Miracle-dz/UniFAR-FTUs huggingface dataset:
Phenotypic effects of overexpression of PKCβ1 in rat liver epithelial cells

We have used a previously described retroviral expression vector pMV7‐PKCβ1 to develop derivatives of two rat liver epithelial cell lines, K16 and K22, that stably express about tenfold‐higher PKC activity than control cells. Despite these high levels of PKC, these cells did not exhibit gross morphologic changes, anchorage‐independent growth, or tumorigenicity. K16PKC‐4 and K22PKC‐2, two lines with the highest PKC enzyme activity, were studied further in terms of several responses to the phorbol ester tumor promoter TPA. When treated with 100 ng/ml of TPA, the control K16MV7 and K22MV7 cells displayed a slight change in morphology, whereas the K16PKC‐4 and K22PKC‐2 cells displayed a marked change in morphology. Northern blot analyses demonstrated that TPA induced increased levels of fos, myc, phorbin, and ODC RNAs in control K16MV7 and K22MV7 cells, with maximum induction occurring at about 0.5, 1, 8, and 8 h, respectively. In K16PKC‐4 and K22PKC‐2 cells, TPA induction of phorbin and ODC RNAs was markedly enhanced, but this was not the case for myc and fos RNAs. In addition, the levels of myc RNA were constitutively higher in both K16PKC‐4 and K22PKC‐2 cells than in the control cells. Taken together, these results provide direct evidence that PKC plays a critical role in modulating the expression of myc, phorbin, and ODC RNAs. On the other hand, overexpression of PKCβ1 is not itself sufficient to cause cell transformation.
SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat

The content and composition of seed storage proteins is largely responsible for wheat end-use quality. They mainly consist of polymeric glutenins and monomeric gliadins. According to their electrophoretic mobility, gliadins and glutenins are subdivided into several fractions. Glutenins are classified as high molecular weight or low molecular weight glutenin subunits (HMW-GSs and LMW-GSs, respectively). LMW-GSs are encoded by multigene families located at the orthologous Glu-3 loci. We designed a set of 16 single-nucleotide polymorphism (SNP) markers that are able to detect SDS-PAGE alleles at the Glu-A3 and Glu-B3 loci. The SNP markers captured the diversity of alleles in 88 international reference lines and 27 Mexican cultivars, when compared to SDS-PAGE and STS markers, however, showed a slightly larger percent of multiple alleles, mainly for Glu-B3. SNP markers were then used to determine the Glu-1 and Glu-3 allele composition in 54 CIMMYT historical lines and demonstrated to be useful tool for breeding programs to improve wheat end product properties.
The Aging of γδ T Cells

In the coming decades, many developed countries in the world are expecting the “greying” of their populations. This phenomenon poses unprecedented challenges to healthcare systems. Aging is one of the most important risk factors for infections and a myriad of diseases such as cancer, cardiovascular and neurodegenerative diseases. A common denominator that is implicated in these diseases is the immune system. The immune system consists of the innate and adaptive arms that complement each other to provide the host with a holistic defense system. While the diverse interactions between multiple arms of the immune system are necessary for its function, this complexity is amplified in the aging immune system as each immune cell type is affected differently—resulting in a conundrum that is especially difficult to target. Furthermore, certain cell types, such as γδ T cells, do not fit categorically into the arms of innate or adaptive immunity. In this review, we will first introduce the human γδ T cell family and its ligands before discussing parallels in mice. By covering the ontogeny and homeostasis of γδ T cells during their lifespan, we will better capture their evolution and responses to age-related stressors. Finally, we will identify knowledge gaps within these topics that can advance our understanding of the relationship between γδ T cells and aging, as well as age-related diseases such as cancer.
Long Non-Coding RNA PANTR1 is Associated with Poor Prognosis and Influences Angiogenesis and Apoptosis in Clear-Cell Renal Cell Cancer

POU3F3 adjacent non-coding transcript 1 (PANTR1) is an oncogenic long non-coding RNA with significant influence on numerous cellular features in different types of cancer. No characterization of its role in renal cell carcinoma (RCC) is yet available. In this study, PANTR1 expression was confined to human brain and kidney tissue and was found significantly up-regulated in clear-cell renal cell carcinoma tissue (ccRCC) compared to non-cancerous kidney tissue in two independent cohorts (p < 0.001 for both cohorts). In uni- and multivariate Cox regression analysis, ccRCC patients with higher levels of PANTR1 showed significantly poorer disease-free survival in our own respective cohort (n = 175, hazard ratio: 4.3, 95% confidence interval: 1.45–12.75, p = 0.008) in accordance with significantly poorer overall survival in a large The Cancer Genome Atlas database (TCGA) cohort (n = 530, hazard ratio: 2.19, 95% confidence interval: 1.59–3.03, p ≤ 0.001). To study the underlying cellular mechanisms mediated by varying levels of PANTR1 in kidney cancer cells, we applied siRNA-mediated knock-down experiments in three independent ccRCC cell lines (RCC-FG, RCC-MF, 769-P). A decrease in PANTR1 levels led to significantly reduced cellular growth through activation of apoptosis in all tested cell lines. Moreover, as angiogenesis is a critical driver in ccRCC pathogenesis, we identified that PANTR1 expression is critical for in vitro tube formation and endothelial cell migration (p < 0.05). On the molecular level, knock-down of PANTR1 led to a decrease in Vascular Endothelial growth factor A (VEGF-A) and cell adhesion molecule laminin subunit gamma-2 (LAMC2) expression, corroborated by a positive correlation in RCC tissue (for VEGF-A R = 0.19, p < 0.0001, for LAMC2 R = 0.13, p = 0.0028). In conclusion, this study provides first evidence that PANTR1 has a relevant role in human RCC by influencing apoptosis and angiogenesis.
Factors associated with high-risk behaviors of people newly diagnosed with HIV/AIDS: results from a cross-sectional study in Vietnam

ABSTRACT Towards the elimination of this global epidemic, understanding the high-risk behaviors of people newly diagnosed with HIV/AIDS (PNDWH) is essential. This study aimed to describe the general characteristics and high-risk behaviors of PNDWH and identify associated factors for adopting high-risk behaviors. A cross-sectional survey was conducted in Vietnam to explore the high-risk behaviors of 506 PNDWH. Associated factors were identified using multivariable logistic regressions. 83.2% of participants had sex without using a condom, and 27.9% had more than two sex partners. Among injected drug users, 44% had shared needles with an average number of 2.1 shared partners. Male, Kinh ethnicity (Vietnamese), high income, and high educational level were risk factors for high-risk behaviors. Our findings revealed the first time a comprehensive picture of PNDWH and emphasized the high prevalence of STIs, including untreated STIs and the long delay since the early HIV diagnosis. Also, our model found much higher risk behaviors among participants who were non-adherent to ART and those currently enrolled in ART. By better managing newly-diagnosed cases, better integrating STI management services and prevention consultants, as well as improving ART adherence programs, Vietnam can make better progress towards the complete control of HIV for its most vulnerable populations.
Lake Trout Movements in Northwestern Lake Michigan

Abstract We quantified the distance that lake trout Salvelinus namaycush moved in northwestern Lake Michigan and examined (1) the directional preference and (2) the effect of population density on movement. Lake trout were captured in spring and fall 1983–1996, tagged with Floy anchor tags, and recaptured during subsequent agency sampling and by commercial fishers and anglers during 1983–1997. Angler recaptures were used to quantify movements; these recaptures were standardized to 10,000 salmonid angler-hours (giving recaptures per effort (RPE)) to account for the spatial and temporal variation in recapture effort. Movement was inferred from the spatial and temporal differences in the distribution of RPE. The dispersal radius, an index of the area occupied by tagged lake trout, was defined as the area containing 90% of the total RPE. It was estimated by fitting the cumulative proportion of RPE versus distance from the tagging location to an exponential sigmoid model and using inverse prediction. We used l.
Limonoids from Clausena anisata

Five tetranortriterpenoids have been isolated from the stem bark and roots of Clausena anisata. The known compounds were identified spectroscopically as limonin, zapoterin and clausenolide. The novel compounds include clausenolide-1-ethyl ether and clausemarin. The isolation of these limonoids from C. anisata is of some chemotaxonomic interest
Effect of Humidity on Light-Activated NO and NO2 Gas Sensing by Hybrid Materials

Air humidity is one of the main factors affecting the characteristics of semiconductor gas sensors, especially at low measurement temperatures. In this work we analyzed the influence of relative humidity on sensor properties of the hybrid materials based on the nanocrystalline SnO2 and In2O3 and Ru (II) heterocyclic complex and verified the possibility of using such materials for NO (0.25–4.0 ppm) and NO2 (0.05–1.0 ppm) detection in high humidity conditions (relative humidity (RH) = 20%, 40%, 65%, 90%) at room temperature during periodic blue (λmax = 470 nm) illumination. To reveal the reasons for the different influence of humidity on the sensors’ sensitivity when detecting NO and NO2, electron paramagnetic resonance (EPR) spectroscopy and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) investigations were undertaken. It was established that the substitution of adsorbed oxygen by water molecules causes the decrease in sensor response to NO in humid air. The influence of humidity on the interaction of sensitive materials with NO2 is determined by the following factors: the increase in charge carrier’s concentration, the decrease in the number of active sites capable of interacting with gases, and possible substitution of chemisorbed oxygen with NO2− groups.
Genetic population structure of the net‐winged midge, Elporia barnardi (Diptera: Blephariceridae) in streams of the south‐western Cape, South Africa: implications for dispersal

SUMMARY 1. The net-winged midges (Diptera: Blephariceridae), with highly specific habitat requirements and specialised morphological adaptations, exhibit high habitat fidelity and a limited potential for dispersal. Given the longitudinal and hierarchical nature of lotic systems, along with the geological structure of catchment units, we hypothesise that populations of net-winged midge should exhibit a high degree of population sub-structuring. 2. Sequence variation in the cytochrome c oxidase subunit I (COI) region of the mitochondrial DNA (mtDNA) was examined to determine patterns of genetic variation and infer historical and contemporary processes important in the genetic structuring of populations of Elporia barnardi. The DNA variation was examined at sites within streams, between streams in the same range, and between mountain ranges in the south-western Cape of South Africa. 3. Twenty-five haplotypes, 641 bp in length, were identified from the 93 individuals sampled. A neighbour-joining tree revealed two highly divergent clades (∼5%) corresponding to populations from the two mountain ranges. A number of monophyletic groups were identified within each clade, associated with individual catchment units. 4. The distribution of genetic variation was examined using analysis of molecular variance (amova). This showed most of the variation to be distributed among the two ranges (∼80%), with a small percentage (∼15%) distributed among streams within each range. Similarly, variation among streams on Table Mountain was primarily distributed among catchment units (86%). A Mantel's test revealed a significant relationship between genetic differentiation and geographical distance, suggesting isolation by distance (P < 0.001). 5. Levels of sequence divergence between the two major clades, representing the two mountain ranges, are comparable with those of some intra-generic species comparisons. Vicariant events, such as the isolation of the Peninsula mountain chain and Table Mountain, may have been important in the evolution of what is now a highly endemic fauna. 6. The monophyletic nature of the catchment units suggests that dispersal is confined to the stream environment and that mountain ridges provide effective physical barriers to dispersal of E. barnardi.
Blood flow restriction resistance training in a recreationally active person with Parkinson’s disease

ABSTRACT Background: Blood flow restriction (BFR) applied during low intensity resistance training (LIRT) exercise produces hypertrophy and strength gains equivalent to traditional training. The effectiveness of BFR-LIRT on persons with Parkinson Disease (PD) has not been investigated. Objective: To determine the effects of BFR-LIRT on a recreationally active person with PD in regards to function, strength, Restless Leg Syndrome (RLS) and safety. Methods: A single subject, A-B-A design was utilized. Each phase lasted 6 weeks. Outcome measures included: 30-second sit-to-stand; Timed Up and Go (TUG); RLS Questionnaire; 3-RM of Cybex Leg Press (LP); Leg Curl (LC); and Leg Extension (LE) measured every 3 weeks for 18 weeks. The intervention phase (B, weeks 6–12) included four lower extremity resistance exercises (LP, LC, LE, calf presses on the LP) with the addition of BFR. The two standard deviation band method was used to determine significance. Results: All outcome measures except the TUG improved significantly by the end of intervention phase. Conclusion: The combination of BFR with LIRT safely lead to an increase in lower extremity strength and function in a person with PD, while decreasing their RLS (improvement from moderate to mild symptoms) resulting in a better quality of life for the participant.
Increased soil release of greenhouse gases shrinks terrestrial carbon uptake enhancement under warming

Warming can accelerate the decomposition of soil organic matter and stimulate the release of soil greenhouse gases (GHGs), but to what extent soil release of methane (CH4) and nitrous oxide (N2O) may contribute to soil C loss for driving climate change under warming remains unresolved. By synthesizing 1,845 measurements from 164 peer‐reviewed publications, we show that around 1.5°C (1.16–2.01°C) of experimental warming significantly stimulates soil respiration by 12.9%, N2O emissions by 35.2%, CH4 emissions by 23.4% from rice paddies, and by 37.5% from natural wetlands. Rising temperature increases CH4 uptake of upland soils by 13.8%. Warming‐enhanced emission of soil CH4 and N2O corresponds to an overall source strength of 1.19, 1.84, and 3.12 Pg CO2‐equivalent/year under 1°C, 1.5°C, and 2°C warming scenarios, respectively, interacting with soil C loss of 1.60 Pg CO2/year in terms of contribution to climate change. The warming‐induced rise in soil CH4 and N2O emissions (1.84 Pg CO2‐equivalent/year) could reduce mitigation potential of terrestrial net ecosystem production by 8.3% (NEP, 22.25 Pg CO2/year) under warming. Soil respiration and CH4 release are intensified following the mean warming threshold of 1.5°C scenario, as compared to soil CH4 uptake and N2O release with a reduced and less positive response, respectively. Soil C loss increases to a larger extent under soil warming than under canopy air warming. Warming‐raised emission of soil GHG increases with the intensity of temperature rise but decreases with the extension of experimental duration. This synthesis takes the lead to quantify the ecosystem C and N cycling in response to warming and advances our capacity to predict terrestrial feedback to climate change under projected warming scenarios.
Protective effects of extracts of lichen Dirinaria consimilis (Stirton) D.D. Awasthi in bifenthrin- and diazinon-induced oxidative stress in rat erythrocytes in vitro

Abstract The intoxication of insecticides such as bifenthrin and diazinon has been reported to generate free radicals, and thereby alter the antioxidant defense system in erythrocytes. The present study is aimed to investigate the protective effects of acetone (DA) and methanolic (DM) extracts of lichen Dirinaria consimilis against bifenthrin and diazinon toxicity in rats’ erythrocytes in vitro. Rats’ erythrocytes were exposed to bifenthrin and diazinon, individually and also in combination with DA or DM at 1 ppm for 3 h at 37 ˚C. By using spectrophotometric methods, all the samples were estimated for changes in hemoglobin (Hb) concentration, malondialdehyde (MDA) levels, and enzyme [Superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferases (GST)] activities. The outcomes showed that both the insecticides were highly toxic to rats’ erythrocytes. Among all groups, both the insecticides and DA exposed groups showed very low levels of MDA content, and GST activity in rats’ erythrocytes, when compared to the control. Besides, DA groups pretreated with both insecticides showed significant improvement of total Hb concentration, SOD, and CAT activities, with respect to the control. Hence, the present results indicate that the extracts of D. consimilis act as an antioxidant agent that reduces oxidative stress burden in insecticides toxicity.
Influence of Resistant and Susceptible Cultivar Blends of Hard Red Spring Wheat on Wheat Stem Sawfly (Hymenoptera: Cephidae) Damage and Wheat Quality Parameters

Use of resistant and susceptible cultivar blends of hard red spring wheat ( Triticum aestivum L.) were investigated over a 3-yr period as a method to suppress wheat stem sawfly, Cephus cinctus Norton, damage. Blends provided inconsistent protection against stem cutting. Differences in estimated harvestable yield were only detected in 1 year. Total yield and milling and baking qualities of blends were not significantly different from those of monocultures. Blends may be useful in situations where the potential for damage by wheat stem sawfly is low to moderate.
Trophic interactions between the Patagonian toothfish, its fishery, and seals and seabirds around Macquarie Island

Macquarie Island is a small subantarctic island that supports a variety of breeding seabird and marine mammal populations. A fishery targeting the Patagonian toothfish Dissostichus elegenoides was established around the island in 1994. For ecological sustainable development (ESD) of the fishery, this study investigated the trophic interactions based on diet composition and annual consumption between Patagonian toothfish, its fishery, and seals and seabirds within the Macquarie Island Exclusive Economic Zone (MI-EEZ). Annual consumption rates for each predator were estimated from dietary data (mostly published sources), energetic budgets, prey energy con- tent, and population size. Results indicated little predation on toothfish by seals or seabirds, or prey competition between toothfish and other marine predators. The greatest dietary overlap with tooth- fish was with gentoo penguins (21% dietary overlap) and southern elephant seals (19%). These over- laps in diet were small relative to those among fur seals (3 species, ≥ 90%), giant petrels (84%), royal and rockhopper penguins (65%), and king and royal penguins and fur seals (>60%). The total annual prey biomass consumed by seabirds, seals, toothfish and the fishery within the MI-EEZ was esti- mated to be 419 774 t, with the greatest consumption in January, at 2779 t d -1 . Pelagic fish (61%, mostly myctophids), followed by pelagic crustaceans (28%, mostly euphausids) and cephalopods (7%) were the major prey. Most prey biomass was consumed by penguins (88%), with comparatively small amounts by toothfish (8%), seals (3%) other seabirds (<1%) and the fishery (0.1%). These results indicate weak trophic linkages between the toothfish, its fishery, and seabirds and seals around Macquarie Island.
Is your health malleable or fixed? The influence of implicit theories on health-related attitudes and behaviour

Abstract Objective: Implicit theories of health describe the extent to which health is perceived as a fixed (entity theory) versus malleable (incremental theory) characteristic. In four studies, it was investigated how these theories correspond to health-related attitudes and behaviours. Design: In Study 1 (N = 130), the relationship of implicit theories of health and health-related behaviours was assessed via self-reports. To investigate their causal influence on health-related attitudes (Study 2; N = 357) and hypothetical food choices (Study 3; N = 351), implicit theories of health were manipulated using fictitious newspaper articles. In Study 4 (N = 235), the relationship of implicit theories and health behaviours in daily life was investigated using experience sampling. Results: Study 1 showed that a stronger incremental theory is positively associated with health behaviours like eating healthily or engaging in physical activity. Studies 2 and 3 revealed that a manipulation of implicit theories of health changes health-related attitudes and hypothetical food choices via an internal health locus of control. Study 4 showed that individuals with a stronger incremental theory reported more health-promoting behaviours in daily life. Conclusion: These findings extend the knowledge about implicit theories as they show that they are highly relevant for health promotion.
Default network interactivity during mentalizing about known others is modulated by age and social closeness

Abstract In young adults, mentalizing about known others engages the default network, with differential brain response modulated by social closeness. While the functional integrity of the default network changes with age, few studies have investigated how these changes impact the representation of known others, across levels of closeness. Young (N = 29, 16 females) and older (N = 27, 12 females) adults underwent functional magnetic resonance imaging (fMRI) scanning while making trait judgments for social others varying in closeness. Multivariate analyses (partial least squares) identified default network activation for trait judgments across both age cohorts. For young adults, romantic partner and self-judgments differed from other levels of social closeness and were associated with activity in default and salience networks. In contrast, default network interactivity was not modulated by social closeness for older adults. In two functional connectivity analyses, both age groups demonstrated connectivity between dorsal and ventral medial prefrontal cortex and other default network regions during trait judgments. However older, but not young, adults also showed increased functional coupling between medial and lateral prefrontal brain regions that did not vary by category of known other. Mentalizing about others engages default and frontal brain regions in older adulthood, and this coupling is poorly modulated by social closeness.