Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel Quinolone Nonsusceptible Streptococcus canis Strains with Point Mutations in Quinolone Resistance-determining Regions and Their Related Factors.

This study investigated quinolone nonsusceptible Streptococcus canis with point mutations in quinolone resistance-determining regions (QRDRs). After selecting targets from 185 isolates, we conducted antimicrobial susceptibility testing using levofloxacin, ciprofloxacin, norfloxacin, and moxifloxacin. We also determined amino acid sequences of QRDRs in gyrA/ gyrB/parC/parE genes and their point mutations. Finally, we performed S. canis-derived M-like protein (SCM) allele typing, multilocus sequence typing, and antimicrobial resistance genotyping. Correlations between nonsusceptible strains and their related factors were examined. We found 13 (7.0%) nonsusceptible isolates consisting of two classes, high-level minimum inhibitory concentrations (MICs) (n = 7, 3.8%), and low-level MICs (n = 6, 3.2%). The mutations Ser81Phe/Ser81Tyr/Glu85Lys in gyrA, Ser67Phe/Ser67Tyr/Asp71Tyr in parC, Asp438Asn in parE, and Gly408Asp in gyrB were observed in these nonsusceptible strains. The common mutations were Ser81 and Ser67/Asp71, whereas we found one strain each with Glu85, Asp438 and Gly408 mutations. There was a significant correlation between the nonsusceptible isolates and presence of SCM allele type 2, sequence type 46, tetracycline-resistance genes, and macrolide/lincosamide-resistance genes. These results could be used in the future by veterinarians while treating companion animals with clinical symptoms of streptococcal infections.
Análisis comparativo de la acción ofensiva en F-7 y F-8, en la categoría alevín

In the latest years, it has been considered, within the Spanish Football Federation, the possibility of creating a new league of 8 football�s game instead of 7 football�s game. This article tries to enlighten the subject, as an approximation to the attacking phase of 7 and 8 football�s game in the children category. Within the observational methodology, using contrastive statistics -with the software SPSS, version 15.0-, and with the detection of hidden sequential patterns -with the software Theme 5.0-, the features of the attacking process between both 7 and 8 football categories are compared. No meaningful differences have been found between both modalities, F-7 and F-8, in the observational data acquired, except in the ending phase
Postcyclic Undrained Strength for Cohesive Soils

The undrained strength of saturated cohesive soils after cyclic loading decreases and increases depending on whether the soil is initially normally consolidated or overconsolidated and whether drainage is included during or after cyclic loading. The methods for predicting these changes due to both strength degradation and its recovery in saturated cohesive soils after cyclic loading are presented in the current paper. The experimental parameters included in the proposed equations are easily determined as a function of plasticity index. The applicability of the methods is shown by investigating the results from both cyclic triaxial and direct simple shear tests on various kinds of normally consolidated cohesive soils including highly sensitive clays and peat. It is suggested from this consideration that they are valid for the prediction of the undrained strength for cohesive soils after cyclic loading with and without drainage.
Cerebellar white matter disruption in Alzheimer’s Disease patients: a Diffusion Tensor Imaging study

The cognitive role of the cerebellum has recently gained much attention, and its pivotal role in Alzheimer’s disease (AD) has now been widely recognized. Diffusion Tensor Imaging (DTI) has been used to evaluate the disruption of the microstructural milieu in AD, and though several white matter (WM) tracts such as corpus callosum, inferior and superior longitudinal fasciculus, cingulum, fornix and uncinate fasciculus have been evaluated in AD, data on cerebellar WM tracts are currently lacking. We performed a tractography-based DTI reconstruction of the middle cerebellar peduncle (MCP), and the left and right superior cerebellar peduncles separately (SCPL and SCPR) and addressed the differences in fractional anisotropy (FA), axial diffusivity (Dax), radial diffusivity (RD) and mean diffusivity (MD) in the three tracts between 50 patients with AD and 25 healthy subjects (HS). We found that AD patients showed a lower FA and a higher RD compared to HS in MCP, SCPL and SCPR. Moreover, a higher MD was found in SCPR and SCPL and a higher Dax in SCPL. This result is important as it challenges the traditional view that WM bundles in the cerebellum are unaffected in AD and might identify new targets for therapeutic interventions.
Low Serum Magnesium and the Development of Atrial Fibrillation in the Community The Framingham Heart Study Epidemiology and Prevention

incidence rate of AF was 9.4 per 1000 person-years (95% confidence interval, 6.7–11.9) in the lowest quartile of serum magnesium ( ≤ 1.77 mg/dL) compared with 6.3 per 1000 person-years (95% confidence interval, 4.1– 8.4) in the highest quartile ( ≥ 1.99 mg/dL). In multivariable-adjusted models, individuals in the lowest quartile of serum magnesium were ~ 50% more likely to develop AF (adjusted hazard ratio, 1.52; 95% confidence interval, 1.00–2.31; P =0.05) compared with those in the upper quartiles. Results were similar after the exclusion of individuals on diuretics. Conclusions —Low serum magnesium is moderately associated with the development of AF in individuals without cardiovascular disease. Because hypomagnesemia is common in the general population, a link with AF may have potential clinical implications. Further studies are warranted to confirm our findings and to elucidate the underlying mechanisms. ( Circulation. 2013;127:33-38.) surgery, or myocar- dial infarction and those on diuretic therapy at baseline. In further analyses, we tested for effect modification between moderate to heavy alcohol use and magnesium concentration and the risk of AF. We conducted a series of sensitivity analyses examining various approaches to categorizing magnesium concentrations, including examining the lowest quartile versus the upper 3 quartiles and the lowest decile versus the upper 9 deciles. We also performed a spline analysis to assess the linearity of the association between serum magnesium and AF risk. All analyses were
A Unified Framework for Event Summarization and Rare Event Detection from Multiple Views

A novel approach for event summarization and rare event detection is proposed. Unlike conventional methods that deal with event summarization and rare event detection independently, our method solves them in a single framework by transforming them into a graph editing problem. In our approach, a video is represented by a graph, each node of which indicates an event obtained by segmenting the video spatially and temporally. The edges between nodes describe the relationship between events. Based on the degree of relations, edges have different weights. After learning the graph structure, our method finds subgraphs that represent event summarization and rare events in the video by editing the graph, that is, merging its subgraphs or pruning its edges. The graph is edited to minimize a predefined energy model with the Markov Chain Monte Carlo (MCMC) method. The energy model consists of several parameters that represent the causality, frequency, and significance of events. We design a specific energy model that uses these parameters to satisfy each objective of event summarization and rare event detection. The proposed method is extended to obtain event summarization and rare event detection results across multiple videos captured from multiple views. For this purpose, the proposed method independently learns and edits each graph of individual videos for event summarization or rare event detection. Then, the method matches the extracted multiple graphs to each other, and constructs a single composite graph that represents event summarization or rare events from multiple views. Experimental results show that the proposed approach accurately summarizes multiple videos in a fully unsupervised manner. Moreover, the experiments demonstrate that the approach is advantageous in detecting rare transition of events.
Comprehensive Probabilistic Seismic Hazard Analysis of the Andaman–Nicobar Regions

An updated catalog of earthquakes has been prepared for the Andaman-Nicobar and adjoining regions. The catalog was homogenized to a unified magnitude scale, and declustering of the catalog was performed to remove aftershocks and foreshocks. Eleven regional source zones were identified in the study area to account for local variability in seismicity characteristics. The seismicity parameters were estimated for each of these source zones, and the seismic hazard evaluation of the Andaman-Nicobar region has been performed using different source models and attenuation relations. Probabilistic seismic hazard analysis has been performed with currently available data and their best possible scientific interpretation using an appropriate instrument such as the logic tree to explicitly account for epistemic uncertainty by considering alternative models (source models, maximum magnitude, and attenuation relationships). The hazard maps for different periods have been produced for horizontal ground motion on the bedrock level.
The adaptive benefit of evolved increases in hemoglobin-O 2 affinity is 2 contingent on tissue O 2 diffusing capacity in high-altitude deer mice 3

Oliver H. Wearing, Catherine M. Ivy, Natalia Gutiérrez-Pinto, Jonathan P. Velotta, Shane C. 4 Campbell-Staton, Chandrasekhar Natarajan, Zachary A. Cheviron, Jay F. Storz, and Graham 5 R. Scott 6 a Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada; b School of 7 Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA; c Department of Biological 8 Sciences, University of Denver, Denver CO 80210, USA; d Department of Ecology and 9 Evolutionary Biology, Institute for Society and Genetics, University of California, Los Angeles, CA 10 90095, USA; e Division of Biological Sciences, University of Montana, Missoula, MT 59812. 11 Corresponding author: Oliver H. Wearing 12 Corresponding author email: wearingo@mcmaster.ca 13 O.H. W. , 0000-0002-1866-0416; C.M. I. , 0000-0002-4713-4423; N.G. -P., 0000-0003-3684-5374; 14 S.C. C. -S. , 0000-0001-9778-7302; C.N. , 0000-0002-4841-0426; J.F. S. , 0000-0001-5448-7924; 15 G.R. S. , 0000-0002-4225-7475. 16
Dose-dependent vascular response following delivery of sirolimus via fast releasing, biodegradable polymer stent matrix: an experimental study in the porcine coronary model of restenosis

Background: Fast releasing, rapamycin-eluting stents, although safe, showed inferior results with regard to inhibition of restenosis. Aim: Therefore, we report vascular effects of a novel, biodegradable polymer stent matrix with elevated sirolimus dose and fast release kinetics (ed-frSES, Alex, Balton) in the porcine coronary in-stent restenosis model. Methods: A total of 19 stents were implanted with 120% overstretch in the coronary arteries of seven domestic pigs: seven ed-frSES with 1.3 μg/mm 2 of sirolimus, eight frSES with 1 μg/mm 2 of sirolimus, and eight bare metal stents (BMS). For the following 28 days, coronary angiography was performed, animals were sacrificed, and the stented segments harvested for histopathological evaluation. Results: In angiography at 28 days the late lumen loss was lowest in the elevated dose sirolimus eluting stent (SES) (ed-frSES: 0.20 ± 0.2 vs. frSES: 0.80 ± 0.5 vs. BMS: 0.96 ± 0.5 mm, p < 0.01). This was confirmed in the morphometric evaluation in histopathology as represented by a significant and dose-dependent decrease in the percentage area of stenosis (ed-frSES: 22.4 ± 12.7% vs. frSES: 35 ± 10.7% vs. BMS: 47.5 ± 12.5%, p < 0.01). There was no peri-strut inflammation in any of the groups. However, the endothelialisation score was numerically not meaningfully decreased in ed-frSES (ed-frSES: 2.93 vs. frSES: 3. vs. BMS: 3, p = 0.05). Signs of fibrin were also noted in ed-frSES (ed-frSES: 0.4 vs. frSES: 0 vs. BMS: 0, p = 0.05). Conclusions: Sirolimus dose-dependent vascular response was reported. The elevated dose, fast releasing SES shows satisfac-tory vascular healing, similar to regular dose, fast release SES, with improved efficacy in restenosis inhibition.
Antipsychotic treatment resistance in first-episode psychosis: prevalence, subtypes and predictors.

Background. We examined longitudinally the course and predictors of treatment resistance in a large cohort of ﬁ rst-episode psychosis (FEP) patients from initiation of antipsychotic treatment. We hypothesized that antipsychotic treatment resistance is: ( a ) present at illness onset; and ( b ) differentially associated with clinical and demographic factors. Method. The study sample comprised 323 FEP patients who were studied at ﬁ rst contact and at 10-year follow-up. We collated clinical information on severity of symptoms, antipsychotic medication and treatment adherence during the fol-low-up period to determine the presence, course and predictors of treatment resistance. Results. From the 23% of the patients, who were treatment resistant, 84% were treatment resistant from illness onset. Multivariable regression analysis revealed that diagnosis of schizophrenia, negative symptoms, younger age at onset, and longer duration of untreated psychosis predicted treatment resistance from illness onset. Conclusions. The striking majority of treatment-resistant patients do not respond to ﬁ rst-line antipsychotic treatment even at time of FEP. Clinicians must be alert to this subgroup of patients and consider clozapine treatment as early as possible during the ﬁ rst presentation of psychosis. remission following ﬁ rst two trials of antipsychotic treatment.
Feasibility study of a natural vinaigrette from olive (Olea europaea)
oil and lemon (Citrus limonium) juice-based lemon albedo extract

The present paper reports on the feasibility study of a natural vinaigrette from olive (Olea europaea) oil and lemon (Citrus limonium) juice-based lemon albedo extract. The albedo extract was prepared at different albedo to lemon juice ratios. The physical stability of different vinaigrette samples, in terms of creaming behaviour, was adopted as quality criterion. The most stable sample was then analysed for its rheological behaviour. According to preliminary results, the most physically stable vinaigrette could be obtained at albedo: lemon juice ratio of 1:15.1 (w/w). The corresponding dressing showed a stability of six days and a shear-thinning type rheological behaviour. So, the formulation of natural vinaigrette from olive oil, lemon juice and lemon albedo is feasible but such formulation is of short-term physical stability, which is a general well-known characteristic of natural and minimally processed food products. In our opinion, this study merits further research, in order to: i) better optimize the vinaigrette formulation, and ii) characterize the functional properties of the final product.
Faulting associated with historical and recent earthquakes in the Eastern Mediterranean region

SUMMARY This paper summarizes evidence for surface faulting in historical and recent earthquakes in the Eastern Mediterranean region and in the Middle East. Such information is particularly important for studies of active tectonics and for palaeoseismology. We have found 78 cases of faulting pre-1900 and 72 post-1900, some of which show that faults that have apparently been inactive this century had already ruptured before 1900. For some cases faulting could not have been predicted from 20th century activity, and in others it could have been expected, but has not been observed during the instrumental period. The data are suYcient to allow the derivation of relationships between magnitude and rupture length.
The nutrient status of the sediments of Hooghly estuary

Distribution of organic carbon, total nitrogen, available nitrogen, total phosphorus and available phosphorus have been studied in relation to the texture of the sediments of Hooghly estuary at three stations. The sediments at station 2 near Haldia Port showed high values for all the parameters compared to station 1 near Sagar Island. The organic carbon varied from 1.53 to 5.41 mg C/g, total nitrogen from 0.008 to 0.058X, available nitrogen from 9 to 32% of total nitrogen and available phosphorus from 0.5 to 9.1% of total phosphorus
High-quality annotation of promoter regions for 913 bacterial

Motivation: The number of bacterial genomes being sequenced is increasing very rapidly and hence, it is crucial to have procedures for rapid and reliable annotation of their functional elements such as promoter regions, which control the expression of each gene or each transcription unit of the genome. The present work addresses this requirement and presents a generic method applicable across organisms. Results: Relative stability of the DNA double helical sequences has been used to discriminate promoter regions from nonpromoter regions. Based on the difference in stability between neighboring regions, an algorithm has been implemented to predict promoter regions on a large scale over 913 microbial genome sequences. The average free energy values for the promoter regions as well as their downstream regions are found to differ, depending on their GC content. Threshold values to identify promoter regions have been derived using sequences flanking a subset of translation start sites from all microbial genomes and then used to predict promoters over the complete genome sequences. An average recall value of 72% (which indicates the percentage of protein and RNA coding genes with predicted promoter regions assigned to them) and precision of 56% is achieved over the 913 microbial genome dataset.
Characterisation of endogenous Claudin-1 expression, motility and susceptibility to hepatitis C virus in CRISPR knock-in cells

Background Information. Claudin1 (CLDN1) is a four-span transmembrane protein localized at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homeostasis under physiological conditions. Moreover, CLDN1 expression is upregulated in several cancers, and the level of CLDN1 expression has been proposed as a prognostic marker of patient survival.
Explorer Analysis of conformational stability of abnormal prion protein aggregates across the spectrum of Creutzfeldt-Jakob disease

The wide phenotypic variability of prion diseases is thought to depend on the interaction of a 25 host genotype with prion strains that have self-perpetuating biological properties enciphered in 26 distinct conformations of the misfolded prion protein, PrP Sc . The latter concept is largely based 27 on indirect approaches studying the effect of proteases or denaturing agents on the 28 physicochemical properties of PrP Sc aggregates. Furthermore, most data come from studies on 29 rodent-adapted prion strains, making current understanding of the molecular basis of strains and 30 phenotypic variability in naturally occurring diseases, especially in humans, more limited. To fill 31 this gap, we studied the effect of guanidine hydrochloride (GdnHCl) and heating on PrP Sc 32 aggregates extracted from 60 sporadic CJD and 6 variant CJD brains. While denaturation curves 33 obtained after exposure of PrP Sc to increasing GndHCl concentrations showed a similar profile 34 among the 7 CJD types analysed, PrP Sc exposure to increasing temperature revealed significantly 35 different and type-specific responses. In particular, MM1 and VV2, the most prevalent and faster 36 replicating CJD types, showed stable and highly resistant PrP Sc aggregates, whereas VV1, a rare 37 and slow propagating type, revealed unstable aggregates that easily dissolved at low temperature. 38 Taken together our results indicate that the molecular interactions mediating the aggregation 39 state of PrP Sc , possibly enciphering strain diversity, are differently targeted by GdnHCl, 40 temperature and proteases. Furthermore, the detected positive correlation between thermo- 41 stability of PrP Sc aggregates and disease transmission efficiency makes inconsistent the proposed 42 hypothesis that a decrease in conformational stability of prions results in an increase in their 43 replication efficiency. direct the strain replication efficiency and the thermo-stability of prion protein aggregates. In carried out the first systematic analysis of Sc stability in a large series of samples across the whole spectrum of human and vCJD prions. Our results failed to reveal significant strain-specific differences in the PrP aggregates. Thus, the limitations related to the crude, the suggest that
Natural electromagnetic pulses in the ELF range

We analyze the propagation of electromagnetic waves in the ELF. We apply the modal solution for the spherical Earth‐ionosphere cavity and obtain the time domain fields using the FFT algorithm. The Schumann resonance amplitude over the frequency‐distance plane consists of summits and depressions exhibiting separate extended zones. When the source‐observer distance is small, the field maximum is found at some hundred hertz. This component rapidly decays with the distance. The time domain ELF pulse travels with a constant velocity and “bounces” from the source antipode. Its width increases along the path due to absorption of the high frequency component. The distance dependence of the pulse amplitude depends on both the geometrical focusing and the high frequency absorption.
Pharmacokinetic profiles of boosted darunavir, dolutegravir and lamivudine in aging people living with HIV

Results: Nineteen PLWH with a median age of 64 years participated in the full pharmacokinetic investigations. Single point pharmacokinetic data were collected for 804 PLWH with a median age of 52 years. Boosted darunavir clearance was 40% lower in aging ( 65 years) compared with younger (<65 years) PLWH, consistent with other drugs predominantly metabolized by CYP3A. Dolutegravir exposure was similar between age groups whereas lamivudine exposure increased by 11% in aging PLWH. Median boosted darunavir, dolutegravir and lamivudine t1/2 were 148%, 45% and 32% higher in aging compared with younger PLWH.
The discovery of the Barmer Basin, Rajasthan, India, and its petroleum geology

The discovery of oil and gas in the Barmer Basin in northwest India was one of the more significant global discoveries in the decade 2001–2010. The basin’s presence was suspected from gravity and magnetic data in the late 1980s but not confirmed until 1999 from seismic and drilling. The basin is a lacustrine failed rift. Biostratigraphic data, however, indicate it was intermittently connected to marine waters via either the Cambay Basin, the Kutch Basin, or across the Devikot high, temporarily forming a large, shallow estuary. At least six major tectono-stratigraphic events have caused relative lake level falls and translation of clastic reservoirs basinward. Upward of 6 km (∼20,000 ft) of Cenozoic and Mesozoic sedimentary rocks have been preserved. Prolific source rocks occur from the Mesozoic through Eocene strata. Tectonically, the basin is divided into a northern and a southern province. The north province continues to undergo inversion and erosion, and has not been buried as deeply as the south. Kinetics of the major source facies in the north are substantially different from those in the south, as well as the present-day and paleo-heat flow. These differences have made the northern part of the basin predominantly an oil province and the southern part a mixed oil and gas province. The prolific Paleocene Fatehgarh Formation contains the bulk of the 7.3 billion barrels of stock tank oil in place (STOIP) identified to date, but other reservoirs from the Mesozoic to the late Cenozoic are common and may yield significant future resource additions.