Miracle-dz/UniFAR-FTUs huggingface dataset:
Exogenous Vimentin Supplementation Transiently Affects Early Steps during HPV16 Pseudovirus Infection

Understanding and modulating the early steps in oncogenic Human Papillomavirus (HPV) infection has great cancer-preventative potential, as this virus is the etiological agent of virtually all cervical cancer cases and is associated with many other anogenital and oropharyngeal cancers. Previous work from our laboratory has identified cell-surface-expressed vimentin as a novel HPV16 pseudovirus (HPV16-PsVs)-binding molecule modulating its infectious potential. To further explore its mode of inhibiting HPV16-PsVs internalisation, we supplemented it with exogenous recombinant human vimentin and show that only the globular form of the molecule (as opposed to the filamentous form) inhibited HPV16-PsVs internalisation in vitro. Further, this inhibitory effect was only transient and not sustained over prolonged incubation times, as demonstrated in vitro and in vivo, possibly due to full-entry molecule engagement by the virions once saturation levels have been reached. The vimentin-mediated delay of HPV16-PsVs internalisation could be narrowed down to affecting multiple steps during the virus’ interaction with the host cell and was found to affect both heparan sulphate proteoglycan (HSPG) binding as well as the subsequent entry receptor complex engagement. Interestingly, decreased pseudovirus internalisation (but not infection) in the presence of vimentin was also demonstrated for oncogenic HPV types 18, 31 and 45. Together, these data demonstrate the potential of vimentin as a modulator of HPV infection which can be used as a tool to study early mechanisms in infectious internalisation. However, further refinement is needed with regard to vimentin’s stabilisation and formulation before its development as an alternative prophylactic means.
Evaluating Operator-Machine Interactions in Comparative Time Studies

Comparative experimental studies offer possibilities for reducing the uncontrolled variation in time studies of forest work. However, the factor "Operator" has often been found to be very difficult to keep constant when working with different machines. Analysis of variance often indicates that an interaction between operator and machine reduces opportunities to make generalizations. The objective of this study was to develop and test a method for analysing and correcting for operator-machine interaction effects in comparative time studies encompassing few operators. A new variable "Adaptation" was introduced to describe differences in the operators' degree of adaptation to various machines. In the present study the numeric value of the variable was estimated from (a) previous experience, (b) the subjective feeling of adaptation by the operator, and (c) a standardized time study of loading from a pile. Analysis of variance showed that differences in adaptation could explain the interaction effects in both loading and unloading. The remaining effects of the interaction between operator and machine were not significant. The method seems to offer more possibilities to generalize and statistically validate differences, even when the number of operators is limited.
Psychophysiological Regulation and Classroom Climate Influence First and Second Graders’ Well-Being: The Role of Body Mass Index

This study examines the associations between physical and emotional well-being and classroom climate, cardiac vagal response, and body mass index (BMI) in a sample of 6- to-8-year-olds. Specifically, we expected a direct link between classroom climate, vagal withdrawal, BMI and children’s physical and emotional comfort. Furthermore, we explored whether these individual and environmental characteristics influenced well-being in an interactive fashion. Participants were 142 (63 boys, 44%) first and second graders living in the North of Italy who were interviewed on their emotional and physical comfort. Heart rate and a measure of vagal influence on the heart (cardiac vagal tone) were recorded at rest and during an oral academic test. Height and weight were collected. Classroom climate was positively linked with physical well-being, whereas emotional well-being was negatively related with BMI. In addition, an inverted U-shaped effect of cardiac vagal withdrawal (i.e., cardiac vagal tone during stress minus resting vagal tone) on emotional well-being was found. Two regression models highlighted the role played by BMI when interacting with vagal withdrawal in predicting children’s physical and emotional well-being. The interplay between BMI and cardiac vagal withdrawal played an important role in primary school children’s well-being. From a clinical perspective, preventive training to improve autonomic regulation in concert with interventions promoting healthy eating attitudes might be critical for supporting primary school children’s emotional and physical health.
Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro

Mesenchymal stem cells (MSCs) have been shown to suppress tumor growth, inhibit angiogenesis, regulate cellular signaling, and induce apoptosis in cancer cells. We have earlier reported that placenta-derived decidua parietalis mesenchymal stem/stromal cells (DPMSCs) not only retained their functional characteristics in the cancer microenvironment but also exhibited increased expression of anti-apoptotic genes, demonstrating their anti-tumor properties in the tumor setting. In this study, we have further evaluated the effects of DPMSCs on the functional outcome of human breast cancer cell line MDA231. MDA231 cells were exposed to DPMSCs, and their biological functions, including adhesion, proliferation, migration, and invasion, were evaluated. In addition, genomic and proteomic modifications of the MDA231 cell line, in response to the DPMSCs, were also evaluated. MDA231 cells exhibited a significant reduction in proliferation, migration, and invasion potential after their treatment with DPMSCs. Furthermore, DPMSC treatment diminished the angiogenic potential of MDA231 cells. DPMSC treatment modulated the expression of various pro-apoptotic as well as oncogenes in MDA231 cells. The properties of DPMSCs to inhibit the invasive characteristics of MDA231 cells demonstrate that they may be a useful candidate in a stem-cell-based therapy against cancer.
Economic analysis for demand-side hybrid photovoltaic and battery energy storage system

This paper examines the cost analysis of a hybrid photovoltaic and battery energy storage system (PV-BESS) for demand-side applications in Southern Taiwan. The forms of input data and output data for three different invest plans are proposed and discussed. A sensitivity analysis to understand those affecting parameters to the investment is also investigated. The analysis results can support the users with a trade-off among economic, operational and environmental factors. This paper establishes a potential and practical tool for cost analysis of most energy conversion applications.
Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange

In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.
Transcranial Direct Current Stimulation (tDCS) Combined with Therapeutic Exercise and Cognitive Rehabilitation to Treat a Case of Burning Mouth Syndrome (BMS) Related Pain

Burning Mouth Syndrome (BMS) is a multifactorial, chronic pain condition with neuropathic and psychogenic mechanisms. Cortical activation as well as sustained attention and executive functions have proven to be affected by chronic pain. The main objectives of this work were to test the efficacy of a multidimensional personalized pain treatment protocol and to investigate if the effects are based on psychophysical pain processing changes or cognitive effects. A 74-year-old female with 2 years of BMS received 10 sessions of a combined protocol of anodal left dorsolateral prefrontal cortex tDCS, cognitive therapy, and therapeutic exercise. The subjective perception of pain decreased by 47% after treatment but returned to the baseline at 45 days. No changes were found in objective pain measurements apart from a transient worsening of conditioned pain modulation. A large effect size was found in all functional scales, processing speed and executive control as well as sustained attention that persisted during follow-up. No changes in anxiety and depression were found. A multimodal therapeutic approach combining TDCS, cognitive rehabilitation and therapeutic exercise produces improved quality of life, disability and pain perception correlated with improvements in processing speed, executive control and sustained attention but independent of changes in psychophysical pain processing.
Designing for Situation Awareness: An Approach to User-Centered Design

multilingual decision support systems. This is based upon knowledge management that uses context and ontology. Of strong interest to the Human Factors community is a chapter on acquiring knowledge from subject matter experts. It is a comprehensive chapter on the techniques and methodologies necessary for knowledge elicitation, followed by analysis and representation. Essentially this chapter is a succinct, constructive overview of the process and procedure of knowledge elicitation. On a theoretical standpoint is a chapter on fuzzy logic and its application to scientific reasoning. Potentially somewhat abstract, it is accessible in style and engaging and challenging, providing generous insight into fuzzy logic, without losing sight of its grasp of human scientific reasoning. Topically, a chapter on social networking research, particularly real-time online social networks (OSN) like Facebook and Flickr presents observations of knowledge based services, and within this the methods and techniques that allowed for the impact that real-life events had on, and are shaped by online activity within OSN’s (notably mentioned is the Arab Spring). The wealth of data about online usage – of individuals and groups – suggests a wealth of applications. Part IV discusses High Performance Knowledge Service Systems, with a section on smart phones and Web 2.0 describing how new technology has altered knowledge service, with further description of how knowledge transfer barriers have been lowered. This is cutting-edge evaluation of smart technology and its radical impact on knowledge service. Furthermore, cloud computing is described and models how data is transferred and manipulated, suggesting it is a form of data modelling. A business case for User-Centred Design is given – for people, process, culture and technology emphasises how the role of HFE and Usability can be engaged too late in a design process, imposing what we all know too give less meaningful change, or generating excessive cost to a project. Overall, the efforts of the authors are to explain and define knowledge as an attribute within the service sector of the economy. The collection of papers contained in the book provide insightful, descriptive and usable indications of current and projected reflections on technology, technology modelling and human considerations for the way knowledge is incorporated into systems. The chapters and sections provide sufficient overview, with sufficient depth to be a reasonable reference work for anyone seeking to understand the nature of knowledge as a resource.
Achievements and Challenges for Higher Education during the COVID-19 Pandemic: A Rapid Review of Media in Africa

The coronavirus (COVID-19) pandemic struck globally and has affected higher education institutions (HEIs) and their operations, indirectly impacting the progress of the Sustainable Development Goal 4 achieved thus far. This article addresses HEIs achievements and challenges experienced in the wake of the pandemic. Online news media reports played a facilitative role in providing information to the HEI communities. A rapid review exploring online news media messages relating to higher education at the onset of the COVID-19 pandemic in Africa was utilised. Narrative synthesis was used to analyse the data. The results highlight HEIs achievements, which aim to ensure that all students receive the same level of education and provision in terms of devices and mental health support. However, challenges were also experienced at HEIs and include students feeling uncertainty and fear regarding completing their education. Furthermore, the results also show that not all students received the same level of education due to contextual factors, thus deepening the existing social disparities in Africa. The pandemic provides an opportunity for HEIs to embed the components of global citizenship education into the curriculum and to work in an innovative way to promote Sustainable Development Goal 4.
Health Problems and the Use of Medications and Traditional Therapies among Chinese Immigrants Living in Spain

In this study, we investigate the health problems and the use of medications and traditional therapies among Chinese immigrants in the Southern region of Spain. A qualitative study using semi-structured interviews and including 133 immigrants and 7 stakeholders was conducted in 2017. Transcription, literal reading, and theoretical categorization were performed, and a narrative content analysis was carried out. The most common health problems were musculoskeletal (28.6%) and allergies (25.6%) related to work activity and unhealthy lifestyles. Key informants also reported gastric problems, stress, and changes in eating habits, mostly related to their work activity. For these problems, a large number of traditional remedies (herbs, diet therapy, acupuncture, vitamins, etc.) were used, usually combined with pharmaceutical drugs used for colds, flu, general malaise (29%), pain and fever (23%), and allergy drugs (9.2%). Chinese immigrants reported health conditions associated with their working conditions and life habits in Spain, using Traditional Chinese Medicine instead of pharmacological drugs. Understanding these health problems and promoting awareness towards traditional therapies in the healthcare system may help to design public policies and Health Promotion strategies targeting this group.
Mismatch repair and clinical response to immune checkpoint inhibitors in endometrial cancer

Endometrial cancer is common, and a subset recurs and requires additional treatment. Some of these are recognized as being susceptible to immune therapies and are said to have mismatch repair deficiency (dMMR). However, this clinical trial highlights which cases are more likely to respond well: those containing mutations in genes known as Lynch genes and also some with mutations in POLE/POLD1 (“ultra‐hypermutation” genes). In contrast, the majority of dMMR endometrial cancers have silencing or DNA methylation of one of these genes, MLH1, and do not seem to be as responsive to single‐agent immune therapy. The availability of combination therapies may be important to consider for these women.
Behaviour of Lightweight Concrete Wall Panel under Axial Loading: Experimental and Numerical Investigation toward Sustainability in Construction Industry

Awareness of sustainability in construction has led to the utilization of waste material such as oil palm shell (OPS) in concrete production. The feasibility of OPS as alternative aggregates in concrete has been widely studied at the material level. Meanwhile, nonlinear concrete material properties are not taken into account in the conventional concrete wall design equations, resulting in underestimation of lightweight concrete’s wall axial capacity. Against these sustainability and technical contexts, this research investigated the buckling behavior of OPS-based lightweight self-compacting concrete (LWSCC) wall. Failure mode, load-deflection responses, and ultimate strength were assessed experimentally. Numerical models have been developed and validated against experimental results. Parametric studies were conducted to study the influence of parameters like slenderness ratio, eccentricity, compressive strength, and elastic modulus. The results showed that the axial strength of concrete wall was very much dependent on these parameters. A generalized semi-empirical design equation, based on equivalent concrete stress block and modified by mathematical regression, has been proposed. The ratio of average calculated results to test results of the proposed equation, when compared to ACI 318, AS 3600, and Eurocode 2 equations, are respectively improved from 0.36, 0.31, and 0.42 to 0.97. This research demonstrates that OPS-based LWSCC concrete can be used for structural axial components and that the equation developed can serve a good guideline for its design, which could encourage automation and promote sustainability in the construction industry.
Risk Assessment of Insecticides Used in Tomato to Control Whitefly on the Predator Macrolophus basicornis (Hemiptera: Miridae)

Simple Summary The whitefly Bemisia tabaci is a problem in tomato crops worldwide. The use of chemicals is one method to control this pest. Predators from the family Miridae have been used in Europe as biological control agents. We tested the insecticides most often used to control B. tabaci in tomato fields in Brazil for compatibility with the native Brazilian mirid Macrolophus basicornis. The results showed that regarding lethality, buprofezin, cyantraniliprole and spiromesifen were reduced-risk insecticides. Acetamiprid, bifenthrin, etofenprox + acetamiprid and pyriproxyfen + acetamiprid were considered broad-spectrum insecticides. The insecticides were also tested to be classified ecologically and were found to be safe, except for acetamiprid that was moderately toxic. Overall, our findings indicated that it is possible to use M. basicornis as a biological agent to control B. tabaci in tomato crops by means of pest management strategies that are compatible with agrochemicals in current use. Abstract The generalist mirid predator Macrolophus basicornis may contribute to Integrated Pest Management (IPM) of Bemisia tabaci in tomato crops. It is important to know the compatibility of the chemicals used to control this pest with this promising biological control agent. Seven insecticides were tested to investigate their toxicity to the predator. For four of the products, the LC50 for adults were determined. Buprofezin, cyantraniliprole and spiromesifen did not cause lethality and were classified as harmless. Acetamiprid, bifenthrin, etofenprox + acetamiprid and pyriproxyfen + acetamiprid caused acute toxicity and were classified as harmful. LT50 for all harmful insecticides were relatively low, ranging from 1.8 to 3.2 days. Moreover, these four insecticides have low LC50, with acetamiprid (0.26 mg a.i. L−1) as the lowest, followed by bifenthrin (0.38 mg a.i. L−1), etofenprox + acetamiprid (4.80 mg a.i. L−1) and pyriproxyfen + acetamiprid (8.71 mg a.i. L−1). However, the calculated risk quotient (RQ) values demonstrated that these insecticides were mostly ecologically safe for this predator, except for acetamiprid, classified as slightly to moderately toxic. The present study can contribute to the use of M. basicornis as a biological control agent on tomato crops and to compatible use with the insecticides tested, according to IPM strategies.
Investigating Impact of Parental Motivations and Involvement within a Developing Country

This research investigated the effects of attitude and beliefs, subjective norms, and perceived behavioural control on parents’ home-based and school-based involvement in child education. The paper reports quantitative findings from a cross-sectional survey based on a concurrent mixed design. Primary data was collected from five hundred and sixty (560) parents who were selected randomly from deprived and non-deprived districts across six regions of Ghana. The Cronbach α of reliability for attitude and belief, perceived behavioural control, subjective norm, home-based and school-based involvement exceeded the minimum threshold. Results from structural equation modelling analysis based on a bootstrapping technique reveal that the model of the study was confirmed, as the data showed equivalence to model assessment measures including CFI = 0.979, TLI = 0.975, GFI = 0.942, AGFI = 0.922, RMSEA = 0.049, χ2/df = 2.292. The finding of the study indicated that attitude and beliefs, subjective norms, perceived behavioural control of parents significantly impact home-based involvement, whiles perceived behavioural control and subjective norms of parents had significantly impact on school-based involvement.
Widespread Distribution and Adaptive Degradation of Microcystin Degrader (mlr-Genotype) in Lake Taihu, China

Microbial degradation is an important route for removing environmental microcystins (MCs). Here, we investigated the ecological distribution of microcystin degraders (mlr-genotype), and the relationship between the substrate specificity of the microcystin degrader and the profile of microcystin congener production in the habitat. We showed that microcystin degraders were widely distributed and closely associated with Microcystis abundance in Lake Taihu, China. We characterized an indigenous degrader, Sphingopyxis N5 in the northern Lake Taihu, and it metabolized six microcystin congeners in increasing order (RR > LR > YR > LA > LF and LW). Such a substrate-specificity pattern was congruent to the order of the dominance levels of these congeners in northern Lake Taihu. Furthermore, a meta-analysis on global microcystin degraders revealed that the substrate-specificity patterns varied geographically, but generally matched the profiles of microcystin congener production in the degrader habitats, and the indigenous degrader typically metabolized well the dominant MC congeners, but not the rare congeners in the habitat. This highlighted the phenotypic congruence between microcystin production and degradation in natural environments. We theorize that such congruence resulted from the metabolic adaptation of the indigenous degrader to the local microcystin congeners. Under the nutrient microcystin selection, the degraders might have evolved to better exploit the locally dominant congeners. This study provided the novel insight into the ecological distribution and adaptive degradation of microcystin degraders.