Miracle-dz/UniFAR-FTUs huggingface dataset:
Effet de ligands de G-quadruplexes sur la séquence terminale des télomères

Les telomeres sont des complexes nucleoproteiques situes a lʼextremite des chromosomes. Le role des telomeres dans la protection du genome, et leur implication dans la senescence et le cancer en font une cible privilegiee dans la lutte contre le cancer. Une strategie originale consiste a bloquer lʼacces de lʼextremite telomerique aux proteines necessaires a sa replication et a sa stabilite comme par exemple la telomerase qui est activee dans 85% des cancers ou les proteines POT1 et TRF2 qui protegent lʼextremite telomerique. A cause de la repetition en guanines du brin G, lʼextremite des telomeres peut former des structures en quadruplexes de guanines (G- quadruplexes) dont la stabilisation par des ligands specifiques (Ligands G4) bloque la replication des telomeres et altere son integrite. Le traitement de cellules tumorales par des ligands G4 provoque une dysfonction telomerique associee a une dissociation des proteines POT1 et TRF2 et conduit a une apoptose ou une senescence des cellules. Lʼanalyse de la sequence terminale de lʼextremite telomerique par la methode STELA (Single Telomere Length Analysis) montre que la sequence terminale du brin C se termine majoritairement par la sequence ATC-5ʼ. La proteine POT1 est responsable de la resection du brin C des telomeres en recrutant une exo- nuclease capable de creer le substrat simple-brin pour la telomerase. La depletion de POT1 par ARN interference provoque une deregulation de la terminaison du brin C. Nous avons etudie au cours de notre travail lʼeffet de ligands G4 sur la sequence terminale du brin C telomerique au niveau des telomeres du chromosome XpYp et au niveau de lʼensemble des telomeres en utilisant une modification de la technique de STELA. Nos resultats montrent quʼune faible concentration des derives de la serie des pyridines dicarboxamides provoque un effet mineur mais significatif au niveau de la sequence terminale des telomeres XpYp et au niveau de lʼensemble des telo- meres dans la lignee HT1080. Au contraire, une concentration plus importante ne modifie pas la sequence terminale du brin C dans les cellules HT1080, A549 et HeLa, mais induit un stress replicatif. Lʼeffet modeste de ces ligands sʼexplique par une activite de dissociation incomplete de la proteine POT1 des telomeres comparativement a sa depletion par ARN interference. En effet, nous avons egalement montre que la depletion quasi complete de POT1 par shARN induit une randomisation de la sequence terminale du brin C.
PREDISPOSING FACTORS ASSOCIATED WITH DISEASES IN ANIMALS IN NIGERIA AND POSSIBLE BOTANICAL IMMUNOSTIMULANTS AND IMMUNOMODULATORS: A REVIEW

The factors predisposing to diseases in Nigeria, Sub-Saharan was classified basically as animal, environmental, cliental among others. This review was aimed at broadening the horizon of the Veterinarian. These factors were discussed in the light perspective of the peculiarity of Nigeria being a developing country. The write- up also proffered some solutions using organic botanicals agents which are relatively safe in food animals with little residual effect. Some of the plants suggested are: Allium sativm, Curcuma longa, Discorea japonica, Ziginber officinale, Jatropha gossipofolia, Datura spp and Boerhaavia diffusa. Supplements suggested are ascorbic acid, citric acid and Vitamin E. Immunomodulating supplements suggested are interferons, L-carnitine. The immunomodulating and immunostimulating agents suggested could be used in immunocompromised animals due to contemporary predisposing factors to diseases in this geographical region.
WHEAT YIELD PERFORMANCE AS INFLUENCED BY DEFICIT IRRIGATION

Two field experiments were performed in 2010-2011 and 2011-2012 winter seasons at two sites at Agricultural Experimental Station (AES) in Giza and at Wadi El-Natron Experimental Station (WES), Faculty of Agriculture, Cairo University for studying the effect of three water deficit levels [10%, 20% and 30% (ETc) Crop Evapotranspiration] at different growth stages of wheat (vegetative, flowering, repining, mature stage and all growth stages combined), crop response factor to deficit irrigation (Ky) and impact of deficit on water productivity. The reduction percentage of seed-wheat yield was 13.6, 10.2, 7.2, 3.7 and 3.6% when deficit irrigation was applied in (AES) at all growth stages combined, flowering, repining, vegetative and mature stage respectively. Whereas, the reduction percentage was 14.1, 12.2, 8.2, 12.2 and 5.7% for the same treatments in (WES). The yield response factor (Ky) values increased by increasing the deficit level. Crop response to deficit irrigation (ky) according to the different stressed-growth stages is found in the following order flowering> repining> mature> overall season> vegetative in both sits under studied conditions.
RESPONSE OF ONION TO DIFFERENT LEVELS OF IRRIGATION WATER AND FERTILIZATION: (II) BIOMASS, YIELD, SIZE CLASSES, QUALITY, AND WATER USE

An experiment was conducted to study the effect of different levels of irrigation water and NPK fertilizers on bulb yield and quality of onion (Allium cepa L.). Four levels of irrigation (65, 75, 85, and 100% of ETc) and four rates of NPK fertilizers (0, 50, 75, and 100% of the applied recommended rate of NPK) were arranged in a randomized complete block design, replicated three times. Onion yield, marketable yield, dry bulb yield, total dry biomass, WUEy of total yield, WUEm of marketable yield, WUEd of dry yield, TSS, N, P, and K were determined under trickle irrigation on a clay loam soil in 2011 and 2012. High yields were observed for the 100 and 85 % ETc with 100 and 75 % NPK treatments. The increase in applied water level significantly decreased the percentage of dry matter and total dry biomass in the onion bulb and the increase in NPK rates increased significantly the percentage of dry matter in the onion bulb in both years. There were no significant effect on WUEy and WUEm when decreasing the water level from 100% ETc to 85 % ETc and decreasing the NPK rate from 100 % to 75 %. The interaction between applied water levels and NPK application rates showed that the maximum significant increase in TSS, N, P, and K was with low water levels and higher levels of NPK rates in both 2011 and 2012 seasons.
Expression of the long noncoding MALAT-1 RNA in human lung cancer tissues and its clinical significance

Objective To study the relationship between lung cancer clinicopathological features and MALAT-1 lncRNA expression in human lung cancer tissues.Methods The MALAT-1 expression levels in the cancerous and normal tissues of 76 lung cancer patients were measured by real-time quantitative PCR (RT-qPCR). Results The expression levels of MALAT-1 gene were down-regulated in 58％ (44/76) primary lung cancer patients,compared with the matched normal tissues.The difference of MALAT-1 expression level between cancerous tissues and the matched normal tissues was significant (t =-7.648,P ＜0.001). Furthermore,The expression levels of MALAT-1 had difference among cell differentiation,tumor metastasis,tumor pathological classification and smoking (P ＜ 0.05). The survival time of the group which had ＜ 50％ decreased MALAT-1 expression level was significantly longer than that whose MALAT-1 expression level were reduced ≥50％. Cox multivariate regression analysis showed that cell differentiation,tumor metastasis,tumor pathological classification amd expression of MALAT-1 were an independent prognostic factor for NSCLC.Conclusions The expression of MALAT-1 might play an important role in non-small lung cancer and thus maybe a biomarker for lung cancer in screening,diagnosis and prognosis. Key words: Lung cancer; MALAT-1 ; lncRNA; Metastasis; Prognosis
Time-Varying Stock Return Predictability: The Eurozone Case

In this paper, we test the existence of predictability in eleven Eurozone stock markets, using both regressions with constant coefficients and with time-varying coefficients. Our results show that there is statistical evidence of predictability in some countries. The economic value of the forecasting models is much stronger than what could be inferred, based on the statistical tests. A meanvariance investor could have obtained substantial utility gains in most countries. Overall, models with time-varying parameters perform slightly better than models with constant coefficients.
Amino acid digestibility in plant protein sources fed to growing pigs

Objective The objective was to determine standardized ileal digestibility (SID) of amino acids (AA) in 11 plant protein sources fed to growing pigs. Methods Eleven feed ingredients used were sesame meal, two sources of soybean meal (SBM) produced in the Republic of Korea, a source of SBM produced in India, high-protein distillers dried grains (HPDDG), perilla meal, canola meal, copra meal, corn germ meal, palm kernel expeller, and tapioca distillers dried grains (TDDG). Experimental diets were prepared to contain each test ingredient as a sole source of AA, and a nitrogen-free diet was also prepared to estimate the basal ileal endogenous losses of AA. Twelve barrows surgically fitted with T-cannulas at the distal ileum with an initial body weight of 29.0 kg (standard deviation = 3.0) were individually housed in metabolism crates equipped with a feeder and a nipple drinker. A 12×9 incomplete Latin square design was employed with 12 experimental diets, 12 animals, and 9 periods. After a 5-d adaptation period, ileal digesta were collected on d 6 and 7 in each experimental period. Results Values for apparent ileal digestibility of most indispensable AA in three sources of SBM were greater compared with other test ingredients except HPDDG and canola meal (p<0.05). Pigs fed diets containing SBM sources had also greater SID of most indispensable AA compared with those fed diets containing other test ingredients (p<0.05) except for HPDDG and canola meal. There was no difference in the apparent ileal digestibility and SID of AA among sources of SBM. The TDDG had the least value for the SID of methionine among test ingredients (p<0.05). Conclusion The SID of most AA in SBM, HPDDG, and canola meal were greater than those in sesame meal, perilla meal, copra meal, and TDDG.
Hubungan Religiusitas dan Kontrol Diri dengan Perilaku Seksual Pranikah Remaja di MAN 1 Samarinda

Perilaku seksual pranikah di kalangan remaja meningkat dan banyak yang berdampak negatif pada perkembangan psikologis remaja. Penelitian ini bertujuan untuk mengetahui hubungan antara religiusitas dan kontrol diri dengan perilaku seksual remaja. Penelitian terdiri dari satu variabel dependen yaitu perilaku seksual pranikah dan dua variabel bebas yaitu religiusitas dan kontrol diri. Data dikumpulkan dengan menggunakan kuesioner. Subjek dalam penelitian ini adalah siswa MAN 1 Samarinda, yang berjumlah 95 siswa. Teknik analisis data yang digunakan adalah analisis regresi berganda. Hasil penelitian menunjukkan ada hubungan negatif dan signifikan antara religiusitas dan perilaku seksual pranikah dengan nilai beta = -0235, t = -2170 dan p = 0,033, dan ada juga hubungan negatif dan signifikan antara kontrol diri dengan seksual pranikah. Perilaku dengan nilai beta = 0,221, t = 2,042, dan p = 0,044. Hasil penelitian juga menunjukkan bahwa ada hubungan yang signifikan antara religiusitas dan kontrol diri dengan perilaku seksual pranikah dengan nilai F = 3,251, R2 = 0,066, dan p = 0,043
Comparative protein profiling of milk of Nili-Ravi buffaloes, Sahiwal and cross bred cows by SDS-PAGE.

Received: Revised: Accepted: Published online: June 21, 2016 August 27, 2016 September 08, 2016 November 17, 2016 Milk of different species may vary in protein quality and quantity. A proteomic approach for high-resolution analyses of the complex mixture of milk proteins by SDS polyacrylamide gel electrophoresis (PAGE) is of great importance due to its exceptional resolving capacity. Degreased milk samples (non-infected) of Nili-Ravi buffaloes, Sahiwal and cross-bred cows were used for comparative protein profiling using 12% SDS polyacrylamide gels. Protein profiling clearly resolved the major milk peptides. Qualitative differences were observed in high, medium and low molecular weight (MW) zones. In high molecular weight (M. wt.) zone, proteins of ~208 kDa and ~190 kDa were detected in all tested samples. In medium M. wt. zone, three peptides i.e. lactoferrin (78.2kDa), serum albumen (66.2kDa) and heavy chain of immunoglobulin (IgG) (54 kDa) were detected in all samples while a prominent band of ovalbumen (45kDa) was also detected mainly in cow milk samples. In low M. wt. zone, clear bands of milk caseins were detected. All four casein (CN) bands i.e. αS2 – CN (29 kDa), αS1 – CN (27 kDa), β CN (24 kDa) and κCN (22 kDa) were detected in cross-bred and Sahiwal cows. However, in milk of Nili-Ravi buffaloes, three casein protein i.e. αS2 – CN (29 kDa), β CN (25 kDa) and κCN (22 kDa) were detected. In milk of Nili-Ravi buffaloes, αS1 – CN (27 kDa) was not detected. Moreover, a band of β-lactoglobulin (~18 kDa) was detected in milk of cross-bred cows and not in other samples especially Nili-Ravi buffaloes. As the αS1-casein and β-lactoglobulin are the major allergens, milk of Nili-Ravi buffaloes that lacks these peptides can be used for development of non-allergic or hypoallergenic dairy products. Deferential peptides may also help to distinguish the milk from different tested breeds. ©2016 PVJ. All rights reserved
Forest managment effect in diversity and tree composition of a temperate forest in northwestern Mexico

In the present study, the effect of silvicultural practices in diversity and composition of tree species in a temperate forest in northwestern Mexico was evaluated. Forty six plots were evaluated every 10 years (1986, 1996 and 2006). Indices of alpha and beta diversity of the community of trees, abundance, dominance, frequency and importance value index (IVI) of each species were estimated. The results show that logging changes the diversity and composition of the tree strata. The tree community maintains the number of species, but decreases the Margalef and Shannon-Wiener indices of alpha diversity, and composition was changed in 16 % (beta diversity). Pinus increases its relative abundance, dominance, frequency and IVI, meanwhile Quercus declines. Pinus arizonica and P. durangensis are the species of greater ecological importance.
EFFECT OF FOREST COVER ON ECTOPARASITES OF SMALL MAMMALS IN THE BRAZILIAN ATLANTIC FOREST

Habitat reduction is one of the greatest threats to biodiversity and one of its many expected consequences is the negative impacts on host species. In this study, we evaluated if the reduction in the forest cover positively affected the richness and abundance of ectoparasites and rates of parasitism on mammal species. The study was carried out in the Atlantic Forest biome in the state of Bahia between January to March and September to November of 2011. We sampled four areas with different amounts of forest cover (%) in the municipalities of Valenca (25%), Nilo Pecanha (35%), Camamu (45%) and Jaguaripe (55%). We identified twenty species of ectoparasites (fleas, mites, ticks) on 130 small mammals from 18 species (14 rodents and four marsupials), and the most abundant ectoparasites were mites. We confirmed our main hypothesis: the greater richness and abundance of ectoparasites was associated with a reduction in forest cover, with possible epidemiological implications. The prevalence of ectoparasitism per area varied from 91% in Valenca to 50% in Jaguaripe. The mean intensity of ectoparasites per host was 6.5 for Valenca, 6.4 for Nilo Pecanha, 10.4 for Camamu and 3.2 for Jaguaripe. Ours findings confirm that the reduction in the amount of forested is related to amplified rates of parasitism and changes in ectoparasites abundance and species richness.
Pyrolysis of Cigarette Ingredients Labelled with Stable Isotopes

Abstract It is important to know how tobacco additives behave when cigarettes are smoked, whether they transfer intact to the smoke or whether there is any decomposition during smoking. Pyrolysis-GC-MS is a technique that can be focussed upon the effects of combustion from a single material free from interference from the complex mixture of different components present in the smoke. However, because pyrolysis is a model technique, the results need to be validated by comparison with cigarette smoke chemistry. In a previous paper we presented such a method for modelling the smoke chemistry from a burning cigarette using pyrolysis-GC-MS. The transfer and the extent of degradation of anisole, p-anisaldehyde, benzaldehyde, isoamylisovalerate, methyl trans-cinnamate and vanillin within a burning cigarette were estimated using this pyrolysis method. When these data were compared with results from smoke studies from 14C-analogues of the materials, the high levels of transfer predicted by pyrolysis were found to be generally consistent with the smoke chemistry data. However, there were still two outstanding issues. Firstly, there was some ambiguity in the labelled study about whether vanillin actually transferred without degradation or not. Furthermore, the results from the 14C-labelled study showed a greater extent of degradation for p-anisaldehyde than that indicated from the pyrolysis experiments. The purpose of the current study was to present some new information obtained to address these questions by better understanding the effect upon the smoke chemistry from adding vanillin and p-anisaldehyde, and the relationship between the smoke chemistry and the pyrolysis results. Components were identified in the smoke from cigarettes loaded with p-anisaldehyde and vanillin labelled with 18O and 13C. The extent of degradation from each additive was estimated by identifying labelled degradation products in the smoke. Because there was a clear distinction between the mass spectra from the labelled compounds and their natural counterparts, the major degradation products from the labelled compounds could be readily identified in the chromatograms of the corresponding smoke extracts. Evidence of some degradation at above the transfer temperature was indicated for both additives. The amount of degradation was found to be less than 1% for p-anisaldehyde and only 0.1% for vanillin. This low level of degradation was acceptably consistent with the intact transfer values of 97% and 100%, respectively, obtained by pyrolysis.
Total phenolic contents and antioxidant activity profile of selected cereal sprouts and grasses

ABSTRACT Quantitative profiling of total phenolic content (TPC) and antioxidant activity (AOA) in seeds, sprouts, and grasses of corn (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.) were performed. TPC and AOA were strongly correlated and increased with days. Grasses between 10 and 13 days showed the highest TPCs and AOA. The AOA in sprouts and grasses were a fifth and two-thirds of ascorbic acid (AA) standard, respectively, which are higher than in several popular natural antioxidants. Further cell-based studies or in vivo studies could be considered in future to draw more valuable information related to human health.
Mycorrhizal Fungi Enhance Resistance to Herbivores in Tomato Plants with Reduced Jasmonic Acid Production

Arbuscular mycorrhizal (AM) fungi favor plant growth by improving nutrient acquisition, but also by increasing their resistance against abiotic and biotic stressors, including herbivory. Mechanisms of AM fungal mediated increased resistance include a direct effect of AM fungi on plant vigor, but also a manipulation of the hormonal cascades, such as the systemic activation of jasmonic acid (JA) dependent defenses. However, how AM fungal inoculation and variation in the endogenous JA production interact to produce increased resistance against insect herbivores remains to be further elucidated. To address this question, three genotypes of Solanum lycopersicum L. , a JA-biosynthesis deficient mutant, a JA over-accumulating mutant, and their wild-type were either inoculated with AM fungi or left un-inoculated. Plant growth-related traits and resistance against Spodoptera littoralis (Boisduval) caterpillars, a major crop pest, were measured. Overall, we found that deficiency in JA production reduced plant development and were the least resistant against S. littoralis. Moreover, AM fungi increased plant resistance against S. littoralis, but such beneficial effect was more pronounced in JA-deficient plant than on JA over-accumulating plants. These results highlight that AM fungi-driven increased plant resistance is negatively affected by the ability of plants to produce JA and that AM fungi complement JA-mediated endogenous plant defenses in this system.
Yield of Tifton 85 grass under irrigation and nitrogen doses.

The study was conducted on a dairy farm in the municipality of Xambre, Northwest region of Parana, in the period from March 2011 to February 2012 to evaluate the yield and botanical composition of Tifton 85 with and without irrigation under nitrogen doses. The experimental plots were implanted in completely randomized blocks with and without irrigation and subplots through four nitrogen levels: 0, 20, 40 and 60 kg ha-1 per grazing cycle, with four replications. The yield in dry matter (DM) was higher under irrigation, responding linearly to increasing nitrogen fertilization. At a dose of 60 kg N ha-1 yields of 39279 and 27826 kg DM ha-1 were obtained, with and without irrigation, respectively. The leaf stem ratio was not affected by irrigation. The overall mean percentage of dead material with and without irrigation was equal to 13 and 17%, respectively.
EFFECT OF IRRIGATION INTERVALS AND FOLIAR SPRAYING TREATMENTS ON GROWTH AND YIELD OF SOME RICE CULTIVARS

The field experiments were carried out at a Farm in El-Abhar Village, El-Hamoul Center, Kafr El-Sheikh Governorate, during 2011 and 2012 seasons to determine the effect of irrigation intervals (irrigation every 2, 4 and 8 days) and foliar spraying treatments (without foliar spraying, foliar spraying with Flowering-Spring fertilizer, Tecamin-Max fertilizer, Potassium- Sulphate and Ascorbic-Acid) on growth, yield and its attributes of some rice cultivars (IET 1444, Giza 177 and Giza 178). Each irrigation intervals was performed in separate experiment. Every experiment of irrigation intervals was carried out in strip plot design with three replications. The results showed that normal irrigation schedule every 2 days (control treatment) associated with significant increases in all studied characters and resulted in the highest values in both seasons. IET 1444 cultivar significantly superior the two local cultivars Giza 177 and Giza 178 and resulted in the highest values of most studied characters. Foliar spraying rice plants with Tecamin max fertilizer surpassed other studied foliar spraying treatments and resulted in the highest values of all studied characters in both seasons. This treatment followed by spraying with Flowering spring fertilizer without significant differences between them in most characters, then potassium sulphate and then ascorbic acid in both seasons. It can be concluded that, irrigated IET 1444 rice cultivar every 6 days and foliar spayed with Tecamin max or Flowering spring fertilizers could be recommend to raise productivity and save irrigation water and irrigated Giza 178 and Giza 177 rice cultivars every 2 days and foliar spayed with Tecamin max or Flowering spring fertilizers to maximize productivity under the environmental conditions of El-Hamoul district, Kafr El-Sheikh Governorate.