Miracle-dz/UniFAR-FTUs huggingface dataset:
Evolution of the Dietary Patterns across Nutrition Transition in the Sardinian Longevity Blue Zone and Association with Health Indicators in the Oldest Old

Health and longevity in humans are influenced by numerous factors, including lifestyle and nutrition. However, the direct relationship between lifetime eating habits and functional capacity of the elderly is poorly understood. This study investigated the dietary changes across nutrition transition (NT) in the early 1960s, in a population located in the Sardinia island known for its longevity, dubbed as “Longevity Blue Zone” (LBZ), as well as the relationship between the dietary patterns and a panel of health indicators. A total of 150 oldest-old participants (89 women and 61 men, age range 90–101 years) living in the LBZ were recruited. Participants were interviewed using validated questionnaires to assess the consumption frequency of common food items, as well as the correlation with self-rated health, comorbidity, affective and cognitive level, physical mobility, disability and anthropometric parameters. Differences between subgroups were evaluated using the Mann-Whitney U test for independent samples or the Wilcoxon signed-rank test for paired samples. Correlation analysis was performed by calculating the Spearman correlation coefficient, separately in males and females. Compared to the pre-NT epoch, the consumption of meat, olive oil and fresh fruit slightly increased, while the consumption of lard, legumes and vegetables decreased. A significant association was found between increased olive oil intake across NT and self-rated health (ρ = 0.519), mobility (ρ = 0.502), improved vision (ρ = −0.227) and hearing (ρ = −0.314); increased chicken meat intake and performance in activities of daily living (basic activities of daily living: ρ = 0.351; instrumental activities of daily living: ρ = 0.333). Instead, vegetable consumption showed low correlation with health indicators. A mild increase in meat intake, mostly pastured poultry, is associated with better physical performance in the Sardinian LBZ elders, suggesting that a supply of protein may have been crucial to maintaining adequate functional capacity.
Host Cell Restriction Factors of Bunyaviruses and Viral Countermeasures

The Bunyavirales order comprises more than 500 viruses (generally defined as bunyaviruses) classified into 12 families. Some of these are highly pathogenic viruses infecting different hosts, including humans, mammals, reptiles, arthropods, birds, and/or plants. Host cell sensing of infection activates the innate immune system that aims at inhibiting viral replication and propagation. Upon recognition of pathogen-associated molecular patterns (PAMPs) by cellular pattern recognition receptors (PRRs), numerous signaling cascades are activated, leading to the production of interferons (IFNs). IFNs act in an autocrine and paracrine manner to establish an antiviral state by inducing the expression of hundreds of IFN-stimulated genes (ISGs). Some of these ISGs are known to restrict bunyavirus infection. Along with other constitutively expressed host cellular factors with antiviral activity, these proteins (hereafter referred to as “restriction factors”) target different steps of the viral cycle, including viral entry, genome transcription and replication, and virion egress. In reaction to this, bunyaviruses have developed strategies to circumvent this antiviral response, by avoiding cellular recognition of PAMPs, inhibiting IFN production or interfering with the IFN-mediated response. Herein, we review the current knowledge on host cellular factors that were shown to restrict infections by bunyaviruses. Moreover, we focus on the strategies developed by bunyaviruses in order to escape the antiviral state developed by the infected cells.
Effects of Different Types of Physical Activity on Health-Related Quality-of-Life in Korean Women with Depressive Disorder

Depressive disorder is a frequent psychological illness and causes community health problems for many women. It was found that the health-related quality of life (HRQoL) score of many women was altered due to mental and physical problems. Physical activity (PA) might effectively improve the responses of the HRQoL of women with depressive disorder. Therefore, the study aimed to identify the effects of different types of PA (e.g., walking, strength exercise, flexibility exercise) on the responses of the HRQoL of Korean women with depressive disorder. A sample of 1315 Korean women aged 19 or older with a depressive disorder was accumulated. The Korea National Health and Nutrition Examination Survey (KNHANES) and Euro Quality of Life-5 Dimensions (EQ-5D index score) were used. The characteristics of the participants were analyzed by the complex sample in frequency analysis. Furthermore, the complex sample general linear model was used to determine the effects of different types of PA on the HRQoL of Korean women with depressive disorder. In the results, there was a statistically significant difference between the groups who participated for one to two days, who participated for three to four days, and who did not participate in walking at all. In the flexibility exercise, there was a statistically significant difference in the group who participated for three to four days from the group who did not participate at all. However, there was no statistically significant difference in the strength exercise. In conclusion, the walking and flexibility exercises were effective physical activities (PAs) to improve the responses to the HRQoL of Korean women with depressive disorder.
Blood Platelets as an Important but Underrated Circulating Source of TGFβ

When treating diseases related primarily to tissue remodeling and fibrosis, it is desirable to regulate TGFβ concentration and modulate its biological effects. The highest cellular concentrations of TGFβ are found in platelets, with about 40% of all TGFβ found in peripheral blood plasma being secreted by them. Therefore, an understanding of the mechanisms of TGFβ secretion from platelets may be of key importance for medicine. Unfortunately, despite the finding that platelets are an important regulator of TGFβ levels, little research has been carried out into the development of platelet-directed therapies that might modulate the TGFβ-dependent processes. Nevertheless, there are some very encouraging reports suggesting that platelet TGFβ may be specifically involved in cardiovascular diseases, liver fibrosis, tumour metastasis, cerebral malaria and in the regulation of inflammatory cell functions. The purpose of this review is to briefly summarize these few, extremely encouraging reports to indicate the state of current knowledge in this topic. It also attempts to better characterize the influence of TGFβ on platelet activation and reactivity, and its shaping of the roles of blood platelets in haemostasis and thrombosis.
Parents’ willingness to pay for a COVID-19 vaccine for themselves and their children in the United States

ABSTRACT Differences in obtaining a vaccine vary based on a multitude of factors including perceptions of vaccine safety, efficacy and willingness to pay (WTP). This study focuses on parent perceptions toward a vaccine for COVID-19 including their WTP decisions for their children and themselves. A mixed methods design using a cross-sectional survey was used to assess the perceptions of US parents, with children under 18, in response to the COVID-19 pandemic. The survey was administered online in November 2020 and 584 final responses were collected. The questionnaire consisted of approximately 37 closed and open-ended questions regarding personal background information; awareness, knowledge, and experience of the virus; perception toward vaccines and COVID-19; and the contingent valuation of a hypothetical COVID-19 vaccine. WTP was found to be higher for children and a direct relationship between the number of children and WTP was observed. Parents were willing to pay US$228–$291 for a vaccine for themselves and US$243–US$321 for their children. A positive impact on WTP was found with self-reported parent health status but not children. The findings of this study have important implications for policy programs which require detailed cost-benefit analyses. WTP for a COVID-19 vaccine is crucial to determine the partial benefits of vaccinating to reduce the risk of repetitive widespread outbreaks.
Description of a new species of the genus Uropsilus (Eulipotyphla: Talpidae: Uropsilinae) from the Dabie Mountains, Anhui, Eastern China

During a terrestrial vertebrate survey of the Dabie Mountains in Anhui Province, eastern China, we collected four Asian shrew mole specimens (hereafter, shrew moles). Based on published literature and comparison with previously collected materials, the four specimens were similar to shrew moles from the mountains of Southwest China; however, no species in this group has been previously recorded from the Dabie Mountains. The genetic and morphological characteristics of the specimens were analyzed, based upon which a new species of shrew mole is described, named Uropsilus dabieshanensis sp. nov.
Thrombocytopenia and clear corneal incision cataract surgery

Clear corneal incision cataract surgery with pupillary expansion devices and local anesthetic injections can be safely performed in patients with thrombocytopenia. Purpose: To investigate complications and outcomes of clear corneal incision cataract surgery in patients with thrombocytopenia. Setting: 1 veterans hospital and 2 academic medical centers. Design: Multicenter retrospective chart review. Methods: All eyes of thrombocytopenic patients that underwent clear corneal incision cataract surgery with a platelet count of 100 × 103/μL or less measured within 30 days prior to surgery were included. Subject demographics, intraoperative complications, use of pupillary expansion devices, use of local anesthetic injections, and change in corrected distance visual acuity were recorded. Results: 3 sites recorded 40 113 clear corneal incision cataract surgeries, of which 196 eyes (0.49%) of 150 thrombocytopenic patients were recorded. The mean platelet count in the study subjects was 73.0 ± 20.5 × 103/μL. Two cases of intraoperative iris hemorrhage that were readily and controlled occurred in conjunction with pupillary expansion. There were no bleeding complications associated with retrobulbar, peribulbar, or sub-Tenon anesthetic injections. There was a statistically significant improvement (P < .0001) in visual acuity post-operatively. Conclusions: Clear corneal incision cataract surgery with pupillary expansion devices and local anesthetic injections can be safely performed in patients with thrombocytopenia.
Corticosteroids May Have Negative Effects on the Management of Patients with Severe Fever with Thrombocytopenia Syndrome: A Case–Control Study

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral hemorrhagic fever in China, Korea, and Japan. To date, no standardized treatment protocol for SFTS has been established. Corticosteroids (CS) may be administered to patients with SFTS and hemophagocytic syndrome, but its effectiveness and safety are still debatable. We conducted a retrospective case series review at four medical facilities in Miyazaki, Japan. Based on the medical records, clinical data, including the patients background, symptoms, physical findings, laboratory data at initial presentation, treatment, and outcome, were compared between the CS-treated and the non-CS-treated group. A total of 47 patients with confirmed SFTS in each hospital were enrolled in this study; there were 14 fatal cases and 33 nonfatal cases. The case fatality ratio was 29.8%. After adjusting patients’ background by propensity score matching, the case fatality ratio was higher (p = 0.04) and complications of secondary infections, including invasive pulmonary aspergillosis, tended to be more frequent (p = 0.07) in the CS-treated group than in the non-CS-treated group. These data suggested that administration of CS to patients with SFTS should be carefully considered.
Diabesity Associates with Poor COVID-19 Outcomes among Hospitalized Patients

Background Although numerous studies have investigated obesity’s negative effect on coronavirus disease 2019 (COVID-19) outcomes, only a limited number focused on this association in diabetic patients. In this study, we analyzed the association between obesity and COVID-19 outcome (death, intensive care unit [ICU] admission, mechanical ventilation needs, quick Sequential Organ Failure Assessment [qSOFA] score, and confusion, urea, respiratory rate, blood pressure [CURB-65] scores) for hospitalized diabetic patients. Methods In this prospective hospital-based registry of patients with COVID-19 in East Azerbaijan, Iran, 368 consecutive diabetic patients with COVID-19 were followed from admission until discharge or death. Self-reported weight and height were used to calculate body mass index (kg/m2) upon admission. Our primary endpoint was analyzing obesity and COVID-19 mortality association. Assessing the associations among obesity and disease severity, ICU admission, and mechanical ventilation was our secondary endpoint. Results We analyzed data from 317 patients and found no significant difference between obese and non-obese patients regarding frequency of death, invasive mechanical ventilation, ICU admission, CURB-65, or qSOFA scores (P>0.05). After adjusting for confounding factors, obese diabetic COVID-19 patients were 2.72 times more likely to die than non-obese patients. Moreover, ventilator dependence (adjusted odds ratio [aOR], 1.87; 95% confidence interval [CI], 1.03–4.76) and ICU admission (aOR, 2.41; 95% CI, 1.11–5.68) odds were significantly higher for obese patients than non-obese patients. Conclusion The results of the present study indicated that obesity worsens health outcomes for diabetic COVID-19 patients.
Hepatitis C Virus Uses Host Lipids to Its Own Advantage

Lipids and lipoproteins constitute indispensable components for living not only for humans. In the case of hepatitis C virus (HCV), the option of using the products of our lipid metabolism is “to be, or not to be”. On the other hand, HCV infection, which is the main cause of chronic hepatitis, cirrhosis and hepatocellular carcinoma, exerts a profound influence on lipid and lipoprotein metabolism of the host. The consequences of this alternation are frequently observed as hypolipidemia and hepatic steatosis in chronic hepatitis C (CHC) patients. The clinical relevance of these changes reflects the fact that lipids and lipoprotein play a crucial role in all steps of the life cycle of HCV. The virus circulates in the bloodstream as a highly lipidated lipo-viral particle (LVP) that defines HCV hepatotropism. Thus, strict relationships between lipids/lipoproteins and HCV are indispensable for the mechanism of viral entry into hepatocytes, viral replication, viral particles assembly and secretion. The purpose of this review is to summarize the tricks thanks to which HCV utilizes host lipid metabolism to its own advantage.
Multivariate Analysis of Morpho-Physiological Traits Reveals Differential Drought Tolerance Potential of Bread Wheat Genotypes at the Seedling Stage

Drought is one of the foremost environmental stresses that can severely limit crop growth and productivity by disrupting various physiological processes. In this study, the drought tolerance potential of 127 diverse bread wheat genotypes was evaluated by imposing polyethylene glycol (PEG)-induced drought followed by multivariate analysis of several growth-related attributes. Results showed significant variations in the mean values of different morpho-physiological traits due to PEG-induced drought effects. Correlation analysis revealed that most of the studied traits were significantly correlated among them. The robust hierarchical co-clustering indicated that all the genotypes were clustered into four major groups, with cluster 4 (26 genotypes) being, in general, drought-tolerant followed by cluster 1 (19 genotypes) whereas, cluster 2 (55 genotypes) and 3 (27 genotypes) being drought-sensitive. Linear discriminant analysis (LDA) confirmed that around 90% of the genotypes were correctly assigned to clusters. Squared distance (D2) analysis indicated that the clusters differed significantly from each other. Principal component analysis (PCA) and genotype by trait biplot analysis showed that the first three components accounted for 71.6% of the total variation, with principal component (PC) 1 accounting for 35.4%, PC2 for 24.6% and PC3 for 11.6% of the total variation. Both PCA and LDA revealed that dry weights, tissue water content, cell membrane stability, leaf relative water content, root-shoot weight ratio and seedling vigor index played the most important discriminatory roles in explaining drought tolerance variations among 127 wheat genotypes. Our results conclude that the drought-tolerant and -sensitive wheat genotypes identified in this study would offer valuable genetic tools for further improvement of wheat productivity in arid and semi-arid regions during this time of unpredictable climate change.
Acute Pulmonary Histoplasmosis Following COVID-19: Novel Laboratorial Methods Aiding Diagnosis

The acute form of histoplasmosis usually occurs after the exposition of more than one individual to a common environmental source harboring Histoplasma capsulatum. Here, we present two cases of acute pulmonary histoplasmosis seen within two weeks at a reference center for infectious diseases at Rio de Janeiro, Brazil. The patients did not present a common epidemiologic history for histoplasmosis, however both presented COVID-19 before the onset of histoplasmosis symptoms. Due to the difficulties in the diagnosis of acute histoplasmosis, novel laboratory methods such as Western Blot and PCR were included in the investigation of these cases. Both patients presented negative cultures for H. capsulatum and negative urinary galactomannan. However, they presented H and M bands in the Western blot as well as a positive H. capsulatum DNA detection in sputum. These results were available approximately 36 h after sample collection, fastening the beginning of treatment of one patient. Both patients progressed well with itraconazole treatment. These cases suggest that COVID-19 may facilitate the development of acute pulmonary histoplasmosis and, therefore, clinicians must be aware of this differential diagnosis in patients from endemic areas with fever and coughing after recovery from COVID-19.
The Role of Knockout Olfactory Receptor Genes in Odor Discrimination

To date, little is known about the role of olfactory receptor (OR) genes on smell performance. Thanks to the availability of whole-genome sequencing data of 802 samples, we identified 41 knockout (KO) OR genes (i.e., carriers of Loss of Function variants) and evaluated their effect on odor discrimination in 218 Italian individuals through recursive partitioning analysis. Furthermore, we checked the expression of these genes in human and mouse tissues using publicly available data and the presence of organ-related diseases in human KO (HKO) individuals for OR expressed in non-olfactory tissues (Fisher test). The recursive partitioning analysis showed that age and the high number (burden) of OR-KO genes impact the worsening of odor discrimination (p-value < 0.05). Human expression data showed that 33/41 OR genes are expressed in the olfactory system (OS) and 27 in other tissues. Sixty putative mouse homologs of the 41 humans ORs have been identified, 58 of which are expressed in the OS and 37 in other tissues. No association between OR-KO individuals and pathologies has been detected. In conclusion, our work highlights the role of the burden of OR-KO genes in worse odor discrimination.
Comparative studies of callus induction and plant regeneration from mature embryos in rice mutants

Experiments were performed with mature embryos of four rice mutants for in vitro callus induction and plant regeneration. Different concentrations of 2,4-D and BAP combined, Kn and NAA combined and MET were used to assess their effects on induction of callus, shoot and roots. Given concentrations of 2,4-D with BAP and rice mutants in vitro cultures showed that Y-1281 and Atomita-4 had produced the highest percentages of callus at 2 mgl -1 2,4-D with 0.5 mgl -1 BAP in both light and dark conditions. Similar results were obtained in terms of concentrations and genotypes when 2,4-D and BAP were used together. Combined 2,4-D and BAP treatments showed significantly higher callusing under light conditions (90%). For shoot and root regenerations, once again the mutant genotype Y-1281 produced the highest percentages of shoots and roots and it also showed highest root length. And 11 mgl -1 2,4-D, 0.5 mgl -1 NAA combined and 2.5 mgl -1 MET concentration resulted significantly the highest shoot and root regeneration. Keywords: Callus induction; Mature embryos; Plant regeneration; Root induction; Rice DOI: 10.3329/jbau.v7i1. 4796 J. Bangladesh Agril. Univ. 7(1): 39-45, 2009
High Variability in Pre-Oviposition Time Independent of Diet Available at Eclosion: A key Reproductive Trait in the Ladybird Beetle Harmonia axyridis (Coleoptera: Coccinellidae) in Its Native Range

Simple Summary Insect predators need high-quality food to reach sexual maturity and reproduce. Nonetheless, just emerged adults may face starvation when prey are missing, notably in agroecosystems. This study conducted in laboratory conditions explores whether the type of diet (flower food, prey food or no food) available just after adult eclosion is of critical importance for the future fecundity of adult ladybird beetles. Diet readily available just after emergence did not affect the fecundity and egg viability of Harmonia axyridis. Time from eclosion to first egg laying (called pre-oviposition time) of H. axyridis was highly variable and negatively related to fecundity and egg viability. This study clarifies that the food readily available at adult eclosion does not affect the capacity of H. axyridis to reproduce, provided that adults find prey within a few days. It is concluded that the reproductive strategy of H. axyridis allows this ladybird beetle species to be highly adapted to heterogenous environments in its native range, highlighting the importance of diversifying agroecosystems to enhance biological regulation of pests. Abstract While insect predators need high-quality food to reach sexual maturity and reproduce, starvation following adult eclosion may occur when prey are missing in agroecosystems. This study explores whether the type of diet available at eclosion determines the future fecundity of newly emerged adult predators. In a laboratory experiment, three different diets (i.e., flowers of Perilla frutescens (L.) Britton, eggs of Ephestia kuehniella Zeller as prey, or no food) were offered to adult females of the ladybird beetle Harmonia axyridis Pallas during their first three days after adult eclosion. On the fourth day, each female was paired with a prey-fed male and the pair was subsequently fed with prey. Diet at eclosion did not affect pre-oviposition time, the number of eggs oviposited daily, or the viability of egg batches. High variability in pre-oviposition time among females was observed for all diets. Significant negative linear relationships were found between pre-oviposition time and both the number of eggs oviposited daily and the viability of egg batches. This study clarifies that the food readily available at adult eclosion does not affect the capacity of H. axyridis to reproduce, provided that adults find prey within a few days. More generally, it shows that the reproductive traits of H. axyridis allow this generalist predator to be highly adapted to heterogenous environments in its native range. It is concluded that the variety of habitats offered by diversified agroecosystems may highly benefit the ladybird beetle H. axyridis, and potentially enhance its ability to biologically regulate crop pests.