Miracle-dz/UniFAR-FTUs huggingface dataset:
Morroniside Promotes PGC-1α-Mediated Cholesterol Efflux in Sodium Palmitate or High Glucose-Induced Mouse Renal Tubular Epithelial Cells

Lipid deposition is an etiology of renal damage caused by lipid metabolism disorder in diabetic nephropathy (DN). Thus, reducing lipid deposition is a feasible strategy for the treatment of DN. Morroniside (MOR), an iridoid glycoside isolated from the Chinese herb Cornus officinalis Sieb. et Zucc. , is considered to be an effective drug in inhibiting oxidative stress, reducing inflammatory response, and countering apoptosis. To explore the protective mechanism of MOR in attenuating renal lipotoxicity in DN, we investigated the effect of MOR on an in vitro model of lipid metabolism disorder of DN established by stimulating mouse renal tubular epithelial cells (mRTECs) with sodium palmitate (PA) or high glucose (HG). Oil Red O and filipin cholesterol staining assays were used to determine intracellular lipid accumulation status. Results revealed that PA or HG stimulation inhibited the expressions of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), liver X receptors (LXR), ATP-binding cassette subfamily A member 1 (ABCA1), ABCG1, and apolipoprotein E (ApoE) in mRTECs as evidenced by western blot and quantitative real-time PCR, resulting in increased intracellular lipid deposition. Interestingly, MOR upregulated expressions of PGC-1α, LXR, ABCA1, ABCG1, and ApoE, thus reducing cholesterol accumulation in mRTECs, suggesting that MOR might promote cholesterol efflux from mRTECs via the PGC-1α/LXR pathway. Of note, silencing PGC-1α reversed the promotive effect of MOR on PA- or HG-induced cellular cholesterol accumulation. In conclusion, our results suggest that MOR has a protective effect on mRTECs under high lipid or high glucose conditions, which may be related to the promotion of intracellular cholesterol efflux mediated by PGC-1α.
EDUCATIONAL VALUES IN NOVEL MIMPI ANAK PULAU BY ABIDAH EL KHALIEQY REVIEWED FROM INTRINSIC STRUCTURE STUDY

This research aims to explore and gain a deep understanding of the values of education in the novel Mimpi Anak Pulau by Abidah El Khalieqy in terms of the novel's intrinsic structure (theme, plot, characters, characterizations, and settings). This research is descriptive qualitative research. The method used is content analysis. Data collection techniques and procedures used research instruments in the form of notebooks, intrinsic element analysis sheets, analysis sheets of educational values in the novel Mimpi Anak Pulau by Abidah El Khalieqy. The results showed that the educational values in the form of religion/diligent worship, honesty, tolerance, discipline, hard work, creativity, independence, democracy, curiosity, love of the country, achievement, friendship, love of peace, love to read, concern for the environment, social care, and responsibility, are found and described in the novel Mimpi Anak Pulau by Abidah El Khalieqy. These educational values through the analysis of the intrinsic structure, which includes themes, characters and characterizations, plot, and setting.
Clinical and prognostic characteristics of 95 cases of Langerhans cell histiocytosis in children: a single-institute experience from 2013 to 2020

Abstract Background This study aimed to understand the clinical characteristics and outcomes of children with Langerhans cell histiocytosis (LCH) in China. Methods We conducted a retrospective study of 95 paediatric patients with LCH in West China Second University Hospital of Sichuan University between July 2013 and August 2020. Results The onset age of multisystem LCH (MS-LCH) patients with risk organ (RO) involvement was younger than that of MS-LCH without RO involvement (p = .002) and single system LCH (p < .001) patients; bone was the most frequently involved organ, followed by the skin. Of all, the BRAF-V600E mutation was detected in 48 out of 84 patients who underwent gene analysis. Additionally, in our study, BRAF p.N486_T491 > K, BRAF p.L485_P490delinsF, BRAF p.R506_K507insLLR, ARAF p.Q349_F351delinsL and MAP2K1 p.Q58_E62del were known mutations in the mitogen-activated protein kinase (MAPK) pathway. The BRAF-V600E genotype in the tissue and plasma prior to therapy were detected in 16 patients, and the concordance was only 37.5% (6/16). According to the modified LCH-III-based-protocol, JLSG-02 protocol chemotherapy, and vemurafenib, the estimated five-year overall survival, event-free survival (EFS) and cumulative reactivation rates of 95 patients were 98.8%, 74.6% and 24.5%, respectively. The EFS rate in good responders was better than that in poor responders at 12-week (HR = 0.022, 95%CI 0.002–0.231, p = .002), and EFS was not affected by age, RO involvement or BRAF-V600E mutation. Regarding sequelae, nine patients had central diabetes insipidus and two had growth retardation. Conclusions In this study, LCH was a highly heterogeneous disease characterized molecularly by MAPK-pathway activating mutations. Vincristine, prednisone and cytarabine-based chemotherapy combined with vemurafenib improved the prognosis of childhood LCH. In future, prospective clinical trials and novel therapeutic strategies should be developed to improve outcomes in paediatric patients with LCH. KEY MESSAGE Children with Langerhans cell histiocytosis in China present highly heterogeneous clinical characteristics, with up to 60% of cases harbouring mutations in MAPK pathway. Treatment response at 12-week is associated with EFS in our study. Vincristine, prednisone and cytarabine-based chemotherapy combined with vemurafenib improved the prognosis of Chinese childhood LCH, but the reactivation rate is still high.
The Effect Entrepreneurship Education and Subjective Norm on Biology Students’ Self-Efficacy in Entrepreneurial

This study aims to determine the effect of entrepreneurship education and subjective norms on students’ selfefficacy in the field of entrepreneurship. This study is an exploratory research with 35 students’ of biology education as research subjects. The research instrument used is a closed questionnaire with answers that are degraded according to the Likert scale which has been declared valid and reliable to use. Analysis of research data used is descriptive statistics and inferential statistics with multiple linear regression test at a significance level of 5%. The results of the study show that (1) students' perceptions of entrepreneurship education have an average of 3.15 in the Good category, subjective norms of 3.06 in the Good category, and self-efficacy of 3.12 in the Good category; (2) There is an effect of entrepreneurship education and subjective norms on students’ self-efficacy with an F value of 11.713 and a significance value (p) <0.05; (3) Entrepreneurship education and subjective norms simultaneously affect the self-efficacy of biology students’ by 42.3% and the rest are influenced by other variables or factors.
The Impact of the COVID-19 Pandemic on Future Nursing Career Turnover Intention Among Nursing Students

Introduction A shortage of nurses has been a major global concern, particularly during pandemics. Nursing students turning away from the nursing profession upon graduation may exacerbate nursing workforce shortfalls. The main objective of this study was to assess perceived occupational turnover intention among nursing students and associated factors (fear of COVID-19 and life satisfaction). Students were also asked to provide suggestions that could enhance their intention to join the nursing profession. Methods An online survey was sent to all registered undergraduate nursing students at Fujian Medical University, China. The partial least squares structural equation model (PLS-SEM) was used to investigate key factors influencing turnover intention. Results A total of 1020 complete responses were received (response rate: 86.2%). Nearly half (49.1%) reported that they would choose not to be on a nursing course if given a choice, 45.4% often think of not going into the nursing profession in the future, and 23.7% would consider entering a healthcare industry that has zero contact with patients. The total turnover intention score range was 3 to 15, and the mean ± standard deviation (SD) was 9.2 (SD ± 2.5). PLS-SEM path analysis revealed that fear of COVID-19 (β = 0.226, p < 0.001) had a positive effect on turnover intention. Satisfaction with life (β = −0.212, p < 0.001) had a negative effect on turnover intention. Analysis of open-ended survey data on students’ perspectives on how to encourage nursing students to enter the nursing workforce revealed five central themes: 1) professional role, respect, and recognition; 2) higher wages; 3) reduce workload; 4) enhance occupational health and safety; and 5) career advancement opportunities. Conclusion Factors influencing turnover intention and suggestions to reduce students’ apprehension towards joining the nursing profession found in this study should be seriously taken into consideration in initiatives to address the nurse shortages.
Effects of moisture and temperature on Salmonella survivability in beef tallow, white grease, and chicken rendered fat

Abstract High moisture levels introduced to fats after the rendering process can lead to Salmonella presence and growth. Limited research on strategies to eliminate pathogens in these environments are available. Rendered fat characteristics, such as water activity and fatty acids composition, may contribute to Salmonella survivability. The purpose of this research was to evaluate the effects of moisture levels (0%, 0.5%, 1%, and 3%), storage temperatures (48 and 76 ˚C), and fat characteristics on the growth and survival of Salmonella in beef tallow, white grease and poultry fat samples. Samples were inoculated with a high (~108 CFU/mL) and a low (~105 CFU/mL) Salmonella cocktail (S. Senftenberg, S. Newport, S. Thompson and S. Infantis). Samples were stored for up to 5 days at 48 and 76 ºC. Remaining population was evaluated daily with and without enrichment step. Death rates were calculated using Weibull model for each temperature and moisture level. Only temperature had an effect (P < 0.05) on Salmonella inactivation, while no effect between moisture and/or inoculum level were observed. When all products were challenged at 76 ˚C, counts were below detectable limits after 6 hours. At 48˚C a progressive decline in Salmonella population was observed within 3 days for both beef tallow and white grease when high inoculum was used for the challenge study. Salmonella was below detectable limit within 4 days for both fat types when a low inoculum was instead applied. This research identified the effect of moisture and temperature in rendered fat samples contaminated with Salmonella and underlined the need to use time–moisture–temperature data to minimize microbial growth during transportation and storage.
The protective benefit of heme oxygenase-1 gene modified human placenta-derived Mesenchymal stem cell in N-nitro-L-arginine methyl ester -induced preeclampsia-like rat model: Possible implications for placental angiogenesis.

We previously reported that cytoprotective heme oxygenase-1 (HO-1) gene-modified human placenta-derived Mesenchymal stem cell (PMSC) improved placental vascularization in vitro. In the current study, we explored the protective benefit of HO-1-PMSC transplantation in a preeclampsia (PE)-like rat model. A model of PE was successfully constructed by intraperitoneal injection of N-nitro-L-arginine methyl ester (L-NAME). Blood pressure and urinary protein levels were measured. Doppler ultrasound was examined to understand uteroplacental perfusion. ELISA was used to examine the serum levels of VEGF, PlGF, sFlt-1, and sEng. The placentas and fetuses were weighed to verify the improvement in pregnancy outcome. Immunohistochemical and H&E staining was used to detect microvascular density (MVD) in placental tissues and kidney pathology, respectively. The distribution of GFP-labeled PMSC in the placenta were observed under fluorescence microscopy. Blood pressure and proteinuria were reduced and kidney damage improved. PE rat models treated with PMSC and HO-1-PMSC exhibited an increase in the quality of fetuses and placentas, MVD, VEGF, and PlGF expression, but substantially decreased expression of sFlt-1 and sEng. Doppler ultrasound showed that the placental perfusion improved. Green fluorescent tracing experiments verified that the cells were successfully transplanted into the placenta and distributed in the blood vessels, indicating that the cells might participate in the process of angiogenesis. These results indicate that therapy with HO-1-PMSC could improve placental vascular dysplasia, increase placental perfusion, control PE symptoms, and promote pregnancy outcome by regulating the balance of angiogenic and anti-angiogenic factors or directly participating in the repair of placental vessels in a PE-like rat model.
Existence of Gender-Based Difference in Morphology of Convex Lateral Tibial Plateau in Korean Population Primary Knee Joint Osteoarthritis

Purpose Morphological differences in the knee joints of females and males have been reported in a previous study. These differences have realized the need of developing a gender-specific prosthesis. However, anatomical studies on gender-based differences in the proximal tibial plateau's sagittal curvature have rarely been conducted. Therefore, this study is aimed at evaluating the geometry of the sagittal curvature of the proximal tibial plateau in the Korean population. Methods Three-dimensional data for the sagittal curvature of the tibial plateau morphology from 1976 patients (i.e., 299 male and 1677 female) were assessed using magnetic resonance imaging. The sagittal profiles of the tibial plateaus were also evaluated. The independent t-test and paired t-test were used for statistical analysis. Results The proximal tibia had concave and convex surfaces in the medial and lateral plateaus, respectively, for both genders. In addition, the medial diameter of the tibial plateau was significantly greater than the lateral diameter for both genders. Gender-based difference was not found in the medial diameter of the tibial plateau but was observed in the lateral diameter. Conclusion These results may provide guidelines for a suitable knee implant design for the Korean patients. The incorporation of this shape information in the medial and lateral sides in the prosthetics for a total knee arthroplasty and a lateral unicompartmental knee arthroplasty can improve knee range motion.
In Situ Preservation of Parathyroid Gland With Vasculature for Papillary Thyroid Carcinoma Is Associated With Higher PTH Levels After Total Thyroidectomy

Purpose: To evaluate the impact of parathyroid gland vasculature preservation in-situ technique (PGVPIST) on postoperative parathyroid hormone (PTH) and calcium plasma levels in thyroid patients undergoing total thyroidectomy for papillary thyroid carcinoma (PTC). Study Design: Retrospective cohort study. Methods: Patients with PTC who underwent total thyroidectomy by either the conventional technique (group 1, January 2019 to January 2020) or PGVPIST (group 2, January 2020 to January 2021) were compared. Postoperative blood calcium levels and PTH levels were assessed in these groups. Results: Totally 149 patients with consecutive PTC underwent total thyroidectomy, including 60 patients in group 1 and 89 patients in group 2. Postoperative serum calcium levels in group 1 were insignificantly lower than in group 2 at day 1 (2.18 ± 0.02 vs 2.15 ± 0.01 mmol/L) and day 30 (2.27 ± 0.02 vs 2.38 ± 0.11) after surgery. But postoperative serum PTH levels in group 1 were significantly lower than that in group 2 at day 1 (23.68 ± 2.54 vs 31.46 ± 2.11 pg/mL) and day 30 (45.63 ± 3.21 vs 55.65 ± 2.89 pg/mL) after surgery. Conclusion: Parathyroid gland vasculature preservation in-situ technique for PTC is associated with higher PTH level after total thyroidectomy. The parathyroid gland vasculature mostly strongly adheres with adjacent thyroid parenchyma. Therefore, deferred processing of tiny thyroid parenchyma of parathyroid gland vessels is essential to prevent devascularization.
COVID-19 and Acute Coronary Syndromes: From Pathophysiology to Clinical Perspectives

Acute coronary syndromes (ACS) are frequently reported in patients with coronavirus disease 2019 (COVID-19) and may impact patient clinical course and mortality. Although the underlying pathogenesis remains unclear, several potential mechanisms have been hypothesized, including oxygen supply/demand imbalance, direct viral cellular damage, systemic inflammatory response with cytokine-mediated injury, microvascular thrombosis, and endothelial dysfunction. The severe hypoxic state, combined with other conditions frequently reported in COVID-19, namely sepsis, tachyarrhythmias, anemia, hypotension, and shock, can induce a myocardial damage due to the mismatch between oxygen supply and demand and results in type 2 myocardial infarction (MI). In addition, COVID-19 promotes atherosclerotic plaque instability and thrombus formation and may precipitate type 1 MI. Patients with severe disease often show decrease in platelets count, higher levels of d-dimer, ultralarge von Willebrand factor multimers, tissue factor, and prolongation of prothrombin time, which reflects a prothrombotic state. An endothelial dysfunction has been described as a consequence of the direct viral effects and of the hyperinflammatory environment. The expression of tissue factor, von Willebrand factor, thromboxane, and plasminogen activator inhibitor-1 promotes the prothrombotic status. In addition, endothelial cells generate superoxide anions, with enhanced local oxidative stress, and endothelin-1, which affects the vasodilator/vasoconstrictor balance and platelet aggregation. The optimal management of COVID-19 patients is a challenge both for logistic and clinical reasons. A deeper understanding of ACS pathophysiology may yield novel research insights and therapeutic perspectives in higher cardiovascular risk subjects with COVID-19.
The effects of cultural intelligence and Vietnamese proficiency on expatriate adjustment in Vietnam

cultural intelligence; expatriate; expatriate adjustment; hostcountry language proficiency; Vietnam The expatriate adjustment has been receiving more and more academic attention due to its increasing importance in globalization. There are many antecedents and outcomes of cross-cultural adjustment of expatriates. This research explores the relationships among Vietnamese proficiency, four dimensions of cultural intelligence, and expatriate adjustment. The study was conducted with data from 379 expatriates living/lived in Vietnam. The results reveal that Vietnamese proficiency and metacognitive cultural intelligence affect general adjustment indirectly through work and interaction adjustment. Meanwhile, cognitive cultural intelligence only a general adjustment but not the other two facets. The researcher also gives practical implications for corporations, international human resource management practitioners, and individual expatriates.
INFLUENCE OF COMPETENCE, INDEPENDENCE, SPIRITUAL QUOTIENT, EMOTIONAL QUOTIENT, AND AUDIT TENURE ON AUDIT QUALITY Ready Wicaksono*, Mispiyanti

Audit quality is the probability of an assessment given by the auditor about the discovery of a violation in the client's accounting system and reports the violation. The purpose of this study is to obtain empirical evidence of competence, independence, spiritual quotient, emotional quotient, and audit tenure variables on audit quality. This research is quantitative. The population in this study is the Public Accountant Office in Yogyakarta with the sample selection method that is convenience sampling. The data is taken from respondents' responses to positive and negative statements contained in the questionnaire. Data processing and hypothesis testing techniques using SPSS version 22. The results of this study prove that competence, independence, spiritual quotient, emotional quotient, and audit tenure have a significant positive effect on audit quality. This shows that an auditor must have good personal quality, adequate knowledge, and special expertise in his field, not influenced by other parties, and is obliged to be honest not only to the management and owners of the company but also users of financial statements, have a spiritual and emotional good, and a deeper and more complete understanding of the company's operational activities, along with the increase in the number of years of the audit engagement with the company so that it has an impact on the quality of audits produced.
Amino acid profiles and in vitro antioxidant properties of cereal-legume flour blends

Legumes and cereals stand a great chance as remedies to overcome incidences of protein malnutrition and oxidative stress. Cereal grains such as wheat (WT), millet (ML), maize (MZ) and acha (AC) were blended with cowpea (CP), peanut (PN) and soybean (SO) at varying levels to produce four different blends. Protein contents of the cereal-legume blends were determined and found to be higher as the inclusion level of legumes increased in the blends. Amino acid profiles and antioxidant properties of the flour blends were evaluated. Results showed that leucine was the most abundant (6.52–8.45 g/100 g) essential amino acid in the flour blends while total content of essential amino acids increased as the level of legume incorporation increased in the WT/SO (31.3–36.2 g/100 g) and MZ/CP (34.5–37.4 g/100 g). The antioxidant properties showed that MZ50:CP50 exhibited greater ferric reducing antioxidant power while WT70:SO30 and AC50:SO50 had stronger metal chelation activity and ML50:PN50 scavenged the most DPPH radicals when compared to the other flour blends. The results suggest that the composite flours have the potential to be used as ingredients for the formulation of food products with high levels of essential nutrients in addition to antioxidant benefits.