Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel Essential Amino Acid Supplements Following Resistance Exercise Induce Aminoacidemia and Enhance Anabolic Signaling Irrespective of Age: A Proof-of-Concept Trial

We investigated the effects of ingesting a leucine-enriched essential amino acid (EAA) gel alone or combined with resistance exercise (RE) versus RE alone (control) on plasma aminoacidemia and intramyocellular anabolic signaling in healthy younger (28 ± 4 years) and older (71 ± 3 years) adults. Blood samples were obtained throughout the three trials, while muscle biopsies were collected in the postabsorptive state and 2 h following RE, following the consumption of two 50 mL EAA gels (40% leucine, 15 g total EAA), and following RE with EAA (combination (COM)). Protein content and the phosphorylation status of key anabolic signaling proteins were determined via immunoblotting. Irrespective of age, during EAA and COM peak leucinemia (younger: 454 ± 32 µM and 537 ± 111 µM; older: 417 ± 99 µM and 553 ± 136 µM) occurred ~60–120 min post-ingestion (younger: 66 ± 6 min and 120 ± 60 min; older: 90 ± 13 min and 78 ± 12 min). In the pooled sample, the area under the curve for plasma leucine and the sum of branched-chain amino acids was significantly greater in EAA and COM compared with RE. For intramyocellular signaling, significant main effects were found for condition (mTOR (Ser2481), rpS6 (Ser235/236)) and age (S6K1 (Thr421/Ser424), 4E-BP1 (Thr37/46)) in age group analyses. The phosphorylation of rpS6 was of similar magnitude (~8-fold) in pooled and age group data 2 h following COM. Our findings suggest that a gel-based, leucine-enriched EAA supplement is associated with aminoacidemia and a muscle anabolic signaling response, thus representing an effective means of stimulating muscle protein anabolism in younger and older adults following EAA and COM.
Revisiting Membrane Microdomains and Phase Separation: A Viral Perspective

Retroviruses selectively incorporate a specific subset of host cell proteins and lipids into their outer membrane when they bud out from the host plasma membrane. This specialized viral membrane composition is critical for both viral survivability and infectivity. Here, we review recent findings from live cell imaging of single virus assembly demonstrating that proteins and lipids sort into the HIV retroviral membrane by a mechanism of lipid-based phase partitioning. The findings showed that multimerizing HIV Gag at the assembly site creates a liquid-ordered lipid phase enriched in cholesterol and sphingolipids. Proteins with affinity for this specialized lipid environment partition into it, resulting in the selective incorporation of proteins into the nascent viral membrane. Building on this and other work in the field, we propose a model describing how HIV Gag induces phase separation of the viral assembly site through a mechanism involving transbilayer coupling of lipid acyl chains and membrane curvature changes. Similar phase-partitioning pathways in response to multimerizing structural proteins likely help sort proteins into the membranes of other budding structures within cells.
Direct Oral Anticoagulants and Timing of Hip Fracture Surgery

Timely surgical intervention in hip fracture has been linked to improved outcomes. Direct Oral Anticoagulants (DOACs) are an emerging class of anticoagulants without evidence-based guidelines on surgical timing. This study aims to investigate how DOACs affect surgical timing and hence perioperative outcomes. A retrospective database/registry review was conducted for geriatric hip fracture patients aged 65 and over between 2011 and 2018. Primary outcome was 30-day mortality. Secondary outcomes included serious adverse events (SAE), transfusion and postoperative day (POD) 1 haemoglobin (Hb) levels. From a cohort of 3264 patients, 112 admitted subjects were taking DOACs; the annual proportion on DOACs increased over time. Mean time to surgery from last dose (Ts) was 2.2 (±1.0 SD) days. The primary outcome, 30-day mortality, occurred in 16 (14%) patients with secondary outcomes of SAEs in 25 (22%) patients and transfusion in 30 (27%) patients. Ts (days) did not significantly affect 30-day mortality (odds ratio (OR): 1.37, 95% confidence interval (CI): 0.80–2.33; p = 0.248), SAE (hazard ratio (HR): 1.03, 95% CI: 0.70–1.52; p = 0.885), transfusion (OR: 0.72 95% CI: 0.45 to 1.16; p = 0.177) or POD 1 Hb (OR: 1.99, 95% CI: −0.59 to 4.57; p = 0.129). Timing of surgery does not influence common surgical outcomes such as 30-day mortality, SAE, transfusion, and POD1 Hb in patients taking DOACs on admission.
Impact of a Culturally Adapted Version of Illness Management and Recovery on Israeli Arabs With Serious Mental Illness.

OBJECTIVE In recent years, there has been growing awareness of the need for cultural adaptation of evidence-based practices, which is essential for successful implementation in diverse cultural contexts. This study investigated the impact of a culturally adapted version of Illness Management and Recovery (IMR), an evidence-based practice developed in the United States, on Israeli Arabs with serious mental illness. METHODS Using a quasi-experimental design, we compared the outcomes of 86 people who completed the culturally adapted IMR version with outcomes from a matched control group (N=64) who received treatment as usual. RESULTS Mixed repeated-measures analyses of variance demonstrated significant improvements in the domains of recovery, hope, self-efficacy, and quality of life among those who completed culturally adapted IMR. CONCLUSIONS The culturally adapted Arabic version of IMR for Israeli Arabs was found to generate significant positive improvement. The findings are discussed with emphasis on the importance of cultural adaptation.
Field observations of long-period, surf-zone standing waves in relation to contrasting beach morphologies

Field observations of water-surface oscillation and horizontal flow velocity spectra were carried out in the surf zones of contrasting beach-inshore morphodynamic states. The observations were made under conditions of long-period (7.5-11 s), moderate-energy (height 1 .2-2.3 m) swell on different beaches encompassing the full range of states from the steep. reflective extreme to the flat, dissipative extreme and including intermediate states involving different scales of rhythmic surf-zone topography and rip circulations. Experiments on all types of topography reveal standing surf-zone oscillations at periods longer than incident wave period. At least some of these appear to be edge waves. The actual frequencies are dependent on the morphodynamic state of the surf zone and beach. The standing waves with shortest period are zero-mode subharmonic edge waves which consistently occur on highly reflective beaches where they cause cusps spaced at one-half the edge-wave length. The standing waves with lowest frequency were observed on the flattest and most dissipative beach and comprised a wide band of infragravity frequencies. These oscillations could cause the multiple parallel bars. Intermediate frequencies occur in the presence of the pronounced bar-trough and rhythmic topographies of intermediate morphodynamic states.
The effect of size on the timing of visually mediated escape behaviour in staghorn sculpin Leptocottus armatus

The effect of prey size on the timing of the startle response in the sculpin Leptocottus armatus was investigated. Escape responses were triggered visually by a looming image obtained using a computer-generated animation of an approaching black disk. The results showed that apparent looming threshold (T AL , i.e. the threshold at which the rate of change of the visual angle subtended by predator frontal profile onto the prey's eye triggers an escape response by the prey) decreased with increasing prey size. Distance travelled within a fixed time was unaffected by size. Theoretical considerations suggest that larger prey would need to travel a longer distance (and so they would need more time) in order to move their whole body outside the predator's approaching gape. Therefore, the scaling of T AL may be explained by taking into account both ultimate and proximate considerations that need not be mutually exclusive. At an ultimate level, lower T AL in larger fish may be explained in terms of offsetting the disadvantage of offering a larger volume to be intercepted by the predator. At a proximate level, T AL may be related to the fish's visual acuity, which is higher in larger fish.
Covid-19 containment measures adopted in bone marrow transplantation service.

OBJECTIVE To describe the experience of nursing, in adopting containment measures, in the care of patients undergoing hematopoietic stem cell transplantation to avoid COVID-19. METHODS Experience report. RESULTS Containment measures involve those recommended by major health organizations, such as hand hygiene, social isolation, identification and monitoring of suspected or confirmed cases; and also the local measures implemented in the health service, such as the reduction in the number of hospitalizations for transplantation, clinical screening of outpatients entering the service, monitoring of respiratory signs and symptoms, the allocation of specific isolation rooms for those suspected of the disease and testing of symptomatic patients. Final considerations: The nurse is responsible for the challenge of planning nursing care to prevent the spread of coronavirus in a high-risk population and to implement measures based on available evidence, periodically updated.
Finite-/Fixed-Time Synchronization of Delayed Coupled Discontinuous Neural Networks With Unified Control Schemes

In this article, it addresses the problem of finite-/fixed-time synchronization of delayed coupled discontinuous neural networks in the unified framework. To achieve the finite-/fixed-time synchronization and precise estimations of setting time, two novel different kinds of controllers are established, in which one is switching. Then, based on the finite-/fixed-time theorem and Lyapunov function theory, some useful criteria are obtained to select suitable controllers’ parameters, which can guarantee error systems converge in the finite time/fixed time with respect to coupled neural networks. Moreover, corresponding estimations of the setting time are also provided. Finally, two numerical examples are introduced to show the effectiveness of the proposed control protocols.
A case of validity evidence for the Interprofessional Attitudes Scale

ABSTRACT Numerous measures have been developed for the assessment of interprofessional attitudes, including the Interprofessional Attitudes Scale (IPAS). The purpose of this study was to assess, and contribute to, the validity evidence for the IPAS. The IPAS was used in a three-year longitudinal study to collect data from first year health professionals. Three forms of assessment were conducted to accrue validity evidence for the IPAS: Exploratory Factor Analysis, Item Analysis, and analysis using Item Response Theory. Data was collected from 337 participants over three years. Separately, and cumulatively, the Exploratory Factor Analysis, Item Analysis and analysis using Item Response Theory identified issues with the content, response process, internal structure, and consequential validity of the IPAS. The outcomes of the present study call into question the use of the IPAS, and other measures, for the assessment of interprofessional attitudes. The present study contributes several pieces of evidence to the validity of the IPAS and the reevaluation of the use of attitude assessment in interprofessional education.
Characterising the increasing prevalence of crystal methamphetamine use in Vancouver, Canada, from 2006-2017: A gender-based analysis.

INTRODUCTION Methamphetamine use is increasing in North America and is associated with numerous negative health effects and social harms. As drug-related harms are often gendered, this study sought to examine the differential impacts of gender on the trends, risk factors and health implications of crystal methamphetamine use among a Canadian cohort of people with high rates of injection polysubstance use. METHODS Data were collected from two prospective cohorts of people who inject drugs in Vancouver, Canada. We examined trends in reported crystal methamphetamine use from 2006-2017 using the Cochrane-Armitage test for trend. We used multivariable generalised estimating equations, stratified by gender, to determine independent risk factors and health outcomes associated with crystal methamphetamine use. RESULTS In total, 1984 participants were included in the study, including 694 (35%) women. The prevalence of reported crystal methamphetamine use within the previous 6 months rose from 19% in 2006 to 36% in 2017, with a rise seen in both genders (P < 0.001). Reported crystal methamphetamine use was associated with numerous risk behaviours and drug-related harms in both genders, and in women it was associated with an increase in experiences of sexual violence (adjusted odds ratio 1.64, 95% confidence interval 1.18, 2.27). CONCLUSIONS These findings demonstrate the rising prevalence of reported crystal methamphetamine use in Vancouver in a cohort with high rates of polysubstance use. This work highlights drug and sex-related risk behaviours associated with crystal methamphetamine use for both genders, and underscores the need to develop strategies to address the growing use of crystal methamphetamine.
Patch testing with a textile dye mix with and without Disperse Orange 3

The textile dye mix (TDM) 6.6% pet. contains Disperse Blue (DB) 35, Disperse Yellow 3, Disperse Orange (DO) 1 and 3, Disperse Red 1 and 17, and DB 106 and 124. The most frequent allergen in TDM‐positive patients is DO 3. Around 85% of p‐phenylenediamine (PPD)‐allergic dermatitis patients have shown positive patch test reactions to DO 3. There has been a discussion to exclude DO 3 from TDM 6.6% because of frequent, strong reactions to TDM 6.6% and PPD.
PHYSIOLOGICAL RESPONSES OF WHEAT (TRITICUM AESTIVUM L.) GENOTYPES UNDER WATER STRESS CONDITIONS AT SEEDLING STAGE

Twelve wheat genotypes/lines were tested for different levels, 0.00 (control), -0.5 and -0.75 MPa of osmotic stresses (PEG-6000) for 20 days in a growth cabinet under a 10 h photoperiod (41.69 μmol m -2 s -1 ) to study growth and physiological attributes including nitrate reductase activity (NRA), proline and potassium contents. It was observed that root and shoot length, NRA and potassium (K + ) contents decreased however the proline contents increased by the imposition of osmotic stress. Comparatively less reduction in all these attributes and ion contents was observed in tolerant genotypes.
Pulmonary Angiopathy in Severe COVID-19: Physiologic, Imaging, and Hematologic Observations

Rationale: Clinical and epidemiologic data in coronavirus disease (COVID-19) have accrued rapidly since the outbreak, but few address the underlying pathophysiology. Objectives: To ascertain the physiologic, hematologic, and imaging basis of lung injury in severe COVID-19 pneumonia. Methods: Clinical, physiologic, and laboratory data were collated. Radiologic (computed tomography (CT) pulmonary angiography [n = 39] and dual-energy CT [DECT, n = 20]) studies were evaluated: observers quantified CT patterns (including the extent of abnormal lung and the presence and extent of dilated peripheral vessels) and perfusion defects on DECT. Coagulation status was assessed using thromboelastography. Measurements and Results: In 39 consecutive patients (male:female, 32:7; mean age, 53 ± 10 yr [range, 29–79 yr]; Black and minority ethnic, n = 25 [64%]), there was a significant vascular perfusion abnormality and increased physiologic dead space (dynamic compliance, 33.7 ± 14.7 ml/cm H2O; Murray lung injury score, 3.14 ± 0.53; mean ventilatory ratios, 2.6 ± 0.8) with evidence of hypercoagulability and fibrinolytic “shutdown”. The mean CT extent (±SD) of normally aerated lung, ground-glass opacification, and dense parenchymal opacification were 23.5 ± 16.7%, 36.3 ± 24.7%, and 42.7 ± 27.1%, respectively. Dilated peripheral vessels were present in 21/33 (63.6%) patients with at least two assessable lobes (including 10/21 [47.6%] with no evidence of acute pulmonary emboli). Perfusion defects on DECT (assessable in 18/20 [90%]) were present in all patients (wedge-shaped, n = 3; mottled, n = 9; mixed pattern, n = 6). Conclusions: Physiologic, hematologic, and imaging data show not only the presence of a hypercoagulable phenotype in severe COVID-19 pneumonia but also markedly impaired pulmonary perfusion likely caused by pulmonary angiopathy and thrombosis.
Effectiveness of kinesio taping on postoperative morbidity after impacted mandibular third molar surgery: a prospective, randomized, placebo-controlled clinical study

Abstract Objective Our study seeks to investigate the effectiveness of kinesio taping (KT) on postoperative morbidity compared to placebo and control groups after impacted third molar surgery. Methodology Sixty patients with impacted mandibular third molar were included in this prospective, randomized, placebo-controlled clinical study. After surgical extraction of the impacted tooth, patients were allocated into three groups (20 patients each): group 1 received KT (kinesio), group 2 received placebo taping (placebo), and group 3 received no taping (control). The groups were compared regarding facial swelling, pain and trismus. Swelling was evaluated using a tape measuring method. Pain was assessed by a visual analog scale and the number of analgesic tablets taken. Trismus was determined by measuring maximum mouth opening. Results In the KT group, all parameters reduced significantly on 2nd and 4th postoperative days compared to other groups; however, placebo and control groups revealed comparable outcomes. On 7th day, all groups showed comparable results. Conclusions The KT application is an effective method for reducing morbidity after impacted mandibular third molar surgery. However, placebo taping is not as effective as proper taping. Placebo taping shows similar results compared to no taping regarding facial swelling percentage, pain and trismus.
Functional Organization for Response Inhibition in the Right Inferior Frontal Cortex of Individual Human Brains

Abstract The right inferior frontal cortex (IFC) is critical to response inhibition. The right IFC referred in the human studies of response inhibition is located in the posterior part of the inferior frontal gyrus and the surrounding regions and consists of multiple areas that implement distinct functions. Recent studies using resting-state functional connectivity have parcellated the cerebral cortex and revealed across-subject variability of parcel-based cerebrocortical networks. However, how the right IFC of individual brains is functionally organized and what functional properties the IFC parcels possess regarding response inhibition remain elusive. In the present functional magnetic resonance imaging study, precision functional mapping of individual human brains was adopted to the parcels in the right IFC to evaluate their functional properties related to response inhibition. The right IFC consisted of six modules or subsets of subregions, and the spatial organization of the modules varied considerably across subjects. Each module revealed unique characteristics of brain activity and its correlation to behavior related to response inhibition. These results provide updated functional features of the IFC and demonstrate the importance of individual-focused approaches in studying response inhibition in the right IFC.
Capnocytophaga sputigena bloodstream infection in hematopoietic stem cell transplantations: two cases report and review of the literature

ABSTRACT Capnocytophaga is a group of facultative anaerobic gram-negative bacteria present in the oral cavity of humans, dogs and cats, as part of their normal oral flora. Here, we described two cases of bloodstream infections (BSI) caused by Capnocytophaga in neutropenic autologous hematopoietic stem cell transplantation (auto-HSCT) patients with mucositis (Grade I and Grade III) identified by Maldi-Tof. They were successfully treated with β-lactam (meropenem and piperacillin-tazobactam). The species C. sputigena was confirmed by 16S rRNA gene sequencing in one patient. The review of literature showed that C. ochraceae was the most frequent species causing BSI in auto-HSCT patients and that the patients usually presented mucositis and were neutropenic at the onset of the infection.
Using Intensive Longitudinal Data to Identify Early Predictors of Suicide-Related Outcomes in High-Risk Adolescents: Practical and Conceptual Considerations

Mobile technology offers new possibilities for assessing suicidal ideation and behavior in real- or near-real-time. It remains unclear how intensive longitudinal data can be used to identify proximal risk and inform clinical decision making. In this study of adolescent psychiatric inpatients (N = 32, aged 13-17 years, 75% female), we illustrate the application of a three-step process to identify early signs of suicide-related crises using daily diaries. Using receiver operating characteristic (ROC) curve analyses, we considered the utility of 12 features—constructed using means and variances of daily ratings for six risk factors over the first 2 weeks postdischarge (observations = 360)—in identifying a suicidal crisis 2 weeks later. Models derived from single risk factors had modest predictive accuracy (area under the ROC curve [AUC] 0.46-0.80) while nearly all models derived from combinations of risk factors produced higher accuracy (AUCs 0.80-0.91). Based on this illustration, we discuss implications for clinical decision making and future research.
Geographic variation and local adaptation in Oryza rufipogon across its climatic range in China

Variation in plant functional traits and demographic behaviour in populations of widely distributed species on geographic gradients may be key to understanding their geographic range limits. Oryza rufipogon, a wild progenitor of rice, occurs in discrete wetland populations over a wide latitudinal range in China. We examined correlations between plant functional traits, latitude, longitude and climatic variables in 34 populations of O. rufipogon across its northern range limit. We also investigated the responses of individuals from all populations after transplantation to two experimental gardens: one north of its range and the other in the extreme south. Seed mass in the field was positively correlated with latitude and longitude, whereas flag‐leaf area was negatively correlated with latitude; both effects could be explained largely as a response to temperature. Transplantation revealed that both plasticity and population differentiation contributed to geographic variation. All populations reproduced and overwintered in the southern garden, but in the northern garden, only populations from the northern range were able to reproduce (65%) and survive the winter (58%). Synthesis: Environmentally determined variation in plant functional traits is complex, with both plastic trade‐offs between traits and genetic differentiation between populations contributing to the location of the northern limit of Oryza rufipogon.