Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of Bread and Durum Wheats from their Diploid Ancestral Species Based on Chloroplast DNA

Abstract Species that have been identified as the genome donors to cultivated polyploid durum and bread wheats (Triticum durum L. and T. aestivum L., respectively) are potential gene sources for the breeding of these two crops. Therefore, their accurate identification facilitates their use in the improvement of these crops. Based on chloroplast DNA analysis (rpL2 and rps16 introns, psbC-trnS, trnT-L, and trnL-F) using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP), an attempt was made in 2018 (Department of Molecular Biology and Biotechnology/AECS) to identify durum and bread wheats from each of their proposed diploid ancestral species (i.e., T. monococcum, T. urartu, Aegilops speltoides, and Ae. tauschii). The use of two PCR markers (psbC-trnS and trnL-F) and three PCR-RFLP locus-enzyme combinations (rps16 intron-Tru 1I, rpL2 intron-Taq I, and trnT-L-Taq I) allowed the identification of all species involved. Reliable and accurate identification of diploid ancestors of durum and bread wheats using these candidate species-specific cpDNA markers will be useful for wheat breeding programs, in situ and ex situ conservation efforts, verification of seed purity in commercial seed stocks, and ensuring identity and integrity of accessions held within a collection does not change through unwanted gene flow or by genetic drift after regeneration by seed.
B-maximal commutators, commutators of B-singular integral operators and B-Riesz potentials on B-Morrey spaces

Abstract In this article, we consider the Laplace-Bessel differential operator Δ B k , n = ∑ i = 1 k ∂ 2 ∂ x i 2 + γ i x i ∂ ∂ x i + ∑ i = k + 1 n ∂ 2 ∂ x i 2 , γ 1 > 0 , … , γ k > 0 . {\Delta }_{{B}_{k,n}}=\mathop{\sum }\limits_{i=1}^{k}\left(\frac{{\partial }^{2}}{\partial {x}_{i}^{2}}+\frac{{\gamma }_{i}}{{x}_{i}}\frac{\partial }{\partial {x}_{i}}\right)+\mathop{\sum }\limits_{i=k+1}^{n}\frac{{\partial }^{2}}{\partial {x}_{i}^{2}},{\gamma }_{1}\gt 0,\ldots ,{\gamma }_{k}\gt 0. Furthermore, we define B-maximal commutators, commutators of B-singular integral operators and B-Riesz potentials associated with the Laplace-Bessel differential operator. Moreover, we also obtain the boundedness of the B-maximal commutator M b , γ {M}_{b,\gamma } and the commutator [ b , A γ ] {[}b,{A}_{\gamma }] of the B-singular integral operator and Hardy-Littlewood-Sobolev-type theorem for the commutator [ b , I α , γ ] {[}b,{I}_{\alpha ,\gamma }] of the B-Riesz potential on B-Morrey spaces L p , λ , γ {L}_{p,\lambda ,\gamma } , when b ∈ BMO γ b\in {\text{BMO}}_{\gamma } .
Genetic diversity and structure of Oriental and European beech populations from Iran and Europe

Abstract Genetic variation is a major component of plant development and adaptation, and recent studies have shown that genetic variation among plant species can have important ecological effects. Oriental beech (Fagus orientalis Lipsky) is a dominant tree species in the Hyrcanian forests, where it occupies approximately 18 % of the forested area. In this study, nine expressed sequence tag simple sequence repeat (EST-SSR) markers were used to determine the genetic diversity and structure of Iranian Oriental beech populations growing at different altitudes. We further compared the genetic structures of the Iranian populations to a F. orientalis and a Fagus sylvatica L. population from the hybrid zone of the two taxa in southeast Europe, and to a F. sylvatica population from central Europe. All populations showed a high genetic diversity, which was similar to other F. orientalis and F. sylvatica populations from Europe. The genetic differentiation among Iranian beech populations was very low. In contrast, distinct genetic differentiation was found between the Iranian populations and the analyzed European populations (F. orientalis and F. sylvatica). One EST-SSR was identified to differentiate (GST: 0.503) between F. orientalis and F. sylvatica with very pronounced allele frequency differences between taxa. Specifically, the Fagus sylvatica characteristic allele 189 was almost absent from all Iranian populations and present in low frequencies in F. orientalis populations from other regions. This study helps to extend the knowledge of genetic diversity and genetic structure of Iranian Oriental beech populations. It further gives insights into the genetic differentiation between F. orientalis populations from the center and the edge of the species’ distribution range as well as between Oriental and European beech.
A phase 3 randomized study evaluating sialic acid extended-release for GNE myopathy

Objective To investigate the efficacy and safety of aceneuramic acid extended-release (Ace-ER), a treatment intended to replace deficient sialic acid, in patients with GNE myopathy. Methods UX001-CL301 was a phase 3, double-blind, placebo-controlled, randomized, international study evaluating the efficacy and safety of Ace-ER in patients with GNE myopathy. Participants who could walk ≥200 meters in a 6-minute walk test at screening were randomized 1:1, and stratified by sex, to receive Ace-ER 6 g/d or placebo for 48 weeks and assessed every 8 weeks. The primary endpoint was change in muscle strength over 48 weeks measured by upper extremity composite (UEC) score. Key secondary endpoints included change in lower extremity composite (LEC) score, knee extensor strength, and GNE myopathy–Functional Activity Scale (GNEM-FAS) mobility domain score. Safety assessments included adverse events (AEs), vital signs, and clinical laboratory results. Results Eighty-nine patients were randomized (Ace-ER n = 45; placebo n = 44). Change from baseline to week 48 for UEC score between treatments did not differ (least square mean [LSM] Ace-ER −2.25 kg vs placebo −2.99 kg; LSM difference confidence interval [CI] 0.74 [−1.61 to 3.09]; p = 0.5387). At week 48, there was no significant difference between treatments for the change in key secondary endpoints: LEC LSM difference (CI) −1.49 (−5.83 to 2.86); knee extension strength −0.40 (−2.38 to 1.58); and GNEM-FAS mobility domain score −0.72 (−2.01 to 0.57). Gastrointestinal events were the most common AEs. Conclusions Ace-ER was not superior to placebo in improving muscle strength and function in patients with GNE myopathy. Classification of evidence This study provides Class I evidence that for patients with GNE myopathy, Ace-ER does not improve muscle strength compared to placebo.
Soluble carbohydrates in legume seeds

Abstract Mature dry legume seeds may contain up to 30 different soluble carbohydrates. Sucrose is a major component of the total soluble carbohydrates; others include the raffinose family oligosaccharides (RFOs; raffinose, stachyose, verbascose) that are mono-, di- and tri-α-galactosyl derivatives of sucrose. Other galactosides may include α-galactosyl derivatives of the cyclitols myo-inositol (galactinol, digalactosyl myo-inositol and trigalactosyl myo-inositol), d-pinitol (galactopinitol A, digalactosyl pinitol A (ciceritol) and trigalactosyl pinitol A; and galactopinitol B; higher galactosyl oligomers of galactopintiol B have rarely been detected), d-chiro-inositol (fagopyritol B1, fagopyritol B2 and fagopyritol B3) and d-ononitol (galactosyl d-ononitol and digalactosyl d-ononitol). Small amounts of myo-inositol, d-pinitol and d-chiro-inositol may also be present. Raffinose, stachyose and verbascose increase late in seed maturation, with 70% of RFOs accumulating after maximum seed dry weight is attained. RFOs are mostly degraded during germination. Sucrose, myo-inositol, d-pinitol and d-chiro-inositol are synthesized in maternal tissues of some legumes and are transported to and unloaded by seed coats into the apoplastic space surrounding developing seed embryos. Free cyclitols may be 60% of total soluble carbohydrates in leaves and 20% in seed coat cup exudates. Increasing the supply of free cyclitols may increase the accumulation of their respective α-galactosides in mature seeds. Seeds with reduced RFO accumulation, but with normal to elevated concentrations of galactosyl cyclitols (including fagopyritols), have normal field emergence and are also tolerant to imbibitional chilling under laboratory conditions. Molecular structures, biosynthetic pathways, accumulation of soluble carbohydrates in response to seed-expressed mutations and the physiological role of galactosides are reviewed.
Palmitoylethanolamide and Cannabidiol Prevent Inflammation-induced Hyperpermeability of the Human Gut In Vitro and In Vivo-A Randomized, Placebo-controlled, Double-blind Controlled Trial.

BACKGROUND AND AIMS We aimed to examine, for the first time, the effect of cannabidiol (CBD) and palmitoylethanolamide (PEA) on the permeability of the human gastrointestinal tract in vitro, ex vivo, and in vivo. METHODS Flux measurements of fluorescein-labeled dextrans 10 (FD10) and fluorescein-labeled dextrans 4 (FD4) dextran across Caco-2 cultures treated for 24 hours with interferon gamma (IFNγ) and tumour necrosis factor alpha (TNFα) (10 ng·mL-1) were measured, with or without the presence of CBD and PEA. Mechanisms were investigated using cannabinoid receptor 1 (CB1), cannabinoid receptor 2 (CB2), transient receptor potential vanilloid 1 (TRPV1), and proliferator activated receptors (PPAR) antagonists and protein kinase A (PKA), nitric oxide synthase, phosphoinositide 3-kinases, extracellular signal-regulated kinases (MEK/ERK), adenylyl cyclase, and protein kinase C (PKC) inhibitors. Human colonic mucosal samples collected from bowel resections were treated as previously stated. The receptors TRPV1, PPARα, PPARδ, PPARγ, CB1, CB2, G-coupled protein receptor 55 (GPR55), G-coupled protein receptor 119 (GPR119), and claudins-1, -2, -3, -4, -5, -7, and -8 mRNA were measured using multiplex. Aquaporin 3 and 4 were measured using enzyme-linked immunosorbent assay (ELISA). A randomized, double-blind, controlled-trial assessed the effect of PEA or CBD on the absorption of lactulose and mannitol in humans taking 600 mg of aspirin. Urinary concentrations of these sugars were measured using liquid chromatography mass spectrometry. RESULTS In vitro, PEA, and CBD decreased the inflammation-induced flux of dextrans (P < 0.0001), sensitive to PPARα and CB1 antagonism, respectively. Both PEA and CBD were prevented by PKA, MEK/ERK, and adenylyl cyclase inhibition (P < 0.001). In human mucosa, inflammation decreased claudin-5 mRNA, which was prevented by CBD (P < 0.05). Palmitoylethanolamide and cannabidiol prevented an inflammation-induced fall in TRPV1 and increase in PPARα transcription (P < 0.0001). In vivo, aspirin caused an increase in the absorption of lactulose and mannitol, which were reduced by PEA or CBD (P < 0.001). CONCLUSION Cannabidiol and palmitoylethanolamide reduce permeability in the human colon. These findings have implications in disorders associated with increased gut permeability, such as inflammatory bowel disease.
Trends and constraints in the production and utilization of cowpea leaves in the arid and semi-arid lands of Kenya

Abstract Cowpea (Vigna unguiculata) leaves are nutritious indigenous vegetables that are produced and consumed among local communities in Kenya. However, seasonal production limits their utilization. The study investigated the changing trends in the consumption and utilization of cowpea leaves among cowpea producing households in arid and semi-arid land (ASAL) areas. A cross-sectional survey of randomly selected households producing and consuming cowpea leaves was carried out in Eastern and Coastal ASALs of Kenya to determine the trends and constraints in the production and utilization of the vegetable, thus evaluating its efficiency as a food security crop. The average household production in a season was found to be 3.03 ± 0.9 of 90 kg bags. Lesser severity of the constraints, poor soils, drought, lack of access to seeds and massive spoilage with an odds ratio of 0.4, 0.9, 2.0 and 2.3, respectively, significantly (p < 0.05) predicted the production quantities among households, R2 = 0.21. The study also found that the reliance on own production among households for sourcing the leaves in-season and off-season was 97.5% and 24.9%, respectively. The households consumed the leaves in boiled (87.5%), sundried (27.5%) or blanched (13.6%) forms. Households in the coastal ASALs significantly (p < 0.05) consumed more of dried forms (odds ratio: 3.3) but less of boiled ones (odds ratio: 0.1) than those in the Eastern parts. Households that had more members or a female deciding the food to be bought had significantly (p < 0.05) higher frequency of consumption of cowpea leaves. Marketing challenges, lack of access to inputs and inadequate postharvest technologies for preservation of the vegetables constrained the production and utilization of cowpea leaves. In order to promote the availability and utilization of cowpea leaves both in and out of season, accessibility of good quality seeds and postharvest management are necessary.
What Drives Youth’s Intention to Migrate Abroad? Evidence from International Survey Data

Abstract Despite the bulk of international migrants being youth, little is known about the factors driving young people’s migration behavior at the global level. Using the individual-level survey data from Gallup World Poll across 139 countries over the period 2010–2016, this study contributes to the literature by exploring a wide range of factors potentially shaping young people’s (aged 15–34) desire, and a more concrete plan, to migrate abroad permanently. Results show that factors, such as holding post-secondary education, being unemployed, and working part-time involuntary, are increasing the desire of youth to migrate abroad as well as the probability that they turn this aspiration into a more concrete plan over the following year. Similarly, having negative expectations about the economic outlook, the number of available job opportunities, and the prospects for upward career mobility are found to increase the propensity to migrate abroad, both among unemployed and employed youth. Results also show that material deprivation may represent a significant push factor behind youth migration, although budgetary constraints may prevent youth from transforming their migration desires into actual plans in low-income countries. Moreover, findings suggest that contextual factors, such as discontent with local amenities and national governments, increase the desire of youth to migrate abroad, but they have little or no influence on the probability that these dreams are turned into more concrete plans. Finally, this study shows that while youth’s and adults’ migration propensities are often driven by the same motives, the influence of education and labor market-related factors on migration intentions is considerably stronger among youth than adults.
Cutting Edge: Biasing Immune Responses by Directing Antigen to Macrophage Fcγ Receptors1

An immune response can deviate toward either a Th1- or Th2-like response. In this work we examine the contribution that activated macrophages and IgG Abs make toward this deviation. The use of activated macrophages as APCs resulted in a strong polarized T cell response that was predominated by IFN-γ. However, when Ag was targeted to FcγRs on these macrophages, the T cell response was reversed and biased toward a Th2-like response. This Th2-like phenotype was stable and was retained when the T cells were subsequently restimulated under nonbiasing conditions. The T cell biasing and its reversal via FcγR was also observed in vivo. Mice vaccinated with IgG-opsonized OVA made high levels of IgG Ab of the IgG1 isotype. These studies demonstrate that the ligation of FcγR on activated macrophages can reverse the Th1 biasing that occurs as a result of innate immune responses to microbial products.
Monetary Policy Transmission on Real Trends in Serbia – VAR Analysis

Abstract The efficiency of channels of monetary transmission varies from country to country and is conditioned by a number of factors that determine the economic and financial system of a country. In order to achieve the set monetary policy objectives, а central bank takes certain measures and employs instruments of monetary policy. Those instruments, however, act indirectly and with a certain lag. Due to these limitations in monetary policy effects, the analysis of the monetary transmission is of essence in every country as it enables its designers to determine an optimum monetary regime. In this paper, an analysis of monetary transmission in the Republic of Serbia is made using the Vector autoregressive model (VAR model). The research conducted is significant due to a current issue of the impact of monetary policy on actual economic trends, both in the developed and developing countries. On the basis of the research it is concluded that, in the time period under observation, the biggest impact on the fluctuations in industrial production in Serbia is that of monetary aggregate, whereas the biggest impact on the fluctuation in prices is that of key policy rate movements. The results of the analysis provide guidelines to monetary authorities to take necessary steps to shorten a lag period and eliminate restrictions in transmitting monetary impulses into real economic values.
Leader as a European Policy for Rural Development in a Multilevel Governance Framework: A Comparison of the Implementation in France, Germany and Italy

Abstract Parts of European rural development policies are meant to empower local decision makers. These policies are implemented in very different multilevel governance contexts in the member states. We question the extent to which the institutional differences at the different levels affect the implementation of LEADER (an approach for Community-led Local Development). This contributes to a better understanding of the causes and consequences of different types of LEADER implementation. The research is based on ten case studies in France, Germany and Italy. First, there is an examination of the three different administration systems and the variations of Rural Development Programmes. Based on analyses of documents and interviews with stakeholders, we analysed institutional differences in the LEADER implementation at local level.
Enumerating the Yield and Purity of PfDNA from Archived, Newly Used mRDTs and Comparison with DBS from a Malaria-Endemic Focus

Abstract Objective: Archived malaria rapid diagnostic test strips (mRDTs) serves as an important source of plasmodium Deoxyribose Nucleic Acid (DNA) in epidemiological studies. The presence of Plasmodium falciparum DNA (PfDNA) in mRDTs (yr. 2016-2017) and newly used ones (yr. 2018) were enumerated with a view to establish the parasite’s optimum genomic DNA volume. Methods: A retrospective study to determine the yield and purity of used mRDTs was carried out on randomly selected mRDTs (2016 – 2018). Both positive and negative mRDTs samples were analyzed with nested Polymerase chain reaction (nPCR). Dried blood spots (DBS) were obtained from study enrolments and analyzed molecularly. nPCR and Agarose gel electrophoresis were used to determine P. falciparum DNA. Results: Agarose gel electrophoresis results showed that only 26 out of the 50 samples eligible for screening were PCR positive for P. falciparum. The following was observed; yrs. : 2016 - 17(34%) with 2.06 X 103 yield, 1.7235 purity; 2017 - 16(32%) with 1.03 X 103 yield, 1.7619 purity and 2018 - 17(34%) with 1.42 X 103 yield, 1.6194 purity. Molecular analysis (P.f. 18Ss rRNA) was determined to ascertain positive result that appeared negative using mRDTs or microscopy. The DNA yield of the DBS for 2018 was 1.66 X 103 and a purity (Optical Density 260/280) of 1.69. The purity was higher than that of the mRDTs with a DNA yield of 1.42 X 103 and 1.62 purity. Conclusion: PfDNA extraction is an important process for malaria PCR screening and the reliability is dependent on pureness and concentration.
A novel age-biomarker-clinical history prognostic index for heart failure with reduced left ventricular ejection fraction

Abstract Purpose A model for predicting the prognosis of patients with heart failure with reduced left ventricular ejection fraction (HFrEF) is currently not available. This study aimed to develop an age-biomarker-clinical history prognostic index (ABC-PI) and validate it for the assessment of individual prognosis. Patients and methods A total of 5,974 HFrEF patients were enrolled and 1,529 were included in this study after excluding missing values and loss to follow-up. Variables that significantly contributed to prediction of all-cause mortality were assessed by Cox regression and latent trait analysis (LTA) was used to validate discrimination of variables. Results After Cox regression, the following seven most significant variables were selected: age, N-terminal pro-B-type natriuretic peptide, renal dysfunction, left ventricular mass index, percutaneous coronary intervention, atrial fibrillation, and New York Heart Association (C-index: 0.801 ± 0.013). After verification by LTA, discrimination of these seven variables was proven. A nomogram was used to form the ABC-PI, and then the total score was set to 100 points. A lower score indicated a higher risk. After verification, the 3-year mortality rate was 34.7% in the high-risk group and only 2.6% in the low-risk group. Conclusion Our novel ABC-PI shows a good performance and does not require re-input in the original model. The ABC-PI can be used to effectively and practically predict the prognosis of HFrEF patients.
Tree-ring reconstruction of June-July mean temperatures in the northern Daxing’an Mountains, China

Abstract In this study, the mean temperature of June to July was reconstructed for the period of 1880 to 2014 by using the Larix gmelinii tree-ring width data for the Mangui region in the northern Daxing’an Mountains, China. The reconstruction accounts for 43.6% of the variance in the temperature observed from AD 1959–2014. During the last 134 years, there were 17 warm years and 17 cold years, which accounted for 12.7% of the total reconstruction years, respectively. Cold episodes occurred throughout 1887–1898 (average value is 14.2°C), while warm episodes occurred during 1994–2014 (15.9°C). Based on this regional study, the warmer events coincided with dry periods and the colder events were consistent with wet conditions. The spatial correlation analyses between the reconstructed series and gridded temperature data revealed that the regional climatic variations were well captured by this study and the reconstruction represented a regional temperature signal for the northern Daxing’an Mountains. In addition, Multi-taper method spectral analysis revealed the existence of significant periodicities in our reconstruction. Significant spectral peaks were found at 29.7, 10.9, 2.5, and 2.2 years. The significant spatial correlations between our temperature reconstruction and the El Niño–Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO) and Solar activity suggested that the temperature in the Daxing’an Mountains area indicated both local-regional climate signals and global-scale climate changes.