Miracle-dz/UniFAR-FTUs huggingface dataset:
Adoption of Sustainable Agriculture Practices in Banana Farm Production: A Study from the Sindh Region of Pakistan

The aim of this study was to highlight the importance of socioeconomic and psychosocial factors in the adoption of sustainable agricultural practices (SAPs) in banana farm production. To this end, data from 300 randomly selected farm households from Pakistan were collected through a structured self-report questionnaire. Using logistic regression (LR) and structural equation modeling (SEM), socioeconomic and psychosocial effects were evaluated. The results show that economic status, watching agricultural training programs, newspaper and radio awareness campaigns, participation in extension programs, perceptions of sustainable agriculture and the feasibility of SAPs were significant factors in farmers’ adoption of sustainable agriculture practices. Also, consistent with the theory of planned behavior (TPB), all its dimensions (attitude, subjective norms and perceived behavioral control) affected the adoption of SAPs. This finding highlights the importance of socioeconomic and psychosocial factors in promoting sustainable agricultural practice among banana production farmers. This is the first study which attempts to provide empirical evidence using a robust procedure (two models—LR and SEM). The practical implication is that, when socioeconomic and psychosocial factors are well supported by satisfactory policy measures, SAP adoption is more than likely, which eventually increases farmers’ adaptive capacity to the changing environment. Ultimately, this leads to sustainable banana production, which has great potential to contribute towards poverty eradication.
Changes in self-reported and observed parenting following a randomized control trial of parent-child interaction therapy for the treatment of preschool depression.

BACKGROUND Parenting in early childhood exerts substantial influence over children's emotional health and development. Using data from a randomized controlled trial of a novel treatment for early childhood depression, Parent-Child Interaction Therapy Emotion Development (PCIT-ED), we explored two broad dimensions of parenting (behavior and affect) to determine whether any changes could be detected following treatment when compared to those in a waitlist control condition. METHOD 229 caregiver-child dyads, 114 randomly assigned to PCIT-ED for preschool-onset depression, and 115 assigned to a waitlist completed two structured interaction tasks at baseline and post-treatment. Interactions were later coded by observer's blind to diagnostic and treatment status. RESULTS Greater reductions were found in self-reported negative parenting behaviors and observed negative affect and greater increases in self-reported positive parenting behaviors and observed positive affect among the caregivers in the treatment group. Increases in the overall positivity of the observed interactional style of caregivers, but no observed parenting behavior change was found following treatment. Discrepancies between self-reported and observed parenting were greater among caregivers on the waitlist. CONCLUSIONS Following PCIT-ED treatment, caregivers self-reported improvements in parenting practices and declines in punitive practices along with observed increases in positive affect and decreases in negative affect when interacting with their child. Moreover, coherence between self-reported and observed parenting was higher in the treatment group. These findings highlight the efficacy of PCIT-ED in improving parenting behaviors and the need to use multiple methods to assess parenting in treatment studies.
Nutrient and food group intakes and skeletal muscle index in the Japanese elderly: a cross-sectional analysis of the NHNS 2017

Abstract Objective: To examine nutrient and food intakes according to the levels of skeletal muscle mass index (SMI) in the elderly. Design: Cross-sectional study. Setting: Data were derived from the 2017 National Health and Nutrition Survey in Japan. SMI was calculated by dividing appendicular skeletal muscle (or lean) mass (kg) by height squared (m2). We calculated the multivariable-adjusted means of individuals’ dietary intake. Dietary intake of energy, nutrients and food categories was assessed by examining dietary records using a semi-weighed method and compared according to the sex-specific quartiles of SMI. Participants: Men and women aged ≥60 years. Results: Among 797 men and 969 women, individuals with a higher SMI consumed more energy and more nutrients than did those with a lower SMI after adjusting for age, lifestyle and physical activity factors. After further adjusting for energy intake, total dietary fibre, vitamin A, vitamin B6, K, Fe and Cu were positively associated with higher SMI in men (P for trend < 0·05). For food categories, men with a higher SMI consumed more vegetables and meats, but the associations were attenuated after adjustment for energy and remained significant for vegetable only (P for trend = 0·018). Conclusions: Japanese elderly people with a higher SMI consumed more energy and nutrients and more vegetables than did those with a lower SMI. This finding shows that diet is important in preventing muscle loss among the elderly in an ageing society.
Partners’ Withdrawal When Actors Behave Destructively: Implications for Perceptions of Partners’ Responsiveness and Relationship Satisfaction

Growing evidence indicates that whether critical and hostile behavior harms relationships depends on how partners respond. The current studies test a key behavioral indicator of partners’ responsiveness by examining whether partners’ withdrawal when actors exhibit negative-direct behavior predicts within-person and longitudinal declines in perceived partner responsiveness and relationship satisfaction. Test of Actors’ negative-direct × Partners’ withdrawal interactions indicated that partners’ withdrawal in the context of actors’ negative-direct behavior when targeted for change during conflict discussions (Study 1, N = 162 dyads) and during daily interactions (Study 2, N = 151 dyads) predicted lower perceived partner responsiveness and relationship satisfaction. This Actor × Partner effect did not emerge when actors were pushing for change during conflict (Study 1) and was more consistent predicting perceived partner responsiveness. These results illustrate the importance of Actor × Partner effects and indicate that actors’ own destructive behavior provides an important context to diagnose partners’ responsiveness.
Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells

There is an ample epidemiological evidence to support the role of environmental contaminants such as bisphenol A (BPA) in breast cancer development but the molecular mechanisms of their action are still not fully understood. Therefore, we sought to analyze the effects of three common contaminants (BPA; 4-tert-octylphenol, OP; hexabromocyclododecane, HBCD) on mammary epithelial cell (HME1) and MCF7 breast cancer cell line. We also supplied some data on methoxychlor, MXC; 4-nonylphenol, NP; and 2-amino-1-methyl-6-phenylimidazo [4–b] pyridine, PhIP. We focused on testing the prolonged (two months) exposure to low nano-molar concentrations (0.0015–0.0048 nM) presumed to be oncogenic and found that they induced DNA damage (evidenced by upregulation of pH2A.X, pCHK1, pCHK2, p-P53) and disrupted the cell cycle. Some agents induced epigenetic (methylation) changes of tumor suppressor genes TIMP3, CHFR, ESR1, IGSF4, CDH13, and GSTP1. Obviously, the accumulation of these molecular alterations is an essential base for cancer development. Consistent with this, we observed that these agents increased cellular invasiveness through collagen. Cellular abilities to form colonies in soft agar were increased for MCF7. Toxic agents induced phosphorylation of protein kinase such as EGFR, CREB, STAT6, c-Jun, STAT3, HSP6, HSP27, AMPKα1, FAK, p53, GSK-3α/β, and P70S6 in HME1. Most of these proteins are involved in potential oncogenic pathways. Overall, these data clarify the molecular alterations that can be induced by some common environmental contaminants in mammary epithelial cells which could be a foundation to understand environmental carcinogenesis.
CHEMICAL CHARACTERIZATION OF BEECH CONDENSATE

ABSTRACT Beech (Fagus sylvatica L.) condensate from a steaming operation was collected and analyzed. The condensate exhibited a low pH, and contained carbohydrates and phenolic compounds. A total of five phenolic acids and aldehydes (syringic acid, vanillic acid, p-hydroxybenzoic acid, syringaldehyde, vanillin) were identified. TLC of the carbohydrates showed the presence of glucose, galactose, xylose, mannose and/or arabinose in the condensate. Fructose, saccharose, raffinose and stachyose were also identified. The COD, BOD5, pH, conductivity and suspended solids (TSS) were determined. The chemical oxygen demand (COD) in the beech condensate varied from 2016 to 1393O mg/L. This value is two to three fold higher than the corresponding biological oxygen demand (BOD5).
Reduction of secondary transmission of SARS-CoV-2 in households by face mask use, disinfection and social distancing: a cohort study in Beijing, China

Introduction Transmission of COVID-19 within families and close contacts accounts for the majority of epidemic growth. Community mask wearing, hand washing and social distancing are thought to be effective but there is little evidence to inform or support community members on COVID-19 risk reduction within families. Methods A retrospective cohort study of 335 people in 124 families and with at least one laboratory confirmed COVID-19 case was conducted from 28 February to 27 March 2020, in Beijing, China. The outcome of interest was secondary transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within the family. Characteristics and practices of primary cases, of well family contacts and household hygiene practices were analysed as predictors of secondary transmission. Results The secondary attack rate in families was 23.0% (77/335). Face mask use by the primary case and family contacts before the primary case developed symptoms was 79% effective in reducing transmission (OR=0.21, 95% CI 0.06 to 0.79). Daily use of chlorine or ethanol based disinfectant in households was 77% effective (OR=0.23, 95% CI 0.07 to 0.84). Wearing a mask after illness onset of the primary case was not significantly protective. The risk of household transmission was 18 times higher with frequent daily close contact with the primary case (OR=18.26, 95% CI 3.93 to 84.79), and four times higher if the primary case had diarrhoea (OR=4.10, 95% CI 1.08 to 15.60). Household crowding was not significant. Conclusion The study confirms the highest risk of transmission prior to symptom onset, and provides the first evidence of the effectiveness of mask use, disinfection and social distancing in preventing COVID-19. We also found evidence of faecal transmission. This can inform guidelines for community prevention in settings of intense COVID-19 epidemics.
Cellulosic biomass feedstocks and logistics for ethanol production

The economic competitiveness of cellulosic ethanol production is highly dependent on feedstock cost, which constitutes 35–50% of the total ethanol production cost, depending on various geographical factors and the types of systems used for harvesting, collecting, preprocessing, transporting, and handling the material. Consequently, as the deployment of cellulosic ethanol biorefineries approaches, feedstock cost and availability are the driving factors that influence pioneer biorefinery locations and will largely control the rate at which this industry grows. Initial scenarios were postulated to develop a pioneer dry feedstock supply system design case as a demonstration of the current state of technology. Based on this pioneer design, advanced scenarios were developed to determine key cost barriers, needed supply system improvements, and technology advancements to achieve government and private sector cost targets. Analysis of the pioneer supply system resulted in a delivered feedstock cost to the throat of the pretreatment reactor of $37.00 per dry tonne (2002 $). Pioneer supply systems will start by using current infrastructure and technologies and be individually designed for biorefineries using specific feedstock types and varieties based on local geographic conditions. As the industry develops and cost barriers are addressed, the supply systems will incorporate advanced technologies that will eliminate downstream diversity and provide a uniform, tailored feedstock for multiple biorefineries located in different regions. Published in 2007 by John Wiley & Sons, Ltd.
Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass

The strength of bone depends on bone quantity and quality. Osteocalcin (Ocn) is the most abundant noncollagenous protein in bone and is produced by osteoblasts. It has been previously claimed that Ocn inhibits bone formation and also functions as a hormone to regulate insulin secretion in the pancreas, testosterone synthesis in the testes, and muscle mass. We generated Ocn-deficient (Ocn–/–) mice by deleting Bglap and Bglap2. Analysis of Ocn–/–mice revealed that Ocn is not involved in the regulation of bone quantity, glucose metabolism, testosterone synthesis, or muscle mass. The orientation degree of collagen fibrils and size of biological apatite (BAp) crystallites in the c-axis were normal in the Ocn–/–bone. However, the crystallographic orientation of the BAp c-axis, which is normally parallel to collagen fibrils, was severely disrupted, resulting in reduced bone strength. These results demonstrate that Ocn is required for bone quality and strength by adjusting the alignment of BAp crystallites parallel to collagen fibrils; but it does not function as a hormone.
Within-channel flows promote spawning and recruitment of golden perch, Macquaria ambigua ambigua – implications for environmental flow management in the River Murray, Australia

Restoring fish populations in regulated rivers requires an understanding of relationships between hydrology and population dynamics. In the present study, spawning and recruitment of golden perch, Macquaria ambigua ambigua, were investigated in relation to flow in the regulated lower River Murray. All life stages were sampled in three successive years, with peak flows of 8500 (2004–05), 15 000 (2005–06) and 7000 ML day–1 (2006–07). Larvae occurred only in November/December 2005, and young-of-year fish only in early 2006. Counts of daily increments in otolith microstructure indicated spawning in late October/early November 2005. Back-calculated birth years for adults, derived from otoliths and compared with the hydrograph for the preceding 25 years, revealed the dominance of three year classes spawned in association with increased discharge in 2000, 1998 and 1996. In 2007, an additional year class of 1-year-old fish appeared, following spawning in 2005. In each case, strong recruitment followed spring–summer spawning, when peak flows were >14 000 ML day–1 and water temperatures would have exceeded 20°C. Restoration of within-channel flows of 15–25 000 ML day–1 from late spring through summer would promote spawning and recruitment and improve the resilience of golden perch populations in the lower Murray.
Diabetes in Pregnancy and Risk of Antepartum Depression: A Systematic Review and Meta-Analysis of Cohort Studies

Previous literature has reported that patients with diabetes in pregnancy (DIP) are at risk of developing antepartum depression but the results have been inconsistent in cohort studies. We conducted a systematic review and performed a meta-analysis to quantify the association between DIP and risk of antepartum depression in cohort studies. Medline, Cinahl, and PubMed databases were searched for studies investigating DIP involving pregnant women with pre-existing diabetes and gestational diabetes mellitus and their risk of antepartum depression that were published in journals from inception to 27 December 2019. We derived the summary estimates using a random-effects model and reported the findings as pooled relative risks (RR) and confidence interval (CI). Publication bias was assessed using a funnel plot and was quantified by Egger and Begg’s tests. Ten studies, involving 71,036 pregnant women were included in this meta-analysis. The pooled RR to develop antepartum depression was (RR = 1.430, 95% CI: 1.251–1.636) among women with gestational diabetes mellitus. Combining pregnant women with pre-existing diabetes mellitus and gestational diabetes mellitus, they had a significant increased risk of developing antepartum depression (RR = 1.431, 95% CI: 1.205–1.699) compared with those without it. In comparison, we found no association between pre-existing diabetes mellitus in pregnancy (RR = 1.300, 95% CI: 0.736–2.297) and the risk of developing antepartum depression. This study has a few limitations: first, different questionnaire and cut-off points were used in evaluation of depression across the studies. Second, there was a lack of data on history of depression prior to pregnancy, which lead to confounding bias that could not be solved by this meta-analysis. Third, data were dominated by studies in Western countries; this is due to the studies from Eastern countries failing to meet our inclusion criteria for statistical analysis. Women with gestational diabetes mellitus have an increased risk of developing antepartum depression compared to those without the disease. Therefore, more attention on the mental health status should be given on pregnant women diagnosed with pre-existing diabetes mellitus and gestational diabetes mellitus.
PI(4,5)P2-dependent regulation of exocytosis by amisyn, the vertebrate-specific competitor of synaptobrevin 2

Significance Exocytosis is of vital importance for life, as it allows cells to communicate with each other and with their environment. This dynamic process is highly regulated with a set of both positive and negative regulators. While promotors of exocytosis are well studied, negative regulators are poorly understood. We discovered that a small SNARE protein amisyn (STXBP6) acts as a vertebrate-specific competitor of synaptobrevin-2, a key player in exocytosis. Amisyn contains an N-terminal pleckstrin homology domain that mediates its transient association with the plasma membrane by binding to phospholipid PI(4,5)P2. Both the pleckstrin homology and SNARE domains are needed to inhibit exocytosis. Understanding the mechanisms by which amisyn regulates exocytosis will advance our comprehension of pathological processes in diabetes and autism. The functions of nervous and neuroendocrine systems rely on fast and tightly regulated release of neurotransmitters stored in secretory vesicles through SNARE-mediated exocytosis. Few proteins, including tomosyn (STXBP5) and amisyn (STXBP6), were proposed to negatively regulate exocytosis. Little is known about amisyn, a 24-kDa brain-enriched protein with a SNARE motif. We report here that full-length amisyn forms a stable SNARE complex with syntaxin-1 and SNAP-25 through its C-terminal SNARE motif and competes with synaptobrevin-2/VAMP2 for the SNARE-complex assembly. Furthermore, amisyn contains an N-terminal pleckstrin homology domain that mediates its transient association with the plasma membrane of neurosecretory cells by binding to phospholipid PI(4,5)P2. However, unlike synaptrobrevin-2, the SNARE motif of amisyn is not sufficient to account for the role of amisyn in exocytosis: Both the pleckstrin homology domain and the SNARE motif are needed for its inhibitory function. Mechanistically, amisyn interferes with the priming of secretory vesicles and the sizes of releasable vesicle pools, but not vesicle fusion properties. Our biochemical and functional analyses of this vertebrate-specific protein unveil key aspects of negative regulation of exocytosis.
Effects of Leaf Position and Water Management on Translocation and Distribution of 14C-assimilates in Fruiting Muskmelon

Studies were carried out to elucidate the effects of leaf position and water management on the translocation and distribution of 14C-assimilates in muskmelon. 1. The fruit grew continuously until harvest and served as the main sink to the leaf which subtended the lateral fruit shoot. 2. At 43 days after anthesis all leaves exported photosynthetic assimilates mainly to the fruit. However, the contribution of each leaf to the accumulation of photosynthetic assimilates by the fruit and other sinks differed a little. It was assumed that the gradient between leaf and developing fruit changed according to the distance and the relative position between them. 3. The percentages of export of photosynthetic assimilates changed by different water management. However, it is probably not the direct effect of water management but due to the difference in the fruit ripening stage which was enhanced by water stress. Moreover, the percentage of distribution of photosynthetic assimilates to fruit was not changed significantly by different water management at the experimental stages. Consequently, the water stress caused the decrease in the photosynthetic rate and the shortened the fruit development period by hastening ripening process. Therefore, the water stress may not contribute toward increasing the amount of sugars translocated in the fruit.
Differential 3H-Thymidine Labeling of Nuclei in the Shoot Apical Meristem of Nicotiana

The apical meristem of a hybrid Nicotiana tabacum x N. rustica backcrossed to N. rustica for seven generations is very large, being 0.6 mm in diameter, and responds well to in vitro culture and surgical manipulation. Within the meristem a distal region can be distinguished from a peripheral region on the basis of intensity of cytoplasmic staining. Excised intact meristems grown on culture medium that contained 3H-thymidine incorporated the label into nuclei of the peripheral region but not into nuclei of the distal region. Experiments demonstrated that lack of labeling of distal cell nuclei was not caused by impermeability of these cells to the thymidine molecule or by damage from tritium β-emission. Lack of labeling was therefore considered to indicate lack of DNA synthesis in these nuclei during the course of an experiment. Nuclei of distal cells could be labeled with 3H-thymidine in meristems that were regenerating after surgical wounding, and a group of distal labeled cells was shown to persist in shoots subsequently grown on isotope-free culture medium for 16 days, indicating that these cells divide slowly if at all during prolonged periods of vegetative growth.