Miracle-dz/UniFAR-FTUs huggingface dataset:
The changes in the dominance hierarchy over time of a complete field‐captured colony of Cryptomys hottentotus hottentotus

The common mole-rat, Cryptomys h. hottentotus, is a social subterranean rodent occurring in colonies in which one female and one to three males are involved in reproduction and the remaining colony members are non-reproductive. Within each sex the reproductive animals are usually the largest and most dominant animals. The dominance hierarchy amongst a field-captured colony was linear (h = 0.95, calculated from Landau's linearity index) soon after capture. The non-reproductive females were ranked low in the dominance hierarchy; many were subordinate to non-reproductive males. The order of capture of mole-rats was not related to the position in the dominance hierarchy. The hierarchy became non-linear (h = 0.56) after six months in captivity during which two juvenile animals became adult. The breakdown in the hierarchy may result from the lack of opportunity in captivity for animals to disperse and establish satellite colonies, or from colony members becoming co-dominant in the hierarchy as a result of a rise in rank by young animals. Dominant mole-rats are involved in a greater proportion of interactive behaviours than subordinates. Popularity studies show that females tend to be more popular animals than males. The largest reproductive male was the least popular animal in the first study, whereas a beta male was the least popular animal in the second study period. The reproductive female was the most popular in both periods.
Carotenoids in Solonocera indica and Aristeus alcocki, Deep-Sea Shrimp from Indian Waters

ABSTRACT Carotenoids are the major pigments responsible for the color of crustaceans like shrimp. Quantitative and qualitative distribution of carotenoids in different body components of deep-sea shrimp Solonocera indica and Aristeus alcocki, from Indian waters were assessed. The yield of waste (head and carapace) from processing of these shrimp ranged from 62.6–65.6%. Carotenoid content was higher in A. alcocki and the highest total carotenoid content of 185.3 µg/g was observed in head of A. alcocki. Astaxanthin and its mono- and diesters (63.5–92.2%) were the major carotenoids in both the species of shrimp and the levels of esterified astaxanthin were higher than the free form of astaxanthin. The levels of astaxanthin esters were higher (61.7–70.8%) in A. alcocki compared to S. indica (43.8–58.4%). Highest unsaturated fatty acid content (60.5%) was observed in the carotenoid extract from head of A. alcocki, and the highest saturated fatty acid content (83.1%) was observed in the carotenoid extract from the carapace S. indica.
Changes in Thyroid and Glycemic Status and Food Intake in Children with Excess Weight Who Were Submitted for a Multi-Component School Intervention for 16 Months

We investigated if children with excess weight who submitted to two types of intervention at school for 16 months showed improvements in thyroid and glycemic function and food intake. Children (8–11 years) with a body mass index-for-age (BMI/A) of ≥1 Z score were divided into two groups: Treatment 1 (n = 73) involved motivation to adopt healthier lifestyle; Treatment 2 (n = 103) involved performing weekly nutritional education, motivational, and physical activities at school. A semi-quantitative food frequency questionnaire was used. The delta BMI/A were similar after 16 months; Treatment 1 showed higher decrease in thyroid-stimulating hormone (TSH; median (range)): −0.45 (−3.19 to 2.17) and 0.06 (−4.57 to 1.63) mIU/L, p = 0.001), FreeT3 (−0.46 (−2.92 to 1.54) and −0.15 (−2.46 to 1.38) pmol/L, p = 0.038), and FreeT4 −1.41 (−6.18 to 3.47) and −0.90 (−4.89 to 2.96) pmol/L, p = 0.018), followed by decrease in energy intake (7304 (6806 to 7840) and 8267 (7739 to 8832) kJ, Ptreatment = 0.439, Ptime <0.001, interaction group–time p < 0.001), macronutrients and sugar. A positive correlation between FreeT3 and BMI/A, and a negative correlation with FreeT4 and insulin were found at baseline (r 0.212, p < 0.01; r −0.155, p < 0.01, respectively) and follow-up (r 0.222, p < 0.01; r −0.221, p < 0.01). The decrease in overall diet and particularly sugar intake was accompanied by a greater reduction in TSH and FreeT3 in Treatment 1, demonstrating the impact of dietary intake on thyroid function.
CAMSAP3 facilitates basal body polarity and the formation of the central pair of microtubules in motile cilia

Significance Cilia are composed of hundreds of proteins whose identities and functions are far from being completely understood. In this study, we determined that calmodulin-regulated spectrin-associated protein 3 (CAMSAP3) plays an important role for the function of motile cilia in multiciliated cells (MCCs). Global knockdown of CAMSAP3 protein expression in mice resulted in defects in ciliary structures, polarity, and synchronized beating in MCCs. These animals also displayed signs and symptoms reminiscent of primary ciliary dyskinesia (PCD), including a mild form of hydrocephalus, subfertility, and impaired mucociliary clearance that leads to hyposmia, anosmia, rhinosinusitis, and otitis media. Functional characterization of CAMSAP3 enriches our understanding of the molecular mechanisms underlying the generation and function of motile cilia in MCCs. Synchronized beating of cilia on multiciliated cells (MCCs) generates a directional flow of mucus across epithelia. This motility requires a “9 + 2” microtubule (MT) configuration in axonemes and the unidirectional array of basal bodies of cilia on the MCCs. However, it is not fully understood what components are needed for central MT-pair assembly as they are not continuous with basal bodies in contrast to the nine outer MT doublets. In this study, we discovered that a homozygous knockdown mouse model for MT minus-end regulator calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), Camsap3tm1a/tm1a, exhibited multiple phenotypes, some of which are typical of primary ciliary dyskinesia (PCD), a condition caused by motile cilia defects. Anatomical examination of Camsap3tm1a/tm1a mice revealed severe nasal airway blockage and abnormal ciliary morphologies in nasal MCCs. MCCs from different tissues exhibited defective synchronized beating and ineffective generation of directional flow likely underlying the PCD-like phenotypes. In normal mice, CAMSAP3 localized to the base of axonemes and at the basal bodies in MCCs. However, in Camsap3tm1a/tm1a, MCCs lacked CAMSAP3 at the ciliary base. Importantly, the central MT pairs were missing in the majority of cilia, and the polarity of the basal bodies was disorganized. These phenotypes were further confirmed in MCCs of Xenopus embryos when CAMSAP3 expression was knocked down by morpholino injection. Taken together, we identified CAMSAP3 as being important for the formation of central MT pairs, proper orientation of basal bodies, and synchronized beating of motile cilia.
Effect of alfalfa grass silage dry matter content on ruminal digestion and milk production in lactating dairy cows

In trial one, seven lactating Holstein cows fitted with rumen cannulae were used in a replicated 3 × 3 Latin square design to investigate the effect of feeding alfalfa/grass ensiled at three dry matter (DM) levels (27.5, 46.1 and 56.4%) on the rate and extent of protein degradation. Cows were fed a total mixed ration of 70% alfalfa/grass silage, at one of three DM levels, and 30% concentrate (high moisture corn and mineral) (DM basis). Silage samples were incubated in sacco in the rumen for up to 48 h to model disappearance of DM, neutral detergent fibre (NDF) and nitrogen (N). Passage rates of silage, using Cr-mordanted NDF, were determined for small and total particulate fractions. Rates of DM, NDF and N disappearance, as well as rates of passage were unaffected by silage DM content. The 27% DM silage contained a larger soluble N fraction, but a smaller fraction of potentially digestible N than either the 46 or 56% DM silages. Estimates of effective protein degradability corrected for microbial contamin.
The “Warrior” system: a new useful emergency simulator to train clinical pharmacists in emergency medicine

Abstract Clinical pharmacists are indispensable providers of emergency medical services. However, the training of Chinese clinical pharmacists in medical emergency skills is apparently insufficient. The current study aimed to evaluate the effect of the “Warrior” emergency simulator application in the emergency medical education of clinical pharmacy students (CP students). The “Warrior” system, which contains a pharmacokinetics/pharmacodynamics-linked model and a drug database, was successfully employed to train CP students and improve their capability to deal with various medical emergency situations. Both an objective (in-class) test and the subjective Dundee Ready Education Environment Measure (DREEM) were administered to 20 CP students, randomly divided into an intervention group and a control group, to estimate the teaching effect of the “Warrior” system. The scores of CP students from the intervention group were significantly higher (P < .01) in the in-class test than the scores of students from the control group due to the diverse situational teaching using the “Warrior” system. The results of the DREEM showed that CP students from the intervention group obtained considerably better (P < .01) marks for “students’ perceptions of learning” and “students’ perceptions of atmosphere” than those from the control group. Furthermore, the intervention group scored much higher (P < .01) than the control group on the total DREEM. The “Warrior” system provides an excellent training path for clinical pharmacists that supplies a more realistic clinical simulation experience and significantly improves the teaching effect. The “Warrior” system exhibits high potential for future development in emergency medical education.
Outcomes of simultaneous pancreas and kidney transplants based on preemptive transplant compared to those who were on dialysis before transplant – a retrospective study

Among kidney transplant recipients, the duration of pretransplant dialysis is significantly associated with worse post‐transplant outcomes. However, data on the outcomes of preemptive simultaneous pancreas and kidney (SPK) are limited. We analyzed primary SPK recipients transplanted between January 2000 and December 2017. Patients were divided into two groups based on pretransplant dialysis history of preemptive versus non‐preemptive. Patient and survival of grafts were outcomes of interest. Of the 644 recipients, 174 (27%) were preemptive and 470 (73%) were not. Most of the baseline characteristics were similar between the groups. In the univariable analysis, the non‐preemptive transplant was associated with 54% increased risk for kidney death‐censored graft failure (DCGF; HR: 1.54; 95% CI: 1.01–2.35; P = 0.05). There was a 29% increased risk after adjustment for confounding factors (HR: 1.29; 95% CI: 0.83–2.02; P = 0.26), although this association was not statistically significant. Similarly, there was a 16% increased risk of pancreas DCGF in univariable analysis and 1% after adjustment, which was also not statistically significant. When outcomes were based on the duration of pretransplant dialysis, the duration was not associated with either patient survival or survival of either graft in K‐M analysis. In SPK recipients, with pretransplant dialysis history, there was a tendency toward inferior graft survival, mainly for the kidney more than the pancreas.
RANKL inhibition improves muscle strength and insulin sensitivity and restores bone mass.

Receptor activator of NF-κB ligand (RANKL) activates, while osteoprotegerin (OPG) inhibits, osteoclastogenesis. A neutralizing Ab against RANKL, denosumab, improves bone strength in osteoporosis. OPG also improves muscle strength in mouse models of Duchenne’s muscular dystrophy (mdx) and denervation-induced atrophy, but its role and mechanisms of action on muscle weakness in other conditions remain to be investigated. We investigated the effects of RANKL inhibitors on muscle in osteoporotic women and mice that either overexpress RANKL (HuRANKLTg+), or lack Pparb and concomitantly develop sarcopenia (Pparb–/–). In women, taking denosumab for more than 3 years improved appendicular lean mass and handgrip strength compared with no treatment, whereas bisphosphonate did not. HuRANKLTg+ mice displayed lower limb force and maximal speed, while their leg muscle mass was diminished, with a lower number of type I and II fibers. Both OPG and denosumab increased limb force proportionally to the increase in muscle mass. They markedly improved muscle insulin sensitivity and glucose uptake, and decreased antimyogenic and inflammatory gene expression in muscle, such as myostatin and protein tyrosine phosphatase receptor-γ. Similarly, in Pparb–/–, OPG increased muscle volume and force while also normalizing insulin signaling and higher expression of inflammatory genes in skeletal muscle. In conclusion, RANKL deteriorates while its inhibitors improve muscle strength and insulin sensitivity in osteoporotic mice and humans. Hence, denosumab could represent a novel [. ] Research Article Bone biology Muscle biology
Fewer Than Half Of US Mental Health Treatment Facilities Provide Services For Children With Autism Spectrum Disorder.

Despite estimates of rising prevalence of children with autism spectrum disorder (ASD) in the US, there is little research on the availability of behavioral health care services for this vulnerable population. To fill the gap, we surveyed 8,184 mental health treatment facilities in the contiguous US. As of August 2019, 43.0 percent of facilities reported providing behavioral health care for children with ASD, and 36.6 percent were accepting such children as new patients. Only 12.7 percent reported having a clinician with specialized training, and 4.3 percent reported having a specialized treatment program. Multivariable regression results indicated that there was poorer access to specialized ASD care in rural and lower-income communities. New policies are needed to increase the availability of behavioral health care for children with ASD.
The impact of intimate partner violence on the trajectory of perinatal depression: a cohort study in a Chinese sample

Abstract Aims Intimate partner violence (IPV) is an important risk factor for perinatal depression (PND). But IPV's impact on the natural prognosis of PND symptoms is not well understood. We tested two hypotheses: (1) pregnant women with IPV experiences will exhibit more severe PND symptoms than women without IPV experience; (2) IPV experience will impede the recovery prognosis of PND. We also explored the contribution of IPV to PND comparing with other risk factors. Method The sample is comprised of 813 pregnant women followed through perinatal period in Hunan, China. We assessed IPV experience using items from the Short Form of the Revised Conflict Tactics Scale (CTS2S), and PND symptoms via the Edinburgh Postnatal Depression Scale (EPSD). We conducted Linear Mixed-effects Model to compare the trajectories of PND symptoms between victims and non-victims and a multistage Generalised Estimating Equations Model to explore salient factors on the trajectory of PND symptoms. Results There were 90 participants (11.07%) who reported IPV experience in the past 12 months. With respect to physical, psychological and sexual violence, the prevalence was 4.55% (37/813), 9.23% (75/813) and 2.34% (19/813). Victims reported more severe PND symptoms (t = 5.30, p < 0.01) and slower decreasing slope of trajectories (t = 28.89, p < 0.01). The PND trajectory was associated with IPV experience (OR = 3.78; 95% CI 1.39–10.26), social support (OR = 0.93; 95% CI 0.88–0.97), positive coping strategies (OR = 0.85; 95% CI 0.80–0.91), negative coping strategies (OR = 1.25; 95% CI 1.14–1.37) and monthly income of $0.15–$298.36 (compared to no income, OR = 0.0075; 95% CI 0.00052–0.11). Conclusions The findings suggest the reported prevalence of IPV is lower in Hunan than most of the previous studies during perinatal period in other provinces of China, and IPV victimisation is associated with increased severity and slowed prognosis of PND symptoms. Future studies that screen for victimisation and establish its explicit mechanism to the poorer prognosis of PND symptoms would benefit the prevention and treatment of PND.
Discovery of New Apoptosis-Inducing Agents for Breast Cancer Based on Ethyl 2-Amino-4,5,6,7-Tetra Hydrobenzo[b]Thiophene-3-Carboxylate: Synthesis, In Vitro, and In Vivo Activity Evaluation

A multicomponent synthesis was empolyed for the synthesis of ethyl 2-amino-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylate 1. An interesting cyclization was obtained when the amino-ester 1 reacted with ethyl isothiocyanate to give the benzo[4,5]thieno[2,3-d][1,3]thiazin-4-one 3. Acylation of the amino-ester 1 with chloroacetyl chloride in DCM and Et3N afforded the acylated ester 4. The amino-ester 1 was cyclized to benzo[4,5]thieno[2,3-d]pyrimidin-4(3H)-one 8, which was reacted with some alkylating agents leading to alkylation at nitrogen 9–13. Hydrazide 14 was utilized as a synthon for the synthesis of the derivatives 15–19. Chloro-thieno[2,3-d]pyrimidine 20 was synthesized and reacted with the hydrazine hydrate to afford the hydrazino derivative 21, which was used as a scaffold for getting the derivatives 22–28. Nucleophilic substitution reactions were used for getting the compounds 29–35 from chloro-thieno[2,3-d]pyrimidine 20. In the way of anticancer therapeutics development, the requisite compounds were assessed for their cytotoxicity in vitro against MCF-7 and HepG-2 cancer cell lines. Twelve compounds showed an interesting antiproliferative potential with IC50 from 23.2 to 95.9 µM. The flow cytometric analysis results showed that hit 4 induces the apoptosis in MCF-7 cells with a significant 26.86% reduction in cell viability. The in vivo study revealed a significant decrease in the solid tumor mass (26.6%) upon treatment with compound 4. Moreover, in silico study as an agonist for inhibitors of JAK2 and prediction study determined their binding energies and predicted their physicochemical properties and drug-likeness scores.
Effect of partial replacement of meat by carrot on physicochemical properties and fatty acid profile of turkey fresh sausages: A chemometric approach.

BACKGROUND In this study, the modification of physicochemical characteristics and fatty acid composition of turkey fresh sausages manufactured including carrot (Daucus carota L.) as vegetable source (i.e., 10%, 20% and 30%, w/w) were evaluated. RESULTS The results were compared with sausages used as control (i.e., containing 100% turkey meat, w/w). Overall, significant differences were detected for all the evaluated attributes. The inclusion of 20% and 30% carrot (w/w) to sausages resulted in a reduction of the lipid content (being 5.42 and 5.26 g/100g, respectively) followed by a reduced energy value (-7.6%). Besides, the colour parameters were strongly affected by carrot inclusion, recording a significant increase of both redness and yellowness. In addition, an average reduction of Na content (-47.4%) was observed when compared experimental carrot vs. control sausages. Interestingly, a significant reduction of n-6/n-3 fatty acids ratio was also detected. OPLS-DA multivariate statistics carried out on the studied parameters suggested that the inclusion of carrot provided completely different nutritional and physicochemical profiles, with colour parameters possessing the highest discrimination potential. CONCLUSION Therefore, including carrot in turkey sausages manufacturing could represent a valuable tool to design healthier meat products. This article is protected by copyright. All rights reserved.
Leaks in the epithelial barrier caused by spontaneous and TNF‐α‐induced single‐cell apoptosis

Current opinion assumes epithelial integrity during spontaneous apoptotic cell death. We measured, for the first time, the local conductances associated with apoptoses and show leaks of up to 280 nS (mean 48 ± 19 nS) in human intestinal epithelium. The results disprove the dogma that isolated cell apoptosis occurs without affecting the epithelial cell permeability barrier. After induction by tumor necrosis factor α (TNF‐α) the apoptotic leaks were dramatically enhanced: not only was the frequency increased by threefold, but the mean conductance also increased by 12‐fold (597±98 nS). Thus, apoptosis accounted for about half (56%) of the TNF‐α‐induced permeability increase whereas the other half was caused by degradation of tight junctions in nonapoptotic areas. Hence, spontaneous and induced apoptosis hollow out the intestinal barrier and may facilitate loss of solutes and uptake of noxious agents. —Gitter, A. H. , Bendfeldt, K. , Schulzke, J.‐D. , Fromm, M. Leaks in the epithelial barrier caused by spontaneous and TNF‐α‐induced single‐cell apoptosis. FASEB J. 14, 1749–1753 (2000)
Metabolomic Profiling for Diagnosis and Prognostication in Surgery: A Scoping Review.

OBJECTIVE This review assimilates and critically evaluates available literature regarding the use of metabolomic profiling in surgical decision-making. BACKGROUND Metabolomic profiling is performed by nuclear magnetic resonance spectroscopy or mass spectrometry of biofluids and tissues to quantify biomarkers (ie, sugars, amino acids, and lipids), producing diagnostic and prognostic information that has been applied among patients with cardiovascular disease, inflammatory bowel disease, cancer, and solid organ transplants. METHODS PubMed was searched from 1995 to 2019 to identify studies investigating metabolomic profiling of surgical patients. Articles were included and assimilated into relevant categories per PRISMA-ScR guidelines. Results were summarized with descriptive analytical methods. RESULTS Forty-seven studies were included, most of which were retrospective studies with small sample sizes using various combinations of analytic techniques and types of biofluids and tissues. Results suggest that metabolomic profiling has the potential to effectively screen for surgical diseases, suggest diagnoses, and predict outcomes such as postoperative complications and disease recurrence. Major barriers to clinical adoption include a lack of high-level evidence from prospective studies, heterogeneity in study design regarding tissue and biofluid procurement and analytical methods, and the absence of large, multicenter metabolome databases to facilitate systematic investigation of the efficacy, reproducibility, and generalizability of metabolomic profiling diagnoses and prognoses. CONCLUSIONS Metabolomic profiling research would benefit from standardization of study design and analytic approaches. As technologies improve and knowledge garnered from research accumulates, metabolomic profiling has the potential to provide personalized diagnostic and prognostic information to support surgical decision-making from preoperative to postdischarge phases of care.
Seasonal characteristics of the surface mixed layer in the Australasian region: implications for primary production regimes and biogeography

The seasonal cycle of physical, chemical, and biological properties of the surface ocean mixed layer in the Australasian region (0 to 50°S, 90 to 180°E) were described on the basis of a range of data products, some of which are described for the first time. They include seasonal fields of temperature, salinity, mixed layer depth, nitrate, phosphate and silicate from the CSIRO Atlas of Regional Seas (CARS), as well as estimates of chlorophyll from SeaWiFS ocean colour data, and a range of supplementary information taken from published studies. Seasonal chlorophyll cycles were interpreted within the context of variability in nutrient concentrations and mixed layer depths. This interpretation included a biogeographical description, which was compared with related regional and global products. Such descriptions provide a baseline for future investigations of interannual variability and long-term trends in mixed layer properties, as well as contributing to the development of spatial frameworks for management of the region’s resources.