Miracle-dz/UniFAR-FTUs huggingface dataset:
Fine-Tuning of mTOR mRNA and Nucleolin Complexes by SMN

Increasing evidence points to the Survival Motor Neuron (SMN) protein as a key determinant of translation pathway. Besides its role in RNA processing and sorting, several works support a critical implication of SMN in ribosome biogenesis. We previously showed that SMN binds ribosomal proteins (RPs) as well as their encoding transcripts, ensuring an appropriate level of locally synthesized RPs. SMN impacts the translation machinery in both neural and non-neural cells, in agreement with the concept that SMN is an essential protein in all cell types. Here, we further assessed the relationship between SMN and translation-related factors in immortalized human fibroblasts. We focused on SMN-nucleolin interaction, keeping in mind that nucleolin is an RNA-binding protein, highly abundant within the nucleolus, that exhibits a central role in ribosomes production. Nucleolin may also affects translation network by binding the mammalian target of rapamycin (mTOR) mRNA and promoting its local synthesis. In this regard, for the first time we provided evidence that SMN protein itself associates with mTOR transcript. Collectively, we found that: (1) SMN coexists with nucleolin–mTOR mRNA complexes at subcellular level; (2) SMN deficiency impairs nucleolar compartmentalization of nucleolin, and (3) this event correlates with the nuclear retention of mTOR mRNA. These findings suggest that SMN may regulate not only structural components of translation machinery, but also their upstream regulating factors.
Análise multivariada integrada para avaliação dos efeitos do manejo pré-abate na qualidade da carne suína

The aim of this study was to investigate the effect of pre-slaughter handling on the occurrence of PSE (Pale, Soft, and Exudative) meat in swine slaughtered at a commercial slaughterhouse located in the metropolitan region of Dourados, Mato Grosso do Sul, Brazil. Based on the database (n=1,832 carcasses), it was possible to apply the integrated multivariate analysis for the purpose of identifying, among the selected variables, those of greatest relevance to this study. Results of the Principal Component Analysis showed that the first five components explained 89.28% of total variance. In the Factor Analysis, the first factor represented the thermal stress and fatiguing conditions for swine during pre-slaughter handling. In general, this study indicated the importance of the pre-slaughter handling stages, evidencing those of greatest stress and threat to animal welfare and pork quality, which are transport time, resting period, lairage time before unloading, unloading time, and ambience.
Optimization by simulation in manufacturing flow control

A class of piecewise-deterministic control problems typically associated with manufacturing flow-control models is considered. The authors consider different approaches for the definition of a sub-optimal control policy. In particular, the method consisting of optimizing a policy in a class indexed over a given parameter set is addressed. This approach transforms a stochastic control problem into a parameter design problem. It is shown how these models can be used to represent preventive maintenance and/or setup costs in manufacturing flow-control models. The possibility of using an optimization-by-simulation approach to solve the policy-design problem is discussed. <<ETX>>
Hydrodynamic Coefficients for Wellhead Structures

This Master of Science Thesis is written at the Norwegian University of Science and Technology (NTNU), in Trondheim, Norway. The M.Sc. Thesis is executed during the spring of 2011. This thesis is a result of a request from FMC Technologies, Kongsberg Subsea to do research of the hydrodynamic coefficients drag and added mass. The purpose of this thesis is to find hydrodynamic coefficients for added mass and drag for a XmasTree (XT) by physical model tests and numerical calculations. A XT is a a subsea oil well valve tree, which is put on top of a well head, and used for opening and closing the oil flow from the well. During a XT life, it experience most loads during lowering through the wave zone, as can be seen in figure 1. The XT is a complex structure, and calculation of hydrodynamic coefficients for these structures are not straight forward. Coefficients can be found by finding coefficients for each member of the structure. This method is quite uncertain, and would be inaccurate in this case due to the many members. Because of the complexity of the structure a Computional Fluid Dynamics (CFD) analysis or other numerical analysis will presumably take months. A model test is therefore the best alternative for finding these coefficients. Three different models are built to see the difference between them during these tests. One of the models is a solid box, that can be compared with theory of hydrodynamic coefficients of this sort of structure. The two other models are more similar to the XT, and have differences requested by FMC Technologies, Kongsberg Subsea (FMC). The measurements achieved during these test are scaled up to full scale values. This is possible since our models are valid as Froude models. The results are presented and compared for each parameter fixed. In addition to the physical model tests, a numerical calculation is carried out by WAMIT. WAMIT is a computer program that is based on potential theory, and is used for analyzing floating and submerged bodies subjected to ocean waves. From this calculation we will get added mass of the models. The calculated drag and added mass coefficient from the model tests and the numerical calulation is to be compared with theoretical hydrodynamic coefficients of this structure.
Iron Status in Preschool Children with Normal Hemoglobin, Egypt

BACKGROUND: Iron deficiency (ID) anemia remains a common cause of anemia in young children. However, the more significant than anemia itself is the ID without anemia that also adversely affect neurocognitive development and unfortunately some of these effects may be irreversible. AIM: This study is a cross-sectional study aimed to asses iron status in preschool children with normal hemoglobin (Hgb) level attending Assiut University Children Hospital. METHODS: The cross-sectional study including 68 apparently healthy children aged 1–6 years old during the period from January 1, 2015 to June 30, 2015, They were subjected to detailed history, physical examination, and the following laboratory investigations: Complete blood count, Serum iron, Total Iron Binding Capacity, Serum ferritin, and Transferrin saturation. RESULTS: Low serum ferritin and low transferrin saturation were detected in 41.2% and 47% respectively of our studied children who have normal Hgb levels. CONCLUSION: Normal Hgb doesn’t exclude ID that should be screened in healthy children to prevent the possible long-term effects of ID on their cognation and mental development.
Burnout of undergraduate construction management students in Australia

Burnout has been identified as a serious problem for the Australian construction industry, having negative effects at both individual and the organizational levels. While there is accumulating research into the causes and consequences of burnout for professionals working in the construction industry, we have little understanding of construction students’ susceptibility to burnout. This is despite constantly increasing pressures to self-fund their studies and balance the extra demands of study, on top of work-life demands. Using the Maslach Burnout Inventory – Student Survey (MBI-SS), a survey of 369 construction management students from across eight Australian universities shows that Australian students suffer from considerably higher levels of burnout than comparative professional samples. Levels of burnout showed a relationship with both their time spent at work and their overall satisfaction with study and work life, raising important issues for both educators and employers. It was also found that academic study may not be the primary life domain of many of these students and that theoretically, the notion of work-life-study balance may be a more appropriate way, than traditional concepts of work-life balance, to conceptualize the challenges faced by these students.
HUMAN FACTORS DESIGN GUIDELINES FOR MULTIFUNCTION DISPLAYS

Abstract : References related to the design of multifunction displays are summarized into a series of human factors design guidelines. These are presented in a number of sections, which include: general guidelines, the design process, air traffic displays, weather displays, navigation displays, MFD menu organization, automation, individual displays, and general design principles for aircraft displays. Particularly relevant data graphs are included to illustrate some of the recommendations. These sections are followed by samples from the extant body of guidelines and standards.
Nicotinic modulation insecticides act on diverse receptor subtypes with distinct subunit compositions

Insect nicotinic acetylcholine receptors (nAChRs) are ligand gated ion channels mainly expressed in the central nervous system of insects. They are the directed targets of nicotinic modulation insecticides including neonicotinoids, the most widely used insecticides in the world. However, the resistance development from pests and the negative impacts on the pollinators affect their applications and create demand for the alternatives. Thus, it is very important to understand the mode of action of these insecticides at the molecular level, which is actually unclear for more than 30 years. In this study, we systematically examined the susceptibility of ten Drosophila melanogaster nAChR subunits mutants against eleven nicotinic modulation insecticides. Our results showed that there are several subtypes of nAChRs with distinct subunits compositions that are responsible for the toxicity of different insecticides, respectively. At least three of them are the major molecular targets of seven structurally similar neonicotinoids in vivo. On the other hand, the spinosyns may exclusively act on the α6 homomeric nAChR but not any other heteromeric pentamers. Behavioral assays using thermogenetic tools further confirmed the bioassay results and support the idea that receptor activation rather than inhibition leads to the insecticidal effects of neonicotinoids. The present findings reveal native nAChR subunit interactions with various insecticides and have important implications for resistance management and the development of novel insecticides targeting this important ion channels. Author Summary The neonicotinoids and spinosyns make up about 27% of the insecticides by world market value. Novel insecticides like sulfoxaflor, flupyradifurone and triflumezopyrim are developed as alternatives due to the negative effects of neonicotinoids on pollinators. Although all act via insect nicotinic acetylcholine receptors, the mode of action is unclear. Our work shows that these insecticides act on diverse receptor subtypes with distinct subunit compositions. This finding could lead to the development of more selective insecticides to control pests with minimal effects on beneficial insects.
Inputs of Total and Labile Dissolved Metals from Six Facilities Continuously Discharging Treated Wastewaters to the Marine Environment of Gran Canaria Island (Canary Islands, Spain)

The presence of ten metals (Cd, Ni, Pb, Cr, Cu, Zn, Al, Fe, Mn, and Co) was investigated in the final discharge of six facilities, including four wastewater treatment plants, which were continuously discharging treated wastewater to the coastal environment in Gran Canaria Island. A four-day sampling campaign was carried out at each facility in July 2020, in which both the spot samplings technique and the diffusive gradient in thin-film technique (DGT) were carried out to measure total dissolved metals and the in situ labile metal fraction, respectively. After the necessary sample preparation steps, measurements were carried out by ICP-MS for both samplings. Raw data referred to the spot total dissolved and DGT-labile metal concentrations were reported. In general, the average metal concentrations were dispersed in a broad range. As expected, the highest metal contents were found in those facilities with larger industrial contributions. The values of annual average environmental quality standards (AA-EQS) were used to assess the total dissolved metal concentrations for every metal in every final discharge. In only one of the studied facilities, some metals (Ni and Zn) exceeded these EQS within the receiving waterbody, highlighting the need for more efficient treatment targeted towards a specific discharging-water quality. In addition, the total dissolved and labile metal daily fluxes of discharge were calculated to estimate the contribution of every effluent to the receiving water bodies.
Determinants of Consumers’ Trust in Biotech Brands and Purchase Intentions towards the Cord Blood Products

The development of the biotech industry is in full swing, and consumers have begun to value biotech brands. Since biotech products often focus on the future or special benefits, consumers inevitably bear certain risks when purchasing biotech products, and their trust in the biotech brand will have an important impact on their purchase intention. Previous studies have lacked a targeted understanding of consumer trust in biotech brands and a discussion of cultural viewpoints. This study introduced the concept of personal connections in Chinese relationalism and trust strategies in Chinese society to address this gap. In-depth interviews and focus group discussions were conducted in collaboration with Company X, a listed Taiwanese cord blood company, to extract the key factors influencing consumer trust and purchase intention of biotech brands. After constructing the structure model, the study was validated using a structural equation model through investigation and survey. The findings indicated that consumer trust in biotech brands was constructed by a combination of kinship trust transfer and emergent trust transfer within the consumer relationship network, as well as institutional trust and professional trust outside the relationship network and that a significant positive correlation existed between consumer trust in biotech brands and purchase intention. The acquaintances within the consumer relationship network include not only relatives and friends but also health care workers and netizens that consumers come into contact with. In addition, kinship trust transfer and emergent trust transfer within the consumer relationship network have a greater impact on trust in biotech brands than the institutional trust and professional trust outside the relationship network. The findings of this study deepen the understanding of consumer trust in biotech brands across cultures, and suggest that future marketing communication should be expanded to include key players within the consumer relationship network.
Wound-like tumor periphery in human breast cancer predicts a convergent drug nonresponse

A significant portion of breast cancer patients are nonresponsive to well-established drugs and destined for a poor outcome regardless of molecular subtype. Although several (multiparameter) molecular markers have predicted their resistance to some of these drugs, profound uniparameter markers predictive of a convergent nonresponse to all these drugs remain elusive. We employ co-registered standard-multiphoton histology to representatively sample a few peripheral niches of the primary tumor, so that hundreds of patients can be stratified with either a wound-like or non-wound tumor periphery. With no fitting variable, this simple uniparameter morphological marker is: (a) highly sensitive and specific to predict a multidrug-nonresponsive phenotype that accounts for the majority of recurrence or death, independent of the molecular subtype or related adjuvant drug selection, clinical endpoint (disease-free versus overall survival), and hosting medical center; (b) robust against intratumor heterogeneity and valid at the earliest clinicopathological stage; and (c) dominant in predicting prognosis in the context of routine clinicopathological markers. Considering the mechanistic link between a wound-like extracellular matrix and a microenvironment supporting migratory or mesenchymal tumor cells, we attribute these unusual capabilities to an epithelial-mesenchymal transition nature of the morphological marker long sought after by pathologists.
Management of infertile women with mosaic Turner syndrome by using homologous oocyte

Introduction: The aim of the study was to investigate the effect of the use of homologous intracytoplasmic sperm injection on clinical pregnancy and live birth rates in infertile patients with mosaic Turner syndrome (TS). Material and methods: Twelve infertile cases who had unsuccessful in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) trials and were diagnosed with mosaic TS were included in the study. Twelve cases with unexplained infertility were selected as the control group. The diagnosis of TS was made according to the TS guideline. Karyotype identification was made by fluorescence in situ hybridization method (FISH) analysis of peripheral blood samples. The patients whose first cell line consisted of 45,XO and second cell line consisted of any karyotype were accepted as mosaic TS. TS cases with gonadal dysgenesis or classical TS were not included in the study. Homologous intracytoplasmic sperm injection was performed in both groups of patients. While embryo transfer was performed after pre-implantation genetic diagnosis (PGD) in 5 patients, embryos were transferred without PGD in 7 patients. Clinical pregnancy and live birth rates of the patients in both groups were compared. Results: Age and body mass index values of both groups were found to be similar. The number of unsuccessful IVF attempts and duration of infertility were similar in both groups. However, the total dose of recombinant follicle stimulating hormone (rFSH) was higher and the duration of rFSH use was longer in TS patients. The total number of oocytes collected and metaphase II stage (MII) oocytes were significantly lower in the TS group. The 45,X/46,XX karyotypes detected in 6 of 12 cases were recorded as the most frequent mosaic TS pattern. The mosaic form we detected as the second most frequent was 45,X/46,XX/47,XXX karyotype seen in 3 cases. Approximately half of the cases had 45,X/46,XX karyotype, while one fourth had 45,X/46,XX/47,XXX. The remaining 3 cases were the rarer mosaic TS forms. There was no significant difference between the mosaic TS group and the control group in terms of clinical pregnancy rates (58.3% vs. 50%, p < 0.55). Live birth rates were found to be significantly higher in the control group compared to the TS group (57.1% vs. 83.3%, p < 0.001). Abortion rates were 42.8% in the TS group and 16.6% in the control group. The abortion rate in the TS group was approximately 2.6 times higher than in the control group (p < 0.02). One of the three miscarriages was from the PGD group (33.3%), and the remaining two were from the non-PGD group (66.6%). Conclusions: Before an infertile patient with mosaic TS is referred for donation, IVF/embryo transfer (ET) should be planned with her own eggs and by applying PGD.
History-taking, Clinical Signs, Tests and Scores for Detection of Non-organic Dyspepsia (NOD) Among Patients With Acute Abdominal Pain (AAP).

Background/Aim The diagnostic accuracy of history-taking, clinical signs and tests and diagnostic scores (DSs) for patients with non-organic dyspepsia (NOD) have been rarely evaluated. Patients and Methods A cohort of 1333 patients presenting with acute abdominal pain (AAP) were studied, including 50 patients with confirmed NOD. The most significant diagnostic variables (in multivariate logistic regression analysis) were used to construct six different DS models and their diagnostic accuracy was compared with clinical symptoms and signs and tests. Meta-analytical techniques were used to detect the summary sensitivity (Se) and specificity (Sp) estimates for each data set (symptoms, signs and tests as well as DS models). Results In hierarchical summary receiver operating characteristic (HSROC) analysis, the area under curve (AUC) values for i) symptoms ii) signs and tests iii) DS were as follows: i) AUC=0.608 [95% confidence interval (CI)=0.550-0.666]; ii) AUC=0.621 (95% CI=0.570-0.672) and iii) AUC=0.877 (95% CI=0.835-0.919). The differences between these AUC values (roccomp analysis) are as follows: between i) and ii) p=0.715; between i) and iii) p<0.0001; between ii) and iii) p<0.0001. Conclusion The present study is the first to provide evidence that the DS could be used in diagnosis of NOD. The major advantage of our DS is that this model does not need radiology or endoscopy to reach high diagnostic accuracy.
Comparison of efficacy of extracorporeal magnetic innervation and Kegel exercises for stress urinary incontinence in adult women: study protocol for a randomized controlled trial

Stress urinary incontinence (SUI) is defined as a complaint of inadvertent loss of urine occurring as a result of an increase in intraabdominal pressure. Strong evidence supports the use of pelvic floor muscle training (PFMT) as the first-line conservative treatment for SUI. Extracorporeal magnetic stimulation (EMS) is a non-invasive, effective, acceptable, and safe therapeutic modality for SUI. Although PFMT and EMS share most of their influences on the pathophysiology of SUI, it is unclear whether one of these routinely used treatment modalities is superior to another in terms of improvement of clinical outcomes or cost-effectiveness. To the best of our knowledge, no randomized controlled trials have so far directly compared PFMT with EMS. Our aim here is to describe a protocol for such a study. This will be a parallel-group, single-blind, randomised controlled trial compliant with the SPIRIT, CONSORT, and TIDieR reporting guidelines. Participants will be women aged 18 to 65 years who have previously given at least one vaginal delivery (at least 12 months before joining the study) who present with symptoms of SUI lasting at least 6 months yet have not previously received treatment for it. In the first study arm, patients will receive an eight-week, high-intensity, home-based Kegel exercises regimen. In the second study arm, the treatment scheme will consist of 2 sessions of EMS per week for a total of eight weeks. The primary outcome will be effectiveness of treatment as measured by the International Consultation on Incontinence Questionnaire Urinary Incontinence-Short Form overall score, eight weeks, three months, and six months after commencement of treatment.
Speciation of P in Solid Organic Fertilisers from Digestate and Biowaste

Phosphorus (P) is a finite resource and its reuse in organic fertilisers made from biowaste and manure should therefore be encouraged. The composition of solid organic fertilisers (SOFs) depends on the type of feedstock and processing conditions, and this may affect P speciation and hence P availability. Phosphorus speciation was assessed in eighteen different SOFs produced from biowaste and digestate. Available P was determined in 10 mM CaCl2 extracts at a fixed pH of 5.5 and at a fixed total P concentration in the suspension. P was dominantly present as inorganic P (>80% of total P). There was a strong variation in the Fe content of the SOFs and hence in the fraction of P bound to reactive Fe/Al-oxides (PFe). The fraction of total P soluble at pH 5.5 correlated negatively with PFe pointing to fixation of P by metal salts added during processing, or by soil mineral particles in case garden waste was processed. Therefore, the use of iron salts in processing plants should be avoided. In addition, the presence of P in poorly soluble precipitates lowered the fraction of easily available P. Overall, this study shows that Pt alone is not a good indicator for the agronomic efficiency of SOFs due to large differences in P speciation among SOFs.