Miracle-dz/UniFAR-FTUs huggingface dataset:
Epidemiological study of injuries caused by violence and conflict in forensic medical records of selected cities of Sistan and Baluchistan province in 2020

Background and Aims: Interpersonal violence is a socially traumatic and unpleasant phenomenon. These violence-related injuries are sometimes irreparable and can become a permanent problem, Violence in Iran is one of the five most socially harmed and has increased dramatically in recent years, therefore the present research aimed to investigate epidemiological study of injuries caused by violence and conflict in forensic medical records of selected cities of Sistan and Baluchistan province in 2020 considering this subject must be one of our priorities. Methods: This study was a descriptive study performed in forensic medicine centers of two cities in Sistan and Baluchistan province, Iran. The population consisted of existing citations and records, sample size based on similar studies and the recommendation of professors, all available records were considered and census method was used to select the samples. The data gathering tool consisted of two sections: demographic information and violence and conflict. Content validity method and reliability test were used to determine the validity and reliability. Data were analyzed using SPSS24 software. Results: The results showed that the majority of the sample was 20-29 years old, male, married, self-employed and high school graduated, living in the eastern part of the city, with no history of mental illness, domestic violence, child abuse, and spouse abuse. The majority of the study units did not use any drugs. The most violent were beatings and bullying. It included several organs such as limbs, soft tissue damage with bleeding. Conclusion: The results of this study indicate the importance of epidemiological analysis of violence and conflict in the provinces under study and show that appropriate solutions and culture building, as well as increasing awareness of the impact of violence and conflict on individuals and their injuries.
A Report of an Unusual Case of Pediatric Eosinophilia Associated with Platelet-Derived Growth Factor Receptor-Beta Rearrangement

Abstract Eosinophilia is a common finding in the pediatric age group. While the majority of mild eosinophilia cases are benign and due to reactive causes, persistent hypereosinophilia is uncommon and requires prompt clinical evaluation because of the potential risk of end-organ damage associated with it. Given the broad differential diagnoses of eosinophilia, it is essential to have a systematic approach to the evaluation of unexplained eosinophilia in children. Here, we discuss the case of a 2-year-old child who presented with very high eosinophil counts. A systematic workup of the case helped us in arriving at a rare diagnosis of platelet-derived growth factor receptor-beta (PDGFRB)-rearranged clonal eosinophilia. Identification of such an entity is important as it has therapeutic implications, and early recognition helps in preventing associated end-organ damage by instituting appropriate therapy. Such cases of eosinophilia associated with platelet-derived growth factor receptor-alpha and PDGFRB rearrangement respond dramatically to imatinib.
Correlation of color Doppler ultrasound and pathological grading in endometrial carcinoma

Introduction: Given the role of angiogenesis in tumor growth, the evaluation of tissue vascularization by Doppler ultrasound has been thought to be useful in the prediction of malignant endometrial changes. The aim of this study was to evaluate the efficacy of transvaginal color Doppler ultrasound (TV-CDU) findings in the differentiation between endometrial hyperplasia and endometrial carcinoma and its relation with pathologic findings. Methods: This observational study included 48 women with either endometrial hyperplasia (n = 10) or endometrial carcinoma (n = 38) that had been diagnosed by endometrial biopsy. The intratumoral blood flow characteristics including resistance (RI), pulsatility (PI) and peak systolic velocity (PSV) index were analyzed using TV-CDU before surgery. Endometrial thickness and myometrial invasion also was assessed in all patients using gray-scale ultrasound. Then the relationship between these ultrasound findings and histologic results was evaluated with EC. Results: RI, PI, and PSV indices in endometrial carcinoma were significantly higher than endometrial hyperplasia (P < 0.0001). There was also a significant difference between the mean endometrial thickness between the two groups of endometrial hyperplasia and endometrial carcinoma (P < 0.0001). Intratumoral blood flow index were higher in high grade tumors than in low grade tumors (P < 0.05). Conclusion: TV-CDU may be useful to show a difference the difference in tumor angiogenesis between endometrial hyperplasia and endometrial carcinoma and therefore be used in differentiation of endometrial hyperplasia and carcinoma. Evaluation of intratumoral blood flow using RI, PI, and PSV indices in patients with endometrial carcinoma may be helpful distinguishing between low-grade and high-grade tumors as well as preoperative tumor invasion before surgery.
The Red-fin Goby, Evorthodus minutus Meek & Hildebrand, 1928: a new record from Santa Cruz Island, Galapagos Archipelago

The Red-fin Goby, Evorthodus minutus Meek & Hildebrand, 1928, is a coastal brackish species which is commonly distributed from Sinaloa, Mexico to Guayaquil, Ecuador and particularly abundant in mangroves of Central American eastern Pacific. We report a new record of E. minutus collected from the Santa Cruz Island, part of the Galapagos Archipelago. This new record represents a range extension and is allows for a relevant discussion about colonization pathways in the equatorial eastern Pacific of a brackish-water species.
Phobia among residents in board training programs in Abha, Kingdom of Saudi Arabia, 2019

Background: Phobia is an extreme form of anxiety or fear which is triggered by a given situation or object or even danger associated with a situation or object for more than 6 months. There are three main forms of phobia: social phobia (social anxiety disorder), specific phobia, or agoraphobia. Agoraphobia is a form of anxiety disorder where one fears and avoids situations or places that might cause them to panic. The main purpose of this study is to investigate phobia among residents in broad training programs in Abha city and compare phobia between residents in broad training programs and general practitioners in Abha city. Methods: It is a cross-sectional study. The study was conducted in Abha city of Saudi Arabia from December 2019 to March 2020. It included a representative sample of medical doctors under broad training programs and general practitioners (none trained) in all the health-care centers. A link for the survey questionnaires was created and shared with the respondents. The questionnaire included sociodemographics of the participants, the Kutcher Generalized Social Anxiety Disorder Scale, and the Psychometric Properties and Clinical Utility of the Specific Phobia Questionnaire in an Anxiety Disorders Sample for specific phobia. Results: The study included 300 physicians. Majority of them (81%) were in the age group between 25 and 30 years. Males represent 54% of them. There was no statistically significant difference between resident physicians and general practitioners regarding the avoidance of different social situations. Conclusion: Overall, the residents in board training programs in Abha city expressed lower levels of discomfort, anxiety, distress, avoidance, fear, and life interference of some social and specific situations compared to general practitioners.
COVID-19: A Review of the Pandemic with Emphasis on the Role of Imaging

Abstract Heretofore, the COVID-19 pandemic continues to expand costing not just lives but also significant impact on the global economy. More than 212 countries and territories across the globe have been affected till now, causing approximately 4,043,068 confirmed cases and 277,016 deaths, and the numbers are rising expeditiously. The common symptoms are fever, dry cough, fatigue, sputum production, shortness of breath, sore throat, and headache, but atypical symptoms affecting cardiovascular system, gastrointestinal system, and neurological manifestations are not so uncommon and have been reported. Various governing bodies such as the World Health Organization, the National Institute for Health and Care Excellence, and the Centers for Disease Control and Prevention have laid down guidelines and recommendations for the same. In this review article, we have propounded the established knowledge about the causative agent in terms of epidemiological features, the etiopathogenesis, signs and symptoms, methods used for diagnosis, and the latest insight into the treatment and prevention according to the recent published recommendations. The current guidelines and the state-of-the-art updates for various active health-care personnel; allied departments such as radiology and microbiology, and those for the community have also been discussed.
Attitudes and practices of complementary and alternative medicine among patients attending primary care center in Saudi Arabia: A prospective cross-sectional study

Introduction and objectives: Estimated complementary and alternative medicine (CAM) usage in Saudi Arabia is 70%. The Saudi Food and Drug Authority, National Center for Complementary and Alternative Medicine (NCCAM) regulates the license-based practices of various CAM modalities including procedures, herbs, and devices. As an aid to help primary care physicians for understanding CAM practices among general population, this study aimed to explore the prevalence of CAM along with its most common modality, and to understand attitude and practices among patients attending primary care center in Riyadh (Saudi Arabia). Methods: A prospective cross-sectional study was conducted among 403 literate patients aged more than 18 years, coming to the primary care center for regular check-up from 1st April 2019 to 1st April 2020 Saudi Arabia. Data was collected using self-administered questionnaire. Results: Around 44.5% participants had practiced one method of alternative medicine in their life. Majority of participants who attended primary care centers belonged to the age group of 18–39 years (71.1%). The most common used method of alternative medicine was honey healing. More than half of the study participants had heard about the CAM from their family and friends. Nearly 45.7% had stated that the CAM cannot be used without consulting a medical professional. Nearly 3/4th population used it for treating up to 3 ailments. Conclusion: Use of alternative medicine was found to be prevalent in Riyadh. Family and friends were recognized as gatekeepers for propagating information about CAM usage in the society. Primary healthcare physicians need to be trained on prevalent practices of CAM to understand the societal needs.
Seasonality of pCO2 in a hard‐water lake of the northern Great Plains: The legacy effects of climate and limnological conditions over 36 years

Biogeochemical processes are active year‐round in ice‐covered lakes, such that processes in one season can affect limnological conditions in subsequent seasons. However, the extent and nature of these legacy effects are poorly understood, particularly for the CO2 content of lakes and when considering gas exchange with the atmosphere. Here, we used a unique 36‐yr dataset of weekly limnological measurements of Buffalo Pound Lake in the northern Great Plains to assess seasonal changes in CO2 concentration and flux and determine how dependent lake pCO2 is on limnological conditions of previous seasons. We found that the lake was a net source of CO2 to the atmosphere (mean 18.5 ± 7.4 mol CO2 m−2 yr−1), with spring potentially accounting for the majority (~ 64% ± 20%) of CO2 efflux, assuming ice in spring was permeable to gas exchange (32.9% ± 19.8% if not). Analysis with generalized additive models (GAMs) demonstrated that current and antecedent seasonal conditions combined to explain 72.6% of deviance in spring pCO2, but that the strength of model predictions and the importance of antecedent conditions diminished in GAMs of summer (43.6%) and fall (23.3%) CO2 levels. This research suggests that pCO2 is regulated by a combination of coeval and historical environmental conditions and shows that quantification of seasonal and annual fluxes requires a mechanistic understanding of the legacy effects of preceding time intervals.
Pulmonary Embolism Rate in Patients Infected with Severe Acute Respiratory Syndrome Coronavirus 2.

Our study demonstrated no difference in the rate of pulmonary embolism in patients with COVID-19 compared to patients presenting with similar symptoms without COVID-negative. Mounting evidence suggests that patients with severe COVID-19 have diffuse microthrombotic disease resulting in pulmonary parenchymal infarcts. Further studies testing this hypothesis will provide evidence to strengthen the guidelines for treating microthrombotic disease in patients with COVID-19 infection.
Salinosporamide A, a Marine-Derived Proteasome Inhibitor, Inhibits T Cell Activation through Regulating Proliferation and the Cell Cycle

The appropriate regulation of T cell activity under inflammatory conditions is crucial for maintaining immune homeostasis. Salinosporamide A discovered as a self-resistance product from the marine bacterium Salinospora tropica, has been used as a potent proteasome inhibitor (PI). Although PIs have been developed as novel therapeutics for autoimmune diseases, due to their immunosuppressive effect, whether salinosporamide A inhibits T cell activation remains unknown. The current study finds that salinosporamide A is not cytotoxic, but controls T cell proliferation. Results from our cell cycle arrest analysis revealed that salinosporamide A leads to cell cycle arrest and regulates the expression of cyclin-dependent kinases. Under activated conditions, salinosporamide A abrogated T cell activation by T cell receptor-mediated stimulation, in which the production of cytokines was inhibited by pretreatment with salinosporamide A. Furthermore, we demonstrated that the regulation of T cell activation by salinosporamide A is mediated by suppressing the MAPK pathway. Therefore, our results suggest that salinosporamide A effectively suppresses T cell activation through regulating T cell proliferation and the cell cycle and provides great insight into the development of novel therapeutics for autoimmune diseases or graft-versus-host disease.
Developing cell therapies as drug products

In the last 20 years, the global regulatory frameworks for drug assessment have been managing the challenges posed by using cellular products as new therapeutic tools. Currently, they are defined as “Advanced Therapy Medicinal Products”, comprising a large group of cellular types that either alone or in combination with gene and tissue engineering technology. They have the potential to change the natural course of still lethal or highly debilitating diseases, including cancers, opportunistic infections and chronic inflammatory conditions. Globally, more than 50 cell‐based products have obtained market authorization. This overview describes the advantages and unsolved challenges on developing cells as innovative therapeutic vehicles. The main cell therapy players and the legal framework are discussed, starting from chimeric antigen receptor T‐cells for leukaemia and solid tumours, dealing then with lymphocytes as potent anti‐microbiological tools and then focusing on mesenchymal stem/stromal cells whose role covers regenerative medicine, immunology and anti‐tumour therapy.
In-House Developed ELISA Indicates High Prevalence of Anti-Echinococcus granulosus IgG in Sheep Population—An Update from Pakistan

Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using probability proportional to size (PPS) statistical technique. Epidemiological information was recorded on a questionnaire for the estimation of potential risk factors. The serum samples were analyzed for IgG antibodies against Echinococcus granulosus using an in-house-developed EgAgB-based ELISA kit. The overall seroprevalence recorded was 21.98% (160/728) in the tested sheep, suggesting higher seropositivity in sheep from Punjab (23.73%) as compared to Khyber Pakhtunkhwa (KPK) (19.04%). The overall apparent prevalence observed by this ELISA method was almost similar to the calculated true prevalence (21.77%). Prevalence was significantly different (p < 0.05) among sheep from different districts. Higher prevalence was found in females (22.54%, OR 1.41), age group > 5 years (29.66%, OR 1.64), crossbreeds (42.85%, OR 2.70), and sheep with pasture access (25.96%, OR 3.06). Being in age group > 5 years and having pasture access were the factors significantly associated with seropositivity (p < 0.05). This study provides serological evidence of E. granulosus infection in sheep and can be used as a model for ante-mortem screening of the sheep globally.
Challenges Developing Deep Learning Algorithms in Cytology

Background: The incorporation of digital pathology into routine pathology practice is becoming more widespread. Definite advantages exist with respect to the implementation of artificial intelligence (AI) and deep learning in pathology, including cytopathology. However, there are also unique challenges in this regard. Summary: This review discusses cytology-specific challenges, including the need to implement digital cytology prior to AI; the large file sizes and increased acquisition times for whole slide images in cytology; the routine use of multiple stains, such as Papanicolaou and Romanowsky stains; the lack of high-quality annotated datasets on which to train algorithms; and the considerable computer resources required, in terms of both computer infrastructure and skilled personnel, for computing and storage of data. Global concerns regarding AI that are certainly applicable to cytology include the need for model validation and continued quality assurance, ethical issues such as the use of patient data in developing algorithms, the need to develop regulatory frameworks regarding what type of data can be utilized and ensuring cybersecurity during data collection and storage, and algorithm development. Key Messages: While AI will likely play a role in cytology practice in the future, applying this technology to cytology poses a unique set of challenges. A broad understanding of digital pathology and algorithm development is desirable to guide the development of algorithms, as well as the need to be cognizant of potential pitfalls to avoid when incorporating the technology in practice.
Dietary Patterns and the Risk of Prediabetes in Taiwan: A Cross-Sectional Study

Background: prediabetes prevention and management are the main methods used to combat the prevalence of diabetes. Exploratory factor analysis is an upcoming method that is successful in identifying dietary patterns that correlate with healthy or unhealthy outcomes. Aim: this study aims to identify dietary patterns in Taiwan that are associated with the risk of prediabetes. Methods: anthropometric, blood glucose, 3 d/24 h dietary records, and food frequency questionnaire data were collected from subjects recruited at Taipei Tzu-Chi Hospital. The following five dietary patterns were identified using factor analysis: Western, prudent, convenience, Asian traditional, and continental. This cross-sectional study compares tertiles of dietary patterns and analyzes the significance of the characteristics. Results: the Western and the prudent patterns are the major dietary patterns found in other studies. A higher factor loading in the Western pattern is significantly related to a higher risk of prediabetes. A higher factor loading in the continental pattern is significantly related to a lower risk of prediabetes. Conclusion: decreasing meat and seafood consumption while increasing egg, coffee, and milk consumption may be associated with a decreased risk for prediabetes.
Parallel empathy and group attitudes in late childhood: The role of perceived peer group attitudes

ABSTRACT Although several studies have examined outgroup empathy, the link between trait empathy and outgroup attitudes has been underinvestigated. In the present study this link was investigated among two samples of ethnic Dutch preadolescents (N = 335, Mage = 10.83 years, SD = 0.94; 53% girls; N = 326; Mage = 10.53 years, SD = 1.03; 48% girls). It examined children’s parallel empathy in relation to their ethnic attitudes, and the moderating role of perceived peer norms. Results (partly) support the hypotheses that empathy is associated with more outgroup positivity and less ingroup bias (ingroup minus outgroup attitude). The negative link between empathy and outgroup bias was stronger when peers were perceived to be more biased against the outgroup.
The Platelet Fraction Is a Novel Reservoir to Detect Lyme Borrelia in Blood

Simple Summary To diagnose Lyme disease, a patient’s blood is tested for antibodies that develop as part of the immune response. This can lead to cases being missed or inadequately treated. An ideal test would directly detect the Lyme disease bacteria, Borrelia, to provide better clinical guidance. In this study, we aimed to improve the methods currently used to find Borrelia in human blood, and identified two opportunities for optimization. We demonstrate that the container most commonly used to collect blood (EDTA) decreases Borrelia’s ability to grow, and we identify a superior alternative (citrate). Additionally, using experimentally infected blood, we show that Borrelia is highly concentrated in the platelet fraction, making it an ideal candidate for direct detection. These results lay the foundation for diagnostic test development, which could improve patient outcomes in Lyme disease. Abstract Serological diagnosis of Lyme disease suffers from considerable limitations. Yet, the technique cannot currently be replaced by direct detection methods, such as bacterial culture or molecular analysis, due to their inadequate sensitivity. The low bacterial burden in vasculature and lack of consensus around blood-based isolation of the causative pathogen, Borrelia burgdorferi, are central to this challenge. We therefore addressed methodological optimization of Borrelia recovery from blood, first by analyzing existing protocols, and then by using experimentally infected human blood to identify the processing conditions and fractions that increase Borrelia yield. In this proof-of-concept study, we now report two opportunities to improve recovery and detection of Borrelia from clinical samples. To enhance pathogen viability and cultivability during whole blood collection, citrate anticoagulant is superior to more commonly used EDTA. Despite the widespread reliance on serum and plasma as analytes, we found that the platelet fraction of blood concentrates Borrelia, providing an enriched resource for direct pathogen detection by microscopy, laboratory culture, Western blot, and PCR. The potential for platelets to serve as a reservoir for Borrelia and its diagnostic targets may transform direct clinical detection of this pathogen.
Mitochondrial UQCRC1 mutations cause autosomal dominant parkinsonism with polyneuropathy

See Cerri and Valente (doi:10.1093/brain/awaa324) for a scientific commentary on this article. Ubiquinol-cytochrome c reductase core protein I (UQCRC1) is a complex III core protein of the mitochondrial electron transport chain. Lin et al. identify rare missense mutations in mitochondrial UQCRC1 that segregate with Parkinson’s disease, and show that they are functionally pathogenic.