Miracle-dz/UniFAR-FTUs huggingface dataset:
A Simple 3D Cell Culture Method for Studying the Interactions between Human Mesenchymal Stromal/Stem Cells and Patients Derived Glioblastoma

Simple Summary Glioblastoma (GBM) remain an incurable disease despite important efforts to find efficient treatments. GBM are heterogeneous, complex, and plastic tumors and as such it is expected that personalized treatments should be the key to therapeutic success. It is thus of the utmost importance to design new methods to mirror the GBM progression and response to treatments for each patient. Our results show that the co-culture of primary cultures derived from GBM patients with cancer associated fibroblasts derived from mesenchymal stem cells exhibit in vitro growth and morphology close to that found in vivo with patients. Abstract We have developed a 3D biosphere model using patient-derived cells (PDCs) from glioblastoma (GBM), the major form of primary brain tumors in adult, plus cancer-activated fibroblasts (CAFs), obtained by culturing mesenchymal stem cells with GBM conditioned media. The effect of MSC/CAFs on the proliferation, cell-cell interactions, and response to treatment of PDCs was evaluated. Proliferation in the presence of CAFs was statistically lower but the spheroids formed within the 3D-biosphere were larger. A treatment for 5 days with Temozolomide (TMZ) and irradiation, the standard therapy for GBM, had a marked effect on cell number in monocultures compared to co-cultures and influenced cancer stem cells composition, similar to that observed in GBM patients. Mathematical analyses of spheroids growth and morphology confirm the similarity with GBM patients. We, thus, provide a simple and reproducible method to obtain 3D cultures from patient-derived biopsies and co-cultures with MSC with a near 100% success. This method provides the basis for relevant in vitro functional models for a better comprehension of the role of tumor microenvironment and, for precision and/or personalized medicine, potentially to predict the response to treatments for each GBM patient.
Higher Dietary Vitamin D Intake Influences the Lipid Profile and hs-CRP Concentrations: Cross-Sectional Assessment Based on The National Health and Nutrition Examination Survey

Background. An unanswered question in the field of nutrition is whether there is an association between vitamin D intake and the lipid profile in adults. We conducted this cross-sectional study in order to investigate the impact of vitamin D intake on the lipid profile of adults in the context of the 2017–2018 National Health and Nutrition Examination Survey (NHANES). Methods. Serum lipids and high-sensitivity C-reactive protein (hs-CRP) concentrations and the Vitamin D intake in 2588 people aged 19 to 70 years was collected using laboratory analysis and 24-h recall, respectively. The one-way ANOVA test was used to compare quantitative variables and the chi-squared test was used to compare qualitative ones. Multivariate logistic regression for three models was performed to assess the odds ratio (OR) of high total cholesterol (TC) (>200 mg/dL), triglycerides (TG) (>150 mg/dL), low-density lipoprotein cholesterol (LDL-C) (>115 mg/dL), high-density lipoprotein cholesterol (HDL-C) (<40 mg/dL) and hs-CRP (>1 mg/l) based on the tertiles of dietary vitamin D (D2 + D3) intake. Results. After adjusting for age, sex, race, body mass index, serum 25-hydroxyvitamin D2, alcohol intake, energy intake, protein intake, carbohydrate intake, fiber intake and fat intake, individuals in the tertile with the highest versus lowest vitamin D intake (>1 mcg/day vs. <0.10 mcg/day) had lower odds of displaying elevated TC, LDL-C and hs-CRP concentrations (OR 0.57; CI: 0.37 to 0.88; P-trend: 0.045, OR 0.59; CI: 0.34 to 1.01; P-trend: 0.025 and OR 0.67; CI: 0.45 to 0.99; P-trend: 0.048, respectively). Based on the results of the logistic regression, no correlation between vitamin D intake and changes in TG or HDL-C values was noted. Conclusions. Our cross-sectional study indicates that higher dietary vitamin D (D2 + D3) intake is associated with lower TC, LDL-C and hs-CRP levels. No relationship between dietary vitamin D intake and TG or HDL-C values was detected. Further large-scale randomized trials are needed to evaluate the actual association between dietary vitamin D intake and the lipid profile.
Sustainable Protocols for Cellulose Nanocrystals Synthesis from Tomato Waste and Their Antimicrobial Properties against Pseudomonas syringae pv. tomato

Nanotechnology is rapidly gaining ground in crop protection, with the growing quest for sustainable nanopesticides and nanocarriers for plant pathogen management. Among them, cellulose nanocrystals (CNC) are emerging as innovative agrofood-waste-derived antimicrobial materials. In this work, new chemical and enzymatic CNC extraction methods from tomato harvest residues were evaluated. The obtained nanomaterials were characterized and tested for their antimicrobial properties on Pseudomonas syringae pv. tomato (Pto), the causal agent of bacterial speck disease on tomato. Both protocols were efficient. The enzymatic extraction method was greener, producing purer CNC at slightly lower yield. The obtained CNC, although they weakly inhibited cell growth and did not promote reactive oxygen species (ROS) formation, provoked bacterial aggregation and the inhibition of biofilm production and swimming motility. Both protocols produced CNC with similar morpho-chemical features, as well as promising antimicrobial activity against plant bacterial pathogens, suggesting their potential role in sustainable crop protection strategies. The new protocols could be a valuable alternative to conventional methods.
Prediction of erythemally effective UVB radiation by means of nonlinear regression model

One of the research programs carried out within the Czech‐Ukrainian scientific co‐operation is the monitoring of global solar and ultraviolet radiation at the Vernadsky Station (formerly the British Faraday Station), Antarctica. Radiation measurements have been made since 2002. Recently, a special attention is devoted to the measurements of the erythemally effective UVB radiation using a broadband Robertson Berger 501 UV‐Biometer (Solar Light Co. Inc., USA). This paper brings some results from modelling the daily sums of erythemally effective UVB radiation intensity in relation to the total ozone content (TOC) in atmosphere and surface intensity of the global solar radiation. Differences between the satellite‐ and ground‐based measurements of the TOC at the Vernadsky Station are taken into consideration. The modelled erythemally effective UVB radiation differed slightly depending on the seasons and sources of the TOC. The model relative prediction error for ground‐ and satellite‐based measurements varied between 9.5% and 9.6% in the period of 2002–2003, while it ranged from 7.4% to 8.8% in the period of 2003–2004. Copyright © 2009 John Wiley & Sons, Ltd.
A high‐resolution submersible oxygen optode system for aquatic eddy covariance

The aquatic eddy covariance technique is increasingly used to determine oxygen (O2) fluxes over benthic ecosystems. The technique uses O2 measuring systems that have a high temporal and numerical resolution. In this study, we performed a series of lab and field tests to assess a new optical submersible O2 meter designed for aquatic eddy covariance measurements and equipped with an existing ultra‐high speed optical fiber sensor. The meter has a 16‐bit digital‐to‐analog‐signal conversion that produces a 0–5 V output at a rate up to 40 Hz. The device was paired with an acoustic Doppler velocimeter. The combined meter and fiber‐optic O2 sensor's response time was significantly faster in O2‐undersaturated water compared to in O2‐supersaturated water (0.087 vs. 0.12 s), but still sufficiently fast for aquatic eddy covariance measurements. The O2 optode signal was not sensitive to variations in water flow or light exposure. However, the response time was affected by the direction of the flow. When the sensor tip was exposed to a flow from the back rather than the front, the response time increased by 37%. The meter's internal signal processing time was determined to be ~ 0.05 s, a delay that can be corrected for during postprocessing. In order for the built‐in temperature correction to be accurate, the meter should always be submerged with the fiber‐optic sensor. In multiple 21–47 h field tests, the system recorded consistently high‐quality, low‐noise O2 flux data. Overall, the new meter is a powerful option for collecting robust aquatic eddy covariance data.
Effects of Species Invasion and Inundation on the Collembola Community in Coastal Mudflat Wetland from the Perspective of Functional Traits

Simple Summary The habitat of wetlands, especially coastal mudflat wetlands, has been seriously threatened by species invasion in recent years, as globalization has progressed worldwide. In this study, we compared the invasive species Spartina alterniflora with the native species Phragmites australis to reveal the effects of invasive plants on species diversity and functional traits of Collembola and explore the adaptation patterns of wetland Collembola in various vegetation and soil depths. We reveal how invasive plants affect coastal mudflat wetlands and offer a reference for conserving wetlands from the perspective of soil fauna. Abstract The group of soil arthropods known as Collembola is characterized by its abundance and sensitivity to environmental changes. They are ideal an species for soil indicators. In order to clarify the effects of species invasion and inundation on the Collembola community in coastal mudflat wetlands, the correlation between the collembolan functional traits and environmental factors was studied in Shanghai Jiuduansha Wetland National Nature Reserve for the first time. Five sample plots, including three vegetations—Spartina alterniflora (an invasive species), Phragmites australis, and Zizania latifolia—were set up following the differences in vegetation types and between high and low tidal flats. Data on the diversity of the Collembolan species and their functional traits were collected and combined with the soil physicochemical properties and vegetation environment factors in different tidal flats. The key findings and conclusions of the study are as follows: a total of 18 species, four families, and three orders make up the obtained Collembola, two species of Proisotoma are dominant species that account for 49.59% and 24.91% of the total, respectively. The maintenance of the species diversity of Collembola is disturbed by the higher conversion efficiency of Spartina alterniflora rather than Phragmites australis with lower organic carbon (C) content and higher total nitrogen (N) content. The primary environmental variables influencing species distribution were the C/N ratio, total N, and bulk soil density. The bulk density of the soil impacts the movement and dispersal of the functional traits. The depth of the soil layer is related to the functional traits of the sensory ability. The analysis of the functional traits and environment is fairly helpful in exploring how species respond to their environment and offers a better explanation for the habitat selection of Collembola.
New (types of) parties and government: the Danish general election 2022

Abstract During the Covid-19 crisis, there was a high level of rallying around the flag for the Social Democratic government. However, support waned with the sense of crisis, and some ‘scandals’ surfaced. The ‘mink scandal’ caused an early election to be called. A high number of parties, 14, competed in the election, and after the election 12 parties are now represented in parliament – a new record number. When comparing the election results of 2019 and 2022, two overall results stand out. First, only one of the two traditional large parties has maintained this status and fragmentation is increased. Second, two new parties made it into the ‘top-5’ in 2022, both formed by former prominent Liberals. The election yielded a narrow victory to the red bloc. Prime Minister Mette Frederiksen was able to stay in office after the election but exchanged her red bloc parliamentary majority for a coalition government with the Liberals and Moderates. Thus, a new type of coalition has emerged: a majority government spanning the usual two blocs, but challenged by opposition from both the right and left.
Effectiveness of Epidural Steroid Injection Depending on Discoradicular Contact: A Prospective Randomized Trial

Lumbar radicular pain is a major public health and economic problem. It is among the most common reasons for professional disability. The most common cause of lumbar radicular pain is intervertebral disc herniation, which results from degenerative disc changes. The dominant pain mechanisms are direct pressure of the hernia on the nerve root and the local inflammatory process triggered by intervertebral disc herniation. Treatment of lumbar radicular pain includes conservative, minimally invasive, and surgical treatment. The number of minimally invasive procedures is constantly increasing, and among these methods is epidural administration of steroids and local anesthetic through a transforaminal approach (ESI TF). The aim of this research was to examine the effectiveness of ESI TF as measured by a visual analog scale (VAS) and the Oswestry Disability Index (ODI), depending on whether there is contact between the herniated intervertebral disc and the nerve root. In both groups of participants, there was a significant reduction in pain intensity, but there was no significant difference between the groups. In the group with disc herniation and nerve root contact, the only significant reduction was in pain intensity (p < 0.001). There were no significant differences in measurements in other domains of the ODI. In the group without disc herniation and nerve contact, there was a significant difference in all domains except weight lifting. In the group without contact, there was significant improvement after 1 month (p = 0.001) and 3 months (p < 0.001) according to the ODI, while there was no significant improvement in the group with contact. In addition, there were no significant differences in the distribution of participants based on the ODI and whether disc herniation and nerve contact was present. The results suggest that transforaminal epidural administration of steroids is a clinically effective method for treating lumbar radicular pain caused by intervertebral disc herniation in people with and without nerve root contact, without significant differences.
Cerebrospinal Fluid and Blood Biomarkers in Patients with Post-Traumatic Disorders of Consciousness: A Scoping Review

(1) Background: Cerebrospinal fluid (CSF) and blood biomarkers are emerging tools used to obtain information on secondary brain damage and to improve diagnostic and prognostic accuracy for patients with prolonged post-traumatic disorders of consciousness (DoC). We synthesized available data from studies evaluating CSF and blood biomarkers in these patients. (2) Methods: A scoping review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews checklist to identify and synthesize data from relevant studies. Studies were identified by PubMed and manual searches. Those involving patients with unresponsive wakefulness syndrome or in a minimally conscious state for >28 days, evaluating CSF or blood biomarkers, and conducted on patients with traumatic brain injuries older than 16 years were included in the review. (3) Results: In total, 17 studies were included. Findings on neurofilament light chain, proteins, metabolites, lipids, amyloid-β, tau, melatonin, thyroid hormones, microtubule-associated protein 2, neuron-specific enolase, and brain-derived neurotrophic factor were included in the qualitative synthesis. (4) Conclusions: The most promising applications for CSF and blood biomarkers are the monitoring of secondary neurodegeneration, support of DoC diagnoses, and refinement of prognoses, although current evidence remains too scarce to recommend such uses of these biomarkers in clinical practice.
Associations between Gendered Family Structures and Adolescent Stress, Loneliness, and Sadness in South Korea

While a large literature connects family environments characterized by access to two married biological parents to better child mental health outcomes, we know less about the mechanisms linking family structure to mental health outcomes for children living in other family structures. While essentialist theory suggests that access to both male and female parents will be an important determinant of child mental health, some research directly comparing single-mother and single-father families found no difference in child outcomes by parent gender, suggesting evidence for more structural theories of gender. However, most of this research uses data from Western countries and seldom extends to examining mental health outcomes. In this paper, we used data from a large, generalizable survey of Korean adolescents (the 2021 Korea Youth Risk Behavior Survey) to compare the mental health of children living in families with two married biological parents, single mothers, and single fathers. Our findings underscore the importance of examining family environments in different contexts.
Quantitative Trait Loci (QTL) Analysis of Seed Protein and Oil Content in Wild Soybean (Glycine soja)

Soybean seeds consist of approximately 40% protein and 20% oil, making them one of the world’s most important cultivated legumes. However, the levels of these compounds are negatively correlated with each other and regulated by quantitative trait loci (QTL) that are controlled by several genes. In this study, a total of 190 F2 and 90 BC1F2 plants derived from a cross of Daepung (Glycine max) with GWS-1887 (G. soja, a source of high protein), were used for the QTL analysis of protein and oil content. In the F2:3 populations, the average protein and oil content was 45.52% and 11.59%, respectively. A QTL associated with protein levels was detected at Gm20_29512680 on chr. 20 with a likelihood of odds (LOD) of 9.57 and an R2 of 17.2%. A QTL associated with oil levels was also detected at Gm15_3621773 on chr. 15 (LOD: 5.80; R2: 12.2%). In the BC1F2:3 populations, the average protein and oil content was 44.25% and 12.14%, respectively. A QTL associated with both protein and oil content was detected at Gm20_27578013 on chr. 20 (LOD: 3.77 and 3.06; R2 15.8% and 10.7%, respectively). The crossover to the protein content of BC1F3:4 population was identified by SNP marker Gm20_32603292. Based on these results, two genes, Glyma.20g088000 (S-adenosyl-l-methionine-dependent methyltransferases) and Glyma.20g088400 (oxidoreductase, 2-oxoglutarate-Fe(II) oxygenase family protein), in which the amino acid sequence had changed and a stop codon was generated due to an InDel in the exon region, were identified.
Comparing cancer risks between radiation and dioxin exposure based on two‐stage model

This study investigates the use of the Moolgavkar two‐stage model to compare the biological and statistical nature of cancer risks from dioxin exposure and from radiation exposure. The two‐stage model is a biologically based stochastic model for carrying out statistical inferences of cancer risks using biologically motivated hypothesis. This article briefly describes the conditional likelihood for the model recently developed by the authors and then presents results obtained from applying it to dioxin dietary data in rats (Kociba et al., 1978) and radiation exposure data in mice. Comparing the results following the two different types of exposures based on the two‐stage model confirms that the radiation exposure increases the initiation rates while the dioxin exposure increases the net‐proliferation rate. The results indicate that the effect of TCDD at 100 ng/kg/day is an approximately 40 per cent increase in the net‐proliferation rate in the intermediate cells. Portier et al. (1996b) and Moolgavkar et al. (1996) used the two‐stage in‐vivo initiation‐promotion model in the rat liver. Measuring liver foci after administration of DEN followed by TCDD as a promoter, they observed an increase in proliferation rate in their initiated cells by 25–35 per cent. This closely agrees with our estimated increase of 40 per cent in the whole animal cancer incidence study of (Kociba et al., 1978). Another statistically important aspect obtained from the study is that the proportional hazards model is appropriate to apply to cancer incidence data following radiation exposure but is not appropriate for cancer risks following dioxin exposure. Copyright © 2003 John Wiley & Sons, Ltd.
2 3 A pr 2 01 7 Existence , proper Pareto reducibility , and connectedness in multi-objective optimization

This paper is divided to two parts. In the first part, we provide elementary proofs for some important results in multi-objective optimization. The given proofs are so simple and short in compared to the existing ones. Also, a Pareto reducibility result is extended from efficiency to proper efficiency. The second part is devoted to the relationships between nonemptiness, Rp ≧ (semi)compactness, external stability and connectedness of the set of nondominated solutions in multi-objective optimization. Furthermore, it is shown that some assumption in an important result, concerning connectedness, is redundant and should be removed.
Ocular Ultrasonography in Healthy Calves with Different Transducers

Simple Summary Ocular ultrasonography for eye examination is seldom performed in cattle. Despite this, some ocular pathologies have detrimental effects on animal welfare. Thus, the knowledge of normal ultrasonographic eye patterns and biometry is helpful for correct diagnosis. In this study, we compared the measurements made with three ultrasound transducers (linear, convex, transrectal gynecological) in field conditions on 20 healthy calves (10 Holstein breed, 10 Piedmontese breed). While both linear and transrectal gynecological transducers can be used for eye examination in the field, widespread application of the transrectal gynecological probe make it the most suitable choice for ocular ultrasonography in field conditions. Abstract Ocular ultrasonography is seldom performed in cattle. Here, we compared three ultrasound probes (linear, convex, transrectal gynecological) for the measurement of eight eye biometry parameters on vertical and horizontal scans. The sample population was 20 healthy calves (n = 10 Holstein, n = 10 Piedmontese breed). Intragroup (same probe for vertical vs. horizontal scanning) and intergroup (different probes measuring the same biometric parameter) comparisons were performed using Student’s t-test and the Mann–Whitney U test. Statistical significance was set at p ≤ 0.05. Intragroup comparison revealed few significant differences. Ultrasound examination with the convex transducer detected more differences than either of the two other probes on vertical (linear six out of eight; transrectal gynecological four out of eight) and horizontal (linear six out of eight; transrectal gynecological six out of eight) scans. Similar results were obtained for both breeds. More non-valuable parameters on the horizontal (77 out of 320, 24%) and the vertical (85 out of 320, 26%) (p ≤ 0.001) scans were obtained with the convex transducer. Both linear transducers were found comparable for ocular ultrasonography in field conditions. However, given its widespread application in the field, the transrectal gynecological transducer may offer veterinarians the added advantage of familiarity and ease-of-use without any additional costs.