Miracle-dz/UniFAR-FTUs huggingface dataset:
Current Animal Model Systems for Ovarian Aging Research

Ovarian aging leads to menopause, loss of fertility and other disorders in multiple organs, which brings great distress to women. For ethical reasons, it is impossible to use humans as direct study subjects for aging research. Therefore, biomedical researchers have employed different non-human organisms to study ovarian aging, including worms, fruit flies, fishes, amphibians, birds, mice, rats, cavies, rabbits, pigs, sheep, cows, horses, monkeys, and apes. Because each of these model organisms has its own features, multiple factors, such as size, anatomical structure, cost, ease of operation, fertility, generation time, lifespan, and gene heredity, should be carefully considered when selecting a model system to study ovarian aging. An appropriate model organism would help researchers explore the risk factors and elucidate molecular mechanisms underlying declined ovarian functions, which might be conducive to preventing or delaying the ovarian aging process. This article will offer an overview on several currently available and commonly used model organisms for ovarian aging research by comparing their pros and cons. In doing so, we hope to provide useful information for ovarian aging researchers.
Impact of Load Variation on the Accuracy of Gait Recognition from Surface EMG Signals

As lower-limb exoskeleton and prostheses are developed to become smarter and to deploy man–machine collaboration, accurate gait recognition is crucial, as it contributes to the realization of real-time control. Many researchers choose surface electromyogram (sEMG) signals to recognize the gait and control the lower-limb exoskeleton (or prostheses). However, several factors still affect its applicability, of which variation in the loads is an essential one. This study aims to (1) investigate the effect of load variation on gait recognition; and to (2) discuss whether a lower-limb exoskeleton control system trained by sEMG from different loads works well in multi-load applications. In our experiment, 10 male college students were selected to walk on a treadmill at three different speeds (V3 = 3 km/h, V5 = 5 km/h, and V7 = 7 km/h) with four different loads (L0 = 0, L20 = 20%, L30 = 30%, L40 = 40% of body weight, respectively), and 50 gait cycles were performed. Back propagation neural networks (BPNNs) were used for gait recognition, and a support vector machine (SVM) and k-nearest neighbor (k-NN) were used for comparison. The result showed that (1) load variation has significant effects on the accuracy of gait recognition (p < 0.05) under the three speeds when the loads range in L0, L20, L30, or L40, but no significant impact is found when the loads range in L0, L20, or L30. The least significant difference (LSD) post hoc, which can explore all possible pair-wise comparisons of means that comprise a factor using the equivalent of multiple t-tests, reveals that there is a significant difference between the L40 load and the other three loads (L0, L20, L30), but no significant difference was found among the L0, L20, and L30 loads. The total mean accuracy of gait recognition of the intra-loads and inter-loads was 91.81%, and 69.42%, respectively. (2) When the training data was taken from more types of loads, a higher accuracy in gait recognition was obtained at each speed, and the statistical analysis shows that there was a substantial influence for the kinds of loads in the training set on the gait recognition accuracy (p < 0.001). It can be concluded that an exoskeleton (or prosthesis) control system that is trained in a single load or the parts of loads is insufficient in the face of multi-load applications.
Effect of Rosemary Extract on Lipid Oxidation, Fatty Acid Composition, Antioxidant Capacity, and Volatile Compounds of Salted Duck Eggs

Abstract The purpose of our study was to determine the impact of rosemary extract in duck eggs, as determined by in vitro antioxidant capacity, lipid oxidation, fatty acid profiles, and flavor analyses. Three groups of salted duck eggs were compared: A control group and group enriched with 0.1% and 0.5% (w/v) rosemary extracts for 28 days of salting. In a time-dependent manner, the radical scavenging activity and reduction power of eggs with 0.5% (w/v) rosemary extract were significantly higher those of the control at 28 days after salting. The fatty acid profiles of salted egg were significantly affected by rosemary extract and salting time. Palmitic acid was the most abundant fatty acid in salted egg treated with rosemary extract, followed by linoleic acid and arachidonic acid. Furthermore, the treated eggs contained more docosahexaenoic acid than the control ones. And the treated eggs also have a considerable impact on the lipid oxidation process (primary and secondary oxidation). As a result, rosemary extract can be used as a natural antioxidant spice to prevent oxidation and extend the shelf life of eggs during storage. Furthermore, flavor research using solid phase microextraction - gas chromatography - mass spectrometry and an electronic nose demonstrated that adding rosemary extract to salted eggs could give them a distinct flavor.
Beverage and soft drink consumption by adolescents from a public school

Objective: To evaluate the consumption of beverage and soft drinks by adolescents of a public school in Sao Paulo, Brazil. Methods: 71 adolescents (aged between 14 to 17 years old) from both genders, attending a technical school in the metropolitan area of Sao Paulo, answered the following questions: the kind of beverage taken during meals, the places where soft drinks were consumed and the main reason related to this intake. Results: The most frequent consumed beverage was the industrialized fruit juice (38.1%), followed by regular soft drinks (28.6%) and natural fruit juices (22.2%). The main place where soft drinks were consumed was home (38.2%), followed by school (22.1%). The main reason associated with soft drink intake was the flavor (75.4%). Conclusions: Sweet beverage intake was frequent among adolescents, specially soft drinks. These beverages are avail- able and consumed at home and at school, and they were considered tasteful. Nutritional education programs should discuss how to prioritize the intake of other beverages and how to control the sale of these products at schools, aiming to stimulate the intake of more healthy beverages by adolescents.
Social jet lag and eating styles in young adults

ABSTRACT Social jet lag refers to circadian misalignment that occurs when people shift their sleep schedules from weekdays to weekends. Social jet lag is linked with numerous negative health outcomes, with emerging research connecting social jet lag to increased consumption of unhealthy foods. Existing research has not yet examined the associations between social jet lag and eating styles (e.g., emotional eating). Emotional eating and loss of control over eating are problematic eating styles which may lead to overeating and weight gain. Conversely, intuitive eating is associated with positive health outcomes such as lower risk of obesity. The present study examined social jet lag and eating styles in a young adult sample, as emerging adulthood is an important developmental time period for establishing healthy sleep and eating habits. Results of the current study indicated that in a sample of 372 American undergraduate participants, social jet lag significantly predicted lower intuitive eating (β = −.129, p = .012) and greater emotional eating (β = .12, p = .022) when controlling for age, sex, and chronotype. It was marginally predictive of loss of control over eating (β = .102, p = .050). Sleep quantity on weekdays (not weekends) also significantly predicted intuitive eating (p = .017) and loss of control over eating (p = .044), and sleep quality significantly predicted intuitive eating (p < .001), emotional eating (p < .001), and loss of control over eating (p < .001). These findings extend our understanding of the relationship between social jet lag and eating styles in college students. Addressing social jet lag in this population is an important consideration for their cardiometabolic health and for reducing weight fluctuations.
Effects of cerebral amyloid angiopathy on the brain vasculome

β‐amyloid (Aβ) deposits in brain blood vessel walls underlie the vascular pathology of Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). Growing evidence has suggested the involvement of cerebrovascular dysfunction in the initiation and progression of cognitive impairment in AD and CAA patients. Therefore, in this study, we assessed the brain vasculome in a mouse model in order to identify cerebrovascular pathways that may be involved in AD and CAA vascular pathogenesis in the context of aging. Brain endothelial cells were isolated from young and old wild‐type mice, and young and old transgenic mice expressing Swedish mutation in amyloid precursor protein and exon 9 deletion in presenilin 1 (APPswe/PSEN1dE9). Microarray profiling of these endothelial transcriptomes demonstrated that accumulation of vascular Aβ in the aging APPswe/PSEN1dE9 mouse is associated with impaired endothelial expression of neurotransmitter receptors and calcium signaling transductors, while the genes involved in cell cycle and inflammation were upregulated. These results suggest that the vascular pathology of AD and CAA may involve the disruption of neurovascular coupling, reactivation of cell cycle in quiescent endothelial cells, and enhanced inflammation. Further dissection of these endothelial mechanisms may offer opportunities to pursue therapies to ameliorate vascular dysfunction in the aging brain of AD and CAA patients.
Eccentric Force Velocity Profiling: Motor Control Strategy Considerations and Relationships to Strength and Jump Performance

Abstract Barker, L, Siedlik, J, Magrini, M, Uesato, S, Wang, H, Sjovold, A, Ewing, G, and Harry, JR. . Eccentric force velocity profiling: motor control strategy considerations and relationships to strength and jump performance. J Strength Cond Res 37(3): 574–580, 2023—Currently, no studies exist on the eccentric force-velocity profile (eFVP) during drop landings from increasing drop heights, which may reveal an athlete's braking capacity and control strategies. Therefore, the purpose of this study was to assess the eFVP during bilateral drop landings from increasing drop heights. A secondary purpose was to explore and determine relevant relationships between the eFVP and common metrics like relative strength and jumping performance. Overall, 19 recreationally trained athletes from the university completed a 1-reptition maximum back squat, countermovement jumps, squat jumps, drop jumps, and drop landings from 0.3 to 1.52-m box heights in 0.15-m increments. Average force and velocity from the peak drop landing trial was used to generate an eFVP. The mean linear eFVP was −6.65x + 14.73, and the mean second order polynomial eFVP was −1.37x2 − 25.84x + 0.17. The second-order polynomial fit the data better with large effect (dunb = 1.05, p < 0.05). No significant correlations between the eFVP coefficients and the strength and jumping measurements were observed. Future research could investigate how training can influence the eFVP. Eccentric force production during landing may be a unique quality that requires specific development strategies, such has fast or slow eccentric training.
Reduced left ventricular function and sustained hypertension in women seven years after severe preeclampsia

Abstract Objective. To study left ventricular (LV) function and blood pressure (BP) at a long-term follow-up in women after severe pre-eclampsia. Design. In this single-centre, cross-sectional study, 96 patients were eligible for inclusion. LV function was examined by transthoracic echocardiography including tissue Doppler echocardiography and speckle tracking. BP was measured at rest using repeated non-invasive techniques. Results. We compared 36 patients with early-onset and 33 patients with late-onset pre-eclampsia with 28 healthy controls. Mean age (40 ± 3 years) and median time since delivery (7 ± 2 years) were similar across the study groups. The patients had 18% higher systolic BP (139 ± 15 mmHg) and 24% higher diastolic BP (87 ± 19 mmHg) than controls (p < .01). Hypertension was present in 23 patients (33%), where the estimated LV mass was 16% higher (p = .05) than in controls. The LV ejection fraction was 19% lower in the early-onset group (51 ± 4%; p = .01) and 14% lower in the late-onset group (54 ± 6; p = .04) compared with controls. LV global longitudinal strain was 18% lower in the patient group (–17.7 ± 2.1%) compared with controls (p = .01). Indicative of a more restrictive filling pattern, the diastolic indices showed a lower e′ mean (p < .01) and subsequently higher E/e′ ratio (p < .01). There were no significant differences in BP, systolic or diastolic function indices between the patient groups. Conclusion. We found sustained hypertension, higher LV mass and reduced LV systolic and diastolic function 7 y after severe pre-eclampsia. Our findings emphasize the importance of early risk stratification and clinical counselling, and follow-up for such cases.
The effect of antiretroviral therapy with high central nervous system penetration on HIV-related cognitive impairment: a systematic review and meta-analysis

ABSTRACT Chronic complications are a significant concern for people living with HIV/AIDS (PLWHA) infection. HIV-associated neurocognitive disorders (HAND) are prevalent in PLWHA. Yet, the efficacy of medications that penetrate the central nervous system (CNS) at preventing or slowing the progression of HAND remains largely unknown. The objective of this study was to determine whether high CNS penetration effectiveness (CPE) regimens improve neurocognitive test scores in PLWHA on combined antiretroviral therapy (cART). Primary literature evaluating cognitive outcomes based on CPE score of cART regimens in PLWHA was assembled from PubMed/Medline and EMBASE. Both randomized controlled trials and observational studies with at least 12 weeks of follow-up were included. A meta-analysis was conducted to calculate the standardized mean difference. Eight trials including a total of 3,303 patients with 13,103 person-years of follow-up were included in the systematic review. Four trials (n = 366 patients) met our inclusion criteria and were included in the meta-analysis. In the meta-analysis, HIV regimens with a high CPE score did not affect NPZ-4 or GDS scores (standardized mean difference (SMD) 0.10, 95% CI −0.19, 0.38; I2 = 26%). Future studies with larger sample sizes are warranted to prospectively evaluate the relationship between CPE and progression of HAND.
A Novel Multilevel Solar Panel System: Implementation and Verification

A prototype multilevel solar panel system with an automated solar tracker has been developed for its use in urban areas. The system consists of three solar panels stacked one above another at some fixed distance to minimize the floor area and shifted horizontally from one another to avoid the shading of the lower panels. Experimental results show that the developed system can harness about 23% more energy while occupying 33 % less floor area than that by the conventional fixed panel system of same size. The proposed system is easy to construct and implement, takes less floor space and will be effective for urban areas where not only electricity is in short supply, availability of roof top space to install solar panels is also very limited.
Identification of Three Different Phenotypes in Anti–Melanoma Differentiation–Associated Gene 5 Antibody–Positive Dermatomyositis Patients: Implications for Prediction of Rapidly Progressive Interstitial Lung Disease

There is substantial heterogeneity among the phenotypes of patients with anti–melanoma differentiation–associated gene 5 antibody–positive (anti‐MDA5+) dermatomyositis (DM), hindering disease assessment and management. This study aimed to identify distinct phenotype groups in patients with anti‐MDA5+ DM and to determine the utility of these phenotypes in predicting patient outcomes.
Communication partners experiences of communicating with adults with severe/profound intellectual disability through augmentative and alternative communication: A mixed methods systematic review

People with severe/profound intellectual disability experience challenges in communicating and require their communication partners to adapt to their means of communication. Augmentative and Alternative Communication (AAC) is recognised as a potential means to meet their communication needs. Interventions need to be aimed at both the individual and their communication partners. We conducted a mixed methods systematic review of the literature to synthesise evidence on communication partners experience of communicating with adults with severe/profound intellectual disability through AAC. Eight publications met the inclusion criteria, they underwent thematic synthesis where four themes emerged. A shared commitment to communication partnership is fundamental for the effective and efficient use of AAC. However, there was a disconnect between communication partners perceptions of their roles and responsibilities. This review prompts further research to explore communication partners perceptions of their roles and responsibilities in the use of AAC with people with severe/profound intellectual disabilities.
BET-Independent Murine Leukemia Virus Integration Is Retargeted In Vivo and Selects Distinct Genomic Elements for Lymphomagenesis

In this study, we have shown that the in vivo replication of murine leukemia virus happens independently of BET proteins, which are key host determinants involved in retroviral integration site selection. This finding opens a new research line in the discovery of alternative viral or host factors that may complement the dominant host factor. ABSTRACT Moloney murine leukemia virus (MLV) infects BALB/c mice and induces T-cell lymphoma in mice. Retroviral integration is mediated by the interaction of the MLV integrase (IN) with members of the bromodomain and extraterminal motif (BET) protein family (BRD2, BRD3, and BRD4). The introduction of the W390A mutation into MLV IN abolishes the BET interaction. Here, we compared the replication of W390A MLV to that of wild-type (WT) MLV in adult BALB/c mice to study the role of BET proteins in replication, integration, and tumorigenesis in vivo. Comparing WT and W390A MLV infections revealed similar viral loads in the blood, thymus, and spleen cells. Interestingly, W390A MLV integration was retargeted away from GC-enriched genomic regions. However, both WT MLV- and W390A MLV-infected mice developed T-cell lymphoma after similar latencies represented by an enlarged thymus and spleen and multiorgan tumor infiltration. Integration site sequencing from splenic tumor cells revealed clonal expansion in all WT MLV- and W390A MLV-infected mice. However, the integration profiles of W390A MLV and WT MLV differed significantly. Integrations were enriched in enhancers and promoters, but compared to the WT, W390A MLV integrated less frequently into enhancers and more frequently into oncogene bodies such as Notch1 and Ppp1r16b. We conclude that host factors direct MLV in vivo integration site selection. Although BET proteins target WT MLV integration preferentially toward enhancers and promoters, insertional lymphomagenesis can occur independently from BET, likely due to the intrinsically strong enhancer/promoter of the MLV long terminal repeat (LTR). IMPORTANCE In this study, we have shown that the in vivo replication of murine leukemia virus happens independently of BET proteins, which are key host determinants involved in retroviral integration site selection. This finding opens a new research line in the discovery of alternative viral or host factors that may complement the dominant host factor. In addition, our results show that BET-independent murine leukemia virus uncouples insertional mutagenesis from gene enhancers, although lymphomagenesis still occurs despite the lack of an interaction with BET proteins. Our findings also have implications for the engineering of BET-independent MLV-based vectors for gene therapy, which may not be a safe alternative.
Core Genome Multilocus Sequence Typing Scheme for Improved Characterization and Epidemiological Surveillance of Pathogenic Brucella

Brucellosis poses a significant burden to human and animal health worldwide. Robust and harmonized molecular epidemiological approaches and population studies that include routine disease screening are needed to efficiently track the origin and spread of Brucella strains. ABSTRACT Brucellosis poses a significant burden to human and animal health worldwide. Robust and harmonized molecular epidemiological approaches and population studies that include routine disease screening are needed to efficiently track the origin and spread of Brucella strains. Core genome multilocus sequence typing (cgMLST) is a powerful genotyping system commonly used to delineate pathogen transmission routes for disease surveillance and control. Except for Brucella melitensis, cgMLST schemes for Brucella species are currently not established. Here, we describe a novel cgMLST scheme that covers multiple Brucella species. We first determined the phylogenetic breadth of the genus using 612 Brucella genomes. We selected 1,764 genes that were particularly well conserved and typeable in at least 98% of these genomes. We tested the new scheme on 600 genomes and found high agreement with the whole-genome-based single nucleotide polymorphism (SNP) analysis. Next, we applied the scheme to reanalyze the genome of Brucella strains from epidemiologically linked outbreaks. We demonstrated the applicability of the new scheme for high-resolution typing required in outbreak investigations as previously reported with whole-genome SNP methods. We also used the novel scheme to define the global population structure of the genus using 1,322 Brucella genomes. Finally, we demonstrated the possibility of tracing distribution of Brucella strains by performing cluster analysis of cgMLST profiles and found nearly identical cgMLST profiles in different countries. Our results show that sequencing depth of more than 40-fold is optimal for allele calling with this scheme. In summary, this study describes a novel Brucella-wide cgMLST scheme that is applicable in Brucella molecular epidemiology and helps in accurately tracking and thus controlling the sources of infection. The scheme is publicly accessible and should represent a valuable resource for laboratories with limited computational resources and bioinformatics expertise.
A Life Cycle Assessment of Biomethane Production from Waste Feedstock Through Different Upgrading Technologies

Upgrading consists of a range of purification processes aimed at increasing the methane content of biogas to reach specifications similar to natural gas. In this perspective, an environmental assessment, based on the Life Cycle Assessment (LCA) method, of different upgrading technologies is helpful to identify the environmental characteristics of biomethane and the critical steps for improvement. The aim of this work is to conduct an LCA of biomethane production from waste feedstock, using the SimaPro software. The study focuses on the comparison of several upgrading technologies (namely, membrane separation, cryogenic separation, pressure swing adsorption, chemical scrubbing, high pressure water scrubbing) and the on-site cogeneration of electricity and heat, including the environmental benefits deriving from the substitution of fossil-based products. The results show a better environmental performance of the cogeneration option in most of the impact categories. The Fossil resource scarcity is the impact category which is mainly benefited by the avoided production of natural gas, with savings of about 0.5 kg oil eq/m3 of biogas for all the investigated technologies, with an average improvement of about 76% compared to conventional cogeneration. The results show that the membrane upgrading technology is slightly more environmentally convenient than the other upgrading technologies.