Miracle-dz/UniFAR-FTUs huggingface dataset:
Development and Validation of the New Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Unbound Tacrolimus in the Plasma Ultrafiltrate of Transplant Recipients

(1) Background: Only unbound tacrolimus particles are considered to be active and capable of crossing cellular membranes. Thus, the free-drug concentration might be better associated with clinical effects than the total drug concentration used for dosage adjustment. We propose a new, fully validated online liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for unbound tacrolimus concentration measurement. (2) Methods: The determination of the unbound tacrolimus concentration in plasma ultrafiltrate was performed with the Nexera LC system with LCMS-8050 triple quadrupole MS using ascomycin as an internal standard. Chromatographic separation was made using a HypurityC18 analytical column. MS/MS with electrospray ionization and positive-ion multiple-reaction monitoring was used. The unbound tacrolimus level was determined in 36 patients after solid organ transplantation (n = 140). (3) Results: A lower limit of quantification 0.1 pg/mL was achieved, and the assay was linear between 0.1 and 20 pg/mL (R2 = 0.991). No carry-over was detected. The within-run and between-run accuracies ranged between 97.8–109.7% and 98.3–107.1%, while the greatest imprecision was 10.6% and 10.7%, respectively. Free tacrolimus in patients’ plasma ultrafiltrate varied between 0.06 and 18.25 pg/mL (median: 0.98 pg/mL). (4) Conclusions: The proposed method can be easily implemented. The significance of the unbound tacrolimus concentration needs to be investigated. This may facilitate the individualization and optimization of immunosuppressive treatment.
Rethinking Sanctuary: The Origins of Non-Cooperation Policies in Social Welfare Agencies

Too often, scholarship on immigration conflates sanctuary ordinances with the non-cooperation policies, often embedded in these ordinances, which limit cooperation between local officials and federal immigration authorities. In this article, I disentangle the two by tracing the rise of non-cooperation policies in health and welfare agencies since the New Deal. Doing so challenges assumptions about the origins, targets, consequences, and significance of early sanctuary policies. It reveals that non-cooperation was federal policy between 1935 and the early 1970s, when local, state, and federal officials began to experiment with cooperation. When the consequences of such practices became clear, welfare and health officials were forced to reaffirm non-cooperation just before the sanctuary movement burst onto the scene. This research clarifies why scholars see early sanctuary ordinances as largely symbolic: because many local, state, and federal officials had largely abandoned cooperation in practice. It also challenges the widespread assumption that non-cooperation fundamentally represents local resistance to federal power. Instead, I demonstrate the key role played by the federal government in the rise of non-cooperation in health and welfare agencies. Lastly, this research reaffirms the significance of the fragmented nature of federal institutions for promoting immigrant rights.
Efficient Method for Vehicle License Plate Identification Based on Learning a Morphological Feature

The detection and identification of license plates from captured images have been widely studied over the past two decades. The demand for this technology in security and commercial applications ranges from traffic control organisation to parking management and vehicle tracking. License plate recognition can be divided into two steps: detection and identification. Recent algorithms have focused on the detection step, with few researchers studying identification. In this study, an algorithm is developed to detect and identify license plates. The algorithm is based on the fact that license plates have a semi-symmetric distribution of corner points; it utilises morphological feature learning. The algorithm runs in real time, is highly robust, and can identify whether the candidate region contains a license plate.
The Involvement of Sirtuin 1 Dysfunction in High-Fat Diet-Induced Vascular Dysfunction in Mice

High-fat diet (HFD)-induced vascular impairment in mice is associated with a dysfunction of the perivascular adipose tissue (PVAT). The present study was conducted to investigate the involvement of sirtuin 1 (SIRT1). Male C57BL/6J mice were fed an HFD for 20 weeks to induce obesity. Vascular function was analyzed using a wire myograph system. In obese mice, the vasodilator response of PVAT-containing aortas to acetylcholine was reduced, although the vascular function of PVAT-free aortas remained normal. SIRT1 activity in PVAT of obese mice was reduced despite enhanced SIRT1 expression. Nicotinamide adenine dinucleotide (NAD+) levels and the NAD+/NADH ratio in the PVAT of obese mice were decreased, which was likely attributable to a downregulation of the NAD+-producing enzyme NAMPT. The reduced SIRT1 activity was associated with an enhanced acetylation of the endothelial nitric oxide synthase (eNOS) in the PVAT. Ex vivo incubation of PVAT-containing aorta from obese mice with NAD+ led to a complete normalization of vascular function. Thus, reduced SIRT1 activity due to NAD+ deficiency is involved in obesity-induced PVAT dysfunction.
How do internet fraudsters think? A qualitative examination of pro-criminal attitudes and cognitions among internet fraudsters in Nigeria

ABSTRACT As the use of the internet is expanding, crimes committed on the internet are also increasing. The means through which fraudsters carry out their schemes are widely known. The negative impacts arising from the activities of internet fraudsters are felt globally. Yet little is understood about the thinking patterns that allow perpetrators to carry on with such crimes. This may also explain why interventions are scarce. In this study, we interviewed 12 male youngsters who are actively engaged in internet fraud activities (11 in the community and 1 in prison custody) in Nigeria. The aim was to identify pro-criminal attitudes and cognitions that are supportive of perpetrating internet fraud. Thematic analysis was used to unpack findings from the interview transcripts. We found that peers were commonly identified to play a role in introducing youths to the crime. There was also tacit support from communities and families. Pro-criminal cognitions such as neutralization, entitlement, minimization, rationalizations, and dehumanization were found in the narratives of the youngsters. Interestingly, youngsters often rationalized and justified their actions using socio-political/socio-historical antecedents, a position we found was popular in the cybercrime space, especially in Nigeria. Our findings have implications for the designing and implementation of rehabilitation intervention for internet fraudsters.
Impact of Bank Specific Factors on Profitability of Nepalese Commercial Banks

This paper aims to investigate the impact of bank specific factors unprofitability of Nepalese commercial banks. Total assets, total deposit, total loan and advance and total equity are taken as independent variables and return on assets is taken as dependent variable. The study is based on pooled least square method. The seven years’ panel data from 2012 to 2018 is taken for the study. Taking 112 observations from sixteen commercial banks, the regression result, F-test and t-test are used for analysis. The study found that the total assets and total loan and advance have positive significant impact on profitability of Nepalese commercial banks. The study also revealed that the total equity has not significant impact and deposit has negative significant impact on profitability of commercial banks in Nepal. The study has important implications for the policy makers of Nepalese commercial banks to utilize their assets, loans, deposits properly and equity for sound profitability.
Neuroelectric Mechanisms of Delayed Cerebral Ischemia after Aneurysmal Subarachnoid Hemorrhage

Delayed cerebral ischemia (DCI) remains a challenging but very important condition, because DCI is preventable and treatable for improving functional outcomes after aneurysmal subarachnoid hemorrhage (SAH). The pathologies underlying DCI are multifactorial. Classical approaches to DCI focus exclusively on preventing and treating the reduction of blood flow supply. However, recently, glutamate-mediated neuroelectric disruptions, such as excitotoxicity, cortical spreading depolarization and seizures, and epileptiform discharges, have been reported to occur in high frequencies in association with DCI development after SAH. Each of the neuroelectric disruptions can trigger the other, which augments metabolic demand. If increased metabolic demand exceeds the impaired blood supply, the mismatch leads to relative ischemia, resulting in DCI. The neuroelectric disruption also induces inverted vasoconstrictive neurovascular coupling in compromised brain tissues after SAH, causing DCI. Although glutamates and the receptors may play central roles in the development of excitotoxicity, cortical spreading ischemia and epileptic activity-related events, more studies are needed to clarify the pathophysiology and to develop novel therapeutic strategies for preventing or treating neuroelectric disruption-related DCI after SAH. This article reviews the recent advancement in research on neuroelectric disruption after SAH.
Six skin diseases classification using deep convolutional neural network

Smart imaging-based medical classification systems help the human diagnose the diseases and make better decisions about patient health. Recently, computer-aided classification of skin diseases has been a popular research area due to its importance in the early detection of skin diseases. This paper presents at its core, a system that exploits convolutional neural networks to classify color images of skin lesions. It relies on a pre-trained deep convolutional neural network to classify between six skin diseases: acne, athlete’s foot, chickenpox, eczema, skin cancer, and vitiligo. Additionally, we constructed a dataset of 3000 colored images from several online datasets and the Internet. Experimental results are encouraging, where the proposed model achieved an accuracy of 81.75%, which is higher than the state of the art researches in this field. This accuracy was calculated using the holdout method, where 90% of the images were used for training, and 10% of the images were used for out-of-sample accuracy testing.
Textural quality, growth parameters and oxidative responses in Nile tilapia (Oreochromis niloticus) fed faba bean water extract diet

Texture is one of the key quality attributes used in the fresh and processed fish industry to assess product quality and consumer acceptability. To improve the textural quality of tilapia, we formulated the expanded pellet diet (EPD) and pellet diet (PD), both containing faba bean (Vicia faba, FB) water extract, a previously reported potential aquafeed additive to increase flesh texture. The common diet was used as a control. After Nile tilapia (Oreochromis niloticus) were fed three kinds of experimental diet for 120 days, muscle textural quality, growth parameters, oxidative response and immune parameters were analyzed. The results showed that there was no significant difference in the growth parameters between the three groups (P > 0.05). The highest measure of textural quality (hardness and chewiness) was found for the PD group, followed by the EPD and the control (P < 0.05). Less oxidative damage to the hepatopancreas and intestine was found in the EPD compared with the PD group, as demonstrated by the decreased levels of reactive oxygen species and increased levels of nicotinamide adenine dinucleotide and intestinal digestive enzyme activity (amylase and lipase). Taken together, this study highlights the potential usefulness in commercial settings of FB water extract for improving the textural quality of tilapia, and EPD containing faba bean water extract could be more advanced substitute for faba bean in tilapia culture in term of both effectiveness in textural quality improvement and health status enhancement compared with PD.
Association between Alcohol Intake and Arterial Stiffness in Healthy Adults: A Systematic Review

Background: Arterial stiffness as assessed by Pulse Wave Velocity (PWV) represents an independent predictor of cardiovascular disease. Several dietary compounds and lifestyle factors could influence arterial stiffness. The debate on the significance of the correlation between alcohol consumption and arterial stiffness is still open, given that the relationship is complex and potentially affected by several factors such as alcohol type, consumption levels, gender and age differences. Objective: This systematic literature review aims to examine the evidence supporting an association between alcohol use and PWV, in electronic databases including PubMed/MEDLINE and the Cochrane Library, from January 2010 to November 2020. Screening and full-text reviews were performed by three investigators and data extraction by two. Considering the significant heterogeneity of data only a qualitative analysis (systematic review) was performed. Results: A total of 13 studies met the inclusion criteria. Alcohol consumption was independently associated with arterial stiffness in a J-shaped way in most of the studies included. A benefit of alcohol consumption on arterial stiffness was found in four experimental studies, whilst an unfavorable increasing linear association was found in four others. Associations were confirmed with both oscillometric and tonometric PWV assessment methods. In some studies, a gender and age correlation was found with a more pronounced association in older males. In all studies elevated levels of alcohol consumption were associated with a worsening of arterial stiffness. Conclusions: Despite the variable findings across studies, the current review provides preliminary evidence that light-to-moderate alcohol consumption is associated with arterial stiffness values lower than expected, and evidence that high doses accelerate arterial ageing. These findings could be useful for clinicians who provide recommendations for patients at cardiovascular (CV) risk. Nevertheless, given the heterogeneity of study designs, interventions, measurement methods and statistical evaluations, the protective role of moderate alcohol consumption on arterial stiffness is likely but not certain, warranting additional trials and evidence.
A New Modeling Approach for Predicting Vehicle-Based Safety Threats

Existing autonomous driving systems of intelligent vehicles such as advanced driver assistant systems (ADAS) assess and quantify the level of potential safety threats. However, they may not be able to plan the best response to unexpected dangerous situations and do not have the ability to cope with uncertainties since not all vehicles can always keep a safe gap from preceding vehicles and drive at a desired velocity. Previous research has not taken such uncertainties into account, it is, therefore, necessary to develop models which are not restricted by the predefined movement patterns of a vehicle. Existing systems are based on a model that estimates the threat level based only on one factor Time-To-Collision (TTC). This approach is limited since it cannot handle all scenarios and ignores all uncertainties. To overcome these limitations, this paper utilised deep learning to develop a range of models that rely on a group of factors to reliably estimate the threat level and predict conflicts under uncertainty using the concept of looming ’. Comparative analyses were undertaken by incorporating new varying input factors to each model (e.g., surrogate safety measures, vehicle kinematics, macroscopic traffic data). Real-world experiments demonstrated that adding new factors increases the reliability and sensitivity of the models. Results also indicated that the models that consider looming provide low false alarm rate extending their applications for a wider spectrum of traffic scenarios. This is paramount for ADAS as uncertainties are inherent in the deployment of connected and autonomous vehicles in a mixed traffic stream.
The Novel Role of Cytomorphology from Ultrasound-Guided Fine Needle Aspiration Cytology in Evaluating the Status of Prognostic Factors including Estrogen Receptor, Progesterone Receptor and HER2 in Breast Cancer

It is well established that estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor (HER2) could be regarded as prognostic factors in breast cancer. Ultrasound-guided fine needle aspiration cytology (FNAC) has revolutionized the management of cancers, providing less invasive and quick diagnostic method. There are hardly any studies on the correlation between cytomorphology and prognostic biomarkers. We retrospectively analyzed the immunohistochemistry and the fluorescence in situ hybridization of breast cancer specimens from 252 patients, who have been diagnosed as breast cancer at our hospital. Morphological features of cytology smears were scored. The relationship between cytological features and three biomarkers were analyzed. Based on this, we developed a system to predict the status of biomarkers. The results indicated that some cytological parameters, especially the features of nucleoli, were distinctively related to the makers' expression. In the novel scoring system, a cutoff of 12.0 provided a statistical discrimination for cytological grading. We concluded that cytomorphological features were associated with prognostic factors. The HR+ neoplasms showed scattered micronucleoli, while HER2+ neoplasms demonstrated centered macronucleoli. We summarized a scoring system to predict the status of three factors. This may help us to broaden the application of breast cancer cytology.
A Fast Approach for SAR Polarimetric Computations of Entropy/Alpha Angles

We present an approach based on parallel computing on graphics processing units using the CUDA language for the evaluation of the Cloude and Pottier decomposition. The computation of eigenvalues and eigenvectors of the 3 3 3 covariance matrix is worked out by exploiting its Hermitian property by a ‘‘Cardano-like,’’ direct analytical scheme. Numerical results show the benefits of the approach.
Effect of Korean Dental Hygienists’ Experiences Related to the Elderly on Their Perception of Human Rights Violations of the Elderly: A Cross-Sectional Study

Background: Korean society has become an elderly society at an unprecedented rate, and the associated social and economic problems are very serious. Maintaining a healthy oral condition is important for older people’s well-being and quality of life, and is essential for healthy ageing. Therefore, the purpose of this study was to investigate the effect of dental hygienists’ experiences related to the elderly on their perception of human rights violations toward the elderly. Methods: This study was conducted through an IRB review at Silla University in Korea (No. 1041449-202012-HR-001). For about a month after 1 February 2021, the survey questionnaire was distributed to dental hygienists living in South Korea, and 153 people who completed and sent back the questionnaires were selected as the final subjects. The items on the questionnaire consisted of perception of human rights violations for the elderly and experiences related to the elderly. A polyserial correlation analysis was performed to confirm the relevance of each factor, and a multiple regression analysis was performed to identify the factors of elderly-related experiences that affect the perception of human rights violations against the elderly. Results: All three experiences related to the elderly (the experience of receiving education related to the elderly, the experience of volunteer work related to the elderly, and the experience of being interested in issues related to the elderly) were highly related to the perception of violations of financial human rights of the elderly (p < 0.01). In addition, the experience of education related to the elderly showed a high perception of psychological violations of the human rights of the elderly (R = 0.405, p < 0.01), and the experience of volunteer activities related to the elderly showed a high perception of neglectful violations of human rights of the elderly (R = 0.277, p < 0.01). Conclusions: In this study, it was confirmed that there is a relationship between dental hygienists’ experiences related to the elderly and their perception of human rights violations in the elderly. Therefore, dental hygienists should do their best to manage the elderly’s oral health by raising awareness of the human rights violations against the elderly through the elderly professional course based on their experience with the elderly.
Role of CCR2-Positive Macrophages in Pathological Ventricular Remodelling

Even with recent advances in care, heart failure remains a major cause of morbidity and mortality, which urgently needs new treatments. One of the major antecedents of heart failure is pathological ventricular remodelling, the abnormal change in the size, shape, function or composition of the cardiac ventricles in response to load or injury. Accumulating immune cell subpopulations contribute to the change in cardiac cellular composition that occurs during ventricular remodelling, and these immune cells can facilitate heart failure development. Among cardiac immune cell subpopulations, macrophages that are recognized by their transcriptional or cell-surface expression of the chemokine receptor C-C chemokine receptor type 2 (CCR2), have emerged as playing an especially important role in adverse remodelling. Here, we assimilate the literature that has been generated over the past two decades describing the pathological roles that CCR2+ macrophages play in ventricular remodelling. The goal is to facilitate research and innovation efforts in heart failure therapeutics by drawing attention to the importance of studying the manner by which CCR2+ macrophages mediate their deleterious effects.
Compact ultra-wide stopband low pass filter using multimode resonators

A compact microstrip ultra-wide stopband low pass filter using multimode resonators is presented. It consists of a narrow width microstrip line with high impedance and three multimode resonators. The characteristics and equivalent circuits of the multimode resonator are investigated. Owing to the strong slow-wave and bandstop effects, the multimode resonator can be implemented to achieve an ultra-wide stopband and size reduction. At the 3 dB cut off frequency of 1.8 GHz, the measured stopband of the low pass filter with 15 dB suppression is from 2.83 to 24 GHz. The physical size of the proposed low pass filter is 0. 09λ g × 0. 11λ g. The presented simulation and measurement results are in good agreement.