Miracle-dz/UniFAR-FTUs huggingface dataset:
A clade C HIV-1 vaccine protects against heterologous SHIV infection by modulating IgG glycosylation and T helper response in macaques

The rising global HIV-1 burden urgently requires vaccines capable of providing heterologous protection. Here, we developed a clade C HIV-1 vaccine consisting of priming with modified vaccinia Ankara (MVA) and boosting with cyclically permuted trimeric gp120 (CycP-gp120) protein, delivered either orally using a needle-free injector or through parenteral injection. We tested protective efficacy of the vaccine against intrarectal challenges with a pathogenic heterologous clade C SHIV infection in rhesus macaques. Both routes of vaccination induced a strong envelope-specific IgG in serum and rectal secretions directed against V1V2 scaffolds from a global panel of viruses with polyfunctional activities. Envelope-specific IgG showed lower fucosylation compared with total IgG at baseline, and most of the vaccine-induced proliferating blood CD4+ T cells did not express CCR5 and α4β7, markers associated with HIV target cells. After SHIV challenge, both routes of vaccination conferred significant and equivalent protection, with 40% of animals remaining uninfected at the end of six weekly repeated challenges with an estimated efficacy of 68% per exposure. Induction of envelope-specific IgG correlated positively with G1FB glycosylation, and G2S2F glycosylation correlated negatively with protection. Vaccine-induced TNF-α+ IFN-γ+ CD8+ T cells and TNF-α+ CD4+ T cells expressing low levels of CCR5 in the rectum at prechallenge were associated with decreased risk of SHIV acquisition. These results demonstrate that the clade C MVA/CycP-gp120 vaccine provides heterologous protection against a tier2 SHIV rectal challenge by inducing a polyfunctional antibody response with distinct Fc glycosylation profile, as well as cytotoxic CD8 T cell response and CCR5-negative T helper response in the rectum. Description The glycosylation profile of IgG and HIV-resistant T helper response induced by vaccination contribute to HIV vaccine protection. A clade C HIV-1 vaccine Clade C HIV-1 infections are responsible for a large proportion of global HIV-1 burden, and a vaccine is urgently required. Sahoo et al. created a clade C HIV-1 vaccination regimen that they tested in rhesus macaques, consisting of priming with modified vaccinia Ankara (MVA) and boosting with cyclically permuted trimeric gp120 (CycP-gp120). To assess delivery methods, vaccines were administered by parenteral injection or oral administration using a needle-free injector. Both routes of MVA/CycP-gp120 administration provided equivalent protection against mucosal SHIV infection. Reduced risk of infection correlated with distinct env-specific IgG Fc glycosylation profiles, as well as with vaccine-induced TNF-α+ IFN-γ+ CD8+ T cells and TNF-α+ CCR5lo CD4+ T cells. These findings demonstrate that the MVA/CycP-gp120 immunization regimen provides significant protection against heterologous SHIV infection.
Curcumin Upregulates miR-148a to Increase the Chemosensitivity of CD44-Positive Prostate Cancer Stem Cells to Paclitaxel Through Targeting the MSK1/IRS1 axis.

BACKGROUND In men, prostate cancer (PC) is the second most common cause of cancer-related death. However, paclitaxel resistance is a major challenge in advanced PC. Curcumin, a natural antioxidant, has been demonstrated to have cytotoxic effects on cancer stem cells (CSCs). The goal of this study is to explore if curcumin can help lower chemoresistance to paclitaxel through the regulation of miR-148a-mediated apoptosis in prostate CSCs. METHODS The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and 4',6-diamidino-2-phenylindole (DAPi) labeling were used to determine cell survival. Immunohistochemistry was used to detect the expression of P-glycoprotein protein (P-gp) and CD44 proteins. Finally, real-time PCR was used to evaluate the regulatory effects of curcumin and paclitaxel on miR-148a and its target genes. RESULTS Curcumin and paclitaxel co-treatment significantly reduced the IC50 value in CD44+cells compared to paclitaxel alone. Additionally, combining these drugs considerably increased apoptosis in CD44+cells. We also discovered that when curcumin and paclitaxel were combined, the expression of CD44 and P-gp was significantly reduced compared to paclitaxel alone. Curcumin and paclitaxel co-treatment also increased miR-148a levels and regulated the levels of its target genes MSK1 and IRS1. CONCLUSION Curcumin may restore paclitaxel sensitivity by raising miR-148a expression and inhibiting its target genes.
Exploring the complex a

Petrology of blueschist from the Western Himalaya (Ladakh, NW India): Exploring the complex behavior of a lawsonitebearing system in a paleo-accretionary setting / Groppo, Chiara; Rolfo, Franco; Sachan, Himanshu K. ; Rai, Santosh K. In: LITHOS. ISSN 0024-4937. 252-253(2016), pp. 41-56. Original Citation: Petrology of blueschist from the Western Himalaya (Ladakh, NW India): Exploring the complex behavior of a lawsonite-bearing system in a paleo-accretionary setting
Big data & analytics in healthcare industry

C of uterine cervix is one of the most common malignancies in women worldwide. Early detection of this malignancy is its greatest value, as it can be completely cured if diagnosed at early stages. Micro RNAs are small non-coding RNAs. Mir-21 has been demonstrated to be present in many types of cancer cells, and previous studies have shown that it is overexpressed in cervical cancer tissue. The purpose of this study was to identify Mir-21 in the plasma of patients with cancer of cervix, in order to find a sensitive and cost effective method for detection of cancer of cervix at early stages. Plasma samples of 32 patients and 10 healthy volunteers were collected and RNA was extracted. The presence of Mir-21 in the samples was checked by Real Time PCR. Primers were designed by stem-loop PCR technique. Presence of Mir-21 in the plasma was evaluated by qualification PCR and RT-Real Time PCR, which was used to measure expression of Mir-21 in plasma. These results provide clinical evidence that Mir-21 can be found in the plasma of patients in different stages of cancer of cervix. While it cannot be found in the plasma of healthy individuals, our study showed that in patients with cancer of cervix, Mir-21 can be detected during, and after the completion of the treatment. Our results showed that Mir-21 was expressed in SCC of cervix and the level of its expression correlated with the stage.
The Effect of Competence on Teaching Quality in Yemeni Universities

: Previous literature leaves questions about whether students' perspectives have a role in assessing teaching quality. Few studies of teaching quality have answered whether students' perspectives affect or improve teaching quality in higher education institutes. This study used competence as an extension of a Self-determination Theory to examine the quality of teaching in Yemeni universities. The respondents were between 18 and 26 years of age who were currently pursuing their studies in four Yemeni Universities. Data were collected through self-administered survey questionnaires and analyzed using SPSS version 22.0. A total of 500 sets of questionnaires were distributed, and 415 questionnaires were returned and used for coding, analysis, and hypothesis testing. The findings showed that competence positively influenced teaching quality. The results also demonstrated that the hypothesized model was fit. The perceptions of university staff and professionals concerning their level of satisfaction were suggested for future research.
SOCIALLY LEARNING TO THINK DIVERGENTLY

This study aimed to discover whether 2-year-olds can socially learn to think divergently. Two-year-olds (N=22) who saw an experimenter model a high level of divergent thinking on the Unusual Box Test (modeling 25 different actions, once each) went on to demonstrate a higher level of divergent thinking themselves than (N=22) children who saw a low level of modeling (5 different actions, each), where divergent thinking was measured by the number of different actions children produced that had not been modeled by the experimenter. Additionally, all children in both High and Low Divergence conditions had higher divergent thinking than imitation scores, where imitation involved copying the experimenter’s previous actions. This is the first experiment to show that 2-year-olds’ divergent thinking can be increased, and that 2-year-olds do so by socially learning to think more divergently.
as Entrepreneurial

In the debate on why firms exist, the question of who chooses between firms and markets and on what basis is rarely addressed. This paper argues that the choice is a core element of the entrepreneurial pursuit of visions or conceptions of business opportunities. To successfully organize resources into the envisioned businesses – be it via firms or markets – resource owners must be coordinated on the entrepreneur’s conception of the business and be motivated to perform properly. To solve the dual problem, the organizational form of the firm offers the entrepreneur unique advantages not feasible under the organizational form of markets. Managerial monitoring of the employees, based on reward and punishment, fosters
The Effect of Coenzyme Q10 Supplementation on Vascular Endothelial Growth Factor and Serum Levels of Interleukin 6 and 8 in Women with Breast Cancer: A Double-Blind, Placebo-Controlled, Randomized Clinical Trial and Clinical Risk Management

Background: To better evaluate the ef ﬁ cacy of CoQ10 on the in ﬂ ammatory markers in breast cancer patients, we conducted a clinical study of patients with breast cancer undergoing tamoxifen therapy. CoQ10 serves as an antioxidant and inhibits oxidation caused by reactive oxygen species. The aim of the current study was to assess the effect of coenzyme Q10 supplementation on serum levels of interleukin 6, 8, and vascular endothelial growth factor (VEGF) in patients with breast cancer undergoing tamoxifen therapy by a double-blind, placebo-controlled, randomized clinical trial. Methods: In the study, 30 breast cancer patients and 29 healthy subjects were randomized into four groups. Two groups of intervention received 100 mg CoQ10, and two control groups took placebo once a day for 2 months. Blood draws were obtained at baseline and at the end of the study. Serum levels of IL-6, IL-8 and VEGF were analyzed using ELISA kits. Results: The data of the 59 participants were analyzed. Supplementation with CoQ10 demonstrated a signi ﬁ cant decrease in IL-8 and IL-6 serum levels compared to placebo (P< 0.05). Although the downward trend was evident, CoQ10 supplementation did not reveal any signi ﬁ cant effect on serum VEGF concentration. The group of patients who received supplements showed the most reduction in serum levels of cytokines among other groups. Conclusion: CoQ10 supplementation could be effective in ameliorating in ﬂ ammatory cytokine levels, thereby reducing the consequences of in ﬂ ammation caused by breast cancer. To generalize the results, larger and longer intervention studies with higher safe doses are needed and should take account of possible costs and harms as well as bene ﬁ ts (registration number: IRCT2015042021874N1).
quantification biomechanical under

72 Background: The pia arachnoid complex (PAC) is a cerebrospinal fluid-filled tissue conglomerate 73 that surrounds the brain and spinal cord. Pia mater adheres directly to the surface of the brain while 74 the arachnoid mater adheres to the deep surface of the dura mater. Collagen fibers, known as 75 subarachnoid trabeculae (SAT) fibers, and microvascular structure lie intermediately to the pia and 76 arachnoid meninges. Due to its structural role, alterations to the biomechanical properties of the 77 PAC may change surface stress loading in traumatic brain injury (TBI) caused by sub-concussive 78 hits. The aim of this study was to quantify the mechanical and morphological properties of ovine Methods: Ovine brain samples (n=10) were removed from the skull and tissue was harvested 81 within 30 minutes post-mortem. To access the PAC, ovine skulls were split medially from the 82 occipital region down the nasal bone on the superior and inferior aspects of the skull. A template 83 was used to remove arachnoid samples from the left and right sides of the frontal and occipital 84 regions of the brain. 10 ex-vivo samples were tested with uniaxial tension at 2 mm s -1 , average 85 strain rate of 0.59 s -1 , until failure at <5 hours post extraction. The force and displacement data 86 were acquired at 100 Hz using LabVIEW. PAC tissue collagen fiber microstructure was 87 characterized using second-harmonic generation (SHG) imaging on a subset of n=4 stained tissue 88 samples. To differentiate transverse blood vessels from SAT by visualization of cell nuclei and 89 endothelial cells, samples were stained with DAPI and anti-von Willebrand Factor, respectively. 90 The Mooney-Rivlin model for average stress-strain curve fit was used to model PAC material 91 properties. 92 Results: The elastic modulus, ultimate stress, and ultimate strain were found to be 7.7±3.0 MPa, 93 2.7±0.76 MPa, and 0.60±0.13, respectively. No statistical significance was found across brain dissection locations in terms of biomechanical properties. SHG images were post-processed to obtain average SAT fiber intersection density, concentration, porosity, tortuosity, segment length, 96 orientation, radial counts, and diameter as 0.23%, 26.14%, 73.86%, 1.07±0.28, 17.33±15.25 µm, 97 84.66±49. 18º, 8.15%, 3.46±1.62 µm, respectively. 98 Conclusion: For the sizes, strain, and strain rates tested, our results suggest that ovine PAC 99 mechanical behavior is isotropic, and that the Mooney-Rivlin model is an appropriate curve-fitting 100 constitutive equation for obtaining material parameters of PAC tissues.
Deforestation in Watershed Area; Case of Jompi Watershed Area of Indonesia

— Largeness of deforestation area in Indonesia tends to increase very year, and this phenomenal occurs in forest area of Jompi watershed in Indonesia. This research aimed at (1) identifying the perpetrators of deforestation in Jompi watershed area, (2) knowing the land largeness of the society within the forest area of Jompi watershed, and (3) knowing the factors influencing land largeness of the society in the aforementioned area. The results of this research showed that (1) the agents of deforestation in forest area of Jompi watershed comprised people who worked as farmers and not farmers. The people who worked as not farmers, i.e. : merchants, civil servants, and retired civil servants, carpenter/bricklayer, and drivers; (2) the land largeness of the society on the researched area was about 0.08-0.75 ha per person with the average land largeness was 0.34 ha per person; (3) largeness of deforestation in forest area of Jompi watershed was influenced by household income per capita, the number of family members, lack of knowledge about the function of forest, land largeness outside the forest area of Jompi watershed, transportation expenses to the nearest market, distance of house to the forest, and side job.
of KLF3 results in a mild compensated anemia

The CACCC-binding protein KLF3/BKLF represses a subset of KLF1/EKLF target genes 1 and is required for proper erythroid maturation in vivo . Abstract 32 The CACCC-box binding protein Erythroid Krüppel-like Factor (EKLF/KLF1) is a master 33 regulator that directs the expression of many important erythroid genes. We have previously 34 shown that EKLF drives transcription of a second Klf , Basic Krüppel-like Factor/Klf3 . We have 35 now tested the in vivo role of KLF3 in erythroid cells by examining Klf3 knockout mice. KLF3 36 deficient adults exhibit a mild compensated anemia, including enlarged spleens, increased red 37 pulp, a higher percentage of erythroid progenitors, together with elevated reticulocytes and 38 abnormal erythrocytes in the peripheral blood. Impaired erythroid maturation is also observed in 39 the fetal liver. We have found that KLF3 levels rise as erythroid cells mature to become 40 TER119 + . Consistent with this, microarray analysis of both TER119 - and TER119 + erythroid 41 populations revealed that KLF3 is most critical at the later stages of erythroid maturation and is 42 indeed primarily a transcriptional repressor. Notably, many of the genes repressed by KLF3 are 43 also known to be activated by EKLF. However, the majority of these are not currently 44 recognized as erythroid-specific genes. These results reveal the molecular and physiological 45 function of KLF3, defining it as a feedback repressor that counters the activity of EKLF at 46 selected target genes to achieve normal erythropoiesis. 47
Search for the highly suppressed decays B- -&gt; K+pi(-)pi(-) and B- -&gt; K-K-pi(+)

We report a search for the decays B (cid:1) ! K þ (cid:1) (cid:1) (cid:1) (cid:1) and B (cid:1) ! K (cid:1) K (cid:1) (cid:1) þ , which are highly suppressed in the standard model. Using a sample of ð 467 (cid:2) 5 Þ (cid:3) 10 6 B (cid:1) B pairs collected with the BABAR detector, we do not see any evidence of these decays and determine 90% conﬁdence level upper limits of B ð B (cid:1) ! K þ (cid:1) (cid:1) (cid:1) (cid:1) Þ < 9 : 5 (cid:3) 10 (cid:1) 7 and B ð B (cid:1) ! K (cid:1) K (cid:1) (cid:1) þ Þ < 1 : 6 (cid:3) 10 (cid:1) 7 on the corresponding branching fractions, including systematic uncertainties.
Lower Urinary Tract Dysfunction in Parkinson's Disease: Changes

While we agree that the SF-36 can provide valuable information on the impact of PD, we would argue that the SF-36 may not be suitable as a self-report measure for older adults or PD patients. We have employed the SF-36 in two postal surveys with PD and stroke patients and we have consistently found an unacceptable level of missing information in returned questionnaires of around 20%. The missing items in the SF-36 were concentrated on questions relating to vigorous activities or work. Hayes et al. [2] similarly found in their study with elderly patients that omissions in the SF-36 were concentrated in these questions and detected an agerelated increase of omissions for those aged 75 years or more. Jenkinson et al. do not mention in their study if their respondents' questionnaires had any missing information nor did they indicate if any support or help was available for those with visual or writing difficulties.
Race in

: The article aims to (re) think education for race relations in times that turn out to be gloomy from the film “Tabu”. The work is an exploratory research based on a qualitative reflective approach. The results show that despite the policies that have been seeking to institute new ways of looking at and working on differences, they come up against the idea of a homogeneous national identity that disqualifies differences, preventing the specificities of the black condition in society from being given due importance. We know that we have a long way to go in creating and / or valuing other ways of acting in education, in addition to the attempts to develop an impartial, neutral and politically correct discourse. It is education being called to position itself.
PersistenceDisease in a Murine Model of Virus Protection Against CTL Escape and Clinical

CTL escape mutations have been identiﬁed in several chronic infections, including mice infected with mouse hepatitis virus strain JHM. One outstanding question in understanding CTL escape is whether a CD8 T cell response to two or more immunodominant CTL epitopes would prevent CTL escape. Although CTL escape at multiple epitopes seems intuitively unlikely, CTL escape at multiple CD8 T cell epitopes has been documented in some chronically infected individual animals. To resolve this apparent contradiction, we engineered a recombinant variant of JHM that expressed the well-characterized gp33 epitope of lymphocytic choriomeningitis virus, an epitope with high functional avidity. The results show that the presence of a host response to this second epitope protected mice against CTL escape at the immunodominant JHM-speciﬁc CD8 T cell epitope, the persistence of infectious virus, and the development of clinical disease. The Journal of Immunology, 2003, 171: 2006–2013.
2016). Is 2D Unlabeled Data Adequate for Recognizing Facial Expressions? IEEE Intelligent Systems , 31 (3)

 Abstract — Automatic facial expression recognition is one of the important challenges for computer vision and machine learning. Despite the fact that many successes have been achieved in the recent years, several important but unresolved problems still remain. This paper describes a facial expression recognition system based on the random forest technique. Contrary to the many previous methods, the proposed system uses only very simple landmark features, with the view of a possible real-time implementation on low-cost portable devices. Both supervised and unsupervised variants of the method are presented. However, the main objective of the paper is to provide some quantitative experimental evidence behind more fundamental questions in facial articulation analysis, namely the relative significance of 3D information as oppose to 2D data only and importance of the labelled training data in the supervised learning as opposed to the unsupervised learning. The comprehensive experiments are performed on the BU-3DFE facial expression database. These experiments not only show the effectiveness of the described methods but also demonstrate that the common assumptions about facial expression recognition are debatable.
Probing Septin Function Through Interaction Screens: Identification of Novel Septins and Possible Regulatory Mechanisms

Septins are a family of guanine nucleotide-binding proteins that function in eukaryotic cell division, where they form a high-order cortical structure at the site of division, which is essential in most eukaryotes. Expanded roles have evolved for septins in metazoans, where they also have essential functions in terminally-differentiated cell types, such as neurons and spermatozoa. Specific details of septin function are lacking in most roles described, due at least in part to the limited number of characterized binding partners. In this work, yeast two-hybrid screens and pull-downs from tissue homogenate were used to identify novel septin binding partners for subsequent characterization. The neuron-enriched septin, SEPT5, interacted directly with SUMO E3 ligases of the PIAS family. However, I was not able to demonstrate endogenous sumoylation of SEPT5 and SUMO isoforms did not concentrate with the septins during cytokinesis. SEPT5 also interacted with a novel septin, SEPT12, which I further characterized to be testis-specific and localized to the annulus in mature spermatozoa. Further, using SEPT12-specific reagents, I determined that the annulus forms via sequestration and subsequent segregation from the Golgi during spermiogenesis. SEPT9 pull-downs identified another novel testis-specific septin, SEPT14.
Modeling Network Evolution by Colored Petri Nets

Discovering how information was distributed was essential for tracking, optimizing, and controlling networks. In this paper, a premier approach to analyze the reciprocity of user behavior, content, network structure, and interaction rules to the interplay between information diffusion and network evolution was proposed. Parameterization and insight diffusion patterns were characterized based on the community structure of the underlying network using diffusion related behavior data, collected by a developed questionnaire. The user roles in creating the flow of information were stochastically modeled and simulated by Colored Petri Nets, where the growth and evolution of the network structure was substantiated through the formation of the clustering coefficient, the average path length, and the degree distribution. This analytical model could be used for various tasks, including predicting future user activities, monitoring traffic patterns of networks, and forecasting the distribution of content.