Miracle-dz/UniFAR-FTUs huggingface dataset:
Depression and Suicidal Ideation in Patients with Obsessive Compulsive Disorder

Higher percentages of suicidal ideation and attempt have been reported in relation to OCD patients. Consistent findings as to the role of depression and suicidal ideation have not been reported and thus these phenomena need more research. The aim of the current study was to study the relationship of suicidal ideation, depression with the severity of obsessions and compulsions. 30 patients diagnosed with OCD were checked for the severity of obsessions and compulsions using Yale-Brown Obsessive Compulsive Scale (Y-BOCS), the severity of depression using Hamilton Scale for Depression (HAM-D) and presence of suicidal ideation using Beck’s scale for suicidal ideation (BSIS). Frequency of scores on HAM-D and BSIS were calculated in relation to the severity of scores obtained on Y-BOCS. Pearson product moment correlation was done to assess the relationship between depression and OCD as well as suicidal ideation and OCD. The severity of depression and suicidal ideation varied in accordance with the severity of OCD scores. There was a significant positive correlation between scores on HAM-D and Y-BOCS as well as a significant positive correlation between scores on BSIS and Y-BOCS. hospital setting in Lucknow aimed at assessing suicidal behaviours among OCD patients. Yale-Brown Obsessive Compulsive Scale (Goodman, et al., 1989) was used for assessing the severity of OCD symptoms. Beck’s Scale for Suicidal Ideation (BSIS) (Beck, Kovacs, & Weissman, 1979) and Beck Scale for Hopelessness (BSH) (Beck, Steer, Kovacs, & Garrison, 1985) were used in the study. 3 groups were made dividing the patients among the subclinical group and clinical group based on the severity of scores on Y-BOCS. Around 52% of patients diagnosed with OCD reported a score of more than two on the suicidal ideation scale. The clinical group has significantly higher scores on BSIS and BSH as compared to the subclinical group. Overall, BSIS and BSH scores correlated significantly with Y-BOCS scores (Dhyani, Trivedi, Nischal, Sinha, & Verma, A cross-sectional study was conducted over a period of 1 month in a government hospital setting in Gwalior. 30 patients diagnosed as having OCD according to DSM-V criteria as well as ICD-10 criteria were included in the sample. Patients ranging from 18 years to 50 years of age were included in the sample. Patients with a history of organic mental disorders, substance use, major disorders from the spectrum of psychotic disorders (schizophreniform, schizophrenia, etc.) along with the presence of any other major mental disorders apart from depression were excluded. Patients were given entire information regarding the purpose of the study and informed consent was taken.
USER CENTERED DESIGN IN THE CREATION OF A SURGICAL INTENSIVE CARE LOG by

The Residency Review Committee (RRC) requires that general surgery residents document their Surgical Intensive Care Unit (SICU) experiences. To satisfy these requirements we created a web based intranet log to make it easier for residents to track their patients and determine when these requirements were complete. A premium was put on usability to promote acceptance by surgical residents. A prototype web site was designed with input from an attending general surgeon. Three general surgery residents were selected to participate in the iterative design phase. They went through three iterations using a “think-aloud” method while performing tasks on the prototype web site. Each iteration led to improvements to the web site. In a comparison test, a group of seven medical students performed 14 typical web site tasks using both the prototype and the final versions. They were asked to complete a Questionnaire for User Interaction Satisfaction (QUIS) for each version. The time for completion of these tasks was also recorded. The user interaction satisfaction did not show any improvement (F(1,6)=0.13, p=0.912). Similarly, there was no improvement in times for delete and add tasks ( Delete F(1,5) = 0.949, p=0.375, Add F(1,5)=0.267, p=0.628 ); however, the time to complete edit tasks was faster for the final version of the web site (F (1,5)= 14.3, p=0.013). The primary reason for not detecting other differences between the two web sites is likely that the comparison study did not have sufficient power. This was suggested by the participants whose comments favored the final version over the prototype as well as a trend of consistently higher mean subset scores in the final version. The results indicate that differences may be seen when more complex tasks are completed (editing information) versus the two simpler tasks (adding or deleting a patient record in a web site). Future studies should focus on the impact of navigation strategies on speed and data warehouse approaches to creating the application. This study shows the benefits of using an iterative design approach to create a usable web site and demonstrates the importance of further research in the field of usability.
Nitrate reductase (15)N discrimination in Arabidopsis thaliana,

Stable 15 N isotopes have been used to examine movement of nitrogen (N) through various pools of the global N cycle. A central reaction in the cycle involves the reduction of nitrate (NO − 3 ) to nitrite (NO − 2 ) catalyzed by nitrate reductase (NR). Discrimination against 15 N by NR is a major determinant of isotopic differences among N pools. Here, we measured in vitro 15 N discrimination by several NRs puriﬁed from plants, fungi, and a bacterium to determine the intrinsic 15 N discrimination by the enzyme and to evaluate the validity of measurements made using 15 N-enriched NO − 3 . Observed NR isotope discrimination ranged from 22 to 32 (cid:2) (kinetic isotope effects of 1.022–1.032) among the different isozymes at natural abundance 15 N (0.37%). As the fractional 15 N content of substrate NO − 3 increased from natural abundance, the product 15 N fraction deviated signiﬁcantly from that expected based on substrate enrichment and 15 N discrimination measured at natural abundance. Additionally, isotopic discrimination by denitrifying bacteria used to reduce NO − 3 and NO − 2 in some protocols became a greater source of error as 15 N enrichment increased. We brieﬂy discuss potential causes of the experimental artifacts with enriched 15 N and recommend against the use of highly enriched 15 N tracers to study N discrimination in plants or soils.
Apolipoprotein E-derived peptides block α 7 neuronal nicotinic ACh receptors expressed in Xenopus oocytes

For decades the pathology of Alzheimer’s disease has been associated with dysfunction of cholinergic signaling; however, the cellular mechanisms by which nicotinic acetylcholine receptor (nAChR) function is impaired in Alzheimer’s disease are as yet unknown. The most significant genetic risk factor for the development of Alzheimer’s disease is inheritance of the ε 4 allele of apolipoprotein E (apoE). Recent data has demonstrated the ability of apoE-derived peptides to inhibit nAChRs in rat hippocampus. In the current study, the functional interaction between nAChRs and apoE-derived peptides was investigated in Xenopus oocytes expressing selected nAChRs. Both a 17 amino acid peptide fragment, apoE 133-149 , and an 8 amino acid peptide, apoE 141-148 , were able to maximally block ACh-mediated peak current responses for homomeric α 7 nAChRs. ApoE peptide inhibition was dose-dependent, and voltage- and activity-independent. The current findings suggest that apoE peptides are non-competitive for acetylcholine and do not block functional α -bungarotoxin binding. ApoE peptides had a significantly decreased ability to inhibit ACh-mediated peak current responses for α 4 β 2 and α 2 β 2 nAChRs. Amino acid substitutions in the apoE peptide sequence suggest that the arginines are critical for peptide blockade of the α 7 nAChR. The current data suggests that apoE fragments can disrupt nAChR signaling through a direct blockade of α 7 nAChRs. These results may be useful in elucidating the mechanisms underlying memory loss and cognitive decline seen in Alzheimer’s disease, as well as aid in the development of novel therapeutics using apoE-derived peptides. Recent work has demonstrated that apoE-derived peptides from the LDL receptor binding region inhibit native α 7-containing nAChRs expressed on interneurons in rat hippocampal slices, and that this inhibition was specific for excitatory receptors in the superfamily of ligand-gated ion channels The current study probes the functional interaction between apoE-derived peptides and nAChRs expressed in Xenopus oocytes. The selectivity of apoE peptides for α 7- and non α 7-containing nAChRs was investigated, as well as the sequence specificity for apoE peptide interaction with nAChRs. The nature of the apoE peptide/nAChR interaction was also explored. The current data supports the hypothesis that apoE-derived peptides disrupt cholinergic signaling through a direct blockade of α 7 nAChRs.
MSA Short Course

Mineral particles with diameters on the scale of nanometers (nanoparticles) are important constituents of natural environments. The small size of such particles has a host of consequences for biogeochemical systems, which we will review in this chapter. We begin by briefly reviewing what is known about how and when nanoparticles form and the ways in which nanoparticles impact natural processes. Nanoparticles form via a variety of inorganic and biological pathways and may be introduced into the environment as a consequence of human activity. They are widespread in the environment (Penn et al. 2001, Banfield & Navrotsky 2001, van der Zee et al. 2003, Kennedy et al. 2003a, 2003b), although few quantitative studies of their abundance are available. While all crystals begin as very small particles, an important subset retain small size at the Earth’s surface over relatively long time scales, because the combination of low temperature and low solubility inhibits growth. As a consequence,
The Role of Family Life and the Influence of Peer Pressure on Delinquency: Qualitative Evidence from Malaysia

Juvenile delinquency is always seen as a public health problem which needs intervention at various levels. Identifying which factors may lead juveniles to delinquency is a long-standing question among criminologists. This remains the case in Malaysia. There are studies that have explored the impact of problem-solving skills, low socioeconomic status, and gender differences in predicting the delinquent behavior of youth in Malaysia. However, very few studies have aimed to find an in-depth understanding of the effects of family roles and peer pressure on delinquency in Malaysia. The present qualitative research was designed to fill this gap in the literature. In-depth interviews were conducted with 12 young male prisoners (juvenile delinquents) in Malaysia to explore the influences of family life and peer pressure on delinquency. The current study showed that parental un-involvement, parent separation, peer pressure, criminal gang membership, and parents’ involvement in crime were the important factors for involvement in delinquency. The findings revealed the importance of guidance and counseling for parents and adolescents, to help them cope with life challenges and to build their social and emotional skills, as well as the necessity of appointing school psychologists and public health experts to help the youths become valuable individuals.
The impact of sport organization employees’ justice perceptions on organizational citizenship and dysfunctional behavior: the affect-based model

ABSTRACT This study investigated the impact of sport organization employees’ justice perceptions on organizational citizenship behavior (OCB) and organizational dysfunctional behavior (ODB). Drawing from social exchange and frustration–aggression hypothesis, we presented an affect-based model of employees’ behavioral responses to justice perceptions. We sampled paid staff from NCAA Division I universities’ athletic departments (N = 602) and found that procedural, interpersonal and informational justice relate positively (negatively) to positive (negative) affect, which in turn leads to organizational citizenship (dysfunctional) behavior. Further, exchange orientation moderated the positive relationship between negative affect and ODB, such that the positive impact of employees’ negative affect – caused by organizational injustice – on ODB became stronger as exchange orientation increased. Our findings advance the understanding of organizational justice in sport by illuminating the role of affect and exchange orientation in the relationship between organizational justice and OCB/ODB. Our research also offers sports organizations practical insights into how to effectively manage their employees for eliciting OCB and diminishing ODB. HIGHLIGHTS This study constructs an affect-based model of behavioral responses to justice perception. Procedural, interactional and informational justice were related positively to organizational citizenship behavior via positive affect. Procedural, interactional and informational justice were related negatively to organizational dysfunctional behavior via negative affect. Exchange orientation amplified the positive relationship between negative affect and organizational dysfunctional behavior.
Explore the moderating impact of learners’ anticipated emotions on mobile learning outcome: A moderated mediation model

ABSTRACT The study results have indicated the desire to take mobile learning, attitude towards mobile learning and self-management of learning are positively linked to mobile learning continuance intention. Moreover, it has been found the desire to take mobile learning mediates the link between attitude towards mobile learning and mobile learning continuance intention and positive anticipated emotions reinforce the positive link between attitude towards mobile learning and desire to take mobile learning. Finally, the study findings have shown subjective norm, negative anticipated emotions, positive anticipated emotions and attitude towards mobile learning are positively associated with desire to take mobile learning.
Human PARP1 Facilitates Transcription through a Nucleosome and Histone Displacement by Pol II In Vitro

Human poly(ADP)-ribose polymerase-1 (PARP1) is a global regulator of various cellular processes, from DNA repair to gene expression. The underlying mechanism of PARP1 action during transcription remains unclear. Herein, we have studied the role of human PARP1 during transcription through nucleosomes by RNA polymerase II (Pol II) in vitro. PARP1 strongly facilitates transcription through mononucleosomes by Pol II and displacement of core histones in the presence of NAD+ during transcription, and its NAD+-dependent catalytic activity is essential for this process. Kinetic analysis suggests that PARP1 facilitates formation of “open” complexes containing nucleosomal DNA partially uncoiled from the octamer and allowing Pol II progression along nucleosomal DNA. Anti-cancer drug and PARP1 catalytic inhibitor olaparib strongly represses PARP1-dependent transcription. The data suggest that the negative charge on protein(s) poly(ADP)-ribosylated by PARP1 interact with positively charged DNA-binding surfaces of histones transiently exposed during transcription, facilitating transcription through chromatin and transcription-dependent histone displacement/exchange.
Relationship between Employee’s Satisfaction, Engagement and Turnover Intention

The aim of this paper was to study the relationship between employee satisfaction and engagement towards turnover intention. A total of 61 participants were non-randomly selected through convenience sampling and purposive sampling technique. The study was conducted using quantitative approach where the participants were asked to fill up the questionnaire using the google form link. Instruments used in this study was The Minnesota Satisfaction Questionnaire (MSQ) by Weiss, Dawis, England, and Lofquist (1967), Utrecht Work Engagement Scale (UWES) by Schaufeli and Bakker (2004) and Turnover Intention Scale (TIS-15) by Roodt (2004). The data collected were analysed using the SPSS software. Result of this study indicate that employee satisfaction has a positive effect towards employee’s engagement. Result also indicated there is a negative relationship between employee satisfaction and engagement towards turnover intention of employee. The implication of this study was suitable for companies that are from various industry such as primary, secondary and tertiary industry. Limitation of the study are such as uneven number of participants from age group and different companies cultural background of all participants.
Development and Validation of an UHPLC-MS/MS Method for the Simultaneous Determination of 11 EU-Regulated Mycotoxins in Selected Cereals

The availability of reliable sensitive multi-analyte methods for unambiguous determination of mycotoxins is crucial for ensuring food and feed safety, considering their adverse health effects and (co-)occurrence in various foods. Accordingly, a multi-mycotoxin confirmatory method for simultaneous determination of 11 mycotoxins regulated in cereals within the European Union (EU) using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and in-house validated to fit the EU legislation requirements for analytical methods. A simple sample preparation was based on a solid–liquid extraction using a solvent mixture acetonitrile/water/formic acid (79/20/1, v/v/v) and a dilution of raw extract using water/acetonitrile/formic acid (79/20/1, v/v/v) before instrumental analysis. Average recoveries in all three validated cereal crop types (maize, wheat, and barley), spiked at multiple levels, were found acceptable for all analytes when matrix-matched calibration was used, ranging from 63.2% to 111.2% and also showing very good repeatability, with relative standard deviations below 20%. Matrix effect (SSE) evaluation revealed maize as the most complex of the three analyzed cereal matrices, with strong SSE (<50% and >150%) recorded for all 11 analyzed mycotoxins. An additional method verification was performed through successful participation in proficiency testing schemes, with the achieved z-scores generally in the acceptable range of −2 ≤ z ≤ 2. The obtained validation results demonstrated the suitability of the developed confirmatory multi-mycotoxin UHPLC-MS/MS method based on a dilute-and-shoot principle for the simultaneous determination of low concentrations of 11 EU-regulated mycotoxins in cereals, including aflatoxins B1, B2, G1 and G2, deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, and ochratoxin A.
The changes of thyroid hormone in euthyroid patients with diabetes

Background Thyroid dysfunction is suggested to be associated with diabetes, but it is not clear if the thyroid hormone levels are associated with diabetes in euthyroid adults. Objective To investigate the association between thyroid hormone levels and diabetes in euthyroid adults. Methods Among the euthyroid adults who underwent health examination in West China Hospital, Sichuan University in 2016, patients with diabetes were identified according to the medical history, fasting blood glucose and HbA1c. Age and sex matched controls were identified from the population. The patients with diabetes group was further divided into two subgroups: patients with newly diagnosed diabetes (NDD) and with previously diagnosed diabetes (PDD). Independent t-test and multivariate logistic regression models were used to investigate the difference on the levels of thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3) and the ratio of FT4/FT3 between groups. Results We included 32,557 participants, 2,271 with diabetes and 1,187 NDD. Compared to the patients without diabetes, the odds ratios (ORs) per one unit of TSH, FT4, FT4/FT3 ratio and FT3 in patients with diabetes were 0.88 [95% confidence interval (CI): 0.82-0.95], 1.11 (95% CI: 1.08-1.14), 2.05 (95%CI: 1.81-2.32) and 0.85 (95%CI: 0.78-0.93), respectively. Compared to NDD group, the ORs per one unit of TSH, FT4, FT4/FT3 ratio and FT3 of the PDD group were 0.81 (95%CI: 0.71-0.92), 1.08 (95%CI: 1.04-1.12), 1.76 (95%CI: 1.49-2.08) and 1.01 (95%CI: 0.92-1.12), respectively. Conclusion In euthyroid adults, diabetes and its duration were significantly associated with thyroid hormone turnover. Patients with a previously diagnosed diabetes may be more likely to present elevated FT4/FT3 ratio, indicating an adaptive change of thyroid gland.
The Dimensionality of the Multidimensional Fatigue Inventory (MFI-20) Derived From Healthy Adults and Patient Subpopulations: A Challenge for Clinicians

Introduction: Fatigue is associated with various diseases of different origins extending from immune disorders to cancer. The purpose of this study was to explore the psychometric properties/dimensionality of the Multidimensional Fatigue Inventory (MFI-20) questionnaire in samples of healthy adults and patient subgroups in Greece. Methods: This was a multicenter cross-sectional study that included 80 women with breast cancer, 108 patients with multiple sclerosis (MS), 100 people with thalassemia diagnosis, and 185 healthy adults, aged 18-45 years. All patients were adults. Patients were recruited from a breast surgery clinic, a neurological clinic, and a thalassemia unit, while healthy adults were recruited from the University of West Attica students and personnel. The MFI-20, the modified fatigue impact scale (MFIS), the fatigue severity scale (FSS), and the Hamilton anxiety-depression scale (HANDS) were used. Internal consistency, repeatability, test-retest reliability, construct, and convergent validity were investigated. Results: MFI-20 exhibited excellent reliability properties (internal consistency: Cronbach’s alpha MFI-20 subscales ranged from 0.83 to 0.94; repeatability: Pearson’s r = 0.335 [p < 0.001]). Significant correlations were found between MFI-20 and MFIS: Pearson’s r = 0.870, FSS: Pearson’s r = 0.582 - 0.335, and HANDS: Pearson’s r = 0.734 - 0.442 (all p-values < 0.0001) on all subsamples. However, its dimensionality is questionable depending on the subpopulation tested, and the one-dimension perspective is possible. MS patients exhibited the highest total score (55.26 ± 16.53), while thalassemia patients exhibited the lowest score (45.09 ± 13.54). In all subscales, thalassemia patients differed statistically significantly from the MS patients (p < 0.01), while in the reduced activity subscale, thalassemia patients differed significantly from all other groups (p < 0.01). Conclusions: As strict fatigue subscale classification is questionable, the use of MFI-20 total score is suggested for the assessment of fatigue in clinical populations. As MFI-20 is a very useful research tool for studying fatigue, the use of the total and/or partial scores depends on the clinical population. Total score instead of (or additionally) partial scores is suggested in clinical practice.