Miracle-dz/UniFAR-FTUs huggingface dataset:
Comprehensive Molecular Landscape of Cetuximab Resistance in Head and Neck Cancer Cell Lines

Cetuximab is the sole anti-EGFR monoclonal antibody that is FDA approved to treat head and neck squamous cell carcinoma (HNSCC). However, no predictive biomarkers of cetuximab response are known for HNSCC. Herein, we address the molecular mechanisms underlying cetuximab resistance in an in vitro model. We established a cetuximab resistant model (FaDu), using increased cetuximab concentrations for more than eight months. The resistance and parental cells were evaluated for cell viability and functional assays. Protein expression was analyzed by Western blot and human cell surface panel by lyoplate. The mutational profile and copy number alterations (CNA) were analyzed using whole-exome sequencing (WES) and the NanoString platform. FaDu resistant clones exhibited at least two-fold higher IC50 compared to the parental cell line. WES showed relevant mutations in several cancer-related genes, and the comparative mRNA expression analysis showed 36 differentially expressed genes associated with EGFR tyrosine kinase inhibitors resistance, RAS, MAPK, and mTOR signaling. Importantly, we observed that overexpression of KRAS, RhoA, and CD44 was associated with cetuximab resistance. Protein analysis revealed EGFR phosphorylation inhibition and mTOR increase in resistant cells. Moreover, the resistant cell line demonstrated an aggressive phenotype with a significant increase in adhesion, the number of colonies, and migration rates. Overall, we identified several molecular alterations in the cetuximab resistant cell line that may constitute novel biomarkers of cetuximab response such as mTOR and RhoA overexpression. These findings indicate new strategies to overcome anti-EGFR resistance in HNSCC.
Landmark information included in turn-by-turn instructions induce incidental acquisition of lasting route knowledge

ABSTRACT The augmentation of landmarks in auditory navigation instructions has been shown to improve incidental spatial knowledge acquisition during assisted navigation. Here, two driving simulator experiments are reported that replicated this effect even when adding a three-week delay between navigation and spatial tasks and varying the degree of detail in the provided landmark information. Performance in free- and cued-recall of landmarks and driving the route again without assistance demonstrated increased landmark and route knowledge when navigating with landmark-based compared to common GPS instructions. The results emphasize that adding auditory landmark references to existing navigation systems can potentiate processing of spatial features of the environment during every-day navigation.
Androgen Receptor as an Emerging Feasible Biomarker for Breast Cancer

Biomarkers can be used for diagnosis, prognosis, and prediction in targeted therapy. The estrogen receptor α (ERα) and human epidermal growth factor receptor 2 (HER2) are standard biomarkers used in breast cancer for guiding disease treatment. The androgen receptor (AR), a nuclear hormone receptor, contributes to the development and progression of prostate tumors and other cancers. With increasing evidence to support that AR plays an essential role in breast cancer, AR has been considered a useful biomarker in breast cancer, depending on the context of breast cancer sub-types. The existing survival analyses suggest that AR acts as a tumor suppressor in ER + ve breast cancers, serving as a favorable prognostic marker. However, AR functions as a tumor promoter in ER-ve breast cancers, including HER2 + ve and triple-negative (TNBC) breast cancers, serving as a poor prognostic factor. AR has also been shown to be predictive of the potential of response to adjuvant hormonal therapy in ER + ve breast cancers and to neoadjuvant chemotherapy in TNBC. However, conflicting results do exist due to intrinsic molecular differences between tumors and the scoring method for AR positivity. Applying AR expression status to guide treatment in different breast cancer sub-types has been suggested. In the future, AR will be a feasible biomarker for breast cancer. Clinical trials using AR antagonists in breast cancer are active. Targeting AR alone or other therapeutic agents provides alternatives to existing therapy for breast cancer. Therefore, AR expression will be necessary if AR-targeted treatment is to be used.
Understanding Political Efficacy among Asian American Undergraduates at Research Universities

In this article, we set out to identify college experiences, institutional climate factors, and student characteristics that relate to political efficacy among Asian American undergraduates. We analyze data from 3,705 Asian American undergraduates at six research intensive universities from the 2017 Student Experience in the Research University survey. We discuss directions for future research and implications for administrators, faculty, and student affairs practitioners to better promote political efficacy development among Asian American undergraduates.
Role of Geography and Pedagogical Approaches used in the Training of Pre-service Teachers in Kenyan Universities: A Case of Kenyatta University, Kenya

In which countries are elephants found! The discipline of Geography is as big as an elephant and as thick as the equatorial forest. The question of how Geography should be taught has been an agonizing pedagogical issue among educators of the discipline. Geography is an important discipline in the school curriculum and its connectedness with other disciplines taught in schools outweighs its role in educating the young in schools today. If taught to meet the said objectives of teaching Geography, then the discipline enables learners to meet the national goals of education, equips the learners with useful life skills and professional attitude that meets the global labor market demands. This paper seeks to establish the role of Geography, the existing pedagogical strategies and methods of training that teacher training institutions in Kenya use to prepare novice teachers who teach in secondary schools. It further examines the framework of conceptualizing school Geography within the school curriculum in order to prepare learners for challenges that befall them long after schooling. School Geography therefore as it applies in a learning context means: learnt concepts, knowledge and skills within the national goals of educational and levels of assignment designed to prepare the learner for the future world of work as well as the learner having knowledge to relate with the environment. The study used an exploratory survey approach to collect data from 435 pre-service teacher trainees at Kenyatta University. The current pedagogical strategies used have not achieved the high global levels of training that adequately equip the teachers for their professional works that is meant to equip the learners with content and high level skills that meet the global labor market demands and the expected threshold in society. It is important to note that this paper further establishes numerous challenges experienced in the course of training and acquisition of knowledge and skills. The study has proposed the use of American Humane Association model for adaption make its contributions in the creation of knowledge and skills in the field of education and beyond. exploratory survey study sought to establish the pedagogical strategies, that is, the modes of delivery and knowledge acquisition processes envisaged in the Kenyan University training institutions that prepare teachers for schools. The study used 435 pre-service teachers undergoing training in Bachelor of education to teach Geography in schools. The researcher used questionnaires and interviews to collect data. The study addressed the styles adapted for training, learning and the challenges experienced by both the lecturers and the students in their quest for knowledge and skills to meet the expected career goals.
JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency

Obstructive arterial diseases, including supravalvular aortic stenosis (SVAS), atherosclerosis, and restenosis, share 2 important features: an abnormal or disrupted elastic lamellae structure and excessive smooth muscle cells (SMCs). However, the relationship between these pathological features is poorly delineated. SVAS is caused by heterozygous loss-of-function, hypomorphic, or deletion mutations in the elastin gene (ELN), and SVAS patients and elastin-mutant mice display increased arterial wall cellularity and luminal obstructions. Pharmacological treatments for SVAS are lacking, as the underlying pathobiology is inadequately defined. Herein, using human aortic vascular cells, mouse models, and aortic samples and SMCs derived from induced pluripotent stem cells of ELN-deficient patients, we demonstrated that elastin insufficiency induced epigenetic changes, upregulating the NOTCH pathway in SMCs. Specifically, reduced elastin increased levels of γ-secretase, activated NOTCH3 intracellular domain, and downstream genes. Notch3 deletion or pharmacological inhibition of γ-secretase attenuated aortic hypermuscularization and stenosis in Eln–/– mutants. Eln–/– mice expressed higher levels of NOTCH ligand JAGGED1 (JAG1) in aortic SMCs and endothelial cells (ECs). Finally, Jag1 deletion in SMCs, but not ECs, mitigated the hypermuscular and stenotic phenotype in the aorta of Eln–/– mice. Our findings reveal that NOTCH3 pathway upregulation induced pathological aortic SMC accumulation during elastin insufficiency and provide potential therapeutic targets for SVAS.
Seasonal dynamics of Diptera in individual biotopes in the center of the European part of Russia

In a changing climate, phenological observations are gaining new importance. They can tell what changes are taking place in certain environmental conditions. The studies were conducted in 2019 within the territory of the Republic of Mordovia (the center of the European part of Russia). Beer traps (beer as a bait) were used to collect Diptera. The material was collected in the period from April to October in different forest biotopes (pine forest, lime forest, aspen forest, birch forest and oak forest) and the air temperature was recorded at the same time. In total, more than 14.000 specimens of Diptera were recorded. Overall, 29 families were recorded. The largest number of families was observed for birch (23 families) and pine (24 families) forests, the smallest number – in aspen forest (16 families). The families Muscidae, Drosophilidae, Calliphoridae had the largest number of captured individuals (44.5%, 35.2%, 7.6% of the total number of individuals respectively). The highest number of individuals was captured in oak forest. The dynamics of abundance in all biotopes were similar and were characterized by the same number of declines and rises. The first small significant peak in the number of Diptera occurred in the first half of summer. A slight increase in the number of specimenі occurred in mid-June. In the second half of September, there was a gradual increase in the number and the maximum peak was recorded in mid-October, then there was a decline. The autumn increase in the number of Diptera in all five biotopes exceeded the summer peak by several times. This dynamic was typical for most families. However, species from the family Lonchaeidae had the peak in July. For our better understanding of the changes in the seasonal dynamics of the number of Diptera, long-term observations in different climatic zones are needed.
Consequences of Social and Environmental Corporate Responsibility Practices: Managers’ Perception in Mozambique

The objective of this paper is to analyze the relationship between the social and environmental practices of Corporate Social Responsibility (CSR), and the economic–financial, social, and environmental performance in Mozambican companies, from the managers’ perspectives. The data were collected from a sample of 227 companies through a survey questionnaire. We used structural equation modelling to analyze how the managers correlate the different social and environmental practices with performance at the financial, social, and environmental levels. The results showed that the relationship between all major components of the social and environmental practices, and the economic–financial, social, and environmental performance is positive but insignificant with the exception of the social practices of community support, which has a weak relationship with the economic–financial performance, environmental performance, and social performance, as well as the environmental practices. The data indicate that there is a need for strengthening the appropriate economic–financial incentive policies and strategies for the agents who promote good CSR practices in the country, in order to obtain satisfactory, measurable, and comparable economic–financial, social, and environmental performance.
Ecological impacts of shortening fire return intervals on boreal peatlands and transition zones using integrated in situ field sampling and lidar approaches

Aridity associated with rising air temperatures in northern latitudes is expected to contribute to increased frequency of wildland fires. Here, we examined regenerating vegetation following short return interval (SRI) fire (56 years post‐fire) compared to long return interval (LRI) fire (>80 years post‐fire) in boreal peatlands and their adjacent transitional areas. The objectives of this study were to quantify if differences exist between (1) peatland and transitional soil characteristics in LRI versus SRI areas and (2) regenerating vegetation species, structural characteristics and diversity. We also determined if patterns of vegetation structural characteristics observed using field data also occur across the broader landscape using airborne lidar data. The Utikuma Region Study Area (URSA) is located in central Alberta, Canada. Here, 19 peatlands were sampled, coincident with an airborne lidar survey of the broader region, where 120 peatlands in short and long fire return intervals were identified. We found that SRI transitional areas had significantly deeper organic soil deposits than those found in LRI (p < 0.0001). Proportions of regenerating species differed significantly between peatlands and transitional areas in SRI versus LRI, where greater proportion of coniferous species were observed in LRI. Deciduous transitional–upland species and taller post‐fire vegetation heights were more commonly found SRI peatlands compared with LRI. This suggest that fires with SRIs in this region may result in enhanced deciduous succession, which may transition boreal peatlands into ecosystems that have some characteristics of transitional and upland forests.
Prediction of Recovery from Traumatic Brain Injury with EEG Power Spectrum in Combination of Independent Component Analysis and RUSBoost Model

The computational electroencephalogram (EEG) is recently garnering significant attention in examining whether the quantitative EEG (qEEG) features can be used as new predictors for the prediction of recovery in moderate traumatic brain injury (TBI). However, the brain’s recorded electrical activity has always been contaminated with artifacts, which in turn further impede the subsequent processing steps. As a result, it is crucial to devise a strategy for meticulously flagging and extracting clean EEG data to retrieve high-quality discriminative features for successful model development. This work proposed the use of multiple artifact rejection algorithms (MARA), which is an independent component analysis (ICA)-based algorithm, to eliminate artifacts automatically, and explored their effects on the predictive performance of the random undersampling boosting (RUSBoost) model. Continuous EEG were acquired using 64 electrodes from 27 moderate TBI patients at four weeks to one-year post-accident. The MARA incorporates an artifact removal stage based on ICA prior to RUSBoost, SVM, DT, and k-NN classification. The area under the curve (AUC) of RUSBoost was higher in absolute power spectral density (PSD) in AUCδ = 0.75, AUC α = 0.73 and AUCθ = 0.71 bands than SVM, DT, and k-NN. The MARA has provided a good generalization performance of the RUSBoost prediction model.
Discretionary loan loss provision in the Moroccan banking sector: The role of governance mechanisms

Abstract Purpose: The present paper aims to examine the impact of corporate governance mechanisms on earnings management extent in the Moroccan banking sector. Research methodology: This research investigates the relationship between listed banks' governance mechanisms and earnings management in the CSE over the period 2017-2020. This study relies on a two-step quantitative approach, which consists firstly of estimating discretionary loan loss provisions to measure EM, then presenting the association between banks’ governance mechanisms and discretionary loan loss provisions. Results: The findings indicate that board size, gender diversity, audit committee’s independence, and state ownership constraint EM practices among the Moroccan listed banks. While other governance mechanisms, such as institutional ownership and board activity, seem to have no significant effect on restraining managers’ discretionary behavior. Limitations: Many qualitative and quantitative factors could influence discretionary loan loss provisions and not only the used variables in this research. Contribution: This research reveals the need to maintain the vigilant supervision of the regulatory framework to limit these opportunistic practices in the local banking industry. Also, our study has important implications for establishing a new set of governance requirements such as board diversity in Morocco.
Genomic dissection of the microevolution of Australian epidemic Bordetella pertussis

Whooping cough (pertussis) is a highly contagious respiratory disease caused by the bacterium Bordetella pertussis. Despite high vaccine coverage, pertussis has re-emerged in many countries and caused two large epidemics in Australia since 2007. Here, we undertook a genomic and phylogeographic study of 385 Australian B. pertussis isolates collected from 2008 to 2017. The Australian B. pertussis population was found to be composed of mostly ptxP3 strains carrying different fim3 alleles, with ptxP3-fim3A genotype expanded far more than ptxP3-fim3B. Within the former, there were six co-circulating epidemic lineages (EL1 to EL6). The multiple ELs emerged, expanded, and then declined at different time points over the two epidemics, likely driven by immune selection from pertussis vaccination and natural infection in addition to local and global transmission events. Both hard and soft selective sweeps through vaccine selection pressures determined the current B. pertussis population dynamics. Relative risk analysis found that once a new B. pertussis lineage emerged, it was more likely to spread locally within the first 1.5 years. However, after 1.5 years, any new lineage was likely to expand to a wider region and became no longer spatially structured across the country. Phylogenetic analysis revealed the expansion of ptxP3 strains was also associated with replacement of the type III secretion system allele bscI1 with bscI3. This study advanced our understanding of the epidemic population structure and spatial and temporal dynamics of B. pertussis in a highly immunised population.
From Experience to Text: Issues of Representation, Disclosure, and Understanding in Ethnographic Social Work Research

ABSTRACT This paper starts with the ethical dilemma that appears when researchers end data collection, start to analyse the material, and construct the narrative of the thesis. This is the moment when the research subject might become objectified. Although this is a well-recognised dilemma, the aim of this paper is to investigate this in relation to ethnographic studies in social work in order to further develop some aspects of this ‘old’ dilemma. Three prominent texts on the topics of representation, disclosure, and understanding are used in an analysis of two ethnographic observations in different social work settings. A conclusion is that the main challenge for the ethnographic researcher in social work is the fact that they often write concurrently about people in unequal positions. Hierarchies and power relations have to be part of the question about how to write without objectifying. This fact places the researcher in a position of ambiguity because they act on both sides, including both those who are in privileged positions and those who are considered vulnerable groups. Hence, the ethical dilemma not only includes the relation between the researcher and the research subject, but also inter-party relations between research subjects in different power positions.
Soluble cuticular wax composition and antimicrobial activity of the fruits of Chaenomeles species and an interspecific hybrid

Plants of the genus Chaenomeles Lindl. (Rosaceae) naturally grow in Southeast Asia and represent the richest resource of biologically active compounds with beneficial properties for humans. Plants of C. japonica (Thunb.) Lindl. and C. speciosa (Sweet) Nakai species, and interspecific hybrid C. × superba (Frahm) Rehder (C. japonica × C. speciosa, Superba group) have been successfully introduced in the steppe zone of Ukraine and bear fruits. In this study, we evaluated chemical composition of fruit cuticular waxes and antimicrobial activity of fruit extracts. The soluble waxes were characterized using gas chromatography-mass spectrometry (GC-MS), and 26–36 compounds, representing 91.7–96.6% of the total soluble cuticular waxes, were identified. Waxes of Chaenomeles fruits belonged to six classes, namely fatty acids, alcohols, aldehydes, esters, ethers and alkanes. Aldehydes 7-hexadecenal and heptacosanal, and alkanes hexatriacontane and tetrapentacontane were the main constituents in the soluble cuticular waxes of C. speciosa and C. × superba fruits, accounting for more than half of the total contents. However, alkane tetrapentacontane, alcohol 8,10-hexadecadien-1-ol and heptacosanal prevailed in C. japonica fruit waxes. Isopropanolic fruit extracts exhibited dose-dependent antimicrobial activity against four Gram-negative bacteria, five Gram-positive bacteria and one fungal strain in the disc diffusion assay. In general, extracts from the Chaenomeles fruits demonstrated higher activity against Gram+ bacteria than Gram- strains. The strongest inhibiting activity was shown against Staphylococcus epidermidis (by the fruit extracts of C. × superba and C. speciosa), Micrococcus lysodeikticus and Candida albicans (both by C. × superba fruit extract). Results of the study confirmed accumulation of the bioactive compounds in the fruit waxes of different Chaenomeles species and antimicrobial ability of Chaenomeles fruits as well. These findings revealed the bioactive compounds in fruit cuticular waxes and suggested health-promoting properties of introduced Chaenomeles species.