Miracle-dz/UniFAR-FTUs huggingface dataset:
Semiochemicals Affecting Attraction of Ambrosia Beetle Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) to Quercivorol: Developing Push-Pull Control

Abstract Euwallacea fornicatus (Eichhoff), the polyphagous shot hole borer (PSHB), is an ambrosia beetle infesting avocado Persea americana Mill. limbs in North America and Israel. We conducted field experiments with sticky traps in avocado orchards to develop push–pull semiochemical methods of managing PSHB. Traps baited with 10-fold increasing doses (0.01 to 100× or 1.26 µg to 12.6 mg/d) of attractant quercivorol were previously shown to increasingly capture female PSHB (males flightless). We converted trap catch of this relationship to a standardized effective attraction radius (EAR) that predicts capture power of baited-traps regardless of insect flight density. Earlier, piperitone and verbenone were shown to strongly inhibit attraction of PSHB to quercivorol-traps. We tested increasing numbers of 1× piperitone dispensers at 0.75-m distance surrounding a quercivorol-trap and found PSHB catch to decline exponentially. Increasing decadic doses (0.01 to 10×) of either verbenone or piperitone released at 1× quercivorol-traps caused a sigmoidal first-order kinetic-decay in catch. Verbenone (1×) placed at increasing distances (0, 0.25, 0.5, 1, and 2 m) from a 1× quercivorol-trap became increasingly ineffective in reducing catch of PSHB. We found no evidence that ethanol released from 7.5 to 480 mg/d affected attraction of PSHB, but Scobicia chevrieri (Villa and Villa) (Coleoptera: Bostrichidae) was increasingly attracted. Due to their relatively short-range (<0.5 m) inhibition of attractive sources, piperitone, and verbenone dispensers should be placed on avocado trunks where PSHB aggregations occur before the flight season.
Effect of light stress from phytoplankton on the relationship between aquatic vegetation and the propagule bank in shallow lakes

SUMMARY 1. The way light stress controls the recruitment of aquatic plants (phanerogams and charophytes) is a key process controlling plant biodiversity, although still poorly understood. Our aim was to investigate how light stress induced by phytoplankton, that is, independent from the aquatic plants themselves, determines the recruitment and establishment of plant species from the propagule bank. The hypotheses were that an increase in light stress (i) decreases abundance and species richness both of established aquatic plants and of propagules in the bank and (ii) decreases the recruitment success of plants from this bank. 2. These hypotheses were tested in 25 shallow lakes representing a light stress gradient, by sampling propagule banks before the recruitment phase and when the lakes are devoid of actively growing plants (i.e. at the end of winter), established vegetation at the beginning of the summer and phytoplankton biomass (chlorophyll a) during the recruitment and establishment phase. 3. The phytoplankton biomass was negatively correlated with the richness and abundance of established vegetation but was not correlated with the propagule bank (neither species richness nor propagule abundance). The similarity between the propagule bank and established vegetation decreased significantly with increasing phytoplankton biomass. 4. The contrast in species composition between the vegetation and the propagule bank at the highest light stress suggests poor recruitment from the propagule bank but prompts questions about its origin. It could result from dispersal of propagules from neighbouring systems. Propagules could also originate from a persistent propagule bank formerly produced in the lake, suggesting strong year-to-year variation in light stress and, as a consequence, in recruitment and reproductive success of plants.
Brain functional connectivity upon awakening from sleep predicts inter-individual differences in dream recall frequency.

Why do some individuals recall dreams every day while others hardly ever recall one? We hypothesized that sleep inertia - the transient period following awakening associated with brain and cognitive alterations - could be a key mechanism to explain inter-individual differences in dream recall at awakening. To test this hypothesis, we measured the brain functional connectivity (combined EEG-fMRI) and cognition (memory and mental calculation) of high dream recallers (HR, n=20) and low dream recallers (LR, n=18) in the minutes following awakening from an early-afternoon nap. Resting-state scans were acquired just after or before a 2 min mental calculation task, before the nap, 5 min after awakening from the nap, and 25 min after awakening. A comic was presented to the participants before the nap with no explicit instructions to memorize it. Dream(s) and comic recall were collected after the first post-awakening scan. As expected, between-group contrasts of the functional connectivity at 5 min post-awakening revealed a pattern of enhanced connectivity in HR within the default mode network (DMN) and between regions of the DMN and regions involved in memory processes. At the behavioral level, a between-group difference was observed in dream recall, but not comic recall. Our results provide first evidence that brain functional connectivity right after awakening is associated with inter-individual trait differences in dream recall, and suggest that the brain connectivity of HR at awakening facilitates the maintenance of the short-term memory of the dream during the sleep-wake transition.
Performance of CVD risk equations for older patients assessed in general practice: a cohort study.

AIMS To investigate how well the New Zealand PREDICT-CVD risk equations, derived in people aged 30-74 years and US Pooled Cohort Equations (PCEs) derived in people aged 40-79 years, perform for older people. METHODS The PREDICT cohort study automatically recruits participants when clinicians use PREDICT software to conduct a CVD risk assessment. We identified patients aged 70 years and over, without prior CVD, renal disease or heart failure who had been risk assessed between 2004 and 2016. Equation performance was assessed in five-year age bands using calibration graphs and standard discrimination metrics. RESULTS 40,161 patients (median 73 years; IQR 71-77) experienced 5,948 CVD events during 185,150 person-years follow-up. PREDICT-CVD equations were well calibrated in 70-74 year olds but underestimated events for women from 75 years and men from 80 years. Discrimination metrics were also poor for these age groups. Recalibrated PCEs overestimated CVD risk in both sexes and had poor discrimination from age 70 years for men and from age 75 years for women. CONCLUSIONS While PREDICT-CVD equations performed better than PCEs, neither performed well. Multimorbidity and competing risks are likely to contribute to the poor performance and new CVD risk equations need to include these factors.
Increased concentrations of bioactive adrenomedullin subsequently to angiotensin‐receptor/neprilysin‐inhibitor treatment in chronic systolic heart failure

The clinically investigated rationale for neprilysin (NEP)‐inhibition by angiotensinreceptor‐NEPinhibitor (ARNi) therapy is to induce elevations in endogenous natriuretic peptides. NEP, however, cleaves a broad spectrum of substrates, which partially hold significant implications in heart failure with reduced ejection fraction (HFrEF). The effect of NEP inhibition on these peptides has not been investigated thoroughly. This study explored the response of adrenomedullin (ADM) regulation to the initiation of ARNi.
Citric acid excretion and precipitation of calcium citrate in the rhizosphere of white lupin (Lupinus albus L.)

Abstract. White lupin (Lupinus albus L.) was grown for 13 weeks in a phosphorus (P) deficient calcareous soil (20% CaCO3, pH(H2O)7.5) which had been sterilized prior to planting and fertilized with nitrate as source of nitrogen. In response to P deficiency, proteoid roots developed which accounted for about 50% of the root dry weight. In the rhizosphere soil of the proteoid root zones, the pH dropped to 4.8 and abundant white precipitates became visible. X-ray spectroscopy and chemical analysis showed that these precipitates consisted of calcium citrate. The amount of citrate released as root exudate by 13-week-old plants was about 1 g plant−1, representing about 23% of the total plant dry weight at harvest. In the rhizosphere soil of the proteoid root zones the concentrations of available P decreased and of available Fe, Mn and Zn increased. The strong acidification of the rhizosphere and the cation/anion uptake ratio of the plants strongly suggests that proteoid roots of white lupin excrete citric acid, rather than citrate, into the rhizosphere leading to intensive chemical extraction of a limited soil volume. In a calcareous soil, citric acid excretion leads to dissolution of CaCO3 and precipitation of calcium citrate in the zone of proteoid roots.
Immunohistochemical Localization of Thymosin b4 in Bovine Tissues Ourania E. TSITSILONIS 1

The histochemical localization of thymosin b4 was investigated for the first time in several bovine tissues, using the indirect immunofluorescent technique and the newly applied alkaline phosphatase anti-alkaline phosphatase (APAAP) antibody method. A polyclonal antiserum raised against the N-terminal fragment (1-14) of thymosin b4, showing less than 0.1% crossreactivity with the second b-thymosin in calf tissues, thymosin b9, was used. Positive reaction for thymosin b4 was observed in the cytoplasm of all tissues studied, i.e., bovine spleen, thymus, lung, liver, kidney, lymph nodes and cardiac muscle. The highest staining intensity was found in the cytoplasmic area of spleen and thymus, whereas in sections of muscle the reaction was very weak. In the latter case a relatively strong reaction was strictly located in the sarcolemn region of the myofibrils.
Foraging Site Selection of Two Subspecies of Bar-tailed Godwit Limosa lapponica: Time Minimizers Accept Greater Predation Danger Than Energy Minimizers

Different spatial distributions of food abundance and predators may urge birds to make a trade-off between food intake and danger. Such a trade-off might be solved in different ways in migrant birds that either follow a time-minimizing or energy-minimizing strategy; these strategies have been assigned to two sub-species of Bar-tailed Godwits Limosa lapponica that use the European Wadden Sea during northward migration. At the study area on Terschelling we recorded feeding site selection, time budgets and intake rates (prey/min) in the period that both lapponica (energy minimizer) and taymyrensis (time minimizer) sub-species were present (late April till the end of May 2007). Prey availability (number of prey/m2) was negatively correlated to the distance from cover. Based on resightings of colour-ringed Bar-tailed Godwits, taymyrensis was foraging closer to cover, and for a higher proportion of time than lapponica (67% vs. 33%). During the high tide period taymyrensis was also foraging on inland coastal meadows, Moreover, taymyrensis was more vigilant than lapponica, whereas lapponica showed more resting and preening behaviour. Lapponica had a higher instantaneous intake rate, but taymyrensis had a higher overall intake rate and the birds were more successful in taking larger prey items than lapponica. Supposedly, due to the increased foraging time and additional foraging on the inland meadows, the time-minimizing taymyrensis achieved a higher fuel deposition rate than lapponica. Taymyrensis shifted towards food-rich areas, apparently accepting higher predation risks, whereas energy-minimizing lapponica avoided predation danger by foraging further from cover.
FINE STRUCTURE OF ZOOSPOROGENESIS, ZOOSPORE GERMINATION, AND EARLY GAMETOPHYTE DEVELOPMENT IN CLADOPHORA SURERA (CLADOPHORALES, CHLOROPHYTA)

The fine structure of zoosporogenesis, zoospore germination, and early gametophyte development in Cladophora surera Parodi et Cáceres were studied. Zoosporogenesis started with simultaneous meiosis in all nuclei of apical initial cells. The resulting haploid nuclei duplicated in turn by successive centric, closed mitoses. Then, each initial cell divided into two short zoosporangia. Numerous vacuoles appeared around each sporic nucleus. The delimitation of uninucleate zoosporocytes occurred by cytokinetic furrows produced by the coalescence of tiny, clear vesicles, without microtubules. Final shape of the zoospore resulted from gradual expulsion of vacuoles from the cell body. Mature biflagellate zoospores exhibited a conspicuous apical papilla containing fine granular globules, the basal apparatus, and a microtubular “umbrella” formed by numerous cortical microtubules that ran backward the length of the cell body. The chloroplast showed a conspicuous eyespot. The zoosporangial wall disorganized at the pore through which the zoospores were liberated. Zoospores settled on a substrate by their anterior papilla secreting an adhesive. Germination involved retraction of the apical papilla, loss of the “umbrella” microtubules and eyespot, and the lateral absorption of the entire flagellar apparatus, i.e. basal apparatus plus axoneme, into the cytoplasm. Early gametophyte development involved the synthesis of a thin, young cell wall, the development of outer peripheral vacuoles, the appearance of the marginal reticulate chloroplast, and the formation of the first central vacuoles derived from abundant endoplasmic reticulum. Close to the plasmalemma ran longitudinally oriented cortical microtubules. Eventually, the germling developed an achlorophylic, elongated rhizoidal portion.
PP2A:B56ϵ is required for Wnt/β-catenin signaling during embryonic development

The Wnt/β-catenin pathway plays important roles during embryonic development and growth control. The B56 regulatory subunit of protein phosphatase 2A (PP2A) has been implicated as a regulator of this pathway. However, this has not been investigated by loss-of-function analyses. Here we report loss-of-function analysis of PP2A:B56ϵ during early Xenopus embryogenesis. We provide direct evidence that PP2A:B56ϵ is required for Wnt/β-catenin signaling upstream of Dishevelled and downstream of the Wnt ligand. We show that maternal PP2A:B56ϵ function is required for dorsal development, and PP2A:B56ϵ function is required later for the expression of the Wnt target gene engrailed, for subsequent midbrain-hindbrain boundary formation, and for closure of the neural tube. These data demonstrate a positive role for PP2A:B56ϵ in the Wnt pathway.
The invasive Ponto-Caspian mysid, Hemimysis anomala, reaches the UK

The invasive Ponto-Caspian mysid crustacean Hemimysis anomala has been recorded for the first time in the UK in November 2004, and in 2005 at a number of sites in the English Midlands. It occurs in both lentic and lotic environments, including the margins of the relatively fast-flowing River Trent, and large swarms have been observed in one lake. It is not known how the mysid reached the English Midlands, although one site hosts international rowing events.
INTERACTIONS BETWEEN UV‐B EXPOSURE AND PHOSPHORUS NUTRITION. II. EFFECTS ON RATES OF DAMAGE AND REPAIR 1

The interactive effects of P starvation and exposure to UV radiation (UVR) on rates of damage (k) and repair (r), modeled from exposure response curves (ERCs), in the chlorophyte microalga Dunaliella tertiolecta Butcher were investigated. When nutrient‐replete cells were exposed to the UVR during growth, k and r both increased by approximately 62% and 100%, respectively. However, when cells were starved of phosphorus, k increased by a similar amount as observed in replete cells, but r decreased by about 70%, explaining the increased susceptibility of cells to UVR‐induced inhibition of photosynthesis under P starvation. Although not specifically investigated in this study, it is argued that the decreased repair capacity under P starvation is due to a decline in nucleotides such as ATP and GTP, which are necessary for protein repair.
Effects of preceding maize (Zea mays) and cowpea (Vigna unguiculata) in sole cropping and intercropping on growth, yield and nitrogen requirement of okra (Abelmoschus esculentus)

Field experiments were conducted on Alfisols in Nigeria between 1991 and 1993 to determine the residual effect of maize (Zea mays) and cowpea (Vigna unguiculata) on the growth, pod yield and N response of a succeeding okra (Abelmoschus esculentus) crop. Cowpea and maize were grown alone with 30 and 60 kg/ha of N, P and K, respectively, or intercropped with 60 kg/ha as a basal dressing. The preceding crops had a significant effect on soil nutrient changes, okra growth and yield, and N response of okra. Cowpea increased the N, P and K status of the soil in both sole and mixed stands, compared with sole maize. Leaf area, pod weight and marketable pod yield of okra after sole cowpea or the maize/cowpea intercrop were all significantly higher than after sole maize. Although the application of nitrogen to succeeding okra promoted growth and increased pod yield, this was not accompanied by an increase in the pod yield of okra after sole cowpea or the maize/cowpea treatment beyond 45 kg N/ha. The beneficial effects of the preceding maize/cowpea intercrop on soil fertility, okra pod yield and amounts of N required for okra were partly due to the higher rate of basal nutrients applied as compared to the effects following sole cowpea.
Comparison of the Fatty Acid Profiles of the Meat of Crossbreds with 75% Charolais Blood Proportion and Thai Indigenous Upland Cattle

The objective of this study was to compare the fatty acid composition of the Longissimus dorsi muscle of Charolais crossbreds (75%; with 25% Thai native cattle blood) and Thai indigenous Upland cattle (n=8 each). The animals were fed ad libitum grass and supplemented with concentrate at 1.5%/day of body weight. The cattle were on average four years old at slaughter. As a result, the crossbred beef had higher proportions of C16:0 and C18:0 of total lipids than the Upland cattle (P<0.05). At the same time, C14:1 and C18:2 cis-9, trans-11 (conjugated linoleic acid) was lower than in Upland cattle (P<0.05). Overall, this meant higher saturated fatty acid proportions in the crossbreds compared with the Upland cattle, while the opposite was true for the sums of monounsaturated fatty acids and polyunsaturated fatty acids, and the ratio of polyunsaturated to saturated fatty acids (P<0.05). This meant that the beef from the Upland cattle was better nutritionally for humans than the crossbreds.
Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeast Schizosaccharomyces pombe

ABSTRACT Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission yeast Schizosaccharomyces pombe. We compared relative levels of proteins extracted from cells harvested around meiosis I with those of meiosis II, and proteins from premeiotic S phase with the interval between meiotic divisions, when S phase is absent. Our proteome datasets revealed peptides corresponding to short open reading frames (sORFs) that have been previously identified by ribosome profiling as new translated regions. We verified expression of selected sORFs by Western blotting and analyzed the phenotype of deletion mutants. Our data provide a resource for studying meiosis that may help understand differences between meiosis I and meiosis II and how S phase is suppressed between the two meiotic divisions.
Types and distribution of mucous cells of the abalone Haliotis diversicolor

The types and distribution of mucous cells of Haliotis diversicolor were observed and analyzed using the alcian blue and periodic acid schiffs (AB-PAS) reaction and histological procedures. According to the color of the mucous cells, they were divided into four types: Type I, pure red; type II, pure blue; type III, purple reddish; type IV, blue purple. Most of the mucous cells in the mantle were type II, with cup- or stick-like shape. Gill axis and gill filament epithelia were rich in mucous cells and most of them were type II and III, with circle-, cup- and stick-like shapes. There were a few mucous cells in the pedal epithelia, mainly type II, whereas, the pedal gland had a great density of mucous cells, which were large or small and mainly type II and IV. There were many mucous cells in the epithelia around the mouth, most of which were medium-sized cup-shaped type II. In the esophagus, the number of mucous cells decreased gradually from the anterior to posterior, whereas in the distal intestine mucous cells, there were more than in the proximal one and they were more cup–shaped and circle–shaped, mainly type II. Keywords: Haliotis diversicolor ; mucous cells, types, distribution
The Usefulness of Esophageal Baseline Impedance Levels for the Diagnosis of Nonerosive Reflux Disease and the Proper Time for Measurement in Endoscopy-negative Korean Patients With Esophageal or Supraesophageal Symptoms

Background/Aims Low baseline impedance levels (BILs) have been suggested to be evidence of GERD. The aim of this study is to investigate the usefulness of esophageal BILs for the diagnosis of nonerosive reflux disease (NERD) and the proper time for measurement in endoscopy-negative Korean patients with esophageal or supraesophageal symptoms. Methods Endoscopy-negative patients with esophageal or supraesophageal symptoms who underwent esophageal multichannel intraluminal impedance-pH monitoring were included. BILs were measured in the proximal and distal esophagus around 10 minutes before meals, 10 minutes and 30 minutes after meals, 30 minutes before the start of nighttime sleep, and 30 minutes and 60 minutes after the start of nighttime sleep. Results A total of 104 patients were included in the study. Distal and proximal esophageal BILs were decreased after meal ingestion. The BILs of the distal esophagus were significantly lower at all time points in the NERD group, but not in the reflux hypersensitivity (RH) group, compared with the functional group. The area under the receiver operating characteristic curve for the diagnosis of NERD was significant at all time points, but that for the diagnosis of RH was not. The cut-off value of 2375 Ω or 2125 Ω measured around 30 minutes before or 60 minutes after the start of nighttime sleep, respectively, were appropriate for the diagnosis of NERD. Conclusion The BILs of the distal esophagus measured at time points before or after the start of nighttime sleep appear to be useful for the diagnosis of NERD, but not for the diagnosis of RH, in endoscopy-negative Korean patients with esophageal or supraesophageal symptoms.
Effect of seed different levels of NPK fertilizers and irrigation on yield and nutritive quality of mulberry leaf

Experiment was conducted at the research field of Bangladesh Sericulture Research and Training Institute, Rajshahi in 2001-2002 and 2002-2003 to investigate the effect of NPK fertilizers and frequency of irrigation on leaf yield, yield components and leaf quality characters of three mulberry varieties. Four levels of NPK fertilizers and three levels of irrigation were adopted. The treatments comprising 300 kg N, 150 kg P, and 100 kg K/ha/yr along with two irrigations in a month was found to be the best for higher leaf yield, leaf moisture and leaf nutrient contents of mulberry plants (var. BM-3). Key Words: Mulberry, NPK fertilizers, irrigation, nutritive quality. DOI: 10.3329/bjar.v34i3. 3969 Bangladesh J. Agril. Res. 34(3) : 435-442, September 2009