Miracle-dz/UniFAR-FTUs huggingface dataset:
Ellagic acid induces esophageal squamous cell carcinoma cell apoptosis by modulating SHP‐1/STAT3 signaling

Ellagic acid (EA) has been reported to have antiproliferative and antioxidant properties, but its function in esophageal squamous cell carcinoma (ESCC) has not been investigated yet. In the current study, EA was found have a significant anti‐tumor activity in ESCC. In specific, EA inhibited ESCC cell survival in both of a concentration‐ and time‐dependent manner. And our results showed that EA promoted ESCC cell apoptosis, including inducing the cleavages of PARP, and inhibiting the expression of anti‐apoptotic proteins. In mechanistic, EA markedly suppressed STAT3‐driven luciferase activity, and inhibited both of the endogenous and cytokines‐induced STAT3 activation in ESCC cells. Further investigations indicated that EA could significantly upregulate SHP‐1 expression, a negative modulator of STAT3 signaling. In contrast, knockdown of SHP‐1 could attenuate the effects of EA on inhibiting ESCC cell survival. Moreover, we found that EA could inhibit RNF6 expression, an E3 of SHP‐1, and overexpressing RNF6 could also significantly attenuate the effects of EA on inhibiting ESCC cell survival, which further revealed that EA could inhibit STAT3 signaling by modulating RNF6/SHP‐1 axis. Our present study indicated that EA could be as a novel STAT3 inhibitor for the treatment of ESCC.
The time is now: expedited HIV differentiated service delivery during the COVID‐19 pandemic

At its core, differentiated service delivery (DSD) for HIV is centred around clients’ needs and expectations and relieving unnecessary burdens on the health system [1]. In the 2016 World Health Organization (WHO) antiretroviral therapy (ART) guidelines, it was acknowledged that adaptations to the delivery of HIV services were necessary to achieve the “treat all” recommendation [2]. This transition from a “onesize-fits-all approach” to DSD means modifying the location, frequency and package of services as well as the cadre providing services, considering the clinical needs, specific population and the context including urbanicity, stability of context (for example high migration, conflict or pandemic) and type of HIV epidemic [2-4]. Existing global and national policies around DSD for HIV can be leveraged during the COVID-19 pandemic to play a critical role in supporting uninterrupted ART and reducing avoidable contact with health facilities, thereby supporting health systems to focus on COVID-19. Recent statements from The Global Fund for HIV, Tuberculosis and Malaria (Global Fund), the Global Network of People Living with HIV (GNP+), UNAIDS, the United States President’s Emergency Plan for AIDS Relief (PEPFAR) and the WHO all endorse leveraging components of DSD for people living with HIV (PLHIV) during the COVID-19 pandemic [4-9]. The time is now to accelerate access to DSD for all PLHIV. We acknowledge that accelerating access requires capacity in addition to policy decisions. Scaling up the provision of longer ART refills is highly dependent on supply chains that may be currently under threat. There are concerns of constraints in ART production in the coming months as a result of the lockdown in India; with the Global Fund currently rating the operational risk assessment as “moderate” [10]. The global supply situation is being closely monitored and coordinated by partners with PEPFAR tempering language around the duration of multi-month dispensing to ensure continuity of care [11,12]. Countries are being encouraged to submit orders well in advance, adjust supply plans for longer leads times, distribute stock to clinics rather than holding it centrally and transparently communicate stock levels by regimen at the national and provincial level to support planning [11,13,14]. Concerns regarding ritonavir-boosted lopinavir availability pre-dated COVID-19, and plans to transition to other antiretrovirals are being accelerated in countries with large numbers of patients on this regimen [15]. With many sub-Saharan countries’ forecasts increasing numbers of PLHIV on a Dolutegravir (DTG)-based first-line regimen and a slower enrolment and transition to DTG regimens than predicted, this stock may be less threatened than the Efavirenz (EFV)-based regimens. This further strengthens the need for supporting patients to immediately transition to DTG regimens without requiring additional clinical monitoring visits to health facilities.
Application of nanomaterials in treatment, anti-infection and detection of coronaviruses

Nanotechnology and nanomedicine have excellent potential in dealing with a range of different health problems, including viruses, which are considered to be a serious challenge in the medical field. Application of nanobiotechnology could represent a new avenue for the treatment or disinfection of viruses. There is increasing concern regarding the control of coronaviruses, among these, Middle East respiratory syndrome coronavirus, severe acute respiratory syndrome coronavirus and severe acute respiratory syndrome coronavirus-2 are well known and dangerous examples. This article aims to provide an overview of recent studies on the effectiveness of nanoparticles as diagnostic or antiviral tools against coronaviruses. The possibilities of effectively using nanomaterials as vaccines and nanosensors in this field are also presented.
Auditory cortical alpha/beta desynchronization prioritizes the representation of memory items during a retention period

To-be-memorized information in working-memory could be protected against distracting influences by processes of functional inhibition or prioritization. Modulations of oscillations in the alpha to beta range in task-relevant sensory regions have been suggested to play an important role for both mechanisms. We adapted a Sternberg task variant to the auditory modality, with a strong or a weak distracting sound presented at a predictable time during the retention period. Using a time-generalized decoding approach, relatively decreased strength of memorized information was found prior to strong distractors, paralleled by decreased pre-distractor alpha/beta power in the left superior temporal gyrus (lSTG). Over the entire group, reduced beta power in lSTG was associated with relatively increased strength of memorized information. The extent of alpha power modulations within participants was negatively correlated with strength of memorized information. Overall, our results are compatible with a prioritization account, but point to nuanced differences between alpha and beta oscillations.
Effect of loading practices and 6-hour road transport on the physiological responses of yearling cattle

A controlled study using 16 yearling Angus steers was conducted to determine the physiological responses associated with loading practices followed by 6 h of road transport and 17 h of post-transport recovery. The cattle were quietly mustered from grazing at pasture and directly loaded onto a truck for transport. During loading, cattle received either four consecutive prods with a commercial electric prodder (n = 8) or no prodding (control, n = 8). The experiment was performed in four replicates, conducted on consecutive days, with four animals (n = 2 per treatment) utilised on each day. On the truck, cattle were confined to pens that each held a single animal facing the direction of travel. Blood samples were taken via jugular catheters before and during the 6 h journey and during the 17 h recovery phase. Samples were analysed for haematology, osmolality and plasma cortisol, total protein, creatine kinase, blood urea nitrogen and the acute phase protein haptoglobin. The physiological measurements indicated that most stress occurred during loading and the initial stages of transport, but after this, the cattle habituated and were able to cope with the 6 h of transport. After 17 h of recovery, nearly all the variables measured had returned to their pre-transport levels. Use of an electric prodder during loading did not modify the physiological responses to loading, transport or the rate of recovery compared with the controls.
CircRNA WHSC1 targets the miR‐646/NPM1 pathway to promote the development of endometrial cancer

Circular RNAs (circRNAs) play important roles in human cancer progression. Their high stability and tissue specificity make circRNAs important molecular targets for clinical diagnosis, treatment and prognosis. However, the functions and molecular mechanisms of circRNA WHSC1 in endometrial cancer are unknown. CircWHSC1 expression in normal endometrial and endometrial cancer tissues was detected using PCR. Overexpression or knockdown of circWHSC1 in endometrial cancer cell lines HEC‐1B or Ishikawa, respectively, cell function experiments were used to detect the impact of circWHSC1 on endometrial cancer cells. A nude mouse xenograft model was used to detect changes in tumorigenesis of HEC‐1B cells after circWHSC1 overexpression. Bioinformatics and dual luciferase reporter gene technology were used to predict and validate the sponging ability of circWHSC1 on microRNAs. Gene expression changes were detected by using Western blotting. CircWHSC1 expression was increased in endometrial cancer tissues. CircWHSC1 overexpression promoted the proliferation, migration and invasion of endometrial cancer cells and decreased apoptosis. CircWHSC1 knockdown had the opposite effect. CircWHSC1 overexpressed nude mice showed increased tumorigenicity. Bioinformatics predicted that circWHSC1 binds to miR‐646, which was confirmed using luciferase reporter gene assays. High expression of miR‐646 could reverse the effect of circWHSC1 on endometrial cancer cells. Western blotting showed increased or decreased levels of nucleophosmin 1 (NPM1), an miR‐646 downstream target, after circWHSC1 overexpression or knockdown, respectively. CircWHSC1 promotes endometrial cancer development through sponging miR‐646 and targeting NPM1.
Influence of microalgae addition in formulation on colour, texture, and extrusion parameters of corn snacks

Extrusion is a more and more popular technique for snack production using interesting raw ingredients, to produce snacks improving their nutritional value. The aim of this study was to obtain corn extrudates enriched with different concentration levels of Arthrospira platensis, Chlorella vulgaris, and Nannochloropsis gaditana biomass and to compare their expansion parameters and physicochemical properties with control corn extrudates. Expansion, physicochemical, and compositional parameters were analysed. Microalgae addition to formulations gave extruded snacks and provoked no major changes compared to control corn extrudate. Addition of microalgae in extrudates formulation implies a slight reduction of water absorption index, swelling index, bulk density, and hygroscopicity and a slight increase in water solubility index, expansion index, and porosity. Noticeable changes for the addition of microalgae changed the extruded snack from translucent to opaque and total colour difference values of were perceptible by human eye.
Model of Pathological Collagen Mineralization Based on Spine Ligament Calcification

The aim of the study was to determine the time of mineral growth in human spine ligaments using a mathematical model. The study was based on our previous research in which the physicochemical analysis and computed microtomography measurements of deposits in ligamenta flava were performed. Hydroxyapatite-like mineral (HAP) constituted the mineral phase in ligament samples, in two samples calcium pyrophosphate dehydrate (CPPD) was confirmed. The micro-damage of collagen fibrils in the soft tissue is the crystallization center. The growth of the mineral nucleus is a result of the calcium ions deposition on the nucleus surface. Considering the calcium ions, the main component of HAP, it is possible to describe the grain growth using a diffusion model. The model calculations showed that the growth time of CPPD grains was ca. a month to 6 years, and for HAP grains >4 years for the young and >5.5 years for the elderly patients. The growth time of minerals with a radius >400 μm was relatively short and impossible to identify by medical imaging techniques. The change of growth rate was the largest for HAP deposits. The mineral growth time can provide valuable information for understanding the calcification mechanism, may be helpful in future experiments, as well as useful in estimating the time of calcification appearance.
Adaptation of Medicago rigidula to a cereal–pasture rotation in north-west Syria

SUMMARY The ley-farming system (integrated cereal and livestock production in which cerealsrotate with self-regenerating pastures) is considered to be of great potential benefit to north Africa and west Asia. In the colder parts of this region (of which north Syria is typical) its application is limited by poor adaptation of commercial medic cultivars (mainly Medicago truncatula and M. littoralis). An extended selection programme hag identified M. rigidula as adapted to the soils and climate of the region but nothing is known of its adaptation to the ley-farming system itself. An experiment which included 23 selections of M. rigidula and one each of M. rotata and M. noeana was conducted over 3 years during which herbage production, seed yield, and the fate of seeds were observed during the 1st year when pastures were established, the 2nd year when wheat was sown, and the 3rd year when the pasture regenerated. Of the 400–800 kg seed/ha produced in the 1st year an average of 87% remained in the soil in spring of the 3rd year. The weight of seed regenerating in the 3rd year varied from 30 to nearly 170 kg/ha, and herbage production, especially in winter, depended heavily on the number of regenerating seedlings. The most productive regenerating pastures produced nearly 2 t/ha of dried herbage by 1 January, and more than 6 t/ha for the whole growing season. The results showed that there was sufficient residual and newly produced seed at the end of the 3rd year to be sure that subsequent regeneration would result in similar herbage yields in the 5th year, and that the pasture was assured of long-term persistence. The significance of this for livestock production is discussed, and it is concluded that the results should encourage further investigation of grazing management and socioeconomic factors seen as constraints to introducing the ley-farming system to north Syria.
No-Tillage Corn Hybrids Response to Starter Fertilizer

Starter fertilizer can help improve early-season corn (Zea mays L.) growth, especially under cool soil conditions, but corn hybrids may differ in their response to starter fertilizer. This study evaluated the response of corn hybrids to complete starter fertilizers applied under no-tillage, dryland conditions. Nine site-years of data were collected from 1993 through 1995 at four geographical locations: Doon (NW1) in extreme NW Iowa, Sutherland (NW2) in central NW Iowa, Lewis (SW), and Crawfordsville (SE). The soil types were as follows: NW1-Moody silty clay loam (fine-silty, mixed, superactive, mesic Udic Haplustolls); SW-Marshall silty clay loam (fine-silty, mixed, superactive, mesic Typic Hapludolls); SE-Nira silty clay loam (fine-silty, mixed, superactive, mesic Oxyaquic Hapludolls); and NW2-Primghar silty clay loam (fine-silty, mixed, superactive, mesic Aquic Hapludolls). Each hybrid was planted with and without starter fertilizer applied 2 in, to the side and 2 in, below the seed at planting. Hybrid-by-starter interactions were not significant for early-season dry matter production, nutrient concentration, or grain yield in any of the site-years, indicating that the hybrids, on average, responded similarly to starter fertilizer. Starter fertilizer increased early-season growth significantly in four of eight site-years and grain yield in seven of nine site-years. Average grain yield increases ranged from 4 bu/acre at the NW2 site in 1993 to 18 bu/acre at the SE site in 1995. Yield increases were not significant either year at the NW1 site. Increases in dry matter yield ranged from 18.7% at the SW site to 97.8% at the SE site in 1994. Starter fertilizer affected tissue K concentration to a much larger degree than it did N and P concentrations especially at the SE site. The results suggest that starter fertilizer will likely benefit no-tillage corn for most hybrids in the northern corn belt even on soils with adequate P and K.
Cyanobacterial carbonic anhydrases

Carbonic anhydrases (CAs) are ubiquitous zinc metalloenzymes that catalyze the reversible dehydration of HCO3. These enzymes are encoded by at least five distinct, evolutionarily unrelated gene families, four of which have been found among the cyanobacteria examined to date. However, the distribution and expression of these cyanobacterial α-, β-, γ-, and ∈-CAs and their homologues among species have not yet been investigated in great detail. In this study, the number, distribution, and catalytic function of known and putative CAs and CA-like proteins from a variety of freshwater and marine cyanobacteria are examined.Key words: carbonic anhydrase, carboxysome, CO2-concentrating mechanism, cyanobacteria, Prochlorococcus, Synechococcus, Synechocystis.
Considerations on the diet composition and feeding rate of dermersal sharks in 15 European public aquaria

To get an overview of the feeding practices applied to dermersal sharks in captivity, a questionnaire was sent to 15 European public aquaria. The most common dermersal shark species kept in the participating aquaria are Carcharhinus plumbeus (n=9), Carcharias taurus (n=7), and C. melanopterus (n=6). Body weight (BW) was calculated using captive estimated body length (BL) and actual weight data obtained from commercial fisheries. The estimated BW for C. plumbeus in all participating aquaria is higher than the calculated BW. The feeding rate (FR) for adult C. plumbeus varied widely within participating aquaria (2.3–11.5% BWcal/week or 32–112 kcal/kg BWcal/week). The FR for adult C. plumbeus was lower (1.2–7.9% BWest/week or 17–99 kcal/kg BWest/week) using the estimated BW. Adult Carcharias taurus were fed at 1.3–5.0% BWcal/week or 70–170 kcal/kg BWcal/week. Negaprion brevirostris was fed at 5.0% BWest/week or 57 kcal/BWest/week. The relationship between FR and estimated BW is expressed as FR=54.5 e−0.033BW. In this study, the feeding frequency for C. plumbeus varied between 1–7 times a week, with an average of 3.3 times a week. Diet in all aquaria consisted of both lean and fatty fish, and sometimes squid or crustaceans. Supplementation of vitamins is common practice in 80% of the surveyed aquaria, although a wide variety of quantity and quality was observed. Ten aquaria (66%) add iodine, as potassium iodide (0.4–12.5 mg KI/kg BW/week) or calcium-iodate (322–1,500 mg/kg food/week), to their food. Zoo Biol 22:203–226, 2003. © 2003 Wiley-Liss, Inc.
AFB1 Induced Transcriptional Regulation Related to Apoptosis and Lipid Metabolism in Liver of Chicken

Aflatoxin B1 (AFB1) leads to a major risk to poultry and its residues in meat products can also pose serious threat to human health. In this study, after feeding 165-day-old Roman laying hens for 35 days, the toxic effects of aflatoxin B1 at different concentrations were evaluated. The purpose of this study was to explore the mechanism of liver toxicosis responses to AFB1. We found that highly toxic group exposure resulted in liver fat deposition, increased interstitial space, and hepatocyte apoptosis in laying hens. Furthermore, a total of 164 differentially expressed lnRNAs and 186 differentially expressed genes were found to be highly correlated (Pearson Correlation Coefficient > 0.80, p-value < 0.05) by sequencing the transcriptome of control (CB) and highly toxic group (TB3) chickens. We also identify 29 differentially expressed genes and 19 miRNAs that have targeted regulatory relationships. Based on the liver cell apoptosis and fatty liver syndrome that this research focused on, we found that the highly toxic AFB1 led to dysregulation of the expression of PPARG and BCL6. They are cis-regulated by TU10057 and TU45776, respectively. PPARG was the target gene of gga-miR-301a-3p, gga-miR-301b-3p, and BCL6 was the target gene of gga-miR-190a-3p. In summary, highly toxic AFB1 affects the expression levels of protein-coding genes and miRNAs in the liver of Roman layer hens, as well as the expression level of long non-coding RNA in the liver, which upregulates the expression of PPARG and downregulates the expression of Bcl-6. Our study provides information on possible genetic regulatory networks in AFB1-induced hepatic fat deposition and hepatocyte apoptosis.
Human needs in COVID-19 isolation

To reduce the spread of COVID-19, the World Health Organization and the majority of governments have recommended that the entire human population should ‘stay-at-home’. A significant proportion of the population live alone or are vulnerable to mental health problems yet, in the vast majority of cases, individuals in social isolation have no access to mental healthcare. The only resource is people themselves using self-help, self-medication and self-care. During prolonged COVID-19 isolation, an in-built system of homeostasis can help rebalance activity, thought and feeling. Increased physical activity enables a reset of physical and mental well-being. During periods of lockdown, it is recommended that exercise should be as vigorously promoted as social distancing itself.
Leafing phenology and timing of egg laying in great tits Parus major and blue tits P-caeruleus

To evaluate the importance of tree leafing for the start of laying and clutch size of birds, we compared the breeding phenology of great tits Parus major and blue tits P. caeruleus between one coastal and two inland sites in the same geographical region. Because of the cooling influence of the sea, trees at the coastal site were known to initiate budburst about a week later than at the inland sites. During 5 years, breeding by the tits and the leaf phenology of birch Betula pendula, and oak Quercus robur were monitored. The leaf phenology of birch and oak explained a significant part of the between-year variation in the start of egg laying in blue and great tits, respectively. The tits started laying earlier at the sites with an early budburst, i.e. normally inland. However, leaf phenology was not an absolute cue to the start of laying, since blue tits laid earlier relative to leafing at the inland site than at the coastal site, and both tit species laid eggs earlier relative to leafing during late springs. In neither species was clutch size affected by leafing phenology. However, great tit females at the coastal site consistently produced fewer eggs than did those at the inland site. No such difference was found in the blue tits. Although leafing phenology may predict the start of laying in tits, other factors also influence its timing. These factors might include other cues, or differing life-history trade-offs depending on site or general climatic factors during the spring.
Towards effective COVID-19 vaccines: Updates, perspectives and challenges (Review)

In the current context of the pandemic triggered by SARS-COV-2, the immunization of the population through vaccination is recognized as a public health priority. In the case of SARS-COV-2, the genetic sequencing was done quickly, in one month. Since then, worldwide research has focused on obtaining a vaccine. This has a major economic impact because new technological platforms and advanced genetic engineering procedures are required to obtain a COVID-19 vaccine. The most difficult scientific challenge for this future vaccine obtained in the laboratory is the proof of clinical safety and efficacy. The biggest challenge of manufacturing is the construction and validation of production platforms capable of making the vaccine on a large scale.