Miracle-dz/UniFAR-FTUs huggingface dataset:
ABO blood group antigens in patients with psoriasis and pemphigus vulgaris

Background: The ABO blood group antigens play a role in the pathophysiology of some diseases and several researches have investigated this relationship in the field of dermatology. In the present study, an attempt was made to find the distribution of blood types in patients with pemphigus vulgaris and chronic plaque type psoriasis. Method: Fifty patients with chronic plaque type psoriasis, 50 patients with pemphigus vulgaris and 100 healthy persons as control group, were selected as the study population. ABO blood grouping was determined by the cell type tube test method, using standard commercial anti/A, anti/B and 5% suspension of red blood cells. Chi square test was used to compare the frequency of ABO blood groups in patients and control groups. Result: Among the 50 patients with psoriasis, 21 (42%) had blood group O, 15 (30%) blood group A, 10 (20%) blood group B and 8 (4%) had blood group AB. Among the 50 patients in the pemphigus group, 22 (44%) had blood group O, 19 (38%) blood group A, 8 (16%) blood group B and 1 (2%) had blood group AB. The distribution of blood groups in patients with these two diseases was not significantly different from the control group.
Biochemical Markers in Alzheimer’s Disease

Alzheimer’s disease (AD) is one of the most frequent neurodegenerative diseases affecting more than 35 million people in the world, and its incidence is estimated to triple by 2050. Alzheimer’s disease is an age-related disease characterized by the progressive loss of memory and cognitive function, caused by the unstoppable neurodegeneration and brain atrophy. Current AD treatments only relieve the symptoms. The first molecular signs of the disease identified decades ago and were related to the tau neurofibrillary tangles and the β amyloid plaques. Despite the considerable progress in the diagnostic field, there is no certain knowledge of the specific biomarkers reflecting molecular mechanisms that trigger the symptoms of the disease. Therefore, there is an enormous need to find biomarkers useful for early diagnosis, before the first symptoms appear, and develop new therapeutic targets, which would guarantee improving patients’ quality of life. Researchers from all around the world are looking for biomarkers that can be identified in different biological fluids such as plasma, serum, and cerebrospinal fluid, specific for Alzheimer’s disease. In this review, we would like to resume some of the most interesting discovery in pathological mechanisms underlying Alzheimer’s disease and promising biomarkers.
Evaluation of DMFT in paediatric patients with social vulnerability conditions.

AIM The aim of this study was to analyse the dmft/DMFT index in paediatric patients belonging to families with low income, in conditions of social vulnerability and absolute poverty and to compare it with a control group with a good socioeconomic status. MATERIALSN AND METHODS The study analysed a total sample of 160 patients with average age of 8.6±2.5. The sample was divided into two groups based on the Equivalent Economic Situation Indicator (ISEE). Group 1 consists of 80 patients with an ISEE value less than €6.000 and was examined at the "Solidarietà Vincenziana" Dental Centre - Rome (Italy), which is a centre dedicated to people with minimum income, destitute, elderly without resources, immigrant children; Group 2 consists of 80 patients with an ISEE value of more than €20.000 and was examined at the Pediatric Dentistry Unit, University of Rome Tor Vergata. STATISTICS Statistical analysis was performed using SPSS for Windows version 21 (IBM SPSS Inc., Chicago, IL, USA). The statistical analysis included a descriptive evaluation of the results in a bivariate analysis. The association between the presence of caries and the background variables was evaluated with the chi-squared association or Fisher test. The minimum level of significance was fixed at P-value? 0.05. RESULTS Analysing the number of caries-free subjects and subjects with caries in reference to the age group between 5 and 12 years and the ISEE value, without distinction of sex, there is a statistically significant difference between Group 1 and Group 2, both in relation to the dmft (p=0.038, Chi-squared Test=4.28) and to the DMFT (p=0.001, Chi-squared Test=19.23). Subjects aged between 5 and 12 years had an average DMFT of 1.88 ± 0.83 (Group 1) and 0.95 ±0.54 (Group 2). CONCLUSIONS The study highlights a positive relationship between ISEE value, of poor socio-economic situations (condition of absolute poverty, low economic income) and increase in the DMFT index. The oral health status is an indicator of poverty. For this reason it would be advisable to plan early preventive interventions, providing the possibility of appropriate and effective access for children in economic and social needs, whose quality of life can be further negatively affected by oral diseases.
Quantifying rapidly declining abundance of insects in Europe using a paired experimental design

Abstract The abundance of insects has decreased for the last decades in many parts of the world although so far few studies have quantified this reduction because there have only been few baseline studies dating back decades that have allowed comparison of ancient and recent population estimates. Such a paired design is particularly powerful because it reduces or eliminates bias caused by differences in identity and experience of observers, identity of study sites, years, time of season, and time of day, and it ensures identity of sampling procedures. Here, I compiled information on the reduction in abundance of insects in Europe and Algeria by the same persons compiling the abundance of insects from the same 21 study sites during 1951–1997 and again a second time in 1998–2018. There was a reduction by 47% in the abundance of insects. The difference in abundance in old compared to new samples declined with latitude, with a significant variance among taxa. This reduction in abundance of insects was of such a magnitude that it must have consequences for insectivores and the role that insects play in ecosystems.
Imported Arbovirus Infections in Spain, 2009–2018

To determine the epidemiologic and clinical characteristics of patients in Spain with imported arbovirus infections, we analyzed 22,655 records from a collaborative network for January 2009–December 2018. Among 861 arbovirus infections, 845 were monoinfections (456 [53%] dengue, 280 [32.5%] chikungunya, 109 [12.7%] Zika) and 16 (1.8%) were co-infections. Most patients were travelers (56.3%) or immigrants returning to Spain after visiting friends or relatives (31.3%). Median patient age was 37 years; most (62.3%) were women and some (28.6%) had received pretravel advice. Only 12 patients were immunosuppressed. Six cases (all dengue monoinfections, none in immunosuppressed patients) were severe. Since 2014, nondengue arbovirus infections increased; until 2016, chikungunya and Zika were most common. Imported arbovirus infections (mostly dengue) were frequently diagnosed, although increased chikungunya and Zika virus infections coincided with their introduction and spread in the Americas. A large proportion of cases occurred in women of childbearing age, some despite receipt of pretravel advice.
Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium

Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibacterium genus or even the Pasteurellaceae family, such as aadA23, blaCARB-8, tet(Y), and qnrD1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential.
The actin comet guides the way: How Listeria actin subversion has impacted cell biology, infection biology and structural biology

The discovery of the role of ActA to polymerise actin at one pole of Listeria monocytogenes represents a key event in the field of cellular microbiology. It uncovered much more than the molecular principle behind actin‐based motility of Listeria within the cytosol of infected cells, and it changed the way how actin dynamics could be studied and eventually understood. The ActA discovery took place at a time when cell biology, biochemistry and microbiology came together in a very fruitful fashion. Here, we provide an overview of the science that took place around this event. Then, we outline the wide array of research fields that have been impacted by this finding. This ranges from structural and biophysical investigations on actin and its dynamics, the role of actin polymerisation during infection with different pathogens, to actin‐dynamics during various pathologies. Like a comet in the sky, Pascale Cossart's work on ActA has inspired and will inspire generations of (life) scientists.
No Longer Underappreciated: The Emerging Concept of Astrocyte Heterogeneity in Neuroscience

Astrocytes are ubiquitous in the central nervous system (CNS). These cells possess thousands of individual processes, which extend out into the neuropil, interacting with neurons, other glia and blood vessels. Paralleling the wide diversity of their interactions, astrocytes have been reported to play key roles in supporting CNS structure, metabolism, blood-brain-barrier formation and control of vascular blood flow, axon guidance, synapse formation and modulation of synaptic transmission. Traditionally, astrocytes have been studied as a homogenous group of cells. However, recent studies have uncovered a surprising degree of heterogeneity in their development and function, in both the healthy and diseased brain. A better understanding of astrocyte heterogeneity is urgently needed to understand normal brain function, as well as the role of astrocytes in response to injury and disease.
Mutations in calmodulin-binding domains of TRPV4/6 channels confer invasive properties to colon adenocarcinoma cells

ABSTRACT Transient receptor potential (TRP) channels form a family of polymodal cation channels gated by thermal, mechanical, or chemical stimuli, with many of them involved in the control of proliferation, apoptosis, or cell cycle. From an evolutionary point of view, TRP family is characterized by high conservation of duplicated genes originating from whole-genome duplication at the onset of vertebrates. The conservation of such “ohnolog” genes is theoretically linked to an increased probability of generating phenotypes deleterious for the organism upon gene mutation. We aimed to test experimentally the hypothesis that TRP mutations, in particular gain-of-function, could be involved in the generation of deleterious phenotypes involved in cancer, such as gain of invasiveness. Indeed, a number of TRP channels have been linked to cancer progression, and exhibit changes in expression levels in various types of cancers. However, TRP mutations in cancer have been poorly documented. We focused on 2 TRPV family members, TRPV4 and TRPV6, and studied the effect of putative gain-of-function mutations on invasiveness properties. TRPV channels have a C-terminal calmodulin-binding domain (CaMBD) that has important functions for regulating protein function, through different mechanisms depending on the channel (channel inactivation/potentiation, cytoskeleton regulation). We studied the effect of mutations mimicking constitutive phosphorylation in TRPV4 and TRPV6 CaMBDs: TRPV4 S823D, S824D and T813D, TRPV6 S691D, S692D and T702. We found that most of these mutants induced a strong gain of invasiveness of colon adenocarcinoma SW480 cells, both for TRPV4 and TRPV6. While increased invasion with TRPV6 S692D and T702D mutants was correlated to increased mutant channel activity, it was not the case for TRPV4 mutants, suggesting different mechanisms with the same global effect of gain in deleterious phenotype. This highlights the potential importance to search for TRP mutations involved in cancer.
Reduced egocentric bias when perspective-taking compared with working from rules

Previous research has suggested that adults are sometimes egocentric, erroneously attributing their current beliefs, perspectives, and opinions to others. Interestingly, this egocentricity is sometimes stronger when perspective-taking than when working from functionally identical but non-perspectival rules. Much of our knowledge of egocentric bias comes from Level 1 perspective-taking (e.g., judging whether something is seen) and judgements made about narrated characters or avatars rather than truly social stimuli such as another person in the same room. We tested whether adults would be egocentric on a Level 2 perspective-taking task (judging how something appears), in which they were instructed to indicate on a continuous colour scale the colour of an object as seen through a filter. In our first experiment, we manipulated the participants’ knowledge of the object’s true colour. We also asked participants to judge either what the filtered colour looked like to themselves or to another person present in the room. We found participants’ judgements did not vary across conditions. In a second experiment, we instead manipulated how much participants knew about the object’s colour when it was filtered. We found that participants were biased towards the true colour of the object when making judgements about targets they could not see relative to targets they could, but that this bias disappeared when the instruction was to imagine what the object looked like to another person. We interpret these findings as indicative of reduced egocentricity when considering other people’s experiences of events relative to considering functionally identical but abstract rules.
Relationship between molar deciduous teeth infraocclusion and mandibular growth: A case-control study.

AIM As the effects of infraocclusion on mandibular growth could have a significant impact on the treatment planning made by the orthodontist, the aim of this study is to evaluate the correlation between infraocclusion and mandibular growth. MATERIALS AND METHODS Study design: A total of 42 healthy Caucasian patients with no history of dental trauma or orthodontic treatment were selected for this study from the same paediatric dentistry department. These patients were divided in two groups based on the presence or absence of infraocclusion: Group A, 22 patients (12 males and 10 females) suffering from infraocclusion of at least one inferior deciduous molar, and Group B, 20 patients (9 males and 11 females) who did not suffer from infraocclusion but had to be treated for dental overcrowding. Each patient was examined through an orthopantomography scan and lateral cephalogram. All measurements were made before the start of the treatment, and they were analysed in order to show cephalometric correlation between the skeletal class, the vertical dimension and cranio-maxillo-mandibular divergence. RESULTS According to Student's t-test, the only measurements which had a statistically significant difference between Group A and Group B were the SNB angle (p-value <0.01), the ANB angle (p-value <0.05) and the PC-GO-GN angle (p-value <0.05). CONCLUSION From the data collected in this study, it is possible to conclude that patients suffering from infraocclusion of the mandibular deciduous molars show mandibular post-rotation and an increased total Gonial angle, compared to patients who do not suffer from infraocclusion. Despite the absence of a longer-term evaluation of these patients, thanks to this data it is possible to state that early diagnosis of infraocclusion is fundamental in order to apply a well-planned and thought-out orthodontic treatment.
Administrative and Claims Data Help Predict Patient Mortality in Intensive Care Units by Logistic Regression: A Nationwide Database Study

Background Increasing attention has been paid to the predictive power of different prognostic scoring systems for decades. In this study, we compared the abilities of three commonly used scoring systems to predict short-term and long-term mortalities, with the intention of building a better prediction model for critically ill patients. We used the data from the National Health Insurance Research Database (NHIRD) in Taiwan, which included information on patient age, comorbidities, and presence of organ failure to build a new prediction model for short-term and long-term mortalities. Methods We retrospectively collected the medical records of patients in the intensive care unit of a regional hospital in 2012 and linked them to the claims data from the NHIRD. The Acute Physiology and Chronic Health Evaluation II (APACHE II) score, Elixhauser Comorbidity Index (ECI), and Charlson Comorbidity Index (CCI) were compared for their predictive abilities. Multiple logistic regression tests were performed, and the results were presented as receiver operating characteristic curves and C-statistic. Results The APACHE II score has the best predictive power for inhospital mortality (0.79; C − statistic = 0.77 − 0.83) and 1-year mortality (0.77; C − statistic = 0.74 − 0.79). The ECI and CCI alone have poorer predictive power and need to be combined with other variables to be comparable to the APACHE II score, as predictive tools. Using CCI together with age, sex, and whether or not the patient required mechanical ventilation is estimated to have a C-statistic of 0.773 (95% CI 0.744-0.803) for inhospital mortality, 0.782 (95% CI 0.76-0.81) for 30-day mortality, and 0.78 (95% CI 0.75-0.80) for 1-year mortality. Conclusions We present a new prognostic model that combines CCI with age, sex, and mechanical ventilation status and can predict mortality, comparable to the APACHE II score.
Beyond Energy Balance: Environmental Trade-offs of Organics Capture and Low Carbon-to-Nitrogen Ratio Sewage Treatment Systems.

Several life-cycle assessments (LCAs) have evaluated the environmental impacts (EIs) of different wastewater treatment (WWT) configurations, attempting resource recovery and energy efficiency. However, a plant-wide LCA considering up-concentration primary treatment and low carbon-to-nitrogen (C/N) ratio sewage at the secondary biological treatment (SBT) has not yet been conducted. This study identifies the environmental trade-offs and hotspots for the chemically enhanced primary treatment (CEPT) and low C/N ratio SBT emerging processes compared to conventional WWT. The life-cycle inventories were calculated using a stoichiometric life-cycle inventory framework that couples stoichiometry and kinetics to obtain site-specific water, air, and soil emissions. The midpoint results of LCA show that CEPT with anaerobic digestion (AD) for sludge treatment achieves energy self-sufficiency, but increases marine eutrophication (MEu) by one order of magnitude compared to conventional WWT. A mainstream anaerobic fluidized-bed bioreactor and a partial nitritation-anammox fluidized-bed membrane bioreactor which can reduce all environmental impacts by 17%-47%, including MEu, are proposed as the SBT of the low-carbon CEPT settled sewage. Integrating the standardized S-LCI framework resulted in a site-specific LCA that aids decision-makers on choosing between higher reductions in most EIs at the expense of high MEu or less but consistent reductions in all EI categories.
Feasibility of Telephone Follow-Up after Critical Care Discharge

Background: Critical care has evolved from a primary focus on short-term survival, with greater attention being placed on longer-term health care outcomes. It is not known how best to implement follow-up after critical care discharge. Study aims were to (1) assess the uptake and feasibility of telephone follow-up after a critical care stay and (2) profile overall physical status and recovery during the sub-acute recovery period using a telephone follow-up assessment. Methods: Adults who had been admitted to critical care units of St. James’s Hospital, Dublin, for >72 h were followed up by telephone 3–9 months post discharge from critical care. The telephone assessment consisted of a battery of questionnaires (including the SF-36 questionnaire and the Clinical Frailty Scale) and examined quality of life, frailty, employment status, and feasibility of telephone follow-up. Results: Sixty five percent (n = 91) of eligible participants were reachable by telephone. Of these, 80% (n = 73) participated in data collection. Only 7% (n = 5) expressed a preference for face-to-face hospital-based follow-up as opposed to telephone follow-up. For the SF-36, scores were lower in a number of physical health domains as compared to population norms. Frailty increased in 43.2% (n = 32) of participants compared to pre-admission status. Two-thirds (n = 48) reported being >70% physically recovered. Conclusion: Results showed that telephone follow-up is a useful contact method for a typically hard-to-reach population. Deficits in physical health and frailty were noted in the sub-acute period after discharge from critical care.
Psychotherapists’ attitudes to intimate and informal behaviour towards clients

Abstract Background To avoid harming or exploiting a client, sexual and non-sexual dual relationship is generally considered as unacceptable in the psychotherapeutic relationship. However, little is known about what therapists themselves constitute as (un)acceptable intimate and informal behaviour (IIB). Methods A survey among psychotherapists in Flanders (Belgium) was conducted. Opinions about the acceptability of IIB were asked. Based on these opinions attitude groups could be determined. Results In total, 786 therapists completed and returned the questionnaire (response rate: 39.8%). Therapists could be divided into three attitude groups. Almost half of the therapists belonged to the ‘rather restrictive group’, a third to the ‘rather socially permissive group’ and a fifth to the ‘rather sexually permissive group’. Being categorised as ‘rather sexually permissive’ is predominantly related to being male and non-heterosexual, whereas being ‘rather restrictive’ or ‘rather socially permissive’ is mainly due to the type of psychotherapy training. The ‘rather sexually permissive’ therapists more often found a client sexually attractive during the last year and fantasised more often about a romantic relationship with a client, but they did not more often started a sexual relationship. Conclusions Most therapists in Flanders are rather restrictive in their attitude to IIB, pointing to a high sense of morality. Having a rather sexually permissive attitude is predominantly related to more personal characteristics of the therapists, but these therapists did not start a sexual relationship more often.