Miracle-dz/UniFAR-FTUs huggingface dataset:
Severity of Clinical Mastitis and Bacterial Shedding

The aim of this cross-sectional study was to investigate associated factors of the severity of clinical mastitis (CM). Milk samples of 249 cases of CM were microbiologically examined, of which 27.2% were mild, 38.5% moderate, and 34.3% severe mastitis. The samples were incubated aerobically and anaerobically to investigate the role of aerobic and anaerobic microorganisms. In addition, the pathogen shedding was quantitatively examined, and animal individual data, outside temperature and relative humidity, were collected to determine associated factors for the severity of CM. The pathogen isolated the most was Escherichia coli (35.2%), followed by Streptococcus spp. (16.4%). Non-aureus staphylococci (NaS) (15.4%) and other pathogens (e.g., Staphylococcus aureus, coryneforms) (15.4%) were the pathogens that were isolated the most for mild mastitis. Moderate mastitis was mostly caused by E. coli (38%). E. coli was also the most common pathogen in severe mastitis (50.6%), followed by Streptococcus spp. (16.4%), and Klebsiella spp. (10.3%). Obligate anaerobes (Clostridium spp.) were isolated in one case (0.4%) of moderate mastitis. The mortality rate (deceased or culled due to the mastitis in the following two weeks) was 34.5% for severe mastitis, 21.7% for moderate mastitis, and 4.4% for mild mastitis. The overall mortality rate of CM was 21.1%. The pathogen shedding (back logarithmized) was highest for severe mastitis (55,000 cfu/mL) and E. coli (91,200 cfu/mL). High pathogen shedding, low previous somatic cell count (SCC) before mastitis, high outside temperature, and high humidity were associated with severe courses of mastitis.
A Theoretical Investigation of Novel Sila- and Germa-Spirocyclic Imines and Their Relevance for Electron-Transporting Materials and Drug Discovery

A new class of spirocyclic imines (SCIs) has been theoretically investigated by applying a variety of quantum chemical methods and basis sets. The uniqueness of these compounds is depicted by various peculiarities, e.g., the incidence of planar six-membered rings each with two imine groups (two π bonds) and the incorporation of the isosteres carbon, silicon, or germanium spiro centers. Additional peculiarities of these novel SCIs are mirrored by their three-dimensionality, the simultaneous occurrence of nucleophilic and electrophilic centers, and the cross-hyperconjugative (spiro-conjugation) interactions, which provoke charge mobility along the spirocyclic scaffold. Substitution of SCIs with strong electron-withdrawing substituents, like the cyano group or fluorine, enhances their docking capability and impacts their reactivity and charge mobility. To gain thorough knowledge about the molecular properties of these SCIs, their structures have been optimized and various quantum chemical concepts and models were applied, e.g., full NBO analysis and the frontier molecular orbitals (FMOs) theory (HOMO-LUMO energy gap) and the chemical reactivity descriptors derived from them. For the assessment of the charge density distribution along the SCI framework, additional complementary quantum chemical methods were used, e.g., molecular electrostatic potential (MESP) and Bader’s QTAIM. Additionally, using the aromaticity index NICS (nuclear independent chemical shift) and other criteria, it could be shown that the investigated cross-hyperconjugated sila and germa SCIs are spiro-aromatics of the Heilbronner Craig-type Möbius aromaticity.
Salmonella Typhimurium. Epithelial NAIPs protect against

paralogs (Naip-6  /  ), we show that NAIPs are key regulators of colorectal tumorigenesis. Naip-6  /  mice developed increased colorectal tumors, in an epithelial-intrinsic manner, in a model of colitis-associated cancer. Increased tumorigenesis, however, was not driven by an exacerbated inflammatory response. Instead, Naip-6  /  mice were protected from severe colitis and displayed increased antiapoptotic and proliferation-related gene expression. Naip-6  /  mice also displayed increased tumorigenesis in an inflammation-independent model of colorectal cancer. Moreover, Naip-6  /  mice, but not Nlrc4-null mice, displayed hyper-activation of STAT3 and failed to activate p53 8 h after carcinogen exposure. This suggests that NAIPs protect against tumor initiation in the colon by promot-ing the removal of carcinogen-elicited epithelium, likely in a NLRC4 inflammasome-independent manner. Collectively, we demonstrate a
Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation

Simple Summary The basilar artery is a vital cerebral blood vessel common in most vertebrates and constantly supplies blood to the hindbrain where many vital functions are coordinated. Avian basilar arterial responsiveness to vasoactive substances has been characterized only in chickens. In this artery, the endothelium plays an important role in relaxation, and endothelial dependence may explain the lethality of the highly pathogenic avian influenza virus, which reportedly induces apoptosis in the cerebrovascular endothelium. Our present results in ducks suggest a contrast to the previously reported results in chickens with regard to basilar arterial relaxation: The involvement of endothelial nitric oxide as a relaxing factor appears to be reduced in duck basilar arteries. Our research may help scientists to better understand the resistance to the highly pathogenic avian influenza virus that may be conferred by the cerebrovascular endothelium in ducks. Abstract The basilar arterial endothelium mediates blood vessel relaxation partly through the release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to a high mortality rate in chickens infected with the highly pathogenic avian influenza virus, but interestingly, ducks exhibit a greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated with 5-HT1 and H1 receptors, while relaxation is induced with β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated with M3 and OK receptors but not β3-adrenergic receptors in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain the differences in lethality between ducks and chickens when vascular endothelial cells are infected with the virus.
The efficacy and safety of duloxetine for the treatment of patients after TKA or THA: A systematic review and meta-analysis

Background: Duloxetine, a serotonin–norepinephrine dual reuptake inhibitor, may improve analgesia after total joint arthroplasty (TJA). However, there is still no consensus on its effectiveness and safety. We conducted the meta-analysis to investigate the analgesic effect and safety of duloxetine for the treatment of patients received total knee or hip arthroplasty. Methods: Pubmed, Cochrane Central Registry for Clinical Trials, Embase, OVID, Web of Science, and Google Scholar were searched using a predetermined search strategy from inception to September 21, 2022. Only randomized controlled trials of duloxetine in treatment of patients after total knee or hip arthroplasty were included. Data collection and extraction, quality assessment, and data analyses were performed according to the Cochrane standards. Results: A total of 8 randomized controlled trials with 739 patients were included in the literature review of postoperative pain and adverse effects. The result of meta-analysis showed statistically significant lower opioid requirement with duloxetine (P < .05) for the different postoperative period. Duloxetine group had significant reductions in visual analog score for the 24-hour (walking: WMD = −0.98; 95% confidence interval [CI] = −1.69 to −0.26, P = .007; resting: WMD = −1.06; 95%CI = −1.85 to −0.27, P = .008) and 1-week (walking: WMD = −0.96; 95%CI = −1.42 to −0.50, P < .001; resting: WMD = −0.69; 95%CI = −1.22 to −0.16, P = .01); knee injury and osteoarthritis outcome score over 3-month (WMD = 2.94; 95%CI = −0.30 to 6.18, P = .008) and complication (odds ratio = 4.74; 95%CI = 0.23 to 96.56, P = .01) postoperative period compared with the control group. However, no difference on numeric rating scale (P > .05) for the different postoperative period; visual analog score (P > .05) for the 6-week or 3-month and knee injury and osteoarthritis outcome score (P > .05) for the 6-week postoperative period. Furthermore, it did not increase the incidence of adverse effects (odds ratio = 0.87; 95%CI = 0.72 to 1.05, P = .15). Conclusion: Duloxetine could decrease the opioids consumption and relieve early postoperative pain without increasing the risk of adverse medication effects in patients undergoing total knee or hip arthroplasty. Considering the ongoing opioid epidemic, duloxetine could act as a good supplement in multimodal pain management protocol for patients undergoing total joint arthroplasty.
Robotically Assisted Total Knee Arthroplasty - Literature Review

Despite the evolution in implant design, surgical technique, rehabilitation and better medical training, a great number of patients are not satisfied with the final result of Total Knee Arthroplasty (TKA). The conventional TKA technique could result in a high number of outliers in limb alignment and are related to a high rate of residual symptoms. Robotically assisted (RA) surgery was introduced to improve these results. Recent studies showed this technique is associated to lower costs, better accuracy in implant placement and limb alignment, improvement in function and patient satisfaction. RA technique is also related to less blood loss, less bone resection and smaller aggression to soft tissues. Besides that, this brings better ergonomics to the surgeon with less caloric waste. In this way, by the benefits showed, the adhesion to the robotic surgery must be encouraged.
The Nighttime Fragrance of Guettarda scabra (Rubiaceae): Flower Scent and Its Implications for Moth Pollination

Floral scent is crucial for attracting pollinators, especially in plants that bloom at night. However, chemical profiles of flowers from nocturnal plants with varied floral morphs are poorly documented, limiting our understanding of their pollination ecology. We investigated the floral scent in Guettarda scabra (L.) Vent. (Rubiaceae), a night-blooming species with short- and long-styled floral morphs, found in the threatened pine rocklands in south Florida, US. By using dynamic headspace sampling and GC–MS analysis, we characterized the chemical profiles of the floral scent in both morphs. Neutral red staining was also employed to determine the specific floral regions responsible for scent emission in G. scabra. The results revealed that G. scabra’s fragrance consists entirely of benzenoid and terpenoid compounds, with benzeneacetaldehyde and (E)-β-ocimene as dominant components. There were no differences in the chemical profiles between the long- and short-styled flowers. Staining assays indicated that the corolla lobes, anthers, and stigma were the primary sources of the scent. These findings indicate that G. scabra’s floral scent is consistent with that of night-blooming plants pollinated by nocturnal hawkmoths, providing important insights into its chemical ecology and pollinator attraction. This study demonstrates how floral scent chemistry can validate predictions based on flower morphology in hawkmoth-pollinated plants.
Management and mindset for air leak and intraoperative bleeding in thoracic surgery

(5). Therefore, effective management of air leaks is crucial. Malapert et al. demonstrated that the use of surgical sealants and buttressing materials reduces the risk of prolonged air leakage after lung resection and decreases the incidence of postoperative arrhythmias (6). They highlighted the usefulness of adhesives, patches, and buttressing. Additionally, Deguchi et al. emphasized the role of reinforced staplers in preventing PAL, especially in patients with emphysematous lungs (7).
Learning environment and academic success of students in public secondary schools in Imo State

This study examines the relationship between learning environment and academic performance of students in Imo State's public secondary schools. The ex-post-facto study design was adopted. A simple sampling strategy was used to select 155 respondents (50% of the population), from a group of 309 principals. To collect information from respondents, questionnaire survey called "Atmosphere of Learning was used". Also, a checklist was used to compile information on kids' academic success. The questionnaire was validated using face and content validity. Additionally, a Cronbach’s alpha reliability test was performed, and the findings revealed a high reliability index with a score of 0.71. The Pearson product moment correlation statistic was used to establish the relationship at 0.05 level of significant. Results showed that in Imo State, classroom setting, physical environment, learning atmosphere, and academic success of students were substantially associated. Principals in Imo State, Nigeria, are advised to make sure that classrooms are typically conducive to teaching and learning. The classroom should be set up so that it can hold the required number of pupils in order to facilitate effective teaching and learning.
Prognostic Impact of Percutaneous Coronary Intervention in Older Patients Hospitalized with Acute Myocardial Infarction: Real-World Findings from the Lombardy Health Database

Background. Older patients are less likely to receive percutaneous coronary intervention (PCI) for acute myocardial infarction (AMI) compared to younger patients. We investigated the prognostic impact of PCI in a large population of patients hospitalized with AMI in the period 2003–2018 by using the administrative Lombardy Health Database (Italy). Methods. We considered all patients aged ≥75 years hospitalized with AMI (either STEMI or NSTEMI) from 2003 to 2018 in Lombardy. Patients were grouped according to whether they were treated or not with PCI during the index hospitalization. The primary outcome was in-hospital mortality. The secondary endpoints were 1-year mortality and 1-year re-hospitalization for acute heart failure (AHF) or AMI. Results. 116,063 patients aged ≥75 years (mean age 83 ± 6; 48% males; 46% STEMI) were hospitalized with a primary diagnosis of AMI. Thirty-seven percent of them (n = 42,912) underwent PCI. The in-hospital mortality rate was significantly lower in PCI-treated patients (6% vs. 15%; p < 0.0001). One-year mortality and 1-year re-hospitalization for AHF/AMI were less frequent in PCI-treated patients (16% vs. 41% and 15% vs. 21%, respectively; p < 0.0001). The adjusted risks of the study endpoints were lower in PCI-treated patients: OR 0.37 (95% CI 0.36–0.39) for in-hospital mortality; HR 0.37 (95% CI 0.36–0.38) for 1-year mortality; HR 0.74 (95% CI 0.71–0.77) for 1-year re-hospitalization for AHF/AMI. Similar results were found in STEMI and NSTEMI patients considered separately. Conclusions. Our real-world data showed that in patients with AMI ≥ 75 years of age, PCI use is associated with lower in-hospital and 1-year mortality.
Overexpression of Insulin-Like Growth Factor 1 Enhanced the Osteogenic Capability of Aging Bone Marrow Mesenchymal Stem Cells

Many studies have indicated that loss of the osteoblastogenic potential in bone marrow mesenchymal stem cells (bmMSCs) is the major component in the etiology of the aging-related bone deficit. But how the bmMSCs lose osteogenic capability in aging is unclear. Using 2-dimentional cultures, we examined the dose response of human bmMSCs, isolated from adult and aged donors, to exogenous insulin-like growth factor 1 (IGF-1), a growth factor regulating bone formation. The data showed that the mitogenic activity and the osteoblastogenic potential of bmMSCs in response to IGF-1 were impaired with aging, whereas higher doses of IGF-1 increased the proliferation rate and osteogenic potential of aging bmMSCs. Subsequently, we seeded IGF-1-overexpressing aging bmMSCs into calcium-alginate scaffolds and incubated in a bioreactor with constant perfusion for varying time periods to examine the effect of IGF-1 overexpression to the bone-forming capability of aging bmMSCs. We found that IGF-1 overexpression in aging bmMSCs facilitated the formation of cell clusters in scaffolds, increased the cell survival inside the cell clusters, induced the expression of osteoblast markers, and enhanced the biomineralization of cell clusters. These results indicated that IGF-1 overexpression enhanced cells' osteogenic capability. Thus, our data suggest that the aging-related loss of osteogenic potential in bmMSCs can be attributed in part to the impairment in bmMSCs' IGF-1 signaling, and support possible application of IGF-1-overexpressing autologous bmMSCs in repairing bone defect of the elderly and in producing bone graft materials for repairing large scale bone injury in the elderly.
Antibiotic susceptibility profiles of Gram-negative bacterial uropathogens in a tertiary hospital, southwest Nigeria

Background: Increasing rates of antibiotic resistance have made it necessary to regularly monitor antibiotic susceptibility patterns of gram negative bacterial uropathogens in order to optimize antibiotic therapy for urinary tract infections. The aim of this study was to analyze the antibiotic susceptibility patterns of Gram-negative bacterial uropathogens in Babcock University Teaching Hospital, Ilishan-Remo, southwest Nigeria. Methodology: This study was a retrospective review of the Medical Microbiology Laboratory records of the hospital to analyze the in vitro antibiotic susceptibility patterns of Gram-negative urinary bacterial isolates between May 2016 and April 2022. The bacteria were isolated and identified from routine urine samples using standard bacteriological methods. In vitro antibiotic susceptibility test (AST) to amoxicillin-clavulanate, piperacillin-tazobactam, ceftriaxone, ceftazidime, nitrofurantoin, ciprofloxacin and meropenem was routinely performed by the modified Kirby-Bauer disk diffusion test and susceptibility break points determined using the Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total number of 3,549 urine samples were processed during the period of review, and 808 (22.8%) samples yielded positive bacterial cultures. Of the 808 isolates, 604 (74.8%) were Gram-negative bacteria. The most frequently isolated Gram-negative bacteria were Escherichia coli (41.9 %) and Klebsiella spp (27.5%) while Pseudomonas spp and Proteus spp accounted for 4.3% and 1.0% of all isolates respectively. Meropenem had the highest in vitro antibacterial activity (74.3% to 90.3% of isolates were sensitive) for all isolates. Overall, E. coli, Klebsiella spp. , and Proteus spp. showed high resistance rates to amoxicillin-clavulanate (65.3% to 97.1%). Conclusion: Effective antimicrobial stewardship programs must be in place in order to ensure the appropriate use of antibiotics for treating urinary tract infections.
Correlation between body mass index and quadruple hamstring autograft size

Objectives: Anterior cruciate ligament (ACL) tear is a common sports injury affecting the knee joint in the Middle East and worldwide. Providing an adequate graft is required to prevent revision surgery. This study aims to determine if the body mass index (BMI) can be used as a predictor of the hamstring autograft size. Methods: Data of 48 consecutive patients, who underwent ACL reconstruction using quadruple hamstring autograft technique in the same institute by the same surgeon, were retrospectively reviewed. Skeletally immature patients and those who underwent revision ACL surgeries were excluded. Patients' anthropometric measurements were collected. Quantitative data from the patients' charts were included in the descriptive statistics. Results: The mean age of our cohort was 30.3 ± 6.3 years, with a mean height of 1.7 ± 7.8 m and a mean weight of 81.3 ± 14.6 kg. Mean BMI was 27.8 ± 5, and mean graft size was 7.8 ± 0.5 mm. Results showed that the correlation between the BMI and the autograft size was statistically insignificant. Other variables have no association with the autograft size in the linear regression model. Conclusion: Our study showed no correlation between anthropometric measurements and the autograft size; therefore, these measurements were not able to determine the diameter of the autograft. Further studies looking into thigh diameter and ethnicity and radiological studies with a larger sample size are recommended.
Factors associated with cesarean sections in Brazilian hospitals.

OBJECTIVE To assess the prevalence of cesarean sections in Brazilian hospitals. METHODS A cross-sectional study was carried out with data from the World Health Organization's Global Data System for Maternal and Perinatal Health, for the Brazilian states of São Paulo, Pernambuco and the Federal District. Data relating to 15,354 women who gave birth between September/2004 and March/2005 were analyzed, according to sociodemographic, reproductive, and hospital-related characteristics. Bivariate analyses - with calculations of the prevalence ratios and respective confidence intervals - and multivariate Poisson regression analyses were performed. RESULTS The prevalence ratio of cesarean sections was significantly higher among older women, who were married/living with a partner and with higher body mass index. The following conditions during pregnancy or birth were associated with higher cesarean section prevalence ratio: parturient being diagnosed as HIV-positive, heavier weight and greater head circumference of the newborn, and more prenatal consultations. In regression analysis, the following variables showed direct association with the outcome: parturient being older and with higher schooling level, presence of hypertension/eclampsia, chronic condition or some other medical condition, newborn's greater head circumference, being primiparous, having had a cesarean in the last pregnancy and having received an epidural block or rachidian analgesic during labor. Although the proportion of cesareans was higher in hospitals with a high complexity index, the difference was not statistically significant, as well as for other characteristics of hospitals. CONCLUSIONS The conditions of the pregnancy, newborn and the sociodemographic and reproductive characteristics of the parturient were independently associated with cesarean delivery. The hospital complexity index was not associated with cesarean delivery, probably due to the homogeneity of the hospital sample.