Miracle-dz/UniFAR-FTUs huggingface dataset:
Trypanosoma evansi infection in a captive Indian Wolf Canis lupus pallipes – molecular diagnosis and therapy

A five-year old, apparently healthy male Indian Wolf Canis lupus pallipes of Nandankanan Zoological Park, Odisha became ill with acute signs of anorexia, lethargy, staggering gait, and was non-responsive to external stimuli. Microscopic examination of Giemsa stained blood smear revealed presence of extracellular flagellates having morphological similarity to Trypanosoma spp. Haematological parameters showed anaemia (Hb 6.0 g%), mild leucopenia (total leukocyte count 5 × 103 / mm3) and thrombocytopenia (180 x 103 / µl). Serum biochemistry revealed high aspartate aminotransferase (AST) (830 IU/L), blood urea nitrogen (BUN) (178.2 mg/dl), creatinine (4.44 mg/dl), and low glucose (25.7 mg/dl) levels. Polymerase chain reaction (PCR) analysis targeting internal transcribed spacer (ITS1) region followed by National Centre for Biotechnology Information blast confirmed Trypanosoma evansi infection in the captive Indian Wolf. The animal showed clinical recovery with the administration of single dose of quinapyramine sulphate and quinapyramine chloride @ 4.0 mg/kg b wt subcutaneously. The wolf started taking meat from the very next day with improved activity. No trypanosomes could be detected in the stained blood smears as well as through PCR carried 25 days post treatment. The occurrence became an eye opener for the zoo and henceforth, all canids were included under chemoprophylaxis protocol against trypanosomosis.
Slope position and biochar influence soil properties and seed displacement in a tropical agroecosystem

Soil erosion due to land use change is a major concern in agricultural areas. There is an urgent need to develop appropriate agricultural practices that promote higher biodiversity and improve soil properties. Little is known about the processes of natural vegetation recovery after disturbance by human activities in tropical humid climates. This is particularly the case for dispersion by water (hydrochory) that affects seeds. The displacement of native plant seeds on the soil surface by runoff may be influenced by soil properties and by the use of biochar in agriculture. As these effects have not been documented in tropical agroecosystems and on steep slopes, we present here an in‐field experiment on a steeply sloping maize field affected by rainfall and tillage erosion in Northern Thailand. Our objectives were to test the effect of catena position and biochar use on seed displacement, soil loss and nutrient losses. We used 24 plots of 1 m2 located at four positions in the catena. Two treatments were tested: (1) conventional system with tillage and (2) biochar incorporated into the soil. We measured the displacement of plastic beads (to imitate round seeds), runoff volume, soil and nutrient losses and soil surface features during in‐situ rainfall simulations. After two simulated rainfall events and four natural rainfall events, total bead exportation was 7.2%. We found that catena position and biochar had an impact on runoff, soil and nutrient losses. In this study, previously incorporated biochar reappeared at the soil surface after 8 months under natural conditions and acted like a crust at the soil surface. This biochar crust increased runoff, increased potassium concentration and decreased phosphorus concentration in runoff, decreased bead displacement and had no effect on soil loss. This study, under tropical climate and steep slope conditions, highlights differences in soil surface features and runoff along the catena. We should consider catena position for improving soil management and using appropriate agroecological practices.
Magnetic Fields and Cancer: Epidemiology, Cellular Biology, and Theranostics

Humans are exposed to a complex mix of man-made electric and magnetic fields (MFs) at many different frequencies, at home and at work. Epidemiological studies indicate that there is a positive relationship between residential/domestic and occupational exposure to extremely low frequency electromagnetic fields and some types of cancer, although some other studies indicate no relationship. In this review, after an introduction on the MF definition and a description of natural/anthropogenic sources, the epidemiology of residential/domestic and occupational exposure to MFs and cancer is reviewed, with reference to leukemia, brain, and breast cancer. The in vivo and in vitro effects of MFs on cancer are reviewed considering both human and animal cells, with particular reference to the involvement of reactive oxygen species (ROS). MF application on cancer diagnostic and therapy (theranostic) are also reviewed by describing the use of different magnetic resonance imaging (MRI) applications for the detection of several cancers. Finally, the use of magnetic nanoparticles is described in terms of treatment of cancer by nanomedical applications for the precise delivery of anticancer drugs, nanosurgery by magnetomechanic methods, and selective killing of cancer cells by magnetic hyperthermia. The supplementary tables provide quantitative data and methodologies in epidemiological and cell biology studies. Although scientists do not generally agree that there is a cause-effect relationship between exposure to MF and cancer, MFs might not be the direct cause of cancer but may contribute to produce ROS and generate oxidative stress, which could trigger or enhance the expression of oncogenes.
Similar Solutions for Free Convection From a Nonisothermal Vertical Plate

An analysis is made for laminar free convection from a vertical flat plate having a nonuniform surface temperature. The following two families of surface temperatures were studied: (i) Tw – T∞ = Nxn, (ii) Tw – T∞ = Memx. Both families submit to mathematical analysis by the conventional techniques of laminar boundary-layer theory; i.e., they permit the finding of similar solutions of the boundary-layer equations. Numerical solutions have been carried out for Pr = 0.7 and 1.0; i.e., for gases. Heat-transfer results are presented, as are temperature and velocity distributions.
Individualized Dosage Optimization for Myeloablative Conditioning before Unrelated Cord Blood Transplantation in a Diamond–Blackfan Anemia Patient with Germline RPL11 Mutation: A Case Study

Unrelated cord blood transplantation (CBT) for Diamond–Blackfan anemia (DBA), a systemic ribosomopathy affecting the disposition of conditioning agents, has resulted in outcomes inferior to those by transplantations from matched donors. We report the experience of the pharmacokinetics-guided myeloablative unrelated CBT in a DBA patient with a germline RPL11 mutation. The conditioning consisted of individualized dosing of fludarabine (based on weight and renal function with a target area under the curve (AUC) of 17.5 mg·h/L) and busulfan (based on therapeutic drug monitoring with a target AUC of 90 mg·h/L), as well as dosing and timing of thymoglobulin (based on body weight and pre-dose lymphocyte count to target pre-CBT AUC of 30.7 AU·day/mL and post-CBT AUC of 4.3 AU·day/mL, respectively). The pharmacokinetic measures resulted in a 27.5% reduction in busulfan and a 35% increase in fludarabine, as well as an over three-fold increase in thymoglobulin dosage with the start time changed to day-9 instead of day-2 compared to regular regimens. The transplantation resulted in rapid, complete, and sustained hematopoietic engraftment. The patient is now healthy over 3 years after CBT. A pharmacokinetics-guided individualized dosing strategy for conditioning might be a feasible option to improve the outcomes of DBA patients receiving unrelated myeloablative CBT.
Microfluidic Compartmentalization Platforms for Single Cell Analysis

Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis.
Dielectric Properties of Colossal-Dielectric-Constant Na1/2La1/2Cu3Ti4O12 Ceramics Prepared by Spark Plasma Sintering

In the current study, we report on the dielectric behavior of colossal-dielectric-constant Na1/2La1/2Cu3Ti4O12 (NLCTO) ceramics prepared by mechanochemical synthesis and spark plasma sintering (SPS) at 850 °C, 900 °C, and 925 °C for 10 min. X-ray powder diffraction analysis showed that all the ceramics have a cubic phase. Scanning electron microscope observations revealed an increase in the average grain size from 175 to 300 nm with an increase in the sintering temperature. SPS NLCTO ceramics showed a room-temperature colossal dielectric constant (>103) and a comparatively high dielectric loss (>0.1) over most of the studied frequency range (1 Hz–40 MHz). Two relaxation peaks were observed in the spectra of the electrical modulus and attributed to the response of grain and grain boundary. According to the Nyquist plots of complex impedance, the SPS NLCTO ceramics have semiconductor grains surrounded by electrically resistive grain boundaries. The colossal dielectric constant of SPS NLCTO ceramics was attributed to the internal barrier layer capacitance (IBLC) effect. The high dielectric loss is thought to be due to the low resistivity of the grain boundary of SPS NLCTO.
In Vivo Biocompatibility Investigation of an Injectable Calcium Carbonate (Vaterite) as a Bone Substitute including Compositional Analysis via SEM-EDX Technology

Injectable bone substitutes (IBS) are increasingly being used in the fields of orthopedics and maxillofacial/oral surgery. The rheological properties of IBS allow for proper and less invasive filling of bony defects. Vaterite is the most unstable crystalline polymorph of calcium carbonate and is known to be able to transform into hydroxyapatite upon contact with an organic fluid (e.g., interstitial body fluid). Two different concentrations of hydrogels based on poly(ethylene glycol)-acetal-dimethacrylat (PEG-a-DMA), i.e., 8% (w/v) (VH-A) or 10% (w/v) (VH-B), were combined with vaterite nanoparticles and implanted in subcutaneous pockets of BALB/c mice for 15 and 30 days. Explants were prepared for histochemical staining and immunohistochemical detection methods to determine macrophage polarization, and energy-dispersive X-ray analysis (EDX) to analyze elemental composition was used for the analysis. The histopathological analysis revealed a comparable moderate tissue reaction to the hydrogels mainly involving macrophages. Moreover, the hydrogels underwent a slow cellular infiltration, revealing a different degradation behavior compared to other IBS. The immunohistochemical detection showed that M1 macrophages were mainly found at the material surfaces being involved in the cell-mediated degradation and tissue integration, while M2 macrophages were predominantly found within the reactive connective tissue. Furthermore, the histomorphometrical analysis revealed balanced numbers of pro- and anti-inflammatory macrophages, demonstrating that both hydrogels are favorable materials for bone tissue regeneration. Finally, the EDX analysis showed a stepwise transformation of the vaterite particle into hydroxyapatite. Overall, the results of the present study demonstrate that hydrogels including nano-vaterite particles are biocompatible and suitable for bone tissue regeneration applications.
Defeating Misclassification Attacks Against Transfer Learning

Transfer learning is prevalent as a technique to efficiently generate new models (Student models) based on the knowledge transferred from a pre-trained model (Teacher model). However, Teacher models are often publicly available for sharing and reuse, which inevitably introduces vulnerability to trigger severe attacks against transfer learning systems. In this article, we take a first step towards mitigating one of the most advanced misclassification attacks in transfer learning. We design a distilled differentiator via activation-based network pruning to enervate the attack transferability while retaining accuracy. We adopt an ensemble structure from variant differentiators to improve the defence robustness. To avoid the bloated ensemble size during inference, we propose a two-phase defence, in which inference from the Student model is first performed to narrow down the candidate differentiators to be assembled, and later only a small, fixed number of them can be chosen to validate clean or reject adversarial inputs effectively. Our comprehensive evaluations on both large and small image recognition tasks confirm that the Student models with our defence of only 5 differentiators are immune to over 90% of the adversarial inputs with an accuracy loss of less than 10%. Our comparison also demonstrates that our design outperforms prior problematic defences.
Evaluation of Epidemiology and Etiologies of Acute Limping in Children Presented to the Emergency Department of Pediatric Hospital of Amirkola in Babol, Iran

Background: Limping is a common complaint of children presenting to the emergency departments of hospitals; therefore, it is important to identify its causes and avoid unnecessary measures. In this study, common etiologies for limping in children presenting to the emergency department of Pediatric Hospital of Amirkola in Babol, Iran were evaluated. Methods: All children under 15 years of age presented to the emergency department of Pediatric Hospital of Amirkola, during 2017-2018, with complaints of acute limping were retrospectively evaluated. Initially, 71 patients were found among whom 53 were selected based on inclusion and exclusion criteria of the study. The census method was used to obtain the required samples. Patient information including demographic data, clinical signs, and symptoms at admission were gathered. Statistical analysis was done using SPSS v22 software (IBM, USA), and p<0.05 was considered statistically significant. Results: Of 53 children, 33 (62.3%) were boys and 20 (37.7%) were girls. The mean age of participants was 4.53±2.79 years old, and the mean length of hospital stay was 10.5±7.2 days. Inflammation and infections were the most common etiologies with a cumulative prevalence of 58.49 and 28.30%, respectively. Transient synovitis was the most common differential diagnosis with a prevalence of 43.4%, and the most affected joints were hips with a prevalence of 73.5% (p-values=0.001). Conclusion: Most of the diseases for acute limping in children are self-limited, but this should not lead to disregard for infection as a differential diagnosis.
Association of P450 Oxidoreductase Gene Polymorphism with Tacrolimus Pharmacokinetics in Renal Transplant Recipients: A Systematic Review and Meta-Analysis

There are conflicting results regarding the effect of the P450 oxidoreductase (POR) *28 genotype on the tacrolimus (TAC) pharmacokinetics (PKs) during the early post-transplantation period in adult renal transplant recipients. Thus, we characterized the impact of POR*28 on TAC PKs. We conducted a systematic review on the association between POR*28 and PKs of TAC in adult renal transplant recipients. Structured searches were conducted using PubMed, Web of Science, and Embase. TAC standardized trough concentration (ng/mL per mg/kg) data were extracted. Mean differences (MD) and their corresponding 95% confidence intervals (CIs) were used to identify the differences between the POR*28 genotype and PKs of TAC. The subgroup analysis was conducted according to CYP3A5 expression status. Six studies (n = 1061) were included. TAC standardized trough concentrations were significantly lower in recipients with the POR*28 allele compared to recipients with POR*1/*1 (MD: 8.30 ng/mL per mg/kg; 95% CI: 1.93, 14.67; p = 0.01). In the subgroup analysis, TAC standardized trough concentrations were lower for subjects who were POR*28 carriers than those who were POR*1/*1 in CYP3A5 expressers (MD: 20.21 ng/mL per mg/kg; 95% CI: 16.85, 23.56; p < 0.00001). No significant difference between POR*28 carriers and POR*1/*1 was found in the CYP3A5 non-expressers. The results of our meta-analysis demonstrated a definite correlation between the POR*28 genotype and PKs of TAC. Patients carrying the POR*28 allele may require a higher dose of TAC to achieve target levels compared to those with POR*1/*1, especially in CYP3A5 expressers.
Static and dynamic testing of steel wire mesh for mining applications of rock surface support

Wire mesh has been used for surface ground support in mining since the 1950s. Experimental studies have been conducted throughout the world. The response of the mesh is dependant on the sample size, the boundary restraint systems and the loading conditions. The Western Australian School of Mines (WASM) has developed a facility for the static testing of ground support elements. The static facility consists of several stiff steel frames used to support the sample and a screw feed jack used to load the sample. The sample is restrained on all sides and displaced at a constant rate by a screw feed jack. Instrumentation measures the displacement and the load being applied. The WASM dynamic test facility has been recently modified to enable the testing of surface support elements. Surface support element testing at the dynamic test facility will be conducted using sample sizes and boundary conditions similar to the static testing facility to enable the comparison of results between the two facilities. Two testing programs have been undertaken to assess the static and dynamic performance of welded wire and chain link mesh. The results of the test programs are presented and comparisons are made for the performance of the two types of mesh.
Perceptions and practices of fundamental movement skills in grassroots soccer coaches

This study presents the perceptions and practices of fundamental movement skills (FMS) in grassroots soccer coaches. One hundred and twenty-eight coaches (123 males, 5 females) completed an online mixed-method survey comprising 32 questions relating to: participant demographics, education, and qualifications; FMS perceptions, practices, and assessments, and the importance of FMS constructs; and other factors related to FMS. Frequency analysis was used to assess and report responses to fixed response and Likert-scale questions, and thematic analysis used for open-ended questions. Results indicated that grassroots soccer coaches have an awareness of the concept of FMS and value FMS as a contributor to developing general movement and soccer specific skills. However, there was a tendency for the coaches to conflate FMS with fitness. Coaches in the current study reported that developing FMS was useful to improve soccer development. The coaches suggested they assessed FMS but the measures they employed predominantly focused on more general movement outcomes. No coach used a valid or reliable process-oriented FMS assessment. Coaches used resources to inform their practice for FMS development, but the quality of resources accessed lacked an evidence base, with a reliance on social media. While the coaches in the current study reported valuing FMS, there are gaps in coach education and available evidence-based resources which inhibit the effective development of FMS within grassroots soccer practice. Providing training, qualifications and additional support for coaches related to FMS will aid implementation in practice.
Public Services, Ombudsman, and State Law-Democracy: Political and Organizational Analysis

The establishment of the Ombudsman with Law No. 37 of 2008 and Law No. 25 of 2009 on Public Services are important for Indonesia as a legal-democracy state. The regulation on the organizational dimensions in the law is inadequate. With normative juridical methods and literature, it is clear that the Ombudsman as an independent state institution does not have organic relations with state institutions, but is politically less than optimal in carrying out its functions. Its role is very strategic, in supervising the implementation of public services, and in accordance with one of its objectives, namely realizing a democratic, just, and prosperous legal state. The establishment of an Ombudsman representative at the provincial and/or district/city level according to Article 5 paragraph (2) and Article 43 paragraph (1) of Law Number 37 of 2008 is not imperative. However, according to Article 46 paragraph (3) of Law Number 25 of 2009, the Ombudsman is obliged to form representatives in the regions to support their duties and functions in public services. Politically, not mentioning the nine members of the Ombudsman as state officials has the potential to reduce the weight of their institutions as state institutions. It is necessary to improve the regulation of several organizational dimensions for institutional strengthening within the framework of a legal-democracy state.
‘Enter parliament but never become part of it’: How have the Greens in the United Kingdom approached opposition?

The Greens in the United Kingdom have benefitted from the fragmentation of the party system and the creation of devolved institutions, achieving consistent representation at multiple levels of British politics in recent years. However, we know little about what they have done in these positions. This study uses interviews with Green legislators to investigate how they have interpreted the task of opposition at Westminster, Holyrood and Stormont. We show that Green legislators’ approaches to opposition have been influenced by their party identity, and that differences in approach between the institutions have largely been determined by contrasting political opportunity structures, rather than parliamentary rules. These findings highlight the importance of party as a factor shaping opposition strategy and the varied roles which opposition parties can play within the UK political system.