Miracle-dz/UniFAR-FTUs huggingface dataset:
Methylene Blue and Iron (II) Adsorption onto Raphia Hookeri Seed: A Comparative Equilibrium Isotherm Study

In this study, activated carbon prepared from Raphia hookeri seed was employed as an adsorbent to take up methylene blue (an organic ion-in-solution) and iron (II), a metal ion-in-solution. The biosorbent was prepared using chemical activation using sodium hydroxide (NaOH) as an impregnating agent. Calibration was performed with a focus on the change in the adsorbate concentration. The equilibrium isotherms study was conducted by considering a range of sorbate concentrations, to determine the optimal conditions for the adsorption systems. The same sorbate volume (10 ml), and the same dosage (0.3g) of biosorbent were used for both adsorption processes. Linear analysis was used to compare the best-fitting isotherms. Langmuir, Freundlich, and Dubinin-Radushkevich models were tested. The highest coefficient of determination values (R2) for both iron (II) and Methylene blue was obtained from the Langmuir isotherm as 0.9266 and 0.9572, respectively, compared to values from the other isotherms tested – indicative of Langmuir isotherm’s superior fit. Estimated values of ‘separation factor’, KR, for Methylene blue (0.29457), however, was lower than that of iron (II) (0.42141) - an indication that the sorbent treats methylene blue better than iron (II), though both processes are feasible.
Resistance of the Wheat Cultivar ‘Renan’ to Septoria Leaf Blotch Explained by a Combination of Strain Specific and Strain Non-Specific QTL Mapped on an Ultra-Dense Genetic Map

Quantitative resistance is considered more durable than qualitative resistance as it does not involve major resistance genes that can be easily overcome by pathogen populations, but rather a combination of genes with a lower individual effect. This durability means that quantitative resistance could be an interesting tool for breeding crops that would not systematically require phytosanitary products. Quantitative resistance has yet to reveal all of its intricacies. Here, we delve into the case of the wheat/Septoria tritici blotch (STB) pathosystem. Using a population resulting from a cross between French cultivar Renan, generally resistant to STB, and Chinese Spring, a cultivar susceptible to the disease, we built an ultra-dense genetic map that carries 148,820 single nucleotide polymorphism (SNP) markers. Phenotyping the interaction was done with two different Zymoseptoria tritici strains with contrasted pathogenicities on Renan. A linkage analysis led to the detection of three quantitative trait loci (QTL) related to resistance in Renan. These QTL, on chromosomes 7B, 1D, and 5D, present with an interesting diversity as that on 7B was detected with both fungal strains, while those on 1D and 5D were strain-specific. The resistance on 7B was located in the region of Stb8 and the resistance on 1D colocalized with Stb19. However, the resistance on 5D was new, so further designated Stb20q. Several wall-associated kinases (WAK), nucleotide-binding and leucine-rich repeats (NB-LRR) type, and kinase domain carrying genes were present in the QTL regions, and some of them were expressed during the infection. These results advocate for a role of Stb genes in quantitative resistance and for resistance in the wheat/STB pathosystem being as a whole quantitative and polygenic.
A survey study of adults with chronic conditions: Examining the correlation between patient activation and health locus of control

Objectives This study aimed to examine (1) the association between patient activation (PA), health locus of control (HLOC), sociodemographic and clinical factors, and (2) the effect of HLOC dimensions, sociodemographic and clinical factors on PA. Methods Three hundred U.S. adults, with at least one chronic condition (CC) were recruited through Amazon Mechanical Turk and completed an online survey which included sociodemographic questions, the Patient Activation Measure® - 10, and the Multidimensional Locus of Control (MHLC) - Form B. Statistical analyses, including descriptive, correlation, and multiple linear regression, were conducted using IBM SPSS v25. Results Of the 300 participants, more than half were male (66.3%), White (70.7%), with at least a college degree (76.0%), and employed full-time (79.0%). The average PA score was 68.8 ± 14.5. Multiple linear regression indicated that participants who reported they were Black, retired, with a greater number of CCs, and with higher scores in Chance MHLC had higher PA, while participants with higher scores in Internal MHLC, were unemployed and reported to have been affected by COVID-19-related worry or fear to manage their CC, had lower PA. Discussion HLOC dimensions should be addressed concurrently with PA for patients with CCs, thus adding to a more patient-centered clinical approach.
Effect of Combined Grape Seed Extract and L-Citrulline Supplementation on Hemodynamic Responses to Exercise in Young Males

Abstract Grape seed extract (GSE) or L-citrulline supplement has been known to increase nitric oxide (NO) bioavailability and enhance endothelial-mediated vasodilation. Accordingly, to examine the additive benefits of combination of the two supplementations on hemodynamic responses to dynamic exercise, young, healthy males were recruited for this study. Effects of 7 days of 1) GSE + L-citrulline, 2) GSE, 3) L-citrulline, and 4) placebo supplementation on systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial blood pressure (MAP), cardiac output, total vascular conductance (TVC), and oxygen (O2) consumption were examined at rest and during cycling exercise. Compared with placebo, GSE, L-citrulline, and combined supplementations did not reduce SBP, DBP, and MAP, while cardiac output (placebo; 23.6 ± 1.3 L/min, GSE; 25.7 ± 1.1 L/min; L-citrulline, 25.2 ± 1.2 L/min; GSE + L-citrulline; 25.3 ± 0.9 L/min) and TVC (placebo; 234.7 ± 11.3 ml/min/mmHg, GSE; 258.3 ± 10.6 ml/min/mmHg; L-citrulline, 255.2 ± 10.6 ml/min/mmHg; GSE + L-citrulline; 260.4 ± 8.9 ml/min/mmHg) were increased at only the 80% workload (p < 0.05). Compared with placebo and L-citrulline, GSE and combined supplementations had a reduction in VO2 across workloads (p < 0.05). However, there was no additive benefits on these variables. We conclude that supplementation with GSE, L-citrulline, and combined supplementations increased cardiac output due partially to decreased vascular resistance. Our findings suggest that GSE may act as an ergogenic aid that can improve O2 delivery to exercising muscles.
Hemato-vascular specification requires arnt1 and arnt2 genes in zebrafish embryos

During embryonic development, a subset of cells in the mesoderm germ layer are specified as hemato- vascular progenitor cells, which then differentiate into endothelial cells and hematopoietic stem and progenitor cells. In zebrafish, the transcription factor npas4l, also known as cloche, is required for the specification of hemato-vascular progenitor cells. However, it is unclear if npas4l is the sole factor at the top of the hemato-vascular specification cascade. Here we show that arnt1 and arnt2 genes are required for hemato-vascular specification. We found that arnt1;arnt2 double homozygous mutant zebrafish embryos (herein called arnt1/2 mutants), but not arnt1 or arnt2 single mutants, lack blood cells and most vascular endothelial cells. arnt1/2 mutants have reduced or absent expression of etsrp and tal1, the earliest known endothelial and hematopoietic transcription factor genes. npas4l and the arnt genes are PAS domain-containing bHLH transcription factors that function as dimers. We found that Npas4l binds both Arnt1 and Arnt2 proteins in vitro, consistent with the idea that PAS domain- containing bHLH transcription factors act in a multimeric complex to regulate gene expression. Our results demonstrate that npas4l, arnt1 and arnt2 act together to regulate endothelial and hematopoietic cell fate, where each gene is necessary, but by itself not sufficient, to drive hemato-vascular specification. Our results also demonstrate that arnt1 and arnt2 act redundantly in a transcriptional complex containing npas4l, but do not act redundantly when interacting with another PAS domain- containing bHLH transcription factor, the aryl hydrocarbon receptor. Altogether, our data enhance our understanding of hemato-vascular specification and the function of PAS domain-containing bHLH transcription factors.
Deciphering in vitro mutated sugarcane clones for growth parameters

Callus derived from sugarcane variety CoN – 13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 - 30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, Maximum shoot length (26.6 cm) was observed in the treatment, EMS 0.2% + 30 min. Maximum root length (12.8 cm) was observed in treatment MMS 0.3% + 30 min. A higher concentration of all mutagenic agents registered higher root length with the maximum of 1.14 g was observed in treatment EMS 0.3% + 60 min. Maximum leaf area (44.7 cm 2 /plant) was observed in treatment MMS 0.2% + 30 min. Irrespective to concentration SA and EMS found significant for this character. Maximum chlorophyll content index (4.87) was observed in treatment EMS 0.2% + 30 min. In case of this character higher to lower concentration as well as time period influenced in both higher and lower directions. Among the treatments, the maximum multiplication rate (12.45%) was observed in treatment EMS 0.2% + 30 min. Both factors showed different effects on this character. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decreases with the increase in the time interval.
Correlation Analysis of Protein Expression of 10 HDAC/Sirtuin Isoenzymes with Sensitivities of 23 Anticancer Drugs in 17 Cancer Cell Lines and Potentiation of Drug Activity by Co-Treatment with HDAC Inhibitors

Simple Summary Protein expression profiles of 10 HDAC/Sirtuin isoenzymes in two panels of human cancer cell lines were compared with each other and with the potencies of various anticancer drugs by Pearson and Spearman correlation analysis to identify patterns of enzyme expression and anticancer activity. Furthermore, the NCI COMPARE database was used to identify possible correlations between the mRNA expression in a 60 cancer cell panel and the potency of the same anticancer drugs. While several interesting correlations were found within both data sets, none of these correlations were identical in the two sets of data, suggesting that protein and mRNA expression profiles are not comparable. Combination treatments with several HDAC inhibitors with a number of the anticancer drugs revealed interesting synergistic effects that were in keeping with some of the correlations predicted by our protein expression analysis. Abstract Inhibiting the activity of histone deacetylase (HDAC) is an ongoing strategy in anticancer therapy. However, to our knowledge, the relationships between the expression of HDAC proteins and the antitumor drug sensitivity of cancer cells have not been studied until now. In the current work, we investigated the relative expression profiles of 10 HDAC isoenzymes comprising the classes I–III (HDAC1/2/4/6; Sirt1/2/3/5/6/7) in a panel of 17 cancer cell lines, including the breast, cervix, oesophageal, lung, oral squamous, pancreas, as well as urinary bladder carcinoma cells. Correlations between the data of mRNA expression for these enzymes obtained from the National Cancer Institute (NCI) 60 cancer cell line program were also examined. Next, we performed univariate analysis between the expression patterns of HDAC/Sirt isoenzymes with the sensitivity of a 16 cell panel of cancer cell lines towards several antitumor drugs. In a univariate correlation analysis, we found a strong relation between Sirt2 expression and cytotoxicity caused by busulfan, etoposide, and hydroxyurea. Moreover, it was identified that Sirt5 correlates with the effects exerted by oxaliplatin or topotecan, as well as between HDAC4 expression and these two drugs. Correlations between the data of mRNA expression for enzymes with the potencies of the same anticancer agents obtained from the NCI 60 cancer cell line program were also found, but none were the same as those we found with our protein expression data. Additionally, we report here the effects upon combination of the approved HDAC inhibitor vorinostat and one other known inhibitor trichostatin A as well as newer hetero-stilbene and diazeno based sirtuin inhibitors on the potency of cisplatin, lomustine, and topotecan. For these three anticancer drugs, we found a significantly enhanced cytotoxicity when co-incubated with HDAC inhibitors, demonstrating a potentially beneficial influence of HDAC inhibition on anticancer drug treatment.
Bispectral Index Monitoring and Observer Rating Scale Correlate with Dreaming during Propofol Anesthesia for Gastrointestinal Endoscopies

Background and objectives: Dreaming is a commonly reported side effect of propofol anesthesia. Materials and Methods: We investigated the inci-dence and character of dreams in patients undergoing intravenous propofol anesthesia and cor-related it with an observer rating scale of facial expression on the seven-point scale from pain to smile. A total of 124 patients undergoing gastrointestinal endoscopy were recruited in the pro-spective observational study. Bispectral index (BIS), blood pressure (BP), and pulse were moni-tored. Upon emergence from anesthesia, the patient’s facial expression was rated numerically. Thereafter, patients were asked whether they had dreams and to rate their dreams as pleasant or unpleasant. The mean age of participants was 53; body mass index, 26.17; duration of procedure, 20 min; and average propofol dose, 265 mg. Results: Dreaming was reported by 43% of patients. Dreams were pleasant in all but one patient. There was a significant correlation of the observer’s rating of facial expression with dreaming (r = 0.260; p = 0.004). Dreamers had higher scores of observer rating of facial expression (1 (0–2) vs. 0.5 (0–1), p = 0.006). Conclusions: BIS values were lower in the dreamers vs. non-dreamers 2 min after the endoscopy started (48 (43–62) vs. 59 (45–71), p = 0.038). Both BIS and observer ratings correlate with dreaming in patients undergoing gastrointestinal endos-copy. Trial registration number: NCT04235894.
Environmental Determinates of Distribution for Dragonfly Nymphs (Odonata: Anisoptera) in Urban and Non-Urban East Texas Streams, USA

We collected environmental and habitat data for nymphs of 12 dragonfly species (Odonata: Anisoptera) from 91 stream sites throughout eastern Texas, including urban and non-urban locations. Understanding the relationship of dragonflies to habitat structure and other environmental variables is crucial for the purpose of conserving these insects and better using them as predictive tools for water quality assessments, and refining tolerance values. The objectives of this study were to determine the key environmental variables influencing the diversity and distribution of dragonflies in eastern Texas streams, and further determine if differences in those factors could be observed between urban and nonurban sites. We collected samples separately from benthic habitats and woody snag habitats. Significantly fewer sites were observed to have dragonfly species on snag habitat (mean = 1.25) compared to benthic samples (mean = 14.67) (t-test, p = 0.001). The number of dragonfly species collected among non-urban streams (mean = 9.83) was not significantly different than urban streams (mean = 6.08; t-test, p = 0.07). Detrended correspondence analysis of benthic and snag habitat data collected from non-urban and urban locations showed that most of the species are oriented most closely to benthic habitats in non-urban streams. Snag habitat was shown to be poorly ordinated for all of the species. A canonical correspondence analysis of 29 water quality and habitat variables as environmental determinants of dragonfly diversity and distribution showed that distributional relationships among species are complex and often described by multiple environmental factors.
Understanding the Relationship between Fetal Alcohol Spectrum Disorder (FASD) and Criminal Justice: A Systematic Review

Prenatal alcohol exposure is considered one of the main causes of preventable birth disorders; however, it represents the main form of developmental delay in the world. Among the so-called secondary disabilities related to fetal alcohol spectrum disorder (FASD), there is a close connection with criminal behavior. This systematic review aims to provide up-to-date information about the relationship between FASD subjects and criminal justice analyzing different aspects. In light of the results of this review, a further goal is to provide several suggestions in order to reduce the public cost impact of FASD. A systematic review of the literature was conducted according to the PRISMA guidelines, producing 20 articles that met the inclusion criteria. Based on the results published in the selected studies, fetal alcohol syndrome (FAS) is a leading cause of preventable birth disorders and developmental disabilities in newborns. Moreover, these subjects seem to be more inclined to criminal acts compared to others. In conclusion, it should be pointed out that FASD entails high public health costs, both regarding the support measures provided to the affected individual and to their family, as well as the cost and social impact of any criminal offenses committed.
Integration of renewable energies in the electricity distribution system

Many countries have set up mechanism support in favor of renewable energy development at a short or long term, while, in parallel, long-term scenarios of predominantly renewable energy provisioning are studied. Some of these energies are intermittent and non-dispatchable and, due to the low economies of scale they exhibit, can be deployed in a decentralized manner, integrated in buildings and connected to the distribution system.However, the methods and tools currently used to plan and operate the power system rely on hypothesis of centralized generation and passive distribution network. In this context, this thesis contributes to the development of a method aimed at simulated an active distribution network by proposing three novel approaches: a decomposition and coordination method allowing the integrated simulation of the centralized power system and the active distribution network, an algorithm to solve the single-stage optimal power flow in the distribution system as well as two criticality criteria allowing to decrease the computational burden of the simulation when it is used for planning purposes.
Maximally Permissive Deadlock and Livelock Avoidance for Automated Manufacturing Systems via Critical Distance

The problem under consideration in this paper is how to avoid deadlocks and livelocks in the paradigm of Petri nets (PNs). Although deadlock and livelock avoidance has been extensively studied in existing literature, fewer results can be applied to practical automated manufacturing systems (AMSs) due to expensive computations and reduced permissiveness. In this paper, we propose an efficient and maximally permissive control scheme to avoid deadlocks and livelocks by using critical distance. From the perspective of the reachability graph of a PN, critical distance is equal to the maximum number of transitions among all transition sequences from any critical state to its corresponding deadlock or livelock state. First, we show how to calculate the critical distance of a PN in an efficient way by using a portion of reachable states. Then, local reachability graphs are established based on critical distance to avoid deadlocks and livelocks. The provided policy is maximally permissive since only the necessary minimum number of illegal transitions are forbidden at each state. Several representative examples are presented to illustrate our approach. Note to Practitioners—Deadlock and livelock avoidance is a crucial problem in automated manufacturing systems (AMSs). Various methods have been proposed to deal with deadlocks and livelocks by researchers and practitioners. However, there exist fewer works that focus on deep exploration regarding how long a deadlock or livelock will occur from a safe state. As a consequence, the existing works suffer from the disadvantages of being too aggressive or conservative, i.e., checking the whole state space or forbidding many acceptable states when avoiding deadlocks and livelocks. Through the analysis of a partial of reachable states, this paper derives the critical distance of Petri nets modeling AMSs, which is equal to the maximum number of transitions among all transition sequences from any critical marking to its corresponding deadlock or livelock marking. All deadlocks and livelocks, if exist, can be detected at any given marking within the critical distance. Our approach not only is maximally permissive but also explores only a partial of states, thereby mitigating state explosion problem.
Macrophage and Neutrophil Interactions in the Pancreatic Tumor Microenvironment Drive the Pathogenesis of Pancreatic Cancer

Simple Summary The survival rates for patients with pancreatic adenocarcinoma are very low. This dismal prognosis is due in part to late detection and early development of metastases, and successful treatments for pancreatic adenocarcinoma are also lacking. One potential method of treatment is immunotherapy, which has been successfully implemented in several cancers. Despite success in other cancer types, there has been little progress in pancreatic adenocarcinoma. To understand these shortcomings, we explore the roles of macrophages and neutrophils, two prominent immune cell types in the pancreatic tumor environment. In this review, we discuss how macrophages and neutrophils lead to the harsh environment that is unique to pancreatic adenocarcinoma. We further explore how these immune cells can impact standard of care therapies and decrease their effectiveness. Macrophages and neutrophils could ultimately be targeted to improve outcomes for patients with pancreatic adenocarcinoma. Abstract Despite modest improvements in survival in recent years, pancreatic adenocarcinoma remains a deadly disease with a 5-year survival rate of only 9%. These poor outcomes are driven by failure of early detection, treatment resistance, and propensity for early metastatic spread. Uncovering innovative therapeutic modalities to target the resistance mechanisms that make pancreatic cancer largely incurable are urgently needed. In this review, we discuss the immune composition of pancreatic tumors, including the counterintuitive fact that there is a significant inflammatory immune infiltrate in pancreatic cancer yet anti-tumor mechanisms are subverted and immune behaviors are suppressed. Here, we emphasize how immune cell interactions generate tumor progression and treatment resistance. We narrow in on tumor macrophage (TAM) spatial arrangement, polarity/function, recruitment, and origin to introduce a concept where interactions with tumor neutrophils (TAN) perpetuate the microenvironment. The sequelae of macrophage and neutrophil activities contributes to tumor remodeling, fibrosis, hypoxia, and progression. We also discuss immune mechanisms driving resistance to standard of care modalities. Finally, we describe a cadre of treatment targets, including those intended to overcome TAM and TAN recruitment and function, to circumvent barriers presented by immune infiltration in pancreatic adenocarcinoma.