Miracle-dz/UniFAR-FTUs huggingface dataset:
New evidence on fossil eggs of Cretaceous Tyrannosaurs in eastern China

The giant fossil eggs,Macroelongatoolithus xixiaensis,laid by huge tyrannosaurs were recently discovered from Late Cretaceous Chichengshan Formation(approximately 92Ma) in Tiantai County of Zhejiang Province,though some of their fossil skull or skeleton has not yet been found in eastern China so far.Based on the taxonomy of tyrannosaurs at present,the taxonomy of their fossil egg can be proposed accordingly,i.e.Suborder: Theropoda Marsh,1881——Elongatoolithineae oosubord.nov.Superfamily: Tyrannosauroidea Osborn,1905——Macroelongatoolithoidea oosuperfam.nov.Family: Tyrannosauridae Osborn,1905——Macroelongatoolithidae oofam.nov.Elongatoolithineae oosubord.nov.Diagnosis——elliptic elongated eggs were laid in pairs and arranged abreast around a circle in a nest.Horizon——The Triassic to Cretaceous Macroelongatoolithoidea oosuperfam.nov.Diagnosis——giant flat and elliptic elongated eggs were laid in pairs and arranged abreast around a circle in a nest.Horizon——Cretaceous Macroelongatoolithidae oofam.nov.Diagnosis and Horizon——As for oosuperfamily.Oogenus Macroelongatoolithus Li et al.,1995 Type oospecies Macroelongatoolithus xixiaensis Li et al.,1995 Synonymy.2000 Macroelongatoolithus zhangi Fang et al.Which is the tyrannosaur laid the giant Macroelongatoolithus xixiaensis? Of excavated fossil tyrannosaurs from eastern Asia,an adult Guanlong and an adult Dilong were apparently too small,only 3m and 1.6m long respectively,to lay giant eggs,and it was of old age,approximately 160Ma and 130Ma respectively.An adult Alectrosaurus and an adult Alioramus were 5 m~6m long respectively,and perhaps not huge enough to lay such enormous eggs.No other than a Tarbosaurus,which an adult was more than 10 m long,was an imaginabale suspect laid these giant eggs.However all known Tarbosaurus were from terminal Cretaceous Maastrichtian(71Ma~65Ma),and were not identical with Macroelongatoolithus from early Late Cretaceous Turonian(approximately 92Ma). It is possible that the period of Tarbosaurus perhaps was extended to 92Ma,or other unknown huge tyrannosaurs laid these giant eggs in 92Ma.The binate Macroelongatoolithus xixiaensis presented in a nest indicated that the adult female tyrannosaur produced two eggs once in her nest as other theropod,i.e.they had a pair of functional oviducts.All of Macroelongatoolithus xixiaensis,furthermore,were flat oval and suggested that their eggshell were flexible as that of some modern reptiles.These eggs would be transmogrified by gravity,especially giant eggs as Macroelongatoolithus xixiaensis.
Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

This study investigated the effects of Brazilian Red Propolis (BRP) extract on seven-day-old multispecies subgingival biofilms. Mixed biofilm cultures containing 31 species associated with periodontal health or disease were grown for six days on a Calgary device. Then, mature biofilms were treated for 24 h with BRP extract at different concentrations (200–1600 µg/mL), amoxicillin (AMOXI) at 54 µg/mL (positive control) or vehicle (negative control). Biofilm metabolic activity was determined by colorimetry, and bacterial counts/proportions were determined by DNA–DNA hybridization. Data were analyzed by Kruskal–Wallis and Dunn’s tests. Treatment with BRP at 1600, 800 and 400 μg/mL reduced biofilm metabolic activity by 56%, 56% and 57%, respectively, as compared to 65% reduction obtained with AMOXI. Mean total cell counts were significantly reduced in all test groups (~50–55%). Lower proportions of red, green and yellow complex species were observed upon treatment with BRP (400 µg/mL) and AMOXI, but only AMOXI reduced the proportions of Actinomyces species. In conclusion, BRP extract was as effective as AMOXI in killing seven-day-old multispecies biofilm pathogens and did not affect the levels of the host-compatible Actinomyces species. These data suggest that BRP may be an alternative to AMOXI as an adjunct in periodontal therapy. In vivo studies are needed to validate these results.
Itaconic Acid Increases the Efficacy of Tobramycin against Pseudomonas aeruginosa Biofilms

The search for novel therapeutics against pulmonary infections, in particular Pseudomonas aeruginosa (PA) biofilm infections, has been intense to deal with the emergent rise of antimicrobial resistance. Despite the numerous achievements in drug discovery and delivery strategies, only a limited number of therapeutics reach the clinic. To allow a timely preclinical development, a formulation should be highly effective, safe, and most importantly facile to produce. Thus, a simple combination of known actives that enhances the therapeutic efficacy would be a preferential choice compared to advanced drug delivery systems. In this study, we propose a novel combination of an anti-inflammatory agent—itaconic acid (itaconate, IA)—and an approved antibiotic—tobramycin (Tob) or ciprofloxacin (Cipro). The combination of Tob and IA at a molar ratio of 1:5 increased the biofilm eradicating efficacy in the strain PA14 wild type (wt) by ~4-fold compared to Tob alone. In contrast, such effect was not observed for the combination of IA with Cipro. Subsequent studies on the influence of IA on bacterial growth, pyocyanin production, and Tob biofilm penetration indicated that complexation with IA enhanced the transport of Tob through the biofilm. We recommend the simple and effective combination of Tob:IA for further testing in advanced preclinical models of PA biofilm infections.
An X‐ray powder diffraction study of the microstructural evolution on heating 3:2 and 2:1 mullite single‐phase gels

Single‐phase gels with compositions 3Al2O3·2SiO2 and 2Al2O3·SiO2 were prepared by gelling mixtures of aluminium nitrate and tetraethylorthosilicate. Gels were fast heated at different temperatures between 900°C and 1600°C. The phase transformation and microstructural changes of both mullite precursor gels over the temperature range were followed by X‐ray powder diffraction (XRD), lattice parameter determination (LP), and scanning and transmission electron microscopies (SEM and TEM). The distribution of crystallite sizes and strains were determined by linewidth refinements of X‐ray diffraction patterns using the integral breadth method of Langford and the Warren‐Averbach analysis. XRD of both heated gels showed the formation of crystalline mullite single phase. Some amount of glassy phase coexisted with mullites at low temperatures, i. e. below 900°C. The compositional range of mullites formed on heating gels at temperatures between 900°C and 1600°C was dependent on the starting nominal composition of gels. SEM and TEM micrographs of both heated gels below 1200°C showed the formation of small, discrete, prismatic, well‐shaped nanocrystals in a very ordered arrangement. The size of these nanocrystals was dependant on the nominal composition of gels and increased on rising the heating temperature of gel precursors. The microstructural features obtained from linewidth refinement results of X‐ray diffraction patterns also allowed to suggest the formation of prismatic a little elongated nanocrystals at temperatures below 1200°C. Microstrain values were small and only displayed a relatively significant value for mullites processed at 900°C. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Evolution of starvation resistance in an invasive insect species, Aethina tumida (Coleoptera: Nitidulidae)

Abstract Starvation resistance, or the ability to survive periods without food, can shed light on selection pressure imposed by food scarcity, including chances to invade new regions as a result of human transport. Surprisingly, little information is known about starvation resistance for invasive insect species. Given that native and invasive populations differ in starvation resistance, this would suggest different selection scenarios and adaptive shifts fostering invasion success. Here, we show striking differences in starvation resistance of adult small hive beetles Aethina tumida (SHB) between native and invasive populations. In the laboratory, starvation resistance of freshly emerged laboratory‐reared and field‐collected adult females and males was evaluated in the beetle's native African range and in their invasive North American range. SHB in their native African range survived longer than SHB in their invasive North American range. Across ranges, females survived longer than males. Field‐collected SHB survived in Africa longer than freshly emerged ones, but not in the invasive range. This suggests no selection for starvation resistance in the invasive range, possibly due to differences between African and European‐derived honey bee hosts facilitating a trade‐off scenario between reproduction and starvation resistance. The ability of adult females to survive up to two months without food appears to be one factor contributing to the invasion success of this species. Assuming food availability is usually high in the invasive ranges, and trade‐offs between starvation resistance and fecundity/reproduction are common, it seems as if selection for starvation resistance during transport could set up potential trade‐offs that enhance reproduction after invasion. It would be interesting to see if this is a possible general pattern for invasive insect species.
Robust age estimation of southern sea otters from multiple morphometrics

Abstract Reliable age estimation is an essential tool to assess the status of wildlife populations and inform successful management. Aging methods, however, are often limited by too few data, skewed demographic representation, and by single or uncertain morphometric relationships. In this study, we synthesize age estimates in southern sea otters Enhydra lutris nereis from 761 individuals across 34 years of study, using multiple noninvasive techniques and capturing all life stages from 0 to 17 years of age. From wild, stranded, and captive individuals, we describe tooth eruptions, tooth wear, body length, nose scarring, and pelage coloration across ontogeny and fit sex‐based growth functions to the data. Dental eruption schedules provided reliable and identifiable metrics spanning 0.3–9 months. Tooth wear was the most reliable predictor of age of individuals aged 1–15 years, which when combined with total length, explained >93% of observed age. Beyond age estimation, dental attrition also indicated the maximum lifespan of adult teeth is 13‒17 years, corresponding with previous estimates of life expectancy. Von Bertalanffy growth function model simulations of length at age gave consistent estimates of asymptotic lengths (male Loo = 126.0‒126.8 cm, female Loo = 115.3‒115.7 cm), biologically realistic gestation periods (t 0 = 115 days, SD = 10.2), and somatic growth (male k = 1.8, SD = 0.1; female k = 2.1, SD = 0.1). Though exploratory, we describe how field radiographic imaging of epiphyseal plate development or fusions may improve aging of immature sea otters. Together, our results highlight the value of integrating information from multiple and diverse datasets to help resolve conservation problems.
Cellular Structural Studies on Jaoa prasina and Ultrastructural Studies on J.bullata

Jaoaceae is an endemic group in China.The thallus of Jaoa is disc-shaped and with more or less uneven surface.The algae always live on the rocks in clean mountain streams.This family includes one genus——Jaoa,and two species: J.prasina and J.bullata. The criterion of dividing two species is the number of cell layers.J.prasina has two layers cells,while J.bullata has three layers cells in vertical-section.This paper reported the cellular structure of the genus collected from different regions in China under the microscopy,and measured the diameter of cells in each layer,and then described the protuberance structure on the cell of surface layer.Furthermore,we observed the ultrastructure of J.bullata collected from Xiuwu County,Henan Province.The feature of mitochondria with longitudinal arrangement tubular cristae of the alga is the warrant consideration.
Targeting Mutant KRAS in Pancreatic Cancer: Futile or Promising?

Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal cancers with a dismal prognosis for the patient. This is due to limited diagnostic options for the early detection of the disease as well as its rather aggressive nature. Despite major advances in oncologic research in general, the treatment options in the clinic for PDAC have only undergone minor changes in the last decades. One major treatment advance would be the successful targeting of the oncogenic driver KRASmut. In the past, the indirect targeting of KRAS has been exploited, e. g. , via upstream inhibition of receptor tyrosine kinases or via downstream MEK or PI3K inhibition. However, the experience gained from clinical trials and from the clinic itself in the treatment of KRASmut cancer entities has dampened the initial euphoria. Lately, with the development of KRASG12C-specific inhibitors, not only the direct but also the indirect targeting of KRASmut has gained momentum again. Though preclinical studies and preliminary early clinical studies of monotherapies have shown promising results, they have been overshadowed by the swift development of resistances resulting in inconsistent responses in patient cohorts. Currently, several different combination therapies for KRASmut cancer are being explored. If they hold the promise they have made in preclinical studies, they might also be suitable treatment options for patients suffering from PDAC.
IKBKB rs2272736 is Associated with Gastric Cancer Survival

Background IKBKB/IKKβ, as the core catalytic subunit of IκB kinase complex, participates in mediation of the classical NF-κB pathway, which has been linked to inflammation and tumorigenesis. Previous studies have shown that single nucleotide polymorphisms in IKBKB have been related to gastric cancer, but how they associate to the clinical outcome is not yet clear. In this study, we retrospectively investigated the associations between single nucleotide polymorphisms located in IKBKB and gastric cancer survival. Materials and Methods IKBKB rs2272736 was genotyped in 1210 patients with primary gastric cancer in a Han Chinese population, and the relationships between rs2272736 and overall survival were evaluated. We conducted Cox proportional hazards regression, which was performed to estimate the effects of single nucleotide polymorphisms on the overall survival of patients, adjusted for potential confounding variables. Results We found that patients with rs2272736 A allele in IKBKB had significantly prolonged overall survival time compared to those with the G allele (HR = 0.83, 95% CI = 0.68–1.00, P = 0.050). In addition, AA genotype was demonstrated to have reduced risk of death for gastric cancer compared with that associated with the GG/GA genotypes, which was more common in patients with cardiac carcinoma, well-differentiated and moderately differentiated tumors, TNM Ⅰ/Ⅱ stages and intestinal type. Conclusion Our findings have shown that single nucleotide polymorphism rs2272736 in IKBKB may be a promising prognostic biomarker which should promote personalized treatment.
Interactive Effects of Glycine Equivalent, Cysteine, and Choline on Growth Performance, Nitrogen Excretion Characteristics, and Plasma Metabolites of Broiler Chickens Using Neural Networks Optimized with Genetic Algorithms

Simple Summary The negative effects of nitrogen emissions caused by animal husbandry on the environment can be reduced by lowering the crude protein content in the diets of broiler chickens. The nonessential amino acids glycine and serine, investigated together as glycine equivalent, can limit the growth of broiler chickens fed diets that are low in crude protein. The response of broiler chickens to dietary glycine equivalent is not constant and is affected by endogenous precursors of glycine equivalent and metabolic processes that dissipate glycine equivalent. Choline can be converted to glycine, and glycine equivalent is required to form cysteine from methionine. The present study investigated interactive effects among dietary glycine equivalent, cysteine, and choline in broiler chickens. The results showed that the gain:feed ratio increased with dietary glycine equivalent supplementation. The extent of interactive effects among glycine equivalent, cysteine, and choline on the gain:feed ratio was hardly pronounced. Very high nitrogen-utilization efficiency with low variation among treatments was found. The findings indicate that small differences in nitrogen-utilization efficiency caused low glycine equivalent dissipation for nitrogen excretion, likely resulting in small interactive effects among dietary glycine equivalent, cysteine, and choline. These results contribute to further dietary crude protein reduction in feed for broiler chickens. Abstract Responses of broiler chickens to dietary glycine equivalent (Glyequi) are affected by dietary cysteine and choline. Hence, this study investigated interactive effects among dietary Glyequi, cysteine, and choline on the growth of broiler chickens. Male Ross 308 broiler chickens were maintained in 105 metabolism units (10 birds/unit) from days 7 to 22. Excreta were collected in 12-h intervals from days 18 to 21. Blood was sampled on day 22 (1 bird/unit). Five levels each of Glyequi (9–21 g/kg), cysteine (2–5 g/kg), and choline (0.5–1.7 g/kg) were tested under 15 diets in 7 replicates each following a fractional central composite design. Another diet was provided to five metabolism units (15 birds/unit) to measure prececal amino acid digestibility. Data were evaluated using neural networks. The gain:feed ratio (G:F) increased with digestible Glyequi intake. Differences between low and high digestible cysteine intake were low. Effects of choline intake on G:F were low. Nitrogen-utilization efficiency (NUE) was high (≥77%), with low variation among treatments. Plasma metabolites varied among treatments and indicated that metabolism of Glyequi, cysteine, and choline was influenced. These findings showed that interactive effects of dietary Glyequi, cysteine, and choline on growth were small, possibly because NUE was barely influenced.
Aerobic Exercise-Induced Changes in Cardiorespiratory Fitness in Breast Cancer Patients Receiving Chemotherapy: A Systematic Review and Meta-Analysis

While performing aerobic exercise during chemotherapy has been proven feasible and safe, the efficacy of aerobic training on cardiorespiratory fitness (CRF) in women with breast cancer undergoing chemotherapy has not yet been systematically assessed. Therefore, the objective of this work was to determine (a) the efficacy of aerobic training to improve CRF; (b) the role of aerobic training intensity (moderate or vigorous) on CRF response; (c) the effect of the aerobic training mode (continuous or interval) on changes in CRF in women with breast cancer (BC) receiving chemotherapy. A systematic review and meta-analysis were conducted as per PRISMA guidelines, and randomized controlled trials comparing usual care (UC) and aerobic training in women with BC undergoing chemotherapy were eligible. The results suggest that increases in CRF are favored by (a) aerobic training when compared to usual care; (b) vigorous-intensity aerobic exercise (64–90% of maximal oxygen uptake, VO2max) when compared to moderate-intensity aerobic exercise (46–63% of VO2max); and (c) both continuous and interval aerobic training are effective at increasing the VO2max. Aerobic training improves CRF in women with BC undergoing chemotherapy. Notably, training intensity significantly impacts the VO2max response. Where appropriate, vigorous intensity aerobic training should be considered for women with BC receiving chemotherapy.
Refining trophic dynamics through multi‐factor Bayesian mixing models: A case study of subterranean beetles

Abstract Food web dynamics are vital in shaping the functional ecology of ecosystems. However, trophic ecology is still in its infancy in groundwater ecosystems due to the cryptic nature of these environments. To unravel trophic interactions between subterranean biota, we applied an interdisciplinary Bayesian mixing model design (multi‐factor BMM) based on the integration of faunal C and N bulk tissue stable isotope data (δ13C and δ15N) with radiocarbon data (Δ14C), and prior information from metagenomic analyses. We further compared outcomes from multi‐factor BMM with a conventional isotope double proxy mixing model (SIA BMM), triple proxy (δ13C, δ15N, and Δ14C, multi‐proxy BMM), and double proxy combined with DNA prior information (SIA + DNA BMM) designs. Three species of subterranean beetles (Paroster macrosturtensis, Paroster mesosturtensis, and Paroster microsturtensis) and their main prey items Chiltoniidae amphipods (AM1: Scutachiltonia axfordi and AM2: Yilgarniella sturtensis), cyclopoids and harpacticoids from a calcrete in Western Australia were targeted. Diet estimations from stable isotope only models (SIA BMM) indicated homogeneous patterns with modest preferences for amphipods as prey items. Multi‐proxy BMM suggested increased—and species‐specific—predatory pressures on amphipods coupled with high rates of scavenging/predation on sister species. SIA + DNA BMM showed marked preferences for amphipods AM1 and AM2, and reduced interspecific scavenging/predation on Paroster species. Multi‐factorial BMM revealed the most precise estimations (lower overall SD and very marginal beetles' interspecific interactions), indicating consistent preferences for amphipods AM1 in all the beetles' diets. Incorporation of genetic priors allowed crucial refining of the feeding preferences, while integration of more expensive radiocarbon data as a third proxy (when combined with genetic data) produced more precise outcomes but close dietary reconstruction to that from SIA + DNA BMM. Further multidisciplinary modeling from other groundwater environments will help elucidate the potential behind these designs and bring light to the feeding ecology of one the most vital ecosystems worldwide.
Endogenous Alkylglycerol Functions As a Mediator of Protein Kinase C Activity and Cell Proliferation

ENDOGENOUS ALKYLGLYCEROL FUNCTIONS AS A MEDIATOR OF PROTEIN KINASE C ACTIVITY AND CELL PROLIFERATION by F. Gregory Buchanan There is an increasing interest in the role of lipids as mediators in the regulation of many cellular activities including the control of cell proliferation. Synthetic ether linked glycerolipids such as 1-O-alky 1-sn-glycerol (alkylglycerol) have been shown to possess many biological properties. Alkylglycerol itself has been regarded only as a breakdown product of more complex ether linked lipids, typically platelet activating factor. Recently, we observed that the level of free alkylglycerol increased over 20 fold during the culture of MDCK (Madin-Darby canine kidney) cells to a contact inhibited growth state. A loss of protein kinase C (PKC) activity has also been associated with growth arrested cells. Furthermore, we have found that treatment of MDCK cells with 1-O-dodecylrac-glycerol inhibits the TPA induced release of arachidonic acid. To explore the possibility that alkylglycerol may serve a regulatory role in the control of cell proliferation or PKC activity, we examined the ability of alkylglycerol to influence PKC activity and subcellular distribution as well as the ability of alkylglycerol to effect cell proliferation. MDCK cells grown to confluence show a loss of PKC activity associated with the membrane, as reported in fibroblasts. Preconfluent cultures of MDCK cells have a high level of PKC activity associated with the membrane. However, treatment of preconfluent cultures with alkylglycerol causes a reduction of PKC activity. A similar inhibition was observed with alkylglycerol when cells were treated with TPA, an activator of PKC. To confirm that alkylglycerol was exerting an effect directly on PKC, alkylglycerol was shown to inhibit PKC activity in vitro in a dose dependent manner. Since PKC exists as a family of closely related isozymes, we have determined the effects of growth arrest and alkylglycerol treatment on PKC a, e, and C (expressed in MDCK cells). The