Miracle-dz/UniFAR-FTUs huggingface dataset:
HIV fragments detected in Kaposi sarcoma tumor cells in HIV-infected patients

Kaposi sarcoma (KS) is a malignant vascular neoplasm caused by KS-associated herpesvirus (KSHV) infection. HIV plays a major role in KS pathogenesis. KS in HIV usually produces more malignant features than classic KS. Despite the close KS–HIV relationship, no study has reported the existence of HIV in KS tissue. We used ddPCR to detect HIV and KSHV in HIV+ KS samples and classic KS control. We verified KS cell types through immunohistochemistry and applied hypersensitive in situ hybridization (ISH) to detect HIV and KSHV in tumor cells. Furthermore, we co-stained samples with ISH and immunohistochemistry to identify HIV and KSHV in specific cell types. Regarding pathological stages, the KS were nodular (58.3%), plaque (33.3%), and patch (8.3%) tumors. Moreover, ddPCR revealed HIV in 58.3% of the KS samples. ISH revealed positive Pol/Gag mRNA signals in CD34 + tumor cells from HIV + patients (95.8%). HIV signals were absent in macrophages and other inflammatory cells. Most HIV + KS cells showed scattered reactive particles of HIV and KSHV. We demonstrated that HIV could infect CD34 + tumor cells and coexist with KSHV in KS, constituting a novel finding. We hypothesized that the direct KSHV–HIV interaction at the cellular level contributes to KS oncogenesis.
CT imaging features regarding ground-glass nodules and solid lesions reflect prognostication of synchronous multiple lung adenocarcinoma

The prognosis of synchronous multiple lung adenocarcinoma (SMLA) dramatically differs due to its nature of multiple primaries or intrapulmonary metastases. This study aimed to assess computed tomography (CT)-reflected SMLA features regarding ground-glass nodules (GGNs) and solid lesions and their correlation with prognostication. One seventy eight SMLA patients who underwent surgical resection were reviewed. According to preoperative CT features, patients were categorized as: multiple GGN (MG) group: MGs without solid lesions; solid plus GGN (SPG) group: one solid lesion and at least one GGN; multiple solid (MS) group: MS lesions, with or without GGNs. Clinical characteristics, disease-free survival (DFS), and overall survival (OS) were retrieved. Largest tumor size (P < .001) and lymph-node metastasis prevalence (P < .001) were different among three groups, which were highest in the MS group, followed by the SPG group, and lowest in the MG group. Besides, the dominant tumor subtype also varied among the three groups (P < .001), while no difference in other clinical characteristics was discovered. DFS was more deteriorative in the MS group compared to the SPG group (P = .017) and MG group (P < .001), while of no difference between the SPG group and MG group (P = .128). Meanwhile, OS exhibited similar treads among the three groups. Besides, after multivariate Cox analyses adjustment, MS versus MG independently correlated with DFS (P = .030) and OS (P = .027), but SPG versus MG did not. In conclusion, preoperative CT-imaging MS lesions reflect advanced disease features and poor prognosis compared to MG and solid lesion plus GGN in SMLA patients who underwent surgical resection.
The Additive Impact of Transbronchial Cryobiopsy Using a 1.1-mm Diameter Cryoprobe on Conventional Biopsy for Peripheral Lung Nodules

Purpose The diagnostic yield of transbronchial biopsy (TBB) using radial probe endobronchial ultrasound (RP-EBUS) is 71%, which is lower than that of transthoracic needle biopsy. We investigated the performance and safety of sequential transbronchial cryobiopsy (TBC) using a novel 1.1-mm diameter cryoprobe, after conventional TBB using RP-EBUS for the diagnosis of peripheral lung lesions (PLLs). Materials and Methods From April 2021 to November 2021, 110 patients who underwent bronchoscopy using RP-EBUS for the diagnosis of PLL ≤ 30 mm were retrospectively included in our study. All records were followed until June 2022. Results The overall diagnostic yield of combined TBB and TBC was 79.1%, which was higher than 60.9% of TBB alone (p=0.005). The diagnostic yield of sequential TBC was 65.5%, which increased the overall diagnostic yield by 18.2%. The surface area of tissues by TBC (mean area, 18.5 mm2) was significantly larger than those of TBB by 1.5-mm forceps (3.4 mm2, p < 0.001) and 1.9-mm forceps (3.7 mm2, p=0.011). In the multivariate analysis, PLLs with the longest diameter of ≤ 22 mm were found to be related to additional diagnostic benefits from sequential TBC (odds ratio, 3.51; 95% confidence interval, 1.043 to 11.775; p=0.042). Complications were found in 10.5% of the patients: pneumothorax (1.0%), infection (1.0%), and significant bleeding (8.6%). None of the patients developed any life-threatening complications. Conclusion Sequential TBC with a 1.1-mm cryoprobe improved the performance of conventional TBB using RP-EBUS without serious complications.
Dynamical multistability in a quantum-dot laser

We study a model for a single-atom laser implemented in a quantum dot spin valve. A resonator, consisting of a mechanical oscillator or a microwave cavity mode, interacts with two spin levels in a dot in contact with two ferromagnetic leads of antiparallel polarization. We prove that such a system encodes a single-atom laser where high-efficiency pumping is achieved by spin-polarized tunneling electrons. Moreover, the oscillator amplitude displays a multi-peaked distribution. This lasing multistability regime provides an opportunity to explore the effects of coherent spin-oscillator dynamics beyond the usual rotating-wave approximation at relatively weak couplings. The multistability manifests itself directly in the charge current flowing through the dot, switching between distinct current levels corresponding to the different states of oscillation.
Vertical distributions of dolphinfish (Coryphaena hippurus) in the Eastern Pacific Ocean suggest variability in potential associations with floating objects

Floating objects play a pivotal role in pelagic ecosystems by serving as shelters, meeting points, cleaning stations, nurseries, and feeding grounds. The abundance of these objects is increasing globally in the form of flotsam, plastics, discarded or lost fishing gear, and fish aggregating devices (FADs) deployed by commercial fisheries. However, it is difficult to measure how often and in what ways fish interact with floating objects in pelagic environments. Dolphinfish (Coryphaena hippurus) is prevalent among the fish species that associate with floating objects, but the extent to which dolphinfish utilize them is unclear. This study applies existing knowledge of FAD-associated dolphinfish diving behavior to identify periods of potential association with floating objects in a remote telemetry dataset of 23 fish with a total of 678 days at liberty spanning two distinct regions within the Eastern Pacific Ocean. Fish inhabiting waters off the western coast of Baja California Sur, Mexico spent significantly more time exhibiting behavior indicative of association with floating objects than those off the coast of Oaxaca, Mexico. When not exhibiting this behavior, dolphinfish in both regions occupied similar vertical habitats, with western Baja fish utilizing more of the water column than Oaxaca fish. Observed regional differences in behavior were coincident with regional differences in size (Oaxaca fish fork lengths ranged from 103 to 118 cm (mean = 110 cm), while Baja fish ranged from 85 to 106 cm (mean = 93 cm)). Although larger fish in the Baja region displayed behavior consistent with smaller Baja fish, future studies should investigate whether the observed regional differences are due to (i) size, (ii) sex, (iii) oceanography, or (iv) availability of floating objects. Dolphinfish are an important mid-trophic level predator and potentially sustainable fishery resource. Understanding their behavior and use of floating objects is of both ecological and economic importance–particularly in the context of expanding international FAD-based fisheries. Our study suggests dolphinfish spend a large amount of their time exhibiting potential floating object associated behavior, and this could influence their population structure and growth.
Modifying factors of PD‐L1 expression on tumor cells in advanced non‐small‐cell lung cancer

Programmed death ligand‐1 (PD‐L1) expression predicts immunotherapy utility in nononcogenic addictive lung adenocarcinoma (ADC). However, its reproducibility and reliability may be compromised outside clinical trials. This study aimed to evaluate factors associated with PD‐L1 expression in lung ADC.
Understanding motivations and deterrents for COVID-19 vaccination among US working adults: A mixed method approach

ABSTRACT COVID-19 vaccines have been developed and administered in the United States. Despite evidence from clinical trials for the effectiveness of the COVID-19 vaccines, many individuals are still hesitant or even unwilling to receive one. The purposes of this study are (1) to examine characteristics associated with those willing and unwilling to receive a COVID-19 vaccine and (2) to illuminate the reasons behind their willingness and unwillingness to receive the vaccine using both quantitative and qualitative data. Data collected from 505 US working adults showed that several demographic variables (i.e. education, the size of their organization, the number of dependents, political orientation, and religion) and influence sources (i.e. family members, workplace leaders, political leaders, social media influencers, and healthcare workers) significantly correlated with people’s willingness/unwillingness to receive a COVID-19 vaccine. Furthermore, protecting oneself was the most common reason cited by participants for willingness to get the vaccine, while being concerned about vaccine side effects was the most frequently given reason for being unwilling to receive a COVID-19 vaccine. This study expands our current understanding of the COVID-19 vaccine motivators and intention factors. Practically, the findings can help develop health campaign messages effectively target working adults who are unwilling to receive the COVID-19 vaccines and ultimately increase the vaccination rate in the United States.
ALMA 12CO (J = 1–0) imaging of the nearby galaxy M 83: Variations in the efficiency of star formation in giant molecular clouds

We present results of the $^{12}$CO (1--0) mosaic observations of the nearby barred-spiral galaxy M83 obtained with the Atacama Large Millimeter/submillimeter Array (ALMA). The total flux is recovered by combining the ALMA data with single-dish data obtained using the Nobeyama 45-m telescope. The combined map covers a $\sim$13 kpc$^{2}$ field that includes the galactic center, eastern bar, and spiral arm with a resolution of \timeform{2''. 03} $\times$ \timeform{1''. 1} ($\sim$45 pc $\times$ $\sim$25 pc). With a resolution comparable to typical sizes of giant molecular clouds (GMCs), the CO distribution in the bar and arm is resolved into many clumpy peaks that form ridge-like structures. Remarkably, in the eastern arm, the CO peaks form two arc-shaped ridges that run along the arm and exhibit a distinct difference in the activity of star formation: the one on the leading side has numerous HII regions associated with it, whereas the other one on the trailing side has only a few. To see whether GMCs form stars with uniform star formation efficiency (SFE) per free-fall time (SFEff), GMCs are identified from the data cube and then cross-matched with the catalog of HII regions to estimate the star formation rate for each of them. 179 GMCs with a median mass of 1.6 $\times$ 10$^{6}$ $M_{\odot}$ are identified. The mass-weighted average SFEff of the GMCs is $\sim$9.4 $\times$ 10$^{-3}$, which is in agreement with models of turbulence regulated star formation. Meanwhile, we find that SFEff is not universal within the mapped region. In particular, one of the arm ridges shows a high SFEff with a mass-weighted value of $\sim$2.7 $\times$ 10$^{-2}$, which is higher by more than a factor of 5 compared to the inter-arm regions. This large regional variation in SFEff favors the recent interpretation that GMCs do not form stars at a constant rate within their lifetime.
Skipping orbits and enhanced resistivity in large diameter InAs/GaSb antidot lattices

We investigated the magnetotransport properties of high-mobility InAs/GaSb antidot lattices. For samples with large antidot diameter, we found a broad maximum of classical origin around 2.5 T in addition to the usual commensurability features at low magnetic fields. The broad maximum can be ascribed to a class of orbits involving multiple reflections on a single antidot. This is shown by both a simple transport calculation based on a classical Kubo formula and an analysis of the Poincare sections at different magnetic-field values. At low temperatures we observe weak 1/B- periodic oscillations superimposed on the classical maximum.
Organisation of potential vorticity on the mesoscale during deep moist convection

Potential vorticity (PV) and its conservation principle elegantly describe large-scale atmospheric dynamics. On the mesoscale, however, PV has received less attention. We describe the characteristics of PV on the convective weather scale (≈10 km) as simulated by the non-hydrostatic numerical weather prediction model COSMO-DE. Two weather cases with a different synoptic background are analysed, on 5 June 2011 and 22 June 2011. Composites of PV and other quantities like wind velocity around storm updrafts are calculated to test consistency of PV anomalies associated with storm updrafts. For the frontal case on 22 June bands of positive and negative PV form approximately along the direction of the wind shear. A possible explanation of these elongated PV bands is the preferential generation of new cells downshear of old cells in an atmosphere with moderate to high vertical wind shear. The PV dipoles are much less consistent in direction in the 5 June case with localised deep convection. For both cases, the wind anomalies around the convective PV anomalies are consistent with the flow around synoptic PV anomalies. The coherent PV anomalies around storm updrafts motivate the use of PV-thinking on smaller scales.
MC2: A Deeper Look at ZwCl 2341.1+0000 with Bayesian Galaxy Clustering and Weak Lensing Analyses

ZwCl 2341.1+0000, a merging galaxy cluster with disturbed X-ray morphology and widely separated (∼3 Mpc) double radio relics, was thought to be an extremely massive ( 10 – 30 × 10 14 M ⊙ ) and complex system, with little known about its merger history. We present JVLA 2–4 GHz observations of the cluster, along with new spectroscopy from our Keck/DEIMOS survey, and apply Gaussian Mixture Modeling to the three-dimensional distribution of 227 confirmed cluster galaxies. After adopting the Bayesian Information Criterion to avoid overfitting, which we discover can bias high the total dynamical mass estimates, we find that a three-substructure model with a total dynamical mass estimate of 9.39 ± 0.81 × 10 14 M ⊙ is favored. We also present deep Subaru imaging and perform the first weak lensing analysis on this system, obtaining a weak lensing mass estimate of 5.57 ± 2.47 × 10 14 M ⊙ . This is a more robust estimate because it does not depend on the dynamical state of the system, which is disturbed due to the merger. Our results indicate that ZwCl 2341.1+0000 is a multiple merger system comprised of at least three substructures, with the main merger that produced the radio relics occurring near the plane of the sky, and a younger merger in the north occurring closer to the line of sight. Dynamical modeling of the main merger reproduces observed quantities (relic positions and polarizations, subcluster separation and radial velocity difference), if the merger axis angle of ∼ 10 − 6 + 34 degrees and the collision speed at pericenter is ∼ 1900 − 200 + 300 km s−1.
Weak gravity conjecture in the asymptotical dS and AdS background

The cosmological observations provide a strong evidence that there is a positive cosmological constant in our universe and thus the spacetime is asymptotical de Sitter space. The conjecture of gravity as the weakest force in the asymptotical dS space leads to a lower bound on the U(1) gauge coupling g, or equivalently, the positive cosmological constant gets an upper bound rho(V) <= g(2)M(p)(4) in order that the U(1) gauge theory can survive in four dimensions. This result has a simple explanation in string theory, i. e. the string scale root alpha' should not be greater than the size of the cosmic horizon. Our proposal in string theory can be generalized to U(N) gauge theory and gives a guideline to the microscopic explanation of the de Sitter entropy. The similar results are also obtained in the asymptotical anti-de Sitter space.
Estrogen regulates divergent transcriptional and epigenetic cell states in breast cancer

Abstract Breast cancers are known to be driven by the transcription factor estrogen receptor and its ligand estrogen. While the receptor's cis-binding elements are known to vary between tumors, heterogeneity of hormone signaling at a single-cell level is unknown. In this study, we systematically tracked estrogen response across time at a single-cell level in multiple cell line and organoid models. To accurately model these changes, we developed a computational tool (TITAN) that quantifies signaling gradients in single-cell datasets. Using this approach, we found that gene expression response to estrogen is non-uniform, with distinct cell groups expressing divergent transcriptional networks. Pathway analysis suggested the two most distinct signatures are driven separately by ER and FOXM1. We observed that FOXM1 was indeed activated by phosphorylation upon estrogen stimulation and silencing of FOXM1 attenuated the relevant gene signature. Analysis of scRNA-seq data from patient samples confirmed the existence of these divergent cell groups, with the FOXM1 signature predominantly found in ER negative cells. Further, multi-omic single-cell experiments indicated that the different cell groups have distinct chromatin accessibility states. Our results provide a comprehensive insight into ER biology at the single-cell level and potential therapeutic strategies to mitigate resistance to therapy.
Weak-value amplification of photon-number operators in the optomechanical interaction

An experimental proposal is presented in which dark port post-selection together with weak measurements are used to enlarge the radiation pressure effect of a single photon on a mechanical oscillator placed in the middle of a Fabry-Perot cavity and initialized in the ground state. By preparing and post-selecting the photon (the system) in two quasi orthogonal states, the weak value of the radiation force operator can lie outside the eigenvalue spectrum, producing a large shift on the wave function of the mechanical oscillator (the measuring device) in the position representation. Consequently, the effect of a single photon on the average position of the oscillator in its final state can be amplified as compared to the effect caused by a photon without post-selection, i.e. only pre-selected. The strong measurement scenario is also analyzed. In this case, a higher amplification effect is achieved and the mean position of the oscillator reaches the level of the zero-point fluctuation, but the back-action on the system is increased and the post-selection probabilities are smaller.
Representations and Identities for Homogeneous Technologies

Using up to nine different ways to represent homogeneous technologies with decreasing returns to scale, this article presents and proves identities between those different representations of such technologies, outlining the homogeneity properties of each representation. These identities, which allow to shift from one representation of a technology to another -- and which are summarized in a matrix of identities -- can be useful since they provide a tool to obtain explicit functional forms for homogeneous technologies. They can also be useful to simplify computational procedures when different representations of a technology are needed. Finally, the article also refers explicitly to some aspects of producer theory that are often neglected or treated in a marginal way in the literature, such as the inverse supply, the non conditional cost and the inverse input demands functions.
Do people use methods or tricks to fall asleep? A comparison between people with and without chronic insomnia

The present study estimated the frequency of using methods or tricks to fall asleep in the general Norwegian population. Further, people with chronic insomnia were compared with people without chronic insomnia. A representative sample of 1028 participants aged 18 years or older completed a web‐based survey. The response rate was 33.5%. Insomnia symptoms were assessed with the validated Bergen Insomnia Scale, and chronic insomnia based on ICSD‐3/DSM‐5 criteria. Data were analysed with chi‐square tests and logistic regression with adjustment for sex, age, education, and circadian preference. The results showed that 34.3% reported using a method or a trick to fall asleep, with relaxation exercises/breathing exercises being the most common. More females (39.5%) compared with males (29.1%) reported the use of a method/trick to fall asleep with an adjusted odds ratio (aOR) of 1.44. Chronic insomnia was reported by 24.9%, and clearly associated with higher use of such methods/tricks (53.7%; aOR = 3.49). Among the participants without chronic insomnia, 28.1% reported using methods/tricks to fall asleep. In conclusion, most people do not use methods or tricks to fall asleep, but chronic insomnia was associated with a higher frequency of such use. Still, since methods/tricks were also used by some participants without chronic insomnia, this may suggest that, for some people, this strategy may be effective or at least does not seem to disrupt the sleep onset process.
Residential parenting services: An integrative literature review of characteristics, service usage and parent and staff perspectives

The primary aim of the review was to provide an overall assessment of residential parenting services in Australia, by describing the characteristics of infants and parents using residential parenting services, their prior service use and reasons for admission, referral pathways for access and parenting and infant outcomes. The secondary aims were to explore parent and staff perception of the programmes.