Miracle-dz/UniFAR-FTUs huggingface dataset:
The Biotechnological Potential of the Marine Diatom Skeletonema dohrnii to the Elevated Temperature and pCO2

Marine diatoms are promising candidates for biotechnological applications, since they contain high-value compounds, naturally. To facilitate the production of these compounds, stress conditions are often preferable; however, challenges remain with respect to maximizing a metabolic potential for the large-scale cultivation. Here, we sequenced the transcriptome of diatom Skeletonema dohrnii under the actual (21 °C, 400 ppm) and elevated (25 °C, 1000 ppm) temperature and pCO2 condition. Results indicated that cells grown at higher temperature and pCO2 showed increasing growth rate, pigment composition, and biochemical productivity as did the expression of chlorophyll, carotenoid and bioactive compound related genes or transcripts. Furthermore, performing de novo transcriptome, we identified 32,884 transcript clusters and found 10,974 of them were differentially expressed between these two conditions. Analyzing the functions of differentially expressed transcripts, we found many of them involved in core metabolic and biosynthesis pathways, including chlorophyll metabolism, carotenoid, phenylpropanoid, phenylalanine and tyrosine, and flavonoid biosynthesis was upregulated. Moreover, we here demonstrated that utilizing a unique bio-fixation ability, S. dohrnii is capable of suppressing central carbon metabolism to promote lipid productivity, fatty acid contents and other bioactive compounds under high temperature and pCO2 treatment. Our study suggests that this S. dohrnii species could be a potential candidate for wide-scale biotechnological applications under elevated temperature and CO2 conditions.
New Donor-Acceptor Stenhouse Adducts as Visible and Near Infrared Light Polymerization Photoinitiators

Polymerization photoinitiators that can be activated under low light intensity and in the visible range are being pursued by both the academic and industrial communities. To efficiently harvest light and initiate a polymerization process, dyes with high molar extinction coefficients in the visible range are ideal candidates. In this field, Donor-acceptor Stenhouse Adducts (DASA) which belong to a class of recently discovered organic photochromic molecules still lack practical applications. In this work, a series of DASA-based dyes are proposed as photoinitiators for the free radical polymerization of (meth)acrylates upon exposure to a near infrared light (laser diode at 785 nm).
Ecological Studies and Trophic State Evaluation of a Tropical Impoundment in West Africa

Weekly base-line data (September 1983 … March 1984) on plankton community structure, abundance, and primary productivity of a small (A = 3.7 ha, Zm = 16m) water-supplying reservoir (Kongingiri Dam, alt. 1343 m) in Jos Plateau, Nigeria are presented. Supporting ecological observations include thermal and optical properties, pH, conductivity, dissolved oxygen, alkalinity, chloride and dissolved organic matter. Water characteristics of the exosystem showed varying turbidity (Secchi=1.0−2.8 m), a circumneutral character (pH, x=7.1), predominance of bicarbonate (xx=0.26 meq/l) system, and moderate ionic composition (xx=23 μS/cm). The plankton community was dominated by Bacillariophyceae and Chlorophyceae in population density, and Chlorophyceae in species richness. The important forms were Nitzschia spp. Synedra ulna, Navicula spp. Melosira italica, Pinnularia sp. Staurastrum spp. , Cosmarium, Closterium, Eurastrum platycerum, Micrasterias americana, M. crux-melitensis, Teilingia granulata Desmidium swartzii, Scenedesmus sp. Pediastrum boryanum. The zooplankton community had numerical dominance of rotifers. The principal zooplankton species included Brachionus falcatus, Keratella cochlearis, K. tropica, Trichocerca similis, Pompholyx complanatus, Bosmina longirostris, Ceriodaphnia cornuta, and species of Mesocyclops, Thermocyclops, and copepod nauplii. The ecological characteristics and productivity rates indicate an oligo-mesotrophic state of the aquatic ecosystem.
Social Integration and Diurnal Cortisol Decline: The Role of Psychosocial and Behavioral Pathways.

OBJECTIVE A growing number of studies have associated various measures of social integration, the diversity of social roles in which one participates, with alterations in hypothalamic-pituitary-adrenocortical (HPA) functioning. The pathways through which social integration may be linked to HPA functioning, however, are as yet unknown. The present study examined whether daily social interactions, affective responses, health behaviors, and personality help explain the association between social integration and diurnal cortisol slope. METHODS A sample of 456 healthy, employed adults (53.9% female, 82.0% White, 72.2% BA or greater, mean age of 42.86) completed a 4-day ecological momentary assessment protocol that measured cortisol, social interactions, affect, sleep, and physical activity at frequent intervals throughout the day. Social integration was measured at baseline. RESULTS Regression results controlling for age, sex, race, and education indicated that more socially integrated individuals showed steeper cortisol slopes (B = -0.00253, p = .006). Exploratory analyses suggested that the consistency (i.e. reduced variability) in nightly sleep midpoint partially explained this association (B = -0.00042, 95% CI: -0.00095, -0.00001). Personality, mood, social interaction patterns, and non-sleep health behavior differences did not account for the association between social integration and HPA activity. CONCLUSION This study replicates previous findings linking social integration and HPA functioning, and it examines patterns of nightly sleep as possible pathways through which the association may operate. Results have implications for understanding mechanisms for health risk and for development of future interventions.
Clinical risk factors and predictive tool of bacteremia in patients with cirrhosis

Objective We aimed to analyze the risk factors and to establish a predictive tool for the occurrence of bloodstream infections (BSI) in patients with cirrhosis. Methods A total of 2888 patients with cirrhosis were retrospectively included. Multivariate analysis for risk factors of BSI were tested using logistic regression. Multivariate logistic regression was validated using five-fold cross-validation. Results Variables that were independently associated with incidence of BSI were white blood cell count (odds ratio [OR] = 1.094, 95% confidence interval [CI] 1.063–1.127)], C-reactive protein (OR = 1.005, 95% CI 1.002–1.008), total bilirubin (OR = 1.003, 95% CI 1.002–1.004), and previous antimicrobial exposure (OR = 4.556, 95% CI 3.369–6.160); albumin (OR = 0.904, 95% CI 0.883–0.926), platelet count (OR = 0.996, 95% CI 0.994–0.998), and serum creatinine (OR = 0.989, 95% CI 0.985–0.994) were associated with lower odds of BSI. The area under receiver operating characteristic (ROC) curve of the risk assessment scale was 0.850, and its sensitivity and specificity were 0.762 and 0.801, respectively. There was no significant difference between the ROC curves of cross-validation and risk assessment. Conclusions We developed a predictive tool for BSI in patients with cirrhosis, which could help with early identification of such episodes at admission, to improve outcome in these patients.
Pathways and Mechanisms for Removal of Dissolved Organic Carbon from Leaf Leachate in Streams

Removal of dissolved organic carbon (DOC) from water resulting from adsorption and microbial uptake was examined to determine the importance of biotic and abiotic pathways. Physical–chemical adsorption to components of the stream sediment or water and biotic assimilation associated with the microbial population was determined in recirculating chambers utilizing leachate from alder (Alnus rubra). Adsorptive mechanisms were further separated into interactions involving (1) specific clay minerals, (2) amorphous solid phases of hydrous aluminum and iron oxides, and (3) fine particulate organic matter. Physical–chemical adsorptive mechanisms for alder leachate removal exhibited rapid kinetic equilibration between the DOC and solid phases, but only a specific fraction of the DOC, likely containing certain chemical functional groups, was adsorbed. The amorphous aluminum and iron oxides possessed a much higher potential capacity than the clay minerals or fine particulate organics for DOC adsorption. Microbial upt.
Simple Fabrication of Transparent, Colorless, and Self-Disinfecting Polyethylene Terephthalate Film via Cold Plasma Treatment

Cross-infection following cross-contamination is a serious social issue worldwide. Pathogens are normally spread by contact with germ-contaminated surfaces. Accordingly, antibacterial surface technologies are urgently needed and have consequently been actively developed in recent years. Among these technologies, biomimetic nanopatterned surfaces that physically kill adhering bacteria have attracted attraction as an effective technological solution to replace toxic chemical disinfectants (biocides). Herein, we introduce a transparent, colorless, and self-disinfecting polyethylene terephthalate (PET) film that mimics the surface structure of the Progomphus obscurus (sanddragon) wing physically killing the attached bacteria. The PET film was partially etched via a 4-min carbon tetrafluoride (CF4) plasma treatment. Compared to a flat bare PET film, the plasma-treated film surface exhibited a uniform array structure composed of nanopillars with a 30 nm diameter, 237 nm height, and 75 nm pitch. The plasma-treated PET film showed improvements in optical properties (transmittance and B*) and antibacterial effectiveness over the bare film; the transparency and colorlessness slightly increased, and the antibacterial activity increased from 53.8 to 100% for Staphylococcus aureus, and from 0 to 100% for Escherichia coli. These results demonstrated the feasibility of the CF4 plasma-treated PET film as a potential antibacterial overcoating with good optical properties.
CIB4 is essential for the haploid phase of spermatogenesis in mice†

Abstract Spermatogenesis is a complex developmental process that involves the proliferation of diploid cells, meiotic division, and haploid differentiation. Many genes are shown to be essential for male fertility using knockout (KO) mice; however, there still remain genes to be analyzed to elucidate their molecular mechanism and their roles in spermatogenesis. Calcium- and integrin-binding protein 1 (CIB1) is a ubiquitously expressed protein that possesses three paralogs: CIB2, CIB3, and CIB4. It is reported that Cib1 KO male mice are sterile due to impaired haploid differentiation. In this study, we discovered that Cib4 is expressed strongly in mouse and human testis and begins expression during the haploid phase of spermatogenesis in mice. To analyze the function of CIB4 in vivo, we generated Cib4 KO mice using the CRISPR/Cas9 system. Cib4 KO male mice are sterile due to impaired haploid differentiation, phenocopying Cib1 KO male mice. Spermatogenic cells isolated from seminiferous tubules demonstrate an essential function of CIB4 in the formation of the apical region of the sperm head. Further analysis of CIB4 function may shed light on the etiology of male infertility caused by spermatogenesis defects, and CIB4 could be a target for male contraceptives because of its dominant expression in the testis. Summary sentence Cib4 mutant mice exhibit male infertility due to impaired spermiogenesis.
Differential Glucocorticoid-Dependent Regulation and Function of the ERRFI1 Gene in Triple-Negative Breast Cancer

Abstract Glucocorticoids (GCs; eg, hydrocortisone [CORT]) are routinely used as chemotherapeutic, anti-emetic, and palliative agents in breast cancer (BCa) therapy. The effects of GC signaling on BCa progression, however, remain a contentious topic as GC treatment seems to be beneficial for receptor-positive subtypes but elicits unfavorable responses in triple-negative BCa (TNBC). The mechanistic basis for these conflicting effects of GC in BCa is poorly understood. In this study, we sought to decipher the molecular mechanisms that govern the GC-dependent induction of the tumor suppressor ERRFI1 gene, an inhibitor of epidermal growth factor receptor (EGFR) signaling, and characterize the role of the GC-ERRFI1 regulatory axis in TNBC. Treatment of TNBC cell lines with a protein synthesis inhibitor or GC receptor (GR) antagonist followed by gene expression analysis suggests that ERRFI1 is a direct GR target. Using in silico analysis coupled with enhancer-reporter assays, we identified a putative ERRFI1 enhancer that supports CORT-dependent transactivation. In orthogonal assays for cell proliferation, survival, migration, and apoptosis, CORT mostly facilitated an oncogenic phenotype regardless of malignancy status. Lentiviral knockdown and overexpression of ERRFI1 showed that the CORT-enhanced oncogenic phenotype is restricted by ERRFI1 in the normal breast epithelial model MCF10A and to a lesser degree in the metastatic TNBC line MDA-MB-468. Conversely, ERRFI1 conferred pro-tumorigenic effects in the highly metastatic TNBC model MDA-MB-231. Taken together, our findings suggest that the progressive loss of the GC-dependent regulation and anti-tumorigenic function of ERRFI1 influences BCa progression and may contribute to the unfavorable effects of GC therapy in TNBC.
The aroma of TEMED as an activation and stabilizing signal for the antibacterial enzyme HEWL

The unpleasant smell released from dead bodies, may serve as an alarm for avoiding certain behaviour or as feeding or oviposition attractants for animals. However, little is known about their effect on the structure and function of proteins. Previously, we reported that using the aroma form of TEMED (a diamine), representative of the "smell of death", could completely inhibit the fibril formation of HEWL, as an antibacterial enzyme, and a model protein for fibrillation studies. To take this further, in this study we investigated the kinetics of TEMED using a number of techniques and in particular X-ray crystallography to identify the binding site(s) of TEMED and search for hotspot(s) necessary to inhibit fibril formation of HEWL. Structural data, coupled with other experimental data reported in this study, revealed that TEMED completely inhibited fibril formation and stabilized the structure of HEWL through enhancement of the CH-Π interaction and binding to an inhibitor hotspot comprised of residues Lys33, Phe34, Glu35 and Asn37 of HEWL. Additionally, results from this study showed that the binding of TEMED increased the activity and thermal stability of HEWL, helping to improve the function of this antibacterial enzyme. In conclusion, the role of the "smell of death”, as an important signal molecule affecting the activity and stability of HEWL was greatly highlighted, suggesting that aroma producing small molecules can be signals for structural and functional changes in proteins.
3D Mesh Releasing Method: A Retrospective Analysis of Fractional CO2 Treatment on Contracture Scars

There has been reports on fractional CO2 laser successfully improving contracture scars that impair the function of a joint. It seems that certain contracture problems could be solved by laser instead of surgery. However, the clinical application could be difficult when the efficacy of the method remains unknown. The purpose of this study is to report the releasing capacity of the fractional CO2 laser on contracture scars based on a defined treatment method.
Population Study of Ovarian Cancer Risk Prediction for Targeted Screening and Prevention

Unselected population-based personalised ovarian cancer (OC) risk assessment combining genetic/epidemiology/hormonal data has not previously been undertaken. We aimed to perform a feasibility study of OC risk stratification of general population women using a personalised OC risk tool followed by risk management. Volunteers were recruited through London primary care networks. Inclusion criteria: women ≥18 years. Exclusion criteria: prior ovarian/tubal/peritoneal cancer, previous genetic testing for OC genes. Participants accessed an online/web-based decision aid along with optional telephone helpline use. Consenting individuals completed risk assessment and underwent genetic testing (BRCA1/BRCA2/RAD51C/RAD51D/BRIP1, OC susceptibility single-nucleotide polymorphisms). A validated OC risk prediction algorithm provided a personalised OC risk estimate using genetic/lifestyle/hormonal OC risk factors. Population genetic testing (PGT)/OC risk stratification uptake/acceptability, satisfaction, decision aid/telephone helpline use, psychological health and quality of life were assessed using validated/customised questionnaires over six months. Linear-mixed models/contrast tests analysed impact on study outcomes. Main outcomes: feasibility/acceptability, uptake, decision aid/telephone helpline use, satisfaction/regret, and impact on psychological health/quality of life. In total, 123 volunteers (mean age = 48.5 (SD = 15.4) years) used the decision aid, 105 (85%) consented. None fulfilled NHS genetic testing clinical criteria. OC risk stratification revealed 1/103 at ≥10% (high), 0/103 at ≥5%–<10% (intermediate), and 100/103 at <5% (low) lifetime OC risk. Decision aid satisfaction was 92.2%. The telephone helpline use rate was 13% and the questionnaire response rate at six months was 75%. Contrast tests indicated that overall depression (p = 0.30), anxiety (p = 0.10), quality-of-life (p = 0.99), and distress (p = 0.25) levels did not jointly change, while OC worry (p = 0.021) and general cancer risk perception (p = 0.015) decreased over six months. In total, 85.5–98.7% were satisfied with their decision. Findings suggest population-based personalised OC risk stratification is feasible and acceptable, has high satisfaction, reduces cancer worry/risk perception, and does not negatively impact psychological health/quality of life.
Local Government Competition and Regional Green Development in China: The Mediating Role of Environmental Regulation

Green development is an important way to meet the challenges of ecological and environmental protection and economic growth, as well as an inevitable choice to realize China’s sustainable development in the new era. The Chinese economic system is such that local government competition has become a key factor affecting regional green development under the current leadership. Based on the inter-provincial panel data of 30 provinces in mainland China from 1997 to 2017, this paper uses the total-factor non-radial directional distance function and slack-based measure data envelopment analysis (SBM-DEA) to measure the green development efficiency of the provinces. Additionally, it also uses the Malmquist–Luenberger (ML) index to decompose green development efficiency and analyzes its internal driving factors. Finally, taking environmental regulation as a mediating variable, this paper empirically analyzes the influence mechanism of local government competition on green development efficiency from three perspectives including growth competition, fiscal competition and investment competition. The study found that: the green development efficiency of Chinese regions showed a downward trend, with significant regional differences; technological progress is the key factor to improve the efficiency of green development, and its role gradually decreases from eastern to western and central regions; pure technical efficiency has become a bottleneck restricting the improvement of green development efficiency, while scale efficiency shows significant regional differences; the growth competition, fiscal competition and investment competition of local government all have a significant inhibitory effect on the efficiency of green development. This paper puts forward policy suggestions supporting enterprise technology research and development, optimizing energy conservation and emission reduction as well as improving the local government performance evaluation system for green development.
The Role of Lipid Metabolism in COVID-19 Virus Infection and as a Drug Target

The current Coronavirus disease 2019 or COVID-19 pandemic has infected over two million people and resulted in the death of over one hundred thousand people at the time of writing this review. The disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Even though multiple vaccines and treatments are under development so far, the disease is only slowing down under extreme social distancing measures that are difficult to maintain. SARS-COV-2 is an enveloped virus that is surrounded by a lipid bilayer. Lipids are fundamental cell components that play various biological roles ranging from being a structural building block to a signaling molecule as well as a central energy store. The role lipids play in viral infection involves the fusion of the viral membrane to the host cell, viral replication, and viral endocytosis and exocytosis. Since lipids play a crucial function in the viral life cycle, we asked whether drugs targeting lipid metabolism, such as statins, can be utilized against SARS-CoV-2 and other viruses. In this review, we discuss the role of lipid metabolism in viral infection as well as the possibility of targeting lipid metabolism to interfere with the viral life cycle.
Daily Coping With Social Identity Threat in Outgroup-Dominated Contexts: Self-Group Distancing Among Female Soldiers

We examine the degree to which women in a male-dominated field cope with daily experiences of social identity threat by distancing themselves from other women. A daily experience-sampling study among female soldiers (N = 345 data points nested in 61 participants) showed women to self-group distance more on days in which they experienced more identity threat. This was mediated by daily concerns about belonging but not achievement in the military, supporting the explanation that women distance from other women as a way to fit in a masculine domain. However, on a daily basis, self-group distancing did not appear to protect women’s outcomes as it was related to lower daily well-being and motivation. The findings indicate that targets are not passive recipients of identity threat but active agents coping daily with the challenges they face, but that regulation strategies may also incur costs. Implications for theories on coping with stigma and costs are discussed.