Miracle-dz/UniFAR-FTUs huggingface dataset:
A review of the biology and therapeutic implications of cancer-associated fibroblasts (CAFs) in muscle-invasive bladder cancer

Cancer-associated fibroblasts (CAFs) play a fundamental role in the development of cancers and their response to therapy. In recent years, CAFs have returned to the spotlight as researchers work to unpick the mechanisms by which they impact tumour evolution and therapy responses. However, study of CAFs has largely been restricted to a select number of common cancers, whereas research into CAF biology in bladder cancer has been relatively neglected. In this review, we explore the basics of CAF biology including the numerous potential cellular origins of CAFs, alongside mechanisms of CAF activation and their diverse functionality. We find CAFs play an important role in the progression of bladder cancer with significant implications on tumour cell signaling, epithelial to mesenchymal transition and the capacity to modify components of the immune system. In addition, we highlight some of the landmark papers describing CAF heterogeneity and find trends in the literature to suggest that the iCAF and myCAF subtypes defined in bladder cancer share common characteristics with CAF subtypes described in other settings such as breast and pancreatic cancer. Moreover, based on findings in other common cancers we identify key therapeutic challenges associated with CAFs, such as the lack of specific CAF markers, the paucity of research into bladder-specific CAFs and their relationship with therapies such as radiotherapy. Of relevance, we describe a variety of strategies used to target CAFs in several common cancers, paying particular attention to TGFβ signaling as a prominent regulator of CAF activation. In doing so, we find parallels with bladder cancer that suggest CAF targeting may advance therapeutic options in this setting and improve the current poor survival outcomes in bladder cancer which sadly remain largely unchanged over recent decades.
Pre- and postsynaptic alterations in the visual cortex of the P23H-1 retinal degeneration rat model

P23H rats express a variant of rhodopsin with a mutation that leads to loss of visual function with similar properties as human autosomal dominant retinitis pigmentosa (RP). The advances made in different therapeutic strategies to recover visual system functionality reveal the need to know whether progressive retina degeneration affects the visual cortex structure. Here we are interested in detecting cortical alterations in young rats with moderate retinal degeneration, and in adulthood when degeneration is severer. For this purpose, we studied the synaptic architecture of the primary visual cortex (V1) by analyzing a series of pre- and postsynaptic elements related to excitatory glutamatergic transmission. Visual cortices from control Sprague Dawley (SD) and P23H rats at postnatal days 30 (P30) and P230 were used to evaluate the distribution of vesicular glutamate transporters VGLUT1 and VGLUT2 by immunofluorescence, and to analyze the expression of postsynaptic density protein-95 (PSD-95) by Western blot. The amount and dendritic spine distribution along the apical shafts of the layer V pyramidal neurons, stained by the Golgi-Cox method, were also studied. We observed that at P30, RP does not significantly affect any of the studied markers and structures, which suggests in young P23H rats that visual cortex connectivity seems preserved. However, in adult rats, although VGLUT1 immunoreactivity and PSD-95 expression were similar between both groups, a narrower and stronger VGLUT2-immunoreactive band in layer IV was observed in the P23H rats. Furthermore, RP significantly decreased the density of dendritic spines and altered their distribution along the apical shafts of pyramidal neurons, which remained in a more immature state compared to the P230 SD rats. Our results indicate that the most notable changes in the visual cortex structure take place after a prolonged retinal degeneration period that affected the presynaptic thalamocortical VGLUT2-immunoreactive terminals and postsynaptic dendritic spines from layer V pyramidal cells. Although plasticity is more limited at these ages, future studies will determine how reversible these changes are and to what extent they can affect the visual system’s functionality.
Dynamics of WIMPs in the solar system and implications for detection

Semi-analytic treatments of the evolution of orbits of weakly interacting massive particles (WIMPs) in the solar system suggest that the WIMPs bound to the solar system may enhance the direct detection rate relative to that of the unbound population by up to a factor of order unity, and boost the flux of neutrinos from WIMP annihilation in the Earth by up to two orders of magnitude. To test these important but uncertain results, we perform a suite of numerical orbit integrations (10^5-10^6 particles for up to 4.5 Gyr) to explore the properties of the bound WIMP population as a function of the WIMP mass and the scattering cross section with baryonic matter. For regions of WIMP parameter space presently allowed by experiments, we find that (i) the bound WIMP population enhances the direct detection rate by at most ~1% relative to the rate from unbound halo WIMPs; (ii) it is unlikely that planned km^3-scale neutrino telescopes will detect neutrinos from WIMP annihilation in the Earth; (iii) the event rate from neutrinos produced by WIMP annihilation in the Sun may be much smaller than implied by the usual calculations, which assume that WIMPs scattered onto bound orbits are rapidly thermalized in the Sun.
Single-cell RNA sequencing reveals distinct transcriptional features of the purinergic signaling in mouse trigeminal ganglion

Trigeminal ganglion (TG) is the first station of sensory pathways in the orofacial region. The TG neurons communicate with satellite glial cells (SGCs), macrophages and other cells forming a functional unit that is responsible for processing of orofacial sensory information. Purinergic signaling, one of the most widespread autocrine and paracrine pathways, plays a crucial role in intercellular communication. The multidirectional action of purinergic signaling in different cell types contributes to the neuromodulation and orofacial sensation. To fully understand the purinergic signaling in these processes, it is essential to determine the shared and unique expression patterns of genes associated with purinergic signaling in different cell types. Here, we performed single-cell RNA sequencing of 22,969 cells isolated from normal mouse TGs. We identified 18 distinct cell populations, including 6 neuron subpopulations, 3 glial subpopulations, 7 immune cell subpopulations, fibroblasts, and endothelial cells. We also revealed the transcriptional features of genes associated with purinergic signaling, including purinergic receptors, extracellular adenosine triphosphate (eATP) release channels, eATP metabolism-associated enzymes, and eATP transporters in each cell type. Our results have important implications for understanding and predicting the cell type-specific roles of the purinergic signaling in orofacial signal processing in the trigeminal primary sensory system.
An examination of dispositional social needs, agent knowledge, and two dimensions of product anthropomorphism: A serial mediation model

Thriving attention has been paid to the process and concept of anthropomorphism in marketing literature, as the concept is considered to be a precursor of positive marketing outcomes. However, prior studies have not clarified the position or role of inductive reasoning and anthropomorphism or explained the relationship between anthropomorphism and consumers' individual dispositions. This paper aims to delve into the relationship between consumer psychological and dispositional motivational traits for a given product advertisement that has been personified and imbued with human body features. Building on the literature, a conceptual model has been proposed in which the psychological process-agent knowledge and dispositional motivation to meet social needs have been taken as independent variables positively related to one another and also related individually to the two distinct dimensions of anthropomorphism (i) physical anthropomorphism and (ii) anthropomorphic thinking. Furthermore, it was empirically tested if these two dimensions and these independent variables are linked in a sequential manner. The results show that the need for belonging is positively associated with agent knowledge acquisition, physical anthropomorphism, and anthropomorphic thinking for a given stimulus. Similarly, agent knowledge induced by a humanized stimulus was also positively associated with the two dimensions of anthropomorphism. Furthermore, the two dimensions had a positive relationship with one another. Finally, the need for belonging is also positively associated with agent knowledge and two dimensions of anthropomorphism in a sequential manner. Findings indicate that marketers need to take into account dispositional and psychological factors which might ultimately affect their anthropomorphic inferences in order to induce anthropomorphic thinking because of which positive marketing outcomes take place.
Neural pathways of phonological and semantic processing and its relations to children’s reading skills

Behavioral research shows that children’s phonological ability is strongly associated with better word reading skills, whereas semantic knowledge is strongly related to better reading comprehension. However, most neuroscience research has investigated how brain activation during phonological and semantic processing is related to word reading skill. This study examines if connectivity during phonological processing in the dorsal inferior frontal gyrus (dIFG) to posterior superior temporal gyrus (pSTG) pathway is related to word reading skill, whereas connectivity during semantic processing in the ventral inferior frontal gyrus (vIFG) to posterior middle temporal gyrus (pMTG) pathway is related to reading comprehension skill. We used behavioral and functional magnetic resonance imaging (fMRI) data from a publicly accessible dataset on OpenNeuro.org. The research hypotheses and analytical plan were pre-registered on the Open Science Framework. Forty-six children ages 8–15 years old were included in the final analyses. Participants completed an in-scanner reading task tapping into phonology (i.e., word rhyming) and semantics (i.e., word meaning) as well as standardized measures of word reading and reading comprehension skill. In a series of registered and exploratory analyses, we correlated connectivity coefficients from generalized psychophysiological interactions (gPPI) with behavioral measures and used z-scores to test the equality of two correlation coefficients. Results from the preregistered and exploratory analyses indicated weak evidence that functional connectivity of dIFG to pSTG during phonological processing is positively correlated with better word reading skill, but no evidence that connectivity in the vIFG-pMTG pathway during semantic processing is related to better reading comprehension skill. Moreover, there was no evidence to support the differentiation between the dorsal pathway’s relation to word reading and the ventral pathway’s relation to reading comprehension skills. Our finding suggesting the importance of phonological processing to word reading is in line with prior behavioral and neurodevelopmental models.
Systemic thrombolysis in the management of pump thrombosis in patients with left ventricular assist devices

Left ventricular assist device (LVAD) implantation as destination therapy (DT) is a valuable treatment option in patients with end-stage heart failure ineligible for heart transplant. However, this therapy can be complicated by life-threatening pump thrombosis (PT). This case series reports our single-center experience with a structured systemic thrombolysis protocol in case of PT. Consecutive patients undergoing DT LVAD (HVAD, Medtronic, Framingham, MA) implantation between 2010 and April 2021 at our institution were reviewed and those with PT identified. Clinical, laboratory and LVAD specific data were collected and analyzed retrospectively. All patients with PT were treated with systemic thrombolysis according to a structured bedside protocol. Treatment was defined successful if a patient was alive at 30 days follow-up and free of recurrent PT, stroke or device exchange. Fourteen out of 94 patients experienced a PT after LVAD implantation (11%). Systemic thrombolysis was successful in 10 of 14 patients (71%) at 30 days. Two patients died within 30 days due to a hemothorax and multi-organ failure. In three patients treatment was complicated by a major bleeding; twice a hemothorax (one fatal) and one right calf bleeding. No intracerebral hemorrhage was observed. Three patients experienced a thrombotic complication within 30 days; all recurrent PT. Eleven of the 14 DT patients were discharged home after a limited hospital stay after thrombolysis (average of 11 days). In conclusion, systemic thrombolysis may be a reasonable option for life-threatening PT in this vulnerable DT group in whom device exchange is often impossible due to comorbidity.
Sexual double standard: A gender-based prejudice referring to sexual freedom and sexual shyness

The sexual double standard (SDS) consists of judging men and women differently for the same sexual behavior. This study contributes to research on the factors that determine inconsistent adherence to the SDS. It uses a descriptive methodology to analyze the association between individual and contextual factors both with adherence to the SDS, and with four SDS adherence typologies (man-favorable SDS, woman-favorable SDS, egalitarian and ambivalent). A total of 1,206 heterosexual Spanish adults (603 men and 603 women) participated. The mean age in the male sample was 41.7 (SD = 14.25), in the female sample M = 40.84 (SD = 14.24). The results show that the conceptualization of SDS as a gender-based prejudice is valid to understand the bias of ingroup favoritism that SDS implies: adherence to SDS is more related to the identity of the gender role of men (vs. women). In addition, evidence is provided that the normative context and domain of sexual behavior (i.e., sexual freedom or sexual shyness) determine the form that SDS adopts to express itself. The domain of behaviors related to sexual shyness (vs. domain related to sexual freedom) better discriminates between the different four SDS adherence typologies. The importance of adopting different levels of analysis (i.e., individual, intergroup, societal) to explain and predict both SDS adherence and the prevalence of SDS adherence typologies is discussed.
Fortification of cocoa semi-skimmed milk formulations with native lactic acid bacteria: Cell viability, physicochemical and functional properties for developing novel foods

This study aimed to evaluate several cocoa semi-skimmed milk formulations (CSMFs) as potential carriers of native lactic acid bacteria (LAB) strains to obtain novel probiotic beverages (PBs) with improved technological and functional characteristics, and satisfactorily organoleptic acceptance. The viability of two native LAB (Lactiplantibacillus plantarum UTNGt2 and Lactiplantibacillus pentosus UTNGt5) was assessed in comparison with two references (Lactococcus lactis subsp. lactis ATCC11474 and Limosilactobacillus reuteri DSM17938) strains in supplemented CSMFs throughout storage with refrigeration. The optimum conditions to produce novel beverages supplemented with native LAB were pH 6.6, 42°C, and 1 h of fermentation. Moreover, the effect of LAB strains fortification on pH, titratable acidity, total solids (°Brix), total polyphenolic compounds (TPC), antioxidant capacity (AOX), and ascorbic acid content (AAC), total proteins and fat, at initial and final storage was evaluated. The addition of two native LAB strains did alter the physicochemical quality of CSMFs to a lesser extent, where the bioactive molecules improved significantly (p < 0.05) with the increase of cocoa concentration and depending on the supplied strain. Although a statistically significant (p < 0.05) decrease in cell counts was recorded during storage, the LAB cells were found to be viable up to 21 days of storage at 4°C (>6 logCFU/ml), which is sufficient in number to prove their stability in vitro. Overall organoleptic results suggested that LAB supplementation had a significant impact on sensory attributes with satisfactory acceptability (>78%) of PBs containing the native strains and 1–2% cocoa, while CSMFs counterparts were less appreciated (40%) as perceived off-flavor. It appears that supplying bacteria to CSMF preserves flavor in the final product. Furthermore, the final beverages were free of harmful bacteria; thus, they comply with consumer safety regulations. This study concludes that CSMF can be used as a carrier of native LAB strains, maintaining cell viability, unaltered physicochemical properties, and improved functional and sensory characteristics, for which final beverages can be regarded as functional food. From the application standpoint, these formulations are an alternative to delivering native LAB strains and could help the cocoa and dairy industry to develop more attractive products for the growing regional market.
The “beauty premium” effect of voice attractiveness of long speech sounds in outcome-evaluation event-related potentials in a trust game

Previous research suggested that people with attractive voices had an advantage in economic games, even if the voices were only presented for 400 ms. The present study investigated the influence of voice attractiveness on the cooperative trust behavior with longer exposure times to the voices. Event-related potentials (ERPs) were recorded during the feedback outcome evaluation. Participants heard a voice of the partner for 2,040 ms and decided whether to invest to the partner for a possibility to gain more money. The results showed that participants made more invest choices to the attractive partners, replicating the “beauty premium” effect of the attractive voices. Moreover, participants were more likely to invest to male partners. The ERP analysis for the outcome showed that the difference waves of feedback-related negativity (FRN) amplitude were smaller in the attractive voice condition than in the unattractive voice condition, suggesting that the rewarding effect of attractive voices weakened the frustrating feelings of the loss. In sum, the present study confirms that attractive voices with longer presentation durations facilitate cooperative behavior and modulate the processing of feedback evaluations.
The add-on effect of Shufeng Jiedu capsule for treating COVID-19: A systematic review and meta-analysis

Introduction Shufeng Jiedu capsule (SFJD) is a commonly used Chinese patent medicine in China. Some studies have reported that SFJD has therapeutic effects in patients diagnosed with COVID-19. This systematic review aimed to critically evaluate the efficacy and safety of SFJD combined with western medicine (WM) for treating COVID-19. Methods A literature search by using WHO COVID-19 database, PubMed, Embase, Cochrane Library, the Web of Science, CKNI, Wanfang, VIP, SinoMed, and clinical trial registries was conducted, up to 1 August 2022. Randomized controlled trials (RCTs), non-RCTs, cohort studies and case series of SFJD combined with WM for COVID-19 were included. Literature screening, data extraction, and quality assessment were performed independently by two reviewers in line with the same criteria. We used the Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) to assess the certainty of evidence. Meta-analyses were performed with Revman 5.3 if possible. The descriptive analysis was conducted when the studies could not be meta-analyzed. Results Totally 10 studies with 1,083 patients were included. Their methodological quality were moderate. The results demonstrated that compared to WM group, SFJD + WM group remarkably increased the nucleic acid negative conversion rate (RR = 1.40, 95%CI: 1.07–1.84), total effective rate (RR = 1.18, 95%CI: 1.07–1.31), cure rate (RR = 4.06, 95%CI: 2.19–7.53), and the chest CT improvement rate (RR = 1.19, 95%CI: 1.08–1.31), shorten nucleic acid negative conversion time (MD = −0.70, 95%CI: −1.14 to −0.26), reduced the clinical symptom disappearance time (fever, diarrhea, cough, fatigue, pharyngalgia, nasal congestion, and rhinorrhea), as well as improved the levels of laboratory outcomes (CRP, IL-6, Lym, and Neu). Additionally, the incidence of adverse reactions did not exhibit any statistically significant difference between SFJD + WM group and WM group. Conclusion SFJD combined with WM seems more effective than WM alone for the treatment of COVID-19. However, more well-designed RCTs still are warranted. Systematic review registration [https://www.crd. york.ac. uk/PROSPERO/], identifier [CRD42022306307].
Contribution of cognitive and bodily navigation cues to egocentric and allocentric spatial memory in hallucinations due to Parkinson's disease: A case report

Parkinson's disease (PD) manifestations can include visual hallucinations and illusions. Recent findings suggest that the coherent integration of bodily information within an egocentric representation could play a crucial role in these phenomena. Egocentric processing is a key aspect of spatial navigation and is supported by the striatum. Due to the deterioration of the striatal and motor systems, PD mainly impairs the egocentric rather than the allocentric spatial frame of reference. However, it is still unclear the interplay between spatial cognition and PD hallucinations and how different navigation mechanisms can influence such spatial frames of reference. We report the case of A.A. , a patient that suffers from PD with frequent episodes of visual hallucinations and illusions. We used a virtual reality (VR) navigation task to assess egocentric and allocentric spatial memory under five navigation conditions (passive, immersive, map, path decision, and attentive cues) in A.A. and a PD control group without psychosis. In general, A.A. exhibited a statistically significant classical dissociation between the egocentric and allocentric performance with a greater deficit for the former. In particular, the dissociation was statistically significant in the “passive” and “attentive cues” conditions. Interestingly in the “immersive” condition, the dissociation was not significant and, in contrast to the other conditions, trends showed better performance for egocentric than allocentric memory. Within the theories of embodiment, we suggest that body-based information, as assessed with VR navigation tasks, could play an important role in PD hallucinations. In addition, the possible neural underpinnings and the usefulness of VR are discussed.