Miracle-dz/UniFAR-FTUs huggingface dataset:
A research-based approach to generic graduate attributes policy

For many years universities around the world have sought to articulate the nature of the education they offer to their students through a description of the generic qualities and skills their graduates possess. Despite the lengthy history of the rhetoric of such policy claims, universities' endeavours to describe generic attributes of graduates continue to lack a clear theoretical or conceptual base and are characterized by a plurality of view-points. Furthermore, despite extensive funding in some quarters, overall, efforts to foster the development of generic attributes appear to have met with limited success. Recent research has shed some light on this apparent variability in policy and practice. It is apparent that Australian university teachers charged with responsibility for developing students' generic graduate attributes do not share a common understanding of either the nature of these outcomes, or the teaching and learning processes that might facilitate the development of these outcomes. Instead academics hold qualitatively different conceptions of the phenomenon of graduate attributes. This paper considers how the qualitatively different conceptions of graduate attributes identified in this research have been applied to the challenge of revising a university's policy statement specifying the generic attributes of its graduates. The paper outlines the key findings of the research and then describes how the university's revision of its policy statement has built upon this research, adopting a research-led approach to academic development. The resultant two-tiered policy is presented and the key academic development processes associated with the disciplinary contextualization of this framework are considered. The discussion explores some of the implications of this novel approach to structuring a university's policy, in particular, the variation in the relationship between discipline knowledge and generic attributes which was a key feature of the qualitative variation in understandings identified in the research.
Determinants of Malaysian Trade Balance: An ARDL Bound Testing Approach – A Commentary

Abstract This commentary is served as an additional light both from theoretical and empirical perspectives, on the study by Duasa (Global Economic Review, 2007, 36, pp. 89–102) who examined the short- and long-run relationships between trade balance, real exchange rates, income, and money supply for Malaysia. The final words I would like to make are that the results documented by Duasa require further investigation before it can be generalized.
The effect of mild aerobic physical activity on serum lipid, lipoprotein, and apolipoprotein concentrations in sedentary middle aged males

The purpose of this study was to determine if mild aerobic physical activity effected specific serum lipid concentrations to a greater extent than other serum lipid concentrations. Forty sedentary, healthy adult males (mean age 43.4 years) were divided into an untrained and trained groups. The trained members underwent twelve weeks of mild aerobic physical activity to determine the effects of mild aerobic physical activity on serum cholesterol, triglyceride, lipoprotein, and apolipoprotein concentrat ions . The trained subjects showed a significant increase in aerobic capacity (VO2 max) from 33.2 ml.kg. min-i to 39.1 ml.kg. min-i, while the untrained subjects remained stable at around 32.0 ml.kg. min-i . The serum cholesterol and lipoprotein concentrations showed no overall training effect. Both the untrained and untrained groups experienced a significant decrease in serum cholesterol (0.8 mmol /L & 1.1 mmol/L respectively) and low density lipoprotein (0.67 mmol/L & 0.84 mmol/L respectively) concentrations. Surprisingly, both groups showed a non significant decrease in high density lipoproteins of 0.19 mmol/L and 0.12 mmol/L respect ive ly . Meanwhile, the trained group showed a significant decrease in serum triglyceride (1.8 to 1.2 mmol/L) and apolipoprotein B (0.94 to 0.78 g/L). Although the untrained group also showed a significant decrease in apolipoprotein B (0.95 g/L to 0.88 g/L), the ratio of apolipoprotein A-1 to apolipoprotein B shown in the trained group was over double that of their untrained colleagues . This was due to the trained group maintaining a constant apolipoprotein A-1 level, which decreased in the untrained group, and the greater decrease observed in apolipoprotein B concentrations in the trained individuals. From these data it appears that a twelve week mild aerobic physical activity program effects the serum triglyceride and apolipoprotein concentrations with greater consistency and effectiveness, than either serum cholesterol or lipoprotein concentrat ions.
Hox genes and the evolution of adaptive phenotypes

Populations are faced with selective pressures that act on certain traits resulting in phenotypic divergence. The evolution of adaptive morphological traits is often associated with changes in pre-Existing structures. In semiaquatic insects, a dramatic growth of thoracic appendages is associated with their adaptation and efficient locomotion on the water surface. This particular leg allometry facilitated the exploitation of aquatic habitats, a restricted niche for their terrestrial relatives; and hence opens a new array of ecological opportunities. Additionally, the derived group of water striders has undergone further appendage modification, such that T2-Legs are longer than T3-Legs, a ground plan associated with the specialization to open water. Water striders have evolved a derived mode of locomotion through rowing on water. They move their mid-Legs in simultaneous sweeping strokes for propulsion, and move their hind-Legs in fine movements for orientation. Leg specification and elongation in semiaquatic insects happens during early embryogenesis as the newly hatching nymphs emerge with functional legs. The Hox transcription factor Ubx was found to be implicated in the reversal in leg ground plan. Nonetheless, the genetic mechanisms underlying these leg adaptive changes are still poorly understood. The presented thesis investigates these questions through two main goals: first, to uncover the genes and pathways implicated in the development and dramatic elongation of the legs; second, to examine the dynamics of Hox control responsible for the reversal in leg ground plan characteristic of water striders
Handbook of Avian Hybrids of the World

With more than 5,000 works cited, Handbook of Avian Hybrids of the World is the greatest compendium of information ever published on hybridization in birds. Worldwide in scope, it provides information on all reported avian crosses, not only those occurring in captivity, but also in a natural setting (approximately 4,000 crosses are covered). This book is a basic reference, intended both for the serious birder and the professional biologist. McCarthy's work fills a need for reference material that takes into account the last half century of data. It will be of interest to workers in a wide variety of fields, ranging from animal behavior to genetics, ecology, zoology, and systematics. In fact, it will make fascinating reading for anyone interested in birds and the natural world.
Studies on the production and application of monoclonal antibodies for the detection and treatment of cancer

Three aspects of the production and applications of monoclonal antibodies (mabs) were investigated. Two new cell lines (C23/11 and HET-37) were produced for use as potential fusion partners in human mab production. C23/11 was established from a blood sample obtained from a patient with myeloma. It was shown to have many of the cellular and antigenic properties associated with a lymphoblastoid cell line. HET-37 is a human/murine heteromyeloma cell line which was established after C23/11 cells were fused with murine NSO cells. Both C23/11 and HET-37 were characterised using electronmicroscopy, histology, immunophenotyping, isoenzyme analysis and DNA fingerprinting. In the second experimental section, three mab (HMFG-1, AUA-1 and H17E2) were labelled with the radioactive isotope, Indium-III. They were used for localisation of tumour in 21 patients with suspected ovarian carcinoma. IDIn-mab immunoscintigraphy was found to be a more sensitive technique for evaluation ovarian cancer than X-ray, CAT scan and ultrasound. Inln-mab immunoscintigraphy was particularly useful in localising tumours in patients with serum CA-125 levels greater than 100 IU/ml. F(ab’)2 fragments of HMFG-1 were labelled with technetium and were used to determine the distribution of ovarian tumour in a patient with minimal residual disease. In the final experimental section, HMFG-1 was chemically conjugated to the photosensitiser hematoporphyrin, Hp. HMFG-l-Hp was shown to kill antigen-bearing MCF-7 cells in vitro after irradiation. The HMFG-l-Hp conjugate had no effect on EJ- 138 cells under identical conditions. The EJ-138 cell line does not express the antigen to which the HMFG-1 antibody binds.
Predictors and moderators of treatment outcome in a randomized clinical trial for binge-eating disorder.

OBJECTIVE The current study examined predictors and moderators of two interventions for binge-eating disorder (BED). METHOD Participants were 112 adults with BED (Mage = 39.7 ± 13.4 years; MBMI = 35.1 ± 13.4 kg/m²; 82% female; 91% Caucasian) randomly assigned to integrative cognitive-affective therapy for BED (ICAT-BED) or guided self-help cognitive-behavioral therapy (CBTgsh). Generalized linear models examined predictors and moderators of objective binge-eating episode (OBE) frequency and OBE abstinence at end-of-treatment (EOT) and 6-month follow-up (FU). RESULTS Lower levels of baseline dietary restraint and emotion regulation difficulties predicted greater reductions in OBE frequency at EOT and FU, respectively. At EOT, greater pretreatment self-control predicted greater reductions in OBE frequency in ICAT-BED than CBTgsh (ps < .05). In addition, low shape/weight overvaluation predicted greater reductions in OBE frequency in ICAT-BED than CBTgsh, whereas high shape/weight overvaluation predicted comparable reductions in OBE frequency across treatments at EOT (ps < .02). At EOT and FU, greater baseline actual-ideal self-discrepancy predicted significantly greater reductions in OBE frequency in ICAT-BED, than CBTgsh (ps < .02). No significant predictor or moderator effects were observed for models examining OBE abstinence. CONCLUSION This study identified two general predictors and four moderators of BED treatment response. However, only one predictor (actual-ideal self-discrepancy) interacted with treatment type to differentially predict OBE frequencies at both EOT and FU. Altogether, findings suggest that ICAT-BED may confer specific and durable improvements in OBE frequencies among individuals with high actual-ideal self-discrepancy. Therefore, patients demonstrating these characteristics may be more likely to benefit from ICAT-BED. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
A Longitudinal Investigation of Falls and Motor Vehicle Crashes in Older Drivers

Objective: To assess the longitudinal association between fall history reported at a driver’s license screening visit and the likelihood of subsequent vehicle crashes. Method: A total of 1,127 older adults were recruited from Maryland State Motor Vehicle Administration sites and interviewed annually over 15 years. Results: Individuals who reported a previous fall were more likely to be female, perform worse on physical functioning and divided attention tasks, and report more situational driving avoidance compared with non-fallers at baseline. Females who reported a fall at baseline had a 2.6× greater likelihood of subsequently reporting a crash over the 15 years than males. Among those who reported a fall at baseline, greater weekly driving exposure over the 15 years was associated with a 23% higher likelihood of a subsequent crash. Discussion: These findings support the utility of investigating nontraditional driver screening methods to identify drivers who may be at increased risk of future driving difficulties.
Stigmatising attitudes towards people who inject drugs, and people living with blood borne viruses or sexually transmissible infections in a representative sample of the Australian population

Stigma has significant detrimental health outcomes for those affected. This study examined socio-demographic characteristics that were associated with stigmatising attitudes among the general population towards people who inject drugs, and people living with blood borne viruses or sexually transmissible infections. Questions were included in the Australian Survey of Social Attitudes (total sample = 1,001). Attitudes towards each of the target populations were measured by 5-item stigma scales. Bivariate analyses and multiple regression analyses were conducted to identify socio-demographic characteristics associated with stigmatising attitudes. Knowing a person affected by a stigmatised attribute was associated with reduced stigmatising attitudes, while voting for a conservative political party was associated with increased stigmatising attitudes. Age, gender, education, income, and marital status were each related to some stigmatising attitudes. Results also highlight differences between attitudes towards a stigmatised behaviour (i.e., injecting drug use) and stigmatised conditions (i.e., blood borne viruses and sexually transmissible infections). Identifying socio-demographic characteristics that are associated with stigmatising attitudes may have global implications for informing stigma reduction interventions, in order to promote positive health outcomes for affected communities.
Putting the microbiota to work: Epigenetic effects of early life antibiotic treatment are associated with immune-related pathways and reduced epithelial necrosis following Salmonella Typhimurium challenge in vitro

Salmonella enterica serovar Typhimurium is an animal welfare and public health concern due to its ability to parasite livestock and potentially contaminate pork products. To reduce Salmonella shedding and the risk of pork contamination, antibiotic therapy is used and can contribute to antimicrobial resistance. Here we hypothesized that immune system education by the microbiota can play a role in intestinal resilience to infection. We used amoxicillin (15mg/Kg) to modulate the intestinal microbiome of 10 piglets, paired with same age pigs that received a placebo (n = 10) from 0 to 14 days of age. Animals were euthanized at 4-weeks old. Each pig donated colon sections for ex vivo culture (n = 20 explants/pig). Explants were inoculated with S. Typhimurium, PBS or LPS (n = 6 explants/pig/group, plus technical controls). The gut bacteriome was characterized by sequencing of the 16S rRNA at 7, 21 days of age, and upon in vitro culture. Explants response to infection was profiled through high-throughput mRNA sequencing. In vivo antibiotic treatment led to β-diversity differences between groups at all times (P<0.05), while α-diversity did not differ between amoxicillin and placebo groups on day 21 and at euthanasia (P<0.03 on day 7). Explant microbiomes were not different from in vivo. In vitro challenge with S. Typhimurium led to lower necrosis scores in explants from amoxicillin-treated pigs, when compared to explants placebo-treated pigs (P<0.05). This was coupled with the activation of immune-related pathways in explants from amoxicillin-treated pigs (IL-2 production, NO production, BCR activation), when compared to placebo-treated pigs. In addition, several DNA repair and intestinal wound healing pathways were also only activated in explants from amoxicillin-treated pigs. Taken together, these findings suggest that immune education by the amoxicillin-disturbed microbiota may have contributed to mitigate intestinal lesions following pathogen exposure.
Molecular Diagnostic of Solid Tumor Using a Next Generation Sequencing Custom-Designed Multi-Gene Panel

Next generation sequencing (NGS) allows parallel sequencing of multiple genes at a very high depth of coverage. The need to analyze a variety of targets for diagnostic/prognostic/predictive purposes requires multi-gene characterization. Multi-gene panels are becoming standard approaches for the molecular analysis of solid lesions. We report a custom-designed 128 multi-gene panel engineered to cover the relevant targets in 22 oncogene/oncosuppressor genes for the analysis of the solid tumors most frequently subjected to routine genotyping. A total of 1695 solid tumors were analyzed for panel validation. The analytical sensitivity is 5%. Analytical validation: (i) Accuracy: sequencing results obtained using the multi-gene panel are concordant using two different NGS platforms and single-gene approach sequencing (100% of 83 cases); (ii) Precision: consistent results are obtained in the samples analyzed twice with the same platform (100% of 20 cases). Clinical validation: the frequency of mutations identified in different tumor types is consistent with the published literature. This custom-designed multi-gene panel allows to analyze with high sensitivity and throughput 22 oncogenes/oncosuppressor genes involved in diagnostic/prognostic/predictive characterization of central nervous system tumors, non-small-cell lung carcinomas, colorectal carcinomas, thyroid nodules, pancreatic lesions, melanoma, oral squamous carcinomas and gastrointestinal stromal tumors.
Epidemiological characteristics and incubation period of 7,015 confirmed cases with Coronavirus Disease 2019 outside Hubei Province in China

Abstract Background Disease caused by SARS-CoV-2 broke out in Wuhan in December 2019. We utilized confirmed cases outside Hubei Province to analyze epidemiologic characteristics and evaluate the effect of traffic restrictions implemented in Hubei beginning on January 23, 2020. Method Information on 7,015 confirmed cases from January 19 to February 8, 2020, in all provinces outside Hubei was collected from the national and local health commissions in China. Incubation period and interval times were calculated using dates of the following events: contact with an infected person, onset, first visit and diagnosis. We evaluated changes in incubation period and interval times. Results The average age of all cases was 44.24 years old. The median incubation period was 5 days and extended from 2 days on January 23 to 15 days on February 8. The proportion of imported cases decreased from 85.71% to 33.19% after January 23. In addition, the lengths of the intervals between onset and diagnosis, onset and first visit, and first visit and diagnosis decreased over time. Conclusion Rapidly transmitting COVID-19 has a short incubation period. The onset mainly occurs among young to middle-aged adults. Traffic restrictions played an important role in the decreased number of imported cases outside Hubei.
Advanced Quantification Methods To Improve the 18b Dormancy Model for Assessing the Activity of Tuberculosis Drugs In Vitro

One of the reasons for the lengthy tuberculosis (TB) treatment is the difficulty to treat the nonmultiplying mycobacterial subpopulation. In order to assess the ability of (new) TB drugs to target this subpopulation, we need to incorporate dormancy models in our preclinical drug development pipeline. In most available dormancy models, it takes a long time to create a dormant state, and it is difficult to identify and quantify this nonmultiplying condition. The Mycobacterium tuberculosis 18b strain might overcome some of these problems, because it is dependent on streptomycin for growth and becomes nonmultiplying after 10 days of streptomycin starvation but still can be cultured on streptomycin-supplemented culture plates. ABSTRACT One of the reasons for the lengthy tuberculosis (TB) treatment is the difficulty to treat the nonmultiplying mycobacterial subpopulation. In order to assess the ability of (new) TB drugs to target this subpopulation, we need to incorporate dormancy models in our preclinical drug development pipeline. In most available dormancy models, it takes a long time to create a dormant state, and it is difficult to identify and quantify this nonmultiplying condition. The Mycobacterium tuberculosis 18b strain might overcome some of these problems, because it is dependent on streptomycin for growth and becomes nonmultiplying after 10 days of streptomycin starvation but still can be cultured on streptomycin-supplemented culture plates. We developed our 18b dormancy time-kill kinetics model to assess the difference in the activity of isoniazid, rifampin, moxifloxacin, and bedaquiline against log-phase growth compared to the nonmultiplying M. tuberculosis subpopulation by CFU counting, including a novel area under the curve (AUC)-based approach as well as time-to-positivity (TTP) measurements. We observed that isoniazid and moxifloxacin were relatively more potent against replicating bacteria, while rifampin and high-dose bedaquiline were equally effective against both subpopulations. Moreover, the TTP data suggest that including a liquid culture-based method could be of additional value, as it identifies a specific mycobacterial subpopulation that is nonculturable on solid media. In conclusion, the results of our study underline that the time-kill kinetics 18b dormancy model in its current form is a useful tool to assess TB drug potency and thus has its place in the TB drug development pipeline.
Lymphocyte-Activation Gene 3 (LAG3) Protein as a Possible Therapeutic Target for Parkinson’s Disease: Molecular Mechanisms Connecting Neuroinflammation to α-Synuclein Spreading Pathology

Parkinson’s disease (PD) is the most common neurodegenerative movement disorder without any objective biomarker available to date. Increasing evidence highlights the critical role of neuroinflammation, including T cell responses, and spreading of aggregated α-synuclein in PD progression. Lymphocyte-activation gene 3 (LAG3) belongs to the immunoglobulin (Ig) superfamily expressed by peripheral immune cells, microglia and neurons and plays a key role in T cell regulation. The role of LAG3 has been extensively investigated in several human cancers, whereas until recently, the role of LAG3 in the central nervous system (CNS) has been largely unknown. Accumulating evidence highlights the potential role of LAG3 in PD pathogenesis, mainly by binding to α-synuclein fibrils and affecting its endocytosis and intercellular transmission, which sheds more light on the connection between immune dysregulation and α-synuclein spreading pathology. Serum and cerebrospinal fluid (CSF) soluble LAG3 (sLAG3) levels have been demonstrated to be potentially associated with PD development and clinical phenotype, suggesting that sLAG3 could represent an emerging PD biomarker. Specific single nucleotide polymorphisms (SNPs) of the LAG3 gene have been also related to PD occurrence especially in the female population, enlightening the pathophysiological background of gender-related PD clinical differences. Given also the ongoing clinical trials investigating various LAG3-targeting strategies in human diseases, new opportunities are being developed for PD treatment research. In this review, we discuss recent preclinical and clinical evidence on the role of LAG3 in PD pathogenesis and biomarker potential, aiming to elucidate its underlying molecular mechanisms.
Current concepts on haemorrhage control in severe trauma

The authors thank Susan Adams, FRACS, Sydney Children’s Hospital, University of New South Wales; S. V. Soundappan, FRACS, CTCPER, Senior Lecturer, Sydney Medical School; Zsolt J. Balogh, MD, PhD, FRACS, FACS, John Hunter Hospital and Hunter New England Health; Keith Simmons, MD, KND Consulting Pty Ltd; Professor Raphael Grzebieta BE (Hons1), MEngSci, PhD (Monash), FIEAust, CPEng, (NPER), APEC Engineer, IntPE(Aus), MSAEA, MSAE, RRSP; Adjunct Associate Professor George Rechnitzer (TARS-UNSW, VIFM-Monash University).