Miracle-dz/UniFAR-FTUs huggingface dataset:
Characteristics of the posttraumatic stress disorder traumatic stressor : a study of rural and northern first responders

A diagnosis of posttraumatic stress disorder (PTSD) requires meeting Criterion A, which states that the individual must: 1) experience, witness, or be confronted with an event that involved actual or threatened death, serious injury, or threat to one’s physical integrity (Criterion A1); and 2) experience intense fear, helplessness, or horror during or shortly after the event (Criterion A2). Despite various attempts to define Criterion A, a strong etiological link between the event and resulting PTSD has remained elusive. The overarching purpose of the current study was to examine characteristics of traumatic stressors beyond Criterion A. A cross-sectional, repeated-measures design was used to examine Criterion A1 events under two conditions: when an event was associated with lasting distress (i.e., distressing event) and when an event was not associated with lasting distress (i.e., control event). This research addressed four objectives. First, it identified characteristics of Criterion A1 events that evoked extreme amounts of distress (i.e., PTSD symptoms). Second, it examined whether these characteristics were more relevant for distressing events compared to control events. Third, it tested whether event characteristics added incremental value in predicting PTSD symptoms above meeting Criterion A. Finally, it tested hypothesized relationships between event characteristics and processes implicated in cognitive models, namely peritraumatic dissociation and posttrauma cognitions. The present study surveyed 181 first responders from northern British Columbia. First responders repeatedly experience Criterion A1 events, which allowed them to rate the relevance of event characteristics for both types of events. A principal component analysis of diverse event characteristics revealed that distressing events were characterized by chaos and resource limitations, which were both rated as significantly more descriptive of distressing events compared to control events. As hypothesized, both event characteristics predicted PTSD
International multicenter randomized phase III study of first-line erlotinib (E) followed by second-line cisplatin plus gemcitabine (CG) versus first-line CG followed by second-line E in advanced non-small cell lung cancer (aNSCLC): The TORCH trial.

7508 Background: In a placebo-controlled trial, E prolonged survival of unselected aNSCLC patients (pts) not eligible for further chemotherapy. Two phase II studies suggested that first-line treatment with E might be a valid alternative to chemotherapy. Methods: An independent non profit phase 3 trial was designed to test whether overall survival in the experimental arm, first-line E (150 mg/d po) followed at progression by CG (C 80 mg/m2 d1 + G 1,200 mg/m2 dd1&8, q3w), was not inferior than in the standard first-line CG followed at progression by E. Stage IV/IIIb (with supraclavicular nodes or pleural effusion) NSCLC pts, PS 0-1, were eligible. With a 1.25 upper limit of 95% CI of hazard ratio (HR) of death, 80% power, 0.025 1-sided alpha, a sample size of 900 pts was calculated. Secondary end-points: progression-free survival, response, toxicity, quality of life, cost-efficacy analysis, correlative studies on tumor and blood samples. Results: From Dec 06 to Nov 09, 760 pts were randomized, 380 in each a.
Quantitative biogeography of Orthoptera does not support classical qualitative regionalization of the Carpathian Mountains

The first biogeographical division of the Carpathians, the second largest mountain range in Europe, was based on qualitative observational floristic data > 100 years ago and has also been applied for the regional zoogeography. In this study, the recent availability of detailed quantitative data allowed us to perform a more powerful evaluation of the classical biogeographical regions of the area. Thus, we analysed updated distribution patterns of 137 Orthoptera species native to the Carpathian Mountains and, by using published species range maps, we compiled data on species presence or absence within 2576 cells of a 10 km × 10 km universal transverse mercator grid in the area. Pattern analysis of the data was based on non-metric multidimensional scaling and clustering using six different algorithms applied to a β sim dissimilarity matrix. The unweighted pair-group method using arithmetic averages, which gave the best performance in the analysis of species turnover, delineated four regions. Environmental variables and species richness were used in logistic regression as predictors of delineated clusters, and indicator species were identified for each of the inferred regions. The pattern can be explained, in part, by environmental variables and species richness (34.2%) and was also influenced by connections with the orthopterofauna from adjacent areas. The observed discrepancy between regionalization based on expert knowledge and the pattern revealed using quantitative data provides a warning that the biogeography of the Carpathians might also have been revised in other taxa, where only classical qualitative regionalization exists.
The complete mitochondrial genome of red-throated flycatcher Ficedula albicilla (Passeriformes: Ficedula)

Abstract We report the mitochondrial genome of Ficedula albicilla. The overall base composition of F. albicilla mitogenome is 29.49%A, 15.06%G, 32.98%C, and 22.47%T, with an A + T content of 51.96%. The total length of the sequence is 16,791 bp (13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control regions). Phylogenetic analysis was performed based on the concatenated nucleotide sequences of cytochrome c oxidase subunit I and cytochrome b using the neighbor-joining method and the Kimura 2-parameter model in MEGA 7.0 with 1000 bootstrap replicates.
Study of road traffic accidental deaths (RTA) in and around Bastar Region of Chhattisgarh

To analyze the magnitude of head injury in fatal RTA cases, present study was conducted in the department of Forensic Medicine Toxicology, Govt. Medical College, Jagdalpur (Chhattisgarh) in 2009 calendar year. The present study was undertaken on 105 victims of RTA who died due to head injuries, which autopsied at GMC, Jagdalpur (C.G). Most of the accidents occurred in the afternoon hours (12: 01 - 18:00). There was a clear male dominance (88.57%). The most affected age was middle age (21-40 yrs) & most commonly affected age group is 21-30 yrs. Vehicular occupants were commonly affected (63.80%) & amongst them two wheeler occupants most commonly involved. Fissure fracture of the skull was commonest (45.71%) & parietal region of head was mostly involved region of the head (27.61%). Among the intracranial hemorrhages, subdural hemorrhage (SDH) was commonest (31.42%). In relation duration of survival time 59.04% of victims died within 24 hrs of fatal accident.
PERFORMANCE OF FREE LIVING N2-FIXERS BACTERIA, COMPOST TEA AND MINERAL NITROGEN APPLICATIONS ON SOME SOIL PROPERTIES, PRODUCTIVITY AND QUALITY OF ONION CROP (Giza red vr.).

A field experiment was carried out at the Experimental Farm of Sakha Agricultural Research Station in Kafr El-Sheikh Governorate at North Nile Delta Region. The experiments were conducted to study the response of onion to nitrogen fertilizer levels under different bio-organic fertilizers (compost tea and N2-fixing bacteria) as well as their interaction, on some soil properties and the growth and onion bulbs yield and its quality during the two successive winter seasons of 2009/2010 and 2010/2011. A split-split plot design with three replicates was used in this study. The main plots were designated to the three mineral nitrogen fertilizer levels (60, 90 and 120 kg N fad. -1 ), whereas foliar spraying treatments with compost tea at the same dose, 20 L fad. -1 (spraying with water; foliar spraying at 40 days after transplanting (DAT); foliar spraying at 40 and 60 DAT; foliar spraying at 40, 60 and 80 DAT and soil application, 30 DAT at rate of 30 L fad. -1 ) were randomly distributed in sub plots. While uninoculation and inoculation with di-nitrogen fixing bacteria (Azotobacter Chroococum and Azospirilium Brasilense) were randomly distributed in the sub-sub plots. The obtained results could be summarized as follow: The highest increase in soil available nutrients (N, P and K), organic matter percentages and total bacterial counts were increased significantly and recorded in the plots treated with compost tea as soil application batches and/or inoculation at nitrogen fertilizers level 90 kg N fed -1 (N2B4C2) while the lowest were recorded in the uninoculated plots treated with N fertilizers at 60 kg N fed -1 without both compost tea and bacterial inoculation. With inoculation and mineral nitrogen applications, soil salinity and pH were decreased in compost tea treatment under soil application as compared with others compost tea applications. The experimental results confirmed that the combination of compost tea, inoculation by di-nitrogen fixing bacteria and mineral nitrogen fertilizers could improve soil fertility. Growth and onion bulbs yield and their quality were highest due to application of compost tea on thrice than twice batches at the same dose and other treatments. The effect of bio-fertilizer in plant height, leaves dry weight/plant, bulb dry weight, plant dry weight, bulb diameter and bulb weight as well as bulb quality was evident in both seasons. In general, inoculation with Azotobacter chroococcum and Azospirilium brasilense positively increased all the previous characters. Based on the results of the current study, the combinations of medium dose of mineral N and compost tea or biofertilizers could be considered as an integrated nutrient management to improve soil bio-physical properties and the growth and yield of onion plants. It also confirmed that compost tea can be used as organic substrate additives in plant cultivation and substitute for chemical nitrogen fertilizers.
Modulation of Neurobehavioral and Biochemical Alterations Induced by Aluminum Chloride with Cannabis sativa Extract

Cannabis sativa preparations in the form of marijuana (the dried flowering tops and leaves) and hashish (the dried resin and compressed flowers) from the female plant Cannabis sativa is the most widely used illicit drugs worldwide. In humans, cannabis is associated with a spectrum of central nervous system effects. These include euphoria, relaxation and perceptual alterations, which are mediated by Δ9-tetrahydrocannabinol (Δ9-THC) the primary psychoactive constituent of cannabis. Other cannabinoids include cannabinol, cannabidiol, cannabigerol, cannabichromene, and cannabidivarin. Cannabinoid CB1 receptors have been cloned and identified in high concentration in several brain regions eg. , hippocampus, basal ganglia, cerebellum and cerebral cortex.
INFLUENCIA DE GOMA XANTAN Y GOMA GUAR SOBRE LAS PROPIEDADES REOLÓGICAS DE LECHE SABORIZADA CON COCOA

En las bebidas lacteas son utilizados diferentes hidrocoloides con el fin de estabilizarel sistema liquido, en este sentido es necesario determinar la interaccion entrealgunos hidrocoloides en la matriz alimenticia. El objetivo de este trabajo fueevaluar 3 estabilizantes: goma guar, goma xantan y carragenina kappa en lechesaborizada con cocoa alcalina. Inicialmente fue estimada la mezcla optima entreestos estabilizantes. La variable respuesta utilizada fue viscosidad, en la cual laevaluacion de tixotropia tuvo en cuenta los indices: adelgazamiento, recuperacion y tixotropia. Los analisis reologicos y fisicoquimicos fueron realizados en un periodo de dos semanas. La mejor mezcla de hidrocoloides fue 70% y 30% para goma xantan y goma guar, respectivamente. La concentracion ideal fue determinada bajo 3 tratamientos: T1:0,08%, T2:0,1% y T3:0,12% para el sistema de leche saborizada con cocoa. Los resultados del analisis reologico muestran diferencia significativa (p<0.05) entre los tratamientos utilizados. Los mejores resultados fisicoquimicos muestran que los valores de grasa, pH,sineresis y analisis sensorial fueron obtenidos para la concentracion 0,08%.
Distribution and association of factors influencing emerging maize lethal necrosis disease epidemics in Southern Ethiopia

Abstract Maize lethal necrosis (MLN) disease has emerged as a great threat of maize production in Ethiopia. Thus, field surveys were conducted to determine the spatial distribution and association of MLN disease with biophysical factors in Southern Ethiopia, during 2016 and 2017 main cropping seasons. A total of 240 maize fields were inspected across seven districts in the 2 years. Results showed that MLN disease was 100% prevalent in maize fields though the level of importance varied among districts. Arbaminch, Mihirab Arbaya, Bonkie, Derashie, Konso and Burjdi districts lowered disease severity by 1.46%, 4.13%, 11.38%, 13.40%, 9.24% and 30.82% when compared with Demba Gofa district, respectively. Also, severe weed and insect infestations were evident in the assessed farms. The association of disease severity with biophysical factors was assessed using a logistic regression model. District, NPS fertilization, cropping system, insect and weed infestation and insecticide spray frequency were significantly associated with disease severity in the multiple reduced variable model. High (>50%) disease severity had about four, three, and two times high probability of relationship with Demba Gofa, Arbaminch, and Mihirab Arbaya, Konso, Derashie and Bonkie districts, respectively. Similarly, 0 to 50 kg ha−1 NPS fertilization, sole cropping, high insect and weed infestation and none to once insecticide spray showed a high probability of association with disease severity. The results indicated that MLN disease is highly prevalent, severe and important in Southern Ethiopia. Therefore, weeding, insect vector control, NPS fertilization, intercropping, along with other agronomic practices, are recommended in the study areas and other related agro-ecologies.
Mixed PGPR consortium: an effective modulator of antioxidant network for management of collar rot in cauliflower

Abstract Sclerotinia sclerotiorum is a cosmopolitan fungal pathogen causing stem and collar watery soft rot of cauliflower. Previous management of this disease with chemical pesticides caused hazardous results which lead to use of more eco-friendly microbial approaches. In the present study, consortia of Trichoderma harzianum TNHU27 and Pseudomonas aeruginosa PJHU15 were assessed for their ability in controlling Sclerotinia rot in cauliflower. The seedlings of cauliflower were challenged singly and in consortium with these two compatible microbes. The plants were evaluated upon challenge inoculation with S. sclerotiorum for changes in total phenolic content, the activity of defense and antioxidative enzymes. The microbial consortium comprising of T. harzianum and P. aeruginosa significantly enhanced the defense responses of the plant in comparison to pathogen challenged and unchallenged control. The study elucidates that plant beneficial microbes in the consortium may provide superior protection by induction of faster and enhanced defense responses in comparison to unchallenged and single microbe challenged plants under pathogen challenged conditions.
Efficacy of an Intervention Based on the Theory of Planned Behavior on Foot Care Performance in Type II Diabetic Patients

Background: It is known that health education on foot care is a common strategy for preventing diabetic foot and reducing the rate of lower limb amputation. Objectives: To evaluate the efficacy of an intervention based on the theory of planned behavior for improving foot care in patients with type II diabetes in 2013 in Ahvaz, Iran. Patients and Methods: In this clinical trial, 69 patients (30 - 60 years old) who were referred to Golestan Hospital Diabetes Clinic between June and May 2013 were selected randomly and divided into 2 groups (intervention and control). The control group received the clinic’s routine training. In contrast, a 4-week educational program based on the theory of planned behavior was held for the intervention group. Data were collected before and after the intervention using a questionnaire developed for this study that assessed components of the theory of planned behavior, and foot care. Data were analyzed with SPSS version 19 using paired and independent t-tests and chi-squared. Results: The two groups showed no significant difference in terms of demographics or in the mean scores of other variables before the intervention (P > 0.05). After the intervention, a significant increase was observed in the mean scores of attitudes, and foot care performance in the intervention group, and the intervention group had higher scores than did the control group (P < 0.05). Conclusions: An intervention based on the theory of planned behavior was effective in improving the foot care in diabetic patients.
Matching cell lines with cancer type and subtype of origin via mutational, epigenomic and transcriptomic patterns

Cell lines are commonly used as cancer models. Because the tissue and/or cell type of origin provide important context for understanding mechanisms of cancer, we systematically examined whether cell lines exhibit features matching the cancer type that supposedly originated them. To this end, we aligned the mRNA expression and DNA methylation data between ∼9,000 solid tumors and ∼600 cell lines to remove the global differences stemming from growth in cell culture. Next, we created classification models for cancer type and subtype using tumor data, and applied them to cell line data. Overall, the transcriptomic and epigenomic classifiers consistently identified 35 cell lines which better matched a different tissue or cell type than the one the cell line was originally annotated with; we recommend caution in using these cell lines in experimental work. Six cell lines were identified as originating from the skin, of which five were further corroborated by the presence of a UV-like mutational signature in their genome, strongly suggesting mislabelling. Overall, genomic evidence additionally supports that 22 (3.6% of all considered) cell lines may be mislabelled because we predict they originate from a different tissue/cell type. Finally, we cataloged 366 cell lines in which both transcriptomic and epigenomic profiles strongly resemble the tumor type of origin, designating them as ‘golden set’ cell lines. We suggest these cell lines are better suited for experimental work that depends on tissue identity and propose tentative assignments to cancer subtypes. Finally, we show that accounting for the uncertain tissue-of-origin labels can change the interpretation of drug sensitivity and CRISPR genetic screening data. In particular, in brain, lung and pancreatic cancer cell lines, many novel determinants of drug sensitivity or resistance emerged by focussing on the cell lines that are best matched to the cancer type of interest.
Quantitative multiplexed proteomics of mouse microglia by flow-cytometric sorting reveals a core set of highly-abundant microglial proteins

Background Proteomic characterization of microglia has been limited by low yield and contamination by non-microglial proteins in magnetic-activated cell sorting (MACS) enrichment strategies. To determine whether a fluorescence-activated cell sorting (FACS)-based strategy overcomes these limitations, we compared microglial proteomes of MACS and FACS-isolated CD11b+ microglia in order to identify core sets of microglial proteins in adult mouse brain tissue. Results Quantitative multiplexed proteomics by tandem mass tag mass spectrometry (TMT-MS) of MACS-enriched (N = 5) and FACS-isolated (N = 5) adult wild-type CD11b+ microglia identified 1,791 proteins, of which 953 were differentially abundant, indicating significant differences between both approaches. While the FACS-isolated microglia proteome was enriched with cytosolic, endoplasmic reticulum and ribosomal proteins involved in protein metabolism and immune system functions, the MACS-enriched microglia proteome was enriched with proteins related to mitochondrial function and synaptic transmission. As compared to MACS, the FACS microglial proteome showed strong enrichment for canonical microglial proteins while neuron, astrocyte, and oligodendrocyte proteins were depleted. We identified a core set of proteins highly abundant in microglia including Msn and Cotl1 which were validated in immuno-histochemical studies. By comparing FACS-isolated microglia proteomes with transcriptomes, we observed highly concordant as well as highly discordant proteins that were abundant at the protein level but low at the transcript level. Conclusions We demonstrate that TMT-MS proteomics of FACS isolated adult mouse microglia is superior to column-based enrichment approaches, resulting in purer and more highly-enriched microglial proteomes. We also define core sets of highly-abundant adult microglial proteins that can guide future studies.
In vitro growth of ectomycorrhizal fungi associated with Pinus radiata plantations in Chile

A comparative study of in vitro growth of three species of ectomycorrhizal fungi (ECMF) (Rhizopogon luteolus, Suillus granulatus and Suillus luteus) was performed. Fungal material was collected in adult Pinus radiata plantations. Isolation and purification of the strains were performed on potato-dextrose-agar medium and the evaluation of the radial growth rate and the increase in mycelial biomass, under different culture conditions, was performed on the Modified Melin Norkrans growth medium. The effects of temperature (24, 28 and 32 oC) and pH (4.8, 5.3, 5.8, 6.3 and 6.8) of the growth medium were tested for the three fungal species in two independent assays. The results indicate that the temperature had a significant effect on the radial growth rate (RG) and mycelial biomass increase (MB) in all of the evaluated fungal species. The highest RGR and MBI were recorded in R. luteolus, and the lowest values for these variables were registered in S. luteus. Rhizopogon luteolus had the highest sensitivity to pH changes. Meanwhile, there was no pattern in S. granulatus and S. luteus growth response under different pH conditions. When cultivated in vitro, the three studied species of ECMF presented adaptation, exponential, declining and stationary growth phases. The in vitro growth responses recorded in the present study showed the great potential of R. luteolus to be used in future programs using mycorrhizal inoculation in the production of Pinus radiata trees in nurseries in Chile.
Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Transcriptional and Biochemical Function

Autoinflammatory disease can result from monogenic errors of immunity. We describe herein the first example of a patient with early-onset widespread autoinflammation resulting from a mosaic, heterozygous, gain-of-function mutation (S703I) in JAK1, encoding a kinase essential for signaling downstream of over twenty-five cytokines. By first-of-its-kind custom single-cell RNA sequencing, we examine mosaicism with single cell resolution. We uncover that JAK1 transcription is predominantly restricted to a single allele across different immune cells, introducing the concept of a mutational “transcriptotype” that differs from the genotype. Functionally, the S703I mutation not only increased JAK1 kinase activity, but also resulted in transactivation of partnering JAKs, independently of its catalytic domain. Further, S703I JAK1 was not solely hypermorphic for cytokine signaling, but neomorphic as well, as it enabled downstream signaling cascades not canonically mediated by JAK1. Given these results, the patient was treated with tofacitinib, a JAK inhibitor, which led to rapid resolution of her clinical disease. Together, these findings represent an unprecedented degree of personalized medicine with the concurrent discovery of fundamental biological principles.