Miracle-dz/UniFAR-FTUs huggingface dataset:
Production of F4 Fimbrial Adhesin in Plants : a Model for Oral Porcine Vaccine against Enterotoxigenic Escherichia coli

10/2005 Katja Pihlainen Liquid Chromatography and Atmospheric Pressure Ionisation Mass Spectrometry in Analysing Drug Seizures 11/2005 Pietri Puustinen Posttranslational Modifications of Potato Virus A Movement Related Proteins CP and VPg 12/2005 Irmgard Suominen Paenibacillus and Bacillus Related to Paper and Spruce Tree 13/2005 Heidi Hyytiainen Regulatory Networks Controlling Virulence in the Plant Pathogen Erwinia Carotovora Ssp. Carotovora 14/2005 Sanna Janhunen Different Responses of the Nigrostriatal and Mesolimbic Dopaminergic Pathways to Nicotinic Receptor Agonists 15/2005 Denis Kainov Packaging Motors of Cystoviruses 16/2005 Ivan Pavlov Heparin-Binding Growth-Associated Molecule (HB-GAM) in Activity-Dependent Neuronal Plasticity in Hippocampus 17/2005 Laura Seppa Regulation of Heat Shock Response in Yeast and Mammalian Cells 18/2005 Veli-Pekka Jaakola Functional and Structural Studies on Heptahelical Membrane Proteins 19/2005 Anssi Rantakari Characterisation of the Type Three Secretion System in Erwinia carotovora 20/2005 Sari Airaksinen Role of Excipients in Moisture Sorption and Physical Stability of Solid Pharmaceutical Formulations 21/2005 Tiina Hilden Affinity and Avidity of the LFA-1 Integrin is Regulated by Phosphorylation 22/2005 Ari Pekka Mahonen Cytokinins Regulate Vascular Morphogenesis in the Arabidopsis thaliana Root 23/2005 Matias Palva Interactions Among Neuronal Oscillations in the Developing and Adult Brain 24/2005 Juha T. Huiskonen Structure and Assembly of Membrane-Containing dsDNA Bacteriophages 25/2005 Michael Stefanidakis Cell-Surface Association between Progelatinases and s2 Integrins: Role of the Complexes in Leukocyte Migration 26/2005 Heli Kansanaho Implementation of the Principles of Patient Counselling into Practice in Finnish Community Pharmacies 1/2006 Julia Perttila Expression, Enzymatic Activities and Subcellular Localization of Hepatitis E Virus and Semliki Forest Virus Replicase Proteins 2/2006 Tero Wennberg Computer-Assisted Separation and Primary Screening of Bioactive Compounds 3/2006 Katri Makelainen Lost in Translation: Translation Mechanisms in Production of Cocksfoot Mottle Virus Proteins 4/2006 Kari Kreander A Study on Bacteria-Targeted Screening and in vitro Safety Assessment of Natural Products 5/2006 Gudrun Wahlstrom From Actin Monomers to Bundles: The Role of Twinfilin and a-Actinin in Drosophila melanogaster Development
Secretome characterization of the lignocellulose-degrading fungi Pycnoporus sanguineus and Ganoderma resinaceum growing on Panicum prionitis biomass

ABSTRACT C4 grasses are common species in rangelands around the world and represent an attractive option for second-generation biofuel production. Although they display high polysaccharide content and reach great levels of biomass accumulation, there is a major technical issue to be addressed before they can be used for bioethanol industrial production: lignin removal. Concerning this, Pycnoporus and Ganoderma fungal genera have been highlighted due to their ability to hydrolyze lignocellulose in biological pretreatments. Our goals here were to evaluate the pretreatment efficiency using the secretome of species from Pycnoporus and Ganoderma spp. harvested from a glucose-free inductive medium (using a C4 grass) and to identify the fungal enzymatic activities responsible for the lignin degradation and glucose release. Our results show that P. sanguineus secretome exhibits a higher activity of lignocellulolytic enzymes such as cellulases, xylanases, laccases, and manganese peroxidases compared with that from G. resinaceum. Interestingly, zymograms in the presence of 2 M glucose suggest that a β-glucosidase isoform from P. sanguineus could be glucose tolerant. The proteomic approach carried out allowed the identification of 73 and 180 different proteins in G. resinaceum and P. sanguineus secretomes, respectively, which were functionally classified in five main categories and a miscellaneous group. These results open new avenues for future experimental work that lead to a deeper comprehension and a greater application of the mechanisms underlying lignocellulosic biomass degradation.
The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading

Signaling by the Rho GTPase Rac1 is key to the regulation of cytoskeletal dynamics, cell spreading and adhesion. It is widely accepted that the inactive form of Rac1 is bound by Rho GDI, which prevents Rac1 activation and Rac1-effector interactions. In addition, GDI-bound Rac1 is protected from proteasomal degradation, in line with data showing that Rac1 ubiquitination occurs exclusively when Rac1 is activated. We set out to investigate how Rac1 activity, GDI binding and ubiquitination are linked. We introduced single amino acid mutations in Rac1 which differentially altered Rac1 activity, and compared whether the level of Rac1 activity relates to Rac1 ubiquitination and GDI binding. Results show that Rac1 ubiquitination and the active Rac1 morphology is proportionally increased with Rac1 activity. Similarly, we introduced lysine-to-arginine mutations in constitutively active Rac1 to inhibit site-specific ubiquitination and analyze this effect on Rac1 signaling output and ubiquitination. These data show that the K16R mutation inhibits GTP binding, and consequently Rac1 activation, signaling and–ubiquitination, while the K147R mutation does not block Rac1 signaling, but does inhibits its ubiquitination. In both sets of mutants, no direct correlation was observed between GDI binding and Rac1 activity or -ubiquitination. Taken together, our data show that a strong, positive correlation exists between Rac1 activity and its level of ubiquitination, but also that GDI dissociation does not predispose Rac1 to ubiquitination.
Effect of planting into a green winter cereal rye cover crop on growth and development, seedling disease and yield of corn.

Terminating winter cereal rye (Secale cereale L.) cover crops (CCs) 10 or more days before planting corn is recommended to minimize seedling disease and potential yield loss. In Iowa, cold temperatures and frequent precipitation can prevent farmers from following that recommendation and sometimes forcing them to plant corn while the rye plants are still green, referred to as planting green (PG). A field trial was established to evaluate the effect of rye termination shortly before or after corn planting on growth, seedling root disease, and yield of corn. A rye CC was terminated 17 and 3 days before planting (DBP), and 6 and 12 days after planting (DAP) corn; corn planted following no rye was included as a control. Rye biomass, C:N ratio, and N accumulation increased when terminated 6 or 12 DAP corn compared with rye terminated 17 or 3 DBP corn. Corn seedlings were taller from the PG treatments. More radicle root rot was observed when rye was terminated 3 DBP, 6 DAP, and 12 DAP corn than for the 17 DBP treatment and the no-rye control. Generally, greater Pythium Clade B populations were detected on radicles and seminal roots of corn from the PG treatments. Corn populations, ears, or barren plants were not affected by the treatments. In both years, the no-rye control had the greatest corn yield and the 12 DAP treatment had the lowest yield. Our results suggest that PG increased corn seedling root disease and contributed to reduced corn yield.
The effect of memory cue duration on performance in the directed forgetting task in healthy aging

ABSTRACT Although forgetting is usually considered a memory error, intentional forgetting can function as an adaptive mechanism. The current study examined the effect of increased processing time on directed forgetting in aging as a mechanism to compensate for age-related forgetting. Specifically, an item-method directed forgetting paradigm was used in conjunction with Remember/Know/New responding to examine the effect of cue duration (1, 3, 5 s) on directed forgetting and remembering in younger and older adults. Results indicated that increased processing time improved performance in both age groups. Critically, older adults exhibited a linear increase in directed remembering performance across all cue durations which was related to individual differences in cognitive reserve. Specifically, those older adults with the highest levels of cognitive functioning showed the greatest memory benefit in the longest cue duration condition. These findings indicate the importance of processing time in accounting for intentional memory performance in older adults.
Examining the acute effects of exercise intensity on subsequent appetite, food intake, resting energy expenditure and fat oxidation.

Energy balance is important for weight maintenance with exercise having documented physiological, behavioural, and appetite effects. Exercise is known to acutely influence appetite but evidence for an independent effect of intensity is lacking. The purpose of this dissertation was to investigate the role of exercise intensity on appetite and energy intake (EI), energy expenditure (EE), and the metabolic effects of exercise intensity per se in lean and overweight individuals and to determine whether there was influence of gender or differences between groups. Forty healthy volunteers (30 lean and 10 overweight) undertook 2 periods of exercise matched for energy cost, (i) 8 repeated 60 second bouts of cycling at 95% VO2 max; high intensity exercise (HI) and (ii) 30 minutes of continuous cycling, at a fixed cadence, at 50% VO2 max; low intensity exercise (LI) in a randomised cross-over design. Satiety to a standard meal was assessed subjectively using visual analogue scales. Ad libitum intake was measured 3-h post-breakfast and for 2 days post-exercise. EE and fat oxidation were measured every 30 mins post-exercise. The results showed that in the lean group relative to LI, HI suppressed prospective food consumption, increased EE (P=0.001), fatty acid (NEFA) utilisation (P=0.004) and fat oxidation (P<0.001), but did not affect appetite, EI, plasma glucose, insulin, GLP-1 or lipid levels post-exercise. There was a differential effect of gender on prospective food consumption and NEFA response post-exercise. HI increased EE and fat oxidation post-exercise for men. In the overweight individuals, HI did not differ from LI in terms of appetite, GLP-1, glucose, insulin, lipid or NEFA levels, with no difference in EI, EE and fat oxidation post-exercise. In conclusion, there are different consequences of exercise intensity in short-term control of energy balance depending on BMI and gender; our results support the need for longer term intervention to test these mechanisms.
From mothers to children and back: Bidirectional processes in the cross‐generational transmission of anxiety from early childhood to early adolescence

Maternal psychopathology and caregiving behavior are linked with child anxiety and these associations may be particularly salient when families face mass trauma together and members influence each other's symptomatology and resilience. Despite the well‐known mother‐to‐child effects, less research addressed the longitudinal bidirectional effects of maternal and child's anxiety symptoms on each other.
Excess membrane binding of monomeric alpha-, beta- and gamma-synuclein is invariably associated with inclusion formation and toxicity

Abstract α-Synuclein (αS) has been well-documented to play a role in human synucleinopathies such as Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). First, the lesions found in PD/DLB brains—Lewy bodies and Lewy neurites—are rich in aggregated αS. Second, genetic evidence links missense mutations and increased αS expression to familial forms of PD/DLB. Third, toxicity and cellular stress can be caused by αS under certain experimental conditions. In contrast, the homologs β-synuclein (βS) and γ-synuclein (γS) are not typically found in Lewy bodies/neurites, have not been clearly linked to brain diseases and have been largely non-toxic in experimental settings. In αS, the so-called non-amyloid-β component of plaques (NAC) domain, constituting amino acids 61–95, has been identified to be critical for aggregation in vitro. This domain is partially absent in βS and only incompletely conserved in γS, which could explain why both homologs do not cause disease. However, αS in vitro aggregation and cellular toxicity have not been firmly linked experimentally, and it has been proposed that excess αS membrane binding is sufficient to induce neurotoxicity. Indeed, recent characterizations of Lewy bodies have highlighted the accumulation of lipids and membranous organelles, raising the possibility that βS and γS could also become neurotoxic if they were more prone to membrane/lipid binding. Here, we increased βS and γS membrane affinity by strategic point mutations and demonstrate that these proteins behave like membrane-associated monomers, are cytotoxic and form round cytoplasmic inclusions that can be prevented by inhibiting stearoyl-CoA desaturase.
Neurocognition of females with substance use disorder and comorbid personality disorder: Divergence in subjective and objective cognition

Abstract At least one in four patients with substance use disorder (SUD) meet criteria for personality disorder and overlapping neurocognitive deficits may reflect shared neurobiological mechanisms. We studied neurocognition in females attending residential SUD treatment by comparing SUD with (n = 20) or without (n = 30) comorbid personality disorder. Neuropsychological testing included working memory, inhibition, shifting, verbal fluency, design fluency, psychomotor speed, immediate and delayed verbal memory, processing speed, premorbid functioning, cognitive screening, and self-reported executive function. As expected, whole-sample deficits included working memory (d = –.91), self-reported executive function (d = –.87), processing speed (d = –.40), delayed verbal memory recall (d = –.39), premorbid functioning (d = –.51), and cognitive screening performance (d = –.61). Importantly, the comorbid personality disorder group showed greater self-reported executive dysfunction (d = –.67) and poorer shifting performance (d = –.65). However, they also evidenced better working memory (d = .84), immediate (d = .95) and delayed (d = .83) verbal memory, premorbid functioning (d = .90), and cognitive screening performance (d = .77). Overall executive dysfunction deficits were concordant with those observed in previous SUD studies. Surprisingly, comorbid personality disorder was associated with a pattern indicating poorer subjective (self-report) but better objective performance on a number of tasks, apart from shifting deficits that may relate to emotion dysregulation. Subjective emotional dysfunction may influence the cognitive deficits observed in the personality disorder group.
Volumetric glutamate imaging (GluCEST) using 7T MRI can lateralize nonlesional temporal lobe epilepsy: A preliminary study

Drug‐resistant epilepsy patients show worse outcomes after resection when standard neuroimaging is nonlesional, which occurs in one‐third of patients. In prior work, we employed 2‐D glutamate imaging, Glutamate Chemical Exchange Saturation Transfer (GluCEST), to lateralize seizure onset in nonlesional temporal lobe epilepsy (TLE) based on increased ipsilateral GluCEST signal in the total hippocampus and hippocampal head. We present a significant advancement to single‐slice GluCEST imaging, allowing for three‐dimensional analysis of brain glutamate networks.
Electrospun PLGA and PLGA/gelatin scaffolds for tubularized urethral replacement: Studies in vitro and in vivo

To investigate the biocompatibility of polylactic acid-glycolic acid copolymer (PLGA) and PLGA/gelatin scaffolds and their suitability for tubular urethral replacement in a canine model. PLGA and PLGA/gelatin scaffolds was constructed by electrospinning. Microstructural differences between the scaffolds was examined by Scanning electron microscopy (SEM) followed by mechanical properties testing. Biocompatibility of the material was evaluated using SEM 4, 8, 12 and 72 h after PLGA and PLGA/gelatin scaffolds co-culture with urothelial cells. And confocal analysis was also used to showed the cell adhesive and growth at 12 h. Approximately 2 cm of the anterior urethra of twelve dogs were removed and replaced with a scaffold. After the surgery for 1 month performed urethrography and for 3 month perform hematoxylin–eosin (H&E) and Masson. The results indicated that PLGA and PLGA/gelatin scaffolds had a void microfilament structure, similar to that of normal acellular matrix tissue. And the tensile strength was decreased whereas the tensile deformation and suture retention strength was increased in PLGA/gelatin scaffolds compared to that in PLGA scaffolds Urothelial cells grew well on both scaffolds. Postoperatively, animals recovered well and urinated spontaneously. However, urethrography showed varying degrees of urethral strictures in the reconstructed urethras. H&E and Masson showed that multilayer urothelial cells were formed in both the proximal and distal segments of the reconstructed urethras but without continuity. There was a small amount of smooth muscle and blood vessels under the epithelium, but regenerative urothelial cells at the midpoint of the reconstructed segment did not continue. Lots of lymphocyte infiltration was observed under the epithelium, some collagen tissue was deposited under the neo-urethral epithelium were observed. In conclusion, PLGA and PLGA/gelatin scaffolds are not suitable for tubularized urethral replacement in the canine model.
Similar crimes, similar behaviors? Comparing lone‐actor terrorists and public mass murderers

This article adds to the growth in data‐driven analyses seeking to compare samples of violent extremists with other violent populations of interest. While lone‐actor terrorists and public mass murderers are frequently treated as distinct offender types, both engage (or attempt to engage) in largely public and highly publicized acts of violence and often use similar weapons. This article investigates the (dis)similarities between both offender types. We use a series of bivariate and multivariate statistical analyses to compare demographic, psychologic and behavioral variables across 71 lone‐actor terrorists and 115 public mass murderers. The results show little distinction in sociodemographic profiles, but significant differences in (a) the degree to which they interact with co‐ideologues (b) antecedent event behaviors and (c) the degree to which they leak information before the attack. Overall, our data inform the emerging idea that lone‐actor terrorists and public mass shooters are not distinct offender types. There is more that unites them than divides them. Although the over‐arching focus of our results are on the few variables that distinguish them, the vast majority (80%+), of the 180+ variables showed no significant difference. We discuss implications for threat assessment and management in the context of these results.
Changes in the calorie and nutrient content of purchased fast food meals after calorie menu labeling: A natural experiment

Background Calorie menu labeling is a policy that requires food establishments to post the calories on menu offerings to encourage healthy food choice. Calorie labeling has been implemented in the United States since May 2018 per the Affordable Care Act, but to the best of our knowledge, no studies have evaluated the relationship between calorie labeling and meal purchases since nationwide implementation of this policy. Our objective was to investigate the relationship between calorie labeling and the calorie and nutrient content of purchased meals after a fast food franchise began labeling in April 2017, prior to the required nationwide implementation, and after nationwide implementation of labeling in May 2018, when all large US chain restaurants were required to label their menus. Methods and findings We obtained weekly aggregated sales data from 104 restaurants that are part of a fast food franchise for 3 national chains in 3 US states: Louisiana, Mississippi, and Texas. The franchise provided all sales data from April 2015 until April 2019. The franchise labeled menus in April 2017, 1 year prior to the required nationwide implementation date of May 2018 set by the US Food and Drug Administration. We obtained nutrition information for items sold (calories, fat, carbohydrates, protein, saturated fat, sugar, dietary fiber, and sodium) from Menustat, a publicly available database with nutrition information for items offered at the top revenue-generating US restaurant chains. We used an interrupted time series to find level and trend changes in mean weekly calorie and nutrient content per transaction after franchise and nationwide labeling. The analytic sample represented 331,776,445 items purchased across 67,112,342 transactions. Franchise labeling was associated with a level change of −54 calories/transaction (95% confidence interval [CI]: −67, −42, p < 0.0001) and a subsequent 3.3 calories/transaction increase per 4-week period (95% CI: 2.5, 4.1, p < 0.0001). Nationwide implementation was associated with a level decrease of −82 calories/transaction (95% CI: −88, −76, p < 0.0001) and a subsequent −2.1 calories/transaction decrease per 4-week period (95% CI: −2.9, −1.3, p < 0.0001). At the end of the study, the model-based predicted mean calories/transaction was 4.7% lower (change = −73 calories/transaction, 95% CI: −81, −65), and nutrients/transaction ranged from 1.8% lower (saturated fat) to 7.0% lower (sugar) than what we would expect had labeling not been implemented. The main limitations were potential residual time-varying confounding and lack of individual-level transaction data. Conclusions In this study, we observed that calorie labeling was associated with small decreases in mean calorie and nutrient content of fast food meals 2 years after franchise labeling and nearly 1 year after implementation of labeling nationwide. These changes imply that calorie labeling was associated with small improvements in purchased meal quality in US chain restaurants.
Hippocampal Sequencing Mechanisms Are Disrupted in a Maternal Immune Activation Model of Schizophrenia Risk

Episodic memory requires information to be stored and recalled in sequential order, and these processes are disrupted in schizophrenia. Hippocampal phase precession and theta sequences are thought to provide a biological mechanism for sequential ordering of experience at timescales suitable for plasticity. These phenomena have not previously been examined in any models of schizophrenia risk. Here, we examine these phenomena in a maternal immune activation (MIA) rodent model. We show that while individual pyramidal cells in the CA1 region continue to precess normally in MIA animals, the starting phase of precession as an animal enters a new place field is considerably more variable in MIA animals than in controls. A critical consequence of this change is a disorganization of the ordered representation of experience via theta sequences. These results provide the first evidence of a biological-level mechanism that, if it occurs in schizophrenia, may explain aspects of disorganized sequential processing that contribute to the cognitive symptoms of the disorder. SIGNIFICANCE STATEMENT Hippocampal phase precession and theta sequences have been proposed as biophysical mechanisms by which the sequential structure of cognition might be ordered. Disturbances of sequential processing have frequently been observed in schizophrenia. Here, we show for the first time that phase precession and theta sequences are disrupted in a maternal immune activation (MIA) model of schizophrenia risk. This is a result of greater variability in the starting phase of precession, indicating that the mechanisms that coordinate precession at the assembly level are disrupted. We propose that this disturbance in phase precession underlies some of the disorganized cognitive symptoms that occur in schizophrenia. These findings could have important preclinical significance for the identification and treatment of schizophrenia risk factors.