Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of combined treatment with pulsed electromagnetic field stimulation and sclerostin monoclonal antibody on changes in bone metabolism and pedicle screw augmentation in rabbits with ovariectomy-induced osteoporosis.

BACKGROUND Both pulsed electromagnetic field (PEMF) stimulation and sclerostin monoclonal antibody are useful for treating osteoporosis, but whether the two therapies have synergistic effects on both screw fixation quality and bone metabolism of ovariectomy-induced osteoporosis has not been reported. METHODS We used ovariectomy to create a rabbit model of postmenopausal osteoporosis. Then, specimens were fixed with pedicle screws in the L4 vertebral body. Rabbits were randomly divided into an OVX control group, PEMF group, Scl-Ab group, and PEMF+Scl-Ab group. The PEMF group was given PEMF magnetic therapy, the Scl-Ab group was administered a subcutaneous Scl-Ab injection, and the PEMF+SclAb group received both therapies. The OVX group was injected subcutaneously with the same dose of saline instead. After eight weeks of treatment, the bone metabolism index, bone mineral density (BMD), and bone microstructural, biological, and biomechanical parameters were evaluated. RESULTS BMD significantly decreased six months post-ovariectomy. Compared with that of the OVX group, the BMD of the PEMF, Scl-Ab, and PEMF+Scl-Ab groups increased by 20.3%, 19.9%, and 35.0%, respectively. The maximum pulling force of pedicle screws increased by 14.0%,15.0% and 19.1%, and the maximum failure power consumption of pedicle screws increased by 27.9%, 27.2% and 33.6%, respectively; these differences were statistically significant (P<0.05). The bone metabolism index and bone microstructure parameters of the PEMF+Scl-Ab group were more optimal than those in the single treatment group. CONCLUSIONS Both Scl-Ab and PEMF therapy can enhance the BMD and the mechanical strength of pedicle screws in osteoporotic bones of rabbits with postmenopausal osteoporosis. However, combination of the two measures has achieved even better results, yielding potential clinical application value.
Prevalence of extended high-frequency hearing loss among adolescents from two rural areas in Colombia

Abstract Objective Worldwide research trends on the prevalence of hearing loss within young rural populations are scarce. Current evidence suggests extended high-frequency audiometry could be a sensitive predictor to detect subclinical hearing loss. This study aimed to determine the prevalence of hearing loss through conventional pure tone (0.25–8 kHz) and extended high-frequency (9–20 kHz) audiometry tests in a group of adolescents aged 13 to 19 years old from two rural areas of Colombia. A sociodemographic and associated factor for hearing loss questionnaire was applied. Design Cross-sectional study. Study Sample Three hundred sixty-six adolescents aged 13 to 19 years old from two rural municipalities in Colombia: Soracá, and Palmas del Socorro. Results Hearing loss prevalence assessed through pure tone audiometry was 3.28% in right ears and 3.83% in left ears while through extended high-frequency audiometry was 14.21% and 13.11%, respectively. Associated factors found by the logistic regression were age (OR: 1.36; CI 95% 1.13-1.64) and obesity (OR: 3.74; CI 95% 1.07-13.11). Conclusions Using 15 dB as the normal hearing threshold parameter for audiometric frequencies, our results suggest that extended high-frequency hearing loss is already widespread among rural adolescents. This highlights the need for research on extended high-frequency audiometry in rural school-aged children.
Coronary Artery Bypass Grafting or Fractional Flow Reserve–Guided Percutaneous Coronary Intervention in Diabetic Patients With Multivessel Disease

Supplemental Digital Content is available in the text. Background: In diabetic patients with multivessel coronary artery disease, coronary artery bypass grafting (CABG) has shown long-term benefits over percutaneous coronary intervention (PCI). Physiology-guided PCI has shown to improve clinical outcomes in multivessel coronary artery disease, though its impact in diabetic patients has never been investigated. We evaluated long-term clinical outcomes of diabetic patients with multivessel coronary artery disease treated with fractional flow reserve (FFR)–guided PCI compared with CABG. Methods: From 2010 to 2018, 4622 diabetic patients undergoing coronary angiography were screened for inclusion. The inclusion criterion was the presence of at least 2-vessel disease defined as with diameter stenosis ≥50%, in which at least 1 intermediate stenosis (diameter stenosis, 30%–70%) was treated or deferred according to FFR. Inverse probability of treatment weighting analysis was used to account for baseline differences with a contemporary cohort of patients treated with CABG. The primary end point was major adverse cardiovascular and cerebrovascular events, defined as all-cause death, myocardial infarction, revascularization, or stroke. Results: A total of 418 patients were included in the analysis. Among them, 209 patients underwent CABG and 209 FFR-guided PCI. At 5 years, the incidence of major adverse cardiovascular and cerebrovascular events was higher in the FFR-guided PCI versus the CABG group (44.5% versus 31.9%; hazard ratio, 1.60 [95% CI, 1.15–2.22]; P=0.005). No difference was found in the composite of all-cause death, myocardial infarction, or stroke (28.8% versus 27.5%; hazard ratio, 1.05 [95% CI, 0.72–1.53]; P=0.81). Repeat revascularization was more frequent with FFR-guided PCI (24.9% versus 8.2%; hazard ratio, 3.51 [95% CI, 1.93–6.40]; P<0.001). Conclusions: In diabetic patients with multivessel coronary artery disease, CABG was associated with a lower rate of major adverse cardiovascular and cerebrovascular events compared with FFR-guided PCI, driven by a higher rate of repeat revascularization. At 5-year follow-up, no difference was observed in the composite of all-cause death, myocardial infarction, or stroke between CABG and FFR-guided PCI.
Impaired vasodilation in pregnant African Americans: Preliminary evidence of potential antecedents and consequences.

Significant health disparities exist between African Americans (AA) and European Americans (EA) in hypertension and hypertension-related disorders. Evidence suggests that this is due to impaired vasodilation in AAs. Pregnancy is a potent systemic vasodilatory state. However, differences in vasodilation between AAs and EAs have not been investigated in pregnancy. We sought to examine the effects of pregnancy on vasodilation in AA and EA women and how this might be related to discrimination and low birth weight in their offspring. Hemodynamics [blood pressure (MAP), cardiac output (CO), total peripheral resistance (TPR)] and heart rate variability (HF-HRV) were examined at baseline in 40 pregnant AAs (n = 20) and EAs (n = 20) and matched nonpregnant women (n = 40). The Experiences of Discrimination scale and birth weight were also measured in the offspring of the pregnant participants. Whereas pregnancy was associated with decreased MAP independent of race, AAs showed impaired vasodilation independent of pregnancy status as indicated by greater TPR despite greater HF-HRV. In AAs, but not EAs, reports of fewer incidences of discrimination were associated with greater TPR. Finally, the HF-HRV of EA mothers was inversely related to the birth weight of their offspring but was uncorrelated in AAs. We report novel evidence of impaired vasodilation to an endogenous vasodilatory stimulus in AAs. Higher TPR was related to discrimination in AAs and higher HF-HRV was related to low birth weight in EAs. These findings have implications for understanding the intergenerational transmission of impaired vasodilation in AAs.
Revisiting cultural competence

Although the importance of developing health care trainees and trainers’ cultural competence is widely acknowledged, limited information is available on what elements of cultural competence should be taught. A lack of theoretical clarity and consensus over what constitutes the competence attributes is a key hurdle in faculty development (FD). This results in patchy cultural education across health schools globally. Based on a literature review, we propose a new framework that may inform clinical cultural education and the development of clinical educators in this area.
Detection and Analysis of Plasmid-Mediated Quinolone Resistance Genes in Salmonella Isolates from Food Animals

To investigate the prevalence of plasmid-mediated quinolone resistance(PMQR) in Salmonella isolates from food animals,susceptibility of 316 Salmonella isolates from food animals to 20 antimicrobial agents were determined by the broth microdilution method,the prevalence of PMQR genes in the isolates were detected by PCR.The results showed that 316 Salmonella isolates exhibited different resistance to 20 antimicrobial agents,95.57% of isolates showed multi-resistance;The positive rates of qnrA,qnrC,qnrD,qnrS,qepA,qnrB,aac(6′)-Ib-cr,oqxA and oqxB genes were 0%,0%,0%,0%,0%,7.91%,15.19%,7.91% and 8.86%,respectively.oqxAB gene was the first time reported in Salmonella;98.11% of PMQR-positive strains,which showed multiple-resistance to 8-17 antimicrobials,harboured two or more resistant genes,qnrB aac(6′)-Ib-cr was the most common in these strains;The results of PFGE displayed 53 PMQR-positive strains belonged to 5 PFGE patterns.Some strains,which had different resistant phenotype or genotype,showed same PFGE patterns.In conclusion,316 Salmonella isolates tested showed serious resistance to antimicrobial agents;qnrB,aac(6′)-Ib-cr and oqxAB genes were frequence in strains;Clonal strain was epidemic in some resistant isolates from different origins.
Effect of Anti-hotstressors on Serum Biochemical Indexes and Immunity of Finishing Pigs

three way crossbred[Duroc×(Landrace×Yorkshire)]finishing pigs(60kg)ewre divided into 5 groups randomly and each group had two replicates with 5 pigs per replicate.Pigs of control group were fed basal diet and other groups were fed diets supplemented with 500mg/kg vitamin C,200mg/kg vitamin E,10mg/kg daidzein or 400mg/kg taurine respectively.The following were the results of the experiment lasting 39 days:The serum urea nitrogen concentration of each treatment group was not significantly different from that of control group (P0.05). The serum glucose concentration of each treatment guoup was lower than that of control group(P0.05). The concentration of HCO - 3,buffer base and base excess in serum of vitamin C or vitamin E or taurine was accordingly increased from the control group.The serum Cortisol concentration and the CD + 4/CD + 8 ratio in serum of each treatment group was lower than that of control group(P0.05). It was concluded that vitamin C and vitamin E and daidzein and taurine had better effect on the serum biochemical indexes and immunity of finishing pigs in a high temperature environment.
The current outcomes and future challenges in pediatric vascularized composite allotransplantation

Purpose of review We review the outcomes and future challenges associated with pediatric vascularized composite allotransplantation, including follow-up data from our bilateral pediatric hand–forearm transplantation. Recent findings In 2015, the first heterologous pediatric upper extremity hand–forearm transplant was performed at the Children's Hospital of Philadelphia, and in 2019, the first pediatric neck reconstructive transplantation was performed in Poland. The 5-year follow-up of the pediatric upper extremity recipient demonstrates similar growth rates bilaterally, an increase in bone age parallel to chronologic age, and perhaps similar overall growth to nontransplant norms. The pediatric upper extremity recipient continues to make gains in functional independence. He excels academically and participates in various extracurricular activities. Future challenges unique to the pediatric population include ethical issues of informed consent, psychosocial implications, limited donor pool, posttransplant compliance issues, and greater life expectancy and therefore time to inherit the many complications of immunosuppression. Summary Currently, we recommend pediatric vascularized composite allotransplantation (VCA) for bilateral upper extremity amputees, preferably on immunosuppression already, and those patients who would have the most potential gain not available through standard reconstructive techniques while being able to comply with postoperative immunosuppression protocols, surveillance, rehabilitation, and follow-up.
Validation and clinical evaluation of a SARS-CoV-2 surrogate virus neutralisation test (sVNT)

ABSTRACT To understand SARS-CoV-2 immunity after natural infection or vaccination, functional assays such as virus neutralising assays are needed. So far, assays to detect SARS-CoV-2 neutralising antibodies rely on cell-culture based infection assays either using wild type SARS-CoV-2 or pseudotyped viruses. Such assays are labour-intensive, require appropriate biosafety facilities and are difficult to standardize. Recently, a new surrogate virus neutralisation test (sVNT) was described that uses the principle of an ELISA to measure the neutralisation capacity of anti-SARS-CoV-2 antibodies directed against the receptor binding domain. Here, we performed an independent evaluation of the robustness, specificity and sensitivity on an extensive panel of sera from 269 PCR-confirmed COVID-19 cases and 259 unmatched samples collected before 2020 and compared it to cell-based neutralisation assays. We found a high specificity of 99.2 (95%CI: 96.9–99.9) and overall sensitivity of 80.3 (95%CI: 74.9–84.8) for the sVNT. Clinical sensitivity increased between early (<14 days post symptom onset or post diagnosis, dpos/dpd) and late sera (>14 dpos/dpd) from 75.0 (64.7–83.2) to 83.1 (76.5–88.1). Also, higher severity was associated with an increase in clinical sensitivity. Upon comparison with cell-based neutralisation assays we determined an analytical sensitivity of 74.3 (56.4–86.9) and 98.2 (89.4–99.9) for titres ≥10 to <40 and ≥40 to <160, respectively. Only samples with a titre ≥160 were always positive in the sVNT. In conclusion, the sVNT can be used as an additional assay to determine the immune status of COVID-19 infected of vaccinated individuals but its value needs to be assessed for each specific context.
Identification of Measures Affecting Job Satisfaction and Levels of Perceived Stress and Burnout among Home Health Nurses of a Developing Asian Country

Abstract Background Factors affecting job satisfaction of home health nurses are studied. Methods PSS-10, CBI and HHNJS are used to measure the perceived stress, burnout of home health nurses and Job Satisfaction. Results The significant predictors for Job satisfaction were Relationship with organization, Autonomy and control, Salary and Benefits as 82% of variation in job satisfaction was explained by these, (F change (3, 144) = 236.72, p < . 001). Conclusion Agencies should work upon improving the dimensions such as the “relationship with the organization”, “Autonomy and control” and “Salary and Benefits" to increases the Job satisfaction of home health nurses.
Facilitators and barriers to healthy food selection at children’s sports arenas in Norway: a qualitative study among club managers and parents

Abstract Objective: To investigate club managers’ and parents’ experiences with food selection at handball halls in order to identify facilitators and barriers to the availability of healthy food. Design: Individual interviews with club managers (n 6) and focus groups (n 5) with parents (n 21) were conducted. Interviews were recorded and transcribed verbatim, transcripts were coded in NVivo and the analysis was guided by thematic analysis. Setting: Interviews were conducted at five handball clubs with varying socio-economic user populations and sizes in the area of Oslo, Norway. Participants: The club managers were responsible for food selection at the handball clubs. The participating parents had one or two active children between the ages of 6 and 12 years who took part in the clubs. Results: The club managers and parents generally described food selection at the handball halls as unhealthy and wanted a healthier selection of food. The club managers’ primary barriers to providing a healthier food selection included the potential to lose profits, limited facilities and time to prepare these foods. The parents often valued unhealthy food, as they believed that it supported the social environment and served as a reward for the children. Trainers were perceived as important role models for the promotion of healthy eating. The participants thought that national guidelines could facilitate healthy food environments in sports arenas. Conclusion: Healthier food options in sports settings could be facilitated through national guidelines that describe healthy foods and establish who is responsible for providing healthy food selections.
TOXICITY OF INSECTICIDES COMMONLY USED IN VEGETABLE FIELDS TO THE DIAMONDBACK MOTH, PLUTELLA XYLOSTELLA, AND ITS PARASITE, COTESIA PLUTELLAE

Toxicity of 9 insecticides commonly used in vegetable fields to the larvae of the diamondback moth (DBM), Plutella xylostella (L.), and its major larval parasite, Cotesia plutellae Kurdjumov, was investigated in the laboratory. When tested at the rates recommended for field application by the producers, fipronil, Bt and avermectin produced 100% kill of the 3rd instar larvae of DBM, a mixture of Bt and virus, diafenthiuron and chlorfluazuron over 95% kill, and “shachongshuang” 80% kill. However, fenvalerate and methomyl produced rather low rates of kill, and over 70% of the DBM larvae tested could still pupate normally. When adult parasites were exposed to the fresh, dry insecticide film, “shachongshuang” killed more than 80% of the adults in 2 h, and fipronil 100% in 12 h. After 24 h exposure to the insecticide film, more than 90% of adults were killed by methomyl and “shachongshuang”, about 50% of the adults were killed by diafenthiuron and fenvalerate. The mortality caused by chlorfluazuron, Bt, avermectin and the mixture of Bt and virus were all below 20%, as that in the control. It is recommended that avermectin, Bt, the mixture of Bt and virus, diafenthiuron, and chlorfluazuron should be selected to control DBM during seasons when C.plutellae and other natural enemies are relatively abundant. Fipronil should be used only when DBM is very abundant and natural enemies are in very low numbers.
Spatial Reference Frame but Neither Age nor Gender Predict Performance on a Water-Level Task in 8- to 11-Year-Old Children

Successful performance on the water-level task, a common measure of spatial perception, requires adopting an environmental, rather than object-centered, spatial frame of reference. Use of this strategy has not been systematically studied in prepubertal children, a developmental period during which individual differences in spatial abilities start to emerge. In this study, children aged 8 to 11 reported their age and gender, completed a paper-and-pencil water-level task, and drew a map of their neighborhood to assess spontaneous choice of spatial frame of reference. Results showed a surprising lack of age or gender difference in water-level performance, but a significant effect of spatial frame of reference. Although they made up only a small portion of the sample, children who drew allocentric maps had the highest water-level score, with very high accuracy. These results suggest that children who adopt environmental-based reference frames when depicting their familiar environment may also use environmental-based reference frame strategies to solve spatial perception tasks, thereby facilitating highly accurate performance.
Identification of Hsa_circ_0104824 as a Potential Biomarkers for Breast Cancer

Background: To study the specific expression of circular RNA (circRNA) in breast cancer and adjacent normal tissues to identify differentially expressed circRNA in breast cancer patients. To study the correlation between circRNA and clinical data of breast cancer, and to evaluate its potential as a breast cancer biomarker. Methods: The differential expression of circRNAs between the breast cancer tissues and adjacent normal tissues was screened by Human CircRNA Microarray. Candidate circRNAs verified by qRT-PCR. CircRNA was analyzed by Agilent GeneSpring 13.0 software. SPSS23.0, GraphPad Prism, and Sigmaplot software were used for statistical analysis. Perform T test, one-way ANOVA, curve regression analysis and ROC curve analysis for evaluating the diagnostic value of circular RNA. Results: Among the 2021 differentially expressed circRNAs, 546 were up-regulated and 1475 were down-regulated in breast cancer tissues. The validation study demonstrated that six circRNAs were downregulated. Among them, hsa_circ_0104824 proved high correlation between breast cancer tissues and plasma samples in its expression and diagnostic value. Conclusion: Hsa_circ_0104824 may serve as a promising predictive biomarker and therapeutic target for patients with breast cancer.
Cerebral infarct site and affected vascular territory as factors in breathing weakness in patients with subacute stroke.

OBJECTIVE A better understanding of factors influencing breathing weakness in stroke survivors would help in planning rehabilitation therapies. The main objective of this study was to determine whether the location of cerebral infarct is associated with breathing weakness in patients with subacute stroke. DESIGN Cross-sectional analysis of a prospective cohort. PATIENTS Consecutive patients admitted to a neurology rehabilitation unit with first-time ischaemic stroke (n = 170). METHODS Breathing weakness was defined as > 70% reduction in maximal inspiratory and expiratory pressures (PImax and PEmax, respectively) compared with reference values. Computed tomography and magnetic resonance imaging were used to locate stroke lesions, which were classified as cortical, subcortical, cortico-subcortical, brainstem, or cerebellum. The affected cerebrovascular territory was identified to classify stroke subtype. The association between maximal respiratory pressure and affected brain area was studied using median regression analysis. RESULTS Breathing weakness was detected in 151 (88.8%) patients. Those with cortical and cortico-subcortical stroke location had the lowest PImax and PEmax values (median 33 cmH2O). This value differed significantly from maximal respiratory pressures of patients with strokes located in the brainstem and the cerebellum, with PImax median differences (β) of 16 cmH2O (95% confidence interval (95% CI) 4.1-27.9) and 27 cmH2O (95% CI 7.8-46.2), respectively, and PEmax median differences of 27 cmH2O (95% CI 11.4-42.7) and 49 cmH2O (95% CI 23.7-74.3), respectively, both of which remained significant after adjustments. CONCLUSION The prevalence of breathing weakness was very high in stroke patients admitted to a neurorehabilitation ward, being more severe in cortical or cortico-subcortical stroke.