Miracle-dz/UniFAR-FTUs huggingface dataset:
High productivity of immunostimulatory membrane vesicles of Limosilactobacillus antri using glycine

Nanosized membrane vesicles (MVs) released by bacteria play important roles in both bacteria–bacteria and bacteria–host interactions. Some gram-positive lactic acid bacteria produce MVs exhibiting immunoregulatory activity in the host. We found that both bacterial cells and MVs of Limosilactobacillus antri JCM 15950, isolated from the human stomach mucosa, enhance immunoglobulin A production by murine Peyer’s patch cells. However, the thick cell walls of gram-positive bacteria resulted in low MV production, limiting experiments and applications using MVs. In this study, we evaluated the effects of glycine, which inhibits cell wall synthesis, on the immunostimulatory MV productivity of L. antri. Glycine inhibited bacterial growth while increasing MV production, with 20 g/L glycine increasing MV production approximately 12-fold. Glycine was most effective at increasing MV production when added in the early exponential phase, which indicated that cell division in the presence of glycine increased MV production. Finally, glycine increased MV productivity approximately 16-fold. Furthermore, glycine-induced MVs promoted interleukin-6 production by macrophage-like J774.1 cells, and the immunostimulatory activity was comparable to that of spontaneously produced MVs. Our results indicate that glycine is an effective agent for improving the production of MVs with immunostimulatory activity in gram-positive lactic acid bacteria, which can be applied as mucosal adjuvants and functional foods.
Perennial pastures on certified organic properties in South East Australia - a survey of farmer practices and awareness.

A survey of 166 organic producers located in Victoria, New South Wales and South Australia was conducted during 2005-2006 to determine the status of perennial pastures on their farms. A response rate of 71% was achieved. Survey respondents were experienced with an average of 16 years organic farming experience. Respondents had a high level of awareness of native grass species; and some had used management strategies to encourage them. Over half (55%) of respondents had established a perennial pasture since they had been farming organically, with lucerne and perennial ryegrass the most common species sown; lucerne and phalaris were the most common perennial species currently growing. These species, together with fertiliser (and legume inoculum) were typically broadcast into seed-beds, which had been cultivated to control weeds. More demonstration and evaluation of perennial species is required so that producers can make informed choices about species selection and management for their particular conditions.
Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities

Acetic acid, a colourless liquid organic acid with a characteristic acrid smell, is obtained naturally and has applications in both the food and pharmaceutical industries. It has been reported to have beneficial uses for lifestyle-related diseases, and its efficient disinfectant properties are well known. In this study, an alginate crosslinked with Ca2+ hydrogel film was treated with acetic acid to explore its biological properties for biomedicine. The results showed that the novel calcium alginate/acetic acid film was biocompatible in vitro using human keratinocyte cells and in vivo with Caenorhabditis elegans. It also had antiviral properties against enveloped and non-enveloped viruses and anticancer properties against melanoma and colon cancer cells. This novel film thus showed promise for the biomedical and pharmaceutical industries, with applications for fabricating broad-spectrum antiviral and anticancer materials.
Characterization of an Mtbp Hypomorphic Allele in a Diethylnitrosamine-Induced Liver Carcinogenesis Model

Simple Summary The MDM2 binding protein MTBP is implicated in various cellular functions and cancer-related processes, which vary depending on the cellular context and its localization within the cell. Moreover, the in vivo physiological function of MTBP remains unclear. To overcome embryonic lethality due to complete deletion of the Mtbp gene in mice, we created mice with an Mtbp hypomorphic allele (MtbpH) that expresses Mtbp protein at approximately 30% of the wild-type level. In a carcinogen-induced liver cancer model, MtbpH/− mice showed worse overall survival than wild-type mice. MEFs generated from the MtbpH/− mice displayed an increased nuclear localization of p-Erk1/2 protein and enhanced migratory potential. Thus, the newly generated MtbpH/− mice and MEFs can be used to study the in vivo physiological function of Mtbp and validate its diverse functions observed in human cells. Abstract MTBP is implicated in cell cycle progression, DNA replication, and cancer metastasis. However, the function of MTBP remains enigmatic and is dependent on cellular contexts and its cellular localization. To understand the in vivo physiological role of MTBP, it is important to generate Mtbp knockout mice. However, complete deletion of the Mtbp gene in mice results in early embryonic lethality, while its heterozygous deletion shows modest biological phenotypes, including enhanced cancer metastasis. To overcome this and better characterize the in vivo physiological function of MTBP, we, for the first time, generated mice that carry an Mtbp hypomorphic allele (MtbpH) in which Mtbp protein is expressed at approximately 30% of that in the wild-type allele. We treated wild-type, Mtbp+/−, and MtbpH/− mice with a liver carcinogen, diethylnitrosamine (DEN), and found that the MtbpH/− mice showed worse overall survival when compared to the wild-type mice. Consistent with previous reports using human liver cancer cells, mouse embryonic fibroblasts (MEFs) from the MtbpH/− mice showed an increase in the nuclear localization of p-Erk1/2 and migratory potential. Thus, MtbpH/− mice and cells from MtbpH/− mice are valuable to understand the in vivo physiological role of Mtbp and validate the diverse functions of MTBP that have been observed in human cells.
Renal Profile of Patients with Cardiorenal Syndrome: Nephrology and Cardiology Department Experience of the University Hospital IBN SINA of Rabat

Introduction: Cardio-renal syndrome (CRS) is a complex pathophysiological entity affecting the heart and kidneys in which acute or chronic dysfunction of one organ can induce acute or chronic dysfunction of the other organ. Five types of CRS have been described. Methods: The study explored the prevalence and types of Cardiorenal Syndrome (CRS) at CHU Ibn Sina in Rabat. Over a year, 120 CRS patients were assessed, excluding those with endstage chronic renal failure. We analyzed the epidemiological, clinical, therapeutic and evolutionary profile of these patients. Results: The average age of our patients is 67.8 ± 12 years, with extremes ranging from 39 years to 92 years. The sex ratio is 1.35. The different types of CRS types (1, 2, 4 and 5) were noted respectively in 28.4%, 20.8%, 5%, 45.8%, however, we did not note patients having CRS type 3. On the renal level, we noted acute renal failure (ARF) in 51.6% of patients,
A method for obtaining 3-D data in alpine skiing using pan-and-tilt cameras with zoom lenses

The direct linear transformation (DLT) was applied for three-dimensional reconstruction in Alpine skiing. Because of the large field of view it is necessary to follow the skier with the cameras to receive frames with a suciently large image of the skier. In our implementation, the DLT parameters are computed for each frame of each camera separately. Consequently it is possible to rotate the cameras and to zoom the lenses. The locations of the cameras need not be known. Control points were distributed on the slope. Their coordinates were determined by geode- tic surveying. The method requires at least six control points visible in each frame. Using more than six control points reduces the reconstruction error considerably. Under optimal conditions the mean errors are less than 5 cm in each coordinate. This accuracy is sucient for inverse dynamics if the data are smoothed and the
Influence of seasonality and gonad development on the nutritional quality and safety of the razor clam Sinonovacula constricta (Lamarck 1818)

Understanding the reproductive activity or seasonal variation in comprehensive evaluations of the nutritional quality and safety of the razor clam Sinonovacula constricta (Lamarck) is important for improving product quality and economic benefits. The contents of moisture (from 80.12±0.38 to 84.98±0.40%), protein (from 53.14±0.06 to 63.12±0.69% DW), lipid (from 4.55±0.19 to 5.69±0.35% DW), glycogen (from 5.40±1.11 to 16.13±1.55% DW), and ash (from 12.03±0.33 to 19.86±0.56% DW) varied significantly at different gonadal development cycles or seasons. Glycogen, lipid, and protein variations were closely associated with gonadal development, with the lowest levels of lipid and protein in the spawning period. Moreover, S. constricta consistently had a well-balanced essential amino acid profile throughout the year, indicating that it is a source of high-quality protein. The predominant free amino acids in S. constricta were taurine, glycine, lysine, and alanine, which are important contributors to the flavor. S. constricta was defined as lean fat throughout the gonad cycle with polyunsaturated fatty acid accounting for the majority in the active and maturing stages. Microbiological quality and total volatile basic nitrogen were associated with the alteration of seasons. The optimal harvesting time of S. constricta was the inactive stage of most gonads in spring (from March to April in Taizhou, Zhejiang Province, China).
Lithostratigraphy of the Adnet Group (Lower to Middle Jurassic, Salzburg, Austria)

The Adnet Group as exposed at the Adnet quarries and the Osterhorn Mts. southeast of Salzburg is described and formally defined. Two formations are distinguished. The Schnöll Formation (Hettangian) at the base of the Adnet Group formed as a wedge at the slope of the drowned Rhaetian Adnet reef. The Adnet Formation is much more widespread in the type area and persisted with only slight facies variations from the Sinemurian until the early Middle Jurassic. The Schnöll Formation is subdivided into two members. The Langmoos Member is characterized by siliceous sponges, while crinoidal debris is the major component of the overlying Guggen Member. A similar facies succession is also found in the contemporaneous Kendlbach Formation and points to a regional change of environmental conditions, like oxygenation, current activities and nutrient levels in the wake of the platform drowning event of the Triassic-Jurassic boundary. Six members make up the Adnet Formation. Three members formed simultaneously during the Sinemurian in different positions on the drowned Rhaetian reef slope and in the adjacent basin (Lienbacher, Motzen and Schmiedwirt Member). Three more members (Kehlbach, Scheck, Saubach) formed as a sequence of increasingly pelagic sedimentation during the middle and late Liassic. The Saubach Member continued into the early Middle Jurassic. Intercalated breccias, especially the breccia layers forming the Scheck Member, are evidence for tectonic activities culminating during the late Pliensbachian and early Toarcian. The trend to more pelagic conditions continued during the Middle Jurassic with the plankton dominated limestones of the Klaus Formation and the radiolarites of the Ruhpolding Group.
The Treatment Landscape of Elderly Patients with Hormone Receptor-Positive Her2 Negative Advanced Breast Cancer: Current Perspectives and Future Directions

Breast cancer (BC) in elderly women is an increasing health issue due to demographic changes. BC tends to present later and may receive less than standard treatment options. More often, BC in elderly patients is endocrine-positive (HR+). The treatment of elderly patients with metastatic BC (mBC) represents a therapeutic challenge. In recent years, the treatment landscape of patients that are HR+/Her2-negative has changed due to the introduction in clinical practice of new targeted drugs, which have improved patient outcomes. Elderly patients are a small percentage of all patients enrolled in clinical trials and, to date, there are no standardized guidelines that define the best treatment option for this patient population. This can lead to undertreatment or overtreatment, impacting patient morbidity and mortality. Geriatric Assessment tools to tailor the treatment in elderly patients are underused because they are long and difficult to apply in a busy routine clinical practice. For all these reasons, there is an urgent need to produce data about the best treatment for elderly patients with HR+ mBC. Herein, we report data from randomized clinical trials and real-world evidence on the therapeutic options for HR+ Her2-negative mBC elderly patients and explore future treatment directions.
Relationship Between Eating Behaviors and Physical Activity with Blood Glucose Levels in Type 2 Diabetes Mellitus Patients in Gayaman Public Health Center Mojokerto

Introduction: Diabetes mellitus can be caused by an unhealthy lifestyle including poor eating behavior and mild physical activity which can affect glucose metabolism so that blood glucose levels are high. This study aims to determine the relationship between eating behavior and physical activity with blood glucose levels in type 2 diabetes mellitus patients. Method: The study design used correlation analysis with a cross-sectional approach. The population in this study was all type 2 diabetes mellitus patients at the Gayaman Public Health Center Mojokerto. The sampling technique of this study was consecutive sampling. The research instrument used an eating behavior questionnaire adopted from the Aethelstone and physical activity level (PAL) questionnaire and was analyzed using the Spearman Rho test. Results: The results showed that 44.6% of respondents had poor eating behaviors, 48.2% had mild physical activity, and 80.4% had high blood glucose levels. The results of the Spearman Rho test showed that there was a significant relationship between eating behavior with blood glucose levels (p value=0,001;α<0,05) and physical activity with blood glucose levels (p value=0,002;α<0,05) in patients with type 2 diabetes mellitus at the Gayaman Public Health Center Mojokerto. Conclusion: Poor eating behaviors tend to cause high blood glucose levels because of excessive intake of food and drinks that contain carbohydrates which are metabolized to be glucose, if the patient is not doing physical activity then the glucose levels become high. The importance of controlling blood glucose levels in Type 2 Diabetes Mellitus patients by improving eating behavior includes an eating schedule, reducing food and drinks that contain a lot of glucose, and increasing physical activity
Gait Phase Recognition Based on Improved Multi-Objective Sine-Cosine Algorithm

Gait phase recognition has been shown to facilitate the analysis of gait feature, evaluation of ankle rehabilitation, and assessment of physical ability. However, the efficiency of gait phase recognition is generally limited by the shortcoming of single sensor and data acquisition method. The application of multi-sensor fusion improves the recognition accuracy, whereas it increases data redundancy and reduces system real-time performance. This paper proposes an improved multi-objective sine-cosine algorithm for gait phase recognition which aims to identify the most effective feature combinations to achieve optimal recognition accuracy. We explore the effect of different sensor positions on gait phase recognition, and introduce integrated classifiers and multi-sensor to improve the performance. Experimental results demonstrate that the proposed method effectively increases the convergence speed, avoids local optimal solutions, reduces data dimensionality, and recognizes gait phases. The proposed method has the potential to provide effective support for personalized control in gait rehabilitation.
Effects of probiotics (bacillus subtilis and bacillus licheniformis) on the performance and antimicrobial activity of broiler chicken

This study was conducted to investigate the efficacy of probiotics on the performance and antimicrobial activity of broiler. A total of 300 oneday old of Cobb-500 broiler chicks were divided into five experimental groups (T1-T5) in completely randomized design with six replicates as ten chicks in each replication. One of the five experimental groups fed diet without probiotic was as control (T1) and the remaining four groups fed diet with 4 levels of commercial probiotics were 20g BS/metric ton feed (T2), 50g BS/metric ton feed (T3), 20g BL/metric ton feed (T4) and 50g BL/metric ton feed (T5). Among the groups, T5 showed significantly (P˂0.05) higher body weight and lower feed intake than the others. Feed conversion ratio (FCR) was better in all probiotic treated groups compared to the control and the best FCR was found in T5 group. The weight of breast, wing and giblet was significantly (P
Determination of the shear viscosity of the sclera

At present information about viscous properties of the eye's sclera is scarce, the main reason being that direct measurements cause technical problems. Nevertheless, coefficients of the sclera viscosity are of paramount interest for biomechanics and ophthalmologists. This paper investigates a method for determining the shear viscosity of the sclera based on a comparison of results from mathematical modeling with estimated experimental data from measurements of the IntraOcular Pressure (IOP) over a long time after an intravitreal injection (injection into the vitreous body of the eyeball) into enucleated eyes. In order to model the IOP behavior we consider a viscoelastic spherical layer subjected to a centrally symmetric load as follows. The excess pressure is considered, hence, the external pressure is absent and a displacement of the inner boundary is specified in order to take the intravitreal injection volume into account. We assume that during the experiment the volume of the eyeball remains constant and that the material of the sclera obeys linear transversely isotropic elasticity. The function for the IOP, which is based on fitted experimental data presented in the literature, decreases up to a limit value. As a result, we obtain a value for the shear viscosity of the sclera corresponding to the best adjustment between experimental data and theoretical prediction.
FIELD EXPERIMENTS O F D ISCRIMINATION IN T HE MARKET PLACE

Controlled experiments, using matched pairs of bogus transactors, to test for discrimination in the marketplace have been conducted for over 30 years, and have extended across 10 countries. Significant, persistent and pervasive levels of discrimination have been found against nonwhites and women in labour, housing and product markets. Rates of employment discrimination against non-whites, in excess of 25% have been measured in Australia, Europe and North America. A small number of experiments have also investigated employment discrimination against the disabled in Britain and the Netherlands, and against older applicants in the United
The Role of Status Consumption and Materialism in Influencing Attitude towards Counterfeit Fashion Product

The act of counterfeiting spans across various industries and the impact can be seen beyond economic factors. In consumer behavior, the impact of status consumption and materialism has influenced purchase decisions in which consumption is not only based on how it makes an individual feel but also on those around them. Ultimately, the extent to which consumers may go in terms of getting status recognition influenced them to look for “cheaper” alternatives to genuine manufactured products. Hence the demand for counterfeit fashion products kept rising over the years. This research shows the impact of materialism and status consumption in influencing consumer attitudes toward counterfeit fashion products. Data were analyzed using a partial least square (PLS) approach to structural equation modeling (SEM). Materialism is significant in influencing attitude whereas there was no significant relationship found between status consumption and attitude.
Recent genome resequencing paraded COBRA-Like gene family roles in abiotic stress and wood formation in Poplar

A cell wall determines the mechanical properties of a cell, serves as a barrier against plant stresses, and allows cell division and growth processes. The COBRA-Like (COBL) gene family encodes a putative glycosylphosphatidylinositol (GPI)-anchored protein that controls cellulose deposition and cell progression in plants by contributing to the microfibril orientation of a cell wall. Despite being studied in different plant species, there is a dearth of the comprehensive global analysis of COBL genes in poplar. Poplar is employed as a model woody plant to study abiotic stresses and biomass production in tree research. Improved genome resequencing has enabled the comprehensive exploration of the evolution and functional capacities of PtrCOBLs (Poplar COBRA-Like genes) in poplar. Phylogeny analysis has discerned and classified PtrCOBLs into two groups resembling the Arabidopsis COBL family, and group I genes possess longer proteins but have fewer exons than group II. Analysis of gene structure and motifs revealed PtrCOBLs maintained a rather stable motif and exon–intron pattern across members of the same group. Synteny and collinearity analyses exhibited that the evolution of the COBL gene family was heavily influenced by gene duplication events. PtrCOBL genes have undergone both segmental duplication and tandem duplication, followed by purifying selection. Promotor analysis flaunted various phytohormone-, growth- and stress-related cis-elements (e.g., MYB, ABA, MeJA, SA, AuxR, and ATBP1). Likewise, 29 Ptr-miRNAs of 20 families were found targeting 11 PtrCOBL genes. PtrCOBLs were found localized at the plasma membrane and extracellular matrix, while gene ontology analysis showed their involvement in plant development, plant growth, stress response, cellulose biosynthesis, and cell wall biogenesis. RNA-seq datasets depicted the bulk of PtrCOBL genes expression being found in plant stem tissues and leaves, rendering mechanical strength and rejoinders to environmental cues. PtrCOBL2, 3, 10, and 11 manifested the highest expression in vasculature and abiotic stress, and resemblant expression trends were upheld by qRT-PCR. Co-expression network analysis identified PtrCOBL2 and PtrCOBL3 as hub genes across all abiotic stresses and wood developing tissues. The current study reports regulating roles of PtrCOBLs in xylem differentiating tissues, tension wood formation, and abiotic stress latency that lay the groundwork for future functional studies of the PtrCOBL genes in poplar breeding.
Swahili Loanwords and their Semantic Nativisations into African Languages

Traditionally, contributions of loanwords to the morphology of most African languages are recognizable in terms of phoneme inventories, consonant clusters, and syllable structures. The present paper, however, articulates the semantic and pragmatic issues emanating from the Swahili loanwords into the morphology of most African languages in Tanzania. The focus is mainly on the influence of Swahili into the different semantic fields in ten Bantu languages in the country. Data of 500 lexical words is drawn from a sample of Bantu languages in the country. Formal interviews had also been conducted to decipher semantic and pragmatic inferences springing out of the usages of those loanwords in the respective speech communities. It is concluded that most semantic fields of loanwords include education, utensils and domestic tools, as well as health and medical care services. In addition, pragmatically, some loanwords divide communities according to their usages, e.g. in Ruhaya and Runyambo, the entries for church include ekerezia [< ecclesia, ecclesiae Latin] which is dominantly used for Roman Catholic churches while ekanisa [