Miracle-dz/UniFAR-FTUs huggingface dataset:
Chip-Based Digital Pcr Improves the Detection of Low-Rate pik3ca Mutations in Breast Cancer Patients

PIK3CA is a gene usually mutated in breast cancer and has an important role in tumor progression and treatment. Therefore, there is required a technique to detect low-rate PIK3CA mutations improving the clinical conduct. This study aimed to compare chip-based dPCR and Sanger sequencing to detect PIK3CA mutations in breast cancer patients. Fifty-seven tumor samples from breast cancer patients were collected and analyzed by Sanger sequencing and dPCR for PIK3CA mutations (E545K, H1047R, and H1047L). Digital PCR sensitivity, specificity, and overall performance were estimated by contingency tables, receptor operator characteristic (ROC), and area under the curve (AUC). Sanger sequencing identified PIK3CA mutations in six patients (10.5%), two with H1047R, and four with E545K. Digital PCR confirmed those mutations and identified 19 additional patients with at least one mutation. Comparison between dPCR and Sanger sequencing showed a sensitivity of 100% (95% CI 53-100%), and a specificity of 84.2% (95% CI 83 84.2%). Besides, H1047R mutation showed a significant association with breast cancer phenotype (p =0.019) and lymphatic node infiltration (p =0.046). Digital PCR showed a high sensitivity to detect mutations in tumor samples and it might be capable to detect low-rate mutations and tumor subpopulations not detected by Sanger sequencing.
ANALYSIS OF FILTER BANK MULTI CARRIER SYSTEM FOR ADVANCED CELLULAR COMMUNICATION INTELLIGENT HEALTHCARE SYSTEM

Filter Bank Multi Carrier (FBMC) has the drawback of high Peak Average Power Ratio (PAPR) in the cellular communication environment. In multi-carrier techniques, the resulting output is the addition of several subcarriers that leads to a condition where output instantaneous power becomes larger. In this scenario, the system suffers from high PAPR which considerably hampers the performance of the amplifier. It usually occurs when an amplifier used in multi-carrier techniques swings from linear to the non-linear region. PAPR is one of the reason due to which the efficiency of the FBMC-QAM system is reduced. The work deals with the design and implementation of next generation mobile communication systems. Different spectrum detection techniques are used to evaluate the system performance. The performance of the system is explored by plotting bit error rate (BER) Vs Signal to noise ratio (SNR), probability of detection (Pd), for different proposed techniques.
Laboratory Investigations into the Velocities and Attenuation of Ultrasonic Waves in Sand Samples Containing Water/Ice and Methane and Tetrahydrofuran Hydrates

—The paper considers the results of a series of laboratory experiments (more than 100) on the formation of synthetic sand samples containing water/ice and methane or tetrahydrofuran hydrates in the pore space and of the measurement of their acoustic properties (velocities and attenuation of acoustic waves). The main aim of the experiments was to establish the relationship between the velocities of acoustic waves and the ice or hydrate saturation of the samples. An increase in the content of ice and hydrates always leads to a velocity increase. However, the rate of the velocity increase is determined by the localization of ice and hydrates in the samples: at the contacts between the sand grains (“cementing” model) or in the pore space (“filling” model). It has been established that the “cementing” model, characterized by a drastic initial increase in velocities, works for ice or gas hydrates formed from free methane and localized in the pores. On the contrary, tetrahydrofuran hydrates form by the “filling” model and cause a slow increase in velocities.
Alterations associated in dendritic cells sub-types in smokers and non-smokers

Introduction: Dendritic cells (DCs) consider as greatest significance cells of innate and adaptive immune system because they connect both types. These cells are necessary for controlling immunity and tolerance balance. Objective: Effect of cigarette smoking on immunological properties of DCs and their subtypes plasmacytoid DCs and myeloid dendritic cells. Methods: The study included 27 smokers (female and male) and 18 nonsmoker controls. Isolating peripheral blood mononuclear cells, later, counted the total white blood cells (WBCs/mm) by hemocytometer. DCs population, maturation marker CD83, and intracellular cytokines (IL-12, IFNα) were detected using multiparametric flow cytometry analysis. Results: Increasing absolute number of pDCs was noted among smokers in general and among male smokers specifically. Therefore, mDCs/pDCs ratio was decreased in same populations. A comparison of male and female smokers reported that, increasing in the absolute number of pDCs in males. No detectable changes recorded for the expression of CD83 or the intracellular cytokines. Conclusions: Our results support that smoking alters the ratio for ANALYSIS 24(105), September October, 2020 Medical Science ISSN 2321–7359 EISSN 2321–7367
Influence of Return on Assets and Total Assets Turnover on the Systematic Risk of Shares with Price Book Value as Intervening Variables

This study applies a comparative causal method with a quantitative approach in connection with verification analysis with the object of research in the form of issuers sectoral index issuers for Property, Real Estate and Building Construction listed on the Indonesia Stock Exchange during the 2014 2018 period. Whether or not the effect of Return On Asset (ROA) and Total Asset Turnover (TATO) on Systematic Risk (RS) through Price Book Value (PBV) as an intervening variable. The data collection technique applied in this study is to use the library research method in connection with the application of secondary data. The study population was 86 issuers of the sectoral index of Property, Real Estate and Building Construction. In contrast, the research sample of 39 issuers sectoral index property, real estate and building construction was obtained by applying the purposive sampling method. The data analysis technique applied in this study is to use path analysis. Based on the test results using the 5% significance level, the following conclusions are obtained: (1) ROA has a positive and significant effect on PBV (2) TATO has a positive and significant effect on PBV. (3) ROA has a negative and significant effect on RS. (4) TATO has a positive and insignificant effect on RS. (5) PBV has a negative and significant effect on RS. (6) PBV mediates the effect of ROA on RS (7) PBV does not mediate the effect of TATO on RS.
Do Workers Feel Entitled to High Wages? Evidence from a Long-Term Field Experiment

We followed eldworkers administering a household survey over a 12-week period and examined how their reciprocal behavior towards the employer responded to a sequence of exogenous wage increases and wage cuts. To disentangle the e¤ects of reciprocal behavior from other explicit incentives that occur naturally in long-term employment relationships, we devised a novel measure of e¤ort that eldworkers perceived as unmonitored. While wage increases had no signi cant e¤ect, wage cuts back to the initial wage and then below the initial wage led to a strong and persistent decline in unmonitored e¤ort, even though compensation throughout the experiment remained several times higher than the going market wage. The estimates provide clear evidence of a highly asymmetric reciprocity response to wage changes and imply that eldworkers quickly came to feel entitled to higher wages when deciding on reciprocity. Together, these ndings explain why rms rarely cut wages, an empirical phenomenon known as Downward Wage Rigidity. Keywords: Reciprocity; Field experiment; Downward Wage Rigidity JEL Classi cation: C93, J30 We thank Ernst Fehr, Luke Taylor as well as seminar participants at The Wharton School and McGill University for valuable comments. Chemin thanks the Social Sciences and Humanities Research Council of Canada (SSHRC) and the Fonds de Recherche sur la Société et la Culture (FQRSC) for nancial support. Kurmann gratefully acknowledges the hospitality of The Wharton School where part of this project was completed. All views are those of the authors and do not necessarily reect those of the Federal Reserve Board; or any other institution with which the authors are a¢ liated. Contact information: matthieu.chemin@mcgill.ca; andre.kurmann@frb.gov.
NSS_A_269360 1105..1113

1Department of Physical Therapy, Facottur Faculty, Olinda, Pernambuco, Brazil; 2Department of Physical Therapy Federal University of Pernambuco, Recife, Pernambuco, Brazil; 3Department of Rehabilitation, Hospital Otávio de Freitas, Recife, Pernambuco, Brazil; 4Sleep and Heart Laboratory, Pronto Socorro Cardiológico de Pernambuco (PROCAPE)University of Pernambuco, Recife, Pernambuco, Brazil; 5Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil Purpose: Exercise programs have been considered as an adjuvant treatment in obstructive sleep apnea (OSA). However, few studies have focused on the effects of the inspiratory muscle training (IMT) in reducing the severity and the symptoms of OSA. Patients and Methods: A randomized controlled trial was conducted and approved by the local Ethics Committee. All subjects signed the informed consent form and were randomized into 2 groups: a) IMT group (n = 8), 8 weeks of IMT with 75% of maximal inspiratory pressure (MIP) and b) placebo group (n = 8): subjects performed IMT without load. Results: IMT group showed reduction in the apnea–hypopnea index (AHI) (p = 0.01), in the Berlin questionnaire score (p = 0.001) and an increase in inspiratory muscle strength (p = 0.018). IMT group demonstrated a reduction in the AHI (31.7 ± 15.9 events/h vs 29.9 ± 15.8 events/h; p <0.001), in the Berlin questionnaire scores (2.6 ± 0.5 vs 1.2 ± 0.5; p = 0.016), Pittsburgh Sleep Quality Index (PSQI) score (7.2 ± 3.6 vs 3.7 ± 1.3; p = 0.008), in the Epworth Sleepiness Scale (ESS) (12.5 ± 4.0 vs 7.7 ± 3.0; p = 0.008) and increase in MIP (83.6 ± 26.5 cmH2O and 127.9 ± 32.5 cmH2O; p = 0.010). Conclusion: The IMT promotes discrete changes in the AHI and improves sleep quality and excessive daytime sleepiness in OSA. Moreover, IMT is a cheap, useful and simple homebased training program and can be considered as an adjunct therapy for OSA patients.
Advances and challenges in FRP strengthening of concrete structures-research at Technical University of Lodz

The paper presents state-of-the-art in the field of fiber reinforced polymer (FRP) strengthening concrete structures based on the research carried out at the Technical University of Lodz (TUL) over a last decade. The paper points out the main achievements found in the FRP strengthening for flexure, shear and confinement using both externally bonded (EB) and near surface mounted (NSM) techniques. Challenge of the FRP strengthening of concrete structures is supposed the combined NSM technique with FRP prestressing that sufficiently may improve serviceability and capacity of strengthened structures.
SCIENCE AND CULTIVATION OF EDIBLE AND MEDICINAL FUNGI

Lignosus rhinocerotis, commonly known as Tiger Milk Mushroom, has been used for more than 400 years by South East Asian communities for its healing properties on more than 15 types of medical ailments including asthma, fever, cough, cold, sinusitis, cancer, food poisoning, joint pain, and wound healing. It has also been consumed as general health tonic for improving vitality. However, research and development of this rare mushroom was hindered by its limited supply from the wild. Recent successful commercial cultivation and mass production of L. rhinocerotis TM02 has enabled numerous scientific studies to be conducted to verify claims of its bioactivity and, the efficacy and safety profile of L . rhinocerotis TM02. Scientific investigations revealed that L. rhinocerotis TM02 contains various active components such as the polysaccharides-protein complexes, β-glucan, protein, immune-modulatory proteins, all of which are biologically active in rendering significant medicinal properties such as anti-inflammatory, immuno-modulatory, anti-proliferative, antioxidative, neurite outgrowth stimulation etc. Genomic study has shown the presence of a novel fungal immuno-modulatory protein (FIP) in this mushroom. The gene sequence and three dimensional model of this novel fungal immune-modulatory protein have been recently elucidated. Pre-clinical toxicology evaluations showed that L.rhinocerotis TM02 did not induce any adverse effect on fertility, teratogenicity and genotoxicity. The no-observedadverse-effect level (NOAEL) dose of the L.rhinocerotis TM02 in a 180-day chronic toxicity study is more than 1000 mg/kg which is equivalent to 162 mg/kg human dose whilst the anticipated dose for a 60 kg adult is only ~10g. The effective dosage of L.rhinocerotis TM02 is 0.5g per day for an adult, therefore there is a wide safety margin for consumption. Based on users’ experiences and feedback, L.rhinocerotis TM02 has been shown to be effective in a broad spectrum of respiratory health and immune health conditions, maintaining the health of joints, improving vitality and stamina, and improving the quality of life of cancer patients.
Arsenic adsorptive media technology selection strategies

More stringent arsenic regulations are requiring water utilities of all sizes to install treatment systems on wells which historically only had chlorination. A case study is presented for evaluating arsenic adsorption media at a well site in Mesa, Arizona, but the approach and methodological conclusions are transferable to any arsenic treatment system. The goal of this project is to develop a comprehensive, cost-effective and rapid approach for selecting arsenic adsorptive media. Batch laboratory tests, labscale continuous flow column tests and pilot-scale tests were conducted to evaluate removal efficiency, robustness, operational benefits and cost effectiveness of eight different commercially available adsorbents. Batch tests conducted at multiple pH levels provided limited benefit when compared against continuous flow rapid small-scale column tests (RSSCTs) to evaluate arsenic removal capability by different media. Breakthrough curves of arsenic, vanadium, silica and other trace metals corresponded well between lab-scale RSSCT and field-based pilot scale, suggesting that RSSCTs offer a timesaving approach for adsorptive media selection. Leaching tests on spent media verified that media could be safely disposed and high-resolution microscopic analysis indicated that calcium and silica accumulate on media surfaces and affect the longevity of arsenic removal systems.
Topological Organization Alterations of Whole-Brain Functional Networks in Patients with Childhood Absence Epilepsy: Associations with Treatment Effects

Purpose The purpose of the current study is to detect changes of topological organization of whole-brain functional networks and their relationship with the clinical treatment effects of antiepileptic drugs (AEDs) for patients with childhood absence epilepsy (CAE) using resting-state functional MRI (RS-fMRI). Patients and Methods. RS-fMRI data from 30 CAE patients were collected and compared with findings from 30 age- and gender-matched healthy controls (HCs). The patients were treated with first-line AEDs for 46.03 months before undergoing a second RS-fMRI scan. Results CAE children at baseline showed a reduced clustering coefficient (Cp) and local efficiency (El) than the HC group, implying the reduction of functional segregation. CAE children at baseline also showed smaller characteristic path length (Lp) and higher global efficiency (Eg) compared with the HC group, implying the impairment of functional segregation. However, those metrics showed no significant differences between CAE children at follow-up and the HC group which indicated a clear renormalization of topological organization after AED treatments. CAE at follow-up also showed significantly decreased connectivity between several network regions, with which the thalamus is mainly involved. Furthermore, the reduced connectivity change between the left superior parietal gyrus and the left thalamus is positively correlated with the symptom improvements after AED treatment. Conclusion We highlighted the convergence and divergence of brain functional network dysfunctions in CAE patients and provided crucial insights into pathophysiological mechanisms and the AED effects.
Numerical investigations of the effect of strain softening on the behaviour of 1 embedded mooring chains 2 3 4

69 Mooring systems typically consist of an anchor and a mooring line and chain that connect the 70 anchor to the floating infrastructure. When the anchor connection point (the ‘padeye’) is below 71 the seabed surface, the interaction between the chain and the seabed will affect the amount of 72 load transferred to the anchor and the load angle at the padeye. Reliable methods are needed 73 therefore to assess these aspects in order to determine appropriate anchor design. 74 Available solutions for the interaction between soil and chain generally ignore any reduction in 75 the undrained shear strength of the soil as it is remoulded under the large strains associated with 76 tensioning of the anchor chain. This is an unconservative assumption for anchor design, hence 77 providing motivation for the study presented here. The system behaviour and the interaction of 78 short chain segments with the seabed have been studied using a coupled Eulerian-Lagrangian 79 (CEL) approach. The findings have led to two new design approaches that encapsulate how 80 remoulding of the soil (which affects sliding resistance more than bearing resistance) affects 81 the chain system response. Calculations using these methods captured the modelled chain 82 system response well. Both the global chain analyses and the proposed design approaches 83 suggest that approximately the entire chain load at the seabed surface (the ‘mudline’) is likely 84 to be transferred to the anchor padeye, challenging conventional design practice. 85
CFD Study on Aerodynamic Effects of NACA 2412 Airfoil as Rear Wing on a Sports Car

In the present research, aerodynamic effects of NACA 2412 airfoil as rear wing on a sports car has been investigated using Computational Fluid Dynamics (CFD) approach. The sports car has been modeled in the commercial software SOLIDWORKS 2016. Two different types of simulations were run: one for the flow around a simplified high speed sports car model with a rear wing, which is actually a NACA 2412 airfoil and the other for the flow without using a rear wing. The analysis has been carried out in ANSYS 15.0 FLUENT using k-epsilon model and for the velocity condition of 60 m/s. The effect of rear wing has been visualized from the aerodynamics perspective. Graphs of drag and lift coefficients and figures of velocity streamline, pressure distribution, Turbulence Kinetic Energy for both models have been discussed and compared. The details of the aerodynamic study has been presented in the paper.
RESPIRATORY FUNCTION IN HEALTHY FIRST-DEGREE RELATIVES OF ASTHMATIC ADOLESCENTS

It is almost a constant in the ecophysiology papers that, explicitly or implicitly, the genetic structures of the studied material were clearly present as sources of tolerance (germplasm), contrasting materials (genotypes) or simply as cultivars. The “genetic structure” of a population refers to the type, amount and distribution of genetic variation present in the population and it is expressed in terms of gene or genotype frequencies. There are five types of cultivars (populations, synthetic, pure lines, hybrids and clones) considering that all commercial varieties fit into any of these five types or their variants. The objective of this work was to analyze the ecophysiological characters studied in the major crops and their scope and limitations in relation to the genetic structure of populations. An ecophysiologist who studies physiological characters or interactions of the crop with environmental factors, should bear in mind these genetic structures when choosing materials (morphological types, cultivars or genotypes) and when he/she reaches his/her conclusions and makes recommendations, since they will determine the scope of the study. Likewise, the contributions of ecophysiology to describe classify and explain how plants perform in a particular situation and with a given genetic structure are analyzed.
Bioassays for Testing the Efficacy of Ground Sprayed Insecticides for Gypsy Moth Eradication Programs in California

The insecticides Bacillus thuringlensis (BT), carbaryl, and diflubenzuron were tested in a field-laboratory study for the effect of killing first through third instar gypsy moth larvae on foliage of valley oak trees in California. Larval mortality differed significantly between the BT, the other insecticides and the check treatments. Also, the effects of all three of the insecticides were still apparent 28 days posttreatment.
Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses

The indole-3-pyruvic acid pathway is the main route for auxin biosynthesis in higher plants. Tryptophan aminotransferases (TAA1/TAR) and members of the YUCCA family of flavin-containing monooxygenases catalyze the conversion of l-tryptophan via indole-3-pyruvic acid to indole-3-acetic acid (IAA). It has been described that jasmonic acid (JA) locally produced in response to mechanical wounding triggers the de novo formation of IAA through the induction of two YUCCA genes, YUC8 and YUC9. Here, we report the direct involvement of a small number of basic helix-loop-helix transcription factors of the MYC family in this process. We show that the JA-mediated regulation of the expression of the YUC8 and YUC9 genes depends on the abundance of MYC2, MYC3, and MYC4. In support of this observation, seedlings of myc knockout mutants displayed a strongly reduced response to JA-mediated IAA formation. Furthermore, transactivation assays provided experimental evidence for the binding of MYC transcription factors to a particular tandem G-box motif abundant in the promoter regions of YUC8 and YUC9, but not in the promoters of the other YUCCA isogenes. Moreover, we demonstrate that plants that constitutively overexpress YUC8 and YUC9 show less damage after spider mite infestation, thereby underlining the role of auxin in plant responses to biotic stress signals.
The nowcasting system INCA-BE in Belgium and its performance in different synoptic situations

Until recently, the Royal Meteorological Institute of Belgium (RMI) could not fully satisfy this demand, since it had no dedicated nowcasting system producing these nowcasts. In the beginning of 2010, the RMI started the implementation of the INCA system in Belgium: INCA-BE. INCA (Integrated Nowcasting through Comprehensive Analysis, see Haiden et al. 2011 for a comprehensive description) is a nowcasting system that has been developed at the meteorological service of Austria (ZAMG). We adapted the system for a domain around Belgium and for our local observational data flow. It was integrated in our operational chain during spring 2012. This first operational version is used internally by our forecasters, and also by some external test-users like the regional hydrological services. The system is, however, under constant development, and new features and improvements will be added in the future.