Miracle-dz/UniFAR-FTUs huggingface dataset:
Allozymic and Isozymic Evidence for Polytypy in the North American Catostomid Genus Cycleptus

Abstract To assess patterns of geographic variation in the wide-ranging North American catostomid genus Cycleptus, 23 specimens were obtained from six locations among four drainage systems that exit into the Gulf of Mexico. Starch gel electrophoresis was used to compare gene products of 47 loci. A contingency chi-square test showed that the 23 samples did not come from a single, random-breeding population. Tests using exact probabilities showed that all samples from within each of the four drainage systems were in agreement with Hardy-Weinberg equilibrium expectations. Pairwise combinations showed that only two of the drainages could be combined and still result in equilibrium. Three allopatric genetic units are recognized: (1) Rio Grande drainage, (2) Mississippi drainage, and (3) the Alabama plus Pascagoula drainages. All three units can be distinguished by differences in allelic composition at certain enzymatic loci, levels of heterozygosity, number of genes expressed (some genes have been silenced in these allotetraploids), and perhaps a regulatory character involving mMdhp-A1. We propose that these units be recognized as separate species. The Mississippi drainage population would retain the name Cycleptus elongatus (Lesueur). The Gulf Coast population has recently been described as Cycleptus meridionalis Burr and Mayden. The Rio Grande population awaits further analysis of morphological and molecular variation and a formal description.
Expression of novel ascorbate peroxidase isoenzymes of wheat ( Triticum aestivum L.) in response to heat stress

Active oxygen species are produced by some of the important organelles like chloroplast and mitochondria through their electron transport chain even under normal environmental condition but the rate of active oxygen species (AOS) production increases many times in response to different abiotic stresses. Plants has antioxidant enzymes system like ascorbate peroxidase (APX), catalase, SOD, GR etc. which helps in reducing the AOS produced inside the plant system in order to protect the key enzymes from AOS. In present investigation, expressions of many new proteins were observed in thermo tolerant (8 in case of C306) and susceptible cultivars (2 in case of PBW343) at different stages in response to heat stress. Isoenzymic profile of APX in response to differential heat shock revealed the expression of 8 APX isoenzymes in C306 (thermo tolerant) compare to 2 in PBW343 (susceptible) cultivars of wheat. A stage specific study revealed the expression of 5 APX isoenzymes at vegetative, pollination, milky dough and seed hardening stages (C306) whereas, 3 APX isoenzymes were observed during milky dough and seed hardening stages in PBW343. It is clear now that high expression of endogenous APX isoenzymes supplements the antioxidant enzyme systems of the plant and protects the plant from oxidative damage in response to biotic and abiotic stresses.
Effect of Conflict, Job Satisfaction, and Stress on Medical Staff Performance (Study in the Health Facilities, West Jakarta)

This study aims to determine the effetc of work conflict, job satisfaction and work stress on the performance of medical personnel. The population in this study were medical personnel oncluding doctors, nurses, midwives, nutritionists, labolatory analysts, radiographers, and pharmacists. This research was limited to respondents of medical personnel who worked in Health Care Facilities in the West Jakarta Region. The Sampling method uses purposive sampling with a nonrandom sampling technique, as many as 205 respondents. The method of data collection is done by distibuting quesionnaries online. Data processing uses software Lisrel 22 for structural equation model (SEM). The results of the research hypothesis show there are significant effects of conflict, job satisfaction and work stress on the performance of medical personnel. Of the three variables, which most influence the performance of medical personnel is the variable job satisfaction.
DNA microsatellite analysis of genetic diversity among Chinese indigenous laying-type ducks (Anas platyrhynchos)

The genetic polymorphisms of 17 microsatellites were investigated in four indigenous laying-type duck breeds in China. The average number of alleles ( Na) and average rates of homozygotes of each breed were counted. Accordingly, allele frequencies of the 17 microsatellites, polymorphism information content ( PIC), mean heterozygosity (H) and genetic distances () were also calculated. Moreover, dendrograms using UPGMA and the neighbour-joining method were produced. The four breeds have a high average PIC (0.643) and H (0.682). Ds are between 0.514 and 0.662, the gene differentiation among the four breeds is 14.4%.
Expression of αIIbβ3 Integrin (GPIIb-IIIa) in Myeloid Cell Lines and Normal CD34+/CD33+ Bone Marrow Cells

Regulation of myeloid cell proliferation and differentiation in the bone marrow is mediated, in part, by the interaction of integrins on early myeloid cells with the extracellular matrix proteins secreted by stromal cells. To further define adhesive protein receptors of early myeloid cells, we examined the expression of the integrin GPIIb-IIIa (alphaIIbbeta3) in leukemic cell lines KG-1a, KG-1, and HL-60, that represent early stages of myeloid differentiation. All three cell lines expressed surface GPIIb-IIIa as measured by flow cytometry and by binding of 125I-anti-GPIIb-IIIa monoclonal antibody. Preincubation of cells with human AB serum or platelet releasate increased GPIIb-IIIa surface expression. GPIIb transcripts were identified in all three cell lines by Northern blot analysis. Furthermore, we readily detected GPIIb transcripts in fluorescence activated cell sorted (FACS) myeloid cells from normal human bone marrow by RT-PCR. Cloning and sequencing of the PCR products established the identity of GPIIb transcripts in the leukemic cell lines and CD34+/CD33+ normal bone marrow cells. Since the normal myeloid cells also demonstrated markers corresponding to the maturational stage of KG-1a/KG-1 cells, we propose that GPIIb-IIIa may serve as a myeloid differentiation antigen and as a key integrin of myeloid precursors.
Species-specific phytoplankton responses to nutrients and zooplankton manipulations in enclosure experiments

SUMMARY 1. In situ enclosure experiments were performed in the mesotrophic Bermejales reservoir to evaluate the algal response to changes in the nutrient supply and in the zooplankton size structure and density in a 2 × 2 factorial design. The experiments were conducted during the spring bloom of nanoplanktonic diatoms in 1989. 2. Nutrient enrichment promoted a great increase of phytoplankton biomass indicating a strong nutrient limitation on phytoplankton growth. Total phytoplankton biomass was significantly lower in the Daphina-added enclosures at a given nutrient level and strong direct an indirect effect of zooplankton on phytoplankton community structure and nutrient availability were observed. 3. Most of the nanoplanktonic species were effectively grazed but species with protective coverings and large size colonies were favoured by grazers and small chlorococcales were unaffected probably because of their compensatory high growth rates. The decrease in total biomass imposed by grazers is attributable mainly to the decrease of Cyclotella ocellata, the most abundant species. This taxon suffers two net effects of zooplankton: direct grazing and the indirect decrease of Si availability caused by the growth of C. ocellata which was promoted by P excretion by zooplankton. Indirect effects of grazers on Si availability should, therefore, be taken into account in explaining phytoplankton succession and community structure. 4. In this experiment grazers affected considerably the nanoplanktonic community in Bermejales reservoir. The extent which they were affected, however, depended not only on the algal size as a determinant of edibility but also greatly on the specific nutrient requirements and taxonomic features of the algal species.
Taxation System, Tax Sanctions, Justice, Discrimination, and Probability of Cheat Detect Impact on Taxpayer Perception Regarding Tax Evasion Ethics (Study at Primary KPP in Yogyakarta)

This study is about Yogyakarta. Sleman as One of the regency in Yogyakarta had not enough regional original income (PAD) in order to invest in capital expenditure, especially for the information technology. So that collecting and maximizing the potential original incomes (PAD), especially regional taxes then is less than optimal. It is important to examine how the attitude or ethics of taxpayers in Yogyakarta to ensure information technology is urgently used as a driving force for increasing PAD, especially regional taxes. Using questionnaires distributed to the taxpayers of Pratama tax office (KPP) in Yogyakarta, we collect data to obtain primary data representing perceptions of taxpayer attitudes and ethics in Yogyakarta. Using the data from the questionnaires we examined and analyzed whether empirical taxation systems, taxation sanctions, fairness, discrimination, and probability of cheat detect is related to the taxpayer's perception of the ethics of tax evasion. The research population is all individual taxpayers registered with the KPP Pratama in Yogyakarta. We used the convenience sampling method. The data analyzed by multiple linear regression. The result is: (1). the taxation system has a negative influence on the perception of taxpayers regarding the ethics of tax evasion; (2). tax sanctions do not have a significant positive influence on the perception of taxpayers regarding the ethics of tax evasion; (3). justice has a positive influence on the perception of taxpayers regarding the ethics of tax evasion; (4). discrimination does not have a negative influence on the perception of taxpayers regarding the ethics of tax evasion; (5). probability of cheat detect has a negative influence on the perception of taxpayers regarding the ethics of tax evasion. Interesting findings that the probability of cheat detect causes taxpayers to ethically embezzle taxes. Our hope is that information technology drives PAD, especially regional taxes, because it reduces cheating probability.
Efficient Procedure for In vitro Microrhizome Induction in Curcuma longa L. (Zingiberaceae) - A Medicinal Plant of Tropical Asia

An improved in vitro microrhizome induction system in Curcuma longa L. has been developed. Freshly sprouted axillary buds from underground rhizomes were used as initial explants and multiplied through established in vitro systems. Multiplied shoots were excised and subcultured on hormone free medium for four weeks to induce microrhizome. Effects of light, sucrose and growth regulators on in vitro microrhizome production have been studied. Nine per cent sucrose was found to be the most suitable for microrhizome production, when incubated in the dark. Various concentrations of BA and Kn, and NAA were tested. BA (12.0 µM) and NAA (0.3 µM) were found suitable for the induction of microrhizomes. Larger microrhizomes performed better under in vivo conditions and developed shoots readily, when they were transferred directly from in vitro to soil without acclimatization. Microrhizomes produced under in vitro conditions can be stored and transported easily which are advantageous for in vitro shoot multiplication.
Síntesis del conocimiento taxonómico, origen y domesticación del género Capsicum

The genus Capsicum consists of approximately 30 species of which C. annuum, C. chinense, C. frutescens, C. baccatum and C. pubescens are domesticated. Although Capsicum has been studied from the taxonomic and evolutionary point of view, using morphological, cytological and molecular characters, there are still sorne problems related to the taxonomic delimitation of the genus and its species, the nomenclature of the wild and cultivated forms, and the treatment of infraspecific variation. In order to determine the generic and specific taxonomic limits and to establish their phylogenetic and evolutionary relationships, it is necessary to undertake these studies among all the taxa of the genus Capsicum. C. annuum, C. chinense, and C. frutescens are taxa that form a taxonomic complex that can be barely differentiated and at the present it is impossible to determine whether they belong to the same or different species. The center of origin of the genus Capsicum is in South America, in the region that comprises Bolivia, northern Argentina, and central and southern Brazil. The biogeographic and archeobotanical studies indicate that during the dispersion of Capsicum along the American Continent, some of the species were domesticated independently in different places: C. annuum in Mexico; C. frutescens in Costa Rica, and possibly also in Mexico; C: chinense in the Amazonas lowlands; C. baccaturn in Bolivia, and C. pubescens in the Andes. In Mexico, C. annuwn has been cultivated throughout all the country; C. frutescens in the central and south eastern regions; C. chinense in the Yucatan Peninsula, and C. pubescens in the high lands of the central states. In addition, there are C. ciliatum and C. lanceolatum, two species that have never been used by man. Genetic and ecological studies on wild populations are very important for the use and conservation of this genetic resource.
Towards Generalization in Target-Driven Visual Navigation by Using Deep Reinforcement Learning

Among the main challenges in robotics, target-driven visual navigation has gained increasing interest in recent years. In this task, an agent has to navigate in an environment to reach a user specified target, only through vision. Recent fruitful approaches rely on deep reinforcement learning, which has proven to be an effective framework to learn navigation policies. However, current state-of-the-art methods require to retrain, or at least fine-tune, the model for every new environment and object. In real scenarios, this operation can be extremely challenging or even dangerous. For these reasons, we address generalization in target-driven visual navigation by proposing a novel architecture composed of two networks, both exclusively trained in simulation. The first one has the objective of exploring the environment, while the other one of locating the target. They are specifically designed to work together, while separately trained to help generalization. In this article, we test our agent in both simulated and real scenarios, and validate its generalization capabilities through extensive experiments with previously unseen goals and unknown mazes, even much larger than the ones used for training.
Post-nesting movement of wild and head-started Kemps ridley sea turtles Lepidochelys kempii in the Gulf of Mexico

Nesting by the endangered Kemp's ridley sea turtle Lepidochelys kempii has increased on the Texas coast during recent years. Movements of 28 individual adult females that nested on North Padre Island or Mustang Island, Texas, USA, were monitored using satellite telemetry. Plat- form transmitter terminals (PTTs; n = 36) were deployed on the 28 turtles between 1997 and 2006, with 1 individual receiving 3 PTTs during different years, 6 receiving 2, and 21 receiving 1. Of the 28 individuals, 17 were from the wild stock, 9 were head-started turtles (reared in captivity for the first months of life) that had been experimentally imprinted to Padre Island as hatchlings, and 2 were head-started turtles that had been obtained directly from Mexico as hatchlings. Locations were obtained from 9 to 841 d (mean = 277 d) following deployment. After they completed nesting for the season, most of the tracked turtles left south Texas and traveled northward, parallel to the coastline, with their last identified location in the northern or eastern Gulf of Mexico. Inter-nesting residency was documented off south Texas and post-nesting residency in USA Gulf of Mexico waters from south Texas to the tip of Florida. Movements and habitat utilization by wild and head-started turtles and by individuals during different tracking events were generally similar. However, all of the 5 tur- tles that briefly traveled southward to waters off the coast of Mexico were wild. Tracking data were used to aid with nest detection and protection, and development of a regulation by Texas Parks and Wildlife Department that closed near-shore south Texas waters to shrimp trawling. Findings from this study demonstrate the importance of near-shore Gulf of Mexico waters, particularly offshore from south Texas, to post-nesting Kemp's ridley turtles.
Talaromyces verruculosus, A Novel Marine Fungi as a Potent Polyhydroxybutyrate Degrader

Fungi were iso lated from Marine water collected from three different regions of Andhra Pradesh, India. A total number of 45 fungi were isolated of which 9 showed varied sized clear zones on assay medium. Largest clear zone was detected below and around the fungal colon y WFPHB2, T.verruculosus for which the PHB (Polyhydroxybutyrate ) depolymerase productivity has not yet been published. This strain grew well on PDA (Potato Dextrose Agar ) medium . I n early stage of growth, enzyme production was highest at 30 o C. Purificati on and characterization of PHB depolymerase secreted by WFPHB2 was carried which reported that single protein band of 45 kDa is responsible for depolymerase activity. 80µl of the purified enzyme showed clear degradation zone during plate assay. 96 hrs incu bation in liquid broth containing WFPHB2 resulted in 90%depolymerization of PHB films.
INDIVIDUAL AND POPULATION-LEVEL VARIABILITY IN DIETS OF PALLID BATS (ANTROZOUS PALLIDUS)

Abstract At 2 locations in California (coastal, Tocaloma; desert, Caliente), analysis of feces presented a significantly higher number of prey types for the diets of Antrozous pallidus than analysis of culled parts of prey. Analysis of diet by culled parts was biased toward larger, harder prey, and some softer, smaller prey were missed altogether. Observation of individuals feeding revealed that some bats ate prey without culling any parts, whereas others culled only the hardest and largest parts. Analysis of feces from tagged adult male pallid bats from Tocaloma (1993–1994) and Caliente (1994–1995) suggested that bats were generalists, but whereas diets of individuals at Caliente reflected the average diet for the group, none of the individuals at Tocaloma ate the average diet. Variation in the diets of A. pallidus reflects prey availability and individual foraging behavior. Tocaloma bats did not significantly change their diets throughout summer; Caliente bats did. Bats from Caliente and Tocaloma ate different prey than arthropods caught in pit traps, suggesting that bats in both populations were selective foragers. In captivity, hunting A. pallidus took flying and nonflying prey. Some flying prey were forced against a surface before capture, adding a novel dimension to the range of behavior involved in “gleaning. ”
Serum Biochemical Parameters of Female Bronze Turkeys (Meleagris gallopavo) During Egg-Laying Season

6 Abstract: Serum biochemical values of uric acid, urea, total proteins, albumin, globulin, Aspartate Aminotransferase (AST) and Gama Glutamiltransferase (GGT) were estimated in blood samples collected from 15 female Bronze Turkeys during the laying season (32-52 weeks of age). Significant differences were observed for total proteins, globulins, AST, urea and uric acid during the laying period evaluated. The major differences noted were due to elevations on total protein, globulins and uric acid concentrations in 52 week- old females.
Transformation of a high-molecular-weight (HMW) glutenin subunit Dy10 gene into maize

Particle bombardment has been used to transform maize genotype Hi-II. Immature embryos were co-transformed with a plasmid containing the selectable and scorable marker genes (bar and uidA, respectively) and a plasmid containing the high-molecular-weight glutenin subunit Dy10 gene. Eight transgenic events (T0) were recovered from 1000 bombarded scutella (transformation efficiency thus 0.8%). T1 generation was produced by cross pollination between T0 plants and nontransgenic plants of the Egyptian inbred line Sd63. Integration of trangenes has been confirmed in the genome of T0 plants by PCR and Dot blot hybridization analysis. Expression of marker genes was detected in T0 plants by leaf painting and histochemical staining for bar and uidA genes, respectively.
Anthropometric Study of Human Hip Bones of Southern Brazilians by Rabbi Method

Forensic anthropology is a branch of forensic sciences that deals with research and aims to determine the identity of an individual by means of the origin of remains, general patterns of identification and individualizing characteristics. Currently, as Brazil has a significant percentage of homicides, serious accidents and natural disasters, it is necessary to use more precise anthropometric techniques to search for victim identification. Therefore, this study proposes to measure human hip bones using bone anthropometry technique, more specifically pelvimetry in order to determine standard measures for this bone for people of the south region of Minas Gerais. The measures obtained were as follows: VDA data was 49.74 ± 3.49 for right hip bone (RHB) and 51.06 for left hip bone (LHB). GSND data was 33.18 ± 3.08 for RHB and 32.96 ± 3.35 for LHB. Regarding CGSN measure, the values were 81.23 ± 6.74 for RHB and 82.87 ± 6.77 for LHB. DASIS results were 114.1 ± 6.62 for RHB and 112.4 ± 5.90 for LHB. OFD measures were 45.95 ± 4.21 for RHB and 47 ± 3.98 for LHB while OFL data were 31.34 ± 3.56 for RHB 30.42 ± 2.82 for LHB. From the results above, one could conclude that there were significant differences in the measure of the hip bone when compared with data Brazilian Journal of Forensic Sciences, Medical Law and Bioethics 9(3):356-365 (2020) 357 M. O. Romão et al. published for other ethnic groups. It is suggested that such differences may be related to the multiple genetic miscegenation that occurred in this region of Brazil during 500 years or more after colonization.
Resistência e suscetibilidade de bovinos leiteiros mestiços ao carrapato boophilus microplus

Counts of the cattle tick Boophilus microplus were made on a crossbred dairy herd raised at the “Estacao Experimental de Zootecnia de Colina”, north of Sao Paulo State, Brazil, in the middle of each season, during two years. The objective of the study was to assess for some genetic and environmental effects that might be affecting tick resistance on the herd. Counts of female ticks more than 4.5 mm in length were analysed by least squares method (n = 4.218), using counts (x) transformed to In 2 (x + 1). The results showed significance (P < 0.01) for the effects of sire, season, sex, age, pasture and grass species. Natural infestation levels increased in summer and decreased in winter, with a peak in fall. Four strategic dippings, realized at 21 days interval just after the spring count, reduced tick infestation in the following season. Males were more susceptible than females. Unweaned calves aged 2-3 months were highly resistant to the tick, while those aged 8 to 12 months and more than 4 years old were more susceptible. Animals grazing on newly-formed or spelling pastures had lower tick infestation than those on continuous grazing pastures. Animals grazing on pastures of “coloniao” grass (Panicum maximum Jacq cv. Coloniao) had the lowest tick counts. Overall means of tick counts and heritability were 10 and 0.089 ±0.030, respectively.