Miracle-dz/UniFAR-FTUs huggingface dataset:
A Common Insecticide Induced-Oxidative Stress in Wistar Rats: Significance for Humans and Implications for Nutritional Modulation of Insecticide Toxicity

Abstract Objective This study examined the levels of selected micronutrients and associated biochemical changes in rats exposed to Baygon® insecticide. Arsenic is a toxic metalloid commonly used in insecticides manufacture but unheralded. Methods Fifteen rats, divided into three equal groups: Group I (control); group II (administered 2.5 mg/kg sodium arsenite (SA) on alternate days for four weeks); group III (exposed to 14.0 mL Baygon® m−3 cage volume daily for four weeks). Serum levels of arsenic (As), selenium (Se) and zinc (Zn) were determined using flame atomic absorption spectrophotometry (FAAS). Reduced glutathione (GSH), glutathione peroxidase (GPx), and total protein (TP) were determined spectrophotometrically. Results Arsenic and Se levels were significantly raised in groups II and III compared with control (p < 0.05), unlike Zn levels that were significantly decreased in groups II and III (p < 0.05) in both. No significant change in the activity of GPx; though the activity increased in the group treated with SA, but decreased in the group treated with Baygon® compared to control (P < 0.05). Histology of the liver and lung was unaltered in control, but in contrast, the SA-treated group demonstrated moderate fibrous hyperplasia with prominent highly infiltrated portal area in the liver; while the lung revealed thickened alveolar walls from proliferated pneumocytes. In the Baygon®-treated group, there was mild hyperplasia of the fibrous connective tissue and congested prominent portal areas; while the lung exhibited severe thickened alveolar walls due to proliferated pneumocytes. Conclusion Exposure of rats to Baygon® elicited alteration of key trace elements involved in the antioxidant system, culminating in oxidative stress with attendant deleterious effects. One significance of this for humans is that it has great potentials for possible nutritional modulation of insecticide toxicity with micronutrients, especially with zinc, holding great promise in tropical developing countries.
Bethesda System Categories of Fine Needle Aspiration Cytology Are Not Associated with Thyroid Nodule Location: A Single Institution Experience

Introduction: Thyroid isthmus is defined as the thin band connecting thyroid tissue between both lateral thyroid lobes. Recently, a possible association between thyroid nodules located in the isthmus and malignancy was proposed. The aim of this study was to compare the frequency of The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) categories between nodules from the isthmus and nodules from both thyroid lobes. Methods: All fine needle aspiration cytology procedures performed between January 2016 and December 2019 at the Pathology Institute of Araçatuba, São Paulo, Brazil, were analyzed. For each nodule, at least 2 conventional slides were produced (1 stained by Giemsa and the other by hematoxylin and eosin). All cases were reported according to the TBSRTC. Clinical information (gender, age, and localization) and ultrasound data (size of nodules) were collected from medical requisition forms. To assess the association between the frequency of TBSRTC categories and nodule location, univariate analysis was performed using the χ2 test or Fisher’s exact test, as appropriate. A p value <0.05 was considered statistically significant. Nodules located in transition between the isthmus and a right or left lobe were included in the isthmus group. Results: Considering the p value between the TBSRTC categories and thyroid nodule location, statistic association was not observed: nondiagnostic or unsatisfactory (p = 0.1442), atypia of undetermined significance or follicular lesion of undetermined significance (p = 0.3296), follicular neoplasm or suspicious for a follicular neoplasm (p = 0.0817), suspicious for malignancy (p = 0.8464), and malignant (p = 0.1082). Conclusion: In the studied population, nodules located in the isthmus were not related to any Bethesda System category.
Cost-effectiveness of a new autoantibody test added to Computed Tomography (CT) compared to CT surveillance alone in the diagnosis of lung cancer amongst patients with indeterminate pulmonary nodules

Oncimmune's EarlyCDT®-Lung is a simple ELISA blood test that measures seven lung cancer specific autoantibodies and is used in the assessment of malignancy risk in patients with indeterminate pulmonary nodules (IPNs). The objective of this study was to examine the cost-effectiveness of EarlyCDT-Lung in the diagnosis of lung cancer amongst patients with IPNs in addition to CT surveillance, compared to CT surveillance alone which is the current recommendation by the British Thoracic Society guidelines. A model consisting of a combination of a decision tree and Markov model was developed using the outcome measure of the quality adjusted life year (QALY). A life-time time horizon was adopted. The model was parameterized using a range of secondary sources. At £70 per test, EarlyCDT-Lung and CT surveillance was found to be cost-effective compared to CT surveillance alone with an incremental cost-effectiveness ratio (ICER) of less than £2,500 depending on the test accuracy parameters used. It was also found that EarlyCDT-Lung can be priced up to £1,177 and still be cost-effective based on cost-effectiveness acceptance threshold of £20,000 / QALY. Further research to resolve parameter uncertainty, was not found to be of value. The results here demonstrate that at £70 per test the EarlyCDT-Lung will have a positive impact on patient outcomes and coupled with CT surveillance is a cost-effective approach to the management of patients with IPNs. The conclusions drawn from this analysis are robust to realistic variation in the parameters used in the model.
Live In Vivo Imaging of Plasmodium Invasion of the Mosquito Midgut

Malaria is one of the most devastating parasitic diseases in humans and is transmitted by anopheline mosquitoes. The mosquito midgut is a critical barrier that Plasmodium parasites must overcome to complete their developmental cycle and be transmitted to a new host. Here, we developed a new strategy to visualize Plasmodium ookinetes as they traverse the mosquito midgut and to follow the response of damaged epithelial cells by imaging live mosquitoes. Understanding the spatial and temporal aspects of these interactions is critical when developing novel strategies to disrupt disease transmission. ABSTRACT The mosquito midgut is a critical barrier that Plasmodium parasites must overcome to complete their developmental cycle and be transmitted to a new vertebrate host. Previous confocal studies with fixed infected midguts showed that ookinetes traverse midgut epithelial cells and cause irreversible tissue damage. Here, we investigated the spatiotemporal dynamics of ookinete midgut traversal and the response of midgut cells to invasion. A novel mounting strategy was established, suitable fluorescent dye combinations were identified and protocols optimized to label mosquito tissues in vivo, and live imaging protocols using confocal microscopy were developed. Tracking data showed that ookinetes gliding on the midgut surface travel faster and farther than those that remain in the lumen or those that have invaded the epithelium. Image analysis confirmed that parasite invasion and cell traversal occur within a couple of minutes, while caspase activity in damaged cells, indicative of cellular apoptosis, and F-actin cytoskeletal rearrangements in cells extruded into the gut lumen persist for several hours. This temporal difference highlights the importance of hemocyte-mediated cellular immunity and the mosquito complement system to mount a coordinated and effective antiplasmodial response. This novel in vivo imaging protocol allowed us to continuously observe individual ookinetes in live mosquitoes within the gut lumen and during cell traversal and to capture the subsequent cellular responses to invasion in real time for several hours, without loss of tissue integrity. IMPORTANCE Malaria is one of the most devastating parasitic diseases in humans and is transmitted by anopheline mosquitoes. The mosquito midgut is a critical barrier that Plasmodium parasites must overcome to complete their developmental cycle and be transmitted to a new host. Here, we developed a new strategy to visualize Plasmodium ookinetes as they traverse the mosquito midgut and to follow the response of damaged epithelial cells by imaging live mosquitoes. Understanding the spatial and temporal aspects of these interactions is critical when developing novel strategies to disrupt disease transmission.
NGAL Correlates with Femoral and Carotid Plaque Volume Assessed by Sonographic 3D Plaque Volumetry

Background/Objectives: Inflammation represents a cornerstone in the development of atherosclerosis and early detection is essential to avoid cardiovascular events. Biomarkers like interleukin-1 beta, interleukin-6, or high sensitivity CRP (hs-CRP) have been investigated intensively in this field. Since they have several limitations, additional biomarkers are needed for cardiovascular risk stratification. The acute phase protein, neutrophil gelatinase-associated lipocalin (NGAL), modulates inflammation and is elevated in cardiovascular disease (CVD). Moreover, it contributes to plaque destabilization. Methods: In this prospective, single-center study, we included 323 asymptomatic patients with at least one cardiovascular risk factor or established CVD. NGAL levels were measured in plasma samples using a commercially available ELISA. Carotid, femoral, and total atherosclerotic plaque volumes (PV) were measured using a 3D ultrasound system (Philips iU22). Patients were separated into a low (n = 243) and high (n = 80) total PV group. Results: NGAL was significantly higher in patients with high total PV versus patients with low total PV. The NGAL amplitude for the prediction of high total PV was significantly higher when compared with hs-CRP. A high predictive value could also be observed for patients without established CVD. Conclusion: NGAL seems to be a promising biomarker for the identification of asymptomatic patients with atherosclerotic disease.
Paired tumor sequencing and germline testing in breast cancer management: An experience of a single academic center

Genetic testing for cancer predisposition is recommended to women with breast cancer who meet the criteria for such testing. After the FDA approvals of the poly ADP ribose polymerase (PARP) inhibitors, olaparib and talazoparib, for treatment of metastatic breast cancer, carrying germline mutations in BRCA1 and BRCA2 genes, the genetic testing result has become critical in their care. With the recent FDA approval of alpelisib for the treatment of PIK3CA‐mutated hormone‐receptor positive metastatic breast cancer, tumor molecular profiling to identify somatic mutations and potential molecularly targeted agents is increasingly utilized in the treatment of advanced breast cancer.
Branched-chain ketoacid overload inhibits insulin action in the muscle

Branched-chain α-keto acids (BCKAs) are catabolites of branched-chain amino acids (BCAAs). Intracellular BCKAs are cleared by branched-chain ketoacid dehydrogenase (BCKDH), which is sensitive to inhibitory phosphorylation by BCKD kinase (BCKDK). Accumulation of BCKAs is an indicator of defective BCAA catabolism and has been correlated with glucose intolerance and cardiac dysfunction. However, it is unclear whether BCKAs directly alter insulin signaling and function in the skeletal and cardiac muscle cell. Furthermore, the role of excess fatty acids (FAs) in perturbing BCAA catabolism and BCKA availability merits investigation. By using immunoblotting and ultra-performance liquid chromatography MS/MS to analyze the hearts of fasted mice, we observed decreased BCAA-catabolizing enzyme expression and increased circulating BCKAs, but not BCAAs. In mice subjected to diet-induced obesity (DIO), we observed similar increases in circulating BCKAs with concomitant changes in BCAA-catabolizing enzyme expression only in the skeletal muscle. Effects of DIO were recapitulated by simulating lipotoxicity in skeletal muscle cells treated with saturated FA, palmitate. Exposure of muscle cells to high concentrations of BCKAs resulted in inhibition of insulin-induced AKT phosphorylation, decreased glucose uptake, and mitochondrial oxygen consumption. Altering intracellular clearance of BCKAs by genetic modulation of BCKDK and BCKDHA expression showed similar effects on AKT phosphorylation. BCKAs increased protein translation and mTORC1 activation. Pretreating cells with mTORC1 inhibitor rapamycin restored BCKA's effect on insulin-induced AKT phosphorylation. This study provides evidence for FA-mediated regulation of BCAA-catabolizing enzymes and BCKA content and highlights the biological role of BCKAs in regulating muscle insulin signaling and function.
The Effect of Taxonomic Corrections on Phanerozoic Generic Richness Trends in Marine Bivalves with a Discussion on the Clade's Overall History

Abstract. This study uses a comprehensive, revised, and updated global bivalve dataset combining information from two major databases available to study temporal trends in Phanerozoic bivalve richness: the Sepkoski Compendium and the Paleobiology Database. This compilation results in greater taxonomic and stratigraphic coverage than possible with either of the two databases alone. However, there are challenges in directly comparing these two sources due to differences in their taxonomic designations and stratigraphic range information. Moreover, both of these datasets are fraught with a number of taxonomic errors, which can significantly bias the overall richness estimate. Additionally, a substantial number of taxonomic corrections were made before a new Phanerozoic bivalve richness curve was produced. The new generic taxonomic curve is comparablewith the trajectory of the Sepkoski'smodern fauna and shows rapid and substantial diversification through the Ordovician, followed by a Paleozoic plateau, aMesozoic high, and Cenozoic diversification after a small reduction in richness associated with the K/Pg extinction. The steepCenozoic rise documented in the rawrichness curve derived fromthe new dataset is likely real, and reflects the overall robustness and completeness of the bivalve fossil record.
Therapeutic Targeting of Mitochondrial One-Carbon Metabolism in Cancer

One-carbon (1C) metabolism encompasses folate-mediated 1C transfer reactions and related processes, including nucleotide and amino acid biosynthesis, antioxidant regeneration, and epigenetic regulation. 1C pathways are compartmentalized in the cytosol, mitochondria, and nucleus. 1C metabolism in the cytosol has been an important therapeutic target for cancer since the inception of modern chemotherapy, and “antifolates” targeting cytosolic 1C pathways continue to be a mainstay of the chemotherapy armamentarium for cancer. Recent insights into the complexities of 1C metabolism in cancer cells, including the critical role of the mitochondrial 1C pathway as a source of 1C units, glycine, reducing equivalents, and ATP, have spurred the discovery of novel compounds that target these reactions, with particular focus on 5,10-methylene tetrahydrofolate dehydrogenase 2 and serine hydroxymethyltransferase 2. In this review, we discuss key aspects of 1C metabolism, with emphasis on the importance of mitochondrial 1C metabolism to metabolic homeostasis, its relationship with the oncogenic phenotype, and its therapeutic potential for cancer.
Estimating Throwing Speed in Handball Using a Wearable Device

Throwing speed is likely a key determinant of shoulder-specific load. However, it is difficult to estimate the speed of throws in handball in field-based settings with many players due to limitations in current technology. Therefore, the purpose of this study was to develop a novel method to estimate throwing speed in handball using a low-cost accelerometer-based device. Nineteen experienced handball players each performed 25 throws of varying types while we measured the acceleration of the wrist using the accelerometer and the throwing speed using 3D motion capture. Using cross-validation, we developed four prediction models using combinations of the logarithm of the peak total acceleration, sex and throwing type as the predictor and the throwing speed as the outcome. We found that all models were well-calibrated (mean calibration of all models: 0.0 m/s, calibration slope of all models: 1.00) and precise (R2 = 0.71–0.86, mean absolute error = 1.30–1.82 m/s). We conclude that the developed method provides practitioners and researchers with a feasible and cheap method to estimate throwing speed in handball from segments of wrist acceleration signals containing only a single throw.
Educational Interventions for Teaching Evidence-Based Practice to Undergraduate Nursing Students: A Scoping Review

Background: Evidence-based practice (EBP) is the appropriate approach to guide healthcare personnel in their clinical practice. Despite the importance of EBP, undergraduate nursing students are not very much engaged and have a lack of knowledge and skills. Aim: The aim of this study was to gather, assess and synthesize evidence on educational interventions promoting evidence-based practice competencies in traditional undergraduate nursing students. Methods: This is a scoping review on sixteen English and non-English databases. A data extraction form was established including authors, year of publication, country, types of participant, specific objectives, study design, educational intervention, comparison if existed, and outcomes of significance. Results: The search strategy retrieved 8901 records in total. After screening for duplicates and eligibility, 20 articles were included in the qualitative synthesis. Improvement in EBP domains such as knowledge, skills, attitudes/behaviours, EBP beliefs, use, practice, level of evidence, critical thinking and future use of EBP were mentioned and assessed in different studies. Conclusions: EBP training can improve nursing students’ capacity in healthcare provision. Teaching EBP competencies along undergraduate nursing curricula should be a high priority at nursing programmes. The use of innovative approaches seems to be more effective than traditional ways. Education of EBP increases its future use and critical thinking and EBP programs improve self-efficacy and the level of evidence utilization.
Agricultural Greenhouses Detection in High-Resolution Satellite Images Based on Convolutional Neural Networks: Comparison of Faster R-CNN, YOLO v3 and SSD

Agricultural greenhouses (AGs) are an important facility for the development of modern agriculture. Accurately and effectively detecting AGs is a necessity for the strategic planning of modern agriculture. With the advent of deep learning algorithms, various convolutional neural network (CNN)-based models have been proposed for object detection with high spatial resolution images. In this paper, we conducted a comparative assessment of the three well-established CNN-based models, which are Faster R-CNN, You Look Only Once-v3 (YOLO v3), and Single Shot Multi-Box Detector (SSD) for detecting AGs. The transfer learning and fine-tuning approaches were implemented to train models. Accuracy and efficiency evaluation results show that YOLO v3 achieved the best performance according to the average precision (mAP), frames per second (FPS) metrics and visual inspection. The SSD demonstrated an advantage in detection speed with an FPS twice higher than Faster R-CNN, although their mAP is close on the test set. The trained models were also applied to two independent test sets, which proved that these models have a certain transability and the higher resolution images are significant for accuracy improvement. Our study suggests YOLO v3 with superiorities in both accuracy and computational efficiency can be applied to detect AGs using high-resolution satellite images operationally.
Status of Land Birds on Selected Islands in the Ha'apai Group, Kingdom of Tonga

Based on fieldwork in 1995 and 1996, I assess the distribution, relative abundance, and habitat requirements of indigenous species of land birds on 13 islands in the Ha'apai Group, Kingdom of Tonga. Among the islands visited, primary forest still exists only on the large (46.6 km 2), high (558 m) volcanic island of Tofua. Vegetation on the 12 smaller (0.15-13.3 km 2), lower (6-45 m) islands is dominated by a mosaic of active and abandoned agricultural plots, nearly all with an over story of coconut trees. Because of cultivation practices, very little of this vegetation is reverting to secondary forest. Of the 15 resident species of land birds that survive on these islands, nine are widespread and at least locally common within Ha'apai, although only four (Gallirallus philippensis, Ptilinopus porphyraceus, Halcyon chloris, Aplonis tabuensis) certainly or probably occur nowadays on all 13 islands. Three species (Gallicolumba stairii, Ptilinopus perousii, Clytorhynchus vitiensis) are extirpated or extremely rare on all islands surveyed except Tofua. Overall species richness and abundance of land birds are much greater on Tofua than on the other islands. This difference may be due more to the presence of primary forest on Tofua than to Tofua's greater area and elevation. This PAPER DESCRIBES the current status of native land birds on 13 islands in the Ha'apai Group, Kingdom of Tonga. The field research was conducted to fulfill two goals: first, to describe long-term (prehistoric versus modem) changes in the distribution of each species; and Originally published in Pacific Science (1998), vol. 52, no. 1: 14-34 © Text and images reproduced by kind permission of David W. Steadman secondly, to assess the relative abundance and habitat relationships of each species to provide information for conservation programs. The first goal includes studying bird bones from archaeological sites and is the focus of another publication. Here I emphasize the second goal. Tonga is part of the West Polynesian avifaunal region that comprises Niue, Samoa, Wallis and Futuna, Fiji, and Rotuma (Watling 1982, Steadman 1993, 1997b). The extant, indigenous land birds of Tonga represent 17 genera (none endemic) and 18 species (two endemic). The birds of Ha'apai are not well known. They are treated in a general way in regional books, such as duPont (1976), Watling (1982), and Pratt et al. (1987). In spite of accounts of selected species on certain islands (Gill 1988, Rinke et al. 1992), meaningful lists of species (sensu Remsen 1994) were not available for any individual island in Ha'apai before this study, nor had relative abundances been estimated.
Standing stock and production of phytoplankton in the estuary of the Chang-jiang (Yangste River) and the adjacent East China Sea

We determined cell density and dominant species of eukaryotic phytoplankton, chlorophyll concentration and primary production in the Changjiang estuary and dilution zone in January and July 1986. In winter, phytoplankton was dominated by small cells (< 10 pm), closely associated to particulate matter. In summer, the most striking feature of chlorophyll and primary production distributions was the presence of sharp maxima localized about 100 km offshore at salinities between 25 and 30 960 and related to diatom populations. The critical factor responsible for these maxima was the increased light avadability following sedimentation of the particulate matter oriqnating from the river, as observed in front of other major world rivers such as the Zaire and the Amazon. This photosynthetic activity resulted in high oxygen concentrations and phosphate depletion in the surface layer. In both seasons, in situ area1 productivity and light availability were related by a simple empirical relationship similar to that established for other medium-sized estuaries.