Miracle-dz/UniFAR-FTUs huggingface dataset:
PAZ domain is critical for spermatogenesis in chicken

Abstract Piwi-like protein 1 (PIWIL1) plays a crucial role in stem cell proliferation, embryogenesis, growth, and development. We aimed to unravel the function of PIWIL1 and its Piwi/Argonaute/Zwille (PAZ) domain in chicken embryogenesis. The expression of PIWI1 at different stages of spermatogenesis was analyzed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and the PAZ domain was mutated based on its 3D structure model using the clustered regularly interspaced short palindromic repeats Cas9 (CRISPR/Cas9) technology. The results indicated that PIWIL1 mRNA was specifically expressed in spermatogonium cells undergoing meiosis. After targeting the PAZ domain (300–370 amino acid residues), we obtained two mutant DF-1 cell clones with 23-bp and 8-bp deletions. Injection of the pCMV-Cas9-puro-sgRNA-2 construct into 2.5-day embryos resulted in generation of 19 different PAZ mutants (13 males and 6 females), which showed delayed hatching, reduced quality of semen, and decreased expression of PIWIL1 and SOX2 at embryonic days 5 and 18. However, we could not obtain PAZ double knockout (KO) chickens by crossing of the F0 generation, suggesting that PAZ double KO may halt embryonic development. Our results indicate that PIWIL1 plays an important role in meiosis and that PAZ mutations can lead to decreased sperm quality, whereas its double KO may arrest embryogenesis in chicken.
The National Evaluation of Malawi’s PMTCT Program (NEMAPP) study: 24‐month HIV‐exposed infant outcomes from a prospective cohort study

Data on long‐term HIV‐free survival in breastfeeding, HIV‐exposed infants (HEIs) are limited. The National Evaluation of Malawi’s Prevention of Mother‐to‐Child Transmission (PMTCT) Program (NEMAPP), conducted between 2014 and 2018, evaluated mother‐to‐child transmission (MTCT) and infant outcomes up to 24 months postpartum.
The effects of 3D topography on controlled-source audio-frequency magnetotelluric responses

We have investigated the 3D topographic effects on controlledsource audio-frequency magnetotelluric data. Two 3D topographic models are considered: a trapezoidal-hill model and a trapezoidalvalley model. Different responses are generated, including the amplitude of the electric field, the amplitude of the magnetic field, the apparent resistivity, and phase data. The responses distorted by the 3D topography are simulated for the source located next to and on the hill/valley. Our study indicates that all electric field, magnetic field, apparent resistivity, and phase data are influenced by 3D topography, but to different extents. These topographic effects depend on the transmission-receiver-topography geometry, the transmission frequency, earth resistivity, and the roughness of the surface. The effects in the near-field generated by topography in the survey area are quite different from those in the far-field because of the existence of the source. Compared with those in the far-field zone, the magnetic field and phase data in the nearfield zone are less distorted, but more distortions can be found on the electric field and apparent resistivity data over the hill and valley models. Our results also indicate that not only can the 3D topography in the receiver area lead to strong distortions, but also that at the source position can lead to strong distortions. We concluded our study by quantifying the roughness with which the topographic distortion can be ignored, setting the accepted data distortion to a maximum of 10%.
Evaluating the agility of adaptive command and control networks from a cyber complex adaptive systems perspective

Command and control (C2) networks are critical components of modern military systems, enabling information sharing and communications between systems. These systems operate in complex environments characterized by uncertain and evolving threats, creating a need for agile C2 networks. This paper presents a Cyber Complex Adaptive Systems approach for assessing the agility of adaptive C2 networks. Agent-based modeling is used to simulate the performance of a C2 network connecting unmanned aerial vehicles for a collaborative surveillance mission. Due to the importance of information sharing in C2, information entropy-based awareness is used to quantitatively evaluate C2 performance. Complex network methods are used to define initial network topologies and threats. Network adaptation through random rewiring is shown to recover lost C2 capabilities following network attacks, and in some cases improve performance relative to initial topologies. Inverse average path length and largest connected component fraction are shown to be important factors for maintaining C2 awareness, with inverse average path length being the better indicator of awareness.
Revisiting the effectiveness of repetitive transcranial magnetic stimulation treatment in depression, again

Following on from the publication of the Royal Australian and New Zealand Journal of Psychiatry Mood Disorder Clinical Practice Guidelines (2020) and criticisms of how these aberrantly addressed repetitive transcranial magnetic stimulation treatment of depression, questions have continued to be raised in the journal about this treatment by a small group of authors, whose views we contend do not reflect the broad acceptance of this treatment nationally and internationally. In fact, the evidence supporting the use of repetitive transcranial magnetic stimulation treatment in depression is unambiguous and substantial, consisting of an extensive series of clinical trials supported by multiple meta-analyses, network meta-analysis and umbrella reviews. Importantly, the use of repetitive transcranial magnetic stimulation treatment in depression has also been subject to a series of health economic analyses. These indicate that repetitive transcranial magnetic stimulation is a cost-effective therapy and have been used in some jurisdictions, including Australia, in support of public funding. An argument has been made that offering repetitive transcranial magnetic stimulation treatment may delay potentially effective pharmacotherapy. In fact, there is considerably greater danger of the opposite happening. Repetitive transcranial magnetic stimulation is as, if not more effective, than antidepressant medication after two unsuccessful medication trials and should be a consideration for all patients under these circumstances where available. There is no meaningful ongoing debate about the use of repetitive transcranial magnetic stimulation treatment in depression – it is a safe, effective and cost-effective treatment.
Mass spectrometry‐based cortisol profiling during adrenal venous sampling reveals misdiagnosis for subtyping primary aldosteronism

Primary aldosteronism (PA) is a common form of secondary hypertension. Adrenal venous sampling (AVS) is the gold standard for subtyping PA. This study aimed to determine whether there is a difference between immunoassays and liquid chromatography‐mass spectrometry (LC‐MS/MS) methods for measuring cortisol levels that affect the judgement of AVS.
Me first? Positioning self in the attentional hierarchy.

The attention system that allocates resources across competing aspects of the environment is influenced by biases toward particular types of stimuli, such as cues of threat (e.g., angry-face image), self-reference (e.g., own-face image), and current goals (e.g., food image when hungry). Here, we used dot probe tasks to investigate which of these stimulus types are prioritized in the attentional hierarchy, measuring response latency to dot probes presented in the same location as different face types. In Experiment 1, participants (N = 42) were presented with self, angry, and neutral face images in the dot probe task, which revealed a clear attentional bias for self-images over both angry and neutral images. In Experiment 2, each participant (N = 69) was assigned a self, angry, or neutral goal image for a secondary monitoring task designed to induce a temporary goal, and this image was included in the stimuli presented in the dot probe task. Again, self-cues were found to produce a strong attentional bias, but images associated with temporary goals were found to be the most effective source of attentional bias. Results are discussed in relation to the relative importance of self, threat, and temporary goal cues in the attentional hierarchy. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
PHARMACOGNOSTIC STUDIES OF THE COSTUS PICTUS D. DON.

The leaf of Costus pictus D. Don is considered as an antidiabetic in folklore medicine and is known to reduce the blood sugar similar to that of insulin. In present investigation, the detailed pharmacognostic study of C. pictus leaf is carried out to lay down the standards which could be useful in future experimental studies. The study includes macroscopy, microscopy , preliminary phytochemical screening, physicochemical evaluation and fluorescence analysis.
Well-being trajectories of middle-aged and older adults and the corona pandemic: No "COVID-19 effect" on life satisfaction, but increase in depressive symptoms.

The coronavirus disease (COVID-19) pandemic has led to profound changes in individuals' lives and might have meaningful implications for well-being. We investigated if and how two major indicators of well-being (life satisfaction and depressive symptoms) changed in Germany from a prepandemic measurement occasion (2017) to June/July 2020, the time of the fading first wave of COVID-19. This change was compared with prepandemic change between 2014 and 2017. We also analyzed whether change in well-being varied according to age, self-rated health, corona-specific attitudes, and subjective standard of living. Ten thousand seven-hundred and ninety three individuals had taken part in at least one of the measurement occasions between 2014 and 2020 (mean age in 2014: 64.30 years; SD = 11.58 years). Based on latent change score models controlling for baseline well-being and sociodemographic indicators, we found, across both time intervals (2014-2017, 2017-2020) and independent of individuals' age, a small mean-level increase in life satisfaction. In contrast, depressive symptoms increased from 2017 to 2020, particularly among older participants, whereas they remained, on average, stable between 2014 and 2017. Individuals with a poorer self-rated health, who felt more threatened by the pandemic and whose subjective standard of living was lower revealed a decrease in life satisfaction and a steeper increase in depressive symptoms between 2017 and 2020. Our findings thus suggest that whereas life satisfaction remained quite stable, the pandemic was, already 3-4 months after its onset in Germany, accompanied by an increase in depressive symptoms, particularly among adults who were older, felt less healthy and were more concerned about COVID-19. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
Assessment of the Urinary Microbiota of MSM Using Urine Culturomics Reveals a Diverse Microbial Environment.

BACKGROUND The urinary tract is not sterile and is populated by microbial communities that influence urinary health. Men who have sex with men (MSM) are understudied yet have increased risk factors for genitourinary infections. Our objective was to interrogate the composition of MSM urinary microbiota. METHODS Midstream urine specimens (n = 129) were collected from MSM (n = 63) and men seen for routine care (clinical cohort, n = 30). Demographics and sexual/medical history were documented. Specimens underwent culture using standard-of-care and enhanced methods designed to isolate fastidious and anaerobic microorganisms. Isolates were identified by MALDI-TOF mass spectrometry or 16S rRNA gene sequencing. RESULTS The MSM cohort was younger (mean (SD), 35.4 (11.26) years) compared to the clinical cohort (62.7 (15.95) years). Organism recovery was significantly increased using enhanced vs standard culture for the MSM (mean of 9.1 vs 0.6 species/sample [P < 0.001]) and clinical (7.8 vs 0.9 species/sample [P < 0.001]) cohorts. The microbial composition of MSM urine specimens was dominated by Gram-positive and anaerobic microbes and clustered distinctly from that of clinical urine specimens. Composition of microbial species recovered within the same subject was dynamic between urine specimens but more similar relative to inter-individual comparisons. Principal coordinate analysis showed no correlation between urinary microbiota composition and age, recent antibiotic use, sexually transmitted infection/HIV status, or sexual practices. CONCLUSIONS Enhanced culture recovered a large diversity of microbial species from MSM urine specimens, especially taxa typically associated with mucosal surfaces. These findings may increase understanding of urologic disease in MSM and improve diagnostic methods for detection of genitourinary infections.
Title Towards Dataset Dynamics : Change Frequency of LinkedOpen Data Sources

Datasets in the LOD cloud are far from being static in their nature and how they are exposed. As resources are added and new links are set, applications consuming the data should be able to deal with these changes. In this paper we investigate how LOD datasets change and what sensible measures there are to accommodate dataset dynamics. We compare our findings with traditional, document-centric studies concerning the “freshness” of the document collections and propose metrics for LOD datasets.
Attentional control and metacognitive monitoring of the effects of different types of task-irrelevant sound on serial recall.

The presence of task-irrelevant sound disrupts short-term memory for serial information. Recent studies found that enhanced perceptual task-encoding load (static visual noise added to target items) reduces the disruptive effect of an auditory deviant but does not affect the task-specific interference by changing-state sound, indicating that the deviation effect may be more susceptible to attentional control. This study aimed to further specify the role of attentional control in shielding against different types of auditory distraction, examining speech and nonspeech distractors presented in laboratory and Web based experiments. To further elucidate the role of controlled processes, we tested whether the detrimental effects of distractor sounds-and their modulation by attentional control-reach participants' awareness. We found that changing-state sound and auditory deviants in steady-state sound equally affected both objective recall performance and metacognitive confidence judgments but did not affect the accuracy of confidence judgments. Most importantly, across four experiments, an increase of task load (visual degradation of the to-be-remembered items) did not reduce either type of auditory distraction. A close replication of the original modulation of the deviation effect by perceptual task load (in an online environment) even revealed a stronger deviation effect at high task load, suggesting that the manipulation may have influenced cognitive load and the ability to control distractor interference in memory. In line with a unitary account of auditory distraction, the results suggest that although both types of distraction reach metacognitive awareness, they may be equally unrelated to perceptual load and the availability of attentional resources. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
Predictive Value of C‐Reactive Protein and Albumin for Temporal Within‐Individual Pharmacokinetic Variability of Voriconazole in Pediatric Patients Undergoing Hematopoietic Cell Transplantation

Voriconazole is a widely used antifungal agent in immunocompromised patients, but its utility is limited by its variable exposure and narrow therapeutic index. Population pharmacokinetic (PK) models have been used to characterize voriconazole PK and derive individualized dosing regimens. However, determinants of temporal within‐patient variability of voriconazole PK were not well established. We aimed to characterize temporal variability of voriconazole PK within individuals and identify predictive clinical factors. This study was conducted as a part of a single‐institution, phase I study of intravenous voriconazole in children undergoing hematopoietic cell transplantation (NCT02227797). We analyzed voriconazole PK study data collected at week 1 and again at week 2 after the start of voriconazole therapy in 59 pediatric patients undergoing HCT (age <21 years). Population PK analysis using nonlinear mixed effect modeling was performed to analyze temporal within‐individual variability of voriconazole PK by incorporating a between‐occasion variability term in the model. A 2‐compartment linear elimination model incorporating body weight and cytochrome P450 2C19 phenotype described the data. The ratio of individual voriconazole clearance between weeks 1 and 2 ranged from 0.11 to 3.3 (−9.1 to +3.3‐fold change). Incorporation of covariate effects by serum C‐reactive protein and albumin levels decreased between‐occasion variability of clearance as compared to the model without them (coefficient of variation, 41.2% and 59.5%, respectively) and improved the model fit (P < .05). As significant covariates on voriconazole PK, C‐reactive protein and albumin concentrations may potentially serve as useful biomarkers as part of therapeutic drug monitoring.
From Fear to Safety: A Roadmap to Recovery from Musculoskeletal Pain.

Contemporary conceptualizations of pain emphasize its protective function. The meaning assigned to pain drives cognitive, emotional, and behavioral responses. When pain is threatening, and a person lacks control over their pain experience, it can become distressing, self-perpetuating, and disabling. Although the pathway to disability is well established, the pathway to recovery is less researched and understood. This Perspective draws on recent data on the lived experience of people with pain-related fear to discuss both fear and safety learning processes and their implications for recovery for people living with pain. Recovery is here defined as achievement of control over pain, as well as improvement in functional capacity and quality of life. Based on the common-sense model, this Perspective proposes a framework utilizing cognitive functional therapy to promote safety learning. A process is described in which experiential learning combined with "sense making" disrupts a person's unhelpful cognitive representation and behavioral and emotional response to pain, leading them on a journey to recovery. This framework incorporates principles of inhibitory processing that are fundamental to pain-related fear and safety learning.
Diagnostic rate of autism spectrum disorder in a high‐survival cohort of children born very preterm: A cross‐sectional study

To investigate the diagnostic rate of autism spectrum disorder (ASD) in a high‐survival cohort of very preterm children, 77 infants born very preterm (<32 weeks of gestation) were assessed at age 4–6 years old using the Autism Diagnostic Observation Schedule, 2nd edition (ADOS‐2) and the Diagnostic and Statistical Manual of Mental Disorders, 5th edition (DSM‐5). Sixteen children (20.8%) were classified as both DSM‐5‐positive ASD and ADOS‐2‐identified “autism,” which were defined as confirmed ASD in this study. Our result suggests that the prevalence of ASD in very preterm children might be much higher than reported in previous studies when all children were individually evaluated. Further studies in a large sample are required to clarify the true risk of ASD in preterm birth.
Brisk walking can be a maximal effort in heart failure patients: a comparison of cardiopulmonary exercise and 6 min walking test cardiorespiratory data

Cardiopulmonary exercise test (CPET) and 6 min walking test (6MWT) are frequently used in heart failure (HF). CPET is a maximal exercise, whereas 6MWT is a self‐selected constant load test usually considered a submaximal, and therefore safer, exercise, but this has not been tested previously. The aim of this study was to compare the cardiorespiratory parameters collected during CPET and 6MWT in a large group of healthy subjects and patients with HF of different severity.
Perioperative risk factors for long‐term intelligence in children with postoperative cerebellar mutism syndrome after medulloblastoma surgery

Approximately 7%–50% of children with medulloblastoma (MB) develop postoperative cerebellar mutism syndrome (pCMS). pCMS has a short‐term negative impact on intelligence, but effects on long‐term outcomes are contradictory. The aim of this study was to assess long‐term effects of pCMS in MB patients on aspects of intelligence quotient (IQ) and its perioperative risk factors.
Reduced expression of hematopoietic progenitor kinase 1 in T follicular helper cells causes autoimmunity of systemic lupus erythematosus

Backgroud T follicular helper (Tfh) cells have been discovered to be the main CD4+ T cells assisting B cells to produce antibody. They are over activated in patients with systemic lupus erythematosus (SLE) and consequently lead to excessive immunity. Hematopoietic progenitor kinase 1 (HPK1) negatively regulates T cell-mediated immune responses and TCR signal. This study aimed to investigate the roles of HPK1 in SLE Tfh cells. Methods HPK1 mRNA and protein levels in Tfh cells were measured by real-time quantitative PCR and western blot analysis, respectively. The production of IL-21, B cell−activating factor (BAFF), interferon γ (IFNγ), IL-17A, IgM, IgG1, IgG2, and IgG3 were analyzed using enzyme linked immunosorbent assay. Tfh cells proliferation was evaluated with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results HPK1 mRNA and protein levels were significantly reduced in SLE Tfh cells, and negatively correlated with SLE disease activity index (SLEDAI) and Systemic Lupus International Collaborating Clinics/American College of Rheumatology (SLICC/ACR) Damage Index for SLE (SDI). Knocking down HPK1 with siRNA in normal Tfh cells greatly elevated Tfh cells proliferation and secretions of IL-21, BAFF, IFNγ, IgG1, IgG2, and IgG3. There were no marked alterations in IL-17A and IgM productions. The opposite effects were observed in SLE Tfh cells transfected with HPK1 overexpressing plasmid: Tfh cells proliferation and productions of IL-21, BAFF, IFNγ, IgG1, IgG2, and IgG3 were all alleviated. And there were no significant changes in IL-17A and IgM levels. Conclusion Our results suggest for the first time that inhibited expression of HPK1 in SLE Tfh cells leading to Tfh cells overactivation and B cells overstimulation, subsequently, the onset and progression of SLE.
A randomised controlled trial to evaluate a medication monitoring system for TB treatment

BACKGROUND : Adherence to TB treatment and therefore treatment success could be improved using digital adherence technology. OBJECTIVE : To evaluate the effectiveness of a medication event reminder monitor system (MERM) on treatment success and treatment adherence in patients with drug-susceptible pulmonary TB in Perú. METHODS : This was an experimental, randomised, open-label, controlled study conducted among patients in the second phase of TB treatment. The intervention group received their medications through MERM with the support of a treatment monitor, whereas the control group used the usual strategy. Participants were followed until they completed the 54 doses of the second phase of treatment. RESULTS : The study included 53 patients in each group; four in the intervention group withdrew from the study. Treatment success was significantly more frequent in the MERM group (RR 1.15, 95% CI 1.02–1.30; P = 0.0322). There was no significant difference in the adherence outcomes; however, the percentage of patients who missed at least one dose and patients with more than 10% of total doses missed were lower in the intervention group. CONCLUSION : The use of MERM in the second phase of treatment showed a significant improvement in the treatment success rate in patients with drug-susceptible pulmonary TB.