Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of K+ Nutrition in Plants

Modern agriculture relies on mineral fertilization. Unlike other major macronutrients, potassium (K+) is not incorporated into organic matter but remains as soluble ion in the cell sap contributing up to 10% of the dry organic matter. Consequently, K+ constitutes a chief osmoticum to drive cellular expansion and organ movements, such as stomata aperture. Moreover, K+ transport is critical for the control of cytoplasmic and luminal pH in endosomes, regulation of membrane potential, and enzyme activity. Not surprisingly, plants have evolved a large ensemble of K+ transporters with defined functions in nutrient uptake by roots, storage in vacuoles, and ion translocation between tissues and organs. This review describes critical transport proteins governing K+ nutrition, their regulation, and coordinated activity, and summarizes our current understanding of signaling pathways activated by K+ starvation.
Modelling the transmission dynamics of Campylobacter in Ontario, Canada, assuming house flies, Musca domestica, are a mechanical vector of disease transmission

Campylobacter's complicated dynamics and multiple transmission routes have made it difficult to describe using a mathematical framework. Vector-borne disease transmission has been proposed as a potential transmission route of Campylobacter with house flies acting as a mechanical vector. This study aimed to (i) determine if a basic SIR compartment model that included flies as a mechanical vector and incorporated a seasonally forced environment compartment could be used to capture the observed disease dynamics in Ontario, Canada, and (ii) use this model to determine potential changes to campylobacteriosis incidence using predicted changes to fly population size and fly activity under multiple climate change scenarios. The model was fit to 1 year of data and validated against 8 and 12 years of data. It accurately captured the observed incidence. We then explored changes in human disease incidence under multiple climate change scenarios. When fly activity levels were at a 25% increase, our model predicted a 28.15% increase in incidence by 2050 using the medium–low emissions scenario and 30.20% increase using the high emissions scenario. This model demonstrates that the dynamics of Campylobacter transmission can be captured by a model that assumes that the primary transmission of the pathogen occurs via insect vectors.
HMGB1 as a Potential Biomarker and Therapeutic Target for Malignant Mesothelioma

Malignant mesothelioma (MM) is a rare, aggressive, and highly lethal cancer that is substantially induced by exposure to asbestos fibers. High-mobility group box 1 (HMGB1) is an intriguing proinflammatory molecule involved in MM. In this review, we describe the possible crucial roles of HMGB1 in carcinogenic mechanisms based on in vivo and in vitro experimental evidence and outline the clinical findings of epidemiological investigations regarding the possible roles of HMGB1 as a biomarker for MM. We conclude that novel strategies targeting HMGB1 may suppress MM cells and interfere with asbestos-induced inflammation.
Double Beta Decay Experiments With Loaded Liquid Scintillator

Liquid scintillator detectors have many properties which are highly advantageous for neutrino research at the MeV energy scale. There are various choices of additives to an organic liquid scintillator which typically function as the nuclear targets in a neutrino interaction. More recently, new techniques for loading a large amount of nuclei into liquid scintillator enable sensitive searches for double beta decay with various nuclei. KamLAND was an existing detector constructed for neutrino physics at the MeV scale, later upgraded to a sensitive detector for double beta decay (KamLAND-Zen). In this experiment, isotopically enriched xenon gas was loaded into the highly radiopure liquid scintillator. As xenon is a stable noble gas, the scintillation performance changes little, and it is safe for long-term use. To mitigate backgrounds, xenon was loaded only into the liquid scintillator in the central part of the detector, contained in a small nylon balloon. Owing to the large amount of xenon and low background level, the KamLAND-Zen experiment achieved the most sensitive search to date for neutrinoless double beta decay. The next phase of the experiment plans to increase the amount of xenon by almost double. In the future, sensitivity can be further enhanced by detector upgrades. SNO+ (successor to the Sudbury Neutrino Observatory) is a liquid scintillator detector that is currently being commissioned and will soon be taking data. Originally, SNO+ developed a technique to load neodymium into the liquid scintillator in order to conduct a double beta decay search with the isotope $^{150}$Nd. Later, SNO+ developed two new techniques that enable loading tellurium into liquid scintillator. The large natural abundance of $^{130}$Te and the ability to load tellurium at a significant concentration in the SNO+ liquid scintillator, while maintaining excellent scintillation optical properties, offer the prospect of a very sensitive search for neutrinoless double beta decay. This article will discuss both experiments, KamLAND-Zen and SNO+, highlighting the results achieved, the techniques involved in loading the liquid scintillator, and describing the purification of components of the loaded liquid scintillator to very low background levels.
The Eukaryotic Life on Microplastics in Brackish Ecosystems

Microplastics (MP) constitute a widespread contaminant all over the globe. Rivers and wastewater treatment plants (WWTP) transport annually several million tons of MP into freshwaters, estuaries and oceans, where they provide increasing artificial surfaces for microbial colonization. As knowledge on MP-attached communities is insufficient for brackish ecosystems, we conducted exposure experiments in the coastal Baltic Sea, an in-flowing river and a WWTP within the drainage basin. While reporting on prokaryotic and fungal communities from the same set-up previously, we focus here on the entire eukaryotic communities. Using high-throughput 18S rRNA gene sequencing, we analyzed the eukaryotes colonizing on two types of MP, polyethylene and polystyrene, and compared them to the ones in the surrounding water and on a natural surface (wood). More than 500 different taxa across almost all kingdoms of the eukaryotic tree of life were identified on MP, dominated by Alveolata, Metazoa, and Chloroplastida. The eukaryotic community composition on MP was significantly distinct from wood and the surrounding water, with overall lower diversity and the potentially harmful dinoflagellate Pfiesteria being enriched on MP. Co-occurrence networks, which include prokaryotic and eukaryotic taxa, hint at possibilities for dynamic microbial interactions on MP. This first report on total eukaryotic communities on MP in brackish environments highlights the complexity of MP-associated biofilms, potentially leading to altered microbial activities and hence changes in ecosystem functions.
Addressing Profiles of Systemic Inflammation Across the Different Clinical Phenotypes of Acutely Decompensated Cirrhosis

Background: Patients with acutely decompensated cirrhosis (AD) may or may not develop acute-on-chronic liver failure (ACLF). ACLF is characterized by high-grade systemic inflammation, organ failures (OF) and high short-term mortality. Although patients with AD cirrhosis exhibit distinct clinical phenotypes at baseline, they have low short-term mortality, unless ACLF develops during follow-up. Because little is known about the association of profile of systemic inflammation with clinical phenotypes of patients with AD cirrhosis, we aimed to investigate a battery of markers of systemic inflammation in these patients. Methods: Upon hospital admission baseline plasma levels of 15 markers (cytokines, chemokines, and oxidized albumin) were measured in 40 healthy controls, 39 compensated cirrhosis, 342 AD cirrhosis, and 161 ACLF. According to EASL-CLIF criteria, AD cirrhosis was divided into three distinct clinical phenotypes (AD-1: Creatinine<1.5, no HE, no OF; AD-2: creatinine 1.5–2, and or HE grade I/II, no OF; AD-3: Creatinine<1.5, no HE, non-renal OF). Results: Most markers were slightly abnormal in compensated cirrhosis, but markedly increased in AD. Patients with ACLF exhibited the largest number of abnormal markers, indicating “full-blown” systemic inflammation (all markers). AD-patients exhibited distinct systemic inflammation profiles across three different clinical phenotypes. In each phenotype, activation of systemic inflammation was only partial (30% of the markers). Mortality related to each clinical AD-phenotype was significantly lower than mortality associated with ACLF (p < 0.0001 by gray test). Among AD-patients baseline systemic inflammation (especially IL-8, IL-6, IL-1ra, HNA2 independently associated) was more intense in those who had poor 28-day outcomes (ACLF, death) than those who did not experience these outcomes. Conclusions: Although AD-patients exhibit distinct profiles of systemic inflammation depending on their clinical phenotypes, all these patients have only partial activation of systemic inflammation. However, those with the most extended baseline systemic inflammation had the highest the risk of ACLF development and death.
Triple Hop for Distance as a Predictor of Lower Extremity Performance in Firefighter Equipment

The objective of this study was to examine the predictive value of the Triple Hop for Distance (THD) physical performance test to assess lower extremity (LE) strength and power in individuals donning firefighter personal protective equipment (PPE). Thirty-one healthy participants completed the THD in firefighter PPE on both the dominant and non-dominant limb. Dependent variables included LE power (vertical jump height [cm]) on a jump mat, and LE strength of the quadriceps and hamstrings (peak torque [Nm]) on an isokinetic dynamometer. THD was a strong predictor of LE power on the dominant (p<0.01) and non-dominant (p<0.01) limbs. THD was also a moderate predictor of LE strength on both the dominant limb (Ham60 [p<0.01]), Quad60 [p<0.01]), and the non-dominant limb (Ham60 [p<0.01]), (Quad60 [p<0.01]). The THD was found to be a strong and valid predictor for clinical measures of LE power and strength in firefighter PPE.
Combining Immune Checkpoint Inhibitors: Established and Emerging Targets and Strategies to Improve Outcomes in Melanoma

The immune system employs several checkpoint pathways to regulate responses, maintain homeostasis and prevent self-reactivity and autoimmunity. Tumor cells can hijack these protective mechanisms to enable immune escape, cancer survival and proliferation. Blocking antibodies, designed to interfere with checkpoint molecules CTLA-4 and PD-1/PD-L1 and counteract these immune suppressive mechanisms, have shown significant success in promoting immune responses against cancer and can result in tumor regression in many patients. While inhibitors to CTLA-4 and the PD-1/PD-L1 axis are well-established for the clinical management of melanoma, many patients do not respond or develop resistance to these interventions. Concerted efforts have focused on combinations of approved therapies aiming to further augment positive outcomes and survival. While CTLA-4 and PD-1 are the most-extensively researched targets, results from pre-clinical studies and clinical trials indicate that novel agents, specific for checkpoints such as A2AR, LAG-3, IDO and others, may further contribute to the improvement of patient outcomes, most likely in combinations with anti-CTLA-4 or anti-PD-1 blockade. This review discusses the rationale for, and results to date of, the development of inhibitory immune checkpoint blockade combination therapies in melanoma. The clinical potential of new pipeline therapeutics, and possible future therapy design and directions that hold promise to significantly improve clinical prognosis compared with monotherapy, are discussed.
Evaluation of Shoulder Strength and Kinematics as Risk Factors for Shoulder Injury in United States Special Forces Personnel

Background: Musculoskeletal injuries at the shoulder are highly prevalent and place a large burden on United States Special Forces personnel. Literature is lacking regarding the risk factors for these types of injuries. Purpose/Hypothesis: The purpose of this study was to evaluate the association of shoulder strength and kinematic characteristics, which have shown retrospective associations with shoulder conditions/injuries, with prospectively collected shoulder injuries. We hypothesized that lower strength and abnormal kinematics would be predictive of future shoulder injury. Study Design: Case-control study; Level of evidence, 3. Methods: A total of 140 male Special Forces operators underwent a musculoskeletal evaluation of the shoulder that included a scapular kinematic assessment during a humeral elevation task and isokinetic strength testing of the scapular protractors/retractors, external/internal rotators, and elevators of the shoulder. From strength assessments, ipsilateral strength ratios and bilateral strength asymmetries were also calculated. Musculoskeletal injuries of the shoulder were collected prospectively by use of medical chart reviews at 365 days following the evaluation. Separate generalized estimating equations (GEEs) and simple logistic regressions were used to analyze the association between baseline predictors and development of shoulder injury. Results: Results of the GEEs showed no significant prediction of shoulder injury by shoulder strength (odds ratio [OR], 1.00-1.03), ipsilateral strength ratios (OR, 0.43-2.12), or scapular kinematics (OR, 0.99-1.01). Logistic regression indicated that none of the bilateral asymmetries were significantly predictive of shoulder injury (OR, 1.00-1.04). Conclusion: The results indicate that shoulder strength and kinematic characteristics are not risk factors for shoulder injury in the Special Forces population. These findings are in opposition to the general findings of previous research using a retrospective analysis.
Stable isotopic characterization of a coastal floodplain forest community: a case study for isotopic reconstruction of Mesozoic vertebrate assemblages

Stable isotopes are powerful tools for elucidating ecological trends in extant vertebrate communities, though their application to Mesozoic ecosystems is complicated by a lack of extant isotope data from comparable environments/ecosystems (e.g. coastal floodplain forest environments, lacking significant C4 plant components). We sampled 20 taxa across a broad phylogenetic, body size, and physiological scope from the Atchafalaya River Basin of Louisiana as an environmental analogue to the Late Cretaceous coastal floodplains of North America. Samples were analysed for stable carbon, oxygen and nitrogen isotope compositions from bioapatite and keratin tissues to test the degree of ecological resolution that can be determined in a system with similar environmental conditions, and using similar constraints, as those in many Mesozoic assemblages. Isotopic results suggest a broad overlap in resource use among taxa and considerable terrestrial–aquatic interchange, highlighting the challenges of ecological interpretation in C3 systems, particularly when lacking observational data for comparison. We also propose a modified oxygen isotope-temperature equation that uses mean endotherm and mean ectotherm isotope data to more precisely predict temperature when compared with measured Atchafalaya River water data. These results provide a critical isotopic baseline for coastal floodplain forests, and act as a framework for future studies of Mesozoic palaeoecology.
A Review of 3,4-methylenedioxymethamphetamine (MDMA)-Assisted Psychotherapy

This paper provides a brief review of the history, proposed pharmacological mechanisms, safety issues, and clinical applications of the medicine 3,4-methylenedioxymethamphetamine (MDMA). Most clinical MDMA research in patients to date has focused on MDMA-assisted psychotherapy to treat posttraumatic stress disorder (PTSD). In this review paper other potential therapeutic applications for MDMA therapy are described, including contemporary studies treating anxiety associated with autism and the authors' ongoing study exploring the potential role for MDMA-assisted psychotherapy to treat alcohol use disorder. MDMA therapy for PTSD is now entering the final Phase 3 stage of drug development, with a target set for licensing by the FDA and EMA in 2021. This means that if clinical efficacy criteria are achieved, MDMA would become a medicine.
Mouse models of porcine circovirus 2 infection

PCV2 is considered the main pathogen of porcine circovirus diseases and porcine circovirus‐associated diseases (PCVD/PCVAD). However, the exact mechanism underlying PCVD/PCVAD is currently unknown. Mouse models of PCV2 are valuable experimental tools that can shed light on the pathogenesis of infection and will enable the evaluation of antiviral agents and vaccine candidates. In this review, we discuss the current state of knowledge of mouse models used in PCV2 research that has been performed to date, highlighting their strengths and limitations, as well as prospects for future PCV2 studies.
Cataract in diabetes mellitus

Diabetes mellitus (DM) is a chronic systemic disease that has increases in prevalence over time. DM can affect all ocular structures, with cataract being the most common ocular complication. Cataract is the leading cause of blindness worldwide. Due to several mechanisms, there is an increased incidence of cataract formation in the diabetic population. Advancements in technology have now made cataract surgery a common and safe procedure. However, the diabetic population is still at risk of vision-threatening complications, such as diabetic macular edema (ME), postoperative ME, diabetic retinopathy progression, and posterior capsular opacification.
IT barometer 2003: survey of the Singapore construction industry and a comparison of results

SUMMARY: The IT Barometer 2003 has been conducted in Singapore, a country which aspires to become the IT and business hub of Asia. Singapore has an on-going IT development programme for the construction industry known as the CORENET which started in 1993. Several industry projects have been implemented to help the AEC firms adopt IT. The survey is a timely check on the levels of general adoption of IT and specific adoption of IT originating from the CORENET. The survey results of Singapore are compared with those of the countries surveyed, particularly, Denmark, Sweden and Finland, under the IT Barometer project. The response rate of the Singapore survey is 11.1% based on 84 returns from a total of 754 questionnaires mailed out. The data collected from the survey is analysed using the SPSS software version 11.0. The key findings on IT usage in Singapore and selected Nordic countries are summarised from the comparison. The four lessons for construction industries intending to adopt IT on a large scale are: (1) To avoid the “technology for the sake of technology” trap; (2) To develop standards, integrated databases and interactive applications; (3) Business strategy must support investments in information systems; and (4) To focus on people, their IT needs and ability to manage change.
Spatial analysis of public health facilities in Riyadh Governorate, Saudi Arabia: a GIS-based study to assess geographic variations of service provision and accessibility

Abstract Much attention has been given to address public health policy in Saudi Arabia, particularly for the management, quality, and coverage issues. However, assessment of spatial patterns, distribution, and provision of public health services has been neglected. This paper analyzes the availability and accessibility of public health facilities across Riyadh Governorate, Saudi Arabia at the subnational level. Spatial and attribute data of public health facilities potentially have been analyzed using GIS to produce accurate measure of accessibility. The spatial pattern of service distribution was examined using average nearest neighbor. Distances from demand points (populations) to providers (facilities) were calculated for each district using near analysis. In addition, the ratios of public health facility to population were calculated to identify underserved and overserved areas. The findings clearly indicate that the spatial pattern of the distribution of public health facilities was significantly clustered (p value < 0.001) with Z-score of −10.9. Several districts within the central parts of the governorate were identified as having a higher density of facilities, while most of districts that are located in the marginal parts exhibit very low density of health facilities. Overall, there was a considerable variation in the average distance from district centroids to health facilities. Substantially, less than half of the population (45.4%) living in 61 districts has access to public health facility within less than 1-km distance. In contrast, the greatest increase in distances was observed for 6% of population living in 38 districts. People that live in such districts need to travel long distances for public health care. The output of this analysis can assist policy-makers and authorities of Riyadh Governorate in planning public health delivery.
Genetic variation in Breviolum antillogorgium, a coral reef symbiont, in response to temperature and nutrients

Abstract Symbionts within the family Symbiodiniaceae are important on coral reefs because they provide significant amounts of carbon to many different reef species. The breakdown of this mutualism that occurs as a result of increasingly warmer ocean temperatures is a major threat to coral reef ecosystems globally. Recombination during sexual reproduction and high rates of somatic mutation can lead to increased genetic variation within symbiont species, which may provide the fuel for natural selection and adaptation. However, few studies have asked whether such variation in functional traits exists within these symbionts. We used several genotypes of two closely related species, Breviolum antillogorgium and B. minutum, to examine variation of traits related to symbiosis in response to increases in temperature or nitrogen availability in laboratory cultures. We found significant genetic variation within and among symbiont species in chlorophyll content, photosynthetic efficiency, and growth rate. Two genotypes showed decreases in traits in response to increased temperatures predicted by climate change, but one genotype responded positively. Similarly, some genotypes within a species responded positively to high‐nitrogen environments, such as those expected within hosts or eutrophication associated with global change, while other genotypes in the same species responded negatively, suggesting context‐dependency in the strength of mutualism. Such variation in traits implies that there is potential for natural selection on symbionts in response to temperature and nutrients, which could confer an adaptive advantage to the holobiont.