Miracle-dz/UniFAR-FTUs huggingface dataset:
SPECTRUM OF CERVICAL LESIONS IN CIMS, BILASPUR: A 5 YEAR RETROSPECTIVE STUDY OF 215 CASES IN A TERTIARY HOSPITAL OF CENTRAL INDIA

: BACKGROUND AND OBJECTIVE: Cancer of the cervix is a leading cause of morbidity and mortality among women World Wide. Therefore to curb the disease there is need of awareness of this disease. This study is aimed to categorize the premalignant and malignant lesions at the earliest, to reduce the mortality and morbidity. It also aimed to know the incidence of malignancies at our set up and to calculate distribution of patient in relation to parity and symptoms. DESIGN AND SETTING: Study included 215 patients with complain of white discharge per vagina, per vaginal bleeding and backache attending the gynecology OPD over a period of 05 years. PATIENT AND METHODS: Patients were subjected to cervical biopsy and biopsies were sent to pathology department. Detailed clinical history, age, age at marriage, parity and socio-economic status was obtained. RESULT: Result were classified histopathologically as inflammatory lesions 70 cases, polypoidal lesion were 30, LSIL accounted for 32 cases and HSIL were 20 cases and 30 were malignant lesions. Under malignant category moderately differentiated squamous cell carcinoma was the commonest. Maximum numbers of patients were subjected to cervical biopsies in 3 rd decade of life. Malignancies were common in 3 rd & 4 th decade of life. Most of the cases were of parity 4. CONCLUSION: Tissue biopsy is a valuable diagnostic procedure on which surgeons and radiologists still rely. and due the incidence of cervical MATERIAL: Total hysterectomy patient were excluded from study. In this study detailed menstrual, obstetrics and contraceptive history was taken. Age of the patient, duaration of marriage, parity and socio econamic status was also noted. Chief complaints of the patient like while discharge per vagina, bachche, abdominal pain and per vaginal bleeding were also considered. Cervial biopsies were carried with help of punch biopsy forceps in operation theatre. Some biopsies were carried out under the guidence of colposcopy. The biopsies were sent to pathology department for histopathological examination. The sections were stained with Haematoxylin and Eosin and then examined. Results were categorized under inflamatory LSIS, (low grade squamons Intraepithelial lesion) HSIS (High grade squamons Intraepithelial lesion) polyps and malignancies. Age incidence, parity and distribution of cases in relation to symptoms was calculated. RESULTS: Out of 215 cervical biopsies examined 20 biopsies were inadequate for diagnosis and remaining 195 cases were histopathologically classified as inflammatory lesions i.e. chronic Non-Specific cervicitis 70 cases (35.89%), low grade squamous intraepithelial lesions (LSIL) 32 cases (16.14%), High grade
Community of Aphyllophorales and Root Rot in Stumps of Picea abies on Clear-felled Forest Sites in Lithuania

The community of Aphyllophorales fungi in stumps of Picea abies (L.) Karst. and the occurrence of root rot caused by Heterobasidion annosum were investigated at 38 clear-felled sites in Lithuania. Fruit-bodies were recorded on 36.0% of the 3924 examined stumps. The most common were Bjerkandera adusta, Phlebiopsis gigantea, Fomitopsis pinicola, Gloeophyllum sepiarium, Stereum sanguinolentum, Trametes zonata and H. annosum. All species occurred in stumps both with and without established root rot, but B. adusta, F. pinicola, G. sepiarium, T. zonata and Trichaptum abietinum occurred less often in stumps containing root rot, while H. annosum was encountered more often. Greater numbers of fruit-bodies were found on stumps cut in summer than on those cut in winter. The fruiting became most abundant 3-4 yrs after the trees were felled. The abundance of nearly all Aphyllophorales correlated positively with the stump diameter, and on larger stumps, fruit-bodies of several different fungi were more common. The number of stumps containing H. annosum root rot in different sites varied from 9.8% to 68.8%, and was 27.6% on average. The incidence of root rot correlated neither with the age nor with the density of the felled stand, but it correlated negatively with the proportion of deciduous trees within a stand.
Áhrif valinna stökkbreytinga á alkalískan fosfatasa úr sjávarlífverunni Vibrio splenditus í leit að fjölvirkni (e. promiscuity)

Organisms in a permanently cold environment need to synthesize cold – active enzymes to sustain proper metabolism. Alkaline phosphatase (AP) from the marine Vibrio bacterium (VAP) is an example of such a cold – active enzyme. APs are nonspecific catalysts that catalyse hydrolysis or transesterification of phosphoryl esters. There is also some evidence that APs are promiscuous in the sense that they can catalyse another type of chemical reaction than they originally evolved to do which make them promiscuous. In this study we looked at the effects of residue replacements by site – directed mutagenesis that were done in search of promiscuity in VAP. According to in silico analysis the replacement of four residues might suffice to change VAP into a sulfatase, T112A/R113E/H116D/W274K. Here the VAP variants T112A/R113G and H116D were produced. Rather than replacing arginine (R) with glutamic acid (E) it was decided to replace it with glycine (G) to get comparison with a small, neutral amino acid. After the mutagenesis T112A/R113G the VAP proved to be less stable at a high temperature (lower T50%) than the wild type and it had a lower affinity for the substrate (higher Km). T112A/R113G had a similar turnover number (kcat) as the wild type but less catalytic efficiency (kcat/Km). The thermal stability of the overall structure (Tm) of T112A/R113G was also less and that is presumably due to more flexibility of the enzyme. H116D had less affinity for the substrate compared to the wild type just as T112A/R113G. The catalytic efficiency of H11D was 30x less compared to the wild type. However, H116D proved to be more stable at a high temperature than both the wild type and T112A/R113G. We carried out two of the four mutations that are needed to awake sulfatase activity in VAP according to in silico analysis. These two were not enough to promote promiscuity in VAP.
Produtividade e estado nutricional do quiabeiro em função da densidade populacional e do biofertilizante suíno

The objective of this work was to evaluate yield and nutritional status of organically cultivated Santa Cruz okra as a function of planting densities and doses of swine biofertilizer. The experiment was carried out at the EPAMIG Experimental Farm, in Oratorios-MG, Brazil, from 16/10/2007 to 22/02/2008. The experiment was arranged in randomized blocks, with four replications, in a 2 x 5 factorial, with two plant populations and five doses of biofertilizer obtained by the fermentation of liquid swine waste. The two populations were 23809 and 35714 plants.ha-1, obtained in the 1.4 x 0.30 m spacing with a plant per hole and 1.4 x 0.40 m with two plants per hole. Biofertilizer doses were: 0; 6; 12; 24 and 48 m3.ha-1, with 80% applied in furrow 15 days before transplantation and 20% top-dressing at 30 days after seedling transplantation. The characteristics evaluated included nutrient leaf content, SPAD index, plant height, number of stems and production of commercial and non-commercial fruits. The use o the swine biofertilizer gave plants with a good nutritional status. The SPAD index correlated positively with N leaf content, with the highest concentrations at the highest doses of the swine biofertilizer, mainly in the smallest plant population. The number and commercial fruit production per plant were larger when a smaller plant population was used. The highest yield achieved in the population of 35714 plants ha-1 was 31.23 ton ha-1, and in the population of 23809 plant.ha-1 was 21.9 ton ha-1, both at the highest dose of biofertilizer. The highest commercial yield of okra fruits was obtained with the largest plant population.
Improved palatability of channel catfish feeds containing Romet-30.

Abstract Studies were conducted in the laboratory and in ponds to further evaluate the palatability of Romet-30® (5:1, sulfadimethoxine : Ormetoprim) to channel catfish Ictalurus punctatus and to devise methods to improve the palatability of feeds containing this drug. Healthy channel catfish were used in all experiments. Studies conducted in the laboratory indicated that the palatability of diets containing ormetoprim at a rate equivalent to 0.82% Romet-30 to age-0 (10–12g) fish was poor. Additionally, palatability decreased as the level of Romet-30 increased in the feed. In the remaining studies, 1.65% Romet-30 (one-half of the amount originally used in commercial catfish feeds) was used in all experimental feeds. Adjusting the pH of the feed to 7.0 did not improve palatability, nor did the addition of sucrose to the feed. Increasing the level of menhaden fish meal in medicated feeds to 16% appeared to improve palatability. Data from a preference trial conducted in ponds with demand feeders indicated th.
Exogenous Application of Glycinebetaine Modulates Activities of Antioxidants in Maize Plants Subjected to Salt Stress

The influence of exogenously applied glycinebetaine (0, 50 and 100 mM) as a foliar spray at different growth stages, i.e. vegetative, reproductive or both at the vegetative and reproductive stages on gas exchange characteristics, glycinebetaine (GB) and the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) was examined in plants of two maize cultivars, Golden and C-20 grown under saline conditions. Salt stress caused a marked decrease in photosynthetic capacity, chlorophyll contents and SOD activity in both maize cultivars. However, activities of CAT and POD remained almost unchanged in both maize cultivars under salt stress. Accumulation of GB increased with an increase in exogenous level of GB, i.e. 100 mM GB spray caused a greater accumulation of GB in the leaves of maize plants than did 0 or 50 mM. Although exogenously applied GB enhanced photosynthetic capacity of salt-stressed plants of both cultivars, it enhanced the activities of antioxidant enzymes, SOD, CAT and POD, in salt-stressed plants of cv. C-20 only. Overall, the adverse effects of salt stress on maize plants were alleviated by the exogenous application of GB at different growth stages, which up-regulated photosynthetic capacity and the activities of some antioxidant enzymes.
Spat Collection and Experimental Culture of the Atlantic Pearl Oyster, Pinctada imbricata (Bivalvia: Pteriidae), under Suspended Conditions in the Caribbean

Several suspended culture experiments of the Atlantic Pearl oyster, Pinctada imbricata, were carried out in the Colombian Caribbean. Seed was collected at two seasons of the year (rainy and dry) using onion bags collectors. Shell growth and survival of the oysters, as well as frequency and size of predators, were evaluated under different stocking densities (10, 20, 30, and 40% coverage of the bottom of the net), culture systems (pearl nets and pocket nets), seasons (rainy and dry), and depths (4 and 9 m). A greater amount of P. imbricata spat was collected during the dry season (297 ind./m2) than in the rainy season (72 ind./m2). Density influenced significantly the growth of P. imbricata but not its survival. The highest antero-posterior measure (APM) values appeared at lower densities (10 and 20% bottom coverage). Growth and survival values of the pearl oysters were greater in pocket nets than in pearl nets. Culture season did not affect the survival of the oysters but their growth was greater in the dry season than in the rainy season. Culture depth influenced oyster growth but not their survival, with recorded shell APM being greater at 9 m than at 4 m. The following predators: Cymatiidae, Portunidae, Majidae, and Xanthidae were found inside the culture nets. Highest mortality values of the pearl oysters coincided with high frequency and/or size of predators. Frequency of cymatids was higher during the rainy season and in pearl nets with high oyster density. Xantids appeared mostly in pocket nets and majids during the dry season.
Performance of circle hooks in a mixed‐species recreational fishery

Summary The effects of the circle-hook on hooking injury, fish size and catch rates were evaluated for mixed-species recreational boat fisheries in the Balearic Islands (NW Mediterranean). The relationships between deep-hooking incidence (the main cause of fish mortality) and hook type (circle hooks vs conventional J-hooks), fish size, and angler were tested. Results demonstrated that the size of the fish was the most important predictor of deep-hooking incidence, as a larger fish size resulted in an increase in deeper wounds. Hook type also had a significant effect on the deep-hooking rate. Specifically, the deep-hooking rate of the most frequently-caught species, Diplodus annularis (L.) and Coris julis (L.), was reduced by greater than half with the circle hooks. The mean size of the fish caught and the unhooking time were not affected by the use of circle hooks. Finally, their use resulted in no change in the catch per unit effort (CPUE) compared to conventional hooks for both univariate and multivariate procedures. Hence, circle hooks are a promising conservation tool for marine resources when linked with catch-and-release strategies.
Somatic musculature of Tardigrada: phylogenetic signal and metameric patterns

Although studies describing molecular-based phylogenies within tardigrades are now frequently being published, this is not the case for studies combining molecular and morphological characters. Tardigrade phylogeny is still based, from a morphological point of view, almost exclusively on chitinous structures and little attention has been given to detecting and using novel morphological data. Consequently, we analysed the musculature of seven tardigrade species belonging to the main phyletic lines by confocal laser scanning microscopy and compared these morphological results with new molecular analyses (18S+28S rRNA genes). Finally, we analysed all the data with a total evidence approach. A consilience in the phylogenetic relationships among orders and superfamilies of tardigrades was obtained among the evolutionary trees obtained from morphological, molecular and total evidence approaches. Comparative analysis on the musculature allowed the identification of serial homologies and repeated metameric patterns along the longitudinal animal body axis. A phenomenon of mosaic evolution was detected in musculature anatomy, as dorsal musculature was found to be highly modified with respect to the other body muscle groups, probably related to the evolution of dorsal cuticular plates. An understanding of tardigrade musculature anatomy will give fundamental information to understand the evolution of segmental pattern within Panarthropoda. © 2013 The Linnean Society of London
Heterosis and combining ability for fruit yield and its component traits in bottle gourd [Lagenaria siceraria (Mol.) Standl.]

Heterosis studies with 50 hybrids generated through line x tester (10 lines and 5 testers) analysis were carried out to estimate the extent of heterosis over better parents and standard check (NDBG-104) for fruit yield and its contributing traits in bottle gourd. The range of heterosis observed in number of crosses exhibiting significantly negative and positive heterosis over better parent and standard check showed considerable differences from Kharif season to summer season. The results revealed that cross Punjab Long x Thar Samruddhi in E1, Punjab Long x PSPL in E2 and Arka Bahar x Punjab Komal in E3 exhibited significant positive heterosis over better parent for fruit yield per plant. For standard heterosis, the cross ABG-1 x NDBG-517 in E1, NDBG-104 x Thar Samruddhi in E2 and Pusa Naveen x PSPL in E3 had significant and high heterotic effects in positive directions. The crosses which had larger estimates of heterobeltiosis and standard heterosis for fruit yield per plant, also exerted significant positive heterotic effects for fruits per plant, fruit weight and main vine length in atleast two environments. These cross combinations can be exploited in breeding programmes in bottle gourd.
PENGARUH KOLKISIN TERHADAP PERTUMBUHAN, UKURAN SEL METAFASE DAN KANDUNGAN PROTEIN BIJI TANAMAN KACANG HIJAU ( Vigna radiata (L) Wilczek)

T he aims of this experiment was to know of colchicine on growth, metaphase cell size and improvement seeds protein. The experiment includes one factor is colchicines consentration 0% (K0), 0,05% (K1), 0,l0% (K2), 0,15% (K3), and 0,20% (K4) with 5 replicates. The research use Complete Randomized Design, when there is a real difference Duncan test will be used upon significant degree of 5%. The observed parameters are sum of leafe, wet weight and dry weight plant, metaphase cell size and content protein seeds. The result of research indicated that colchicines growth hampered, increased of metaphase cell size and improvement protein seeds.
Effects of elevated CO2 and transgenic Bt cotton on plant chemistry, performance, and feeding of an insect herbivore, the cotton bollworm

Effects of elevated atmospheric CO2 (double‐ambient CO2) on the growth and metabolism of cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), fed on transgenic Bacillus thuringiensis (Berliner) (Bt) cotton [Cry1A(c)], grown in open‐top chambers, were studied. Two levels of CO2 (ambient and double‐ambient) and two cotton cultivars (non‐transgenic Simian‐3 and transgenic GK‐12) were deployed in a completely randomized design with four treatment combinations, and the cotton bollworm was reared on each treatment simultaneously. Plants of both cotton cultivars had lower nitrogen and higher total non‐structural carbohydrates (TNC), TNC:Nitrogen ratio, condensed tannin, and gossypol under elevated CO2. Elevated CO2 further resulted in a significant decrease in Bt toxin level in GK‐12. The changes in chemical components in the host plants due to increased CO2 significantly affected the growth parameters of H. armigera. Both transgenic Bt cotton and elevated CO2 resulted in a reduced body mass, lower fecundity, decreased relative growth rate (RGR), and decreased mean relative growth rate in the bollworms. Larval life‐span was significantly longer for H. armigera fed transgenic Bt cotton. Significantly reduced larval, pupal, and adult moth weights were observed in the bollworms fed elevated CO2‐grown transgenic Bt cotton compared with those of bollworms reared on non‐transgenic cotton, regardless of the CO2 level. The efficiency of conversion of ingested food and of digested food of the bollworm were significantly reduced when fed transgenic Bt cotton, but there was no significant CO2 or CO2× cotton cultivar interaction. Approximate digestibility of larvae reared on transgenic cotton grown in elevated CO2 was higher compared to that of larvae fed non‐transgenic cotton grown at ambient CO2. The damage inflicted by cotton bollworm on cotton, regardless of the presence or absence of insecticidal genes, is predicted to be more serious under elevated CO2 conditions because of individual compensatory feeding on host plants caused by nitrogen deficiency.
Interactive Cohort Identification of Sleep Disorder Patients Using Natural Language Processing and i2b2

Summary Nationwide Children’s Hospital established an i2b2 (Informatics for Integrating Biology & the Bedside) application for sleep disorder cohort identification. Discrete data were gleaned from semi-structured sleep study reports. The system showed to work more efficiently than the traditional manual chart review method, and it also enabled searching capabilities that were previously not possible. Objective: We report on the development and implementation of the sleep disorder i2b2 cohort identification system using natural language processing of semi-structured documents. Methods: We developed a natural language processing approach to automatically parse concepts and their values from semi-structured sleep study documents. Two parsers were developed: a regular expression parser for extracting numeric concepts and a NLP based tree parser for extracting textual concepts. Concepts were further organized into i2b2 ontologies based on document structures and in-domain knowledge. Results: 26,550 concepts were extracted with 99% being textual concepts. 1.01 million facts were extracted from sleep study documents such as demographic information, sleep study lab results, medications, procedures, diagnoses, among others. The average accuracy of terminology parsing was over 83% when comparing against those by experts. The system is capable of capturing both standard and non-standard terminologies. The time for cohort identification has been reduced significantly from a few weeks to a few seconds. Conclusion: Natural language processing was shown to be powerful for quickly converting large amount of semi-structured or unstructured clinical data into discrete concepts, which in combination of intuitive domain specific ontologies, allows fast and effective interactive cohort identification through the i2b2 platform for research and clinical use. Citation: Chen W, Kowatch R, Lin S, Splaingard M, Huang Y. Interactive cohort identification of sleep disorder patients using natural language processing and i2b2. Appl Clin Inf 2015; 6: 345–363 http://dx.doi. org/10.4338/ACI-2014-11-RA-0106
MICROBIOLOGICAL AND NUTRITIONAL QUALITY OF SOUS VIDE OR TRADITIONALLY PROCESSED FISH: INFLUENCE OF FAT CONTENT

Sous vide technology, which implies vacuum packaging, pasteurization and storage at a chill temperature, also implies a challenge to obtain a balance between an extended shelf life and microbiological safety of the product with a fresh-like appearance and a high nutritional value. The aim of this work was to determine the effect of fat content of two fish species (salmon and trout) on survival of mesophilic, psychrotrophic, Enterobacteriaceae, Micrococaceae and anaerobes during sous vide cooking and their control during chilled storage for 3, 20 and 45 days. Proximate and fatty acid composition was also determined using a traditional cooking method. Analyses were undertaken comparatively with the same culinary technique for sous vide and traditionally cooked fish. Higher fat levels in all products resulted in fewer microbial reductions. In order to establish adequate pasteurization (temperature/time) combinations for the assurance of microbial quality and shelf life of sous vide processed fish, fat content and fatty acid composition of food should be considered.
Inbreeding Effects on Layer Performance at Two Levels of Protein Intake

Abstract The growth and laying performance of two strains (6 and 8) after two generations of close inbreeding (44%) were compared with that of their reciprocal crosses (6 × 8 and 8 × 6) under two levels (17% and 13%) of dietary protein and a corresponding 19% reduction in daily protein intake. Inbreeding effects were large for most traits measured, including body weights, components of egg production, and egg quality. Reduced dietary protein had adverse effects upon viability, egg mass, age at first egg, and body weight late in the laying period. However, this dietary modification improved egg mass produced per kilogram of protein intake and had no effects upon egg quality. The tendency for inbreeding depression to be greater under the low (13%) protein diet for viability, sexual maturity, and egg production was too slight for statistical significance. Inbreeding did not change feed or protein conversion response to low protein because inbreds increased their intake of the low protein diet less than the crosses.