Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical and microbiological assessments of COVID-19 in healthcare workers: a prospective longitudinal study

Background A comprehensive assessment of COVID-19 in healthcare workers (HCWs) including the investigation of viral shedding duration is critical. Methods A longitudinal study including 319 HCWs was conducted. After SARS-CoV-2 screening with RT-PCR assay, other respiratory pathogens were tested with a multiplex molecular panel. For SARS-CoV-2 positive HCWs, the normalized viral load was determined weekly; viral culture and virus neutralization assays were also performed. For 190 HCWs tested negative, SARS-CoV-2 serological testing was performed one month after the inclusion. Findings Of the 319 HCWs included, 67 (21.0%) were tested positive for SARS-CoV-2; two of them developed severe COVID-19. The proportion of smell and taste dysfunction was significantly higher in SARS-CoV-2 positive HCWs than in negative ones (38.8% vs 9.5% and 37.3% vs 10.7%, respectively, p<0.001). Of the 67 positive patients, 9.1% were tested positive for at least another respiratory pathogen (vs 19.5%, p=0.07). The proportion of HCWs with a viral load > 5.0 log10 cp/ml (Ct value <25) was less than 15% at 8 days after symptom onset; 12% of them were still positive after 40 days (Ct >37). More than 90% of culturable virus had a viral load > 4.5 log10 cp/ml (Ct < 26) and were collected within 10 days after symptom onset. From HCWs tested negative, 6/190 (3.2%) exhibited seroconversion for IgG antibodies. Interpretation Our data suggest that the determination of normalized viral load (or its estimation through Ct values) can be useful for discontinuing isolation of HCWs and facilitating their safe return to work. HCWs presenting mild COVID-19 are unlikely infectious 10 days after symptom onset.
Analysis of Push and Pull Motivations and the Intentions to Return and Recommend a Coastal or Marine Destination

ABSTRACT Carvache-Franco, M. ; Carvache-Franco, W. ; Carvache-Franco, O. , and Hernández-Lara, A.B. , 2020. Analysis of push and pull motivations and the intentions to return and recommend a coastal or marine destination. Journal of Coastal Research, 36(6), 1313–1322. Coconut Creek (Florida), ISSN 0749-0208. Visiting coastal and marine destinations can be appealing for tourists depending on the on-site activities offered. This study analyzed push and pull motivations to establish their effect on visitors' intention to return and likelihood to recommend a tourist destination. The research was conducted in the city of Salinas in Ecuador, a renowned tourist destination with coastal and marine, natural, and cultural features. For the statistical procedure, factor analysis was used as a data reduction technique, and multiple regression analysis was conducted to select the motivational variables that predict the intentions to return and recommend the destination. The results show two push motivational factors, “escape and novelty,” and “knowledge and experience,” and two pull motivational factors, “active marine,” and “static marine. ” The first pull motivational factor is related to marine sports, swimming in the ocean, and adventurous activities, and the second one is associated with clear water and fresh air enjoyment, walking along the beach, and beaches of scenic beauty. The push factor “escape and novelty” and the pull factor “static marine” were the most important predictors of the intentions to return and recommend a coastal and marine destination. These findings will help coastal and marine planners to implement sustainable development strategies according to the characteristics of the demand.
Scientometrics of the genus Macrobrachium by using the regional PERIODICA database

In order to provide information on the development status of the Macrobrachium genus study in Latin America, the results of the scientometric analysis of 161 documents published between 1976 and 2016 in journals of the region, managed from the PERIODICA database, are presented. The current status of the genus Macrobrachium is described in the above geographical area. Results are presented on the years of generation of articles, species studied, main themes treated, journals, most representative authors, countries and language. The most productive countries are Brazil and Mexico. The contents were written in Portuguese as the preponderant language, followed by Spanish. The main topics covered are culture, ecology, food-nutrition, physiology and reproduction, and the least discussed were biology, pathology, food technology, genetics, molecular biology and behavior. The most studied species are M. rosenbergii, M. acanthurus, M. carcinus and M. amazonicum. The most representative authors are V.L. Lobao, W.C. Valenti, J.V. Lombardi and C. Graziani.
Multiresolution Satellite-Derived Bathymetry in Shallow Coral Reefs: Improving Linear Algorithms with Geographical Analysis

ABSTRACT da Silveira, C.B. L. ; Strenzel, G.M. R. ; Maida, M. ; Araújo, T.C. M. , and Ferreira, B.P., 2020. Multiresolution satellite-derived bathymetry in shallow coral reefs: Improving linear algorithms with geographical analysis. Journal of Coastal Research, 36(6), 1247–1265. Coconut Creek (Florida), ISSN 0749-0208. Bathymetric maps are one of the first steps for most hydrological and ecological studies of the seascape, as depth is a determinant factor in the distribution of organisms, patterns of wave exposure, and coastal circulation. The coral reefs of Tamandaré-Brazil, located at Costa dos Corais marine protected area (MPA), encompass a mosaic of interconnected habitats of complex geomorphology, including coral reefs, algal, and seagrass beds. These coastal habitats are subjected to chronic impacts such as sedimentation, reef erosion, and increasing human use, leading to habitat loss. Despite their social and ecological importance and the conservation measures in place, bathymetric and habitat maps of this coast are lacking. Indeed, in situ surveys are not always feasible in shallow coral reef areas. The present study offers a detailed bathymetric mapping of the area using multiresolution satellite imagery. One Landsat-8 (December 2016) and one WorldView-03 (February 2017) imagery were used to derive medium (30-m) and high-resolution (2-m) bathymetry of the study area. Single-beam echo sounder surveys were performed to obtain field data to calibrate the depth-retrieving algorithms: linear model, ratio band model, principal component analyses of the transformed bands, and geographically weighted regressions (GWR). For both resolution datasets, results showed that the algorithms' RMS were significantly improved by the GWR technique (RMS > 0.9) because of adaptability to the bottom heterogeneity found in complex areas such as coral reefs. Specific geomorphological reef zones were recognizable in the resulting bathymetric maps, such as intrareef lagoon, reef crest, fore-reef, and reef flat. This research concluded that affordable methods such as single-beam data coupled with satellite imagery through GWR can provide the required inputs for mapping shallow areas with complex relief. Such results may be further used to habitat mapping, necessary to inform the multiple-use zonation foreseen in MPA management plans.
Motivation States for Physical Activity and Sedentary Behavior: Desire, Urge, Wanting, and Craving

To better explain daily fluctuations in physical activity and sedentary behavior, investigations of motivation are turning from social cognitive frameworks to those centered on affect, emotion and automaticity, such as the Affect and Health Behavior Framework (AHBF), Integrated Framework and Affective-Reflective Theory (ART). This shift has necessitated: (a) re-examination of older theories and their constructs, such as drives, needs and tensions and (b) an inspection of competing theories from other fields that also attempt to explain dynamic changes in health behaviors. The Dynamical Model of Desire, Elaborated Intrusion Theory and others commonly share with AHBF the idea that human behavior is driven strongly by desires and/or the similar concepts of wants, urges, and cravings. These affectively-charged motivation states (ACMS) change quickly and may better explain physical activity behavior from one moment to the next. Desires for movement predominantly derive from negative but also positive reinforcement. Data from clinical populations with movement dysfunction or psychiatric disorders provides further evidence of these drivers of movement. Those with Restless Legs Syndrome, akathisia, tic disorders and exercise dependence all report strong urges to move and relief when it is accomplished. Motor control research has identified centers of the brain responsible for wants and urges for muscular movement. Models elaborated herein differentiate between wants, desires, urges and cravings. The WANT model (Wants and Aversions for Neuromuscular Tasks) conceptualizes desires for movement and rest as varying by magnitude, approach or avoidance-orientation (wants versus aversions) and as occupying independent dimensions instead of opposite ends of the same axis. For instance, one hypothetically might be in a state of both high desire for movement and rest simultaneously. Variations in motivation states to move and rest may also be associated with various stress states, like freezing or fight and flight. The first validated instrument to measure feelings of desire/want for movement and rest, the CRAVE Scale (Cravings for Rest and Volitional Energy Expenditure) is already shedding light on the nature of these states. With these advances in theory, conceptual modeling and instrumentation, future investigations may explore the effects of desires and urges for movement and sedentary behavior in earnest.
Description of a new species of Gaeolaelaps Evans & Till (Mesostigmata: Laelapidae) from northern Iran, supplementary information on G. deinos (Zeman) and redescription of G. schusteri (Hirschmann)

Abstract A new species of the laelapid mite genus Gaeolaelaps Evans & Till, G. tuberculatus sp. nov., is described from female specimens collected in soil and rotten leaves under citrus trees in Mazandaran province, northern Iran. Supplementary information on G. deinos (Zeman) is presented. Gaeolaelaps schusteri (Hirschmann) is redescribed based on the type specimens as well as additional specimens collected from the littoral zone of mangrove forests in Qeshm Island, in the eastern part of the Persian Gulf, southern Iran.
Adjuvants for Coronavirus Vaccines

Vaccine development utilizing various platforms is one of the strategies that has been proposed to address the coronavirus disease 2019 (COVID-19) pandemic. Adjuvants are critical components of both subunit and certain inactivated vaccines because they induce specific immune responses that are more robust and long-lasting. A review of the history of coronavirus vaccine development demonstrates that only a few adjuvants, including aluminum salts, emulsions, and TLR agonists, have been formulated for the severe acute respiratory syndrome-associated coronavirus (SARS-CoV), Middle East respiratory syndrome-related coronavirus (MERS-CoV), and currently the SARS-CoV-2 vaccines in experimental and pre-clinical studies. However, there is still a lack of evidence regarding the effects of the adjuvants tested in coronavirus vaccines. This paper presents an overview of adjuvants that have been formulated in reported coronavirus vaccine studies, which should assist with the design and selection of adjuvants with optimal efficacy and safety profiles for COVID-19 vaccines.
Risperidone-Induced Obesity in Children and Adolescents With Autism Spectrum Disorder: Genetic and Clinical Risk Factors

Aims: Obesity is a significant problem for patients taking atypical antipsychotics. There were two aims of our study. The first aim was to compare the prevalence of overweight and obesity between children and adolescents with autism spectrum disorder (ASD) treated with risperidone with the general pediatric population. The second aim was to investigate the association of the HTR2C -759C>T, ABCB1 1236C>T, ABCB1 2677G>T/A, and ABCB1 3435C>T polymorphisms with risperidone-induced overweight and obesity in children and adolescents with ASD. Methods: Body weight and height were measured in 134 subjects. Overweight and obesity in children and adolescents were classified using the International Obesity Task Force (IOTF) criteria. Genotyping was performed by TaqMan real-time polymerase chain reaction (PCR). Results: Our study found that the prevalence of overweight and obesity was significantly higher in children and adolescents with ASD treated with risperidone compared with healthy individuals (p = 0.01 and p = 0.002). The genetic polymorphisms of HTR2C –759C>T, ABCB1 1236C>T, ABCB1 2677G>T/A, and ABCB1 3435C>T were not associated with overweight/obesity in children and adolescents with ASD treated with risperidone after adjustment for multiple comparisons by the method of Bonferroni. Additionally, haplotype analysis revealed that there was no significant association between ABCB1 3435T-2677T/A-1236T haplotype and overweight/obesity. In multivariate logistic regression, after adjustment by the Bonferroni correction, there was only the duration of risperidone treatment that was significantly associated with overweight/obesity in children and adolescents with ASD. Conclusions: The findings suggest that children and adolescents with ASD treated with risperidone are at a higher risk of obesity, especially patients with extended treatment with risperidone. For the pharmacogenetic factors, –759C>T polymorphism of HTR2C gene and 1236C>T, 2677G>T/A, and 3435C>T polymorphisms of ABCB1 gene were not likely to be associated with the susceptibility to overweight/obesity in children and adolescents treated with risperidone. Due to the small sample size, further studies with a larger independent group are needed to confirm these findings.
Corneal Densitometry After Small Incision Lenticule Extraction (SMILE) and Femtosecond Laser-Assisted LASIK (FS-LASIK): 5-Year Prospective Comparative Study

Purpose: To investigate long-term changes in corneal densitometry (CD) following small incision lenticule extraction (SMILE) vs. femtosecond laser-assisted LASIK (FS-LASIK) in patients with myopia or myopic astigmatism. Methods: Prospective analysis was performed in 66 eyes of 38 patients (13 males) who underwent SMILE and 54 eyes of 29 patients (5 males) who underwent FS-LASIK. In all patients, an ocular examination was performed preoperatively, and at 6–12 months and 5 years postoperatively. CD was obtained with the Pentacam Scheimpflug imaging system at the 0–2-mm, 2–6-mm, and 6–10-mm zones of the cornea at depth of anterior 120 μm, midcornea, and posterior 60 μm. Correlation analysis was performed between postoperative change in CD and other variables such as age, type of surgery, central corneal thickness, spherical equivalent, lenticule thickness/ablation depth, and changes in wavefront aberrations. Results: At postoperative 6–12 months, a significant reduction at several corneal zones in the FS-LASIK cohort (P < 0.05) was observed. In the SMILE cohort, no significant change in CD relative to baseline was observed. However, at 5 years postoperatively, in both groups, a significant decrease in CD was observed in three zones of three layers (all P < 0.001). The change in CD was similar between groups at postoperative 6–12 months, but at 5 years the magnitude of change was significantly smaller in SMILE than FS-LASIK in the anterior and central layers (all P < 0.05). Conclusion: CD with the Scheimpflug imaging system showed a significant decrease at 5 years after SMILE or FS-LASIK, and the change was significantly less pronounced after SMILE.
The Costs of Store Sales for Retail Workers

In the context of economic stagnation and recession, retailers face fierce competition and experience enormous pressure to increase their sales. In this study, we focus on the potential costs of higher store sales for retail workers by examining its effect on work engagement. Drawing on work intensification literature and the job demand-resources model, we study how job variety and workload, two job characteristics, mediate the relationship between store sales and engagement. Store revenue data and survey data of 525 sales employees, embedded in 110 stores of a large Dutch retail organization were used, to perform mixed models analyses. The analyses demonstrate that store sales is negatively related to job variety and positively related to workload. In turn, job variety positively affects work engagement, while workload negatively affects work engagement. Based on multi-source, multilevel data it is thus shown that there are negative effects of store sales in retail. More insight is created into the job characteristics that explain the negative link between store sales and engagement. As it is empirically demonstrated that there are indeed costs associated with improved performance in retail, it is crucial that organizations ensure investments in maintaining resourceful work environments.
The G-protein Coupled Receptor GPR8 Regulates Secondary Metabolism in Trichoderma reesei

Changing environmental conditions are of utmost importance for regulation of secondary metabolism in fungi. Different environmental cues including the carbon source, light and the presence of a mating partner can lead to altered production of compounds. Thereby, the heterotrimeric G-protein pathway is of major importance for sensing and adjustment of gene regulation. Regulation of secondary metabolism is crucial in the biotechnological workhorse Trichoderma reesei for knowledge-based adjustment in industrial fermentations, but also with respect to the potential use as a host for heterologous compound production. We investigated the function of the class VII G-protein coupled receptor (GPCR) gene gpr8 that is localized in the vicinity of the SOR cluster, which is responsible for biosynthesis of sorbicillinoids. GPR8 positively impacts regulation of the genes in this cluster in darkness. Accordingly, abundance of trichodimerol and dihydrotrichotetronine as well as other secondary metabolites is decreased in the deletion mutant. Transcriptome analysis moreover showed the major role of GPR8 being exerted in darkness with a considerable influence on regulation of secondary metabolism. Genes regulated in Δgpr8 overlap with those regulated directly or indirectly by the transcription factor YPR2, especially concerning genes related to secondary metabolism. The predicted FAD/FMN containing dehydrogenase gene sor7, one of the positive targets of the cascade triggered by GPR8, has a positive effect on secondary metabolite production, but also cellulase gene expression. Hence SOR7 has some overlapping, but also additional functions compared to GPR8. The G-protein coupled receptor GPR8 exerts a light dependent impact on secondary metabolism, which is in part mediated by the transcription factor YPR2 and the function of SOR7. Hence, T. reesei may apply GPR8 to adjust production of secondary metabolites and hence chemical communication to signals from the environment.
αCGRP-Induced Changes in Cerebral and Systemic Circulation; A TCD Study

It is known that perivascular application of CGRP induces cerebral vasodilatation. However, it is unclear whether intravenous alfa CGRP (αCGRP) induces changes in cerebral and systemic hemodynamics. Therefore, we studied the influence of an αCGRP intravenous infusion at a rate of 1.5 mcg/min in 20 min on mean arterial velocity in the middle cerebral artery (vm MCA) and in the posterior cerebral artery (vm PCA) in twenty healthy subjects using transcranial Doppler (TCD). We found out that αCGRP decreased vm MCA (p < 0.001), vm PCA (p < 0.001), mean arterial pressure (MAP) (p < 0.001) and end-tidal CO2 (Et-CO2) (p = 0.030). The heart rate (HR) increased during αCGRP infusion (p < 0.001). In addition, we found a positive relationship between Et-CO2 and vm MCA (p = 0.001) as well as vm PCA (p = 0.043). In our view, αCGRP induces changes in cerebral and systemic circulation in healthy volunteers. It might cause vasodilatation of MCA and PCA and a compensatory decrease of Et-CO2 to αCGRP related hemodynamic changes.
Immediate Psychological Effects of the COVID-19 Quarantine in Youth From Italy and Spain

The COVID-19 quarantine has affected more than 860 million children and adolescents worldwide, but to date, no study has been developed within Western countries to examine the psychological impact on their lives. The present study aims to examine for the first time the emotional impact of the quarantine on children and adolescents from Italy and Spain, two of the countries most affected by COVID-19. Participants were 1,143 parents of Italian and Spanish children aged 3 to 18 years who completed a survey providing information about how the quarantine affects their children and themselves, compared to before the home confinement. Results show that 85.7% of the parents perceived changes in their children’s emotional state and behaviors during the quarantine. The most frequent symptoms were difficulty concentrating (76.6%), boredom (52%), irritability (39%), restlessness (38.8%), nervousness (38%), feelings of loneliness (31.3%), uneasiness (30.4%), and worries (30.1%). Spanish parents reported more symptoms than Italians. As expected, children of both countries used monitors more frequently, spent less time doing physical activity, and slept more hours during the quarantine. Furthermore, when family coexistence during quarantine became more difficult, the situation was more serious, and the level of stress was higher, parents tended to report more emotional problems in their children. The quarantine impacts considerably on Italian and Spanish youth, reinforcing the need to detect children with problems as early as possible to improve their psychological well-being.