Miracle-dz/UniFAR-FTUs huggingface dataset:
The overexpression of glypican-5 promotes cancer cell migration and is associated with shorter overall survival in non-small cell lung cancer

Although the correlation between glypican-5 (GPC5) and lung cancer is well known, the effect of GPC5 expression on non-small cell lung cancer (NSCLC) survival remains to be determined. In the present study, GPC5 expression in A549, H3255, and SPC-A1 NSCLC cell lines was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. GPC5 mRNA and protein expression levels were found to be higher in A549 and H3255 cells compared with SPC-A1 cells. The role of GPC5 in NSCLC cell migration was evaluated in vitro by shRNA-mediated knockdown or the overexpression of GPC5 through scratch and transwell assays. The mean migration rates of cancer cells transfected with pRNAT-shRNA-GPC5-1 were reduced compared with the controls in A549 (P<0.001) and H3255 (P=0.001), while the migration rate of SPC-A1 with GPC5 overexpression was higher than that of the control (P=0.001). The downregulation of GPC5 impeded the transmigration of A549 and H3255 while the upregulation of GPC5 expression promoted the transmembrane invasion of SPC-A1. Furthermore, a panel of formalin-fixed paraffin-embedded NSCLC tissues from 127 patients undergoing curative resection (stages I, II and III) between January, 2003 and December, 2008 were obtained in order to investigate the correlation between GPC5 expression and clinicopathological factors using immunohistochemical methods. The results demonstrated that high GPC5 expression levels in NSCLC were associated with respiratory symptoms in lung cancer diagnosis, poor differentiation, vascular invasion, regional lymph node metastasis and a higher TNM stage. Using the Kaplan-Meier method, NSCLC patients with high levels of GPC5 expression demonstrated a significantly shorter overall survival time compared with those with low GPC5 expression levels (median postsurgical survival time: 14.0 months vs. 59.0 months, P=0.001). GPC5 expression was also identified as an independent prognostic factor by Cox regression analysis [adjusted hazard ratio: 2.18; 95% confidence interval (CI): 1.35–3.52; P=0.001]. This study suggested that increased levels of GPC5 expression are a poor prognostic marker for NSCLC.
Sympathoexcitation by PVN-Injected Bicuculline Requires Activation of Excitatory Amino Acid Receptors

Abstract—Acute blockade of &ggr;-aminobutyric acid (GABA)-A receptors in the hypothalamic paraventricular nucleus (PVN) increases mean arterial pressure (MAP), heart rate (HR), and sympathetic nerve activity (SNA). However, the underlying neural mechanisms have not been fully determined. We tested the hypothesis that responses to GABA-A receptor blockade in the PVN require activation of local ionotropic excitatory amino acid (EAA) receptors. MAP, HR, and renal SNA responses to unilateral PVN microinjection of bicuculline methobromide (BIC, 0.1 nmol) were recorded before and after ipsilateral PVN injection of either vehicle (saline), the nonselective ionotropic EAA receptor antagonist kynurenate (KYN), the NMDA receptor antagonist D(-)-2-amino-5-phosphonopentanoic acid (AP5), or the non-NMDA receptor antagonist 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium (NBQX). Responses to PVN-injected BIC were unaltered by vehicle injection. In contrast, injection of KYN (7.2 nmol; n=4) nearly abolished ABP and renal SNA responses to BIC (P <0.01) and significantly attenuated (P <0.05) HR responses as well. Similarly, graded doses of AP5 (0.6, 3, and 6 nmol) and NBQX (0.26, 1.3, and 2.6 nmol) reduced responses to PVN-injected BIC in a dose-related manner, with the 3 nmol (n=7) and 1.3 nmol (n=6) doses producing maximal effects (P <0.05). KYN, AP5, and NBQX did not affect baseline parameters. Effects of a cocktail containing AP5 (3 nmol) and NBQX (1.3 nmol) were greater (P <0.01) than either antagonist alone and were not statistically different from KYN. These data indicate that cardiovascular and renal sympathetic responses to acute GABA-A receptor blockade in the PVN require local actions of EAAs at both NMDA and non-NMDA receptors.
A PREFERENCE RANKING MODEL USING A DISCRIMINATIVELY-TRAINED CLASSIFIER

This paper presents an ordinal regression approach to the query-by-description problem. Instead of returning a single classification, such as genre, or a list of the top N songs assumed to be relevant, this algorithm mirrors choices similar to "like", "skip", "play", and "hate" buttons seen on commercial Internet radio stations. Ordinal regression can be viewed as a hybrid between multi-class classification and regression. While ordinal regression defines a small number of discrete classes similar to multi-class classification, there is a dimension of preference which determines the order of classes, which is similar to regression. To model a given song, a transcription of acoustic segment models is derived from an unsupervised approach using hidden Markov models. These transcriptions serve as text documents for latent semantic analysis. A discriminativelytrained ordinal regression algorithm provides the ranks for a given song. Test queries and ground-truth labels are developed using textual musical descriptions from songs to combat the problem that listeners do not base musical similarity solely on acoustic perception. Initial results on a small set of queries indicate the proposed ordinal regression approach performs better than a common perceptron-based ordinal regression algorithm. Further, the results using acoustic segment models is shown to be superior compared to a typical bag-of-frames algorithm using Gaussian mixture models. Extensions to real playlists and user preference indicators are currently in development.
Retail Competition and the Dynamics of Consumer Demand for Tied Goods

We empirically investigate the demand for tied goods sold through competing retail channels. Tied good pricing strategies commonly involve a low price on the initial purchase (i.e. the primary good) to drive adoption, and a substantial markup on aftermarket goods to capture value. However, if the goods are sold through downstream channels, retail market power and a misalignment of incentives could distort the relative prices of primary and aftermarket goods. To evaluate whether retail competition is strong enough to prevent such distortions, we explore the commonly noted example of razors and blades, which are sold through drug, grocery, mass merchandising, and club stores. We specify a forward-looking demand model that incorporates dynamics arising from the tied good nature of the products and the stockpiling and durability aspects of razors and blades. Furthermore, we allow intertemporal substitution in the purchase of both razors and blades to occur across channels as well as time. This modeling feature enables a novel approach to measuring retail competition in single category demand analyses. Our estimates indicate that there is substantial cross-channel substitution in razors, but some retail market power in blades. However, the channel with the most market power in blades, club stores, specializes in high volume customers that would adopt a razor even if blade prices are higher. This suggests that the manufacturer can achieve its desired level of razor adoption without vertical restraints, though blade sales may be slightly reduced by double marginalization.
Subcortical encoding of sound is enhanced in bilinguals and relates to executive function advantages

Bilingualism profoundly affects the brain, yielding functional and structural changes in cortical regions dedicated to language processing and executive function [Crinion J, et al. (2006) Science 312:1537–1540; Kim KHS, et al. (1997) Nature 388:171–174]. Comparatively, musical training, another type of sensory enrichment, translates to expertise in cognitive processing and refined biological processing of sound in both cortical and subcortical structures. Therefore, we asked whether bilingualism can also promote experience-dependent plasticity in subcortical auditory processing. We found that adolescent bilinguals, listening to the speech syllable [da], encoded the stimulus more robustly than age-matched monolinguals. Specifically, bilinguals showed enhanced encoding of the fundamental frequency, a feature known to underlie pitch perception and grouping of auditory objects. This enhancement was associated with executive function advantages. Thus, through experience-related tuning of attention, the bilingual auditory system becomes highly efficient in automatically processing sound. This study provides biological evidence for system-wide neural plasticity in auditory experts that facilitates a tight coupling of sensory and cognitive functions.
Down-regulation of the Neurotrophin Receptor TrkB following Ligand Binding

This study examines the mechanisms by which the tyrosine kinase receptor TrkB is down-regulated following binding of brain-derived neurotrophic factor (BDNF). In primary cultures of cerebellar granule neurons, BDNF-induced reduction of TrkB receptors was largely prevented by the addition of specific proteasome inhibitors. HN10 cells, a neuronal cell line that can be readily transfected, also showed a marked down-regulation of cell surface TrkB following BDNF exposure. In addition, we observed that prolonged exposure to nerve growth factor of TrkA-transfected cells did not lead to the down-regulation seen with BDNF and TrkB. TrkA and TrkB chimeric molecules were therefore expressed in HN10 cells and tested for ligand-induced regulation. These experiments led to the conclusion that the motives responsible for down-regulation are contained in the cytoplasmic domain of TrkB, and a short sequence in the juxtamembrane domain of TrkB was identified that confers nerve growth factor-induced down-regulation when inserted into TrkA.
Context-Adaptive Binary Arithmetic Coding With Fixed-Length Codewords

Context-adaptive binary arithmetic coding is a widespread technique in the field of image and video coding. Most state-of-the-art arithmetic coders produce a (long) codeword of a priori unknown length. Its generation requires a renormalization procedure to permit progressive processing. This paper introduces two arithmetic coders that produce multiple codewords of fixed length. Contrary to the traditional approach, the generation of fixed-length codewords does not require renormalization since the whole interval arithmetic is stored in the coder's internal registers. The proposed coders employ a new context-adaptive mechanism based on variable-size sliding window that estimates with high precision the probability of the symbols coded. Their integration in coding systems is straightforward as demonstrated within the framework of JPEG2000. Experimental tests indicate that the proposed coders are computationally simpler than the MQ coder of JPEG2000 and the M coder of HEVC while achieving superior coding efficiency.
Time-Aware Collaborative Filtering for QoS-Based Service Recommendation

In QoS-based Web service recommendation, predicting QoS(Quality of Service) for service users will greatly aid service selection and discovery. In order to improve the prediction accuracy of Collaborative filtering algorithms, various factors are taken into account (e.g., location factor, environment, etc.). But seldom do investigators take the factor of time into account. Actually, QoS performance of Web services is highly related to the service status and network environments which are variable against time. Thus, this paper proposes a time-aware collaborative filtering algorithm to predict the missing QoS values. To validate our algorithm, this paper conducts series of large-scale experiments based on a real-world Web service QoS dataset. Experimental results show that the time-aware collaborative filtering algorithm significantly improves prediction accuracy.
Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells

Adenylate cyclase toxin (CyaA) is a key virulence factor of the whooping cough agent Bordetella pertussis. The toxin targets CD11b-expressing phagocytes and delivers into their cytosol an adenylyl cyclase (AC) enzyme that subverts cellular signaling by increasing cAMP levels. In the present study, we analyzed the modulatory effects of CyaA on adhesive, migratory and antigen presenting properties of Toll-like receptor (TLR)-activated murine and human dendritic cells (DCs). cAMP signaling of CyaA enhanced TLR-induced dissolution of cell adhesive contacts and migration of DCs towards the lymph node-homing chemokines CCL19 and CCL21 in vitro. Moreover, we examined in detail the capacity of toxin-treated DCs to induce CD4+ and CD8+ T cell responses. Exposure to CyaA decreased the capacity of LPS-stimulated DCs to present soluble protein antigen to CD4+ T cells independently of modulation of co-stimulatory molecules and cytokine production, and enhanced their capacity to promote CD4+CD25+Foxp3+ T regulatory cells in vitro. In addition, CyaA decreased the capacity of LPS-stimulated DCs to induce CD8+ T cell proliferation and limited the induction of IFN-γ producing CD8+ T cells while enhancing IL-10 and IL-17-production. These results indicate that through activation of cAMP signaling, the CyaA may be mobilizing DCs impaired in T cell stimulatory capacity and arrival of such DCs into draining lymph nodes may than contribute to delay and subversion of host immune responses during B. pertussis infection.
Al-Zoning of Serpentine Aggregates in Mesh Texture Induced by Metasomatic Replacement Reactions

Serpentinization of oceanic lithosphere commonly proceeds with the development of mesh texture. Examination of a serpentinized harzburgite and a plagioclase-bearing wehrlite revealed conspicuous zoning of Al in a serpentine mesh texture, with Al-rich cores and Al-poor rims, as well as Al-rich veins, indicating local transport of Al from plagioclase and pyroxene during serpentinization. To reveal the influences of Al on the reaction mechanisms and textural development during serpentinization, we conducted hydrothermal experiments in the olivine (Ol)–plagioclase (Pl)–H2O system and analyzed the variations in mineralogy and microtexture of olivine replacements as a function of distance from the Ol–Pl boundary. The Al–Si metasomatic zone, where Al-serpentine and a minor amount of Ca-saponite were formed, was developed in the Ol-hosted region close to the Ol–Pl boundary. Far from the Ol–Pl boundary, Al-free serpentine, brucite, and magnetite were formed, indicating the progress of an ‘isochemical’ reaction (separate from water). The aggregates of Al-serpentine after olivine in the metasomatic zone showed a characteristic zoning of Al. Microtextural evidence indicates that the zoning was produced in response to the migration of an Al metasomatic front that involved an early-stage of serpentinization with an Al-free solution, and the subsequent pseudomorphic replacement of olivine and simultaneous development of overgrowths as the amount of Al increased in the solution. The Al-bearing aqueous solution caused the formation of olivine pseudomorphs and this contrasts with the lack of preservation of original olivine outlines in the isochemical zones. Comparisons of zoning in natural and experimentally produced mesh textures suggest that Al-poor rims in the mesh texture form at the start of the serpentinization process, followed by the coupled formation of Al-rich mesh cores and Al-rich veins. Our experimental results indicate that Al-zoning in the mesh texture represent the transition from a closed to an open system during serpentinization.
Right ventricular function in the human fetus

Abstract Fetal RV function is critical for survival and normal cardiovascular development. Doppler can be used to assess function. Combined with wise use of imaging techniques, the cause of abnormalities can be assessed. Both congenital heart defects and other cardiovascular defects can lead to hydrops and death. Serial semi-quantiation of fetal heart failure can be done with a Cardiovascular Profile score.
Time-interleaved pipelined ADC design for computational intelligence and cloud computing application

A 10-bit 60-MS/s time-interleaved pipelined analog-to-digital converter (ADC) is successfully designed and implemented. Long term evolution advance (LTE-A) design for computational Intelligence healthcare systems and cloud computing application. Electrocardiogram (ECG) and human body big data transfer are the important physiological parameters in human body. Through monitoring and analyzing ECG and human body big data transfer, we can know the health condition of human body and achieve the purpose of prevention and diagnosis for diseases accordingly.
Transcriptome Analysis of miRNA and mRNA in Porcine Skeletal Muscle following Glaesserella parasuis Challenge

Glaesserella parasuis (G. parasuis) causes systemic infection in pigs, but its effects on skeletal muscle and underlying mechanisms are poorly understood. We investigated G. parasuis infection in colostrum-deprived piglets, observing decreased daily weight gain and upregulation of inflammatory factors in skeletal muscle. Muscle fiber area and diameter were significantly reduced in the treated group (n = 3) compared to the control group (n = 3), accompanied by increased expression of FOXO1, FBXO32, TRIM63, CTSL, and BNIP3. Based on mRNA and microRNA (miRNA) sequencing, we identified 1642 differentially expressed (DE) mRNAs and 19 known DE miRNAs in skeletal muscle tissues between the two groups. We predicted target genes with opposite expression patterns to the 19 miRNAs and found significant enrichment and activation of the FoxO signaling pathway. We found that the upregulated core effectors FOXO1 and FOXO4 were targeted by downregulated ssc-miR-486, ssc-miR-370, ssc-miR-615, and ssc-miR-224. Further investigation showed that their downstream upregulated genes involved in protein degradation were also targeted by the downregulated ssc-miR-370, ssc-miR-615, ssc-miR-194a-5p, and ssc-miR-194b-5p. These findings suggest that G. parasuis infection causes skeletal muscle atrophy in piglets through accelerated protein degradation mediated by the “miRNAs-FOXO1/4” axis, while further research is necessary to validate the regulatory relationships. Our results provide new insights into the understanding of systemic inflammation growth mechanisms caused by G. parasuis and the role of miRNAs in bacterial infection pathogenesis.
Performance Evaluation of LDPC Codes on PLC Channel Compared to Other Coding Schemes

In this paper we study how power line communications are affected by different coding techniques. We investigate the case of LDPC coding scheme and its application to the PLC channel. The modulation method applied is BPSK while the transmission technique used is OFDM. We study regular LDPC codes, which is the worst case scenario concerning the performance of LDPC codes. The OFDM symbol size is initially defined at 4096 carriers, since certain characteristics of LDPC codes, like their sparse parity check matrix, imply that a larger block size improves their performance. In this paper, the coding and decoding technique have firstly been applied to a realistic power line channel. The attenuation caused by the PLC channel is derived from actual measurements. We test the performance of LDPC codes of frac12 code rate, by means of BER, compared to Reed-Solomon codes of similar code rate and convolutional codes, when they are all applied to the PLC channel and under the same conditions. Additionally, impulsive noise is generated and the system's performance is tested under the coding schemes mentioned above. Afterwards, we examine how different code rates affect the BER. All the above results are obtained via computer simulations.
HER2 Testing in Gastric Cancer

Molecular therapies targeting HER2 are part of the established drug armamentarium in breast carcinoma. Now the ToGA trial, an international multicenter phase III clinical study, involving 24 countries globally, has shown that the anti-HER2 humanized monoclonal antibody Trastuzumab is effective in prolonging survival in HER2-positive carcinoma of the stomach and the gastroesophageal junction (GEJ). Similarly to breast carcinoma, >20% of gastric cancers show HER2 overexpression and/or amplification, and this percentage increases to 33% in GEJ tumors. Thus, as in breast carcinoma, pathologists are now asked to evaluate HER2 status in gastric carcinoma samples. As validated in the ToGA trial, the HER2 testing criteria that must be used in evaluating both gastric carcinoma biopsies and surgical specimens significantly differ from those routinely applied in breast carcinoma. The main variations with regard to the pattern of reactivity in HER2-expressing cells are as follows: the completeness of membrane staining is not a “conditio sine qua non” and the number of stained cells necessary to consider a case as positive is different. We must also take note of the much more frequent heterogeneity of HER2 positivity in gastric cancer compared with breast carcinoma and the less stringent correlation between HER2 amplification and protein overexpression that is observed in gastric carcinoma, where more than 20% of cases may carry HER2 amplification, although of low level, without HER2 expression. In these patients, in the ToGA trial, there was no apparent benefit from adding Trastuzumab to chemotherapy: for this reason the European Medicines Agency, while approving usage of Trastuzumab for metastatic adenocarcinoma treatment, indicated HER2 testing by immunohistochemistry as first evaluation assay, followed by fluorescence in situ hybridization in 2+ equivocal cases. HER2 testing in gastric carcinoma is a new field, opening several opportunities: for patients with gastric cancer, this is a new promising therapeutic option; for pathologists, strengthening our role in therapy selection and emphasizing our duty of providing accurate and reproducible HER2 testing results; for all interested in understanding the biology of gastric and GEJ cancer and in discovering new possible molecular therapy targets.
Efficient Qos Based Resource Scheduling Using PAPRIKA Method for Cloud

Cloud computing is increasingly been used in enterprises and business markets for serving demanding jobs. The performance of resource scheduling in cloud computing is important due to the increase in number of users, services and type of services. Resource scheduling is influenced by many factors such as CPU speed, memory, bandwidth etc. Therefore resource scheduling can be modeled as a multi criteria decision making problem. This study proposes an efficient QoS based resource scheduling algorithm using potentially all pair-wise rankings of all possible alternatives (PAPRIKA). The tasks are arranged based on the QoS parameters and the resources are allocated to the appropriate tasks based on PAPRIKA method and user satisfaction. The scheduling algorithm was simulated with cloudsim tool package. The experiment shows that, the algorithm reduces task completion time and improves resource utility rate.