Miracle-dz/UniFAR-FTUs huggingface dataset:
Hierarchical Micro-/Nano-Structures on Polycarbonate via UV Pulsed Laser Processing

Hierarchical micro/-nanostructures were produced on polycarbonate polymer surfaces by employing a two-step UV-laser processing strategy based on the combination of Direct Laser Interference Patterning (DLIP) of gratings and pillars on the microscale (3 ns, 266 nm, 2 kHz) and subsequently superimposing Laser-induced Periodic Surface Structures (LIPSS; 7–10 ps, 350 nm, 100 kHz) which adds nanoscale surface features. Particular emphasis was laid on the influence of the direction of the laser beam polarization on the morphology of resulting hierarchical surfaces. Scanning electron and atomic force microscopy methods were used for the characterization of the hybrid surface structures. Finite-difference time-domain (FDTD) calculations of the laser intensity distribution on the DLIP structures allowed to address the specific polarization dependence of the LIPSS formation observed in the second processing step. Complementary chemical analyzes by micro-Raman spectroscopy and attenuated total reflection Fourier-transform infrared spectroscopy provided in-depth information on the chemical and structural material modifications and material degradation imposed by the laser processing. It was found that when the linear laser polarization was set perpendicular to the DLIP ridges, LIPSS could be formed on top of various DLIP structures. FDTD calculations showed enhanced optical intensity at the topographic maxima, which can explain the dependency of the morphology of LIPSS on the polarization with respect to the orientation of the DLIP structures. It was also found that the degradation of the polymer was enhanced for increasing accumulated fluence levels.
Dogs and Their Owners Have Frequent and Intensive Contact

Contact and interactions between owners and their pets may have beneficial physical and social effects on people, but may also facilitate the transmission of zoonotic agents and resistant bacteria. To estimate the risk of these contacts, more information regarding the frequency and intensity of this physical contact is required. Therefore, an online survey was conducted among pet owners resulting in 701 completed questionnaires. Questions regarding the interactions between dogs and owners were linked with a score from 1 (limited interactions) to 3 (highly intense interactions). After scoring these self-reported interactions, a contact intensity score was calculated for each respondent by summing up the different allocated scores from all questions. This contact intensity score was used to identify predictors of more intense contact based on a multivariable linear regression model. Interactions between dogs and their owners were widespread (e.g., 85.3% of the dogs licked their owner’s hand) and intense (e.g., 49.3% of owners reported being licked in the face). The gender, age, and place of residence (city, village, or countryside) of the respondent, together with the size and age of the dog, were significantly associated with the contact intensity score in the multivariable model. On average, female respondents younger than 65 years who lived in the city and had a small young dog had the most intense contact with it. Further research is necessary to evaluate the risk of these interactions in light of zoonotic and antimicrobial resistance transfer.
Parental Socialization and Its Impact across the Lifespan

Classical studies have found that parental warmth combined with parental strictness is the best parental strategy to promote children’s psychosocial development. Nevertheless, a growing set of emergent studies has questioned the benefits of parental strictness. The present study examined parental socialization and its short- and long-term impact on the psychosocial development of adolescents and adult children. The sample consisted of 2150 Spanish participants, 623 adolescents (12–18 years), 619 young adults (19–35 years), 502 middle-aged adults (35–59 years), and 406 older adults (60 years or older). Families were classified into one of four typologies (indulgent, authoritative, authoritarian, and neglectful). Psychosocial development was examined with five indicators (physical and family self-concept, nervousness, empathy, and internalization of social values of benevolence). The results show a common short- and long-term pattern between parenting styles and psychosocial development: the indulgent style equaled or even surpassed the authoritative style, whereas the neglectful and authoritarian styles were associated with low scores. The present findings were discussed by considering the importance of the cultural context in family socialization. Additionally, the long-term impact of parental socialization seems to be crucial, even in adulthood.
Targeting Pharmacokinetic Drug Resistance in Acute Myeloid Leukemia Cells with CDK4/6 Inhibitors

Pharmacotherapy of acute myeloid leukemia (AML) remains challenging, and the disease has one of the lowest curability rates among hematological malignancies. The therapy outcomes are often compromised by the existence of a resistant AML phenotype associated with overexpression of ABCB1 and ABCG2 transporters. Because AML induction therapy frequently consists of anthracycline-like drugs, their efficiency may also be diminished by drug biotransformation via carbonyl reducing enzymes (CRE). In this study, we investigated the modulatory potential of the CDK4/6 inhibitors abemaciclib, palbociclib, and ribociclib on AML resistance using peripheral blood mononuclear cells (PBMC) isolated from patients with de novo diagnosed AML. We first confirmed inhibitory effect of the tested drugs on ABCB1 and ABCG2 in ABC transporter-expressing resistant HL-60 cells while also showing the ability to sensitize the cells to cytotoxic drugs even as no effect on AML-relevant CRE isoforms was observed. All tested CDK4/6 inhibitors elevated mitoxantrone accumulations in CD34+ PBMC and enhanced accumulation of mitoxantrone was found with abemaciclib and ribociclib in PBMC of FLT3-ITD- patients. Importantly, the accumulation rate in the presence of CDK4/6 inhibitors positively correlated with ABCB1 expression in CD34+ patients and led to enhanced apoptosis of PBMC in contrast to CD34− samples. In summary, combination therapy involving CDK4/6 inhibitors could favorably target multidrug resistance, especially when personalized based on CD34− and ABCB1-related markers.
Study of galacto‐oligosaccharide formation from lactose using Pectinex Ultra SP‐L

BACKGROUND:Galacto-oligosaccharides (GOS) are synthesised from lactose by transglycosylation using β-galactosidase (EC 3.2.1.23) and are recognised as prebiotics. The commercial enzyme preparation Pectinex Ultra SP-L produced by Aspergillus aculeatus possesses β-galactosidase activity; however, because its use has been directed towards the formation of 6′-β-galactosyl-lactose, no data have been reported on the formation of other GOS. Since the composition of the oligosaccharide mixture obtained during lactose hydrolysis may affect the prebiotic properties, in this study the influence of various parameters (pH, temperature, time and enzyme and lactose concentrations) on the formation of GOS using Pectinex Ultra SP-L was investigated. RESULTS:High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis allowed the detection of disaccharides other than lactose, trisaccharides and minor amounts of higher-molecular-weight GOS. The main GOS formed were a trisaccharide identified as β-D-Galp-(1 → 6)-Lac (6′-β-galactosyl-lactose) and a disaccharide identified as β-D-Galp-(1 → 6)-D-Gal (galactobiose). Other GOS detected were tentatively identified as β-D-Galp-(1 → 6)-D-Glc (allolactose), β-D-Galp-(1 → 3)-D-Glc and β-D-Galp-(1 → 3)-Lac. Trisaccharide formation was favoured by a pH increase from 4.5 to 6.5, whereas the disaccharide content increased as the pH decreased, reaching a level of 11% at pH 4.5. 6′-β-Galactosyl-lactose production increased gradually with increasing temperature, attaining a maximum value of 17% at 60 °C after 7 h, whereas disaccharide formation was optimal at 50 °C, reaching a level of 10% after 24 h. CONCLUSION:The results indicate that Pectinex Ultra SP-L can be used to obtain GOS mixtures of different composition depending on the operating conditions. It has been shown for the first time that Pectinex Ultra SP-L can be used for the selective formation of disaccharides. Copyright © 2008 Society of Chemical Industry
A Multistage Formulation Based on Full-Length CSP and AMA-1 Ectodomain of Plasmodium vivax Induces High Antibody Titers and T-cells and Partially Protects Mice Challenged with a Transgenic Plasmodium berghei Parasite

Infections with Plasmodium vivax are predominant in the Americas, representing 75% of malaria cases. Previously perceived as benign, malaria vivax is, in fact, a highly debilitating and economically important disease. Considering the high complexity of the malaria parasite life cycle, it has been hypothesized that an effective vaccine formulation against Plasmodium should contain multiple antigens expressed in different parasite stages. Based on that, we analyzed a recombinant P. vivax vaccine formulation mixing the apical membrane antigen 1 ectodomain (PvAMA-1) and a full-length circumsporozoite protein (PvCSP-AllFL) previously studied by our group, which elicits a potent antibody response in mice. Genetically distinct strains of mice (C57BL/6 and BALB/c) were immunized with the proteins, alone or in combination, in the presence of poly(I:C) adjuvant, a TLR3 agonist. In C57BL/6, high-antibody titers were induced against PvAMA-1 and the three PvCSP variants (VK210, VK247, and P. vivax-like). Meanwhile, mixing PvAMA-1 with PvCSP-AllFL had no impact on total IgG antibody titers, which were long-lasting. Moreover, antibodies from immunized mice recognized VK210 sporozoites and blood-stage parasites by immunofluorescence assay. However, in the BALB/c model, the antibody response against PvCSP-AllFL was relatively low. PvAMA-1-specific CD3+CD4+ and CD3+CD8+ T-cell responses were observed in C57BL/6 mice, and the cellular response was impaired by PvCSP-AllFL combination. More relevant, the multistage vaccine formulation provided partial protection in mice challenged with a transgenic Plasmodium berghei sporozoite expressing the homologous PvCSP protein.
Morphometric criteria for estimating sexual maturity in two snappers, Etelis carbunculus and Pristipomoides sieboldii

A gross measure of repro- ductive condition (ovary weight ad- justed for body size and oocyte volume) is developed and evaluated as an alter- native to commonly used gonad indices, for classifying the maturity status of in- dividual ehu (Etelis carbunculus) and kalekale (Pristipomoides sieboldii), two species of eteline snappers (Lutjanidae) that contribute to the deep-slope hand- line fishery in Hawaii. Discriminant analysis and logistic regression, based on body length, ovary weight, and oo- cyte volume, were used to classify fish as either immature or mature. Dis- criminant analysis correctly classified the maturity of about 98% of both ehu and kalekale, with histological criteria as the standard for comparison. Logis- tic regression correctly classified ma- turity of 97% of the ehu and 100% of the kalekale. Misclassification errors increased by 3.75-5% (discriminant analysis) or 0-5% (logistic regression) if oocyte volume was excluded and only body length and ovary weight were used as predictors of maturity. For kalekale, estimates of lengths at
Importance of the Role of ω-3 and ω-6 Polyunsaturated Fatty Acids in the Progression of Brain Cancer

Brain cancer is one of the most malignant types of cancer in both children and adults. Brain cancer patients tend to have a poor prognosis and a high rate of mortality. Additionally, 20–40% of all other types of cancer can develop brain metastasis. Numerous pieces of evidence suggest that omega-3-polyunsaturated fatty acids (ω-PUFAs) could potentially be used in the prevention and therapy of several types of cancer. PUFAs and oxylipins are fundamental in preserving physiological events in the nervous system; it is, therefore, necessary to maintain a certain ratio of ω-3 to ω-6 for normal nervous system function. Alterations in PUFAs signaling are involved in the development of various pathologies of the nervous system, including cancer. It is well established that an omega-6-polyunsaturated fatty acid (ω-6 PUFA)-rich diet has a pro-tumoral effect, whereas the consumption of an ω-3 rich diet has an anti-tumoral effect. This review aims to offer a better understanding of brain cancer and PUFAs and to discuss the role and impact of PUFAs on the development of different types of brain cancer. Considering the difficulty of antitumor drugs in crossing the blood–brain barrier, the therapeutic role of ω-3/ω-6 PUFAs against brain cancer would be a good alternative to consider. We highlight our current understanding of the role of PUFAs and its metabolites (oxylipins) in different brain tumors, proliferation, apoptosis, invasion, angiogenesis, and immunosuppression by focusing on recent research in vitro and in vivo.
Seasonal dynamics of subtidal macrophyte assemblages in Sobol Bay (Peter the Great Bay, Sea of Japan) in relation to depth

This paper describes the species composition, vertical distribution, and the seasonal changes in the biomass of subtidal macrophytes in Sobol Bay (Peter the Great Bay, Sea of Japan). The samples were collected in the depth-range from 0.5 to 4 m. A total of 65 macrophyte species were identified, among which were 10 species of Chlorophyta, 17 of Phaeophyceae, 37 of Rhodophyta and 1 of Magnoliophyta. Multivariate analyses were performed to detect spatial and temporal variations. Maximum species richness was registered in June, with a particularly dramatic biomass increase of brown algae. Both the number of species and the biomass of macrophytes decreased with increasing depth. Depth clearly affected the patterns of seasonal fluctuations of the species composition and biomass. In the study area, the species composition of the shallow-water algal assemblages was more stable throughout the year compared to that of the algal assemblages found in deeper waters. In total, four macrophyte assemblages were identified in the bay. A Phyllospadix iwatensis and Coccophora langsdorfii dominated assemblage occupied muddy-gravel bottoms in depths from 0.5 to 2 m throughout the year. An assemblage, co-dominated by annual brown algae Desmarestia viridis and Costaria costata, occurred at depths more than 3 m at late spring. Two other assemblages occurred on the rock and boulder bottom at 0.5–2 m depths, but they were temporally separated. An assemblage co-dominated by annual laminarian algae (Undaria pinnatifida and Costaria costata) developed at late spring and was succeeded in the autumn and winter by a Tichocarpus crinitus, Ulva lactuca, Sargassum pallidum and Codium fragile co-dominated assemblage. A comparison between the data obtained in the present study and the results of a previous study conducted in 1927 shows that the macrophytes assemblages in Sobol Bay underwent little change.
The Regulation of the Staphylococcus aureus putP Gene

Staphylococcus aureus is a significant cause of human infections. The species needs to import the amino acid proline from the outside to grow and survive. To accomplish this task, S. aureus has two proline transport systems, one of which is encoded by the putP g e n e . I n t h i s s t u d y , t h e regulation of the S. aureus putP gene was examined. The promoter for the S. aureus putP gene was PCR amplified from genomic DNA of strain RN6390 and the product ligated to a promoterless lacZ gene. Subsequently, this putP-lacZ fusion was then inserted into the plasmid pMOD-1, forming pUB1-1. This pUB1-1 plasmid was electroporated into S. aureus strain RN4220 cells and one clone was tested under different growth conditions that had variations in proline concentrations or osmolarities. An increase in osmolarity led to an increase in expression of the putP gene in S. aureus. Moreover, limiting proline in the medium also caused increased putP expression. Our results suggest that osmolarity and low proline concentrations, conditions one might encounter in the human body, appear to activate the putP gene in S. aureus. This supports the role of putP expression in the in vivo survival of S. aureus.
Broiler Chicks’ Motivation for Different Wood Beddings and Amounts of Soiling

Simple Summary Many animals move excreta—or feces—away from resting areas to avoid attracting predators and spreading disease. However, today’s farms raise broiler (meat) chickens in large barns with stocking densities that prevent the birds from segregating their excreta. Moreover, whether or not chickens would prefer to avoid their excreta is unknown. Understanding what litter conditions chickens prefer can help inform farming practices. Therefore, this experiment aimed to assess chicks’ motivation to access unsoiled bedding or soiled litter. We used six pens of six to seven broiler chicks—each pen divided into two compartments by a barrier containing two one-way push-doors. The ‘home’ compartment contained soiled wood shavings, while the ‘treatment’ (T) compartment contained either aspen wood shavings, pine and spruce wood shavings, soiled pine and spruce wood shavings, ammonia reductant treated soiled pine and spruce wood shavings, or a feed treatment as a gold standard. To determine the chicks’ motivation to access the resources, the door leading into T weighed 0% (lifted), 10%, 20%, or 30% of the chicks’ body weight. The combination of time spent in T, number of visits to T, and average maximum weight pushed to access T were used to measure motivation. Chicks showed equal motivation for all substrates and preferred feed over all substrates. However, future experiments must explore chicks’ preference and motivation over the long-term in commercial conditions. Abstract In the wild, excreta soiled surroundings can attract predators and spread disease. Yet, farmers rear broiler chicks in large barns with stocking densities that prevent excreta segregation. To measure chicks’ motivation to access unsoiled bedding or soiled litter (collectively, substrates) we used 40 16-day-old broiler chicks who were divided into six two-compartment pens. The ‘home’ compartment (H) contained soiled wood shavings, while the ‘treatment’ compartment (T) contained either aspen wood shavings, pine and spruce wood shavings, soiled pine and spruce wood shavings, ammonia reductant treated soiled pine and spruce wood shavings, or a feed treatment as a gold standard. The barrier separating the compartments had two one-way push-doors that chicks pushed to access a resource. The chicks’ motivation was measured by the average maximum weight pushed to access each resource. The door leading to T weighed 0% (raised), 10%, 20%, or 30% of the chicks’ body weight, and chicks could return to H via a raised (for 0%) or unweighted door. Our findings indicate that chicks worked hardest for feed, but paid a lower, equal price to access all substrates. With increasing door weight, chicks visited less and spent less time with the substrates. Therefore, as chicks themselves do not avoid litter that could have potential negative effects on their well-being, it is important that farmers diligently monitor litter conditions as their primary care-takers.
Sex Differences in Urate Handling

Hyperuricemia, or elevated serum urate, causes urate kidney stones and gout and also increases the incidence of many other conditions including renal disease, cardiovascular disease, and metabolic syndrome. As we gain mechanistic insight into how urate contributes to human disease, a clear sex difference has emerged in the physiological regulation of urate homeostasis. This review summarizes our current understanding of urate as a disease risk factor and how being of the female sex appears protective. Further, we review the mechanisms of renal handling of urate and the significant contributions from powerful genome-wide association studies of serum urate. We also explore the role of sex in the regulation of specific renal urate transporters and the power of new animal models of hyperuricemia to inform on the role of sex and hyperuricemia in disease pathogenesis. Finally, we advocate the use of sex differences in urate handling as a potent tool in gaining a further understanding of physiological regulation of urate homeostasis and for presenting new avenues for treating the constellation of urate related pathologies.
Hydrogen Sulfide Treatment Improves Post-Infarct Remodeling and Long-Term Cardiac Function in CSE Knockout and Wild-Type Mice

Hydrogen sulfide (H2S) is recognized as an endogenous gaseous signaling molecule generated by cystathionine γ-lyase (CSE) in cardiovascular tissues. H2S up-regulation has been shown to reduce ischemic injury, and H2S donors are cardioprotective in rodent models when administered concurrent with myocardial ischemia. We evaluated the potential utility of H2S therapy in ameliorating cardiac remodeling with administration delayed until 2 h post-infarction in mice with or without cystathionine γ-lyase gene deletion (CSE−/−). The slow-release H2S donor, GYY4137, was administered from 2 h after surgery and daily for 28 days following myocardial infarction (MI) induced by coronary artery ligation, comparing responses in CSE−/− with wild-type (WT) mice (n = 5–10/group/genotype). Measures of cardiac function and expression of key genes associated with cardiac hypertrophy, fibrosis, and apoptosis were documented in atria, ventricle, and kidney tissues. Post-MI GYY4137 administration reduced infarct area and restored cardiac function, accompanied by reduction of the elevated ventricular expression of genes mediating cardiac remodeling to near-normal levels. Few differences between WT and CSE−/− mice were observed, except CSE−/− mice had higher blood pressures, and higher atrial Mir21a expression across all treatment groups. These findings suggest endogenous CSE gene deletion does not substantially exacerbate the long-term response to MI. Moreover, the H2S donor GYY4137 administered after onset of MI preserves cardiac function and protects against adverse cardiac remodeling in both WT and CSE-deficient mice.
Postural Stability in Athletes: The Role of Age, Sex, Performance Level, and Athlete Shoe Features

The effects of different factors—such as age, sex, performance level, and athletic shoe features—on postural balance in athletes remain unclear. The main objective of our study is to identify the features of postural stability in athletes of different age, sex, performance level, and using different types of athletic shoes. This study assessed postural stability in athletes (n = 936, 6–47 years) in a normal bipedal stance with eyes open (EO) and eyes closed (EC). Postural stability was evaluated based on the center of pressure (COP), sway area (AS), and velocity (VCP) while standing on a stabiloplatform. Children (6–12 years) and teen athletes (13–17 years) showed reduced AS-EO (p < 0.01) and VCP-EO (p < 0.01) compared to control (n = 225, 7–30 years). In male and female athletes aged 18+, only VCP-EC was lower versus control. In females (13–17 and 18+), VCP-EO and EC were lower than in males (p < 0.05). Only in the Shooting group, the athletes’ performance levels had an effect on VCP-EO (p = 0.020). Long use of rigid athletic shoes with stiff ankle support was associated with reduced posture stability. Postural stability in athletes was mostly influenced by the athlete‘s age, and, to a lesser extent, by their sex, performance level, and athlete shoe features.