Miracle-dz/UniFAR-FTUs huggingface dataset:
статьи МИКРОВЕЗИКУЛЫ ЕСТЕСТВЕННЫХ КИЛЛЕРОВ ЛИНИИ NK-92 ВЛИЯЮТ НА ФЕНОТИП И ФУНКЦИИ ЭНДОТЕЛИАЛЬНЫХ КЛЕТОК ЛИНИИ

Microvesicles (MVs) are small (100-1000 nm) subcellular structures produced by both motionless and activated cells that can transfer molecules to target cells, and regulate physiological and pathological processes. MVs of leukocyte origin, in particular those produced by natural killer cells (NK cells), remain the least studied population of MVs. NK cells can change the functional activity of endothelial cells (ECs) and are involved in regulating angiogenesis. The ability of NK cell-derived MVs to influence the functionality of ECs is understudied currently. We aimed to study the effect of MVs produced by NK cells of the NK-92 cell line on the phenotype, caspase activity, proliferation and migration of ECs of the EA.Hy926 cell line. We cultured ECs in the presence of MVs derived from the NK-92 cell line, and then used flow cytometry to assess changes in EC phenotype, intracellular protein transfer from MVs to ECs, and the relative death of ECs. We used western blot analysis to evaluate the expression of granzyme B in NK cells and in the MVs that they produced, as well as the expression of granzyme B, caspases, extracellular-regulated kinase ( ERK) and protein kinase B (AKT) in ECs. We also assessed the proliferation and migration of ECs in the presence of MVs derived from cells of the NK-92 cell line. The results revealed significant differences in the proteomic profiles of cells of the NK-92 cell line and their MV product. Contact between ECs and MVs derived from cells of the NK-92 cell line is accompanied by the following events: a) expression of granzyme B in ECs, b) activation of caspase-9 and caspase-3, with partial EC death, c) appearance of the panleukocyte marker CD45 on ECs, d) decrease in CD105 expression, and increase in CD34 and CD54 expression, and e) inhibition of EC migration. Transfer of ERK (but not AKT) from MVs derived from cells of the NK-92 cell line to ECs, at a concentration 10 times lower than that which causes EC death, leads to an increase in EC proliferation.
CALL FOR PAPERS Cell Signaling: Proteins, Pathways and Mechanisms Liver X receptor- (cid:2) and miR-130a-3p regulate expression of sphingosine 1-phosphate receptor 2 in human umbilical vein endothelial cells

receptor- and miR-130a-3p regulate expression of sphingosine 1-phosphate receptor 2 in human umbilical vein endothelial cells. Physiol Recent studies have shown that activation of liver X receptors (LXRs) attenuates the development of atherosclerosis, not only by regulating lipid metabolism but also by suppressing inﬂammatory signaling. Sphingosine 1-phosphate receptor 2 (S1PR2), an important inﬂammatory gene product, plays a role in the development of various inﬂammatory diseases. It was proposed that S1PR2 might be regulated by LXR- (cid:2) . In the present study, the effect of LXR- (cid:2) on tumor necrosis factor- (cid:2) (TNF- (cid:2) )-induced S1PR2 expression in human umbilical vein endothelial cells (HUVECs) was investigated and the underlying mechanism was explored. The results demonstrated that TNF- (cid:2) led to an increase in S1PR2 expression and triggered a downregulation of LXR- (cid:2) expression in HUVECs as well. Downregulation of LXR- (cid:2) with speciﬁc small interfering RNA (siRNA) remarkably enhanced the primary as well as TNF- (cid:2) -induced expression of S1PR2 in HUVECs. Activation of LXR- (cid:2) by agonist GW3965 inhibited both primary and TNF- (cid:2) -induced S1PR2 expression. GW3965 also attenuated S1PR2-induced endothelial barrier dysfunction. The data further showed that TNF- (cid:2) induced a signiﬁcant decrease in miR-130a-3p expression. Overexpression of miR-130a-3p with mimic product reduced S1PR2 protein expression, and inhibition of miR-130a-3p by speciﬁc inhibitor resulted in an increase in S1PR2 protein expression. Furthermore, activation of LXRs with agonist enhanced the expression of miR-130a-3p, and knockdown of LXR- (cid:2) by siRNA suppressed miR-130a-3p expression. These results suggest that LXR- (cid:2) might downregulate S1PR2 expression via miR-130a-3p in quiescent HUVECs. Stimulation of TNF- (cid:2) attenuates the activity of LXR- (cid:2) and results in enhanced S1PR2 expression.
Claw and leg disorders in cattle in relation to the design and construction of floors

In dit overzicht wordt het verband tussen de vloeruitvoering en klauw- en beenafwijkingen bij melkvee, vleesstieren en vleeskalveren beschreven. Ideeen voor verder onderzoek worden tevens naar voren gebracht
Series connected photovoltaic cells-modelling and analysis

As solar energy costs continue to drop, the number of large-scale deployment projects increases, and the need for different analysis models for photovoltaic (PV) modules in both academia and industry rises. This paper proposes a modified equivalent-circuit model for PV modules. A PV module comprises several series-connected PV cells, to generate more electrical power, where each PV cell has an internal shunt resistance. Our proposed model simplifies the standard one-diode equivalent-circuit (SEC) model by removing the shunt resistance and including its effect on the diode part of the circuit, while retaining the original model accuracy. Our proposed equivalent circuit, called here a modified SEC (MSEC), has less number of circuit elements. All of the PV cells are assumed operating under the same ambient conditions where they share the same electric voltage and current values. To ensure the simplification did not come at a reduction in the accuracy of the SEC model, we validate our MSEC model by simulating both under the same conditions, calculate, and compare their current/voltage (I/V) characteristics. Our results validate the accuracy of our model with the difference between the two models falling below 1%. Therefore, the proposed model can be adopted as an alternative representation of the equivalent circuit for PV cells and modules.
New Insights into the State Trapping of UV-Excited 2 Thymine 3

: After UV excitation, gas phase thymine returns to ground state in 5 to 7 ps, showing 18 multiple time constants. There is no consensus on the assignment of these processes, with a dispute 19 between models claiming that thymine is trapped either in the first (S 1 ) or in the second (S 2 ) excited 20 states. In the present study, nonadiabatic dynamics simulation of thymine is performed on the basis 21 of ADC(2) surfaces, to understand the role of dynamic electron correlation on the deactivation 22 pathways. The results show that trapping in S 2 is strongly reduced in comparison to previous 23 simulations considering only non-dynamic electron correlation on CASSCF surfaces. The reason for 24 the difference is traced back to the energetic cost for formation of a CO  bond in S 2 .
MONOTONICITY WITH RESPECT TO p OF THE FIRST NONTRIVIAL EIGENVALUE OF THE p -LAPLACIAN WITH HOMOGENEOUS NEUMANN BOUNDARY CONDITIONS

We deal with monotonicity with respect to p of the ﬁrst positive eigenvalue of the p -Laplace operator on Ω subject to the homogeneous Neu- mann boundary condition. For any ﬁxed integer D > 1 we show that there exists M ∈ [2 e − 1 , 2] such that for any open, bounded, convex domain Ω ⊂ R D with smooth boundary for which the diameter of Ω is less than or equal to M , the ﬁrst positive eigenvalue of the p -Laplace operator on Ω subject to the homogeneous Neumann boundary condition is an increasing function of p on (1 , ∞ ). Moreover, for each real number s > M there exists a sequence of open, bounded, convex domains { Ω n } n ⊂ R D with smooth boundaries for which the sequence of the diameters of Ω n converges to s , as n → ∞ , and for each n large enough the ﬁrst positive eigenvalue of the p -Laplace operator on Ω n subject to the homogeneous Neumann boundary condition is not a monotone function of p on (1 , ∞ ).
Diagnostic Value of Upper Lip Bite Test with Hyomental Distance Ratio in Assessing the Airway

Introduction: Airway assessment before any type of anesthesia is mandatory. Upper lip bite test (ULBT) is a relatively new test for assessing the airway. Range of freedom of mandible and dental architecture has a significant role in facilitating intubation, both of which are assessed by this single test. ULBT with good neck movements are sufficient criteria for assessing the airway. Hyomental distance ratio (HMDR) assesses the range of neck movements. So by combining these two tests we expect an increase in reliability and validity. the modified Cormack and Lehane Difficult Grade 3 or 4 class 3, HMDR with optimal cutoff point difficult intubation predictors. Sensitivity, Specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV) and Accuracy of both ULBT and HMDR separately and then both in combination are calculated and compared with those of MP. Results: ULBT:-Sensitivity - 88.2%, Specificity - 99.62%, PPV - 96.77%, NPV - 98.51%, Accuracy - 98.33% HMDR: - Sensitivity - 88.2%, Specificity – 99.25%, PPV - 93.75%, NPV - 98.50%, Accuracy - 98.00% MP: - Sensitivity - 17.65%, specificity - 88.72%, PPV - 16.67%, NPV - 89.39%, Accuracy - 80.66% ULBT+HMDR: - Sensitivity - 82.35%, specificity-100%, PPV100%, NPV97.75%, Accuracy - 98.00 % Conclusion: Upper Lip Bite test is good screening test for assessing the airway. ULBT in combination with HMDR increased the specificity and positive predictive value.
Clinicopathological Profile of Chronic Myelomonocytic Leukemia Cases

Background: Chronic myelomonocytic leukemia (CMML) is a clonal hematological neoplasm with features of both myeloproliferation and myelodysplasia with an incidence 0.4 per lakh population. A number of prognostic risks scoring systems have attempted to predict survival and risk of CMML patients, like International Prognostic Scoring System (IPSS), the Spanish Score, the modified Bournemouth Score, the Dusseldorf Score and the MDAP Score. But no prognostic system has been widely accepted. More data from different regions are required to create a widely accepted working prognostic system for CMML. No case series on CMML has been published in literature from India in our knowledge. This article attempts to put to light the various clinicopathological parameters of CMML cases from India and the impact of these parameters on final outcome. Methods: All admitted patients in a tertiary center in western India, with a diagnosis of either a chronic myeloproliferative disease or a myelodysplastic disease over a period of 3 years (2015-2018) were evaluated, out of which nine(n=9) cases fulfilled the diagnostic criteria of CMML. All patients underwent peripheral blood examination, bone marrow aspirate, bone marrow biopsy and cytogenetic studies. Result: All patients (n=9) were between 50 and 80 years and most were males (n=8). Five patients presented with hepatosplenomegaly. Renal liver function of one patient was deranged who had pleural effusion, ascites. Most patients (n=8) had total leukocyte count above 13000/cumm, while three had low platelet counts. Two out of three patients classified as CMML-II with increased blasts in peripheral blood and bone marrow had fatal outcomes. Patients whose karyotypes were available had normal karyotypes without any additional cytogenetic abnormalities. All were negative for JAK2 and BCR-ABL1. Conclusion: The study concluded that altered biochemical tests (LDH, LFT), blast percentage, CMML II, relative lymphocytosis and transformation to AML were associated with poor outcome.
An investigation of adhesion performance under various conditions using heat-treated wood

In this study, the adhesion performance of oak (Quercus petrea Liebl.) And Scotch pine (Pinus Silvestris L.) wood samples that were heat treated and glued with 1K-PUR adhesive were kept under different conditions (A1-A4-A5) and moisture contents (20°C /% 35, 20°C /%65 ve 20°C /% 95), and then the adhesion performance was investigated. Rough sized pieces of both types of wood were heat treated at 195°C for 2 hours. Test samples were prepared from untreated and heat treated blanks (DIN EN 204). Tensile tests according to DIN EN 204 were applied to samples kept at 20°C/35%, 20°C/65% and 20°C/95% temperature and relative humidity, respectively, and the adhesion performance was determined in three climatic conditions. At the end of these tests, it was found that the holding environment and climatic conditions have a significant effect on the adhesion resistance. It was observed that the samples that were kept under A1 conditions and tested had wood rupture (error) between 70% and 100%, and the samples tested after holding in A4 and A5 conditions mostly broke off from the glue joint.
Some Economie Interprétations of Migration

For many years économie development was considered as a process of graduai change in thé structure of thé economy i.e. in thé changing significance of different economie sectors. Increasing productivity, and therefore production, in agriculture induced a shift of labour towards thé more industrial and urban sectors. In thé latter productivity increased even more rapidly as a resuit of appîied technological innovations in production processes and a greater spécialisation in thé division of labour tasks. Increasing incomes allowed for more capital accumulation, which in turn paved the way for further expansion of industry and agriculture. Forward and backward linkages in différent industrial activities and thé expansion of skilled labour in numerous branches were other growth inducing factors. Industrial sectors showed far higher growth rates in comparison to agriculture and thus were considered thé prime movers of growth. At a later stage and a higher level of ongoing income growth and capital formation, thé service sector (banks, transport, trade, communication etc.) gained importance and allowed for an ever expanding complex of commercial, industrial and agricultural interdependences. During this process a shift in labour absorption in thé three main sectors (agriculture, industry and services) took place. Labour utilisation in agriculture was gradually diminished in favour of the other two sectors. And in actual fact among ail thé industrialized countries agriculture as a source of labour absorption has little significance, and absorbs far less than 10% of the total labour force. Industry and thé service sector have since long taken over the rôle of large sectors of labour utilisation.
Classification Amenorrhea of Hypothalamic Progestin-Nonresponsive by Secretion Pattern of Serum LH

The pathogenesis of hypothalamic progestin-nonresponsive amenorrhea is unclear and this disease often fails to respond to treatment. The pulsatile patterns of diurnal and nocturnal secretion of serum LH as well as serum levels of melatonin were examined to improve the understanding of the pathogenesis and to develop strategies for the management of a severe type of hypothalamic amenorrhea. Four types of LH pulsatile patterns were observed: a) no pulse during the day or night (Group 1); b) more than 1 pulse only at night (Group 2); c) only 1 pulse during the day and more than 2 pulses at night (Group 3); and d) more than 2 pulses during the day and at night (Group 4). Serum estradiol was less than 30 pg/mL, and the serum PRL and PRL response to TRH did not differ among the four groups. The basal level and the pulse amplitude of LH increased successively from Group 1 to Group 4. The serum level of melatonin at night was noticeably increased in Group 1 and correlated negatively with the LH pulse frequency at night. After 6-month hormone replacement therapy with estrogen and progesterone, the rate of improvement in ovarian function were 0%, 33.3%, 57.1% and 67.0% in Groups 1, 2, 3, and 4, respectively. In 5 patients, the LH pulse pattern was re-examined at 6 months, the LH pulsatile pattern was changed from that of Group 1 to that of Group 4, with a decrease in serum concentrations of nocturnal melatonin, indicating improved ovarian function. In conclusion, classification of patients according to the LH secretion pattern is useful in establishing the severity of hypothalamic disturbance in hypothalamic progestin-nonresponsive amenorrhea and in predicting its prognosis; in addition nocturnal melatonin can be used as a marker for severer cases of hypothalamic amenorrhea.
Comparison of CFD simulations with experimental Jet Erosion Tests results

: The Jet Erosion Test (JET) is an experimental device increasingly used to quantify the resistance of soils to erosion. This resistance is characterised by two geotechnical parameters: the critical shear stress and the erosion coefficient. The JET interpretation model of Hanson and Cook (2004) provides an estimation of these erosion parameters. But Hanson’s model is simplified, semi-empirical and several assumed hypotheses can be discussed. Our aim is to determine the relevance of the JET interpretation model. Therefore, we developed a numerical model able to predict the erosion of a cohesive soil by a turbulent flow. Our numerical model is first validated on a benchmark: erosion of an erodible pipe by a laminar flow. The numerical results are satisfactorily compared with the theoretical solution. Then, three JETs are modelled numerically, with values of erosion parameters obtained experimentally. A parametric study is also conducted to validate the accuracy of the numerical results and a good agreement is observed. The erosion parameters found experimentally permit to predict numerically the evolution of the erosion pattern within good accuracy. This result contributes to the validation of the JET’s semi-empirical model. The numerical model also gives a complete description of the flow, including vortices which can be observed in the cavity created by erosion. The whole erosion pattern evolution is given by the numerical results. Our numerical model gives information that is not available otherwise. by the hatched zone between the two curves. The experimental results were obtained by direct
Two novel and potentially endemic species of Phytophthora associated with episodic dieback of Kwongan vegetation in the south-west of Western Australia

Two novel homothallic species of Phytophthora causing dieback of Kwongan vegetation in south-west Western Australia are described here as Phytophthora arenaria sp. nov. and Phytophthora constricta sp. nov. DNA sequencing of the ITS rDNA and cox 1 gene conﬁrmed that P. arenaria and P. constricta are unique species residing in ITS clades 4 and 9, respec-tively. Phytophthora arenaria has been isolated from vegetation occurring on the northern sandplains which are warmer and drier than the southern sandplains from which P. constricta has been predominantly isolated, and both species appear morphologically and physiologically well adapted to the ecosystems in which they occur. Both species have been associated mainly with dead and dying Banksia species and the pathogenicity of both P. arenaria and P. constricta to Banksia attenuata was conﬁrmed in this study. The combination of unique DNA sequences, including considerable variation in cox 1 sequence data, thick oospore walls and physiological characteristics that appear to be adaptations favouring survival in the harsh Kwongan ecosystem suggest that these species may be endemic to Western Australia.
Shareholder wealth effects of M&A withdrawals

This paper provides evidence on the wealth effect in the event of the withdrawal of a merger or acquisition, and the impact of termination fee provisions on acquirer withdrawal returns. I report a signiﬁcant negative correlation between acquirer withdrawal returns and announcement returns, consistent with the theory of managerial learning in M&As. Target ﬁrms reap net gains in deal withdrawals, showing evidence of a permanent revaluation of targets even if the deals fail. I also ﬁnd that acquirer termination fee provisions are positively associated with acquirer withdrawal returns, suggesting that such provisions may play a disciplinary role in the withdrawal decision-making and protect acquirer shareholders’ interests in deal withdrawals. Furthermore, my results also show that target termination fee provisions are negatively associated with acquirer withdrawal returns, which supports the efﬁciency hypothesis.
A GLP‑1 receptor agonist attenuates human islet amyloid polypeptide‑induced autophagy and apoptosis in MIN6 cells

Type 2 diabetes mellitus (T2DM) is characterized by the dysfunction and loss of pancreatic islet β -cells, in part due to islet amyloid deposits derived from islet amyloid polypeptide (IAPP). The glucagon-like peptide-1 (GLP-1) receptor agonist exendin-4 enhances the insulin secretory response by increasing β -cell mass in T2DM. However, it is unknown whether exendin-4 protects β -cells from IAPP-mediated autophagy and apoptosis. In the present study, reverse transcription-quantitative polymerase chain reaction, ELISA and western blotting were used to detected the mRNA and protein expression of insulin/hIAPP and other signaling molecules, while the mechanisms underlying these effects were also determined. Exendin-4 increased the level of insulin secretion, which was greater than that of IAPP, leading to a beneficial IAPP/insulin secretion pattern. In MIN6 cells incubated with 25 mM glucose, exendin-4 decreased the ratio of light chain 3 (LC3)-II/I, which was accompanied by an increase in p62 protein. In a hIAPP-overexpressing MIN6 cell model, exendin-4 prevented the hIAPP-induced increase in the LC3II/I ratio and decrease in p62 expression. In addition, exendin-4 pretreatment reduced hIAPP-induced activation of cleaved caspase-3, suggesting that exendin-4 may protect MIN6 cells against apoptosis. Taken together, the results highlight hIAPP as a critical mediator of β -cell loss and suggest that the GLP-1 receptor agonist exendin-4 may be a potential therapeutic agent for hIAPP-induced β -cell damage.
The Prevalence of Pruritus among Sudanese Patients with Diabetes Mellitus

Background: Diabetes mellitus is a chronic disease caused by inherited and/or acquired deficiency in the production of insulin by the pancreas, or by the ineffectiveness of the insulin produced. The skin is one of the major organ systems involved in diabetes. Almost all diabetic patients eventually develop skin complications from the long-term effects of diabetes mellitus on the microcirculation and skin collagen. Objectives: This study was aimed to detect the clinicoepidemiological prevalence of pruritus in diabetic patients on Jabir Abu Eliz Diabetic Centre. Methodology: It was observational, descriptive, cross-sectional and diabetic center-based study was conducted in the period from August to December 2014 at Jabir Abueliz Diabetes Centre (JADC), including 100 known diabetic patients (Had old and new files), from both sexes who were complaining of itching. The data were collected from patient’s records by questionnaire and analyzed by computer using SPSS. Results: The results of this study showed that diabetic patients who were complaining of on Jabir Abu Eliz Diabetic Centre was 23.3% (100 patients), found to be 42.0% of them were males and 58.0% were females. The clinical features of pruritus in diabetic patients in this study were an eruption, dark pigmentation, and dryness (29%), 3%, and 7%) respectively. Localized pruritus that attributed to diabetes was the most common among the study population.