Miracle-dz/UniFAR-FTUs huggingface dataset:
Early COVID-19 pandemic’s toll on tuberculosis services, WHO European Region, January to June 2020

Background Essential health services, including for tuberculosis (TB), are being affected by public health and social measures (PHSM) introduced to control COVID-19. In many settings, TB resources, facilities and equipment are being redirected towards COVID-19 response. Aim We sought to assess the COVID-19 pandemic’s impact on TB services in the World Health Organization (WHO) European Region. Methods The fifty-three European Region Member States were asked to report qualitative and quantitative data in quarter one and two (Q1 and Q2) 2020. TB notifications were triangulated with the severity score on domestic movement restrictions to assess how they may have influenced TB detection. Results Twenty-nine countries reported monthly TB notifications for the first half of 2019 and 2020. TB notifications decreased by 35.5% during Q2 2020 compared with Q2 2019, which is six-fold more than the average annual decrease of 5.1% documented during 2015–2019. The number of patients enrolled in rifampicin-resistant/multidrug-resistant TB treatment also decreased dramatically in Q2 2020, by 33.5%. The highest movement restriction severity score was observed between April and May 2020, which coincided with the highest observed decrease in TB notifications. Conclusion A decrease in TB detection and enrolment to treatment may cause increases in TB burden and threatens the Region’s ability to reach the TB targets of the 2030 Sustainable Development Goals, still this might be mitigated with rapid restoration of TB services and the implementation of targeted interventions during periods with severe PHSM in place, such as those introduced in response to the COVID-19 pandemic.
Biofilm inhibition and antifouling evaluation of sol-gel coated silicone implants with prolonged release of eugenol against Pseudomonas aeruginosa

Abstract The incidence of biofilm-linked catheter-associated urinary tract infections (CAUTIs) is increasing across the world. However, there is no clinical evidence to support the modifications of biomaterials, such as antimicrobial agent-coated catheters, that are known to reduce the risk of bacterial colonization and resistance development. The present study developed and tested silicone segments coated with an antivirulence agent, eugenol. The parameters for sol–gel preparation and coating were tailored to achieve a prolonged release of eugenol (for >35 days) at predefined antivirulence doses from dip-coated thin films. The eugenol-coated segments could prevent biofilm formation by Pseudomonas aeruginosa PAO1 as well as bacterial adhesion. Significant repression in the expression of virulence and biofilm-associated genes were recorded, confirming the antivirulence and biofilm inhibition properties of silicone segments coated with eugenol. The drug release profiles, efficacy analysis, neutrophil-response studies, and in vitro toxicity profiling further supported the contention that the activity of the eugenol-coated sections was effective and safe.
Internet Cognitive-Behavioral Therapy for Painful Chronic Pancreatitis: A Pilot Feasibility Randomized Controlled Trial

INTRODUCTION: Severe abdominal pain is a cardinal symptom of chronic pancreatitis (CP) associated with a high economic and societal burden. In other chronic pain conditions, cognitive-behavioral therapy (CBT) has demonstrated efficacy in improving patient outcomes (e.g., pain-related disability and depression). However, CBT has not yet been evaluated in adult patients with painful CP. We aimed to (i) evaluate the feasibility and acceptability of an adapted Internet CBT program for CP and (ii) generate pilot data regarding the effects of treatment on patient pain outcomes. METHODS: Thirty adults (mean age = 49.8 years, SD = 12.5; 80% women) with suspected or definite CP were randomized to Internet CBT (Pancreatitis Pain Course) versus control. The Pancreatitis Pain Course has 5 CBT lessons (e.g., thought challenging, relaxation, and activity pacing) delivered over 8 weeks. Pain interference, pain intensity, and quality of life were assessed at pretreatment, posttreatment, and the 3-month follow-up. Qualitative interviews were conducted at posttreatment with a subset of participants. RESULTS: Eighty percent of participants rated the program as highly acceptable; 64.3% completed all 5 lessons. Qualitative data revealed positive perceptions of program features, relevancy, and skills. Patients randomized to Internet CBT demonstrated moderate to large effects in reducing pain intensity and pain interference from baseline to 3 months. The proportion of treatment responders (>30% improvement) was significantly greater in the Internet-CBT group than in the control group (50% vs 13%, Fisher exact t test P = 0.04). DISCUSSION: In this first trial of CBT pain self-management in CP, feasibility, acceptability, and preliminary efficacy for reducing pain and disability were demonstrated. Future definitive trials of CBT are needed.
Comprehensive Cardiotoxicity Assessment of COVID-19 Treatments Using Human Induced Pluripotent Stem Cell-derived Cardiomyocytes.

Coronavirus disease 2019 (COVID-19) continues to spread across the globe, with numerous clinical trials underway seeking to develop and test effective COVID-19 therapies, including remdesivir. Several ongoing studies have reported hydroxychloroquine-induced cardiotoxicity, including development of torsade de pointes (TdP). Meanwhile, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are expected to serve as a tool for assessing drug-induced cardiotoxicity, such as TdP and contraction impairment. However, the cardiotoxicity of COVID-19 treatments has not been fully assessed using hiPSC-CMs. In the present study, we focused on drug repurposing with various modes of actions and examined the TdP risk associated with COVID-19 treatments using field potential using multi-electrode array (MEA) system and motion analysis with hiPSC-CMs. Hydroxychloroquine induced early after depolarization, while remdesivir, favipiravir, camostat and ivermectin had little effect on field potentials. We then analyzed electromechanical window (EMw), which is defined as the difference between field potential and contraction-relaxation durations. Hydroxychloroquine decreased EMw of hiPSC-CMs in a concentration-dependent manner. In contrast, other drugs have little effect. Our data suggest that hydroxychloroquine has proarrhythmic risk and other drugs have low proarrhythmic risk. Thus, hiPSC-CMs represent a useful tool for assessing the comprehensive cardiotoxicity caused by COVID-19 treatments in non-clinical settings.
Spatiotemporal Survey of Multiple Rice Diseases in Irrigated Areas Compared to Rainfed Lowlands in the Western Burkina Faso.

Multiple constraints affect rice yields and global production in West Africa. Among these constraints are viral, bacterial and fungal pathogens. We aimed to describe the spatiotemporal patterns of occurrence and incidence of multiple rice diseases in farmers' fields in contrasting rice growing systems in western Burkina Faso. For this purpose, we selected a set of three pairs of sites, each comprising an irrigated area and a neighboring rainfed lowland, and studied them over four consecutive years. We first performed interviews with the rice farmers to better characterize the management practices at the different sites. This study revealed that the transplanting of rice and the possibility of growing rice twice a year are restricted to irrigated areas, while other practices, such as the use of registered rice cultivars, fertilization and pesticides, are not specific but differ between the two rice growing systems. Then, we performed symptom observations at these study sites to monitor the following four diseases: yellow mottle disease, Bacterial Leaf Streak (BLS), rice leaf blast and brown spot. The infection rates were found to be higher in irrigated areas than in rainfed lowlands, both when analyzing all observed symptoms together (any of the four diseases) and when specifically considering each of the two diseases: BLS and rice leaf blast. Brown spot was particularly prevalent in all six study sites, while yellow mottle disease was particularly structured geographically. Various diseases were frequently found together in the same field (co-occurrence) or even on the same plant (coinfection), especially in irrigated areas.
Chemical and genetic rescue of in vivo progranulin-deficient lysosomal and autophagic defects

Significance Uncovering the genetic and cellular mechanisms of frontotemporal dementia (FTD) may lead to new therapeutic strategies. Mutations in the GRN gene are linked to FTD; the underlying cellular mechanisms are still unknown, but current research points to lysosomal dysfunction. Using the model organism Caenorhabditis elegans, we found a link between pgrn-1/GRN mutations, and sphingolipid metabolism and autophagy. We further identified small molecules that attenuated several pgrn-1/GRN phenotypes in vivo. These results offer sphingolipid metabolism as a potential mechanism contributing to FTD pathogenesis and suggest that efforts to restore sphingolipid homeostasis may be a beneficial therapeutic approach. In 2006, GRN mutations were first linked to frontotemporal dementia (FTD), the leading cause of non-Alzheimer dementias. While much research has been dedicated to understanding the genetic causes of the disease, our understanding of the mechanistic impacts of GRN deficiency has only recently begun to take shape. With no known cure or treatment available for GRN-related FTD, there is a growing need to rapidly advance genetic and/or small-molecule therapeutics for this disease. This issue is complicated by the fact that, while lysosomal dysfunction seems to be a key driver of pathology, the mechanisms linking a loss of GRN to a pathogenic state remain unclear. In our attempt to address these key issues, we have turned to the nematode, Caenorhabditis elegans, to model, study, and find potential therapies for GRN-deficient FTD. First, we show that the loss of the nematode GRN ortholog, pgrn-1, results in several behavioral and molecular defects, including lysosomal dysfunction and defects in autophagic flux. Our investigations implicate the sphingolipid metabolic pathway in the regulation of many of the in vivo defects associated with pgrn-1 loss. Finally, we utilized these nematodes as an in vivo tool for high-throughput drug screening and identified two small molecules with potential therapeutic applications against GRN/pgrn-1 deficiency. These compounds reverse the biochemical, cellular, and functional phenotypes of GRN deficiency. Together, our results open avenues for mechanistic and therapeutic research into the outcomes of GRN-related neurodegeneration, both genetic and molecular.
An initial preparation for human cadaveric dissection ameliorates the associated mental distress in students

It is universally recognized that cadaveric dissection is an essential part of anatomy training. However, it has been reported to induce mental distress in some students and impair their intrinsic motivation (IM) to study. One of the postulated reasons for this behavior is the lack of adequate information and preparation of students for cadaveric dissection. Therefore, it is hypothesized that providing relevant information prior to cadaveric dissection will ameliorate the mental distress, enhance the IM of students, and improve their academic performance. A cohort of occupational therapy students enrolled in an anatomy course were psychologically prepared for cadaveric dissection. Students were provided with a curated list of YouTube videos and peer‐reviewed journal articles related to cadaveric dissection prior to the commencement of the anatomy course. All students were also required to attend an oral presentation immediately before commencing dissection. The control group included students who had not been provided with any resources in preparation for cadaveric dissection. Compared to the control group, students who had been prepared demonstrated better quality of cadaveric dissection, improved academic performance, reported less mental distress and greater IM. Moreover, students reported the oral presentation to be most relevant and journal articles to be least useful in their preparation. Therefore, this is an effective approach in the amelioration of mental distress and improvement of performance in anatomy students. Consequently, this study represents a paradigm shift in the pedagogy of anatomy, and could represent a vital element in the evolution of a revitalized anatomy curriculum.
Comparison of the Clinical and Treatment Characteristics of Patients Undergoing Electroconvulsive Therapy for Catatonia Indication in the Context of Gender

The aim of this study was to compare in the context of gender both clinical diagnosis and disease-related differences and electroconvulsive therapy (ECT)-related differences in data and efficacy in hospitalized patients with catatonic signs and symptoms. Data from 106 patients who received ECT with catatonia indication were retrospectively analyzed. Clinical data of male (n = 58) and female (n = 48) patients were compared. Hospitalization documents and outpatient files, sociodemographic and clinical data form, Clinical Global Improvement scores used by the ECT unit in the follow-up of patients who received ECT were used in the study. It was seen that the mean age of women at the onset of ECT was higher than in men and the presence of prolonged seizures was more common than men. In men, it was found out that the average number of sessions with the onset of clinical response to treatment was higher than the average of women. The distribution of diagnoses by gender showed that the presence of schizophrenia diagnosis in men and of bipolar disorder in women were significantly more frequent compared to the opposite sex. It was found out that there were no significant differences between genders in terms of response rate to ECT. Our study is important for being the first study in the literature investigating the gender differences in ECT used for catatonia. However, gender is not a distinctive factor in the effectiveness of treatment, there are some important differences between male and female patients showing signs and symptoms of catatonia and undergoing ECT.
The role of colleague incivility in linking work-related stressors and job burnout. A cross-sectional study in a sample of faculty administrative employees

Background: Colleague incivility is one of the subtlest forms of workplace aggression, referring to any low-intensity deviant behavior in violation of the norms of mutual respect with ambiguous intent to harm the target. Although a large corpus of literature has identified the negative consequences of colleague incivility for workers and their organizations, there is a paucity of studies aimed at examining the role played by job characteristics in triggering this form of aggression. The present study, referring to the work environment assumption of Einarsen (2000), proposes that workplace aggression is primarily affected by factors related to deficiencies in the psychosocial work environment. In this view, the present study aimed to test whether the relationships between stressors in the psychosocial work environment (i.e., workload, role conflict, and unfair reward) and burnout (i.e., exhaustion and cynicism) are mediated by colleague incivility. Methods: The study design was cross-sectional and non-randomized. In total, 659 administrative officers employed in a large-sized Italian university completed a self-report questionnaire. Regression and mediation analyses (using the SPSS PROCESS macro) were performed to test the study hypotheses. Findings: After adjusting for control variables (i.e., superior incivility, age, gender, interactions with teaching staff, and interactions with students), the analyses indicated that colleague incivility mediated the associations of role conflict and unfair reward with the two dimensions of burnout. In contrast, the mediating role of colleague incivility in the associations of workload with exhaustion and cynicism was not supported. Discussion: The present study shed light on the key role of colleague incivility in the linkage of variables describing job characteristics and job burnout. From a practical point of view, the present study suggests that in order to prevent colleague incivility, interventions such as job (re)design should be implemented.
Salt Tolerance at Vegetative Stage is Partially Associated with Changes in Grain Quality and Starch Physicochemical Properties of Rice Exposed to Salinity Stress at Reproductive Stage.

BACKGROUND Rice yield and grain quality are highly sensitive to soil salinity. Distinct rice genotypes respond to salinity stress differently. To explore the variation in grain yield and grain trait adaptation to moderate, reproductive-stage salinity stress (4 dS/m electrical conductivity), four rice cultivars differing in degrees of vegetative salt tolerance, including Pokkali (salt-tolerant), RD15 (moderately salt-tolerant), KDML105 (moderately salt-susceptible) and IR29 (salt-susceptible), were examined. RESULTS Grain fertility and 100-grain weight of RD15, KDML105 and IR29, as well as grain morphology of KDML105 and IR29, were significantly disturbed. Interestingly, grain starch accumulation in RD15 and KDML105 was enhanced under stress. However, only RD15 showed changes in starch physicochemical properties, including increased granule diameter, decreased gelatinization peak temperature (Tp ) and decreased retrogradation onset temperature (To ). Notably, Pokkali maintained productivity, grain quality, and starch properties, while the grain quality of IR29 remained unchanged under salinity stress. Multivariate analysis displayed clear separation of productivity, grain morphology, and starch variables of RD15 in the salt-treated group relative to the control group, suggesting that it was the cultivar most impacted by salt stress despite its moderate salt-tolerance at vegetative stage. CONCLUSION Our results demonstrate specific salinity responses among the rice genotypes, and suggest discrepancies between degrees of salt tolerance at vegetative stage vs. the ability to maintain both grain quality and starch properties in response to salinity stress imposed at reproductive stage. This article is protected by copyright. All rights reserved.
Rtt105 promotes high-fidelity DNA replication and repair by regulating the single-stranded DNA-binding factor RPA

Significance Cells have evolved multiple mechanisms restraining the usage of lower-fidelity DNA repair pathways and polymerases. This work focuses on how deregulated Replication Protein A (RPA)–single-stranded DNA (ssDNA) assembly may impact the fidelity of genome duplication and repair. The absence of Rtt105 causes lower nuclear levels of RPA and impaired RPA-ssDNA assembly, resulting in increased replication errors with large deletions or duplications and elevated usage of the low-fidelity repair pathways. We show that this mechanism is likely conserved between yeast and humans. Thus, our work could provide insights into how human cells ensure high-fidelity DNA replication and repair. Furthermore, our study could potentially offer explanations for the genetic basis of some genome arrangements in human diseases. Single-stranded DNA (ssDNA) covered with the heterotrimeric Replication Protein A (RPA) complex is a central intermediate of DNA replication and repair. How RPA is regulated to ensure the fidelity of DNA replication and repair remains poorly understood. Yeast Rtt105 is an RPA-interacting protein required for RPA nuclear import and efficient ssDNA binding. Here, we describe an important role of Rtt105 in high-fidelity DNA replication and recombination and demonstrate that these functions of Rtt105 primarily depend on its regulation of RPA. The deletion of RTT105 causes elevated spontaneous DNA mutations with large duplications or deletions mediated by microhomologies. Rtt105 is recruited to DNA double-stranded break (DSB) ends where it promotes RPA assembly and homologous recombination repair by gene conversion or break-induced replication. In contrast, Rtt105 attenuates DSB repair by the mutagenic single-strand annealing or alternative end joining pathway. Thus, Rtt105-mediated regulation of RPA promotes high-fidelity replication and recombination while suppressing repair by deleterious pathways. Finally, we show that the human RPA-interacting protein hRIP-α, a putative functional homolog of Rtt105, also stimulates RPA assembly on ssDNA, suggesting the conservation of an Rtt105-mediated mechanism.
Qualitative differences in the mindsets associated with dual nature of normative commitment

This study aims to o uncover how employees’ normative commitment (sense of obligation) to their organization is experienced in terms of dual normative commitment (moral imperative or indebted obligation) and to describe the potential for different mindsets arising through the dynamic combination of the various components in the commitment profile. Semi-structured interviews were conducted with 16 participants. The interviews were designed to identify the respondents’ perceptions of obligation to their organisation, and their underlying motivational mindset associating with dual nature of normative commitment The interview findings for the affective-normative commitment dominant and the continuance commitment dominant participants were consistent with normative commitment experienced as either moral imperative or an indebted obligation, depending on the relative levels of affective and continuance commitment. All participants irrespective of their commitment profile noted that they had commitment to multiple foci, however, the alignment between commitment to these various foci differed by commitment profile. The qualitative differences among the commitment profiles indicated that the interaction of the commitment components is more complex than current commitment profile propositions suggest and that further theory development beyond the mindsets associated with continuance commitment and affective-normative commitment dominant profiles is required.
Histology of some major immune system organs in Lake Van fish Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)

The teleostean immune system is variable between and within taxa in terms of morphology. Accordingly, in the current study, the histology of some immune organs (thymus, kidney and spleen) of Lake Van fish (Alburnus tarichi) was investigated. For this purpose, the tissues from eight mature fish were fixed and embedded in paraffin wax, and then the sections were stained with haematoxylin and eosin, Mallory’s triple, periodic acid‐Schiff and Alcian blue (pH of 2.5) for histologic examinations. In the thymus, no clear discrimination of the cortex and medulla was observed. Lymphoid cells within a reticulo‐epithelial network were the predominant cell type in the thymus. The other components in the thymus were macrophages and myoid, mast‐like, plasma‐like, cystic cells, Hassall’s corpuscles, and single or multiple epithelial cystic structures. In the kidney, the head kidney, a major haematopoietic site, consisted of lymphoid and non‐lymphoid zones within a reticular network. Cells exhibiting mitotic figures were also detected in the haematopoietic tissue of the head kidney. Haematopoietic tissue was also found in the trunk kidney dispersed amongst the excretory components. The spleen was composed of red and white pulp. The red pulp comprised abundant erythrocytes, whilst the white pulp contained leucocytes with a reticular network. Ellipsoids were also determined in the white pulp. Melanomacrophage centres were found in all of the examined lymphoid tissues of the fish. These findings, which were reported, herein, for the first time will provide reference knowledge for future studies of this anadromous fish.