Miracle-dz/UniFAR-FTUs huggingface dataset:
The impact of Alzheimer’s disease risk factors on the pupillary light response

Alzheimer’s disease (AD) is the leading cause of dementia, and its prevalence is increasing and is expected to continue to increase over the next few decades. Because of this, there is an urgent requirement to determine a way to diagnose the disease, and to target interventions to delay and ideally stop the onset of symptoms, specifically those impacting cognition and daily livelihood. The pupillary light response (PLR) is controlled by the sympathetic and parasympathetic branches of the autonomic nervous system, and impairments to the pupillary light response (PLR) have been related to AD. However, most of these studies that assess the PLR occur in patients who have already been diagnosed with AD, rather than those who are at a higher risk for the disease but without a diagnosis. Determining whether the PLR is similarly impaired in subjects before an AD diagnosis is made and before cognitive symptoms of the disease begin, is an important step before using the PLR as a diagnostic tool. Specifically, identifying whether the PLR is impaired in specific at-risk groups, considering both genetic and non-genetic risk factors, is imperative. It is possible that the PLR may be impaired in association with some risk factors but not others, potentially indicating different pathways to neurodegeneration that could be distinguished using PLR. In this work, we review the most common genetic and lifestyle-based risk factors for AD and identify established relationships between these risk factors and the PLR. The evidence here shows that many AD risk factors, including traumatic brain injury, ocular and intracranial hypertension, alcohol consumption, depression, and diabetes, are directly related to changes in the PLR. Other risk factors currently lack sufficient literature to make any conclusions relating directly to the PLR but have shown links to impairments in the parasympathetic nervous system; further research should be conducted in these risk factors and their relation to the PLR.
Dengue Fever Perception among Undergraduate Students at a Public Sector University, Rawalpindi

Background: Dengue is the most widely distributed mosquito-borne viral disease in the world. It continues to spread rapidly because of climate change, rapid urbanization, and population growth. In the world population ranking Pakistan stands at number 6th position, having subtropical position. Due to growing cases, this study is undertaken to evaluate the perception of Dengue fever among the university students. Engagement of students and community to improve participation and mobilization for vector control is a key element to regulate the control interventions and strategies. Methods: A descriptive cross-sectional study was carried out at National University of Medical Sciences Rawalpindi during July-Nov 2022 including 346 participants. Online survey was conducted using close ended structured questionnaire by using nonprobability convenience method. The age, gender, living status, accommodation status, and residential area of the students together with perceptions of undergraduate students regarding Dengue’s knowledge and prevention was recorded. Based on students’ correct answers, overall knowledge level assessment and prevention awareness level was calculated. Association of categorical variables were analysed using chi-square test. Statistical data analysis was carried out using commercially available software. Results: Out of 346 students, 58.6% had knowledge of dengue and 84.9% were aware about its prevention. The multi-variate analyses revealed that positive relationship exists between Dengue’s knowledge with age, gender, and residential area and between Dengue’s prevention awareness with age and gender (p<0.05). Conclusion: The overall knowledge and awareness of students regarding Dengue fever was found to be adequate, being higher among male than female students. The collaborative effort by all stakeholders can contribute towards enhancing the knowledge and awareness among students which will further reduce the risk of disease.
Water contents in pyroxenes of intraplate lithospheric mantle

Water contents of clinopyroxene and orthopyroxene in mantle peridotites from various xenolith occurrences in intraplate settings (both oceanic and continental) were determined by Fourier Transform Infrared Spectroscopy (FTIR). Samples from the following localities were studied: Sal Island (Cape Verde Archipelago); Baker Rocks and Greene Point (Northern Victoria Land, Antarctica); Panshishan and Lianshan (Subei Basin, Eastern China). They represent well-known localities where detailed petrographical and geochemical studies have already been carried out or areas which are currently under investigation. The water incorporated in these pyroxenes is low (cpx, 37–399 ppm; opx, 9–166 ppm) or very low (as in Greene Point, Antarctica; cpx, 5–16 ppm; opx, 9–16 ppm). Within each population there is no clear correlation with melting parameters such as MgO contents in any single mineral. Results are compared with the available literature data on water contents in mantle pyroxenes, which include peridotites from on-craton (hosted by kimberlitic-type magmas) and off-craton (hosted by alkaline basic magmas), as well as subarc mantle settings. The “relatively dry” (cpx, 140–528 ppm; opx, 38–280 ppm) sub-arc mantle xenoliths (Peslier et al. , 2002) are shown to be wetter than the intraplate (off-craton) xenoliths. Cratonic mantle pyroxenes are only represented by a few determinations on garnet peridotites and eclogite from Kaapvaal and Colorado Plateau. They record the highest water contents (cpx, 342–1012 ppm; opx, 180–491 ppm) so far measured in mantle pyroxenes from various tectonic settings. Despite the limited data set, the indication that the cratonic mantle is strongly hydrated is compelling. Rehydration of cratonic mantle may be related to plate subduction ( i.e. , Colorado Plateau) or alternatively to metasomatic enrichment ( i.e. , Kaapvaal Craton). However, assuming that the water content was initially very low, metasomatic events in intraplate settings, similar to those presented here, would not be able to produce a significant increase in pyroxenes water content. According to our own data and those reported in the literature, it appears that substantial rehydration may instead occur at convergent plate margins.
Evaluation of Precision and Sensitivity of Back Extrusion Test for Measuring Textural Qualities of Cooked Germinated Brown Rice in Production Process

The textural qualities of cooked rice may be understood as a dominant property and indicator of eating quality. In this study, we evaluated the precision and sensitivity of a back extrusion (BE) test for the texture of cooked germinated brown rice (GBR) in a production process. BE testing of the textural properties of cooked GBR rice showed a high precision of measurement in hardness, toughness and stickiness tests which indicated by the repeatability and reproductivity test but the sensitivity indicated by coefficient of variation of the texture properties. The findings of our study of the effects on cooked GBR texture of different soaking and incubation durations in the production of Khao Dawk Mali 105 (KDML 105) GBR, as measured by BE testing, confirmed that our original protocol for evaluation of the precision and sensitivity of this texture measurement method. The coefficients of determination (R2) of hardness, toughness and stickiness tests and the incubation time at after 48 hours of soaking were 0.82, 0.81 and 0.64, respectively. The repeatability and reproducibility of reliable measurements, which have a low standard deviation of the greatest difference between replicates, are considered to indicate high precision. A high coefficient of variation where relatively wide variations in the absolute value of the property can be detected indicates high sensitivity when small resolutions can be detected, and vice versa. The sensitivity of the BE tests for stickiness, toughness and hardness all ranked higher, in that order, than the sensitivity of the method for adhesiveness, which ranked lowest. The coefficients of variation of these texture parameters were 31.26, 20.59, 19.41 and 18.72, respectively. However, the correlation coefficients among the texture properties obtained by BE testing were not related to the precision or sensitivity of the test. By obtaining these results, we verified that our original protocol for the determination of the precision and sensitivity of food texture measurements which was successfully used for GBR texture measurement.
Autologous Platelet Lysate Is an Alternative to Fetal Bovine Serum for Canine Adipose-Derived Mesenchymal Stem Cell Culture and Differentiation

Simple Summary Fetal bovine serum is extracted from cow fetuses and is the primary source of nutrition for cell growth in laboratory cell culture media. Exclusively for research purposes, the fetal bovine serum is harmless, but for transplantation research, it can cause severe immune disease in the host. Keeping this serious ethical and scientific issue in mind, we used allogeneic platelet lysate, a nutritional supplement obtained from the same animal that will be transplanted with its stem cells, for stem cell culture. Cell culture media supplemented with different combinations of fetal bovine serum or allogeneic platelet lysate were used in this study. Parameters such as cell doubling time, cell viability, stem cell expression markers, and gene expression of various markers related to the transformation of stem cells into adipocytes and bone cells. Our results showed that with the use of an autologous platelet lysate, the values of all parameters were far better when compared to fetal bovine serum. This means that autologous platelet lysate can be used as a substitute for fetal bovine serum without changing the differentiation potential of cells, thereby addressing the ethical and scientific issues of fetal bovine serum for regenerative medicine. Abstract The use of fetal bovine serum (FBS) in regenerative medicine raises serious ethical and scientific concerns. We have cultured and differentiated the canine mesenchymal stem cells (cMSCs) in five different media combinations of autologous platelet lysate (A-PL) and FBS; consisting of 0% A-PL and 10% FBS (M-1), 2.5% A-PL and 7.5% FBS (M-2), 5% A-PL and 5% FBS (M-3), 7.5% A-PL and 2.5% FBS (M-4), and 10% A-PL and 0% FBS (M-5). The cMSCs were evaluated for their doubling time, differentiation efficiency, and expression of CD73, CD90, CD105, and PDGFRα. The mRNA expression of NT5E, THY1, ENG, PPARγ, FABP4, FAS, SP7, BGLAP, and SPP1 was also assessed. The results indicated non-significant differences in cellular proliferation/viability; positive expression of surface markers, and PDGFRα with substantial adipo/osteogenic differentiation. The expression of adipogenic (PPARγ, FABP4, FAS), and osteogenic (SP7, BGLAP, SPP1) genes were higher (p < 0.05) in the M5 group. In conclusion, A-PL in cMSCs culture did not negatively affect cellular proliferation and viability but also enhanced their genetic potential for multilineage differentiation. Our results indicate that A-PL can be used as an alternative for FBS to develop potent cMSCs under good manufacturing practice protocol for regenerative medicine.
Utility of Ultrasound-Guided Erector Spinae Plane Blocks for Postoperative Pain Management Following Thoracolumbar Spinal Fusion Surgery

Purpose The primary objective of this study is to determine if ultrasound-guided erector spinae plane blocks (ESPB) prior to thoracolumbar spinal fusion reduces opioid consumption in the first 24 hours postoperatively. Secondary objectives include ESPB effects on administration of opioids, utilization of intravenous patient-controlled analgesia (IV-PCA), pain scores, length of stay, and opioid related side effects. Methods A retrospective cohort analysis was performed on consecutive, adult patients undergoing primary thoracolumbar fusion procedures. Demographic and baseline characteristics including diagnoses of chronic pain, anxiety, depression, and preoperative use of opioids were collected. Surgical data included surgical levels, opioid administration, and duration. Postoperative data included pain scores, opioid consumption, IV-PCA duration, opioid-related side effects, ESPB-related complications, and length of stay (LOS). Statistical analysis was performed using chi-squared and t-test analyses, multivariable analysis, and covariate adjustment with propensity score. Results A total of 118 consecutive primary thoracolumbar fusions were identified between October 2019 and December 2021 (70 ESPB, 48 no-block [NB]). There were no significant demographic or surgical differences between groups. Median surgical time (262.50 mins vs 332.50 mins, p = 0.04), median intraoperative opioid consumption (8.11 OME vs 1.73 OME, p = 0.01), and median LOS (152.00 hrs vs 128.50 hrs, p = 0.01) were significantly reduced in the ESPB group. Using multivariable covariate adjustment with propensity score analysis only intraoperative opioid administration was found to be significantly less in the ESPB cohort. Conclusion ESPB for thoracolumbar fusion can be performed safely in index cases. There was a reduction of intraoperative opioid administration in the ESPB group, however the care team was not blinded to the intervention. Extensive thoracolumbar spinal fusion surgery may require a different approach to regional anesthesia to be similarly effective as ESPB in isolated lumbar surgeries.
Modified Storage Atmosphere Prevents the Degradation of Key Grain Quality Traits in Lentil

Lentil seed coat colour influences market value, whilst germination is associated with crop establishment and hydration capacity with optimal processing outcomes. Storing lentil grain assists growers in managing price fluctuations; however, exposure to oxygen at higher temperatures during extended storage degrades seed coat colour, germination, and hydration capacity. Depleting oxygen prevents such degradation in other crops; however, studies in lentil are limited. This study examined the effects of oxygen-depleted modified atmospheres and temperatures on seed coat colour, germination, and hydration capacity in two red lentil cultivars, PBA Hallmark and PBA Jumbo2, stored for 360 days. Small volumes of lentil grain were placed in aluminium laminated bags filled with nitrogen (N2), carbon dioxide (CO2), or air and stored at either 15 or 35 °C. At 35 °C in an air atmosphere, the lentil’s seed coat significantly (p = 0.05) darkened after 30 days of storage, whereas germination and hydration capacities decreased after 60 days regardless of cultivar. In contrast, N2 and CO2 atmospheres maintained initial seed coat colour, germination, and hydration capacities in both cultivars throughout the study period regardless of temperature. Storing lentil grain in an oxygen-depleted modified atmosphere may assist to maximise returns to grower and maintain key quality traits.
Effects of CO2 enrichment, high nitrogen deposition and high precipitation on a model forest ecosystem in Southern China.

Aims Responses of soil respiration to global change play an important role in global carbon cycling, but the effects of increasing atmospheric carbon dioxide concentration ([CO2]), nitrogen (N) deposition and precipitation on soil respiration in subtropical China are unclear. Our objective was to test the effects of increased [CO2], N deposition and precipitation on soil respiration and to determine how they influence soil respiration in subtropical China. Methods A model forest ecosystem was constructed of six tree species native to South China. The species were exposed to four experimental treatments in open-top chambers beginning March 2005. Three chambers were used for elevated [CO2] (CC), two for high N treatment (NN) and the control (CO) and one for elevated precipitation (HR). The CC treatment was achieved by supplying additional CO2 from a tank until the chambers had a concentration of (700 ± 20) μmol CO2·mol. The NN treatment was achieved by spraying seedlings once a week for a total amount of NH4NO3 of 100 kg N·hm·a. The HR treatment was achieved by weekly irrigation with 100 L water. Important findings For two years, soil respiration displayed strong seasonal patterns with higher values observed in the wet season (April to September) and lower values in the dry season (October to March) in the control chambers (CO) and the CC and NN treatments (p 0.05). The CC enrichment affected soil respiration significantly 1024 植 物 生 态 学 报 www. plant-ecology.com 33 卷 (p<0.05), and there were no significant differences in annual CO2 effluxes between CO and the other two treatments. The annual CO2 effluxes reached 4 241.7, 3 400.8, 3 432.0 and 3 308.4 g CO2·m·a in the CC, NN, HR and CO treatments, respectively. Soil respiration showed diverse responses between dry and wet seasons under different treatments. Higher soil respiration in the CC and NN treatments occurred in the wet season (p<0.05). In the dry season, soil respiration increased in the HR treatment (p<0.05) and decreased in the NN treatment (p<0.05). We found significant exponential relationships between soil respiration rates and soil temperature and significant linear relationships between soil respiration rates and soil moisture (below 15%).
Self-regulation learning as active inference: dynamic causal modeling of an fMRI neurofeedback task

Introduction Learning to self-regulate brain activity by neurofeedback has been shown to lead to changes in the brain and behavior, with beneficial clinical and non-clinical outcomes. Neurofeedback uses a brain-computer interface to guide participants to change some feature of their brain activity. However, the neural mechanism of self-regulation learning remains unclear, with only 50% of the participants succeeding in achieving it. To bridge this knowledge gap, our study delves into the neural mechanisms of self-regulation learning via neurofeedback and investigates the brain processes associated with successful brain self-regulation. Methods We study the neural underpinnings of self-regulation learning by employing dynamical causal modeling (DCM) in conjunction with real-time functional MRI data. The study involved a cohort of 18 participants undergoing neurofeedback training targeting the supplementary motor area. A critical focus was the comparison between top-down hierarchical connectivity models proposed by Active Inference and alternative bottom-up connectivity models like reinforcement learning. Results Our analysis revealed a crucial distinction in brain connectivity patterns between successful and non-successful learners. Particularly, successful learners evinced a significantly stronger top-down effective connectivity towards the target area implicated in self-regulation. This heightened top-down network engagement closely resembles the patterns observed in goal-oriented and cognitive control studies, shedding light on the intricate cognitive processes intertwined with self-regulation learning. Discussion The findings from our investigation underscore the significance of cognitive mechanisms in the process of self-regulation learning through neurofeedback. The observed stronger top-down effective connectivity in successful learners indicates the involvement of hierarchical cognitive control, which aligns with the tenets of Active Inference. This study contributes to a deeper understanding of the neural dynamics behind successful self-regulation learning and provides insights into the potential cognitive architecture underpinning this process.
Digitalisation and Subnational Tax Administration in Nigeria

Recently, there has been an expansion in the deployment of digital systems and digital IDs among taxing authorities. However, little is known about the extent to which such technologies are being adopted, or about whether the data from them is being used strategically to improve tax administration. Even less is known about this in the context of subnational tax administration, although this could be very relevant in some contexts, such as Nigeria. This study investigates the extent of the adoption and strategic usage of data from e-tax systems and digital IDs among state internal revenue services (SIRSs) in Nigeria. Data was collected through qualitative interviews conducted within the SIRSs – one from each of the country’s six geopolitical zones, and within the Federal Inland Revenue Service (FIRS). The qualitative data from the interviews was evaluated using thematic analysis. The findings revealed that there is scope for improvement in the adoption and usage of data from e-tax systems and digital IDs among the SIRSs. It was also found that the extent of adoption and strategic data usage from e-tax systems by SIRSs likely improves states’ per capita internally generated revenue (IGR), but similar insights on the impact of digital IDs have not been obtained. Lastly, it was found that there are some lessons SIRSs could learn from FIRS in terms of strategic use of data from e-tax systems and digital IDs. Specifically, SIRSs need to integrate an audit risk engine and machine learning for performing analytics into their e-tax systems, and also automate the estimation of annual credits for withholding tax suffered, tax refunds and penalties, as well as tax audit management including case selection, allocation of auditors and generating audit reports. Some policy recommendations are offered that are consistent with these findings.