Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytomorphometric Evaluation of Oral Exfoliated Cells − Its Correlation With Age of an Individual

Introduction: Age is a vital tool in assessing the mental and physical growth of an individual. Exfoliative cytology, a non-invasive procedure, also has its potential implication in age assessment of an individual. This study aims to correlate and compare the average cell size from oral buccal smears of individuals of varying age groups using cytomorphometric analysis. Materials and Methods: In this study, 100 buccal smears were collected from normal individuals, dividing them into five age groups (Group 1: 10–20, Group 2: 21–30, Group 3: 31–40, Group 4: 41–50, Group 5: 50 and above). The collected smear samples were stained using papanicolau stain. The average sizes of the cells were measured using Dewinter’s image analysis software. Results: Cytomorphometry revealed a decrease in the average cell size as age increases. Analysis using Bonferonni and Post Hoc tests showed statistically significant decrease in the average cell size (P < 0.005). Conclusion: Cytomorphometric evaluation of exfoliated oral cells proves reliable tool for correlation of age. Cytomorphometric analysis of exfoliated cells of buccal mucosa serves a potential alternative non-invasive procedure in evaluation and correlation of age of an individual compared to the other screening modalities, which are usually either invasive or expensive.
The Psychophysiological Effects of Different Tempo Music on Endurance Versus High-Intensity Performances

The use of music during training represents a special paradigm for trainers to stimulate people undertaking different types of exercise. However, the relationship between the tempo of music and perception of effort during different metabolic demands is still unclear. Therefore, the aim of this research was to determine whether high intensity exercise is more sensitive to the beneficial effects of music than endurance exercise. This study assessed 19 active women (age 26.4 ± 2.6 years) during endurance (walking for 10′ at 6.5 km/h on a treadmill) and high intensity (80% on 1-RM) exercise under four different randomly assigned conditions: no music (NM), with music at 90–110 bpm (LOW), with music at 130–150 bpm (MED), and with music at 170–190 bpm (HIGH). During each trial, heart rate (HR) and the rating of perceived exertion (RPE) were assessed. Repeated analysis of variance measures was used to detect any differences between the four conditions during high intensity and low intensity exercise. RPE showed more substantial changes during the endurance exercises (11%), than during high intensity exercise (6.5%), between HIGH and NM conditions. The metabolic demand during the walking exercise increased between NM and HIGH bpm conditions. This study indicates the benefits of music under stress conditions as well as during endurance and high intensity training. The results demonstrate that the beneficial effects of music are more likely to be seen in endurance exercise. Consequently, music may be considered an important tool to stimulate people engaging in low intensity physical exercise.
Functional Outcomes and Reading Performance After Combined Implantation of a Small-Aperture Lens and a Segmental Refractive Bifocal Lens.

PURPOSE Clinical evaluation of the visual outcomes after implantation of a small-aperture extended depth of focus (EDOF) intraocular lens (IOL) and a segmental refractive bifocal lens. METHODS In this prospective study, 13 patients with cataract received a small-aperture IC-8 IOL (AcuFocus, Irvine, CA) in one eye and a segmental-refractive Lentis Mplus LS-313 MF20 IOL (Oculentis, Berlin, Germany) in the fellow eye. The clinical examination included measurements of uncorrected (UDVA) and corrected (CDVA) distance visual acuity, uncorrected (UIVA) and distance-corrected (DCIVA) intermediate visual acuity, and uncorrected (UNVA) and distance-corrected (DCNVA) near visual acuity. Additionally, a defocus curve was obtained from +2.00 to -5.00 diopters (D) and a Salzburg Reading Desk was used to assess the patients' reading acuity at intermediate and near distances. A halo and glare simulator was used to evaluate the postoperative perception of dysphotopsia. RESULTS At 5 months postoperatively, the mean binocular visual results demonstrated UDVA, UIVA, and UNVA values of -0.04 ± 0.11, 0.00 ± 0.10, and 0.11 ± 0.08 logMAR, respectively. The binocular distance-corrected reading performance test also confirmed the improved visual function, with an intermediate reading acuity of 0.12 logMAR at 69.21 cm and a near reading acuity of 0.19 logMAR at 41.63 cm. The mean halo size was 32.54 ± 22.38, mean halo intensity was 34.46 ± 21.95, mean glare size was 9.00 ± 17.47, and mean glare intensity was 9.92 ± 16.84. CONCLUSIONS The new concept of a combined implantation of a small-aperture IOL and a segmental-refractive bifocal lens showed good results in far and intermediate distances and functional results at near distance, while causing minimal photic phenomena. [J Refract Surg. 2019;35(9):551-558. ].
Long-Term Patient-Reported Quality of Life After Stereotactic Body Radiation Therapy for Recurrent, Previously-Irradiated Head and Neck Cancer

Objectives: Long-term quality-of-life data following stereotactic body radiation therapy (SBRT) for recurrent head and neck cancer (rHNC) is underreported. We report patient-reported quality-of-life (PR-QOL) after at least 1 year post-treatment. Methods and Materials: A retrospective review was performed on 64 patients receiving SBRT for previously-irradiated rHNC. PR-QOL was prospectively evaluated using the University of Washington Quality of Life Questionnaire. The mixed effects proportional odds model was used to assess post-treatment overall PR-QOL changes, as well as the effects of late toxicities, tumor volume > 25 cc, local failure, nodal recurrence, distant failure, prior neck dissection, performance status other than ECOG 0 or Karnofsky 100, sex, age >65, squamous vs. non-squamous primary histology, and specific organ recurrence. Results: SBRT had no significant effect on overall PR-QOL at days 1-90 post-treatment (SBRT effect 0.035, p = 0.93) and days 91–365 (SBRT effect −0.30, p = 0.45). Beyond day 365, overall PR-QOL was significantly worse than baseline (SBRT effect −0.77, p =.03). Grade ≥3 late toxicities (p = 0.0072) and tumor volume > 25 cc (p = 0.032) predicted significantly worse overall PR-QOL. Oral cavity recurrence predicted significant decrements in chewing (p = 0.0006), swallowing (p = 0.0301), and taste PR-QOL (p = 0.02). Nasal recurrence predicted significant decrements in taste PR-QOL (p = 0.030). Grade ≥3 late dysphagia predicted significant decline in chewing (p = 0.039) and swallowing (p = 0.0004). Grade ≥3 late osteonecrosis predicted significant differences in pain PR-QOL (p = 0.0026). Conclusion: PR-QOL across several domains declines immediately after SBRT for previously-irradiated rHNC before returning to baseline levels at 1 year. Long-term PR-QOL declines thereafter. Patients with grade ≥3 late toxicities or tumor volume >25 cc report reduced long-term overall PR-QOL, likely representing late disease progression. Specific organ recurrence and grade ≥3 late toxicities predict decrements in specific PR-QOL domains.
The Functional Neuroanatomy of Developmental Dyslexia Across Languages and Writing Systems

The present article reviews the literature on the functional neuroanatomy of developmental dyslexia across languages and writing systems. This includes comparisons of alphabetic languages differing in orthographic depth as well as comparisons across alphabetic, syllabic, and logographic writing systems. It provides a synthesis of the evidence for both universal and language-specific effects on dyslexic functional brain activation abnormalities during reading and reading-related tasks. Specifically, universal reading-related underactivation of dyslexic readers relative to typical readers is identified in core regions of the left hemisphere reading network including the occipito-temporal, temporo-parietal, and inferior frontal cortex. Orthography-specific dyslexic brain abnormalities are mainly related to the degree and spatial extent of under- and overactivation clusters. In addition, dyslexic structural gray matter abnormalities across languages and writing systems are analyzed. The neuroimaging findings are linked to the universal and orthography-dependent behavioral manifestations of developmental dyslexia. Finally, the present article provides insights into potential compensatory mechanisms that may support remediation across languages and writing systems.
Cytoarchitectonic Characterization and Functional Decoding of Four New Areas in the Human Lateral Orbitofrontal Cortex

A comprehensive concept of the biological basis of reward, social and emotional behavior, and language requires a deeper understanding of the microstructure and connectivity of the underlying brain regions. Such understanding could provide deeper insights into their role in functional networks, and form the anatomical basis of the functional segregation of this region as shown in recent in vivo imaging studies. Here, we investigated the cytoarchitecture of the lateral orbitofrontal cortex (lateral OFC) in serial histological sections of 10 human postmortem brains by image analysis and a statistically reproducible approach to detect borders between cortical areas. Profiles of the volume fraction of cell bodies were therefore extracted from digitized histological images, describing laminar changes from the layer I/layer II boundary to the white matter. As a result, four new areas, Fo4–7, were identified. Area Fo4 was mainly found in the anterior orbital gyrus (AOG), Fo5 anteriorly in the inferior frontal gyrus (IFG), Fo6 in the lateral orbital gyrus (LOG), and Fo7 in the lateral orbital sulcus. Areas differed in cortical thickness, abundance and size of pyramidal cells in layer III and degree of granularity in layer IV. A hierarchical cluster analysis was used to quantify cytoarchitectonic differences between them. The 3D-reconstructed areas were transformed into the single-subject template of the Montreal Neurological Institute (MNI), where probabilistic maps and a maximum probability map (MPM) were calculated as part of the JuBrain Cytoarchitectonic Atlas. These maps served as reference data to study the functional properties of the areas using the BrainMap database. The type of behavioral tasks that activated them was analyzed to get first insights of co-activation patterns of the lateral OFC and its contribution to cognitive networks. Meta-analytic connectivity modeling (MACM) showed that functional decoding revealed activation in gustatory perception in Fo4; reward and somesthetic perception in Fo5; semantic processing and pain perception in Fo6; and emotional processing and covert reading in Fo7. Together with existing maps of the JuBrain Cytoarchitectonic Atlas, the new maps can now be used as an open-source reference for neuroimaging studies, allowing to further decode brain function.
The Overlap of Poor Reading Comprehension in English and French

This study examined overlap and correlates of poor reading comprehension in English and French for children in early French immersion. Poor comprehenders were identified in grade 3 in English and French using a regression method to predict reading comprehension scores from age, non-verbal reasoning, word reading accuracy, and word reading fluency. Three groups of poor comprehenders were identified: 10 poor comprehenders in English and French, 11 poor comprehenders in English, and 10 poor comprehenders in French, and compared to 10 controls with good reading comprehension in both English and French. There was a moderate degree of overlap in comprehension difficulties in English and French among poor comprehenders with equivalent amounts of exposure to French, with a prevalence rate of 41.7% in our sample. Children who were poor comprehenders in both English and French consistently scored the lowest on English vocabulary in grade 1 and grade 3 and in French vocabulary in grade 3 suggesting that poor comprehenders’ vocabulary weaknesses in English as a primary language may contribute to comprehension difficulties in English and French.
Cyp2j5-Gene Deletion Affects on Acetylcholine and Adenosine-Induced Relaxation in Mice: Role of Angiotensin-II and CYP-Epoxygenase Inhibitor

Previously, we showed vascular endothelial overexpression of human-CYP2J2 enhances coronary reactive hyperemia in Tie2-CYP2J2 Tr mice, and eNOS−/− mice had overexpression of CYP2J-epoxygenase with adenosine A2A receptor-induced enhance relaxation, but we did not see the response in CYP2J-epoxygenase knockout mice. Therefore, we hypothesized that Cyp2j5-gene deletion affects acetylcholine- and 5'-N-ethylcarboxamidoadenosine (NECA) (adenosine)-induced relaxation and their response is partially inhibited by angiotensin-II (Ang-II) in mice. Acetylcholine (Ach)-induced response was tested with N-(methylsulfonyl)-2-(2-propynyloxy)-benzenehexanamide (MS-PPOH, CYP-epoxygenase inhibitor; 10−5M) and Ang-II (10−6M). In Cyp2j5−/− mice, ACh-induced relaxation was different from C57Bl/6 mice, at 10−5 M (76.1 ± 3.3 vs. 58.3 ± 5.2, P < 0.05). However, ACh-induced relaxation was not blocked by MS-PPOH in Cyp2j5−/−: 58.5 ± 5.0%, P > 0.05, but blocked in C57Bl/6: 52.3 ± 7.5%, P < 0.05, and Ang-II reduces ACh-induced relaxation in both Cyp2j5−/− and C57Bl/6 mice (38.8 ± 3.9% and 45.9 ± 7.8, P <0.05). In addition, NECA-induced response was tested with Ang-II. In Cyp2j5−/− mice, NECA-induced response was not different from C57Bl/6 mice at 10−5M (23.1 ± 2.1 vs. 21.1 ± 3.8, P > 0.05). However, NECA-induced response was reduced by Ang-II in both Cyp2j5−/− and C57Bl/6 mice (−10.8 ± 2.3% and 3.2 ± 2.7, P < 0.05). Data suggest that ACh-induced relaxation in Cyp2j5−/− mice depends on nitric oxide (NO) but not CYP-epoxygenases, and the NECA-induced different response in male vs. female Cyp2j5−/− mice when Ang-II treated.
Striatum-Mediated Deficits in Stimulus-Response Learning and Decision-Making in OCD

Obsessive compulsive disorder (OCD) is a prevalent psychiatric disorder characterized by obsessions and compulsions. Studies investigating symptomatology and cognitive deficits in OCD frequently implicate the striatum. The aim of this study was to explore striatum-mediated cognitive deficits in patients with OCD as they complete a stimulus-response learning task previously shown to differentially rely on the dorsal (DS) and ventral striatum (VS). We hypothesized that patients with OCD will show both impaired decision-making and learning, coupled with reduced task-relevant activity in DS and VS, respectively, compared to healthy controls. We found that patients with OCD (n = 14) exhibited decision-making deficits and learned associations slower compared to healthy age-matched controls (n = 16). Along with these behavioral deficits, OCD patients had reduced task-relevant activity in DS and VS, compared to controls. This study reveals that responses in DS and VS are altered in OCD, and sheds light on the cognitive deficits and symptoms experienced by patients with OCD.
sraX: A Novel Comprehensive Resistome Analysis Tool

The accurate identification of the assortment of antibiotic resistance genes within a collection of genomes enables the discernment of intricate antimicrobial resistance (AMR) patterns while depicting the diversity of resistome profiles of the analyzed samples. The availability of large amount of sequence data, owing to the advancement of novel sequencing technologies, have conceded exciting possibilities for developing suitable AMR exploration tools. However, the level of complexity of bioinformatic analyses has raised as well, since the achievement of desired results involves executing several challenging steps. Here, sraX is proposed as a fully automated analytical pipeline for performing a precise resistome analysis. Our nominated tool is capable of scrutinizing hundreds of bacterial genomes in-parallel for detecting and annotating putative resistant determinants. Particularly, sraX presents unique features: genomic context analysis, validation of known mutations conferring resistance, illustration of drug classes and type of mutated loci proportions and integration of results into a single hyperlinked navigable HTML-formatted file. Furthermore, sraX also exhibits relevant operational features since the complete analysis is accomplished by executing a single-command step. The capacity and efficacy of sraX was demonstrated by re-analyzing 197 strains belonging to Enterococcus spp. , from which we confirmed 99.15% of all detection events that were reported in the original study. sraX can be downloaded from https://github.com/lgpdevtools/srax.
Classical Swine Fever in a Cuban Zone Intended for Eradication: Spatiotemporal Clustering and Risk Factors

Classical Swine Fever (CSF) is an endemic disease in Cuba, and an eradication strategy by zones is planned by the Official Veterinary Service. The aim of this study was to identify high-risk areas of CSF and the risk factors associated with the disease occurrence in the Pinar del Río province, one of the prioritized areas in the eradication strategy. The outbreak occurrence at district level was analyzed through a 7-year period (2009 to 2015). A high-risk cluster (RR = 5.13, 95% CI 3.49–7.56) was detected during the last 2 years of the study period in the eastern half of the province, with 38 out of 97 districts included. The rate of CSF-affected holdings had a significant increase during 2014–2015 and seems to have occurred mainly in the high-risk cluster area. Swine population density by district (heads/km2) and road length (km) by district were associated with the disease outbreak occurrence. These results provide new insights into the knowledge of the epidemiology of the disease in Cuban endemic conditions and can contribute to improving the control and the eradication strategy in this situation.
Seismometers Within Cities: A Tool to Connect Earth Sciences and Society

The high degree of human activities in urban environments produces large background vibrations that makes it difficult to use data acquired in these areas for classical seismology. Seismometers installed within cities have been typically been used for the study of seismic hazard or for monitoring civil engineering problems. However, with the development of monitoring techniques based on the interpretation of the so-called seismic ambient noise, these data have gained scientific interest. Our objective is to discuss an additional utility of seismometers deployed within a city; its use as a tool to connect society with Earth sciences. Many citizen activities, from traffic to music concerts, produce vibrations that can be recorded seismically, and our experience shows that these records attract the attention of the media and social networks. With the emergence of low-cost and easy-to-use instruments in recent years, more citizens can now record ground motion and become interested in the interpretation of the recorded seismograms. The installation of permanent seismic networks in educational centers has proven to be a good approach to introduce students to Earth sciences at the national level and can also be developed at the urban scale using this new instrumentation. In this contribution we will first review the previous results related to the identification of the sources of vibration in urban areas and then present a new ongoing project based on the deployment of a seismic network in educational centers located in the city of Barcelona.
Increased CSF Soluble TREM2 Concentration in Patients With Neurosyphilis

Objective: To explore cerebrospinal fluid (CSF) levels of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and neurofilament light proteins (NFL) in patients with neurosyphilis (NS). Methods: We enrolled 71 NS patients (41 early-NS and 30 late-NS patients) and 20 syphilis but non-NS patients whose CSF samples were collected. The CSF levels of the microglial activation biomarker sTREM2 and neuronal injury biomarker NFL were measured using ELISA. Results: CSF sTREM2 levels were significantly higher in NS patients compared to those in syphilis/non-NS patients (p < 0.001). In a subgroup analysis, the CSF sTREM2 levels elevated significantly in late-NS patients than those in early-NS patients (p < 0.001). The CSF sTREM2 levels in early-NS group were also significantly higher than those in syphilis/non-NS group (p = 0.024). Like CSF sTREM2, similar differences between groups were also found in CSF NFL. There was a moderate correlation between CSF sTREM2 and CSF NFL (r = 0.406, p < 0.001) in NS group. Conclusions: CSF sTREM2 levels elevated in NS and peaked at the late stage, suggesting that CSF sTREM2 may be a useful marker to quantify microglia activation in NS and may play a role in the progression of NS. The positive correlation between CSF sTREM2 and CSF NFL indicates a linkage between microglial activation and neuronal injury in NS.
Simple Skeletal Muscle Mass Estimation Formulas: What We Can Learn From Them

One century ago Harris and Benedict published a short report critically examining the relations between body size, body shape, age, and basal metabolic rate. At the time, basal metabolic rate was a vital measurement in diagnosing diseases such as hypothyroidism. Their conclusions and basal metabolic rate prediction formulas still resonate today. Using the Harris-Benedict approach as a template, we systematically examined the relations between body size, body shape, age, and skeletal muscle mass (SM), the main anatomic feature of sarcopenia. The sample consisted of 12,330 non-Hispanic (NH) white and NH black participants in the US National Health and Nutrition Survey who had complete weight, height, waist circumference, age, and dual-energy X-ray (DXA) absorptiometry data. A conversion formula was used to derive SM from DXA-measured appendicular lean soft tissue mass. Weight, height, waist circumference, and age alone and in combination were significantly correlated with SM (all, p < 0.001). Advancing analyses through the aforementioned sequence of predictor variables allowed us to establish how at the anatomic level these body size, body shape, and age measures relate to SM much in the same way the Harris-Benedict equations provide insights into the structural origins of basal heat production. Our composite series of SM prediction equations should prove useful in modeling efforts and in generating hypotheses aimed at understanding how SM relates to body size and shape across the adult lifespan.