Miracle-dz/UniFAR-FTUs huggingface dataset:
A smartphone microscopic method for simultaneous detection of (oo)cysts of Cryptosporidium and Giardia

Background Food and water-borne illness caused by ingestion of (oo)cysts of Cryptosporidium and Giardia is one of the major health problems globally. Several methods are available to detect Giardia cyst and Cryptosporidium oocyst in food and water. Most of the available methods require a good laboratory facility and well-trained manpower and are therefore costly. There is a need of affordable and reliable method that can be easily implemented in resource limited settings. Methodology/Principle findings We developed a smartphone based microscopic assay method to screen (oo)cysts of Cryptosporidium and Giardia contamination of vegetable and water samples. The method consisting of a ball lens of 1 mm diameter, white LED as illumination source and Lugols's iodine staining provided magnification and contrast capable of distinguishing (oo)cysts of Cryptosporidium and Giardia. The analytical performance of the method was tested by spike recovery experiments. The spike recovery experiments performed on cabbage, carrot, cucumber, radish, tomatoes, and water resulted in 26.8±10.3, 40.1±8.5, 44.4±7.3, 47.6±11.3, 49.2 ±10.9, and 30.2±7.9% recovery for Cryptosporidium, respectively and 10.2±4.0, 14.1±7.3, 24.2±12.1, 23.2±13.7, 17.1±13.9, and 37.6±2.4% recovery for Giardia, respectively. The spike recovery results are comparable with data obtained using commercial brightfield and fluorescence microscope methods. Finally, we tested the smartphone microscope system for detecting (oo)cysts on 7 types of vegetable (n = 196) and river water (n = 18) samples. Forty-two percent vegetable and thirty-nine percent water samples were found to be contaminated with Cryptosporidium oocyst. Similarly, thirty-one percent vegetable and thirty-three percent water samples were contaminated with Giardia cyst. Conclusions The newly developed smartphone microscopic method showed comparable performance to commercial microscopic methods. The new method can be a low-cost and easy to implement alternative method for simultaneous detection of (oo)cysts in vegetable and water samples in resource limited settings.
Effects of First Feed Administration on Small Intestinal Development and Plasma Hormones in Broiler Chicks

Simple Summary In this study, the effects of first feed administration on intestinal morphology, barrier function, and plasma hormones in broilers during the initial 168 h posthatch. Results revealed that early feeding posthatch had a positive effect on small intestinal growth by increasing weight and improving intestinal morphology and barrier function. In other words, early feeding promoted intestinal development, which could be very meaningful for commercial broiler production. Abstract (1) Background: Under practical conditions, newly hatched chicks were usually withheld feed and water for 48 to 72 h. It was shown that early feeding after hatch promoted gastrointestinal development of broiler chicks. However, the mechanism of early feeding affecting intestinal development in chicks needs further research. The present study was conducted to investigate the effects of first feed administration on intestinal morphology, barrier function, and plasma hormones in broilers during the initial 168 h posthatch. (2) Methods: A total of 720 one-day-old chicks (newborn chick, Lingnan Yellow) were placed 2 h after hatch and randomly assigned to three treatments: Group A (feed immediately after placement), Group B (fasting for 24 h after placement), and Group C (fasting for 48 h after placement). The trial lasted for 168 h and water ad libitum all the time. Sampling was performed at 0, 24, 48, 72, 120, and 168 h. (3) Results: Higher (p < 0.05) absolute weight and relative weight of the small intestine were observed in Group A. Moreover, the villus height, crypt depth, and ratio of the jejunum and ileum were significantly higher (p < 0.05) in Groups A and B than those in Group C. Microvilli of the duodenum were closely packed in Group A but sparse and disorganized in Groups B and C. The expression levels of mRNA and protein of tight junction genes (occludin and claudin-1) were upregulated (p < 0.05) in Group A. The levels of gastrin and insulin in plasma were decreased (p < 0.05) significantly in the Groups B and C. However, chicks in Groups B and C had higher (p < 0.05) plasma glucagon levels at 24 and 48 h after placement. (4) Conclusions: These results suggested that early feeding posthatch had a positive effect on small intestinal growth increasing weight and improving intestinal morphology and barrier function.
Occult Bacteremia in Young Children with Very High Fever Without a Source: A Multicenter Study

We carried out a prospective multicenter study including 203 previously healthy well-appearing children who were 3–24 months old with fever without a source ≥40.5°C. Thirty-one (15.3%, 95% confidence interval 11.0–20.9) were diagnosed with serious bacterial infection, including 6 with bacteremia (3%, 95% confidence interval 1.4–6.3). Testing for occult bacteremia in children 3–24 months old with fever without a source should be considered when fever at ≥40.5°C.
Maneuvers and strategies in respiratory physical therapy: time to revisit the evidence

1. Disciplina de Pneumologia, Universidade Federal de São Paulo – UNIFESP – São Paulo (SP) Brasil. 2. Serviço de Fisioterapia, Hospital São Paulo, Disciplina de Pneumologia, Universidade Federal de São Paulo – UNIFESP – São Paulo (SP) Brasil. 3. Hospital Vila Nova Star, São Paulo (SP) Brasil. 4. Universidade Nove de Julho, São Paulo (SP) Brasil. 5. Liga da Fisiointensiva, São Paulo (SP) Brasil. 6. Clinica Play Edu, São Paulo (SP) Brasil. 7. Programa de Mestrado e Doutorado, Universidade Cidade de São Paulo – UNICID – São Paulo (SP) Brasil. Respiratory physical therapy has always sought to prove the efficiency of techniques,(1-3) such as chest vibration/ percussion and changes in inspiratory/expiratory flow, in bronchial hygiene. However, this has failed to be proven because of methodological differences among the studies and the fact that these techniques depend on being properly performed by the physical therapist, as well as, in many cases, on the collaboration of the patients. (4) We believe that studies that corroborate the use of bronchial hygiene techniques are important, since they are necessary to prevent atelectasis and pulmonary infections, as well as to reduce the length of hospital stay. In this context, Chicayban et al.,(5) in the present issue of the Jornal Brasileiro de Pneumologia, contemplate us with a study comparing two bronchial hygiene techniques that can be used in clinical practice.
Cortical inexcitability defines an adverse clinical profile in amyotrophic lateral sclerosis

In amyotrophic lateral sclerosis, studies using threshold‐tracking transcranial magnetic stimulation (TMS) have identified corticomotoneuronal dysfunction as a key pathogenic mechanism. Some patients, however, display no motor response at maximal TMS intensities, termed here an ‘inexcitable’ motor cortex. The extent to which this cortical difference impacts clinical outcomes remains unclear. The aim of this study was to determine the clinical profile of patients with inexcitability to TMS.
Natural variation in autumn expression is the major adaptive determinant distinguishing Arabidopsis FLC haplotypes

In Arabidopsis thaliana, winter is registered during vernalization through the temperature-dependent repression and epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC). Natural Arabidopsis accessions show considerable variation in vernalization. However, which aspect of the FLC repression mechanism is most important for adaptation to different environments is unclear. By analysing FLC dynamics in natural variants and mutants throughout winter in three field sites, we find that autumnal FLC expression, rather than epigenetic silencing, is the major variable conferred by the distinct Arabidopsis FLChaplotypes. This variation influences flowering responses of Arabidopsis accessions resulting in an interplay between promotion and delay of flowering in different climates to balance survival and, through a post-vernalization effect, reproductive output. These data reveal how expression variation through non-coding cis variation at FLC has enabled Arabidopsis accessions to adapt to different climatic conditions and year-on-year fluctuations.
Evaluation of SWI/SNF Protein Expression by Immunohistochemistry in Ovarian Clear Cell Carcinoma.

Ovarian clear cell carcinomas (OCCC) are known to harbor ARID1A mutations, and several recent studies have described immunohistochemical loss of SMARCA2, SMARCA4, and SMARCB1 in a subset of tumors. We performed ARID1A, SMARCA2, SMARCA4, and SMARCB1 immunohistochemistry on 105 OCCCs to identify possible associations with clinicopathologic features and assess their prognostic value in these tumors. ARID1A, SMARCA4, and SMARCB1 were considered retained if any tumor cell nucleus stained while for SMARCA2, >5% of tumor nuclei were required to be positive. Patients had a mean age of 56 yr and tumors averaged 13 cm in size. Most patients (63%) had stage I tumors with 47% being alive and well, 41% dead from disease, 10% dead from other causes, and 3% alive with disease at last follow-up (mean 72 mo). Tumors showed an admixture of architectural patterns, but papillary was most frequent (49%). Stromal hyalinization was detected in 83% of OCCCs and a background precursor in 78%. High-grade atypia and/or oxyphilic cells were noted in 45% and 29% of tumors, respectively. All OCCCs expressed SMARCA4 and SMARCB1, but the absence of ARID1A was noted in 30% of tumors and SMARCA2 in 8%. ARID1A-retained OCCCs were associated with a dominant tubulocystic or solid pattern, but no other clinicopathologic features reached statistical significance. No switch/sucrose non-fermentable protein expression was predictive of prognosis. Additional studies with known mutational status of these proteins are warranted to better assess their prognostic utility and develop a standardized immunohistochemical scoring system.
Assessment of the Speed Management Impact on Road Traffic Safety on the Sections of Motorways and Expressways Using Simulation Methods

Methods used to evaluate the impact of Intelligent Transport System (ITS) services on road safety are usually based on expert assessments or statistical studies. However, commonly used methods are challenging to apply in the planning process of ITS services. This paper presents the methodology of research using surrogate safety measures calculated and calibrated with the use of simulation techniques and a driving simulator. This approach supports the choice of the type of ITS services that are beneficial for traffic efficiency and road safety. This paper presents results of research on the influence of selected scenarios of variable speed limits on the efficiency and safety of traffic on the sections of motorways and expressways in various traffic conditions. The driving simulator was used to estimate the efficiency of lane-keeping by the driver. The simulation traffic models were calibrated using driving simulator data and roadside sensor data. The traffic models made it possible to determine surrogate safety measures (number of conflicts and their severity) in selected scenarios of using ITS services. The presented studies confirmed the positive impact of Variable Speed Limits (VSLs) on the level of road safety and traffic efficiency. This paper also presents recommendations and plans for further research in this area.
Regardless of Age, Obesity and Hypertension Increase Risks With COVID-19.

Editor's Note Regardless of Age, Obesity and Hypertension Increase Risks With COVID-19 Older age has consistently been associated with higher mortality in patients with coronavirus disease 2019 (COVID-19). 1,2 Unfortunately, as shown by Cunningham et al3 in this issue of JAMA Internal Medicine, COVID-19 does not spare young people. Using a national all-payer hospital database, the investigators identified 3222 nonpregnant adults aged 18 to 34 years who were admitted to US hospitals for COVID-19. Morbidity was substantial: 21% required intensive care, and 2.7% died. Mortality was higher among those who had obesity, hypertension, and male sex, as has been noted in general adult populations. Combined with what we know about the greater risk of older persons, what does this study tell us about COVID-19 and young adults? First, while young adults are much less likely than older persons to become seriously ill, if they reach the point of hospitalization, their risks are substantial. Second, obesity, hypertension, and male sex put patients of all ages at greater risk. As obesity and hypertension are preventable and treatable conditions, reducing the risk of serious COVID 19 illness should be added to the already long list of reasons to increase medical and public health efforts in young adults to promote healthful diets and increased exercise. Finally, the article by Cunningham et al3 establishes that COVID-19 is a life-threatening disease in people of all ages and that social distancing, facial coverings, and other approaches to prevent transmission are as important in young adults as in older persons.
Leather Waste to Enhance Mechanical Performance of High-Density Polyethylene

Leather buffing dust (BF) is a waste from tannery which is usually disposed on landfills. The interest in using wastes as fillers or reinforcements for composites has raised recently due to environmental concerns. This study investigates the potential use of BF waste as filler for a high density polyethylene matrix (HDPE). A series of HDPE-BF composites, containing filler concentrations ranging from 20 to 50wt%, were formulated, injection molded and tested. The effect of filler contents on the mechanical properties of the composites were evaluated and discussed. Composites with BF contents up to 30wt% improved the tensile strength and Young’s modulus of the matrix, achieving similar mechanical properties to polypropylene (PP). In the case of flexural strength, it was found to be proportionally enhanced by increasing reinforcement content, maintaining high impact strength. These composites present great opportunities for PP application areas that require higher impact resistance. The materials were submitted to a series of closed-loop recycling cycles in order to assess their recyclability, being able to maintain better tensile strength than virgin HDPE after 5 cycles. The study develops new low-cost and sustainable composites by using a waste as composite filler.
Systematic relationships in Juniperus based on random amplified polymorphic DNAs (RAPDs).

44 taxa of Juniperus were analysed using RAPDs, similarity measures and principal coordinate analyses (PCO). The three sections of Juniperus (sect. Caryocedrus, sect. Juniperus, sect. Sabina) were found to be distinguished by RAPDs. The genus appears to be naturally divided into three major sections with two series (serrate and smooth leaf margins) in J. sect. Sabina. Additional examinations of specific and infraspecific taxa confirms earlier taxonomic work based on leaf terpenoids. Computer software (PCO3D from R.P.A.) for PCO is introduced for the analysis of RAPDs. RAPDs were found to be of taxonomic use ranging from sectional to varietal levels.
Clinical and Pathological Studies on Cattle Experimentally Infected with Theileria annulata in China

Theileriosis is an important tick-borne protozoosis that causes high morbidity and mortality in cattle. In this study, the pathological and clinical characteristics of cattle experimentally infected with Theileria annulata were investigated. The clinical findings revealed typical signs of bovine theileriosis, including fever, enlargement of superficial lymph nodes, anemia, and respiratory distress. The most common pathological features were petechial and ecchymotic hemorrhages on the mucosa and serosal surface, severe jaundice, pulmonary edema and emphysema, multifocal necrosis and numerous ulcerations in the abomasum, congestion and marble-like discoloration of the spleen, and severe intestinal ecchymotic hemorrhages. The main histological characteristics were proliferation and infiltration of lymphocytes, plasma cells, and macrophages in the lymph nodes, spleen, and lymph node mass. Macroschizonts were observed in the cytoplasm of lymphocytes and macrophages of the lymph nodes and spleen. This study has significance for basic research and the clinical detection and diagnosis of Theileria annulata infection and can aid the prevention and control of theileriosis and future studies of the pathogenic mechanisms.
Impact of Ozone, Sex, and Gonadal Hormones on Bronchoalveolar Lavage Characteristics and Survival in SP-A KO Mice Infected with Klebsiella pneumoniae

Surfactant protein A (SP-A) plays an important role in innate immunity. The sex-dependent survival of infected SP-A knockout (KO) mice has been observed. Our goal was to study the impact of ozone (O3) and sex, as well as gonadal hormones, on the bronchoalveolar lavage (BAL) readouts and survival, respectively, of Klebsiella pneumoniae-infected SP-A KO mice. Male and female SP-A KO mice were exposed to O3 or filtered air and infected with K. pneumoniae. We studied markers of inflammation and tissue damage at 4, 24, and 48 h, as well as the survival over 14 days, of gonadectomized (Gx) mice implanted with control pellets (CoP) or hormone (5α-dihydrotestosterone (DHT) in female gonadectomized mice (GxF) or 17β-estradiol (E2) in male gonadectomized mice (GxM)). We observed: (1) an increase in neutrophil and macrophage inflammatory protein-2 levels as time progressed post-infection, and O3 exposure appeared to increase this response; (2) an increase in lactate dehydrogenase, total protein, oxidized protein, and phospholipids in response to O3 with no consistent sex differences in studied parameters; and (3) a reduction in survival of the GxM and CoP mice, the GxM and E2 mice, and the GxF and DHT mice but not for the GxF and CoP mice after O3. Without SP-A, (a) sex was found to have a minimal impact on BAL cellular composition and tissue damage markers, and (b) the impact of gonadal hormones on survival was found to involve different mechanisms than in the presence of SP-A.
Attitudes and Behavior towards Interprofessional Collaboration among Healthcare Professionals in a Large Academic Medical Center

The increasing rates of comorbidities among patients and the complexity of care have warranted interprofessional collaboration (IPC) as an important component of the healthcare structure. An initial step towards assessing the effectiveness of collaboration requires the exploration of the attitudes and experience of healthcare professionals towards IPC. This online survey aimed to examine the attitudes of healthcare professionals working in a large public academic medical center toward IPC in patient care and the healthcare team, and their behavior and experience regarding IPC. The rankings, according to the perceived importance among the respondents, of the four Interprofessional Education Collaborative (IPEC) core competencies (values/ethics, roles/responsibilities, interprofessional communication, teams/teamwork) were assessed. There were strong but varying levels of consensus among healthcare professionals (N = 551) that IPC facilitates efficient patient care, improves patient problem-solving ability, and increases better clinical outcomes for patients. They acknowledged that IPC promotes mutual respect within the healthcare team and providers’ ability to make optimal patient care decisions. However, overall more than 35% of the respondents did not attend multidisciplinary education sessions (grand rounds, seminars, etc.), and about 23% did not participate in bedside patient care rounds. Interprofessional communication was ranked as the most important IPEC core competence. Although the attitude towards IPC among healthcare professionals is strongly positive, many healthcare professionals face challenges in participating in IPC. Institutional policies that facilitate interprofessional learning and interactions for this group of healthcare professionals should be formulated. Online distance learning and interactions, and simulation-enhanced interprofessional education, are options for addressing this barrier. Hospital administrators should facilitate conducive work environments that promote IPC, based on IPEC core competencies, and promote programs that address the challenges of IPC.
Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells

We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.
Factors Informing the Return of Adopted Dogs and Cats to an Animal Shelter

Simple Summary This study examined the reasons cats and dogs are returned following adoption at one shelter in Austin, TX, USA. The study found that dogs were most likely to be returned for behavioral issues while cats were most likely to be returned for personal reasons. The length of ownership before the pet was returned to the shelter varied substantially. These data can be used in future discussions of how to develop pet retention programs that address the factors informing returned adoptions. Abstract Although the adoption rate of dogs and cats from animal shelters has increased, a proportion of animals are returned to the shelter after they are adopted. The purpose of this study was to assess the factors informing the return of 102 dogs to an animal shelter over a four-month period, and the return of 72 cats to an animal shelter over a three-month period. Descriptive statistics revealed dogs are most commonly returned for behavior issues related to aggression (38.2%), and cats are most commonly returned due to the adopter’s personal reasons (56.9%). The results also indicated that more than half of the dogs (51.0%) and cats (57.0%) returned in this study were owned for more than 60 days. Further research is needed to compare the effectiveness of different pet retention programs in addressing the factors that inform returned adoptions.