Miracle-dz/UniFAR-FTUs huggingface dataset:
SARS-CoV-2 in Animal Companions: A Serosurvey in Three Regions of Southern Italy

Several animal species have been found to be susceptible to SARS-CoV-2 infection. The occurrence of infection in dogs and cats living in close contact with owners deserves particular attention from public health authorities in a One Health approach. In this study, we conducted serological screening to identify SARS-CoV-2 exposure in the sera from dogs and cats in three regions of southern Italy sampled during the years 2021 and 2022. We collected 100 serum samples in 2021 (89 from dogs and 11 from cats) and 640 in 2022 (577 from dogs and 63 from cats). Overall, the ELISA positivity rate was found to be 2.7% (20/740), with higher seroprevalence in dogs. Serum neutralization tests confirmed positivity only in two samples collected from dogs, and the assays, performed with serologically distinct SARS-CoV-2 variants, showed variant-specific positivity. This paper shows that monitoring SARS-CoV-2 exposure in animals might be affected by the viral antigenic evolution, which requires continuous updates to the serological tests used. Serological surveys are useful in understanding the true extent of exposure occurring in specific animal populations, not suffering the same limitations as molecular tests, and could help in identifying the infecting virus if tests able to characterize the immune response are used. The use of variant-specific validated serological methods should always be considered in serosurvey studies in order to determine the real impact of emerging variants on animal populations and its implications for veterinary and human health, as well as to identify potential reservoirs of the virus and its evolutionary changes.
Occurrence of mastitis pathogens at dry off period and after calving in dairy cows

The aim of the work was to study the occurrence of mastitis pathogens before and after calving in the same dairy cows. The Holstein cows suspicious on subclinical mastitis (positive California Mastitis Test) were sampled at quarter level under practical farm with high level of milk yield (11,278 kg). The cows were treated with antibiotics (Cefquinomum) before drying. In total 84 samples before drying and 107 samples after calving from the same dairy cows were collected. The samples were cultured on blood agar (MkB Test as, Rosina, SR). MALDI-TOF MS (Bruker Daltonics, Germany) was used to identify mastitis pathogens. Bacteriologically positive (BP) samples from dairy cows before drying were found in 35% of the milk samples. The most frequent pathogens in BP milk samples were coagulase-negative staphylococci (CNS) (77.38%). The most common CNS was Staphylococcus (S.) xylosus (32.14%). S. aureus was detected in 5.95% of BP samples. After calving, we found BP samples in 14.02% of dairy cows. The most common pathogens in milk samples were CNS (10.39%). S. aureus was detected in 0.94% of BP samples. Antibiotic treatment during the drying period clearly reduced the occurrence of CNS and S. aureus in dairy cows at the beginning of lactation.
What do planetary nebulae and H II regions reveal about the chemical evolution of nearby dwarf galaxies?

Abstract The Local Group contains a great number of dwarf irregulars and spheroidals, for which the spectroscopy of individual stars can be obtained. Thus, the chemical evolution of these galaxies can be traced, with the only need of finding populations spanning a large age range and such that we can accurately derive the composition. Planetary nebulae (PNe) are old- and intermediate-age star remnants and their chemical abundances can be obtained up to 3-4 Mpc. H ii regions, which are brighter and much easily detected, represent galaxies young content. PNe and H ii regions share similar spectroscopic features and are analysed in the same way. Both are among the best tracers of the chemical evolution allowing to draw the chemical time line of nearby galaxies. The focus in this review are the PN and H ii region populations as constraints to the chemical evolution models and the mass-metallicity relation of the local universe.
Gut Microbiota’s Oxalate-Degrading Activity and Its Implications on Cardiovascular Health in Patients with Kidney Failure: A Pilot Prospective Study

Background and Objectives: The present study aims to investigate the association between gut microbiota’s oxalate-degrading activity (ODA) and the risk of developing cardiovascular disease (CVD) over a three-year follow-up period in a cohort of patients undergoing kidney replacement therapy (KRT). Additionally, various factors were examined to gain insight into the potential mechanisms underlying the ODA–CVD link. Materials and Methods: A cohort of 32 KRT patients and 18 healthy volunteers was enrolled in this prospective observational pilot study. Total fecal ODA, routine clinical data, plasma oxalic acid (POx), serum indoxyl sulfate, lipid profile, oxidative stress, and proinflammatory markers were measured, and the patients were followed up for three years to assess CVD events. Results: The results revealed that patients with kidney failure exhibited significantly lower total fecal ODA levels compared to the healthy control group (p = 0.017), with a higher proportion showing negative ODA status (≤−1% per 0.01 g) (p = 0.01). Negative total fecal ODA status was associated with a significantly higher risk of CVD events during the three-year follow-up period (HR = 4.1, 95% CI 1.4–16.3, p = 0.003), even after adjusting for potential confounders. Negative total fecal ODA status was significantly associated with elevated POx and indoxyl sulfate levels and linked to dyslipidemia, increased oxidative stress, and inflammation, which are critical contributors to CVD. Conclusions: The findings contribute novel insights into the relationship between gut microbiota’s ODA and cardiovascular health in patients undergoing KRT, emphasizing the need for further research to elucidate underlying mechanisms and explore potential therapeutic implications of targeting gut microbiota’s ODA in this vulnerable population.
The Integral Role of Magnesium in Muscle Integrity and Aging: A Comprehensive Review

Aging is characterized by significant physiological changes, with the degree of decline varying significantly among individuals. The preservation of intrinsic capacity over the course of an individual’s lifespan is fundamental for healthy aging. Locomotion, which entails the capacity for independent movement, is intricately connected with various dimensions of human life, including cognition, vitality, sensory perception, and psychological well-being. Notably, skeletal muscle functions as a pivotal nexus within this intricate framework. Any perturbation in its functionality can manifest as compromised physical performance and an elevated susceptibility to frailty. Magnesium is an essential mineral that plays a central role in approximately 800 biochemical reactions within the human body. Its distinctive physical and chemical attributes render it an indispensable stabilizing factor in the orchestration of diverse cellular reactions and organelle functions, thereby rendering it irreplaceable in processes directly impacting muscle health. This narrative review offers a comprehensive exploration of the pivotal role played by magnesium in maintaining skeletal muscle integrity, emphasizing the critical importance of maintaining optimal magnesium levels for promoting healthy aging.
QUANTIFICATION OF MYOCARDIAL MECHANICS IN LEFT VENTRICLES UNDER INOTROPIC STIMULATION AND IN HEALTHY RIGHT VENTRICLES USING 3D DENSE CMR

OF DISSERTATION QUANTIFICATION OF MYOCARDIAL MECHANICS IN LEFT VENTRICLES UNDER INOTROPIC STIMULATION AND IN HEALTHY RIGHT VENTRICLES USING 3D DENSE CMR Statistical data from clinical studies indicate that the death rate caused by heart disease has decreased due to an increased use of evidence-based medical therapies. This includes the use of magnetic resonance imaging (MRI), which is one of the most common noninvasive approaches in evidence-based health care research. In the current work, I present 3D Lagrangian strains and torsion in the left ventricle of healthy and isoproterenolstimulated rats, which were investigated using Displacement ENcoding with Stimulated Echoes (DENSE) cardiac magnetic resonance (CMR) imaging. With the implementat ion of the 12-segment model, a detailed profile of regional cardiac mechanics was reconstructed for each subject. Statistical analysis revealed that isoproterenol induced a significant change in the strains and torsion in certain regions at the mid-ventricle level. In addition, I investigated right ventricular cardiac mechanics with the methodologies developed for the left ventricle. This included a comparison of different regions within the basal and mid-ventricular regions. Despite no regional variation found in the peak circumferential strain, the peak longitudinal strain exhibited regional variation at the anterior side of the RV due to the differences in biventricular torsion, mechanism of RV free wall contraction, and fiber architecture at RV insertions. Future applications of the experimental work presented here include the construction and validation of biventricular finite element models. Specifically, the strains predicted by the models will be statistica lly compared with experimental strains. In addition, the results of the present study provide an essential reference of RV baseline evaluated with DENSE MRI, a highly objective technique.
University of Birmingham The Acute Effects of Interval-Type Exercise on Glycemic Control in Type 2 Diabetes Subjects

Interval-type exercise is effective for improving glycemic control, but the optimal approach is unknown. The purpose of this study was to determine the importance of the interval length on changes in postprandial glycemic control following a single exercise bout. Twelve subjects with type 2 diabetes completed a cross-over study with three 1-hour interventions performed in a non-randomized but counter-balanced order: 1) Interval walking consisting of repeated cycles of 3 min slow (aiming for 54% of Peak oxygen consumption rate [VO 2 peak]) and 3 min fast (aiming for 89% of VO 2 peak) walking (IW3); 2) Interval walking consisting of repeated cycles of 1 min slow and 1 min fast walking (IW1) and 3) No walking (CON). The exercise interventions were matched with regards to walking speed, and VO 2 and heart rate was assessed throughout all interventions. A 4-hour liquid mixed meal tolerance test commenced 30 min after each intervention, with blood samples taken regularly. IW3 and IW1 resulted in comparable mean VO 2 and heart rates. Overall mean postprandial blood glucose levels were lower after IW3 compared to CON (10.3 ± 3.0 vs. 11.1 ± 3.3 mmol/ L; P < 0.05), with no significant differences between IW1 (10.5 ± 2.8 mmol/L) and CON or IW3 and IW1 (P > 0.05 for both). Conversely blood glucose levels at specific time points during the MMTT differed significantly following both IW3 and IW1 as
Transspinal Direct Current Electrical Stimulation Selectively Affects the Excitability of the Corticospinal System, Depending on the Intensity but Not Motor Skills

Transspinal direct current stimulation (tsDCS) is a non-invasive technique used to modulate spinal cord activity. However, the effects and mechanisms of this stimulation are currently not comprehensively known. This study aimed to estimate the effect of different intensities of tsDCS applied at the level of cervical enlargement of the spinal cord (C7-Th1 segments) on the excitability of the corticospinal system (CSS) and the correction of motor skills in healthy subjects. The effect of tsDCS was estimated by the motor-evoked potentials (MEP) elicited by transcranial magnetic stimulation (TMS) in the primary motor cortex (M1). The study involved 54 healthy adults aged 22 ± 4 years. The application of 11 min anodal tsDCS at the level of the cervical spine C7-Th1 with a current intensity of 2.5 mA did not change the MEP amplitude of the upper limb muscles, in contrast to the data that we previously obtained with a current intensity of 1.5 mA. We also found no difference in the effect of 2.5 mA stimulation on motor skill correction in healthy subjects in the nine-hole peg test (9-HPT) and the serial reaction time task (SRT) as with 1.5 mA stimulation. Our data show that an increase in the intensity of stimulation does not lead to an increase in the effects but rather reduces the effects of stimulation. These results provide information about the optimally appropriate stimulation current intensities to induce CSS excitability and the ability of tsDCS to influence motor skills in healthy adults.
The relationship between entrepreneurial leadership and employees' innovative behavior in the hospitality and tourism industry: The role of organizational agility

This study investigates how entrepreneurial leadership affects employee employees' innovative behavior in the hospitality and tourism industry through organizational agility as a mediator. PLS-SEM was used to analyze four hundred valid responses gathered from full-time employees working in five-star hotels and category (A) travel agencies operating in Egypt. Data analysis was performed using the WarpPLS 7.0 program. The findings indicated that entrepreneurial leadership is positively correlated with organizational agility and employees' innovative behavior. Findings also indicated organizational agility positively correlated with employees' innovative behavior. Furthermore, organizational agility acts as a mediator in the relationship between entrepreneurial leadership and employees' innovative behavior. The study advances the theoretical understanding of entrepreneurial leadership, organizational agility, and employees' innovative behavior in the context of Egypt's tourism and hospitality industry. Practically, the study provides tourism and hospitality enterprises with recommendations for maximizing the benefits of entrepreneurial leadership in terms of organizational agility and employees' innovative behavior.
Semi-automated motor hotspot search (SAMHS): a framework toward an optimised approach for motor hotspot identification

Background Motor hotspot identification represents the first step in the determination of the motor threshold and is the basis for the specification of stimulation intensity used for various Transcranial Magnetic Stimulation (TMS) applications. The level of experimenters’ experience and the methodology of motor hotspot identification differ between laboratories. The need for an optimized and time-efficient technique for motor hotspot identification is therefore substantial. Objective With the current work, we present a framework for an optimized and time-efficient semi-automated motor hotspot search (SAMHS) technique utilizing a neuronavigated robot-assisted TMS system (TMS-cobot). Furthermore, we aim to test its practicality and accuracy by a comparison with a manual motor hotspot identification method. Method A total of 32 participants took part in this dual-center study. At both study centers, participants underwent manual hotspot search (MHS) with an experienced TMS researcher, and the novel SAMHS procedure with a TMS-cobot (hereafter, called cobot hotspot search, CHS) in a randomized order. Resting motor threshold (RMT), and stimulus intensity to produce 1 mV (SI1mV) peak-to-peak of motor-evoked potential (MEP), as well as MEPs with 120% RMT and SI1mV were recorded as outcome measures for comparison. Results Compared to the MHS method, the CHS produced lower RMT, lower SI1mV and a trend-wise higher peak-to-peak MEP amplitude in stimulations with SI1mV. The duration of the CHS procedure was longer than that of the MHS (15.60 vs. 2.43 min on average). However, accuracy of the hotspot was higher for the CHS compared to the MHS. Conclusions The SAMHS procedure introduces an optimized motor hotspot determination system that is easy to use, and strikes a fairly good balance between accuracy and speed. This new procedure can thus be deplored by experienced as well as beginner-level TMS researchers.
Intergranular diffusion rates from the analysis of garnet surfaces: Implications for metamorphic equilibration

Novel approaches to garnet analysis have been used to assess rates of intergranular diffusion between different matrix phases and garnet porphyroblasts in a regionally metamorphosed staurolite‐mica‐schist from the Barrovian‐type area in Scotland. X‐ray maps and chemical traverses of planar porphyroblast surfaces reveal chemical heterogeneity of the garnet grain boundary linked to the nature of the adjacent matrix phase. The garnet preserves evidence of low temperature retrograde exchange with matrix minerals and diffusion profiles documenting cation movement along the garnet boundaries. Garnet–quartz and garnet–plagioclase boundaries preserve evidence of sluggish Mg, Mn and Fe diffusion at comparable rates to volume diffusion in garnet, whereas diffusion along garnet–biotite interfaces is much more effective. Evidence of particularly slow Al transport, probably coupled to Fe3+ exchange, is locally preserved on garnet surfaces adjacent to Fe‐oxide phases. The Ca distribution on the garnet surface shows the most complex behaviour, with long‐wavelength heterogeneities apparently unrelated to the matrix grain boundaries. This implies that the Ca content of garnet is controlled by local availability and is thought likely to reflect disequilibrium established during garnet growth. Geochemical anomalies on the garnet surfaces are also linked to the location of triple junctions between the porphyroblasts and the matrix phases, and imply enhanced transport along these channels. The slow rates of intergranular diffusion and the characteristics of different boundary types may explain many features associated with the prograde growth of garnet porphyroblasts. Thus, minerals such as quartz, Fe‐oxides and plagioclase whose boundaries with garnet are characterized by slow intergranular diffusion rates appear to be preferentially trapped as inclusions within porphyroblasts. As such grain boundary diffusion rates may be a significant kinetic impediment to metamorphic equilibrium and garnet may struggle to maintain chemical and textural equilibrium during growth in pelites.
Citizens’ Intention to Use the Palestinian e-Gov-ernment Services Portal – An Extension of UMEGA

: With the wide spread of Information and Communication Technologies (ICTs), governments are increasingly employing them to better satisfy the demands of their citizens, and to advance their vision of sustainable development. The purpose of this study is to investigate the determinants of Palestinian citizens’ attitudes and behavioural intentions to use the recently launched e-government services portal. Using a revised version of the Unified Model of Electronic Government Adoption (UMEGA) that incorporates a multidimensional construct of perceived risk construct, this study addresses a gap in the e-government literature where most previous studies have used perceived risk as a unidimensional construct. The study is a quantitative study that utilizes a combination of purposive and quota sampling to draw the sample, 415 valid responses were collected from the study population, and then analysed using PLS-SEM. The findings revealed that performance expectancy, effort expectancy, social influence and opportunity cost risk significantly influenced citizens' attitudes, which in turn, positively influenced their behavioural intentions
Design of Experiments to Compare the Mechanical Properties of Polylactic Acid Using Material Extrusion Three-Dimensional-Printing Thermal Parameters Based on a Cyber–Physical Production System

The material extrusion 3D printing process known as fused deposition modeling (FDM) has recently gained relevance in the additive manufacturing industry for large-scale part production. However, improving the real-time monitoring of the process in terms of its mechanical properties remains important to extend the lifespan of numerous critical applications. To enhance the monitoring of mechanical properties during printing, it is necessary to understand the relationship between temperature profiles and ultimate tensile strength (UTS). This study uses a cyber–physical production system (CPPS) to analyze the impact of four key thermal parameters on the tensile properties of polylactic acid (PLA). Layer thickness, printing speed, and extrusion temperature are the most influential factors, while bed temperature has less impact. The Taguchi L-9 array and the full factorial design of experiments were implemented along with the deposited line’s local fused temperature profile analysis. Furthermore, correlations between temperature profiles with the bonding strength during layer adhesion and part solidification can be stated. The results showed that layer thickness is the most important factor, followed by printing speed and extrusion temperature, with very close influence between each other. The lowest impact is attributed to bed temperature. In the experiments, the UTS values varied from 46.38 MPa to 56.19 MPa. This represents an increase in the UTS of around 17% from the same material and printing design conditions but different temperature profiles. Additionally, it was possible to observe that the influence of the parameter variations was not linear in terms of the UTS value or temperature profiles. For example, the increase in the UTS at the 0.6 mm layer thickness was around four times greater than the increase at 0.4 mm. Finally, even when it was found that an increase in the layer temperature led to an increase in the value of the UTS, for some of the parameters, it could be observed that it was not the main factor that caused the UTS to increase. From the monitoring conditions analyzed, it was concluded that the material requires an optimal thermal transition between deposition, adhesion, and layer solidification in order to result in part components with good mechanical properties. A tracking or monitoring system, such as the one designed, can serve as a potential tool for reducing the anisotropy in part production in 3D printing systems.
Stress-Strain Behavior of Granular Soils Subjected to Internal Erosion

AbstractInternal erosion is a major cause of dam or dike failures and incidents. In this study, laboratory tests were conducted to investigate the deformation of two gap-graded soils during internal erosion and the stress-strain behavior of the soils that experienced loss of fine particles due to internal erosion. An erosion-controlled experimental method was adopted to achieve a designated loss of fine particles during internal erosion by adding a predefined amount of salt into the soil sample during sample preparation and dissolving the salt in water under a controlled stress condition. Both the radial and axial deformations during erosion were measured using a photographic method. Subsequently, drained triaxial compression tests were performed to study the stress-strain behavior of the soils that had lost different amounts of fine particles. The peak friction angle and critical friction angle of the soil decreased with the loss of fine particles. After a significant loss of fine particles, the stress-s.
Sport-Sciences University students’ perceptions related to the use of debates and e-portfolios as student-centered learning strategies

This study analyzed Sports Science Degree university students’ perceptions about the use of debates and e-portfolio as student-centered learning strategies. The study involved 426 students from the third and last year of the program (23% females; mean age = 20.6 ± 1.1). A questionnaire was used to collect data, and thematic analysis revealed four positive (active learning, information exchange, innovation, and skills development) and three negative (ignorance of methodology, lack of interest/distraction, and extra work) themes related to these strategies. Despite some negative perceptions, students highlighted the positive aspects of these methods, indicating that incorporating them can improve learning outcomes.