Miracle-dz/UniFAR-FTUs huggingface dataset:
A comprehensive cell pro ling reveals new cell subtypes and immune dysfunction in peritoneal uid of endometriosis

Gen Zou Zhejiang University School of Medicine Women's Hospital Jianzhang Wang Zhejiang University School of Medicine Women's Hospital Xinxin Xu Zhejiang University School of Medicine Women's Hospital Ping Xu Zhejiang University School of Medicine Women's Hospital Libo Zhu Zhejiang University School of Medicine Women's Hospital Qin Yu Zhejiang University School of Medicine Women's Hospital Yangying Peng Zhejiang University School of Medicine Women's Hospital Xinyue Guo Zhejiang University School of Medicine Women's Hospital Tiantian Li Zhejiang University School of Medicine Women's Hospital Xinmei Zhang (  zhangxinm@zju.edu.cn ) Zhejiang University School of Medicine Women's Hospital https://orcid.org/0000-0001-6474-3477
The Large Clostridial Toxins from Clostridium sordellii and C. difﬁcile Repress Glucocorticoid Receptor Activity (cid:1)

We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difﬁcile . This was demonstrated using a transient transfection assay system for GR transactivation. We also report that C. sordellii lethal toxin inhibited GR function in an ex vivo assay, where toxin reduced the dexamethasone suppression of the proinﬂammatory cytokine tumor necrosis factor alpha (TNF- (cid:1) ). Furthermore, the glucocorticoid antagonist RU-486 in combination with C. sordellii lethal toxin additively prevented glucocorticoid suppression of TNF- (cid:1) . These ﬁndings corroborate the fact that GR is a target for the toxin and suggest a physiological role for toxin-associated GR repression in inﬂammation. Finally, we show that this repression is associated with toxins
Induced by an Intrauterine Inflammation PDE4 Inhibition Prevents Preterm Delivery

The aim of this study was to explore the anti-inﬂammatory properties of phosphodiesterase-4 (PDE4) inhibitors in vivo and their potential ability to prevent inﬂammation-induced preterm delivery. Indeed, intrauterine inﬂammation is the major etiology of very preterm delivery, the leading cause of neonatal mortality and morbidity. Intrauterine injection of Escherichia coli LPS in 15-day-pregnant mice induced an increase of PDE4 activity and PDE4B expression at the maternofetal interface, a rise of amniotic ﬂuid levels of TNF- (cid:1) , IL-1 (cid:2) , IL-6, and IL-10 and provoked massive preterm delivery and fetal demise. Selective PDE4 inhibition by rolipram prevented the rise in the proinﬂammatory cytokines. Following the nuclear translocation of the transcription factor NF (cid:3) B, as a marker of cellular activation after the inﬂammatory challenge, showed a time-dependent sequential activation of the gestational tissues, from the uterine mesometrial to the fetal compartment, particularly in the glycogen-trophoblastic cells of the placenta. This activation was disrupted by PDE4 inhibition, and inﬂammation-induced preterm delivery and fetal demise were prevented. PDE4 selective inhibitors may thus represent a novel effective treatment to delay inﬂammation-induced preterm delivery and to prevent adverse outcomes in infants. The Journal of Immunology, 2007, 178: 1115–1121.
Multi‐objective optimisation of electro–hydraulic braking system based on MOEA/D algorithm

The electro-hydraulic composite braking system of the electric vehicle can effectively collect the wasted energy by the regenerative braking to improve the endurance mileage. In this study, according to the characteristic of the electro-hydraulic composite braking system, the energy flow processes are analysed, which includes the energy recovery generated by motor regenerative braking and energy consumption of the hydraulic braking system, such as hydraulic pump, brake line and brake valve. Based on this, the brake sense, energy recovery and loss are proposed as the evaluation index, and their quantitative formula are derived. Taking the brake sense and energy as the optimisation objectives, and ECE regulations as the constraints, the parameters of electro-hydraulic composite braking system are optimised-based on a multi-objective evolutionary algorithm based on decomposition (MOEA/D). The simulation results show that the electro-hydraulic composite braking system optimised by the MOEA/D algorithm can decrease the energy loss and make the driver obtain a better brake sense, which improves the comprehensive performance of the system. The research of this study can provide a certain basis for the design and optimisation of electro-hydraulic composite braking system.
Efﬁcacy and safety of ibutilide vs. transoesophageal atrial pacing for the termination of type I atrial ﬂutter

Aims Comparing efﬁcacy and safety of ibutilide vs. transoesophageal atrial pacing (ATP) for the termination of type I atrial ﬂutter (AFL). Methods and results Eighty-seven patients affected by AFL lasting between 2 h and 30 days were randomized in two groups: Group 1 d i.v. ibutilide treatment, up to 2 mg, and Group 2 d ATP, with ‘‘burst’’ and ‘‘ramp’’ pacing protocols. Sinus rhythm was restored in 36/45 (80%) patients in Group 1 vs. 18/42 (43%) in Group 2 ( P ! 0 : 0005). In Group 1, mean AFL duration was 11.4 G 7.7 days in responders vs. 12.1 G 7.6 in non-responders ( P ¼ ns), while in Group 2 it was 2.7 G 1.4 vs. 14.2 G 5.4 days (responders vs. non-responders, respectively, P ! 0 : 0001); 30/36 (83%) responders in Group 1 had AFL O 48 h vs. 10/18 (56%) responders in Group 2 ( P ! 0 : 05). Non-sustained polymorphic ventricular tachycardia occurred in 2 patients in Group 1 vs. none in Group 2 ( P ¼ ns). It did not require any speciﬁc treatment except the interruption of ibutilide infusion. AFL termination, but ibutilide was more effective when the arrhythmia had lasted longer than 48 h.
Role of the Agonist Binding Site in Up-Regulation of Neuronal Nicotinic (cid:1) 4 (cid:2) 2 Receptors

Long-term exposure to nicotinic ligands results in up-regulation of neuronal nicotinic (cid:1) 4 (cid:2) 2 receptors in the brain and in heter-ologous expression systems. Up-regulation can be produced by a variety of drugs, including nicotinic agonists and competitive antagonists, but previous studies have indicated that the signal for up-regulation does not reflect a traditional nicotinic pharmacology, and the initial steps in signal transduction are not clear. We examined the signal for up-regulation and the possible involvement of the nicotine binding site in signal re- ception using mutated subunits transiently expressed in human embryonic kidney 293 cells. The data indicate that receptor activation and desensitization are not part of the signaling pathway. However, mutations to residues in the binding site can affect up-regulation. These results provide evidence that the binding site is directly involved in receiving the signal for up-regulation. post hoc Systat of mutated
Preparation of Modiﬁed Colloidal Gas Aphrons and Analysis of the Oil Displacement E ﬀ ect

: Colloidal gas aphrons (CGAs) o ﬀ er some advantages in improving oil recovery, but resin and asphaltene deposition problems still occur in CGA ﬂooding. Based on this phenomenon, a new modiﬁed colloidal foam system is developed by incorporating a modiﬁer in CGA preparation. The results indicate that the modiﬁed CGAs prepared by adding foaming agent sodium dodecyl sulfate (SDS) (concentration: 5 g / L) and GXJ-C (a CGAs modiﬁer from a light fraction of petroleum; concentration: 0.1 g / L) attained the best performance. Oil displacement experiments show that modiﬁed CGA ﬂooding had a better e ﬀ ect than water or CGA ﬂooding. There are two important mechanisms via which modiﬁed CGAs enhance oil recovery, including decreasing the interfacial tension and enhancing the heavy components in the recovered oil. The developed modiﬁed CGA system attained a good oil displacement e ﬀ ect, which is of guiding signiﬁcance to further improve the oil displacement e ﬃ ciency and application of foam ﬂooding. The results showed that the stirring time, speed, and temperature had a large inﬂuence on the foaming properties of the modiﬁed CGAs, but the inﬂuence rules were di ﬀ erent. When the stirring time was 3 min, the modiﬁed CGA foaming volume and half-life period basically reached the equilibrium state. Within the experimental investigation range, a higher stirring speed led to a larger foam volume and a longer half-life period. When the stirring speed was 9000 rpm, the modiﬁed CGA foaming volume basically no longer increased, and the half-life period was no longer extended. When the preparation temperature increased from 15 ◦ C to 35 ◦ C, the modiﬁed CGA foaming volume decreased by 40%. For the same half-life period, the modiﬁed CGA foaming volume was only 50% of that at a preparation temperature of 15 ◦ C. When the temperature increased, the modiﬁed CGA foam volume tended to remain stable with the half-life period.
LICORICE AMELIORATES CADMIUM CHLORIDE INDUCED HEPATOTOXICITY IN ALBINO RATS VIA B-CELL LYMPHOMA 2 UP REGULATION

Cadmium (Cd) is a widespread toxic pollutant of occupational and environmental concern because of its diverse toxic effects including hepatotoxicity, nephrotoxicity and reproductive toxicities. There is an increasing demand for herbal medicines. Licorice is a plant used in traditional medicine across the world effectively used as an antioxidant. Aim: Investigate the protective role of licorice against cadmium chloride (CdCl2) induced hepatotoxicity. Methods: Forty male albino rats were randomly divided into four groups each consisting of 10 animals and were treated as follows: Untreated control group (G1), Licorice treated group (300mg/ kg b.w) intragastric daily for 21 days (G2), CdCl2 treated group (3mg/kg b.w) intraperitoneal 5 times per week for 3 weeks (G3) and a group treated with the licorice (300mg/ kg b.w) intragastric for 3days before CdCl2 injection and continued along with CdCl2 injection (3 mg/kg b.w) 5 times per week for 3 weeks (G4). Specimens from liver were taken and examined histopathologically besides estimation of hepatic enzymatic functions. Also, immunohistochemical expression of B-cell lymphoma 2 (Bcl-2) in the livers of all experimental rats was investigated. Results: Significant increase of hepatic enzymes levels in Cd treated rats when compared with control group with low levels of albumin and total protein. While, co-treatment of CdCl2 with licorice reduces the elevation of enzymatic activities. Histopathologically, the liver sections from Cd group showed severe changes including marked degenerative changes of hepatic cells, dark stained nuclei with condensed cytoplasm indicating nuclear pyknosis accompanied with marked kupffer cells proliferation as well as focal areas of hepatic necrosis with mononuclear cells infiltration. Combined administration of Licorice with CdCl2 improves the biochemical and histopathological changes induced by cadmium intoxication. Down-regulation of Bcl-2 in hepatic cells induced by Cd was restored by co-administration of licorice. Conclusion: Licorice has a protective effect against hepatotoxicity of cadmium.
An Ethnographic Study of Traditional Postpartum Beliefs and Practices among Chinese Women

: In many Asian societies, traditional postpartum practices are believed to be vital to the health of women and babies. Deep cultural and social meanings are attached to practices related to behaviors, activities, foods, hygiene, and infant care with variance by regions. Previous studies have demonstrated diverse interpretations of the traditional postpartum beliefs and practices by local communities. In the rural area of Jiujiang Jiangxi Province, China, postpartum women’s beliefs and practices have not been documented. This paper is part of an ethnographic study aimed to describe the traditional postpartum beliefs and practices among women in the sociocultural context of the rural area of Jiujiang, Jiangxi province. The villages of two townships of Maying and Zhangqing in Jiujiang City, were the settings for the study. Data collection was from January 2017 to July 2017 . Participant observation in the rural area context and in-depth interviews were conducted with 18 postpartum women as key informants. A six-step qualitative data analysis method was used. Three themes emerged from the traditional postpartum practices - zuo fang (sitting in room): gaining yin-yang (shady-sunny) balance, preventing ‘xie’ (pathogenic factor) entering the body, and enhancing breastmilk production. Variations of the traditional postpartum beliefs and practices were influenced by the local sociocultural context. Knowledge of the cultural importance of traditional postpartum beliefs and practices of rural women is required for nurses to provide culturally sensitive postpartum nursing care. Pacific Rim Int J Nurs Res 2019; 23(2) 142-155
Glomerular epithelial cell injury associated with mutant (cid:1) -actinin-4

Glomerular epithelial cell injury associated with mutant (cid:1) -actinin-4. Am 2009. Focal segmental glomerulosclerosis (FSGS) may be associated with glomerular epithelial cell (GEC; podocyte) apoptosis due to acquired injury or mutations in (cid:1) -actinin-4. This study addresses how FSGS-associated mutant (cid:1) -actinin-4 may induce GEC injury, focusing on endoplasmic reticulum (ER) stress and metabolism of mutant (cid:1) -acti-nin-4 via the ubiquitin-proteasome system. In a model of experimental FSGS induced by expression of an (cid:1) -actinin-4 K256E transgene in podocytes, we show induction of ER stress, including upregulation of ER chaperones (bip, grp94), phosphorylation of the eukaryotic translation initiation factor-2 (cid:1) subunit, and induction of the proapoptotic gene C/EBP homologous protein-10 (CHOP). To address mechanisms of ER stress, we studied signaling in cultured GEC and COS cells expressing (cid:1) -actinin-4 K256E. Previously, we showed that expression of this (cid:1) -actinin-4 mutant in GEC increased apoptosis. In the present study, we show that (cid:1) -actinin-4 K256E upregulates grp94 and CHOP expression in COS cells and signiﬁcantly exacerbates induction of bip and CHOP in GEC in the presence of tunicamycin. ER stress was associated with aggregation and ubiquitination of (cid:1) -actinin-4 K256E and impairment of the ubiquitin-proteasome system. In addition, (cid:1) -actinin-4 K256E exacerbated apoptosis in the context of mild proteasome inhibition. Thus (cid:1) -actinin-4 K256E triggers several met-abolic abnormalities, which may lead to GEC injury and glomerulosclerosis.
Reduced (cid:1) -cell mass and altered glucose sensing impair insulin-secretory function in (cid:1) IRKO mice

Reduced (cid:1) -cell mass and altered glucose sensing impair insulin-secretory function in (cid:1) IRKO mice. Am J Physiol Endocrinol Metab E41–E49, 2004. (cid:1) -cell-restricted knockout of the insulin receptor results in hyperglycemia due to impaired insulin secretion, suggesting that this cell is an important target of insulin action. The present studies were undertaken in (cid:1) -cell insulin receptor knockout ( (cid:1) IRKO) mice to deﬁne the mechanisms underlying the defect in insulin secretion. On the basis of responses to intraperitoneal glucose, (cid:2) 7-mo-old (cid:1) IRKO mice were either diabetic (25%) or normally glucose tolerant (75%). Total insulin content was profoundly reduced in pancreata of mutant mice compared with controls. Both groups also exhibited reduced (cid:1) -cell mass and islet number. However, insulin mRNA and protein were similar in islets of diabetic and normoglycemic (cid:1) IRKO mice compared with controls. Insulin secretion in response to insulin secretagogues from the isolated perfused pancreas was markedly reduced in the diabetic (cid:1) IRKOs and to a lesser degree in the nondiabetic (cid:1) IRKO group. Pancreatic islets of nondiabetic (cid:1) IRKO animals also exhibited defects in glyceraldehyde- and KCl-stimulated insulin release that were milder than in the diabetic animals. Gene expression analysis of islets revealed a modest reduction of GLUT2 and glucokinase gene expression in both the nondiabetic and diabetic mutants. Taken together, these data indicate that loss of functional receptors for insulin in (cid:1) -cells leads primarily to profound defects in postnatal (cid:1) -cell growth. In addition, altered glucose sensing may also contribute to defective insulin secretion in mutant animals that develop diabetes.
Eggplant Calyx the Newest Alternative Cure for Leishmaniasis Disease

For centuries, extracts from plants have been used as folk remedies against various health problems [1]. One of the important plants and herbal medicines is eggplant plant, which has been studied extensively, but its cones wasn’t studied thoroughly until now. This study is concerned with the detection and calibration of phenols in the eggplant cones. All the necessary reagents for detection of tannins, flavonoids and chlorogenic acid were positive, and then calibrated phenols with various solvent showed that the ethanolic aqueous extract has the highest percentage of phenol, followed by the aqueous then methanolic extracts. The properties of this extract in terms of its effects on the inhibition of Leishmania parasites were studied. The ethanolic aqueous extract showed a high measure in the rate of inhibition and is equivalent to IC50=39 µg/ml in vitro, the extract was formulated with cream and applied to randomly treated leishmaniasis patients in a double-blind study. The cases were studied in three groups without injection - with injection of glucantime – with liquid nitrogen treatment. Most of the cases have shown improvement or acceleration of improvement since the first week of treatment, confirming the effectiveness and importance of this plant section, which is usually wasted, that is eggplant cones as a new and undiscovered drug with a prospect to treat leishmaniasis. This study highlighted a new plant extract from eggplant calyx that has proven effective against leishmaniosis in vitro by achieving IC50 =39 µg/ml and the detection of some active substances in this wasted part of this plant, Eggplant (Solanum melongena L), which is a vegetable crop, economically important, consumed widely in Syria. It is native in South East Asian region and was domesticated over 4000 years ago [11]. Many studies have proved the effectiveness of this fruit in the treatment of many diseases such as diabetes, gonorrhoea, cholera, bronchitis, dyspnea, dysentery, debilitation and the treatment of hemorrhoids thus, this extract were either completely effective treatment or effective adjuvant treatment.
Exploring Entropy in Software Systems: Towards a Precise Deﬁnition and Design Rules

—Software systems need to be agile in order to continuously adapt to changing business requirements. Nevertheless, many organizations report difﬁculties while trying to adapt their software applications. Normalized Systems (NS) theory has previously been able to introduce a proven degree of evolvable modularity into software systems, based on the systems theoretic notion of stability. In this paper, we explore the applicability of this other fundamental property of systems (i.e., entropy) to the issues of software maintenance and evolvability. The underlying concepts in entropy deﬁnitions will be explained and applied to software systems and architectures. Further, the considerable complexity of running multi-tier multi-threading software systems and the relation with entropy concepts is discussed and illustrated. Finally, the concordance of design rules for controlling that entropy with previously formulated NS principles is explored.
Investigating escape from adaptive conflict in the legume Medicago truncatula

Trade-offs between genetically correlated traits cause significant evolutionary consequences. One example of an agriculturally and ecologically important trade-off exists between the ability for the legume plant Medicago truncatula to attract nitrogen-fixing rhizobia and to repel parasitic root-knot nematodes. In this study, I conducted a greenhouse experiment to examine one Medicago truncatula genotype that appears to have escaped this genetic conflict. I also included 17 closely grouped genotypes (based on phylogenetic distance) to see if I could identify others that behave similarly. While I was not able to replicate a positive correlation between susceptibility to rhizobia and nematodes, I did confirm the phenotype of the previously identified outlier genotype. Finally, I identified one other genotype that formed significantly more interactions with beneficial rhizobia while forming significantly less interactions with parasitic nematodes. My findings provide some very exciting avenues to pursue future research in identifying the genetic underpinnings of this interesting phenotype.
anti-inflammatory nutrients obesity-associated metabolic-inflammation from childhood through to adulthood.

Obesity-related metabolic conditions such as insulin resistance (IR), type 2 diabetes and CVD share a number of pathological features, one of which is metabolic-in ﬂ ammation. Metabolic-in ﬂ ammation results from the in ﬁ ltration of immune cells into the adipose tissue, driving a pro-in ﬂ ammatory environment, which can induce IR. Furthermore, resolution of in ﬂ ammation, an active process wherein the immune system counteracts pro-in ﬂ ammatory states, may be dysregulated in obesity. Anti-in ﬂ ammatory nutritional interventions have fo-cused on attenuating this pro-in ﬂ ammatory environment. Furthermore, with inherent variability among individuals, establishing at-risk populations who respond favourably to nutritional intervention strategies is important. This review will focus on chronic low-grade metabolic-in ﬂ ammation, resolution of in ﬂ ammation and the putative role anti-in ﬂ ammatory nutrients have as a potential therapy. Finally, in the context of personalised nutrition, the approaches used in de ﬁ ning individuals who respond favourably to nutritional
A Comparison of Nutrient and Dietary Compositions of Cereals and Pulses Commonly Consumed in Pakistan

The present study was taken to evaluate the proximate content and dietary fibre composition of locally available cereal (wheat, maize, oat and barley) and the legumes (mash beans, lentils, mung beans and chickpea). In cereal samples, crude proteins in all cereals were found in the range of 8.75-10.93% but in legumes this range was significantly higher i.e. (19.91-22.06). Crude fibre analysis in cereal samples showed values between 1.89-10.6, but in legume samples it ranged between 2.64 to 4.41. Total dietary fibre was higher in oat and barley 19.0 and 18.34, respectively, whereas total dietary fibre contents in selected legumes ranged between 18.00 (chick pea) to 24.93 (mung bean).