Miracle-dz/UniFAR-FTUs huggingface dataset:
THE EFFECTS OF DIFFERENT DIETS ON THE FATTY ACID PROFILE OF THE FAIRY SHRIMP CHIROCEPHALUS RUFFOI COTTARELLI & MURA, 1984 (BRANCHIOPODA, ANOSTRACA)

Nauplii of the anostracan Chirocephalus ruffoi were grown until sexual maturity under standard laboratory conditions (18 +/- 1 C; 12L/12D; 1600 lux light intensity) and fed ad libitum once a day on either an alga (Selenastrum capricornutum), baker's yeast, or a formulated diet. Both parents and cysts, as well as feeds were analysed for their fatty acid profiles and compared with wild adults and cysts. Significant differences (P < 0:05) were recorded among the different treatments. As far as total lipid content is concerned, a correlation was found between feeds and adult shrimps as well as between the latter and the cysts they produced during the different feeding tests. Significant differences (P < 0:05) were also recorded from a qualitative point of view. The results obtained provided evidence that the fatty acid profile of both fairy shrimps and cysts can be manipulated in order to improve their nutritional effectiveness, as it was previously shown in the case of the brine shrimp Artemia (Millamena et al., 1988; Lavens et al., 1989; Navarro & Amat, 1992) and of two other freshwater anostracan species (Mura et al., 1997a). Des nauplii de l'anostrace Chirocephalus ruffoi ont ete eleves jusqu'a maturite sexuelle dans des conditions standard de laboratoire (18 +/- 1 C, 12L/12D, 1600 lux d'intensite lumineuse) et nourris a volonte une fois par jour, soit avec une algue (Selenastrum capricornutum), soit avec de la levure de biere, soit avec une formule composee. Les adultes et les oeufs de duree, ainsi que les aliments, ont ete ensuite analyses quant a leur teneur et composition en acides gras et compares aux adultes et aux oeufs de duree sauvages. Des differences significatives (P < 0,05) ont ete notees suivant les differents traitements. En ce qui concerne le contenu total en lipides, une correlation a ete trouvee entre les aliments et les adultes ainsi qu'entre ces derniers et les oeufs de duree qu'ils produisent au cours des differents tests de nutrition. Des differences significatives (P < 0,05) ont ete observees d'un point de vue qualitatif. Les resultats obtenus mettent en evidence que le profil d'acides gras a la fois des anostraces et de leurs oeufs peut etre manipule dans le but d'ameliorer l'efficacite de la nutrition, comme il avait ete montre dans le cas d' Artemia (cf. Millamena et al., 1988; Lavens et al., 1989; Navarro & Amat, 1992) et de deux autres especes d'anostraces des eaux douces (Mura et al., 1997a).
Collagen from jumbo squid fin: extracting conditions and influence of the protease system on collagen hydrolysate antioxidant activity

The optimal alkaline and acid conditions for insoluble collagen extraction from jumbo squid fin (JSF) were established by factorial analysis. Dependent variable: protein concentration; independent variables: NaOH and HCl concentrations. The antioxidant properties of JSF collagen hydrolysates obtained by two protease systems were studied. Moreover, jumbo squid skin (JSS) collagen was obtained and hydrolysated under optimal conditions. The optimal extraction condition was 0.5 M NaOH followed by 0.2 M HCl. Collagen α-chains were detected in both JSF and JSS. Collagen β-component was detected only in JSS. JSS collagen showed higher levels of polar and hydrophobic amino acids. The JSF hydrolysates produced by subtilisin showed a lower degree of hydrolysis and higher antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), and oxygen radical absorbance capacity-fluorescein (ORACFL)) than those produced by a mixture of trypsin, chymotrypsin, and peptidase. The JSS hydrolysates showed a higher antioxidant capacity. We have thus established a suitable process to improve the utility of JSF.
Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non-CpA sites 1 1 Edited by J. Karn

We present the first in vitro study investigating the catalytic properties of a mammalian de novo DNA methyltransferase. Dnmt3a from mouse was cloned and expressed in Escherichia coli. It was shown to be catalytically active in E. coli cells in vivo. The methylation activity of the purified protein was highest at pH 7.0 and 30 mM KCl. Our data show that recombinant Dnmt3a protein is indeed a de novo methyltransferase, as it catalyzes the transfer of methyl groups to unmethylated substrates with similar efficiency as to hemimethylated substrates. With oligonucleotide substrates, the catalytic activity of Dnmt3a is similar to that of Dnmt1: the Km values for the unmethylated and hemimethylated oligonucleotide substrates are 2.5 μM, and the kcat values are 0.05 h−1 and 0.07 h−1, respectively. The enzyme catalyzes the methylation of DNA in a distributive manner, suggesting that Dnmt3a and Dnmt1 may cooperate during de novo methylation of DNA. Further, we investigated the methylation activity of Dnmt3a at non-canonical sites. Even though the enzyme shows maximum activity at CpG sites, with oligonucleotide substrates, a high methylation activity was also found at CpA sites, which are modified only twofold slower than CpG sites. Therefore, the specificity of Dnmt3a is completely different from that of the maintenance methyltransferase Dnmt1, which shows a 40 to 50-fold preference for hemimethylated over unmethylated CpG sites and has almost no methylation activity at non-CpG sites.
Succession in lichen vegetation on Scots pine stumps

Permanent plot observations ascertain successional changes in lichen vegetation on cut surfaces of Scots pines. After about 11 years the pioneer community of Hypogymnia physodes and Evernia prunastri is succeeded by the Cladonietum glaucae Daniels1983. The succesion is considered directional. In the course of the time wood decay increases
Induction of apoptosis in human and rat glioma by agonists of the nuclear receptor PPARγ

Malignant astrocytomas are among the most common brain tumours and few therapeutic options exist. It has recently been recognized that the ligand‐activated nuclear receptor PPARγ can regulate cellular proliferation and induce apoptosis in different malignant cells. We report the effect of three structurally different PPARγ agonists inducing apoptosis in human (U87MG and A172) and rat (C6) glioma cells. The PPARγ agonists ciglitazone, LY171 833 and prostaglandin‐J2, but not the PPARα agonist WY14643, inhibited proliferation and induced cell death. PPARγ agonist‐induced cell death was characterized by DNA fragmentation and nuclear condensation, as well as inhibited by the synthetic receptor‐antagonist bisphenol A diglycidyl ether (BADGE). In contrast, primary murine astrocytes were not affected by PPARγ agonist treatment. The apoptotic death in the glioma cell lines treated with PPARγ agonists was correlated with the transient up‐regulation of Bax and Bad protein levels. Furthermore, inhibition of Bax expression by specific antisense oligonucleotides protected glioma cells against PPARγ‐mediated apoptosis, indicating an essential role of Bax in PPARγ‐induced apoptosis. However, PPARγ agonists not only induced apoptosis but also caused redifferentiation as indicated by outgrowth of long processes and expression of the redifferentiation marker N‐cadherin in response to PPARγ agonists. Taken together, treatment of glioma cells with PPARγ agonists may hold therapeutic potential for the treatment of gliomas.
The Role of Aromatase and Castration on Spatial Learning and Memory Changes by Nandrolone

Materials and Methods: This study used the Morris water maze to evaluate spatial learning and memory. The experimental groups received DMSO as control groups and different doses of nandrolone (10, 30 and 60 μg/ 2.5 μL), anastrozole (2.5, 5 and 10 μg/ 2.5μL), and anastrozole (2.5 μg/ 2.5 μL) + nandrolone (60 μg/ 2.5 μl) all days before the training. The rats of ninth and tenth groups were castrated and treated with 2.5 μL of DMSO and nandrolone (60 μg), respectively for 4 days.
Effect of sodium arsenite on spermatogenesis,plasma gonadotrophins and testosterone in rats

Aim:To investigate the effect of arsenic on spermatogenesis.Methods:Mature(4 months old)Wistar rats were intraperitoneally administered sodium arsenite at doses of 4,5 or 6mg·kg^-1·day^-1 for 26 days.Different varieties of germ cells at stage Ⅶ seminiferous epithelium cycle,namely,type A spermatogonia(ASg),preleptotene spermatocytes(pLSc),midpachytene spermatocytes(mPSc) and step 7 spermatids(7Sd) were quantitatively evaluated, along with radioimmunoassay of plasma follicle-stimulating hormone(FSH),lutuneizing hormone(LH),testosterone and assessment of the epididymal sperm count.Results:In the 5 and 6 mg/kg groups,there were significant dosedependent decreases in the accessory sex organ weights,epididymal sperm count and plasma concentrations of LH,FSH and testosterone with massive degeneration of all the germ cells at stage Ⅶ,The changes were insignificant in the 4 mg/kg group.Conclusion:Arsenite has a suppressive influence on spermatogenesis and gonadotrophin and testosterone release in rats.
Cow urine efficacy against stem borers and cost benefit in soybean production

Field trials were conducted in kharif season for evaluating cow urine efficacy against stem borers and cost benefit in soybean production in comparison to conventional insecticide (chlorpyrifos) and biopesticide (Dipel). Spraying of chemicals was done three times (30, 50 and 70 days after sowing) during the experimental period. Observations on percent infestation of girdle beetle and stem fly was recorded. The plant infestation of Girdle beetle, Obereopsis brevis, was significantly reduced in lower concentrations of cow urine (15, 10 and 5%) treated plots at 7 days after third spray. Stem fly, Melanagromyza sozae, infestation was significantly less at higher levels of cow urine (75 and 100%) than control. There was reduction in percent stem tunneling in almost all levels of cow urine, Chlorpyrifos and Dipel. The yield data indicates that cow urine at 25, 50 and 75% concentration and Chlorpyrifos have significantly higher yield than control. Highest cost benefit ratio (1: 18.9) was obtained from cow urine 5%. However, highest additional yield value of Rs. 6520 per hectare was obtained with cow urine 50%.
Studies Conducted on Entrepreneurship in Science Education: Thematic Review of Research

In recent years in many educational curriculums, entrepreneurship has attracted much attention as a concept, from primary education to higher education. In this sense, attention is given to the concept of entrepreneurship that is included in both national and international science education curriculums. In this research, the aim was to investigate studies conducted on entrepreneurship in science education in terms of their general characteristics (publication year, number of authors, publication type) and characteristics of their content (purpose, method, data collection tool, sample type, conclusion and suggestions). As a result of the research, it is noteworthy that studies have been published in recent years in general. On the other hand, it was identified that there are studies much more published as literature review, document analysis, theoretical. In some research, it was seen that the teaching model, curriculum and modules are developed and integrated within science education to promote entrepreneurship. In addition, it is observed that in most of the studies have been reached positive results about the concept of entrepreneurship should be integrated with science education. Depending on the results, it should be said that teaching curriculums can be designed and implemented that are likely to improve students' entrepreneurial skills in science courses.
Discovery of gene interactions in regulatory networks using genomic data mining and computational intelligence methods

H έλeυση ικανών υπολογιστικών eργαλeίων για την μeλέτη της γeνομικής ακολουθίας και της eρeυνητικής βιοτeχνολογίας υψηλής ανάλυσης, οδήγησe σe μια τeράστια πρόοδο στις eπιστήμeς ζωής. Μeταξύ των πιο σημαντικών καινοτομιών eίναι η τeχνολογία μικροσυστοιχιών. H τeχνολογία αυτή eπιτρέπeι την ποσοτικοποίηση της έκφρασης χιλιάδων γονιδίων ταυτόχρονα, μeτρώντας τον υβριδισμό από έναν ιστό eνδιαφέροντος έως σe δeίγματα σe μικρό γυαλί η σe πλαστικά τσιπ. Πριν ξeκινήσουμe την έρeυνα πάνω στις μικροσυστοιχίeς eίναι σημαντικό να θυμόμαστe ότι τα χαρακτηριστικά των δeδομένων αυτής πeριλαμβάνουν αρκeτό ποσό θορύβου και ένα μη τυπικό αριθμό διαστάσeων (το οποίο καθιστά δύσκολη την χρήση κλασσικών στατιστικών μeθόδων – μέγeθος δeίγματος σe δωδeκάδeς και μέγeθος χαρακτηριστικών σe χιλιάδeς η δeκάδeς η eκατοντάδeς). Eπομένως, ο κύριος στόχος αυτής της διδακτορικής eργασίας eίναι η ανάπτυξη ικανών υπολογιστικών μeθόδων και αλγόριθμων έτσι ώστe να eξάγουν πολύτιμη βιολογική γνώση από τον συγκeκριμένο τύπο δeδομένων. Eφαρμογές της τeχνολογίας μικροσυστοιχιών σαν ένα eργαλeίο για την ανάλυση έκφρασης γονιδίων ξeκινούν από την eύρeση και απόδοση λeιτουργικών κατηγοριών για γονίδια άγνωστης βιολογικής λeιτουργικότητας (βασισμένη στην ανάλυση των γονιδίων ήδη eδραιωμένου βιολογικού ρόλου) έως την ακριβή και πρώιμη διάγνωση διαφορeτικών κακοήθων όγκων. Όμως ο κύριος στόχος της υπολογιστικής ανάλυσης της έκφρασης γονιδίων eίναι η eξαγωγή ρυθμιζόμeνης γνώσης στο γeνeτικό eπίπeδο το οποίο μπορeί να χρησιμοποιηθeί ώστe να παρέχeι μία eυρύτeρη κατανόηση της λeιτουργίας πολύπλοκων κυτταρικών συστημάτων. Σe αυτή την κατeύθυνση, το να αναδeικνύeις τις δομές ρυθμιστικών δικτύων βασισμένων στην έκφραση γονιδίων γίνeται καίριο έργο. Η διδακτορική διατριβή συνeισφέρeι στο πλαίσιο για την ανακάλυψη βιολογικά λeιτουργικών κατηγοριών γονιδίων βασισμένη στην συνeργία της ΙCA και της δυναμικού βασισμένου στη SOM ομαδοποίηση αλγορίθμου η οποία μe ακρίβeια βρίσκeι ομάδeς γονιδίων που συν-ρυθμίζονται eνώ παράλληλα αναγνωρίζeι eνδιαφέροντα ρυθμιστικά σήματα μέσα στα δeδομένα μe τη βοήθeια της ΙCA αποδόμησης. Eπίσης, προσανατολιζόμαστe στην eύρeση του μοριακού χαρακτηρισμού διαφορeτικών τύπων όγκων χρησιμοποιώντας το προφίλ της γονιδιακής έκφρασης, βασισμένο σe ένα σύνολο κατηγοριοποιητών οι οποίοι eκπαιδeύτηκαν σeιριακά σe eπανασταθμισμένeς παραλλαγές των δeδομένων. Ο αλγόριθμος, γνωστός και σαν boosting, έχeι προσαρμοστeί στις ιδιαιτeρότητeς των δeδομένων έκφρασης γονιδίου και eφαρμόζeται σe συνδυασμό μe τα SVMs. Eπιπλέον, eξeτάζeται η πρωτοποριακή τeχνική της eύρeσης προβλέψιμων τιμών των οποίων οι υπογραφές eίναι σημαντικές για τον χαρακτηρισμό φαινότυπου. Τeλικά, η παρούσα διδακτορική διατριβή παρουσιάζeι μια μέθοδο που αναπτύχθηκe για αντίστροφα μηχανικά eλeγχόμeνα από γονίδια νeυρωνικά δίκτυα βασισμένα σe αναδρομικά νeυρωνικά δίκτυα τύπου fuzzy, τα οποία αξιοποιούν τα πλeονeκτήματα των μοντέλων τύπου fuzzy σe βάση eπeξηγηματικότητας αποτeλeσμάτων, και αυτών των νeυρωνικών δικτύων σe βάση υπολογιστικής δύναμης και ικανότητας πρόβλeψης χρονοσeιρών.
Ferns of Malaysian rain forest: a journey through the fern world

Ferns are seedless vascular plants which differ from gymnosperms and flowering plants as they do not have either seeds or flowers but reproduce via spores. They have many features similar to mosses and algae but are usually differentiated from these simpler plants as they have xylem and phloem for the conduction of water and food materials. They are most commonly found in moist, shady forest, crevices in rock faces, and also grow in arctic and alpine tundra, saline mangrove swamps, semiarid deserts and on coastal rocks. The greatest number of fern species can be found in the tropics of both hemispheres. It was estimated that about 10500-11300 of the fern species have been described and 1165 species were recorded in the tropical rain forest of Malaysia. This lecture discusses the morphological diversity, anatomy and cytology of Malaysian rain forest ferns. Selected research works on morphology, anatomy and cytology of these ferns are reviewed. The economic importance of ferns as food, medicine, fibre, craft, fuel and building materials is also discussed. In addition, a number of comprehensive studies on the chemistry and biological activities in ferns are also reviewed. Apart from exploitation of fern taxa for their significant scientific documentation and economic value, the awareness on their conservation status is also emphasized.
GROWTH AND WOOD DENSITY RESPONSES TO LATER-AGE FERTILIZER APPLICATION IN PINUS RADIATA

Growth or ring width responses to nitrogen, phosphorus and the two combined fertilizers were significant over five out of seven years that followed treatment (post-treatment period) in thinned, mid-rotation radiata pine (Pinus radiata D. Don). Responses were similar between nitrogen and phosphorus treatments and largest in the nitrogen plus phosphorus (NP) treatment. Fertilizer increased the proportion of wood formed in mid-rotation relative to juvenile wood thereby increasing tree density in comparison to the control. Fertilizers, other than nitrogen, significantly lowered density compared to the control in the posttreatment period only. Density was higher in nitrogen than phosphorus and similar between phosphorus and NP. Density responses lasted for up to 5 years and were inconsistent between treatments. Ring width and density were weakly correlated. Ring width and density responded differently to climatic variables within and between sites, signalling independent physiological controls and highlighting the importance of site specific differences.
Vanadium Compounds: Chemistry, Biochemistry, and Therapeutic Applications

Vanadium is now known to be one of the more biologically important transition elements. This volume focuses on vanadium chemistry and biochemistry and summarizes the role of vanadium in treating diabetes, concluding with a discussion of a human trial study. The volume looks in particular at model compounds that mimic the chemistry of nitrogenases as haloperoxidases. It also uncovers evolutionary aspects of haloperoxidases while examining structural and sequence relationships between these compounds and acid phosphatases. Aspects of the essentiality, toxicology, and pharmacology are discussed along with insulin-mimetic compounds and their influences in cell cultures.
Genetic regulation of chloroplast biogenesis in Arabidopsis

Chloroplast biogenesis is a complex developmental process that entails the conversion proplastids found in leaf meristems or etioplasts from dark grown seedlings, into fully functional, mature chloroplasts. Variegation mutants are excellent tools using which one can gain access into the poorly understood mechanisms governing chloroplast biogenesis. The Rodermel lab has been interested in characterizing the immutans (im) variegation mutant of Arabidopsis, one of the best-characterized chloroplast biogenesis mutants that defines the gene for the Plastid Terminal Oxidase (PTOX). Over the years, we have focused our efforts in identifying second-site genetic suppressors of immutans to obtain mutants that can rescue the variegation phenotype in the im background by either replacing or bypassing the need for PTOX. My research work in the Rodermel lab has been primarily focused on the characterization of bypass suppressors of immutans. Two new bypass suppressors of immutans have been identified – gigantea and ef1A, both of which offer distinct photosynthetic compensatory mechanisms by which they rescue the variegation phenotype associated with immutans. gigantea suppresses variegation in immutans phenotype of immutans during the late stages of plant development through a complex signal transduction pathway involving crosstalk with hormones like cytokinins and GA. ef1A, instead, functions to delay chloroplast biogenesis in the im and hence rescues variegation in the im background. Suppression of the plastid defect in both imgi and imef1A is likely caused by a relaxation of excitation pressures in developing plastids by factors contributed either directly or indirectly by gi and ef1A.
KOMUNIKASI INTERPERSONAL GURU TERHADAP MURID PENYANDANG AUTISME DALAM PROSES BELAJAR MENGAJAR:Studi kasus guru terhadap murid penyandang autisme di Richmond Homeschooling

Penelitian mengenai komunikasi interpersonal guru terhadap murid penyandang autis didasari oleh peningkatan jumlah anak di Indonesia yang mengalami spektrum autis setiap tahunnya. Keterbatasan komunikasi interpersonal yang dialami oleh penderita autisme membatasi mereka berinteraksi dengan lingkungan sekitarnya dalam beradaptasi, sedangkan manusia sebagai makluk sosial tidak dapat hidup tanpa berkomunikasi dengan yang lainnya. Tujuan dari penelitian ini membahas penerapan komunikasi interpersonal guru terhadap murid penyandang autisme yang menekankan pada proses belajar mengajar di sekolah. Bentuk penelitian yang dipilih adalah pendekatan kualitatif dengan metode penelitian studi kasus. Richmond Homeschooling dipilih sebagai tempat penelitian karena implementasi kurikulum yang berlaku dalam sekolah tersebut tidak menggunakan kurikulum khusus seperti yang biasanya diterapkan dalam sekolah-sekolah anak autis pada umumnya. Hasil dari penelitian ini menunjukkan bahwa guru dalam menerapkan komunikasi interpersonal terhadap murid autisme harus memaksimalkan keseluruhan aspek dalam berkomunikasi baik verbal dan nonverbal. Aspek keterbukaan, empati, sikap mendukung, rasa positif, dan kesetaraan yang terdapat dalam penerapan komunikasi interpersonal dijalankan oleh guru terhadap murid autisme di Richmond Homeschooling sebagai bentuk mendekatkan diri dengan murid autisme. Bentuk penerapan komunikasi verbal dan nonverbal juga diterapkan dengan maksimal oleh guru dalam proses belajar mengajar. ---Research on interpersonal communication of teachers to students with autism is based on the increasing number of children in Indonesia who experience the autism spectrum each year. The limitations of interpersonal communication experienced by autism people limit them to interact with the surrounding environment in adapting, whereas human beings as social beings cannot live without communicating with others. The purpose of this study discusses the application of teacher interpersonal communication to students with autism who emphasize the process of teaching and learning in school. The selected research is qualitative approach with case study research method. Richmond Homeschooling was chosen as a place of study because the curriculum implementation that prevails in the school does not use the special curriculum as it is usually applied in schools of children with autism in general. The results of this study indicate that teachers in applying interpersonal communication to students with autism should maximize all aspects of communicating both verbal and nonverbal. Aspects of openness, empathy, supportiveness, positiveness, and equality that exist in the application of interpersonal communication run by teachers to autism students at Richmond Homeschooling as a form of getting closer to autism students. The form of application of verbal and nonverbal communication is also applied maximally by teachers in teaching and learning process.