Miracle-dz/UniFAR-FTUs huggingface dataset:
Quantifying international public finance for climate change adaptation in Africa

ABSTRACT Under the United Nations Framework Convention on Climate Change, international financial assistance is expected to support African and other developing countries as they prepare for and adapt to the impacts of climate change. The impact of this finance depends on how much finance is mobilized and where it is targeted. However, there has been no comprehensive quantitative mapping of adaptation-related finance flows to African countries to date. Here we track development finance principally targeting adaptation from bilateral and multilateral funders to Africa between 2014 and 2018. We find that the amounts of finance are well below the scale of investment needed for adaptation in Africa, which is a region with high vulnerability to climate change and low adaptation capacity. Finance targeting mitigation (US$30.6 billion) was almost double that for adaptation (US$16.5 billion). The relative share of each varies greatly among African countries. More adaptation-related finance was provided as loans (57%) than grants (42%) and half the adaptation finance has targeted just two sectors: agriculture; and water supply and sanitation. Disbursement ratios for adaptation in this period are 46%, much lower than for total development finance in Africa (at 96%). These are all problematic patterns for Africa, highlighting that more adaptation finance and targeted efforts are needed to ensure that financial commitments translate into meaningful change on the ground for African communities. Key policy insights Between 2014 and 2018, adaptation-related finance committed by bilateral and multilateral funders to African countries remained well below US$5.5 billion per year, or roughly US$5 per person per year; these amounts are well below the estimates of adaptation costs in Africa. Funders have not strategically targeted support for adaptation activities towards the most vulnerable to climate change African countries. Lessons from countries that have been more successful in accessing finance point to the value of more sophisticated domestic adaptation policies and plans; of alignment with priorities of the NDC; of meeting funding requirements of specific funders; and of the strategic use of climate funds by national planners. A low adaptation finance disbursement ratio in this period in Africa (at 46%) relates to barriers impeding the full implementation of adaptation projects: low grant to loan ratio; requirements for co-financing; rigid rules of climate funds; and inadequate programming capacity within many countries.
Determination of Nutritional Composition of Some Selected Fishes from Hel River of North-East India

Fish is one of the most potential sources of nutrient containing proteins, fats, carbohydrates, vitamins and minerals required for healthy human body maintenance [1]. All over the world, consumption of fish is increasing among the healthconscious populace. Fish is considered as one of the most important sources of high quality animal protein in the diet consisting of essential amino acids for growth and maintenance of human body, and has a crucial role in food security and mitigation of poverty in both rural and urban areas [2]. In comparison to other protein sources like goat and chicken meat, fish is safer, healthier and is also known to be an excellent source of easily digestible animal protein [3]. Fats are important sources of energy, fat-soluble vitamins and unsaturated fatty acids [4]. Recently, there is increasing interest of fish consumption worldwide because of their high content of polyunsaturated fatty acids, especially the ω-3 fatty acids viz. eicosapentaenoic acid (C20:5) and docosahexaenoic acid (C22:6) [5]. These two fatty acids are known as essential fatty acids because they must be obtained from the diet as human Determination of Nutritional Composition of Some Selected Fishes from Hel River of North-East India
Probing β-Cell Biology in Space and Time

β-Cells in the islet of Langerhans have a central role in maintaining energy homeostasis. Understanding the physiology of β-cells and other islet cells requires a deep understanding of their structural and functional organization, their interaction with vessels and nerves, the layout of paracrine interactions, and the relationship between subcellular compartments and protein complexes inside each cell. These elements are not static; they are dynamic and exert their biological actions at different scales of time. Therefore, scientists must be able to investigate (and visualize) short- and long-lived events within the pancreas and β-cells. Current technological advances in microscopy are able to bridge multiple spatiotemporal scales in biology to reveal the complexity and heterogeneity of β-cell biology. Here, I briefly discuss the historical discoveries that leveraged microscopes to establish the basis of β-cell anatomy and structure, the current imaging platforms that allow the study of islet and β-cell biology at multiple scales of resolution, and their challenges and implications. Lastly, I outline how the remarkable longevity of structural elements at different scales in biology, from molecules to cells to multicellular structures, could represent a previously unrecognized organizational pattern in developing and adult β-cells and pancreas biology.
The International Committee of the Red Cross and support for civilian self-protection in Colombia

ABSTRACT How can international humanitarian actors support civilians in their efforts to protect themselves during armed conflicts? This study analyzes the wartime interactions between international organizations and communities with a particular focus on the International Committee of the Red Cross’s (ICRC) community-based protection activities. Although civilians are often viewed as weak and vulnerable noncombatants, the ICRC and other humanitarians can provide communities with crucial backing to implement nonviolent self-protection strategies. I examine these ICRC-community interactions in the municipality of El Bagre in northern Colombia in 2017 during a period of active contestation between ELN insurgents and BACRIM neo-paramilitary groups as the FARC insurgents withdrew from the area. Through participant-observation during an ICRC delegation deployment as well as interviews with civilians and ICRC staff, I find that the ICRC sharing of protection tactics, facilitation of inter-community cooperation, and other technical assistance catalyzed the civilians’ agency and their innovative efforts to avoid, negotiate with, or protest the armed groups. The civilians’ successes and perceptions of security were often amplified because of ICRC involvement. The insights suggest future research agendas and approaches for protecting civilian populations in Colombia and beyond.
The Cholinergic Brain in Parkinson's Disease

The central cholinergic system includes the basal forebrain nuclei, mainly projecting to the cortex, the mesopontine tegmental nuclei, mainly projecting to the thalamus and subcortical structures, and other groups of projecting neurons and interneurons. This system regulates many functions of human behavior such as cognition, locomotion, and sleep. In Parkinson's disease (PD), disruption of central cholinergic transmission has been associated with cognitive decline, gait problems, freezing of gait (FOG), falls, REM sleep behavior disorder (RBD), neuropsychiatric manifestations, and olfactory dysfunction. Neuropathological and neuroimaging evidence suggests that basal forebrain pathology occurs simultaneously with nigrostriatal denervation, whereas pathology in the pontine nuclei may occur before the onset of motor symptoms. These studies have also detailed the clinical implications of cholinergic dysfunction in PD. Degeneration of basal forebrain nuclei and consequential cortical cholinergic denervation are associated with and may predict the subsequent development of cognitive decline and neuropsychiatric symptoms. Gait problems, FOG, and falls are associated with a complex dysfunction of both pontine and basal forebrain nuclei. Olfactory impairment is associated with cholinergic denervation of the limbic archicortex, specifically hippocampus and amygdala. Available evidence suggests that cholinergic dysfunction, alongside failure of the dopaminergic and other neurotransmitters systems, contributes to the generation of a specific set of clinical manifestations. Therefore, a “cholinergic phenotype” can be identified in people presenting with cognitive decline, falls, and RBD. In this review, we will summarize the organization of the central cholinergic system and the clinical correlates of cholinergic dysfunction in PD.
Ageing as “early‐life inertia”: Disentangling life‐history trade‐offs along a lifetime of an individual

The theory that ageing evolves because of competitive resource allocation between the soma and the germline has been challenged by studies showing that somatic maintenance can be improved without impairing reproduction. However, it has been suggested that cost‐free improvement in somatic maintenance is possible only under a narrow range of benign conditions. Here, we show that experimental downregulation of insulin/IGF‐1 signaling (IIS) in C. elegans nematodes, a robustly reproducible life span‐ and health span‐extending treatment, reduces fitness in a complex variable environment when initiated during development but does not reduce fitness when initiated in adulthood. Thus, our results show that the costs and benefits of reduced IIS can be uncoupled when organisms inhabit variable environments, and, therefore, do not provide support for the resource allocation theory. Our findings support the theory that the force of natural selection on gene expression in evolutionarily conserved signaling pathways that shape life‐history traits declines after the onset of reproduction resulting in organismal senescence.
Temporal Changes in Transcripts of MITE Transposable Elements during Rice Endosperm Development

The repression of transcription from transposable elements (TEs) by DNA methylation is necessary to maintain genome integrity and prevent harmful mutations. However, under certain circumstances, TEs are thought to escape from the host defense system and reactivate their transcription. In Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), DNA demethylases target the sequences derived from TEs in the central cell, the progenitor cell for the endosperm in the female gametophyte. This genome-wide DNA demethylation is also observed in the endosperm after fertilization. In this study, we used a custom microarray to survey the transcripts generated from TEs during the rice endosperm development and at selected timepoints in the embryo as a control. The expression patterns of TE transcripts are dynamically up- and downregulated during endosperm development, especially for miniature inverted-repeat transposable elements (MITEs). Surprisingly, some TE transcripts were directionally controlled, while the other DNA transposons and retrotransposons were not. We also discovered the NF-Y binding motif, CCAAT, in the region near the 5′ terminal inverted repeat of Youren, one of the transcribed MITEs in the endosperm. Our results uncover dynamic changes in TE activity during endosperm development in rice.
Towards optimization of oscillatory stimulation during sleep

Background Oscillatory rhythms during sleep such as slow oscillations (SO) and spindles, and most importantly their coupling, are thought to underlie processes of memory consolidation. External slow oscillatory transcranial direct current stimulation (so-tDCS) with a frequency of 0.75 Hz has been shown to improve this coupling and memory consolidation, however, effects varied quite markedly between individuals, studies and species. Objective Here, we aimed to determine how precisely the frequency of stimulation has to match the naturally occurring SO frequency in individuals to optimally improve SO-spindle coupling. Moreover, we systematically tested stimulation durations necessary to induce changes. Methods We addressed these questions by comparing so-tDCS with individually adapted SO frequency to standardized frequency of 0.75Hz in a cross-over design with 28 healthy older participants during napping while systematically varying stimulation train durations between 30s, 2min and 5min. Results Stimulation trains as short as 30s were sufficient to modulate the coupling between SOs and spindle activity. Contrary to our expectations, so-tDCS with standardized frequency indicated stronger aftereffects with regard to SO-spindle coupling in comparison to individualized frequency. Angle and variance of spindle maxima occurrence during the SO cycle were similarly modulated. Conclusion Short stimulation trains were sufficient to induce significant changes in sleep physiology allowing for more trains of stimulation, which provides methodological advantages and possibly even larger effects in future studies. With regard to individualized stimulation frequency, further options of optimization need to be investigated, such as closed-loop stimulation to calibrate stimulation frequency to the SO frequency at time of stimulation onset.
Mutational Signatures as Sensors of Environmental Exposures: Role of Smoking in COVID-19 Vulnerabilities

Background Environmental exposures such as smoking are widely recognized risk factors in the emergence of lung diseases including lung cancer and acute respiratory distress syndrome (ARDS). However, the strength of environmental exposures is difficult to measure, making it challenging to understand their impacts. On the other hand, some COVID-19 patients develop ARDS in an unfavorable disease progression and smoking has been suggested as a potential risk factor among others. Yet initial studies on COVID-19 cases reported contradictory results on the effects of smoking on the disease – some suggest that smoking might have a protective effect against it while other studies report an increased risk. A better understanding of how the exposure to smoking and other environmental factors affect biological processes relevant to SARS-CoV-2 infection and unfavorable disease progression is needed. Approach In this study, we utilize mutational signatures associated with environmental factors as sensors of their exposure level. Many environmental factors including smoking are mutagenic and leave characteristic patterns of mutations, called mutational signatures, in affected genomes. We postulated that analyzing mutational signatures, combined with gene expression, can shed light on the impact of the mutagenic environmental factors to the biological processes. In particular, we utilized mutational signatures from lung adenocarcinoma (LUAD) data set collected in TCGA to investigate the role of environmental factors in COVID-19 vulnerabilities. Integrating mutational signatures with gene expression in normal tissues and using a pathway level analysis, we examined how the exposure to smoking and other mutagenic environmental factors affects the infectivity of the virus and disease progression. Results By delineating changes associated with smoking in pathway-level gene expression and cell type proportions, our study demonstrates that mutational signatures can be utilized to study the impact of exogenous mutagenic factors on them. Consistent with previous findings, our analysis showed that smoking mutational signature (SBS4) is associated with activation of cytokine-mediated signaling pathways, leading to inflammatory responses. Smoking related changes in cell composition were also observed, including the correlation of SBS4 with the expansion of goblet cells. On the other hand, increased basal cells and decreased ciliated cells in proportion were associated with the strength of a different mutational signature (SBS5), which is present abundantly but not exclusively in smokers. In addition, we found that smoking increases the expression levels of genes that are up-regulated in severe COVID-19 cases. Jointly, these results suggest an unfavorable impact of smoking on the disease progression and also provide novel findings on how smoking impacts biological processes in lung.
The activities of LRRK2 and GCase are positively correlated in clinical biospecimens and experimental models of Parkinson’s disease

LRRK2 is a kinase involved in different cellular functions, including autophagy, endolysosomal pathways and vesicle trafficking. Mutations in LRRK2 cause autosomal dominant forms of Parkinson’s disease (PD). Heterozygous mutations in GBA1, the gene encoding the lysosomal enzyme glucocerebrosidase (GCase), are the most common genetic risk factors for PD. Moreover, GCase function is altered in idiopathic PD and in other genetic forms of the disease. Recent work suggests that LRRK2 kinase activity can regulate GCase function. However, both a positive and a negative correlation have been described. To gain insights into the impact of LRRK2 on GCase, we investigated GCase levels and activity in LRRK2 G2019S knockin mice, in clinical biospecimens from PD patients carrying this mutation and in patient-derived cellular models. In these models we found a positive correlation between the activities of LRRK2 and GCase, which was further confirmed in cell lines with genetic and pharmacological manipulation of LRRK2 kinase activity. Overall, our study indicates that LRRK2 kinase activity affects both the levels and the catalytic activity of GCase.
A serotonergic axon-cilium synapse drives nuclear signaling to maintain chromatin accessibility

Chemical synapses between axons and dendrites mediate much of the brain’s intercellular communication. Here we describe a new kind of synapse – the axo-ciliary synapse - between axons and primary cilia. By employing enhanced focused ion beam – scanning electron microscopy on samples with optimally preserved ultrastructure, we discovered synapses between the serotonergic axons arising from the brainstem, and the primary cilia of hippocampal CA1 pyramidal neurons. Functionally, these cilia are enriched in a ciliary-restricted serotonin receptor, 5-hydroxytryptamine receptor 6 (HTR6), whose mutation is associated with learning and memory defects. Using a newly developed cilia-targeted serotonin sensor, we show that optogenetic stimulation of serotonergic axons results in serotonin release onto cilia. Ciliary HTR6 stimulation activates a non-canonical Gαq/11-RhoA pathway. Ablation of this pathway results in nuclear actin and chromatin accessibility changes in CA1 pyramidal neurons. Axo-ciliary synapses serve as a distinct mechanism for neuromodulators to program neuron transcription through privileged access to the nuclear compartment.
Temporal transcriptional response of Candida glabrata during macrophage infection reveals a multifaceted transcriptional regulator CgXbp1 important for macrophage response and drug resistance

Candida glabrata can thrive inside macrophages and tolerate high levels of azole antifungals. These innate abilities render infections by this human pathogen a clinical challenge. How C. glabrata reacts inside macrophages and what is the molecular basis of its drug tolerance are not well understood. Here, we mapped genome-wide RNA polymerase II (RNAPII) occupancy in C. glabrata to delineate its transcriptional responses during macrophage infection in high temporal resolution. RNAPII profiles revealed dynamic C. glabrata responses to macrophage with genes of specialized pathways activated chronologically at different times of infection. We identified an uncharacterized transcription factor (CgXbp1) important for the chronological macrophage response, survival in macrophages, and virulence. Genome-wide mapping of CgXbp1 direct targets further revealed its multi-faceted functions, regulating not only virulence-related genes but also genes associated with drug resistance. Finally, we showed that CgXbp1 indeed also affects azole resistance. Overall, this work presents a powerful approach for examining host-pathogen interaction and uncovers a novel transcription factor important for C. glabrata’s survival in macrophages and drug tolerance.
Agronomic use of produced water in tomato plants (Lycopersicon esculentum L.) under greenhouse conditions

Las estructuras geologicas productoras de hidrocarburos normalmente contienen aguas congenitas y al ser extraidas durante el proceso industrial de produccion de gas o petroleosu composicion es modificada y se le llama “agua producida”. El objetivo del presente estudio fue caracterizar y verificar la factibilidad del uso de aguas producidas provenientes de la zona de exploracion de gas de Sabinas-Piedras Negras de Mexico, para cultivar plantas de tomate bajo condiciones de invernadero. Se establecieron tres tratamientos mezclando aguas producidas provenientes de tres estaciones productoras de gas (Buena Suerte, Monclova 1 y Forasteros), con agua de riego normal. Las proporcionesde las mezclas fueron (mL de aguas producidas por L de agua de riego) 133, 3.4 y 125 respectivamente. Se incluyo un testigo en el que se uso solamente solucion Steiner. Las aguas producidas se analizaron bajo la NOM-143-SEMARNAT-2003, al igual que los tratamientos. Los resultados mostraron que las mezclas con agua producidaproveniente de las estaciones Monclova 1 y Forasteros eran factibles de ser utilizadas para la produccion de tomate, ya que las variables morfologicas evaluadas no presentaron diferencias significativas comparadas con el testigo, aunque las plantas regadas con la mezcla con agua de la estacion Forasteros mostraron disminucion del peso seco de las hojas; pero la concentracion promedio de minerales absorbidos por las plantas fue la que mas se acerco al testigo. El tratamiento con la mezcla de aguas de la estacion Buena Suerte no fue apta para uso agricola porque afecto negativamente el diametro de tallo, el peso seco de la hoja, la longitud de raiz, limito la absorcion mineral, ademas de causar la muerte del 58 % de las plantas.
Factors That Improve Chest Computed Tomography-Defined Sarcopenia Prognosis in Advanced Non-Small Cell Lung Cancer

Background Whether muscle strength and physical performance should be components of sarcopenia remains controversial. This study evaluated the skeletal muscle index derived from computed tomography images at the 12th thoracic vertebra level (T12 SMI), handgrip strength, performance status, and their combination for predicting overall survival in patients with advanced non-small cell lung cancer. Methods Chest computed tomography, handgrip strength measurement, and bioelectrical impedance analysis were performed. Sarcopenia was defined based on the T12 SMI alone or the T12 SMI, handgrip, and/or physical performance (i.e. Asian Working Group for Sarcopenia [AWGS]-defined sarcopenia or severe sarcopenia). Results Overall, 639 participants were included; 488 (76.4%) died. At baseline, 160 (25.0%), 141 (22.1%), and 42 (6.6%) patients had computed tomography-defined sarcopenia, AWGS-defined sarcopenia, and AWGS-defined severe sarcopenia, respectively. Chest computed tomography-defined sarcopenia (hazard ratio [HR], 2.00; 95% confidence interval [CI], 1.65-2.43), AWGS-defined sarcopenia (HR, 2.00; 95% CI, 1.59-2.49), and AWGS-defined severe sarcopenia (HR, 3.01; 95% CI, 2.21-4.09) were more strongly associated with poor prognosis than a performance status score ≥2 (HR, 1.37; 95% CI, 1.10-1.73). Conclusions Adding handgrip strength and the performance status score to chest computed tomography-defined sarcopenia improved its prognostic ability. Oncological sarcopenia research should focus on muscle mass, strength, and function.