Miracle-dz/UniFAR-FTUs huggingface dataset:
Responses in a Mouse Model of Infection Systemic and Cerebral Inflammatory Swine and Human Pathogen, Induces Strong Serotype 2, an Important Streptococcus suis

Streptococcus suis , an important swine and human pathogen, causes septic shock and meningitis. The pathogenesis of both systemic and CNS infections caused by S. suis is poorly understood. A hematogenous model of infection in CD1 mice was developed to study the systemic release of cytokines during the septic shock phase and the proinﬂammatory events in the CNS associated with this pathogen. Using a liquid array system, high levels of systemic TNF- (cid:1) , IL-6, IL-12, IFN- (cid:2) , CCL2, CXCL1, and CCL5 were observed 24 h after infection and might be responsible for the sudden death of 20% of animals. Infected mice that survived the early sepsis later developed clinical signs of meningitis and exhibited lesions in the meninges and in numerous regions of the brain, such as the cortex, hippocampus, thalamus, hypothalamus, and corpus callosum. Bacterial Ags were found in association with microglia residing only in the affected zones. In situ hybridization combined with immunocytochemistry showed transcriptional activation of TLR2 and TLR3 as well as CD14, NF- (cid:3) B, IL-1 (cid:4) , CCL2, and TNF- (cid:1) , mainly in myeloid cells located in affected cerebral structures. Early transcriptional activation of TLR2, CD14, and inﬂammatory cytokines in the choroid plexus and cells lining the brain endothelium suggests that these structures are potential entry sites for the bacteria into the CNS. Our data indicate an important role of the inﬂammatory response in the pathogenesis of S. suis infection in mice. This experimental model may be useful for studying the mechanisms
Perception, feedback regarding post-surgical complications of tracheostomy

Introduction: The standard treatment for managing long-term ventilator-dependent patients is still tracheostomy. Lower airway resistance, smaller dead space, less tube movement inside the trachea, greater patient comfort, and more effective suction are all benefits over endotracheal intubation. Methods: In this cross sectional study, data was collected from the purposely constructed questionnaire. The questionnaire was composed of demographical data and questions related to the tracheostomy; ethical approval was obtained, and consent from the respondents was taken, The questionnaire was anonymous and was constructed by the panel of experts including subject specialist, researchers and language specialist. Results: Out of a total 70 patients who underwent tracheostomy, 35% were male while 65% were females, mean (SD) of age was 48.5 (10.2). that have no have mild pain, moderate and have severe controlled Conclusion: We conclude that tracheotomy may be successfully conducted in this group of patients and that it provides significant practical and psychologi cal benefits over other options.
Implementing Project Based Learning Pedagogy in Concrete Industry Project Management

ABSTRACT The objective of this study was to carry out a mixed methods construction education study seeking to improve student learning. This study uses project-based learning (PrBL) and milestone deliverable pedagogy to teach a concrete industry project management course and compares the outcome to a control (non-PrBL) version of the same course. The intervention course was designed as a full semester learning experience for students. The students worked as project managers from start to finish constantly contributing to the completion of their team project. In the control scenario, delivered in Fall 2013, students were required to carry-out a traditional project due at the end of the semester. This study examines the impact of having the course project become the main learning mechanism and considers and measures the ways in which PrBL affects student performance. Pre and post questionnaires regarding self-perceptions of learning and skill development were administered and project and course grades were used to assess the impact of PrBL on the students’ performance. The questionnaires revealed a minimum increase of students’ perception of their understanding of 48%. The course grade comparison showed an average of 13% point increase in all student grades when the PrBL pedagogy was implemented.
Expression of Cyclooxygenase-2 (COX-2) in Urinary Bladder in Rats with Cyclophosphamide (CYP)-Induced Cystitis

These studies examined the expression of cyclooxygenase-2 (COX-2) expression in the urothelium and suburothelial space and detrusor from rats treated with cyclophosphamide (CYP) to induce acute (4 hr), intermediate (48 hr) or chronic (10 day) cystitis. Western blotting and immunohistochemistry were used to demonstrate COX-2 expression. In whole mount preparations of urinary bladder, nerve fibers in the suburothelial plexus and inflammatory cell infiltrates were characterized for COX-2 expression after CYP-induced cystitis. COX-2 expression significantly (p (cid:1) 0.01) increased in the urothelium + suburothelium and detrusor smooth muscle with acute, intermediate and chronic (10 day) CYP-induced cystitis but expression in urothelium + suburothelium was significantly greater. CYP-induced upregulation of COX-2 showed by immunostaining in the urothelium + suburothelium was similar to that observed with western blotting and also demonstrated COX-2 inflammatory cell infiltrates (CD86+) and nerve fibers (PGP+) in the suburothelial plexus. Although COX-2 expression was significantly (p (cid:1) 0.01) increased in detrusor smooth muscle, immunohistochemistry failed to demonstrate an obvious change in COX-2-IR in detrusor muscle but COX-2 inflammatory infiltrates were present throughout the detrusor. COX-2-IR nerve fibers exhibited increased density in the suburothelial plexus with acute or chronic CYP-induced cystitis. COX-2-IR macrophages (CD86+) were present throughout the urinary bladder with acute and chronic CYP-induced cystitis. These studies demonstrate cellular targets in the urinary bladder where COX-2 inhibitors may act. COX-2 complete to the smooth respect to COX-2 upregulation induced the cellular in the urinary that express COX-2 with bladder inflammation of duration. to determine: (1) COX-2 protein expression in the urothelium + suburothelium compared to detrusor smooth muscle by western blotting after CYP-induced cystitis of varying duration; (2) cellular location of COX-2 in urinary bladder of control rats or after CYP-induced cystitis using immunohistochemistry with an emphasis on urothelial cell, nerve fiber and inflammatory cell infiltrate expression; (3) intensity of COX-2 immunoreactivity in the urothelium after CYP-induced cystitis using semi-quantitative image analysis. In contrast to COX-2 expression in detrusor smooth muscle cells with outlet obstruction the present study demonstrates robust expression of COX-2 protein in the urothelium, inflammatory cell infiltrates and to a lesser extent, detrusor smooth muscle with CYP-induced cystitis.
Irrigation and Productivity: Empirical Insight of Farming Households in Tchien District

The study analyses the efficacy of rainfed and irrigated smallholder farms in Tchien district of Liberia. A total of 120 respondents were randomly selected from 5 rural communities around the major rice-producing areas of Tchien District: Gaye town, New Zleh, Old Zleh, Turglor, and Ziah. A total of 58 irrigation farmers and 58 rainfed farmers, comprising of 61 male and 57 female rice farmers were interviewed. Respondents were randomly drawned from a pool of existing Fadama data in Liberia. The study is empirical, taken under different variables to decide the appropriate agricultural system, and the variables to be employed in determining a successful production system. The study applies both the stochastic frontier analysis (SFA) to determine the efficient utilization of production inputs, and the logit model was used to measure the adoption of an irrigation system. Findings suggest that there is a serious disparity in efficiency between rainfed and irrigated farms, implying that irrigated farms are twice as efficient as rainfed farmers; most of the farmers are likely to adopt irrigation systems. Concerned institutions should work to improve access to capital, encourage irrigation farming, and work to effectively integrate experience farmers with young farmers.
CMAR_A_250166 5313..5322

*These authors contributed equally to this work Objective: To evaluate the expression and function of long noncoding RNA (lncRNA) Prader-Willi region non-protein coding RNA 1 (PWRN1) in human glioblastoma (GBM). Materials and Methods: QRT-PCR was applied to assess PWRN1 expression in human GBM tumors and GBM cell lines. PWRN1 was overexpressed by lentiviral infection in LN-229 and U-251 cells to evaluate its effect on GBM cell proliferation and migration in vitro, and xenograft in vivo. The endogenously competing target of PWRN1, human microRNA-21-5p (hsa-miR-21-5p) was evaluated by dual-luciferase activity assay and qRT-PCR. Also, hsa-miR-21-5p was upregulated in PWRN1-overexpressed GBM cells to evaluate the functional involvement of hsa-miR-21-5p in PWRN1-mediated GBM cell proliferation and migration. Results: PWRN1 was downregulated in both human GBM tumors and GBM cell lines. In LN-229 and U-251, PWRN1 overexpression suppressed cancer cell proliferation and migration in vitro, and xenograft growth in vivo. Hsa-miR-21-5p was demonstrated to be the downstream competing target of PWRN1 in GBM. Conversely, upregulating hsa-miR-21-5p in LN-229 and U-251 cells reversed the tumor-suppressing effects of PWRN1 overexpression. Conclusion: PWRN1 is markedly downregulated in GBM. Overexpressing PWRN1 has tumor inhibitory effect on GBM cells, likely via reversely suppressing the expression of tumor oncogenic factor of hsa-miR-21-5p.
Estimation in Generalized Mixed Models

Regression models containing fixed and random effects may have a response variable which is not normally distributed. The generalized mixed model includes both discrete and continuous response variables and is developed here for problems in which the regression variables enter linearly into the model. Best linear unbiased predictor methods are extended to maximum likelihood and residual maximum likelihood estimation procedures. Applications in modelling discrete response variables and in survival analysis are discussed
Role Ambiguity, Role Conflict, Auditor Competence on Audit Quality: The Mediating Effects of Auditing Planning and Independence

The purpose of this study was to determine and analyze the effect of role ambiguity, role conflict and auditor competence on auditor independence and internal audit quality as well as the effect of auditor independence and audit planning on internal audit quality. It is also to empirically analyze the mediating effects of auditor independence and audit planning in the relationship between independent variables to dependent variable of internal audit quality. The population of this research is auditor inspectorate in Central Sulawesi Province. Sampling used purposive sampling technique with a total of 202 samples. The data were analyzed using the Structural Equation Model (SEM) technique. The results showed that role ambiguity and role conflict had a significant negative effect on auditor independence, but did not significantly affect the quality of internal audit. Auditor competence has a positive effect on auditor independence and internal audit quality. The findings also pointed out that auditor independence and audit planning have a significant positive effect on internal audit quality. Statistical testing of mediating variables showed that auditor independence mediates the relationship between role ambiguity and role conflict and the quality of internal audit. Likewise, mediating variables of auditor independence and audit planning have empirically proven to strengthen the effect of auditor competence on the quality of internal audit.
Developing Technological Pedagogical Content Knowledge (TPACK) in Animal Physiology

The purpose of this study is to describe preservice teacher’s learning during lecturing Animal Physiology and investigate it’s impact on preservice teacher’s technological pedagogical content knowledge (TPACK). How was the lecturing process can improve TPACK of preservice teacher on Biology education espescially in Animal Physiology. There are four experiment classes using Solomon four group design, there are pedagogic treatment, content treatment and technological treatment, the last class without any treatment. Both quantitative and qualitative data were collected. Quantitative data were collected through a questionaire of TPACK. Qualitative data were collected through a lesson plan and teaching simulation. Findings has revealed that participants experienced significant gains in all TPACK constructs. Both of pedagogic and technology treatment is better than others, but pedagogical treatment didn’t also increase PCK most of participants. Findings has implications for teacher education programs to be a professional teachers and for researchers interested.
Interest of beta 2-microglubulin determination in chronic hemodialysis patients

The amylase beta 2-microglobulin (β2m) is a common complication of long-term hemodialysis, mainly responsible for osteo-articular manifestations including carpal tunnel syndrome (CTS). With the aim to study the various parameters involved in the increase of and determine the interest of dosage, we conducted a cross-sectional study of 104 chronic hemodialysis patients for more than 6 months. The mean age of patients was 52.82 years with a male predominance (62.5%). 61, 3% of our patients are anuric. They all have intermittent hemodialysis at 12 hours per week using a low permeability membrane in 52% of patients. The average age of dialysis layout is 42, 18 years. The mean duration of hemodialysis was 74 months. The mean serum β2m is 35, 24 mg / l. regarding complications, there were four confirmed cases of carpal tunnel syndrome and 12 deaths. Our study objectify the parameters involved in the increase of β2m: The current age, age advanced dialysis layout, lack of urine output, low permeability membrane and extended duration hemodialysis. Increased serum levels of β2m in our hemodialysis demonstrate the poor treatment of middle molecules. Its reduction can be made by improving the quality of dialysis and will avoid all the complications.
BACTERIAL AND PLANKTON COMMUNITIES IN MARICULTURE WATER SOURCES: A CASE STUDY IN NAMPU AND SEMBUKAN SEAWATERS, WONOGIRI, INDONESIA

Bacterial and plankton communities in mariculture sources: a case study Vol. Seawaters in Indonesia, part of the tropical marine ecosystem, have great microbial and plankton diversity. Seawater is used as a water source for marine aquacultures, such as shrimp, milkfish, lobster, and mud crab. Sustainability of environmental resources for supporting aquaculture activities can be assessed by analyzing the actual conditions of the water source environment, including bacterial and plankton communities. However, the characteristics of bacteria and plankton communities in Indonesian seawaters have not been well documented. In this study, we investigated the bacterial and plankton communities in surface seawater from two coastal areas, i.e. Nampu and Sembukan, Wono- giri regency, Central Java, Indonesia. Bacterial diversity was analyzed using the Illumina-based high throughput sequencing with a primer set targeting the V3-V4 region of the bacterial 16S rRNA gene. Meanwhile, the plankton community (phytoplankton and zooplankton) was calculated and identified using a counting chamber method. Sequencing analysis revealed that the five dominant bacterial phyla in the two seawater samples were similar, consisting of Proteobacteria, Firmicutes, Actinobacteria, Bacteriodetes, and Cyanobacteria. Although the two sites are separated by several kilometres, the distribution of dominant bacterial phyla in both seawater samples is similar. Phytoplankton in Nampu and Sembukan were similarly dominated by Trichodesmium sp. , Navicula sp. , and Rhabdonema sp. Dominant zooplankton in the two sites were Euterpina , Nauplius , Oithona sp. , Oncaea sp. , Tigriopus sp. , and Gastropoda larvae. The seawater in Nampu and Sembukan is suitable as a water source for aquaculture.
Evaluation of Immunological Reactivity in Patients with Cataract Who Received Low Doses of Ionizing Radiation

Mathematical calculation of the six integral hematological indices of the leukogram showed the decrease of functional metabolic and phagocytic activity of micro and macrophages in cataracts of Liquidators of the Accident Consequences (LAC) on Chernobyl Nuclear Power Plant (CNPP), who live in Kazakhstan (Astana city) and in workers of the atomic industry of the Stepnogorsk Mining And Chemical Combine (SMCC), RK on the extraction and processing of concentrates of enriched uranium. The specificity of the process of autosensibilization leukocyte antigens of the lens, among these people was estimated in the reaction of the leukergy stimulated by lens antigens in vitro . Leukergy significantly manifested in LPA exposed to ionizing radiation at a dose of 3.3 to 18.2 cGy, and ranged from 5.7% to 8.0% and 9.0% of the workers «SMCC» at a total effective dose of 61,16±21,64 mSv, with 1.0% in the control group. Thus, the proposed method of leukergy in the presence of lens antigens in vitro is quite informative and resource useful for the empowerment of the differential diagnosis of cataract of radiation genesis.
Austin Journal of Cardiovascular Disease and Atherosclerosis Atrial Fibrillation in India: Is it a Tide Rising or a Tsunami?

Atrial Fibrillation (AF) is the most common sustained cardiac arrhythmia in the general population [1–3]. It affects 1-1.5% of the population in the developed world. The prevalence of AF is strongly dependent on age. Framingham Heart Study investigators estimated that the overall lifetime risk for the development of atrial fibrillation was one in four after the age of 40 years and was one in six in the same age ,even in the absence of preceding heart failure or myocardial infarction [4]. Systematic global surveillance of AF is necessary for better diagnosis and management of the condition.
Construction and Validation of Nomogram for Prediction of Metastasis in Osteosarcoma Patients

Background: Osteosarcoma is one of the most common malignant tumors of the bone with poor prognosis. The present study was aimed to establish and validate nomogram to predict the risk of metastasis for individual osteosarcoma patients.Methods: 2195 osteosarcoma patients were retrospectively collected from Surveillance, Epidemiology, and End Results (SEER) database. The least absolute shrinkage and selection operator regression model was applied to promote feature selection for the metastasis signature model. Univariate and multivariate logistic analysis were used to discern independent predictive factors. These predictive factors were included in the nomogram to evaluate metastasis probability in osteosarcoma patients. Nomogram was validated internally and externally. Results: We randomly assigned 2195 osteosarcoma patients into the training (n = 1464) and validation (n = 731) subgroups. Age, sex, tumor grade, tumor size and use of surgery were identified as the predictive factors for the risk of metastasis (all p<0.05) and further incorporated to develop the nomogram. Predictive accuracy (AUC) for metastasis prediction were 0.7427067 in the training subgroup and 0.6798374 in the external validation subgroup, respectively. And the decision curve analysis also proved the value of the model.Conclusions: We have constructed new high-performance nomogram to assist clinicians in identifying osteosarcoma patients with high risk of metastasis.
Effector Function Precursor Frequency and Subsequent T Cell + Influenza Epitope-Specific CD8 Effect of MHC Class I Diversification on

Earlier studies of inﬂuenza-speciﬁc CD8 + T cell immunodominance hierarchies indicated that expression of the H2K k MHC class I allele greatly diminishes responses to the H2D b -restriced D b PA 224 epitope (acid polymerase, residues 224–233 complexed with H2D b ). The results suggested that the presence of H2K k during thymic differentiation led to the deletion of a prominent V b 7 + subset of D b PA 224 -speciﬁc TCRs. The more recent deﬁnition of D b PA 224 -speciﬁc TCR CDR3 b repertoires in H2 b mice provides a new baseline for looking again at this possible H2K k effect on D b PA 224 -speciﬁc TCR selection. We found that immune responses to several H2D b - and H2K b -restricted inﬂuenza epitopes were indeed diminished in H2 bxk F 1 versus homozygous mice. In the case of D b PA 224 , lower numbers of naive precursors were part of the explanation, though a similar decrease in those speciﬁc for the D b NP 366 epitope did not affect response magnitude. Changes in precursor frequency were not associated with any major loss of TCR diversity and could not fully account for the diminished D b PA 224 -speciﬁc response. Further functional and phenotypic characterization of inﬂuenza-speciﬁc CD8 + T cells suggested that the expansion and differentiation of the D b PA 224 -speciﬁc set is impaired in the H2 bxk F 1 environment. Thus, the D b PA 224 response in H2 bxk F 1 mice is modulated by factors that affect the generation of naive epitope-speciﬁc precursors and the expansion and differentiation of these T cells during infection, , NS2 tetramers (ImmunoID) allophycocyanin for 1 h After cells were stained for CD8 a and, in some instances, for CD62L. For analysis of V b usage, splenocytes from naive mice or tetramer-stained splenocytes from mice sampled on day 10 after HKx31 infection were stained with anti–CD8 a -PerCPCy5.5 (53-6.7; BD Pharmingen) and a panel of 14 FITC-conjugated mAbs speciﬁc for various V b families (BD Pharmingen). To identify Ag-experienced CD8 + T cells, we stained splenocytes with anti–CD8 a -allophycocyanin (53-6.7; Bio- Legend) and anti–CD11a-FITC (2D7; BioLegend). For analysis of cell surface H2D b and H2K k expression, splenocytes from naive mice were treated to lyse erythrocytes and then incubated in Fc block (spent 2.4G2 supernatant with 0.5% normal mouse serum and 0.5% normal rat serum). Cells were then stained with anti–H2D b -FITC (KH95; BD Pharmingen) or anti–H2K k -FITC (36-7-5; BioLegend). Flow cytometric analysis was performed on a BD FACSCalibur (BD Biosciences), and the data were analyzed by either CellQuest Pro (BD Immunocytometry Systems) or FlowJo (Tree Star) software.
Socio-Demographic Factors Responsible for Poor Management of Diabetes Mellitus

Objective: To determine the socio-demographic factors, responsible for poor treatment of diabetes mellitus in patients with uncontrolled diabetes Methodology: This cross-sectional study was conducted at BHU Inayat Pur, with six months duration from November 2016 to April 2017. All the patients with uncontrolled diabetes mellitus, poor treatment history either gender were included in the study. All the selected patients were interviewed in the OPD regarding socio-demographic characteristics. Patients were also interviewed regarding reasons of poor treatment. All the responsible socio-demographic factors were recorded in the proforma. Results: Total 65 patients were selected and mean age of the patients was 47.34+6.11 years. Male were found in the majority 40(61.5%). Mostly patients 42(64.6%) were presented with poor socioeconomic condition. The poor socioeconomic status was the main responsible factor found in 17(26.2%) patient, followed by no available family physicians 03(04.6%), can ’t travel to health facilities 05(07.7%), no any care taker 03(04.6%), under Homeo treatment 06(09.2%), 06(09.2%) don’t continue treatment due to ignorance and eat everything in sweet. 14(36.9%) cases said that they can’t take drugs for long time, 07(10.7% ) were not under proper treatment due to relative advises, while 04(06.1%) said doctor advised them insulin but they can’t afford. Conclusion: It is concluded that poor socio-economic status, drugs use for long time and ignorance are frequent responsible factors for poor treatment of diabetes mellitus.