Miracle-dz/UniFAR-FTUs huggingface dataset:
Structural and Biochemical Analysis Reveals Catalytic Mechanism of Fucoidan Lyase from Flavobacterium sp. SA-0082

Fucoidans represent a type of polyanionic fucose-containing sulfated polysaccharides (FCSPs) that are cleaved by fucoidan-degrading enzymes, producing low-molecular-weight fucoidans with multiple biological activities suitable for pharmacological use. Most of the reported fucoidan-degrading enzymes are glycoside hydrolases, which have been well studied for their structures and catalytic mechanisms. Little is known, however, about the rarer fucoidan lyases, primarily due to the lack of structural information. FdlA from Flavobacterium sp. SA-0082 is an endo-type fucoidan-degrading enzyme that cleaves the sulfated fuco-glucuronomannan (SFGM) through a lytic mechanism. Here, we report nine crystal structures of the catalytic N-terminal domain of FdlA (FdlA-NTD), in both its wild type (WT) and mutant forms, at resolutions ranging from 1.30 to 2.25 Å. We show that the FdlA-NTD adopts a right-handed parallel β-helix fold, and possesses a substrate binding site composed of a long groove and a unique alkaline pocket. Our structural, biochemical, and enzymological analyses strongly suggest that FdlA-NTD utilizes catalytic residues different from other β-helix polysaccharide lyases, potentially representing a novel polysaccharide lyase family.
Sexual Inviolability of Minors in Central Kazakhstan: A Forensic Medical Analysis

Abstract Child sexual abuse is a social phenomenon that requires special attention from society, as it leads to the suffering of the most vulnerable part of the world's population. Child sexual abuse around the world not only persists, but also continues to grow, despite the active measures of the world community against it. Taking into account the high latency of crimes against sexual inviolability and sexual freedom of the person, measures for their detection and prevention are of particular relevance. All this requires constant monitoring of the current situation not only in the world or in the country, but also in each region of the country separately. Forensic medical examination of victims of sexual violence plays an important role in the analysis of the situation on this problem, not only in the legal, but also in the medical aspect. Research aim: Analysis of quantitative and qualitative indicators of crimes against the sexual inviolability of minors on the territory of Central Kazakhstan for the formation of indicators for assessing the current situation in the region and recommendations for the prevention of these types of crimes. Methods A retrospective analysis of the situation on the number and structure of sexual crimes committed against children from 2018 to 2021 was carried out, and their dynamics was studied. There were investigated 1252 forensic medical examinations on cases of sexual crimes against the sexual inviolability of the person on the territory of Central Kazakhstan. Methods of applied statistical analysis were used to analyz obtained results. Results A detailed analysis made it possible to assess the structure and peculiarities of sexual crimes committed against children on the territory of Central Kazakhstan over the specified period of time. The number of sexual crimes against minors in the region is increasing every year. Statistically significant age characteristics of victims and specific indicators characterizing the socio-psychological aspects of sexual crimes in the region were revealed. It has been established that in most cases the aggressor is part of the victim's family or is very close to it. The analysis of the age structure of children showed that boys from 6 to 14 years old and girls over 14 are more likely to become victims. In the studied territory, the number of sexual harassment of children is more often committed by one person, often known to them, and in most cases occurs in enclosed spaces than on the street. Conclusion The progressive growth of crimes against the sexual inviolability of children in the region requires strengthening measures to improve legal means of countering sexual crimes against minors. A detailed study of the issue made it possible to identify lacks (organizational, tactical, diagnostic) and give the forensic medical examination of sexual conditions a qualitatively new level. The strategy of combating sexual crimes against minors should be based on active preventive work. Key words: forensic medical examination, sexual violence, Central Kazakhstan, children, minors.
The Expression Levels and Concentrations of PD-1 and PD-L1 Proteins in Septic Patients: A Systematic Review

Sepsis is a series of life-threatening organ dysfunction caused by an impaired host response to infection. A large number of molecular studies of sepsis have revealed complex interactions between infectious agents and hosts that result in heterogeneous manifestations of sepsis. Sepsis can cause immunosuppression and increase the expression of checkpoint inhibitor molecules, including programmed death protein (PD-1) and programmed death ligand 1 (PD-L1), and thus PD-1 and PD-L1 are thought to be useful as diagnostic and prognostic tools for sepsis. PD-1 is an inhibitor of both adaptive and innate immune responses, and is expressed on activated T lymphocytes, natural killer (NK) cells, B lymphocytes, macrophages, dendritic cells (DCs), and monocytes, whereas PD-L1 is expressed on macrophages, some activated T and B cells, and mesenchymal stem cells as well as various non-hematopoietic cells. This systematic review aims to assess the PD-1 and PD-L1 protein expression levels and concentrations in septic and other infectious patients.
Microbial profile of lacrimal system Dacryoliths in American Midwest patient population

PURPOSE: Dacryoliths of the canalicular pathway are classically attributed to Actinomyces species as the most common organism. However, global shifts toward Streptococcus and Staphylococcus species have been reported. The objective of this article is to update the American Midwest epidemiology of lacrimal system dacryoliths for targeted clinical treatment. MATERIALS AND METHODS: A retrospective chart review from January 2015 to 2021 of patients with a history of surgical procedure for lacrimal removal of dacryolith for canaliculitis, canalicular obstruction, dacryocystitis, and nasolacrimal duct obstruction was included. Specimens were sent for histopathological evaluation and microbial culture. RESULTS: A total of 48 specimens were included. The most common organism isolated for canalicular pathology was Actinomyces spp (23%), followed by Staphylococcus spp (21%) and Streptococcus spp (19%). Histopathological staining accounted for 45% of Actinomyces isolation when culture data inconclusive. In a subgroup analysis of lacrimal sac dacryoliths, the most common organism was Staphylococcus spp (29%). Actinomyces species were not isolated from the lacrimal sac or nasolacrimal duct. CONCLUSION: Actinomyces maintains a microbial predominance in canalicular dacryoliths and requires careful culture and histopathological analysis for its fastidious nature. Lacrimal sac and nasolacrimal duct dacryolith found no isolates of Actinomyces, and the most common organism was Staphylococcus.
The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study

The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera and species belonging to the ESKAPEE group, 400 ARGs associated with this microbial group, and three classes of integrase genes were determined. A taxonomic analysis revealed that Acinetobacter was the dominant bacterial genus, whereas Acinetobacter baumannii and Escherichia coli were the dominant bacterial species. The analyzed samples were characterized by the highest concentrations of the following ARGs: blaGES, blaOXA-58, blaTEM, qnrB, and qnrS. Acinetobacter baumannii, E. coli, and genes encoding resistance to β-lactams (blaVEB-1, blaIMP-1, blaGES, blaOXA-58, blaCTX-M, and blaTEM) and fluoroquinolones (qnrS) were detected in samples of river water collected downstream from the wastewater discharge point. The correlation analysis revealed a strong relationship between A. baumannii (bacterial species regarded as an emerging human pathogen) and genes encoding resistance to all tested groups of antimicrobials. The transmission of the studied bacteria (in particular A. baumannii) and ARGs to the aquatic environment poses a public health risk.
The Impact of Public Service Motivation on Job Satisfaction in Public Sector Employees: The Mediating Roles of Work Engagement and Organizational Commitment

In the public service sector, staff’s motivation is of great significance for the developments of both the service team and the governmental service departments. This research develops a model to study the effects of public service motivation on job satisfaction and further assesses the mediation effects of work engagement and organizational commitment. Five-hundred and fifty employees from Chinese public service sectors were engaged in the questionnaire-based study. The results show that public service motivation plays important roles in work engagement, organizational commitment, and job satisfaction. Furthermore, work engagement and organizational commitment are the factors to mediate public service motivation and job satisfaction. Therefore, our findings suggest that staff in the public sector with enhanced public service motivation generally appear more involved in their work, are more loyal to their organizations, and demonstrate more positive work attitudes to improve productivity.
Evidence for Light and Tissue Specific Regulation of Genes Involved in Fructan Metabolism in Agave tequilana

Plant Glycoside Hydrolase Family 32 (PGHF32) contains the fructosyltransferases and fructan exohydrolase enzymes responsible for fructan metabolism, in addition to closely related vacuolar and cell wall acid invertases. Agave species produce complex and dynamic fructan molecules (agavins) requiring 4 different fructosyltransferase activities (1-SST, 1-FFT, 6G-FFT and 6-SFT) for their synthesis. Combined analysis of RNAseq and genome data for A. tequilana led to the characterization of the genes encoding 3 fructosyltransferases for this species and support the hypothesis that no separate 6-SFT type enzyme exists in A. tequilana, suggesting that at least one of the fructosyltransferases identified may have multiple enzymatic activities. Structures for PGHF32 genes varied for A. tequilana and between other plant species but were conserved for different enzyme types within a species. The observed patterns are consistent with the formation of distinct gene structures by intron loss. Promoter analysis of the PGHF32 genes identified abundant putative regulatory motifs for light regulation and tissue-specific expression, and these regulatory mechanisms were confirmed experimentally for leaf tissue. Motifs for phytohormone response, carbohydrate metabolism and dehydration responses were also uncovered. Based on the regulatory motifs, full-length cDNAs for MYB, GATA, DOF and GBF transcription factors were identified and their phylogenetic distribution determined by comparison with other plant species. In silico expression analysis for the selected transcription factors revealed both tissue-specific and developmental patterns of expression, allowing candidates to be identified for detailed analysis of the regulation of fructan metabolism in A. tequilana at the molecular level.
Maximizing Performance with Safe Meat Production of Broiler by Feeding Enzyme Supplemented Antibiotic Free Diet

The present work aimed at studying growth performance, carcass traits, and health status in broiler chicken-fed enzyme over a period of 4 weeks. A total of 300 day-old Lohmann broilers were randomly assigned to five treatment groups, each with 3 replicates (20). T1, T2, and T3 were provided as 0.05 %, 0.1 %, and 0.15 % of the enzyme. Whereas T4 and T5 were antibiotic and control, respectively. The results revealed no significant (P<0.05) difference in feed intake (T4 - 2182.50bg and T2 - 2227.00 abg) and live weight (T5 - 1897.50a g and T1 - 1790.50b). The highest survivability percent was found in the enzyme supplemented group (T1, T2 &T3- 100 %). Highest hemoglobin (T2 - 9.0 gm/dl), RBC (T2-3.9mill/cum), WBC (T1-14475 mill/cum), lymphocytes (T1 - 38.50 %), Monocytes (T1 - 2.00 %) PCV (39.96 %), MCV (T1 - 88.58), MCH (T2 - 30.85 Pg) and MCHC (T2 - 32.80) were found highest in the enzyme-treated groups, which is an indication of good health. E. coli and salmonella spp. The count was significantly (P<0.05) lower in birds fed a 0.15 % enzyme supplemented diet and with a descending order of 0.1 % and 0.5 % enzyme level. Salmonella sp. and E Coli count was also significantly (p<0.05) higher in birds fed control and antibiotic. The results of the study demonstrate the beneficial effects of supplementing enzymes on body weight gain and dressed yield in the treated groups in broiler chicken. An enzyme is, therefore, suggested to be used as an alternative to antibiotics on broiler chicken rations for higher profitability.
Vues: practical mobile volumetric video streaming through multiview transcoding

The emerging volumetric videos offer a fully immersive, six degrees of freedom (6DoF) viewing experience, at the cost of extremely high bandwidth demand. In this paper, we design, implement, and evaluate Vues, an edge-assisted transcoding system that delivers high-quality volumetric videos with low bandwidth requirement, low decoding overhead, and high quality of experience (QoE) on mobile devices. Through an IRB-approved user study, we build a first-of-its-kind QoE model to quantify the impact of various factors introduced by transcoding volumetric content into 2D videos. Motivated by the key observations from this user study, Vues employs a novel multiview approach with the overarching goal of boosting QoE. The Vues edge server adaptively transcodes a volumetric video frame into multiple 2D views with the help of a few lightweight machine learning models and strategically balances the extra bandwidth consumption of additional views and the improved QoE, indicated by our QoE model. The client selects the view that optimizes the QoE among the delivered candidates for display. Comprehensive evaluations using a prototype implementation indicate that Vues dramatically outperforms existing approaches. On average, it improves the QoE by 35% (up to 85%), compared to single-view transcoding schemes, and reduces the bandwidth consumption by 95%, compared to the state-of-the-art that directly streams volumetric videos.
iFlood: A Stable and Effective Regularizer

Various regularization methods have been designed to prevent overfitting of machine learning models. Among them, a surprisingly simple yet effective one, called Flooding, is proposed recently, which directly constrains the training loss on average to stay at a given level. However, our further studies uncover that the design of the loss function of Flooding can lead to a discrepancy between its objective and implementation, and cause the instability issue. To resolve these issues, in this paper, we propose a new regularizer, called individual Flood (denoted as iFlood). With instance-level constraints on training loss, iFlood encourages the trained models to better fit the under-fitted instances while suppressing the confidence on over-fitted ones. We theoretically show that the design of iFlood can be intrinsically connected with removing the noise or bias in training data, which makes it suitable for a variety of applications to improve the generalization performances of learned models. We also theoretically link iFlood to some other regularizers by comparing the inductive biases they introduce. Our experimental results on both image classification and language understanding tasks confirm that models learned with iFlood can stably converge to solutions with better generalization ability, and behave consistently at instance-level.
Structure of stationary stable processes

A connection between structural studies of stationary non-Gaussian stable processes and the ergodic theory of nonsingular flows is established and exploited. Using this connection, a unique decomposition of a stationary stable process into three independent stationary parts is obtained. It is shown that the dissipative part of a flow generates a mixed moving average part of a stationary stable process, while the identity part of a flow essentially gives the harmonizable part. The third part of a stationary process is determined by a conservative flow without fixed points and by a related cocycle.
GAN-Based Super-Resolution And Segmentation Of Retinal Layers In Optical Coherence Tomography Scans

Optical Coherence Tomography (OCT) has been identified as a noninvasive and cost-effective imaging modality for identifying potential biomarkers for Alzheimer’s diagnosis and progress detection. Current hypotheses indicate that retinal layer thickness, which can be assessed via OCT scans, is an efficient biomarker for identifying Alzheimer’s disease. Due to factors such as speckle noise, a small target region, and unfavorable imaging conditions manual segmentation of retina layers is a challenging task. Therefore, as a reasonable first step, this study focuses on automatically segmenting retinal layers to separate them for subsequent investigations. Another important challenge commonly faced is the lack of clarity of the layer boundaries in retina OCT scans, which compels the research of super-resolving the images for improved clarity. learning progress for the segmentation tasks. Generative adversarial networks (GANs) are a prominent field of deep learning which achieved astonishing performance in semantic segmentation. Conditional adversarial networks as a general-purpose solution to image-to-image translation problems not only learn the mapping from the input image to the output image but also learn a loss function to train this mapping. We propose a GAN-based segmentation model and evaluate incorporating popular networks, namely, U-Net and ResNet, in the GAN architecture with additional blocks of transposed convolution and sub-pixel convolution for the task of upscaling OCT images from low to high resolution by a factor of four. We also incorporate the Dice loss as an additional reconstruction loss term to improve the performance of this joint optimization task. Our best model configuration empirically achieved the Dice coefficient of 0.867 and mIOU of 0.765.
Compound flood events: analysing the joint occurrence of extreme river discharge events and storm surges in northern and central Europe

Abstract. The simultaneous occurrence of extreme events gained more and more attention from scientific research in the last couple of years. Compared to the occurrence of single extreme events, co-occurring or compound extremes may substantially increase risks. To adequately address such risks, improving our understanding of compound flood events in Europe is necessary and requires reliable estimates of their probability of occurrence together with potential future changes. In this study compound flood events in northern and central Europe were studied using a Monte Carlo-based approach that avoids the use of copulas. Second, we investigate if the number of observed compound extreme events is within the expected range of 2 standard deviations of randomly occurring compound events. This includes variations of several parameters to test the stability of the identified patterns. Finally, we analyse if the observed compound extreme events had a common large-scale meteorological driver. The results of our investigation show that rivers along the west-facing coasts of Europe experienced a higher amount of compound flood events than expected by pure chance. In these regions, the vast majority of the observed compound flood events seem to be related to the cyclonic westerly general weather pattern (Großwetterlage).
Comparison of Outcomes between Primary Repair and Ileostomy in Children with Typhoid Perforation

Aim: To compare the outcomes in term of complications and mortality between primary repair and ileostomy in children presented with typhoid perforation. Study design: Randomized comparative/observational. Place and duration of study: Sohail Medical Centre Timergara from 1 st January 2019 to 31 st December 2019. Methods: Seventy four patients of both genders with typhoid perforation were included in this study. Patients ages were up to 12 years. All the patients were categorized into two equal groups. Each group contains 37 patients. Group I received primary repair and group II consist received ileostomy. Postoperative outcomes such as complications and mortality were comparing between both groups. Results: 48 (64.86%)were males and 26 (35.14%) were females with mean age 8.76+2.58 years. Group II had significantly higher complications 20 (54.05%) as compared to group I 8 (21.62%) with p-value <0.05. Mortality rate was high in Group 1 13.51%as compared to Group II 8.11%. Conclusion: Primary repair is safe and effective in term of postoperative complications and mortality as compared to ileostomy in patients with typhoid perforation.
PPA_A_374445 2149..2158

Purpose: To evaluate the psychometric properties and feasibility of the Thai version of Compliance-Questionnaire-Rheumatology (CQR)-19. Patients and Methods: A cross-sectional study was conducted in the Rheumatology clinic of Siriraj hospital from October 2020 to March 2022. Literate patients diagnosed with rheumatoid arthritis and aged ≥ 18 years old were included. Participants completed the Thai CQR-19 at baseline and during a follow-up visit. A pill count at the follow-up visit indicating that ≤ 80% of prescribed pills were taken was defined as poor compliance. Feasibility was assessed by the time to completion and the number of missing questions. Results: 156 participants were enrolled and 88% were in good compliance. The sensitivity of the CQR-19 to detect good compliance was 78.2% and the specificity was 27.8%. The Thai CQR-19 showed moderate agreement (Gwet’s AC1= 0.61, p < 0.01) with 72.2% agreement against pill counts. The Thai CQR-19 had good internal consistency (Cronbach’s alpha = 0.83) with moderate test-retest reliability (intraclass coefficients = 0.64, 95% CI 0.54–0.73; p < 0.05). There was a weak correlation between the Thai CQR-19 and disease activity (rs = 0.20, p < 0.05), and no correlation with functional disability (rs = 0.09, p = 0.25). The median (IQR) completion time was 4.42 (3.25–6.16) minutes and 114 (73%) completed all 19 questions. Conclusion: The Thai version of CQR-19 is a valid and reliable tool to assess medication compliance in Thai patients with RA. To ensure completeness and accuracy, this questionnaire may be administered by interview.