Miracle-dz/UniFAR-FTUs huggingface dataset:
Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin

Properties of macrophages and lymphocytes appearing in renal fibrosis remains to be investigated. F344 rats were injected once a week with cisplatin (2 mg/kg body weight) for 8 weeks and examined at post-final injection weeks 1, 3, 6, 9, and 12. Rats developed progressive renal fibrosis at weeks 1 to 6 as fibrosis-progress phase, and subsequent amelioration at weeks 9 and 12. CD68+ M1-macrophages and major histocompatibility complex (MHC) class II+ macrophages remarkably increased persistently, whereas CD163+ M2-macrophages slightly increased. MHC class II+/CD68+ and MHC class II+/CD163+ macrophages were present, indicating that MHC class II+ macrophages might have both functions of M1- and M2-macrophages. In the fibrosis-progress phase, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ for M1-factors, and transforming growth factor (TGF)-β1 and IL-10 for M2-factors tended to increase; tissue injury by M1 and fibrosis by M2 might have occurred simultaneously. Lots of CD4+ and CD8+ T cells appeared in close relation with MHC class II+ macrophages, and mainly CD4+ T cells formed aggregations. In the lymphocyte aggregates collected by laser microdissection, expression of IL-17A (for Th17 cells) and forkhead box P3 (FoxP3) (for Treg) significantly increased at weeks 1 and 6, respectively; presumably, Th17 cells might be involved in tissue injury, whereas Treg might be related to fibrosis amelioration. These results suggested that macrophages and T cells may contribute interrelatedly to renal fibrosis.
Practical value analysis of nursing risk management in intravenous drug allocation.

OBJECTIVE To explore the practical value of nursing risk management in intravenous drug allocation. METHODS A total of 1200 patients who received infusion therapy in our hospital were prospectively selected and divided into an observation group (n=600) and a control group (n=600) using a random number table method. The former was given nursing risk management, while the control group was not. The incidence rate of intravenous infusion errors, the incidence rate of infusion reactions, the scores of near-miss items in pharmacy intravenous admixture services and subjective satisfaction were observed and compared between the two groups. RESULTS The incidence of intravenous infusion errors and infusion reactions in the observation group were lower than those in the control group (P<0.05), while the scores of near-miss items in pharmacy intravenous admixture services and subjective satisfaction were higher than those in the control group (P<0.05). CONCLUSION Nursing risk management can reduce the incidence of errors in clinical pharmacy intravenous admixture services and the incidence rate of infusion reactions, improve infusion safety, and promote the improvement of the subjective satisfaction of patients.
Development and validation of nomogram for prediction of low birth weight: a large-scale cross-sectional study in northwest China

Abstract Background Birth weight is closely related to infant survival and future health, growth and development. In developing countries, the incidence of low birth weight is twice as high as in developed countries. Due to the low economic and medical level in northwest China, the problem of low birth weight needs to be solved urgently. Methods We developed the predictive model based on data sets from a cross-sectional study conducted in northwest China, and data were collected from August 2013 to November 2013. A total of 27,233 patients were included in the study. Least Absolute Shrinkage and Selection Operator (LASSO) regression was used to select the optimal predictive characteristics among risk factors. The selected characteristics in the LASSO regression were used in multivariate logistic regression to build the prediction model. C-index and calibration plot were used to evaluate the degree of discrimination and calibration of the model. The decision curve is used to evaluate the net benefit rate of the application of the predictive tool. Bootstrapping validation was used for internal validation. Results Nomogram included gestational age, the sex of the attendance, the mother's education level, antenatal care, the mother's occupation, pregnancy-induced hypertension, family income, exposure to pesticides and nutritional supplements. The C-index of the predicted nomogram was 0.698(95% confidence interval: 0.671–0.725), C-index of internal verification was 0.694, indicating that the model had a good identification ability. Calibration plot showed that the model had good calibration. Decision curve indicated that patients with a threshold probability of low birth weight between 1% and 71% would benefit more from using the prediction tool. Conclusion The use of this predictive model will contribute to clinicians and pregnant women to make personalized predictions easily and quickly so that early lifestyle detection and medical intervention can be undertaken by physicians and patients.
An empirical confirmation of diversified bet hedging as a survival strategy in unpredictably varying environments.

Environmental change jeopardizes the survival of species from variable environments by making the occurrence of favorable conditions less predictable. For organisms with long-lived propagules (e.g. spores, eggs or seeds), the theory of diversified bet hedging (DBH) predicts that delayed hatching over different growing seasons can help populations avoid extinction. Empirical observations in different organisms are consistent with DBH, but integrated tests that simultaneously validate the main theoretical assumptions and predictions are lacking. In this study, we combine field and multi-generational lab experiments to provide a complete test of DBH. Consistent with DBH predictions, resting egg clutches of the fairy shrimp Branchipodopsis wolfi, which inhabits rain-fed temporary rock pool environments with unpredictable inundations, hatched partially over a succession of inundations with identical hatching cues. Bet hedging was more common in populations from more unpredictable habitats where hatching fractions were lower. This differentiation in hatching strategies was preserved after two generations under common garden conditions, which implies intrinsic (epi-)genetic control of hatching. Finally, a demographic model confirmed that lower hatching fractions increase long-term population growth in unpredictable habitats. With this paper we propose a method to calculate probabilities of successful recruitment for organisms that use imperfect cues and show that this drives selection for variation in life history strategies as part of a DBH strategy.
Normative magnetic resonance imaging measurements of orbital structures in pediatric population of North-Eastern India: A retrospective cross-sectional study

Purpose: The people of Northeast India comprise mostly of Mongoloid descent with characteristic craniofacial features. The purpose of this study was to evaluate the normal orbital structures with MRI and determine normative data in the Northeast Indian pediatric population. Methods: MRI images of the 302 orbits of 151 pediatric patients below 16 years of age were retrospectively evaluated to measure the various orbital structures. Both axial and coronal MRI images were utilized for various orbital measurements. An independent sample t-test was done to compare various orbital data according to the sex and side. Linear regression was also done. Results: The mean age of the pediatric population was 9.64 ± 1.47 years with a male: Female ratio of 1.13:1. The mean interzygomatic line was 88.4 ± 9.09 mm and the interorbital line was 22.18 ± 3.62 mm. The mean thicknesses of medial, lateral, superior, and inferior recti muscles were 2.58 ± 0.46, 2.34 ± 0.42, 2.16 ± 0.40, and 2.53 ± 0.49 mm in males and 2.41 ± 0.41, 2.08 ± 0.34, 2.08 ± 0.46, and 2.46 ± 0.49 mm in females. The mean horizontal orbital, vertical orbital diameters, orbital index, mean volume of eyeball, and orbital cavity were 30.27 ± 2.97,3 7.06 ± 3.57, 122.58 ± 7.39, 4.63 ± 0.84, and 15.29 ± 3.52 in males, while 29.16 ± 3.23, 34.96 ± 3.99,119.96 ± 7.31, 4.49 ± 0.87, and 14.65 ± 3.47 in females, respectively. With an increase in age, the interzygomatic line (r = 0.883, r2 = 0.780; P < 0.0005), anterior medial interorbital line (r = 0.808, r2 = 0.652; P < 0.0005), mean volume of eyeball (r = 0.915, r2 = 0.838; P < 0.0005), orbital cavity (r = 0.924, r2 = 0.854; P < 0.0005), and distance between the optic nerve entry site (r = 0.829, r2 = 0.687; P < 0.0005) were increased. Conclusion: This study provides normative data of various orbital structures in a pediatric population and these data likely to be useful for diagnosing various pediatric orbital disorders and helps in the planning of various surgical procedures of orbits.
Extracorporeal carbon dioxide removal with the Advanced Organ Support system in critically ill COVID‐19 patients

Abstract Disturbed oxygenation is foremost the leading clinical presentation in COVID‐19 patients. However, a small proportion also develop carbon dioxide removal problems. The Advanced Organ Support (ADVOS) therapy (ADVITOS GmbH, Munich, Germany) uses a less invasive approach by combining extracorporeal CO2‐removal and multiple organ support for the liver and the kidneys in a single hemodialysis device. The aim of our study is to evaluate the ADVOS system as treatment option in‐COVID‐19 patients with multi‐organ failure and carbon dioxide removal problems. COVID‐19 patients suffering from severe respiratory insufficiency, receiving at least two treatments with the ADVOS multi system (ADVITOS GmbH, Munich, Germany), were eligible for study inclusion. Briefly, these included patients with acute kidney injury (AKI) according to KDIGO guidelines, and moderate or severe ARDS according to the Berlin definition, who were on invasive mechanical ventilation for more than 72 hours. In total, nine COVID‐19 patients (137 ADVOS treatment sessions with a median of 10 treatments per patient) with moderate to severe ARDS and carbon dioxide removal problems were analyzed. During the ADVOS treatments, a rapid correction of acid‐base balance and a continuous CO2 removal could be observed. We observed a median continuous CO2 removal of 49.2 mL/min (IQR: 26.9‐72.3 mL/min) with some treatments achieving up to 160 mL/min. The CO2 removal significantly correlated with blood flow (Pearson 0.421; P < .001), PaCO2 (0.341, P < .001) and HCO3‐ levels (0.568, P < .001) at the start of the treatment. The continuous treatment led to a significant reduction in PaCO2 from baseline to the last ADVOS treatment. In conclusion, it was feasible to remove CO2 using the ADVOS system in our cohort of COVID‐19 patients with acute respiratory distress syndrome and multiorgan failure. This efficient removal of CO2 was achieved at blood flows up to 300 mL/min using a conventional hemodialysis catheter and without a membrane lung or a gas phase.
Fertility preservation immediately after therapeutic abortion results in multiple normal follicular growth with the absence of mature oocytes due to early luteinization: a case report and literature review

Abstract Cancer therapy has priority over fertility preservation. The time available for fertility preservation in patients with cancer is often very limited and depends on the condition of the underlying disease. This case report presents the results of two rounds of controlled ovarian stimulations (COSs) performed after an induced abortion. The patient had mixed phenotype acute leukemia diagnosed during early pregnancy and underwent a surgical abortion, followed by ovarian stimulation using urinary follicle-stimulating hormone (uFSH) and gonadotropin-releasing hormone (GnRH) agonists. Oocyte retrieval was subsequently performed for oocyte cryopreservation. Despite good hormonal and ultrasonic follicular growth, no oocytes were obtained. During a second COS performed at a low human chorionic gonadotropin (hCG) level (less than 100 IU/L), several mature oocytes were obtained, suggesting that higher hCG levels during COS induce the absence of mature oocytes during normal follicular growth. It is recommended to start COS post-abortion after confirming a low hCG level while considering the timing of cancer treatment.
scAdapt: Virtual adversarial domain adaptation network for single cell RNA-seq data classification across platforms and species

Motivation In single cell analyses, cell types are conventionally identified based on known marker gene expressions. Such approaches are time-consuming and irreproducible. Therefore, many new supervised methods have been developed to identify cell types for target datasets using the rapid accumulation of public datasets. However, these approaches are sensitive to batch effects or biological variations since the data distributions are different in cross-platforms or species predictions. Results We developed scAdapt, a virtual adversarial domain adaptation network to transfer cell labels between datasets with batch effects. scAdapt used both the labeled source and unlabeled target data to train an enhanced classifier, and aligned the labeled source centroid and pseudo-labeled target centroid to generate a joint embedding. We demonstrate that scAdapt outperforms existing methods for classification in simulated, cross-platforms, cross-species, and spatial transcriptomic datasets. Further quantitative evaluations and visualizations for the aligned embeddings confirm the superiority in cell mixing and preserving discriminative cluster structure present in the original datasets. Availability https://github.com/zhoux85/scAdapt. Contact angyd25@mail.sysu. edu.cn or luojinx5@mail.sysu. edu.cn
Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa

Abstract Mammalian sperm carry a variety of highly condensed insoluble protein structures such as the perinuclear theca, the fibrous sheath and the outer dense fibers, which are essential to sperm function. We studied the role of cysteine rich secretory protein 2 (CRISP2); a known inducer of non-pathological protein amyloids, in pig sperm with a variety of techniques. CRISP2, which is synthesized during spermatogenesis, was localized by confocal immunofluorescent imaging in the tail and in the post-acrosomal region of the sperm head. High-resolution localization by immunogold labeling electron microscopy of ultrathin cryosections revealed that CRISP2 was present in the perinuclear theca and neck region of the sperm head, as well as in the outer dense fibers and the fibrous sheath of the sperm tail. Interestingly, we found that under native, non-reducing conditions CRISP2 formed oligomers both in the tail and the head but with different molecular weights and different biochemical properties. The tail oligomers were insensitive to reducing conditions but nearly complete dissociated into monomers under 8 M urea treatment, while the head 250 kDa CRISP2 positive oligomer completely dissociated into CRISP2 monomers under reducing conditions. The head specific dissociation of CRISP2 oligomer is likely a result of the reduction of various sulfhydryl groups in the cysteine rich domain of this protein. The sperm head CRISP2 shared typical solubilization characteristics with other perinuclear theca proteins as was shown with sequential detergent and salt treatments. Thus, CRISP2 is likely to participate in the formation of functional protein complexes in both the sperm tail and sperm head, but with differing oligomeric organization and biochemical properties. Future studies will be devoted to the understand the role of CRISP2 in sperm protein complexes formation and how this contributes to the fertilization processes. Summary sentence Biochemical properties of CRISP2 oligomers in boar spermatozoa.
Synergetic antimicrobial effect of chlorin e6 and hydrogen peroxide on multi-species biofilms

Abstract Antimicrobial photodynamic therapy (aPDT) has been considered as a potential alternative to antibiotics for the treatment of biofilm infections. There is evidence that an additional H2O2 enhances the antimicrobial efficacy of aPDT. However, the minimum H2O2 concentration to achieve this synergistic effect is unclear. A saliva-derived multi-species biofilm was treated with the photosensitizer chlorin e6 (Ce6, 50 µM), H2O2 (0.3, 3.3, 33.3 mM), or their combination for 5 min, followed by no irradiation or irradiation at 15 J (cm2)−1 (λ = 450 nm or 660 nm), with or without oxygen. Biofilm viability and metabolic activity were evaluated. The combination of 33.3 mM H2O2 and Ce6-aPDT strongly enhanced antimicrobial efficacy compared with either component alone, irrespective of oxygen availability and irradiation wavelength. In particular, the combination resulted in a 6.6-log colony forming unit (CFU) reduction anaerobically under blue irradiation. This combination is a promising treatment for biofilm infections, especially those thriving in an anaerobic microenvironment.
Does robotic assisted technology improve the accuracy of acetabular component positioning in patients with DDH?

Background: Accurate positioning of the acetabular component is key in performing total hip arthroplasty (THA). However, reconstruction of the acetabulum in the setting of developmental dysplasia of the hip (DDH) is a challenge. Robotic assisted THA has the potential to improve the accuracy of implantation of the acetabular cup in cases with DDH. The purpose of this study was to assess whether robotic technology improves the accuracy of acetabular component positioning in patients with DDH. Material and methods: We included 59 THAs using robotic assisted technology from June 2019 to January 2020 as the study group. These were compared to conventional THAs without robotic technology after control for age, gender, body mass index (BMI), Crowe type and operation date. Radiographic measurements were taken by 2 blinded orthopaedic residents. The percentage of hips within the Lewinnek and Collanan safe zones were calculated, along with acetabular rotation centers for the “target zone. ” Surgical time and perioperative bleeding were also compared between both groups. Results: One patient suffered dislocation in conventional group while no dislocation occurred in robotic group. The acetabular components of the robotic assisted group had more cases located within the Lewinnek (p = 0.013) and Collanan (p = 0.008) safe zones than conventional group (94.9% vs 79.7% and 74.6% vs 50.8%). There were 7 cases in conventional group and 4 cases in robotic group that had more lateral or more superior rotational centers of THA, but did not reach statistical significance (p = 0.342). No statistical difference was detected between groups with regards to blood loss (p = 0.098) and surgical time (p = 0.602). Conclusion: Robot assisted technology can assist surgeons with implanting acetabular cups more in Lewinnek and Callanan safe zone than conventional techniques without additional blood loss and surgical time. Level of Evidence: Therapeutic Level Ⅲ.
Neonatal mortality rates, characteristics, and risk factors for neonatal deaths in Ghana: analyses of data from two health and demographic surveillance systems

ABSTRACT Background Reducing neonatal mortality rates (NMR) in developing countries is a key global health goal, but weak registration systems in the region stifle public health efforts. Objective To calculate NMRs, investigate modifiable risk factors, and explore neonatal deaths by place of birth and death, and cause of death in two administrative areas in Ghana. Methods Data on livebirths were extracted from the health and demographic surveillance systems in Navrongo (2004–2012) and Kintampo (2005–2010). Cause of death was determined from neonatal verbal autopsy forms. Univariable and multivariable logistic regression were used to analyse factors associated with neonatal death. Multiple imputations were used to address missing data. Results The overall NMR was 18.8 in Navrongo (17,016 live births, 320 deaths) and 12.5 in Kintampo (11,207 live births, 140 deaths). The annual NMR declined in both areas. 54.7% of the births occurred in health facilities. 70.9% of deaths occurred in the first week. The main causes of death were infection (NMR 4.3), asphyxia (NMR 3.7) and prematurity (NMR 2.2). The risk of death was higher among hospital births than home births: Navrongo (adjusted OR 1.14, 95% CI: 1.03–1.25, p = 0.01); Kintampo (adjusted OR 1.76, 95% CI: 1.55–2.00, p < 0.01). However, a majority of deaths occurred at home (Navrongo 61.3%; Kintampo 50.7%). Among hospital births dying in hospital, the leading cause of death was asphyxia; among hospital and home births dying at home, it was infection. Conclusion The NMR in these two areas of Ghana reduced over time. Preventing deaths by asphyxia and infection should be prioritised, centred respectively on improving post-delivery care in health facilities and subsequent post-natal care at home.
TNF-α inhibitor-induced paradoxical psoriasis and erythema nodosum in a patient with ankylosing spondylitis

EJD, vol. 31, n◦ 3, May-June 2021 undergoing PD-1/PD-L1 inhibitor therapy are reported to develop BP [7]. However, the present case is only the second reported DP case caused by nivolumab or another ICI [8]. In contrast to our case, the first reported case was anti-BP180-NC16A antibody-negative and antiLAD-1 IgG antibody-positive [8]. Although ICI-induced BP (ICI-BP) was reported to pose a potentially greater likelihood of prolonged intractable pruritus than classic idiopathic BP [6], the present patient did not report pruritus. As the patient was not receiving any medications known to induce BP, the possibility of other drug-induced pemphigoid was ruled out. Histopathologically, individual cell keratinization was recognized in the lesional epidermis, however, this is not a feature of BP/DP, including classic drug-induced BP and ICI-BP, and therefore might be unique to ICI-DP. We encountered DP as an extremely rare side effect of nivolumab. When palmar and plantar erythema emerge after nivolumab administration, the possibility of DP should be considered and biopsies and serological testing should be performed early.
Hyperglycaemia-associated Caspase-3 predicts diabetes and coronary artery disease events

Apoptosis is central in both diabetes and atherosclerosis, linked to pancreatic beta cell death and plaque progression. Circulating Caspase‐3 has also been associated with diabetes and coronary calcium score. Here, we explored if soluble Caspase‐3 (sCaspase‐3) is associated with cardio‐metabolic risk factors and predicts incidence of diabetes and coronary artery disease (CAD).