Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptome Profiling Reveals Enhanced Mitochondrial Activity as a Cold Adaptive Strategy to Hypothermia in Zebrafish Muscle

The utilisation of synthetic torpor for interplanetary travel once seemed farfetched. However, mounting evidence points to torpor-induced protective benefits from the main hazards of space travel, namely, exposure to radiation and microgravity. To determine the radio-protective effects of an induced torpor-like state we exploited the ectothermic nature of the Danio rerio (zebrafish) in reducing their body temperatures to replicate the hypothermic states seen during natural torpor. We also administered melatonin as a sedative to reduce physical activity. Zebrafish were then exposed to low-dose radiation (0.3 Gy) to simulate radiation exposure on long-term space missions. Transcriptomic analysis found that radiation exposure led to an upregulation of inflammatory and immune signatures and a differentiation and regeneration phenotype driven by STAT3 and MYOD1 transcription factors. In addition, DNA repair processes were downregulated in the muscle two days’ post-irradiation. The effects of hypothermia led to an increase in mitochondrial translation including genes involved in oxidative phosphorylation and a downregulation of extracellular matrix and developmental genes. Upon radiation exposure, increases in endoplasmic reticulum stress genes were observed in a torpor+radiation group with downregulation of immune-related and ECM genes. Exposing hypothermic zebrafish to radiation also resulted in a downregulation of ECM and developmental genes however, immune/inflammatory related pathways were downregulated in contrast to that observed in the radiation only group. A cross-species comparison was performed with the muscle of hibernating Ursus arctos horribilis (brown bear) to define shared mechanisms of cold tolerance. Shared responses show an upregulation of protein translation and metabolism of amino acids, as well as a hypoxia response with the shared downregulation of glycolysis, ECM, and developmental genes.
Volleyball Fundamentals

Learning to play volleyball has never been more effective or more fun than with Volleyball Fundamentals, Second Edition. Parents, coaches, and instructors of volleyball skill courses will immediately benefit from the solid foundational information and practical tips on how to teach every aspect of the sport. Players will enjoy using this resource because they will learn by doing spending less time reading and more time engaged in playing the game. Volleyball Fundamentals begins by teaching the basics that get players ready to take the court. The book covers positioning and the importance of reading the opposing players, communicating with teammates, and preparing for movement on the court. The guidance on formations and rotations will help students get an immediate sense of where they belong on the court. Clear instructions, accompanied by illustrations, for drills help players master specific skills such as serving, receiving a serve, setting, attacking, blocking, and digging. Each skill is introduced through a simple four-step sequence: • You Can Do It: The skill is introduced with sequential instructions and accompanying photographs. • More to Choose and Use: Variations and extensions of the primary skill are covered. • Take It to the Court: Readers learn how to apply the skill in game play. • Give It a Go: These sections provide direct experiences such as drills, small-sided games, and six-versus-six gamelike activities for the reader to use to gauge, develop, and hone the skill. Throughout the book, teaching cues offer advice for avoiding common mistakes. New to this edition is a chapter on how to effectively use the libero position (defensive specialist) in game play. Sections on offensive and defensive tactics, scoring systems, rules, and modified games have also been updated to make the game as easy for you to teach as it is for your students to learn. As part of the Sports Fundamentals Series, Volleyball Fundamentals will help you teach the basic skills and tactics of the game so that your players can compete on the court in no time at all.
Electromagnetic scattering by a bianisotropic cylinder

The problem of electromagnetic scattering by a homogeneous general uniaxial bianisotropic cylinder of arbitrary cross section is investigated via the generalized multipole technique (GMT) where only the longitudinal fictitious electric and magnetic filament currents are involved. Computed results of the internal field and bistatic echo width are presented, in which the results for a circular cylinder are in good agreement with the exact solution.
Broiler White Striping: A Review of Its Etiology, Effects on Production, and Mitigation Efforts

With an increase in the demand and production of chicken products, broilers have been bred to grow larger at a faster rate. This has led to several myopathies, one of which being white striping, which is now common to almost all broilers and is characterized as the deposition of fat within the broiler breast muscles. The purposes of this review are to examine the change in chicken production over the years, and to consider normal muscle growth physiology and the suspected pathological process and causes of white striping, as well as white striping visualization and detection in fast-growing broilers. With the increase in growth rate and size, multiple biochemical processes are affected in broilers, leading to hypoxia and inflammation, subsequent formation of foam cells from macrophages, and the deposition of fat in the form of white stripes along the muscle tissues. The negative effects of white striping include increased fat content and calories and decreased protein content, as well as reductions in palatability and drip loss. Thus, this review also explores the effects of white striping on meat quality, and finally evaluates several strategies attempted to mitigate white striping.
Spirometric Lung Functions in Type 2 Diabetes Mellitus: A Hospital-Based Study

Objective This cross-sectional case-control study was conducted with the aim to analyze spirometric lung functions in type 2 diabetes mellitus (T2DM) patients and to correlate the spirometric dysfunction with (a) duration of diabetes, b) metabolic control of diabetes, and c) microvascular complications of diabetes. Methods Pulmonary function tests (PFTs) were performed in 50 T2DM patients and 50 normal healthy controls aged <80 years by using an electronic spirometer. The PFTs recorded were as follows: forced vital capacity (FVC), forced expiratory volume in one second (FEV1), FEV1%, forced expiratory flow 25 (FEF25), forced expiratory flow 25-75 (FEF25-75), and peak expiratory flow rate (PEFR). The glycated hemoglobin (HbA1c) of all the patients was measured by affinity chromatography using the NycoCard HbA1C kit. The assessment of diabetic microvascular complications was performed as follows: peripheral neuropathy was done using Michigan Neuropathy Screening Instrument (MNSI), diabetic retinopathy using fundus examination, and diabetic nephropathy using solid phase/sandwich-format/immunometric assay using NycoCard U-albumin kit. PFTs of diabetic patients and controls were compared by applying an independent sample t-test. The correlation between FVC and FEV1, and HbA1c and duration of illness in diabetic patients was analyzed by applying the Pearson coefficient. Results The cases had low FVC (103.82 ±24.43 vs. 116.08 ±13.66), FEV1 (101.36 ±24.23 vs. 110.26 ±14.39), FEV1% (97.56 ±8.64 vs. 103.84 ±5.06), PEFR (101.52 ±27.18 vs. 116.96 ±14.96), and FEF 25-75 (73.56 ±29.19 vs. 98.40 ±14.45) compared to controls, and the difference was statistically significant. A significant negative correlation was found between spirometry parameters and duration of illness as well as HbA1c. Spirometric lung dysfunction also negatively correlated with microvascular complications of diabetes. Among various microvascular complications, retinopathy correlated best with various spirometric parameters. Conclusion Based on our findings, T2DM patients had a significant decrease in their spirometric indices. The pattern of spirometric dysfunction was suggestive of "mixed ventilatory dysfunction". The study results highlight the need to include PFTs in the periodic check-up as part of the comprehensive management of diabetic patients. Hence, pulmonary function should be included in the periodic comprehensive diabetic check for the holistic management of these patients.
Evaluation of Using Biochar and Compost on Soil and Olive Tree Water Status

ABSTRACT Improving soil fertility by adopting several sustainable management practices that are a prime concern for plant growth. Organic waste addition to agricultural soil is becoming a common practice that improves physical, chemical and hydrodynamic properties and minimizes environmental impact. The use of some alternative nutrition sources as biochar and compost has been tested on two-year-old olive varieties ‘Chemlali’ and ‘Koroneiki. ’ Three treatments were established “Cont” soil didn’t amended, “Comp” soil amended with Compost (90% soil+10% compost), and “Bioc” soil amended with biochar (90% soil+10% biochar). All treatments were conducted under deficit irrigation receiving 50% of daily evapotranspiration. We evaluated soil–plant water status and relationships between all studied parameters. Results showed that soil water content was the lowest in non-amended treatments and did not exceed 10%. During experiment, compost amendment induced a better water plant status for both studied varieties less than −2 MPa for mean seasonal leaf water potential, while the biochar amendment induced a weak stress level indicated by the lower potential that reached a minimum value equal to −2.9 MPa. Crop Water Stress Index on biochar-amended soil was more important than that measured on other treatments which confirms the sensitivity of biochar treatment to hard conditions. High negative relationships have been found between soil water statuses and plant water statuses. The highest determination coefficient was between crop water stresss index and leaf water potential was about 0.85. In conclusion, the use of organic amendment is an approach for sustainable olive orchards under low water avaibility.
Targeting Sphingosine 1-Phosphate Metabolism as a Therapeutic Avenue for Prostate Cancer

Simple Summary Prostate cancer (PC) is the most common cancer in men over 65 and the fifth leading cause of death by cancer in men. Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid that regulates cell proliferation, survival, differentiation, cell cycle, and apoptosis. S1P content and S1P-transforming enzymes activities are deregulated in several diseases including obesity, diabetes, neurological and cardiovascular disorders, and especially cancer. The implication of S1P metabolism in PC has been explored over the last 20 years in several in vitro and in vivo models of PC. Here we summarized (i) the most important findings about the role of S1P metabolism and its members in the oncogenesis of PC; and (ii) the most efficient molecules targeting S1P metabolism under preclinical and clinical development for curing PC. Abstract Prostate cancer (PC) is the second most common cancer in men worldwide. More than 65% of men diagnosed with PC are above 65. Patients with localized PC show high long-term survival, however with the disease progression into a metastatic form, it becomes incurable, even after strong radio- and/or chemotherapy. Sphingosine 1-phosphate (S1P) is a bioactive lipid that participates in all the steps of oncogenesis including tumor cell proliferation, survival, migration, invasion, and metastatic spread. The S1P-producing enzymes sphingosine kinases 1 and 2 (SK1 and SK2), and the S1P degrading enzyme S1P lyase (SPL), have been shown to be highly implicated in the onset, development, and therapy resistance of PC during the last 20 years. In this review, the most important studies demonstrating the role of S1P and S1P metabolic partners in PC are discussed. The different in vitro, ex vivo, and in vivo models of PC that were used to demonstrate the implication of S1P metabolism are especially highlighted. Furthermore, the most efficient molecules targeting S1P metabolism that are under preclinical and clinical development for curing PC are summarized. Finally, the possibility of targeting S1P metabolism alone or combined with other therapies in the foreseeable future as an alternative option for PC patients is discussed. Research Strategy: PubMed from INSB was used for article research. First, key words “prostate & sphingosine” were used and 144 articles were found. We also realized other combinations of key words as “prostate cancer bone metastasis” and “prostate cancer treatment”. We used the most recent reviews to illustrate prostate cancer topic and sphingolipid metabolism overview topic.
Regulation of Satiety by Bdnf-e2-Expressing Neurons through TrkB Activation in Ventromedial Hypothalamus

The transcripts for Bdnf (brain-derived neurotrophic factor), driven by different promoters, are expressed in different brain regions to control different body functions. Specific promoter(s) that regulates energy balance remain unclear. We show that disruption of Bdnf promoters I and II but not IV and VI in mice (Bdnf-e1−/−, Bdnf-e2−/−) results in obesity. Whereas Bdnf-e1−/− exhibited impaired thermogenesis, Bdnf-e2−/− showed hyperphagia and reduced satiety before the onset of obesity. The Bdnf-e2 transcripts were primarily expressed in ventromedial hypothalamus (VMH), a nucleus known to regulate satiety. Re-expressing Bdnf-e2 transcript in VMH or chemogenetic activation of VMH neurons rescued the hyperphagia and obesity of Bdnf-e2−/− mice. Deletion of BDNF receptor TrkB in VMH neurons in wildtype mice resulted in hyperphagia and obesity, and infusion of TrkB agonistic antibody into VMH of Bdnf-e2−/− mice alleviated these phenotypes. Thus, Bdnf-e2-transcripts in VMH neurons play a key role in regulating energy intake and satiety through TrkB pathway.
Clinical Symptoms Influencing Parkinson’s Patients’ Quality of Life in Latvia: A Single-Center Cohort Study

Background and Objectives: Parkinson’s disease (PD) is a chronic, progressive illness with a profound impact on health-related quality of life, and it is crucial to know what factors influence the quality of life throughout the course of the disease. This study aimed to evaluate PD patients’ motor and non-motor symptoms to compare symptom severity between PD clinical phenotypes and to assess the impact of disease symptoms on quality of life in a cohort of Latvian patients. Materials and Methods: We evaluated 43 patients with Parkinson’s disease. Fourteen patients had tremor dominant (TD) PD, twenty-five patients had postural instability/gait difficulty (PIGD), and four patients had a mixed phenotype. Results: The patients’ mean age was 65.21 years, and the disease’s mean duration was 7 years. The most common non-motor symptoms were fatigue (95.3%), sleep disturbance (83.7%), daytime sleepiness (83.7%), and pain and other sensations (81.4%). PIGD patients had a higher prevalence of depressed mood, daytime sleepiness, constipation, lightheadedness on standing, cognitive impairment, and severe gastrointestinal and urinary disturbances (as assessed using the SCOPA-AUT domains) compared with TD patients. A high prevalence of fatigue was assessed in both disease subtypes. Health-related quality of life significantly statistically correlated with MDS-UPDRS parts III and IV (r = 0.704), the Hoehn and Yahr scale (r = 0.723), as well as the SCOPA-AUT scale’s gastrointestinal (r = 0.639), cardiovascular (r = 0.586), thermoregulatory (r = 0.566) and pupillomotor domains (r = 0.597). Conclusions: The severity of motor symptoms, as well as non-motor symptoms, such as fatigue, apathy, sleep problems and daytime sleepiness, pain, and disturbances in gastrointestinal and cardiovascular function, negatively affect PD patients’ health-related quality of life. Thermoregulatory and pupillomotor symptoms also significantly affect PD patients’ well-being.
Metatranscriptomic Insights Into the Response of River Biofilm Communities to Ionic and Nano-Zinc Oxide Exposures

Manufactured Zn oxide nanoparticle (ZnO-NP) are extensively used world-wide in personal care and industrial products and are important contaminants of aquatic environments. To understand the overall impact of ZnO-NP contamination on aquatic ecosystems, investigation of their toxicity on aquatic biofilms is of particular consequence, given biofilms are known sinks for NP contaminants. In order to assess alterations in the functional activity of river microbial biofilm communities as a result of environmentally-relevant ZnO-NP exposure, biofilms were exposed to ionic zinc salt or ZnOPs that were uncoated (hydrophilic), coated with silane (hydrophobic) or stearic acid (lipophilic), at a total concentration of 188 μg l–1 Zn. ICP-MS analyses of biofilms indicated ZnO-NP concentrated in the biofilms, with hydrophilic, hydrophobic, and lipophilic treatments reaching 0.310, 0.250, and 0.220 μg Zn cm–2 of biofilm, respectively, while scanning transmission X-ray microspectroscopy (STXM) analyses of biofilms confirmed that Zn was extensively- and differentially-sorbed to biofilm material. Microbial community composition, based on taxonomic affiliation of mRNA sequences and enumeration of protozoa and micrometazoa, was not affected by these treatments, and the total transcriptional response of biofilms to all experimental exposures was not indicative of a global toxic-response, as cellular processes involved in general cell maintenance and housekeeping were abundantly transcribed. Transcripts related to major biological processes, including photosynthesis, energy metabolism, nitrogen metabolism, lipid metabolism, membrane transport, antibiotic resistance and xenobiotic degradation, were differentially expressed in Zn-exposures relative to controls. Notably, transcripts involved in nitrogen fixation and photosynthesis were decreased in abundance in response to Zn-exposure, while transcripts related to lipid degradation and motility-chemotaxis were increased, suggesting a potential role of Zn in biofilm dissolution. ZnO-NP and ionic Zn exposures elicited generally overlapping transcriptional responses, however hydrophilic and hydrophobic ZnO-NPs induced a more distinct effect than that of lipophilic ZnO-NPs, which had an effect similar to that of low ionic Zn exposure. While the physical coating of ZnO-NP may not induce specific toxicity observable at a community level, alteration of ecologically important processes of photosynthesis and nitrogen cycling are an important potential consequence of exposure to ionic Zn and Zn oxides.
Social entrepreneurship of small and medium-sized entrepreneurs in Thailand: influence of institutional environment, entrepreneurial passions, and entrepreneurial self-efficacy

Purpose This study aims to examine the relationship between the institutional environment, entrepreneurial passion and entrepreneurial self-efficacy, which affect small- and medium-sized entrepreneurs’ social entrepreneurial intentions. Design/methodology/approach A self-administered survey was used to collect data from 600 owner-managers of tourism and hospitality businesses on the Khon Kaen-Nong Khai railway route. Structural equation modeling was used to test the hypotheses using a two-step modeling approach. Findings Normative and cognitive environments positively influence entrepreneurial passion. However, regulatory environment was not significantly related to entrepreneurial passion. Entrepreneurial passion relies on entrepreneurial self-efficacy, a major mechanism that creates social entrepreneurial intention. Interestingly, entrepreneurial self-efficacy fully mediated the relationship between entrepreneurial passion and intention. Originality/value This study provides an original contribution to social entrepreneurship in terms of the role of self-efficacy in mediating the relationship between entrepreneurial passion and intention. The findings enhance theoretical viewpoints and aid in the practical implementation of the roles of small- and medium-sized entrepreneurs in social enterprises, supporting the entrepreneurial sector.
Brand Name Love: The Effects of Islamic Bracketed Brand Name on Perceived Quality and Purchase Intentions Retail Food Industry of Pakistan

This paper designs a 2x2-experiment research model within the subjects to analyze the effects of the “Islamic” bracketed brand name vs ordinary brand on consumer’s behavioral outcome-perceived quality and purchase intentions in the food industry of Pakistan. Islamic branding is a growing phenomenon and a large number of retailers in Islamic countries are using the word “Islamic” in brand design strategies. Two-way ANOVA for factorial analysis of variance is used with help of SPSS. The research findings reveal that Islamic origin brand names have a positive and significant impact on the perceived quality of products and consumer purchase intentions. Findings have important implications for retailers and SME businesses in Islamic countries in the brand-building process.
Phytochemical Profile, Antioxidant, Antimicrobial, and Antidiabetic Activities of Ajuga iva (L.)

In Morocco, many applications in ethnomedicine on Ajuga iva (L.) have been recognized as able to treat various pathologies such as diabetes, stress, and microbial infections. The objective of this work is to carry out phytochemical, biological, and pharmacological investigations on the extracts of Ajuga iva leaves in order to confirm its therapeutic effects. The phytochemical screening carried out on the different extracts of Ajuga iva showed its richness in primary (lipids and proteins) and secondary metabolites (flavonoids, tannins, reducing compounds, oses, and holoside. The best contents of polyphenols, flavonoids, and tannins evaluated by spectrophotometric methods were found in the hydroethanolic extract (69.850 ± 2.783 mg EAG/g DE, 17.127 ± 0.474 mg EQ/g DE, 5.566 ± 0.000 mg EQC/g DE), respectively. Analysis of the chemical composition of the aqueous extract by LC/UV/MS revealed 32 polyphenolic compounds including ferulic acid (19.06%), quercetin (10.19%), coumaric acid (9.63%), and apigenin-7-(2-O-apiosylglucoside) (6.8%). The antioxidant activity of Ajuga iva extracts was evaluated by three methods (DPPH*, FRAP, CAT). The hydroethanolic extract recorded the strongest reducing power: DPPH* (IC50 = 59.92 ± 0.7 µg/mL), FRAP (EC50 = 196.85 ± 1.54 (µg/mL), and CAT (199.21 ± 0.37 mg EAG/gE). A strong correlation between phenolic compounds and antioxidant activities was confirmed by the determination of Pearson’s coefficient. The antimicrobial activity of Ajuga iva studied by the microtiter method revealed potent antifungal and antibacterial qualities against Candida parapsilosis and Staphylococcus aureus BLACT. An in vivo oral glucose tolerance test (OGTT) using normal rats revealed that the antihyperglycemic action of the aqueous extract significantly reduced postprandial hyperglycaemia at (30 min, p < 0.01) and area under the curve (AUC glucose), p < 0.01. Similarly, the aqueous extract, tested on pancreatic α-amylase enzyme activity in vitro and in vivo significantly inhibited pancreatic α-amylase activity with IC50 = 1.52 ± 0.03 mg/mL. In conclusion, the extract from Ajuga iva could be a good source of bioactive molecules, which exhibit potent antioxidant and antimicrobial activity, as well as strong antidiabetic activity, for applications in the pharmaceutical industry.