Miracle-dz/UniFAR-FTUs huggingface dataset:
Diversity Measurement Indices of Diatom Communities in the Tigris River within Wasit Province, Iraq

The study was conducted to measure diatom species diversity in the lotic ecosystem across the Wasit Province for 12 months. The quantitative study of diatoms (phytoplankton) was investigated in the Tigris river. The density of algae was ranged from 60989 cell×10 3 /l to 112780.82 cell×10 3 /l in the five sites. These algae were belonging to 39 genera. The richness index values ranged from 1.53 at site 5 in January 2016 to 6.34 at site 1 and June2015. Shannon-Weiner diversity index (H ́) was 2.33 in February 2016 and 3.72 in June 2015 both values at site 3, whereas Evenness index was 0.54 at site 5 in March2016 and 0.98 at site 1 in both August2015 and May2016. The lack of homogeneity of the appearance of species indicates the dominance of a few species with high densities, which is an indicator of the existence of environmental pressure. All studied indices showed that the Tigris River quality is suitable for the living aquatic life or may be slightly affected by the pollutants.
Enhanced autophagy and phagocytosis of apoptotic lymphocytes in splenic macrophages of acute ethanol-treated rats: Light and electron microscopic studies.

Autophagy is a prosurvival mechanism for the clearance of damaged cellular components, specifically upon exposure to various stressors. In lymphoid organs, excessive ethanol consumption increases lymphocyte apoptosis, resulting in immunosuppression. However, ethanol-induced autophagy and related phagocytosis of apoptotic lymphocytes in the spleen have not been studied yet. Adult male Wistar rats were injected intraperitoneally either with 5 g/kg ethanol or phosphate-buffered saline (as a control group) and then sacrificed 0, 3, 6, and 24 hours after injection. Light and transmission electron microscopy (TEM) findings indicated enhanced T cell apoptosis in the white pulps of ethanol-treated rats (ETRs) compared with the control group, which peaked at 6 h and was associated with the accumulation of tingible body macrophages (TBMs). These macrophages exhibited an upregulated autophagic response, as evidenced by enhanced LC3-II (a specific marker of autophagosomes) expression, which peaked at 24h. In addition, double labeling immunofluorescence of LC3-II with lysosomal markers revealed the enhanced formation of autolysosomes in TBMs of ETRs, which was associated with suppression of p62 immunostaining, indicating the enhanced autophagic flux. Interestingly, this elevated autophagic response in ETR TBMs was accompanied by evidence of LC3-associated phagocytosis (LAP) of apoptotic splenocytes. This is based on TUNEL/LC3-II double labeling and TEM observations of phagosomes containing apoptotic bodies, enclosed within phagosomal membranes adjacent to the autophagic vacuoles. It can be concluded that enhanced prosurvival autophagy in splenic TBMs of ETRs and clearing of apoptotic lymphocytes via LAP may contribute to preventing secondary necrosis and autoimmune diseases.
Mining oomycete proteomes for phosphatome leads to the identification of specific expanded phosphatases in oomycetes

Abstract Phosphatases are important regulators of protein phosphorylation and various cellular processes, and they serve as counterparts to kinases. In this study, our comprehensive analysis of oomycete complete proteomes unveiled the presence of approximately 3833 phosphatases, with most species estimated to have between 100 and 300 putative phosphatases. Further investigation of these phosphatases revealed a significant increase in protein serine/threonine phosphatases (PSP) within oomycetes. In particular, we extensively studied the metallo‐dependent protein phosphatase (PPM) within the PSP family in the model oomycete Phytophthora sojae. Our results showed notable differences in the expression patterns of PPMs throughout 10 life stages of P. sojae, indicating their vital roles in various stages of oomycete pathogens. Moreover, we identified 29 PPMs in P. sojae, and eight of them possessed accessory domains in addition to phosphate domains. We investigated the biological function of one PPM protein with an extra PH domain (PPM1); this protein exhibited high expression levels in both asexual developmental and infectious stages. Our analysis confirmed that PPM1 is indeed an active protein phosphatase, and its accessory domain does not affect its phosphatase activity. To delve further into its function, we generated knockout mutants of PPM1 and validated its essential roles in mycelial growth, sporangia and oospore production, as well as infectious stages. To the best of our knowledge, this study provides the first comprehensive inventory of phosphatases in oomycetes and identifies an important phosphatase within the expanded serine/threonine phosphatase group in oomycetes.
Inhibition of chromatin condensation disrupts planar cell migration

ABSTRACT Cell migration involves the actin cytoskeleton, and recently recognized nuclear involvement. In this study, we explore the impact of chromatin remodeling on cell migration using NIH 3T3 cells and a scratch wound assay subjected to pharmacological interventions. We inhibit histone deacetylases (HDACs) with Trichostatin A (TSA) and methyltransferase EZH2 with GSK126 to modulate chromatin compaction. Our results indicate that chromatin modifications impair wound closure efficiency, reduce individual cell migration speed, and disrupt migration persistence. Live-cell imaging reveals dynamic intranuclear chromatin remodeling and nuclear shape parameters during migration, influenced by both small- and large-scale chromatin remodeling. The altered nuclear shape is associated with disrupted cell and nuclear mechanics, suggesting a crucial interplay between chromatin remodeling, nuclear mechanics and migration. These findings shed light on the intricate connection between intranuclear chromatin dynamics, nuclear mechanics, and cell migration, providing a basis for further investigations into the molecular mechanisms governing these processes.
Exploring mental health stigma among Indonesian healthcare students towards individuals with mental illnesses: a qualitative study

ABSTRACT Background The social disapproval or stigma surrounding mental illness contributes to the postponement of individuals seeking assistance and frequently undermines therapeutic alliances between mental illness sufferers and health care professionals. Aims This study explored perceptions and attitudes towards individuals with mental illness among college healthcare students in Indonesia. Methods This study used a qualitative method. Twenty five students enrolled in six healthcare programs were interviewed using a semi structured format. The data analysis adopted a thematic analysis. Results Our thematic analysis generated four main themes: (1) general perceptions of mental health and mental illness; (2) knowledge about mental illness; (3) mental health stigma; and (4) mental health stigma campaigns. Conclusions The participants exhibited positive perceptions of mentally ill people. Students understood mental health, and they exhibited positive attitudes toward mentally ill people. Some students have stigma and lack of confidence to assist those who have mental illness. Further efforts are required to acquaint students with mental health issues and facilitate their interaction with mentally ill individuals. Anti-stigma campaigns are required to combat the pervasive stigmatization of individuals with mental illness. It is recommended to conduct a more extensive study about the stigma that students encounter in relation to mentally ill individuals.
FNDC5 genetic polymorphism in patients with peripartum cardiomyopathy.

Background Late in pregnancy or soon after delivery, peripartum cardiomyopathy (PPCM) which is an uncommon type of cardiomyopathy, can develop. To assess the association between the level of irisin expression and (FNDC5) (rs3480) gene polymorphism with peripartum cardiomyopathy. Methods This is a case control study included a thirty female patients with new-onset PPCM and sixty healthy females at the at the peripartum period in same time window for PPCM as a control. For each patient, comprehensive medical history was taken, full clinical assessment was done, ECHO. , FNDC5 (rs3480) & Irisin assay. Results The left ventricle end diastolic dimensions &left atrium diameters were statistically significant higher in patients' group than controls' group (P=0.000 for all), Also left ventricular ejection fraction (%) was statistically significant lower in patients than controls and as regards irisin, its Mean ±SD was lower in patient group than control group (8.44±1.1 vs 10.65±2.31) with (p <0.001) which is considered a significant difference statistically. Conclusion Irisin level was lower in peripartum cardiomyopathic patients when compared with normal individuals and regarding its genotype, the homotype A/A was higher than homotype G/G.
Non-operative Management - The First Option in the Treatment of Blunt Liver and Spleen Trauma in Pediatric Patients.

AIM The aim of the present study is to assess some characteristics of blunt hepatic and splenic injuries in children, the non-operative management (NOM) procedures and efficiency, over a 5-year period in a tertiary hospital for children. Materials and Methods: We conducted a retrospective study on 32 patients with blunt liver and/or spleen injuries. Age, gender, mechanism of injury, hemoglobin and hematocrit levels, lenght of stay and bedrest, imaging diagnosis, hemostatics and transfusions, treatment, and discharge status were evaluated. Results: 58% of patients were males. Mean age was 10.7 years. The main mechanism of injury was motor vehicle accident. Ultrasound (US) and Computed Tomography (CT) found 56.2% patients with spleen injury and 43.8% with liver injuries. On US the most frequent injuries were lacerations, and on CT were splenic-grade III and hepatic-grade II. 84.4% of patients were hospitalized in Intensive Care Unit and 15.6% in the surgical unit. The mean hemoglobin and hematocrit were 10.91g/l and 33%, respectively.The treatment was non-operative for 84.4%, and operative for 15.6%. When discharged, 56.2% of patients were cured and 43.8% were improved. CONCLUSION With a performing multidisciplinary team of surgeons, intensive care therapists and radiologists, NOM in pediatric patients with blunt liver and spleen injuries is safe and effective, may be conducted depending on the hemodynamic stability rather than the lesions' extension, and reduces the ICU lenght of stay, as well as the need for hemostatics and transfusion.
Estimation of anisotropy parameters using intrinsic rock properties in a selected swamp fields in Niger Delta

Total disregard of anisotropy in seismic velocity analysis often accounts for suboptimal imaging especially when prestack depth migration algorithm is used in depth positioning and focusing. The type of anisotropy commonly observed in most sedimentary basins, like the Niger Delta, which comprises of about 70% shale, is the Vertical Transverse Isotropy (VTI), which often affects imaging processes. This type of anisotropy can be accurately quantified by estimating the three Thomsen parameters namely: epsilon (e), delta (δ) and gamma (γ), determination of these parameters will greatly enhances the accurate imaging of events subsurface in prestack depth migration which is used for the proper placement of the events. For this study, the Thomsen parameters, which were derived from well log suites of selected swamp fields of the study area, were used to completely characterize the vertical transverse isotropy (VTI) of the system in our study area, a total of five elastic stiffness moduli were estimated and the values obtained were subsequently used in the estimation of the anisotropy parameters needed. The following values were estimated for the anisotropy parameters from the selected wells; parameter delta (δ) values estimated lies within the ranges -0.16 ≤ δ ≤ 0.13, while for parameter epsilon (e), the values lies within the ranges -0.07 ≤ e ≤ 0.11. The values for parameter gamma (γ) estimated lies within ranges -0.42 ≤ γ ≤ 0.4 while the values for eta (η) lies within the ranges -0.13 ≤ η ≤ 0.27, respectively. The anisotropy parameters values estimated for the selected swamp fields correlate well (0.95%) with the values of the same parameters obtained analytically with a combination of seismic moveout velocity (V NMO ) and vertical velocity from the check-shot data. The anisotropy estimated was found to be higher in shale than in sands, while P-wave anisotropy parameters are observed to be generally smaller than S-wave anisotropy parameters. In the study area, the plots of the anisotropy parameters within the depobelt show a weak anisotropy for the study area.
Early Versus Delayed Laparoscopic Cholecystectomy for Acute Cholecystitis: A Single Center Experience.

Introduction: Acute cholecystitis (AC) represents a public health problem, increasing hospitalization costs, especially determined by the surgical treatment of these patients. Laparoscopic cholecystectomy (LC) has become the therapeutic gold standard, the timing of the intervention: early (ELC) versus late (DLC), is still debated, impacting the results. The primary objective of the study was to compare postoperative outcomes between ELC and DLC. Secondary objectives assessed surgical outcomes from the pre-pandemic period with those from the Covid-19 pandemic. Material and methods: A retrospective observational study is presented of 266 patients diagnosed with AC who were admitted to Clinic I of General Surgery, County Emergency Clinical Hospital of T #226;rgu Mure #351;, from 2018 to 2022. They were classified into the ELC group ( 72 hours from the onset of symptoms) and DLC ( 72 hours from symptom onset) and were further stratified into prepandemic and pandemic cohorts. Data on clinical symptoms, paraclinical data, surgical details, and postoperative course were collected and analyzed. Discussion: The results confirm fewer conversions to open surgery and reduced hospitalization in the ELC group. The pandemic did not significantly alter the timing of surgeries or patient demographics. Conclusion: In conclusion, ELC for AC patients offers significant advantages, justifying its preference over DLC Despite the decrease in the incidence of AC hospitalizations during the pandemic, postoperative outcomes are comparable to those in the pre-pandemic period. Future multicenter studies are recommended for a broader analysis of the efficacy of laparoscopic surgery in emergency settings.
SOTALIA GUIANENSIS POPULATION DENSITY ESTIMATIVE IN THE BAY OF ANTONINA, BRAZIL

The present work aimed to estimate the population density of Sotalia guianensis in the Bay of Antonina, southern Brazilian coast, by linear transects, distance method. The average group size in the total area was 2.57 individuals/group. The study area is 28.1 km2 and it was divided in two sub-areas, an outer area closer to the sea and another more enclosed with a higher fresh water input. In the Sector I, the one with greater marine influence, the density estimation found was D = 3.01 animals/ km2 (Variation Coefficient, VC 24.67%), whereas in Sector II there was no estuarine dolphin record. Also, during the rainy season a higher density value was found in the bay (D = 4.99 individuals/ km2, VC 15.93%). When the area division was ignored an overestimation of density was found (D = 3.80 animals/km2; VC 15.71%) due to the data extrapolation beyond the sampled area, including areas not much used by the dolphins. This superestimation due to the few estuarine dolphins records in the Bay of Antonina and also to the method used, which was biased by existing of sand banks at low tide which made it impossible to access all transects.
Decade-long insights: tracking asbestos-related health impacts among formerly exposed workers in Palermo, Italy.

Background Asbestos is a foremost occupational carcinogen globally. Despite the prohibition under Law 257/1992, Italy persists as one of the European nations most burdened by asbestos-related diseases (ARDs). This research assessed ARD cases in asbestosexposed workers from the Province of Palermo, Italy, spanning 2010-2021. Methods Data acquisition utilized the epidemiological dataset from the 'Service of Prevention and Safety on Work Environment' under the Prevention Department of Palermo's Local Health Authority (LHA). Results Between 2010 and 2021, we identified 245 ARD instances, comprising 163 Asbestosis/Pleural plaques, 41 Lung Cancers, 38 Mesotheliomas, and 3 unspecified cases. Multivariate analysis indicated a notable decline in temporal exposure for mesothelioma (HR=0.933; 95% CI=0.902-0.965) and lung cancer (HR=0.93; 95% CI=0.90-0.978) relative to pleural plaques/asbestosis. Tobacco use displayed a pronounced correlation with lung cancer (smoker HR=64.520 95% CI=13,075-318.390; former smoker HR=20.917 95% CI=4,913-89.048). A significant link was observed between mesothelioma and pleural plaques/asbestosis in those employed in shipbuilding and repair (HR=0.371 95% CI=0.155-0.892). Conclusions ARDs persist in clinical observations, even following the 1992 cessation of asbestos-related activities, emphasizing an enduring public health challenge. Enhancing prevention strategies is paramount, focusing on amplifying anamnestic and occupational data collection, thereby facilitating superior early diagnosis strategies for these maladies in the occupationally exposed cohort.
An integrated sea-land approach for mapping geomorphological and sedimentological features in an urban microtidal wave-dominated beach: a case study from S Sardinia, western Mediterranean

ABSTRACT An integrated cartographic approach has been used to summarize different data (geomorphological, sedimentological, hydrodynamic, ecological and anthropic) from an urban microtidal, wave-dominated beach and adjacent inner shelf in a comprehensive and easily readable mapping output. The study area is located in S Sardinia (Italy, Mediterranean Sea) and focuses on Poetto beach. All the data in this study were processed to produce a Main Map (1:6400 scale) showing the key characteristics of the entire area and three detailed secondary maps (1:56,000 and 1:59,000 scale) that include topographic and eco-graphic profiles, the distribution of sedimentary facies and the main anthropic impact. This map, providing detailed information on the beach dynamics, human impact and the marine ecological status of the Poetto urban beach, represents a useful new tool to facilitate environmental conservation and beach management.
Surveillance of SARS-CoV-2 in wastewater by quantitative PCR and digital PCR: a case study in Shijiazhuang city, Hebei province, China

ABSTRACT In this study, we reported the first long-term monitoring of SARS-CoV-2 in wastewater in Mainland China from November 2021 to October 2023. The city of Shijiazhuang was employed for this case study. We developed a triple reverse transcription droplet digital PCR (RT-ddPCR) method using triple primer-probes for simultaneous detection of the N1 gene, E gene, and Pepper mild mottle virus (PMMoV) to achieve accurate quantification of SARS-CoV-2 RNA in wastewater. Both the RT-ddPCR method and the commercial multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) method were implemented for the detection of SARS-CoV-2 in wastewater in Shijiazhuang City over a 24-month period. Results showed that SARS-CoV-2 was detected for the first time in the wastewater of Shijiazhuang City on 10 November 2022. The peak of COVID-19 cases occurred in the middle of December 2022, when the concentration of SARS-CoV-2 in the wastewater was highest. The trend of virus concentration increases and decreases forming a “long-tailed” shape in the COVID-19 outbreak and recession cycle. The results indicated that both multiplex RT-ddPCR and RT-qPCR are effective in detecting SARS-CoV-2 in wastewater, but RT-ddPCR is capable of detecting low concentrations of SARS-CoV-2 in wastewater which is more efficient. The SARS-CoV-2 abundance in wastewater is correlated to clinical data, outlining the public health utility of this work. Highlights First long-term monitoring of SARS-CoV-2 in wastewater in Mainland China COVID-19 outbreak was tracked in Shijiazhuang City from outbreak to containment Wastewater was monitored simultaneously using RT-ddPCR and RT-qPCR methods Triple primer-probe RT-ddPCR detects N1 and E genes of SARS-CoV-2 and PMMoV
Application of Object Detection Algorithm on SoC-FPGA using OpenCL

Nowadays, object detection algorithms have been widely applied in various aspects of life. However, along with the advancement of these algorithms comes high computational complexity, which requires significant processing time. One approach to reduce processing time is to enhance computational speed by implementing Convolutional Neural Networks (CNNs) on Field-Programmable Gate Arrays (FPGAs). However, using FPGAs presents a challenge, as it requires hardware description languages to design complex CNNs, which can be time-consuming. To overcome this challenge, this paper proposes a novel hardware design approach leveraging the Intel® FPGA SDK (Software Development Kit) for $\mathrm{O}\mathrm{p}\mathrm{e}\mathrm{n}\mathrm{C}\mathrm{L}^{\mathrm{T}\mathrm{M}}$ to implement hardware using the C language with the OpenCL framework. This approach involves implementing a CNN on the DE1-SoC board specifically for object detection tasks.
Personalized Lens Correction Improves Quantitative Fundus Autofluorescence Analysis

Purpose Quantitative fundus autofluorescence (QAF) currently deploys an age-based score to correct for lens opacification. However, in elderly people, lens opacification varies strongly between individuals of similar age, and innate lens autofluorescence is not included in the current correction formula. Our goal was to develop and compare an individualized formula. Methods One hundred thirty participants were examined cross-sectionally, and a subset of 30 participants received additional multimodal imaging 2-week post-cataract-surgery. Imaging included the Scheimpflug principle, anterior chamber optical coherence tomography (AC-OCT), lens quantitative autofluorescence (LQAF), and retinal QAF imaging. Among the subset, least absolute shrinkage and selection operator regression and backward selection was implemented to determine which lens score best predicts the QAF value after lens extraction. Subsequently, a spline mixed model was applied to the whole cohort to quantify the influence of LQAF and Scheimpflug on QAF. Results Age and LQAF measurements were found to be the most relevant variables, whereas AC-OCT measurements and Scheimpflug were eliminated by backward selection. Both an increase in Scheimpflug and LQAF values were associated with a decrease in QAF. The prediction error of the spline model (mean absolute error [MAE] ± standard deviation) of 32.2 ± 23.4 (QAF a.u.) was markedly lower compared to the current age-based formula MAE of 96.1 ± 93.5. Both smooth terms, LQAF (P < 0.01) and Scheimpflug (P < 0.001), were significant for the spline mixed model. Conclusions LQAF imaging proved to be the most predictive for the impact of the natural lens on QAF imaging. The application of lens scores in the clinic could improve the accuracy of QAF imaging interpretation and might allow including aged patients in future QAF studies.