Miracle-dz/UniFAR-FTUs huggingface dataset:
Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice

Mucin 5AC (MUC5AC) is excessively secreted in the respiratory tract of patients with asthma. Suppressing this secretion is important for improving the air passages, which facilitates easy breathing. We have previously reported that the addition of type IV collagen, a typical extracellular matrix (ECM) protein, to the culture medium for human cell lines and primary cells reduced MUC5AC secretion. In this report, we further investigated the effect of type IV collagen on MUC5AC secretion in vivo. We employed ovalbumin (OVA)-sensitized mice to model of asthma and exposed them to type IV collagen to verify the reducing effect of MUC5AC in vivo. The amount of MUC5AC in bronchoalveolar lavage fluid was examined after nebulization of type IV collagen. Hypersecretion of MUC5AC of the OVA-sensitized mice was suppressed by type IV collagen exposure in a time- and dose-dependent manner. Furthermore, type IV collagen exposure to OVA-sensitized mice decreased integrin α2 and β1 expression in the lungs and increased the levels of Akt and extracellular signal-regulated kinase (ERK) phosphorylation in the trachea. These results suggest that type IV collagen suppresses MUC5AC hypersecretion via modulating integrin expression and Akt/ERK phosphorylation in the respiratory tract of the OVA-sensitized mice.
Long-term gamma transcranial alternating current stimulation improves the memory function of mice with Alzheimer’s disease

Background The main manifestation of Alzheimer’s disease (AD) in patients and animal models is impaired memory function, characterized by amyloid-beta (Aβ) deposition and impairment of gamma oscillations that play an important role in perception and cognitive function. The therapeutic effect of gamma band stimulation in AD mouse models has been reported recently. Transcranial alternating current stimulation (tACS) is an emerging non-invasive intervention method, but at present, researchers have not completely understood the intervention effect of tACS. Thus, the intervention mechanism of tACS has not been fully elucidated, and the course of treatment in clinical selection also lacks theoretical support. Based on this issue, we investigated the effect of gamma frequency (40 Hz) tACS at different durations in a mouse model of AD. Materials and methods We placed stimulating electrodes on the skull surface of APP/PS1 and wild-type control mice (n = 30 and n = 5, respectively). Among them, 20 APP/PS1 mice were divided into 4 groups to receive 20 min 40 Hz tACS every day for 1–4 weeks. The other 10 APP/PS1 mice were equally divided into two groups to receive sham treatment and no treatment. No intervention was performed in the wild-type control mice. The short-term memory function of the mice was examined by the Y maze. Aβ levels and microglia in the hippocampus were measured by immunofluorescence. Spontaneous electroencephalogram gamma power was calculated by the average period method, and brain connectivity was examined by cross-frequency coupling. Results We found that the long-term treatment groups (21 and 28 days) had decreased hippocampal Aβ levels, increased electroencephalogram spontaneous gamma power, and ultimately improved short-term memory function. The treatment effect of the short-term treatment group (7 days) was not significant. Moreover, the treatment effect of the 14-day treatment group was weaker than that of the 21-day treatment group. Conclusion These results suggest that long-term gamma-frequency tACS is more effective in treating AD by reducing Aβ load and improving gamma oscillation than short-term gamma-frequency tACS.
Tectonics of kinetic architecture: Moving envelope, changing space and the shades of the shed

Buildings in our day are no more frozen pieces of music as ascribed by Johann Wolfgang von Goethe. They can move, rotate, flip and perform various physical gestures. This paper aims to explore the spatial, aesthetical, and technical characteristics of kinetic buildings within the framework of the theory of tectonics, which is still far away from covering the art form of movement. The study has been organized into two phases. The first phase sets up the theoretical framework of the topic by exploring the main dichotomy of the tectonic discourse on “ontological and representational parts of the building” and introduces the main concepts of kinetic architecture related to the characterization of architectural space. The second phase is a case study on The Shed as also known as Culture Shed in Hudson Yards in New York City. This part focuses on the interrelations between tectonics and the physical movement of architectural elements. The results indicate that the type of movement and the role of moving elements change not only the architectural space but also the tectonic character of the building. While some movements and moving parts are directly related to the representational aspects, some change the ontological character of the building. The impact of the movement on the tectonic character is a topic that hasn't been well investigated yet and has the potential to be developed through further research. As a result, the theoretical findings of this study can contribute to tectonic thinking during the design process of kinetic architectural products.
Daily and weekly external loads in the microcycle: Characterization and comparison between playing positions on amateur soccer

Ensuring adequate levels of training and recovery to maximize player performance is critical; however, there are methodological challenges in designing a periodized training program for soccer teams. This study aims to describe and characterize the daily and weekly external load in an amateur soccer team and based on the weighting factors determined by the match reference, compare the external loads between playing positions. Twenty-four amateur soccer players (22.3 ± 1.7 years) were monitored using a global positioning system. Data collected comprises 19 competitive microcycles with a standard structure composed of 3 training sessions (matchday-5, matchday-3, and matchday-2) and one match. Match-reference values were calculated as the mean of the five best values recorded during official matches. The results show, on matchday-5 session, the existence of significant differences between playing positions to relative total distance covered (p = 0.050), relative sprint distance (p = 0.001), relative moderate-intensity accelerations (p < 0.001), relative high-intensity accelerations (p = 0.003), relative moderate-intensity decelerations (p < 0.001), and relative high-intensity decelerations (p = 0.017). On matchday-3 session, there are significant differences to relative very high-speed running distance (p = 0.017) and relative moderate-intensity decelerations (p = 0.014). On matchday-2 session, there are significant differences to relative high-speed running distance (p = 0.025), relative very high-speed running distance (p = 0.008), and relative moderate-intensity decelerations (p < 0.001). Weekly significant differences are observed between the playing positions to relative moderate-intensity accelerations (p = 0.002), relative high-intensity accelerations (p < 0.001), and relative moderate-intensity decelerations (p < 0.001). The weekly load is characterized by a greater weighting on accelerations and decelerations, compared to distances at very-high speed and sprint. The training loads must respect a standard training model that contemplates the individualization of the physical demands of the match, for each playing position, as for each individual.
Sci-Seq of Human Fetal Salivary Tissue Introduces Human Transcriptional Paradigms and a Novel Cell Population

Multiple pathologies and non-pathological factors can disrupt the function of the non-regenerative human salivary gland including cancer and cancer therapeutics, autoimmune diseases, infections, pharmaceutical side effects, and traumatic injury. Despite the wide range of pathologies, no therapeutic or regenerative approaches exist to address salivary gland loss, likely due to significant gaps in our understanding of salivary gland development. Moreover, identifying the tissue of origin when diagnosing salivary carcinomas requires an understanding of human fetal development. Using computational tools, we identify developmental branchpoints, a novel stem cell-like population, and key signaling pathways in the human developing salivary glands by analyzing our human fetal single-cell sequencing data. Trajectory and transcriptional analysis suggest that the earliest progenitors yield excretory duct and myoepithelial cells and a transitional population that will yield later ductal cell types. Importantly, this single-cell analysis revealed a previously undescribed population of stem cell-like cells that are derived from SD and expresses high levels of genes associated with stem cell-like function. We have observed these rare cells, not in a single niche location but dispersed within the developing duct at later developmental stages. Our studies introduce new human-specific developmental paradigms for the salivary gland and lay the groundwork for the development of translational human therapeutics.
Analysis of the 16S rRNA gene for the characterization of the bacterial community of the Lambro river (Italy)

Characterization of the microbial community of a river can provide various indications, such as its general state of health or the presence of contamination. Furthermore, the study of Bacteroidetes, which have a high degree of host specificity, can provide information on the species involved in any fecal contamination. The analysis of the 16S rRNA was used to characterize the bacterial community of the Lambro river (Italy) through. The results, which were obtained by analyzing water from 15 sampling points, show a reduction in the complexity of the bacterial community as the river enters a densely populated region. The cause could be a source of chemical or physical contamination that carries out a positive selection toward some bacterial species and negative toward others. In addition, a notable increase in the percentage of Bacteroidetes was reported, especially when the river enters regions characterized by high agricultural and livestock activity, such as cattle and pig farming. However, in the samples taken from this area, no Bacteroidetes ascribable to these two species or to the other species considered (i.e., human, dog, and cat) were found. Surprisingly, suspected bacterial contamination of swine origin was identified in a sparsely populated region characterized by small family farms. Finally, the efficient treatment of urban wastewater was confirmed as no markers of fecal pollution of human origin were identified.
Traditional Chinese medicine prescriptions (XJZ, JSS) ameliorate spleen inflammatory response and antioxidant capacity by synergistically regulating NF-κB and Nrf2 signaling pathways in piglets

Weaning transition generally impairs the immune system, inducing immune disturbance, which may be associated with post-weaning diarrhea and high mortality in piglets. The spleen is a pivotal lymphatic organ that plays a key role in the establishment of the immune system. Traditional Chinese medicine (TCM) prescriptions, XiaoJianZhong (XJZ) and Jiansananli-sepsis (JSS), are widely used prescriptions for treating spleen damage and diarrhea. Here, we hypothesized that XJZ and JSS maintain the spleen physiological function by ameliorating antioxidant capacity and inflammatory response in weaned piglets. In this study, 18 weaned piglets were assigned to the Control, XJZ and JSS groups. By hematoxylin and eosin staining, hematological analysis, flow cytometric analysis, qRT-PCR and western blot, the effects of both TCM prescriptions on the spleen antioxidant defense system and inflammatory pathway were explored. Results showed that both TCM treatment significantly ameliorated the weaning-induced morphological damage in piglets, as evidenced by clearer and more perfect spleen histology, as well as higher relative area of white pulp. Meanwhile, both XJZ and JSS exerted better blood parameters, as supported by the changes of monocyte level and lymphocyte subpopulations CD4+/CD8+ ratio. Furthermore, the levels of inflammatory markers, IL1β, IL6, IL8, and TNF-α in the spleen were markedly decreased after supplemented with both TCM prescriptions. Importantly, the inhibition of nuclear factor-kappaB (NF-κB) and its downstream effector genes (IL6, IL8, and TNF-α) in both XJZ and JSS treatment groups further confirmed alleviation of inflammatory responses in the spleen. In addition, both XJZ and JSS enhanced the antioxidant capacity of the spleen by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)-activated antioxidant defense system. Notably, the results of PCA and network correlation analysis indicated that XJZ and JSS treatment altered the expression profiles of inflammatory and antioxidant-related factors in the spleen of weaned-piglets, which may involve the synergy of NF-κB and Nrf2 signaling pathways. In summary, our study showed that TCM prescriptions, XJZ and JSS could ameliorate inflammatory response and antioxidant capacity in the spleen by synergistically regulating NF-κB and Nrf2 signaling pathways in piglets.
The balbyter ant Camponotus fulvopilosus combines several navigational strategies to support homing when foraging in the close vicinity of its nest

Many insects rely on path integration to define direct routes back to their nests. When shuttling hundreds of meters back and forth between a profitable foraging site and a nest, navigational errors accumulate unavoidably in this compass- and odometer-based system. In familiar terrain, terrestrial landmarks can be used to compensate for these errors and safely guide the insect back to its nest with pin-point precision. In this study, we investigated the homing strategies employed by Camponotus fulvopilosus ants when repeatedly foraging no more than 1.25 m away from their nest. Our results reveal that the return journeys of the ants, even when setting out from a feeder from which the ants could easily get home using landmark information alone, are initially guided by path integration. After a short run in the direction given by the home vector, the ants then switched strategies and started to steer according to the landmarks surrounding their nest. We conclude that even when foraging in the close vicinity of its nest, an ant still benefits from its path-integrated vector to direct the start of its return journey.
Inequalities in access to community-based diabetes examination and its impact on healthcare utilization among middle-aged and older adults with diabetes in China

Globally, diabetes and its complications are becoming one of the leading challenges in health governance. As health inequalities and primary care services related to diabetes are gaining traction, the status of community-based diabetes examination largely remains unclear in the literature. This study aims to investigate inequalities in access to community-based diabetes examination among people with diabetes and to analyze its impact on healthcare utilization. Data from the 2018 China Health and Retirement Longitudinal Study (CHARLS) were applied, and a total of 767 patients with diabetes were included. Inequalities in community-based diabetes examination were illustrated by the concentration curve and normalized concentration index. Propensity score matching (PSM) were used to identify the impact of community-based diabetes examination on outpatient and inpatient care utilization. We found that community-based diabetes examination was accessible to 23.08% of the respondents, of which 76.84% were free, and the highest frequency was 2–6 times per year, accounting for 47.46%. Community-based diabetes examinations were more concentrated among people with poorer-economic condition (95% confidence interval, 95%CI = −0.104, p = 0.0035), lower-education level (95%CI = −0.092, p = 0.0129), and less-developed areas (95%CI = −0.103, p = 0.0007). PSM analyses showed that community-based diabetes examination increased the utilization of outpatient care (odds ratio, OR = 1.989, 95%CI = 1.156–3.974) and decreased the use of inpatient care (OR = 0.544, 95%CI = 0.325–0.909), and the sensitivity analyses confirmed the robustness of the results. This study is the first to examine the status and inequalities of community-based regular diabetes examination and its effect on the likelihood of healthcare utilization among patients with diabetes. The findings suggest that the overall level of community-based diabetes examination is low, and there are pro-socioeconomically disadvantaged inequalities. The value of community-based diabetes examination should be recognized to help person with diabetes face up to their health needs for better disease control and health promotion.
Molecular Composition of Dissolved Organic Matter in the Changjiang (Yangtze River) – Imprints of Anthropogenic Impact

Understanding the biogeochemical transformation of dissolved organic matter (DOM) across fluvial networks will ultimately help to predict anthropogenic influences. To date, few studies have evaluated the anthropogenic impact on the spatial and temporal changes of DOM composition in large river systems. Here, FT-ICR-MS combined with excitation-emission matrix spectroscopy (EEMs) and biomarkers were applied to resolve chemical differences of DOM collected from the Changjiang basin at different hydrological and environmental conditions. PCA and cluster analysis illustrated that samples collected from lake systems and northern and southern tributaries differed from the two batches of main stream samples, particularly due to higher contribution of nitrogen and sulfur containing compounds. Correlation of land-use information along the tributaries with different PCA loadings indicated that agricultural, forest and wetland areas and wastewater discharge control the composition of DOM within these subregions. Higher heteroatom content (especially CHONx) in the low discharge period (2009) may be contributed by paddy soil leaching into groundwater. The relative peak magnitude of sulfur containing formulas was elevated during flood season (2010), which may be related to pollutions in areas of high population density. In addition, lignin phenol concentrations were higher in the flood season because of elevated soil erosion. Consequently, land use and human activities can strongly alter the quality and composition of DOM in watersheds flowing through densely populated regions, which may also impact or influence the riverine carbon flux in anthropogenically disturbed river systems.
Y-specific amh allele, amhy, is the master sex-determining gene in Japanese flounder Paralichthys olivaceus

Japanese flounder (Paralichthys olivaceus) is an important marine fish species of both fisheries and aquaculture in Northeast Asia. The commercial interest for all-female progenies due to several sex-related traits has prompted basic research on the mechanisms of sex determination in this species. By conducting a linkage analysis of the sex-determining locus, we initially identified 12 microsatellite markers linked to sex in 11 scaffolds, whose localization was restricted to a specific region of linkage group 9. Sequence analysis of this region identified 181 genes based on the UniProt database annotations. Among them, the amh gene was considered a potential candidate for sex determination because this gene is known to have taken over the role of sex determination in many teleosts. An in-depth sequence analysis of both the coding and non-coding regions of amh in XX and XY individuals detected nine SNPs linked with maleness. However, because these substitutions were synonymous, the upstream and downstream regions of amh were also investigated and a male-specific variant with deletions in the promoter region was detected. This truncated Y-specific amh variant was named amhy, and the amh shared by both sexes was named amhx. The association analysis using both females and males of the genotypic sex inferred by the presence/absence of amhy found complete association with phenotypic sex and genotype. Gene expression analysis in larvae derived from a single-pair progeny by quantitative real-time PCR detected amhy transcripts in the larval trunks between 20 and 100 days after hatching only in XY larvae. Localization of amhy by in situ hybridization was detected in presumptive Sertoli cells of XY gonads. Expression of amhx was almost undetectable in both XX and XY genotypes. Loss of Amh function by CRISPR-Cas9 induced male-to-female sex reversal, indicating that this gene was necessary for the masculinization of XY individuals. In conclusion, the complete linkage of amhy with males, its early expression in XY gonads before testicular differentiation, and the induction of sex reversal by loss-of-function mutation support the view that amhy is the sex-determining gene in this species.
Hyperfocus or flow? Attentional strengths in autism spectrum disorder

The comorbidity of autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) diagnoses is well established. An ASD diagnosis is associated with elevated ADHD traits and symptoms, as well as strengths in attention. In the ASD literature, attentional strengths have been described as maladaptive (e.g., hyperfocus), in contrast with positive portrayals in the typically developing population (e.g., flow). The objective of this study was to (1) compare profiles of attentional strengths and weaknesses in ASD and ADHD and (2) determine whether attentional strengths in ASD are associated with impairment, poorer cognitive flexibility, and perseveration/perfectionism. In a community sample of 5,744 children and youth, 131 children were reported as having a diagnosis of ASD (mean age 10.3 years) and 346 children were reported as having a diagnosis of ADHD (mean age 10.7 years). We used the Strengths and Weaknesses of Attention-Deficit/Hyperactivity-symptoms and Normal-behaviors (SWAN) rating scale to calculate attentional and hyperactive/impulse control strength and weakness counts and scores. The Autism-Spectrum Quotient Switching factor served as a measure of cognitive flexibility. Impairment was assessed with the Columbia Impairment Scale. We used the symmetry/ordering factor on the Toronto Obsessive-Compulsive Scale as a measure of perseveration/perfectionism. No differences were found between the ADHD and ASD groups in SWAN weakness scores, symptoms, or hyperactive/impulse control strengths; however, autistic children had higher rates of attentional strengths [odds ratio: 5.7, 95% CI (2.8, 11.6), p < 0.0001]. Post-hoc pairwise testing identified four attentional strengths with significantly higher rates in ASD than in ADHD. Attentional strength scores were not associated with impairment or poor cognitive flexibility, but predicted levels of perseveration/perfectionism. The effect of attentional strengths on impairment and cognitive flexibility did not differ between autistic and Control children, but the higher perseveration/perfectionism scores seen in ASD were not found in Control children. ASD is associated with a pattern of attentional strengths that is not found in ADHD Characterizing the full range of attentional abilities in autistic children may explain variability in outcomes such as quality-of-life indicators and identify protective factors, providing targets for strength-based behavioral interventions. The clinical and etiological implications of the subgroup of autistic children with attentional strengths require further investigation.