Miracle-dz/UniFAR-FTUs huggingface dataset:
Metagenomic insights into the characteristics of soil microbial communities in the decomposing biomass of Moso bamboo forests under different management practices

Introduction Considering the rapid growth and high biomass productivity, Moso bamboo (Phyllostachys edulis) has high carbon (C) sequestration potential, and different management practices can strongly modify its C pools. Soil microorganisms play an important role in C turnover through dead plant and microbial biomass degradation. To date, little is known about how different management practices affect microbial carbohydrate-active enzymes (CAZymes) and their responses to dead biomass degradation. Methods Based on metagenomics analysis, this study analyzed CAZymes in three comparable stands from each Moso bamboo plantation: undisturbed (M0), extensively managed (M1), and intensively managed (M2). Results The results showed that the number of CAZymes encoding plant-derived component degradation was higher than that encoding microbe-derived component degradation. Compared with the M0, the CAZyme families encoding plant-derived cellulose were significantly (p < 0.05) high in M2 and significantly (p < 0.05) low in M1. For microbe-derived components, the abundance of CAZymes involved in the bacterial-derived peptidoglycan was higher than that in fungal-derived components (chitin and glucans). Furthermore, M2 significantly increased the fungal-derived chitin and bacterial-derived peptidoglycan compared to M0, whereas M1 significantly decreased the fungal-derived glucans and significantly increased the bacterial-derived peptidoglycan. Four bacterial phyla (Acidobacteria, Actinobacteria, Proteobacteria, and Chloroflexi) mainly contributed to the degradation of C sources from the plant and microbial biomass. Redundancy analysis (RDA) and mantel test suggested the abundance of CAZyme encoding genes for plant and microbial biomass degradation are significantly correlated with soil pH, total P, and available K. Least Squares Path Modeling (PLS-PM) showed that management practices indirectly affect the CAZyme encoding genes associated with plant and microbial biomass degradation by regulating the soil pH and nutrients (total N and P), respectively. Discussion Our study established that M2 and M1 impact dead biomass decomposition and C turnover, contributing to decreased C accumulation and establishing that the bacterial community plays the main role in C turnover in bamboo plantations.
Effect of lifelong sucrose consumption at human-relevant levels on food intake and body composition of C57BL/6N mice

Introduction Controversies surround the issue if chronic consumption of a high-sugar diet is detrimental to health or not. This study investigates whether lifelong consumption of a higher sucrose diet will induce overeating, and obesity, and cause metabolic dysfunctions such as hyperglycemia and dyslipidaemia in C57BL/6N mice, compared to a lower sucrose diet. Methods Male C57BL/6N mice at 3 weeks of age were randomized into consuming a diet with 25 or 10% kcal from sucrose for the rest of their lives. Body weight, food and water intake, fasting blood glucose, insulin, and lipid levels were measured at regular intervals. At the end of the study, organs and tissues were collected and gene expression was measured. Results There was no discernible difference in the impact on food intake, body composition, glucose and lipid homeostasis, liver triglyceride content, life expectancy, as well as gene expression related to intermediary metabolism between mice fed a diet with 10 vs. 25% kcal as sucrose over their lifespan. We also showed that switching from a 25% kcal diet to a 10% kcal diet at different life stages, or vice versa, did not appear to affect these outcomes of interest. Discussion The results from our study suggest that lifelong consumption of a higher sugar diet generally did not induce overeating and obesity, disrupt carbohydrate metabolism and lipid homeostasis, and reduce life expectancy compared with a lower sugar diet. Our unorthodox findings disagreed with the popular belief that higher sugar consumption is detrimental to health, which should be confirmed in future studies.
Composition and assembly of the bacterial community in the overlying waters of the coral reef of China’s Xisha Islands

Coral reef ecosystems are one of the most diverse and productive habitats on Earth. Microbes in the reef-overlying waters are key players in maintaining this ecosystem through regulating biogeochemical and ecological processes. However, the composition structure and assembly mechanism of microbial community in the reef-overlying waters remain largely unknown. In the present study, the bacterial communities from the overlying waters of atolls and fringing reefs as well as the surface waters of the adjacent open ocean of the Xisha Islands in the South China Sea were investigated using 16S rRNA high-throughput sequencing combined with a size-fractionation strategy. The results showed that environments of all sampling stations were similar, characterized by an almost complete lack of inorganic nutrients such as nitrogen and phosphorus. Proteobacteria, Cyanobacteria and Bacteroidetes were the dominant phyla, and Synechococcus was most abundant at the genus level in both large fraction (LF; 1.6–200 μm) and small fraction (SF; 0.2–1.6 μm) communities. Only a slight difference in community composition between LF and SF samples was observed. The bacterial communities among the three habitat types showed noticeable differences, and the bacterial composition among the atoll reefs was more varied than that among the fringing reefs. The similarity of bacterial communities significantly declined with the increasing geographic distance, and stochastic processes were more important than deterministic processes in bacterial community assembly. This study sheds lights on the bacterial biodiversity of coral reefs and the importance of stochastic process in structuring bacterial communities.
Exploring in vitro expression and immune potency in mice using mRNA encoding the Plasmodium falciparum malaria antigen, CelTOS

The secreted malarial protein, Cell-Traversal protein for Ookinetes and Sporozoites (CelTOS), is highly conserved among Plasmodium species, and plays a role in the invasion of mosquito midgut cells and hepatocytes in the vertebrate host. CelTOS was identified as a potential protective antigen based on a proteomic analysis, which showed that CelTOS stimulated significant effector T cells producing IFN-γ in peripheral blood mononuclear cells (PBMCs) from radiation attenuated sporozoite-immunized, malaria-naïve human subjects. In a rodent malaria model, recombinant full-length CelTOS protein/adjuvant combinations induced sterile protection, and in several studies, functional antibodies were produced that had hepatocyte invasion inhibition and transmission-blocking activities. Despite some encouraging results, vaccine approaches using CelTOS will require improvement before it can be considered as an effective vaccine candidate. Here, we report on the use of mRNA vaccine technology to induce humoral and cell-mediated immune responses using this antigen. Several pfceltos encoding mRNA transcripts were assessed for the impact on protein translation levels in vitro. Protein coding sequences included those to evaluate the effects of signal sequence, N-glycosylation on translation, and of nucleoside substitutions. Using in vitro transfection experiments as a pre-screen, we assessed the quality of the expressed CelTOS target relative to the homogeneity, cellular localization, and durability of expression levels. Optimized mRNA transcripts, which demonstrated highest protein expression levels in vitro were selected for encapsulation in lipid nanoparticles (LNP) and used to immunize mice to assess for both humoral and cellular cytokine responses. Our findings indicate that mRNA transcripts encoding pfceltos while potent for inducing antigen-specific cellular cytokine responses in mice, were less able to mount PfCelTOS-specific antibody responses using a two-dose regimen. An additional booster dose was needed to overcome low seroconversion rates in mice. With respect to antibody fine specificities, N-glycosylation site mutated immunogens yielded lower immune responses, particularly to the N-terminus of the molecule. While it remains unclear the impact on CelTOS antigen as immunogen, this study highlights the need to optimize antigen design for vaccine development.
Status of E-ELT M5 scale-one demonstrator

The fifth mirror of the European Extremely Large Telescope optical train is a field stabilization tip/tilt unit responsible for correcting the dynamical tip and tilt caused mainly by wind load on the telescope. A scale-one prototype including the inclined support, the fixed frame and a basic control system was designed and manufactured by NTE-SENER (Spain) and CSEM (Switzerland) as part of the prototyping and design activities. All interfaces to the mirror have been reproduced on a dummy structure reproducing the inertial characteristics of the optical element. The M5 unit is required to have sufficient bandwidth for tip/tilt reference commands coming from the wavefront control system. Such a bandwidth can be achieved using local active damping loop to damp the low frequency mechanical modes before closing a position loop. Prototyping on the M5 unit has been undertaken in order to demonstrate the E-ELT control system architecture, concepts and development standards and to further study active damping strategies. The control system consists of two nested loops: a local damping loop and a position loop. The development of this control system was undertaken following the E-ELT control system development standards in order to determine their applicability and performance and includes hardware selection, communication, synchronization, configuration, and data logging. In this paper we present the current status of the prototype M5 control system and the latest results on the active damping control strategy, in particular the promising results obtained with the method of positive position feedback.
Endocannabinoid metabolism inhibition has no effect on spontaneous fear recovery or extinction resistance in Lister hooded rats

Endocannabinoid transmission is emerging as a target for treating anxiety-related disorders, given its regulation of fear extinction. Boosting anandamide levels via inhibition of its metabolism by fatty acid amide hydrolase (FAAH) can enhance extinction, whereas inhibiting monoacylglycerol lipase (MAGL) to elevate 2-arachidonoylglycerol levels can impair extinction. However, whether endocannabinoids regulate fear relapse over time or extinction resistance remains unclear. In two experiments using auditory fear conditioned rats, we examined the effects of the FAAH inhibitor URB597 and the MAGL inhibitor JZL184 administered systemically on 1) spontaneous fear recovery after delayed extinction, and 2) extinction resistance resulting from immediate extinction [the immediate extinction deficit (IED)]. In Experiment 1, URB597 or JZL184 was given immediately after delayed extinction occurring 24 h after conditioning. Extinction recall and spontaneous fear recovery were tested drug-free 1 and 21 days later, respectively. We found no effects of either drug on extinction recall or spontaneous fear recovery. In Experiment 2, URB597 or JZL184 was given before immediate extinction occurring 30 min after conditioning and extinction recall was tested drug-free the next day. We also examined the effects of propranolol, a beta-adrenoceptor antagonist that can rescue the IED, as a positive control. JZL184 enhanced fear expression and impaired extinction learning but we found no lasting effects of URB597 or JZL184 on cued extinction recall. Propranolol reduced fear expression but, unexpectedly, had no enduring effect on extinction recall. The results are discussed in relation to various methodological differences between previous studies examining endocannabinoid and adrenergic regulation of fear extinction.
Sediment load forecasting of Gobindsagar reservoir using machine learning techniques

With ever advancing computer technology in machine learning, sediment load prediction inside the reservoirs has been computed using various artificially intelligent techniques. The sediment load in the catchment region of Gobindsagar reservoir of India is forecasted in this study utilizing the data collected for years 1971–2003 using several models of intelligent algorithms. Firstly, multi-layered perceptron artificial neural network (MLP-ANN), basic recurrent neural network (RNN), and other RNN based models including long-short term memory (LSTM), and gated recurrent unit (GRU) are implemented to validate and predict the sediment load inside the reservoir. The proposed machine learning models are validated for Gobindsagar reservoir using three influencing factors on yearly basis [rainfall (Ra), water inflow (Iw), and the storage capacity (Cr)]. The results demonstrate that the suggested MLP-ANN, RNN, LSTM, and GRU models produce better results with maximum errors reduced from 24.6% to 8.05%, 7.52%, 1.77%, and 0.05% respectively. For future prediction of the sediment load for next 22 years, the influencing factors were first predicted for next 22 years using ETS forecasting model with the help of data collected for 33 years. Additionally, it was noted that each prediction’s error was lower than that of the reference model. Furthermore, it was concluded that the GRU model predicts better results than the reference model and its alternatives. Secondly, by comparing the prediction precision of all the machine learning models established in this study, it can be evidently shown that the LSTM and GRU models were superior to the MLP-ANN and RNN models. It is also observed that among all, the GRU took the best precision due to the highest R of 0.9654 and VAF of 91.7689%, and the lowest MAE of 0.7777, RMSE of 1.1522 and MAPE of 0.3786%. The superiority of GRU can also be ensured from Taylor’s diagram. Lastly, Garson’s algorithm and Olden’s algorithm for MLP-ANN, as well as the perturbation method for RNN, LSTM, and GRU models, are used to test the sensitivity analysis of each influencing factor in sediment load forecasting. The sediment load was discovered to be most sensitive to the annual rainfall.
Chinese university students’ conceptions of feedback and the relationships with self-regulated learning, self-efficacy, and English language achievement

In China, under the influence of examination-driven culture and teacher-centered ways of learning, students’ self-regulated learning (SRL) capabilities, self-efficacy, and actual English proficiency are greatly hindered. Given this situation, the Chinese Ministry of Education has promulgated the use of formative assessment in the College English curriculum at the tertiary level since 2004. Feedback, as an integrated part of formative assessment, takes up the largest proportion of the Chinese College English classroom assessment and intends to facilitate SRL and learning. However, whether feedback could facilitate students’ SRL and learning has not been fully investigated in this context in China. Therefore, this study first explored how students self-reported their conceptions of feedback, SRL, and self-efficacy, and second, the relationships among these constructs and their English language achievement in the College English course. A questionnaire was used to collect data on students’ conceptions of feedback, SRL, self-efficacy, and self-perceived English language proficiency. Their English test scores as an indicator of English language achievement were also collected. A total of 538 participants from a university in Northern China participated in this study. Data were analyzed using descriptive statistics, exploratory factor analyses, Pearson correlation analyses, and multiple regression analyses. The results found that Chinese students from the College English course reported a high level of conceptions of teacher and peer feedback, SRL, and self-efficacy, yet a low level of Teacher/Peer Feedback Ignored. For the relationships among these variables, students’ conceptions of feedback contributed to SRL and self-efficacy. Besides, self-efficacy was found to be the strongest predictor for self-perceived English language proficiency and standardized English test scores, both indicators for English language achievement. From the theoretical perspective, this study addressed the research gap in the literature by examining four constructs together, that is, students’ conceptions of feedback, SRL, self-efficacy, and English language achievement within a university context in China. From the pedagogical angle, the results can also support teachers in their feedback practices to facilitate students’ SRL, self-efficacy, and learning.
Smartphone addiction is more harmful to adolescents than Internet gaming disorder: Divergence in the impact of parenting styles

Background The adverse effects of smartphone addiction (SPA) and Internet gaming disorder (IGD) on adolescents’ mental health have been widely recognized. However, the influence of parenting styles on these high-risk Internet use behaviors of adolescents still remain elusive. Aiming to identify preventable patterns for adolescents with SPA or IGD, this study compared the mental health status between adolescents with SPA and IGD and used path analysis to confirm actual effects of parenting styles on SPA and IGD. Methods Participants were enrolled at a junior high school in Hunan Province and a senior high school in Shanxi Province, China [n = 3,049, female (male): 50.5% (49.5%), mean age = 15.68 ± 1.54]. All participants reported their socio-demographic characteristics and undertook standardized assessments of SPA, IGD, parenting styles, depression, anxiety, insomnia, self-control, and support utilization. Results High levels of parental care and low levels of parental overprotection benefited adolescents’ mental health with SPA and IGD. However, despite having a more positive parenting style, adolescents with only SPA showed more severe mental health problems than adolescents with only IGD. Furthermore, the results showed that the parenting style of encouraging autonomy might be a protective factor against IGD, but it might reinforce SPA indirectly by reducing abilities of support utilization and self-control in whole sample. Conclusion Compared to IGD, SPA which included different kinds of Internet addiction behaviors, was more hazardous for adolescents’ mental health. The divergent effects of an autonomy-encouraging parenting style on SPA and IGD may reflect the different impacts of self-control in different types of Internet addiction.
A cluster of Psittacosis cases in Lishui, Zhejiang Province, China, in 2021

Introduction Psittacosis, caused by Chlamydia psittaci, is widespread throughout the world. In humans, C. psittaci infection may lead to severe conditions and complications, including sepsis and multiple organ failure. We report a cluster of cases caused by C. psittaci in Zhejiang Province, 2021, which led to one death and three cases of hospitalization. Methods The cases were confirmed by nest-PCR, RT-PCR, and mNGS. Results The four cases were related and the sequences obtained from the samples were closely correlated with those from Taiwan. Discussion This study is the first to report on the case of death from psittacosis in Zhejiang Province, and our results help to assess the disease and recommend effective measures to prevent further spread of C. psittaci.
Perceptions of healthcare professionals and patients on the role of the pharmacist in TB management in Pakistan: A qualitative study

Background: Globally, tuberculosis (TB) is the second major cause of death from infectious diseases, particularly in developing countries. A multidisciplinary approach to the management of TB may help to curb the disease burden. Objective: The objective of this study was to outline the perceptions of healthcare professionals and patients regarding the potential role of pharmacists in TB management in Pakistan. Method: This was a large-scale qualitative study conducted at the Chest Disease Unit (CDU) of the Bahawal Victoria Hospital (BVH), Punjab, Pakistan. Data were collected through semi-structured interviews with physicians, pharmacists, and patients recruited using a mix of convenient and snowball sampling. The sample size was decided through standard saturation point criteria. All interviews were audio recorded and transcribed verbatim. The data were analyzed to draw conclusions using a thematic analysis approach. Results: Analysis of the data yielded 19 categories and seven themes. Physicians considered pharmacists qualified healthcare professionals, whereas patients considered them merely dispensers. Inventory management and dispensing of medicines were considered as major responsibilities of pharmacists. Physicians were extremely overburdened and wanted to delegate certain duties to pharmacists, subject to their prior extensive trainings. However, most of the physicians were unaware of the legal scope of pharmacy practice in Pakistan. With regard to the potential duties of pharmacists, physicians, pharmacists, and patients (patients—upon explaining the potential roles during the interview) endorsed monitoring, counseling, medicine brand selection, dose adjustment, inventory management, dispensing, and polypharmacy assessment as their potential roles. In view of all stakeholders, the rationale for integrating pharmacists in TB management included overburdened physicians, sub-standard patient care, medication safety issues, and patient dissatisfaction. The healthcare professionals highlighted that the major barriers to integrating pharmacists within the TB management system were limited interest of regulatory authorities and policy makers, followed by inadequate training and experience-driven questionable competency of pharmacists. Conclusion: The study participants acknowledged the potential role of pharmacists in TB management. However, it was emphasized that healthcare policy makers should devise strategies to overcome the underlying barriers before assigning medicine-related clinical roles to pharmacists.
The Collapse of Dense Star Clusters to Supermassive Black Holes: Binaries and Gravitational Radiation

The formation of binaries as a result of normal stellar-dynamic processes in dense clusters of compact stars is investigated analytically. The results of numerical simulations based on a simple homological model for the evolution of a cluster up to the point of catastrophic collapse are presented in extensive graphs and characterized in detail. It is shown that gravitational radiation begins to have a significant effect long before the onset of the high-redshift state. In the later stages, radiative dissipation from binary captures and flyby orbits acts to increase the final core mass that can undergo catastrophic collapse. Realistic initial conditions are found to lead to final cores of 100-100,000 solar masses, which can then collapse in a few dynamical time scales to form black holes 10-150 times larger. It is suggested that gravitational radiation from compact-star binaries may be detectable by ground-based interferometers. 55 references.