Miracle-dz/UniFAR-FTUs huggingface dataset:
High Expression of p21 as a Potential Therapeutic Target in Ovarian Clear-cell Carcinoma

Background/Aim: DNA damage response (DDR), wherein p21 is a cell fate determinant, is a potential cancer therapeutic target. Molecular expression during DDR was explored in ovarian clear-cell carcinoma (CCC). Materials and Methods: CHK1, CHK2, TP53 and p21 expression in DDR was examined using immunostaining in surgical sections of CCC (n=22). Molecular alterations in two types of CCC cell lines, JHOC-5 and JHOC-9, were investigated using western blot analysis. Results: Expression of DDR-associated molecules was noted in most patients. While high p21 expression was found in half of the patients, the remaining patients exhibited low p21 expression. Treatment with UC2288, a p21 inhibitor, attenuated proliferation of both cell lines, more prominently in JHOC-9, resulting in reduced viability and subsequent apoptosis. Conclusion: p21 Inhibitor induced cell death in cells with high p21 expression, suggesting that p21 suppression can be a therapeutic strategy to treat patients with CCC.
Impact of Physician-Pharmacist Collaboration on Diabetes Outcomes and Health Care Use

Objective: To evaluate the impact of physician–pharmacist collaboration for disease-state management on diabetes outcomes in primary care by comparing outcomes between physician-managed care and pharmacist collaborative care. Methods: A retrospective, observational cohort study was conducted at Ascension Medical Group Via Christi, P.A. from January 1, 2016 to June 30, 2018. Health outcomes were analyzed in 385 patients with diabetes mellitus collaboratively managed by a physician and pharmacist (collaborative care group). Similar patients managed by physician only (usual care group) were matched to the collaborative care group using nearest neighbor matching. The primary outcome compared glycosylated hemoglobin (HbA1c) change between collaborative care and usual care groups at 12 months. Results: The mean change in HbA1c decreased by 1.75% in the collaborative care group and 0.16% in the usual care group (P < .0001). The usual care group had a larger number of patients with HbA1c less than 8% at follow-up (P = .0049). Additional outcomes included decrease in total cholesterol (P = .0023), triglycerides (P = .0016), and an increase in PPSV23 pneumococcal vaccinations (P = .0255) in the collaborative care group. The usual care group had an increase in PCV13 pneumococcal vaccinations (P = .0075). Both emergency department visits (P = .0162) and hospitalizations (P = .0225) decreased significantly in the collaborative care group, estimating total savings of $633,015. Conclusions: The collaboration of pharmacists and physicians in the primary care setting is associated with improved diabetes outcomes and substantially reduces costs related to decreased health care use.
Handbook of marine microalgae : biotechnology advances

Handbook of Microalgae: Biotechnology Advances offers complete coverage of marine microalgae, including biology, production techniques, biotechnological applications, economic perspectives of applications, and environmental effects of marine microalgae blooms. With contributions from world experts, Handbook of Microalgae: Biotechnology Advances focuses on microalgae from an organism perspective to offer a complete picture from evolution to biofuel. * Focuses on a comprehensive approach from an organism point of view* Contains full coverage of all aspects of microalgae from biology through biotechnological and biomedical applications* Includes biological properties of commercial algal species* Provides microalgae screening and identification methods, culturing methods and new aspects of processing
Evaluation of the Antioxidant Activities of Psidium guajava and Aloe vera

This paper aimed to study the antioxidant properties of two Nigerian plants Psidium guajava (guava) Myrtaceae and Aloe vera Liliaceae plants which have a broad application in phytomedicine. The plants were assessed by quantifying their individual chemical contents and their 1:1 (mass/mass) homogenous combination (guava+A. vera) simultaneously. The non-antioxidant phytochemical quantified included total alkaloids. There was a significant difference in the total alkaloids content (measure on dry weight basis, mg/g) in the analysed plant materials in the order of guava (111.13±0.45)>guava+A. guava (65.99±0.37)>A. vera (22.86±0.15). The antioxidant properties measured were the levels of total phenol, tannin, total flavonoid, total saponin, vitamin C, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability and trolox equivalent antioxidant capacity (TEAC). P. guajava recorded significantly higher (p 0.05) in the vitamin C contents of A. vera and the combined plant materials, guava+A. vera. Guava had also significantly higher (p<0.05) DPPH scavenging ability (0.056 mg/ml), and TEAC (12.51±0.40 mM/gdw) than A. vera. The combined plant materials guava+ A. vera Original Research Article British Journal of Pharmaceutical Research, 4(3): 397-406, 2014 398 showed synergistic properties in the DPPH free radical scavenging ability (0.15 mg/ml) and antagonistic activity in the TEAC (4.58±0.17 mM/gdw). This study suggests that while guava may be a better antioxidant than A. vera when used separately, the combined plant materials produces synergistic antioxidant interaction, which could be used to enhance their medicinal applications.
Relative abundance of aquatic birds and their use of wetlands in the Patagonia of Southern Chile

The aquatic birds on 16 wetlands in Torres del Paine National Park, Chile, were surveyed monthly from July 1985 to June 1988. Seasonal patterns varied for each species, but average monthly numbers increased from 464 in August to a high of 911 in January, with migration from the park occurring from March to May. Average December density and diversity had significant negative linear regression relationships with pond or lake size. Absolute numbers were not related to size of wetland area. The Upland Goose (Chloephaga picta) and the Redgartered Coot (Fu/ica armil/ata) were the most abundant species and the ones found on the greates number of ponds and lakes.
Effectivity of Caspofungin on the Resistant Isolates of Candida albicans

Objective: To compare the performance of Caspofungin with the conventional antifungal drugs on the Candida albicans isolates.Materials and methods: Cases and Samples: The present study was carried out in the Department of Microbiology J. N. Medical College, AMU. Total of 6000 patients included in the study. Samples were collected according to their clinical presentation.Materials and methods: Cases and Samples: The present study was carried out in the Department of Microbiology J. N. Medical College, AMU. Total of 6000 patients included in the study. Samples were collected according to their clinical presentation.Evaluation and comparision of antifungal activity of Caspofungin: a. Disc diffusion, and b. Broth mico dilution method.Results: The susceptibility testing of Caspofungin and other conventional antifungal agents by broth dilution method revealed very low MIC of Caspofungin (0.062-1 μg/ml) as compared to fluconazole (1-64 μg/ml). Thus, Caspofungin proved to be more potent than other antifungals in vitro.Conclusion: We found Caspofungin to be more potent on the resistant isolates of Candida albicans in vitro.
The Effects of Feed Restriction on Reproductive Function in Sprague-Dawley Rats

Abstract The Effects of Feed Restriction on Reproductive Function in Sprague-Dawley Rats. Chapin, R. E. , Gulati, D. K. , Barnes, L. H. , and Teague, J. L. (1993). Fundam. Appl. Toxicol. 20, 23-29. Although it is known that severe feed restriction (FR) and weight reduction (to 50-60% of control values) will sharply impair rat reproduction, few data exist on the effects of less severe body weight reduction. In the present studies, adult Sprague-Dawley rats were feed restricted and maintained at 90, 80, and 70% of control body weight (CBW) for up to 17 weeks. In females, this treatment had no effect on fertility or total number of implants per dam, but transiently increased the length of the estrous cycle and decreased by 20% the number of corpora lutea in the 70% CBW females. Ovary weight at necropsy was decreased only in the 70% CBW group. Liver and kidney weights varied with body weight. In males, fertility was not affected when they were mated to nonrestricted females, While prostate and seminal vesicles weight varied with body weight, testis and epididymis weights were unchanged by any degree of FR, as were the number of sperm in the cauda epididymis and the number of homogenization-resistant spermatids in the testis. The percentage motile sperm was slightly decreased at all levels of FR. These data show that the Sprague-Dawley rat is largely resistant to adverse reproductive changes caused by feed restriction to 70% CBW. These data should be of use in interpreting changes seen in toxicity studies that produce weight-reduced Sprague-Dawley rats.
The Relation between Bacterial Toxic Action and Plant Growth Regulation

Coronatine is an extracellular toxin produced by Pseudomonas coronafaciens var, atropurpurea (REDDY and GODKIN), the incitant of the chocolate spot disease on Italian ryegrass (Lolium multiflorum LAM.). Coronatine induces the necrosis with chlorotic halo symptoms of the disease in host leaves as well as in nonhosts and thus is nonspecific. Coronatine, at low concentration of 0. 3μg to 30μg/ml, also induces marked hypertrophy only on potato tuber tissues. When a potato tuber cylinder was incubated with coronatine before the extensibility measurement, the plasticity was greater than that of the control. The results suggest that coronatine is required to increase the plasticity of the cell wall for cell expansion. These results indicate that coronatine may be characterized as a substance with high auxin-like activity. However, physiological data show that coronatine has a tissue specificity. It is clearly different from IAA. The difference in the tissue specificity strongly suggests that the two substances have different primary site of action. On the other hand, coronatine and IAA have fundamentaly the same final steps in increasing cell size. The steps involve some process which enhance the plasticity of the cell wall. Histological and physiological data show that the most sensitive site for coronatine injury may be in the cytoplasm. On the other hand, coronatine-induced hypertrophy of potato tuber tissues were markedly inhibited by N, N-dicyclohexylcarbodiimide, one of the inhibitors of membrane-bound ATPase. This results suggests a close association between coronatine and membrane-bound ATPase. In addition, coronatine changes the permeability 24 to 48 hours before the development of hypertrophy on potato tuber tissues. Thus, these results suggests that the disruption of the cytoplasm induced by coronatine may be due to secondaly effects brought about indirectly by physiological changes of the plasma membrane.
A conceptual model of solute acquisition by Alpine glacial meltwaters

Abstract Solute acquisition by Alpine glacial meltwaters is the result of the coupling of different pairs of reactions, one of which usually involves dissolved gases. Hence, the availability of atmospheric gases to solution is an important control on the composition of glacial meltwaters. The chemical compositions of the two main components of the bulk meltwater, quick flow and delayed flow, are dominated by different geochemical processes. Delayed flow waters are solute-rich and exhibit high p(CO2) characteristics. The slow transit of these waters through a distributed drainage system and the predominance of relatively rapid reactions, such as sulphide oxidation and carbonate dissolution, in this environment maximize solute acquisition. Quick-flow waters are dilute, both because of their rapid transit through ice-walled conduits and open channels, and because the weathering reactions are fuelled by relatively slow gaseous diffusion of (CO2) into solution, despite solute acquisition being dominated by rapid surface exchange reactions. As a consequence, quick flow usually bears a low or open-system p(CO2) signature. Bulk meltwaters are more likely to exhibit low p(CO2) values when suspended-sediment concentrations are high, which promotes post-mixing reactions. This conceptual model suggests that the composition of both quick flow and delayed flow is likely to be temporally variable, since kinetic, rather than equilibrium, factors determine the composition.
Crystal Structure of Tension Wood by X-ray Diffraction Method

Crystal structure of tension, opposite and lateral wood of Platanus orientalis L. were analysed in some aspects of crystallinity index, crystallite size, d-spacing of (200) and (004), and integrated intensity ratios with X-ray diffraction method. Crystallinity index and crystallite width in tension wood appeared somewhat larger than opposite or lateral wood. However, d-spacing and integrated intensity ratios were nearly identical irrespective of tension, opposite, and lateral wood.
Spatial organization of sodium calcium exchanger and caveolin-3 in developing mammalian ventricular cardiomyocytes

In adult cardiomyocytes, the established mechanism of excitation-contraction coupling is calcium-induced calcium release (CICR) mediated by L-type Ca channels (Cav1.2). Briefly, membrane depolarization opens voltage-gated Cav1.2 to allow for the influx of extracellular Ca into the cytosol. This small sarcolemmal (SL) Ca influx is necessary for triggering a larger release of Ca from the intracellular Ca storage site, the sarcoplasmic reticulum (SR), through the SR Ca release channel also known as the ryanodine receptor (RyR). RyR-mediated release of SR Ca effectively raises the cytosolic free Ca concentration, allowing for Ca binding to troponin C on the troponin-tropomysin complex, leading to cross-bridge formation and cell contraction. However, previous functional data suggests an additional CICR modality involving reverse mode Na-Ca exchanger (NCX) activity also exists in neonate cardiomyocytes. To further our understanding of how CICR changes occur during development, we investigated the spatial arrangement of caveolin-3 (cav-3), the principle structural protein of small membrane invaginations named caveolae, and NCX in developing rabbit ventricular myocytes. Using traditional as well as novel image processing and analysis techniques, both qualitative and quantitative findings firmly establish the highly robust and organized nature of NCX and cav-3 distributions during development. Specifically, our results show that NCX and cav-3 are distributed on the peripheral membrane as discrete clusters and are not highly colocalized throughout development. 3D distance analysis revealed that NCX and cav-3 clusters are organized with a distinct longitudinal and transverse periodicity of 1-1.5 μm and that NCX and cav-3 cluster have a pronounced tendency to be mutually exclusive on the cell periphery. Although these
Inoculation of Woody Legumes with Selected Arbuscular Mycorrhizal Fungi and Rhizobia To Recover Desertified Mediterranean Ecosystems

Revegetation strategies, either for reclamation or for rehabilitation, are being used to recover desertified ecosystems. Woody legumes are recognized as species that are useful for revegetation of water-deficient, low-nutrient environments because of their ability to form symbiotic associations with rhizobial bacteria and mycorrhizal fungi, which improve nutrient acquisition and help plants to become established and cope with stress situations. A range of woody legumes used in revegetation programs, particularly in Mediterranean regions, were assayed. These legumes included both exotic and native species and were used in a test of a desertified semiarid ecosystem in southeast Spain. Screening for the appropriate plant species-microsymbiont combinations was performed previously, and a simple procedure to produce plantlets with optimized mycorrhizal and nodulated status was developed. The results of a 4-year trial showed that (i) only the native shrub legumes were able to become established under the local environmental conditions (hence, a reclamation strategy is recommended) and (ii) biotechnological manipulation of the seedlings to be used for revegetation (by inoculation with selected rhizobia and mycorrhizal fungi) improved outplanting performance, plant survival, and biomass development.
Frequency of extended spectrum β-Lactamase producing Enterobacteriaceae in intensive care units

Background: The incidence of ESBL producing species have been steadily increased in recent years, resulting in limitation of infection control issues and therapeutic options.The purpose of this study was to evaluate prevalence of Enterobacteriaceae and also assess epidemiology ESBL producing strains isolated from patients admitted in ICUs. Methods: A total of one hundred fifty isolates were collected from urine, sputum, blood, wound and other clinical samples from patient admitted in ICU and then were identified by biochemical tests . All of the samples were screened by DAD method according to The NCCLS Guideline. The species that met NCCLS screening criteria was further tested for Clavulanic Acid effect by confirmatory method. Results: A total of one hundred fifty isolates,133(89.3%) were found to be resistant at least on of the indicators cephalosporin tested according to NCCLS Guideline. 121(80.6%) of the isolates were resistant to all the indicators tested . 89(59.3) isolateds were confirmed as ESBL producers. The number of isolates ESBL producing was as follow: Klebsiella pneumoniae 33 (76.74%), E.coli 20 (60.60%), Enterobacter cloacae 8 (47.05%), Citrobacter diversus 6 (54.54%), Enterobacter aerogenes 7 (53.84%), Citrobacter freundii 4 (40%), Klebsiella oxytoca 6 (62.5%), Proteus mirabilis 4 (50%), Serratia marcescens 2 (40%), Proteus Volgaris 0%. All of the isolates sensitive to imipenem. Conclusion: The present study shows high prevalence of ESBL producing Enterobacteriaceae from patients admitted in ICU . The increased rate of these species in most cases due to the administration of inadequate and irrational antimicrobial therapy . To overcome this problem, it needs to develop new antimicrobial agents, limiting the Unnecessary Use of antimicrobial and increasing compliance with infection control issues.
The demography of blackbirds Turdus merula in rural habitats : Is farmland a sub-optimal habitat?

1. A population of 54-76 pairs of European blackbirds Turdus merula L. encompassing woodland, farmland and edge (wood/farm boundary) habitats was studied to investigate whether farmland is a sub-optimal habitat for a bird species whose ancestral habitat was woodland. 2. Woodland was occupied at a higher density than farmland, but there was also variation within habitats: 'hot-spot' areas of woodland were occupied at high density, 'sparse' areas at low density, while other areas were completely unoccupied. 3. There was no effect of habitat or territory type on survival, although movements between years were more likely to be from farmland to woodland, and from sparse to hot-spot territories than vice versa. 4. There was a higher proportion of young males in farmland than in woodland, and in sparse territories compared to hot-spot territories; but there was no difference in the age structure of females across habitats. 5. Timing of breeding and clutch size did not differ between habitats or territory type, but farmland females laid smaller eggs than other females. 6. Reproductive success was low in all habitats (only 19% of attempts produced any fledglings), but there were significant differences between habitats in various measures of reproductive success. Success in farmland and woodland was similar, but hot-spot territories tended to be more successful than sparse territories. Predation was the major cause of failure. 7. Our results indicate that farmland blackbirds exhibit certain characteristics of populations in sub-optimal habitat, but the effect of habitat on reproductive success probably operated at a finer scale than this relatively crude habitat classification. 8. The distribution of breeding blackbirds is discussed in relation to ideal free and source-sink models.
CA-1, a Nove1 Phosphoprotein, lnteracts with the Promoter of the cabl40 Gene in Arabidopsis and 1s Undetectable in detl Mutant Seedlings

We have identified and partially purified a DNA binding protein from Arabidopsis that interacts specifically with the phytochrome-responsive promoter of the Arabidopsis cabl4O gene. Promoter deletion analyses in transgenic tobacco showed that, if a region that includes the sequence interacting with this protein was deleted, both expression and phytochrome responsiveness were lost. The protein protected a cytosine- and adenine-rlch region from DNase I digestion, and therefore it has been called CA-1. CA-1 was shown to be a phosphoprotein, and dephosphorylation changed the migration of the protein-DNA complex in DNA mobility shift assays. The data suggested that the protein has an apparent molecular weight of 70,000. The CA-1-protected region of the cabl40 promoter included an ACGT motif that has been found in the target sequences of a number of bZlP transcription factors, but the binding behavior of CA-1 differed from those factors. CA-1 binding activity was present in plants grown in either white light or darkness, and no differences in the binding activity were detected in the dark-grown plants after short red or white light treatments. However, the CA-1 binding activity was not detectable in extracts of seedlings bearing the detl mutation grown in the dark and given the same illumination treatments as wild type. In contrast to wild type, the mutant seedlings express cab RNA at a high leve1 when grown in complete darkness, and we found no further increase in cabl40 mRNA in response to brief red illumination. The lack of CA-1 activity in the detl mutant suggests that it may function as a transcriptional repressor regulating the expression of the cabl40 gene in Arabidopsis.
Survival of soybean rhizobia in summer and proliferation during monsoon in vertisols of Central India

High temperatures and desiccation of surface soil layers in semi-arid and arid tropical regions lead to severe decline in soil microbial populations and loss of microbial diversity. Low numbers of rhizobia in soil leads to poor nodulation and nitrogen fixation in legumes. The present study examined the impact of high temperatures of summer on the decline of microbial population in general and soybean rhizobia in particular, and the subsequent revival during monsoon season, at two Vertisol (Typic Eustochrept) sites in Central India, viz., Bhopal in M.P. and Durg in Chhattisgarh. Soil samples from 0 to 5, 5 to 15 and/or 15 to 30 cm depth from cultivated and fallow sites were sampled during peak summer, 2 and 45 or 95 days after onset of monsoon. Plate counts of bacteria, actinomycetes, and fungi revealed lower numbers in peak summer and also a general revival of microbes at both sites during the monsoon. The changes in microbial populations mirrored similar trends in soil biological (dehydrogenase) activity. The activity was lower at site-II due to lower soil organic matter content. Rhizobial numbers at both sites, determined by most probable number (MPN) method using plant infection assay with soybean as host, were lower than 100 cells per gram of soil in summer and while there was a small spurt after monsoon, they remained below the threshold (103g−1 soil) required for optimum nodulation. At site I, where the soils were continuously cultivated with soybean, the rhizobial population increased by 10 to 25 folds during monsoon whereas at site II under cultivation with a heterologous host (mungbean) it increased only by 3 to 8 fold. Decline of population of native rhizobia due to high temperature and low moisture and low populations even after monsoon, reinforced the need to regularly practice rhizobial inoculation of soybean each year to build up populations and to harness the optimum benefits of biological nitrogen fixation.