Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of oat hay and leguminous forage mixture feeding on enteric methane emission, energy utilization, and feed conversion efficiency in male crossbred Simmental beef cattle.

Dietary manipulation has the potential to mitigate methane (CH4 ) emission and to maintain or enhance livestock productivity. We conducted two experiments to investigate the effects of replacing oat hay by leguminous forages (alfalfa hay [AH], 0, 8, 16, and 24%, experiment 1; common vetch hay [CVH], 0, 10, 20, and 30%, experiment 2) on energy metabolism of crossbred Simmental cattle. In experiment 1, total volatile fatty acid (VFA) concentrations increased quadratically with increasing AH proportions (p = .006) with a forage-to-concentrate ratio of approximately 50:50, whereas the CH4 energy to gross energy intake ratio (CH4 -E:GEI) was significantly lower with 16% AH compared with 24% AH diet (p < .05). In experiment 2, there were no differences in the total VFA concentrations among the four diet groups with a forage-to-concentrate ratio of around 60:40 (p > .05); however, CH4 -E:GEI was significantly lower in the 30% CVH diet compared with the 10% CVH diet (p < .05). There was no significant difference in feed conversion efficiency among the four diet groups in each experiment. The results suggest that substituting 16 and 30% oat hay by AH and CVH provide optimal diets with forage-to-concentrate ratios of 50:50 and 60:40, respectively, which may reduce CH4 emission without compromising the feed conversion efficiency of crossbred Simmental cattle.
Dimensionless Index in Patients With Low-Gradient Severe Aortic Stenosis and Preserved Ejection Fraction

Supplemental Digital Content is available in the text. Background Risk stratification of patients with low-gradient (LG) severe aortic stenosis (AS) despite preserved left ventricular ejection fraction remains challenging. We sought to evaluate the relationship between the dimensionless index (DI)—the ratio of the left ventricular outflow tract time-velocity integral to that of the aortic valve jet—and mortality in these patients. Methods Seven hundred fifty-five patients with LG severe AS (defined by aortic valve area ≤1 cm2 or aortic valve area indexed to body surface area ≤0.6 cm2/m2 and mean aortic pressure gradient <40 mm Hg) and preserved left ventricular ejection fraction ≥50% were studied. Flow status was defined according to stroke volume index <35 mL/m2 (low flow, LF) or ≥35 mL/m2 (normal flow, NF). Results After adjustment for age, sex, body mass index, Charlson comorbidity index, history of hypertension, history of atrial fibrillation, AS-related symptoms, left ventricular ejection fraction, indexed left ventricular ventricular mass, aortic valve area, and aortic valve replacement as a time-dependent covariate, patients with LG-LF and DI<0.25 exhibited a considerable increased risk of death compared with patients with LG-NF and DI≥0.25 (adjusted hazard ratio, 2.41 [95% CI, 1.61–3.62]; P<0.001), LG-NF and DI<0.25 (adjusted hazard ratio, 1.84 [95% CI, 1.24–2.73]; P=0.003), and LG-LF and D≥0.25 (adjusted hazard ratio, 2.27 [95% CI, 1.42–3.63]; P<0.001). In contrast, patients with LG-LF and DI≥0.25, LG-NF and DI<0.25, and LG-NF and DI≥0.25 had similar outcome. DI<0.25 showed incremental prognostic value in patients with LG-LF severe AS but not in patients with LG-NF severe AS. Conclusions Among patients with LG severe AS and preserved left ventricular ejection fraction, decreased DI<0.25 is a reliable parameter in patients with LF to identify a subgroup of patients at higher risk of death who may derive benefit from aortic valve replacement.
Response to the Comment on "Prognosis-based Definition of Resectability in Pancreatic Cancer: A Road Map to New Guidelines".

Reply: We thank Professor Miao and co-authors for their comments on our manuscript. Correctly, the authors stated that the pathologic staging of pancreatic cancer has an impact on prognosis. We strongly agree with this statement, however, parameters such T, N M, and R status, are not accurately known before surgery. The aim of our study was to develop a prognostic instrument able to identify the patients who can benefit from a surgical resection. This instrument is to be used before surgery to understand the pros and cons of proceeding with resection. Many of the variables indicated by Professor Miao and co-authors drive prognosis, but cannot be accurately evaluated before surgery, therefore are not as useful in patient selection. We also agree that Tumor, Node, Metastasis (TNM) staging has changed over time, and through the various editions. However, both American Joint Committee on Cancer (AJCC) seventh and eighth editions define T4 in a similar way (arterial involvement). This is why we compared the outcome of T4 patients vs. the others. We also sought to point out that not all patients with T4 (but M0) disease should be ruled out for a surgical approach. We agree that the percentage of T4 patients included in the study was low and that there was definitely a selection bias. We do not believe that radiological ‘‘downstaging’’ should be the only sign of tumor response needed to select a patient for surgery. We agree that pathological T stage has a prognostic role in pancreatic cancer (the majority of data available are, however, from an era before very effective chemotherapy). One issue is that cancer. Br J Surg. 2019;106:1257.
The Effect of Baseline Performance and Age on Cognitive Training Improvements in Older Adults: A Qualitative Review

Findings that the brain is capable of plasticity up until old age have led to interest in the use of cognitive training as a potential intervention to delay the onset of dementia. However, individuals participating in training regimens differ greatly with respect to their outcomes, demonstrating the importance of considering individual differences, in particular age and baseline performance in a cognitive domain, when evaluating the effectiveness of cognitive training. In this review, we summarize existing literature on cognitive training in adults across the domains of episodic memory, working memory and the task-switching component of executive functioning to clarify the picture on the impact of age and baseline performance on cognitive training-related improvements. Studies targeting episodic memory induced greater improvements in younger adults with more intact cognitive abilities, explained in part by factors specific to episodic memory training. By contrast, older, lower baseline performance adults improved most in several studies targeting working memory in older individuals as well as in the majority of studies targeting executive functioning, suggesting the preservation of neural plasticity in these domains until very old age. Our findings can have important implications for informing the design of future interventions for enhancing cognitive functions in individuals at the prodromal stage of Alzheimer’s Disease and potentially delaying the clinical onset of Alzheimer’s Disease. Future research should more clearly stratify individuals according to their baseline cognitive abilities and assign specialized, skill-specific cognitive training regimens in order to directly answer the question of how individual differences impact training effectiveness.
Clinical Characteristics and Outcomes of Coronavirus Disease 2019 Patients Who Received Compassionate-Use Leronlimab

Abstract Background Leronlimab, a monoclonal antibody blocker of C-C chemokine receptor type 5 originally developed to treat human immunodeficiency virus infection, was administered as an open-label compassionate-use therapeutic for coronavirus disease 2019 (COVID-19). Methods Twenty-three hospitalized severe/critical COVID-19 patients received 700 mg leronlimab subcutaneously, repeated after 7 days in 17 of 23 patients still hospitalized. Eighteen of 23 received other experimental treatments, including convalescent plasma, hydroxychloroquine, steroids, and/or tocilizumab. Five of 23 received leronlimab after blinded, placebo-controlled trials of remdesivir, sarilumab, selinexor, or tocilizumab. Outcomes and results were extracted from medical records. Results Mean age was 69.5 ± 14.9 years; 20 had significant comorbidities. At baseline, 22 were receiving supplemental oxygen (3 high flow, 7 mechanical ventilation). Blood showed markedly elevated inflammatory markers (ferritin, D-dimer, C-reactive protein) and an elevated neutrophil-to-lymphocyte ratio. By day 30 after initial dosing, 17 were recovered, 2 were still hospitalized, and 4 had died. Of the 7 intubated at baseline, 4 were fully recovered off oxygen, 2 were still hospitalized, and 1 had died. Conclusions Leronlimab appeared safe and well tolerated. The high recovery rate suggested benefit, and those with lower inflammatory markers had better outcomes. Some, but not all, patients appeared to have dramatic clinical responses, indicating that unknown factors may determine responsiveness to leronlimab. Routine inflammatory and cell prognostic markers did not markedly change immediately after treatment, although interleukin-6 tended to fall. In some persons, C-reactive protein clearly dropped only after the second leronlimab dose, suggesting that a higher loading dose might be more effective. Future controlled trials will be informative.
CBCT image quality QA: Establishing a quantitative program

Abstract Purpose Routine quality assurance (QA) of cone‐beam computed tomography (CBCT) scans used for image‐guided radiotherapy is prescribed by the American Association of Physicists in Medicine Task Group (TG)‐142 report. For CBCT image quality, TG‐142 recommends using clinically established baseline values as QA tolerances. This work examined how image quality parameters vary both across machines of the same model and across different CBCT techniques. Additionally, this work investigated how image quality values are affected by imager recalibration and repeated exposures during routine QA. Methods Cone‐beam computed tomography scans of the Catphan 604 phantom were taken on four TrueBeam® and one Edge™ linear accelerator using four manufacturer‐provided techniques. TG‐142 image quality parameters were calculated for each CBCT scan using SunCHECK Machine™. The variability of each parameter with machine and technique was evaluated using a two‐way ANOVA test on a dataset consisting of 200 CBCT scans. The impact of imager calibration on image quality parameters was examined for a subset of three machines using an unpaired Student’s t‐test. The effect of artifacts appearing on CBCTs taken in rapid succession was characterized and an approach to reduce their appearance was evaluated. Additionally, a set of baselines and tolerances for all image quality metrics was presented. Results All imaging parameters except geometric distortion varied with technique (P < 0.05) and all imaging parameters except slice thickness varied with machine (P < 0.05). Imager calibration can change the expected value of all imaging parameters, though it does not consistently do so. While changes are statistically significant, they may not be clinically significant. Finally, rapid acquisition of CBCT scans can introduce image artifacts that degrade CBCT uniformity. Conclusions This work characterized the variability of acquired CBCT data across machines and CBCT techniques along with the impact of imager calibration and rapid CBCT acquisition on image quality.
Assessment of knowledge and perceptions towards diabetes mellitus and its associated factors among people in Debre Berhan town, northeast Ethiopia

Background Globally, diabetes is a major public health burden that results in more than 3.2 million adult deaths per year. Currently, diabetes is increasingly becoming a major threat to global public health, particularly in Sub-Saharan Africa. Although previous studies emphasized knowledge and health beliefs about diabetes among patients living with diabetes, there is minimal evidence about knowledge and perception of risk for developing diabetes at the community level. Objective This study aimed to assess the knowledge and perception of diabetes mellitus and its associated factors among people in Debre Berhan town, northeast Ethiopia. Methods A community-based cross-sectional study was conducted among 423 participants. The study was carried out from 25 February to 10 March 2019. Data were collected using a structured pretested questionnaire through face-to-face interviews. Data were entered into Epi data V 3.1 and exported to SPSS V 24 for analysis. A variable with p< 0.2 in bivariable analysis was entered into multivariable logistic regression. During multivariate analysis, variables with a p value of ≤ 0.05 were considered significantly associated. Result A total of 237 (56.02%) participants had good general knowledge about diabetes mellitus. In the multivariable analysis, participants who were single (AOR = 9.08, CI: 1.72–48), had a family history of diabetes (AOR = 2.83; CI: 1.10–7.24), and had exposure to health education (AOR = 3.27; CI: 2.02–5.31) were associated with good knowledge. In this study, few respondents (20.1%) felt that they had a higher risk of developing diabetes. Two-thirds of respondents (62.4%) saw diabetes is a serious disease. On the other hand, approximately 67% agreed to the perceived benefits of screening. Conclusion Almost half of the Debre Berhan community was found to have inadequate knowledge of diabetes mellitus. Married, higher educational status, exposure to health education, and family history of diabetes mellitus were significantly associated with good knowledge. The perceived risk of developing diabetes was low at the community level, although many respondents felt that behavior change is important in the prevention of diabetes. Therefore, policy makers, healthcare managers, and healthcare workers need to work cooperatively to foster community knowledge towards diabetes mellitus.
Influence of three different anesthesia protocols on aged rat brain: a resting-state functional magnetic resonance imaging study

Abstract Background Resting-state functional magnetic resonance imaging (rs-fMRI) is a promising method for the study of brain function. Typically, rs-fMRI is performed on anesthetized animals. Although different functional connectivity (FC) in various anesthetics on whole brain have been studied, few studies have focused on different FC in the aged brain. Here, we measured FC under three commonly used anesthesia methods and analyzed data to determine if the FC in whole brain analysis were similar among groups. Methods Twenty-four male aged Wistar rats were randomly divided into three groups (n = 8 in each group). Anesthesia was performed under either isoflurane (ISO), combined ISO + dexmedetomidine (DEX) or α-chloralose (AC) according to the groups. Data of rs-fMRI was analyzed by FC in a voxel-wise way. Differences in the FC maps between the groups were analyzed by one-way analysis of variance and post hoc two-sample t tests. Results Compared with ISO + DEX anesthesia, ISO anesthesia caused increased FC in posterior brain and decreased FC in the middle brain of the aged rat. AC anesthesia caused global suppression as no increase in FC was observed. Conclusion ISO could be used as a substitute for ISO + DEX in rat default mode network studies if the left temporal association cortex is not considered important.
Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Blood for the Diagnosis of Bloodstream Infections: A Mini-Review

Staphylococcus aureus represents a major human pathogen able to cause a number of infections, especially bloodstream infections (BSI). Clinical use of methicillin has led to the emergence of methicillin-resistant S. aureus (MRSA) and MRSA-BSI have been reported to be associated with high morbidity and mortality. Clinical diagnosis of BSI is based on the results from blood culture that, although considered the gold standard method, is time-consuming. For this reason, rapid diagnostic tests to identify the presence of methicillin-susceptible S. aureus (MSSA) and MRSA isolates directly in blood cultures are being used with increasing frequency to rapidly commence targeted antimicrobial therapy, also in the light of antimicrobial stewardship efforts. Here, we review and report the most common rapid non-molecular and molecular methods currently available to detect the presence of MRSA directly from blood.
Long-Term Effects of Neglect on Violence: Are They Spurious or Indirect?

Abstract Purpose In the present paper we explore the long-term influence of childhood neglect on violent behavior in the transition to adulthood. In particular, we test whether neglect is spuriously related to violence due to their common association with academic achievement, physical abuse, and general offending. We then ask whether neglect has an indirect effect on violence through its impact on parental attachment, alcohol use, emotional negativity, academic achievement, or staying in school. Methodology/approach We use two waves of data from the National Longitudinal Survey of Adolescent Health (Add Health) and employ both regression models and INDIRECT, a syntax file that allows for the testing of indirect effects using SPSS (Preacher & Hayes, 2008). Findings We find that the long-term association between childhood neglect and violence in the transition to adulthood is robust in models controlling for GPA, physical abuse, and other forms of offending. Neglect did not have an indirect effect on violence through attachment, negative emotionality, or academic achievement but did have indirect effects on violence through its association with staying in school and with alcohol use. Research implications This set of analyses was exploratory in nature. Further research on neglect should be undertaken, using finely tuned measures and research questions. In addition, our findings imply that the association between neglect and later violent behavior may be intertwined with certain dynamics of physical abuse and alcohol use, which should be further studied.
Direct evidence of Hymenoscyphus fraxineus infection pathway through the petiole‐shoot junction

Summary The symptoms of ash dieback caused by the fungus Hymenoscyphus fraxineus include wilting of the foliage followed by dieback of shoots, twigs and branches. Necroses in shoots are assumed to develop after infection through leaf petioles; however, clear evidence of this infection pathway has not yet been provided. Considering the multiple pathogen genotypes in dead ash petioles, we aimed to obtain a spatial overview of all H. fraxineus genotypes colonizing individual shoots and their corresponding petioles before leaf shedding to acquire precise information about the infection biology of H. fraxineus and its ability to cross the petiole-shoot junction. Individual genotypes of H. fraxineus were characterized by the analysis of microsatellites using DNA extracted directly from petiole segments or cultures isolated from the segments. We detected 150 different multilocus genotypes in 10 analysed shoots and their respective petioles; the highest number of genotypes was eight for a single petiole and three for a single shoot. The genotypes of most shoot lesions were identical to particular genotypes from the proximal segments of petioles, implicating the main pathway of shoot infections. To test whether the amount of colonized substrate or intraspecific competition have an effect on successful infection, genotypes that reached the most proximal end of the petioles were scored for the number of invaded petiole segments and for the number of other H. fraxineus genotypes co-occurring in the segments. However, the extent of colonization of the scored genotypes and intraspecific competition with other H. fraxineus strains did not influence pathogen success in entering the shoot. This study confirms that the majority of ash shoot infections are caused by genotypes of H. fraxineus originating from petioles. Compared to petioles, the frequency of shoot colonization as well as number of H. fraxineus genotypes in shoots was much lower.
Is the cognitive system much more robust than anticipated? Dual-task costs and residuals in working memory.

Several working memory (WM) theories assume a resource sharing between the maintenance of information and its processing, whereas other theories suppose that these 2 functions of WM rely on different pools of resources. Studies that addressed this question by examining whether dual-task costs occur in tasks combining processing and storage have led to mixed results. Whereas some of them reported symmetric dual-task costs, others found no or negligible effects, while still others found a reduction in performance in memory but not in processing. In the present experiment, we tested whether these discrepancies in results might be due to participants strategically prioritizing one component of the task over the other. Thus, we asked participants to perform at their maximum level (i.e. span level) in one component of the dual-task and assessed performance on the other. In line with resource-sharing views, results indicated that performing at span on 1 task strongly degraded performance on the other, with symmetric costs. However, important residuals in both processing and storage suggested an unexpected resilience of the cognitive system that any resource-sharing theory must take into account. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Azithromycin to Prevent Pertussis in Household Contacts, Catalonia and Navarre, Spain, 2012–2013

We retrospectively assessed the effectiveness of azithromycin in preventing transmission of pertussis to a patient’s household contacts. We also considered the duration between symptom onset in the primary patient and azithromycin administration. We categorized contacts into 4 groups: those treated within <7 days, 8–14 days, 15–21 days, and >21 days after illness onset in the primary patient. We studied 476 primary index patients and their 1,975 household contacts, of whom 4.5% were later identified as having pertussis. When contacts started chemoprophylaxis within <21 days after the primary patient’s symptom onset, the treatment was 43.9% effective. Chemoprophylaxis started >14 days after primary patient’s symptom onset was less effective. We recommend that contacts of persons with pertussis begin chemoprophylaxis within <14 days after primary patient’s symptom onset.
New Class of Efficient T2 Magnetic Resonance Imaging Contrast Agent: Carbon-Coated Paramagnetic Dysprosium Oxide Nanoparticles

Nanoparticles are considered potential candidates for a new class of magnetic resonance imaging (MRI) contrast agents. Negative MRI contrast agents require high magnetic moments. However, if nanoparticles can exclusively induce transverse water proton spin relaxation with negligible induction of longitudinal water proton spin relaxation, they may provide negative contrast MR images despite having low magnetic moments, thus acting as an efficient T2 MRI contrast agent. In this study, carbon-coated paramagnetic dysprosium oxide (DYO@C) nanoparticles (core = DYO = DyxOy; shell = carbon) were synthesized to explore their potential as an efficient T2 MRI contrast agent at 3.0 T MR field. Since the core DYO nanoparticles have an appreciable (but not high) magnetic moment that arises from fast 4f-electrons of Dy(III) (6H15/2), the DYO@C nanoparticles exhibited an appreciable transverse water proton spin relaxivity (r2) with a negligible longitudinal water proton spin relaxivity (r1). Consequently, they acted as a very efficient T2 MRI contrast agent, as proven from negative contrast enhancements seen in the in vivo T2 MR images.