Miracle-dz/UniFAR-FTUs huggingface dataset:
Sildenafil enhances cisplatin-induced apoptosis in human breast adenocarcinoma cells

Introduction: Cyclic nucleotide phosphodiesterase (PDE) enzymes are a large superfamily of enzymes that catalyze the conversion reaction of cyclic adenosine monophosphate (AMP) and cyclic guanosine monophosphate (GMP) to AMP and GMP, respectively. In some cancer cells, PDE-5 has been shown to be overexpressed in multiple human carcinomas. It seems that the inhibition of PDE-5 may has anticancer effects. Cisplatin is one of the prevalent chemo-agents to treat solid tumors. However, its clinical usefulness is hindered by dose-limiting toxicities, especially on the kidneys (nephrotoxicity) and ears (ototoxicity). In this study, the antitumor activity of the sildenafil as a PDE-5 inhibitor alone and in combination with cisplatin on human mammary adenocarcinomas and MCF-7 and MDA-MB-468 was assessed. Materials and Methods: Sildenafil as PDE type 5 (PDE5) inhibitor is the drugs that we combined with the cisplatin (chemotherapeutic agent), in vitro. Human mammary adenocarcinomas and MCF-7 and MDA-MB-468 cell lines were cultured in standard conditions. At time point, following 24 h and 48 h incubation, the cell lines were treated by cisplatin in the presence/absence of sildenafil. Cell viability, apoptosis, and reactive oxygen species (ROS) were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, real-time polymerase chain reaction, and Western blot; and fluorimetric methods, respectively. Statistical analysis was performed using SPSS software SPSS (SPSS Inc., Chicago, IL, USA). Results: In MCF-7 cell line, following 24 h incubation, combinations of sildenafil with cisplatin (P < 0.001) showed decreased cell viability when compared to sildenafil and cisplatin alone. Moreover in MDA-MB-468 cell line, following 24 h incubation, data did not show any significant changes on cell viability when treated with cisplatin, in the presence or absence of sildenafil. However, following 48 h incubation, combinations of cisplatin with sildenafil (P < 0.001) were showed decreased cell viability when compared to cisplatin and sildenafil alone in both MCF-7 and MDA-MB-468 cell lines. Concerning the ROS production and apoptosis, data showed that both processes increase significantly in the presence of the sildenafil in comparison absent it. Conclusion: Our data showed that the combination of sildenafil with cisplatin can improve cell toxicity and anticancer effect of cisplatin. And also sildenafil as a PDE-5 inhibitor could be used as additive treatment in combination with cisplatin to make a reduction in cisplatin dosage and its side effects.
Pilot study for the potential application of a shortcut nitrification and denitrification process in landfill leachate treatment with MBR

An advanced nitrogen removal pilot study was performed in China's Xia Ping Landfill Leachate Treatment Plant to undertake shortcut nitrification and denitrification with the Membrane Bio-reactor (MBR) process. It was found that the MBR process used 25% less of the oxygen and 40% less of the external carbon sources, compared to the conventional nitrification and denitrification process. The key feature of the MBR process is that it provides an environment more favorable for ammonia oxidation bacterium (AOB) than for nitrite oxidation bacterium (NOB) through controlling loading, pH, temperature, dissolved oxygen concentration (DO), and NH 3 inhibition. Optimum operating condition was examined through continuous running of the pilot MBR, and it was found that a minimum HRT of 4.3 days and maximum ammonia loading of 0.6kg N-NH 4 + m 3 . d with pH 7-8.5, temperature 25-30°C, and DO at 2mg/L is favorable to AOB. Kinetic study was conducted to identify the characteristic of the microorganisms in the system. Measured K s and μA. max of MBR sludge was 19.65 mg NH 4 -N/L (Temperature 25 °C, pH 8.5) and 0.26 d -1 , respectively.
Computational determination of equilibrium geometry and dissociation energy of the water dimer

The equilibrium geometry and dissociation energy of the water dimer have been determined as accurately as technically possible. Various quantum chemical methods and high-quality basis sets have been applied—that is, at the level of a nearly complete basis—and both the intermolecular separation and the deformation of the donor and acceptor molecules have been optimized at the level of CCSD(T) theory (coupled-cluster theory with singles and doubles excitations plus a perturbation correction for connected triples). It is found at the CCSD(T) level that the monomer deformation in the dimer amounts to 86% of the deformation computed at the MP2 level (second-order Moller-Plesset perturbation theory) and that the core/valence electron correlation effects at the CCSD(T) level amount to 80% of the same effects at the MP2 level. The equilibrium O···O distance is determined as Re=291.2±0.5 pm and the equilibrium dissociation energy as De=21.0±0.2 kJ mol−1, with respect to dissociation into two isolated water molecules at equilibrium. Accounting for zero-point vibrational energy, the theoretical prediction for the dissociation energy becomes D0=13.8±0.4 kJ mol−1, a result which is open to direct experimental verification.
Customized BERT with Convolution Model : A New Heuristic Enabled Encoder for Twitter Sentiment Analysis

The Twitter messaging service has turned out to be a domain for news consumers and patrons to convey their sentiments. Capturing these emotions or sentiments in an accurate manner remains a major challenge for analysts. Moreover, the Twitter data include both spam and authentic contents that often affects accurate sentiment categorization. This paper introduces a new customized BERT (Bidirectional Encoder Representations from Transformers) based sentiment classification. The proposed work consists on pre-processing and tokenization step followed by a customized BERT based classification via optimization concept. Initially, the collected raw tweets are pre-processed via "stop word removal, stemming and blank space removal". Prevailing semantic words are acquired, from which the tokens (meaningful words) are extracted in the tokenization phase. Subsequently, these extracted tokens will be subjected to classification via optimized BERT, which weights and biases are optimally tuned by Standard Lion Algorithm (LA). In addition, the maximum sequence length of BERT encoder is updated with standard LA. Finally, the performance of the proposed work is compared over other state-of-the-art models with respect to different performance measures.
Quantification of Eugenol in Hydro-Distilled Clove Oil (Eugenia caryophyllus) and Its Marketed Products by Validated GC-MS Method

A gas chromatography/mass spectrometric (GC-MS) method was developed and validated for the analysis of eugenol in clove (Eugenia caryophyllus) in hydro-distilled oils and marketed formulations. Separation was done on HP5 MS column (5% phenyl polymethyl siloxane; 30 m × 0.25 mm i.d. and 0.25-μm film thickness), using the EI/CI MSD as the detector and helium at a flow rate of 1 mL.min−1 as the carrier gas. The method was validated as per the ICH guidelines for linearity, precision, accuracy, robustness, LOD, and LOQ. The eugenol in marketed formulations of clove oil analyzed and validated by this method ranged from 3% to 48%.
Role of Extensive Reading Habits in Students’ Acquisition of Composition Writing Skills in English in Kenya

Despite the important role reading proficiency plays in learners daily lives both within and outside school, there has been a lot of concern from educationist, researchers and media in Kenya who have reported a saddening trend on poor reading culture among many students in Kenya, This study was prompted by the observed poor reading and writing in English by secondary schools learners in Laikipia County in Kenya. The study therefore sought to establish whether there existed any relationship between extensive reading habits and composition writing amongst learners. The study was guided by Schmidt's (1975) Schema Theory. The descriptive survey research design was used for this study. Fifteen secondary schools in Laikipia County were sampled by proportional stratified sampling technique in five sub-counties across the county. Purposive sampling was used to sample 30 out of 80 (37.5%) English language teachers. Simple random sampling was used to sample 327 students out of about 2670 students (12.24%) in form 2 and 3 in the County. The research instruments used for data collection included: questionnaire for students, interview guides for teachers and composition writing test. The instruments were pilot tested to ensure validity and reliability in a co- educational secondary school in the neighbouring county that was not included in the study. The reliability of the questionnaire and interview guide was estimated using Cronbach’s alpha coefficient which was 0.871 which met the recommended threshold of 0.7 and above. Analysis of data was done using descriptive statistics such as frequency, tables and percentages. Analysis of Variance (ANOVA) was done at 95% confidence level (F(2) = 80.933, P = . 001) to establish whether there was statistically significance difference between composition writing mean scores of different categories of school using (SPSS) version 17. Qualitative data were organized according to the study themes and presented descriptively on the basis of the study objective. It was found that learners who engaged actively in extensive reading performed better in composition writing than those who did not. However, it was established that acute shortage of reading resources was a major hindrance to engagement in extensive reading in schools. The study recommended that schools should come up with strong extensive reading policy to promote independent reading so as to improve their writing skills. K e ywords : Extensive reading, Habits, Acquisition DOI: 10.7176/JEP/11-30-09 Publication date: October 31 st 2020
The depression anxiety and stress Scales-21: Bifactor statistical indices in support of the total and depression scores

This study explored several, latent factor models of the Depression Anxiety and Stress Scale? 21 (DASS? 21) using both a sample of clinically depressed patients and a Facebook sample from Serbia. The DASS? 21, the Beck Depression Inventory? II, and the State Trait Anxiety Inventory? Trait were administered to a sample of depressed individuals (N = 296; Mage = 52.21, SDage = 11.56). A Facebook sample (N = 376; Mage =29.12, SD = 8.96) completed the DASS? 21 only. A bifactor model with one general distress (G) and two specific factors (Depression and Anxiety) were supported. The three factors had high omega coefficients, whereas omega hierarchical for Depression and Anxiety were low. Based on all evidence from our study, external validation, factor determinacy, and replicability, we concluded that the Serbian version of the DASS? 21 assesses reliably general distress and anhedonia in both people with the clinical level of severity of distress and in general population. The Anxiety subscale can be safely used in clinical settings when one wishes to assess individuals presenting with a mixture of distress and anxious arousal. However, until further validation support is provided, we do not recommend use of this scale when the task is to estimate precisely anxious arousal only. The Stress subscale assesses general distress only. Low omega hierarchical coefficients of the Depression and Anxiety subscales could be addressed by re-selecting their items from the initial item pool, based on which the DASS? 21 was created, using those with substantial loadings on both the G and their respective group factors.
Does Consumer Demographics Effects Store Loyalty in Departmental Stores

In the fiercely competitive Indian retail sector, department stores are experiencing competitive pressures due to increased competition from discount stores, specialty stores, and aggressive e-commerce players. These stores are finding it difficult to hold their customers for a long time from switching to competing retailers and develop store loyalty for their success & growth. Given this backdrop, the present study aims to examine store loyalty across leading department stores and the impact of customer demographics (age, gender, marital and income on these departmental stores. The study was undertaken in the National Capital Region of Delhi and the primary data was collected from 287 respondents, who purchased regularly in one of the five leading department stores - Shoppers Stop, Lifestyle, Pantaloons, Globus, and Westside. Statistical analysis of the data was done with the help of SPSS 20 software. The key statistical tools applied were Independent sample t-test, one-way ANOVA and Post-hoc tests for data analysis. The results reveal significant differences in customer loyalty towards different department stores. Shoppers Stop emerged as the best department store in terms of store loyalty followed by Westside, Pantaloons, Globus and Lifestyle store. The study also reveals significant differences in-store loyalty between different age groups, male and female customers, as well as between different income groups.
THE INFLUENCE OF JOB EMBEDDEDNESS, LEADERSHIP AND MOTIVATION OF THE WORK ENGAGEMENT AND ITS IMPLICATIONS IN THE PERFORMANCE OF PT. BRI BANKS OF SIGLI

This research examines the effect of job embeddedness, leadership and motivation on work engagement and its implications for the performance of PT. BRI Bank Sigli Branch. The right research population is all employees of PT. BRI Bank Sigli Branch totaling 127 people. Sampling was done by census technique on all employees at PT. BRI Bank Sigli Branch. The research model was analyzed using Structural Equation Modeling (SEM) through the use of the Amos application. The results of this study prove that Job embeddedness influences work engagement, leadership also influences work engagement, work motivation influences work engagement, Job embeddedness influences organizational performance, Leadership influences organizational performance, Work motivation possessed by office employees affects organizational performance, Work engagement has an influence on improving organizational performance, work engagement mediates the influence of job embeddedness on organizational performance, work engagement mediates the influence of leadership on organizational performance, and work engagement mediates the effect of work motivation on organizational performance. The results of this study indicate that to ensure the performance of PT. BRI Bank Sigli Branch, focus must be given to increase work engagement through increased job embeddedness, leadership, and work motivation within the company. These findings contribute academically, especially in the renewal of the theory of causality and can be a reference for practical leaders, especially those in PT. BRI Bank Sigli Branch. The novelty of the results of this study lies in the combination of the previous causality research model, using new objects. The limitation of the study is the number of variables studied and the scope of the study. Keyword: Job Embeddedness, Leadership, Motivation, Work Engagement, Organizational Performance.
Electrically conductive aerogels composed of cellulose and carbon nanotubes

Aerogels composed of carbon nanotubes (CNTs) and cellulose were fabricated by the flash freezing/lyophilization process using wet-gel precursors, which were regenerated from a homogeneous dispersion of CNTs and cellulose in alkaline–urea aqueous solution. The morphology and textural properties of the resultant composite aerogels were investigated by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, Raman spectroscopy, and nitrogen adsorption–desorption tests. All the prepared materials exhibited both a nanostructured solid network (specific surface areas between 140 and 160 m2 g−1) and a nanoporous network (including macropores, mesopores and micropores). The results from thermogravimetric analysis and tensile tests revealed that the composite aerogels have good thermal stability and excellent mechanical properties, respectively. The Young's modulus of the composite aerogels could be tuned to reach about 90 MPa. And composite aerogels with 3–10 wt% CNTs have a conductivity of about 2.3 × 10−4 to 2.2 × 10−2 S cm−1, with the conductivity threshold at MWCNT volume fractions being as low as 3 × 10−3. Furthermore, these cellulose–CNT composite aerogels showed good sensitivity to ambient pressure.
Quenching of N(2D) by N2 and H2O

Quenching rate coefficients have been measured for interaction of N(2D) with N2 and with H2O. For N2, data were taken between 198 and 372 °K, and the rate expression is kN2=1.0×10−13 exp(−1020/RT) cm3 molec −1 ⋅sec−1. Reaction with H2O was measured only at 300 °K, and a rate coefficient of 2.5±0.5×10−10 cm3 molec−1 ⋅sec−1 was found. Earlier temperature‐dependent measurements of N(2D) quenching by N2O were checked and confirmed.
Synthesis and Thermal Properties of Epoxidized Vegetable Oil

Summary: The novel potential epoxy resins, epoxidized soybean oil (ESO) and epoxidized castor oil (ECO), were synthesized and characterized. The cationic polymerization of ESO and ECO with a latent thermal catalyst, N-benzylpyrazinium hexafluoroantimonate (BPH), was initiated at 80 and 50 °C, respectively. The cured ECO samples showed a higher Tg and lower coefficient of thermal expansion than those of ESO, due to the higher intermolecular interaction in the ECO/BPH system. Relationships between ESO or ECO conversion and temperature in the polymerization with 1 wt. -% BPH for 2 h.
High Temperature Stability Of N-decanethiolBy Adsorption On Nickel PowdersUsed As Reforming Catalysts InSolid Oxide Fuel Cells (SOFC)

Solid Oxide Fuel Cells (SOFC) are among the most promising electricity producing technologies. Their successful implementation and versatility depends upon a number of still not fully solved technological issues; namely, the possibility of using both fossil and renewable energy vectors is one of the most significant. Such energy vectors, constituting a crucial SOFC feedstock, are the natural gas, the biogas and all forms of fossil and renewable liquid fuels like diesel, biodiesel, “green” diesel. These fuels require a conversion step because SOFC can use only H2 and CO. This conversion step is achieved through catalytic reforming and/or partial oxidation. The most successful catalysts are ceramic-supported bimetallic composites. The presence of sulphur in the fuels is detrimental to the catalysts and constitutes one of the basic hurdles in scaling-up and adopting commercially these conversion technologies. In this paper, the authors, based on their previous work on the role of sulphur on micronic Ni powders used as CH4 reforming catalysts, study the behaviour of medium size thiols adsorbed at the surface of the Ni powders and treated thermally in a differential reactor set-up. The Diffuse Reflectance Infra-red Fourier Transform Spectroscopy (DRIFTS) analyses before and after adsorption and thermal treatments have shown that these Ni surfaces stabilize the adsorbed hydrocarbon chains and alter their catalytic properties during reforming. These results are quite surprising and open the field to a positive synergy between surface chemists and engineers in an effort to better control the efficiency and improve the tolerance to sulphur of these reforming catalysts.
Field evaluation of native white grub bio-agent, Bacillus cereus strain WGPSB-2 in Uttarakhand Himalayas and its impact on soil microbiota

A microbial pathogen, Bacillus cereus strain WGPSB-2 isolated from mid hills of Uttarakhand Himalayas (1000-1500 amsl) had potential as a biocontrol agent against white grubs of the region. Thus, the pathogen’s ability to suppress grubs was investigated under field conditions over a period of five years in five villages of the region. A single dose annual application of WGPSB-2 as talc based formulation (1x1010 spores/g) at the rate of 5kg/ha showed a sharp decline in grub population over the years in all the targeted villages. Pit sampling data showed maximum grub reduction during initial years of treatment in all villages and found significant for time, villages and time X village interactions in repeated measures ANOVA. Twenty four months post-inoculation resilience took place in micropits tested for bioagent impact on micro-floral densities, resulting in similar/ positive structures of tested microbial populations. The substantial numerical reduction of grub density coupled with no negative impacts on soil inhabitant microbiota, signifies WGPSB-2 as a vibrant technological option in this ecologically sensitive region.
Investigation of α-Synuclein Fibril Structure by Site-directed Spin Labeling*

The misfolding and fibril formation of α-synuclein plays an important role in neurodegenerative diseases such as Parkinson disease. Here we used electron paramagnetic resonance spectroscopy, together with site-directed spin labeling, to investigate the structural features of α-synuclein fibrils. We generated fibrils from a total of 83 different spin-labeled derivatives and observed single-line, exchange-narrowed EPR spectra for the majority of all sites located within the core region of α-synuclein fibrils. Such exchange narrowing requires the orbital overlap between multiple spin labels in close contact. The core region of α-synuclein fibrils must therefore be arranged in a parallel, in-register structure wherein same residues from different molecules are stacked on top of each other. This parallel, in-register core region extends from residue 36 to residue 98 and is tightly packed. Only a few sites within the core region, such as residues 62–67 located at the beginning of the NAC region, as well as the N- and C-terminal regions outside the core region, are significantly less ordered. Together with the accessibility measurements that suggest the location of potential β-sheet regions within the fibril, the data provide significant structural constraints for generating three-dimensional models. Furthermore, the data support the emerging view that parallel, in-register structure is a common feature shared by a number of naturally occurring amyloid fibrils.
PREPARATIVE ISOLATION OF BAICALIN AND WOGONOSIDE FROM SCUTELLARIAE RADIX BY HIGH SPEED COUNTER-CURRENT CHROMATOGRAPHY BY USING IONIC LIQUIDS AS THE MODIFIER OF TWO-PHASE SOLVENT SYSTEM

A preparative high speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoid compounds from Scutellariae Radix, the roots of Chinese medicinal plant Scutellaria baicalensis Georgi, was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Baicalin and wogonoside were purified from the crude extract of Scutellariae Radix by using ethyl acetate-water-[C4mim][PF6] (5:5:0.2, v/v) as two-phase solvent system. From 120 mg of the crude sample, 50.1 mg of baicalin and 45.6 mg of wogonoside were obtained in a one step separation. The purities of baicalin and wogonoside were 99.3% and 99.1%, respectively, as determined by HPLC area normalization method. The chemical structures of the isolated compounds were identified by1H-NMR and 13C-NMR.