Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of microalbuminuria with serum lipids and inflammatory markers in an adult population in the Dikgale Health and Demographic Surveillance System site, South Africa.

BACKGROUND There is evidence that microalbuminuria (urinary albumin excretion) is an early sign of vascular damage and an established risk factor for cardiovascular morbidity and mortality. This study investigated the magnitude of microalbuminuria and its association with serum lipids and inflammatory markers among a rural black population residing in the Dikgale Health and Demographic Surveillance System site, South Africa. METHODS Data were collected from 602 presumably healthy participants (225 men and 377 women) aged ≥ 18 years. Biochemical data collection included serum lipids, glucose, insulin, high-sensitivity C-reactive protein (hs-CRP), urine albumin and creatinine. Anthropometry and blood pressure were also measured. Microalbuminuria was diagnosed with an albumin-creatinine ratio of ≥ 2.5 mg/mmol in men and ≥ 3.5 mg/mmol in women. Data were analysed using SPSS version 22.0. RESULTS The mean age of participants was 48.63 ± 20.89 years. High percentages of microalbuminaria (35.7%), high levels of interleukin 6 (17.8%), hs-CRP (32.9%), triglycerides (TG) (26.1%), low-density lipoprotein cholesterol (52.2%) and total cholesterol (32.0%), and low levels of high-density lipoprotein cholesterol (29.1%) were observed in the population. Increased glucose levels (32.8%), insulin resistance (27.6%), hypertension (45.8%), overweight (26.8%) and obesity (25.4%) were also prevalent. Microalbuminuria was associated with high hs-CRP and TG levels in the men (adjusted odds ratios = 9.434, 95% confidence interval: 1.753 - 50.778, p = 0.01). CONCLUSIONS High prevalence of microalbuminuria, hypertension, insulin resistance, overweight and obesity, as well as abnormal levels of serum lipids and inflammatory markers were observed in the population. Microalbuminuria was associated with high hs-CRP and TG levels among men.
COVID-19 vaccine hesitancy between teachers and students in a college, a cross-sectional study in China

ABSTRACT Studies have identified teacher effects on students’ attitudes. This study explored the differences in and associations between teachers’ and students’ COVID-19 vaccine hesitancy, and the factors that affect it. A population-based self-administered online survey was conducted to evaluate the COVID-19 vaccine hesitancy of teachers and students in a college in Taizhou, China. A total of 835 valid questionnaires were obtained. All data were analyzed using IBM SPSS statistics 26.0 software. The proportions of COVID-19 vaccine hesitancy for teachers and students were 31.7%, and 23.8%, respectively. In the binary logistic regression analysis, teachers who perceived the safety of the COVID-19 vaccine as low (OR = 6.794 , 95%CI: 1.701–27.143), did not pay continuous attention to the vaccine news (OR = 3.498, 95%CI: 1.150–10.640), and suffered chronic diseases (OR = 2.659, 95%CI: 1.135–6.227) were more likely to hesitate to get vaccinated against COVID-19. The group of students who perceived the COVID-19 vaccine safety as being low (OR = 1.805, 95%CI: 1.094–2.979) were more hesitant to get the COVID-19 vaccine. While both teachers and students were hesitant about the COVID-19 vaccine, teachers were found to be more so. Perceptions regarding the safety of COVID-19 vaccines, attention to and awareness of vaccine news, and chronic medical conditions were the main factors that influenced the hesitation regarding COVID-19 vaccines. Therefore, students’ vaccine hesitancy may depend largely on the perceptions of the vaccine’s safety rather than teachers’ vaccine hesitancy.
Improving Cultural Competence and Self-Efficacy Among Postgraduate Nursing Students: Results of an Online Cultural Care Training Program

Introduction: Since nurses and nursing students interact with culturally diverse clients in health care settings, training programs are required to improve cultural competence and self-efficacy among them. This study aimed to investigate the effect of an online cultural care training program on cultural competence and self-efficacy among postgraduate nursing students. Methods: The present interventional study used random sampling to allocate 80 postgraduate nursing students into the intervention and control groups. Cultural care training program was held for the intervention group. Results: No significant difference in cultural competence and self-efficacy scores was found between the intervention and control groups in the pretest. However, the intervention group scored higher in terms of cultural competence and self-efficacy after the intervention. Discussion: Given cultural diversity and prevalence of medical tourism, nursing instructors need to pay special attention to cultural care education at all academic levels.
Stratum corneum hydration in healthy adult humans according to the skin area, age and sex: a systematic review and meta‐analysis

Stratum corneum (SC) hydration is one of the most important skin biophysical parameters, which is affected by age, sex and anatomic site. We conducted a systematic review and meta‐analysis to provide detailed information on baseline values of SC hydration in healthy human adults, according to the skin area, age and sex. The databases Medline, Embase, Web of Science and Scopus were searched for primary reports, with in vivo corneometry measurements, which were conducted on disease‐free skin of human subjects, aged 18+ years in English. Risk of bias was evaluated using the critical appraisal tools for JBI Systematic Reviews, and only low‐risk studies were selected. The comparison was performed in each skin area between males and females and also between young‐ and middle‐age subjects. Among 323 Retrieved titles, full texts of 259 articles were read, and 240 studies were excluded due to unclear or insufficient reporting of SC hydration, as well as high or medium risk of bias. 19 studies (including 113 records) providing data about SC hydration in 12 skin areas were included in the final data synthesis. Pooled sample sizes ranged from n = 32 for lips to a maximum of n = 3202 for cheeks. The lowest hydration of 12.5 [95% CI 11.05–13.94] was reported for the back, and the highest hydration of 64.34) [95 CI% 62.07–64.59] for the periorbital area. Facial skin showed higher water content compared with other areas. There were also site‐dependent differences in the hydration level of the facial skin. Comparisons between two age groups, and male and female participants, did not show any statistically significant differences. The main limitation was we included studies using only one measurement device. The quality of reporting SC hydration in humans should be increased in future studies.
Bile duct anastomosis does not promote bacterial contamination of autologous blood salvaged during living donor liver transplantation

Bile duct surgeries are conventionally considered to promote bacterial contamination of the surgical field. However, liver transplantation recipients' bile produced by the newly implanted liver graft from healthy living donors may be sterile. We tested bacterial contamination of autologous blood salvaged before and after bile duct anastomosis (BDA) during living donor liver transplantation (LDLT). In 29 patients undergoing LDLT, bacterial culture was performed for four blood samples and one bile sample: two from autologous blood salvaged before BDA (one was nonleukoreduced and another was leukoreduced), two from autologous blood salvaged after BDA (one was nonleukoreduced and another was leukoreduced), and one from bile produced in the newly implanted liver graft. The primary outcome was bacterial contamination. The risk of bacterial contamination was not significantly different between nonleukoreduced autologous blood salvaged before BDA and nonleukoreduced autologous blood salvaged after BDA (44.8% and 31.0%; odds ratio 0.33, 95% confidence interval 0.03–1.86; p = 0.228). No bacteria were found after leukoreduction in all 58 autologous blood samples. All bile samples were negative for bacteria. None of the 29 patients, including 13 patients who received salvaged autologous blood positive for bacteria, developed postoperative bacteremia. We found that bile from the newly implanted liver graft is sterile in LDLT and BDA does not increase the risk of bacterial contamination of salvaged blood, supporting the use of blood salvage during LDLT even after BDA. Leukoreduction converted all autologous blood samples positive for bacteria to negative. The clinical benefit of leukoreduction for salvaged autologous blood on post‐LDLT bacteremia needs further research.
COMPO*SED: Composite Parallel Coordinates for Co-Dependent Multi-Attribute Choices

We propose Composite Parallel Coordinates, a novel parallel coordinates technique to effectively represent the interplay of component alternatives in a system. It builds upon a dedicated data model that formally describes the interaction of components. Parallel coordinates can help decision-makers identify the most preferred solution among a number of alternatives. Multi-component systems require one such multi-attribute choice for each component. Each of these choices might have side effects on the system's operability and performance, making them co-dependent. Common approaches employ complex multi-component models or involve back-and-forth iterations between single components until an acceptable compromise is reached. A simultaneous visual exploration across independently modeled but connected components is needed to make system design more efficient. Using dedicated layout and interaction strategies, our Composite Parallel Coordinates allow analysts to explore both individual properties of components as well as their interoperability and joint performance. We showcase the effectiveness of Composite Parallel Coordinates for co-dependent multi-attribute choices by means of three real-world scenarios from distinct application areas. In addition to the case studies, we reflect on observing two domain experts collaboratively working with the proposed technique and communicating along the way.
SELWAK: A Secure and Efficient Lightweight and Anonymous Authentication and Key Establishment Scheme for IoT Based Vehicular Ad hoc Networks

In recent decades, Vehicular Ad Hoc Networks (VANET) have emerged as a promising field that provides real-time communication between vehicles for comfortable driving and human safety. However, the Internet of Vehicles (IoV) platform faces some serious problems in the deployment of robust authentication mechanisms in resource-constrained environments and directly affects the efficiency of existing VANET schemes. Moreover, the security of the information becomes a critical issue over an open wireless access medium. In this paper, an efficient and secure lightweight anonymous mutual authentication and key establishment (SELWAK) for IoT-based VANETs is proposed. The proposed scheme requires two types of mutual authentication: V2V and V2R. In addition, SELWAK maintains secret keys for secure communication between Roadside Units (RSUs). The performance evaluation of SELWAK affirms that it is lightweight in terms of computational cost and communication overhead because SELWAK uses a bitwise Exclusive-OR operation and one-way hash functions. The formal and informal security analysis of SELWAK shows that it is robust against man-in-the-middle attacks, replay attacks, stolen verifier attacks, stolen OBU attacks, untraceability, impersonation attacks, and anonymity. Moreover, a formal security analysis is presented using the Real-or-Random (RoR) model.
Treatment Outcomes Among Pregnant Patients With Multidrug-Resistant Tuberculosis

Key Points Question What are the treatment outcomes and adverse events among pregnant patients with multidrug-resistant tuberculosis (MDR-TB)? Findings In a systematic review and meta-analysis of 10 studies including 275 pregnant patients with MDR-TB, the pooled proportion of treatment success was 72.5%, and the pooled proportion of favorable pregnancy outcomes was 73.2%. Adverse events, such as liver function impairment, kidney function impairment, hearing loss, and hypokalemia, were common among pregnant patients with MDR-TB, occurring in more than half of the patients. Meaning This study suggests that a high rate of treatment success and favorable pregnancy outcomes can be achieved when pregnant patients with MDR-TB are treated with effective regimens.
Deception Cues During High-Risk Situations: 911 Homicide Calls

During everyday interactions, cues tend to be weakly related to deception. However, there are theoretical reasons to suspect that such cues will be more prominent during high-risk interactions. The current study explored deception cues during one particular high-risk interaction—911 homicide calls placed by adults. In Sample 1, judges coded 911 homicide calls (n = 82) by Q-sorting 86 cues. Results indicated that deceptive callers tended to display emotional cues (e.g., self-dramatizing, moody, worried, emotional, nervous), appeared overwhelmed, and related narratives that lacked structure, clarity, and focus. Judges coded a separate sample of 911 calls (n = 64), and deception scores were computed using a template-matching approach based on the findings from Sample 1. Results indicated that deceptive 911 callers had higher deception scores than honest callers. The effect sizes yielded in this study highlight the relevance of deception cues during high-risk interactions and the usefulness of the person-centered Q-sort method.
Trade-Offs Predicted by Metabolic Network Structure Give Rise to Evolutionary Specialization and Phenotypic Diversification

Abstract Mitigating trade-offs between different resource-utilization functions is key to an organism’s ecological and evolutionary success. These trade-offs often reflect metabolic constraints with a complex molecular underpinning; therefore, their consequences for evolutionary processes have remained elusive. Here, we investigate how metabolic architecture induces resource-utilization constraints and how these constraints, in turn, elicit evolutionary specialization and diversification. Guided by the metabolic network structure of the bacterium Lactococcus cremoris, we selected two carbon sources (fructose and galactose) with predicted coutilization constraints. By evolving L. cremoris on either fructose, galactose, or a mix of both sugars, we imposed selection favoring divergent metabolic specializations or coutilization of both resources, respectively. Phenotypic characterization revealed the evolution of either fructose or galactose specialists in the single-sugar treatments. In the mixed-sugar regime, we observed adaptive diversification: both specialists coexisted, and no generalist evolved. Divergence from the ancestral phenotype occurred at key pathway junctions in the central carbon metabolism. Fructose specialists evolved mutations in the fbp and pfk genes that appear to balance anabolic and catabolic carbon fluxes. Galactose specialists evolved increased expression of pgmA (the primary metabolic bottleneck of galactose metabolism) and silencing of ptnABCD (the main glucose transporter) and ldh (regulator/enzyme of downstream carbon metabolism). Overall, our study shows how metabolic network architecture and historical contingency serve to predict targets of selection and inform the functional interpretation of evolved mutations. The elucidation of the relationship between molecular constraints and phenotypic trade-offs contributes to an integrative understanding of evolutionary specialization and diversification.
Heterogeneous data integration methods for patient similarity networks

Abstract Patient similarity networks (PSNs), where patients are represented as nodes and their similarities as weighted edges, are being increasingly used in clinical research. These networks provide an insightful summary of the relationships among patients and can be exploited by inductive or transductive learning algorithms for the prediction of patient outcome, phenotype and disease risk. PSNs can also be easily visualized, thus offering a natural way to inspect complex heterogeneous patient data and providing some level of explainability of the predictions obtained by machine learning algorithms. The advent of high-throughput technologies, enabling us to acquire high-dimensional views of the same patients (e.g. omics data, laboratory data, imaging data), calls for the development of data fusion techniques for PSNs in order to leverage this rich heterogeneous information. In this article, we review existing methods for integrating multiple biomedical data views to construct PSNs, together with the different patient similarity measures that have been proposed. We also review methods that have appeared in the machine learning literature but have not yet been applied to PSNs, thus providing a resource to navigate the vast machine learning literature existing on this topic. In particular, we focus on methods that could be used to integrate very heterogeneous datasets, including multi-omics data as well as data derived from clinical information and medical imaging.
Antiretroviral therapy restores the homeostatic state of microglia in SIV‐infected rhesus macaques

Microglia and macrophages are essential for homeostatic maintenance and innate immune response in the brain. They are the first line of defense against infections such as HIV/SIV in the brain. However, they are susceptible to infection and function as viral reservoirs even under effective viral suppression. While current antiretroviral regimens successfully suppress viremia and improve quality of life and lifespan, neurologic complications persist and are in part attributed to activated microglia. We sought to test the hypothesis that brain microglia return to a more homeostatic‐like state when viremia is suppressed by combination antiretroviral therapy. Using the SIV‐rhesus macaque model, we combined single‐cell RNA sequencing, bioinformatics, and pathway analysis to compare gene expression profiles of brain myeloid cells under 4 conditions: uninfected, SIV infected, SIV infected with cART suppression, and SIV encephalitis (SIVE). Our study reveals greater myeloid diversity and an elevated proinflammatory state are associated with untreated SIV infection compared with uninfected animals. The development of encephalitis and suppression of viremia both reduced myeloid diversity. However, they had converse effects on the activation state of microglia and inflammation. Notably, suggestive of a restoration of a homeostatic state in microglia, gene expression and activation of pathways related to inflammation and immune response in cART‐suppressed monkeys were most similar to that in uninfected monkeys. Untreated SIV infection shared characteristics, especially in brain macrophages to SIVE, with SIVE showing dramatic inflammation. In support of our hypothesis, our study demonstrates that cART indeed restores this key component of the brain's homeostatic state.
B Cells and Ligand Activity Is Detectable in EBI2 Guides Serial Movements of Activated

EBV-induced gene 2 (EBI2) was recently shown to direct the delayed movement of activated B cells to interfollicular and outer follicular regions of secondary lymphoid organs and to be required for mounting a normal T-dependent Ab response. In this study, we show that EBI2 promotes an early wave of Ag-activated B cell migration to the outer follicle in mice. Later, when B cells have moved to the T zone in a CCR7-dependent manner, EBI2 helps distribute the cells along the B zone–T zone boundary. Subsequent EBI2-dependent movement to the outer follicle coincides with CCR7 downregulation and is promoted by CD40 engagement. Using a bioassay, we identify a proteinase K-resistant, hydrophobic EBI2 ligand activity in lymphoid and nonlymphoid tissues. Production of EBI2 ligand activity by a cell line is sensitive to statins, suggesting production in a 3-hydroxy-3-methyl-glutaryl-CoA reductase–dependent manner. CD40-activated B cells show sustained EBI2-dependent responsiveness to the bioactivity. These ﬁndings establish a role for EBI2 in helping control B cell position at multiple stages during the Ab response and they suggest that EBI2 responds to a broadly distributed lipid ligand. The Journal of Immunology , 2011, 187: 000–000. cells migrate into a CXCR5-depen-dent manner, the CXCR5 ligand CXCL13, is abundantly stromal cells follicle an m m/min walk,” for
FISH review

J. Meyer, C. Rolf, C. Schiller, S. Rohs, N. Spelten, A. Afchine, M. Zöger, N. Sitnikov, T. D. Thornberry, A. W. Rollins, Z. Bozóki, D. Tátrai, V. Ebert, B. Kühnreich, P. Mackrodt, O. Möhler, H. Saathoff, K. H. Rosenlof, and M. Krämer Institut für Energie und Klimaforschung 7, Forschungszentrum Jülich, 52425 Jülich, Germany Institut für Energie und Klimaforschung 8, Forschungszentrum Jülich, 52425 Jülich, Germany Deutsches Zentrum für Luft und Raumfahrt, FX, 82234 Oberpfaffenhofen, Germany Central Aerological Observatory, Dolgoprudny, Russia NOAA ESRL Chemical Sciences Division, Boulder, USA Cooperative Institute for Research in Environmental Sciences, University of Colorado Boulder, Boulder, CO, USA Department of Optics and Quantum Electronics, University of Szeged, Szeged, Hungary MTA-SZTE Research Group on Photoacoustic Spectroscopy, Szeged, Hungary Physikalisch-Technische Bundesanstalt (PTB), 38116 Braunschweig, Germany
Performance and nutrient utilisation of dairy cows offered from three successive harvests of either a red clover–perennial ryegrass sward or a perennial ryegrass sward

The need to reduce reliance on imported protein feeds within the UK and Ireland has stimulated interest in locally grown forage legume crops, including red clover (Trifolium pratense L. ) . This 13-wk study examined the performance of 28 dairy cows offered silages produced from three successive harvests (H) of either a pure grass sward (GS) receiving 315 kg N/ha per annum or a red clover–perennial ryegrass sward (RCGS) receiving 22 kg N/ha per annum. The crops of H1, H2 and H3 were wilted for 48, 72 and 72 h, respectively. Silages from H1, H2 and H3 were offered for 5, 5 and 3 wk, respectively, with cows supplemented with 8.0 kg concentrate/d throughout the experiment. Digestibility of DM and the effectively degradable protein content were lower, while protein degradability was higher, for RCGS than for GS. Silage DM intakes (DMIs) were higher for RCGS than for GS at H1 and H2, with no differences at H3. Milk yield was higher with RCGS than with GS at H3, with no differences at H1 and H2. Milk fat and milk protein contents were lower with RCGS than with GS at H3 but did not differ at H1 and H2. Faecal N/N intake was higher in the RCGS group than in the GS group at H1, with no differences at H2 and H3. Gross energy digestibility was lower for RCGS than for GS at H2. Although cow performance was higher with RCGS treatment, the responses were variable between harvests, largely reflecting the changing proportion of RC in the swards as the season progressed.