Miracle-dz/UniFAR-FTUs huggingface dataset:
Analytical and Preparative Separation Methods of Biomacromolecules

Analysis of HbA and some Hb variants by HPLC analysis of posttranslational modifications in recombinant proteins by HPLC and mass spectrometry analytical and preparative separations of peptides by capillary and free-flow zone electrophoresis bioaffinity chromatography characterization and partial purification of steroidongenic factors from thymic epithelial cell-conditioned medium determination of affinity constants of lectins for sugars by affinity capillary electrophoresis displacement chromatography of biomolecules high performance membrane chromatography of proteins HPLC purification of recombiant proteins HPLC purification of recombiant proteins isolation, purification, and characterization of human seminal plasma proteins and their immunological behaviour in vitro isolation, purification, and structural study of allergenic proteins multiangle light scattering combined with HPLC with examples for biopolymers purification and characterization of connective tissue growth factor using heparin affinity chromatography slalom chromatography - a new hydrodynamic-based chromatographic mode applicable to size-dependent separation and physiochemical analysis of large DNA molecules simultaneous chromatographic separation of ceruloplasmin and serum amine oxidase fast, single-step affinity chromatography purification of haemoglobin.
Experimental Study and Modeling of Freeze-Drying in Syringe Configuration. Part II: Mass and Heat Transfer Parameters and Sublimation End-Points

This work concerns the experimental study in syringe configuration of the sublimation period for a model formulation under operating conditions commonly used for pharmaceutical proteins freeze-drying. Different methods for the detection of the sublimation end-point were compared. This exhaustive comparison has shown that the Lyotrack sensor (Annecy, France) had the best sensitivity and presented the best signal-to-noise ratio followed by the Pirani gauge; a significant advantage of Lyotrack sensor relies on the possibility of sterilization. The scale method, which is based on continuous weighing of a single syringe or a single vial, underestimated largely the sublimation times and, consequently, was not representative at all of the whole syringe (or vial) set behavior. Consequently, caution should be taken for its use for setting up laboratory data and for scaling up these data for the optimization of commercial freeze-drying cycles. Moreover, four standard freeze-drying cycles with different values of main operating parameters (shelf temperature and total gas pressure) were investigated in order to determine overall heat and mass transfer coefficient values in syringe configuration. As expected, higher shelf temperature resulted in higher sublimation rates, but higher total gas pressures led to lower sublimation rates; nevertheless, the total gas pressure influence was less pronounced than the shelf temperature influence. The influence of syringe location in the supporting rack on drying rate values was also investigated: no front/back location influence was observed, but a small side location effect was observed, as syringes located at the edge and at the center of the rack presented slightly higher mean sublimation rates. The two main sublimation parameters, namely, the water vapor mass transfer resistance, noted Rp, and the overall heat transfer coefficient, noted Kv, were determined by the pressure rise analysis method (PRA method) by using on-line and in situ pressure rise tests. The Kv values were higher when the gas temperature or the total gas pressure increased but the Rp values remained quite constant as a function of these two parameters. A comparison between syringe and vial configuration data proved that when glass vials were used instead of syringes, the values of the sublimation rates and of the permeability of the freeze-dried matrix did not change significantly.
The flocculation of kaolin suspensions with anionic polyacrylamides of varying molar mass and anionic character, 2. Polarographic study of the adsorption of anionic polyacrylamides onto clay†

A selective method using polarography was developed for the determination of the degree of adsorption of anionic polyacrylamides onto Kaolinite. Four polymers of the same anionic character but different molar mass were studied. Limiting adsorption of the polyacrylamides onto the clay increased with their molar mass.
Transient Heat Transfer Characteristics Between Molten Fuel and Steel with Steel Boiling in the CABRI-TPA2 Test

Abstract In the TPA2 test of the CABRI-RAFT program, which is part of a fast reactor safety study, fuel-to-steel heat transfer characteristics within a molten fuel/steel mixture system have been investigated. This test was performed in the French CABRI reactor and used a test capsule that contained fresh 12.3%-enriched UO2 pellets with embedded stainless steel balls. Following a preheating phase, the capsule was subjected to a transient overpower that resulted in fuel melting and steel vaporization. The observed steel vapor pressure buildup was quite low, which suggested the presence of a mechanism that significantly reduced the fuel-to-steel heat transfer. A detailed experimental data evaluation by SIMMER-III led to one possible interpretation that the steel vaporization at the surface of the steel ball blanketed the steel from the molten fuel.
Inverse Size Exclusion Chromatography (ISEC) for Determining the Relative Pore Size Distribution of Wood Pulps

A method for preparing chromatographic columns containing virgin (never-dried) or previously dried wood pulp fibres for inverse size exclusion chromatography (ISEC) has been developed. The method was optimised with regard to packing density, pump speed of the eluation solution and probe molecule concentration using a multi-factor design in order to obtain the best and the most accurate resolution with regard to the apparent pore size distribution for the never-dried wood pulps. The method is based on the packing of a fibre bed of intact fibres through which sugar standards of different molecular weight are passed. The pulps are fractionated to remove all fines and converted to their sodium form as a standard procedure before the column packing procedure. The packing was performed by filtering a fibre suspension through a growing column of packed pulp fibres. The total pore volume was determined to be 1.1 and 1.2 ml/g for never-dried bleached and unbleached sulphate pulps respectively using the optimised parameters. The results indicate a good reproducibility for the method.
Calibrating the BKW-EOS with a large product species data base and measured C-J properties

The Becker-Kistiakowsky-Wilson equation of state (BKW-EOS) has been calibrated over a wide initial density range near C-J states using measured detonation velocities, pressures and temperatures from 62 explosives at 111 total initial densities. Values for the empirical BKW constants [alpha], [beta], [kappa], and [theta], were 0.5, 0.298, 10.5, and 6620, respectively. Values for the co-volume factors k[sub i], were assumed to be invariant. Model evaluation includes comparison to measured detonation properties from 91 explosives composed of combinations of Al, B, Ba, C, Ca, Cl, F, H, N, O, P, Pb, and Si at 147 total initial densities. Adequate agreement between predictions and measurements were obtained with a few exceptions. The exceptions were explosives which contained large amounts of aluminum, ammonium nitrate, barium nitrate, and silicon, as well as tetranitromethane and lead styphnate. However, detonation properties for the aluminum and nitrate containing explosives can be predicted adequately by assuming partial equilibrium. The performance of explosives containing large amounts of silicon can be predicted adequately by changing [beta] from 0.298 to 0.249. Performance of tetranitromethane and lead styphnate can also be predicted by adjusting [beta] and/or [kappa] slightly. 5 tabs, 3 figs, 19 refs.
Approaches to Support Ventricular Assist Device Patients in the Community : a Scoping Review

iii Lay Summary . v Preface . vi List of Tables . x List of Figures . xi Chapter 1: Introduction . 1 Background . 1 Ventricular Assist Devices . 2 Potential Complications . 4 Outpatient Surveillance . 6 The Challenges of Outpatient Care . 8 Support Approaches . 10 Benefits of Community Management . 10 Rationale . 11 Research Questions . 12 Conceptual Framework . 12 Study Significance . 14 Chapter 2: Methods . 15 Scoping Review . 15 Stage I: Identifying the Research Question . 16
Functional Properties of Cassava Tapioca Grits

This study investigated the functional properties of tapioca grits produced from wet starches from 39 different cassava varieties (36 cassava mosaic disease resistant varieties CMD clones and 3 checks TMS 30572, 4(2) 1425 and 82/00058). There were significant differences (p < 0.05) for functional properties of tapioca grits from different cassava varieties. Dispersibility of tapioca ranged from 6–29%, water absorption capacity (WAC) ranged from 415.13–595.26%, swelling power ranged from 20.76–26.92%, solubility index ranged from 4.04–20.42%, color intensity ranged from 87.79–92.09%. Granule size of pre-gelatinized tapioca ranged from 12.50–22.50 μm. Significant differences (p < 0.05) were obtained on the effect of cassava varieties, viscometer speeds and temperature of tapioca meal. The viscosity of the tapioca meal decreased (37.90–0.72 Pa.s) with increasing shear rates at both 30 and 40°C, but higher for lower temperature respectively. Tapioca meal produced from cassava variety M98/0028 had the highest viscosity 37.90 Pa.s at 13.66 (s-1).
Handbook on metals in clinical and analytical chemistry

General aspects of the role of metals in clinical chemistry collection, transport, and storage of biological samples for the determination of trace metals method evaluation, quality control and external quality assurance systems of analytical procedures spectrophotometry atomic absorption spectrometry ion-selective electrodes - an overview pH measurements and other applications voltammetry ion chromatography analysis by gas chromatography-mass spectrometry inductively coupled plasma-atomic emission spectrometry (ICP-AES) and inductively coupled plasma-mass spectrometry (ICP-MS) neutron activation analysis and y-spectrometry solid sampling analysis determination of metals in human hair aluminum antimony arsenic barium beryllium bismuth cadmium calcium cesium chromium cobalt copper europium and other lanthanides gadolinium gallium germanium gold indium iron lead lithium magnesium manganese mercury (part contents)
Characteristic Aroma Components of the Volatile Oil of Yellow Keaw Mango Fruits Determined by Limited Odor Unit Method

Odor detection thresholds of optically active compounds and other volatile compounds found in the oil of yellow Keaw mangoes were determined. Odor intensity of individual components was evaluated by Lod (limited odor unit) based on data of the odor detection threshold and the concentration of individual components at the recognition threshold level of the volatile oils. � -Damascenone and terpinolene were found to have Lod values greater than one and were identified as the components most responsible for the characteristic aroma. Odor recognition threshold of a mixture of 15 chemicals having larger Lod values against natural Keaw mango oils was 1.8 ppm, which exceeded that of Keaw mango oils (0.62 ppm) against Ok-rong mango oils. The mixture of fifteen compounds comprising � -damascenone, terpinolene, ethyl hexanoate, (E,Z)-(2,6)-nonadienal, 2,5-dimethyl-4-methoxy-3(2H)-furanone, (3R)-(� )-linalool, ethyl butyrate, ethyl octanoate, ethanol, (1S)-(� )-� -3-carene, (1S,5S)-(� )-� -pinene, trans-linalool oxide, (3S)-(� )linalool, butyric acid, and p-methylacetophenone was judged to possess an aroma very similar to that of the natural Keaw mango. Thus, these fifteen compounds were the key contributors to the aroma of Keaw mango.
Effect of cross-sectional change of a board specimen on stress wave velocity determination

Non-destructive evaluation (NDE) techniques employing longitudinal stress waves have been used in a variety of applications in the forest products industry. They have been shown to be very efficient methods in the assessment of log characterization (Bucur 1995). Basically, these evaluations estimate the velocity of the longitudinal stress waves in logs based on a vibration approach and thus determine the quality of the piece. The accuracy of the process is high. An important question should be taken into account: the theory is based on a uniform cross-sectional area along the length of the specimen. In the case of log specimens the cross-sectional area of the log is different at the two ends. This question was studied in detail for the flexural vibration of logs (Sobue 1990). The purpose of the research described in this short note was to investigate the effect of cross-section changes in longitudinal vibration for various area ratios. The area ratio (Ar) is defined as the ratio of the larger to the smaller cross-sectional area. The effect of cross-section changes on the stress wave velocity may be assessed in two ways: using finite element analysis or through experimentation. We chose the second approach, and carried out a large number of tests to assess this phenomenon. When using longitudinal vibrations, the following equation is used for stress-wave velocity determination:
HEALTH BELIEFS OF WOMEN SUFFERING FROM CANCER: A HOSPITAL BASED STUDY

Background- Cervix cancer and breast cancer are common cancers in Indian females. Many of the patients areilliterate and are often from a rural habitation and background. Patients come from diverse socioeconomic andsocio-cultural background. Many a times, these patients have different beliefs with regard to the causation, treatment and cure of disease. Objectives- The main objective of the study is to examine and provide an insight intothe health beliefs of women with cervical and breast cancer and its association with the socioeconomic- demographic profile of the patients. Methodology- This cross sectional study was carried out on 700 newlydiagnosed breast and cervix cancer patients attending the S.S. Hospital, BHU. The patients were interviewed using a pre tested and structured questionnaire. Result- Mean age of respondents was 50.35 + 9.86, 93.4% were Hinduby religion, 59.7% of the patients were illiterate and 87.7% were housewives. Patients of OBC caste constituted43.9% of total, 77.9% belonged to rural areas and only (12.3%) patients were of high socio-economic status.Approximately 30% of the patients believe that cancer is due to spiritual cause, i.e. origination from god and goddesses. Conclusion- The results indicate that a number of misconceptions in relation to causation of cancerexists in this population. The findings reveal that personal factors and supernatural causes were more stronglyrepresented in the belief system of patients.
Curcumin-loaded poly(l-lactide-co-D,l-lactide) electrospun fibers: Preparation and antioxidant, anticoagulant, and antibacterial properties

Fibrous materials of poly(l-lactide-co-d,l-lactide), poly(l-lactide-co-d,l-lactide)/poly(ethylene glycol), and curcumin were prepared by electrospinning. The incorporation of poly(ethylene glycol) in the fibers caused a decrease in the mean fiber diameters down to 700 nm and in the water contact angle value, the latter being equal to 0 at poly(l-lactide-co-d,l-lactide)/poly(ethylene glycol) 60/40 weight ratio. The water contact angle values of poly(l-lactide-co-d,l-lactide)/curcumin fibrous materials considerably exceeded those of films of the same composition (approximately 120° as compared to approximately 95°, for electrospun mats and solution-cast films, respectively). Curcumin affected the thermal stability of the fibrous materials and the crystallinity degree of the polymers. The mechanical properties of the electrospun materials also depended on the composition of the polymer matrix and the amount of curcumin therein. In the curcumin-containing fibers, curcumin was found in the amorphous state, while higher antioxidant activity was exhibited by poly(l-lactide-co-d,l-lactide)/poly(ethylene glycol)/curcumin fibrous materials. In vitro determination of the activated partial thromboplastin time and prothrombin time showed that poly(l-lactide-co-d,l-lactide)/curcumin fibrous materials displayed anticoagulant activity. Antibacterial effect toward Staphylococcus aureus was manifested by the curcumin-containing mats.
Distribution of Vibrational Energy Levels of Protein Molecular Chains

The distributions of the quantum vibrational energy levels of the protein molecular chain are found by the discretely nonlinear Schrodinger equation appropriate to protein obtained from the Davydov theory. The results calculated by this method are basically consistent with the experimental values. Furthermore, the energy spectra at high excited states have also been obtained for the molecular chain which is helpful in researching the properties of infrared absorption and Raman scattering of the protein molecules.
Orbital Interactions in Chemistry

Atomic and Molecular Orbitals. Concepts of Bonding and Orbital Interaction. Perturbational Molecular Orbital Theory. Symmetry Considerations. Molecular Orbital Construction from Fragment Orbitals. Molecular Orbitals of Diatomic Molecules and Electronegativity Perturbation. Molecular Orbitals and Geometrical Perturbations. State Wavefunctions and State Energies. Molecular Orbitals of Small Building Blocks. Molecules with Two Heavy Atoms. Orbital Interactions Through Space and Through Bonds. Polyenes and Conjugated Systems. Solids. Hypervalent Molecules. Transition Metal Complexes: A Starting Point at the Octahedron. Square Planar, Tetrahedral ML4 Complexes and Electron Counting. Five Coordination. The C2v ML3 Fragment. The ML2 and ML4 Fragments. Complexes of ML3, MCp and Cp2? M. The Isolobal Analogy. Cluster Compounds.
Fission product behavior in the Molten Salt Reactor Experiment

Essentially all the fission product data for numerous and varied samples taken during operation of the Molten Salt Reactor Experiment or as part of the examination of specimens removed after particular phases of operation are reported, together with the appropriate inventory or other basis of comparison, and relevant reactor parameters and conditions. Fission product behavior fell into distinct chemical groups. Evidence for fission product behavior during operation over a period of 26 months with $sup 235$U fuel (more than 9000 effective full-power hours) was consistent with behavior during operation using $sup 233$U fuel over a period of about 15 months (more than 5100 effective full- power hours). (auth)
Metal Cation-Supported Supramolecular Crown Ethers Featuring Hydrogen-Bonded Tetrameric Unit of 2-Hydroxy Pyridines

Three solid compounds formulated as [Cu(ClpyOH)4(H2O)2](PF6)2·(ClpyOH)4 1, [Co(pyOH)4(H2O)2](ClO4)2 2, and [Fe(ClpyOH)4Br2] 3 (ClpyOH = 5-chloro-2-hydroxy pyridine; pyOH = 2-hydroxy pyridine) have been shown by single-crystal structural analysis to be 3D networks, which are constructed by metal cation-supported crown ether-like species featuring hydrogen-bonded tetrameric unit of 2-hydroxy pyridines through extensive intermolecular interactions. The similar 16-membered hydrogen-bonded macrocyclic rings are still remaining in their solid structures when the molar ratio of M(II) and ligand were changed to 1:2 or 1:3 in their reaction systems for 1−3, suggesting that this kind of macrocyclic ring is more stable than other products of M(II) and ligand. Differences in the overall structural architectures in 1−3 are due to the interplay between the strong O−H···O hydrogen-bonding interactions and/or a variety of weaker interactions involving the halogens, C−H donors, and the aromatic systems.
Synthesis and Characterisation of Barium Strontium Cobalt Iron Oxide Mixed Ionic and Electronic Conductors

Oxygen separation membrane technology is an alternative to cryogenic distillation for the production of oxygen. Barium strontium cobalt iron oxide (BSCF, Ba0.5Sr0.5Co0.8Fe0.2O3-d) is one of the candidate membrane materials for this application. A robust wet-chemical synthetic route for the synthesis of BSCF has been developed. The route comprises reverse precipitation from a mixed metal nitrate solution at pH> 10 and uses a minimum amount of reagents. In particular there are no organic additives which need to be burnt off. All the metal ions precipitated simultaneously but a single phase was not then formed. The phases formed during precipitation, drying and calcining varied with the experimental conditions used. It was shown that ~80% of the barium and strontium precipitated as their mixed carbonate. However, on sintering, a single cubic phase material was formed. The process was capable of variation in the BSCF composition and the addition of copper to the system. The electrical and oxygen permeation properties were measured. The relationship between them was found to be dependent on the lattice parameter, tolerance factor and oxygen non-stoichiometry (d). Values of d greater than 0.5 at high temperature were found to give poor oxygen permeation and an increase in resistance although they did not decompose to the ordered brownmillerite structure. The addition of copper to BSCF by substituting it for iron and cobalt enhanced grain growth during sintering. However, except at the lowest addition level of 0.05 on the B-site, it did not show any oxygen permeation. High temperature x-ray diffraction (HTXRD) and x-ray photoelectron spectroscopy (XPS) studies were carried out. They showed that the thermal expansion coefficient increased at ~400� as loss of oxygen from the material started. As the materials were heated in the XPS surface carbonates were removed and the surface concentration of Ba and Sr ions decreased at 3-500�C although on further heating to 800�C their concentration returned to close to the original values.
Synthesis and photocatalytic oxidation properties of iron doped titanium dioxide nanosemiconductor particles

The structure, physical characteristics and selective photocatalytic oxidation properties of quantum confined nanosize iron doped TiO2 (Q-TiO2/Fe3+) particles were studied by TG-DSC, XRD, DRS, EPR and Selective Oxidation Photocatalytic Measurements. It is shown that the solubility of iron in the obtained Q-TiO2/Fe3+ nanoparticles is 1.0 atom% and the iron doping level has a great influence on the transformation of anatase to rutile (A-R). The quantum confined effect was observed for Q-TiO2/Fe3+ nanoparticles. All of the samples have EPR Bulk-Fe3+ and Surf-Fe3+ signals, which are located in the bulk and surface of TiO2 nanoparticles respectively. Quantitative EPR results indicate that the relative EPR intensity of these paramagnetic centers shows regular change with varying corresponding iron modification level. In situ EPR indicates that the photo-generated charge carrier (h+, e−) could be trapped by different Fe3+ sites simultaneously, i.e., trapping of h+ is due to Surf-Fe3+ sites at g = 4.30, whereas that of e− is attributed to Bulk-Fe3+ sites at g = 1.99. Selective photocatalytic oxidation of cyclohexane into cyclohexanol with higher selectivity has been obtained by molecular oxygen activation over Q-TiO2/Fe3+ nanoparticles under mild conditions. It is thought that the optical surface state of Q-TiO2/Fe3+ nanoparticles play a key role in the selective photocatalytic oxidations.