Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in Serum Progesterone, Estradiol-17β, Luteinizing Hormone and Prolactin in Lactating and Non-lactating Japanese Black Bears (Ursus thibetanus japonicus)

The concentrations of progesterone (P4), estradiol-17 β (E2), luteinizing hormone (LH) and prolactin (PRL) in serum obtained from 9 captive female Japanese black bears, Ursus thibetanus japonicus, (7 lactating and 2 non-lactating) were obtained by a heterologous radioimmunoassay to clarify hormonal profiles during the pre- and post-parturition and lactation periods. The present study provides the first evidence to validate LH and PRL radioimmunoassay in bear sera by using the canine LH and PRL and the antibody to these hormones. In 7 bears which gave birth and lactated, serum P4 concentrations were high during prepartum phases in December and January but fell rapidly after parturition in February (p<0.05; comparison of pre- and post-parturient phases). Serum E2 and LH concentrations did not change significantly throughout pre- and post-parturition. Serum PRL concentrations increased significantly after parturition in February (p<0.05; comparison of pre- and post-parturient phases) and exceeded 10 ng/ml in all lactating bears from Days 41~60 after parturition in March. On the other hand, in 2 bears which did not produce cubs, serum P4 concentrations were high in December and January and decreased after February. Serum E2 concentrations were relatively low between December and February but increased in March. Serum LH concentrations were relatively high in December and remained low throughout January to April, whereas PRL concentrations increased gradually after February, but were below 10 ng/ml. These results suggest that a rapid decline in P4 and a marked increase in PRL occurred in association with events such as parturition and lactation, but there is no clear evidence that changes in serum E2 and LH concentrations are associated with parturition and lactation.
SARS-CoV-2 proteins exploit host’s genetic and epigenetic mediators for the annexation of key host signaling pathways that confers its immune evasion and disease pathophysiology

The constant rise of the death toll and cases of COVID-19 has made this pandemic a serious threat to human civilization. Understanding of host-SARS-CoV-2 interaction in viral pathogenesis is still in its infancy. In this study we aimed to correlate how SARS-CoV-2 utilizes its proteins for tackling the host immune response; parallelly, how host epigenetic factors might play a role in this pathogenesis was also investigated. We have utilized a blend of computational and knowledgebase approach to elucidate the interplay between host and SARS-CoV-2. Integrating the experimentally validated host interactome proteins and differentially expressed host genes due to SARS-CoV-2 infection, we have taken a blend of computational and knowledgebase approach to delineate the interplay between host and SARS-CoV-2 in various signaling pathways. Also, we have shown how host epigenetic factors are involved in the deregulation of gene expression. Strikingly, we have found that several transcription factors and other epigenetic factors can modulate some immune signaling pathways, helping both host and virus. We have identified miRNA hsa-miR-429 whose transcription factor was also upregulated and targets were downregulated and this miRNA can have pivotal role in suppression of host immune responses. While searching for the pathways in which viral proteins interact with host proteins, we have found pathways like-HIF-1 signaling, autophagy, RIG-I signaling, Toll-like receptor signaling, Fatty acid oxidation/degradation, Il-17 signaling etc significantly associated. We observed that these pathways can be either hijacked or suppressed by the viral proteins, leading to the improved viral survival and life-cycle. Moreover, pathways like-Relaxin signaling in lungs suggests aberration by the viral proteins might lead to the lung pathophysiology found in COVID-19. Also, enrichment analyses suggest that deregulated genes in SARS-CoV-2 infection are involved in heart development, kidney development, AGE-RAGE signaling pathway in diabetic complications etc. might suggest why patients with comorbidities are becoming more prone to SARS-CoV-2 infection. Our results suggest that SARS-CoV-2 integrates its proteins in different immune signaling pathway and other cellular signaling pathways for developing efficient immune evasion mechanisms, while leading the host to more complicated disease condition. Our findings would help in designing more targeted therapeutic interventions against SARS-CoV-2.
The Characteristics of Patients With Possible Transient Ischemic Attack and Minor Stroke in the Hunter and Manning Valley Regions, Australia (the INSIST Study)

Background: Transient ischemic attack (TIA) and minor stroke (TIAMS) are risk factors for stroke recurrence. Some TIAMS may be preventable by appropriate primary prevention. We aimed to recruit “possible-TIAMS” patients in the INternational comparison of Systems of care and patient outcomes In minor Stroke and TIA (INSIST) study. Methods: A prospective inception cohort study performed across 16 Hunter–Manning region, Australia, general practices in the catchment of one secondary-care acute neurovascular clinic. Possible-TIAMS patients were recruited from August 2012 to August 2016. We describe the baseline demographics, risk factors and pre-event medications of participating patients. Results: There were 613 participants (mean age; 69 ± 12 years, 335 women), and 604 (99%) were Caucasian. Hypertension was the most common risk factor (69%) followed by hyperlipidemia (52%), diabetes mellitus (17%), atrial fibrillation (AF) (17%), prior TIA (13%) or stroke (10%). Eighty-nine (36%) of the 249 participants taking antiplatelet therapy had no known history of cardiovascular morbidity. Of 102 participants with known AF, 91 (89%) had a CHA2DS2-VASc score ≥ 2 but only 47 (46%) were taking anticoagulation therapy. Among 304 participants taking an antiplatelet or anticoagulant agent, 30 (10%) had stopped taking these in the month prior to the index event. Conclusion: This study provides the first contemporary data on TIAMS or TIAMS-mimics in Australia. Community and health provider education is required to address the under-use of anticoagulation therapy in patients with known AF, possibly inappropriate use of antiplatelet therapy and possibly inappropriate discontinuation of antiplatelet or anticoagulation therapy.
A comparison of two methods for estimating measurement repeatability in morphometric studies

Measurement repeatability is often reported in morphometric studies as an index of the contribution of measurement error to trait measurements. However, the common method of remeasuring a mounted specimen fails to capture some components of measurement error, and could therefore yield inflated repeatability estimates. Remounting specimens between successive measurements is likely to provide more realistic estimates of repeatability, particularly for small structures that are difficult to measure. Using measurements of 22 somatic and genitalic traits of the neriid fly Telostylinus angusticollis, we compared repeatability estimates obtained via remeasuring (where a mounted specimen is measured twice) versus remounting (where a specimen is remounted between measurements). We also asked whether the difference in repeatability estimates obtained via the two methods depends on trait size. Repeatability estimates obtained via remounting were lower than estimates obtained via remeasuring for each of the 22 traits, and the difference between estimates obtained via the two methods was generally greater for small structures (genitalic traits) than for large structures (legs, wings). Remounting specimens between successive measurements can provide more accurate estimates of measurement repeatability than remeasuring from a single mount, especially for small structures that are difficult to measure.
Yield, N uptake, and apparent N-use efficiency of winter wheat and winter barley grown in different cropping systems

Increasing the efficiency with which crops use supplied nitrogen (N) can minimize the impact on the environment. In the growing seasons 1990/91 to 1992/93, the effects of different cropping systems on yield, N uptake by the grain and apparent N-use efficiency (NUE) of the grain of winter wheat and winter barley were investigated in a factorial field experiment at Hohenschulen Experimental Station near Kiel in NW Germany. The crop rotation was oilseed rape–winter wheat–winter barley, and soil tillage (conservation tillage without ploughing, conventional tillage), application of pig slurry (none, autumn, spring, autumn+spring), mineral N fertilization (0–240 kg N ha−1) and application of fungicides (none, applications against pathogens of the stems, leaves and ears) were all varied. Each year, the treatments were applied to all three crops of the rotation and were located on the same plots. Averaged over all factors, wheat yield was >7 t ha−1 dry matter in all years and N uptake of the harvested grain varied between 140 and 168 kg N ha−1. Pig slurry application in autumn increased grain yield and N uptake more than spring slurry in two out of three years. Mineral N unfertilized wheat yielded only 5·3–6·3 t ha−1 depending on the year, mineral N fertilization increased wheat yield up to 8 t ha−1. Barley yield was lower than wheat yield, ranging from 4·5 t ha−1 in 1993 to 6·3 t ha−1 in 1992. Unlike wheat, spring slurry N affected barley yield and N uptake more than autumn slurry. Wheat apparently utilized 12–21% and barley up to 13% of the applied slurry N for its grain development. In 1991, the highest apparent slurry N-use efficiency (SNUE) of wheat and barley occurred after the late spring slurry application. However, in the following years, autumn SNUE of wheat was similar to (1992) or higher than (1993) spring SNUE, presumably because of vigorous tiller growth before winter. Additionally applied mineral fertilizer N decreased SNUE. Apparent mineral fertilizer N-use efficiency (FNUE) was higher than SNUE and ranged in wheat from 40 to 59% and in barley between 19 and 37% of the applied mineral fertilizer N. FNUE decreased with increasing N fertilization. To improve the N-use efficiency of both slurry N and mineral fertilizer N, more information is needed about the combined use of both N sources, with special emphasis on split applications of slurry as is common practice for mineral N fertilizer.
Meta-analysis of nutritional effects on conjugated linoleic acid (CLA) in milk fat of dairy cows

Abstract A meta-analysis was carried out on 41 selected studies to obtain more reliable results about the influence of some nutritional factors on conjugated linoleic acid (CLA) in milk fat. Data were analysed with a linear mixed model, including the study as random variable, that highlighted a significant effect on milk CLA content of fat source and the physical form of the lipid supplement used in the diet. The content of fat in the diet and the forage/concentrate ratio seem do not have significant effects.
Individual Differences in Children’s Development of Scientific Reasoning Through Inquiry-Based Instruction: Who Needs Additional Guidance?

Scientific reasoning involves a person’s ability to think and act in ways that help advance their understanding of the natural world. Young children are naturally inclined to engage in scientific reasoning and display an emerging competence in the component skills of, for example, hypothesizing, experimenting and evaluating evidence. Developmental psychology research has shown that same-age children often differ considerably in their proficiency to perform these skills. Part of this variation comes from individual differences in cognition; another part is due to the fact that the component skills of scientific reasoning emerge at a different age and mature at a different pace. Significantly less attention has been paid to children’s capacity to improve in scientific reasoning through instruction and deliberate practice. Although elementary science lessons are generally effective to raise the skill level of a group of learners, not all children benefit equally from the instructional treatment they receive. Knowing what causes this differential effectiveness is important as it can inform the design of adaptive instruction and support. The present study therefore aimed to identify and explain how fifth-graders (N = 138) improve their scientific reasoning skills over the course of a 5-week inquiry-based physics unit. In line with our expectations, significant progress was observed in children’s achievements on a written scientific reasoning test, which was administered prior to and after the lessons, as well as in their responses to the questions and assignments that appeared on the worksheets they filled out during each lesson. Children’s reading comprehension and mathematical skillfulness explained a portion of the variance in children’s pretest-posttest gain. As these overall results did not apply equally to all component skills of scientific reasoning, we recommend science teachers to adapt their lessons based on children’s past performance in reading and math and their actual performance of each scientific reasoning skill. The orchestration and relative effectiveness of both adaptive science teaching approaches is an interesting topic for future research.
Critical levels of mask efficiency and of mask adoption that theoretically extinguish respiratory virus epidemics

Using a respiratory virus epidemiological model we derive equations for the critical levels of mask efficiency (fraction blocked) and mask adoption (fraction of population wearing masks) that lower the effective reproduction number to unity. The model extends a basic epidemiological model and assumes that a specified fraction of a population dons masks at a given initial number of infections. The model includes a contribution from the ocular (nasolacrimal duct) route, and does not include contributions from contact (fomite) routes. The model accommodates dose-response (probability of infection) functions that are linear or non-linear. Our motivation to study near-population-wide mask wearing arises from the concept that, between two mask wearers, the concentration of particles at inhalation should be the square of the mask penetration fraction. This combination, or team, of masks can provide a strong dose-lowering squaring effect, which enables the use of lower-efficiency, lower-cost, lower pressure-drop (easier breathing) masks. For an epidemic with basic reproduction number R0=2.5 and with a linear dose-response, the critical mask efficiency is calculated to be 0.5 for a mask adoption level of 0.8 of the population. Importantly, this efficiency is well below that of a N95 mask, and well above that of some fabric masks. Numerical solutions of the model at near-critical levels of mask efficiency and mask adoption demonstrate avoidance of epidemics. To be conservative we use mask efficiencies measured with the most-penetrating viral-particle sizes. The critical mask adoption level for surgical masks with an efficiency of 0.58 is computed to be 0.73. With surgical masks (or equally efficient substitutes) and 80% and 90% adoption levels, respiratory epidemics with R0 of about 3 and 4, respectively, would be theoretically extinguished.
Targeting the Dimerization of the Main Protease of Coronaviruses: A Potential Broad-Spectrum Therapeutic Strategy

A new coronavirus (CoV) caused a pandemic named COVID-19, which has become a global health care emergency in the present time. The virus is referred to as SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) and has a genome similar (∼82%) to that of the previously known SARS-CoV (SARS coronavirus). An attractive therapeutic target for CoVs is the main protease (Mpro) or 3-chymotrypsin-like cysteine protease (3CLpro), as this enzyme plays a key role in polyprotein processing and is active in a dimeric form. Further, Mpro is highly conserved among various CoVs, and a mutation in Mpro is often lethal to the virus. Thus, drugs targeting the Mpro enzyme significantly reduce the risk of mutation-mediated drug resistance and display broad-spectrum antiviral activity. The combinatorial design of peptide-based inhibitors targeting the dimerization of SARS-CoV Mpro represents a potential therapeutic strategy. In this regard, we have compiled the literature reports highlighting the effect of mutations and N-terminal deletion of residues of SARS-CoV Mpro on its dimerization and, thus, catalytic activity. We believe that the present review will stimulate research in this less explored yet quite significant area. The effect of the COVID-19 epidemic and the possibility of future CoV outbreaks strongly emphasize the urgent need for the design and development of potent antiviral agents against CoV infections.
Ventricular fibrillation mechanism and global fibrillatory organization are determined by gap junction coupling and fibrosis pattern

Abstract Aims Conflicting data exist supporting differing mechanisms for sustaining ventricular fibrillation (VF), ranging from disorganized multiple-wavelet activation to organized rotational activities (RAs). Abnormal gap junction (GJ) coupling and fibrosis are important in initiation and maintenance of VF. We investigated whether differing ventricular fibrosis patterns and the degree of GJ coupling affected the underlying VF mechanism. Methods and results Optical mapping of 65 Langendorff-perfused rat hearts was performed to study VF mechanisms in control hearts with acute GJ modulation, and separately in three differing chronic ventricular fibrosis models; compact fibrosis (CF), diffuse fibrosis (DiF), and patchy fibrosis (PF). VF dynamics were quantified with phase mapping and frequency dominance index (FDI) analysis, a power ratio of the highest amplitude dominant frequency in the cardiac frequency spectrum. Enhanced GJ coupling with rotigaptide (n = 10) progressively organized fibrillation in a concentration-dependent manner; increasing FDI (0 nM: 0.53 ± 0.04, 80 nM: 0.78 ± 0.03, P < 0.001), increasing RA-sustained VF time (0 nM: 44 ± 6%, 80 nM: 94 ± 2%, P < 0.001), and stabilized RAs (maximum rotations for an RA; 0 nM: 5.4 ± 0.5, 80 nM: 48.2 ± 12.3, P < 0.001). GJ uncoupling with carbenoxolone progressively disorganized VF; the FDI decreased (0 µM: 0.60 ± 0.05, 50 µM: 0.17 ± 0.03, P < 0.001) and RA-sustained VF time decreased (0 µM: 61 ± 9%, 50 µM: 3 ± 2%, P < 0.001). In CF, VF activity was disorganized and the RA-sustained VF time was the lowest (CF: 27 ± 7% vs. PF: 75 ± 5%, P < 0.001). Global fibrillatory organization measured by FDI was highest in PF (PF: 0.67 ± 0.05 vs. CF: 0.33 ± 0.03, P < 0.001). PF harboured the longest duration and most spatially stable RAs (patchy: 1411 ± 266 ms vs. compact: 354 ± 38 ms, P < 0.001). DiF (n = 11) exhibited an intermediately organized VF pattern, sustained by a combination of multiple-wavelets and short-lived RAs. Conclusion The degree of GJ coupling and pattern of fibrosis influences the mechanism sustaining VF. There is a continuous spectrum of organization in VF, ranging between globally organized fibrillation sustained by stable RAs and disorganized, possibly multiple-wavelet driven fibrillation with no RAs.
Pharmacological Augmentation in Unipolar Depression: A Guide to the Guidelines

Abstract Background Pharmacological augmentation is a recommended strategy for patients with treatment-resistant depression. A range of guidelines provide advice on treatment selection, prescription, monitoring and discontinuation, but variation in the content and quality of guidelines may limit the provision of objective, evidence-based care. This is of importance given the side effect burden and poorer long-term outcomes associated with polypharmacy and treatment-resistant depression. This review provides a definitive overview of pharmacological augmentation recommendations by assessing the quality of guidelines for depression and comparing the recommendations made. Methods A systematic literature search identified current treatment guidelines for depression published in English. Guidelines were quality assessed using the Appraisal of Guidelines for Research and Evaluation II tool. Data relating to the prescription of pharmacological augmenters were extracted from those developed with sufficient rigor, and the included recommendations compared. Results Total of 1696 records were identified, 19 guidelines were assessed for quality, and 10 were included. Guidelines differed in their quality, the stage at which augmentation was recommended, the agents included, and the evidence base cited. Lithium and atypical antipsychotics were recommended by all 10, though the specific advice was not consistent. Of the 15 augmenters identified, no others were universally recommended. Conclusions This review provides a comprehensive overview of current pharmacological augmentation recommendations for major depression and will support clinicians in selecting appropriate treatment guidance. Although some variation can be accounted for by date of guideline publication, and limited evidence from clinical trials, there is a clear need for greater consistency across guidelines to ensure patients receive consistent evidence-based care.
Determinants of low uptake of vaccination against influenza, measles, and hepatitis B among healthcare professionals in Greece: a multicenter cross-sectional study

ABSTRACT Vaccination is recommended for healthcare professionals (HCPs) to protect them against vaccine-preventable diseases (VPDs); however, uptake rates are low. This study aimed to evaluate HCPs’ influenza, hepatitis B, and measles vaccine uptake in all healthcare levels in Crete, Greece. We conducted a questionnaire–based, cross-sectional multicenter study in 2018, including HCPs employed at 18 primary care centers and 3 hospitals. Overall, 2,246 HCPs responded (57.2% of the target population). The influenza vaccine uptake rate was 36.1% (810/2,246), with the annual vaccination rate at 14.8% (332/2,246) over the previous 5 years. Concurrently, the hepatitis B 3-dose vaccine uptake rate was 60.3% (1,316/2,181). Among the participating HCPs, 70.7% (1,457/2,061) had measles immunity due to previous illness (959/2,061, 46.5%), a 2-dose vaccination scheme (461/2,061, 22.4%), or serological confirmation (37/2,061, 1.8%). Vaccine uptake rates differed between groups depending on age, profession, and workplace setting. Logistic regression analysis revealed that risk factors for no influenza vaccine uptake during the previous season were younger age (≤45 years; odds ratio [OR] 1.35, 95% confidence interval [CI]: 1.08–1.66), profession other than physician (OR 2.94, 95%CI: 2.09–4.12), and working in hospitals (OR 1.39, 95%CI 1.02–1.89). Older age (>45 years) was an independent risk factor for not receiving a measles (OR 26.74, 95%CI: 17.41–41.06) or hepatitis B vaccine (OR 1.36, 95%CI 1.09–1.7). Working in primary care was an independent risk factor for not getting a hepatitis B vaccine (OR 1.52, 95%CI: 1.15–2.1). Our findings indicate that individualized and targeted interventions should be implemented to increase vaccine uptake among HCPs.