Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytometric analysis of T cell phenotype using cytokine profiling for improved manufacturing of an EBV‐specific T cell therapy

Adoptive immunotherapy using Epstein–Barr Virus (EBV)‐specific T cells is a potentially curative treatment for patients with EBV‐related malignancies where other clinical options have proved ineffective. We describe improved good manufacturing practice (GMP)‐compliant culture and analysis processes for conventional lymphoblastoid cell line (LCL)‐driven EBV‐specific T cell manufacture, and describe an improved phenotyping approach for analysing T cell products. We optimized the current LCL‐mediated clinical manufacture of EBV‐specific T cells to establish an improved process using xenoprotein‐free GMP‐compliant reagents throughout, and compared resulting products with our previous banked T cell clinical therapy. We assessed effects of changes to LCL:T cell ratio in T cell expansion, and developed a robust flow cytometric marker panel covering T cell memory, activation, differentiation and intracellular cytokine release to characterize T cells more effectively. These data were analysed using a t‐stochastic neighbour embedding (t‐SNE) algorithm. The optimized GMP‐compliant process resulted in reduced cell processing time and improved retention and expansion of central memory T cells. Multi‐parameter flow cytometry determined the optimal protocol for LCL stimulation and expansion of T cells and demonstrated that cytokine profiling using interleukin (IL)‐2, tumour necrosis factor (TNF)‐α and interferon (IFN)‐γ was able to determine the differentiation status of T cells throughout culture and in the final product. We show that fully GMP‐compliant closed‐process culture of LCL‐mediated EBV‐specific T cells is feasible, and profiling of T cells through cytokine expression gives improved characterization of start material, in‐process culture conditions and final product. Visualization of the complex multi‐parameter flow cytometric data can be simplified using t‐SNE analysis.
Development and Characterization of a Novel Vesicular System for TopicalDelivery: An Opportunity for Dimethylsulfoxide

Current use of Dimethylsulfoxide (DMSO) in dermatology and in topical formulations is scarce despite its potential as a skin penetration enhancer and as a pharmacological active itself. The aim of this work is to develop and characterize a new type of semisolid formulation of Soybean Phosphatidylcholine (SPC) - DMSO deformable nanovesicles for topical application. Tretinoin is used in this work as a model substance. The formulation presents a non-newtonian pseudoplastic behaviour and a pH compatible with the skin. Stability-on-storage at different temperatures indicate an excellent physical and chemical stability and SPC-DMSO vesicles show high deformability when forced to pass through pores much smaller than their own size. These new nanovesicles show a high Tretinoin yield. Calorimetric analysis indicates a possible interaction between Tretinoin and DMSO in the lipid vesicle bilayer and vesicles formulation exhibit low cytotoxic effect below 1.25% SPC. Moreover, these vesicles once applied on the skin do not cause irritation. This study presents a new opportunity in dermal delivery for a controversial molecule such as DMSO. SPC-DMSO vesicles have plenty of advantages for topical application and future studies should confirm their superiority as drug carriers for topical application as well as for clinical evaluations in managing skin disorders.
Effects of uric acid-lowering therapy in patients with essential arterial hypertension

Objectives Asymptomatic hyperuricemia (AHU) is elevated serum uric acid (UA) without symptoms. This study aimed to determine the effects of AHU treatment with allopurinol on selected hypertension-mediated organ damage (HMOD) indices in patients with uncomplicated essential arterial hypertension (AH). Methods Patients aged 30–70 years with AHU and AH grade 1–2 with adequate blood pressure (BP) control, without previous urate-lowering therapy (ULT), were divided into two groups: (a) ULT (receiving allopurinol) and (b) control (age- and sex-matched patients without ULT). Both received a UA-lowering diet. BP (office, 24 h and central), echocardiographic parameters, carotid intima-media thickness (IMT) and lab tests [high-sensitivity C-reactive protein (hs-CRP)] were measured at baseline and at 6 months follow-up. Results Of 100 participants, 87 (44 ULT, 43 controls) completed the study. At 6 months follow-up, there was a greater reduction in serum UA concentration in the ULT group than in the control group. Patients receiving allopurinol had significant reductions in office systolic and diastolic BP, central systolic BP, pulse pressure, IMT (0.773 ± 0.121 vs. 0.752 ± 0.13 mm, P = 0.044) and hs-CRP (3.36 ± 2.73 vs. 2.74 ± 1.91 mg/L, P = 0.028) compared to controls. Multivariate regression analysis revealed the independent relationship between reduction in IMT and UA lowering (P < 0.026). Conclusion In patients with AH and AHU, treatment with allopurinol leads to improvement in BP control and reduction in HMOD intensity, in particular IMT. The decrease in hs-CRP concentration associated with ULT may have a beneficial effect on a patient’s long-term prognosis.
Macro and micromineral in commercial infant formula milk in Indonesia by neutron activation analysis

The content of protein and minerals in infants formula milk is composed based on the composition of breast milk that provides adequate nutritional requirements for food intake in infants. Minerals as in macro and microminerals are essential for biological processes since they involve bone mineralization, enzymatic reactions, secretion of natural hormones, cells and lipid protection. The composition of macro and micromineral is one indicator in influential the quality of formula milk. Therefore, in this activity, the determination of macro and micromineral elements in infant formula milk was carried out by Neutron Activation Analysis (NAA) method and evaluated formula milk by considering the concentration of daily nutritional requirements. Formula milk samples were obtained in Indonesian market. The samples were irradiated on a lazy susan system at TRIGA 2000 Bandung Reactor with a neutron flux of ˜ 1013 n.cm−2.s−1 for three days. The validation method was also carried out using Typical Diet standard reference material. The validation results have a good agreement with the certificate value with bias (%) ranging from 0.6 to 6.9. Determination of macro and micromineral nutrients using NAA method resulted K, Na, Fe, Se, and Zn with an average value were 0.66%; 0.25%; 82.7 µg/g; 0.14 µg/g; and 34.7 µg/g respectively. These values were still within the concentration ranges as stated in nutritional labelled by manufacturers. The daily intake of macro and micromineral K, Na, Fe, Se, and Zn were 5150, 5110, 65, 0.11, and 27 mg/day, respectively. By consuming tolerable amounts of infant formula milk, this commodity is a source of nutrition that required by the infant’s nutritional adequacy rate.
The Cleaning of Silver Objects With a Basic Solution of Sodium Glycinate: A Study on Artificially and Naturally Tarnished Silver

ABSTRACT The appearance of silver objects deteriorates due to the formation of a brown-to-black tarnish layer. Several methods are available for removing these tarnished layers, ranging from polishing through chemical cleaning to electrochemical methods. This study presents a low-cost and low-toxicity method that uses sodium glycinate to clean silver. Cleaning tests were performed on both artificially tarnished prototypes and naturally tarnished objects after characterizing them; scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDX) were used to characterize the artificially tarnished prototypes, and X-ray fluorescence (XRF) was used to characterize the naturally tarnished objects. The aggressiveness of the baths was analyzed by measuring the leached amount obtained through atomic absorption spectroscopy (AAS) and SEM. The procedures used for cleaning the artificially tarnished prototypes served as a guide for cleaning two naturally tarnished objects: a halo and a crown. Cleaning was guided by the presented method’s characteristics as well as the object’s unique characteristics and condition.
Development and performance evaluation of a machine vision system and an integrated prototype for automated green shoot thinning in vineyards

Green shoot thinning in vineyards is an essential, perennial operation for maintaining canopy health and optimizing yield and quality of wine grapes. Use of mechanized thinning system, which is essential to reduce labor dependency and associated cost, causes high variability in shoot removal efficiency due to difficulty in precisely positioning the thinning end‐effector along cordon trajectories. Automated/robotic solution for precise positioning of the thinning end‐effector could significantly improve the performance and efficiency of mechanical green shoot thinning. This study presents: (i) a machine vision‐based cordon detection system that can estimate cordon trajectories at different shoot growth stages in a vineyard; and (ii) evaluation of an integrated green shoot thinning system capable of automatically positioning the thinning end‐effector following vine cordon trajectories. The developed machine vision system uses deep learning‐based techniques that could accurately estimate cordon trajectories with root mean square error (RMSE) of 7.3, 10.3, and 16.1 pixels for canopy images captured in 2–4 weeks of shoot growth, respectively. Then, a control strategy was presented for the integrated system, which receives the computed cordon trajectories from machine vision system to automatically position the thinning end‐effector to cordon trajectories. Field evaluations in a research vineyard showed that the integrated system can achieve an RMSE of 1.47 cm in following the cordon trajectories at 6.6 cm·s−1 forward speed. Future work will incorporate additional sensing system to detect individual shoot on cordon and integrating it with an existing system to achieve higher level of precision in green shoot thinning.
Freshwater jellyfish in northern temperate lakes: Craspedacusta sowerbii in British Columbia, Canada

Freshwater jellyfish species of the genus Craspedacusta purportedly originated from the Yangtze River catchment area, China, and have now been observed on all continents except Antarctica. Sightings of C. sowerbii in the Pacific regions of Canada were compiled to document some of the northernmost records of this species in the Americas. Species identification has been difficult in the past. Therefore, field collection of specimens was carried out on southern Vancouver Island. The morphology was described macroand microscopically as well as molecularly using mitochondrial and nuclear markers. Collected hydromedusae from British Columbia (BC) did not deviate morphologically from C. sowerbii specimens from other continents, but molecular analyses support the idea of 2 main widely distributed lineages hidden under similar morphological features (i.e. a species complex). Through a community science approach, an understanding of the extent of C. sowerbii distribution in western and southern BC (present in 24 lakes from as early as 1990) has been established. Results showed that the number of sightings increased considerably in the period after 2010. Recent increases in sightings of C. sowerbii in BC and worldwide could be indicative of a climate warming-related range extension or growing public awareness and/or increased observational efforts. Even after more than 120 yr of Craspedacusta research, much about their biology and ecology remains unknown, which motivated us to compile a list of knowledge gaps based on an extensive literature survey.
The Coronal Pulp Cavity Index an aid in age determination -A Cone Beam Computed Tomography Study

Background: The objective of the present study was to assess the accuracy of age estimation from TCI ofmandibular molars (except wisdom teeth) of both sides using CBCT images of known age and sex, so thatwe can apply this method to estimate age in both living individuals and skeletal material of unknown age.Methods: A cross sectional retrospective study was conducted on a total of 100 CBCT images of subjectswho were referred to the department for varied diagnostic purposes of known age and gender. All CBCTimages with a fully visible pulp cavity were selected. Two measurements were taken at two different levels.Height of the crown (CH) and height of the coronal pulp cavity (CPCH). Then tooth – coronal index (TCI)for each tooth was calculated as follows: TCI = CPCH X 100 / CH.Results: Comparison of TCI value between male and female showed no significant difference between thegender. Among the four mandibular molars (lower left and right1stand 2nd molars) 46 and 47 TCI correlateswell with age. TCI of 46 has significant prediction of age in female with an R value of 0.426. Inter observermeasurements showed a moderate to good agreement of the measures.Conclusions: TCI method of mandibular teeth was found to be a reliable method for age estimation and notgender determination. CBCT can be used in age determination for forensic purposes as it is non- invasiveand also makes it possible to reconstruct the images in different planes showing the anatomical and imagedstructures at different planes.
FABRICATION OF NANO CLAY INTERCALATED POLYMERIC MICROBEADS FOR CONTROLLED RELEASE OF CURCUMIN

Objective: The objective of this study was to formulate and evaluate the Curcumin (CUR) encapsulated sodium alginate (SA)/badam gum (BG)/kaolin (KA) microbeads for controlled drug release studies. Methods: The fabricated microbeads were characterized by fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (X-RD), and scanning electron microscopy (SEM). Dynamic swelling studies and in vitro release kinetics were performed in simulated intestinal fluid (pH 7.4) and simulated gastric fluid (pH 1.2) at 37 °C. Results: FTIR confirms the formation of microbeads. DSC studies confirm the polymorphism of CUR in drug loaded microbeads which indicate the molecular level dispersion of the drug in the microbeads. SEM studies confirmed the microbeads are spherical in shape with wrinkled and rough surfaces. XRD studies reveal the molecular dispersion of CUR and the presence of KA in the developed microbeads. In vitro release studies and swelling studies depend on the pH of test media, which might be suitable for intestinal drug delivery. The % of drug release values fit into the Korsmeyer-Peppas equation and n values are obtained in the range of 0.577-0.664, which indicates that the developed microbeads follow the non-Fickian diffusion drug release mechanism. Conclusion: The results concluded that the CUR encapsulated microbeads are potentially good carriers for controlled drug release studies.
A Novel Saturated Amorphous Alloy Core Based Fault Current Limiter for Improving the Low Voltage Ride Through Capability of Doubly-Fed Induction Generator Based Wind Turbines

This article proposes the use of a novel saturated amorphous alloy core-based fault current limiter (SAACFCL) to improve the low voltage ride through (LVRT) capability of doubly-fed induction generator (DFIG) based wind energy systems, especially during voltage sag events. Compared to the traditional cores, which are widely used in fault current limiter (FCL), amorphous alloy core possesses a very narrow B-H loop, which indicates that the SAACFCL requires a smaller dc excitation current, and it will incur low core losses. Under normal conditions, this developed SAACFCL accomplishes low impedance and has a negligible impact on the network operation. During grid faults, a deep voltage sag causes large fault currents that desaturate the SAACFCL core, increasing its impedance, which limits the fault currents and improves the LVRT capability of DFIG systems. The SAACFCL is designed and characterized by ANSYS software. To validate the performance of the SAACFCL, a DFIG-based system equipped with the SAACFCL has been modeled in MATLAB/Simulink. The simulation results with and without the SAACFCL show that the SAACFCL is capable to achieve the LVRT successfully and can meet the grid code requirement. The performance of the SAACFCL has also been compared with that of the saturated iron core FCL (SICFCL) and superconducting FCL (SFCL). The SAACFCL promises better performances than SICFCL and SFCL. A small scale SAACFCL has been designed, developed, and tested in the laboratory to check its performance. The experimental results show that the proposed SAACFCL can effectively reduce the level of fault current and mitigate voltage sag experienced by the DFIG.
Charismatic leadership and millennial employee innovation performance relationship mediated by employees' leadership, professional, and organizational identification

Using leadership behavior and social identity theories, we conducted an empirical study with 361 millennial employees to explore the mechanisms underlying the impact of charismatic leadership on employee innovation performance in the Chinese context, and to understand the serial mediation effects of employees’ leadership, professional, and organizational identification. The results show that charismatic leadership had a significant positive effect on millennial employee innovation performance and that this relationship was partially mediated by employees’ leadership, professional, and organizational identification. Moreover, a serial mediation effect was found via employees’ leadership and professional identification; leadership and organizational identification; professional and organizational identification; and leadership, professional, and organizational identification. The findings offer a new paradigm to explain the mechanisms through which charismatic leadership affects millennial employee innovation performance. Theoretical and practical implications are discussed.
Entrainment and Its Dependency on Environmental Conditions and Convective Organization in Convection-Permitting Simulations

In this study, we estimate bulk entrainment rates for deep convection in convection-permitting simulations, conducted over the tropical Atlantic Ocean, encompassing parts of Africa and South America. We find that, even though entrainment rates decrease with height in all regions, they are, when averaging between 600 and 800 hPa, generally higher over land than over ocean. This is so because, over Amazonia, shallow convection causes an increase of bulk entrainment rates at lower levels and because, over West Africa, where entrainment rates are highest, convection is organized in squall lines. These squall lines are associated with strong mesoscale convergence, causing more intense updrafts and stronger turbulence generation in the vicinity of updrafts, increasing the entrainment rates. With the exception of West Africa, entrainment rates differ less across regions than across different environments within the regions. In contrast to what is usually assumed in convective parameterizations, entrainment rates increase with environmental humidity. Furthermore, over ocean, they increase with static stability, while over land, they decrease. In addition, confirming the results of a recent idealized study, entrainment rates increase with convective aggregation, except in regions dominated by squall lines, like over West Africa.
A Generalized Construction of Mutually Orthogonal Complementary Sequence Sets With Non-Power-of-Two Lengths

Recently, mutually orthogonal complementary sequence sets (MOCSSs) have been found many important applications in communication systems, radar, etc. Most of the known constructions of MOCSSs are based on generalized Boolean functions (GBFs) and hence mostly have lengths of power-of-two. A few constructions of MOCSSs are also based on paraunitary (PU) matrices and hence mostly have power-of-an-integer lengths. The objective of this paper is to develop a general framework to construct more MOCSSs consisting of sequences with non-power-of-two lengths. The proposed framework is based on complete complementary codes and even-shift complementary sequence sets.
A New Composite Biomaterial Made from Sunflower Proteins, Urea, and Soluble Polymers Obtained from Industrial and Municipal Biowastes to Perform as Slow Release Fertiliser

Controlled-release fertilizers (CRF) are needed under current agriculture practice to decrease the environmental impact caused by fertilizer doses applied in excess of plants’ uptake rate. Commercial CRF are available. They are manufactured from mineral fertilizers encapsulated into a synthetic polymer matrix or coated by a polymer layer. However, substitution of fossil sourced organic polymers with biopolymers is a major environmental concern. In the present paper, we describe the manufacture by a continuous twin-screw extrusion process, and the mechanical and chemical properties of injection-molded composite pellets containing 90% sunflower protein concentrate (SPC) matrix, and 5–10% of a biopolymer (BP) obtained from municipal biowastes (MBW), and/or urea (U). The reported results show that SPC-BP-U behaves as an efficient eco-friendly CRF. BP contributes to several benefits to the performance of the composite pellets, upon increasing surface hardness, and controlling the formation of ammonia from urea hydrolysis and the release of organic nitrogen. The SPC-BP-U appears a powerful eco-friendly CRF to supply organic C and the three major N, P, and K nutrients to soil and plants. It offers worthwhile scope for being tested in the cultivation of specific plants under the real operational conditions of agriculture practices.
Development of the Learning Instruction Based on Problem Based Learning Models Oriented with Mitigation of Mount Eruption and Lava Floods on the Mathematical Reasoning Ability of Class VIII Students of SMP / MTs

During 2019, the National Disaster Management Agency (BNPB) noted that there had been 3622 disasters in Indonesia, one of which was natural disasters such as volcanic eruptions and cold lava floods. In terms of volcanoes, Indonesia is the most active volcanoes location in the world and it have many tectonic plates that have the potential to cause volcanic eruptions, tsunamis, and earthquakes. Therefore, the Indonesia Government recommends providing education on natural disaster mitigation in schools. The goal of this study was to determine the mathematical reasoning abilities of students and the knowledge and attitudes of students towards mitigating natural disasters. This research is a development research, the development model used in this research is the Plomp model which consists of three phases, namely preliminary research, prototyping phase, and assessment phase. The instruments used during the research were observation sheets, interview guides, questionnaires, validation sheets for learning devices, and tests of students' mathematical reasoning abilities. From this research, it is proven that about 71.4% of MTsN 6 Agam students stated that there has been no learning about natural disaster mitigation. Based on the results of the mathematical reasoning ability test, there was an increase to 72.7% from the initial test result of 25.47%. Natural disaster mitigation knowledge does not have to be provided to students in specific subjects, but can be integrated into existing subjects, one of which is mathematics.