Miracle-dz/UniFAR-FTUs huggingface dataset:
Genomics and Genotyping in Severe Sepsis

The morbidity and mortality of critical illness is still principally determined by the incidence and course of sepsis and its sequelae. Evidence-based efforts to improve the care of sepsis patients include the Surviving Sepsis Campaign and the International Sepsis Forum, and new definitions and diagnostic concepts are being evaluated with the aim of facilitating early diagnosis and evidence-based therapies. Advances include the PIRO (predisposition, infection, response, organ dysfunction) concept, which is designed to classify states of sepsis. PIRO recognizes that the incidence and outcome of sepsis are affected by genetic predisposition, which has been suggested as a tool for risk stratification and even as an inclusion criterion for therapeutic trials. Genomic variants may influence the individual phenotype, including gene expression profiles and protein structure and concentration. Genomics research has therefore entered the area of complex diseases, of which sepsis remains one of the greatest challenges in acute medicine. Genomics and genotyping in the critically ill will be integrated into the field of functional genomics, assisted by high-throughput automated technologies such as microarrays. However, standards and quality-control measures need to be implemented and genotyping in intensive care must be tested in large-scale genetic epidemiological studies. Advances in Sepsis 2004;4(1):11‐6.
Effect of Computer-based Clinical Reminder System to Colorectal Cancer Screening

This paper is focused on the effect of computer-based clinical reminder system to colorectal cancer screening. The reminder system with five DB modules was developed. The paper investigated a total of 541 patients who had been visited health examination center in two hospitals. In this work, 62.1-96.0% was satisfied with the reminder system. The present research showed that a reminder effort of computerized system in mailing was significantly effective for colorectal cancer screening. In order to maintain the reminder effect, computerbased clinical system should be supported by policy maker and need for monitoring the feedback from stakeholders.
Impairments in sensory-motor gating and information processing in a mouse model of Ehmt1 haploinsufficiency

Regulators of chromatin dynamics and transcription are increasingly implicated in the aetiology of neurodevelopmental disorders. Haploinsufficiency of EHMT1, encoding a histone methyltransferase, is associated with several neurodevelopmental disorders, including Kleefstra syndrome, developmental delay and autism spectrum disorder. Using a mouse model of Ehmt1 haploinsufficiency (Ehmt1D6Cre/+), we examined a number of brain and behavioural endophenotypes of relevance to neurodevelopmental disorders. Specifically, we show that Ehmt1D6Cre/+ mice have deficits in information processing, evidenced by abnormal sensory-motor gating, a complete absence of object recognition memory, and a reduced magnitude of auditory evoked potentials in both paired-pulse inhibition and mismatch negativity. The electrophysiological experiments show that differences in magnitude response to auditory stimulus were associated with marked reductions in total and evoked beta- and gamma-band oscillatory activity, as well as significant reductions in phase synchronisation. The pattern of electrophysiological deficits in Ehmt1D6Cre/+ matches those seen in control mice following administration of the selective NMDA-R antagonist, ketamine. This, coupled with reduction of Grin1 mRNA expression in Ehmt1D6Cre/+ hippocampus, suggests that Ehmt1 haploinsufficiency may lead to disruption in NMDA-R. Taken together, these data indicate that reduced Ehmt1 dosage during forebrain development leads to abnormal circuitry formation, which in turn results in profound information processing deficits. Such information processing deficits are likely paramount to our understanding of the cognitive and neurological dysfunctions shared across the neurodevelopmental disorders associated with EHMT1 haploinsufficiency.
Treatment of Uterine Sarcoma with rAd-p53 (Gendicine) Followed by Chemotherapy: Clinical Study of TP53 Gene Therapy.

This study evaluated the efficacy of rAd-p53 (Gendicine®) followed by chemotherapy for the treatment of uterine sarcoma. Twelve cases of uterine sarcoma treated at Shengjing Hospital were retrospectively analyzed. Among the 12 patients, one had primary cancer, and 11 had recurrent cancer. For the recurrent cases, the interval between the first operation and diagnosis of recurrence, or progression-free survival time 1 (PFS1), was 1-18 months (median 3 months). All patients were treated with local application of rAd-p53 followed by chemotherapy (local injection of bleomycin and i.v. infusion of cisplatin, epirubicin, and isocyclophosphamide). Efficacy was evaluated, and the rates of complete remission (CR) and partial remission (PR) were calculated. During follow-up, PFS time 2 (PFS2) after the baseline period and overall survival (OS) time after the baseline period of rAd-p53 treatment data were obtained. The treatment resulted in one CR, seven PR, three with stable disease (SD), and one with progressive disease (PD). The remission rate (CR + PR) was 66.7%, and the responsive (CR + PR + SD) rate was 91.7%. PFS2 ranged from 2 to 62 months, with a median of 13 months, which is 10 months longer than that of PFS1; this difference was statistically significant (p = 0.0038). The OS time ranged from 6 to 62 months, with a median of 24 months. Following the combined treatment, four of the patients underwent a second debulking surgery. Of the two patients with liver metastases, one had CR of liver foci, and one had PR. Up to the follow-up date of the two patients who survived, one was tumor-free for 60 months. The PFS2 for the other patient was 39 months. This patient survived with tumor for 53 months with slow disease progression. The remaining 10 patients died. Local application of rAd-p53 combined with local injection of bleomycin and intravenous infusion of cisplatin, epirubicin and isocyclophosphamide was effective for treatment of uterine sarcoma, especially for patients with liver metastases. For patients with uterine sarcoma who do not have the opportunity for surgery, this regimen can be used as a new adjuvant therapy to obtain a surgical opportunity that allows further debulking of the tumor mass.
GERMINAÇÃO IN VITRO DE EMBRIÕES ZIGÓTICOS DE MURMURU (Astrocaryum ulei) In vitro germination of Murmuru zygotic embryos (Astrocaryum ulei)

The work aimed to evaluate the influence of sucrose concentrations and seed physiologic age on the in vitro germination of murmuru zygotic embryos. Fruits collected from field plants in two development stages had the embryos excised, desinfected and inoculated onto MS medium with 75% salts, 2.5 g.L -1 of giberelic acid and different sucrose concentrations: 15, 30 and 45 g.L -1 . All cultures were kept for 30 days in the dark at 25±2oC, being transferred afterwards to a growth chamber with 30 mmol.m -2 s -1 of radiation and 16 hours photoperiod for complete development. The statistical design was a 2x3 factorial, with six repetitions and five embryos by experimental unit. After 30 days it was evaluated the germination percentage and plantlets height. It was verified that embryos from immature fruits presented higher germination percentage and the sucrose concentration of 30 g.L -1 was the one that provided the best results among the others tested. Embryos from mature fruits gave rise to plantlets with average height significantly greater than that of plantlets from immature fruits and 30 g.L -1 of sucrose was the concentration that provided the best results.
Effect of rootstock and time of grafting in low-chill peach cultivars

An experiment was conducted to study the propagation of low-chill peaches in Tarai region of Uttarakhand. Three cultivars of peach viz. Pratap, Shan-i-Punjab and Saharanpur Prabhat were grafted at seven days intervals on two different rootstocks. Grafting at third week of February recorded the earliest sprouting, more success (bud take percentage) and maximum number of saleable plants than other grafting times which was started from second week of January to last week of February. Wild peach can be used as rootstocks for low-chill peaches to obtain more successful plants over plum rootstocks. Low-chill peach cv. Pratap, resulted the highest sprouting percentage as well as saleable plants percentage followed by Shan-i-Punjab and Saharanpur Prabhat.
The Co-Morbidities and Mortality Rate among Rheumatoid Arthritis Patients at the Western Region of Saudi Arabia A Retrospective Cross-Sectional Study

To evaluate the mortality rate in a well defined hospital; based patients diagnosed with rheumatoid arthritis over a three year period and to investigate the causes of death with their associated risk factors. A retrospective cross-sectional study was conducted for rheumatoid arthritis patients over a three year period (2006-2008) at King Abdulaziz University Hospital in Jeddah at the western region of Saudi Arabia. The causes of death and the associated co-morbidities were registered and compared statistically in relation to the mortality rate. Patients who fulfilled the criteria of the study were included (n = 116). The mortality rate was 16%. The most important causes of death and contributing factors were cardiovascular system diseases and infections (58%), followed by respiratory system diseases (47%) and renal impairment (32%). The most common co- morbidities were diabetes mellitus, hypertension and obesity (p < 0.001, 0.01, 0.03). This study gives important information of the variables associated with the mortality in rheumatoid arthritis and the associated factors which may accelerate the occurrence of an earlier death.
CYP450 Genotype/Phenotype Concordance in Mexican Amerindian Indigenous Populations–Where to from Here for Global Precision Medicine?

Abstract Global precision medicine demands characterization of drug metabolism and phenotype variation in diverse populations, including the indigenous societies. A related question is the extent to which CYP450 drug metabolizing enzyme genotype and phenotype data are concordant and whether they can be used interchangeably. These issues are increasingly debated as precision medicine continues to expand as a popular research topic worldwide. We report here the first study in clinically relevant CYP450 drug metabolism phenotypes and genotypes in Mexican Amerindian indigenous subjects. In a large sample of 450 unrelated and medication free Mexican Amerindian indigenous healthy persons from four Mexican states (Chihuahua, Durango, Nayarit, and Sonora), we performed multiplexed phenotyping for the CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 drug metabolizing enzymes using the CEIBA cocktail and genotyped the same pathways for functional polymorphic variation. Remarkable interindividual variability was found fo.
Hepatoprotective Screening of Polyherbal Extract of Anonna squamosa and Nigella ativa

The present study was carried out to investigate the effects of Polyherbal extract (PHE) on liver fibrosis in rats induced by carbon tetrachloride (CCl4) and to explore its possible mechanisms. Liver injury was induced in Male Wistar albino rats by injection with 50% CCl4 subcutaneously twice a week for 8 weeks. At the same time, Polyherbal extract (50, 100 and 150 mg/kg) was administered intragastrically. Upon pathological examination, the Polyherbal extract-treated rats significantly reduced the liver damage and the symptoms of liver injury. Administration of Polyherbal extract (PHE) decreased CCl4induced elevation of serum transaminase activities, hyaluronic acid, laminin and procollagen type III levels, and contents of hydroxyproline in liver tissue by approximately 30–60%. It also restored the decrease in SOD and GSH-Px activites and inhibited the formation of lipid peroxidative products during CCl4 treatment. Moreover, Polyherbal extract (100, 150 mg/kg, ig) decreased the elevation of TGF-β1 by 47.7% and 53.1%, respectively.These results suggested that Polyherbal extract significantly inhibited the progression of hepatic injury induced by CCl4, and the inhibitory effect of Polyherbal extract on hepatic injury might be associated with its ability to scavenge free radicals, decrease the level of TGF- β1.
The heart failure in Internal Medicine in Tuscany: the SMIT Study

The incidence and prevalence associated with the growing age of population have made heart failure (HF) a new epidemic. In Italy most of HF patients are admitted to Internal Medicine Departments (IMD). We conducted a 30-day cross-sectional study involving patients with HF admitted to IMD of Tuscany. The aim of the study was to provide an updated snapshot of in-hospital management of HF in Tuscany and to analyze the differences in relation to the most recent guidelines. We have recruited 770 patients (M=45.4%) with a mean age of 82.5±8.9 years. Only 16.1% had a de novo HF. Most of them were re-admitted for decompensated HF. Hypertension and ischemic disease are the prevailing etiologies. HF alone was in less than 2%. 71.5% of patients had more than two comorbidities and 40.6% more than three concomitant diseases. The mean hospital stay (overall 8.6±5.5 days) correlated with the number of comorbidity. About 25% of patients had a creatinine clearance <30 mL/min. Nearly 50% of patients had atrial fibrillation but only the half was anti-coagulated. β -blockers, angiotensin converting enzyme-inhibitors (ACE-I) or angiotensin receptor blockers (ARB) were prescribed in about two-thirds of the subjects. Echocardiography was performed in 64.1% of patients. BNP or NT-pro BNP was tested in 67% at the hospital admission and in 18.3% at the discharge. At the discharge 50.1% of patients had loss of autonomy, 57% a polypharmacy prescription (≥8 classes of medicines) and 21.2% needed domiciliary oxygen therapy, but despite these critical points only 8% of the patients were transferred to long-term/intermediate care settings. In conclusion, the main characteristics of patients with HF admitted to IMD in Tuscany are the advanced age (the patients are old and very old) and the presence of multiple comorbidities (HF alone is a rarity indeed ). The use of echocardiography and the pharmacological therapy with ACE-I, ARB, β -blocker and anti-aldosterone agents is wider than previous surveys, but some diagnostic, therapeutic and prognostic aspects are not similar to that recommended by the most recent HF guidelines. This survey underscores again some differences between HF trials world and HF real world , where the management is probably also driven by a clinical holistic approach.
Clinical Profile of patients with Organophosphorus Poisoning in an Intensive Care Unit in a tertiary hospital

Aims - Organophosphorus poisoning is the most common medico toxic emergency in India. Respiratory failure is the most common complication of OP compound leading to death. Early recognition and prompt ventilation may improve survival. The aim of the study was to correlate between the clinical score described by Peradenya Organophosphorus Poisoning (POP) scale, serum pseudocholinesterase level at presentation, ventilator requirement and the outcome. Methods: Patients of OP poisoning attending Victoria Hospital from Jan 2011 to Dec 2011 were studied. Sixty Four patients fulfilled the inclusion criteria. Fifty one of them required ventilator support. Twelve of them succumbed. None of the non ventilated patients died. Clinical scoring by Peradenya scoring and serum pseudocholinesterase level was measured in all patients. Results: The severity of poisoning as measured by POP scale directly correlated with serum cholinesterase level (P<0.001). There were 50% patients in moderate poisoning score and only 5% patients in severe poisoning. A total of 18% of the patients died of which 85% belonged to moderate and severe group. POP scale directly correlated with death outcome (P<0.001). It was also seen that all the patients with pseudocholinesterase level less than 50% of normal range were ventilated. Lower Pseudocholinesterase level also directly correlated to death outcome (P<0.001). Conclusion : The POP scale and serum cholinesterase at presentation appeared useful to assess the severity of poisoning, particularly in terms of need for ventilator and prolonged duration of hospital stay.
Safety evaluation of antitubercular therapy under revisednational tuberculosis control programme in India

Introduction: The World Health Organization declared tuberculosis (TB) as a global emergency in 1993. To intensify the efforts to control TB, the Government of India gradually replaced the National Tuberculosis Programme by the Directly Observed Short Course Therapy (DOTS) programme which is now known as the Revised National Tuberculosis Programme (RNTCP). Objectives: The present study was carried out to evaluate the safety of the DOTS therapy by monitoring adverse drug reactions (ADRs). Methodology: All the TB patients admitted at the DOTS centre Kasturba Hospital, Manipal, and at the DOTS Centre, Udupi, were enrolled as per the study criteria and were monitored for ADRs. The data were evaluated for patient demography, types of TB, types of DOTS treatment, incidence of ADRs, predisposing factors for developing ADRs and the types, onset, management and outcome of the ADRs. ADRs were also assessed for their causality and severity as per the standard algorithms. Results: Out of 94 TB patients, a majority of them were males (70%) and belonged to the age group of 18-40 years (52%). The incidence of ADRs was 17.02%. Gastritis was the most common ADR and multiple drug therapy was the major predisposing factor. We found that 28.51% of the total ADRs belonged to Type-A ADRs. In 87.1% of the cases, the suspected drug was continued in spite of the ADR, without any complications. On evaluation of the causality of ADRs, a majority of them were found to be ‘possible’ by both WHO and Naranjo’s scales. The severity assessment of ADRs showed that 31(51%) reactions were moderate and 30 (49%) were of the ‘mild’ nature. Conclusion: We found DOTS therapy to be safer. But regular monitoring is required for ADRs, so that certain percentage of ADRs can be prevented.
AN ASSESSMENT OF IMPACT OF GLOBALIZATION ON DRESSING STYLE OF UNDERGRADUATE STUDENTS OF BENUE STATE

This study assesses the impact of globalization on dressing style of undergraduate students of Benue State University. The objectives of the study were to highlight the pattern of dressing by the undergraduate students, find out the source of knowledge of the pattern, analyse the impact of the style on the school community and to provide guides towards improving the pattern of dressing. Literature was reviewed on the concept of globalisation and its impact on the culture of developing countries. Open-ended direct interview and questionnaire were used as methods of data collection. The findings of the study indicated that the dressing style of majority of the students was indecent and completely unacceptable to the school community. Majority of the female students wear very tight, short and transparent clothes that expose parts of their bodies considered private. The situation was not different with the male students either. The main sources of their dressing knowledge were internet, Television, magazines and imitation from fellow students. Students have increased access to these sources of dressing information through globalization activities. The impact of this is negative as it was assessed to contributes to distractions for students and staff, sexual harassment and seduction, lack of concentration in academics by students and creation of negative image of the school. The paper recommends for moral lectures to be given to students in every department.
Histological Study of the Oesophagus and Stomach in Grey-Backed Shrike (Lanius tephronotus)

La microestructura del esofago y el estomago del alcaudon gris fueron examinados mediante microscopia de luz. Los resultados mostraron que las glandulas del esofago eran glandulas mucosas, y que las celulas glandulares de descamacion y la mucina de las glandulas del esofago eran secretadas al lumen. El numero de glandulas en la porcion toracica del esofago fue menor que en la porcion cervical. Las aberturas de las glandulas mostraron una reaccion positiva intensa al PAS y azul de Alcian. Se encontraron dos tipos de glandulas en la lamina propia. Las glandulas tubulares simples se localizaron en la lamina superficial de la lamina propia, mientras que las glandulas tubulares compuestas se hallaron en la lamina de profundidad. El epitelio se encontro revestido por una capa cuticular en la zona intermedia entre el proventriculo y la molleja. La superficie de la molleja presento una cuticula gruesa, demostrada por una matriz horizontal positivo para azul de Alcian y varillas verticales positivas para el PAS.
Sources of stress for emergency nurses in four urban Canadian emergency departments.

: The results of this study indicate that emergency nurses experience work-related stress originating from a variety of sources. Although the study included a group of emergency nurses committed to achieving a high standard of patient care, many of the key stressors they confront hinder their sense of achievement. Repeatedly, nurses described inadequate staffing and resources, too many nonnursing tasks, changing trends in ED use, and patient transfer problems as having considerable impact on their ability to provide quality patient care. Nurses were also stressed by continual confrontation with patients and families who exhibited crisis or problematic behavior. In addition to strategies dealing directly with these stressors, the creation of a workplace that fosters more support and recognition of nurses and promotes professional growth may also help to reduce the impact of work stress for emergency nurses.
Validity of Upper Gastro-Intestinal Endoscopic Screening in HCV Cirrhotic Patients Awaiting Antiviral Therapy

Background and Aim: The Egyptian Ministry of Health initiated a nationwide HCV treatment program with the newly developed oral antiviral therapies and formulated national guidelines for treatment allocation which gave favor for patients with advanced fibrosis and early cirrhosis. One of the recommendations for treatment was upper Gastro-intestinal (GIT) endoscopy. This study aimed at estimating the prevalence of varices among those patients and judging the validity of this national recommendation. Methods: This study was carried out at gastrointestinal endoscopy units, Zagazig University Hospitals through the year 2014. The epidemiologic, clinical features and endoscopic findings of patients undergoing preparation for HCV therapy were analyzed. Endoscopic classifications of esophageal and gastric varices were carried out after the Italian liver cirrhosis project and Sarin’s classification respectively. Results: Totally 1143 patients performed upper GIT endoscopy as preparation for HCV treatment. This comprised 22% of all patients undergoing upper GIT endoscopy over that year. There was a fourfold rise in percentage of patients undergoing endoscopy for sofosbuvir-based therapy in this year (22%) when compared to assessment for Interferon/Ribavirin combination therapy (5%) in the previous year. A total of 361 patients had no esophageal or gastric varices. Small sized (grade I), medium sized (grade II) and large sized (grade III) varices were reported in 301, 188 and 293 patients respectively. Thirty patients (2.6%) had gastric varices. The prevalence of varices was higher in Child B in comparison to Child A (statistically not significant, p = 0.243). Conclusion: Screening endoscopy for early cirrhotic patients awaiting oral anti-HCV therapy is valid.