Miracle-dz/UniFAR-FTUs huggingface dataset:
Beneficial Impact of Interspecies Chimeric Renal Organoids Against a Xenogeneic Immune Response

Background Animal fetal kidneys have the potential to be used as scaffolds for organ regeneration. We generated interspecies chimeric renal organoids by adding heterologous rat renal progenitor cells to single cells from mouse fetal kidneys and applying the renal development mechanism of mouse fetuses to rat renal progenitor cells to examine whether rat renal progenitor cells can differentiate into renal tissues of the three progenitor cell lineages of kidneys between different species. Furthermore, we investigated whether chimeric renal organoids with an increased proportion of recipient cells reduce xenogeneic rejection. Methods C57BL/6JJmsSlc mice (B6 mice) and Sprague-Dawley-Tg (CAG-EGFP) rat (GFP rats) fetuses were used as donors, and mature male NOD/Shi-scid, IL-2RγKO Jic mice (NOG mice) and Sprague-Dawley rats (SD rats) were used as recipients. First, fetal kidneys were removed from E13.5 B6 mice or E15.5 GFP rats and enzymatically dissociated into single cells. These cells were then mixed in equal proportions to produce chimeric renal organoids in vitro. The chimeric organoids were transplanted under the renal capsule of NOG mice, and maturation of the renal tissues in the organoids was observed histologically. Furthermore, chimeric organoids were prepared by changing the ratio of cells derived from mouse and rat fetal kidneys and transplanted under the renal capsule of SD rats subjected to mild immunosuppression to pathologically analyze the strength of the xenogeneic immune response. Results The cap mesenchyme was reconstructed in vitro, and nephron progenitor cells and ureteric buds were mosaically comprised GFP-negative mouse and GFP-positive rat cells. In the in vivo environment of immunodeficient mice, chimeric renal organoids mosaically differentiated and matured into renal tissues of three lineages. Chimeric renal organoids with high rates of recipient rat cells showed milder rejection than complete xenograft organoids. The vessels of recipient rats entered from the periphery of the transplanted chimeric renal organoids, which might reduce their immunogenicity. Conclusion Interspecies chimeric renal organoids may differentiate into mature renal tissues of each renal progenitor cell lineage. Furthermore, they may reduce transplant rejection compared with xenograft organoids.
The Crosstalk Between Saliva Bacteria and Fungi in Early Childhood Caries

Early childhood caries (ECC) is the most prevalent oral disease in children, which greatly affects the quality of life and health condition of the patients. Although co-infection of oral streptococci and fungi has been well recognized in the development of ECC, the correlation between other core members of oral mycobiome and ECC progression remains unclear. In the current study, saliva samples obtained from severe ECC (SECC), ECC, and caries-free children were collected, and both V3–V4 16S rRNA and ITS1 rRNA gene amplicon sequencing were performed to investigate the salivary bacterial and fungal profiles. Significant alteration of salivary fungal community in SECC/ECC children was observed compared with the caries-free control. The typing analysis determined the fungal community into five fungal types, which influenced the structure of salivary bacteria. By performing Spearman correlation analysis, carious phenotypes were positively related to Fusobacterium but negatively linked to Neocosmospora, and a significant correlation of cross-kingdom taxonomic pairs was identified. Our work demonstrated the interactions between oral bacteria and fungi at the community level, which may advance our knowledge on the etiological role of bacteria/fungi in the development of ECC and promote better management of this disease.
Chronic and Residual Effects of a Two-Week Foam Rolling Intervention on Ankle Flexibility and Dynamic Balance

Background Foam rolling has been shown to acutely improve joint range of motion (ROM). However, limited knowledge exists on the chronic and residual effects. The primary purpose of this study was to examine the chronic and residual effects of a 2-week roller–massager intervention on ankle dorsiflexion ROM and dynamic balance. Methods Forty-two participants (24.3 ± 2.5 years, 33 males, 9 females) were randomly assigned to either roller-massage (RM) or control group (= no intervention). Ankle ROM was assessed with the weight-bearing lunge test (WBLT) and dynamic balance with the Y-Balance test for both limbs. The RM group was instructed to roll their calf muscles for three sets of 60 s per leg on 6 days a week over 2 weeks. Acute effects were measured during baseline testing for dorsiflexion ROM and dynamic balance immediately after foam rolling. Chronic and residual effects were measured 1 day and 7 days after the intervention period. Multivariate ANOVA was performed for post-hoc comparisons to determine acute, chronic, and residual effects. Results Significant acute and chronic foam rolling effects (p <0.05) were found for ankle dorsiflexion ROM. The chronic increase in ROM slightly decreased 7 days post-intervention but remained significantly above baseline (p < 0.05). Regarding dynamic balance, there were no acute but chronic (p < 0.05) and residual (p < 0.05) effects. Conclusion Using a roller–massager for a 2-week period chronically increases ROM and dynamic balance. These increases are still significant 7 days post-intervention emphasizing the sustainability of foam rolling effects.
Comparative Analysis of Intestinal Microflora Between Two Developmental Stages of Rimicaris kairei, a Hydrothermal Shrimp From the Central Indian Ridge

Despite extreme physical and chemical characteristics, deep-sea hydrothermal vents provide a place for fauna survival and reproduction. The symbiotic relationship of chemotrophic microorganisms has been investigated in the gill of Rimicaris exoculata, which are endemic to the hydrothermal vents of the Mid-Atlantic Ridge. However, only a few studies have examined intestinal symbiosis. Here, we studied the intestinal fauna in juvenile and adult Rimicaris kairei, another species in the Rimicaris genus that was originally discovered at the Kairei and Edmond hydrothermal vent fields in the Central Indian Ridge. The results showed that there were significant differences between juvenile and adult gut microbiota in terms of species richness, diversity, and evenness. The values of Chao1, observed species, and ASV rarefaction curves indicated almost four times the number of species in adults compared to juveniles. In juveniles, the most abundant phylum was Deferribacterota, at 80%, while in adults, Campilobacterota was the most abundant, at 49%. Beta diversity showed that the intestinal communities of juveniles and adults were clearly classified into two clusters based on the evaluations of Bray–Curtis and weighted UniFrac distance matrices. Deferribacteraceae and Sulfurovum were the main featured bacteria contributing to the difference. Moreover, functional prediction for all of the intestinal microbiota showed that the pathways related to ansamycin synthesis, branched-chain amino acid biosynthesis, lipid metabolism, and cell motility appeared highly abundant in juveniles. However, for adults, the most abundant pathways were those of sulfur transfer, carbohydrate, and biotin metabolism. Taken together, these results indicated large differences in intestinal microbial composition and potential functions between juvenile and adult vent shrimp (R. kairei), which may be related to their physiological needs at different stages of development.
Application of Solubility and Dissolution Profile Comparison for Prediction of Gastric pH-Mediated Drug-Drug Interactions

The objective of this study was to assess how solubility and dissolution profile comparisons under different pH conditions can be used to predict gastric pH-mediated drug-drug interaction (DDI) potential. We collected information for new molecular entities (NMEs) approved from 2003 to 2019 by the U.S. Food and Drug Administration (FDA) that had dedicated clinical DDI studies with acid-reducing agents (ARAs). Among these, 67 NMEs with solubility under different pHs and dissolution profiles generated in pH 1.2, 4.5, and 6.8 aqueous media were included for analysis. Similarity factor (f2) was used to compare dissolution profiles at different pHs for pH-mediated DDI prediction (e.g., f2<50 predicts positive DDI). Prediction accuracy was calculated based on the outcome comparison between predicted and observed DDIs. Based on dissolution profile comparisons and observed DDI data, weak base drugs (WBDs) (n = 49) showed 72.5% prediction accuracy under the fasted conditions, and 66.7% prediction accuracy under fed conditions. While using solubility and clinical dose for prediction, the prediction accuracy was 80% under fasted conditions and 66.7% under fed conditions, respectively. Comparison of dissolution profiles generated at pH 1.2, 4.5, and 6.8 can be used to predict gastric pH-mediated DDI potential for WBDs. It demonstrated comparable prediction accuracy under both fasted and fed conditions when compared to the prediction using solubility and clinical dose. Furthermore, dissolution profile comparison could add an additional understanding of possible impact of pH change on the release behavior of the drug product. Graphical abstract Graphical abstract
Knowledge, attitude and practice of community-dwelling adults regarding advance care planning in Malaysia: a cross-sectional study

Objectives This study aimed to assess the knowledge, attitude and practice (KAP) among community-dwelling adults in Malaysia regarding advance care planning (ACP), and its associated factors. Design This cross-sectional study was conducted from July–September 2018. Setting This study was conducted at the University Malaya Medical Centre, Kuala Lumpur, Malaysia. Participants We recruited community-dwelling adults (ambulatory care patients or their accompanying persons) who were ≥21 years old and able to understand English or Malay. A 1:10 systematic sampling procedure was used. Excluded were community-dwelling adults with intellectual disabilities or non-Malaysian accompanying persons. A trained researcher administered the validated English or Malay Advance Care Planning Questionnaire at baseline and 2 weeks later. Primary and secondary outcome measures The primary outcome was the KAP regarding ACP. The secondary outcomes were factors associated with KAP. Results A total of 385/393 community-dwelling adults agreed to participate (response rate 98%). Only 3.1% of the community-dwelling adults have heard about ACP and 85.7% of them felt that discussion on ACP was necessary after explanation of the term. The desire to maintain their decision-making ability when seriously ill (94.9%) and reducing family burden (91.6%) were the main motivating factors for ACP. In contrast, resorting to fate (86.5%) and perceived healthy condition (77.0%) were the main reasons against ACP. Overall, 84.4% would consider discussing ACP in the future. Community-dwelling adults who were employed were less likely to know about ACP (OR=0.167, 95% CI 0.050 to 0.559, p=0.004) whereas those with comorbidities were more likely to favour ACP (OR=2.460, 95% CI 1.161 to 5.213, p=0.019). No factor was found to be associated with the practice of ACP. Conclusions Despite the lack of awareness regarding ACP, majority of community-dwelling adults in Malaysia had a positive attitude towards ACP and were willing to engage in a discussion regarding ACP after the term ‘ACP’ has been explained to them.
Modeling and forecasting of wheat in India and their yield sustainability

Forecasting production behaviours of the major crops play vital role towards the planners for food and nutritional security. The planners should have idea about the likely production scenario of the major crops. Wheat and rice are the two major staple food crops in Indian subcontinent. As such performance of wheat production is largely associated with food security of this continent. In this paper attempt has been made to examine the performance of wheat production in India and its major states during the 1950–2009. This study also focuses on forecasting the cultivated area and production of wheat in India using time series modelling technique using Autoregressive Integrated Moving Average (ARIMA) model. The success of agriculture depends on so many factors from formulation of policy to its implementation, availability of inputs, climatic conditions etc. Effective forecasting process should take all these factors into consideration. Instead of using uni-variate time trend ARIMA models, the study feels inclusion of factors of production may improve the quality as external exogenous predictors well as forecasting power of the model.
The Effect of Corporate Social Responsibility (CSR) Program and Company Image on Customer Loyalty PT. PMN

The purpose of this study is to explore the impact of corporate social responsibility (CSR) PT. Civil Investment (PNM-Persero) and the company's image on customer satisfaction, and how it can shape PNM customer loyalty. The types of data used are primary data and secondary data. The population for this study were customers of PT. PNM in the East Bandung area. The sample taken is using the Slovin formula and snowball sampling technique so that 100 respondents are obtained. Partial Least square (PLS) analysis method uses SPSS 23 and descriptive analysis method uses cross tabulation. The results of the analysis show that there is a positive effect of CSR activities and corporate image on customer satisfaction and loyalty. Based on the research results, CSR activities have proven to be the most important factor compared to other factors in increasing customer
A Prospective Study to Examine the Clinical and Laboratory Characteristics of Typhoid Fever in Children Under 18 Years Age in Bihar Region

Background: Enteric fever, also known as typhoid, is a common worldwide bacterial disease caused by the ingestion of contaminated food or water which contains the bacterium Salmonella enterica, serovar Typhi. It disproportionately affects children with varied clinical presentations ranging from a mild illness with low grade fever to severe life-threatening complications. Aim: To evaluate clinical and laboratory profile of typhoid fever in children of Bihar region. Materials and Methods: Total 150 children aged below 18 years who presented in 2 years to the Department of Pediatrics, Patna Medical College and Hospital, Patna, Bihar, India with history of fever of more than 7-10 days duration were included in this study. In each case, age, sex, presenting complaint, past medical history, laboratory investigations including Widal test and antibiotic sensitivity pattern are collected and analyzed. Results: Out of sample population of 150, 90 (60 %) were males and 60 (40 %) were females. Out of all these patients, maximum cases (58 %) were from age group of 7-12 years of age. The most common symptoms were fever (100 %), followed by anorexia (71.33 %). We observed high incidence of typhoid fever in lower class (28.67 %) and lower middle class (48 %), lesser in upper middle-class society (14 %) and least in upper class (9.33 %). ]. The most common physical findings found in our study was toxic look (68.67 %) followed by coated tongue (62.67 %) and hepatomegaly (40 %). Conclusion: Typhoid fever should be suspected and investigated in all children with short and long duration fever without localizing signs. Early diagnosis and institution of appropriate antibiotics therapy is of paramount importance in the management of typhoid fever.
The Moderating Role of Emotional Intelligence on the Relationship Between Job Resources and Employee Engagement

THE MODERATING ROLE OF EMOTIONAL INTELLIGENCE ON THE RELATIONSHIP BETWEEN JOB RESOURCES AND EMPLOYEE ENGAGEMENT By Amanda Lipson The purpose of the current study was to examine the moderating role of emotional intelligence on the relationship between job resources (i.e., perceived supervisor support and autonomy) and employee engagement. Previous research has shown that these job resources are related to employee engagement. However, little attention has been paid to the possible moderators of this relationship. Therefore, this study aimed to bridge the gap in current literature by investigating how the relationship between job resources and engagement might change as a function of a personal resource (emotional intelligence). It was hypothesized that the positive relationship between job resources (perceived supervisor support and autonomy) and employee engagement would be stronger for employees with high emotional intelligence than for those with low emotional intelligence. A total of 125 employed individuals from various industries participated in an online survey. Consistent with the literature, both perceived supervisor support and autonomy had strong positive relationships with engagement. However, emotional intelligence did not moderate the relationship between supervisor support and autonomy and employee engagement. It is suggested that companies educate and train their leaders on the importance and practice of providing their employees with supervisor support and autonomy in the workplace.
Rainwater quality from roofs, tanks and hot water systems at figtree place

Figtree Place is a water sensitive urban redevelopment consisting of27 residential units located in Hamilton, an inner suburb of Newcastle, NSW, Australia. The site uses rainwater stored in tanks to supply hot water and toilet flushing demand. A two-year monitoring program for roofwater, tanks and hot water systems revealed that water quality improves in the roof to tank to hot water system treatment chain. Analysis of water quality from hot water systems and rainwater tanks showed compliance with the Australian Drinking Water Guidelines.
THE INFLUENCE OF MACHIAVELLIAN TRAITS, IN MODERATING THE RELATIONSHIP OF TASK COMPLEXITY, AND LOCUS OF CONTROL AS ANTECEDENTS OF AUDITOR DYSFUNCTIONAL BEHAVIOR IN PUBLIC ACCOUNTING FIRMS

This study aims to determine the effect of task complexity as an antecedent of auditor dysfunctional behavior, locus of control as an antecedent of auditor dysfunctional behavior, machiavellian nature as a moderating variable capable of influencing task complexity as an antecedent of auditor dysfunctional behaviour, Machiavellian nature as a Moderating Variable can influence the Locus Of Control as an Antecedent of Auditor Dysfunctional Behavior. This research includes including associative research. The population in this study was 70 internal auditors who worked at the Public Accounting Firm in Bali Province. This research uses instrument tests, classical assumption tests, F statistic tests, and hypothesis tests. The data analysis technique used is Moderated Regression Analysis. The results of this study indicate that Task Complexity has a positive and significant effect as an antecedent of Auditor Dysfunctional Behavior at the Bali Province Public Accountant Office. The Locus Of Control variable has a significant effect as an Antecedent of Auditor Dysfunctional Behavior. The moderating Machiavellian trait does not affect Task Complexity as Antecedent of Auditor Dysfunctional Behavior. Machiavellian nature moderates the effect of Locus Of Control as Antecedent of Auditor Dysfunctional Behavior at the Bali Province Public Accountant Office.
A climatology of supercooled layer clouds from lidar ceilometer data

It was demonstrated during the Cloud Lidar And Radar Experiment (CLARE’98) that supercooled water in the atmosphere can occur in the form of distinct layers several hundred metres thick that provide a strong signal for lidar, but because of the small droplet size are undetected by radar when ice is present (e.g. Hogan et al. 1999). The fact that these layers give such a strong echo at visible wavelengths implies that, when present, they are much more important in determining the radiative properties of the cloud than any ice that may be around. They will also be important in glaciation processesand therefore have an impact on cloud lifetime and precipitation formation. Currently, atmospheric forecast and climate models usually assume a simple ratio between ice and liquid water content that varies with temperature only; Fig. 1 shows this ratio for the ECMWF model. Clearly if such layers are common then there is a need to improve the representation of supercooled water in models. Intensive observing periods, such as during CLARE’98, have served to demonstrate the existence of these layers and establish that supercooled liquid water is responsible. However, long-term measurements are required to determine their frequency and mean properties, ultimately what model climatologies would be tested against. In this paper a first attempt is made to characterise the frequency of supercooled layers using data taken from a 905 nm Vaisala CT75K lidar ceilometer, which has been operating almost continuously at Chilbolton, England since June 1996. Figure 2 shows a time-height section of lidar backscatter coefficient (β) from this instrument (resolution 30 metres and 30 seconds) through a typical layer with a temperature of around −20◦C, on 15 October 1998 during
#VACHINA: HOW POLITICIANS HELP TO SPREAD DISINFORMATION ABOUT COVID-19 VACCINES

This paper focuses on how Brazilian politicians helped to spread disinformation about Covid-19 vaccines, discussing legitimation strategies and actors that played a significant role on Twitter and Facebook. Based on data gathered through CrowdTangle and Twitter API, we selected the 250 most shared/retweeted posts for each dataset (n=500) and examined if they contained disinformation, who posted it, and what strategy was used to legitimize this discourse. Our findings indicate that politicians and hyperpartisan accounts have a key influence in validating the Brazilian president’s populist discourse through rationalization (pseudo-science) and denunciation (against the vaccine). The political frame also plays an important role in disinformation messages.
Impact of CEO overconfidence on corporate financing decision Impact of CEO overconfidence on corporate financing decision with mediating role of risk perception with mediating role of risk perception

The purpose of this paper is to investigate the impact of managerial (Chief Executive Oﬃcer) overconﬁdence on corporate ﬁnancing decision with the mediating role of risk perception. This study indicates that psychological biases aﬀect the risk perception and ultimately ﬁnancing decision of top management of an organization. The research design is causal and primary data has been used to test the proposed relationship. Among all the companies listed in the Pakistan Stock Exchange, researchers have selected top executives of 200 companies as a sample. E-Questionnaire has been used to collect the required data through LinkedIn and other mailing sources. Researcher has done a linear regression to ﬁnd the relationship between independent variable overconﬁdence and dependent variable that is leverage. Baron and Kennys four-step mediation has been used to test the mediation eﬀect of risk perception. Results of this study conclude that there is a signiﬁcant positive relationship between overcon-ﬁdence of CEO and leverage while there is a signiﬁcant negative relationship between CEO overconﬁdence and risk perception. However, there is an insigniﬁ-cant result for the mediating role of risk perception between CEO overconﬁdence and leverage. Extensive research work is available on basic topic of how divi-dend policy and capital structure aﬀect corporate performance; however, we found insuﬃcient literature on psychological forces (behavioural biases) for ﬁ-nancial decision making. The gap is covered by this study through explaining the impact of behavioural biases on corporate ﬁnancial decisions which further distresses corporate performance. The results are signiﬁcant for company top management, regulators and policy makers. This study as an empirical evidence is also helpful for researchers, academicians, and practitioners to understand and