Miracle-dz/UniFAR-FTUs huggingface dataset:
temporal, daily variability of the human urinary and plasma metabolomes in a tightly controlled environment.

While metabolomics has tremendous potential for diagnostic biomarker and therapeutic target discovery, its utility may be diminished by the variability that occurs due to environmental exposures including diet and the influences of the human circadian rhythm. For successful translation of metabolomics findings into the clinical setting, it is necessary to exhaustively define the sources of metabolome variation. To address these issues and to measure the variability of urinary and plasma metabolomes throughout the day, we have undertaken a comprehensive inpatient study in which we have performed non- targeted metabolomics analysis of blood and urine in 26 volunteers (13 healthy subjects with no known disease and 13 healthy subjects with autosomal dominant polycystic kidney disease not taking medication). These individuals were evaluated in a clinical research facility on two separate occasions, over three days, while on a standardized, weight-based diet. Subjects provided pre- and post-prandial blood and urine samples at the same time of day, and all samples were analyzed by ‘‘fast lane’’ LC-MS-based global metabolomics. The largest source of variability in blood and urine metabolomes was attributable to technical issues such as sample preparation and analysis, and less variability was due to biological variables, meals, and time of day. Higher metabolome variability was observed after the morning as compared to the evening meal, yet day-to-day variability was minimal and urine metabolome variability was greater than that of blood. Thus we suggest that blood and urine are suitable biofluids for metabolomics studies, though nontargeted mass spectrometry alone may not offer sufficient precision to reveal subtle changes in the metabolome. Additional targeted analyses may be needed to support the data from nontargeted mass spectrometric analyses. In light of these findings, future metabolomics studies should consider these sources of variability to allow for appropriate metabolomics testing and reliable clinical translation of metabolomics data.
ComprehensiveDrug Screeningfor a PediatricPopulation

IC Ourcomprehensivedrugscreeninvolvescolonmetnc,immu-noassay, and chromatographic procedures. Here, we report our experience over 18 months with urine specimens from subjects one to 21 years old. Of 1454 specimens analyzed, 817(56%) were found to contain drugs. Those drugs present in more than 4% of the specimens were: acetaminophen, benzodiazepines, sympathomimetic amines, phencyclidine, salicylate, and phenobarbital. We further evaluated this population divided into four age groups. Acetaminophen was the most common drug in all age groups, but the prevalence of other drugs differed among groups. Anticonvulsant drugs pre-teen and illicitdrugs were more frequent in adolescent and young adult populations We discuss the implications of these data on drug screening in the pediatric population. The abuse, both licit Previous reports regarding type and frequency In retrospective study reviews the comprehensive drug-screening for and examines
Reclassification of the Candida haemulonii complex; C. 1 duobushaemulonii sp. nov. ( C. haemulonii group II) and C. 2 haemulonii var . vulnera var. nov.: Two multiresistant human 3 pathogenic yeasts. 4

: The Candida haemulonii species complex is currently known as C. haemulonii 18 groups I and II. Here we describe C. haemulonii group II as a new species, Candida 19 duobushaemulonii sp. nov. and C. haemulonii var . vulnera as new a variety of C. 20 haemulonii group I using phenotypic and molecular methods. These taxa and other 21 relatives of C. haemulonii (i.e. Candida auris and Candida pseudohaemulonii ) cannot be 22 differentiated by the presently used commercial methods for yeast identification. Four 23 isolates ( C. haemulonii var . vulnera ) differed from the other isolates of C. haemulonii in 24 the sequence of the internal transcribed spacer (ITS) regions of the nuclear ribosomal 25 RNA gene (nrRNA) operon (ITS). The new species and the new variety have a multi 26 resistant antifungal profile, which includes high MIC values for Amphotericin B 27 (Geometric mean: 1.18 mg/L for C. haemulonii var . vulnera and 2 mg/mL for C. 28 duobushaemulonii sp. nov) and cross resistance against azole compounds. 29 Identification of these species should be based on molecular methods, such as 30 sequence analysis of ITS and MALDI-TOF MS. 31 32 33 RPB1 BygDye version 3.1 and µl amplicon diluted. PCR were a first cycle of denaturation for 1 min at 95ºC, followed by 30 cycles of denaturation at 95ºC for 10 sec, annealing at 50ºC for sec, and elongation 60ºC for 4 min.
BIROn - Birkbeck Institutional Research Online Does structure

This paper examines the impact of ownership structure on Chinese mutual fund performance and market share. We focus on two dimensions of ownership structure, namely the background of the owners and the degree of ownership concentration. Using a hand-collected dataset comprising 731 observations for 94 fund management companies over the period from 2005 to 2015, we provide evidence with panel estimation shows that the government ownership ratio and government-controlled companies have a positive effect on funds’ performance. On the other hand, foreign ownership has a negative impact on performance and market share. Having a higher ownership concentration is more likely to increase the company’s market share, whereas government-controlled companies experience a negative impact on their market share.
Measurements of base pressure in the wake of a cylinder at low Reynolds numbers

Measurements have been made of the base pressure coefficients of long circular cylinders at low Reynolds numbers from 35 to 1100 in a cross flow. The variation of "base suction" (negative base pressure coefficient or - C_(pb)) with Reynolds number shows a remarkably good correspondence with the variation of the Strouhal number. It is also possible to relate the variation in suction with the physical modes of wake formation found in previous studies. It is shown that the level of base suction is affected in only a minor fashion by whether parallel or oblique shedding is present, and it is found that the data are independent of the base pressure hole sizes and cylinder diameters that were used. The base suction is essentially constant along the span of a cylinder (outside of regions extending about 20 diameters in from each end), and the results arc independent of cylinder aspect ratio (L/D) provided that one exceeds a critical value of L/D.
Mindfulness-based cognitive therapy combined with repetitive transracial magnetic stimulation (rTMS) on information processing and working memory of patients with multiple sclerosis

Background: MS is a demyelinating disease that can result in significant disability. Along with physical complications, this disease is associated with significant psychological complications, including cognitive decline. Therefore, this study aimed to determine the efficacy of mindfulness-based cognitive therapy in combination with rTMS on information processing and working memory in patients with MS. Methods: The current study used a single-case experimental design and included a follow-up (A-B-A). The statistical population of the present study was all MS patients in Tehran who referred to Imam Khomeini Hospital in Tehran in 2020. The present study sample consisted of 5 MS patients selected by the sampling methods available. Subjects were assessed three times before, during, and after the intervention using the Zahlen-Verbindongs and n-back tests in the two-back position. Subjects received cognitive therapy based on mindfulness and rTMS at a frequency of 10 Hz. Visual and graphical recovery percentage and effect size methods were used to analyze the data. Results: The current study's findings indicate that combining mindfulness with rTMS has a beneficial effect on the information processing and working memory of MS patients. Overall, 67.24% recovered following the intervention stage, 53.64% recovered following the follow-up for information processing, 104.04% recovered following the intervention stage, and 76.98% recovered following the follow-up for working memory. Conclusion: The study shows the effect of mindfulness combined with rTMS on cognitive problems in MS patients. Significant improvements in MS patients' information processing, working memory, and therapeutic outcomes were observed throughout the follow-up period.
Edinburgh Research Explorer These terrifying three words

These terrifying three words: A qualitative, mixed methods study of students' and mentors' Abstract Background: There is little empirical published research pertaining to fitness to practise and pre-registration nursing students. Much of the existing fitness to practise literature focusses on medical students and there is a preponderance of literature reviews and descriptive or discursive papers. Objectives: The multicentre study aimed to explore students’ and mentor’s understandings of fitness to practise processes in pre-registration nursing programmes. Design: A qualitative study in the interpretive paradigm with interpretive analysis involving 6 focus groups and 4 face to face interviews with nursing students and mentors. Setting: Eleven Higher Education Institutions providing pre-registration nursing education in the UK. Data were collected January 2014-March 2015 following ethical approval. Participants: Purposive sampling was used to recruit mentors and nursing (but not midwifery) students from pre-registration nursing programmes at different stages of educational preparation. Methods: Qualitatively driven semi-structured focus groups (n = 6) and interviews (n = 4) were conducted with a total of 35 participants (17 pre-registration nursing students and 18 nursing mentors). Results: Three themes identified from the student and mentor data are considered: Conceptualising Fitness to Practise; Good Health and Character; and Fear and Anxiety surrounding Fitness to Practise Processes. Conclusions: Uncertainty about understandings of fitness to practise contributed to a pervasive fear among students and reluctance among mentors to raise concerns about a student’s fitness to practise. Both students and mentors expressed considerable anxiety and engaged in catastrophic thinking about fitness to
Mathematical Programming and Game Theory for Decision Making

Mathematical Programming and Its Applications in Finance Linear Programs with Totally Unimodular Coefficient Matrix Interior Point Method for Convex Quadratic Programming Analysis of Sets of Constraints, Traveling Salesman Problem and Tolerance-Based Algorithms Pedigree Polytope One-Defective Vertex Coloring Problem Complementarity Problem Fuzzy Twin Support Vector Machines for Pattern Classification Minimum Sum of Absolute Errors Regression Hedging Against the Market with No Short Selling Mathematical Programming and Electrical Network Analysis Dynamic Optimal Control Policy Forecasting for Supply Chain and Portfolio Management Variational Analysis in Bilevel Programming Game Engineering Games of Connectivity Robust Feedback Nash Equilibrium De Facto Delegation and Proposer Rules Bargaining Set in Effectivity Function Dynamic Oligopoly as a Mixed Large Game -- Toy Market, Balanced Games, Market Equilibrium for Combinatorial Auctions and the Matching Core of Non-negative TU Games Continuity, Manifolds and Arrow's Social Choice Problem Mixture Class of Stochastic Games.
The Impact of Pregnancy and Phases of the Menstrual Cycle on Atopic Dermatitis

Objective: To investigate the impact of pregnancy and phases of the menstrual cycle on the symptoms of atopic dermatitis. Patients and Methods: Eighty female patients with atopic dermatitis were included in the study and interviews were performed. The total IgE level and the Eczema Area and Severity Index score of the patients were prospectively reviewed. Results: Eighty patients replied the questionnaire, and among them, 18 patients had completed at least 1 pregnancy. Among the 18 women who experienced pregnancy, 12 (66.7%) had noticed deterioration of their clinical symptoms during pregnancy. Of the 80 females, 30 (37.5%) patients had noticed deterioration of their AD as related to their menstrual cycle. For the patients who were sub-grouped as the intrinsic type of AD, the prevalence of symptom aggravation as related to pregnancy was significantly higher as compared to that of the extrinsic type of AD patients (p < 0.05). Conclusion: This suggests the relation of a hormonal negative influence on the clinical manifestations of AD.
Mediation of (cid:1) -Endorphin by Isoferulic Acid to Lower Plasma Glucose in Streptozotocin-Induced Diabetic Rats

We investigated the mechanism(s) by which isoferulic acid lowers plasma glucose levels in streptozotocin-induced diabetic rats (STZ-diabetic rats). In STZ-diabetic rats, isoferulic acid dose dependently lowered plasma glucose concentrations and increased plasma (cid:1) -endorphin-like immunoreactivity (BER). Both of these effects of isoferulic acid were abolished by pretreatment of rats with tamsulosin or 2-[2,6-dimethoxyphe-noxyethyl]aminomethyl-1,4-benzodioxane hydrochloride (WB 4101) at doses sufficient to block (cid:2) 1 -adrenoceptors. Also, isoferulic acid enhanced BER release from isolated rat adrenal medulla in a concentration-dependent manner that could be abolished by treatment with (cid:2) 1 -adrenoceptor antagonists. Moreover, bilateral adrenalectomy in STZ-diabetic rats elimi-nated the activities of isoferulic acid, including the plasma glucose-lowering effect and the plasma BER-elevating effect. Naloxone and naloxonazine inhibited the plasma glucose-low-ering activity of isoferulic acid at doses sufficient to block opioid (cid:3) -receptors. In contrast with the effect in wild-type diabetic mice, isoferulic failed to lower plasma glucose levels in opioid (cid:3) -receptor knockout diabetic mice. Treatment of STZ-diabetic rats with isoferulic acid three times in 1 day resulted in an increase in the expression of the glucose transporter subtype 4 form in soleus muscle. This effect was blocked by (cid:2) 1 -adrenoceptor or opioid (cid:3) -receptor antagonists. The re- duction of elevated mRNA or protein level of hepatic phosphoenolpyruvate carboxykinase was also impeded in the same groups of STZ-diabetic rats. In conclusion, our results suggest that isoferulic acid may activate (cid:2) 1 -adrenoceptors to enhance the secretion of (cid:1) -endorphin, which can stimulate the opioid (cid:3) -receptors to increase glucose use or/and reduce hepatic gluconeogenesis, resulting in a decrease of plasma glucose in STZ-diabetic rats.
The Disparity between Long-Term and Short-Term Forecasted Earnings Growth

We find the disparity between long-term and short-term analyst forecasted earnings growth is a robust predictor of future returns and revisions in long-term forecasted earnings growth. After adjusting for industry characteristics, stocks whose long-term earnings growth forecasts are far above or far below their implied short-term forecasts for earnings growth have negative and positive subsequent risk-adjusted returns, respectively. Despite the importance of conditioning on short-term forecasted earnings growth, these returns are not driven by earnings momentum. Instead, consistent with investors having limited attention, predictable revisions in long-term analyst forecasts appear to induce return predictability. JEL Classification: G12
A Triple Mutant in the Ω -loop of TEM-1 β -lactamase Changes the Substrate Profile via a Large Conformational Change and an Altered General Base for Catalysis

Background: TEM-1 β -lactamase hydrolyzes penicillins and early cephalosporins but not oxyimino-cephalosporins. Results: A TEM-1 triple mutant, W165Y/ E166Y/P167G, exhibits ceftazidime hydrolysis and a large active site conformational change. Conclusion: The mutant has an enlarged active site to accommodate ceftazidime and an alternative catalytic residue, Tyr166. Significance: The study reveals plasticity in β lactamase structure and mechanism in the evolution of altered substrate specificity. ABSTRACT β -Lactamases are bacterial enzymes that hydrolyze β -lactam antibiotics. TEM-1 is a prevalent plasmid-encoded β -lactamase in Gram-negative bacteria that efficiently catalyzes the hydrolysis of penicillins and early cephalosporins but not oxyimino-cephalosporins. A previous random in wider active-site
Essays in Macroeconomics

This dissertation consists of two chapters. The first chapter, titled ‘Growth and Welfare Implications of Sector-Specific Innovations,’ identifies the optimal government support of business research and development (R&D) activities when sectors are heterogeneous. To this end, I build an endogenous growth model in which firm R&D drives macroeconomic growth and firm dynamics in two sectors that have different characteristics: a consumption-goods sector and an investment-goods sector. I calibrate my model to U.S. data. To quantitatively characterize the optimal R&D subsidy rate for each sector, I simulate the economy with various R&D subsidy rates that range from 0 to 90 percent. By explicitly examining the transition path after a change in subsidy rates, I highlight the tradeoff between the consumption level in short-run and long-run growth. I find that the optimal combination of the subsidy rates is 82 percent in the consumption sector and 78 percent in the investment sector. By moving from the baseline subsidy rates (20 percent in each sector), society can achieve a 15 percent welfare gain in consumption-equivalent terms. The investment sector R&D subsidy generates three quarters of this welfare gain. The socially optimal subsidy system increases the annual GDP growth rate from 2 percent to 3.3 percent. I also find that when the government transfer budget is limited, the consumption sector R&D should be subsidized at a higher rate than the investment sector. The second chapter, ‘Worker Flows over the Life Cycle,’ is coauthored with Tomaz Cajner at the Federal Reserve Board and Toshihiko Mukoyama at the University of Virginia. Using Current Population Survey data, we document monthly movements across three labor market states (employment, unemployment, and out of labor market) and transitions across jobs during the life cycle of individuals. The data shows
GPPS-NA-2018-128 ON CLEAN INFLOW TESTING FOR INTERMEDIATE TURBINE DUCTS

In this paper, an investigation on the effect of different inlet conditions on an engine representative turbine centre frame (TCF) is presented. A comparison between three cases is obtained in the new high speed annular cascade rig at the Institute Thermal Turbomachinery and Machine Dynamics at Graz University of Technology. Differing from the engine realistic inlet conditions, a case with clean inflow, a case with IGVs and a case with IGVs and with spoke wheel are considered. Furthermore, a fourth inlet case is taken into account, namely the case with the unshrouded, uncooled HPT imposed inlet flow condition (rotating rig). A back-to-back comparison of the inlet and exit flow fields is based on time averaged data, obtained by five-hole-probe measurements. For the flow through the duct, the pressure rise coefficient at the duct hub and case as well as along the strut surface is evaluated. In addition, a state-of-the-art oil flow visualisation technique is used to determine the change of the wall shear stresses in the duct. CFD simulations also support the discussion and give additional insight into the flow behaviour.The major outcomes of the present study are that the investigated duct, not separating downstream of the
oxide synthase induces heat shock protein HSPA6 (HSP70B’) in human arterial smooth muscle cells

Endothelial nitric oxide synthase (eNOS) is the major source of nitric oxide (NO) production in blood vessels. One of the pleitropic functions of eNOS derived NO is to inhibit vascular smooth muscle cell proliferation in the blood vessel wall, and whose dysfunction is a primary cause of atherosclerosis and restenosis. In this study there was an interest in examining the gene profile of eNOS adenoviral (Ad-eNOS) transduced human coronary artery smooth muscle cells (HCASMC) to further understand the eNOS inhibitory effect on smooth muscle cell proliferation. To this aim a whole genome wide analysis of eNOS transduced HCASMCs was performed. A total of 19 genes were up regulated, and 31 genes down regulated in Ad-eNOS transduced HCASMCs compared to cells treated with an empty adenovirus. Noticeably, a cluster of HSP70 gene family members was amongst the genes up regulated. Quantitative PCR confirmed that transcripts for HSPA1A (HSP70A), HSPA1B (HSP70B) and HSPA6 (HSP70B’) were elevated 2, 1.7 and 14-fold respectively in Ad-eNOS treated cells. The novel gene HSPA6 was further explored as a potential mediator of eNOS signaling in HCASMC. Immunoblotting showed that HSPA6 protein was induced by AdeNOS. To functionally examine the effect of HSPA6 on SMCs, an adenovirus harboring the HSPA6 gene under the control of a constitutive promoter was generated. SMC proliferation 3 and 6 days post growth HSPA6 proliferation of has a similar effect, targeting vascular repair. Microarray experiments were carried out using the Affymetrix 2 platform using the human array HGU-133A (Affymetrix High Wycombe, UK) which represents ~22,000 genes. HCASMCs were cultured, transduced as above and kept in medium for 48 hrs. Microarray analysis was carried out on RNA isolated from three separate treatments, Ad-eNOS, Ad-Null transduced and non-transduced HCSMCs and each treatment was done in triplicate (n=3) such that there were a total of 9 treatment samples for RNA purification and microarray analysis. RNA was isolated as described below. RNA quality was assessed by resolution of 28S and 18S ribosomal RNA by gel electrophoresis and microanalysis using the bioanalyser analysis system. Transgene expression, and no expression in control treatments were confirmed in each sample by RT-PCR of the eNOS transcript. Data analysis was performed using GeneSpring Version 7.2 from Silicon Genetics. Lists of interesting genes were isolated based on filtering by fold change. Only a fold change of at least two or more at a particular time point was considered as a significant change.
Comparative Study IL-15 Induction by Different Viruses: A Up-Regulation of NK Cytotoxic Activity Via

IL-15 is a recently identiﬁed cytokine that belongs to the four a -helix bundle cytokine family and possesses biological activities similar to those of IL-2. Its ability to induce effectors of NK activity suggests its involvement in innate immunity. In this study, we analyzed the effect of different viruses (HSV, EBV, respiratory syncitial virus, vesicular stomatitis virus, inﬂuenza virus, reovirus, and Sendai virus) on the up-regulation of NK activity in vitro. Exposure of human PBMC to the these viruses resulted in an immediate up-regulation of NK activity of PBMC via IL-15 induction; this effect was abrogated in the presence of mAbs to IL-15. Results of experiments conducted in parallel using mAbs to IL-15, as well as to other cytokines (IL-2, IL-12, IFN- g , and TNF- a ), clearly indicated that IL-15 was speciﬁcally responsible for the observed effect. Furthermore, supernatants of virus-infected PBMC cultures signiﬁcantly enhanced NK activity of uninfected PBMC in vitro. An increase of IL-15 protein levels 20 h postin-fection was also conﬁrmed in a bioassay using the IL-2-dependent cell line CTLL. Kinetic analysis of IL-15 mRNA expression using a semiquantitative RT-PCR revealed that the level of IL-15 messages peaked at different time points (up to 12 h) postin-fection, depending on the nature of the virus. Taken together, these results suggest that the IL-15 response of the host to viral infection and the subsequent NK cell activation represent an important effector mechanism of the innate immune surveillance of the host against viral infections. The Journal of Immunology, 1999, 163: 4473–4480. Grabstein cell K562 The of of (50 (10 of at