Miracle-dz/UniFAR-FTUs huggingface dataset:
An Exploration of Motivation for Disaster Engagement and Its Related Factors among Undergraduate Nursing Students in Taiwan

The purpose of the study was to explore the levels of and relationships between disaster nursing competence, anticipatory disaster stress, and the motivation for disaster engagement among undergraduate nursing students in Taiwan. A cross-sectional research design was applied. Using convenience sampling, 90 nursing students participated with an 86.54% response rate. The Disaster Core Competencies Questionnaire, Anticipatory Disaster Stress Questionnaire, and Motivation for Disaster Engagement Questionnaire were used to collect data. The Pearson correlation and ANOVA were used to analyze the data. Results showed that students’ level of disaster nursing competence was low, anticipatory disaster stress was not high, and motivation for disaster engagement was high. Motivation for disaster engagement was positively correlated with anticipatory disaster stress. Students who were more willing to participate in disaster management had a higher level of anticipatory disaster stress and motivation for disaster engagement. It is suggested that healthcare institutions and schools should work together to design disaster education plans using innovative teaching/learning strategies to increase students’ willingness and motivation for disaster engagement.
Clofazimine enhances the efficacy of BCG revaccination via stem cell-like memory T cells

Tuberculosis (TB) is one of the deadliest diseases, claiming ~2 million deaths annually worldwide. The majority of people in TB endemic regions are vaccinated with Bacillus Calmette Guerin (BCG), which is the only usable vaccine available. BCG is efficacious against meningeal and disseminated TB in children, but protective responses are relatively short-lived and fail to protect against adult pulmonary TB. The longevity of vaccine efficacy critically depends on the magnitude of long-lasting central memory T (TCM) cells, a major source of which is stem cell-like memory T (TSM) cells. These TSM cells exhibit enhanced self-renewal capacity as well as to rapidly respond to antigen and generate protective poly-functional T cells producing IFN-γ, TNF-α, IL-2 and IL-17. It is now evident that T helper Th 1 and Th17 cells are essential for host protection against TB. Recent reports have indicated that Th17 cells preserve the molecular signature for TSM cells, which eventually differentiate into IFN-γ-producing effector cells. BCG is ineffective in inducing Th17 cell responses, which might explain its inadequate vaccine efficacy. Here, we show that revaccination with BCG along with clofazimine treatment promotes TSM differentiation, which continuously restores TCM and T effector memory (TEM) cells and drastically increases vaccine efficacy in BCG-primed animals. Analyses of these TSM cells revealed that they are predominantly precursors to host protective Th1 and Th17 cells. Taken together, these findings revealed that clofazimine treatment at the time of BCG revaccination provides superior host protection against TB by increasing long-lasting TSM cells.
Genome-wide transcriptomic analysis of microglia reveals impaired responses in aged mice after cerebral ischemia

Senescence-associated alterations in microglia may have profound impact on cerebral homeostasis and stroke outcomes. However, the lack of a transcriptome-wide comparison between young and aged microglia in the context of ischemia limits our understanding of aging-related mechanisms. Herein, we performed RNA sequencing analysis of microglia purified from cerebral hemispheres of young adult (10-week-old) and aged (18-month-old) mice five days after distal middle cerebral artery occlusion or after sham operation. Considerable transcriptional differences were observed between young and aged microglia in healthy brains, indicating heightened chronic inflammation in aged microglia. Following stroke, the overall transcriptional activation was more robust (>13-fold in the number of genes upregulated) in young microglia than in aged microglia. Gene clusters with functional implications in immune inflammatory responses, immune cell chemotaxis, tissue remodeling, and cell-cell interactions were markedly activated in microglia of young but not aged stroke mice. Consistent with the genomic profiling predictions, post-stroke cerebral infiltration of peripheral immune cells was markedly decreased in aged mice compared to young mice. Moreover, post-ischemic aged microglia demonstrated reduced interaction with neighboring neurons and diminished polarity toward the infarct lesion. These alterations in microglial gene response and behavior may contribute to aging-driven vulnerability and poorer recovery after ischemic stroke.
Changes in leaf nitrogen and carbohydrates underlie temperature and CO2 acclimation of dark respiration in five boreal tree species

We tested the hypothesis that acclimation of foliar dark respiration to CO 2 concentration and temperature is associated with adjustments in leaf structure and chemistry. Populus tremuloides Michx. , Betula papyrifera Marsh. , Larix laricina (Du Roi) K. Koch, Pinus banksiana Lamb. , and Picea mariana (Mill.) B.S.P. were grown from seed in combined CO 2 (370 or 580 μmol mol -1 ) and temperature treatments (18/12, 24/18, or 30/24 °C). Temperature and CO 2 effects were predominately independent. Specific respiration rates partially acclimated to warmer thermal environments through downward adjustment in the intercept, but not Q 10 of the temperature-response functions. Temperature acclimation of respiration was larger for conifers than broad-leaved species and was associated with pronounced reductions in leaf nitrogen concentrations in conifers at higher growth temperatures. Short-term increases in CO 2 concentration did not inhibit respiration. Growth in the elevated CO 2 concentration reduced leaf nitrogen and increased non-structural carbohydrate concentrations. However, for a given nitrogen concentration, respiration was higher in leaves grown in the elevated CO 2 concentration, as rates increased with increasing carbohydrates. Across species and treatments, respiration rates were a function of both leaf nitrogen and carbohydrate concentrations (R 2 = 0.71, P < 0.0001). Long-term acclimation of foliar dark respiration to temperature and CO 2 concentration is largely associated with changes in nitrogen and carbohydrate concentrations.
Urinary Tract Infection in Febrile Children with Sickle Cell Disease Who Present to the Emergency Room with Fever

Sickle cell disease (SCD) patients are thought to be at higher risk for urinary tract infections (UTIs) compared to the general population secondary to increased sickling, abnormal urinary acidification, and an inability to concentrate the urine. The incidence of UTI in febrile children with SCD in the United States is unknown. Our objectives were to determine the rate of UTI among febrile SCD children and describe the risk factors for UTI in this population. We conducted a retrospective chart review of all febrile SCD patients <4 years of age who presented to a pediatric emergency department from 2012–2017 and who had a sterile sample of urine for analysis. A total of 167 febrile patients with SCD with 464 visits were identified. The majority were African American (95.2%), female (58.7%), and had hemoglobin SS (HbSS) (65.3%). The rate of UTI was 4.1%. All patients with a UTI were African American females with a median age of 19 months (IQR 12–30). On regression analysis, no risk factors were associated with a UTI. We found the rate of UTI in febrile young children with SCD was comparable to non-SCD children. Larger studies are required to identify the presence of risk factors for UTI in this population.
Cardiac Energetics in Patients With Aortic Stenosis and Preserved Versus Reduced Ejection Fraction

Supplemental Digital Content is available in the text. Background: Why some but not all patients with severe aortic stenosis (SevAS) develop otherwise unexplained reduced systolic function is unclear. We investigate the hypothesis that reduced creatine kinase (CK) capacity and flux is associated with this transition. Methods: We recruited 102 participants to 5 groups: moderate aortic stenosis (ModAS) (n=13), SevAS, left ventricular (LV) ejection fraction ≥55% (SevAS-preserved ejection fraction, n=37), SevAS, LV ejection fraction <55% (SevAS-reduced ejection fraction, n=15), healthy volunteers with nonhypertrophied hearts with normal systolic function (normal healthy volunteer, n=30), and patients with nonhypertrophied, non–pressure-loaded hearts with normal systolic function undergoing cardiac surgery and donating LV biopsy (non–pressure-loaded heart biopsy, n=7). All underwent cardiac magnetic resonance imaging and 31P magnetic resonance spectroscopy for myocardial energetics. LV biopsies (AS and non–pressure-loaded heart biopsy) were analyzed for CK total activity, CK isoforms, citrate synthase activity, and total creatine. Mitochondria-sarcomere diffusion distances were calculated by using serial block-face scanning electron microscopy. Results: In the absence of failure, CK flux was lower in the presence of AS (by 32%, P=0.04), driven primarily by reduction in phosphocreatine/ATP (by 17%, P<0.001), with CK kf unchanged (P=0.46). Although lowest in the SevAS-reduced ejection fraction group, CK flux was not different from the SevAS-preserved ejection fraction group (P>0.99). Accompanying the fall in CK flux, total CK and citrate synthase activities and the absolute activities of mitochondrial-type CK and CK-MM isoforms were also lower (P<0.02, all analyses). Median mitochondria-sarcomere diffusion distances correlated well with CK total activity (r=0.86, P=0.003). Conclusions: Total CK capacity is reduced in SevAS, with median values lowest in those with systolic failure, consistent with reduced energy supply reserve. Despite this, in vivo magnetic resonance spectroscopy measures of resting CK flux suggest that ATP delivery is reduced earlier, at the moderate AS stage, where LV function remains preserved. These findings show that significant energetic impairment is already established in moderate AS and suggest that a fall in CK flux is not by itself a necessary cause of transition to systolic failure. However, because ATP demands increase with AS severity, this could increase susceptibility to systolic failure. As such, targeting CK capacity and flux may be a therapeutic strategy to prevent and treat systolic failure in AS.
Management of Osteoarthritis During the COVID‐19 Pandemic

The pandemic spread of the new coronavirus disease 2019 (COVID‐19) infection in China first, and all over the world at present, has become a global health emergency due to the rapidly increasing number of affected patients. Currently, a clear relationship between COVID‐19 infection incidence and/or complications due to chronic or occasional treatments for other pathologies is still not clear, albeit the COVID‐19 pandemic may condition the treatment strategy of complex disorders, such as osteoarthritis (OA). Importantly, OA is the most common age‐related joint disease, affecting more than 80% of people older than the age of 55, an age burden also shared with the highest severity in COVID‐19 patients. OA patients often show a large array of concomitant pathologies, such as diabetes, inflammation, and cardiovascular diseases that are again shared with COVID‐19 patients and may therefore increase complications. Moreover, different OA treatments, such as NSAIDs, paracetamol, corticosteroids, opioids, or other molecules have a wide array of iatrogenic effects, potentially increasing COVID‐19 secondary infection incidence or complications. In this review we critically analyze the evidence on either negative or positive effects of drugs commonly used to manage OA in this particular scenario. This would provide orthopedic surgeons in particular, and physicians, pharmacologists, and clinicians in general, a comprehensive description about the safety of the current pharmacological approaches and a decision‐making tool to treat their OA patients as the coronavirus pandemic continues.
Corneal donation for research versus for transplantation: A-year prospective study of acceptance rates in a French University Hospital

Fresh corneal donation is essential for basic and preclinical research, but more unknown to public and the medical teams than donation for transplantation: it may raise concerns. We prospectively compared the acceptance rates and the characteristics of targeted corneal donation for research versus donation for transplantation during one year. The Agence de la Biomédecine authorized us to procure fresh corneas targeted for research, only from the donors with medical contraindications for transplantation, in order not to increase grafts shortage. Three nurses from the hospital coordination team of Saint-Etienne University Hospital, obtained consent for research and transplantation in parallel, screening all intra-hospital deaths cases, following standard protocol to check no refusal from families, despite the French opt-out system. They contacted 127 families for research and 244 for transplantation, in 71% of cases by telephone. Consent was obtained in 62% of cases for research and 54% for transplantation (P = 0.135). The main contraindication for transplantation was the cognitive disorders (66%) followed by the blood cancers (8%). This new specific activity, providing new source of fresh corneas for research immediately usable without any eyebank storage steps, didn’t reduce the number of corneas procured for transplantation versus previous years (P = 0.998). Donors in the research group were 10 years older (P<0.001) without difference regarding endothelial cell quality (P = 0.071), allowing maximal clinical relevance for protocols using these fresh human scientific corneas provided by targeted donation.
COVID-19: The Inflammation Link and the Role of Nutrition in Potential Mitigation

The novel coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has engulfed the world, affecting more than 180 countries. As a result, there has been considerable economic distress globally and a significant loss of life. Sadly, the vulnerable and immunocompromised in our societies seem to be more susceptible to severe COVID-19 complications. Global public health bodies and governments have ignited strategies and issued advisories on various handwashing and hygiene guidelines, social distancing strategies, and, in the most extreme cases, some countries have adopted “stay in place” or lockdown protocols to prevent COVID-19 spread. Notably, there are several significant risk factors for severe COVID-19 infection. These include the presence of poor nutritional status and pre-existing noncommunicable diseases (NCDs) such as diabetes mellitus, chronic lung diseases, cardiovascular diseases (CVD), obesity, and various other diseases that render the patient immunocompromised. These diseases are characterized by systemic inflammation, which may be a common feature of these NCDs, affecting patient outcomes against COVID-19. In this review, we discuss some of the anti-inflammatory therapies that are currently under investigation intended to dampen the cytokine storm of severe COVID-19 infections. Furthermore, nutritional status and the role of diet and lifestyle is considered, as it is known to affect patient outcomes in other severe infections and may play a role in COVID-19 infection. This review speculates the importance of nutrition as a mitigation strategy to support immune function amid the COVID-19 pandemic, identifying food groups and key nutrients of importance that may affect the outcomes of respiratory infections.
BACTERIAL (FREE LIVING AND PARTICLE BOUND) CELL- SIZE IN THE SURFACE WATERS OF RIVER CAUVERY AND ITS UPSTREAM TRIBUTARIES IN KARNATAKA STATE, INDIA

Cell-size here refers to mean length, as only the lengths of bacterial cells were measured in this study. Cell breadths were not measured, hence calculation of biovolume and biomass was not possible. In this study, the mean cell-lengths of heterotrophic bacterioplankton as well as their relation with environmental (water-quality) variables were analyzed for two years in the main river Cauvery and its four important upstream tributaries from February 2000 to January 2002. The initial hypothesis that all the five water courses have similar bacterial mean cell-lengths was rejected, because mean cell-lengths of free living and particle bound planktonic bacteria was more and was also significantly different in the river Lakshmanatheertha, when compared to the other four water courses studied. Season-wise grouped data revealed that, the mean cell-length of free-living bacteria was significantly less in winter season as compared to rainy and summer seasons during the second year of study only in the river Lakshmanatheertha. A correlation (r) analysis between the mean cell-lengths of heterotrophic bacteria and environmental (water-quality) variables revealed significant relations. Also with the help of regression analysis (r) the effect of some important environmental variables on the mean cell-length of heterotrophic bacteria has been discussed in the light of recent investigations in the field of fresh water microbial ecology.
Semiarid Crop Production from a Hydrological Perspective: Gap between Potential and Actual Yields

Rapid population growth in the dry climate regions, arable land scarcity, and irrigation expansion limitations direct our interest to possibilities of yield increase in rainfed agriculture. Literature, however, indicates large differences between actual and potential yields, and between yields on farmers’ fields and research stations. This article focuses on the determinants of these yield gaps and the windows of opportunity for yield increase on the farmer's field together with the agricultural challenges involved. The study links the conventional approach to estimate crop water requirements and dry spell effects on biomass production to a conceptual Green Water Crop Model. This model addresses the effects on crop yields of the sequential diversions of infiltrating rainfall (rainwater partitioning into runoff, plant available soil water, and deep percolation) and of different relations between nonproductive evaporation flow and productive transpiration flow, defined together as green water flow. Also, the effects of droughts and dry spells are analyzed. The model is used to demonstrate typical situations for semiarid and dry subhumid conditions (lengths of growing period (LGP) of 90 and 179 days, respectively) for maize (Zea mays (L.)) under on-station agricultural conditions. Based on detailed water flow analysis in a 3-year on-farm case study in the Sahel on pearl millet (Pennisetum glaucum (L.) Br. ), the model is used to clarify the large scope for improved yield levels, achievable through land and water management securing that runoff losses and deep percolation are reduced and nonproductive evaporation losses minimized. The analysis indicates that poor rainwater partitioning and low plant water uptake capacity alone reduces estimated on-farm grain yields to 1/10th of the potential yields. This suggests that lack of water per se not necessarily is the primary constraint to crop growth even in drought prone areas of sub-Saharan Africa. The conclusion is that even a doubling of crop yields would be agro-hydrologically possible with relatively small manipulations of rainwater partitioning in the water balance.
The influence of the radiation environment around the node on morphogenesis and growth of white clover (Trifolium repens)

The growth of clonally propagated clover plants (Trifolium repens) in the glasshouse was compared with their growth under canopies of Tropaeolum peregonium with or without supplementation from red light emitting diodes (LEDs) directed at the youngest node bearing a fully expanded leaf. The T. peregonium was grown over a net support so that its roots did not meet those of the clover. A similar experiment was set up in a permanent grassland using canopy shade from various grass species (Holcus lanatus, Lolium perenne and Agrostis stolonifera) with and without supplementation. The LEDs increased the ratio of red to far red radiation (R/FR) received by the treated node with no apparent increase in photosynthetically active radiation (PAR) during the day. The morphogenetic effects of canopy shading (notably fewer branched nodes and less well-developed branches) were largely removed by supplementation so that the supplemented plants came to resemble those grown in full daylight, particularly in the glasshouse experiments. The results highlight the Importance of the radiation environment at the base of the canopy in the morphogenetic responses of plants to putative competition, and the data are consistent with the hypothesis that responses are communicated acropetally along the stolon.
Effect of seed priming on growth and phenology of wheat under late-sown conditions

Seeds of four wheat varieties, Sonak, UP 2338, Raj 3765 and PBW 343, were treated with salts, water or growth regulator before sowing in the field in late winter. Sowing sprouted seeds resulted in more tillers, higher dry matter in leaves, stem and reproductive parts, and better grain yield. Priming with solutions of indole-3-acetic acid, KCl, water, ZnSO4 and Na2SO4 also gave more plant dry matter and grain yield than unprimed seeds. The plants from primed seeds took less time than the control to reach tillering, jointing, heading and flowering, but physiological maturity in all the treatments was at the same time. Biomass accumulation and grain yield were higher in UP 2338 and Raj 3765 in 1998–99, and in PBW 343 in 1999–2000. Copyright © 2004 Whurr Publishers Ltd
Temperature, metabolic rate, and constraints on locomotor performance in ectotherm vertebrates

Summary 1Adaptation to cold environments of ectotherm populations is expected to result in increased standard metabolic rates if resources are not limiting. However, it is still unclear how the maintenance of high standard metabolic rates would affect locomotor performance and its impact at the population level. 2We compared standard metabolic rate and swimming performance of Brown Trout populations inhabiting rivers with contrasting thermal conditions. To avoid environmental confounding effects, we tested offspring raised in controlled conditions. There were significant differences among populations and families for both traits, suggesting that the observed differentiation pattern has a genetic basis. 3We found a significant negative relationship between standard metabolic rate and swimming performance at the population level. On the other hand, standard metabolic rates tended to be negatively related to the temperature in the rivers of origin, whereas swimming performance was positively related to river temperature, but these trends were not significant. 4These results suggest that selection for traits positively linked with standard metabolic rates may result in lower relative prolonged swimming performance. The potential evolutionary consequences of the observed negative relationship are discussed.