flowering was a critical phase for V. longisporum to colonize plants. No seeds infected with V. longisporum were found. Mycelial growth from V. dahliae but not V. longisporum was significantly reduced on media containing tissue from a low glucosinolate B. napus genotype compared with growth on media containing tissue from a high glucosinolate cultivar. The results of this study suggest that V. longisporum favours B. napus as host and that the transition from the vegetative to the generative phase is of importance for the spread of the fungus in oilseed rape plants.
Robust Asymptotic Fault Estimation of Discrete-Time Interconnected Systems With Sensor Faults

In this article, a robust asymptotic fault estimation (RAFE) design is proposed for discrete-time interconnected systems with sensor faults. By constructing a singular augmented system, an equivalent description of the considered interconnected systems is presented. Then, a novel RAFE observer is proposed for the singular augmented system. Furthermore, gain matrices of the RAFE observer are calculated based on multiconstrained design. Simulation results are illustrated to show the feasibility of the presented approaches.
Dinoflagellate cysts from Recent Danish marine sediments

Twenty-three different cyst types were found in a survey of dinoflagellate resting stages (cysts) in sediment samples from two sites in Danish waters: Oresund (The Sound) and Aarhus Bay. This is the first survey of its kind from Danish waters. The cyst types found were: Diplopsalis lenticula, Gymnodinium catenatum, Polykrikos schwartzii, Scrippsiella trochoidea, four species of Gonyaulax, ten species of Protoperidinium and five unidentified cyst types. Five species have not previously been reported from Danish waters. Where possible, the cysts were germinated and identification was based on characteristics of both cyst and motile stages. Among the germinated cysts was Gymnodinium catenatum, a naked dinoflagellate causing Paralytic Shellfish Poisoning (PSP) elsewhere in the world and not previously found living in Danish waters.
COVID-19: animals, veterinary and zoonotic links

Abstract Coronavirus disease 2019 (COVID-19), has spread over 210 countries and territories beyond China shortly. On February 29, 2020, the World Health Organization (WHO) denoted it in a high-risk category, and on March 11, 2020, this virus was designated pandemic, after its declaration being a Public Health International Emergency on January 30, 2020. World over high efforts are being made to counter and contain this virus. The COVID-19 outbreak once again proves the potential of the animal-human interface to act as the primary source of emerging zoonotic diseases. Even though the circumstantial evidence suggests the possibility of an initial zoonotic emergence, it is too early to confirm the role of intermediate hosts such as snakes, pangolins, turtles, and other wild animals in the origin of SARS-CoV-2, in addition to bats, the natural hosts of multiple coronaviruses such as SARS-CoV and MERS-CoV. The lessons learned from past episodes of MERS-CoV and SARS-CoV are being exploited to retort this virus. Best efforts are being taken up by worldwide nations to implement effective diagnosis, strict vigilance, heightened surveillance, and monitoring, along with adopting appropriate preventive and control strategies. Identifying the possible zoonotic emergence and the exact mechanism responsible for its initial transmission will help us to design and implement appropriate preventive barriers against the further transmission of SARS-CoV-2. This review discusses in brief about the COVID-19/SARS-CoV-2 with a particular focus on the role of animals, the veterinary and associated zoonotic links along with prevention and control strategies based on One-health approaches.
Risk Factors for Lower Extremity Amputation Following Attempted Free Flap Limb Salvage

Abstract Background Traumatic limb salvage with free flap reconstruction versus primary amputation for lower extremity (LE) injuries remains an oft debated topic. Limb salvage has well-studied benefits and advances in microsurgery have helped reduce the complication rates. A subset of patients eventually requires secondary amputation after a failed attempt at limb salvage. A better understanding of risk factors that predict subsequent amputation after failed free flap reconstruction of LE injuries may improve operative management. Patients and Methods A retrospective study (2002–2019) was conducted on all patients who underwent free flap reconstruction of the LE within 120 days of the original inciting event at a single institution. Patient and operative factors were reviewed including comorbidities, severity of the injury, flap choice, outcomes, and complications. Predictors of subsequent amputation were analyzed. Results A total of 129 patients requiring free flap reconstructions for LE limb salvage met inclusion criteria. Anterolateral thigh flaps (70.5%) were performed most frequently. Secondary amputation occurred in 10 (7.8%) patients. Preoperative factors associated with eventual amputation include diabetes mellitus (p = 0.044), number of preoperative debridements (p = 0.013), evidence of any arterial injury/pathology (p = 0.008), specifically posterior tibial artery (p = < 0.0001), and degree of three-vessel runoff (p = 0.007). Operative factors associated with subsequent amputation include evidence of recipient artery injury/pathology (p = 0.008). Postoperative factors associated with secondary amputation include total flap failure (p = 0.001), partial flap failure (p = 0.002), minor complications (p = 0.037), and residual osteomyelitis (p = 0.028). Conclusion Many factors contribute to the reconstructive surgical team's decision to proceed with limb salvage or perform primary amputation. Several variables are associated with failed limb salvage resulting in secondary amputation. Further studies are required to better guide management during the limb salvage process.
Intravenous Infusion of Cortisol, Adrenaline, or Noradrenaline Alters Porcine Immune Cell Numbers and Promotes Innate over Adaptive Immune Functionality

Key Points Cortisol strongly decreases porcine adaptive immune cells and increases neutrophils. Catecholamines exert acute effects on porcine immune cell numbers and function. All stress hormones promote innate over adaptive immune functionality in pigs. Despite the importance of pigs (Sus scrofa domestica) in livestock production and their increasing role as a model organism for human physiology, knowledge about the porcine immune system under the influence of stress hormones is fragmentary. Exceptionally little is known about the effects of catecholamines. Therefore, the aim of this study was to examine the in vivo effects of adrenaline, noradrenaline, and cortisol on number and functionality of porcine blood immune cells. Castrated male pigs (n = 34) were treated with physiological doses of either adrenaline, noradrenaline, or cortisol via i.v. infusion for 48 h. Blood samples were collected before treatment (−24 h, −22 h, 0 h), during treatment (+2 h, +24 h, +48 h), and at 72 h postinfusion. Immune cell numbers and phagocytic activity were evaluated by flow cytometry and lymphocyte proliferation by 3H-thymidine incorporation. Total IgG and IgM Ab levels were determined via ELISA. Pigs receiving cortisol showed strongly decreased adaptive immune cell numbers and increased neutrophils, accompanied by hampered lymphocyte proliferation but increased monocyte phagocytosis. Catecholamine effects on immune cell numbers were mostly similar to cortisol in direction but smaller in intensity and duration. Lymphocyte proliferation was inhibited after 2 h of noradrenaline infusion, and both catecholamines promoted monocyte and neutrophil phagocytosis. These findings indicate a shift from adaptive to innate immunity in stressful situations. This study is the first (to our knowledge) to systematically investigate specific glucocorticoid and catecholamine actions on the porcine immune system in this level of detail and confirms many similarities to humans, thus strengthening the pig as a human model in psychoneuroimmunology.
Tests of artificial substrata as nursery habitat for young fish

Recruitment areas for freshwater fish are often negatively affected by eutrophication and physical disturbances. Vegetated areas, which are important nursery habitats, are reduced and water turbidity increased. As a method of compensation, we tested artificial substrata for young-of-the-year fish. The structures were made of spruce bundles, with and without surrounding nets, and placed in a hyper-eutrophic very turbid environment and in an undisturbed area with clear water. Both habitats were devoid of submerged vegetation. Young fish abundance in treated areas was compared with adjacent reference sites. In the clear water area, the abundance of all investigated species – perch, pike, bream, silver-bream and roach – was higher in areas with artificial refuges. A similar response was evident for cyprinids in the turbid environment. High abundance of pikeperch and ruffe appeared in the hyper-eutrophic test area. Neither of these species, nor perch, was attracted to the artificial refuges. The lack of response in perch and pikeperch suggests that the importance of structural refuge decreases in very turbid water for these species. Of the two methods tested, spruce bundles with surrounding nets generally attracted most young fish, implying that the nets further increased the refuge capacity by reducing predation risk. The conclusion is that artificial habitats could improve recruitment habitats and that protective devices can increase refuge capacity.