Miracle-dz/UniFAR-FTUs huggingface dataset:
Fitness of mCherry Reporter Tick-Borne Encephalitis Virus in Tick Experimental Models

The tick-borne encephalitis virus (TBEV) causes a most important viral life-threatening illness transmitted by ticks. The interactions between the virus and ticks are largely unexplored, indicating a lack of experimental tools and systematic studies. One such tool is recombinant reporter TBEV, offering antibody-free visualization to facilitate studies of transmission and interactions between a tick vector and a virus. In this paper, we utilized a recently developed recombinant TBEV expressing the reporter gene mCherry to study its fitness in various tick-derived in vitro cell cultures and live unfed nymphal Ixodes ricinus ticks. The reporter virus was successfully replicated in tick cell lines and live ticks as confirmed by the plaque assay and the mCherry-specific polymerase chain reaction (PCR). Although a strong mCherry signal determined by fluorescence microscopy was detected in several tick cell lines, the fluorescence of the reporter was not observed in the live ticks, corroborated also by immunoblotting. Our data indicate that the mCherry reporter TBEV might be an excellent tool for studying TBEV-tick interactions using a tick in vitro model. However, physiological attributes of a live tick, likely contributing to the inactivity of the reporter, warrant further development of reporter-tagged viruses to study TBEV in ticks in vivo.
The provisional DENIS I-band catalog of galaxies revisited

We present an extended and improved version of the provisional I -band catalog of DENIS galaxies. Galaxies not cross-identified with already known galaxies have been visually checked using the DSS1 images and, occasionnally, the coordinates were remeasured. The typical accuracy of coordinates is about $2''$. The typical error on I -band magnitude is about 0.2 mag. The final catalog gives the most common names, the J2000 coordinates, the I -band magnitude, the diameter, the axis ratio and the position angle for about $43\,000$ galaxies. It covers roughly 67% of the southern hemisphere. It is homogeneously sampled up to $I=$ 14.5 mag. It is available through the Centre de Donnees de Strasbourg (CDS). The data are also available via the LEDA database.
Does Pornography Misinform Consumers? The Association between Pornography Use and Porn-Congruent Sexual Health Beliefs

Pornography may contribute to sexual health illiteracy due to its often fantastical and unrealistic depictions of sex. This cross-sectional study investigated whether pornography use was associated with holding porn-congruent sexual health beliefs among a sample of 276 Australian and Singaporean university students (Mage = 23.03, SDage = 7.06, 67.9% female, 47.8% Australian). The majority of participants (95.5% of males and 58.9% of females) reported viewing pornography in the past six months. Perceived realism of pornography and prior sexual experience were tested as potential moderators of the relationship between pornography use frequency and sexual health beliefs. Pornography use frequency showed no zero-order association with sexual health beliefs in the overall sample (although a significant zero-order association was observed among female participants). However, a significant positive association between porn use and porn-congruent sexual health beliefs was found in the overall sample, after controlling for demographic variables. Neither perceived realism nor sexual experience were found to act as moderators. Interestingly, prior sexual experience showed a significant zero-order association with sexual health beliefs, such that prior sexual experience was associated with holding porn-congruent beliefs. Perceived realism was unrelated to porn-congruent sexual health beliefs. The study provides some preliminary support for pornography having a misinformation effect on the sexual health knowledge of consumers.
9p21 Locus Polymorphism Is A Strong Predictor of Metabolic Syndrome and Cardiometabolic Risk Phenotypes Regardless of Coronary Heart Disease

The world population is genetically predisposed to metabolic syndrome (MetS) and its components, also known as cardiometabolic risk phenotypes, which can cause severe health complications including coronary heart disease (CHD). Genetic variants in the 9p21 locus have been associated with CHD in a number of populations including Pakistan. However, the role of the 9p21 locus in MetS and cardiometabolic risk phenotypes (such as obesity, hypertension, hyperglycemia, and dyslipidemia) in populations with CHD or no established CHD has not been explored. Therefore, the present study was designed to explore the association of the minor/risk allele (C) of 9p21 locus SNP rs1333049 with MetS or its risk phenotypes regardless of an established CHD, in Pakistani subjects. Genotyping of rs1333049 (G/C) was performed on subjects under a case-control study design; healthy controls and cases, MetS with CHD (MetS-CHD+) and MetS with no CHD (MetS-CHD−), respectively. Genotype and allele frequencies were calculated in all study groups. Anthropometric and clinical variables (Means ± SD) were compared among study groups (i.e., controls, MetS + CHD and MetS-CHD) and minor/risk C allele carriers (GC + CC) vs. non-carriers (Normal GG genotype). Associations of the risk allele of rs1333049 SNP with disease and individual metabolic risk components were explored using adjusted multivariate logistic regression models (OR at 95% CI) with a threshold p-value of ≤0.05. Our results have shown that the minor allele frequency (MAF) was significantly high in the MAF cases (combined = 0.63, MetS-CHD+ = 0.57 and MetS-CHD− = 0.57) compared with controls (MAF = 0.39). The rs1333049 SNP significantly increased the risk of MetS, irrespective of CHD (MetS-CHD+ OR = 2.36, p < 0.05 and MetS-CHD− OR = 4.04, p < 0.05), and cardiometabolic risk phenotypes; general obesity, central obesity, hypertension, and dyslipidemia (OR = 1.56–3.25, p < 0.05) except hyperglycemia, which lacked any significant association (OR = 0.19, p = 0.29) in the present study group. The 9p21 genetic locus/rs1333049 SNP is strongly associated with, and can be a genetic predictor of, MetS and cardiometabolic risks, irrespective of cardiovascular diseases in the Pakistani population.
Cation Exchange Resins for Predicting Available K on K-Deficient Soils: Extraction Capacity among Different Soil K Pools and First Insights on the Contribution of K Solubilized by Rhizosphere Microbes

The contribution of non-exchangeable soil K in the total K availability may be essential to K deficient soils; however, less attention has been paid so far for proposing soil tests that assess the bioavailable structural K that is solubilized by soil microbes during a growing season. The objectives of this study were to evaluate cation exchange resins for their performance in predicting K uptake by plants; to investigate the relationships between rhizosphere soil microbes, different soil K pools, and soil parameters; and to assess their exchange capacity with respect to K derived by feldspars. A pot experiment with winter wheat was conducted on K deficient soils, several soil tests were examined, and rhizosphere K solubilizing microbial population was assessed. Single and multiple regressions showed that cation resins performed better in predicting total plant K uptake than the other chemical extractants (r2 = 0.64, r2 = 0.85, respectively, p ≤ 0.001), whereas the PCA analysis and Pearson correlation tests revealed a positive correlation between K derived by feldspars, K uptake, and the K solubilizing rhizosphere microbial population. The above was further confirmed by the mapping of cation resins of extraction capacity, which showed a significant contribution of K derived by feldspars (15.6%).
Evaluating Pre-Trained Transformers on Italian Administrative Texts

In recent years, Transformer-based models have been widely used in NLP for various downstream tasks and in different domains. However, a language model explicitly built for the Italian administrative language is still lacking. Therefore, in this paper, we decided to compare the performance of five different Transformer models, pre-trained on general purpose texts, on two main tasks in the Italian administrative domain: Name Entity Recognition and multi-label document classification on Public Administration (PA) documents. We evaluate the performance of each model on both tasks to identify the best model in this particular domain. We also discuss the effect of model size and pre-training data on the performances on domain data. Our evaluation identifies UmBERTo as the best-performing model, with an accuracy of 0.71, an F1 score of 0.89 for multi-label document classification, and an F1 score of 0.87 for NER-PA.
Involvement of caveolin-1 in skin diseases

The skin is the outermost layer and largest organ in the human body. Since the skin interfaces with the environment, it has a variety of roles, including providing a protective barrier against external factors, regulating body temperature, and retaining water in the body. It is also involved in the immune system, interacting with immune cells residing in the dermis. Caveolin-1 (CAV-1) is essential for caveolae formation and has multiple functions including endocytosis, lipid homeostasis, and signal transduction. CAV-1 is known to interact with a variety of signaling molecules and receptors and may influence cell proliferation and migration. Several skin-related disorders, especially those of the inflammatory or hyperproliferative type such as skin cancers, psoriasis, fibrosis, and wound healing, are reported to be associated with aberrant CAV-1 expression. In this review, we have explored CAV-1 involvement in skin physiology and skin diseases.
Microbial diversity of meat products under spoilage and its controlling approaches

Meat spoilage (MS) is a complex microbial ecological process involving multiple specific microbial interactions. MS is detrimental to people's health and leads to the waste of meat products which caused huge losses during production, storage, transportation, and marketing. A thorough understanding of microorganisms related to MS and their controlling approaches is a necessary prerequisite for delaying the occurrence of MS and developing new methods and strategies for meat product preservation. This mini-review summarizes the diversity of spoilage microorganisms in livestock, poultry, and fish meat, and the approaches to inhibit MS. This would facilitate the targeted development of technologies against MS, to extend meat's shelf life, and effectively diminish food waste and economic losses.
Humoral and cellular immune response and the safety of third SARS-CoV-2 mRNA vaccine with longer interval after the second vaccination in kidney transplant recipients

We evaluated the humoral and cellular immune responses and safety of the third severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccine with a longer interval after the second vaccination in kidney transplant recipients (KTRs). We enrolled 54 kidney transplant recipients without a history of coronavirus disease 2019 (COVID-19), who received a third dose of the vaccine. We assessed anti-SARS-CoV-2 spike antibody and antigen-specific T cells using enzyme-linked immunospot (ELISpot) against the spike protein at baseline, after the second vaccination, and after the third vaccination. We also evaluated the adverse events related to each dose of the vaccine. The duration between the second and third vaccinations was 7 ± 1 month. All 17 (100%) KTRs with anti-SARS-CoV-2 antibody positivity after the second vaccination and 27 of 37 (73%) KTRs without anti-SARS-CoV-2 antibody positivity after the second vaccination were positive for anti-SARS-CoV-2 antibodies (p=0.022). Anti-SARS-CoV-2 antibody titers were significantly higher than those after the second vaccination (p<0.001). Age ≥ 60 years and lymphocyte count < 1150/mm3 were confirmed as risk factors for anti-SARS-CoV-2 antibody negativity after the third vaccination in multivariate regression analysis. ELISpot cytokine activities were positive after the third vaccination in 26 of 29 (90%) KTRs with ELISpot cytokine activity positivity after the second vaccination and 12 of 24 (50%) KTRs without ELISpot cytokine activity after the second vaccination. The rate of change in cytokine activity after the third vaccination was significantly higher than that after the second vaccination (p<0.001). Only lymphocyte counts less than 1150/mm3 were confirmed as risk factors for ELISpot cytokine activity negativity in the multivariate regression analysis. Systemic adverse events classified as greater than moderate did not differ for each vaccine dose. None of the patients showed clinical symptoms of acute rejection. The third SARS-CoV-2 mRNA vaccine administration, with a longer interval after the second vaccination, improved humoral and cellular immune responses to SARS-CoV-2 mRNA vaccines without severe adverse effects in the KTRs.
Comparative analysis of intestinal flora between rare wild red-crowned crane and white-naped crane

Introduction Animal intestines are extremely rich in microbial ecosystems. Numerous studies in different fields, such as epidemiology and histology, have revealed that gut microorganisms considerably mediate the survival and reproduction of animals. However, gut microbiology studies of homogeneously distributed wild cranes are still rare. This study aimed to understand the structural composition of the gut microbial community of wild cranes and elucidate the potential roles of the microorganisms. Methods We used high-throughput sequencing to analyze the gut microbial community structure of wild cranes in the Zhalong Nature Reserve. Results A total of 1,965,683 valid tags and 5248 OTUs were obtained from 32 fecal samples. Twenty-six bacteria phyla and 523 genera were annotated from the intestinal tract of the red-crowned crane. Twenty-five bacteria phyla and 625 genera were annotated from the intestine of the white-naped crane. Firmicutes, Proteobacteria, and Bacteroidetes are the dominant bacterial phyla in the intestinal tract of red-crowned cranes, while Catellicoccus, Lactobacillus, Neisseria, and Streptococcus were the dominant genera. The dominant bacterial phyla in the intestinal tract of white-naped cranes were Firmicutes, Proteobacteria, Bacteroidetes, Epsilonbacteraeota, Actinobacteria, and Fusobacteria. However, the dominant genera were Catellicoccus, Lactobacillus, Neisseria, Campylobacter, Streptococcus, Anaerobiospirillum, Romboutsia, Turicibacter, Haemophilus, and Lautropia. Firmicutes had significantly higher relative abundance in the intestine of the red-crowned than white-naped cranes (P < 0.05). However, the relative abundance of Actinobacteria and Bacteroidetes was significantly higher (P < 0.05) in the intestines of white-naped than red-crowned cranes. The diversity of the intestinal flora between the two crane species was significantly different (P < 0.05). Besides, the alpha diversity of the intestinal flora was higher for white-naped than red-crowned cranes. Eight of the 41 functional pathways differed in the gut of both crane species (P < 0.05). Discussion Both species live in the same area and have similar feeding and behavioral characteristics. Therefore, host differences are possibly the main factors influencing the structural and functional differences in the composition of the gut microbial community. This study provides important reference data for constructing a crane gut microbial assessment system. The findings have implications for studying deeper relationships between crane gut microbes and genetics, nutrition, immunity, and disease.
Caffeine is negatively associated with depression in patients aged 20 and older

Introduction Previous studies have observed the association between caffeine intake and depression, but few have considered the potential threshold effect of this issue. Therefore, the study aimed to examine the association between caffeine consumption and depression in patients aged 20 years or older using curve fitting analysis. Methods The population was 3,263 patients from the 2017 to 2018 National Health and Nutrition Examination Survey (NHANES) with reliable answers to questions of caffeine intake and depression. Participants’ depression levels were assessed using the 9-item Patient Health Questionnaire (PHQ-9) depression scale and the caffeine consumption were investigated in a private room of NHANES. The confounding variables of this study included level of education, monthly sleepiness, age, marital status, race, cigarette smoking, sex and recreational activities. Results In linear regression analysis, patients with a higher PHQ-9 score tend to have less caffeine intake. A similar conclusion was drawn in logistic regression model using PHQ-9 ≥ 10 as a cut-off score for depression. But when caffeine intake exceeded 90 mg, there was no significant association between caffeine intake and depression based on the curve fitting analysis. Discussion These results suggest that people can consume some caffeine to reduce depression. But further study is needed to examine the precise causal relationship between these factors.
Novel organic selenium source hydroxy-selenomethionine counteracts the blood-milk barrier disruption and inflammatory response of mice under heat stress

Heat stress (HS) in summer has caused huge economic losses to animal husbandry production recently. When mammary gland is exposed to high temperatures, it will cause blood-milk barrier damage. Hydroxy-selenomethionine (HMSeBA) is a new selenium source with better guarantee of animals’ production performance under stress, but whether it has protective effect on heat stress-induced blood-milk damage is still unclear. We established mammary epithelial cells and mice heat stress injury models to fill this research gap, and hope to provide theoretical basis for using HMSeBA to alleviate heat stress damage mammary gland. The results showed that (1) Heat stress significantly decreases in vitro transepithelial electrical resistance (TEER) and cell viability (P < 0.01), and significantly decreases clinical score, histological score, and total alveoli area of mice mammary gland tissue (P < 0.01). (2) HMSeBA significantly increases TEER and fluorescein sodium leakage of HS-induced monolayer BMECs (P < 0.01), significantly improves the milk production and total area of alveoli (P < 0.01), and reduces clinical score, histological score, mRNA expression of heat stress-related proteins, and inflammatory cytokines release of heat-stressed mice (P < 0.01). (3) HMSeBA significantly improves tight junction structure damage, and significantly up-regulated the expression of tight junction proteins (ZO-1, claudin 1, and occludin) as well as signal molecules PI3K, AKT, and mTOR (P < 0.01) in heat-stressed mammary tissue. (4) HMSeBA significantly increases glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and superoxide dismutase release (SOD) (P < 0.01) and significantly reduce malondialdehyde (MDA) expression (P < 0.01) in heat-stressed mammary tissue. In conclusion, this study implemented heat-stressed cell and mice model and showed that HMSeBA significantly regulate antioxidant capacity, inhibited inflammation, and regulate tight junction proteins expression in blood-milk barrier via PI3K/AKT/mTOR signaling pathway, so as to alleviate mammary gland damage and ensure its structure and function integrity.
Layered habitats: An evolutionary model for present-day recreational needs

Urbanisation and lifestyle-related illnesses increase globally. This highlights the need to shape modern human habitats to support basic recreational needs, promoting such things as physical activity and restoration of high stress levels and cognitive fatigue. Previous research suggests eight perceived qualities in the outdoor environment, described as eight perceived sensory dimensions, as universally meaningful to people in this regard. However quite extensively studied in relation to various health and wellbeing outcomes, cognitive, human sensitivity and appreciation for these qualities has not yet been explicitly analysed from an evolutionary perspective. This paper investigates their possible evolutionary roots and suggests an order for their development. This is linked with empirical findings on their relative capacity to support restoration of stress and cognitive fatigue. Qualities of earlier origin are suggested to correspond to older, more fundamental adaptations. Each subsequently developed quality implies an increased complexity of our environmental relations, associated with higher demands on more recently developed capacities. The proposed model thus links the more restorative Serene, Sheltered, Natural, and Cohesive perceived sensory dimensions with earlier stages of our development while the more demanding Diverse, Open, Cultural, and Social qualities are associated with more recent transitions. It might be of relevance when shaping modern human habitats from a health-promoting perspective, and have applications in the planning and design of, e.g., health care settings, rehabilitation gardens, urban green areas, recreational forests or other similar outdoor environments.
Targeting H3K27me3 demethylase to inhibit Shh signaling and cholesterol metabolism in medulloblastoma growth

Previously we uncovered the epigenetic regulation of medulloblastoma that low levels of H3K27me3 are required for Shh target gene expression and medulloblastoma growth. Since Jmjd3, an H3K27me3 demethylase, is responsible for maintaining low H3K27me3 at Shh target genes, targeting Jmjd3 could be an efficient way to inhibit Shh signaling and medulloblastoma growth. Here we show that the small molecule GSK-J4, an inhibitor of Jmjd3, significantly inhibited the expression of Shh target genes in Shh responsive cell models and primary cerebellar granule neuron precursors. GSK-J4 also significantly reduced the growth of primary Shh medulloblastoma cultures. Treating human medulloblastoma cell line DaoY by GSK-J4 led to cell cycle arrest at G0/G1 phase with decreased cells in S-phase. Tumor cell proliferation was significantly inhibited by GSK-J4 treatment. Gene expression analyses showed that GSK-J4 additionally constrained the expression of key genes in cholesterol biosynthesis. Our results highlight the possibility that targeting H3K27me3 demethylase Jmjd3 with GSK-J4 to inhibit Shh signaling and cholesterol metabolism is a potential application to treat Shh medulloblastoma.