Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of Adenovirus with the Microtubule Organizing Center

Adenoviruses (Ad) must deliver their genomes to the nucleus of the target cell to initiate an infection. Following entry into the cell and escape from the endosome, Ad trafﬁcs along the microtubule cytoskeleton toward the nucleus. In the ﬁnal step in Ad trafﬁcking, Ad must leave the microtubule and establish an association with the nuclear envelope. We hypothesized that in cells lacking a nucleus, the capsid moves to and associates with the microtubule organizing center (MTOC). To test this hypothesis, we established an exper- imental system to examine Ad trafﬁcking in enucleated cells compared to Ad trafﬁcking in intact, mock-enucleated cells. Enucleation of a monolayer of A549 human lung epithelial cells was accomplished by depolymerization of the actin cytoskeleton followed by centrifugation. Upon infection of enucleated cells with Cy3-labeled Ad, the majority of Ad capsid trafﬁcked to a discrete, centrally located site which colocalized with pericentrin, a component of the MTOC. MTOC-associated Ad had escaped from endosomes and thus had direct access to MTOC components. Ad localization at this site was sensitive to the microtubule-depolymer- izing agent nocodazole, but not to the microﬁlament-depolymerizing agent cytochalasin B, indicating that intact microtubules were required to maintain the localization with the MTOC.
Factors influencing online shopping behavior of University students in Hanoi, Vietnam: A model and empirical study

: By adopting selectively two classical models of consumer behavior, Theory of Rational Action and Technology Acceptance Model, and combining with two other factors, the paper proposed a model of six factors including Attitude, Perceived Risk, Perceived Usefulness, Perceived Ease of Use, Subjective Norm and Web Quality to assess determinants of university students’ online shopping behavior in Hanoi, Vietnam. The model was assessed through Explanatory Factor Analysis, regression analysis and independent sample test based on data collected from 289 university students in Hanoi, Vietnam. The results showed that Perceived Usefulness plays the most important role in determining students’ online shopping behavior, followed by Web Quality and Attitude. There is also a difference in online shopping behavior between the third-year and first-year students, and between the third-year and second-year students. From these results, the paper drew out implications to support E-commerce enterprises to attract more university students to shop online, and strengthen the development of E-commerce in Vietnam
TEACHER JOB STRESS IN ALBANIA: EXAMINING THE ROLE OF STUDENTS’ CLASSROOM DISRUPTIVE BEHAVIOR AND OTHER FACTORS IN THE SCHOOL CONTEXT

The purpose of this study was to determine the impact of the students’ disruptive behavior and other factors in the school context on the teachers’ job stress. For this purpose a survey based descriptive research was conducted with 540 basic education teachers. The instrument used was a structured questionnaire which was comprised of four scales for measuring teacher stress, students’ disruptive behavior, time pressure and workload, relations with school principal and relations with colleagues. Pearson product-moment correlation coefficient was used to determine the direction and the strength of the relationships between the teachers’ job stress and the stress related factors. Standard multiple regression was used to determine the amount of variability on teachers’ job stress explained by the independent factors involved in the study. The study findings indicated low to substantial correlations between teachers’ job stress, students’ disruptive behaviour, time pressure and workload, relations with school principal and relations with colleagues. Students’ disruptive behaviour, time pressure and workload and relations with school principal were found to be significant predictors of teachers’ job stress. Relations with colleagues were not found to be a significant predictor of teacher stress. Key words: relation with colleagues, relation with school principal, students’ disruptive behavior, teacher job stress, time pressure and workload.
EVALUATION OF BEHAVIOR AND WELFARE IN BROILERS REARED IN TWO DIFFERENT HOUSING SYSTEMS

ABSTARCT An experiment was conducted to evaluate the effect of different housing system (cages vs. floor system) on behavioral patterns and welfare of broiler chicks. One hundred and twenty broiler were allocated in to two housing system (cages and floor pen) in full environmental controlled pens for four weeks. Assessment of welfare was through measuring general behavioral, tonic immobility test and heterophil: lymphocyte ratio (HLR). Compared to cage reared birds, floor reared broilers exhibit higher level of feeding, drinking, crouching, walking behavior while caged birds were more often standing and also showed either more wing stretch, leg stretch or both wing& leg stretch than floor reared group. Moreover, shortest duration of tonic immobility test was observed in floor reared birds than those housed in cages. HLR were significantly higher in cages than floor group. At the end of this experiment our obtained data suggested that housing in cages has adverse effect on welfare as it impair general behavioral patterns, birds showed more fearfulness also higher HLR which over all indicated stressful conditions for birds. In conclusion, cages should not be recommended for broiler rearing from welfare point of view.
Explorer The influence of plant species, tissue type and temperature on the capacity of Shigatoxigenic to colonise, grow and internalise into plants

Contamination of fresh produce with pathogenic Escherichia coli, including Shigatoxigenic E. 2 coli (STEC), represents a serious risk to human health. Colonisation is governed by multiple 3 bacterial and plant factors that can impact the probability and suitability of bacterial growth. Thus, we aimed to determine whether the growth potential of STEC for plants associated with 5 foodborne outbreaks (two leafy vegetables and two sprouted seed species), is predictive for colonisation of living plants as assessed from growth kinetics and biofilm formation in plant 7 extracts. Fitness of STEC was compared to environmental E. coli , at temperatures relevant to 8 plant growth. Growth kinetics in plant extracts varied in a plant-dependent and isolate- 9 dependent manner for all isolates, with spinach leaf lysates supporting the fastest rates of 10 growth. Spinach extracts also supported the highest levels of biofilm formation. Saccharides 11 were identified as the major driver of bacterial growth, although no single metabolite could be 12 correlated with growth kinetics. The highest level of in planta colonisation occurred on alfalfa 13 sprouts, though internalisation was 10-times more prevalent in the leafy vegetables than in 14 sprouted seeds. Marked differences in in planta growth meant that growth potential could only 15 be inferred for STEC for sprouted seeds. In contrast, biofilm formation in extracts related to 16 spinach colonisation. Overall, the capacity of E. coli to colonise, grow and internalise within 17 plants or plant-derived matrices were influenced by the isolate type, plant species, plant tissue 18 type and temperature, complicating any straight-forward relationship between in vitro and in 19 planta behaviours. transformed into isolate H110320350 allelic delete stx1 and stx2 sequentially, PCR primers: stx1 (5’-ATAAATCGCCATTCGTTGACTAC and 5’-AGAACGCCCACTGAGATCATC) and stx2 (5’-GGCACTGTCTGAAACTGCTCC and 5’-TCGCCAGTTATCTGACATTCTG).
Priority Based Dynamic Round Robin with Intelligent Time Slice and Highest Response Ratio Next Algorithm for Soft Real Time System

:- In this paper, we have improved the work of Round Robin (RR) algorithm to make it suitable for Soft Real-Time system and that because Round Robin (RR) algorithm suitable for timeshared systems but it is not suitable for Soft Real-Time system due to a large waiting time , large number of context switch and large response time, for this we have improved it by proposing a new algorithm to improve the concept of Dynamic quantum time in [3], by take the Highest Response Ratio Next (HRRN) for each process in each round to select the next process from ready queue , because one of the most important challenges in scheduling is how choose the next process for execute, where we found that the proposed algorithm gives better results than in [3] in terms of reducing the number of context switches, average waiting time and average turnaround time.
Handgrip strength of community-dwelling elderly in Nakhon Ratchasima Province, Thailand

Objective To investigate handgrip strength (HGS) in Th ai elderly living in Nakhon Ratchasima Province, Th ailand, and to examine factors related to HGS. Methods In this is a cross-sectional analytical study, participants were elderly individuals (age >60 years) living in Nakhon Ratchasima, Th ailand. Demographic characteristics, anthropometric measurements, and Barthel Activities of Daily Living index (ADL) were recorded. Assessment of grip strength of the dominant hand was conducted following standardized testing procedures of the American Society of Hand Th erapists. Results Th e mean age (±SD) of the 491 participants was 69.15±6.86 years. Th e mean HGS for male participants was signifi cantly higher than for females (24.93±7.45 vs 16.17±4.82, p <0.001), but there was a signifi cant decline in HGS with age in both genders (r=-0.310, p <0.01). Dividing participants by BMI found that normal and overweight individuals had a higher HGS than underweight participants ( p <0.001).
University of Birmingham Association of non-alcoholic fatty liver disease with chronic kidney disease: a systematic review and meta-analysis

Background: Chronic kidney disease (CKD) is a frequent, under-recognized condition and a risk factor for renal failure and cardiovascular disease. Increasing evidence connects non-alcoholic fatty liver disease (NAFLD) to CKD. We conducted a meta-analysis to determine whether the presence and severity of NAFLD are associated with the presence and severity of CKD. MethodsandFindings: English and non-English articles from international online databases from 1980 through January 31, 2014 were searched. Observational studies assessing NAFLD by histology, imaging, or biochemistry and defining CKD as either estimated glomerular filtration rate (eGFR) , 60 ml/min/1.73 m 2 or proteinuria were included. Two reviewers extracted studies independently and in duplicate. Individual participant data (IPD) were solicited from all selected studies. Studies providing IPD were combined with studies providing only aggregate data with the two-stage method. Main outcomes were pooled using random-effects models. Sensitivity and subgroup analyses were used to explore sources of heterogeneity and the effect of potential confounders. The influences of age, whole-body/abdominal obesity, homeostasis model of insulin resistance (HOMA-IR), and duration of follow-up on effect estimates were assessed by meta-regression. Thirty-three studies (63,902 participants, 16 population-based and 17 hospital-based, 20 cross-sectional, and 13 longitudinal) were included. For 20 studies (61% of included studies, 11 cross-sectional and nine longitudinal, 29,282 participants), we obtained IPD. NAFLD was associated with an increased risk of prevalent (odds ratio [OR] 2.12, 95% CI 1.69– 2.66) and incident (hazard ratio [HR] 1.79, 95% CI 1.65–1.95) CKD. Non-alcoholic steatohepatitis (NASH) was associated with a higher prevalence (OR 2.53, 95% CI 1.58–4.05) and incidence (HR 2.12, 95% CI 1.42–3.17) of CKD than simple steatosis. Advanced fibrosis was associated with a higher prevalence (OR 5.20, 95% CI 3.14–8.61) and incidence (HR 3.29, 95% CI 2.30– 4.71) of CKD than non-advanced fibrosis. In all analyses, the magnitude and direction of effects remained unaffected by diabetes status, after adjustment for other risk factors, and in other subgroup and meta-regression analyses. In cross- sectional and longitudinal studies, the severity of NAFLD was positively associated with CKD stages. Limitations of analysis are the relatively small size of studies utilizing liver histology and the suboptimal sensitivity of ultrasound and biochemistry for NAFLD detection in population-based studies. The and
Performance Evaluation on OpenGIS Consortium for Sensor Web Enablement Services

—The aim of this paper is to describe a performance evaluation of the interface model of Sensor Web Enablement, especially highlighting the Sensor Observation Service, Sensor Event Service and Sensor Instance Registry. These standards provide a transparent and interoperable way to access data measured by sensors. Studies found in the literature do not treat a performance evaluation on highlighted services in a detailed way. So, the performance evaluation in our study considers several factors that can inﬂuence the access time on these services. The results show an important inﬂuence of different ﬁlter types in the service response times. The result analysis demonstrated that the implementation of application that uses these services should be careful on use of these ﬁlters, as, due their deﬁnition, the performance of these applications can decrease.
CHAPTER 24 Musical potential

There is general agreement that music is a universal trait of humankind (Blacking 1995), that Homo sapiens as a species has the propensity for musical development, and that musical potential is as universal as linguistic ability (Wallin et al. 2000). An ongoing controversy persists however, concerning the extent of individual variability in musical potential and the extent to which observable differences in acquiring musical skills result from socialcontexts that facilitate learning, genetic· factors, or interactions between the two. This chapter outlines key elements of these debates and also considers how 'musical potential' has been assessed.
HOW TO DO GENDER WITH SHOPPING SPACE DESIGN?

This article has a triple assignment. First, I will briefly review the body of knowledge on gender performance and practice theory, which pertains to design of shopping space, to present dual nature of shopping and its space, in which it is the basic hypothesis of this article. Second, equally importantly, I will locate effective empirical research established on the S-O-R pattern with main findings in fields of consumer’s psychology and behavior related with environment design. Last, and of the most challenge, my specific intent is to clarify the network weaved by three dimensions, involving women’s gender identity as pleasure hunter, space design as acting stage and consumption as social practice. Space designers, with gender awareness highlighted here, would be more sensible and caring in future projects. Design, in this case, can be approaching its maximum value for the our society – humanity and harmony.
A large-strain, fast-response, and easy-to-manufacture electrothermal actuator based on laser-reduced graphene oxide

In this paper, we have developed a high-performance graphene electrothermal actuator (ETA). The fabrication method is easy, fast, environmentally friendly, and suitable for preparing both large-size and miniature graphene ETAs. When applied with the driving voltage of 65 V, the graphene ETA achieves a large bending angle of 270° with a fast response of 8 s and the recovery process costs 19 s. The large bending deformation is reversible and can be precisely controlled by the driving voltage. A simple robotic hand prepared by using a single graphene ETA can hold the object, which is more than ten times the weight of itself. By virtue of its large-strain, fast response, and easy-to-manufacture, we believe that the graphene ETA has tremendous potential in extensive applications involving biomimetic robotics, artificial muscles, switches, and microsensors in both macroscopic and microscopic fields.
Structure–Function Relations of the First and Fourth Extracellular Linkers of the Type IIa Na (cid:1) /P i Cotransporter: II. Substrate Interaction and Voltage Dependency of Two Functionally Important Sites

Functionally important sites in the predicted ﬁrst and fourth extracellular linkers of the type IIa Na (cid:1) /P i cotransporter (NaPi-IIa) were identiﬁed by cysteine scanning mutagenesis (Ehnes et al., 2004). Cysteine substitution or modiﬁcation with impermeant and permeant methanethiosulfonate (MTS) reagents at certain sites resulted in changes to the steady-state voltage dependency of the cotransport mode (1 mM P i , 100 mM Na (cid:1) at pH 7.4) of the mutants. At Gly-134 (ECL-1) and Met-533 (ECL-4), complementary behavior of the voltage dependency was documented with respect to the effect of cys-substitution and modiﬁcation. G134C had a weak voltage dependency that became even stronger than that of the wild type (WT) after MTS incubation. M533C showed a WT-like voltage dependency that became markedly weaker after MTS incubation. To elucidate the underlying mechanism, the steady-state and presteady-state kinetics of these mutants were studied in detail. The apparent afﬁnity constants for P i and Na (cid:1) did not show large changes after MTS exposure. However, the dependency on external protons was changed in a complementary manner for each mutant. This suggested that cys substitution at Gly-134 or modiﬁcation of Cys-533 had induced similar conformational changes to alter the proton modulation of transport kinetics. The changes in steady-state voltage dependency correlated with changes in the kinetics of presteady-state charge movements determined in the absence of P i , which suggested that voltage-dependent transitions in the transport cycle were altered. The steady-state and presteady-state behavior was simulated using an eight-state kinetic model in which the transition rate constants of the empty carrier and translocation of the fully loaded carrier were found to be critical determinants of the transport kinetics. The simulations predict that cys substitution at Gly-134 or cys modiﬁcation of Cys-533 alters the preferred orientation of the empty carrier from an inward to outward-facing conformation for hyperpolarizing voltages.
Product of Free Radical Injury and Antioxidant Status in Patients with Gestational Diabetes Mellitus (GDM)

Objective: This was to determine oxidant and antioxidant status of patients with GDM Methods : Total of 31 pregnant women with GDM and 51 patients with physiologic pregnancy were successfully recruited into the study. Gestational diabetes mellitus was diagnosed taking into consideration the guidelines of IADPSG and WHO in the course of oral glucose tolerance test (OGTT). Blood sample was also taken for the laboratory analysis of malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). Results : Higher plasma concentration of MDA though not statistically significant (p=0.305) was observed in patients with GDM (5.75±0.60 µmol/l) than patients with Non GDM (4.55±0.55 µmol/l). Lower plasma concentrations of GPx (2.98±1.10 Vs 3.12±0.78 U/ml, p=0.017) and SOD (69.33±21.94 vs86.47±24.26 U/ml, p=0.62) were observed in patients with GDM than patients without GDM Conclusion: The study shows release of product of free radical injury (MDA) in excess of antioxidants (GPx and SOD) in patients with gestational diabetes mellitus.
on Src Family Kinases and PI3K Macrophages through a Pathway Dependent A Induces Activation of p21Ras in Primary Pure Lipopolysaccharide or Synthetic Lipid

2 Recognition of bacterial LPS by macrophages plays a critical role in host defense against infection by Gram-negative bacteria. However, when not tightly regulated, the macrophage’s response to LPS can induce severe disease and septic shock. Although LPS triggers the activation of multiple signaling pathways in macrophages, it was unclear whether these include activation of the p21Ras GTPases. We report that p21Ras is rapidly and transiently activated in murine primary macrophages stimulated with an ultra-pure preparation of LPS or with synthetic lipid A. The molecular basis of this activation was investigated using a pharmacological approach. LPS-induced activation of p21Ras was inhibited in the presence of PP2, LY294002, or wortmannin, suggesting that it depends on the activity of one or more members of the Src kinase family and the subsequent activation of PI3K. In that pharmacological inhibitors of PI3K inhibited LPS-induced activation of p21Ras, but not activation of ERK, we concluded that LPS-induced activation of ERK occurs through a pathway that is not dependent on the activation of p21Ras. The Journal of Immunology, 2005, 175: 8236–8241.
Spinal administration of a d opioid receptor agonist attenuates hyperalgesia and allodynia in a rat model of neuropathic pain

Neuropathic (NP) pain is a debilitating chronic pain disorder considered by some to be inherently resistant to therapy with tra-ditional analgesics. Indeed, l opioid receptor (OR) agonists show reduced therapeutic beneﬁt and their long term use is hindered by the high incidence of adverse eﬀects. However, pharmacological and physiological evidence increasingly suggests a role for d OR agonists in modulating NP pain symptoms. In this study, we examined the antihyperalgesic and antiallodynic eﬀects of the spinally administered d OR agonist, D -[Ala 2 , Glu 4 ]deltorphin II (deltorphin II), as well as the changes in d OR expression, in rats following chronic constriction injury (CCI) of the sciatic nerve. Rats with CCI exhibited cold hyperalgesia and mechanical allodynia over a 14-day testing period. Intrathecal administration of deltorphin II reversed cold hyperalgesia on day 14 and dose-dependently attenuated mechanical allodynia. The eﬀects of deltorphin II were mediated via activation of the d OR as the eﬀect was antagonized by co-treatment with the d -selective antagonist, naltrindole. Western blotting experiments revealed no changes in d OR protein in the dorsal spinal cord following CCI. Taken together, these data demonstrate the antihyperalgesic and antiallodynic eﬀectiveness of a spinally administered d OR agonist following peripheral nerve injury and support further investigation of d ORs as potential therapeutic targets in the treatment of NP pain.
In pursuit of the longevity dividend

Imagine an intervention, such as a pill, that could significantly reduce your risk of cancer. Imagine an intervention that could reduce your risk of stroke, or dementia, or arthritis. Now, imagine an intervention that does all these things, and at the same time reduces your risk of everything else undesirable about growing older: including heart disease, diabetes, alzheimer and parkinson disease, hip fractures, osteoporosis, sensory impairments, and sexual dysfunction. Such a pill may sound like fantasy, but aging interventions already do this in animal models. And many scientists believe that such an intervention is a realistically achievable goal for people. People already place a high value on both quality and length of life, which is why children are immunized against infectious diseases. In the same spirit, we suggest that a concerted effort to slow aging begin immediately - because it will save and extend lives, improve health, and create wealth.