Miracle-dz/UniFAR-FTUs huggingface dataset:
Fluorogenic properties of 4-dimethylaminocinnamaldehyde (DMACA) enable high resolution imaging of cell-wall-bound proanthocyanidins in plant root tissues

Proanthocyanidins (PAs) are polymeric phenolic compounds found in plants and used in many industrial applications. Despite strong evidence of herbivore and pathogen resistance-related properties of PAs, their in planta function is not fully understood. Determining the location and dynamics of PAs in plant tissues and cellular compartments is crucial to understand their mode of action. Such an approach requires microscopic localization with fluorescent dyes that specifically bind to PAs. Such dyes have hitherto been lacking. Here, we show that 4-dimethylaminocinnamaldehyde (DMACA) can be used as a PA-specific fluorescent dye that allows localization of PAs at high resolution in cell walls and inside cells using confocal microscopy, revealing features of previously unreported wall-bound PAs. We demonstrate several novel usages of DMACA as a fluorophore by taking advantage of its double staining compatibility with other fluorescent dyes. We illustrate the use of the dye alone and its co-localization with cell wall polymers in different Populus root tissues. The easy-to-use fluorescent staining method, together with its high photostability and compatibility with other fluorogenic dyes, makes DMACA a valuable tool for uncovering the biological function of PAs at a cellular level in plant tissues. DMACA can also be used in other plant tissues than roots, however care needs to be taken when tissues contain compounds that autofluoresce in the red spectral region which can be confounded with the PA-specific DMACA signal.
The impact of exposure to HPV related information and injunctive norms on young women's intentions to receive the HPV vaccine in China: A structural equation model based on KAP theory

Background The HPV vaccination is a crucial line of defensing against cervical cancer. As a result of government support and positive publicity from the majority of media, a craze for HPV vaccination has occurred in China. Besides, the intentions to get the HPV vaccine among women of appropriate age is also influenced by families' and friends' attitudes and perceptions toward HPV vaccine. Therefore, the purpose of this study was to investigate how HPV related information exposure and injunctive norms affect young Chinese women's intentions to receive the HPV vaccine. Methods A structural equation model was developed based on KAP theory, and 567 effective questionnaires were collected through an online survey. We used SPSS 26.0 for the reliability and validity analysis and the differential testing of demographic characteristics, and Amos 26.0 for the goodness-of-fit analysis and paths testing of the model. Results Our findings showed that (1) intention to receive HPV vaccine differed significantly in age (P = 0.046), educational background (P = 0.001), and occupation (P = 0.004). (2) Exposure to HPV related information positively affected knowledge about HPV (β = 0.316, P < 0.001) and intention to receive HPV vaccine (β = 0.141, P < 0.001). (3) Knowledge about HPV positively affected attitude toward HPV vaccine (β=0.341, P < 0.001), but negatively affected intention to receive HPV vaccine (β = −0.148, P < 0.05), and attitude toward HPV vaccine positively affected intention to receive HPV vaccine (β = 0.594, P < 0.001). (4) Injunctive norms positively affected attitude toward HPV vaccine (β = 0.362, P < 0.001) and intention to receive HPV vaccine (β = 0.420, P < 0.001). Conclusions Exposure to HPV related information influenced young Chinese women's intentions to receive the HPV vaccine and related knowledge, that is, the more frequently they were exposed to HPV related information, the stronger their intentions to receive the vaccine and the higher their HPV knowledge. Also, the perception and support of HPV vaccination by people around them will further influence their attitudes and intentions to receive the HPV vaccine.
Effect of a novel dietary supplement Khejri, and Spirulina supplementation on lipid profile in cricket players

Prosopis cineraria (Fabaceae) is known as Khejri in India or the golden tree of Indian deserts. It’s potential as a dietary supplement in sports nutrition and its effect on regulating lipid profile has never been investigated. Spirulina (Arthrospira platensis) is a superfood with high nutritional value and is a popular supplement among athletes. In the current study, Spirulina and Khejri were used as supplements by cricket players to improve their physical fitness and lipid profile. Both supplements were given to individual groups and in combination to see the combined effect. The intervention period was 21 days, and supplements were given in 500 mg doses daily. Lipid profile assessments were done before and after the intervention period. 40 cricket players were divided into 4 groups: Group 1 (n = 10): Both supplements, Spirulina and Khejri, Group 2 (n = 10): Supplement Spirulina, Group 3 (n = 10): Supplement Khejri, and Group 4 (n = 10): Control. When experimental groups 1, 2 and 3 were compared to the control group 4, significant reduction was observed in triglyceride levels (Group1 vs. control: 141.53 ± 14.74 vs. 199.28 ± 27.24, p < 0.05; Group 2 vs. control: 137.5 ± 14 vs. 199.28 ± 27.24, p < 0.05; Group 3 vs. control: 135.32 ± 17.34 vs. 199.28 ± 27.24, p < 0.05) and significant reduction in cholesterol levels was found post-intervention after 21 days of supplementation (Group1 vs. control: 149.75 ± 7.08 vs. 207.86 ± 11.69, p < 0.001; Group 2 vs. control: 178.28 ± 9.43 vs. 207.86 ± 11.69, p < 0.05; Group 3 vs. control: 142.92 ± 10.01 vs. 207.86 ± 11.69, p < 0.001). Cholesterol and Triglyceride levels were significantly decreased pre- vs. post-intervention by Khejri and Spirulina supplements in cricket players.
Using acoustic velocities and microimaging to probe microstructural changes caused by thermal shocking of tight rocks

Introduction: Large scale, Earth processes and bulk rock properties are influenced by underpinning, dynamic, microstructures within rocks and geomaterials. Traditionally, the amount of porosity has been considered the primary control on important bulk rock properties like seismic wave velocities (Vp and Vs) and permeability. However, in tight rocks, velocity and permeability (k) can change substantially despite small changes in the amount of porosity during cracking. Therefore, other microstructural features inherent to given lithologies, such as heterogeneity and anisotropy in mineral properties are considered as factors controlling these bulk rock properties. Understanding which microstructural features control Vp, Vs, and permeability in tight rocks is useful in applications like enhanced geothermal systems (EGS), where thermal shocking is used to increase permeability. Thermal shocking involves injecting surface water into the subsurface to cool mineral crystals, induce contraction of crystals, and cause thermal cracking. Methods: We tested three tight lithologies with unique microstructures; granodiorite (SWG), basalt (PTB), and carbonate (MSA). We simulated thermal shocking by slowly heating samples to 350°C and then quenching them. We chose a temperature of 350°C because thermal shocking at this temperature is not well documented in literature, and this temperature is relevant to EGS. Using time-lapse microimaging, we assessed how thermal cracking occurs in each lithology and explored how thermal cracks influence connected porosity, Vp, Vs, and k. Results: Microimaging shows extensive cracking in the SWG and MSA lithologies, and little to no cracking in PTB with thermal shocking treatment. Vp and Vs became more pressure sensitive, and elastic moduli decreased with treatment for all lithologies. This may be caused by reduced stiffness between mineral crystal boundaries with treatment. Discussion: Lithologies with minerals that have anisotropy of or a wide range in thermal conductivity and/or thermal expansion coefficients can have increased thermal cracking. In thermally shocked SWG and MSA, Vp and Vs are good indicators of thermal cracking and k increases, but less so in PTB. Lithologies like PTB may require multiple thermal shock stimulations to increase permeability. Our results highlight how micro-scale changes influence bulk rock properties and when we can monitor permeability increases and microscale thermal cracking with Vp and Vs.
Exploring the causality and pathogenesis of systemic lupus erythematosus in breast cancer based on Mendelian randomization and transcriptome data analyses

Introduction There has been a cumulative interest in relationships between systemic lupus erythematosus (SLE) and cancer risk. Breast cancer is the most common cancer among women worldwide. However, the casual association and pathogenesis between SLE and breast cancer remains incompletely unknown. Methods Mendelian randomization (MR) analysis was first conducted to investigate the potential causality between SLE and breast cancer. Sensitivity analyses were applied to validate the reliability of MR results. Transcriptomic data analyses based on the Cancer Genome Atlas and Gene Expression Omnibus databases were then performed to identify and construct a SLE-related gene signature (SLEscore). Results The MR analysis demonstrated that genetic predisposition to SLE was casually associated with the decreased risk of breast cancer in the East Asian cohort (odds ratios: 0.95, 95% confidence interval: 0.92-0.98, p=0.006). However, no casual associations were observed in the European population. Furthermore, sensitivity analyses proved the robustness of the present MR results. A prognostic SLEscore consisting of five SLE-related genes (RACGAP1, HMMR, TTK, TOP2A, and KIF15) could distribute patients with breast cancer into the high- and low-risk groups according to survival rates with good predictive ability (p < 0.05). Conclusion Our MR study provided evidence that genetic changes in SLE were significantly associated with the decreased risk of breast cancer in the East Asian population, while no causality was found in the European cohorts. Transcriptome data analyses indicated that the SLEscore could serve as a novel biomarker for predicting prognosis when breast cancer and SLE coexisted in patients.
Numerical simulation of mechanical compaction and pore evolution of sandstone considering particle breakage

Mechanical compaction is an important diagenetic process in sandstone reservoirs. Particle breakage, which commonly occurs during mechanical compaction, plays a significant role in controlling the physical properties of the reservoir. However, existing numerical simulation methods have limitations in simulating mechanical compaction when considering particle breakage. In this study, a discrete element simulation method of mechanical compaction was proposed based on particle cutting, and the experimental results reported in the literature were used to calibrate the simulation parameters. Finally, this method was applied to the simulation of the mechanical compaction of sandstone to analyze the pore evolution process. The results show that the new simulation method has high computational efficiency and can reflect the process of particle breakage. The simulation results coincide well with the experimental results. In the simulated mechanical compacted process of coarse sandstone, particle breakage is strong in the high-stress stage with a vertical stress of 30 MPa–50 MPa. The porosity and mean radii of pores and throats decreased rapidly, and the number of pores and throats increased rapidly in the high-stress stage. When the vertical stress reached 50 MPa, compared to the simulation results without considering particle breakage, the porosity difference rate caused by particle breakage was 4.63%; the radius difference rates of pores and throats were 2.78% and 6.8%, and the number difference rates of pores and throats were 4.95% and 8.74%, respectively. In the process of mechanical compaction, the pore evolution of the reservoir is controlled by the filling of the pre-existing pore space by the fragments generated through particle breakage and the generation of microfractures. Additionally, the simulation method presented in this study can be applied to complex geological conditions and can be combined with other reservoir simulation methods. The simulation results can provide rich training samples for artificial intelligence and other emerging technologies.
The more academic burnout students got, the more problematic mobile phone use they suffered? A meta-analysis of mainland Chinese adolescents and young adults

Introduction In recent years, the relationship between academic burnout (AB) and problematic mobile phone use (PMPU) has become the hot issue of scholars, and a lot of related research has been carried out, but the results are different. Most studies showed that there was a significant positive correlation between AB and PMPU. However, some studies showed that the relationship between AB and PMPU was not significant. While this study aimed at exploring the relationship between AB and PMPU, as well as the influence of some moderating factors (region, age, gender, publication year, the measurement instrument) on them. Methods According to the searching process for studies of PRISMA, we searched the related studies on AB and PMPU in Mainland China from January 2012 to November 2022 from CNKI, Wanfang Data, Chongqing VIP Information Co., Ltd. (VIP), Baidu scholar, ProQuest dissertations, Taylor & Francis, Springer, Web of Science, Google Scholar, EBSCO and PsyclNFO. Eventually, 50 studies were included in the meta-analysis, involving 38,488 subjects, with the sample size ranging from 193 to 2,260. CMA 3.0 was used to analyze the overall effect and test the moderating effect. Results The result shows that the relationship between AB and PMPU can be considered as a moderate correlation (r = 0.414, 95%CI [0.384, 0.443]), and moderator analysis shows that the relationship between AB and PMPU was moderated by the measurement instrument and publication year. Discussion Specifically, when the Mobile Phone Addiction Tendency Scale and the Smartphone Addiction Scale for College Students were used as PMPU measurement tools, the correlation coefficients between AB and PMPU were higher. When the publication year was used as a moderating variable, the relationship between AB and PMPU increased over the years. Systematic Review Registration: https://www.crd. york.ac. uk/prospero/display_record. php? ID=CRD42022347277, identifier PROSPERO CRD42022347277.
Charge Transport and Storage in Metal‐Nitride‐Oxide‐Silicon (MNOS) Structures

A simple physical model that predicts charge accumulation at the dielectric interface of metal‐nitride‐oxide‐silicon (MNOS) structures is proposed and verified experimentally. The model is based on the presence of steady‐state current flow in the dielectric structure. Interface‐charge accumulation is shown to be determined by the requirement for continuity of current through the structure under steady‐state conditions. Continuity of current is established by accumulation of either positive or negative charge for a given polarity of charging voltage, depending on the relative current‐field characteristics of the silicon nitride and silicon dioxide layers. Due to the exponential nature of the current‐field characteristics, the time required to reach steady state is a strong function of the applied charging voltage. This leads to the observed charge storage property of MNOS devices. The hysteresis characteristic observed in MNOS structures is shown to be time‐dependent with a tendency to merge into a single‐.
Efficacy on gait and posture control after botulinum toxin A injection for lower-limb spasticity treatment after stroke: A randomized controlled trial

Objectives To observe the efficacy of botulinum toxin type A (BoNT-A) for the spasticity of the lower-limb post-stroke on gait and posture control. Methods A total of 46 patients with hemiplegia gait were randomly divided into the experimental group (23 patients) and the control group (23 patients). In patients in the experimental group received injections of BoNT-A by electrical stimulation-guided. At the same time, patients of the two groups received routine physical therapy. Gait analysis, plantar pressure analysis, lower-limb Fugl–Meyer assessment (L-FMA), 10 meter walking test (10MWT), timed “Up and Go” test (TUGT), and modified Ashworth Scale assess (MAS) of the lower limbs were performed at 0, 1, 4, and 12 weeks after treatment. Results At 1, 4, and 12 weeks after treatment, the L-FMA, stride length, speed, and TUGT significantly improved than 0 week in both groups. The L-FMA and peak of forefoot pressure, and MAS results in the experimental group were better than those in the control group at 4 and 12 weeks. The TUGT, speed, and stride length in experimental group was significantly shortened than that in control group at 1, 4, and 12 weeks. Conclusion Botulinum toxin type A injection can improve motor functions of the lower limb, gait, spasticity, forefoot pressure, and posture control of patients after stroke.
Chronic exposure to low-level lipopolysaccharide dampens influenza-mediated inflammatory response via A20 and PPAR network

Influenza A virus (IAV) infection leads to severe inflammation, and while epithelial-driven inflammatory responses occur via activation of NF-κB, the factors that modulate inflammation, particularly the negative regulators are less well-defined. In this study we show that A20 is a crucial molecular switch that dampens IAV-induced inflammatory responses. Chronic exposure to low-dose LPS environment can restrict this excessive inflammation. The mechanisms that this environment provides to suppress inflammation remain elusive. Here, our evidences show that chronic exposure to low-dose LPS suppressed IAV infection or LPS stimulation-induced inflammation in vitro and in vivo. Chronic low-dose LPS environment increases A20 expression, which in turn positively regulates PPAR-α and -γ, thus dampens the NF-κB signaling pathway and NLRP3 inflammasome activation. Knockout of A20 abolished the inhibitory effect on inflammation. Thus, A20 and its induced PPAR-α and -γ play a key role in suppressing excessive inflammatory responses in the chronic low-dose LPS environment.
Evaluating the effects of two different kinesiology taping techniques on shoulder pain and function in patients with hypermobile Ehlers-Danlos syndrome

Background Ehlers-Danlos Syndrome (EDS) is a group of inherited connective tissue disorders which predominantly affects women and has a prevalence as high as 1 in 5,000 individuals. Hypermobile EDS (hEDS) is the most common subtype of EDS and is characterized by multi-joint pain, particularly in large joints such as the shoulder. Physical therapy is often utilized to address the pain, physical impairments, and functional loss in patients with EDS. Kinesiology Tape (KT) is an intervention commonly used by physical therapists for treating shoulder pain and dysfunction. Studies related to the effectiveness of KT in patients with shoulder pain is equivocal and there are a lack of studies specifically studying the effects of KT in an EDS population. Purpose The purpose of this study was to assess the efficacy and short-term effects of two different KT techniques on shoulder pain and function in individuals with hEDS and shoulder pain. Methods Participants were recruited from EDS support groups in the New England area of the United States; were diagnosed with hEDS by their physician; and had shoulder pain. Baseline demographic information was obtained for each participant followed by completion of 4 patient reported outcome (PRO) measures: the Upper Extremity Functional Index, QuickDASH (Disabilities of the Arm, Shoulder, & Hand), Shoulder Pain and Disability Index, and the Western Ontario Shoulder Instability Index. Current pain level, average pain over the past 24 h, and worst pain over the past 24 h were recorded using the numeric pain rating scale (NPRS). Subjects were randomly assigned to receive either an experimental shoulder KT procedure or a control shoulder taping. Immediately after taping, the NPRS was reassessed. Subjects then returned 48 h later to repeat the NPRS and PRO measures. Results There was no significant difference between the experimental and control tape groups for any outcome measure. There was a significant improvement from pre-taping to 48-hours post taping for each of the 4 PRO measures with large effect sizes (p < 0.001; ƞp2 = .517–.719). Likewise, average, and worst pain over the last 24 h significantly improved with large effect sizes over the same period (p = 0.005; ƞp2 = .225 and p < 0.001; ƞp2 = .382, respectively). Current NPRS levels significantly improved from pre-tape to immediately post-tape (p = .023, ƞp2 = .131) and was maintained through the 48-hour follow up, although no further improvement was seen. Conclusion KT is an inexpensive and relatively safe intervention that is easy to apply and can offer temporary improvements in pain and function for patients with EDS and shoulder pain.
Population pharmacokinetic analysis of TQ-B3203 following intravenous administration of TQ-B3203 liposome injection in Chinese patients with advanced solid tumors

Background: TQ-B3203 is a novel topoisomerase I inhibitor currently in development for the treatment of advanced solid tumors. Great differences in pharmacokinetic characteristics were found among individuals according to the phase I clinical trial following intravenous administration of TQ-B3203 liposome injection (TLI) in Chinese patients with advanced solid tumors. Thus, it is significant to establish a population pharmacokinetic model to find the key factors and recognize their effect on pharmacokinetic parameters in order to guide individualized administration. Methods: Non-linear mixed effect models were developed using the plasma concentrations obtained from the phase I clinical trial by implementing the Phoenix NLME program. Covariates that may be related to pharmacokinetics were screened using stepwise methods. The final model was validated by goodness-of-fit plots, visual predictive check, non-parametric bootstrap and a test of normalized prediction distribution errors. Results: A three-compartment model with first-order elimination was selected as the best structural model to describe TQ-B3203 disposition adequately. Direct bilirubin (DBIL) and body mass index (BMI) were the two most influential factors on clearance, while lean body weight (LBW) was considered to affect the apparent distribution volume of the central compartment. The population estimations of clearance and central volume were typical at 3.97 L/h and 4.81 L, respectively. Model-based simulations indicated that LBW had a great impact on Cmax, BMI exerted a considerable influence on AUC0-t, and the significance of DBIL on both AUC0-t and Cmax was similarly excellent. Conclusion: The first robust population pharmacokinetic model of TQ-B3203 was successfully generated following intravenous administration of TLI in Chinese patients with advanced solid tumors. BMI, LBW and DBIL were significant covariates that affected the pharmacokinetics of TQ-B3203. This model could provide references for the dose regimen in the future study of TLI.