Miracle-dz/UniFAR-FTUs huggingface dataset:
Phenotypic analysis of the pediatric immune response to SARS‐CoV‐2 by flow cytometry

Pediatric SARS‐CoV‐2 infection is often mild or asymptomatic and the immune responses of children are understudied compared to adults. Here, we present and evaluate the performance of a two‐panel (16‐ and 17 parameter) flow cytometry‐based approach for immune phenotypic analysis of cryopreserved PBMC samples from children after SARS‐CoV‐2 infection. The panels were optimized based on previous SARS‐CoV‐2 related studies for the pediatric immune system. PBMC samples from seven SARS‐CoV‐2 seropositive children from early 2020 and five age‐matched healthy controls were stained for analysis of T‐cells (panel T), B and innate immune cells (panel B). Performance of the panels was evaluated in two parallel approaches, namely classical manual gating of known subpopulations and unbiased clustering using the R‐based algorithm PhenoGraph. Using manual gating we clearly identified 14 predefined subpopulations of interest for panel T and 19 populations in panel B in low‐volume pediatric samples. PhenoGraph found 18 clusters within the T‐cell panel and 21 clusters within the innate and B‐cell panel that could be unmistakably annotated. Combining the data of the two panels and analysis approaches, we found expected differentially abundant clusters in SARS‐CoV‐2 seropositive children compared to healthy controls, underscoring the value of these two panels for the analysis of immune response to SARS‐CoV‐2. We established a two‐panel flow cytometry approach that can be used with limited amounts of cryopreserved pediatric samples. Our workflow allowed for a rapid, comprehensive, and robust pediatric immune phenotyping with comparable performance in manual gating and unbiased clustering. These panels may be adapted for large multi‐center cohort studies to investigate the pediatric immune response to emerging virus variants in the ongoing and future pandemics.
Changes in perspective needed to forge ‘no‐regret’ forest‐based climate change mitigation strategies

Forest‐based mitigation strategies will play a pivotal role in achieving the rapid and deep net‐emission reductions required to prevent catastrophic climate change. However, large disagreement prevails on how to forge forest‐based mitigation strategies, in particular in regions where forests are currently growing in area and carbon density. Two opposing viewpoints prevail in the current discourse: (1) A widespread viewpoint, specifically in countries in the Global North, favours enhanced wood use, including bioenergy, for substitution of emissions‐intensive products and processes. (2) Others instead focus on the biophysical, resource‐efficiency and time‐response advantages of forest conservation and restoration for carbon sequestration and biodiversity conservation, whilst often not explicitly specifying how much wood extraction can still safeguard these ecological benefits. We here argue for a new perspective in sustainable forest research that aims at forging “no‐regret” forest‐based climate change mitigation strategies. Based on the consideration of forest growth dynamics and the opportunity carbon cost associated with wood use, we suggest that, instead of taking (hypothetical) wood‐for‐fossil substitution as starting point in assessments of carbon implications of wood products and services, analyses should take the potential and desired carbon sequestration of forests as starting point and quantify sustainable yield potentials compatible with those carbon sequestration potentials. Such an approach explicitly addresses the possible benefits provided by forests as carbon sinks, brings research on the permanence and vulnerability of C‐stocks in forests, of substitution effects, as well as explorations of demand‐side strategies to the forefront of research and, in particular, aligns better with the urgency to find viable climate solutions.
The Influence of Theme-Based Language Teaching toward Indonesian Students’ Reading Comprehension

Reading is one of the essential skills in English language learning. In the Indonesian context, reading has been one of the essential subjects to be taught among schools and universities since English is one of the compulsory requirements for graduation. Theme-based Language Teaching (TBLT) is a prominent approach used by the teacher and the students to understand the reading. This study aimed at examining the influence of TBLT on the students' reading comprehension. This research used quantitative research with an experimental study. The study selected participants from Madrasah Aliyah (MA) NW Narmada, Indonesia. The study recruited 20 students taken from the second grade of MA NW Narmada. Pre- and post-proficiency tests were used to assemble data of the study. To analyze data, this study employed a T-test to determine the significant result. The result indicated that the pretest score achieved 71.12 and the posttest value reached 89.05. It performed that different values occur on the students' reading comprehension after the TBLT implementation. The significant value (p) also indicated that the output reaches 0.00 < 0.05, which represented the result is under the significant level (0.05). It means that there is a significant influence of the TBLT method on the students' reading comprehension. In other words, TBLT method contributes positive in+uence on the students' English reading comprehension.
Modal damping for torsionally coupled buildings on elastic foundation

A method to determine the approximate normal modes and the modal damping for torsionally coupled buildings on an elastic foundation is presented. The modal damping is determined by an iterative procedure which matches the approximate normal mode solution with the rigorous solution. The response quantity to be matched is selected in a consistent and logical manner. The normal modes and the damping ratios thus found are then used to determine the seismic response of the interaction system by the response spectrum technique.
Specialised Robotic Hand Designing and Object Grasping Simulation

New Trend in the Specialised Robotic Hand Designing is Accelerated by Requirements on both Flexible and Intelligent Automated Manipulation with Objects of Different Types. Increasingly more Sophisticated Simulation Tools for Robotic Hand 3D Model Testing are Developed by Research Teams on the Whole World. the Article Presents the Methodology of the Robotic Hand Simulation Model Creation with the Goal to Test its Ability to Grasp the Object.
Awareness and Use of Current Sports Injury Prevention Programs among Physiotherapists Worldwide

Purpose. Physiotherapists are trained to prevent, assess, and rehabilitate all kinds of injuries including sports injury. The goal of the physical therapist should be making sure that the athlete is in optimal shape to perform, with a minimal risk for developing an injury. This study aims to assess the physiotherapists’ awareness, implementation, and views of sports injury prevention programs (IPPs) from an international perspective. Materials and methods. A self-administered questionnaire was developed and distributed to physiotherapists worldwide through World Physiotherapy member organizations. The study targeted physiotherapists at an international level. The study included 484 participants, of whom 44.4% were male and 55.6% were female physiotherapists. Results. A total of 287 (59.3%) of the participants were aware of the current sports IPPs, 177 (36.6%) were implementing sports IPPs in their current practice. Participants who implemented the sports IPPs reported a positive opinion about the program efficacy, with a score of 7.3 ± 2.11 out of 10. Conclusions. Globally, physiotherapists have average awareness and low implementation levels of IPPs. Physiotherapists showed a positive score regarding the effectiveness of IPPS, especially the KIPP and the iSPRINT.
The Role of Neutrophil Lymphocyte Ratio as a Major Adverse Cardiac Events Predictor and Its Correlation with Coronary Severity in Acute Coronary Syndrome Patients with Chronic Kidney Disease: A Case Control and Cross-Sectional Study

BACKGROUND: Acute coronary syndrome (ACS) patients with chronic kidney disease (CKD) are known to have a higher risk of mortality compared to ACS patients without CKD. In ACS patients with CKD, chronic inflammation plays an important role in morphological and functional changes in endothelial cells, resulting in atherosclerosis acceleration associated with coronary severity that leads to an increase in major adverse cardiac events (MACE). AIM: Therefore, this study aims to determine the role of neutrophil lymphocyte ratio (NLR) as a predictor of MACE and its correlation with coronary severity in ACS patients with CKD. METHODS: The study was conducted at National General Hospital Cipto Mangunkusumo Jakarta, Indonesia in October to November 2019. We used quota sampling with two designs study. First, a nested case control study was conducted with a total of 59 ACS patients with CKD: 31 subjects who had experienced MACE as a case group and 28 subjects who had not experienced MACE as a control group. Second, a correlative study with a cross-sectional approach was undertaken. RESULTS: There was no significant difference or relationship between NLR and MACE (p > 0.05; OR = 2.16 [95% CI = 0.63–7.51]), also no correlation between NLR and coronary severity degree assessed using the Gensini score (r = 0.10; p = 0.474). CONCLUSION: NLR can not predict MACE in ACS patients with CKD nor be employed interchangeably with the Gensini score in assessing coronary severity in ACS patients with CKD.
Evaluation the Immune Response of Salmonella Vaccines in Duck

The infection by Salmonella Typhimurium (ST) is one of the most prevalent causative agents of lethal diseases in ducklings and represents a potential public health problem. Avoidance the duck Salmonellosis is very mandatory. So, vaccination is considered an acceptable solution to the public objections of drug residues and provides an unexpected, serious safety and it seems to be a possible practical option. For this purpose, six hundred and forty serongative ducklings were clustered into four replicates which assigned to G1 (vaccinated by live attenuated ST vaccine at one day old age), G2 (vaccinated by inactivated ST vaccine at 15 days old age) and G3 (primed by live attenuated ST vaccine and boosted by inactivated ST vaccine after 21days). Control unvaccinated group still without any treatment (G4). All vaccinated and control groups were challenged weekly with by virulent S. Typimurium strain with a dose 0.1ml of 10 8 CFU/ml intramuscularly till 16 weeks post vaccination ( WPV ) and 13 weeks post boosting ( WPB ). The calculation of re-isolation% was done. From results, it was observed the G1 reached to satisfactory protection after 2 WPV despite, G2 reach the maximum protection at 4 (WPC). Concerning the re-isolation%, it was noticed the higher re-isolation% in G1 was detected at 5WPC in contrast G2 which still having low re-isolation% till 14 WPC. The G3 was considered the best because can protect duckling from infection during the first 3WPC and very low re-isolation% till 13 WPB. It was concluded that, live attenuated ST vaccine is very important at one day old age to avoid morbidities and mortalities till 3 weeks old age. The boosting by inactivated ST vaccine after brimming by live attenuated was potentiated immune response and reduced bacterial shedding. It was recommended for subsequent booster dose by inactivated ST vaccine before egg lying to confer satisfactory maternal antibodies level for ducklings.
Prevalence of nosocomial infections in Covid-19 patients admitted to the intensive care unit of Imam Khomeini complex hospital in Tehran

Background and Objectives: Nosocomial infections (NIs) are an important cause of mortality and morbidity in intensive care units (ICU). Pneumonia is the most common serious manifestation of infection in Covid-19 patients. The aim of this study was to investigate the prevalence of pneumonia in Covid-19 patients admitted to the ICU. Materials and Methods: In this cross-sectional study, 1240 Covid-19 patients admitted for more than 48 h in the ICUs of Imam Khomeini Complex Hospital (IKCH) in Tehran for seven months in 2020 were included in the study with initial diagnosis of Covid-19 (PCR test and chest imaging). Data were collected regarding severity of the illness, primary reason for ICU admission, presence of risk factors, presence of infection, length of ICU and hospital stay, microbial type and antibiotic resistance. In this study, the pattern of antibiotic resistance was determined using the disk difusion method. Results: In this study, 289 (23.3%) out of 1320 patients experienced NIs. 221 (76.4%) out of 289 patients had underlying diseases and the most common of which were hypertension, diabetes and heart disease, respectively. 163 patients (56.4%) were RT-PCR COVID-19 positive and 200 patients (69%) died. The majority of patients with NIs (71%) were over 55 years old. The most common type of nosocomial infection (66%) was ventilator-associated pneumonia (VAE). The most common microorganisms that cause pneumonia were Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa, respectively. Conclusion: Pneumonia infection is high in Covid-19 patients admitted to the ICU, it needs to be planned with the diagnosis and measures related to the control and prevention of this infection.
COVID-19 and RAAS inhibitors: is there a final conclusion?

Coronavirus disease 2019 (COVID-19), the first pandemic caused by a human infecting coronavirus, has drawn global attention from the first time it appeared in Wuhan city of China in late December 2019. Detection of the responsible viral pathogen, named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO, and its possible pathogenesis lead to the forming of many hypotheses about the factors that may affect the patients’ outcome. One of the SARS-CoV-2 infection concerns was the potential role of angiotensin-converting enzyme (ACE) inhibitors and angiotensin-receptor blockers (ARBs) in COVID-19 patients’ morbidity and mortality. Studies demonstrated that because SARS-CoV-2 uses human ACE2 cell receptors as an entry receptor to invade the cells, there might be an association between antihypertensive drugs such as RAAS inhibitors (specifically ACEIs and ARBs) and the COVID-19 disease. Data are scarce and conflicting regarding ACEI or ARB consumption and how it influences disease outcomes, and a single conclusion has not been reached yet. According to the literature review in our article, the most evidentially supported theory about the use of RAAS inhibitors in COVID-19 is that these medications, including ACEI/ARB, are not associated with the increased risk of infection, disease severity, and patient prognosis. However, further studies are needed to support the hypothesis.
Contact between a centrally loaded plate and a rigid or elastic base, with application to pivoted pad bearings

Abstract Coker and Filon first introduced the idea that the contact area between a pad and a support may decrease under the application of a load, the process subsequently called a ‘receding contact’ by Keer et al. Filon analysed the contact between two matching slabs under a line load, and showed that, for the two to remain in contact, the mid-plane contact stresses must change from compression to tension beyond a distance from the line load of approximately 1.35 times the slab thickness. Filon argued that, since the tensile stresses are small, in a real contact the two slabs will separate at approximately these locations. A photoelastic study by Frocht of the stress pattern when a line load acts through a pad resting on an elastic base supported Filon's prediction. It is shown by a three-dimensional finite element (FE) solution for the axisymmetric load problem that the radius of separation on a rigid base is significantly smaller than that calculated using the Filon assumption. When the pad is supported on an elastic base of the same material, the same is true, though both radii are significantly larger than with the rigid base. The results are applicable to several practical problems. For a rigid base, examples are two slabs pressed together by circular dies, and the initial geometry in resistance spot welding. The case of an elastic base is of wide application in civil engineering, when a pillar is supported on a bearing plate on an elastic base. In tribology it may explain the start-up under load of a centrally pivoted thrust pad bearing, which otherwise must rely on surface tension to retain a residual oil film.
A ground motion prediction equation for JMA instrumental seismic intensity for shallow crustal earthquakes in active tectonic regimes

The JMA (Japan Meteorological Agency) seismic intensity scale has been used in Japan as a measure of earthquake ground shaking effects since 1949. It has traditionally been assessed after an earthquake based on the judgment of JMA officials. In 1996 the scale was revised as an instrumental seismic intensity measure (IJMA) that could be used to rapidly assess the expected damage after an earthquake without having to conduct a survey. Since its revision, Japanese researchers have developed several ground motion prediction equations (GMPEs) for IJMA using Japanese ground motion data. In this paper, we develop a new empirical GMPE for IJMA based on the strong motion database and functional forms used to develop similar GMPEs for peak response parameters as part of the PEER (Pacific Earthquake Engineering Research Center) Next Generation Attenuation (NGA) project. We consider this relationship to be valid for shallow crustal earthquakes in active tectonic regimes for moment magnitudes (M) ranging from 5.0 up to 7.5–8.5 (depending on fault mechanism) and rupture distances ranging from 0 to 200 km. A comparison of this GMPE with relationships developed by Japanese researchers for crustal and shallow subduction earthquakes shows relatively good agreement among all of the relationships at M 7.0 but relatively poor agreement at small magnitudes. Our GMPE predicts the highest intensities at small magnitudes, which together with research on other ground motion parameters, indicates that it provides conservative or upwardly biased estimates of IJMA for M<5.5. Copyright © 2010 John Wiley & Sons, Ltd.
Secondary lobe level control in antenna arrays

Antenna arrays are usually employed to obtain a specified radiation pattern, either omnidirecional in a plane or directional with narrow main lobe and low secondary lobe level. This article presents a solution that makes it possible to obtain, for a specified plane, secondary lobe levels smaller than those obtained with the conventional configuration in antenna arrays, showing that it is possible to have one extra degree of freedom in the geometric configuration of an antenna array, which allows us to control, in a more effective way, the array characteristic parameters. These results are of general use for several array types, including smart antenna arrays where it is important to know the mentioned characteristics. Measurements made with antenna arrays in the VHF band showed a behaviour compatible with the presented conclusions.
Familial and Socio-Cultural Factors Contributing to the Rising Trend of Drug Addiction in Youth in Pakistan

Aim: To know about the major familial and socio-cultural factors contributing to the rising trend of drugs and substance use in youth in twin cities i.e. Rawalpindi and Islamabad. Methods: The study was conducted in July-September 2021 in Twin Cities i.e. Rawalpindi and Islamabad. Data was collected from 150 young drug addicts (between the age group of 15 to 40 years). Snow ball and purposive sampling techniques were used to collect the data. A pre devised interview schedule having close and open ended questions was used. Frequency analysis method was used to represent the basic demographic data whereas a 4-points Likert Scale was used to know the extent of factors contributing to the rising trend of drug addiction in youth. Results: Results showed that slightly more than one third of the respondents (34%) were quite young (21-25 years). Peer group/ bad company was found responsible factor to great extent (71%), To increase sexual pleasure was also found responsible (45.8%), Lack of knowledge about harms of drugs was found responsible to great extent (49.3%). Another factor i.e. curiosity to use / taste drugs was also found responsible to great extent (77.3%). Conclusion: Problem of drug addiction in youth in Pakistan is rising at a worrying trend. Numerous familial as well socio-cultural factors in the etiology of drug abuse and addiction have been found responsible. Keywords: Drug, Substance, Addiction, youth, familial, social-cultural, factors, Pakistan,
Postoperative Pulmonary Complications After Cardiac Surgery: The VENICE International Cohort Study.

OBJECTIVE Postoperative pulmonary complications (PPC) remain a main issue after cardiac surgery. The objective was to report the incidence and identify risk factors of PPC after cardiac surgery. DESIGN An international multicenter prospective study (42 international centers in 9 countries). PARTICIPANTS A total of 707 adult patients who underwent cardiac surgery under cardiopulmonary bypass. INTERVENTIONS None MEASUREMENTS AND MAIN RESULTS: During a study period of 2 weeks, the investigators included all patients in their respective centers and screened for PPCs. PPC was defined as the occurrence of at least 1 pulmonary complication among the following: atelectasis, pleural effusion, respiratory failure, respiratory infection, pneumothorax, bronchospasm, or aspiration pneumonitis. Among 676 analyzed patients, 373 patients presented with a PPC (55%). The presence of PPC was significantly associated with a longer intensive care length of stay and hospital length of stay. One hundred ninety (64%) patients were not intraoperatively ventilated during cardiopulmonary bypass. Ventilation settings were similar regarding tidal volume, respiratory rate, inspired oxygen. In the regression model, age, the Euroscore II, chronic obstructive pulmonary disease, preoxygenation modality, intraoperative positive end-expiratory pressure, the absence of pre- cardiopulmonary bypass ventilation, the absence of lung recruitment, and the neuromuscular blockade were associated with PPC occurrence. CONCLUSION Both individual risk factors and ventilatory settings were shown to explain the high level of PPCs. These findings require further investigations to assess a bundle strategy for optimal ventilation strategy to decrease PPC incidence.