Miracle-dz/UniFAR-FTUs huggingface dataset:
Research on Influencing Factors and Dimensions of Health Literacy in Different Age Groups: Before and After the COVID-19 Era in Chongqing, China

Background: Understanding the levels of health literacy among different groups is essential for better public health interventions targeting specific subgroups of the population. Additionally, this article explores the prevalence and influencing factors of the health literacy levels of different age groups during the COVID-19 epidemic. Methods: Multistage stratified cluster random sampling and the Probability Proportion to Size (PPS) method were used to select permanent residents aged 15–69 in Chongqing (54,706) for the questionnaire survey. The survey period is from July 2019 and July 2020. Single-factor analysis and logistic regression models were used to study the relationship between demographics, socioeconomic factors, other independent covariates, and health literacy. Results: The health literacy levels of residents declined with age, and there were significant differences in health literacy levels between age groups (χ2 = 3332.884, P < 0.05). As far as the factors affecting health literacy level are concerned, high education and high income are the protective factors for health literacy level for residents of all ages. For adolescents (OR = 1.383, 95% CI: 1.217–1.571), young adults (OR = 1.232, 95% CI = 1.117–1.358), and middle-aged people (OR = 1.096, 95% CI = 1.017–1.182), residence in rural areas was a protective factor. In terms of the dimensions of health literacy, in particular, elderly health literacy in 2020 in Scientific Health Concepts, Safety and First Aid, Basic Medical Care decreased significantly compared with 2019. Conclusions: For adolescents, young adults, middle-aged people, to solve the problem of urban and rural health quality gap, we should not only use the geographical division, but also consider the social population and socio-economic differences. For the elderly, the following four dimensions of health literacy need to be paid more attention than those of other age: Basic Knowledge and Concepts, Scientific Health Concepts, Safety and First Aid, and Basic Medical Care. A lack of knowledge on the prevention and treatment of chronic diseases is the main reason for the recent decline in health literacy. And the health literacy among residents in major public health emergencies is needed.
A resource for improved predictions of Trypanosoma and Leishmania protein three-dimensional structure

AlphaFold2 and RoseTTAfold represent a transformative advance for predicting protein structure. They are able to make very high-quality predictions given a high-quality alignment of the protein sequence with related proteins. These predictions are now readily available via the AlphaFold database of predicted structures and AlphaFold/RoseTTAfold Colaboratory notebooks for custom predictions. However, predictions for some species tend to be lower confidence than model organisms. This includes Trypanosoma cruzi and Leishmania infantum: important unicellular eukaryotic human parasites in an early-branching eukaryotic lineage. The cause appears to be due to poor sampling of this branch of life in the protein sequences databases used for the AlphaFold database and ColabFold. Here, by comprehensively gathering openly available protein sequence data for species from this lineage, significant improvements to AlphaFold2 protein structure prediction over the AlphaFold database and ColabFold are demonstrated. This is made available as an easy-to-use tool for the parasitology community in the form of Colaboratory notebooks for generating multiple sequence alignments and AlphaFold2 predictions of protein structure for Trypanosoma, Leishmania and related species.
Effects of enzyme-based removal of collagen and elastin constituents on the biaxial mechanical responses of porcine atrioventricular heart valve anterior leaflets.

The leaflets of the atrioventricular heart valves (AHVs) regulate the one-directional flow of blood through a subtle coordination of the extracellular matrix components of the tissue, including collagen fibers, elastin fibers, and glycosaminoglycans. Dysfunction of the AHVs, such as those caused by unfavorable microstructural remodeling, lead to valvular heart diseases and improper blood flow, which can ultimately cause heart failure. In order to better understand mechanics and remodeling of the AHV leaflets and how therapeutics can inadvertently cause adverse microstructural changes, a systematic characterization of the role of each constituent in the biomechanical properties is appropriate. Previous studies have quantified the contributions of the microstructural components to the tissue-level behavior for semilunar valve cusps, but not for AHV leaflets. In this study, for the first time, we quantify the relationships between microstructure and mechanics of the AHV leaflet using a three-step experimental procedure: (i) biaxial tension and stress relaxation testing of control (untreated) porcine AHV anterior leaflet specimens; (ii) enzyme treatment to remove a portion of either the collagen or elastin constituent; and (iii) biaxial tensile and stress relaxation testing of the constituent-removed (treated) specimens. We have observed that the removal of ∼100% elastin resulted in a ∼10% decrease in the tissue extensibility with biaxial tension and a ∼75% increase in the overall stress reduction with stress relaxation. In contrast, removal of 46% collagen content from the leaflets contributed to a ∼3% increase in tissue extensibility with biaxial tension and a ∼0% increase in overall stress decay with stress relaxation. These findings provide an insight into the microstructure-mechanics relationship of the AHVs and will be beneficial for future developments and refinements of microstructurally informed constitutive models for the simulation of diseased and surgically intervened AHV functions.
The dynamic connectome of speech control

Speech production relies on the orchestrated control of multiple brain regions. The specific, directional influences within these networks remain poorly understood. We used regression dynamic causal modelling to infer the whole-brain directed (effective) connectivity from functional magnetic resonance imaging data of 36 healthy individuals during the production of meaningful English sentences and meaningless syllables. We identified that the two dynamic connectomes have distinct architectures that are dependent on the complexity of task production. The speech was regulated by a dynamic neural network, the most influential nodes of which were centred around superior and inferior parietal areas and influenced the whole-brain network activity via long-ranging coupling with primary sensorimotor, prefrontal, temporal and insular regions. By contrast, syllable production was controlled by a more compressed, cost-efficient network structure, involving sensorimotor cortico-subcortical integration via superior parietal and cerebellar network hubs. These data demonstrate the mechanisms by which the neural network reorganizes the connectivity of its influential regions, from supporting the fundamental aspects of simple syllabic vocal motor output to multimodal information processing of speech motor output. This article is part of the theme issue ‘Vocal learning in animals and humans’.
Dysfunctions, Molecular Mechanisms, and Therapeutic Strategies of Regulatory T Cells in Rheumatoid Arthritis

Regulatory T cells (Tregs) represent a distinct subpopulation of CD4+ T lymphocytes that promote immune tolerance and maintain immune system homeostasis. The dysfunction of Tregs is tightly associated with rheumatoid arthritis (RA). Although the complex pathogenic processes of RA remain unclear, studies on Tregs in RA have achieved substantial progress not only in fundamental research but also in clinical application. This review discusses the current knowledge of the characterizations, functions, and molecular mechanisms of Tregs in the pathogenesis of RA, and potential therapies for these disorders are also involved.
Triple Oxygen Isotope Measurements (Δ'17O) of Body Water Reflect Water Intake, Metabolism, and δ18O of Ingested Water in Passerines

Understanding physiological traits and ecological conditions that influence a species reliance on metabolic water is critical to creating accurate physiological models that can assess their ability to adapt to environmental perturbations (e.g., drought) that impact water availability. However, relatively few studies have examined variation in the sources of water animals use to maintain water balance, and even fewer have focused on the role of metabolic water. A key reason is methodological limitations. Here, we applied a new method that measures the triple oxygen isotopic composition of a single blood sample to estimate the contribution of metabolic water to the body water pool of three passerine species. This approach relies on Δ'17O, defined as the residual from the tight linear correlation that naturally exists between δ17O and δ18O values. Importantly, Δ'17O is relatively insensitive to key fractionation processes, such as Rayleigh distillation in the water cycle that have hindered previous isotope-based assessments of animal water balance. We evaluated the effects of changes in metabolic rate and water intake on Δ'17O values of captive rufous-collared sparrows (Zonotrichia capensis) and two invertivorous passerine species in the genus Cinclodes from the field. As predicted, colder acclimation temperatures induced increases in metabolic rate, decreases in water intake, and increases in the contribution of metabolic water to the body water pool of Z. capensis, causing a consistent change in Δ'17O. Measurement of Δ'17O also provides an estimate of the δ18O composition of ingested pre-formed (drinking/food) water. Estimated δ18O values of drinking/food water for captive Z. capensis were ~ −11‰, which is consistent with that of tap water in Santiago, Chile. In contrast, δ18O values of drinking/food water ingested by wild-caught Cinclodes were similar to that of seawater, which is consistent with their reliance on marine resources. Our results confirm the utility of this method for quantifying the relative contribution of metabolic versus pre-formed drinking/food water to the body water pool in birds.
Spin Seebeck Effect in a Hybridized Quantum-Dot/Majorana-Nanowire With Spin Heat Accumulation

Properties of spin Seebeck effect (SSE) in a quantum dot (QD) connected to a topological superconductor or semiconductor nanowire with strong spin-orbit interaction are theoretically studied by the noneqilibrium Green’s function method combined with Dyson equation technique. At low temperatures, Majorana zero modes (MZMs) are prepared at the ends of topological superconductor or semiconductor nanowire, and are hybridized to the QD with spin-dependent strength. We consider that the QD is coupled to two leads in the presence of spin heat accumulation (SHA), i.e., spin-dependent temperature in the leads. We find that the thermopower is spin-polarized when the hybridization strength between the QD and one mode of the MZMs depends on electron spin direction, and its spin-polarization can be effectively adjusted by changing the magnitude of SHA. By proper variation of the spin-polarization of the QD-MZM hybridization strength, magnitude of the SHA, dot level, or the direct coupling between the MZMs, 100% spin-polarized or pure thermopower can be generated. Our results may find real usage in high efficiency spintronic devices or detection of the MZMs, which are under current extensive study. The present model is within the reach of current nano-technologies and may by used in high efficiency spin caloritronics devices.
Letermovir Prophylaxis for Cytomegalovirus Infection in Allogeneic Stem Cell Transplantation: A Real-World Experience

Despite effective treatments, cytomegalovirus (CMV) continues to have a significant impact on morbidity and mortality in allogeneic stem cell transplant (allo-SCT) recipients. This multicenter, retrospective, cohort study aimed to evaluate the reproducibility of the safety and efficacy of commercially available letermovir for CMV prophylaxis in a real-world setting. Endpoints were rates of clinically significant CMV infection (CSCI), defined as CMV disease or CMV viremia reactivation within day +100-+168. 204 adult CMV-seropositive allo-SCT recipients from 17 Italian centres (median age 52 years) were treated with LET 240 mg/day between day 0 and day +28. Overall, 28.9% of patients underwent a haploidentical, 32.4% a matched related, and 27.5% a matched unrelated donor (MUD) transplant. 65.7% were considered at high risk of CSCI and 65.2% had a CMV seropositive donor. Low to mild severe adverse events were observed in 40.7% of patients during treatment [gastrointestinal toxicity (36.3%) and skin rash (10.3%)]. Cumulative incidence of CSCI at day +100 and day +168 was 5.4% and 18.1%, respectively, whereas the Kaplan-Meier event rate was 5.8% (95% CI: 2.4-9.1) and 23.3% (95% CI: 16.3-29.7), respectively. Overall mortality was 6.4% at day +100 and 7.3% at day +168. This real-world experience confirms the efficacy and safety of CMV.
Ephemerality in Social Media: Unpacking the Personal and Social Characteristics of Time Limit Users on WeChat Moments

Social media platforms increasingly give users the option of ephemerality through settings that delete or hide posted content after a set period of time. Many individuals apply these settings to manage their posting history and, in turn, reduce concerns about self-presentation. Despite the growing popularity of this feature, few studies have empirically explored it. This study examines the Time Limit setting on WeChat Moments as an example and investigates how users using the Time Limit setting differ from nonusers in terms of personal characteristics (demographics, personality traits, psychological factors, and previous behavioral patterns) and social characteristics (audience size and audience diversity). Compared with nonusers, users using Time Limit setting scored significantly higher on posting frequency and privacy setting use and scored significantly lower on audience size. We also examine how personal and social characteristics vary between user groups with different degrees of ephemerality (i.e., low, medium, or high). Our findings show that users using the Time Limit setting who scored higher on measures of life changes, self-monitoring, posting frequency, and audience size and lower on perceived stress were more likely to opt for the low (i.e., 6months) rather than the medium (i.e., 1month) or high (i.e., 3days) degree of ephemerality. Our work contributes to the understanding of ephemerality settings on social media platforms and provides insights that help practitioners design more effective platforms.
Mate choice enhances post-zygotic barriers to gene flow via ancestry bundling

Hybridization and subsequent genetic introgression are now known to be common features of the histories of many species, including our own. Following hybridization, post-zygotic selection tends to purge introgressed DNA genome-wide. While mate choice can prevent hybridization in the first place, it is also known to play an important role in post-zygotic selection against hybrids, and thus the purging of introgressed DNA. However, this role is usually thought of as a direct one: a mating preference for conspecifics reduces the sexual fitness of hybrids, reducing the transmission of introgressed ancestry. Here, we explore a second, indirect role of mate choice as a barrier to gene flow. Under assortative mating, parents covary in their ancestry, causing ancestry to be “bundled” in their offspring and later generations. This bundling effect increases ancestry variance in the population, enhancing the efficiency with which post-zygotic selection purges introgressed DNA. Using whole-genome simulations, we show that the bundling effect can comprise a substantial portion of mate choice’s overall effect as a postzygotic barrier to gene flow, and that it is driven by ancestry covariances both between and within maternally and paternally inherited genomes. Using estimates of the strength of assortative mating in avian hybrid zones, we calculate that the bundling effect of mate choice may increase the amount of purging of introgressed DNA by 40-80%, contributing substantially to the genetic isolation of species.
Elucidating the neural mechanisms of 1 Learning-to-Learn

12 Learning-to-learn, a progressive acceleration of learning while solving a series 13 of similar problems, represents a core process of knowledge acquisition that draws 14 attention in both neuroscience and artificial intelligence. To investigate its under15 lying brain mechanism, we trained a recurrent neural network model on arbitrary 16 sensorimotor mappings. The network displayed an exponential speedup in learn17 ing. The neural substrate of a schema emerges within a low-dimensional subspace 18 of population activity. Its reuse in new problems facilitates learning by limiting 19 connection weight changes. Since the population trajectory of a recurrent net20 work produces behavior, learning is determined by the vector field changes. We 21 propose a novel analysis of weight-driven vector field changes, which showed that 22 novel stimuli in new problems can distort the schema representation. Weight 23 changes eliminate such distortions and improve the invariance of the reused rep24 resentations in future learning. The accumulation of such weight changes across 25 problems underlies the learning-to-learn dynamics. 26
Gender and illegal wildlife trade: overlooked and underestimated

ness. The species is not listed on the CITES appendices, although indiscriminate logging is a major problem, including a current increase in the Ebo forest, which was previously considered a safe haven for this and other species. During August –March  we surveyed the Ebo forest to collect information on the rate of harvesting of this species and on the actors involved in the trade. We estimated that c.  t ofM. bisulcata are smuggled out of the forest every month. In February  the area was gazetted as a logging concession. Given the current rate of illegal harvesting, we predict that within the next  years all seed-bearing individuals will have been logged, pushing the species to the brink of extinction. We identified five groups of actors involved in the trade of this wood: initiators (landowners, or locally influential people), prospectors (local men paid to find and mark trees), harvesters (local men paid to cut the marked trees), transporters (owners of trucks paid to transport the logged wood to the nearby city of Douala) and buyers (local businessmen or foreigners). We learnt that Chinese traders based in Douala pay USD /m of M. bisulcata wood, the price of which as doubled since . The high price is probably the main reason for the current increase in illegal commercial harvesting. In addition, however, the decision to gazette the Ebo forest as a logging concession has inadvertently encouraged local people to log valuable timber products before they are forbidden to access the forest. Based on our findings, we make the following recommendations: () the Ebo forest should be gazetted as a National Park, () the area needs to be reforested urgently with M. bisulcata, () alternative livelihood activities need to be implemented for the people living in the vicinity of the Ebo forest, and () M. bisulcata needs to be listed on a CITES appendix, prohibiting international trade in its wood. ERIC DJOMO NANA ( orcid.org/0000-0002-6118-9359), CECILIA MANDAH TAKOR, STANDLY NKEMNYI NKENGBEZA, BARTHELEMY TCHIENGUE, ERIC T. NGANSOP and EMMANUEL TCHOPWE National Herbarium of Cameroon, Yaoundé, Cameroon. E-mail eric.nana@natconbio.org
Interactions and Cytotoxicity of Human Neurodegeneration- Associated Proteins Tau and α-Synuclein in the Simple Model Dictyostelium discoideum

The abnormal accumulation of the tau protein into aggregates is a hallmark in neurodegenerative diseases collectively known as tauopathies. In normal conditions, tau binds off and on microtubules aiding in their assembly and stability dependent on the phosphorylation state of the protein. In disease-affected neurons, hyperphosphorylation leads to the accumulation of the tau protein into aggregates, mainly neurofibrillary tangles (NFT) which have been seen to colocalise with other protein aggregates in neurodegeneration. One such protein is α-synuclein, the main constituent of Lewy bodies (LB), a hallmark of Parkinson’s disease (PD). In many neurodegenerative diseases, including PD, the colocalisation of tau and α-synuclein has been observed, suggesting possible interactions between the two proteins. To explore the cytotoxicity and interactions between these two proteins, we expressed full length human tau and α-synuclein in Dictyostelium discoideum alone, and in combination. We show that tau is phosphorylated in D. discoideum and colocalises closely (within 40 nm) with tubulin throughout the cytoplasm of the cell as well as with α-synuclein at the cortex. Expressing wild type α-synuclein alone caused inhibited growth on bacterial lawns, phagocytosis and intracellular Legionella proliferation rates, but activated mitochondrial respiration and non-mitochondrial oxygen consumption. The expression of tau alone impaired multicellular morphogenesis, axenic growth and phototaxis, while enhancing intracellular Legionella proliferation. Direct respirometric assays showed that tau impairs mitochondrial ATP synthesis and increased the “proton leak,” while having no impact on respiratory complex I or II function. In most cases depending on the phenotype, the coexpression of tau and α-synuclein exacerbated (phototaxis, fruiting body morphology), or reversed (phagocytosis, growth on plates, mitochondrial respiratory function, Legionella proliferation) the defects caused by either tau or α-synuclein expressed individually. Proteomics data revealed distinct patterns of dysregulation in strains ectopically expressing tau or α-synuclein or both, but down regulation of expression of cytoskeletal proteins was apparent in all three groups and most evident in the strain expressing both proteins. These results indicate that tau and α-synuclein exhibit different but overlapping patterns of intracellular localisation, that they individually exert distinct but overlapping patterns of cytotoxic effects and that they interact, probably physically in the cell cortex as well as directly or indirectly in affecting some phenotypes. The results show the efficacy of using D. discoideum as a model to study the interaction of proteins involved in neurodegeneration.