Miracle-dz/UniFAR-FTUs huggingface dataset:
Insects are a viable protein source for human consumption: from insect protein digestion to postprandial muscle protein synthesis in vivo in humans: a double-blind randomized trial

ABSTRACT Background Insects have recently been identified as a more sustainable protein-dense food source and may represent a viable alternative to conventional animal-derived proteins. Objectives We aimed to compare the impacts of ingesting lesser mealworm– and milk-derived protein on protein digestion and amino acid absorption kinetics, postprandial skeletal muscle protein synthesis rates, and the incorporation of dietary protein–derived amino acids into de novo muscle protein at rest and during recovery from exercise in vivo in humans. Methods In this double-blind randomized controlled trial, 24 healthy, young men ingested 30 g specifically produced, intrinsically l-[1-13C]-phenylalanine and l-[1-13C]-leucine labeled lesser mealworm– or milk-derived protein after a unilateral bout of resistance-type exercise. Primed continuous l-[ring-2H5]-phenylalanine, l-[ring-3,5-2H2]-tyrosine, and l-[1-13C]-leucine infusions were applied, with frequent collection of blood and muscle tissue samples. Results A total of 73% ± 7% and 77% ± 7% of the lesser mealworm and milk protein–derived phenylalanine was released into the circulation during the 5 h postprandial period, respectively, with no significant differences between groups (P < 0.05). Muscle protein synthesis rates increased after both lesser mealworm and milk protein concentrate ingestion from 0.025 ± 0.008%/h to 0.045 ± 0.017%/h and 0.028 ± 0.010%/h to 0.056 ± 0.012%/h at rest and from 0.025 ± 0.012%/h to 0.059 ± 0.015%/h and 0.026 ± 0.009%/h to 0.073 ± 0.020%/h after exercise, respectively (all P < 0.05), with no differences between groups (both P > 0.05). Incorporation of mealworm and milk protein-derived l-[1-13C]-phenylalanine into de novo muscle protein was greater after exercise than at rest (P < 0.05), with no differences between groups (P > 0.05). Conclusions Ingestion of a meal-like amount of lesser mealworm–derived protein is followed by rapid protein digestion and amino acid absorption and increases muscle protein synthesis rates both at rest and during recovery from exercise. The postprandial protein handling of lesser mealworm does not differ from ingesting an equivalent amount of milk protein concentrate in vivo in humans. This trial was registered at www.trialregister. nl as NL6897.
Repeatability and agreement of biometric measurements using spectral domain anterior segment optical coherence tomography and Scheimpflug tomography in keratoconus

Purpose To compare the repeatability and agreement in biometric measurements using Spectral Domain Anterior Segment OCT (AS-OCT, REVO-NX, Optopol) and Scheimpflug tomography (Pentacam-AXL, Oculus) in keratoconus. Methods Prospective case series at a university hospital tertiary center. Axial length (AL), anterior chamber depth (ACD), central corneal thickness (CCT), and thinnest corneal thickness (TCT) were measured using both devices in patients with keratoconus. Three groups were analyzed: eyes with no prior crosslinking or contact lens wear (Group A), eyes with prior crosslinking (Group B), and eyes with prior contact lens wear (Group C). Repeatability and agreement of measurements were analyzed. Results The study comprised of 214 eyes of 157 subjects. In Group A (n = 95 eyes), Group B (n = 86 eyes), and Group C (n = 33 eyes), intraclass correlation coefficient (ICC) was higher than 0.90 for all examined parameters, except for ACD readings in Group A with the REVO-NX (ICC = 0.83). Differences in ACD, TCT, and CCT were significantly different between the two devices for Groups A, B and C (p<0.05). AL measurements differed significantly in Groups A and B (p<0.05) but not in Group C (p = 0.18). Repeatability did not vary significantly between Groups A, B, or C in any parameter with both devices (p>0.05). There was poor agreement between the two devices across all parameters (p<0.05). Conclusions Both devices demonstrated good repeatability but poor agreement across AL, ACD, CCT and TCT measurements. There was no significant difference in repeatability in virgin eyes compared to eyes with prior crosslinking or contact lens wear, however, the interchangeable use of the two devices is not recommended.
Management of Pea Leaf Weevil (Coleoptera: Curculionidae) and Development of a Nominal Threshold in Faba Beans

Abstract Pea leaf weevil, Sitona lineatus (L.) (Coleoptera: Curculionidae), can reduce the yield of field pea [Pisum sativum (L.) (Fabales: Fabaceae)] and faba bean [Vicia faba (L.) (Fabales: Fabaceae)]. Adults feed on the foliage and larvae feed on root nodules and nodule-associated Rhizobium Frank (Rhizobiales: Rhizobiacea) bacteria. In this study, we developed a data-based nominal threshold for pea leaf weevil in faba bean. We further tested the efficacy of insecticidal seed treatment and foliar insecticide (thiamethoxam and lambda-cyhalothrin, respectively), and nitrogen amendment for pea leaf weevil control using a multi-year field plot study at two sites in Alberta, Canada. Pea leaf weevil feeding damage significantly reduced faba bean yields. Thiamethoxam reduced adult and larval damage, and protected faba bean yield, while neither lambda-cyhalothrin nor a nitrogen amendment was effective in protecting yield.The percentage of seedlings with feeding on the terminal leaf had a negative relationship with yield and was used to estimate a nominal threshold near 15% of seedlings with terminal leaf damage. Since lambda-cyhalothrin is not effective in managing pea leaf weevil on faba bean, there is a need to research additional integrated pest management strategies to reduce prophylactic insecticidal seed treatments.
Using the adherence‐efficacy relationship of emtricitabine and tenofovir disoproxil fumarate to calculate background hiv incidence: a secondary analysis of a randomized, controlled trial

Randomized trials of new agents for HIV pre‐exposure prophylaxis (PrEP) compare against emtricitabine and tenofovir disoproxil fumarate (F/TDF), without a placebo group. We used the well‐characterized adherence‐efficacy relationship for F/TDF to back‐calculate the (non‐PrEP) counterfactual background HIV incidence (bHIV) in a randomized trial of a novel PrEP agent and estimate comparative efficacy (to counterfactual bHIV).
Independent effects of secondary hyperparathyroidism and hyperphosphatemia on chronic kidney disease progression and cardiovascular events: an analysis from the NEFRONA cohort.

BACKGROUND Secondary hyperparathyroidism (SHPT) is a complication of chronic kidney disease (CKD), and it is associated with changes in calcium and phosphate. These related changes have been associated with increased cardiovascular mortality and CKD progression. It is not clear whether negative outcomes linked to SHPT are confounded by such factors. The present study was designed to assess the possible independent effects of SHPT (defined as patients with excessive PTH levels or on treatment with PTH reducing agents) on the risk of CKD progression and CVE incidence in CKD patients, as well as whether hypercalcemia and/or hyperphosphatemia act as effect modifiers. METHODS The study enrolled 2445 CKD patients without previous CVE from the NEFRONA cohort (950 stage 3, 612 stage 4, 195 stage 5 and 688 on Dialysis). Multivariate logistic and Fine and Gray regression analysis were used to determine the risk of patients of suffering CKD progression or a CVE. RESULTS Prevalence of SHPT in the whole cohort was 65.6% (CKD 3 54.7%; CKD 4 74.7%; CKD 5 71.4%; Dialysis 68.6%). After 2-years, 301 patients presented CKD progression. During 4-years follow-up, 203 CVE were registered. Patients with SHPT showed a higher adjusted risk for CKD progression and CVE. Furthermore, hyperphosphatemia was shown to be an independent risk factor in both outcomes and did not modify SHPT effect. No significant interactions were detected between the presence of SHPT and hypercalcemia or hyperphosphatemia. CONCLUSIONS We conclude that SHPT and hyperphosphatemia are independently associated with CKD progression and the incidence of CVE in CKD patients.
Statistical methods versus machine learning techniques for donor-recipient matching in liver transplantation

Donor-Recipient (D-R) matching is one of the main challenges to be fulfilled nowadays. Due to the increasing number of recipients and the small amount of donors in liver transplantation, the allocation method is crucial. In this paper, to establish a fair comparison, the United Network for Organ Sharing database was used with 4 different end-points (3 months, and 1, 2 and 5 years), with a total of 39, 189 D-R pairs and 28 donor and recipient variables. Modelling techniques were divided into two groups: 1) classical statistical methods, including Logistic Regression (LR) and Naïve Bayes (NB), and 2) standard machine learning techniques, including Multilayer Perceptron (MLP), Random Forest (RF), Gradient Boosting (GB) or Support Vector Machines (SVM), among others. The methods were compared with standard scores, MELD, SOFT and BAR. For the 5-years end-point, LR (AUC = 0.654) outperformed several machine learning techniques, such as MLP (AUC = 0.599), GB (AUC = 0.600), SVM (AUC = 0.624) or RF (AUC = 0.644), among others. Moreover, LR also outperformed standard scores. The same pattern was reproduced for the others 3 end-points. Complex machine learning methods were not able to improve the performance of liver allocation, probably due to the implicit limitations associated to the collection process of the database.
A practical individualized radiation precaution based on the dose rate at release time after inpatient 131I ablation therapy

Introduction Retained radioactivity of 131I after ablation therapy largely differs in each patient according to factors including the amount of remnant thyroid tissue, renal function, and use of recombinant human thyroid-stimulating hormone. To reduce unnecessary restriction of patient’s daily life after inpatient 131I ablation therapy, we propose a practical individualized method for radiation precaution based on dose rate at release time. Methods We evaluated 215 patients with differentiated thyroid cancer who underwent inpatient 131I ablation therapy following total thyroidectomy. Effective dose equivalent rates at 1-m distance were measured upon release (EDRR) on day 2 and during delayed whole-body scan (EDRD) visits on day 6‒8 after 131I administration. The biexponential model was designed to estimate total effective dose equivalent to others. To assess conservativeness of our model, EDRD estimated by our model was compared with measured EDRD. EDRR-based periods of precaution not to receiving 1 mSv of radiation exposure were estimated and compared with those based on administered radioactivities on American Thyroid Association (ATA) recommendations. Results The EDRR ranged from 1.0–48.9 μSv/hr. The measured EDRD were equal to or lower than estimated EDRD in all patients, except for one, indicating that our model is sufficiently conservative. According to our model, no subjects needed additional daytime restriction after release. The maximum permissible times for public transportation use were longer in all patients compared with those based on administered radioactivities. Nighttime restriction periods were significantly shorter than those based on administered radioactivity; median periods requiring sleeping apart were 0 (range, 0‒5), 4 (range, 1‒14), and 3 (range, 2‒13) days after release in patients treated with radioactivity doses of 2.96, 5.50, and 7.40 GBq, respectively, needing 8, 16, and 19 additional days, respectively, based on administered radioactivity. Conclusions Radiation safety instructions using proposed method based on EDRR of individual patient could safely reduce the burden of radiation precaution.
Elementary mechanisms of calmodulin regulation of NaV1.5 producing divergent arrhythmogenic phenotypes

Significance Calmodulin (CaM) regulation of cardiac NaV channels is vital for cardiac physiology and pathophysiology. Channelopathic mutations in NaV1.5 that disrupt CaM binding trigger two mechanistically divergent arrhythmia syndromes. Specifically, long QT syndrome 3 results from a gain-of-channel function, while Brugada syndrome stems from a loss-of-channel function. Yet, mechanisms that elicit seemingly paradoxical changes in channel function are unknown. Using single-channel analysis, we demonstrate that the disruption of CaM binding to NaV1.5 diminishes channel activity and enhances the propensity for persistent Na+ current, all resulting from a switch in the NaV inactivation mechanism. These findings reveal insights into the mechanism of CaM regulation of NaV channels as well as inform upon alterations in channel function that trigger life-threatening arrhythmias. In cardiomyocytes, NaV1.5 channels mediate initiation and fast propagation of action potentials. The Ca2+-binding protein calmodulin (CaM) serves as a de facto subunit of NaV1.5. Genetic studies and atomic structures suggest that this interaction is pathophysiologically critical, as human mutations within the NaV1.5 carboxy-terminus that disrupt CaM binding are linked to distinct forms of life-threatening arrhythmias, including long QT syndrome 3, a “gain-of-function” defect, and Brugada syndrome, a “loss-of-function” phenotype. Yet, how a common disruption in CaM binding engenders divergent effects on NaV1.5 gating is not fully understood, though vital for elucidating arrhythmogenic mechanisms and for developing new therapies. Here, using extensive single-channel analysis, we find that the disruption of Ca2+-free CaM preassociation with NaV1.5 exerts two disparate effects: 1) a decrease in the peak open probability and 2) an increase in persistent NaV openings. Mechanistically, these effects arise from a CaM-dependent switch in the NaV inactivation mechanism. Specifically, CaM-bound channels preferentially inactivate from the open state, while those devoid of CaM exhibit enhanced closed-state inactivation. Further enriching this scheme, for certain mutant NaV1.5, local Ca2+ fluctuations elicit a rapid recruitment of CaM that reverses the increase in persistent Na current, a factor that may promote beat-to-beat variability in late Na current. In all, these findings identify the elementary mechanism of CaM regulation of NaV1.5 and, in so doing, unravel a noncanonical role for CaM in tuning ion channel gating. Furthermore, our results furnish an in-depth molecular framework for understanding complex arrhythmogenic phenotypes of NaV1.5 channelopathies.
Mechanical Control of Cell Migration by the Metastasis Suppressor Tetraspanin CD82/KAI1

The plasma membrane is a key actor of cell migration. For instance, its tension controls persistent cell migration and cell surface caveolae integrity. Then, caveolae constituents such as caveolin-1 can initiate a mechanotransduction loop that involves actin- and focal adhesion-dependent control of the mechanosensor YAP to finely tune cell migration. Tetraspanin CD82 (also named KAI-1) is an integral membrane protein and a metastasis suppressor. Its expression is lost in many cancers including breast cancer. It is a strong inhibitor of cell migration by a little-known mechanism. We demonstrated here that CD82 controls persistent 2D migration of EGF-induced single cells, stress fibers and focal adhesion sizes and dynamics. Mechanistically, we found that CD82 regulates membrane tension, cell surface caveolae abundance and YAP nuclear translocation in a caveolin-1-dependent manner. Altogether, our data show that CD82 controls 2D cell migration using membrane-driven mechanics involving caveolin and the YAP pathway.
Corporate Governance and Pollution Externalities of Public and Private Firms*

The number of U.S. publicly traded firms has halved in 20 years. How will this shift in ownership structure affect the economy’s externalities? Using comprehensive data on greenhouse gas emissions from 2007 to 2016, we find that independent private firms are less likely to pollute and incur EPA penalties than are public firms, and we find no differences between private sponsor-backed firms and public firms, controlling for industry, time, location, and a host of firm characteristics. Within public firms, we find a negative association between emissions and mutual fund ownership and board size, suggesting that increased oversight may decrease externalities.
Changes in Accrual Properties and Operating Environment: Implications for Cash Flow Predictability

Abstract This paper reconciles conflicting evidence in prior literature on the relative ability of earnings and cash flows in predicting future cash flows. Further, we investigate the implications of temporal shifts in accrual properties and operating environment for cash flow predictability. Three key insights emerge. First, cash flows consistently outperform earnings in predicting future cash flows. Second, accruals and its components, including those capturing non-articulating events, have incremental (albeit small) predictive ability over cash flows. Third, earnings’ ability to predict future cash flows has increased over the period 1989–2015, due to changes in operating environment rather than accrual properties.
Do Equity Markets Care About Income Inequality? Evidence from Pay Ratio Disclosure

Do equity markets care about income inequality? We address this question by examining equity markets’ reaction and investors’ portfolio rebalancing in response to the first-time disclosure of the ratio of CEO to median worker pay by U.S. public companies in 2018. We find that firms’ disclosing higher pay ratios experience significantly lower abnormal announcement returns. Additional evidence suggests that equity markets “dislike” high pay dispersion rather than high CEO pay or low worker pay. Firms whose shareholders are more inequality-averse experience a more pronounced negative market response to high pay ratios compared to firms with less inequality-averse shareholders. Finally, we find that during 2018 more inequality-averse investors rebalance their portfolios away from high pay ratio stocks relative to other investors. Overall, our results suggest that equity markets are concerned about high within-firm pay dispersion, and investors’ attitude towards income inequality is a channel through which high pay ratios negatively affect firm value.
Analysis of agricultural trade in China based on the theory of factor endowment

Purpose. The purpose of the paper is to present the results of the study of agricultural trade in China based on the theory of factor endowment and to develop a policy recommendation for the intensification of agricultural trade based on technological progress. Methodology / approach. This paper uses the methods of economic theory and comparative analysis to study the development of agricultural products trade in China; analysis the advantages of developing agricultural products trade in China by using comparative advantage theory; and econometric analysis to study the relationship between volume of agrarian trade and agricultural labor force, capital and land resources. Results. This paper uses time series data to analyze the three elements of capital, labor and land, and the development of agricultural trade in China. According to the established data model, it is obviously that China’s agricultural production of land factor endowment constraints are obvious, labor factor endowment is abundant but also facing the problem of increasing costs, labor personnel gradually decline. In this case, technological progress is more critical to the cultivation and maintenance of comparative advantage of agricultural products. The government should guide the direction of technological changes, technical support, capital support (material capital and human capital); and promote the rapid development of China’s agricultural trade. Originality / scientific novelty. Author’s developed econometric model is very useful in empirical assessment for the China agricultural products trade development, and it also provides new decisions for China to develop agricultural trade. Accordingly, the proposed method can provide an answer to the question of how to develop traditional agriculture in the context of rapid economic changes, and put forward more practical countermeasures. Practical value / significance. The main proposals can be used by the Government of China to formulate better agricultural trade policies and achieve rapid economic growth and increasing farmer incomes.
SNV Reoccurrence in Multiple Regions in the Genome of 2019-nCoV

The rapid development of 2019-2020 Wuhan seafood market pneumonia currently posed a major public health concern in China. Genome sequencing identified a novel beta-coronavirus closely related to SARS-CoV, named 2019-nCoV by WHO, as the cause of this pandemic disease. Viruses with single stranded RNA genome are prone to evolve quickly by accumulation of mutations, such as SNV, INDEL and cross viral recombination, aiding fast transmission among hosts and cross species. Here we collected related genome sequences and investigated variations shared by different strains of 2019-nCoV, identified reoccurrence of SNV mutations in clusters of patients, an indication of rapid evolution of 2019-nCoV at the transmission from animal host to human. The information collected herein would help to understand the dynamics of current pandemic.
Government Ownership and Corporate Tax Evasion: Evidence from China

We examine the effect of government ownership on tax evasion in China. After correcting for the partial observability of tax evasion, we find that state-owned enterprises (SOEs) are more likely to evade taxes and less likely to be detected than non-SOEs. After being caught for tax evasion, SOEs also pay lower penalties. We show that tax evasion is positively correlated with effective tax rates (ETRs) and the effect of government ownership on tax evasion is opposite the effect of government ownership on ETRs. Our results suggest that tax evasion is fundamentally different from normal tax avoidance typically measured by ETRs.
Tracing foot-and-mouth disease virus phylogeographical patterns and transmission dynamics.

Foot-and-mouth disease virus (FMDV) has proven its to propagate and international borders on numerous occasions, but yet details about the directionality of the spread along with the role of the different host in transmission remain unexplored. To elucidate FMDV global spread characteristics, we studied the spatiotemporal phylodynamics of serotypes O, A, Asia1, SAT1, SAT2, and SAT3, based on more than 50 years of phylogenetic and epidemiological information. Results Our results revealed phylogeographic patterns, dispersal rates, and the role of host species in the dispersal and maintenance of virus circulation. Contrary to previous studies, our results showed that three serotypes were monophyletic (O, A, and Asia1), while all SATs serotypes did not evidence a defined common ancestor. Root state posterior probability (RSPP) analysis suggested Belgium as the country of origin for serotype O (RSPP= 0.27). India was the ancestral country for serotypes A (RSPP= 0.28), and Asia-1 (RSPP= 0.34), while Uganda appeared as the most likely origin country of all SAT serotypes (RSPP> 0.45). Furthermore, we identified the key centers of dispersal of the virus, being China, India and Uganda the most important ones. Bayes factor analysis revealed cattle as the major source of the virus for most of the serotypes (RSPP> 0.63), where the most important host-species transition route for serotypes O, A, and Asia1 was from cattle Bos taurus to swine Sus scrofa domesticus (BF>500), while, for SAT serotypes was from B. taurus to African buffalo Syncerus caffer. This study provides significant insights into the spatiotemporal dynamics of the global circulation of FMDV serotypes, by characterizing the viral routes of spread at serotype level, especially uncovering the importance of host species for each serotype in the evolution and spread of FMDV which further improve future decisions for more efficient control and eradication.