Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of rhynchophylline and isorhynchophylline on the proliferation of rat vascular smooth muscle cell induced by angiotensin II

Aim To study the effect of rhynchophylline and isorhynchophylline on the proliferation of rat vascular smooth muscle cell(VSMCs)induced by angiotensin Ⅱ(AngⅡ)and discuss it's mechanism.Methods A cell proliferating model of VSMC induced by AngⅡ was established.The proliferative activity of VSMC with rhynchophylline and isorhynchophylline was analyzed by MTT method.The effect of rhynchophylline and isorhynchophylline on cell cycle of VSMC was observed by flow cytometry.The effect of rhynchophylline and isorhynchophylline on protein of AT1R gene,NF-κB gene,Stat3 gene,c-Myc gene and c-Fos gene in VSMCs was observed by flow cytometry.The effect of rhynchophylline and isorhynchophylline on c-Myc mRNA and c-Fos mRNA in VSMCs was observed by RT-PCR.Results The proliferation of VSMC induced by AngⅡ stimulation was inhibited by rhynchophylline and isorhynchophylline,and the cell numbers of G0/G1 phase were increased and those of S phase were decreased markedly.At the same time,high expression of the protein of c-Myc gene,c-Fos gene,AT1R gene,NF-κB gene and stat3 gene stimulated by AngⅡ could be inhibited by rhynchophylline and isorhynchophylline significantly,and high expression of c-Myc gene mRNA and c-Fos gene mRNA stimulated by AngⅡ could also be inhibited by rhynchophylline and isorhynchophylline significantly.Conclusions The proliferation of VSMCs induced by AngⅡ can be inhibited by rhynchophylline and isorhynchophylline.The mechanisms are related to preventing VSMCs from G0/G1 phase entering S phase,and down regulating the expression of protein of AT1R gene,NF-κB gene,Stat3 gene,c-Myc gene and c-Fos gene.In addition,c-Myc gene mRNA and c-Fos gene mRNA expression are down regulated as well.
Loss of Time in the Treatment Adherence Process: A Qualitative Study in a Sample of Iranian People with Diabetes

Diabetes is a significant public health problem and one of the causes of death and disability globally. One of the main problems with diabetes control is the lack of adherence to therapeutic regimens in people with diabetes. This study investigates the experiences and views of the Iranian people with diabetes to identify the challenges of the process of adherence to treatment. A grounded theory research design was used, incorporating in-depth interviews to collect the data. Using purposeful sampling, 28 people with type 2 diabetes (9 men, 19 women) from different places were included in the study. Constant comparative analysis was undertaken to identify key categories. The main challenge in this process is losing the golden time of preventing the complications of the disease that occurs for the following reasons: cultural habits and values, religious beliefs (believing diabetes was God’s will), resistance to change due to age, job conditions, lack of harmony in the family, and non-shared decision-making in the health system. People with diabetes go through trial and error in order to achieve awareness and insight, and consequently, adherence to treatment. Therefore, they need help and support to achieve insight and adherence to treatment faster and without complications. In fact, if the care plan is designed to encourage active patient participation by the treatment team in the shortest possible time, the time to achieve compliance will be shorter and will have the least side effects for these people.
Symptomatic amyloid‐related imaging abnormalities in an APOE ε4/ε4 patient treated with aducanumab

Abstract Introduction Amyloid‐related imaging abnormalities (ARIA) are a common, dose‐dependent effect of amyloid‐targeting antibodies, strongly associated with the apolipoprotein E (APOE) ε4 allele. Methods We describe the clinical course and management of a 66‐year‐old white male (APOE ε4/ε4) enrolled in an observational study that included amyloid and tau positron emission tomography (PET), who received aducanumab through the ENGAGE clinical trial. Results Acute symptoms included headache and encephalopathy, and magnetic resonance imaging revealed ARIA‐E and ARIA‐H. Malignant hypertension and epileptiform activity were treated with nicardipine and levetiracetam. Subsequent clinical/imaging worsening prompted a course of methylprednisolone. Symptoms and ARIA‐E resolved over 6 months, while ARIA‐H persisted. Quantitative analysis of interval amyloid PET showed reduced signal in pre‐existing areas but increased signal posteriorly; while tau PET showed increased signal overall. Discussion In an APOE ε4/ε4 patient, ARIA symptoms were accompanied by malignant hypertension and epileptiform activity, and pulsed steroids reversed edema. Studies from larger cohorts may clarify the optimal treatment and pathophysiology of ARIA.
Abundance of Pest Complex of Okra in Relation to Abiotic and Biotic Factors

Major insect pests of okra viz., leafhopper, (Amrasca biguttula biguttula); whitefly, (Bemisia tabaci); red spider mite, (Tetranychus cinnabarinus) and fruit borer, (Earias vittella) damaged heavily during the kharif seasons. Leafhopper and whitefly occurred from last week of July. The infestation of okra fruit borer was observed during middle of August, whereas the red spider mite appeared during 1st week of September. Peak incidence of leafhopper (217.00 nymphs/15 leaves), whitefly (28.90/15 leaves), red spider mite (231.10 mites/cm2) and fruit borer (56. 6%) during last week of August, 1st week of August, 3rd week of September and 3rd week of September, respectively. The incidence of leafhopper and fruit borer showed significant negative correlation with relative humidity (r =-0.673 &-0.906) and significant positive correlation with sunshine hours. Positive correlation was observed between the minimum (r = +0.994) and mean temperature (r = +0.889) with the density count of whitefly, whereas fruit borer damage was significant negatively correlated with evening relative humidity (r =-0.862). Among biotic factors viz., coccinellids (r = +0.874) and spiders (r = +0.907) there was significant positive correlation with leafhopper population, whereas the fruit infestation was significant negatively correlated with coccinellids (r =-0.911) and spiders (r =-0.923).
The noncoding and coding transcriptional landscape of the peripheral immune response in patients with COVID‐19

Abstract Background COVID‐19 is currently a global pandemic, but the response of human immune system to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection remains unclear. Noncoding RNAs serve as immune regulators and thus may play a critical role in disease progression. Methods We performed multi‐transcriptome sequencing of both noncoding RNAs and mRNAs isolated from the red blood cell depleted whole blood of moderate and severe COVID‐19 patients. The functions of noncoding RNAs were validated by analyses of the expression of downstream mRNAs. We further utilized the single‐cell RNA‐seq data of COVID‐19 patients from Wilk et al. and Chua et al. to characterize noncoding RNA functions in different cell types. Results We defined four types of microRNAs with different expression tendencies that could serve as biomarkers for COVID‐19 progress. We also identified miR‐146a‐5p, miR‐21‐5p, miR‐142‐3p, and miR‐15b‐5p as potential contributors to the disease pathogenesis, possibly serving as biomarkers of severe COVID‐19 and as candidate therapeutic targets. In addition, the transcriptome profiles consistently suggested hyperactivation of the immune response, loss of T‐cell function, and immune dysregulation in severe patients. Conclusions Collectively, these findings provide a comprehensive view of the noncoding and coding transcriptional landscape of peripheral immune cells during COVID‐19, furthering our understanding and offering novel insights into COVID‐19 pathogenesis.
A new species of dromaeosaurids from the Yixian Formation of western Liaoning

A nearly complete specimen of dromaeosaurid has been recently collected from the Yixian Formation near Toutai in Yixian County,western Liaoning.It shows many features of Sinornithosaurus,e.g.the presence of ornament-like pits on the anterolateral surface of the antorbital fossa,unserrated premaxillary teeth,dentary bifurcated posteriorly,manual phalanx II-I-1slightly more than twice the length of phalanx III-2,and partially arctometatarsalian metatarsal III.But this new specimen differs from S.millenii in some bones.So a new species,S.haoiana sp.nov.,is erected based on this specimen.It can be distin guished from S.millenii in that:(1)the main body of the premaxilla is higher,its length being slightly longer than its height ;(2)the anterior margin of the premaxilla is vertical;(3)the maxillary process of the premaxilla is very long;(4)the maxilla is separated from external naris;(5)maxillary fenestra is circular and relatively small;(6)the ascending process of quadratojugal is remarkably longer than the jugal process;(7)the ratio of the dentary length/height is distinctly small;and(8)the pubic peduncle of ilium is longitudinally narrower than acetabulum and so on.There differences indicate that the specimen from Toutai should be a new species of Sinornithosaurus and so is named S.haoiana sp.nov.
Anti-viral effects of active compounds from Antrodia camphorata on wild-type and lamivudine-resistant mutant HBV

Niu-Chang-Chih, which is the fruiting body of Antrodia camphorata, is a folk medicine that has traditionally been used in Taiwan for treating food and drug poisoning, liver disease, and cancer. By using HBV producing cell line as an in vitro model system, we found that Niu-Chang-Chih displays anti-HBV effects on both wild-type and lamivudine-resistant mutants. The active compounds responsible for the anti-viral activities of Niu-Chang-Chih were further investigated and identified by anti-HBV assay directed fractionation. Specifically, out of the ten pure compounds isolated from Niu-Chang-Chih which includes one biphenyl, four ergostanes, and five lanostanes derivatives, 2,2',5,5'-tetramethoxy-3,4,3',4'-bis(methlylene-dioxy)-6,6'-dimethyl-biphenyl was positively identified as the single active compound responsible for the anti-viral effects on both wild-type and lamivudine-resistant mutant HBV.
OROFACIAL CLEFTS AND CARDIOVASCULAR RISK AND DISEASES: THE CAUSAL RELATIONSHIP AND ASSOCIATIONS

There is a complex interplay between orofacial clefts (OFCs) or cleft of the lip and palate and cardiovascular risk factors and cardiac diseases. The presence of maternal cardiovascular risk factors serves as a potent predisposing factor to the development of OFCs during foetal development in addition to the fact that various congenital anomalies are associated with OFCs either in syndromic or non-syndrome relationship. This article narratively explores this complex interplay, which is not uncommon.
Short‐term decline of Castanopsis fargesii adult trees promotes conspecific seedling regeneration: The complete process from seed production to seedling establishment

Abstract Declining forests usually face uncertain regeneration dynamics and recovery trajectories, which are challenging to forest management. In this study, we investigated the decline pattern of Castanopsis fargesii and examined the effects on conspecific seedling regeneration. We found that 61.45% of adult individuals were in decline and the smaller DBH size classes of trees (10–40 cm) had a greater probability of decline. Most of the intermediate decline (94.52%) and nondecline individuals (95.23%) did not worsen, and the crowns of 21.91% of the intermediate decline trees were recovered during 2013–2018. Adult tree decline had a negative effect on seed production (mean mature seed density of nondecline, intermediate decline, and high decline individuals was 167.3, 63.3, and 2.1 seeds/m2, respectively), but no effect on key seed traits. The seed survival rate of declining trees was greater than that of nondeclining trees at both the seed production and seed dispersal stages. The seed to seedling transition rates in canopy gaps, decline habitats, and nondecline habitats were 7.94%, 9.47%, and 109.24%, respectively. The survival rate and height growth of newly germinated seedlings were positively correlated with the light condition, which was notably accelerated in the canopy gaps. Taken together, these results indicate that the reduction in seed production of some adult trees had a weakly negative effect on new seedling recruitment, while the improved environmental condition after the decline significantly enhanced the survival and growth of both advanced and new germinated seedlings. Looking at the overall life history, the short‐term defoliation and mortality of some C. fargesii adult trees can be regarded as a natural forest disturbance that favors conspecific seedling regeneration. High‐intensity management measures would be unnecessary in cases of an emerging intermediate decline in this forest.
Serotype replacement in Streptococcus pneumoniae after conjugate vaccine introduction: impact, doubts and perspective for new vaccines

The licensed seven-valent pneumococcal conjugate vaccine has successfully reduced invasive pneumococcal disease (IPD) in the USA and in all countries in which it was introduced into national immunization programs, but an increase in the incidence of IPD caused by nonvaccine serotypes has been observed in some areas. This review extended investigation into the effects of PCV7 vaccination on the dynamics of IPD and on the epidemiological picture of circulating serotypes, focusing on mechanisms of pneumococcal escape from vaccination, their impact in terms of reduced effectiveness of PCV7 and the perspective for new vaccines.
Immune contexture analysis in immuno‐oncology: applications and challenges of multiplex fluorescent immunohistochemistry

The tumor microenvironment is an integral player in cancer initiation, tumor progression, response and resistance to anti‐cancer therapy. Understanding the complex interactions of tumor immune architecture (referred to as ‘immune contexture’) has therefore become increasingly desirable to guide our approach to patient selection, clinical trial design, combination therapies, and patient management. Quantitative image analysis based on multiplexed fluorescence immunohistochemistry and deep learning technologies are rapidly developing to enable researchers to interrogate complex information from the tumor microenvironment and find predictive insights into treatment response. Herein, we discuss current developments in multiplexed fluorescence immunohistochemistry for immune contexture analysis, and their application in immuno‐oncology, and discuss challenges to effectively use this technology in clinical settings. We also present a multiplexed image analysis workflow to analyse fluorescence multiplexed stained tumor sections using the Vectra Automated Digital Pathology System together with FCS express flow cytometry software. The benefit of this strategy is that the spectral unmixing accurately generates and analyses complex arrays of multiple biomarkers, which can be helpful for diagnosis, risk stratification, and guiding clinical management of oncology patients.
Examination of the Relationship between Certain Gene Variations and Psychological Factors with Physical Activity Level in the Individuals with the Diagnosis of Schizophrenia and Bipolar Disorders

The research aims to examine certain gene variations and to examine relationship between psychological factors and activity levels by the individuals with schizophrenia and bipolar diagnosis. This research has been conducted with the participation of the patients with schizophrenia and bipolar diagnosis in the Mental Facility of Bursa Dört Çelik at the University of Health Science and all the patients take part in physical exercise activities regularly. During the research, IPAQ has been applied to determine participants’ physical activity levels whereas Beck’s Depression Inventory, Beck’s Anxiety Inventory, Staı Form TX-I-II, Beck’s Hopelessness Index has been applied to determine their psychological status after socio-demographic information of participants was obtained by demographic form. In order to examine properties of the participants genetically, oral swabs were obtained by SWAP kite. Genetic variations of cases have been recurred as a result of genotyping analysis of MAO-A, COMT, TH and SLC6A4 genes in GenoFeel panel by using isolated DNAs yielded from swap samples. When the results obtained from statistical analysis are analyzed, it has been seen that genetic properties pair with psychological factor meanwhile the characteristics of diseases bear a resemblance to these measurement. It was determined in this research that, genetic factor were matched with psychological factors, these measurement results resembled with the characteristic of diagnosed ailments as well and schizophrenia and bipolar patients have significance in MAO-A and COMT figures, excessive activation of TH (tyrosine hydroxylase) enzyme may also be included in excessive dopamine production, Homozygote mutation and heterozygote genotypes of gene which is related to SLC6A has rational increase whereas no significance was observed in schizophrenia patient’s group. As a consequence it shouldn’t be neglected that exercising should be a part of schizophrenia and bipolar patients’ life, that exercise, genetic parameters and psychological tests have positive contributions for treatments and that medical treatment has positive contributions with exercise.
Comparative study between conventional fistulectomy and SLOFT in cases of low fistula in ano

Introduction: Fistula in ano is a common anorectal disorder requiring surgical intervention. It is associated with high recurrence rate and post-operative complications. In this study we have analysed and compare results of a newer technique for low anal fistula SLOFT with conventional Fistulectomy. Aims and Objective: To compare Submucosal Ligation of Fistula Tract (SLOFT) with conventional fistulectomy in patients of low anal fistula by analysing various intra-operative factors and post-operative outcome. Materials and Methods: An interventional study carried out at Santosh Medical College and Hospitals, Ghaziabad from January 2017 to October 2018. This study included 60 Patients of low anal fistula which were divided into two groups of 30 patients each. Group I was offered Conventional Fistulectomy & Group II underwent SLOFT. Result and Observations: Fistula in ano is commonly seen in middle aged males. There is no significant difference in duration of surgery between SLOFT and Fistulectomy. However post-operative complications, pain (measured by VAS scoring system), wound healing and early return to work was significantly better in patients who underwent SLOFT. Conclusion: SLOFT has significant advantage over convention fistulectomy in terms of post-operative pain, wound healing, early return to work and cosmetic outcome. Keywords: Fistula in ano, Low anal fistula, SLOFT (Submucosal ligation of fistula tract), LIFT (Ligation of intersphincteric fistula tract), Fistulectomy.
Incidence of lung malignancy in a tertiary care centre – A one year study

Background: Lung cancer is the most common type of cancer in males and the leading cause of death in both sexes. It accounts for 11.3% of all new cancers in India and also associated with a mortality rate of 13.7%. This desolate mortality necessitates for the early diagnosis of lung cancer. The study was conducted to evaluate incidence, gender and age wise distribution of lung cancer and its association with smoking. Methodology: This study was done in the department of pulmonary medicine in Guntur Medical College from September 2016 to august 2017. The study includes 52 patients having clinical and radiological suspicion of lung cancer. All the cases were subjected to USG guided transthoracic needle aspiration was sent for cytology. Inconclusive and malignant cases were further subjected to histopathological examination for confirmation. Results: Out of the 52 patients suspicious of malignancy, 32 patients were finally diagnosed as malignant, 5 patients had benign/inflammatory lesions, 6 cases had no evidence of malignancy/non-specific inflammation, 8 had no opinion possible. Most common type is adenocarcinoma followed by squamous cell carcinoma with an incidence rate of 71.8% & 15.7% respectively. The highest incidence of lung malignancies is seen in 5 decade in both sexes. Conclusions: There is increase in the incidence of lung malignancies in females, throwing light on the causes other than smoking like biomass fuel exposure and second-hand smoking. Adenocarcinoma is the most common type. Further, USG guided transthoracic needle aspiration has a good sensitivity in detecting lung malignancies.
Sodicity Tolerance of Grapes with Reference To the Uptake of Nutrients

Relative performance and uptake of nutrients in Perlett, Beauty seedless, Delite and Thompson seedless at 4 levels of exchangeable sodium percentage (ESP) i.e. 13, 22, 39 and 67 have been evaluated in a pot culture experiment in a sandy loam soil (Naturstalf). K, Ca and Mg contents decreased whereas P, Zn, Fe and Cu increased with sodicity. Sodic conditions of the soil plant continium affected the translocation of Mn, K and Na among root, shoot and leaves, differentially. In terms of root weight Thompson seedless was comparatively more tolerant because it could maintain high K/Na ratio in leaves, canes and especially roots. However from aerial growth, Delight could tolerate higher sodicity. Cultivar differences in the uptake of Na, K, P (except leaves), Ca (except leaves), Mg, Zn, Cu (roots only), Fe and Mn (except canes), were significant. Sodicity × cultivar inter actions were also significant for P, Zn, Cu, Fe and Mn contents in various parts of the grape vine. It therefore appears that appropriate root stocks and scionic combinations can be used for soaling up sodicity tolerance.
Association of genetic and climatic variability in giant sequoia, Sequoiadendron giganteum, reveals signatures of local adaptation along moisture‐related gradients

Abstract Uncovering the genetic basis of local adaptation is a major goal of evolutionary biology and conservation science alike. In an era of climate change, an understanding of how environmental factors shape adaptive diversity is crucial to predicting species response and directing management. Here, we investigate patterns of genomic variation in giant sequoia, an iconic and ecologically important tree species, using 1,364 bi‐allelic single nucleotide polymorphisms (SNPs). We use an F ST outlier test and two genotype–environment association methods, latent factor mixed models (LFMMs) and redundancy analysis (RDA), to detect complex signatures of local adaptation. Results indicate 79 genomic regions of potential adaptive importance, with limited overlap between the detection methods. Of the 58 loci detected by LFMM, 51 showed strong correlations to a precipitation‐driven composite variable and seven to a temperature‐related variable. RDA revealed 24 outlier loci with association to climate variables, all of which showed strongest relationship to summer precipitation. Nine candidate loci were indicated by two methods. After correcting for geographic distance, RDA models using climate predictors accounted for 49% of the explained variance and showed significant correlations between SNPs and climatic factors. Here, we present evidence of local adaptation in giant sequoia along gradients of precipitation and provide a first step toward identifying genomic regions of adaptive significance. The results of this study will provide information to guide management strategies that seek to maximize adaptive potential in the face of climate change.