Miracle-dz/UniFAR-FTUs huggingface dataset:
Growth cone localization of the mRNA encoding a chromatin regulator modulates neurite outgrowth

Neurons exploit mRNA localization and local translation to spatio-temporally regulate gene expression during development. Local translation and retrograde transport of transcription factors regulate nuclear gene expression in response to signaling events at distal neuronal ends. Whether epigenetic factors could also be involved in such regulation is not known. We report that the mRNA encoding the high mobility group N5 (HMGN5) chromatin binding protein localizes to growth cones of both neuronal-like cells and of hippocampal neurons. We show that Hmgn5 3’UTR drives growth cone localization and translation of a reporter gene, and that HMGN5 can be retrogradely transported into the nucleus along neurites. Loss of HMGN5 function induces transcriptional changes and impairs neurite outgrowth while HMGN5 overexpression induces neurite outgrowth and global chromatin decompaction. Interestingly, control of both neurite outgrowth and chromatin structure is dependent on proper growth cone localization of Hmgn5 mRNA. Our results provide the first evidence that mRNA localization and local translation might serve as a mechanism to couple the dynamic neuronal outgrowth process with chromatin regulation in the nucleus. Cells were primary PBS-0.1% secondary and was to to for F-actin. DAKO (DAKO). For RIPA on for and then centrifuged at 10 were on and microporous then signal was revealed with the ECL Prime Western Blotting Detection Reagent by autoradiography.
STRUCTURAL BASIS FOR MORPHEEIN-TYPE ALLOSTERIC REGULATION OF E. COLI GLUCOSAMINE-6P SYNTHASE: EQUILIBRIUM BETWEEN INACTIVE HEXAMER AND ACTIVE DIMER.

These authors to work. Background: E. coli GlmS is active as a dimer. Results: The dimer is in equilibrium with a hexameric state that is catalytically inactive in vitro . The glucosamine-6P product completely shifts the equilibrium towards the hexamer. Conclusion: This accounts for a morpheein-type allosteric regulation of E. coli GlmS activity. Significance: The inactive hexamer is a target for developing specific antibacterial agents. cultures (a, b and c) of HB101 E. coli strain were grown at 37°C in 2YT medium until OD 600 =1.8. 20 mM GlcN6P was added to b when OD 600 =0.5. The pellets from a 1 ml culture were resuspended in 500 µ l of buffer (20 mM Na-Phosphate pH 7.2, 1 mM EDTA, 2 mM DTT and anti proteases cocktail). 20 mM GlcN6P was included in the buffer for pellet c. After cell disruption, 7.5 µ l crude extract was loaded onto a NativePAGE TM gel run using Light Blue cathode buffer. 2 mM GlcN6P was added to the anode and cathode buffers. Lane d contains 0.1 µ g of purified recombinant GlmS. A lane including Native weight standards Apoferritin band 2 (480 kDa), β -phycoerythrin (242 kDa), Lactate Dehydrogenase (146 kDa), and Bovine Serum Albumin (66 kDa) was included in the gel. After electrotransfer, the membrane was stained with Ponceau S and the position of the standard markers, handmarked on the exposed film by transparency, is indicated on the left of the gel. C E. GlmS. The inactive dimer a transient form that is not stable in solution. morpheein 2mer(active) <=> 2mer* stable) <=>
Contribution of E-NTPDase1 (CD39) to renal protection from ischemia-reperfusion injury

Previous studies showed increased extracellular nucleotides during renal ischemia-reperfusion. While nucleotides represent the main source for extracellular adenosine and adenosine signaling contributes to renal protection from ischemia, we hypothesized a role for ecto-nucleoside-triphosphate-diphosphohydro-lases (E-NTPDases) in renal protection. We used a model of murine ischemia-reperfusion and in situ ischemic preconditioning (IP) via a hanging weight system for atraumatic renal artery occlusion. Initial studies with a nonspeciﬁc inhibitor of E-NTPDases (POM-1) revealed inhibition of renal protection by IP. We next pursued transcriptional responses of E-NTPDases (E-NTPDase1–3, and 8) to renal IP, and found a robust and selective induction of E-NTPDase1/CD39 transcript and protein. Moreover, based on clearance studies, plasma electrolytes, and renal tubular histology, IP protection was abolished in gene-targeted mice for cd39 whereas increased renal adenosine content with IP was attenuated. Furthermore, administration of apyrase reconstituted renal protection by apyrase of wild-type in increased renal a of renal protection from ischemia IP treatment. identify nucleotide phosphohydrolysis in renal
Estimate of void fraction and air entrainment flux in hydraulic jump using Froude number

Hydraulic jumps are induced in hydraulic facilities for the purposes of energy dissipation or flow aeration. Presently there is no means for a simple estimate of void fraction distribution and air entrainment flux, without detailed physical modelling. This paper presents a semi-theoretical model to simulate the void fraction and velocity distributions in hydraulic jumps characterised by partially-developed inflow conditions. Relationships were established between the inflow Froude number, jump roller length and key parameters that determine the full expression of void fraction and velocity profiles. The proposed model enables accurate prediction of void fraction, longitudinal velocity and air flux using the inflow Froude number. The results indicated considerable air flux contribution of free-surface aeration, in addition to the singular air entrainment at the jump toe, for moderate to large Froude numbers. A Froude number between 8 and 9 tended to achieve highest aeration rate with maximum total air flux.
Acute myocardial infarction complicated with takotsubo syndrome in an elderly patient: case report and literature review

1. Department of Cardiology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China; 2. Department of Cardiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China; 3. Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China The authors contributed equally to this manuscript ✉ Correspondence to: zpa00593@btch.edu. cn https://doi.org/10.11909/j.issn. 1671-5411.2022. 06.007
The Missing Middle: Filling the Gap Between Walkability and Observed Walking Behavior

Many fields of study recognize the interdependent health, environmental, and economic benefits of walking. To promote walking in entire populations, measures such as Walk Score have been developed to classify the walking friendliness or walkability of places. Yet high walkability is not always equated with increased walking. This paper investigates this discrepancy with the use of survey data on pedestrian behavior; a variety of geographic information system–derived land use and built environment measures of neighborhoods in Montreal, Quebec, Canada; and socioeconomic characteristics obtained from the 2011 National Household Survey. A descriptive analysis of walking behavior and neighborhood characteristics reveals that some neighborhoods with higher walking rates are characterized by a lower presence of parking lots and setbacks and a greater proportion of on-street tree canopy. Linear regressions predicting walking rates confirm these associations after adjusting for Walk Score and neighborhood socioeconomic characteristics. These findings suggest that more work is needed for nuancing walkability measures and offer particular insight for health professionals, planners, and engineers looking to promote walking as an alternative and healthier mode of transport. Reducing open space, such as parking lots and setbacks, and increasing street-level tree canopy are two ways that the urban built environment can be modified to support walking, especially in areas with high Walk Score and low walking rates.
Molecular Adjuvant Agonist of Human C5a Anaphylatoxin as a Response by a Conformationally Biased Induction of an Antigen-Specific CTL

A conformationally biased decapeptide agonist of human C5a anaphylatoxin (YSFKPMPLaR) was used as a molecular adjuvant in stimulating an Ag-speciﬁc CTL response against murine P815S target cells expressing an L d -restricted CTL epitope of the hepatitis B surface Ag (HBsAg). Groups of BALB/c mice (H-2 d ) were immunized with aqueous solutions of the HBsAg CTL epitopes ( IPQSLDSWWTSL and IPQSLDSWWTSL RR); the C5a agonist (YSFKPMPLaR); the C5a agonist and HBsAg CTL epitopes admixed ( IPQSLDSWWTSL and IPQSLDSWWTSL RR 1 YSFKPMPLaR); the C5a-active, HBsAg CTL epitope-C5a agonist constructs ( IPQSLDSWWTSL YSFKPMPLaR, IPQSLDSWWTSL RRYSFKPMPLaR, and IPQSLDSWWTSL RVRRYSF-PMPLaR); a C5a-inactive, reverse-moiety construct (YSFKPMPLaRRR IPQSLDSWWTSL ); and a C5a-attenuated, carboxyl-ter-minal-blocked construct ( IPQSLDSWWTSL RRYSFKPMPLaRG). Ag-speciﬁc CD8 1 CTL responses were observed after the secondary boost in the absence of any added adjuvant only in mice that were immunized with C5a-active contructs, IPQSLDSWWTSL RRYSFKPMPLaR and IPQSLDSWWTSL RVRRYSFKPMPLaR. These two C5a-active immunogens contained potential subtilisin-sensitive Abs to the HBsAg CTL epitope or the C5a agonist are not produced by immunization with the HBsAg CTL epitope-C5a agonist constructs
Evaluation Of The Impact Of Standing Support On Ground Behavior In Longwall Tailgates

Longwall mines typically use some form of standing support for secondary roof support in longwall tailgate entries. Although there have been several new support products developed for this application, there remains no universal design criteria to optimize the application of these support technologies. The requirement for optimization and proper support selection is to understand the degree of control that the support has on the ground behavior. The ground reaction curve and numerical modeling was used to evaluate the impact of standing support on ground behavior. LaModel was used to evaluate the impact of standing support on main roof and floor behavior and pillar yielding. The conclusion drawn from this study was that standing supports do not have sufficient capacity to control main roof or floor loading or prevent the resulting convergence of the tailgate entry. However, it is imperative that this “uncontrollable convergence” be considered in the support design to prevent premature failure of the support. A FLAC model was used to evaluate the near-seam roof and floor behavior in conjunction with the global vertical and horizontal stresses. The model suggests that standing roof supports can have some impact on the ground behavior as the elastic response of the rock is exceeded and rock structure deteriorates from the stress concentrations that develop around the tailgate opening. During this phase, the capacity and stiffness of the standing support can be critical to the stability of the opening, as eventually the rock mass will be transformed into a partially detached structure whose weight must be supported by the standing support. This work is part of a ground control program of research at the National Institute for Occupational Safety and Health (NIOSH) aimed at improving mine safety by reducing roof fall injuries and fatalities.
High‐quality ice plant reference genome analysis provides insights into genome evolution and allows exploration of genes involved in the transition from C3 to CAM pathways

Summary Ice plant (Mesembryanthemum crystallinum), a member of the Aizoaceae family, is a typical halophyte crop and a model plant for studying the mechanism of transition from C3 photosynthesis to crassulacean acid metabolism (CAM). Here, we report a high‐quality chromosome‐level ice plant genome sequence. This 98.05% genome sequence is anchored to nine chromosomes, with a total length of 377.97 Mb and an N50 scaffold of 40.45 Mb. Almost half of the genome (48.04%) is composed of repetitive sequences, and 24 234 genes have been annotated. Subsequent to the ancient whole‐genome triplication (WGT) that occurred in eudicots, there has been no recent whole‐genome duplication (WGD) or WGT in ice plants. However, we detected a novel WGT event that occurred in the same order in Simmondsia chinensis, which was previously overlooked. Our findings revealed that ice plants have undergone chromosome rearrangements and gene removal during evolution. Combined with transcriptome and comparative genomic data and expression verification, we identified several key genes involved in the CAM pathway and constructed a comprehensive network. As the first genome of the Aizoaceae family to be released, this report will provide a rich data resource for comparative and functional genomic studies of Aizoaceae, especially for studies on salt tolerance and C3‐to‐CAM transitions to improve crop yield and resistance.
Impact of Propofol-based Total Intravenous Anesthesia Versus Inhalation Anesthesia on Long-term Survival After Cancer Surgery in a Nationwide Cohort

Objective: To compare the impact of propofol-based total intravenous anesthesia (TIVA) versus inhalational anesthesia (IA) on the overall survival following cancer surgery. Background: The association between intraoperative anesthetics and patients’ long-term outcomes following cancer surgery remains controversial. Methods: This retrospective cohort study used nationwide data from the Korean National Health Insurance Service. Adult patients who underwent cancer resection surgery (breast, gastric, lung, liver, kidney, colorectal, pancreatic, esophageal, and bladder cancer) under general anesthesia between January 2007 and December 2016 were included. Patients were divided into propofol-based TIVA or IA groups according to the type of anesthesia received. A total of 312,985 patients (37,063 in the propofol-based TIVA group and 275,922 patients in the IA group) were eligible for analysis. The primary outcome was the comparison of overall survival following surgery between the groups in each cancer type. We compared the all-cause mortality between the 2 groups, stratified by cancer type using time-dependent Cox regression after propensity score–based inverse probability of treatment weighting. We further examined the comparison of overall survival in a meta-analysis using data from our study and previously published data comparing propofol-based TIVA with IA after cancer surgery. Results: The number of deaths in the propofol-based TIVA and IA groups was 5037 (13.6%) and 45,904 (16.6%), respectively; the median (interquartile range) follow-up duration was 1192 (637–2011) days. Multivariable Cox proportional hazards regression analysis revealed no significant association between the type of general anesthesia and overall survival after cancer surgery in the weighted cohort for each cancer type (all P>0.05) and for total population [adjusted hazard ratio (HR): 0.98, 95% confidence interval (CI): 0.93–1.04]. In a meta-analysis, single-center studies showed higher overall survival in the TIVA group than in the IA group (pooled adjusted HR: 0.65, 95% CI: 0.47–0.91, P=0.01), while multicenter studies showed insignificant pooled adjusted HRs (pooled adjusted HR: 1.05, 95% CI: 0.82–1.33, P=0.71). Conclusions: There is no association between the type of general anesthesia used during cancer surgery and postoperative overall, 1-, and 5-year survival.
Prevalence of and factors associated with swellings of the ribs in tie stall housed dairy cows in Germany

Swellings of the ribs result from severe injury and affected animals are subjected to considerable and prolonged pain and suffering. The knowledge on rib swellings in dairy cows has yet been very limited. Therefore, the present study aimed at determining the prevalence of rib swellings in tie stall housed dairy cows in Germany as well as at identifying associated factors. Mean animal-level prevalence of rib swellings for 2,134 cows was 7.54% with a mean of 7.00% on farm level (range 0.00% - 37.49%). Multivariable mixed logistic regression models including nested random effects were built and factors associated with swellings of the ribs were evaluated for 1,740 dairy cows on 96 farms in Germany. Out of the initial 22 predictors, 8 factors were selected for the final model. Managing dairy cows on a part-time basis (OR 0.49 [CI 0.25–0.98]) appeared to decrease the odds for rib swellings compared with full-time farming. Cattle breeds other than Simmental entailed lower odds for rib swellings (OR 0.29 [CI 0.14–0.59]). Lame cows (OR 2.59 [CI 1.71–3.93]) and cows with wounds and/or swellings of the hocks (OR 2.77 [CI 1.32–5.84]) had more than two times the odds for rib swellings compared with sound animals. The results of the present study can help raising awareness of rib swellings in dairy cows and contribute to the body of evidence on this condition.
Prolonged NHE Activation may be Both Cause and Outcome of Cytokine Release Syndrome in COVID-19.

The release of cytokines and chemokines such as IL-1β, IL-2, IL-6, IL-7, IL-10, TNF-α, IFN-γ, CCL2, CCL3, and CXCL10 is increased in critically ill patients with COVID-19. Excessive cytokine release during COVID-19 is related to increased morbidity and mortality. Several mechanisms are put forward for cytokine release syndrome during COVID-19. Here we would mention a novel pathways. SARS-CoV-2 increases angiotensin II levels by rendering ACE2 nonfunctional. Angiotensin II causes cytokine release via AT1 and AT2 receptors. Moreover, angiotensin II potently stimulates the Na+/H+ exchanger (NHE). It is a pump found in the membranes of many cells that pumps Na+ inward and H+ outward. NHE has nine isoforms. NHE1 is the most common isoform found in endothelial cells and many cells. NHE is involved in keeping the intracellular pH within physiological limits. When the intracellular pH is acidic, NHE is activated, bringing the intracellular pH to physiological levels, then its activity ends. Sustained NHE activity is highly pathological and causes many problems. Prolonged NHE activation in COVID-19 may cause a decrease in intracellular pH through H+ ion accumulation in the extracellular area and subsequent redox reactions. The activation reduces the intracellular K+ concentration and leads to Na+ and Ca2+ overload. Increased ROS can cause intense cytokine release by stimulating NF-κB and NLRP3 inflammasomes. Cytokines also cause overstimulation of NHE. As the intracellular pH decreases, SARS-CoV-2 rapidly infects new cells, increasing the viral load. This vicious circle increases morbidity and mortality in patients with COVID-19. On the other hand, SARS-CoV-2 interaction with NHE3 in intestinal tissue is different from other tissues. SARS-CoV-2 can trigger CRS via NHE3 inhibition by disrupting the intestinal microbiota. This review aimed to help develop new treatment models against SARS-CoV-2-induced CRS by revealing the possible effects of SARS-CoV-2 on the NHE.
Windrowing and fuel-chip quality of residual forest biomasses in northern Sweden

ABSTRACT Utilization of forest residual biomasses, including logging residues (LR) and whole tree-parts (WT), in Fennoscandia is expected to increase in response to increases in demand from the bioeconomy. LR and WT are often seasoned in windrows at roadsides, and more knowledge (prior to comminution) of their dry mass content would be highly useful for logistic planning and value estimations. Therefore, we have described and compared windrow fuel-chip quality and storage conditions of LR and WT delivered in one season to the same energy plant by the same supplier. A total of 76 windrows in northern Sweden were surveyed and chipped and 25 were also sampled to assess their moisture content (MC), ash content (AC) and particle size distribution. The mean MC (45%) did not significantly differ between LR and WT, but varied substantially within and between sites. LR fuel-chips had higher AC than WT (2.4% vs. 1.5%) and two-fold higher proportions of fines and oversized fractions (12.2 vs. 5.8% and 2.2 vs. 1.1%, respectively). LR windrows fully exposed to ambient conditions had 6.7% lower MC (43%) than sheltered counterparts. Average bulk densities of LR and WT were 66 and 59 dry kg per bulk cubic meter, respectively. The results enable development of models for estimating LR or WT windrows’ dry mass contents, show effects of some storage conditions on MC, and highlight the need for holistic supply chain management for cost-effective delivery of high-quality residual forest biomasses.
Characterizing Youth-Caregiver Concordance and Discrepancies in Psychopathology Symptoms in a US Community Sample

Abstract Evidence shows that reports of psychopathology symptoms by youth and their caregiver informants differ. To quantify youth-caregiver discrepancies in psychopathology symptoms and factors associated with such discrepancies, we investigated differences in how youth and their caregivers rated psychopathology symptoms. The sample (N = 5094) was extracted from the Philadelphia Neurodevelopmental Cohort, a community-based sample of youth and included participants ages 11-17 years old with both youth and caregiver reported symptom scores. Across psychopathology symptoms, youth-caregiver concordance was poor to fair (Cohens kappa for symptom items ranged between 0.03–0.41). Psychosis symptoms had the lowest concordance—Cohen’s kappa ranged from 0.03 to 0.17 across psychosis symptoms. Discrepancies between youth and caregiver symptom reports were greater than average for Black youth and for youth of low socioeconomic status; discrepancies were also higher than average in youth with any psychiatric disorder when compared to typically developing youth. Network analysis of difference scores obtained by subtracting youth symptom scores from caregiver reported symptom scores showed that network connectivity (i.e., correlated difference scores) was sparsest for psychosis spectrum compared to other psychiatric disorders. Using a large sample, we show that youth and their caregiver informants tend to report psychopathology symptoms differently. Youth-caregiver discrepancies were the most pronounced for Black youth and youth of low socio-economic status. Race and socioeconomic status contribute to significant differences in how youth and their caregivers report such symptoms and are important factors that should be accounted for to facilitate accurate mental health symptom assessment and evaluation.
Charge transfer and metal–insulator transition in (CrO2) m /(TaO2) n superlattices

Various interfacial emergent phenomena have been discovered in tunable nanoscale materials, especially in artificially designed epitaxial superlattices. In conjunction, the atomically fabricated superlattices have exhibited a plethora of exceptional properties compared to either bulk materials separately. Here, the (CrO2) m /(TaO2) n superlattices composed of two lattice-matched metallic metal oxides are constructed. With the help of first-principle density-functional theory calculations, a computational and theoretical study of (CrO2) m /(TaO2) n superlattices manifests the interfacial electronic properties in detail. The results suggest that emergent properties result from the charge transfer from the TaO2 to CrO2 layers. At two special ratios of 1:1 and 1:2 between m and n, the superlattices undergo metal-to-insulator transition. Additionally, the bands below the Fermi level become narrower with the increasing thickness of the CrO2 and TaO2 layers. The study reveals that the electronic reconstruction at the interface of two metallic materials can generate interesting physics, which points the direction for the manipulation of functionalities in artificial superlattices or heterostructures within a few atomic layers.