Miracle-dz/UniFAR-FTUs huggingface dataset:
Community gardens as local learning environments in social housing contexts: participant perceptions of enhanced wellbeing and community connection

ABSTRACT Urban community gardens provide learning environments for diverse groups, including those who may be experiencing health and social inequalities such as residents in social housing communities. Learning to grow fresh food in safe social spaces provides individuals with opportunities to increase awareness of their personal wellbeing and community life. This paper reports on the findings of a research study that explored broader impacts of a community gardening programme on 42 adult residents living in social housing estates in Sydney, Australia. The mixed-methods study design captured participants’ self-perceived benefits of community gardening across six new sites. A final sample of 23 participants across the sites completed both the Sense of Community Index 2 and the Personal Wellbeing Index questionnaires at pre- and post-test (following six to seven months of being involved in the programme). Focus groups involved 42 participants from all six sites. Perceived benefits included enhanced awareness of their overall health and wellbeing, new interest in growing fresh food, enjoyment of shared produce and recipes, feelings of happiness, frequent socialisation and community connectedness. The findings highlight the impactful role of community gardens as effective local learning environments that promote psychological wellbeing and community connection in underserved communities. We conclude by reinforcing the need for sustainable community gardens for addressing social inequality and promoting multiple psychosocial benefits.
A pipeline for effectively developing highly polymorphic simple sequence repeats markers based on multi‐sample genomic data

Abstract Simple sequence repeats (SSRs) are widely used genetic markers in ecology, evolution, and conservation even in the genomics era, while a general limitation to their application is the difficulty of developing polymorphic SSR markers. Next‐generation sequencing (NGS) offers the opportunity for the rapid development of SSRs; however, previous studies developing SSRs using genomic data from only one individual need redundant experiments to test the polymorphisms of SSRs. In this study, we designed a pipeline for the rapid development of polymorphic SSR markers from multi‐sample genomic data. We used bioinformatic software to genotype multiple individuals using resequencing data, detected highly polymorphic SSRs prior to experimental validation, significantly improved the efficiency and reduced the experimental effort. The pipeline was successfully applied to a globally threatened species, the brown eared‐pheasant (Crossoptilon mantchuricum), which showed very low genomic diversity. The 20 newly developed SSR markers were highly polymorphic, the average number of alleles was much higher than the genomic average. We also evaluated the effect of the number of individuals and sequencing depth on the SSR mining results, and we found that 10 individuals and ~10X sequencing data were enough to obtain a sufficient number of polymorphic SSRs, even for species with low genetic diversity. Furthermore, the genome assembly of NGS data from the optimal number of individuals and sequencing depth can be used as an alternative reference genome if a high‐quality genome is not available. Our pipeline provided a paradigm for the application of NGS technology to mining and developing molecular markers for ecological and evolutionary studies.
Identification and Functional Analysis of Glutathione S-Transferases from Sitophilus zeamais in Olfactory Organ

Simple Summary Sitophilus zeamais is a worldwide pest that destroys many grain products, causing a loss of cereal quality and quantity resulting from its metabolites and behavior. Glutathione S-transferases (GSTs), as a group of odorant-degrading enzymes (ODEs), play an important role in degrading xenobiotic odorant molecules in insect olfactory sensing systems. However, there have been few reports about the function of the GST genes of S. zeamais in the odorant-degrading process. In this study, we characterized 13 full-length genes encoding GST sequences from S. zeamais and analyzed the expression pattern in different tissues of SzeaGSTd1. In addition, we investigated the ability of recombinant SzeaGSTd1 to degrade the volatile molecules of the host, and the data indicated that the content of capryl alcohol significantly decreased in the system. In summary, we believe SzeaGSTd1 plays a key role in the olfactory sensing system of S. zeamais. Abstract Odorant-degrading enzymes (ODEs) play an important role in rapidly degrading and inactivating odorant molecules that have completed information transmission, as well as in maintaining the stability and sensitivity of insect olfactory sensing systems. Glutathione S-transferases (GSTs), as a group of ODEs, supposedly bear the ability to catalyze the conjugation of glutathione (GSH) and xenobiotic odorant molecules in the degrading process. However, there are few reports regarding the role of the GST genes of Sitophilus zeamais in the degrading process. Thus, we characterized 13 full-length genes encoding GST sequences from S. zeamais, of which only SzeaGSTd1 contained a high abundance in the antennae. Ligand-binding assays implied that SzeaGSTd1 was able to catalyze the conjugation of GSH with 2, 4-dinitrochlorobenzene (CDNB). We investigated whether recombinant SzeaGSTd1 bears the ability to degrade the volatile molecules of the host; among the host volatiles, and found capryl alcohol to be a suitable substrate for SzeaGSTd1. These results strongly suggest that SzeaGSTd1 probably plays a role in auxiliary host location by degrading the host volatiles of capryl alcohol and exhibits a potential biological function in the olfactory sensing system of S. zeamais. Knowledge of the potential functions of SzeaGSTd1 will provide new ideas for biological control strategies for S. zeamais.
The Steps Taken by The Prophet Muhammad In Building Da‘wah And Islamic Civilization In Medina According to The Book of Ar-Rohiqul Makhtum

This study aims to reveal the real steps taken by the Prophet Muhammad in Medina, which enable Muslims to develop a strong and influential civilization in the World. This is library research. The author used the book Ar-Rohiqul Makhtum by Al-Mubarakfuri as the main reference. The author reached the conclusion that the Prophet Muhammad took several strategic steps in his da'wah in Medina, including 1) Building a Mosque, 2) Brothering Muslims from the Muhajirin and Ansar, 3) Making a peace treaty with all of the Medina Citizens. The author agrees with Nabil As-Samaluti (2019) and Imam Amrusi Jailani (2016) that the concept of the State of Medina is a concept of peace for the entire citizens. This article also supports Komaruddin (2011), who stated that the Islamic State was not founded by coercion and violence.
Knowledge and practice of weaning among mothers attending the paediatric outpatient clinic in a tertiary hospital in southern Nigeria

Background : Adequate weaning confers both short and long-term benefits on a child’s health and well-being. Weaning practice is determined by a mother’s knowledge amongst other factors. Methods : This was a cross-sectional questionnaire-based study carried out from 1 st of June - 31 st of May 2021. Assessments were graded as good, fair and poor knowledge or practice. Data was analysed using SPSS version 23. Fishers’ Exact test was used to test for statistical significance, with P value set at <0.05. Results : Most mothers were aged 30-35years 67(38.7%), married 171(98.8%), resided in the urban area 146(84.4%), were traders/businesswomen 73(42.2%), had tertiary education 136(78.6%) and had a parity of 2, 61(35.3%). Majority 110(63.6%) had heard of the word ‘weaning’ but only 72(43.9%) could correctly define it. Majority 145(83.8%) had good knowledge while 5(2.9%) had poor knowledge. Majority 106(61.3%) had good practice while 2(1.1%) had poor practice. Majority 121(69.9%) of mothers who weaned their babies before 6 months did so because they felt the breast milk was not enough 21(42.6%). Others felt they were stressed 7(14.9%) and because of resumption at work 7(14.9%). Most mothers who stopped breastfeeding before 2 years of age did so mainly because the babies stopped on their own 21(25.6%), ate more of complimentary foods 20(24.4%) and because of work pressure 9(11.1%). There was significant association between the mother’s occupation (P value=0.003), and level of education (P value < 0.001) with the level of weaning knowledge. There was a significant association between the level of practice of weaning and male sex (P value=0.016). Conclusion : There was good knowledge and good practice of weaning among mothers attending the paediatric outpatient clinic. There is however still room for improvement of weaning practices by mothers by further education on weaning as well as provision of support by community support groups.
Effect of Graphene vs. Reduced Graphene Oxide in Gold Nanoparticles for Optical Biosensors—A Comparative Study

Aiming to develop a nanoparticle-based optical biosensor using gold nanoparticles (AuNPs) synthesized using green methods and supported by carbon-based nanomaterials, we studied the role of carbon derivatives in promoting AuNPs localized surface plasmon resonance (LSPR), as well as their morphology, dispersion, and stability. Carbon derivatives are expected to work as immobilization platforms for AuNPs, improving their analytical performance. Gold nanoparticles (AuNPs) were prepared using an eco-friendly approach in a single step by reduction of HAuCl4·3H2O using phytochemicals (from tea) which act as both reducing and capping agents. UV–Vis spectroscopy, transmission electron microscopy (TEM), zeta potential (ζ-potential), and X-ray photoelectron spectroscopy (XPS) were used to characterize the AuNPs and nanocomposites. The addition of reduced graphene oxide (rGO) resulted in greater dispersion of AuNPs on the rGO surface compared with carbon-based nanomaterials used as a support. Differences in morphology due to the nature of the carbon support were observed and are discussed here. AuNPs/rGO seem to be the most promising candidates for the development of LSPR biosensors among the three composites we studied (AuNPs/G, AuNPs/GO, and AuNPs/rGO). Simulations based on the Mie scattering theory have been used to outline the effect of the phytochemicals on LSPR, showing that when the presence of the residuals is limited to the formation of a thin capping layer, the quality of the plasmonic resonance is not affected. A further discussion of the application framework is presented.
Vulnerability and Risk Assessment to Climate Change in Sagar Island, India

Inhabitants of low-lying islands face increased threats due to climate change as a result of their higher exposure and lesser adaptive capacity. Sagar Island, the largest inhabited estuarine island of Sundarbans, is experiencing severe coastal erosion, frequent cyclones, flooding, storm surges, and breaching of embankments, resulting in land, livelihood, and property loss, and the displacement of people at a huge scale. The present study assessed climate change-induced vulnerability and risk for Sagar Island, India, using an integrated geostatistical and geoinformatics-based approach. Based on the IPCC AR5 framework, the proportion of variance of 26 exposure, hazard, sensitivity, and adaptive capacity parameters was measured and analyzed. The results showed that 19.5% of mouzas (administrative units of the island), with 15.33% of the population at the southern part of the island, i.e., Sibpur–Dhablat, Bankimnagar–Sumatinagar, and Beguakhali–Mahismari, are at high risk (0.70–0.80). It has been concluded that the island has undergone tremendous land system transformations and changes in climatic patterns. Therefore, there is a need to formulate comprehensive adaptation strategies at the policy- and decision-making levels to help the communities of this island deal with the adverse impacts of climate change. The findings of this study will help adaptation strategies based on site-specific information and sustainable management for the marginalized populations living in similar islands worldwide.
Clinical relevance of serum-derived exosomal messenger RNA sequencing in patients with non-Hodgkin lymphoma

Background: The clinical utility of mRNA cargo in exosomes is unclear, although exosomes have potential as non-invasive biomarkers. This study aimed to investigate the feasibility of exosomal mRNA sequencing for monitoring disease status and predicting outcomes in non-Hodgkin lymphoma (NHL) patients. Methods: Exosomes were isolated from archived serum samples of 33 patients with NHL who were registered into our prospective cohort: diffuse large B-cell lymphoma (DLBCL, n = 17), intravascular B-cell lymphoma (IVL, n = 1), primary mediastinal large B-cell lymphoma (PMBL, n = 4), follicular lymphoma (FL, n = 3), mantle cell lymphoma (MCL, n = 3), and extranodal NK/T-cell lymphoma (ENKTL, n = 5). Exosomal mRNA sequencing was performed, and its concordance with clinical course was analyzed and compared with those of circulating tumor DNA (ctDNA) mutations. Results: Exosomal mRNA sequencing was performed successfully in 26 cases (79%, 26/33), whereas the remaining seven cases were not completed due to their small amount of RNA. The exosomal mRNA sequencing of DLBCL showed gene expression profiles consistent with activated B-cell-like and germinal center type. The longitudinal assessment of exosomal mRNA sequencing results in accordance with the clinical course showed that the post-treatment changes of exosomal mRNA expression were more consistent with treatment outcome than were those of ctDNA mutations. In particular, the exosomal mRNA expression of genes such as BCL2 and BCL6 was increased at the time of disease progression in DLBCL and FL patients. Conclusions: This study demonstrated the feasibility of exosomal mRNA expression profiles as a biomarker for NHL patients. Our results might provide the rationale for studies to explore the potential of exosomal mRNA as a biomarker in NHL patients.
Magnetic Resonance Imaging in (Near-)Term Infants with Hypoxic-Ischemic Encephalopathy

Hypoxic-ischemic encephalopathy (HIE) is a major cause of neurological sequelae in (near-)term newborns. Despite the use of therapeutic hypothermia, a significant number of newborns still experience impaired neurodevelopment. Neuroimaging is the standard of care in infants with HIE to determine the timing and nature of the injury, guide further treatment decisions, and predict neurodevelopmental outcomes. Cranial ultrasonography is a helpful noninvasive tool to assess the brain before initiation of hypothermia to look for abnormalities suggestive of HIE mimics or antenatal onset of injury. Magnetic resonance imaging (MRI) which includes diffusion-weighted imaging has, however, become the gold standard to assess brain injury in infants with HIE, and has an excellent prognostic utility. Magnetic resonance spectroscopy provides complementary metabolic information and has also been shown to be a reliable prognostic biomarker. Advanced imaging modalities, including diffusion tensor imaging and arterial spin labeling, are increasingly being used to gain further information about the etiology and prognosis of brain injury. Over the past decades, tremendous progress has been made in the field of neonatal neuroimaging. In this review, the main brain injury patterns of infants with HIE, the application of conventional and advanced MRI techniques in these newborns, and HIE mimics, will be described.
GPS Results from Long Time Monitoring of Geodynamic Processes in South-Western Bulgaria

Monitoring of geodynamic processes by modern GNSS techniques in the area of Sofia and South-Western Bulgaria has continued for 25 years. To study the modern crustal movements in the area, Global Positioning System (GPS) data acquired between 1996 and 2021 are analyzed to obtain the velocity field for South-Western Bulgaria. For a time period of almost 25 years, the monitoring has covered 28 stations. They have been measured in different years and in a number of campaigns. Despite the difference in the measurements, the obtained results are quite homogeneous in the different localities of the studied area and show clear uniform tendencies. All velocities are in the southern direction. They are in the range of 1.5 mm/year to slightly over 3 mm/year, almost reaching 4 mm/year. The velocities of the stations tend to increase from north (stations around Sofia), passing through an intermediate locality (between Sofia and Kyustendil–Pazardhik), clearly increasing in the southernmost part of the country (around Gotse Delchev). This velocity field motivates N–S expressed extension with increasing rates from North to South. The difference in the velocity rates tends to change along geologically suggested active fault zones. The obtained results generally confirm previously data, but with much better accuracy and details at the local level. This way, both the repeated measurements and extension of the geodynamic network prove to be a powerful tool for a better understanding of present-day geodynamics.
Microstructure and Mechanical Properties of Dissimilar Friction Stir Welded Joint AA7020/AA5083 with Different Joining Parameters

The present paper aims to analyze the influence of process parameters (tool traverse speed and tool rotational speed) on the macrostructure, microhardness, and mechanical properties of dissimilar friction stir welded (FSW) butt joints. Nine combinations of FSW parameters welded joints of aluminum alloys 7020-T651 and 5083-H111 were characterized. Plates in 5 mm thickness were welded using the FSW method as dissimilar joints with three values of tool rotation parameters (400, 800, and 1200 rpm) and three welding speeds (100, 200, 300 mm/min). The macroscopic observations revealed various shapes of the stir zone and defects resulting from excess and insufficient heat input. Microfractographic analysis and tensile test results showed that the samples made with the FSW parameters of 800 rpm and 200 mm/min had the best strength properties: UTS = 303 MPa, YS = 157 MPa, and A = 11.6 %. Moreover, for all welds at welding speed 100 mm/min, the joint efficiency reached 95%.
A Composting Bedding System for Animals as a Contribution to the Circular Economy

By-products from forestry, agriculture and nature areas are used in compost bedded-pack housing (CBP) systems for animals. In this communication, we discuss the application of a CBP system to animal farms and aspects related to the recycling and reuse of the materials in the context of a circular economy. This study is based on data from ongoing projects and literature. The following systems are discussed: (i) composting material applied to a specialized animal housing system; (ii) adding a horticultural component to the animal farm by reusing the compost, and (iii) a cooperative mixed cattle and crop farming system. The success of integrating a compost bedding component in the system depends largely on the skills of managing the composting process, the application of the material in the field, and the cost of acquiring the material. When materials are amply available, then a real contribution to the circular economy can be made. Cooperation between farmers in the utilization of by-products is another route to a more circular economy. Moreover, the analyzed systems can be seen as a Greenhouse Gases (GHG) mitigation practice because they store carbon in the soil and improve soil quality.
Procedural Outcomes in Patients Treated with Percutaneous Coronary Interventions within Chronic Total Occlusions Stratified by Gender

It has been demonstrated that gender differences are related to different procedural and long-term clinical outcomes among a general patient population treated using percutaneous coronary interventions (PCI). The objective of our analysis was to conduct assessment regarding the relationship between gender and procedural outcomes in patients treated for PCI regarding chronic total occlusions (CTO), based on a large, real-life registry. Data used to conduct the following analysis was derived from the national registry of percutaneous coronary interventions (ORPKI), upheld in co-operation with the Association of Cardiovascular Interventions (AISN) of the Polish Cardiac Society. The study involved data procured from the registry within the period from January 2014 to December 2020. All subsequent CTO procedures recorded in the registry during that period were included in the analysis. We assessed the correlation between gender and the overall rate of periprocedural complications, procedure-related mortality, and success evaluated as TIMI flow grade 3 after the procedure by univariate and multivariable modeling. At the time of conducting our investigation, there were 162 existing and active CathLabs, at which 747,033 PCI procedures were carried out during the observational period. Of those, 14,903 (1.99%) were CTO-PCI procedures, and 3726 were women (25%). The percentage share between genders did not experience any significant changes during the consecutive years observed in the current analysis. Overall periprocedural complication rate was greater among women than men (3.45% vs. 2.31%, p = 0.02). A comparable relationship was noted for procedural mortality (0.7% vs. 0.2%, p = 0.006), while procedural success occurred more often in the case of women (69.3% vs. 65.2%, p < 0.001). Women were found to be more frequently affected by periprocedural complications (OR = 1.553; 95%CI: 1.212–1.99, p < 0.001) as well as procedural success (OR = 1.294; 95%CI: 1.151–1.454, p < 0.001), evaluated using multivariable models. Based on the current analysis performed on all-comer patients treated using PCI in CTO, women are affected by more frequent procedural complication occurrence as well as greater procedural success compared to men.
Habitat occupancy of sloth bear Melursus ursinus in Chitwan National Park, Nepal

Abstract Mammals have experienced a massive decline in their populations and geographic ranges worldwide. The sloth bear, Melursus ursinus (Shaw, 1791), is one of many species facing conservation threats. Despite being endangered in Nepal, decades of inattention to the situation have hindered their conservation and management. We assessed the distribution and patterns of habitat use by sloth bears in Chitwan National Park (CNP), Nepal. We conducted sign surveys from March to June, 2020, in 4 × 4 km grids (n = 45). We collected detection/non‐detection data along a 4‐km trail that was divided into 20 continuous segments of 200 m each. We obtained environmental, ecological, and anthropogenic covariates to understand determinants of sloth bear habitat occupancy. The data were analyzed using the single‐species single‐season occupancy method, with a spatially correlated detection. Using repeated observations, these models accounted for the imperfect detectability of the species to provide robust estimates of habitat occupancy. The model‐averaged occupancy estimate for the sloth bear was 69% and the detection probability was 0.25. The probability of habitat occupancy by sloth bears increased with the presence of termites and fruits and in rugged, dry, open, undisturbed habitats. Our results indicate that the sloth bear is elusive, functionally unique, and widespread in CNP. Future conservation interventions and action plans aimed at sloth bear management must adequately consider their habitat requirements.