Miracle-dz/UniFAR-FTUs huggingface dataset:
Pseudomonas Otitidis and Bacillus Subtilis from Saline Soil of Pakistan Exhibiting a Potential for Calcium Precipitation

Biomineralization is an important phenomenon reported in microbes of different environments. Present study deals with the analysis of mineralization potential of two salt tolerant species named IQ (Pseudomonas otitidis) and NAY (Bacillus subtilis) isolated from rhizosphere soil. These strains have the ability to show precipitation in acetate supplemented culture medium incubated for 21 days and showed positive results for urease tests. Increase in microbial precipitation was recorded in both strains. However, strain NAY (Bacillus subtilis) showed more precipitation as compared to other IQ (Pseudomonas otitidis). It was also observed that this precipitation was associated with increase in pH of the medium from 7.0 to 8.0 that started to increase on 4th day of culture incubation and increased slightly till 15 days. The FTIR analysis of the calcite crystals showed visible peaks at 711-1400 cm-1 wavelength. The visible bubbling appeared in acid test assay further confirmed the calcite precipitation. The mineral precipitation in both species under salt stress is significant for soil consolidation as well as adjusting soil pH under acidic conditions
CHARACTERIZATION OF THE SENESCENCE-ASSOCIATED PROTEASES OF THE “TIGER FLOWER” (Tigridia pavonia) L.F. DC

<p><strong>Background:</strong> <em>Tigridia pavonia</em> is a Mexican native ornamental plant that displays great beauty and color of its flower. However, its quick senescence limits its use as cut flower, since these open in the morning and close in the afternoon of the same day exhibiting wilting of tepals. <strong>Objective: </strong>In this study flower senescence process of <em>T</em>. <em>pavonia</em> was analyzed. <strong>Methodology:</strong> The expression of several proteases during senescence of tepals, ovaries and anthers was measured by SDS-PAGE and their identification was through mass spectrometry. <strong>Results:</strong> Five proteases of apparent molecular weight of 130, 90, 65, 35 and 30 kDa were expressed during flower senescence process. The 65 kDa protease was identified as cysteine-protease and had a good homology score with early responsive to dehydration protein (ERD) besides their expression coincided with the onset of visible symptoms of tepals senescence. <strong>Implications: </strong>The preliminar sequence found belonging to ERD gen indicates that it could act as the main transcription factor integrating signaling pathways that trigger flower senescence in <em>T</em>. <em>pavonia</em>. <strong>Conclusion: </strong>Flower senescence of <em>T</em>. <em>pavonia</em> is mainly associated with the expression of cysteine-proteases. One of them is possibly associated with programmed cell death in tepals. </p>
Microbial diversity of Indigenous microorganism communities from different agri-ecosystems in Soc Trang province, Vietnam

Indigenous microorganism (IMO) has great potential for agricultural uses since they have high ability in biodegradation, nitrogen fixation, phosphate solubilization, plant growth hormone production as well as bio-control. This study aimed to determine the presence of some different major groups of microbes in IMO from different agri-ecosystem habitats like bacteria, fungi, actinomyces, Salmonella, Shigella, E. coli, and Coliform. The presence of bacteria, actinomyces, and fungi of IMO samples was identified by 27F/1492R, 243F/1378R and ITS1F/ITS4R primers, respectively. Numbers of bacteria, fungi, and actinomyces were determined by the plate counting method on TSA, PDA and Starch media, respectively. The numbers of Salmonella sp. and Shigella sp. were determined by the plate counting method on selective Salmonella and Shigella agar (SS agar) after incubation for 48 hours at 37oC while the density of Coliforms sp. and E.coli were counted by the Most Probable Number method (MPN). The results of the study showed that 3 major groups of microbes including bacteria, fungi, and actinomyces in 14 collected IMO samples were detected genetically. Moreover, bacterial numbers were dominated and ranged from 106 to 109 cfu/g IMO samples while the density of fungi and actinomyces were lower and varied from 105 to 107 cfu/g IMO sample. Interestingly, all surveyed IMO samples did not contain any human disease pathogens such as Salmonella, Shigella, Coliforms and E. coli. These results imply that collected IMO contains a high diversity of major groups of microbes and can be used as safe bio-stimulants for clean vegetable production.
Severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) detection pitfalls

The Severe Acute Respiratory Syndrome (CoVID 19) provoked by Coronavirus 2 (SARS CoV 2) require science-based responses. The aim of this work is to assess pitfalls found during the search of viral genomes due to sampling timing, swabbing, storage, heat-infectivity inactivation and further sample processing. According to several meta-analysis, on the day of symptom onset, the median false-negative rate is estimated to be 38% and decreased to 20% on day 8 (3 days after symptom onset) then increased to 66% on day 21 suggesting that rRT-PCRs adds little information immediately after exposure. RNA isolation from samples requires cautious handling using RNase-free solutions, pipet tips and glassware. The rRT PCR detection limits are estimated between 39 and 779 copies/mL but 3000 to 20.000 copies/ml for the antigen test. External cross contamination by imperceptible splatting requires risk management integrating the Pharmacopoeias by processing at least 10 negative contiguous to 10 positive controls in each sennries of 100 tests. For Ct >34 it was suggested no transmissible disease. The detection of antibodies one month or later after clinical signs may confirm positivity. Lack of immune response in non-immune compromised asymptomatic people may invalidate positivity. False positive disrupts efficiency for containing infections and leads to societal anxiety undermining health workforce. Because spurious methods create confusion, each step of diagnosis requires quality-control and risk assessment, knowing that rRT PCRs amplify more than 10.000 million times the signal of 1 viral element.
Mass Trapping Technique Using Pheromones: A Standalone Method for Management of Diamondback Moth, Plutella Xylostella (Linnaeus) (Plutellidae: Lepidoptera) in Cabbage

The diamondback moth (DBM), Plutella xylostella(Linnaeus) (Plutellidae: Lepidoptera) is found throughout the world and considered as the most destructive insect pest of cruciferous crops, particularly cabbage, broccoli and cauliflower. An attempt was made to evaluate the effectiveness of the pheromones in combination with need based insecticides under farmer’s field conditions. Trap density study results indicated that the highest number of moths trapped with highest percent population reduction was recorded in both Bangalore and Kolar with trap density @ 40 traps/acre with a highest mean head weight and yield. The data on mass trapping as a standalone method in season-I revealed that highest number of moths was trapped (675 moths), resulted in 73.96 percent reduction in larva and pupal numbers over control compared to farmer’s practice (46.11 percent). Highest number (Mean) of larvae and pupae (3.68) was observed in control plot compared to farmer’s practice plot (1.67) and standalone plot (0.89). In season-II, highest number of moths was trapped (13935 moths) in mass trapping operated plot, which had resulted 51.42 percent reduction in larva and pupal numbers over control compared to farmer’s practice with only 39.04 percent population reduction. Highest number of larvae and pupae (5.82) was observed in control plot compared to farmer’s practice plot (3.16) and mass trapped plot (3.04). Highest percent population reduction of DBM over control was observed in mass trapping as a standalone method compared to farmer’s practice. Pheromone deployed plots had significantly increased Cotesia plutellae population when compared to farmer’s practice. The C: B ratio of pheromone installed plots in both the seasons was found to be higher compared to farmer’s practice.
Challenges of clinical education in the operating room from the teachers' viewpoint: A qualitative study

Introduction: Clinical education is an ever-lasting requirement in educational settings for students and faculty. However, it is associated with numerous challenges that can have an impact on the efficacy of both the students and the teachers. The challenge can be more prominent in specialized environments such as the operating room. In this qualitative study, we have tried to identify these challenges and provide solutions to them. Methods: This is a qualitative content analysis study was performed with the incorporation of seven operating room and anesthesiology instructors of Bushehr University of Medical Sciences. The required questions were first collected and subsequently posed as interviews in the operating room setting. Data were collected through the recording of semi-structured interviews and until the data saturation. Results: Research findings are reported in three themes and nine categories. The main themes were organizational structure, planning, and human resources, each of which with specific categories and/or subcategories as per the subject matter. In terms of organizational structure, the most serious challenges comprised a lack of welfare facilities and problems in the field of education, such as non-specialization of operating rooms and limited physical space. The challenges related to planning comprised of a lack of student self-evaluation, delay in surgery initiation, and a large number of students in various fields. As for the human resource challenges, the most apparent problems were insufficient education delivered to students before their entrance to the operating room and the lack of familiarity of officials with the operating room environment. Conclusions: Given the questions posed to and the answers received from the instructors, most of the problems in the operating rooms in terms of education are related to the infrastructure-associated problems in the operating room and the studentschr('39') approach to the operating room. Despite the enormous efforts of the instructors in education, as they were respondents in the study, it could not be clarified how the problems in human resource and teacher dimensions could help solve the overall problems, suggesting that further research is required with the help of students and other groups involved in education in the operating room.
Skin Conductance Synchronization Among Family Members: A Systematic Review

Physiological synchronization has often been associated to empathy. Several investigations are present in psychotherapy research, which show a link between attachment and Skin Conductance Synchronization (SCS). Surprisingly, scarce number of investigations deepened the phenomenon of SCS in family research. Our first aim was to delineate the state of the art of SCS studies among family members. A systematic review has been carried out in accordance with PRISMA guidelines on Scopus and Web of Science online databases. Only eight studies were retrieved that applied SCS measures to parent-child dyads (infants and adolescents) and marital couples. As a second aim, interpretations and future possible applications – based on past psychotherapy research findings and present results – are discussed.
Efficient Electromagnetic Analysis of a Dispersive Head Model Due to Smart Glasses Embedded Antennas at Wi-Fi and 5G Frequencies

Numerical study of electromagnetic interaction between an adjacent antenna and a human head model requires long computation time and large computer memory. In this paper, two speeding up techniques for a dispersive algorithm based on finite-difference time-domain method are used to reduce the required computation time and computer memory. In order to evaluate the validity of these two speeding up techniques, specific absorption rate (SAR) and temperature rise distributions in a dispersive human head model due to radiation from an antenna integrated into a pair of smart glasses are investigated. The antenna integrated into the pair of smart glasses have wireless connectivity at 2.4 GHz and 5th generation (5G) cellular connectivity at 4.9 GHz. Two different positions for the antenna integrated into the frame are considered in this investigation. These techniques provide remarkable reduction in computation time and computer memory.
Wolman Disease with a Low Cholesterol Level: An Unusual Laboratory Finding

Wolman disease is a very rare autosomal recessive genetic disorder. The patients have the typical clinical finding of hepatosplenomegaly but with an abnormal lipid profile of high levels of total cholesterol (TC), triglycerides, and low-density lipoprotein cholesterol (LDL-C), but a low level of high-density lipoprotein cholesterol (HDL-C). We report a 1-month-old boy with Wolman disease who had hepatosplenomegaly but with an atypical abnormal lipid profile of low TC level, and very low levels of both LDL-C and HDL-C. The genetic study revealed a compound heterozygous mutation of the LIPA gene, leading to the confirmed diagnosis of Wolman disease.
Control of Pathogenic Vibrios in Shrimp Aquaculture using Antiinfectives from Marine Natural Products

Mid-culture outbreaks due to pathogenic Vibrio spp. are most common and frequent disease problems encountered in shrimp aquaculture. Literature showed that the decades old shell disease to recent early mortality syndrome are caused by vibrios as primary and/or secondary etiological agents. Poor water quality, deteriorated pond bottom due to over feeding, inadequate farm management, rapid intensification of stocking approaches etc. are major predisposing factors of disease outbreaks. Both reactive and proactive treatment methods are being progressed through R&D efforts, but tangible solution is remain to prevent/control midculture outbreaks in shrimp aquaculture. Alternate approaches to replace the use of antibiotics in aquaculture is a highly prioritized research area. Alternate approaches are being widened to explore marine natural products, smart biomolecules such as biosurfactants and poly-hydroxy butyrates (PHB), nanoparticles, quorum quenching molecules, small bioactive peptides and probiotics. The oceans are the single principal bio-resource of halo-metabolites produced by a range of marine organisms such as seaweeds, corals, sponges, molluscs, coelenterates, marine worms, tunicates, bacteria etc. but their utilization as aquaculture drugs are not being exploited. Application of seaweed secondary metabolites in treating shrimp bacterial diseases represents an easy, cost effective and environmentally benign venture for equitable and sustainable shrimp farming. Recently we reported antiadhesive activity of PHB biopolymer against Vibrio alginolyticus and V. harveyi, which were considered as the most significant pathogenic vibrios in the grow-out ponds of giant black tiger shrimp Penaeus monodon. In order to reduce the use of antibiotics, pesticides and other chemicals and to improve the ecological environment of shrimp farms, research is being focused on the potential use of marine probiotic bacteria in shrimp farms to improve water quality by balancing bacterial population in water and reducing pathogenic bacterial load.
Coffee Diterpenes: Quantification and Anti-angiogenic Effects

Coffee is one of the most important agricultural products in the world, from which a popular beverage is prepared for its particular aroma, taste and composition. The lipidic fraction of coffee is extremely complex and serves as carrier for flavors and for fat-soluble vitamins and contributes to texture and mouthfeel in the coffee brew. It is also regarded as an important source of bioactive components, such as diterpenes and diterpene esters that carry relevant nutritional or functional properties. Elevation of blood low density lipoprotein is the consequence of high diterpenes consumption. Nevertheless, degradation of toxic substances, anti-carcinogenic and anti-inflammatory properties of coffee diterpenes have been also documented. Moreover, they are used for authentication purpose in coffee industry. To contribute to a better understanding of the factors affecting the diterpenes level in coffee, this study aimed to provide information on the influence of species, blending, roasting, and brewing method on diterpenes content in coffee samples, as well as the anti-angiogenic effects of two diterpene esters. Firstly, the analytical methods for quantification of total diterpenes in coffee matrices (beans and brews) including cafestol, kahweol, 16-O-methylcafestol (16-OMC) and dehydrocafestol and diterpenes esters, including cafestol and kahweol linoleate, oleate, palmitate and stearate, were adapted from previous ones and conveniently validated, using high performance liquid chromatography combined with diode array detector (HPLC-DAD). Total diterpenes were analyzed after saponification of diterpene esters. The optimized conditions were: saponification at 80 °C of 2.5 mL of coffee brew or 200 mg of ground coffee with potassium hydroxide in methanol/water media, followed by two or four sequential extractions using diethyl ether in terms of coffee brew or ground coffee, respectively. The combined ether phase was cleaned with 5 mL of 2 M NaCl solution. The analytical methods proved to be linear (R2 ≥ 0.999) within the range of 0.3 300 mg/L and good precision (≤ 8%) and high recovery (81 – 102%) were achieved. Kahweol was the most important diterpene in Arabica brews, followed by cafestol, whereas, in Robusta brews, cafestol and 16-OMC were the main diterpenes. Cafestol, kahweol and 16OMC concentrations in coffee brews ranged between 0.3 – 140.1 mg/L , 0.3 190.6 mg/L, and
Risk Factors, Clinical Features and Treatment Outcomes of Male Breast Cancer in Saudi Arabia: A Single Center Study

The aim of this study was to determine the risk factors, clinical features and treatment outcomes of Male Breast Cancer (MBC) patients in Saudi Arabia. This retrospective-cohort study was conducted in Princess Nora Oncology Center at King Abdulaziz Medical City in Jeddah, Saudi Arabia. By using consecutive sampling, all male patients who were diagnosed with breast cancer based on the pathology results that were collected from the medical records from January 1998 to December 2017 and treated in Princess Nora Oncology Center at King Abdulaziz Medical City, Jeddah, Saudi Arabia were included in this study. Excel was used to encode all the patients’ data, while SPSS was used for the analysis. A descriptive statistic was used to find the minimum, maximum, and mean of age at the time of diagnosis. On the other hand, frequencies and percentages were utilized to summarize all other clinical features of the patients including the pathological type, stage at time of diagnosis, metastasis, risk factors, performance status, treatments and relapse. The mean age of all the 15 patients was 58 +/16.5 SD at the time of diagnosis, whereas the maximum was 91 and the minimum was 36. The most predominant type of male breast cancer was Invasive Ductal Carcinoma IDC, which affected 14 patients out of 15 (93.3%).
The Mirror of Malaysia Real Estate Investment Trust Stock Return in the Short-Run Dynamics

The formation of Islamic capital market under the subcomponent of Islamic financial system scratch a milestones development of Islamic finance in Malaysia. The Islamic capital market operates in mirror with convention capital market in expending, deepening and broadening Malaysia financial system. Malaysia is one of the REIT markets that value both the Islamic and conventional practices, such flexibility makes the attract not only to the local investor but also Islamic investors and foreign investor. The major source that generates income for REIT is the rental of the commercial real estate invested and hold as portfolio by the REIT management company. Furthermore, Malaysia REIT is known to be defensive stocks which consist of cyclic income producing assets that has some potential of asset appreciation. On the other hand, it witnessed by the moderation of Malaysia government bond yields created a lower pressure on the REIT stock price and analyst’s report highlighted the uncertainties on global crude oil prices and inflation is main concerned to REIT investors. In addition, the revision of 2019 tax system in Malaysia furnished a long run affected the dividend payout and volatility of REIT stock price. Therefore, this impact on the REIT stock liquidity and trading volume experiencing anil liquid trading. Therefore, the impact of external forces towards the mirror of two type of Malaysia REITs is significant to the investors, policy makers and government to outline the short-run relationship and facilitate future growth. The Vector auto regression model, granger causality and variance decomposition employed in this study to analyze the mirror of two types Malaysia REIT stock return. The empirical finding shows that the variability of dividend yield is vital explanatory variables to explain the both type of REIT stock return in Malaysia followed by interest rate for Islamic REIT stock return. The mirror of conventional REIT further implicated that trading volume and global crude oil price are useful to forecasting the changes in the stock return. Nutshell, this study provides a discussion of Malaysia REIT stock return behavior and it should be given necessary attention by researchers in ensuring the newly develop Islamic REIT are competitive and stability as the conventional REIT.
Synthesis and Bacterial Evaluation of The V(IV), Cr(III), Fe(III) and Co(II) Ions Complexes with Mixed Ligands (2-hydroxybenzaldine)-4- Aminoantipyrine and 8-Hydroxyquinoline

The primary ligand (2-hydroxybenzaldine)-4-aminoantipyrine (L)was prepared as achelating ligand, Which was treated with V(IV), Cr(III), Fe(III) and Co(II) ions in alcoholic medium in order to prepare series of new metal complexes in the presence of the Co-ligand 8-hydroxyquinoline. These complexes were prepared by convential method and characterized by available techniques, FT-IR, UV-Visible, magnetic susceptibility, flame atomic absorption technique as well as elemental analysis and conductivity measurements. From these studiesmeasurements, asquare pyramidal structure proposed for V(IV) while octahedral geometry for Cr(III), Fe(III) and Co(II) complexes. Thebacterial activity of the prepared complexes have been determine and compared with the ligands.
Effect of Using Densah Burs on Implant Stability and Peri-implant Marginal Bone Loss in Maxillary Implant Supported Partial Overdentures

Background: This study investigated the effect of Densah Burs on the primary and secondary implant stability, in addition toperiimplant mean marginal bone loss in maxillary implant supported partial overdentures.Materials and Methods: Ten patients with posteriorly edentulous maxillae following kennedy class I classification wereselected. Each patient received two implants; one in each side. On one side, the implant osteotomy was prepared usingconventional surgical drills while on the other side, Densah burs were used. Implant stability was measured at implant insertionand time of loading using Osstell device. Mean margial bone loss was measured at 6 and 12 month interval using Cone BeamCT scan.Results: Statistical Significant difference was noted between both types of drills on primary implant stability but not onsecondary implant stability or mean marginal bone loss at 6 and 12 month interval.Conclusion: Densah drilling Burs may improve initial implant stability; a situation that may help when immediate loading ofimplants is planned especially if they are splinted. Moreover, they might not have a different effect rather than conventionaldrilling burs on marginal bone loss.