Miracle-dz/UniFAR-FTUs huggingface dataset:
K+-Dependent Na+/Ca2+ Exchanger Isoform 2, Nckx2, Takes Part in the Neuroprotection Elicited by Ischemic Preconditioning in Brain Ischemia

Sodium/Calcium exchangers are neuronal plasma membrane antiporters which, by coupling Ca2+ and Na+ fluxes across neuronal membranes, play a relevant role in brain ischemia. The most brain-expressed isoform among the members of the K+-dependent Na+/Ca2+ exchanger family, NCKX2, is involved in the progression of the ischemic lesion, since both its knocking-down and its knocking-out worsens ischemic damage. The aim of this study was to elucidate whether NCKX2 functions as an effector in the neuroprotection evoked by ischemic preconditioning. For this purpose, we investigated: (1) brain NCKX2 expression after preconditioning and preconditioning + ischemia; (2) the contribution of AKT and calpain to modulating NCKX2 expression during preconditioning; and (3) the effect of NCKX2 knocking-out on the neuroprotection mediated by ischemic preconditioning. Our results showed that NCKX2 expression increased in those brain regions protected by ischemic preconditioning. These changes were p-AKT-mediated since its inhibition prevented NCKX2 up-regulation. More interestingly, NCKX2 knocking-out significantly prevented the protection exerted by ischemic preconditioning. Overall, our results suggest that NCKX2 plays a fundamental role in the neuroprotective effect mediated by ischemic preconditioning and support the idea that the enhancement of its expression and activity might represent a reasonable strategy to reduce infarct extension after stroke.
Chemical Composition of Cactus Pear Seed Oil: phenolics identification and antioxidant activity

Objectives The chemical composition of cactus pear seed oil (Opuntia ficus-indica [L.] Mill.) was analyzed in terms of its fatty acid composition, tocopherol content, phenolic identification, and the oil’s phenolic-rich fraction antioxidant power was determined. Methods Fatty acid profiling was performed by gas chromatography coupled to an FI detector. Tocopherols and phenolic compounds were analyzed by LC-FLD/UV, and the oil’s phenolic-rich fraction antioxidant power was determined by phosphomolybdenum, DPPH assay and β-carotene bleaching test. Results Fatty acid composition was marked by a high unsaturation level (83.22 ± 0.34%). The predominant fatty acid was linoleic acid (66.79 ± 0.78%), followed by oleic acid (15.16 ± 0.42%) and palmitic acid (12.70 ± 0.03%). The main tocopherol was γ-tocopherol (172.59 ± 7.59 mg/kg. In addition, Tyrosol, vanillic acid, vanillin, ferulic acid, pinoresinol, and cinnamic acid were identified as phenolic compounds in the analyzed seed oil. Moreover, the oil’s phenolics-rich fraction showed a significant total antioxidant activity, scavenged DPPH up to 97.85%, and effectively protected β-carotene against bleaching (97.56%). Conclusion The results support the potential use of cactus pear seed oil as a functional food.
Effect of Chirality on the Stability of Carbon Nanotubes

The effect of chirality on the structural stability of single-wall carbon nanotubes have been investigated by performing molecular-dynamics computer simulations. Calculations have been realized by using an empirical many-body potential energy function for carbon. It has been found that carbon nanotube in chiral structure is more stable under heat treatment relative to zigzag and armchair models. The diameter of the tubes is slightly enlarged under heat treatment.
Smart Charging Strategies for Electric Vehicles utilising: Non-dispatchable Renewable Electricity Generation

Access to an inexpensive and reliable supply of energy is critical for the success of modern civilisation. Since the beginning of the Industrial Revolution in the mid 18th century, fossil fuels have enabled great advances across many aspects of society, which have increased the standard of living for many. Unfortunately, dwindling supplies and greenhouse gas emissions resulting from their use means that the continued utilisation of these fuels—particularly for electricity generation and transportation—is simply not sustainable. Present-day electricity systems are built around the premise that generation is flexible and controllable, while load—generally speaking—is not. This leads to dispatch models where generation is scheduled to meet load, plus some additional capacity to accommodate forecast errors and potential equipment failure. Many renewable generation technologies, such as wind and solar photovoltaics, are non-dispatchable and cannot be scheduled to produce electricity on-demand. Successfully utilising these energy sources therefore requires flexibility in other parts of the system. Electric Vehicles (EVs) produce no tailpipe emissions, and can be charged at any location with an electricity supply; at home, work, supermarket, or dedicated charging facilities. Because driving times tend to coincide with existing peak electricity demand, EV charging will occur at times of already high electricity demand if not controlled. Fortunately, there is substantial flexibility over the timing of charging, which can be exploited to minimise adverse impacts on electricity grids. Additional benefits are realised when energy is allowed to flow from the vehicle’s battery back into the electricity grid; a concept known as vehicle-to-grid (V2G). Through the development of a simulation based on future energy scenarios in New Zealand, the research presented in this thesis evaluates the extent to which the flexibility of EV charging may be exploited to support high levels of non-dispatchable renewable electricity generation. Several EV charging strategies are introduced and evaluated across a range of metrics with wind penetration levels ranging between 10% and 50% on an annual energy basis. With a V2G-enabled fleet consisting of one million vehicles (25% of New Zealand’s projected light vehicle fleet size in 2030), it is found that EV charg-
Vaccination With Leptospira interrogans PF07598 Gene Family-Encoded Virulence Modifying Proteins Protects Mice From Severe Leptospirosis and Reduces Bacterial Load in the Liver and Kidney

The molecular and cellular pathogenesis of leptospirosis remains poorly understood. Based on comparative bacterial genomics data, we recently identified the hypothetical PF07598 gene family as encoding secreted exotoxins (VM proteins) that mediate cytotoxicity in vitro. To address whether VM proteins mediate in vivo leptospirosis pathogenesis, we tested the hypothesis that VM protein immunization of mice would protect against lethal challenge infection and reduce bacterial load in key target organs. C3H/HeJ mice were immunized with recombinant E. coli-produced, endotoxin-free, leptospiral VM proteins (derived from L. interrogans serovar Lai) in combination with the human-compatible adjuvant, glucopyranoside lipid A/squalene oil-in-water. Mice receiving full length recombinant VM proteins were protected from lethal challenge infection by L. interrogans serovar Canicola and had a 3-4 log10 reduction in bacterial load in the liver and kidney. These experiments show that immunization with recombinant VM proteins prevents leptospirosis clinical pathogenesis and leads to markedly reduced key target organ infection in this animal model. These data support the role of leptospiral VM proteins as virulence factors and suggest the possibility that a VM protein-based, serovar-independent, pan-leptospirosis vaccine may be feasible.
The Correspondence Between Students’ and Teachers’ Views on Teachers’ Emotional Scaffolding Strategies in English Classes in Iran

Academic emotions can play a major role in students’ learning in their English classes. The literature about emotional scaffolding strategies is not widely developed; most of the studies merely focus on negative emotions in EFL contexts. However, in today’s world of varied psychological elements, it is more critical than ever before to scaffold students’ positive emotions to provide more opportunities for their classroom engagement. This study is to explore teachers’ strategies of enhancing students’ positive emotions in English classes in Iran. To investigate the correspondence between students’ and teachers’ views on the effective emotional scaffolding strategies of English teachers, an exploratory sequential mixed-methods design was conducted. First, the researcher conducted semi-structured interviews with 11 high school students of Salami School in Kazeroun to collect in-depth qualitative data. Then, the interview-based Likert scale questionnaires were distributed and completed by 60 high school English teachers in Kazeroun for the quantitative data. According to the teachers’ self-reports, expressing optimism and hope about the students’ future lives and prospective jobs was the most frequently used strategy. There was a significant difference among the teachers’ views on the emotional scaffolding strategies. Moreover, some of the strategies taken into consideration as effective strategies by the students are the least frequently used ones according to the teachers’ reports. The results and findings have several theoretical and practical implications for English teachers in EFL contexts; they might bolster their understanding of appropriate emotional scaffolding strategies preparing them to play a contributing role as great facilitators in English classes.
Nematode microRNAs can Individually Regulate Interferon Regulatory Factor 4 and mTOR in Differentiating T Helper 2 Lymphocytes and Modulate Cytokine Production in Macrophages

Parasitic nematodes are masterful immunomodulators. This class of pathogens has evolved a spectrum of sophisticated strategies to regulate and evade host immune responses, mediated through the release of various molecules. In this context, the release of microRNAs (miRNAs), short post-transcriptional regulators of gene expression, has been of particular interest in the host-parasite interplay. Evidence that parasite-derived miRNAs modulate host innate and adaptive immune responses has become increasingly compelling. However, since miRNAs are usually contained in extracellular vesicles containing other mediators, it is difficult to assign an observed effect on host cells to miRNAs specifically. Here, the effects of some abundantly secreted miRNAs by nematodes used as models of gastrointestinal infections (Heligmosomoides polygyrus bakeri, Trichuris muris and Ascaris suum) were evaluated, addressing the potential of parasite miRNAs to impair in vitro differentiation of two important types of immune cells in the context of helminth infections, Th2 lymphocytes and macrophages. Mimicking a continuous exposure to low concentrations of nematode miRNAs, the interferon gamma signaling, the IL-2/STAT5 signaling, and the mTOR signaling pathways were identified as downregulated by Hpo-miR-71-5p. Interferon regulatory factor 4 (Irf4) was validated as a target of Hpo-miR-71-5p, while Mtor is targeted by Asu-miR-791-3p, abundant in the T. muris secretions. By trend, Hpo-miR-71-5p impacts mildly but consistently on the amounts of inflammatory cytokines in unpolarized macrophages but leads to slightly increased IL-10 level in alternatively activated cells. In addition, our data suggests that transfected miRNAs remain for days in recipient cells, and that Hpo-miR-71-5p can incorporate into mouse Argonaute protein complexes. Nematode miRNAs can impair both innate and adaptive arms of host immunity. Hpo-miR-71-5p in particular, absent in mammals, interacts with host genes and pathways with crucial involvement in anthelmintic immune responses. This report brings new insights into the dynamics of miRNA-driven immunomodulation and highlights putative targeted pathways. Although the absolute repression is subtle, it is expected that the dozens of different miRNAs released by nematodes may have a synergistic effect on surrounding host cells.
Safety Performance of Flashing Yellow Arrow for Protected–Permissive Left-Turn Signal Control in Central Illinois

In 2010, the Illinois Department of Transportation began implementing the flashing yellow arrow (FYA) at intersections operating with protected–permissive left-turn (PPLT) control. Research was conducted to evaluate the safety-effectiveness of FYAs at 86 intersections and 164 approaches in central Illinois. The effectiveness evaluation was performed with 3 years of before-and-after FYA installation crash data and the empirical Bayes method. In the before condition, the left-turn signals operated with a circular green display indicating the permissive interval of PPLT control using a five-section signal head. In the after condition, the FYA replaced the circular green display for the permissive interval of PPLT with a four-section signal head. Supplemental traffic signs were mounted on the mast arm adjacent to the left-turn signal at over half of the FYA installations. The results of the comprehensive safety evaluation of the FYA for PPLT control are presented. Analyses were also performed to assess the effects of the FYA supplemental signs and the effects of the FYA overall on two subsets of at-fault drivers: older drivers (age 65+) and younger drivers (age 16 to 21). The resulting mean crash modification factors for the targeted crash types ranged from 0.589 to 0.714. The findings of this research support the continued use of FYAs for PPLT control to improve safety at signalized intersections in central Illinois.
Goat Breeds Utilization and Productivity of Crossbred Goats in Eastern and Southern Ethiopia and Biophysical Model

The major objectives of this work were to assess the utilization and management condition of crossbred and indigenous goats and to develop a biophysical model for crossbred goats. A survey was made in three districts (Kombolcha, Gursum and Konso) in Eastern and Southern Ethiopia to assess the utilization of these goats. In addition, a three-year data was obtained from Haramaya University to develop the biophysical model. Nearly 92% of the respondents prefer crossbred goats to indigenous goats. These respondents prefer crossbred goats due to the physical, productive, and reproductive features of the crossbred goats compared to the indigenous goats. However, in terms of ability to walk long distance, longevity and disease tolerance, local goats were preferred by most of the farmers than the crossbred goats. For milk and meat production crossbred goats were their first choice than local goats. The most common method of grazing crossbred goats was tethering. About 74% of Kombolcha, 86% of Gursum and 94% of Konso district respondents feed their crossbred goats through tethering. The amount of dry matter (DM) protein supplement was the major factor affecting productivity of crossbred goats. The amount of protein supplement given to the crossbred goats affected longevity, daily milk yield and lactation length. Crossbred had higher milk yield and longer lactation length than indigenous goats but they suffered higher mortality. In the districts an average of 3.17 family labors was used per flock. Most of the crossbred goat owners practiced controlled breeding. Goats’ health care was found to be one of the most important factors that affect the productivity of crossbred goats. Increased investment (expenditure) for medical cases was found to increase the longevity of goats, milk yield and lactation length of the goats. Information generated in this study is believed to guide investment priorities for in goat-keeping families in the country.
Dendritic Cells and Macrophages in the Pathogenesis of Psoriasis

Psoriasis is a chronic inflammatory skin disease characterized by scaly indurated erythema. This disease impairs patients’ quality of life enormously. Pathological findings demonstrate proliferation and abnormal differentiation of keratinocytes and massive infiltration of inflammatory immune cells. The pathogenesis of psoriasis is complicated. Among immune cells, dendritic cells play a pivotal role in the development of psoriasis in both the initiation and the maintenance phases. In addition, it has been indicated that macrophages contribute to the pathogenesis of psoriasis especially in the initiation phase, although studies on macrophages are limited. In this article, we review the roles of dendritic cells and macrophages in the pathogenesis of psoriasis.
LARGE WOODY DEBRIS ACCUMULATIONS IN THE LATE PENNSYLVANIAN TROPICS—EVOLUTIONARY SIGNAL OR TECTONO-CLIMATIC ARCHIVE?

Abstract: By colonizing drylands, plants fundamentally changed continental deposition and, thus, intensified the interaction between life and sediments. Fossil large woody debris in epiclastic strata is a key archive of this environmental turnover, although its interpretation remains challenging due to taphonomic biases. We review voluminous fluvial red-bed successions with sizeable silicified trunks that characterize Middle Pennsylvanian–lower Permian strata of east-central Europe. The stratigraphic occurrence, petrography, architecture of the deposits, and the preservation and nature of the fossil wood are discussed in the context of the tectono-climatic and vegetational evolution of the central-Pangean low latitudes. The log-bearing successions are assigned to five distinct, regionally traceable stratigraphic levels between the middle Moscovian and early Asselian. Up to 20 m long, mostly decorticated trunk fragments occur isolated in more or less feldspathic channel deposits, the architectures and dimensions of which point to large-scale river systems with highly variable discharge. Wood anatomy and floodplain adpression-fossils show that the trunks were derived from cordaitaleans, conifers, and arborescent sphenopsids in more diverse, gymnosperm-dominated dryland floras. The fossil record is biased towards successions formed in large-catchment river systems and, thus, does not accurately document the genuine nature of plant-distribution patterns. Rather, the strata show that large woody debris preservation depended on fluvial style and hydrological regime, hence turning the woody deposits into climate archives. The strata elucidate the climate development in equatorial Pangea, paralleling the acme of the Late Paleozoic Ice Age.
Medical Council of Canada Qualifying Examinations and performance in future practice

The purpose of medical licensing examinations is to protect the public from practitioners who do not have adequate knowledge, skills, and abilities to provide acceptable patient care, and therefore evaluating the validity of these examinations is a matter of accountability. Our objective was to discuss the Medical Council of Canada's Qualifying Examinations (MCCQEs) Part I (QE1) and Part II (QE2) in terms of how well they reflect future performance in practice. We examined the supposition that satisfactory performance on the MCCQEs are important determinants of practice performance and, ultimately, patient outcomes. We examined the literature before the implementation of the QE2 (pre-1992), post QE2 but prior to the implementation of the new Blueprint (1992-2018), and post Blueprint (2018-present). The literature suggests that MCCQE performance is predictive of future physician behaviours, that the relationship between examination performance and outcomes did not attenuate with practice experience, and that associations between examination performance and outcomes made sense clinically. While the evidence suggests the MCC qualifying examinations measure the intended constructs and are predictive of future performance, the validity argument is never complete. As new competency requirements emerge, we will need to develop valid and reliable mechanisms for determining practice readiness in these areas.
Is it Worth Getting a Doctorate?

PhD training is part of the global education competition. The number of doctoral students per 100,000 people is a feature that moves together with the scientific performance of a country. Thus, doctoral training is an important element of a country’s innovation, in which Hungary is lagging behind. We assume that the main reason for the limited amount of doctoral students in Hungary is the low level of individual demand for training which, in turn, is due to the fact that the academic career of doctoral students promises low earnings compared to the placement of university graduates in other spheres. The earnings test and life earnings analysis presented by the study support this assumption: the earnings of those entering academically qualified academic careers are significantly lower than those of graduates working in the corporate sector. Though there are limitations present which impede the generalizability of these results, it does call to attention important trends to be acknowledged in future research.
Epidemiological perspective associated with principal risk factors of Trichinella spiralis infection in pigs and humans in Egypt

Background and Aim: In Egypt, there is a scarcity of recent data on trichinellosis in pigs and humans. Therefore, this study aimed to determine the epidemiological profile and risk factors associated with Trichinella spiralis infection as well as to assess the effectiveness of the trichinoscope and digestion technique in diagnosing trichinellosis. Materials and Methods: Data were collected on 33812 pigs slaughtered during a year at the Al-Basateen abattoir, Cairo Governorate, Egypt. The slaughtered pigs had already been examined by trichinoscope in the abattoir. The diagnostic effectiveness technique was randomly conducted on 170 pork muscle samples, which were examined using the digestion technique. Furthermore, 90 serum samples from high-risk individuals in Qena and Sohag Governorates, Upper Egypt, were analyzed using an enzyme-linked immunosorbent assay. Results: The investigation revealed that the overall prevalence was 1.06% in pigs by trichinoscope. Of the examined 170 samples, 2.35% and 3.35% were found to harbor Trichinella by trichinoscope and artificial digestion, respectively. Trichinella was identified as T. spiralis using a polymerase chain reaction (PCR) technique. A significant relationship was affirmed between the prevalence of trichinellosis and the sex and age of the examined pigs. Likewise, for the first time, there was a considerable seasonal trend in the prevalence of Trichinella with the maximum infection, which was observed during Autumn (1.18%). The prevalence of trichinellosis in humans was 10%, with a significant association with age. Conclusion: Our findings are intended to serve as a starting point for developing effective preventive and control measures for trichinellosis (as application of Hazard Analysis Critical Control Points (HACCP) in pig farms, stop feeding pigs on garbage as well as, preventing illegal slaughter of pigs outside the slaughterhouses). It also fortifies the establishment of the digestion technique because of its high specificity and sensitivity, although it is difficult to apply to a large number of samples.