Miracle-dz/UniFAR-FTUs huggingface dataset:
Lung adenocarcinoma radiosensitivity depends on EGFR signaling pathway or other pathways? —— a research based on gene sequencing and data-independent acquisition (DIA) mass spectrometry

Background: To investigate the biological basis of radiosensitivity difference in lung adenocarcinoma cells, EGFR mutant or a wide variety of signal pathways？ ﻿Methods: There were fourteen lung adenocarcinoma cell lines used to carry out experiments in this study. The SF2 experimental test data of fourteen cell lines used in this study are from the previous work we did. EGFR mutation in each cell line detected by Next-Generation Sequencing. Statistical difference in SF2 about fourteen cells used the Kruskal-Wallis test. Correlation between EGFR mutant or not and SF2 analyzed by Student’s t-test. The proteomes of the 14 lung adenocarcinoma organisms were identified by DIA in LC-MS/MS proteomic technology. P < 0.05 showed statistical significance. R language software was used to perform the bioinformatics analysis.Results: The SF2(Surviving fractional 2Gy ) median value is 0.351(0.122-0.825). The number of cell lines in the EGFR mutatant-type is five, wild-type is nine. We obtained a mean coverage of 5425 proteins from fourteen lung adenocarcinoma cell lines. There are thirty-nine different expression proteins in total. Significant differences of SF2 was found among fourteen cell lines (P=0.004 < 0.05). showed that there is no correlation between the EGFR mutant-type group and the EGFR wild-type group（P=0.822> 0.05）. The R language analyzed based on proteomic explored that there were thirty-nine DEPs(differently expresse proteins) and four KEGG pathways: ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, and Prion diseases. LAMC1, ITGB4/ITGA6, and CD44 participated in multiple signaling pathways. PPI examined that LAMC1, ITGB4/ITGA6, CD44, FLNB, CD59, PRNP interacted more concentratedly.Conclusion: The inherent radiosensitivity of lung adenocarcinoma cell lines is not determined by the status of EGFR mutant, maybe by other signal pathways such as LAMC1, ITGB4/ITGA6, CD44, and PRNP.
Basal and Luminal Molecular Subtypes in Naturally-Occurring Canine Urothelial Carcinoma are Associated with Tumor Immune Signatures and Dog Breed

BACKGROUND: Improved therapies are needed for patients with invasive urothelial carcinoma (InvUC). Tailoring treatment to molecular subtypes holds promise, but requires further study, including studies in pre-clinical animal models. Naturally-occurring canine InvUC harbors luminal and basal subtypes, mimicking those observed in humans, and could offer a relevant model for the disease in people. OBJECTIVE: To further validate the canine InvUC model, clinical and tumor characteristics associated with luminal and basal subtypes in dogs were determined, with comparison to findings from humans. METHODS: RNA sequencing (RNA-seq) analyses were performed on 56 canine InvUC tissues and bladder mucosa from four normal dogs. Data were aligned to CanFam 3.1, and differentially expressed genes identified. Data were interrogated with panels of genes defining luminal and basal subtypes, immune signatures, and other tumor features. Subject and tumor characteristics, and outcome data were obtained from medical records. RESULTS: Twenty-nine tumors were classified as luminal and 27 tumors as basal subtype. Basal tumors were strongly associated with immune infiltration (OR 52.22, 95%CI 4.68–582.38, P = 0.001) and cancer progression signatures in RNA-seq analyses, more advanced clinical stage, and earlier onset of distant metastases in exploratory analyses (P = 0.0113). Luminal tumors were strongly associated with breeds at high risk for InvUC (OR 0.06, 95%CI 0.01 –0.37, P = 0.002), non-immune infiltrative signatures, and less advanced clinical stage. CONCLUSIONS: Dogs with InvUC could provide a valuable model for testing new treatment strategies in the context of molecular subtype and immune status, and the search for germline variants impacting InvUC onset and subtype.
Pre-emptive gabapentin versus pregabalin for acute postoperative pain management in head and neck surgeries under general anaesthesia

Background: Gabapentin and pregabalin are antiepileptic drugs which are being used in the treatment of neuropathic pain as well as postoperative pain. However there is paucity of studies in comparison with each other in patients undergoing head and neck surgeries under general anaesthesia. Objectives: To evaluate and compare the efficacy of gabapentin and pregabalin with respect to increase in duration of analgesia, reduction in postoperative pain scores, total postoperative analgesic requirements and side effects in patients undergoing head and neck surgeries under general anaesthesia. Settings and design: A prospective, double-blinded, randomized controlled trial. Methods: 100 patients of either gender in ASA I and II were randomly allocated to one of the two groups of fifty each. Patients in group G received single dose of gabapentin 1200mg while in group P received pregabalin 300mg, 1 hour before induction of anaesthesia. Postoperatively, rescue analgesic was administered at visual analogue scale (VAS) >4. The VAS score, sedation score, time to rescue analgesia, total analgesic consumption and side effects were recorded. Results: ln the 24 hours of postoperative period, the mean VAS score of group P was significantly less than group G. Time for rescue analgesic requirement was more with group P than group G. Mean dose of tramadol administered in group P was significantly less when compared to group G. Sedation score was similar in both the groups. No significant difference was observed among the two groups regarding side effects. Conclusion: Pregabalin group had lower VAS score, prolonged timing of first rescue analgesic, and less analgesic consumption than the gabapentin group. Both the groups had similar sedation score with very negligible side effects.
Prototype perception of physically active peers: result from a study among Hungarian university students

Introduction: Previous studies argued the role of social prototypes in making desicisions on healthy and unhealthy behaviors. Positive images associated with a certain behavior might increase the possibility of developing the behavior. While most of the studies focused on health risk behaviors, health promoting behaviors have been less investigated yet. However, young adults are more conscious about the social images associated with health behaviors compared with their younger equivalents; much of the relevant research has been conducted with the latter groups. Purpose: The main aim of this study was to (1) examine how university students perceive their physically active peers, (2) map the adjectives associated with the ‘physically active peers’ prototypes, (3) identify respondents’ categories based on their prototype perception, and (4) detect relationships between social images, sociodemographic variables, sporting habits and social attitudes. Material and Method: Cross-sectional, self-report data were obtained from a convenience sample of 515 respondents aged 18-31 years via a survey with students from a Hungarian university. Result: Cluster analyses detected three independent clusters: “Negative” image” group, “Positive appearance” group, “Positive appearance and personality” group. Age, gender, selfassessed SES, sporting friends, social support, social network, Internet use, competitiveness and the need to belonging seemed to make a difference along these groups of social images. Conclusion: Our findings suggested that social variables may play a moderate role in university students’ sport-related prototype
Validation of a Multiplex Tandem Mass Spectrometry Method for the Detection of Selected Lysosomal Storage Diseases in Dried Blood Spots

Background:Interest in screening methods for lysosomal storage diseases (LSDs) has increased in recent years, since early diagnosis and treatment are essential to prevent or attenuate the onset of symptoms and the complications of these diseases. In the current work, we evaluated the performance of tandem mass spectrometry (MS/MS) for the detection of some LSDs, aiming the future use of this methodology for the screening of these disorders.Methods:Standard curves and quality control dried blood spots were assayed to evaluate the precision, linearity, and accuracy. A total of 150 controls were grouped according to age and subjected to measurement of lysosomal enzymes deficient in Niemann-Pick A/B, Krabbe, Gaucher, Fabry, Pompe, and Mucopolysaccharidosis type I diseases. Samples from 59 affected patients with a diagnosis of LSDs previously confirmed by fluorimetric methods were analyzed.Results:Data from standard calibration demonstrated good linearity and accuracy and the intra- and interassay precisions v.
Comparison between traditional resistance exercise and variable resistance with elastic bands in acute vertical jump performance

Purpose. resistance exercise (rE) with variable resistance (Vr) may be an interesting strategy to increase strength and power in several sports activities. the present study aimed to compare the effect of rE vs. Vr exercise in the back squat on subsequent jump performance at different time points. Methods. Nine male elite handball athletes (age: 21.4 ± 2.1 years; body fat percentage: 10.5 ± 4.2%) undergoing in-season training participated in the study. they randomly completed 3 sets of 5 back squat repetitions at 85% of predicted one-repetition maximum (1rM) with 3-minute rests between sets. they performed rE back squat alone or Vr back squat by combining 55% of 1rM using standard weight plates and 29.7 ± 2.0% of the athlete’s 1rM in fully erect position (starting position) with elastic band resistance. Countermovement jump (CMJ) test was applied at baseline, immediately after the conditioning activity (within 15 seconds), and every 2 minutes after the protocols (2, 4, 6, and 8 minutes). Results. there was a very large (5.8%, p = 0.02, ES = 1.53) increase in CMJ performance 2 minutes after Vr back squat, while no significant changes were observed after the completion of rE back squat. Moreover, there was a large ( p = 0.04, ES = 1.27) increase in peak power after Vr exercise. Conclusions. CMJ performance and peak power output can be potentiated after 2 minutes of recovery following the completion of back squat exercise combining rE plus elastic bands, reinforcing the practical applicability of Vr exercise.
Relentless Border Walls: Challenges of Providing Services and Supports to Migrant Agricultural Workers in British Columbia

Abstract:Temporary migrant workers represent a large portion of the labour force of Canada’s agricultural industry with over 50,000 entering the country each year. Despite well-documented barriers, there have not been standardized and proactive measures to prevent and quickly respond to positive COVID-19 cases among this group who have experienced some of the largest outbreaks outside of long-term care facilities. We conducted research in the Okanagan Valley, British Columbia between 2017–2020, with formal and informal support persons of migrant agricultural workers including members of government agencies, non-profits, and community groups. We found that migrant agricultural workers’ access to support and services is constrained by a series of socio-political forces that we have termed “Relentless Border Walls”. In this paper we define this concept and discuss three key factors that constitute it: onus on workers; paternalism and control; and system-enabled vulnerabilities. Ultimately, these concepts highlight how migrant agricultural workers are made uniquely susceptible to COVID-19 outbreaks, and suggest necessary social and political changes to better address the needs of this population. Résumé:Les travailleurs immigrants temporaires représentent une grande partie de la main-d’œuvre de l’industrie agricole canadienne, avec plus de 50 000 personnes entrant au pays chaque année. Malgré des obstacles bien répertoriés, il n’y a pas eu de mesures normalisées et proactives pour prévenir et réagir rapidement aux cas positifs de COVID-19 au sein de ce groupe qui a connu une des plus importantes éclosions en dehors des centres d’hébergement et de soins de longue durée. Nous avons mené des recherches dans la vallée de l’Okanagan, en Colombie-Britannique, entre 2017 et 2020, avec des personnes de soutien officielles et officieuses de travailleurs agricoles immigrants, y compris des membres d’organismes gouvernementaux, d’organismes sans but lucratif et de groupes communautaires. Nous avons constaté que l’accès des travailleurs agricoles immigrants au soutien et aux services est limité par une série de forces sociopolitiques que nous avons qualifiées de « murs frontaliers draconiens ». Dans cet article, nous définissons ce concept et discutons de trois facteurs clés qui le constituent : le fardeau des travailleurs ; le paternalisme et le contrôle ; et les vulnérabilités du système. En fin de compte, ces concepts mettent en évidence comment les travailleurs agricoles immigrants sont particulièrement vulnérables aux épidémies de COVID-19, et suggèrent les changements sociaux et politiques nécessaires pour mieux répondre aux besoins de cette population.
Fundamentals for Cracking Verification of Reinforced Concrete Structures

A mathematical model, named as general method, has been set up to study the cracking behaviour of a concrete tie reinforced by ordinary bars, subjected to a monotonic load. The analysis is performed considering both the crack formation stage and the stabilized cracking stage. In particular, secondary cracks occur in the stabilized cracking stage, modifying the distribution of bond stress along the transmission length. As in the stabilized cracking stage the solution of the general method requires to carry out an iterative numerical procedure, for design purposes the maximum crack width can be determined through a simplified method that allows the main aspects of the structural behaviour to be taken into account.
FISH FAUNA COMPOSITION, ABUNDANCE AND DISTRIBUTION OF FORCADOS RIVER ESTUARY

This study was carried out to determine the fish fauna composition, abundance and distribution of Forcados river estuary. The monthly fish samples were collected for 24 months between April, 2016 and March, 2018, by using dragnet of stretched mesh sizes 6 mm, 15 mm and 25 mm, gill net and hand net through the services of motorised local artisanal fishers. A total of twelve thousand seven hundred and eighty fishes belonging to 18 families, 27 genera and 34 species were collected. Artisanal fishers should be educated on the importance of sustainable fishing practice to encourage the conservation of fishes in the estuary.
Can Job Satisfaction Mediate the Relationship between Emotional Intelligence and Spiritual Intelligence on Teacher Performance?

This study aims to analyze the role of job satisfaction in mediating the relationship between emotional intelligence and intellectual intelligence on the performance of the high school teachers. This study uses a research design with an associative quantitative approach. Data was collected through documentation and online questionnaires. This study used a sample of 39 respondents with the determination of the sample size using the saturated sample formula. Partial least square is applied to examine the relationship between teacher performance, job satisfaction, emotional intelligence and intellectual intelligence. The results of this study indicate that of the seven hypotheses developed there are two accepted hypotheses, that is, for the effect of emotional intelligence on job satisfaction, it is obtained that the results of a significant effect and the influence of spiritual intelligence on teacher performance are also obtained significant results. Meanwhile, the other 5 hypotheses developed were not significant. Through the findings of this study, it is hoped that it can provide additional information for various parties, especially the school, to pay attention to the factors that affect teacher performance and job satisfaction, so that teachers gain comfort and confidence to continue to improve their performance in implementing learning in schools.
Evaluation of Online Language Exchange Platforms: The Example of 'Free4Talk'

This study aims to evaluate online language exchange platforms and its main focus is on the website named “www.free4talk. com” as an example. The theoretical framework is based on self-directed learning, e-tandem language learning, community of inquiry and expressive language competency. Interviews with the founder, administrator and some of the users of the website are conducted and a 5-point Likert type assessment tool with 26 items and 7 dimensions is created with the help of a literature review at the same time. The findings are obtained through analysing the data collected by applying the instrument to 413 people on the website. The research reveals that this platform presents a free environment with a certain amount of safety and it contributes greatly to foreign language development of learners in terms of providing an opportunity for language practice, an undeniable improvement in expressive language competency and a social atmosphere that offers a source of motivation for language learning for both people who finished their formal foreign language training and for students who are still studying at schools.
Reactive derivatives of gramicidin enable light- and ion-modulated ion channels

Detection of chemical processes on a single molecule scale is the ultimate goal of sensitive analytical assays. We have explored methods to detect chemical analytes in solution using synthetic derivatives of gramicidin A (gA). We exploited the functional properties of an ion channel-forming peptideg—gA—to report changes in the local environment near the opening of these semi-synthetic nanopores upon exposure to specific external stimuli. These peptide-based nanosensors detect reaction-induced changes in the chemical or physical properties of functional groups presented at the opening of the pore. This paper discusses the development of gA-based sensors for detecting external factors such as metal ions in solution or for detecting specific wavelengths of light. We propose that gA-based ion channel sensors offer tremendous potential for ultra sensitive functional detection since a single chemical modification of each individual sensing element can lead to readily detectable changes in channel conductance.
Mathematical modeling of starch gelatinization and some quality properties of parboiled rice based on parboiling indicators using RSM

The effects of parboiling indicators (soaking temperature and steaming time) on the degree of starch gelatinization (DSG) and several rice quality properties [head rice yield (HRY), color value, lightness, and hardness] of parboiled rice were investigated. A mathematical model was used to predict DSG and rice quality from the parboiling conditions, which were varied as follows: 60, 65, and 70 °C for the soaking temperature and 2, 6, and 10 min for the steaming time (at 96 °C). A central composite design was applied and a total of 13 experimental runs were generated for each step. An ANOVA test showed the all models were significant (p < 0.01) and there was no significant lack of fit in any of the response variables. The most appropriate model for prediction the amount of DSG, HRY, color value, lightness, and hardness of samples was linear (R2 = 0.87), quadratic (R2 = 0.92), quadratic (R2 = 0.97), quadratic (R2 = 0.86), and linear (R2 = 0.94) model, respectively. The DSG and hardness increased with increasing soaking temperature and steaming time, while the HRY and lightness showed a maximum at a steaming time of 6 min. The statistical model was used to predict the optimal parboiling conditions for global optimization of the quality properties. Practical applications Parboiled rice enjoys high popularity because of its high nutritional content. The most important effect of parboiling is rice gelatinization. The degree of starch gelatinization (DSG) and quality properties (head rice yield, color value, lightness, and hardness) of parboiled rice are determined by the parboiling indicators (PI): soaking temperature and steaming time. These properties can be predicted based on PI using mathematical models. Increasing the parboiling indicators increases the cost of parboiling process. For the parboiling industry, it will help to determine the soaking temperature and steaming time of rice to yield the desired product quality. For this reason, optimizing the parboiling process is economically important for the rice industry.
Role of pre-operative frailty status in relation to outcome after carotid endarterectomy: a systematic review

Carotid endarterectomy (CEA) is a surgical treatment option to prevent ischemic cerebrovascular accidents. Patients that present with pre-operative frailty might have an elevated risk for unfavorable outcomes after the CEA. A systematic search, using Medline, Embase, Web of Science and Cochrane Database, was performed for relevant literature on frailty in patients undergoing CEA. The study protocol was registered with PROSPERO (CRD42020190345). Eight articles were included. The pooled prevalence for pre-operative frailty was 23.9% (95% CI: 12.98–34.82). A difference in the incidence of complications between frail and non-frail patients (6.4% vs. 5.2%, respectively) and a difference in hospital length of stay [2 (IQR: 2–3) days vs. 1 (IQR: 1–2) day, respectively] were described. The 30-day mortality after CEA was 0.6% for non-frail patients, 2.6% for frail patients, and 4.9% for very frail patients (P<0.001). For 3-year mortality, a >1.5-fold increased risk was found for frail patients (OR 1.7, 95% CI: 1.4–2.0) and a >2.5-fold increased risk for very frail patients (OR 2.6, 95% CI: 2.2–3.1). In conclusion, this review shows the impact of frailty on outcome after CEA. Pre-operative frailty assessment with a validated, multi-domain tool should be implemented in the clinical setting as it will provide information on post-operative surgical outcomes and mortality risk but also frailty trajectory and cognitive decline.