Miracle-dz/UniFAR-FTUs huggingface dataset:
The transcriptomic profile of CD138+ cells from patients with early progression from smoldering to active multiple myeloma remains substantially unchanged

Smoldering myeloma (SMM) is a pre-malignant monoclonal gammopathy with an annual risk of about 10% of progressing to active multiple myeloma (MM). The International Myeloma Working Group (IMWG) has recently updated the diagnostic criteria for SMM. The previously defined “ultra high-risk” SMM (characterized by specific biomarkers associated with a greater than 80% risk of progression to symptomatic MM within 2 years) has now been incorporated in active MM. SMM is biologically heterogeneous and encompasses a condition with a very low rate of progression to symptomatic MM, behaving similarly to monoclonal gammopathy of uncertain significance, as well as a disease with acquired organ damage and progression to active MM within 5 years from diagnosis.
PCR based Detection of Helicobacter spp. in Saliva, Dental plaque, Vomitus and Feces of Dogs

This study was aimed to determine the prevalence of Helicobacter spp. in privately owned pet dog’s oral cavity samples (saliva, dental plaque, vomitus) and fecal samples in Korea and to evaluate the potential route for transmission. Total 100 patients dogs attending one Veterinary Medical Teaching Hospital were examined by Helicobacter genus-specific PCR assay and these dogs were divided into two groups whether they had gastrointestinal signs (vomiting, nausea and diarrhea) or not. The total detection rate of Helicobacter spp. by PCR in saliva, dental plaque and fecal samples was 23%, 1% and 68% respectively. The difference of prevalence with regarding the gastrointestinal sings was not significant. In vomitus, two of seven samples had positive results. These results suggested that Helicobacter spp. are present in the oral cavity although they were present in very low number and are not like to be normal oral flora of the oral cavity and Helicobacter spp. in dogs could be transmitted through oral-oral, gastrooral and fecal-oral route.
bcl-2 Gene hypomethylation and high-level expression in B-cell chronic lymphocytic leukemia

The bcl-2 gene becomes transcriptionally deregulated in the majority of low-grade non-Hodgkin lymphomas as a result of t(14;18) translocations that place the bcl-2 gene at 18q21 into juxtaposition with the Ig heavy-chain locus at 14q32. This chromosomal translocation or similar bcl-2 gene rearrangements involving the Ig light-chain genes have been reported to occur in some cases of B-cell chronic lymphocytic leukemia (B-CLL). We analyzed the structure, methylation, and expression of the bcl-2 gene in 20 cases of B-CLL or closely related variants of this lymphoproliferative disorder, including at least 16 typical examples of CD5+ B-CLL. None of the 20 specimens had evidence of bcl-2 gene rearrangements, based on Southern blot analysis using three different bcl-2 probes. However, immunoblot analysis using antibodies specific for the Bcl-2 protein showed that 14 of 20 cases (70%) contained levels of p26-Bcl-2 that were equal to or greater than those found in a t(14;18)-bearing lymphoma cell line. Furthermore, in 19 of 20 cases (95%), the Bcl-2 protein was present at levels that were 1.7- to 25-fold higher than in normal peripheral blood lymphocytes. These differences in the relative levels of Bcl-2 protein among cases of B-CLL appeared to be functionally significant, in that a preliminary analysis of 3 representative cases showed that CLL cells with higher levels of Bcl-2 protein survived longer in culture and were delayed in their onset of DNA degradation relative to CLL cells with lower Bcl-2 protein levels. Evaluation of the methylation status of the bcl-2 gene using the isoschizomers Msp I and Hpa II, and a probe corresponding to the first major exon of the gene showed complete demethylation of both copies of the bcl-2 gene in a region corresponding to a 2.4-kb Msp I fragment in all 20 cases of B-CLL. In contrast, analysis of 6 of 6 B-cell lines that harbor a t(14;18) was consistent with hypomethylation of only one of the two bcl-2 alleles. Neither copy of the bcl-2 gene was demethylated in this region in 5 of 5 lymphoid cell lines that lack this translocation. However, hypomethylation of the bcl-2 gene did not necessarily correlate with the relative levels of Bcl-2 protein present in the B-CLL cells, suggesting that additional mechanisms for regulating bcl-2 expression are involved. (ABSTRACT TRUNCATED AT 400 WORDS)
Complete Deficiency of Glycophorin A in Red Blood Cells From Mice With Targeted Inactivation of the Band 3 (AE1) Gene

Glycophorin A is the major transmembrane sialoglycoprotein of red blood cells. It has been shown to contribute to the expression of the MN and Wright blood group antigens, to act as a receptor for the malaria parasite Plasmodium falciparum and Sendai virus, and along with the anion transporter, band 3, may contribute to the mechanical properties of the red blood cell membrane. Several lines of evidence suggest a close interaction between glycophorin A and band 3 during their biosynthesis. Recently, we have generated mice where the band 3 expression was completely eliminated by selective inactivation of the AE1 anion exchanger gene, thus allowing us to study the effect of band 3 on the expression of red blood cell membrane proteins. In this report, we show that the band 3 -/- red blood cells contain protein 4.1, adducin, dematin, p55, and glycophorin C. In contrast, the band 3 -/- red blood cells are completely devoid of glycophorin A (GPA), as assessed by Western blot and immunocytochemistry techniques, whereas the polymerase chain reaction (PCR) confirmed the presence of GPA mRNA. Pulse-label and pulse-chase experiments show that GPA is not incorporated in the membrane and is rapidly degraded in the cytoplasm. Based on these findings and other published evidence, we propose that band 3 plays a chaperone-like role, which is necessary for the recruitment of GPA to the red blood cell plasma membrane.
Opioid use disorder in a 5-year-old child: A case report from Western Rajasthan

Opioid use disorder is very common in adolescents and adults, but is rarely reported in preschool and school going children. We hereby describe a 5-year-old boy, with history of opioid use who presented with insomnia, irritability, abdominal cramps, body aches, yawning, and running nose, and was later successfully managed by a multimodal approach.
HYPOGLYCEMIC AND HYPOCHOLESTEROLEMIC EFFECTS OF ACACIA TORTILIS (FABACEAE) GROWING IN MAKKAH

Traditional use of herbs and plants is centuries old and they have shown therapeutic properties. But scientific studies are not done to confirm their efficacy and margin of safety. Acacia was collected from UQU, Makkah. Leaves were separated from shoots and aqueous extract of Acacia tortilis was prepared at the concentration of 100mg/ml. Acacia aqueous extract was administered at the dose of 800mg/kg in treated rats for seven days. The various parameters studied include blood glucose levels, serum cholesterol levels, HDL, LDL, AST, ALT and Urea. All parameters were estimated using diagnostic kits spectrophotometrically. At the end of study autopsy also performed. Administration of Acacia aqueous extract (800mg/kg) for seven consecutive days caused significant (P< 0.05) decrease in blood glucose, serum total cholesterol, and LDL level while an increase in serum HDL- cholesterol was observed as compared to their control rats. However a statistically significant (P< 0.05) decrease in body weight was also observed. Autopsy did not show any sign of abnormality and all rats were comparable with their control rats. The findings of the present study confirm that above-mentioned plant validates our claim. In the light of our pharmacological and toxicological studies, Acacia tortilis appears to be a valuable plant, which can be useful, at least as an adjunct, in the therapy of diabetes and hypercholesterolemia or in a condition in which hyperglycemia and dyslipidemia coexist quite often. Further the toxicological, hematological and autopsy results further confirm the wide marginal safety of Acacia tortilis leaves aqueous extract.
Are doctors part of the problem

Many doctors believe that the maternity services they provide are the key to achieving safe motherhood. With emergency facilities immediately available they know that a complicated delivery is far less likely to result in maternal death. Most medical experts therefore tend to assume that more maternity beds means less maternal mortality. That assumption however may not be valid. Many women in Africa and elsewhere choose not to use available maternity facilities because they dislike the services provided by hospital physicians. Examples of womens aversion to hospital delivery are briefly presented from Guinea Bissau the Philippines and Ecuador. Women may not want to be attended by a male physician have fear of being asked to give birth lying down rather than in a squatting position and/or wish to avoid the rude and callous treatment received in hospital. Anthropology researchers working in Bolivia recommended regular discussion sessions between mothers-to-be and the hospital and health service staff to help make hospital services more acceptable to pregnant women without adversely affecting the quality of medical care.
The fitness to work certificate in a worker exposed to ionizing radiation with an oncological disease: criteria and assessment process

To assess the fitness to work certificate in employees both affected by neoplasia and exposed to ionizing radiation always represents a clinical and professional issue, as well as human and social. The aim of our study is to analyze the case of a 40-year-old clinician (woman, endoscopist, gastroenterologist) who underwent left mastectomy and breast reconstruction with a tissue expander, professionally exposed to ionizing radiation. Due to the presence of the BRCA1 gene mutation and the presence of breast and ovarian cancer familiarity, the patient also underwent a prophylactic right mastectomy and bilateral oophorectomy. According to Italian legislation and international guidelines, several elements and criteria have been taken into account to release the fitness to work certificate in this peculiar case of a female worker exposed to ionizing radiation, resulting in a final positive indication.
Waist Circumference and the Relation to Aerobic Exercise and Perception of Illness in Adults with Type 2 Diabetes Mellitus

The purpose of this study was to observe the relationship between waist circumference (WC), amount of aerobic exercise performed weekly and overall Health Belief Model (HBM) score in adults with diagnosed type 2 diabetes mellitus (T2DM). Members from a local Midwest adult fitness program (n = 5) who had been diagnosed T2DM participated in this study. Participants ranged in age from 70 to 80 years with an average age of 74 years. Four of the participants were female and one participant was male. The questions included in this study were 1 . ) in those with diagnosed T2DM, was a low overall HBM score correlated with a high WC? and 2. ) was there a relationship between the overall HBM score and whether individuals with diagnosed T2DM met American College of Sports Medicine's (ACSM's) guidelines for aerobic exercise? WC measurements were taken by the primary researcher and the type and amount of aerobic exercise participants completed was documented as part of the demographic survey. Participants' perception of i llness was analyzed using a version of the Health Belief Model, adapted from Gutierrez and Long (201 1 ). Analysis of the data was done by first calculating Cronbach's alpha to assess internal reliability of the HBM used. Both a Pearson's Correlation Coefficient r and a p-value were then calculated to test the first hypothesis, yielding values ofr = 0.09 and p = 0.89, respectively. These results both showed a non-significant relationship between WC measurement and the overall HBM score in this sample of adults with diagnosed T2DM. Similarly, the second hypothesis was assessed by both a Pearson's Correlation Coefficient r and a p-value, yielding values of r = 0.36 and p = 0.55, respectively. These results showed a non-significant relationship between the overal l HBM score and whether participants met ACSM's guidelines for WAIST CIRCUMFERENCE, EXERCISE, ILLNESS PERCEPTION 3 aerobic exercise. Overall, the results of this study did not support either hypothesis, indicating no observed relationship between WC measurements and overall HBM score or between overall HBM score and whether individuals met ACSM's guidelines for aerobic exercise. It is recommended that future studies use a larger population in order to obtain a larger sample size, hopefully yielding more significant results. Additionally, it is recommended that future studies sample from multiple facilities and more diverse populations in order to increase the variation in age range and duration of diagnosis
The relationship of financial performance, quality indicators, and the role of nurse executives in Virginia nursing homes

THE RELATIONSHIP OF FINANCIAL PERFORMANCE, QUALITY INDICATORS, AND THE ROLE OF NURSE EXECUTIVES IN VIRGINIA NURSING HOMES Jeany V. Murphy Old Dominion University, 2011 Director: Dr. Karen Karlowicz Problem statement. The dynamic nature of the healthcare industry has triggered changes in nurse executive functions. The job has expanded into entrepreneurial roles to be able to provide the most cost effective services while maintaining good quality outcomes for the residents. The new responsibility requires a different set of knowledge and competencies that is usually achieved through advanced education. It is not known whether nurse executives in long term care in Virginia possess the skills to influence both the financial and quality outcomes of the facility. Methods. This descriptive research study examined the relationship of the nurse executives' technical, human, and conceptual skills and levels of education to the facilities' financial performance and resident outcomes. Further, this study compared the perceptions of chief executive officers (CEO) and nurse executives regarding the skills required of nurse executives to be effective managers. A combination of web-based and mail surveys were sent to all CEOs and nurse executives of Virginia nursing homes. Ninety-eight CEOs and nurse executives (18.4%) participated in the study. Results. There were no statistically significant differences between CEOs' and nurse executives' perception of the importance of knowledge and competencies related to human, technical, and conceptual skills of nurse executives for effective management. Both CEOs and nurse executives perceived human skills as the most important expertise for effective management. Technical knowledge (p<.05) of nurse executives and total number of residents (p<.001) showed significant relationship with the facility's financial performance. No variables predicted quality outcomes. Conclusion. The nurse executives' level of preparedness is minimal. The small percentage of graduate prepared nurse executives coupled with what appears to be limited influence in the organization is not adequate to impact the financial performance and quality outcomes of the facilities.
The Status of Iodine Deficiency in Mehrauli area of South Delhi

Background: Iodine deficiency disorders (IDDs) are important public health problem affectingalmost the entire country. It means not only goiter but all related disorders of physical growthand brain development. The literature on the magnitude of Iodine deficiency amongchildren in Delhi is limited. Present community based study was conducted to obtain better knowledge on Iodine deficiency in Delhi.Objective: To find out the prevalence of goiter and iodine level in urine and household salt among children of 6-12 years in Mehrauli area of Delhi.Methodology: Both boys and girls of age 6-12 years were selected from all the eight existing wards of Mehrauli, Delhi. A total of 1456 children were examined for goiter . Urine samples for estimating iodine excretion level were collected from 146 children and 292 household salt samples were collected for estimating iodine level in salt. The study subjects were spread over 1016 households.Results: In the study, total goiter rate (TGR) was found to be 1.7% (n=25) which is below the level of WHO defined public health problem. Median urinary iodine excretion level was found to be 248. 5µg/l in overall 146 urinary samples ruling out iodine deficiency in the area. Excess iodine (above acceptable levels i.e. >200µg/l) was found to be present in 91 samples (62.3%). However iodine content was found to be adequate in 271 (93%) salt samples. Mean iodine content in salt was found to be 35.7±11.1 ppm.Discussion and conclusion: The urinary and salt iodine content in the study area are above the recommended cut off suggested by WHO/ICCIDD/UNICEF for defining of iodine deficiency disorders. Thus iodine deficiency is no longer a public health problem in the study area. However there is a need for periodic monitoring of salt samples as about 7% of households still consumed salt of below 15 ppm of iodine. Community based awareness campaign may be an effective strategy to motivate more people for consuming iodised salt and knowing about the dangers of iodine deficiency .
Comparison of Complications Following Implant-Based Breast Reconstruction Using Triple Antibiotic Solution versus Low Concentration Chlorhexidine Gluconate Solution

Background: Prevention of infection and capsular contracture remains a primary goal of implant-based breast reconstruction (IBBR). Previous studies have demonstrated improved outcomes with the use of triple-antibiotic solution (TAS) for breast pocket irrigation, but ready-to-use products have recently gained popularity. The purpose of this study is to compare outcomes following IBBR between TAS and low-concentration chlorhexidine gluconate (CHG) solutions. Methods: This is a retrospective analysis of 690 consecutive patients undergoing IBBR from 2008-2017. The TAS (n = 346) irrigation solution was composed of 1 g cefazolin, 80 mg gentamicin and 50,000 U bacitracin diluted in 500 mL of normal saline; the CHG (n = 344) irrigation solution was the commercially-available product Irrisept (0.05% chlorhexidine gluconate in sterile water, Irrimax Corporation, Lawrenceville, GA). Comparisons were made between demographic and clinical variables. Complications were recorded and statistical analysis, including multivariate regression analysis, was performed. Results: The TAS group underwent significantly more skin-sparing mastectomies, adjuvant chemotherapy/radiation and less direct-to-implant reconstruction than the CHG group. The CHG group experienced a significantly lower incidence of total complications (22.4% vs. 31.8%, p = 0.006), minor complications (8.7% vs. 16.5%, p = 0.003), infection (6.4% vs. 12.7%, p = 0.006) and seroma (2.6% vs. 6.9%, p = 0.011). There was a significantly increased rate of delayed wound healing in the CHG group. Multivariate analysis showed that the use of CHG solution significantly decreased the odds of any complication by 1.6-fold (OR 0.637, 95% CI 0.414 - 0.977) and the odds of infection by 2.4-fold (OR 0.420, 95% CI 0.218 - 0.809). There were no statistically significant differences in rates of capsular contracture or other complications. Conclusions: The use of CHG as a pocket irrigant in post-mastectomy breast reconstruction is a reasonable alternative to other solutions, in efforts to minimize prosthetic based complications.
Risk factors in infra-inguinal graft stenosis

Intimai hyperplasia appears to be the universal vessel response to injury and is important in atherogenesis. In animal and in-vitro models, the initiation and promotion of this process has been associated with a number of risk factors found in the circulation. In man, approximately 25% of infrainguinal bypass grafts develop stenosis due to smooth muscle cell proliferation but it is not known if circulating risk factors influence this process. In these studies, circulating risk factors have been correlated with the development of hyperplastic stenoses in infrainguinal bypass grafts. Non-hyperplastic causes of stenosis have been excluded by peri operative graft assessment. Duplex scanning and angiography were used to detect new stenoses oceuring in the first postoperative year. Logistic regression shows association between graft stenoses and; continued cigarette smoking and elevated circulating levels of fibrinogen, Lp(a) and 5-HT. These associations are independent of graft material or whether grafts were studied prospectively or retrospectively. There were some differences in stenosis associated risk factors between vein and PTFE graft groups on univariate analysis. These results suggest that circulating risk factors do play a role in intimai hyperplasia and stenosis of human infrainguinal bypass grafts and support other work demonstrating a reduction in graft patency in association with similar abnormalities.
Administration of Misoprostol Sublingual versus Vaginal for the Termination of Second Trimester Missed Miscarriage

Accept Date 2019-10-08 ABSTRACT Background: miscarriage is the process of fetus extraction (embryo) weighting less than 500 mg equivalent to approximately 20 to 22 weeks gestation. Misoprostol is increasingly used for second trimester termination of pregnancy. It is inexpensive, and it is rapidly absorbed by sublingual, vaginal and oral routes. Objectives: This study was carried out for comparing the safety of misoprostol administrated sublingually versus vaginally in the middle of trimester missed miscarriage termination without surgical intervention and with minimum complication. Patients & Methods: The study was carried out in high risk unit of Zagazig university and in Whada Derna teaching hospital during the period from April 2017 to January 2018, 32 pregnant women diagnosed as missed miscarriage were divided to 2 groups, Group A: Received a dose of 200μg of Misoprostol sublingual and Group B: Received a dose of 200μg of Misoprostol vaginally, both each 4 hours up to 6 doses a day. Results: there was a high statistical significant differences the two groups according to the means of induction to miscarriage interval. 15 patients in sublingual group and 7 patients in vaginal group miscarriage during 12 hours after treatment. Conclusion: Sublingual and vaginal administration of 200 microgram of misoprostol each 4 hours up to 6 doses a day is effective in the second trimester termination in missed miscarriage, sublingual administration is the best choice due to its high response, acceptability, less side effects and great effect, sublingual misoprostol can be selfadministrated by patients at home thereby decreasing hospital stay and costs.
Learning Interpretable Negation Rules via Weak Supervision at Document Level: A Reinforcement Learning Approach

Negation scope detection is widely performed as a supervised learning task which relies upon negation labels at word level. This suffers from two key drawbacks: (1) such granular annotations are costly and (2) highly subjective, since, due to the absence of explicit linguistic resolution rules, human annotators often disagree in the perceived negation scopes. To the best of our knowledge, our work presents the first approach that eliminates the need for world-level negation labels, replacing it instead with document-level sentiment annotations. For this, we present a novel strategy for learning fully interpretable negation rules via weak supervision: we apply reinforcement learning to find a policy that reconstructs negation rules from sentiment predictions at document level. Our experiments demonstrate that our approach for weak supervision can effectively learn negation rules. Furthermore, an out-of-sample evaluation via sentiment analysis reveals consistent improvements (of up to 4.66%) over both a sentiment analysis with (i) no negation handling and (ii) the use of word-level annotations from humans. Moreover, the inferred negation rules are fully interpretable.