Miracle-dz/UniFAR-FTUs huggingface dataset:
Gender Differences in Uptake, Adherence and Experiences: A Longitudinal, Mixed-Methods Study of a Physical Activity Referral Scheme in Scotland, UK

Physical activity referral schemes (PARS) are implemented internationally to increase physical activity (PA), but evidence of effectiveness for population subgroups is equivocal. We examined gender differences for a Scottish PARS. This mixed-methods, concurrent longitudinal study had equal status quantitative and qualitative components. We conducted 348 telephone interviews across three time points (pre-scheme, 12 and 52 weeks). These included validated self-reported PA and exercise self-efficacy measures and open-ended questions about experiences. We recruited 136 participants, of whom 120 completed 12-week and 92 completed 52-week interviews. PARS uptake was 83.8% (114/136), and 12-week adherence for those who started was 43.0% (49/114). Living in less deprived areas was associated with better uptake (p = 0.021) and 12-week adherence (p = 0.020), and with male uptake (p = 0.024) in gender-stratified analysis. Female adherers significantly increased self-reported PA at 12 weeks (p = 0.005) but not 52 weeks. Males significantly increased exercise self-efficacy between baseline and 52 weeks (p = 0.009). Three qualitative themes and eight subthemes developed; gender perspectives, personal factors (health, social circumstances, transport and attendance benefits) and scheme factors (communication, social/staff support, individualisation and age appropriateness). Both genders valued the PARS. To increase uptake, adherence and PA, PARS should ensure timely, personalised communication, individualised, affordable PA and include mechanisms to re-engage those who disengage temporarily.
Microcanonical and Canonical Ensembles for fMRI Brain Networks in Alzheimer’s Disease

This paper seeks to advance the state-of-the-art in analysing fMRI data to detect onset of Alzheimer’s disease and identify stages in the disease progression. We employ methods of network neuroscience to represent correlation across fMRI data arrays, and introduce novel techniques for network construction and analysis. In network construction, we vary thresholds in establishing BOLD time series correlation between nodes, yielding variations in topological and other network characteristics. For network analysis, we employ methods developed for modelling statistical ensembles of virtual particles in thermal systems. The microcanonical ensemble and the canonical ensemble are analogous to two different fMRI network representations. In the former case, there is zero variance in the number of edges in each network, while in the latter case the set of networks have a variance in the number of edges. Ensemble methods describe the macroscopic properties of a network by considering the underlying microscopic characterisations which are in turn closely related to the degree configuration and network entropy. When applied to fMRI data in populations of Alzheimer’s patients and controls, our methods demonstrated levels of sensitivity adequate for clinical purposes in both identifying brain regions undergoing pathological changes and in revealing the dynamics of such changes.
Long-lived photoinitiated charge separation in carotene-diporphyrin triad molecules

A variety of molecular triads and dyads consisting of covalently linked carotenoid (C) andor porphyrin (P) moieties have been prepared and studied with transient absorption and time-resolved fluorescence techniques. Diporphyrins of the type P A -P B and C-P A -P B triads demonstrate interporphyrin singlet-singlet energy transfer with rate constants ranging from 8.1×10 8 to 2.3×10 10 s −1 . Interporphyrin photoinitiated electron transfer is observed in molecules possessing sufficient thermodynamic driving force to produce P A •+ -P B •− and C-P A •+ -P B •− charge-separated states. The electron-transfer rate constant increases with increasing reaction free energy change for the molecules studied, and rate constants up to 3.5×10 9 s −1 and quantum yields up to 0.68 were measured. The carotenodiporphyrin triad systems undergo a subsequent electron-transfer step to give final C •+ -P A -P B •− states
Laparoscopic vs open complete mesocolic excision with central vascular ligation for right-sided colon cancer

Abstract Complete mesocolic excision (CME) is being performed more frequently and has recently become an established oncologic surgical method for right hemicolectomy. Despite its advantages, such as its association with early mobilization, a short hospital stay, early bowel movement, mild postoperative pain, and good cosmesis, CME is technically demanding and carries the risk of severe complications. This study aims to compare the clinical, pathological, and oncological results of open and laparoscopic right hemicolectomy with CME. The data of 76 patients who underwent right hemicolectomy with CME and high vascular ligation were reviewed retrospectively. The patients were divided into 2 groups according to whether the open or laparoscopic technique was used. Thirty-two patients underwent open right hemicolectomy, and 44 patients underwent laparoscopic right hemicolectomy. The 2 groups were similar in age, sex, American Society of Anesthesiologists class, abdominal surgical history, tumor localization, and operation time. No significant differences were found regarding the specimen length, tumor size, harvested lymph nodes, number of metastatic lymph nodes, or tumor grade. According to the Clavien–Dindo classification system, the laparoscopic group had significantly fewer complications than did the open group (11.4% vs 31.2%; P = .04). The open group had a longer postoperative hospital stay than did the laparoscopic hemicolectomy group (9.9 ± 4.7 vs 7.2 ± 3.1 days; P = .002). In addition, the groups were similar with respect to disease-free survival (P = .14) and overall survival (P = .06). The data in this study demonstrated that no differences exist between the open and laparoscopic techniques concerning pathological and oncological results. However, significantly fewer complications and a shorter length of hospital stay were observed in the laparoscopic group than in the open group. Laparoscopic right hemicolectomy with CME and central vascular ligation is a safe and feasible surgical procedure and should be considered the standard technique for right-sided colon cancer.
Quaternary diversification of a columnar cactus in the driest place on earth.

PREMISE The cactus family (Cactaceae) is a speciose lineage with an almost entirely New World distribution. The genus Eulychnia with eight currently recognized species is endemic to the Atacama and Peruvian Deserts. Here we investigated the phylogeny of this group based on a complete taxon sampling to elucidate species delimitation and biogeographic history of the genus. METHODS A family-wide Bayesian molecular clock dating based on plastid sequence data was conducted to estimate the age of Eulychnia and its divergence from its sister genus Austrocactus. A second data set obtained from genotyping by sequencing (GBS) was analyzed, using the family-wide age estimate as a secondary calibration to date the GBS phylogeny using a penalized likelihood approach. Ancestral ranges were inferred employing the dispersal extinction cladogenesis approach. RESULTS Our GBS phylogeny of Eulychnia was fully resolved with high support values nearly throughout the phylogeny. The split from Austrocactus occurred in the late Miocene, and Eulychnia diversified during the early Quaternary. Three lineages were retrieved: Eulychnia ritteri from Peru is sister to all Chilean species, which in turn fall into two sister clades of three and four species, respectively. Diversification in the Chilean clades started in the early Pleistocene. Eulychnia likely originated at the coastal range of its distribution and colonized inland locations several times. CONCLUSIONS Diversification of Eulychnia during the Pleistocene coincides with long periods of hyperaridity alternated with pluvial phases. Hyperaridity caused habitat fragmentation, ultimately leading to speciation and resulting in the current allopatric distribution of taxa.
Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis

ABSTRACT This study investigated dexamethasone-treatment, shedding routes, tissue antigen distribution, and pathology of caprine Brucellosis. Eighteen non-pregnant goats were randomly grouped into A, B, and C. Group A was administered dexamethasone for 7 days at 2 mg/kg before inoculating 0.5 mL B. melitensis at 107 CFU ocularly while group B was inoculated 0.5 mL B. melitensis only, and C as control negative. Blood samples, ocular, nasal, and vaginal swabs were obtained for evaluation. Three goats were sacrificed from each group at days 21 and 42 post-inoculation (pi) and selected tissues collected for PCR, histopathology, and immunohistochemistry. Brucella melitensis was detected in the ocular swabs of group A significantly higher than group B. Shedding was prolonged in group A compared to B. The overall shedding was 22.2% in group A and 9.4% in group B. The uterus of both groups A and B revealed mild inflammation and microgranuloma, extensive necrotic lesions in lymph nodes. Liver showed multifocal necrosis predominantly in group A. Lesion scoring showed significantly higher scores in A compared to B. Strong immunostaining was observed in the liver, lungs, and spleen, predominantly at day 21 pi. This study demonstrated dexamethasone prolonged shedding, tissue antigen distribution, and pathology in dexamethasone-treated goats.
Cytotoxic Alkaloids Derived from Marine Sponges: A Comprehensive Review

Marine sponges (porifera) have proved to be a prolific source of unique bioactive secondary metabolites, among which the alkaloids occupy a special place in terms of unprecedented structures and outstanding biological activities. Identification of active cytotoxic alkaloids extracted from marine animals, particularly sponges, is an important strive, due to lack of knowledge on traditional experiential and ethnopharmacology investigations. In this report, a comprehensive survey of demospongian bioactive alkaloids in the range 1987–2020 had been performed with a special emphasis on the potent cytotoxic activity. Different resources and databases had been investigated, including Scifinder (database for the chemical literature) CAS (Chemical Abstract Service) search, web of science, Marin Lit (marine natural products research) database. More than 230 representatives of different classes of alkaloids had been reviewed and classified, different genera belonging to the phylum porifera had been shown to be a prolific source of alkaloidal molecules, including Agelas sp. , Suberea sp. , Mycale sp. , Haliclona sp. , Epipolasis sp. , Monanchora sp. , Crambe sp. , Reniera sp. , and Xestospongia sp. , among others. The sufficient production of alkaloids derived from sponges is a prosperous approach that requires more attention in future studies to consider the constraints regarding the supply of drugs, attained from marine organisms.
Influência de calcário e fósforo no desenvolvimento e produção de variedades de maracujazeiro-amarelo

Com objetivo de avaliar o efeito da aplicacao de calcario e fosforo sobre o desenvolvimento de duas variedades de maracujazeiro, foi desenvolvido experimento em casa de vegetacao no Instituto de Ciencias Agrarias da Universidade Federal Rural da Amazonia (UFRA). Utilizou-se como substrato Latossolo Amarelo distrofico, textura media. O delineamento experimental foi em blocos casualizados, em esquema fatorial 4x4x2, com 32 tratamentos e 4 repeticoes, totalizando 128 parcelas. Os fatores estudados foram quatro doses de fosforo (0; 100; 200 e 300 mg dm-3 de P) na forma de superfosfato triplo (SFT); quatro niveis de saturacao por bases (inicial = 15%, 40%, 65% e 90%) e duas variedades de maracujazeiro (Golden Star e CPATU-Casca fina). Como corretivo de acidez do solo, foram utilizados carbonato de calcio (CaCO3) e carbonato de magnesio (MgCO3). Apos 50 dias da instalacao do experimento, realizaram-se as avaliacoes nas variaveis biologicas indicativas do desenvolvimento da planta, como: altura, diâmetro do caule e massa seca da parte aerea. A aplicacao combinada de fosforo e de calcario influenciou positivamente no desenvolvimento e na massa seca de plantas de maracujazeiro. A maior producao de massa seca foi obtida com aplicacao combinada de 160 mg dm-3 de P em solo com saturacao por bases estimada de 47%, que esteve associada as concentracoes de P e de Ca de 143 mg dm-3 e 2,9 cmolc dm-3 no solo; e a teores de 2,6 e 10,8 g kg-1 na massa seca da parte aerea, respectivamente. A variedade CPATU Casca fina foi superior, em termos de diâmetros medios de caule e massa seca da parte aerea.
Separation Anxiety Disorder in Panic Disorder Patients with and without Comorbid Agoraphobia

Objective: Previous studies have focused on the relationship between childhood separation anxiety disorder (SAD) and adult panic disorder (PD)-agoraphobia. It is not clear enough whether SAD, which continues into adulthood, is associated with PD with and without comorbid agoraphobia in adult patients. Our primary hypothesis was that PD patients with comorbid agoraphobia had a higher rate of SAD that continues into adulthood than those without agoraphobia. We also hypothesized that adulthood SAD symptoms were more likely to be associated with PD-agoraphobia than PD without agoraphobia. Method: 151 patients who were diagnosed with PD with (n = 106), and without comorbid agoraphobia (n = 45) were compared using Panic and Agoraphobia Scale, Structured Clinical Interview for Separation Anxiety Symptoms, Separation Anxiety Symptom Inventory, Adult Separation Anxiety Questionnaire, and State-Trait Anxiety Inventory. We performed Student’s t-test, Pearson correlation test, and multiple linear regression analysis in this study. Results: PD patients with comorbid agoraphobia were more likely to have SAD both in childhood and adulthood (p = .028), than those without agoraphobia. There were mild to moderate significant correlations between PD severity and state anxiety (p = .002), trait anxiety (p = .006), and SAD in childhood (p = .049), and in adulthood (p = .001). SAD in adulthood (β = 0.278, Exp(B) = 0.136, p = . 003), and state anxiety (β = 0.236, Exp(B) = 0.164, p = . 012) significantly predicted the severity of PD in patients with comorbid agoraphobia. Conclusion: SAD that continues in adulthood may be related to the severity of PD in patients with agoraphobia. Our findings might provide some evidence of the role of SAD in adulthood in patients with adult-onset PD-agoraphobia.
Demystifying Chronic Kidney Disease of Unknown Etiology (CKDu): Computational Interaction Analysis of Pesticides and Metabolites with Vital Renal Enzymes

Chronic kidney disease of unknown etiology (CKDu) has been recognized as a global non-communicable health issue. There are many proposed risk factors for CKDu and the exact reason is yet to be discovered. Understanding the inhibition or manipulation of vital renal enzymes by pesticides can play a key role in understanding the link between CKDu and pesticides. Even though it is very important to take metabolites into account when investigating the relationship between CKDu and pesticides, there is a lack of insight regarding the effects of pesticide metabolites towards CKDu. In this study, a computational approach was used to study the effects of pesticide metabolites on CKDu. Further, interactions of selected pesticides and their metabolites with renal enzymes were studied using molecular docking and molecular dynamics simulation studies. It was evident that some pesticides and metabolites have affinity to bind at the active site or at regulatory sites of considered renal enzymes. Another important discovery was the potential of some metabolites to have higher binding interactions with considered renal enzymes compared to the parent pesticides. These findings raise the question of whether pesticide metabolites may be a main risk factor towards CKDu.
Metal Content of Plants and Soils in a Tree Nursery Treated with Composted Sludge

Digested sewage sludge compost from Washington, DC, was applied to a Downer sand at rates of 0, 56, 112, 224, and 448 dry metric tons/ha. A fertilized control treatment was also utilized. Pearl millet (Pennisetum americanum (L.) K. Schum. ) was grown as a summer cover crop prior to fall tree seeding. Leaf samples of red oak (Quercus ruba L.) and black walnut (Juglans nigra L.) seedlings were taken the summer of the first growing season. The total metal contents (.mu.g/g) of the compost were 2154 Zn, 517 Cu, 13.9 Cd, and 69 Ni. The treated soils were monitored for DTPA extractable Zn, Cu, Cd, and Ni throughout the 2.4 years of the experiment. No visual, foliar effects were noted on the growth of pearl millet or tree seedlings from the increasing rates of compost, however, pearl millet tissue Cd levels were significantly increased with increasing levels of compost from about 0.40 to about 1.62 . mu.g/g. Metal contents of the tree seedlings were low and within normal levels recorded in the literature. Extractable soil Zn, Cd, and Cu decreased with time, however, soil Ni extractability tended to increase with time. Compost additions resulted in an increased soil pH which was maintainedmore » throughout the experiment. Poor correlations (r/sup 2/ ranged from 0.002 to 0.543) were obtained between DTPA extractable metals and metal concentrations of pearl millet, red oak, or black walnut. « less
Rates and prospective psychosocial correlates of pre-loss grief in cancer and dementia family members

This study examined changes in Pre-Loss Grief (PLG) among individuals whose family member has a life limiting illness and how baseline psychosocial factors predicted PLG. This two-wave study recruited family members of advanced cancer (N = 100) and dementia (N = 38) patients. A subsample completed 1-month follow-up (Cancer = 33; Dementia = 28). Higher caregiver burden and female participants predicted greater PLG at follow-up, accounting for baseline PLG. Family members of dementia patients (10.5%) were significantly more likely than family members of cancer patients (2.1%) to be in the “severe PLG” group. Findings demonstrate psychosocial factors related to PLG, informing interventions aimed at reducing PLG.
Paracellular and Transcellular Leukocytes Diapedesis Are Divergent but Interconnected Evolutionary Events

Infiltration of the endothelial layer of the blood-brain barrier by leukocytes plays a critical role in health and disease. When passing through the endothelial layer during the diapedesis process lymphocytes can either follow a paracellular route or a transcellular one. There is a debate whether these two processes constitute one mechanism, or they form two evolutionary distinct migration pathways. We used artificial intelligence, phylogenetic analysis, HH search, ancestor sequence reconstruction to investigate further this intriguing question. We found that the two systems share several ancient components, such as RhoA protein that plays a critical role in controlling actin movement in both mechanisms. However, some of the key components differ between these two transmigration processes. CAV1 genes emerged during Trichoplax adhaerens, and it was only reported in transcellular process. Paracellular process is dependent on PECAM1. PECAM1 emerged from FASL5 during Zebrafish divergence. Lastly, both systems employ late divergent genes such as ICAM1 and VECAM1. Taken together, our results suggest that these two systems constitute two different mechanical sensing mechanisms of immune cell infiltrations of the brain, yet these two systems are connected. We postulate that the mechanical properties of the cellular polarity is the main driving force determining the migration pathway. Our analysis indicates that both systems coevolved with immune cells, evolving to a higher level of complexity in association with the evolution of the immune system.
Improvement of Arterial Stiffness One Month after Bariatric Surgery and Potential Mechanisms

Arterial stiffness (AS) is an independent predictor of cardiovascular risk. We aimed to analyze changes (Δ) in AS 1-month post-bariatric surgery (BS) and search for possible pathophysiological mechanisms. Patients with severe obesity (43% hypertensives) were prospectively evaluated before and 1-month post-BS, with AS assessed by pulse-wave velocity (PWV), augmentation index (AIx@75) and pulse pressure (PP). Ambulatory 24 h blood pressure (BP), anthropometric data, renin-angiotensin-aldosterone system (RAAS) components and several adipokines and inflammatory markers were also analyzed. Overall reduction in body weight was mean (interquartile range (IQR)) = 11.0% (9.6–13.1). A decrease in PWV, AIx@75 and PP was observed 1-month post-BS (all, p < 0.01). There were also significant Δ in BP, RAAS components, adipokines and inflammatory biomarkers. Multiple linear regression adjusted models showed that Δaldosterone was an independent variable (B coeff.95%CI) for final PWV (B = −0.003, −0.005 to 0.000; p = 0.022). Angiotensin-converting enzyme (ACE)/ACE2 and ACE were independent variables for final AIx@75 (B = 0.036, 0.005 to 0.066; p = 0.024) and PP (B = 0.010, 0.003 to 0.017; p = 0.01), respectively. There was no correlation between ΔAS and anthropometric changes nor with Δ of adipokines or inflammatory markers except high-sensitivity C-reactive protein (hs-CRP). Patients with PWV below median decreased PWV (mean, 95%CI = −0.18, −0.25 to −0.10; p < 0.001) and both AIx@75 and PP at 1-month, but not those with PWV above median. In conclusion, there is an improvement in AS 1-month post-BS that correlates with ΔBP and Δrenin-angiotensin-aldosterone components. The benefit is reduced in those with higher PWV.
Phylogenetic Curved Optimal Regression for Adaptive Trait Evolution

Regression analysis using line equations has been broadly applied in studying the evolutionary relationship between the response trait and its covariates. However, the characteristics among closely related species in nature present abundant diversities where the nonlinear relationship between traits have been frequently observed. By treating the evolution of quantitative traits along a phylogenetic tree as a set of continuous stochastic variables, statistical models for describing the dynamics of the optimum of the response trait and its covariates are built herein. Analytical representations for the response trait variables, as well as their optima among a group of related species, are derived. Due to the models’ lack of tractable likelihood, a procedure that implements the Approximate Bayesian Computation (ABC) technique is applied for statistical inference. Simulation results show that the new models perform well where the posterior means of the parameters are close to the true parameters. Empirical analysis supports the new models when analyzing the trait relationship among kangaroo species.