Miracle-dz/UniFAR-FTUs huggingface dataset:
Nutritionnal regulation of hepatic metabolism of amino acids in growing ruminant: consequences on supply of nitrogenous nutrients to the muscle.

The management of production efficiency is an important issue in meat producing ruminants. An improvement of the feeding allowances (including the ratio between nitrogen and energy supply) could increase the efficiency of nitrogen utilisation by animals. The splanchnic bed (portal drained viscera + liver) is in competition with the muscle for nitrogen utilisation because it represents only 6 % of body protein but contributes to 35% of whole body protein synthesis. The first aim of this thesis was to know the consequence of an imbalanced nitrogen (N) or energy (E) supply compared with a diet offering balanced supply necessary for growth (T) on the efficiency of amino acid (AA) utilisation by the splanchnic bed and the whole body of growing lambs. No (or small) alteration of nitrogen retention or growth was observed for the N fed animals compared with T, which led to an increased efficiency of nitrogen utilisation. On the contrary, lower nitrogen retention and growth were observed in E fed lambs. The second aim of this work was to determine which metabolic pathways were involved in the alterations of net hepatic AA removal. Labelled AA infusion in catheterised animals allowed the measurement of net fluxes of nutrients and irreversible loss rates of the labelled AA across the splanchnic bed (and hepatic export protein synthesis). In N fed lambs, the lower hepatic removal of AA was connected with a probable decreased hepatic catabolism of AA (except for branched chain AA) associated with a maintained protein synthesis, allowing an overall sparing of AA. On the opposite, in E fed lambs, an increased gluconeogenesis from AA and hepatic catabolism of AA (increased ureagenesis) could explain the lower nitrogen retention observed. The last aim of this thesis was to highlight some regulatory factors that could explain the metabolic adaptations observed in the splanchnic area. An ex vivo study using liver slices taken from lambs fed the 3 experimental diets, showed a higher efficiency for AA utilisation in all metabolic pathways using AA in N fed lambs. This might be related to a higher AA transport activity. On the other hand, with E diet, no significant difference was observed for protein synthesis, gluconeogenesis and oxidation compared with T, which means that external regulatory mechanisms (such as nutrients, hormones (insulin, glucagon)) are probably involved in the decrased hepatic protein synthesis observed in vivo. Thus, the nitrogen allowances should be re-evaluated for growing ruminants since our results suggest that INRA allowances overestimate the nitrogen requirements for lambs. On the opposite, energy allowances seem to fit well with the requirements and should not be reduced in order to maintain protein retention in animals.
DUSP11 Attenuates Lipopolysaccharide-Induced Macrophage Activation by Targeting TAK1

Key Points DUSP11 inhibits LPS-induced macrophage activation by dephosphorylating TAK1. Besides targeting RNAs, DUSP11 binds to and targets a protein substrate, TAK1. Dual-specificity phosphatase 11 (DUSP11, also named as PIR1) is a member of the atypical DUSP protein tyrosine phosphatase family. DUSP11 is only known to be an RNA phosphatase that regulates noncoding RNA stability. To date, the role of DUSP11 in immune cell signaling and immune responses remains unknown. In this study, we generated and characterized the immune cell functions of DUSP11-deficient mice. We identified TGF-β–activated kinase 1 (TAK1) as a DUSP11-targeted protein. DUSP11 interacted directly with TAK1, and the DUSP11–TAK1 interaction was enhanced by LPS stimulation in bone marrow–derived macrophages. DUSP11 deficiency enhanced the LPS-induced TAK1 phosphorylation and cytokine production in bone marrow–derived macrophages. Furthermore, DUSP11-deficient mice were more susceptible to LPS-induced endotoxic shock. The LPS-induced serum levels of IL-1β, TNF-α, and IL-6 were significantly elevated in DUSP11-deficient mice compared with those of wild-type mice. The data indicate that DUSP11 inhibits LPS-induced macrophage activation by targeting TAK1.
In Vivo NADH/NAD+ Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants

In planta NAD redox monitoring using a fluorescent protein sensor delivers insight into the in vivo dynamics of plant cytosolic redox metabolism. NADH and NAD+ are a ubiquitous cellular redox couple. Although the central role of NAD in plant metabolism and its regulatory role have been investigated extensively at the biochemical level, analyzing the subcellular redox dynamics of NAD in living plant tissues has been challenging. Here, we established live monitoring of NADH/NAD+ in plants using the genetically encoded fluorescent biosensor Peredox-mCherry. We established Peredox-mCherry lines of Arabidopsis (Arabidopsis thaliana) and validated the biophysical and biochemical properties of the sensor that are critical for in planta measurements, including specificity, pH stability, and reversibility. We generated an NAD redox atlas of the cytosol of living Arabidopsis seedlings that revealed pronounced differences in NAD redox status between different organs and tissues. Manipulating the metabolic status through dark-to-light transitions, respiratory inhibition, sugar supplementation, and elicitor exposure revealed a remarkable degree of plasticity of the cytosolic NAD redox status and demonstrated metabolic redox coupling between cell compartments in leaves. Finally, we used protein engineering to generate a sensor variant that expands the resolvable NAD redox range. In summary, we established a technique for in planta NAD redox monitoring to deliver important insight into the in vivo dynamics of plant cytosolic redox metabolism.
The Influence of the Trainer’s Social Behaviors on the Resilience, Anxiety, Stress, Depression and Eating Habits of Athletes

During their sporting lives, athletes must face multiple difficulties that can have consequences for their mental health and changes in their eating patterns. Therefore, the present study aims to analyze how social skills of the trainer influence the coping capacity, psychological well-being, and eating habits of the athlete, elements that are key to achieving success during competition. This study involved 1547 athletes and 127 trainer. In order to achieve the objective, the mean, standard deviation, bivariate correlations, reliability analysis and a structural equation model were analysed. The results showed that prosocial behaviours were positively related to resilience, while antisocial behaviours were negatively related. Resilience was negatively related to anxiety, stress and depression. Finally, anxiety, stress and depression were negatively related to healthy eating and positively related to unhealthy eating. These results highlight the importance of creating a positive social climate to develop coping strategies that promote mental health and healthy eating habits of athletes.
COVID-19 Associated Coagulopathy and Thrombotic Complications

The SARS-CoV-2 virus caused a global pandemic within weeks, causing hundreds of thousands of people infected. Many patients with severe COVID-19 present with coagulation abnormalities, including increase D-dimers and fibrinogen. This coagulopathy is associated with an increased risk of death. Furthermore, a substantial proportion of patients with severe COVID-19 develop sometimes unrecognized, venous, and arterial thromboembolic complications. A better understanding of COVID-19 pathophysiology, in particular hemostatic disorders, will help to choose appropriate treatment strategies. A rigorous thrombotic risk assessment and the implementation of a suitable anticoagulation strategy are required. We review here the characteristics of COVID-19 coagulation laboratory findings in affected patients, the incidence of thromboembolic events and their specificities, and potential therapeutic interventions.
Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil

Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances.
How Does Yoga Reduce Stress?, A Clinical Trial Testing Psychological Mechanisms.

Yoga interventions can reduce stress, but the mechanisms underlying that stress reduction remain largely unidentified. Understanding how yoga works is essential to optimizing interventions. The present study tested five potential psychosocial mechanisms (increased mindfulness, interoceptive awareness, spiritual well-being, self-compassion, and self-control) that have been proposed to explain yoga's impact on stress. Forty-two participants (62% female; 64% White) in a yoga program for stress reduction completed surveys at baseline (T1), mid-intervention (T2), and post-intervention (12 weeks; T3). We measured two aspects of stress, perceived stress and stress reactivity. Changes were assessed with paired t-tests; associations between changes in mechanisms were tested in residual change models. Only stress reactivity decreased, on average, from T1 to T3. Except for self-compassion, all psychosocial mechanisms increased from T1 to T3, with minimal changes from T2 to T3. Except for self-control, increases in each mechanism were strongly associated with decreases in both measures of stress between T1 and T2 and decreases in perceived stress from T1 to T3 (all p's<.05). Increased psychosocial resources are associated with stress reduction. Yoga interventions targeting these resources may show stronger stress reduction effects. Future research should test these linkages more rigorously using active comparison groups and larger samples. This article is protected by copyright. All rights reserved.
COVID-19 Pandemic: Psycho-social Consequences During the Social Distancing Period Among Najran City Population.

BACKGROUND COVID-19 is a global pandemic that endanger the health and enforced social distancing for the whole world. Social distancing may generate stress, anxiety, and depression. Understanding the psychosocial consequences of COVID19 during social distancing may help decision-makers to take suitable decisions that help in increasing awareness. Evaluate the psychosocial consequences of COVID-19 pandemic during the social distancing period and explore the relationship between social media use and psychological stress during COVID-19 outbreak among Najran city population. Research design is descriptive correlational research design. SUBJECTS AND METHODS A snowball sampling technique, was used to recruit participants live in Najran city during the COVID-19 pandemic (1508 participant). RESULTS A statistically significant differences (P<0.05) are observed between Saudi and non-Saudi participants in all social aspects assessed except for time spent on social media. In addition, a high mean of depression, stress, and anxiety subscale scores are observed in non-Saudi compared to the Saudi participants with statistically significant differences (p=0.000). As well as high DASS-21 total scores in non-Saudi compared to the Saudi participants. Also, there are positive statistically significant correlations (≤0.05) between participants' time spent in social media and their depression, stress, anxiety, and total DASS scores during the COVID-19 outbreak. CONCLUSION The findings of the present study indicate that COVID-19 pandemic generates stress, anxiety and depression among Najran population especially, non-Saudi. This poor psychological condition is exaggerated with prolonged social media use. COVID-19 also has negative impact on social wellbeing and use of social media cannot replace direct contact with friends. The current study results may be utilized to formulate interventions that enhance psychosocial health and resilience during the COVID-19 outbreak.
The Influence of Managerial Psychology on Job Satisfaction among Healthcare Employees in Ghana

Background: Employee job satisfaction has been established to be one of the important factors that work towards addressing the subject matter of productivity in organizations. Healthcare professionals deserve some level of basic psychological need satisfaction in the area of job autonomy. Reasons that lead to employees achieving job autonomy and job satisfaction have been researched by industrial and organizational psychologists but very few of such studies have directed their attention towards the role psychological capital can play. Therefore, this study sought to find out how much of an impact positive psychology can make on the job autonomy of healthcare employees leading to the fulfillment of job satisfaction. Methods: Data were collected from 385 healthcare professionals from the public sector. A structural equation model was performed to analyze the relationship that exists between the constructs of psychological capital and job autonomy leading to job satisfaction on the part of the employees. Results: Results showed both a direct and indirect positive relationship between hope and job satisfaction and indirect through job autonomy. Apart from self-efficacy, that had a very low positive relationship, optimism largely influenced job autonomy of healthcare professionals. Results also showed that psychological capital positively related to job autonomy while job autonomy minimally influenced job satisfaction. Conclusions: It is concluded from this study that healthcare professionals deserve some level of basic psychological need satisfaction in the area of job autonomy and that can stimulate positive work ethic.
Gamma Irradiation and Male Glossina austeni Mating Performance

Simple Summary African animal trypanosomosis, also known as nagana, is a neglected disease in South Africa, transmitted by tsetse flies. It has been proposed to manage this disease through the eradication of Glossina austeni, one of the vectors of this disease in South Africa. The strategy would be an integration of various control tactics and the release of sterilised colonised males (sterile insect technique) to eliminate relic pockets. The irradiated colonised males must be able to compete with wild males. In preparation of the technique, the mating performance of colony-reared male flies was assessed. Factors that can influence male mating performance, such as radiation dose, and the development stage that is exposed to radiation were evaluated in the laboratory and under semi-field conditions. Radiation doses of 80 Gy and 100 Gy induced 97–99% sterility in colony females that mated with colony males treated as adults or pupae. Walk-in field cage assessments indicated that a dose of up to 100 Gy did not adversely affect the mating performance of males irradiated as adults or late stage pupae. This study indicated that the colonized G. austeni males irradiated as adults or late stage pupae will be suited for the sterile insect technique. Abstract An area-wide integrated pest management (AW-IPM) strategy with a sterile insect technique (SIT) component has been proposed for the management of African animal trypanosomosis (AAT) in South Africa. In preparation for the SIT, the mating performance of colony reared Glossina austeni males under influencing factors such as radiation dose and the development stage that is exposed to radiation, was assessed under laboratory and semi-field conditions. The radiation sensitivity of G. austeni colonized 37 years ago when treated as adults and late-stage pupae was determined. Radiation doses of 80 Gy and 100 Gy induced 97–99% sterility in colony females that mated with colony males treated as adults or pupae. Males irradiated either as adults or pupae with a radiation dose of 100 Gy showed similar insemination ability and survival as untreated males. Walk-in field cage assessments indicated that a dose of up to 100 Gy did not adversely affect the mating performance of males irradiated as adults or late stage pupae. Males irradiated as adults formed mating pairs faster than fertile males and males irradiated as pupae. The mating performance studies indicated that the colonized G. austeni males irradiated as adults or late stage pupae will still be suited for SIT.
Effect of Varying Levels of Dietary Protein on the Growth and Body Composition of the Grey Mullet Liza macrolepis Fingerlings

Casein-gelatin based isocaloric purified diets with graded levels of protein (10-60%) were fed to the fingerlings of Liza macrolepis for 60 days to evaluate the growth, feed conversion and carcass composition. Relative growth, specific growth, normalized biomass index and survival rate were higher and feed conversion ratio, lower in fish which received 40% dietary protein. Carcass composition of the fish indicated that protein, lipid, carbohydrate and moisture were significantly different while ash content was not significantly different from the initial values. The studies showed that dietary protein at a level of 40% was optimal for the species.
Anti-Pig IGE and IGA Antibodies in Naive Primates and Nonhuman Primates With Pig Xenografts.

BACKGROUND Natural preformed anti-pig IgM/IgG antibodies in primates play an important role in xenograft rejection. As it is not clear how IgE and IgA engage in the immune system in xenotransplantation, we investigated natural preformed and elicited anti-pig IgE/IgA in naïve primates and after xenotransplantation in nonhuman primates. METHODS The binding of IgM/IgG/IgE/IgA antibodies to red blood cells (RBCs) from wild-type (WT), α1,3-galactosyltransferase gene-knockout (GTKO), and GTKO/cytidine monophospho-N-acetylneuraminic acid hydroxylase gene-knockout (CMAHKO)/β-1,4N-acetylgalactosaminyltransferase 2 gene-knockout (β4GalNT2KO) (i.e., triple-knockout pigs) pigs (p) were measured by flow cytometry in naïve human (n=50) and baboon (n=14) sera. Antibody binding to WT and GTKO pRBCs was also measured in the sera of baboons (non-sensitized n=7, sensitized n=2) and rhesus monkeys (non-sensitized n=2, sensitized n=11) following WT or GTKO pig organ/tissue xenotransplantation. Deposition of IgM/IgG/IgE/IgA in the grafts was detected by immunohistochemistry. RESULTS The majority humans had natural preformed IgM/IgG/IgE/IgA to WT and GTKO pRBCs. In contrast, IgM/IgG/IgE/IgA to TKO pRBCs were present at lower levels and frequency (p<0.01). Baboons also had IgM/IgG/IgE/IgA antibodies against WT pRBCs, but less to GTKO and TKO (p<0.01). After xenotransplantation into nonhuman primates, when IgM/IgG increased, IgE/IgA also increased, but to a lesser extent. In addition to IgM/IgG, IgE and/or IgA deposition was observed in rejected pig xenografts. CONCLUSIONS Primates develop serum anti-pig IgE/IgA antibodies both naturally and during xenograft rejection. The pathophysiological role, if any, of anti-pig IgE/IgA antibodies remains unknown.
The Association of Veterans' PSA Screening Rates with Changes in USPSTF Recommendations.

BACKGROUND In 2012, the United States Preventative Services Task Force (USPSTF) formally recommended against all Prostate Specific Antigen (PSA) screening for prostate cancer. Our goal was to characterize PSA screening trends in the Veterans Health Administration (VA) before and after the USPSTF recommendation, and to determine if PSA screening was more likely to be ordered based on a Veteran's race or age. METHODS Using the VA Corporate Data Warehouse, we created 10 annual groups of PSA-eligible men covering 2009-2018. We identified all PSA tests performed in the VA to determine yearly rates of PSA screening. All statistical tests were two-sided. RESULTS The overall rate of PSA testing in the VA decreased from 63.3% in 2009 to 51.2% in 2018 (p<.001). PSA screening rates varied markedly by age group during our study period, with men aged 70-80 having the highest initial rate and greatest decline (70.6% in 2009 to 48.4% in 2018, p<.001). Men aged 55-69 saw a smaller decline (65.2% in 2009 to 58.9% in 2018, p<.001) while the youngest men, aged 40-54, had an increase in PSA screening (26.2% in 2009 to 37.8 in 2018, p<.001). CONCLUSIONS In this analysis of PSA screening rates among veterans before and after the 2012 USPSTF recommendation against screening, we found that overall PSA screening decreased only modestly, continuing for more than half of the men in our study. Veterans of different races had similar screening rates, suggesting that VA care may minimize racial disparities. Veterans of varying age experienced significantly different trends in PSA screening.
Clinical usefulness of fully automated chemiluminescent immunoassay for quantitative antibody measurements in COVID‐19 patients

Since December 2019, we have been in the battlefield with a new threat to the humanity, known as severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), which causes coronavirus disease 2019 (COVID‐19), characterized by viral pneumonia. It may be asymptomatic or cause various symptoms, ranging from flu‐like symptoms to acute respiratory distress syndrome and eventually death. At present, the only reliable test for COVID‐19 diagnosis is quantitative reverse transcriptase‐polymerase chain reaction. Assessing the immune response against SARS‐CoV‐2 could increase the detection sensitivity of infected population. Hereby, we report the performances of a fully automated chemiluminescent immunoassay (CLIA) on 276 serum samples. One hundred samples obtained from COVID‐19 negative subjects (COVID‐19 free) were analyzed to evaluate the diagnostic specificity of antibody (Ab) detection. Thereafter, 176 samples obtained from 125 patients with confirmed COVID‐19 (COVID‐19 patients) were selected to assess the diagnostic sensitivity of the CLIA. All samples were analyzed on MAGLUMI 800 platform. All COVID‐19 free samples had Ab levels below the cutoff values. Hence, the diagnostic specificity was estimated at 100% (95% confidence interval [CI] = 96.3‐100.0; positive predictive value = 100%). By the 18th day from the onset of symptoms, we reached an optimal diagnostic sensitivity (more than 95.0%) In fact, the diagnostic sensitivity increased over time and between 15 and 25 days after symptoms onset, reached 95.5% (95% CI = 84.9‐99.2). The new automated CLIA analyzer appeared to be a robust and reliable method to measure specific Ab against COVID‐19 at high throughput. Our data suggest that combining Ab and nucleic acid detection could increase diagnostic sensitivity.