Miracle-dz/UniFAR-FTUs huggingface dataset:
Quantitative Evaluation of the Transcriptional Activity of Steroid Hormone Receptor Mutants and Variants Using a Single Vector With Two Reporters and a Receptor Expression Cassette

Although the rapid development of high-throughput sequencing has led to the identification of a large number of truncated or mutated steroid hormone receptor (SHR) variants, their clinical relevance remains to be defined. A platform for functional analysis of these SHR variants in cells would be instrumental for better assessing their impact on normal physiology and SHR-associated diseases. Here we have developed a new reporter system that allows rapid and accurate assessment of the transcriptional activity of SHR variants in cells. The reporter is a single construct containing a firefly luciferase reporter gene, whose expression is under the control of a promoter with multiple steroid hormone responsive elements, and a Renilla luciferase reporter gene, that is constitutively expressed under the control of an internal ribosome entry site (IRES) and is not regulated by steroid hormones. The corresponding SHR (wildtype or mutant/variant) is also expressed from the same construct. Using this improved reporter system, we revealed a large spectrum of transactivation activities within a set of previously identified mutations and variations of the androgen receptor (AR), the estrogen receptor α (ERα) and the glucocorticoid receptor (GR). This novel reporter system enables functional analysis of SHR mutants and variants in physiological and pathological settings, offering valuable preclinical, or diagnostic information for the understanding and treatment of associated diseases.
The Navigation of Non–English Speaking Elderly Hispanic Immigrants through the Service System

This qualitative study examined three areas of social service provision through the experiences of non-English speaking older Hispanic immigrants. These three areas included availability and accessibility of services and culturally competent practices of service providers. The qualitative approach of phenomenology was used to interview ten participants in order to explore their experiences with service provision. An analysis of the interviews pointed to the following themes: (a) experiences with services were deeply connected to issues of immigration and adaptation; (b) language barriers were especially difficult to overcome in services; (c) the role of families as primary supports needed to be reconsidered in order to offer better quality of services; and (d) culturally competent practices needed to be reinforced at the organizational level of workers, agencies and government in order to become more effective. From these themes, recommendations and implications for social work and services for non-English speaking elderly Hispanics are detailed. III Acknowledgements Yolanda Luza Aliaga (my friend, companion and precious grandmother), Claribel Aliaga (my inspiration of will and courage), Jenny Trujillo (love of my life); and the wonderful friends in my life: Katia Lazaro, Wendy Louis, Bee Sack, Nilda Godhino.
Traumatic Events and PTSD Among Palestinian Children and Adolescents: The Effect of Demographic and Socioeconomic Factors

Background The situation in the Gaza Strip is uncommon in the frequency with which children are exposed to war-related traumatic events on a daily basis and because of the long-term nature of the conflict. The prevalence of posttraumatic stress disorder (PTSD) among children and adolescents in the Gaza Strip increased after the recent wars. The aims of the study are: To investigate the prevalence and nature of war traumatic events and PTSD; and to investigate how these traumatic events predict PTSD when taking into account demographic and socioeconomic status factors amongst Palestinian children and adolescents in the Gaza Strip. Methods The sample consists of 1,029 school pupils (11–17 years old): 533 (51.8%) were female and 496 (48.2%) were male. War-Traumatic Events Checklist (W-TECh) Post-Traumatic Stress Disorders Symptoms Scale (PTSDSS) were used. Results The majority of children and adolescents experienced personal trauma (N: 909; 88.4%), witnessed trauma to others (N: 861; 83.7%) and observed demolition of property (N: 908; 88.3%) during the war. Compared to girls, boys showed significantly more exposure to all three event types as well as overall traumatic events. Results also demonstrated that the prevalence of DSM-V PTSD diagnosis was 53.5% (N = 549). Further, children who had experienced personal trauma, trauma to others, and the demolition of property were significantly more likely to be diagnosed with PTSD compared to those who had not, even when adjusting for demographic and socioeconomic factors. The strongest war trauma for PTSD is personal trauma followed by witnessing trauma and then observing demolition of properties. Conclusions The study provides valuable evidence that demographic and socioeconomic factors mediate the relationship between different war traumatic events and PTSD. Interventions should take into account the children’s background including their gender, age, where they live, and their socioeconomic status (e.g., family income, parents' educational level, family size) to alleviate the psychological symptoms and to enhance their resilience.
Bistability of the Filchner‐Ronne Ice Shelf Cavity Circulation and Basal Melt

Circulation and water mass transformation within the Filchner-Ronne Ice Shelf (FRIS) cavity create precursors to Antarctic bottom water, which closes the global overturning circulation. This water mass transformation is contingent upon a relative low rate of FRIS basal melt, currently around 100–200 Gt/yr. Previous studies have indicated that Antarctic climate changes may induce intrusions of warm modified Warm Deep Water (mWDW) and an orderof-magnitude increase in basal melt, and signatures of mWDW have recently been observed along the face of the FRIS. However, it remains unclear how changes in near-Antarctic climate translate mechanistically to changes in mWDW access to the FRIS cavity. In this study a regional model is developed to investigate FRIS circulation dependence on local atmospheric state. Experiments with modified initial cavity conditions but identical atmospheric states yield bi-stable “warm” and “cold” FRIS cavity states, with an order-of-magnitude difference in basal melt rates. Idealized atmospheric perturbation experiments reveal that relatively modest perturbations to the katabatic winds shift the FRIS cavity between “warm” and “cold” states, which occur when the FRIS cavity is filled by mWDW or High Salinity Shelf Water (HSSW), respectively. The authors present a conceptual model in which the FRIS cavity state is determined by whether mWDW or HSSW is denser, and thus floods the cavity; these states are bi-stable because the basal melt rate feeds back on the salinity of HSSW. These findings highlight a key role for the katabatic winds in mediating the melt of the FRIS and other Antarctic ice shelves.
Basal Insulin Glargine (HOE 901) versus NPH Insulin in Patients With Type 1 Diabetes on Multiple Daily Insulin Regimes

WSTĘP. Insulina glargine (HOE 901, 21A-Gly-30Ba-L-Arg-30Bb-L-Arg typu ludzkiego) jest nowym rekombinowanym analogiem insuliny o przesunietym punkcie izoelektrycznym. Przesuniecie to powoduje zwolnienie szybkości wchlaniania i wydluza czas dzialania, co przypomina prawidlowe wydzielanie insuliny. Niedawno otrzymala ona akceptacje Food and Drug Administration. Celem niniejszego badania byla ocena dwoch roznych postaci insuliny glargine pod wzgledem bezpieczenstwa i skuteczności leczenia chorych na cukrzyce typu 1. MATERIAŁ I METODY. W ciągu 4 tygodni 256 chorych na cukrzyce typu 1 otrzymywalo insuline NPH lub insuline glargine zawierającą 30mg/ml cynku (insulina glargine [30]) lub 80 mg/ml cynku (insulina glargine [80]). Insulina glargine byla podawana raz na dobe — wieczorem przed snem. Insulina NPH byla podawana raz lub 2 razy dziennie (przed śniadaniem i przed snem) w zalezności od wcześniej stosowanego schematu leczenia. Dawka początkowa insuliny glargine oraz NPH byla uzalezniona od wcześniejszej lącznej dawki dobowej insuliny NPH. WYNIKI. W momencie zakonczenia badania w grupach otrzymujących insuline glargine stwierdzono znamiennie nizsze stezenie glukozy w osoczu na czczo (FPG, fasting plasma glucose) w porownaniu z grupą otrzymującą insuline NPH oraz zmniejszenie średniego skorygowanego stezenia FPG o 2,2 mmol/l (p = 0,0001). Insulina glargine skuteczniej niz insulina NPH obnizala stezenie FPG w grupie chorych wcześniej otrzymujących insuline NPH w 2 wstrzyknieciach dziennie, natomiast nie obserwowano roznic w grupie otrzymującej wcześniej pojedyncze wstrzykniecia insuliny NPH. Stezenie FPG bylo bardziej stabilne u chorych leczonych insuliną glargine niz u leczonych insuliną NPH. W wydzielonej podgrupie pacjentow (n = 71) w nocy wykonywano co godzine pomiary poziomu glukozy w osoczu. Insulina glargine powodowala obnizenia stezenie FPG po godzinie 5.00 rano; roznica osiągala znamiennośc statystyczną o godzinie 8.00 rano. Skorygowane stezenie FPG w czasie leczenia insuliną glargine [30] wynosily 7,8 mmol/l; insuliną glargine [80] — 7,3 mmol/l i insuliną NPH — 10,7 mmol/l. Oba preparaty insuliny glargine byly dobrze tolerowane, podobnie jak insulina NPH. WNIOSKI. Insulina glargine podawana raz na dobe przez 4 tygodnie jako metoda zapewnienia podstawowego stezenia insuliny u chorych na cukrzyce typu 1 leczonych wielokrotnymi wstrzyknieciami insuliny, bezpiecznie i skuteczniej niz insulina NPH obniza osoczowe stezenie glukozy na czczo.
Information technology and firm performance: mediation role of absorptive capacity and corporate entrepreneurship in manufacturing SMEs

ABSTRACT The success of small and medium-sized enterprises (SMEs) depends on dynamic capabilities, as it provides opportunities to remain competitive and perform well in the dynamic business environment. Although several studies show the significant positive impacts of dynamic capabilities on firm performance, our knowledge of the underlying mechanism through which IT capabilities affect the firm performance is not yet clear. Applying the dynamic capability view, this study investigates the intervening role of absorptive capacity and corporate entrepreneurship. The data of 417 respondents is collected from medium-sized manufacturing sector SMEs in Punjab, Pakistan. The research hypotheses have been analysed through the structural equation modelling method by using the Smart-PLS software. The research findings show that absorptive capacity and corporate entrepreneurship partially mediates the relationship between IT capabilities and firm performance. Furthermore, the sequential path of IT technical skills, absorptive capacity, corporate entrepreneurship, and firm performance is highly significant as compared to other paths.
Large Commercial Exposures and Tail Risk: Evidence from the Asia-Pacific P&C Insurance Market

We study losses in commercial insurance lines in the Asia-Pacific (APAC) region, by using a new dataset resulting from contributions by SCOR Services Asia-Pacific Pte Ltd and two large Lloyd’s syndicates, Hiscox and Liberty. We focus on man-made risks for commercial, manufacturing, and on-shore energy exposures, and provide a comparison of tail risk profiles within the APAC region, by allowing for important rating factors such as occupancy type and location. We find evidence of significant tail risk associated with large commercial risks (LCR), in particular for manufacturing exposures and developed APAC countries. The result is confirmed by the analysis of average loss severities for benchmark portfolios of LCR exposures. Developing APAC countries are characterized by lower tail risk than developed APAC countries; this wedge is shown to have increased over time as insurance penetration in the APAC region has increased.
IJSRD-International Journal for Scientific Research & Development| Vol. 3, Issue 10, 2015 | ISSN (online): 2321-0613

A suspension system or shock absorber is a mechanical device designed to smooth out or damp shock impulse, and dissipate kinetic energy. The shock absorbers duty is to absorb or dissipate energy and the helical spring is the most common element that has been used in suspension system. In this present study Non alloy steel helical spring will be used to suspend vehicle system. The uniform loading effect has been studied and Experimental, FEA analysis will be compared with analytical solution. Afterwards, the analysis will be done by considering different parameters. Experimental analysis will be done to validate the strength and modal analysis is done to determine the displacements for different loading condi-tion. Comparison is done by changing the wire diameter of the coil spring to check the best dimension for the spring in shock absorber. Modeling and Analysis is done by using Pro/ENGINEER and ANSYS respectively.
Men pause and women talk too much: Power and gender negotiations in eliciting data during semi-structured interviews

In most interpretive research, the relationship between the researcher and the participant is sometimes blurred, and the control over the research is often shared by both of them. Not only does power is manifested in all human interaction and all relations, but it is also changeable according to the situation in which they occur. In other words, power is not maintained by one person and resistance usually exists where power is found. However, researchers ought to be conscious of the power hierarchy that exists between the interviewer and the interviewee, which can be minimalized by practicing reflexivity which can minimize objectifying the interviewee and achieving self-awareness throughout the study. This interpretive research study reflects on the process of conducting the interview rather than simply analysing the interview data. I have adopted a phenomenological approach in this study in order to understand the participants’ own experiences and own description of the phenomena. The data revealed that the the power struggle during the interview with the two male interviewees was more prominent than with the female participant. The interview situation resulted in power tension instances between the interviewer and the participants.
The human capital effects of subsidized homeownership: Evidence from a natural experiment in Mumbai∗

Government programs in countries across all income levels subsidize homeownership and transfer wealth to households. Using subsidized housing lotteries in Mumbai, I identify the human capital effects of such a transfer and find that 3-5 years later, winners are more educated than non-winners, with effects concentrated among school-age youth. The intervention also increased rates of employment, particularly among older youth who have had the chance to complete their education. Effects are unlikely to be driven by relocation to areas with better opportunities, as winners live in neighborhoods with poorer school quality and lower rates of literacy and employment than non-winners. JEL Codes: E24, I38, O18, H24, J62 ∗This project has been supported by the J-PAL Governance Initiative, the Weiss Family Program Fund for Development Economics at Harvard University, the Institute of International Studies at the University of California, Berkeley, and the American Political Science Association Centennial Center. Research has been approved by the Committee for Protection of Human Subjects at the University of California, Berkeley, protocol 2017-04-9808. A pre-analysis plan has been registered with EGAP here (http://egap.org/registration/2810). Deviations from the pre-analysis plan are explained in Appendix A. This paper uses the same research design and data as Kumar 2019. I am extremely grateful for Partners for Urban Knowledge Action Research and particularly Nilesh Kudupkar for assistance with data collection. Thank you to Pradeep Chhibber, Joel Middleton, Edward Miguel, and Alison Post for their mentorship and advice throughout this project. Anustubh Agnihotri, Christopher Carter, Nirvikar Jassal, Curtis Morrill, Pranav Gupta, Carlos Schmidt-Padilla, Michael Koelle, Matthew Stenberg, and participants at DevPec 2019 also provided valuable comments. Most importantly, I thank the hundreds of survey and interview respondents who gave their time to this study. †Travers Department of Political Science. tkumar[at]berkeley[dot]edu
Efficacy of a Green Banana–Mixed Diet in the Management of Persistent Diarrhea: Protocol for an Open-Labeled, Randomized Controlled Trial

Background Diarrhea is the second-leading cause of death in children under 5 years of age. In low- and middle-income countries, 3%-20% of acute diarrheal episodes become persistent diarrhea (PD) (ie, duration ≥14 days), which results in 36%-56% of all diarrheal deaths. In Bangladesh, PD causes >25% of diarrhea-related deaths. Commensal gut microbiota dysbiosis is increasingly recognized in the pathogenesis of PD. Hospital-based management of PD requires a hospital stay, which increases the risk of infection and hospital costs. The higher cost of treatment and high case-fatality rates reiterate PD as an important public health problem. At the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), for the last two decades, a consensus-based guideline has been followed for PD. Observation has revealed that green banana helps in the resolution of diarrhea. However, no larger prospective study has been conducted to evaluate the efficacy of green banana in the management of PD among children older than 6 months of age. Objective Our objective is to assess the efficacy of full-strength rice suji (semolina) with and without green banana compared to three-quarter-strength rice suji in the management of PD in children aged 6-36 months at the Dhaka Hospital of the icddr,b. Methods This open-labeled, randomized controlled study aims to enroll a total of 145 children with PD who have not been improving on a diet of milk suji. Children will be randomized into three different diet-specific groups: full-strength rice suji containing green banana, full-strength rice suji alone, and three-quarter-strength rice suji. The primary outcome is the percentage of children who recovered from diarrhea by day 5. Results Recruitment and data collection began in December 2017 and were completed in November 2019. Results are expected by April 2020. Conclusions This study is expected to provide insights into the incorporation of green banana into the dietary management of PD. This would be the first study to investigate the role of microbiota and metabolomics in the pathogenesis of PD. Trial Registration ClinicalTrials.gov NCT03366740; https://clinicaltrials.gov/ct2/show/NCT03366740 International Registered Report Identifier (IRRID) DERR1-10.2196/15759
Identification and characterization of lactic acid bacteria isolated from cow milk and olives brine

A total of 24 strains of lactic acid bacteria were isolated from cow milk and olives brine, out of which 4 strains, having the best criteria of conservation, were selected for their identification and their characterization. These lactic isolates underwent preliminary testing of morphological and biochemical such as: gram reaction, catalase test, mobility and morphology. Other tests were held for characterization such as: fermentation type, fermentation mode of sugars, tolerance to heat, salinity and pH, test of Voges Proskauer, hydrolysis test of Citrate and Arginine. The lactic isolates were classified into 3 species as follows: the BLN3 and BLN4 strains belong to the species Lactobacillus plantarum, the BLN8 strain belongs to Lactobacillus delbrueckii, and the BLN17 strain is classified as Lactobacillus fermentum. BLN3 has only one particularity; she has high tolerance to grow in salt and acid medium.
Association of Genetic Polymorphism of Inflammatory Genes (IL-1β and IL-4) with Diabetes Type 2

It is known that genetic and environmental factors may influence susceptibility to type 2 diabetes (T2D). Pancreatic inflammation of T2D patients is associated with pathophysiology of diabetes type 2. In this study, we investigated role of polymorphism of IL-1β (rs16944) and IL4 gene and environmental factors in susceptibility to diabetes type 2. 190 diabetics and 210 healthy controls of central India were recruited. Anthropometric and lifestyle data were collected during sampling. Genetic polymorphism study of IL-1β gene was undertaken using PCR-RFLP meanwhile IL-4 polymorphism was investigated using PCR-VNTR (Variable number of tandem repeats) analysis. Genotype, allele frequencies and carriage rates of IL-1β and IL-4 polymorphisms were recorded. Diabetic patients had higher levels of fasting plasma glucose (P<0.0001), HbA1c (P<0.0001) and Post prandial glucose (P<0.0001) compared to control subjects. Nominal difference observed for LDL-C (P=0.0462), triglyceride (P=0.0024). Diabetic patients had higher levels of weight of women (P=0.0024), Men (P=0.0157) and BMI of Women (P=0.0388), WHR in Women (P<0.0001) and WHR in Men (P=0.0147). Smoking habit data suggest odds ratio of smokers were 1.205 but statistically difference is not significant. Overall distribution of IL-β-511 genotypes was significantly different in Control as compared to diabetic patients. The frequencies of TT genotype (p =0.0047) and T allele ((P=0.0001) were significantly higher in patients than controls and associated with higher risk. An odds ratio of 1.781 of ‘T’ allele showed stronger association of overall “T’ allele frequency in diabetes susceptibility. Overall IL-4 distribution of genotype was weakly but significantly different in case and control populations. Genotype distribution of IL-4 suggest association of B2B2 (p =0.0347) genotype with susceptibility to diabetes type 2 but allele frequency and carriage rate was not found significantly different. Our findings suggest association of IL-β-511 and weak association of IL-4 genotype as well as obesity with diabetes type 2.
TPM Vol

There exists a strong left-right divide in abortion attitudes, with the political right being more antiabortion. Popular rhetoric suggests that this divide is explained by the right’s amplified concern about the humanness of the preborn, but recent research questions this position, instead positing greater sexism on the right as a mechanism. We re-examined this latter assumption in the United States (N = 296) and consider an additional explanation: those on the right (vs. left) are especially likely to experience shared reality with key political figureheads, for instance, the then-nominated Supreme Court Justice Neil Gorsuch, which further facilitates their antiabortion stance. We find support for the conservatism ➔ sexism ➔ antiabortion pattern, but shared reality as mediator overrides the effect of sexism. With no sex differences observed, antiabortion positions appear to be more about individual differences in ideology and epistemic motivations than group membership or sexism. Implications and future directions are discussed.