Quaternary Ammonium Compound Disinfectant Efficacy Test on The Patients’ Environment in dr. Cipto Mangunkusumo General Hospital

The increasing prevalence of hospital-acquired infection continues to be a global concern until today. The purpose of this study is to determine the efficacies of Caviwipe® and 70% alcohol tissue in the prevention of hospital-acquired infection. This experimental study was conducted on 36 equipment samples in dr. Cipto Mangunkusumo Hospital during April–August 2015. A total of 144 surface samples were collected before and after the disinfection process by both the disinfectants. The sample equipment used and placed in the agar plate for a short period of time; the plates were then incubated. The colony numbers of grown bacteria and fungi were calculated. The data were numerically reported as the microbial colony count. Data were analyzed by using SPSS for Windows. Paired t-test was used for the statistical analysis. The mean colony counts before disinfection by 70% alcohol tissue and Caviwipe® were 11.75 and 17.58, respectively. Meanwhile, the average colony counts after disinfection with 70% alcohol tissue and Caviwipe® were 0.138 and 0.222, respectively. Statistical analysis showed no significant difference between the mean of the colony count before and after disinfection with both disinfectants (p>0.05). Separately, a significant difference of colony count between before and after the disinfection process for each disinfectant was seen (p 0.05) in the efficacies between the Caviwipe® and 70% alcohol tissue in reducing the amount of bacteria and fungi present on the surfaces of the equipment placed close to the patients in a hospital environment. Keywords: disinfectant, environment, hospital, alcohol, Caviwipe®. Uji Efikasi Disinfektan Quaternary Ammonium Compound Disinfectant di Lingkungan Pasien di RSUPN dr. Cipto Mangunkusumo Abstrak Peningkatan prevalensi infeksi yang didapat di rumah sakit masih menjadi perhatian hingga saat ini. Penelitian ini bertujuan membandingkan efektivitas Caviwipe® dan tisu alkohol 70% dalam upaya pencegahan infeksi yang didapat di rumah sakit. Penelitian eksperimental ini dilakukan pada bulan April–August 2015 dengan 36 sampel peralatan di rumah sakit dr. Cipto Mangunkusumo (RSCM). Terdapat 144 titik pengambilan sampel permukaan lingkungan dan peralatan, sebelum dan sesudah disinfeksi dengan kedua disinfektan. Sampel diambil dengan contact agar lalu diinkubasi, kemudian dihitung jumlah koloni bakteri dan jamur yang tumbuh. Data jumlah koloni mikroba dilaporkan sebagai data numerik dan dianalisis dengan perangkat lunak SPSS untuk Windows. Analisis statistik menggunakan paired-t-test. Hitung koloni sebelum disinfeksi dengan tisu alkohol 70% dan Caviwipe® adalah 11,7 dan 17,58. Rerata jumlah koloni sesudah disinfeksi dengan tisu alkohol 70% dan Caviwipe® adalah 0,138 dan 0,222. Analisis statistik menunjukkan tidak ada perbedaan bermakna antara rerata jumlah koloni sebelum dan sesudah disinfeksi dengan kedua jenis disinfektan (p>0,05). Masing-masing disinfektan menunjukkan perbedaan bermakna antara jumlah koloni sebelum dan sesudah disinfeksi (p 0,05). Efikasi antara Caviwipe® dan tisu alkohol 70% dalam menurunkan jumlah bakteri dan jamur di lingkungan rumah sakit yang dekat dengan pasien juga tidak berbeda bermakna. Kata kunci : disinfektan, lingkungan, rumah sakit, alkohol, Caviwipe®