Miracle-dz/UniFAR-FTUs huggingface dataset:
Astrocytes-Derived Small Extracellular Vesicles Hinder Glioma Growth

All cells are capable of secreting extracellular vesicles (EVs), which are not a means to eliminate unneeded cellular compounds but represent a process to exchange material (nucleic acids, lipids and proteins) between different cells. This also happens in the brain, where EVs permit the crosstalk between neuronal and non-neuronal cells, functional to homeostatic processes or cellular responses to pathological stimuli. In brain tumors, EVs are responsible for the bidirectional crosstalk between glioblastoma cells and healthy cells, and among them, astrocytes, that assume a pro-tumoral or antitumoral role depending on the stage of the tumor progression. In this work, we show that astrocyte-derived small EVs (sEVs) exert a defensive mechanism against tumor cell growth and invasion. The effect is mediated by astrocyte-derived EVs (ADEVs) through the transfer to tumor cells of factors that hinder glioma growth. We identified one of these factors, enriched in ADEVs, that is miR124. It reduced both the expression and function of the volume-regulated anion channel (VRAC), that, in turn, decreased the cell migration and invasion of murine glioma GL261 cells.
Antibodies to Kv1 potassium channel-complex proteins leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 in limbic encephalitis, Morvan’s syndrome and acquired neuromyotonia

Antibodies that immunoprecipitate 125I-α-dendrotoxin-labelled voltage-gated potassium channels extracted from mammalian brain tissue have been identified in patients with neuromyotonia, Morvan’s syndrome, limbic encephalitis and a few cases of adult-onset epilepsy. These conditions often improve following immunomodulatory therapies. However, the proportions of the different syndromes, the numbers with associated tumours and the relationships with potassium channel subunit antibody specificities have been unclear. We documented the clinical phenotype and tumour associations in 96 potassium channel antibody positive patients (titres >400 pM). Five had thymomas and one had an endometrial adenocarcinoma. To define the antibody specificities, we looked for binding of serum antibodies and their effects on potassium channel currents using human embryonic kidney cells expressing the potassium channel subunits. Surprisingly, only three of the patients had antibodies directed against the potassium channel subunits. By contrast, we found antibodies to three proteins that are complexed with 125I-α-dendrotoxin-labelled potassium channels in brain extracts: (i) contactin-associated protein-2 that is localized at the juxtaparanodes in myelinated axons; (ii) leucine-rich, glioma inactivated 1 protein that is most strongly expressed in the hippocampus; and (iii) Tag-1/contactin-2 that associates with contactin-associated protein-2. Antibodies to Kv1 subunits were found in three sera, to contactin-associated protein-2 in 19 sera, to leucine-rich, glioma inactivated 1 protein in 55 sera and to contactin-2 in five sera, four of which were also positive for the other antibodies. The remaining 18 sera were negative for potassium channel subunits and associated proteins by the methods employed. Of the 19 patients with contactin-associated protein-antibody-2, 10 had neuromyotonia or Morvan’s syndrome, compared with only 3 of the 55 leucine-rich, glioma inactivated 1 protein-antibody positive patients (P < 0.0001), who predominantly had limbic encephalitis. The responses to immunomodulatory therapies, defined by changes in modified Rankin scores, were good except in the patients with tumours, who all had contactin-associated-2 protein antibodies. This study confirms that the majority of patients with high potassium channel antibodies have limbic encephalitis without tumours. The identification of leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 as the major targets of potassium channel antibodies, and their associations with different clinical features, begins to explain the diversity of these syndromes; furthermore, detection of contactin-associated protein-2 antibodies should help identify the risk of an underlying tumour and a poor prognosis in future patients.
Changes in psychological wellbeing, attitude and information-seeking behaviour among people at the epicentre of the COVID-19 pandemic: a panel survey of residents in Hubei province, China

Abstract While most research focuses on the clinical treatment of COVID-19, fewer studies have investigated individuals' responses towards this novel infectious disease. This study aims to report the temporal changes in individuals' psychological wellbeing, perceived discrimination, sociopolitical perceptions and information-seeking behaviours among the general public in Hubei, China. Data were obtained from a two-wave survey of 1902 respondents aged 18–80 in Hubei province during the peak and mitigation stages of the outbreak. The results showed that the prevalence of psychological distress dropped from over 75% to around 15% throughout the study period, but perceived discrimination remained stable. Female, middle-aged, well-educated respondents and those employed in government/public institutions/state-owned enterprises tended to report more distress. While respondents' attention on COVID-19 information kept high and stable, their sources of information diversified across different sociodemographic groups. Over time, people obtained more social support from neighbourhoods than from their friends and relatives or non-government organisations. Over 80% of respondents were satisfied with the performance of the central government, which was notably higher than their ratings on the local government and neighbourhood/village committees. The findings of this research are informative for formulating effective intervention strategies to tackle various psychosocial problems during COVID-19.
Flux variation in a Siberian taiga forest near Yakutsk estimated by a one‐dimensional model with routine data, 1986–2000

For a larch forest site near Yakutsk, energy and water fluxes are estimated and their variations are investigated using a one‐dimensional land surface model with daily routine data. The estimation period is 1986–2000, including the period before the start of tower observation (1997) on the left bank of the Lena River. The land surface model includes three submodels: vegetation, snow cover, and soil. The model can calculate water and energy fluxes above and within the forest if meteorological data over the forest are given as input. Data used in this study are Baseline Meteorological Data in Siberia (BMDS) Version 3. This set comprises daily data of main meteorological elements. The procedure is as follows: (1) preparation of equations between routine Yakutsk data and larch tower data; (2) estimation of past meteorological data over the taiga using those equations; and (3) estimation of fluxes using the one‐dimensional model. The date of leaf‐out start is parameterized with soil temperature and accumulated air temperature. It corresponds to the green‐up time obtained from satellite normalized difference vegetation index (NDVI) data. Estimated monthly deviation of net radiation, Rn, is 10 Wm−2. Its maximum is 20 Wm−2. The monthly deviation of latent heat flux, LE, is less than 12 Wm−2. Its warm season average is less than 7 Wm−2. Although the magnitude of LE is almost zero in December and January, and several Wm−2 aside from these 2 months, the sensible heat flux, H, sometimes becomes negative during the cold season. The variation of evapotranspiration is considerably smaller than that of precipitation. The evapotranspiration (E) normalized by potential evaporation (Ep), E/Ep is 0·37–0·44 in the warm season; it tends to be large when leaf‐out starts early. The amount of evapotranspiration in the warm season can be estimated from Ep within an error of 5 mm using this relationship. Copyright © 2007 John Wiley & Sons, Ltd.
Large freshwater plesiosaurian from the Cretaceous (Aptian) of Australia

Benson, R.B.J., Fitzgerald, E.M.G. , Rich, T.H. & Vickers-Rich, P., 2013. Large freshwater plesiosaurian from the Cretaceous (Aptian) of Australia. Alcheringa 37, 1–6. ISSN 0311-5518 We report a large plesiosaurian tooth from the freshwater early–middle Aptian (Early Cretaceous) Eumeralla Formation of Victoria, Australia. This, combined with records of smaller plesiosaurian teeth with an alternative morphology, provides evidence for a multitaxic freshwater plesiosaurian assemblage. Dental and body size differences suggest ecological partitioning of sympatric freshwater plesiosaurians analogous to that in modern freshwater odontocete cetaceans. The evolutionarily plastic body plan of Plesiosauria may have facilitated niche differentiation and helped them to exclude ichthyosaurs from freshwater environments during the Mesozoic. However, confirmation of this hypothesis requires the discovery of more complete remains. Roger B.J. Benson [roger.benson@earth.ox. ac.uk], Department of Earth Sciences, University of Oxford, South Parks Road, Oxford OX1 3AN, UK; Erich M.G. Fitzgerald [efitzgerald@museum.vic. gov.au], Thomas H. Rich [trich@museum.vic. gov.au], Museum Victoria, GPO Box 666, Melbourne, Victoria 3001, Australia; Thomas H. Rich and Patricia Vickers-Rich [pat.rich@monash.edu], School of Geosciences, Monash University, Clayton, Victoria 3800, Australia. Received 30.10. 2012; revised 27.1. 2013; accepted 31.1. 2013.
Nickel distribution and speciation in rapidly dehydroxylated goethite in oxide-type lateritic nickel ores: XAS and TEM spectroscopic (EELS and EFTEM) investigation

The distribution of Ni in four lateritic Ni-goethites that were rapidly dehydroxylated to form hematite by shock heating at 340/400°C and 800°C for 30 min was investigated using synchrotron X-ray diffraction (SXRD), TEM spectroscopy (EELS and EFTEM) and synchrotron X-ray absorption spectroscopy (XAS). The Ni K-edge EXAFS results for non-heated samples showed three distinct Ni–Fe shells, including two edge-sharing (R Ni–Fe ∼ 3.01 and R Ni–Fe ∼ 3.22 Å) and a double corner-sharing (R Ni–Fe ∼ 3.52 Å) complex for most of the samples. These interatomic distances are indicative of Ni substituting for Fe in goethite, which has resulted in an expansion in the goethite structure along the a-axis direction and a contraction along the b-axis direction. Ravensthorpe Ni goethite was considerably different from the other goethites, with two Ni–O interatomic lengths (R Ni–O ∼ 2.04 and 2.46 Å), an edge-sharing (R Ni–Fe ∼3.04 Å) and a corner-sharing (R Ni–Fe ∼ 3.56 Å) complex. The R Ni–O ∼ 2.46 Å bond length is not indicative of Ni substituting for Fe in goethite, nor is it associated with single or multiple scattering events in NiO, Ni(OH)2 or Ni substituting for Fe in Fe oxides. The corresponding Ni K-edge EXAFS results for 340/400°C and 800°C heated samples (i.e. hematite) were very similar. Four distinct metal neighbours correspond to Fe/Ni in face-sharing (R Ni–Fe ∼ 2.87–2.91 Å) and three different corner-sharing complexes (R Ni–Fe ∼ 3.37– 3.41 Å, R Ni–Fe ∼ 3.62– 3.64 Å and R Ni–Fe ∼ 3.92– 4.09 Å) represent Ni substituting for Fe in hematite. The fourth shell is indicative of an inner sphere surface complex. EFTEM maps for Ni in goethite are consistent with the formation of a surface complex as they provide evidence for clustering of Ni on the surface of neoformed hematite crystals. There was no evidence from EXAFS or SXRD supporting the formation of discrete Ni phases (e.g. NiO) as a result of shock heating. Therefore, for Ni-goethites subjected to shock heating at 800°C (i.e. high-temperature dehydroxylation to hematite), most of the Ni is retained in the structures of the neoformed hematites, whereas some of the Ni migrates to the surface of the neoformed hematite where it forms a surface complex. During acid dissolution (e.g. heap leaching) of oxide-type lateritic Ni ores, Ni on the hematite surface is more accessible to acid solutions; therefore, these results may provide a basis for more efficient extraction methods for Ni in oxide-type lateritic Ni ores, as well as providing information on the possible redistribution of Ni in heated goethite-rich soils.
HF radar observations of Pc 5 field line resonances in the midnight/early morning MLT sector

On a number of occasions The Johns Hopkins University/Applied Physics Laboratory HF radar at Goose Bay, Labrador, has observed the effects of field line resonances on the drift velocities of irregularities in the F region of the high-latitude ionosphere. These resonances are seen as quasi-monochromatic oscillations in the Doppler velocities measured by the radar. One of the most interesting sets of resonances occurs near midnight MLT and may be associated with shear in the convective flow in the magnetotail. This paper discusses in detail a particularly clear example which shows field line resonances equatorward of a region of shear flow in the early morning sector. Velocity oscillations were recorded at the three discrete frequencies 1.3, 1.95, and 2.6 mHz over a 3 hour period. The motions were predominantly in the geomagnetic east-west direction, indicating north-south electric fields. As expected of field line resonance pulsations, these oscillations had pronounced peaks in their latitudinal power distribution. For the pulsation at 1.95 mHz, a latitudinal phase shift of 180° was observed across the peaks in all the look directions of the radar, and a longitudinal wavelength corresponding to an m value of about 3 was obtained. For the 2.6-mHz pulsation, the phase shifts across the peaks had a variation with look direction that indicated a significant longitudinal as well as latitudinal variation; for this activity we estimate an m value of about 16. The pulsations could occur simultaneously but remained distinct, as the latitude of peak response was observed to vary inversely with the frequency of the pulsation. We interpret these features in terms of field line resonance theory and discuss the possible sources of the pulsation energy.
Nonlinear elasticity of biological basement membrane revealed by rapid inflation and deflation

Significance Basement membranes (BMs) are thin layers of extracellular matrix ubiquitously found in animals surrounding various tissues. As a physical barrier, their mechanical properties are important in maintaining structural integrity of tissues, and their permeabilities are essential for molecule exchange and internal cell activities. However, due to the lack of direct measurement methods, the physical properties of BMs remain largely unclear, limiting our understanding of BMs in various physiological and pathological processes such as tumor development. Here, we apply pressure-controlled inflation/deflation to measure the stress–strain behaviors of intact BM in situ and to determine the mechanical properties in a model-independent manner. We discover a strong strain-stiffening effect of intact BM, which is essential for preventing its snap-through instability. Basement membrane (BM) is a thin layer of extracellular matrix that surrounds most animal tissues, serving as a physical barrier while allowing nutrient exchange. Although they have important roles in tissue structural integrity, physical properties of BMs remain largely uncharacterized, which limits our understanding of their mechanical functions. Here, we perform pressure-controlled inflation and deflation to directly measure the nonlinear mechanics of BMs in situ. We show that the BMs behave as a permeable, hyperelastic material whose mechanical properties and permeability can be measured in a model-independent manner. Furthermore, we find that BMs exhibit a remarkable nonlinear stiffening behavior, in contrast to the reconstituted Matrigel. This nonlinear stiffening behavior helps the BMs to avoid the snap-through instability (or structural softening) widely observed during the inflation of most elastomeric balloons and thus maintain sufficient confining stress to the enclosed tissues during their growth.
Three-Dimensional Modeling of the Ruwais Coastal Area of United Arab Emirates

The coastal flow pattern around a coastal industrial compound in United Arab Emirates has been studied using a three-dimensional hydrodynamic model. The study area is 264 km2 and is partially sheltered from the open sea by salt marshes and islands. Such configuration increases the risk of marine pollution near the industrial site. The region is ecologically diversified and therefore understanding the physical dynamics of the area is important for any assessment work on the impact of industrial and port operations. The utilized model has a size of 232×164 and employs a curvilinear sigma grid system. The setup is nested inside a regional model that covered the entire Arabian Gulf. A k – L module estimates the depth variation of the turbulence field. The model is calibrated against the water level and current data. A sensitivity analysis is done for different wind conditions. The results show that a mean current is developed due to tidal action in the deeper section of the coastal basin. Dominant winds devel.
On the use and validation of mosaic heterogeneity in atmospheric numerical models

The mosaic land modeling approach allows for the representation of multiple surface types in a single atmospheric general circulation model grid box. Each surface type, collectively called “tiles” correspond to different sets of surface characteristics (e.g. for grass, crop or forest). Typically, the tile space data is averaged to grid space by weighting the tiles with their fractional cover. While grid space data is routinely evaluated, little attention has been given to the tile space data. The present paper explores uses of the tile space surface data in validation with station observations. The results indicate the limitations that the mosaic heterogeneity parameterization has in reproducing variations observed between stations at the Atmospheric Radiation Measurement Southern Great Plains field site.
Efficacy and Interindividual Variability in Motor-Cortex Plasticity following Anodal tDCS and Paired-Associative Stimulation

Interindividual response variability to various motor-cortex stimulation protocols has been recently reported. Comparative data of stimulation protocols with different modes of action is lacking. We aimed to compare the efficacy and response variability of two LTP-inducing stimulation protocols in the human motor cortex: anodal transcranial direct current stimulation (a-tDCS) and paired-associative stimulation (PAS25). In two experiments 30 subjects received 1mA a-tDCS and PAS25. Data analysis focused on motor-cortex excitability change and response defined as increase in MEP applying different cut-offs. Furthermore, the predictive pattern of baseline characteristics was explored. Both protocols induced a significant increase in motor-cortical excitability. In the PAS25 experiments the likelihood to develop a MEP response was higher compared to a-tDCS, whereas for intracortical facilitation (ICF) the likelihood for a response was higher in the a-tDCS experiments. Baseline ICF (12 ms) correlated positively with an increase in MEPs only following a-tDCS and responders had significantly higher ICF baseline values. Contrary to recent studies, we showed significant group-level efficacy following both stimulation protocols confirming older studies. However, we also observed a remarkable amount of nonresponders. Our findings highlight the need to define sufficient physiological read-outs for a given plasticity protocol and to develop predictive markers for targeted stimulation.
Plate motions recorded in tectonostratigraphic terranes of the Franciscan complex and evolution of the Mendocino triple junction, northwestern California

The Mendocino triple junction area of northern California is underlain by the Coastal belt of the Franciscan complex, flanked on the east by the Central and Eastern belts of the Franciscan Complex. The coastal belt is further divided into three tectonostratigraphic terranes. Upper Cretaceous through middle Miocene rocks included in these terranes were accreted to the North American plate margin partly during normal convergence with the Farallon plate between 49 and 25 Ma at poleward rates of 2 to 5cm /yr, and partly during translation with the Pacific plate between 14 and 2 Ma at poleward rates of 3 to 6cm/yr. The evolution of the triple junction is discussed. -from Authors
BANCO DE SEMENTES DO SOLO, COMO SUBSÍDIO À RECOMPOSIÇÃO DE MATA CILIAR

Soil seed bank as to grant recovery of the riparian forest. The present work objective knows the density and composition of soil seed bank in ciliary areas altered in spill of two springs. The studied springs belong to Capivari river catchment, Itumirim, MG (21o16'S and 44o52 ' W). The samples of soils were collected in the end of the rainy station (May of 2003). Were 18 soil samples were collected for the two environments to the depth of 5 cm. was calculated the density of seeds and the curve of germination of the soil seed bank, for method of seedling emergency. It was also made the determination of the floristic composition based mainly on the morphology of the seedling. After a period of 210 days of evaluation obtained a density of 2332 seed/m 2 was observed in the environment I and 1896 seed/m 2 , for the environment. However, it was verified that the bank of seeds of the two appraised East was just composed of ruderal species. Such results evidence the need of human intervention in the process of restoration of the ciliary vegetation in spill of this spring, through the artificial regeneration of the arboreal species. Keyword: Seed bank; spring and restoration of the vegetation.
Gene Expression Responses to FUS, EWS, and TAF15 Reduction and Stress Granule Sequestration Analyses Identifies FET-Protein Non-Redundant Functions

The FET family of proteins is composed of FUS/TLS, EWS/EWSR1, and TAF15 and possesses RNA- and DNA-binding capacities. The FET-proteins are involved in transcriptional regulation and RNA processing, and FET-gene deregulation is associated with development of cancer and protein granule formations in amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and trinucleotide repeat expansion diseases. We here describe a comparative characterization of FET-protein localization and gene regulatory functions. We show that FUS and TAF15 locate to cellular stress granules to a larger extend than EWS. FET-proteins have no major importance for stress granule formation and cellular stress responses, indicating that FET-protein stress granule association most likely is a downstream response to cellular stress. Gene expression analyses showed that the cellular response towards FUS and TAF15 reduction is relatively similar whereas EWS reduction resulted in a more unique response. The presented data support that FUS and TAF15 are more functionally related to each other, and that the FET-proteins have distinct functions in cellular signaling pathways which could have implications for the neurological disease pathogenesis.