Miracle-dz/UniFAR-FTUs huggingface dataset:
Cardiovascular Risk Associated with Interactions among Polymorphisms in Genes from the Renin-Angiotensin, Bradykinin, and Fibrinolytic Systems

Background Vascular fibrinolytic balance is maintained primarily by interplay of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor type 1 (PAI-1). Previous research has shown that polymorphisms in genes from the renin-angiotensin (RA), bradykinin, and fibrinolytic systems affect plasma concentrations of both t-PA and PAI-1 through a set of gene-gene interactions. In the present study, we extend this finding by exploring the effects of polymorphisms in genes from these systems on incident cardiovascular disease, explicitly examining two-way interactions in a large population-based study. Methodology/Principal Findings Data from the population-based PREVEND study in Groningen, The Netherlands (n = 8,138) were analyzed. The effects of the polymorphisms and their interactions on cardiovascular events were analyzed via Cox proportional hazards models. There was no association between five of the six polymorphisms singly and risk of cardiovascular disease. There was a significant main effect for the ACE I/D polymorphism for both dominant and additive coding schemes. There were significant interactions between the following polymorphism pairs even after adjustment for known risk factors: ACE I/D & PAI-1 4G/5G (p = 0.012), BDKRB2 C181T & ACE I/D (p = 0.016), BDKRB2 C58T & ACE I/D (p = 0.025), BDKRB2 exon 1 I/D & AT1R A1166C (p = 0.017), and BDKRB2 C58T & AT1R A1166C (p = 0.015). Conclusions/Significance This study suggests possible interactions between genes from the RA, bradykinin, and fibrinolytic systems on the risk of cardiovascular disease, extending previous research that has demonstrated that interactions among genes from these systems influence plasma concentrations of both t-PA and PAI-1. Further explorations of these interactions are needed.
Mapping of Average Shear Wave Velocity for Bangalore Region: A Case Study

Mapping the shear wave velocity profile is an important part in seismic hazard and microzonation studies. The shear wave velocity of soil in the city of Bangalore was mapped using the Multichannel Analysis of Surface Wave (MASW) technique. An empirical relationship was found between the Standard Penetration Test (SPT) corrected N value ((N1)60cs) and measured shear wave velocity (Vs). The survey points were selected in such a way that the results represent the entire Bangalore region, covering an area of 220 km2. Fifty-eight 1-D and 20 2-D MASW surveys were performed and their velocity profiles determined. The average shear wave velocity of Bangalore soils was evaluated for depths of 5 m, 10 m, 15 m, 20 m, 25 m and 30 m. The sub-soil classification was made for seismic local site effect evaluation based on average shear wave velocity of 30-m depth (Vs30) of sites using the National Earthquake Hazards Reduction Program (NEHRP) and International Building Code (IBC) classification. Mapping clearly indicates that the depth of soil obtained from MASW closely matches with the soil layers identified in SPT bore holes. Estimation of local site effects for an earthquake requires knowledge of the dynamic properties of soil, which is usually expressed in terms of shear wave velocity. Hence, to make use of abundant SPT data available on many geotechnical projects in Bangalore, an attempt was made to develop a relationship between Vs (m/s) and (N1)60cs. The measured shear wave velocity at 38 locations close to SPT boreholes was used to generate the correlation between the corrected N values and shear wave velocity. A power fit model correlation was developed with a regression coefficient (R2) of 0.84. This relationship between shear wave velocity and corrected SPT N values correlates well with the Japan Road Association equations.
“Inflamm‐aging” influences immune cell survival factors in human bone marrow

The bone marrow (BM) plays a key role in the long‐term maintenance of immunological memory. However, the impact of aging on the production of survival factors for effector/memory T cells and plasma cells in the human BM has not been studied. We now show that the expression of molecules involved in the maintenance of immunological memory in the human BM changes with age. While IL‐15, which protects potentially harmful CD8+CD28− senescent T cells, increases, IL‐7 decreases. IL‐6, which may synergize with IL‐15, is also overexpressed. In contrast, a proliferation‐inducing ligand, a plasma cell survival factor, is reduced. IFN‐y, TNF, and ROS accumulate in the BM in old age. IL‐15 and IL‐6 expression are stimulated by IFN‐y and correlate with ROS levels in BM mononuclear cells. Both cytokines are reduced by incubation with the ROS scavengers N‐acetylcysteine and vitamin C. IL‐15 and IL‐6 are also overexpressed in the BM of superoxide dismutase 1 knockout mice compared to their WT counterparts. In summary, our results demonstrate the role of inflammation and oxidative stress in age‐related changes of immune cell survival factors in the BM, suggesting that antioxidants may be beneficial in counteracting immunosenescence by improving immunological memory in old age.
Seismic mapping of a magma chamber beneath the Valu Fa Ridge, Lau Basin

We analyze a dense grid of seismic normal incidence and wide-angle data collected over an active back arc spreading center in the Lau Basin of the southwest Pacific. Our survey area covers the whole of a 35-km-long morphological segment and a small overlapping spreading center. A bright reflector, which is coincident with a velocity inversion at a depth of 3.2 ± 0.2 km below the seafloor, is observed on every one of the 40 across-axis profiles. We interpret this reflector as being the roof of a crustal magma chamber. The widest magma chamber reflector occurs beneath the overlapping spreading center, where it extends up to 4 km, being imaged beneath both ridges and, in places, beneath the overlap basin. Elsewhere the width of the reflector varies between 0.6 and 2.3 ±0.4 km. The narrowest reflectors are observed beneath deviations (devais) of the ridge axis. In addition to being observed on each of the kilometer-spaced across-axis profiles the reflector is also seen as a continuous event for at least 10 km on a profile along the ridge axis. The reflection coefficient of the event is between −0.34 and −0.65. This requires the melt at the top of the chamber to have a P wave velocity less than 2.7 km/s if the interface is planar or less than 3.8 km/s if the interface is layered.
A physical explanation for an observed area‐slope‐elevation relationship for catchments with declining relief

A general relationship between the contributing area, slope, and mean elevation of catchments with relief declining after a tectonic uplift event is presented. This relationship is based on the continuity equation for runoff and erosion processes in the catchment. The key hypothesis underlying this relationship is that as a catchment declines, the nondimensionalized catchment approaches a constant form. This hypothesis is verified for computer simulated catchments. The area-slope-elevation relationship covers several cases: catchments declining toward a peneplain; catchments declining from a high slope dynamic equilibrium (resulting from a high rate of tectonic uplift) to a low slope one (resulting from a lower rate of tectonic uplift); and catchments declining from an elevated initial condition, as, for example, in the decline of a mine spoil heap. A previously published relationship between slope and area for catchments in dynamic equilibrium and based on runoff and erosion physics is shown to be a special case of this general relationship. The new area-slope-elevation relationship is compared with data from simulated catchments and a field catchment. It is thus shown that for declining catchments the area-slope-elevation relationship is a good predictor of catchment form for catchments with declining relief. It is argued that the slope-area-elevation relationship is sufficient, with the planiform drainage pattern, to completely define the elevation properties of the catchment such as, for instance, the hypsometric curve.
Unraveling the Regulatory Mechanisms Underlying Tissue-Dependent Genetic Variation of Gene Expression

It is known that genetic variants can affect gene expression, but it is not yet completely clear through what mechanisms genetic variation mediate this expression. We therefore compared the cis-effect of single nucleotide polymorphisms (SNPs) on gene expression between blood samples from 1,240 human subjects and four primary non-blood tissues (liver, subcutaneous, and visceral adipose tissue and skeletal muscle) from 85 subjects. We characterized four different mechanisms for 2,072 probes that show tissue-dependent genetic regulation between blood and non-blood tissues: on average 33.2% only showed cis-regulation in non-blood tissues; 14.5% of the eQTL probes were regulated by different, independent SNPs depending on the tissue of investigation. 47.9% showed a different effect size although they were regulated by the same SNPs. Surprisingly, we observed that 4.4% were regulated by the same SNP but with opposite allelic direction. We show here that SNPs that are located in transcriptional regulatory elements are enriched for tissue-dependent regulation, including SNPs at 3′ and 5′ untranslated regions (P = 1.84×10−5 and 4.7×10−4, respectively) and SNPs that are synonymous-coding (P = 9.9×10−4). SNPs that are associated with complex traits more often exert a tissue-dependent effect on gene expression (P = 2.6×10−10). Our study yields new insights into the genetic basis of tissue-dependent expression and suggests that complex trait associated genetic variants have even more complex regulatory effects than previously anticipated.
Some aspects of life strategies of Early Palaeozoic rugose corals

Examination of some well-preserved specimens of Silurian rugose corals from Gotland reveals that the following categories of life strategies can be distinguished: ambitopic, liberosessile, fixosessile, rhizosessile and possibly limited vagile. Most solitary rugose corals appear to have been liberosessile and are characterized by initial attachment to a small sediment grain but subsequently becoming recumbent on a soft substrate. Detailed studies of Phaulactis angelini, Holophragma calceoloides, Laccophyllum lindstroemi, Rhegmaphyllum conulus and Rhabdocyclus ocksaroensis n. sp. , and comparison with earlier described species, provide some new aspects on different life strategies for solitary corals.
A Study of Properties of Concrete Making Partial Replacement of Cement by Ceramic waste Powder

1M.Tech student, Department of Civil Engineering, Sree Narayana Institute of Technology, Adoor, Kerala 2Assistant professor, Department of Civil Engineering, Sree Narayana Institute of Technology, Adoor, Kerala ---------------------------------------------------------------------***--------------------------------------------------------------------Abstract Concrete is the most widely used construction material all over the globe next to water. The present challenge for civil engineers is to produce large volume, good performance, durable and sustainable concrete at lowest possible cost. In the ceramic industry, nearly 15%-30% production goes as waste. The replacement of cement with Ceramic Waste Powder (CWP) produces a substantial modification in compressive strength, making them suitable for the fabrication of eco-efficient concrete. Using M30 grade concrete, ceramic powder concrete produced by replacing cement in 5%,10%,15%, 20%,25%,and 30% by weight of cement. Concrete mixtures were produced, tested and compared in terms of compressive strength, split tensile strength and flexural strength to the conventional concrete These tests were carried out to evaluate the mechanical properties after 7, 14 and 28 days. Results showed that 15 % replacement of cement by ceramic powder makes a considerable increase in compressive strength, split tensile strength and flexural strength.
Two‐dimensional physically based finite element runoff model for small agricultural watersheds: II. Model testing and field application

Mathematical models are useful analysis tools to understand problems in watersheds associated with runoff, and to find solutions through land use changes and best management practices. However, before a model is applied in the field, it must be tested and checked to ensure that the model represents the real world adequately. In this paper, a two‐dimensional physically based finite element runoff model ROMO2D has been verified and validated by comparing the model output with analytic solution under simplified conditions, published data, and field measurements. Calibration of the model was done manually through a multi‐objective calibration procedure, using observed field data. Before going for field validation/application of ROMO2D, analysis was carried out to determine the optimum number of finite elements into which the watershed should be discretized and the size of the time step. A sensitivity analysis of the model was performed using the observed values of watershed parameters. The model was applied to a 1·45 ha agricultural watershed located in the Shiwalik foothills (India) to simulate runoff. The results demonstrated the potential of the model to simulate runoff from small agricultural watersheds for individual storm events with reasonable accuracy. Copyright © 2008 John Wiley & Sons, Ltd.
Insulin receptor substrate 2 (IRS2) deficiency delays liver fibrosis associated with cholestatic injury

ABSTRACT Insulin receptor substrate 2 (IRS2) is a key downstream mediator of insulin and insulin-like growth factor 1 (IGF1) signalling pathways and plays a major role in liver metabolism. The aim of this study was to investigate whether IRS2 had an impact on the hepatic fibrotic process associated with cholestatic injury. Bile duct ligation (BDL) was performed in wild-type (WT) and Irs2-deficient (IRS2KO) female mice. Histological and biochemical analyses, together with fibrogenic and inflammatory responses were evaluated in livers from mice at 3, 7 and 28 days following BDL. We also explored whether activation of human hepatic stellate cells (HSCs) induced by IGF1 was modulated by IRS2. IRS2KO mice displayed reduced disruption of liver histology, such hepatocyte damage and excess deposition of extracellular matrix components, compared with WT mice at 3 and 7 days post-BDL. However, no histological differences between genotypes were found at 28 days post-BDL. The less pro-inflammatory profile of bile acids accumulated in the gallbladder of IRS2KO mice after BDL corresponded with the reduced expression of pro-inflammatory markers in these mice. Stable silencing of IRS2 or inhibition of ERK1/2 reduced the activation of human LX2 cells and also reduced induction of MMP9 upon IGF1 stimulation. Furthermore, hepatic MMP9 expression was strongly induced after BDL in WT mice, but only a slight increase was found in mice lacking IRS2. Our results have unravelled the signalling pathway mediated by IGF1R–IRS2–ERK1/2–MMP9 as a key axis in regulating HSC activation, which might be therapeutically relevant for targeting liver fibrosis. Summary: IRS2 is a key mediator of IGF1R signalling in hepatic stellate cell activation in cholestatic liver injury.
Does the performance of five back-associated exercises relate to the presence of low back pain? A cross-sectional observational investigation in regional Australian council workers

Objectives Investigate the relationships between the ability/inability to perform five physical test exercises and the presence or absence of low back pain (LBP). Setting Regional Australian council training facility. Participants Consecutive participants recruited during 39 back education classes (8–26 participants per class) for workers in general office/administration, parks/gardens maintenance, roads maintenance, library, child care and management. Total sample (n=539) was reduced through non-consent and insufficient demographic data to n=422. Age 38.6±15.3 years, range 18–64 years, 67.1% male. Methods Cross-sectional, exploratory, observational investigation. LBP presence was ascertained from a three-response option questionnaire: 0=none/rarely (no) 1=sometimes (some), 2=mostly/always (most). Statistical correlation was performed with the number of the five test exercises the individual successfully performed: (1) extension in lying: 3 s; (2) ‘toilet squat’; feet flat, feet touched: 3 s; (3) full squat then stand up: 5 times; (4) supine sit-up, knees flexed: 10 times; and (5) leg extension, supine bilateral: 10 times. Interventions Nil. Results For the group ‘no-some’, 94.3% completed 4–5 test exercises, while for group ‘With’, 95.7% completed 0–1 test exercises. The relationship between LBP presence and number of exercises performed was highly significant (χ2 (10)=300.61, p<0.001). Furthermore, multinomial logistic regression predicting LBP (0=no, 1=some, 2=most) from the number of exercises completed, substantially improved the model fit (initial-2LL=348.246, final-2LL=73.620, χ2 (2)=274.626, p<0.001). As the number of exercises performed increased, the odds of reporting ‘some LBP’ or ‘most LBP’ dropped substantially (ORs of 0.34 and 0.17, respectively). Conclusion The ability to complete/not complete five test exercises correlated statistically and significantly with a higher LBP absence/presence in a general working population. Training individuals to complete such exercises could facilitate reductions in LBP incidence; however, causality cannot be inferred. Randomised trials are recommended to establish the potential efficacy of exercise-based approaches, considering these five selected exercises, for predicting and managing LBP.
Disentangling Wording and Substantive Factors in the Spiritual Well-Being Scale

We evaluated the extent to which the Spiritual Well-Being Scale (SWBS) may help to meet the need for multidimensional, psychometrically sophisticated measures of spiritual and religious traits. Although the various forms of validity of the scale have, for the most part, been supported by psychometric studies, conflicting evidence surrounding its dimensionality has called into question its structural validity. Specifically, numerous authors have suggested that a more appropriate factor structure for the SWBS includes further substantive factors in addition to the 2 factors that the scale was originally intended to measure. In the current study, we attempted to resolve these debates using a combination of exploratory and confirmatory factor analysis based investigations in the Lothian Birth Cohort, 1921 study. Our analyses suggested that the additional factors suggested in previous studies may not have reflected substantive constructs; but rather, common variance due to methodological factors.
Circadian genes Per1 and Per2 increase radiosensitivity of glioma in vivo

Per1 and Per2 play a key role in regulating the circadian rhythm in mammals. We report here that although both genes were expressed with a circadian rhythm in glioma and normal brain tissue in rats, their expression profiles differed in the two types of tissue. In addition, high expression of Per1 and Per2 in glioma tissue was associated with increased sensitivity to x-irradiation. No such sensitizing effect was observed in normal tissue. Our results suggest that Per1 and Per2 expression may increase the efficacy of radiotherapy against glioma by promoting apoptosis.
Uncoupling Lipid Synthesis from Adipocyte Development

Obesity results from the expansion of adipose tissue, a versatile tissue regulating energy homeostasis, adipokine secretion, thermogenesis, and inflammation. The primary function of adipocytes is thought to be lipid storage through lipid synthesis, which is presumably intertwined with adipogenesis. However, during prolonged fasting, adipocytes are depleted of lipid droplets yet retain endocrine function and an instant response to nutrients. This observation led us to question whether lipid synthesis and storage can be uncoupled from adipogenesis and adipocyte function. By inhibiting key enzymes in the lipid synthesis pathway during adipocyte development, we demonstrated that a basal level of lipid synthesis is essential for adipogenesis initiation but not for maturation and maintenance of adipocyte identity. Furthermore, inducing dedifferentiation of mature adipocytes abrogated adipocyte identity but not lipid storage. These findings suggest that lipid synthesis and storage are not the defining features of adipocytes and raise the possibility of uncoupling lipid synthesis from adipocyte development to achieve smaller and healthier adipocytes for the treatment of obesity and related disorders.
Paratransgenic manipulation of a tsetse microRNA alters the physiological homeostasis of the fly’s midgut environment

Tsetse flies are vectors of parasitic African trypanosomes, the etiological agents of human and animal African trypanosomoses. Current disease control methods include fly-repelling pesticides, fly trapping, and chemotherapeutic treatment of infected people and animals. Inhibiting tsetse’s ability to transmit trypanosomes by strengthening the fly’s natural barriers can serve as an alternative approach to reduce disease. The peritrophic matrix (PM) is a chitinous and proteinaceous barrier that lines the insect midgut and serves as a protective barrier that inhibits infection with pathogens. African trypanosomes must cross tsetse’s PM in order to establish an infection in the fly, and PM structural integrity negatively correlates with trypanosome infection outcomes. Bloodstream form trypanosomes shed variant surface glycoproteins (VSG) into tsetse’s gut lumen early during the infection establishment, and free VSG molecules are internalized by the fly’s PM-producing cardia. This process results in a reduction in the expression of a tsetse microRNA (miR275) and a sequential molecular cascade that compromises PM integrity. miRNAs are small non-coding RNAs that are critical in regulating many physiological processes. In the present study, we investigated the role(s) of tsetse miR275 by developing a paratransgenic expression system that employs tsetse’s facultative bacterial endosymbiont, Sodalis glossinidius, to express tandem antagomir-275 repeats (or miR275 sponges). This system induces a constitutive, 40% reduction in miR275 transcript abundance in the fly’s midgut and results in obstructed blood digestion (gut weights increased by 52%), a significant increase (p-value < 0.0001) in fly survival following infection with an entomopathogenic bacteria, and a 78% increase in trypanosome infection prevalence. RNA sequencing of cardia and midgut tissues from paratransgenic tsetse confirmed that miR275 regulates processes related to the expression of PM-associated proteins and digestive enzymes as well as genes that encode abundant secretory proteins. Our study demonstrates that paratransgenesis can be employed to study microRNA regulated pathways in arthropods that house symbiotic bacteria.