Miracle-dz/UniFAR-FTUs huggingface dataset:
Endophytic Fungal and Bacterial Microbiota Shift in Rice and Barnyardgrass Grown under Co-Culture Condition

Although barnyardgrass (Echinochloa crus-galli L.) is more competitive than rice (Oryza sativa L.) in the aboveground part, little is known about whether barnyardgrass is still competitive in recruiting endophytes and the root microbiota composition variation of rice under the barnyardgrass stress. Here, by detailed temporal characterization of root-associated microbiomes of rice plants during co-planted barnyardgrass stress and a comparison with the microbiomes of unplanted soil, we found that the bacterial community diversity of rice was dramatically higher while the fungal community richness was significantly lower than that of barnyardgrass at BBCH 45 and 57. More importantly, rice recruited more endophytic bacteria at BBCH 45 and 57, and more endophytic fungi at BBCH 17, 24, 37 to aginst the biotic stress from barnyardgrass. Principal coordinates analysis (PCoA) showed that rice and barnyardgrass had different community compositions of endophytic bacteria and fungi in roots. The PICRUSt predictive analysis indicated that majority of metabolic pathways of bacteria were overrepresented in barnyardgrass. However, eleven pathways were significantly presented in rice. In addition, rice and barnyardgrass harbored different fungal trophic modes using FUNGuild analysis. A negative correlation between bacteria and fungi in rice and barnyardgrass roots was found via network analysis. Actinobacteria was the vital bacteria in rice, while Proteobacteria dominated in barnyardgrass, and Ascomycota was the vital fungi in each species. These findings provided data and a theoretical basis for the in-depth understanding of the competition of barnyardgrass and endophytes and have implications relevant to weed prevention and control strategies using root microbiota.
Serum Insulin-like Growth Factor-1 Is a Biomarker of Testosterone Production and Intact Acrosome in Asian Elephants (Elephas maximus)

Simple Summary In Thailand, the low fertility rate of Asian elephants has been identified. Factors contributing to poor semen quality in the elephants are not fully understood. Insulin-like growth factor-1 (IGF-1) is related to male infertility. It plays an essential role in testicular development by stimulating cell proliferation, differentiation, and steroidogenesis. In addition, there is increasing evidence that IGF-1 plays a critical role in spermatogenesis. This may be conducive to finding the causes of poor sperm quality in Asian elephants (Elephas maximus). In the present study, we investigated the relationships among serum IGF-1, serum testosterone level, and semen parameters in seven elephant bulls. The findings suggest that serum IGF-1 concentration is likely to predict sperm quality like acrosome integrity. The further mechanism by which IGF-1 affects sperm quality requires further investigation. Abstract The objective of this study was to find relationships among serum IGF-1, serum testosterone, seminal plasma IGF-1 concentrations and semen parameters in Asian elephants (Elephas maximus). A total of 17 ejaculates (one to three ejaculates/bull) were collected from seven captive elephant bulls by performing rectal massage. Before each ejaculation, blood samples were obtained for serum IGF-1 and testosterone assays. Subsequently, the semen characteristics of each ejaculate were evaluated. Mean serum IGF-1 concentration of elephant bulls was estimated as 326.3 ± 114.6 ng/mL (median, 286.2 ng/mL; range, 167.4–542.7 ng/mL). An increase in serum IGF-1 concentration was found to correlate with the percentage of spermatozoa with intact acrosomes. In addition, IGF-1 concentration was positively correlated with testosterone level. However, seminal IGF-1 concentrations could not be detected. In conclusion, our findings suggest that serum IGF-1 concentration is likely a biomarker of normal testicular functions, particularly spermatogenesis in elephants. Moreover, this commercial IGF-1 ELISA is eligible for analyzing serum IGF-1 concentration in Asian elephants.
Association of PCSK9 levels and genetic polymorphisms with stroke recurrence and functional outcome after acute ischemic stroke

Background Protein convertase subtilisin/kexin type 9 (PCSK9) is a hepatic protein that participated in the lipid homeostasis. Its high levels and polymorphisms are associated with high low-density lipoprotein cholesterol, increasing the vascular risk potentially. However, the association between PCSK9 levels, genetic polymorphisms, and ischemic stroke remains unclear. We aimed to study the relationship between PCSK9 levels, genetic polymorphisms, and stroke outcomes in patients with ischemic stroke. Methods A total of 9,782 acute ischemic stroke patients registered in the China National Stroke Registry-III were included in this prospective study. Circulating PCSK9 concentrations and 11 key single-nucleotide polymorphisms (SNPs) were examined. The clinical outcomes included stroke recurrence, death, and poor functional outcome at 12 months. Results The median PCSK9 level was 361.28 ng/mL. After adjusting for confounders, patients in the highest quartile of circulating PCSK9 had a relatively lower risk of 12-month stroke recurrence (HR 0.80, 95% CI: 0.67–0.96). No significant relationship between PCSK9 level and death or poor functional outcome was found. No significant relationship between SNPs and stroke outcomes at 12 months was found. Conclusions The high level of PCSK9 was associated with decreased stroke recurrence at 12 months in ischemic stroke patients. There was no significant association between PCSK9 polymorphisms and acute ischemic stroke based on a Chinese registry.
Preferential solvation of 5,7-dihydroxyflavone (chrysin) in aqueous co-solvent mixtures of methanol and ethanol

ABSTRACT The inverse Kirkwood-Buff integrals were used to calculate the preferential solvation parameters of the natural polyphenolic compound chrysin in binary mixtures of ethanol (1) + water (2) and methanol (1) + water (2) as a function of their thermodynamic properties. At temperatures between 298.15 K and 318.15 K, chrysin is extremely sensitive to specific solvation effects; therefore, the negative values of were found in the water-rich range of 0 < x1 < 0.30 for methanol (1) + water (2) and 0 < x1 < 0.25 for ethanol (1) + water (2) which suggested that water (2) preferentially dissolves chrysin (3). In co-solvent-rich regions, the values of were positive, and the co-solvents preferentially dissolve chrysin (3). The solvation of drugs in water-rich mixtures is likely to be facilitated by hydrophobic hydration around aromatic rings or groups.
High P-T Calcite-Aragonite Phase Transitions Under Hydrous and Anhydrous Conditions

The subduction of calcite into deep Earth and subsequent phase change are important for global carbon cycle. However, the study of the phase boundary between calcite and aragonite under high P-T conditions is insufficient due to sparse existing phase points and narrow pressure range. In addition, the impact of aqueous fluid on the phase transition requires further investigation. In this work, the calcite-aragonite phase transitions in both anhydrous and hydrous conditions were studied using diamond anvil cell (DAC) with in-situ Raman spectroscopy. In the anhydrous condition, investigations were conducted up to 12 GPa and 400°C and only the solid recrystallization-reconstructive (SRR) phase transition was observed. The calcite-aragonite boundary shows a convex upward curve with the minimum transformation temperature at around 150°C and a wide transformation pressure range from 1 to 12 GPa, consistent with the molar volume change between aragonite and calcite-I/II/III/IIIb. In the hydrous condition, both the SRR phase transition and dissolution-precipitation-dehydration (DPD) phase transition were observed under different heating conditions, and in the DPD phase transition ikaite serves as an intermediate phase precipitated from dissolved calcite and then dehydrates into aragonite. Our results suggest the phase transition of calcite-aragonite in the subduction zone, where the SRR phase transition can exist in slabs under wide P-T conditions (1–2 GPa and 160–400°C), and the DPD process can only occur under lower P-T conditions (less than 1.5 GPa and 110°C).
Efficacy of Pirfenidone vs. Placebo in Unclassifiable Interstitial Lung Disease, by Surgical Lung Biopsy Status: Data From a post-hoc Analysis

Approximately 12–13% of patients with interstitial lung disease (ILD) are diagnosed with unclassifiable ILD (uILD), often despite thorough evaluation. A recent Phase 2 study (NCT03099187) described a significant effect of pirfenidone vs. placebo on forced vital capacity (FVC) measured by site spirometry in patients with progressive fibrosing uILD (hereafter referred to as the pirfenidone in uILD study). Here, we present the results from a post-hoc analysis of this study to assess patient baseline characteristics and the efficacy of pirfenidone vs. placebo analyzed by surgical lung biopsy (SLB) status. Mean FVC (mL) change over 24 weeks was included as a post-hoc efficacy outcome. Of 253 randomized patients, 88 (34.8%) had a SLB and 165 (65.2%) did not. Baseline characteristics were generally similar between SLB subgroups; however, patients who had a SLB were slightly younger and had a higher 6-min walk distance than those without a SLB. Mean FVC change over 24 weeks for pirfenidone vs. placebo was −90.9 vs. −146.3 mL, respectively, in patients who had a SLB, and 8.2 vs. −85.3 mL, respectively, in patients without a SLB. Overall, the results from the post-hoc analysis identified that pirfenidone may be an effective treatment in progressive fibrosing uILD over 24 weeks, irrespective of SLB status; however, caution should be taken when interpreting these data due to several limitations. There are differences in the treatment effect of pirfenidone between the subgroups that require further pathological and radiological investigation. In this manuscript, we also descriptively compared baseline characteristics from the overall pirfenidone in uILD study population with other uILD populations reported in the literature, with the aim of understanding if there are any similarities or differences within these cohorts. Most baseline characteristics for patients in the pirfenidone in uILD study were within the ranges reported in the literature; however, ranges were wide, highlighting the heterogeneity of uILD populations. Clinical Trial Registration ClinicalTrials.gov, identifier: NCT03099187.
An Adolescent Patient With Idiopathic Pulmonary Arterial Hypertension Weaned Off Intravenous Epoprostenol Following Treatment With Selexipag: A Case Report

Idiopathic pulmonary arterial hypertension (PAH) is a rare, progressive disease affecting the pulmonary arteries. Epoprostenol, a synthetic prostaglandin analog, is the most potent pharmacological treatment modality used in patients with PAH. However, it requires continuous intravenous infusion, which negatively impacts the patient’s quality of life and frequently results in complications, such as catheter-related bloodstream infection. We weaned an adolescent female patient off epoprostenol by gradually introducing oral selexipag over a sustained period, following many years of continuous intravenous epoprostenol use alone. Oral selexipag might have an efficacy comparable to epoprostenol in young patients with PAH.
Design, Synthesis and Characterization of Vitrimers with Low Topology Freezing Transition Temperature

Vitrimers are crosslinked polymeric materials that behave like fluids when heated, regulated by the kinetics of internal covalent bond-exchange that occurs rapidly at or above the topology freezing transition temperature (Tv) of the vitrimer, making these materials readily reprocessable and recyclable. We report two novel multiphase vitrimeric materials prepared by the cross-linking of two polymers, namely poly(triethylene glycol sebacate) and poly(2-hydroxyethyl acrylate), using zinc acetate or tin(II) 2-ethylhexanoate as catalysts, which exhibit significantly low Tv temperatures of 39 °C and 29 °C, respectively. The transesterification reactions allow rapid and pronounced stress relaxation at high temperatures, following the Arrhenius law. The lower Tv of these vitrimers could be attributable to the flexible long chains of these polymers and the significant excess of OH moieties present along the main chain of the polymer. The design of such multiphase vitrimers is not only useful for the practical application of vitrimers to reduce plastic waste but could also facilitate further development of functional polymer materials that can be reprocessed at low temperatures.
Joint pricing and inventory strategies in a supply chain subject to inventory inaccuracy

This paper focuses on pricing strategies, inventory policies for a supply chain when Radio Frequency Identification (RFID) technology is adopted to cope with inventory inaccuracy. The supply chain consists of one supplier and one retailer, in which the RFID tag price is shared between the supplier and the retailer. We present and compare the performance differences between a wholesale price contract and a consignment contract when the retailer is the Stackelberg leader and the supplier is the follower. Based on the optimal pricing and inventory decisions, an interesting observation of contract selection is that there are two critical values of inventory available rate such that when the inventory availability is less than the lower value, both the supplier and the retailer prefer a consignment contract; when the inventory availability is greater than the upper value, a wholesale price contract is their best choice; when the inventory availability is between the two values, the supplier prefers a wholesale price contract and the retailer prefers a consignment contract. Additionally, there exist threshold values of RFID tag price and sharing rate to determine the contract preference for the retailer. Furthermore, the profits of both the supplier and the retailer are independent of the RFID tag price sharing rate in a wholesale price contract, and the supplier has the incentive to invest in RFID tag cost in a consignment contract.
Antibiotics susceptibility against Pseudomonas aeruginosa isolated from different infections

In the present study, a total 70 samples were collected from different sites of infection which include 25(35.7%) samples from bronchial wash, 15 (21.4%) samples from urine, 15 (21.4%) samples from ear swabs, and 15 (21.4%) samples wound swabs), these samples have been collected and tested during period from March 2022 to June 2022. The results showed that, out of 70 samples, 62(88.5%) give positive culture, while 8(11.5%) samples were negative culture. Out of 62 positive culture on different types of growth media, and the bacterial was identified according to gram stain, biochemical tests and Vitek compact system, the results showed that, only 18(29.1%) isolates were related to Pseudomonas aeruginosa, while 44 (70.9%) samples were related to other types of microbial agents, these isolates which include 5/18(28%) isolates from bronchial wash, 4/18(22.1%) isolates from urine, 2/18(11.1%) isolates from ear swabs and 7/18(38.8%) isolates from wound swabs. To confirm the isolates of bacteria was used automated Compact Vitek-2 system use GN-ID cards which contained 64 biochemical tests.
Few-Shot Scene Classification of Optical Remote Sensing Images Leveraging Calibrated Pretext Tasks

Small data hold big artificial intelligence (AI) potential. As one of the promising small data AI approaches, few-shot learning has the goal to learn a model efficiently that can recognize novel classes with extremely limited training samples. Therefore, it is critical to accumulate useful prior knowledge obtained from large-scale base class dataset. To realize few-shot scene classification of optical remote sensing images, we start from a baseline model that trains all base classes using a standard cross-entropy loss leveraging two auxiliary objectives to capture intrinsical characteristics across the semantic classes. Specifically, rotation prediction learns to recognize the 2-D rotation of an input to guide the learning of class-transferable knowledge, and contrastive learning aims to pull together the positive pairs while pushing apart the negative pairs to promote intraclass consistency and interclass inconsistency. We jointly optimize two such pretext tasks and semantic class prediction task in an end-to-end manner. To further overcome the overfitting issue, we introduce a regularization technique, adversarial model perturbation, to calibrate the pretext tasks so as to enhance the generalization ability. Extensive experiments on public remote sensing benchmarks including Northwestern Polytechnical University (NWPU)-RESISC45, aerial image dataset (AID), and Wuhan University (WHU)-remote sensing (RS)-19 demonstrate that our method works effectively and achieves best performance that significantly outperforms many state-of-the-art approaches.
Prevalence of Hypertension and Risk Factors associated with it in Subjects attending Health Camp in Rishikesh, Uttarakhand

Background: One of the most important causes of death in the world is hypertension and its epidemic seems to be growing. By the year 2025, around 1.5 billion adults will be living with hypertension. Aim & Objectives: To find prevalence of hypertension in subjects attending a health screening camp at SPS government hospital, Rishikesh and to identify various risk factors related to hypertension. Material & Methods: A cross-sectional study was conducted among subjects attending a health screening camp at SPS Government hospital, Rishikesh. The camp was conducted for seven days period, from 11th October- 17th October, 2017. All subjects that attended the health camp and were either 30 years old and/or above were included in this study. An informed written consent was taken from all subjects that consented to participate in this study after explaining to them the purpose of the study. A questionnaire that was predesigned, pretested, structured was used to obtain demographic data, personal data and history of hypertension in the family, behavioural aspects, including, use of tobacco, alcohol and dietary habits. Blood pressure (BP) and anthropometric measurements were also recorded in this study. Total of 207 people attended the health camp, from which 196 agreed to participate in study. Results: Out of total of 207 people that attended the camp, 196 people gave their consent to participate in the study. In this study, out of 196 subjects 66 (33.7%) were found to be hypertensive. Risk factors like increasing age, family history of hypertension, increased BMI and waist hip ratio, use of tobacco and alcohol and increased blood sugar levels were found to be associated significantly with hypertension. Conclusion: There is high prevalence of hypertension in Rishikesh. Efforts are required to regularly screen for blood pressure at the community level to help tackle this iceberg disease.
Immunogenicity of Monoclonal Antibodies and the Potential Use of HLA Haplotypes to Predict Vulnerable Patients

The use of monoclonal antibodies (mAbs) in the clinic has successfully expanded to treatment of cancer, viral infections, inflammations, and other indications. However, some of the classes of mAbs that are used in the clinic show the formation of anti-drug antibodies (ADAs) leading to loss of efficacy. This review describes ADA formation for the various mAbs, and its clinical effect. Lastly, this review considers the use of HLA-haplotypes as biomarkers to predict vulnerability of patients sensitive to formation of ADAs.
Transcriptome and Differentially Expressed Gene Profiles in Mycelium, Primordium and Fruiting Body Development in Stropharia rugosoannulata

Stropharia rugosoannulata uses straw as a growth substrate during artificial cultivation and has been widely promoted in China. However, its fruiting body formation and development processes have not been elucidated. In this study, the developmental transcriptomes were analyzed at three stages: the mycelium (G-S), primordium (P-S) and fruiting body (M-F) stages. A total of 9690 differentially expressed genes (DEGs) were identified in the different developmental stages. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these DEGs were involved mainly in hydrolase activity, structural molecule activity and oxidoreductase activity as well as xenobiotic biodegradation and metabolism and energy metabolism pathways. We further found that the higher expression of most carbohydrate enzyme (i.e., GH, CE, CBM, AA and PL) genes in the hyphal (i.e., G-S) stage was related mainly to substrate degradation, while the upregulation of glycosyltransferase (GT) gene expression in the P-S and M-F stages may be related to cell wall synthesis. In addition, we found that CO2-sensing-related genes (i.e., CA-2, CA-3, PKA-1 and PKA-2) were upregulated in the P-S and M-F stages, heat shock protein genes (HSP60 and HSP90) were significantly downregulated in the P-S stage and upregulated in the M-F stage and the transcription factors (i.e., steA, MYB, nosA, HAP1, and GATA-4/5/6) involved in growth and development were significantly upregulated in the P-S stage. These results suggest that environmental factors (i.e., CO2 and temperature) and transcription factors may play a key role in primordium formation. In short, this study provides new insights into the study of stimulating primordia formation affecting the development of fruiting bodies of S. rugosoannulata.
Association between anthropometry and cardiovascular risk in patients attending a diabetic clinic

Background Obesity is a well-documented risk factor for cardiovascular disease (CVD) in type 2 diabetes mellitus (T2DM), with increasing evidence to suggest visceral adiposity as a greater risk factor for CVD than body mass index (BMI). Objectives To determine a relationship between hypertension (HPT) and anthropometry in people living with diabetes (PLWD) in an HIV endemic area. Methods This was a retrospective study analysing data captured from standardised clinic sheets from the DM clinic at the Harry Gwala Regional Hospital, Pietermaritzburg, South Africa, from January 1, 2019 to December 31, 2019. Results Data from 957 PLWD were used for the study, the majority of whom had T2DM (811; 86.2%). Approximately one-sixth of the cohort had HIV infection (146; 15.3%). There was no significant difference in HPT prevalence between the HIV-uninfected (77.9%) and PLWD who had HIV (PLWDHIV) (78.1%). Multivariate analysis revealed females with increased waist circumference (WC) and waist-to-height ratio (WTHR) were 57.8 (95% CI 3.04–1096.33) (p = 0.007) and 87.2 (95% CI 4.88–1558.28) (p = 0.002) times more likely to be hypertensive respectively. By contrast, only BMI in males was associated with HPT with a AOR 5.294 (95% CI 1.54 - 18.22) (p = 0.008). HIV status was non-contributory to anthropometry in predicting HPT in PLWD. Conclusion Our study found that anthropometric indices are not all equal predictors of HPT. The authors advocate for local guidance on gender-specific cut-offs on anthropometry in PLWD.