Miracle-dz/UniFAR-FTUs huggingface dataset:
Tailoring Health-promoting Programs for Patients with Chronic Kidney Disease: Randomized Controlled Trial

Research on dietary and lifestyle modifications to decrease cardiovascular risk and slow disease progression has been limited to patients in the later stages of chronic kidney disease (CKD). Studies on the effectiveness of stage-of-change-tailored interventions on lifestyle modifications for individuals with early stage CKD are limited. Using random assignment, 60 patients with early stage CKD who received up to six tailored intervention visits over 30 months were compared to 60 usual care patients on physical indicators, lifestyle behaviors, and quality of life. Tailored interventions were consistent with the trans-theoretical Model of Change. Waist circumference, nutrition, and stress management improved over time in the intervention group. There was no difference or change in quality of life. To promote a healthier lifestyle, findings suggest that clinicians working with patients with CKD should consider patients’ readiness to change their behaviors as well as implementation strategies tailored for different processes of change.
Syk inhibitor attenuates inflammation in lupus mice from FcgRIIb deficiency but not in pristane induction: the influence of lupus pathogenesis on the therapeutic effect

Macrophages are responsible for the recognition of pathogen molecules. The downstream signalling of the innate immune responses against pathogen molecules, lipopolysaccharide (LPS) and (1→3)-β-D-glucan (BG), and the adaptive immune response to antibodies, Fc gamma receptor (FcgR), is spleen tyrosine kinase (Syk). Because pathogen molecules and antibodies could be presented in lupus, impact of Syk and macrophages in lupus is explored. FcgR-IIb deficient (FcgRIIb-/-) mice, a model of inhibitory signalling loss, at 40 weeks old, but not pristane mice (a chemical induction lupus model) demonstrated spontaneous elevation of LPS and BG in serum from gut translocation despite the similarity in faecal microbiome analysis. Syk abundance in FcgRIIb–/– mice was higher than in pristane mice, possibly due to several Syk activators (anti-dsDNA, LPS and BG), and Syk inhibitor–attenuated proteinuria and serum cytokines only in FcgRIIb–/– mice. In addition, LPS + BG enhanced the expression of activating FcgRs, NF-κB and Syk, together with supernatant TNF-α predominantly in FcgRIIb–/– compared to wild-type macrophages. The inhibitors against Dectin-1, Syk and nuclear factor kappa B, but not anti-Raf-1, reduced supernatant TNF-α in LPS+BG-activated macrophages, implying Syk-dependent signalling. The pathogen molecules enhanced activating-FcgRs, without inhibition, through Syk, a shared downstream innate and adaptive signalling, is responsible for the hyper-responsiveness in FcgRIIb–/– macrophages. In conclusion, Syk inhibitor attenuated inflammation in FcgRIIb–/– but not in pristane mice, implying the influence of a lupus genetic background in treatment modalities.
Time‐dependent changes in proliferation, DNA damage and clock gene expression in hepatocellular carcinoma and healthy liver of a transgenic mouse model

Hepatocellular carcinoma (HCC) is highly resistant to anticancer therapy and novel therapeutic strategies are needed. Chronotherapy may become a promising approach because it may improve the efficacy of antimitotic radiation and chemotherapy by considering timing of treatment. To date little is known about time‐of‐day dependent changes of proliferation and DNA damage in HCC. Using transgenic c‐myc/transforming growth factor (TGFα) mice as HCC animal model, we immunohistochemically demonstrated Ki67 as marker for proliferation and γ‐H2AX as marker for DNA damage in HCC and surrounding healthy liver (HL). Core clock genes (Per1, Per2, Cry1, Cry2, Bmal 1, Rev‐erbα and Clock) were examined by qPCR. Data were obtained from samples collected ex vivo at four different time points and from organotypic slice cultures (OSC). Significant differences were found between HCC and HL. In HCC, the number of Ki67 immunoreactive cells showed two peaks (ex vivo: ZT06 middle of day and ZT18 middle of night; OSC: CT04 and CT16). In ex vivo samples, the number of γ‐H2AX positive cells in HCC peaked at ZT18 (middle of the night), while in OSC their number remained high during subjective day and night. In both HCC and HL, clock gene expression showed a time‐of‐day dependent expression ex vivo but no changes in OSC. The expression of Per2 and Cry1 was significantly lower in HCC than in HL. Our data support the concept of chronotherapy of HCC. OSC may become useful to test novel cancer therapies.
Skeletal deformities in elasmobranchs from Australian waters

Four shark species in Australian waters were found with skeletal deformities. Deformities were similar across three species with large scoliotic, lordotic and kyphotic bends of the Vertebral columns. The cause of these deformities is currently unknown. (C) 1999 The Fisheries society of the British Isles.
Elevated antibody titer against Borrelia burgdorferi in new case of multiple sclerosis patients

Multiple sclerosis (MS) is a chronic, inflammatory, demyelinating disease that affects central nervous system (CNS). The etiology of MS is unknown, but it likely involves a combination of genetic, nongenetic and environmental factors such as microbial agents. In this descriptive case- control study, 61 new cases of MS patients and 60 healthy controls were studied for IgM and IgG antibody titer to Borrelia burgdorferi . The samples were tested for the presence of specific antibodies by ELISA method. The data were analyzed using descriptive statistics. The results showed that specific IgM antibody titer against B. burgdorferi were significantly higher in patient group than control (OR=4.323, 95% CI=1-6.4, p=0.001). Our results confirm the association between B. burgdorferi and MS in Iranian patients.
Innovation in Later Life: A Study of Grandmothers and Facebook

Relying on the Innovation theory of successful aging, this study aims at exploring how grandmothers around the world perceive and use Facebook. Twenty-seven focus groups were set up in seven countries: Canada, Colombia, Israel, Italy, Peru, Romania, and Spain. Participants consisted of 184 grandmothers aged 65 and over who use information and communication technology to some extent. Data were analyzed according to hybrid thematic analysis principles and findings pointed to substantial ambivalence regarding Facebook. Grandmothers who used it reported rather restrained use that was typically triggered externally and characterized by reactive use patterns. Nevertheless, Facebook appeared to promote users’ well-being by supporting preservation of their relationships, roots, and sense of relevance. These findings suggest a new theoretical tenet to innovation theory according to which even “restrained innovation” in later life may be beneficial.
Identification of a defective molecule derived from DNA‐A of the bipartite begomovirus of East African cassava mosaic virus

Geminivirus defective interfering DNAs arise spontaneously in mechanically inoculated test plants, and have previously been found with DNA-B of the bipartite cassava mosaic geminiviruses, but not DNA-A. Reported here for the first time is the cloning and characterization of a naturally occurring truncated form of cassava mosaic geminivirus DNA-A, which at 1525 nt is around half the expected full size. Sequence analysis has shown it to be a defective (df) form of East African cassava mosaic virus (EACMV) DNA-A that has retained its cis elements essential for replication by the helper virus, and it has been termed df DNA-A 15. Phylogenetic comparisons placed the df DNA-A 15 molecule close to mild and severe isolates of EACMV-UG2. Biolistic inoculation of Nicotiana benthamiana with infectious df DNA-A 15 clone and East African cassava mosaic Cameroon virus (EACMCV) resulted in symptom amelioration as compared with EACMCV singly inoculated plants, and there was an accumulation of df DNA-A 15 in systemically infected leaves. In addition, the level of EACMV DNA-B accumulation was reduced in the coinoculated plants compared with those inoculated with EACMCV alone. PCR and sequence analysis confirmed the helper virus as EACMV.
Gender differences in the prosecution of police assault: Evidence from a natural experiment in Sweden

States often seek to regulate the use of police force though citizen complaint systems. This paper examines these systems, specifically, whether patterns of bias found in other juridical contexts are mirrored in the adjudication of police assault. The analysis focuses on prosecutors as the first instance of adjudication who determine whether to move forward with investigation, effectively deciding the majority of cases. We ask whether prosecutor sex is associated with the probability that a police assault claim will be investigated. We leverage a natural experiment in Sweden where prosecutors are assigned through a modified lottery system, effectively randomizing appointment. Our findings suggest that prosecutor gender plays a role in judicial outcomes: women prosecutors are 16 percentage points more likely to investigate claims of police assault than their male counterparts. These findings have implications for scholars interested in state human rights abuses, democratic institutions, and judicial inequality.
The neural basis of metacognitive monitoring during arithmetic in the developing brain

In contrast to a substantial body of research on the neural basis of cognitive performance in several academic domains, less is known about how the brain generates metacognitive (MC) awareness of such performance. The existing work on the neurobiological underpinnings of metacognition has almost exclusively been done in adults and has largely focused on lower level cognitive processing domains, such as perceptual decision‐making. Extending this body of evidence, we investigated MC monitoring by asking children to solve arithmetic problems, an educationally relevant higher‐order process, while providing concurrent MC reports during fMRI acquisition. Results are reported on 50 primary school children aged 9–10 years old. The current study is the first to demonstrate that brain activity during MC monitoring, relative to the control task, increased in the left inferior frontal gyrus in children. This brain activity further correlated with children's arithmetic development over a 3‐year time period. These data are in line with the frequently suggested, yet never empirically tested, hypothesis that activity in the prefrontal cortex during arithmetic is related to the higher‐order process of MC monitoring.
Towards the flower economics spectrum.

Understanding how floral traits affect reproduction is key for understanding genetic diversity, speciation, and trait evolution in the face of global changes and pollinator decline. However, there has not yet been a unified framework to characterize the major trade-offs and axes of floral trait variation. Here, we propose the development of a floral economics spectrum (FES) that incorporates the multiple pathways by which floral traits can be shaped by multiple agents of selection acting on multiple flower functions. For example, while pollinator-mediated selection has been considered the primary factor affecting flower evolution, selection by non-pollinator agents can reinforce or oppose pollinator selection, and, therefore, affect floral trait variation. In addition to pollinators, the FES should consider non-pollinator biotic agents and floral physiological costs, broadening the drivers of floral traits beyond pollinators. We discuss how coordinated evolution and trade-offs among floral traits and between floral and vegetative traits may influence the distribution of floral traits across biomes and lineages, thereby influencing organismal evolution and community assembly.
Fibrinogen - Cellular Prion Protein Complex Formation on Astrocytes.

Traumatic brain injury (TBI) is one of the most common neurological disorders causing memory reduction, particularly short-term memory (STM). We showed that during TBI-induced inflammation, increased blood content of fibrinogen (Fg) enhanced vascular protein transcytosis and deposition of extravasated Fg in vasculo-astrocyte interfaces. In addition, we found that deposition of cellular prion protein (PrPC) was also increased in the vasculo-astrocyte endfeet interface. However, association of Fg and PrPC was not confirmed. Presently, we aimed to define whether Fg can associate with PrPC on astrocytes and cause their activation. Cultured mouse brain astrocytes MBA were treated with medium alone (control), Fg (2 mg/ml or 4 mg/ml), 4 mg/ml of Fg in the presence of a function-blocking anti-PrPC peptide or anti-mouse IgG, function-blocking anti-PrPC peptide, or anti-mouse IgG alone. After treatment, either cell lysates were collected and analyzed via Western blot or co-immunoprecipitation was performed, or astrocytes were fixed and their activation was assessed with immunohistochemistry. Results showed that Fg dose-dependently activated astrocytes, increased expressions of PrPC and tyrosine (tropomyosin) receptor kinase B (TrkB), and PrP gene. Blocking the function of PrPC reduced these effects. Co-immunoprecipitation demonstrated Fg and PrPC association. Since it is known that prion protein has a greater effect on memory reduction than amyloid beta, and that activation of TrkB is involved in neurodegeneration, our findings confirming the possible formation of Fg-PrPC and Fg-induced overexpression of TrkB on astrocytes suggest a possible triggering mechanism for STM reduction that was seen previously during mild-to-moderate TBI.
Biofilms as major sources of Legionella spp. in hydrothermal areas and their dispersion into stream water

Water and biofilms from two hydrothermal areas in central Portugal, and one hydrothermal area in New Mexico, USA, were examined for Legionella spp. In general, Legionella spp were isolated in higher numbers from biofilms than from water, although one biofilm with a temperature of 50°C, did not yield isolates of these organisms. In one area L. pneumophila serogroup (sg) 3 constituted the major population in the thermal discharge by the stream and the biofilm below it; however, L. pneumophila sg 1 was predominant in the sediments of the stream bed with minor thermal springs below the main discharge and in the water downstream. No Legionellae were isolated from water upstream of the hydrothermal area indicating that the thermal area was the source of the organisms in the stream water. In the other two hydrothermal areas, L. pneumophila sg 1 constituted the major population isolated, whereas L. pneumophila sg 3 was absent or isolated in low numbers. Isolates of L. micdadei were also recovered from one hydrothermal area, while ‘L. londoniensis’ was isolated from another.
COVID 19: challenges for virologists in the food industry

The COVID‐19 pandemic is not only a challenge for public health and hospitals, but affects many aspects of our societies. This Lilliput minireview deals with problems that the pandemic causes for the food industry, addressing the presence and persistence of SARS‐CoV‐2 in the food environment, methods of virus inactivation and the protection of the food worker and the consumer. So far food has not been implicated in the transmission of the infection, but social disruptions caused by the pandemic could cause problems with food security.
Qualitative and Semi-Quantitative Assessment of Processing-Related Surface Contamination of One- and Two-Piece CAD/CAM Abutments before and after Ultrasonic Cleaning

Manufacturing processes of custom implant abutments may contaminate their surfaces with micro wear deposits and generic pollutants. Such particulate debris, if not removed, might be detrimental and provoke inflammatory reactions in peri-implant tissues. Although regulatory guidelines for adequate cleaning, disinfection, or sterilization exist, there does not appear to be a consistent application and data on the amount and extent of such contaminants is lacking. The aim of the present in vitro study was to evaluate the quality and quantity of processing-related surface contamination of computer-aided design/computer-aided manufacturing (CAD/CAM) abutments in the state of delivery and after ultrasonic cleaning. A total of 28 CAD/CAM monotype and hybrid abutments were cleaned and disinfected applying a three-stage ultrasonic protocol (Finevo protocol). Before and after cleaning, the chemical composition and the contamination of the abutments were assessed using scanning electron microscopy (SEM), dispersive X-ray spectroscopy (EDX), and computer-aided planimetric measurement (CAPM). In the delivery condition, monotype abutments showed a significantly higher amount of debris compared to hybrid abutments (4.86 ± 6.10% vs. 0.03 ± 0.03%, p < 0.001). The polishing process applied in the laboratory after bonding the hybrid abutment components reduces the surface roughness and thus contributes substantially to their purity. The extent of contamination caused by computer-aided manufacturing of custom abutments can be substantially minimized using a three-stage ultrasonic protocol.
Antioxidant and Anti-Inflammatory Effects of NCW Peptide from Clam Worm (Marphysa sanguinea)

Clam worms (Marphysa sanguinea) are a rich source of bioactive components such as the antibacterial peptide, perinerin. In the present study, we explored the physiological activities of a novel NCWPFQGVPLGFQAPP peptide (NCW peptide), which was purified from clam worm extract through high-performance liquid chromatography. Tandem mass spectrometry (MS/MS) revealed that NCW was a new peptide with a molecular weight of 1757.86 kDa. Moreover, NCW peptide exhibited significant antioxidant effects, causing a 50% inhibition of DPPH radical at a concentration of 20 μM without showing any cytotoxicity. These were associated with a reduction in the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in LPS-stimulated RAW264. 7 cells. Furthermore, NCW peptide exhibited anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via inhibition of the abnormal production of pro-inflammatory cytokines including nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). These anti-inflammatory effects of NCW peptide were associated with the inhibition of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Our results therefore suggest that this novel NCW peptide with antioxidant and anti-inflammatory effects could be a good therapeutic agent against inflammation-related diseases.
Reply to: Bergmann et al comment to “Patients who report cording after breast cancer surgery are at higher risk of lymphedema: Results from a large prospective screening cohort”

Dear Dr. Sener, We would like to thank Bergmann et al for their detailed review of our manuscript entitled “Patients who report cording after breast cancer surgery are at higher risk of lymphedema: Results from a large prospective screening cohort. ” We both share an interest in better understanding the relationship between axillary web syndrome (AWS) and breast cancer‐related lymphedema (BCRL), which profoundly impact patients treated for breast cancer. We agree with the authors that studies have found contradictory results regarding the effect of AWS on BCRL risk. In part, these contradictory results may be caused by the lack of standardization of measurement for BCRL across studies. The authors note that three publications have found no relevant relationship between AWS and BCRL2‐4; however, two of these studies did not examine the relationship between AWS and BCRL in any case, as study follow‐up was only up to 4 and 12 weeks postoperatively respectively, reflecting early postoperative volume changes, not BCRL. Koehler et al's group did go on to publish a manuscript with 18 month follow‐up, citing a lack of association between AWS and BCRL; however, this study used absolute volume and circumferential differences between arms, and it did not incorporate baseline measures. Using absolute criteria (such as 200mL difference between arms) does not account for arm asymmetry at preoperative baseline and will lead to wide variability in terms of relative volume change. Lack of incorporation of a preoperative baseline measurement into BCRL diagnosis accounts for misdiagnosis rates up to 50%. The third study cited by Bergmann et al was completed by their group and did not find a relationship between AWS and BCRL. Their study differs from ours in terms of breast cancer treatment, BC risk in the cohort (eg, BCRL risk factors including axillary node dissection [ALND], elevated body mass index [BMI], and lack of breast reconstruction), study methodology, and statistical methods (Table 1). These differences may account for differences in study outcomes. Finally, the methodology for BCRL measurement in Wariss et al's study is inaccurate. They defined lymphedema as an absolute arm volume difference of more than 200mL, as calculated from girth measurements, without incorporation of preoperative baseline. We recognize that at the time of data collection, 200mL difference
Few Do and To Few: Disclosure of Suicidal Thoughts in Friendship Networks of Young Adults Experiencing Homelessness

Abstract Disclosure of one’s suicidal thoughts is a pivotal but under-investigated mechanism for preventing suicide among young adults experiencing homelessness (YAEH). In a sample of 527 YAEH, we adopted a multi-level perspective to assess patterns and correlates of disclosure in their friendship networks. Less than one-third of YAEH disclosed their suicidal thoughts—half of them doing so during a suicidal crisis—and only disclosed to 21% of their friends. Multilevel modeling showed that YAEH who reported a history of unmet mental health needs were more likely to have disclosed to a friend, and friends who were sources of social support were most highly sought out for disclosures. Our findings highlight the need for cultivating safe environments that promote disclosures among YAEH.
Profiling of phenolic and other compounds from Egyptian cultivars of chickpea (Cicer arietinum L.) and antioxidant activity: a comparative study

Chickpeas are basic food in many countries with several cultivars distributed all over the world. However, little is known about their secondary metabolites. Thus, this work is focused on the study of the phenolic profiles of seven Egyptian cultivars of chickpea. Selecting the most appropriate extraction method and analytical conditions using reversed-phase high-performance liquid chromatography-diode array detection, with a core–shell column, and coupled with quadrupole-time-of-flight-mass spectrometry (MS), a total of 96 phenolic compounds were characterized based on their retention time, UV spectra, and accurate MS and MS2 data. Among them, the major phenolic subclasses were hydroxybenzoic acids and flavonoids. Moreover, other minor and major metabolites including organic acids, amino acids, nucleosides, peptides and soyasaponins were characterized. Using standards, 22 compounds were unequivocally identified. Remarkably, 88 of these compounds were tentatively reported for the first time in chickpeas. The total phenol content of the cultivars was determined as well as the antioxidant activity by the trolox equivalent antioxidant capacity assay.
Considering the impact of viral illness on the aetiology of clozapine myocarditis

Australian & New Zealand Journal of Psychiatry, 55(2) 18F-florbetaben was arranged, which showed radioligand binding in the cortical regions, indicative of high amyloid burden (Figure 1(E)). A diagnosis of lvPPA was established and cognitive enhancer was added as the patient’s main treatment strategy soon. This case illustrates the complexity of the differential diagnostic processes in young-onset dementia, suggesting that language profile should be taken into account when differentiating lvPPA from bvFTD in patients with behavior symptoms. We also highlight that lvPPA patients could present with neuropsychiatric symptoms and obviously right-sided hemispheric hypoperfusion at the beginning of the illness.