Miracle-dz/UniFAR-FTUs huggingface dataset:
Detection of the Tip of the Red Giant Branch in NGC 3379 (M105) in the Leo I Group Using the Hubble Space Telescope

We report the detection of individually resolved stars in the elliptical galaxy NGC 3379, a luminous member of the Leo I Group. The bright end of the stellar luminosity function has a logarithmic slope that is consistent with these stars being Population II red giants. An abrupt discontinuity in the apparent luminosity function at I = 26.30 ± 0.09 mag is identified with the tip of the first-ascent red giant branch (TRGB). Adopting MI(TRGB) = -4.0 ± 0.1 mag gives a distance modulus of 30.30 mag ± 0.14 (random errors) ± 0.23 (systematic errors) corresponding to a linear distance to NGC 3379 of 11.5 ± 1.6 Mpc. The TRGB distance compares very well with the Cepheid distance of 11.9 ± 0.9 Mpc (30.37 ± 0.16 mag) to another group member M96 (=NGC 3368). The distance to NGC 3379 can be used in turn to calibrate the zero points of four other distance indicators: surface brightness fluctuations, planetary nebula luminosity functions, globular cluster luminosity functions and the Dn - σ method. We apply two approaches to measuring the Hubble constant: (1) using a simple Virgocentric infall model and (2) stepping out from Leo I to the Coma cluster using the previously measured relative distance between the two clusters. These give values of the Hubble constant in the range H0 = 60-68 km s-1 Mpc-1, each having a 20% uncertainty. The largest systematic errors could potentially increase this value to H0 = 74 ± 14 km s-1 Mpc-1.
Spatial distribution of sexual network locations used by long-distance truck drivers along the Northern Corridor highway, Kenya

Abstract Background In Kenya, long-distance truck drivers (LDTDs) using the Northern Corridor highway have a high prevalence of HIV and other sexually transmitted infections (STIs) due to their risky sexual networks. However, the spatial distribution of the sexual network locations used by LDTDs is not well understood. Consequently, healthcare stakeholders have found it difficult to provide spatially targeted HIV/STI interventions among LDTDs. Thus, the study sought to establish the spatial distribution of sexual network locations used by LDTDs along the Northern Corridor highway, to inform efficient distribution and use of limited HIV/STI-prevention resources. Methods A cross-sectional study design was used. The study adopted a systematic sampling technique. 296 LDTDs were interviewed using interviewer-administered questionnaires at the Mlolongo weighbridge in Kenya. The LDTDs listed their history of sexual interactions and highway stopovers used during the week preceding data collection. Geospatial modelling techniques, using R statistical software packages for spatial mapping, were employed. Shapefiles were created and overlaid over a map of Kenya using R statistical software to create maps of sexual networks. Results Forty-two highway stopovers used by LDTDs were spatially distributed along the highway, from the Kenya coast to the Kenya–Uganda border. In general, LDTDs’ sexual network hotspots were restricted to the outskirts of major cities along the Northern Corridor highway (Nairobi, Mombasa and Nakuru) as well as the Kenya–Uganda international border. Conclusions On the Northern Corridor highway, stopovers situated proximal to major urban areas, as well as those at international border points, frequently serve as sexual network hotspots among LDTDs and their sexual partners. Thus, healthcare stakeholders should improve access to HIV/STI-prevention services targeted for LDTDs at the sexual network hotspots identified in this study.
Prevalence of self-medication in Ghana: a systematic review and meta-analysis

Objectives This study estimates the prevalence of self-medication and provides an understanding of the reasons for self-medication in Ghana through the synthesis of relevant literature. Methods A comprehensive search was conducted in PubMed, Science Direct and African Journals Online to identify observational studies published from inception to March 2022. Google scholar and institutional websites were searched for grey literature. We included studies reporting primary data on the prevalence and/or reasons for self-medication in Ghana. Random-effects meta-analysis was used to estimate the prevalence of self-medication. Subgroup analysis was performed with the study population (pregnant women, patients and students), geopolitical zone (coastal, middle and northern) and study setting (rural and urban). Using inductive thematic analysis, reasons for self-medication were classified and tallied under key themes. Results Thirty studies involving 9271 participants were included in this review. The pooled prevalence of self-medication in Ghana was 53.7% (95% CI 46.2% to 61.0%; I²=98.51%, p<0.001). Prevalence of self-medication was highest among pregnant women (65.5%; 95% CI 58.1% to 72.5%; I2=88%), in the middle belt of the country (62.1%; 95% CI 40.9% to 82.0%; I²=98%; p<0.001) and in rural settings (61.2%; 95% CI 36.5% to 84.5%; I²=98%; p<0.001). The most cited reasons for self-medication included long waiting time at health facilities (73.3%), previous use of drugs (66.7%) and the perceived unseriousness of diseases (53.3%). Conclusion This study has revealed that self-medication is still an unresolved public health challenge in Ghana, with a high prevalence estimate. Self-medication is influenced by inconveniences associated with accessing healthcare coupled with poor health seeking behaviours. There is the need for improved access to quality healthcare and the promotion of appropriate health-seeking behaviours.
Deciding While Acting—Mid-Movement Decisions Are More Strongly Affected by Action Probability than Reward Amount

Abstract When deciding while acting, such as sequentially selecting targets during naturalistic foraging, movement trajectories reveal the dynamics of the unfolding decision process. Ongoing and planned actions may impact decisions in these situations in addition to expected reward outcomes. Here, we test how strongly humans weigh and how fast they integrate individual constituents of expected value, namely the prior probability (PROB) of an action and the prior expected reward amount (AMNT) associated with an action, when deciding based on the combination of both together during an ongoing movement. Unlike other decision-making studies, we focus on PROB and AMNT priors, and not final evidence, in that correct actions were either instructed or could be chosen freely. This means, there was no decision-making under risk. We show that both priors gradually influence movement trajectories already before mid-movement instructions of the correct target and bias free-choice behavior. These effects were consistently stronger for PROB compared with AMNT priors. Participants biased their movements toward a high-PROB target, committed to it faster when instructed or freely chosen, and chose it more frequently even when it was associated with a lower AMNT prior than the alternative option. Despite these differences in effect magnitude, the time course of the effect of both priors on movement direction was highly similar. We conclude that prior action probability, and hence the associated possibility to plan actions accordingly, has higher behavioral relevance than prior action value for decisions that are expressed by adjusting already ongoing movements.
Brain functional characterization of response-code conflict in dual-tasking and its modulation by age

Crosstalk between conflicting response codes contributes to interference in dual-tasking, an effect exacerbated in advanced age. Here, we investigated (1) brain activity correlates of such response-code conflicts, (2) activity modulations by individual dual-task performance and related cognitive abilities, (3) task-modulated connectivity within the task network, and (4)age-related differences in all these aspects. Young and older adults underwent fMRI while responding to the pitch of tones through spatially mapped speeded button presses with one or two hands concurrently. Using opposing stimulus–response mappings between hands, we induced conflict between simultaneously activated response codes. These response-code conflicts elicited activation in key regions of the multiple-demand network. Older adults showed non-compensatory hyperactivity in left superior frontal gyrus, and higher left intraparietal sulcus activity associated with lower attentional performance. While motor and parietal areas of the conflict-related network were modulated by attentional and task-switching abilities, efficient conflict resolution in dual-tasking was linked to suppressing visual cortex activity. Finally, connectivity between premotor or parietal seed regions and the conflict-sensitive network was neither conflict-specific nor age-sensitive. Overall, resolving dual-task response-code conflict recruited substantial parts of the multiple-demand network, whose activity and coupling, however, were only little affected by individual differences in task performance or age.
Establishment and validation of a High-throughput Micro-Neutralization assay for Respiratory Syncytial Virus (subtypes A and B)

The validation of a bioanalytical method allows us to determine its validity for a designated purpose and to guarantee the reliability of its analytical results. The virus neutralization assay has proved to be suitable for the detection and quantization of specific serum neutralizing antibodies against respiratory syncytial virus subtypes A and B. Respiratory syncytial virus is a negative-sense RNA virus and is responsible for the majority of acute lower respiratory tract infections in infants and older adults worldwide. Owing to its widespread infection, the WHO considers it a target for the development of preventive vaccines. Despite the high impact of its infections, however, no vaccine candidate is currently available. The aim of this paper is to provide a detailed validation process for the micro-neutralization assay and to demonstrate that this method can effectively support the efficacy assessment of candidate vaccines and the definition of correlates of protection.
Inhibition of CDK12 elevates cancer cell dependence on P-TEFb by stimulation of RNA polymerase II pause release

P-TEFb and CDK12 facilitate transcriptional elongation by RNA polymerase II. Given the prominence of both kinases in cancer, gaining a better understanding of their interplay could inform the design of novel anti-cancer strategies. While down-regulation of DNA repair genes in CDK12-targeted cancer cells is being explored therapeutically, little is known about mechanisms and significance of transcriptional induction upon inhibition of CDK12. We show that selective targeting of CDK12 in colon cancer-derived cells activates P-TEFb via its release from the inhibitory 7SK snRNP. In turn, P-TEFb stimulates Pol II pause release at thousands of genes, most of which become newly dependent on P-TEFb. Amongst the induced genes are those stimulated by hallmark pathways in cancer, including p53 and NF-κB. Consequently, CDK12-inhibited cancer cells exhibit hypersensitivity to inhibitors of P-TEFb. While blocking P-TEFb triggers their apoptosis in a p53-dependent manner, it impedes cell proliferation irrespective of p53 by preventing induction of genes downstream of the DNA damage-induced NF-κB signaling. In summary, stimulation of Pol II pause release at the signal-responsive genes underlies the functional dependence of CDK12-inhibited cancer cells on P-TEFb. Our study establishes the mechanistic underpinning for combinatorial targeting of CDK12 with either P-TEFb or the induced oncogenic pathways in cancer.
Turbulence and blood washout in presence of mitral regurgitation: a computational fluid-dynamics study in the complete left heart

In this work we performed a computational image-based study of blood dynamics in the whole left heart, both in a healthy subject and in a patient with mitral valve regurgitation (MVR). We elaborated dynamic cine-MRI images with the aim of reconstructing the geometry and the corresponding motion of left ventricle, left atrium, mitral and aortic valves, and aortic root of the subjects. This allowed us to prescribe such motion to computational blood dynamics simulations where, for the first time, the whole left heart motion of the subject is considered, allowing us to obtain reliable subject-specific information. The final aim is to investigate and compare between the subjects the occurrence of turbulence and the risk of hemolysis and of thrombi formation. In particular, we modeled blood with the Navier-Stokes equations in the Arbitrary Lagrangian-Eulerian framework, with a Large Eddy Simulation model to describe the transition to turbulence and a resistive method to manage the valve dynamics, and we used a Finite Elements discretization implemented in an in-house code for the numerical solution. Our results highlighted that the regurgitant jet in the MVR case gave rise to a large amount of transition to turbulence in the left atrium resulting in a higher risk of formation of hemolysis. Moreover, MVR promoted a more complete washout of stagnant fiows in the left atrium during the systolic phase and in the left ventricle apex during diastole. NEW & NOTEWORTHY Reconstruction from cine-MRI images of geometries and motion of the left heart (left atrium and ventricle, aortic root, aortic and mitral valve) of a healthy and mitral regurgitant patient. Prescription of such motion to a complete subject-specific computational fluid-dynamic simulation of the left heart. Investigation of turbulence in a regurgitant scenario. Study of the mechanisms of prevention from stagnant flows and hemolysis formation in the atrium.
Comparative transcriptome analysis in contrasting finger millet (Eleusine coracana (L.) Gaertn) genotypes for heat stress

Eleusine coracana (L.) Gaertn is a crucial C4 species renowned for its stress robustness and nutritional significance. Because of its adaptability traits, finger millet (ragi) is a storehouse of critical genomic resources for crop improvement. However, more knowledge about this crop’s molecular responses to heat stress must be gained. Hence, in the present study, we generated RNA seq data from the leaf tissue of the finger millet to observe the physiological changes and gene expression study in heat-sensitive (KJNS-46) and heat-tolerant (PES-110) genotypes of Ragi in response to high temperatures. On average, each sample generated about 24 million reads. Nearly 684 transcripts were differentially expressed (DEGs) between the heat-stressed samples of both genotypes. Pathway analysis and functional annotation analysis revealed the activation of various genes involved in response to stress, precisely heat, oxidation-reduction process, water deprivation, heat shock protein and transcription factors, calcium, and kinase signaling. The basal regulatory genes, such as bZIP, were involved in response to heat stress, indicating that heat stress activates genes related to basal regulatory processes or housekeeping. A substantial percentage of the DEGs belonged to proteins of unknown functions (PUFs), i.e., uncharacterized. The expression pattern of a few selected DEGs genes was analyzed in both genotypes by quantitative RT-PCR. The present study found some candidate genes and pathways that may confer tolerance to heat stress in ragi. These results will provide valuable information to improve heat tolerance in heat-susceptible agronomically important varieties of ragi and other crop plants.
Sustained in vivo perfusion of a re-endothelialized tissue engineered kidney graft in a human-scale animal model

Introduction Despite progress in whole-organ decellularization and recellularization, maintaining long-term perfusion in vivo remains a hurdle to realizing clinical translation of bioengineered kidney grafts. The objectives for the present study were to define a threshold glucose consumption rate (GCR) that could be used to predict in vivo graft hemocompatibility and utilize this threshold to assess the in vivo performance of clinically relevant decellularized porcine kidney grafts recellularized with human umbilical vein endothelial cells (HUVECs). Materials and Methods Twenty-two porcine kidneys were decellularized and 19 were re-endothelialized using HUVECs. Functional revascularization of control decellularized (n=3) and re-endothelialized porcine kidneys (n=16) was tested using an ex vivo porcine blood flow model to define an appropriate metabolic glucose consumption rate (GCR) threshold above which would sustain patent blood flow. Re-endothelialized grafts (n=9) were then transplanted into immunosuppressed pigs with perfusion measured using angiography post-implant and on days 3 and 7 with 3 native kidneys used as controls. Patent recellularized kidney grafts underwent histological analysis following explant. Results The glucose consumption rate of recellularized kidney grafts reached a peak of 41.3±10.2 mg/hour at 21±5 days, at which point the grafts were determined to have sufficient histological vascular coverage with endothelial cells. Based on these results, a minimum glucose consumption rate threshold of 20 mg/hour was set. The revascularized kidneys had a mean perfusion percentage of 87.7±10.3%, 80.9±33.1%, and 68.5±38.6% post-reperfusion on Days 0, 3 and 7, respectively. The 3 native kidneys had a mean post-perfusion percentage of 98.4±1.6%. These results were not statistically significant. Conclusion This study is the first to demonstrate that human-scale bioengineered porcine kidney grafts developed via perfusion decellularization and subsequent re-endothelialization using HUVEC can maintained patency with consistent blood flow for up to 7 days in vivo. These results lay the foundation for future research to produce human-scale recellularized kidney grafts for transplantation.
Asymmetries in strong-field photoionization by few-cycle laser pulses: Kinetic-energy spectra and semiclassical explanation of the asymmetries of fast and slow electrons

Using numerical solutions of a time-dependent Schroedinger equation for a hydrogen atom in a linearly polarized few-cycle laser field, we calculate the left-right photoelectron kinetic-energy spectra measured by two opposing detectors placed along the laser polarization vector, with laser focus in the center. The fastest electrons show huge asymmetries strongly dependent on the laser carrier-envelope (CE) phase which confirms the recent theoretical results [D. B. Milosevic et al., Opt. Express 11, 1418 (2003)], obtained from a modified strong field approximation model which includes rescattering by the Coulomb potential. This asymmetry can also be explained by a simple semiclassical model in which the electron after tunneling through a potential barrier returns to the proton and is elastically backscattered in the presence of the laser field thus acquiring energy close 10U{sub p} where U{sub p} is the electron ponderomotive energy in the laser field. We also present a semiclassical interpretation of counterintuitive left-right asymmetries of slow electrons discussed in our previous work [Phys. Rev. A. 70, 013815 (2004)]. Our analysis shows that the Coulomb attraction from the proton must be included in the standard tunneling model in order to account for the CE phase dependent angular asymmetry seen in our previous numericalmore » calculations. « less
Explicit bounds on exponential sums and the scarcity of squarefree binomial coefficients

The distribution of squarefree binomial coefficients . For many years, Paul Erdős has asked intriguing questions concerning the prime divisors of binomial coefficients, and the powers to which they appear. It is evident that, if k is not too small, then must be highly composite in that it contains many prime factors and often to high powers. It is therefore of interest to enquire as to how infrequently is squarefree. One well-known conjecture, due to Erdős, is that is not squarefree once n > 4. Sarkozy [Sz] proved this for sufficiently large n but here we return to and solve the original question.
Using Logistic Regression to Characterise Communal Cattle Farmers in Botswana

This paper aims to characterise and profile subsistence cattle farmers according to selected attributes to establish their influence on the type of cattle breed kept by farmers in Botswana. The development of communal cattle production can be a sustainable way to improve the livelihoods of the rural population in Botswana. However, there needs to be more information or research conducted to characterise and profile communal cattle farmers with a precondition that the farmers can keep any of the three breeds (Tswana, Cross, or Exotic). A logistic regression model was fitted to determine the influence of 11 predictor variables on the type of cattle breed kept by the farmers. Results revealed that female-headed households were 50% more likely to have the Tswana breed of cattle than male-headed households. In contrast, female-headed households were 30% less likely to have cross-breed or exotic-breed cattle than male-headed households. Results further show that resource-poor farmers tend to keep Tswana breed cattle. These are holdings with no farm labour, no other economic activities, female-headed households, and their primary source of income specified as “other”.
Current options for the treatment of advanced or metastatic NSCLC

Non-small cell lung cancer (NSCLC) remains the predominate cause of cancer-related death worldwide. The estimate in the last decade indicates that for patients with metastatic NSCLC, the overall 5-year survival rate is only 7%. It is worthy that molecular target therapies or immunotherapy enable patients with metastatic lung cancer to prolong overall survival and associated with less frequent adverse events. However, chemotherapy alone, such as docetaxel, is correlated with limited efficacy and inevitable toxicity. Although cytotoxic chemotherapy still plays a vital role in cancer interventions, the biomarker-directed therapies that target the molecular alterations or programmed cell death 1 (PD-L1) and programmed cell ligand 1 (PD-1) pathways have substituted for chemotherapy as the standard first-line or second-line therapies for nearly half of patients with advanced NSCLC. An identifiable biomarker that predicts the efficacy of immune checkpoint blockades and tyrosine kinase inhibitors (TKIs) is necessary to determine the patients who most probably benefit from immunotherapy. Evaluating the PD-L1 expression level and workable molecular alterations may be the best prediction indicator for efficacy in NSCLC. This review summarizes the effectiveness in current clinical trials guiding the patients with NSCLC using checkpoint blockades and TKIs as first-line treatment, evaluating the survival efficacy of PD-L1 expression level in selected patients treated with checkpoint blockades. Sixty-three articles about clinical trials of immunotherapy for NSCLC were identified from PubMed. All included articles were published from January 1, 2007, to January 30, 2021, and divided into different PD-L1 expression levels to analyze survival.