Miracle-dz/UniFAR-FTUs huggingface dataset:
MICROPROPAGATION OF SIX PRUNUS MUME CULTIVARS THROUGH AXILLARY SHOOT PROLIFERATION, AND ISSR ANALYSIS OF CLONED PLANTS

Prunus mume (Mei flower) is one of the most popular landscape plants in China and Japan. It is well known worldwide for its winter flowering and for the fragrance of its differently colored flowers. Every year, P. mume gardens bring commercial benefits by attracting numerous visitors. To date there are 323 cultivars available, thanks to long-term conventional breeding and improvement of cultivars by breeders (Chen, 1997). To satisfy a growing interest in constructing P. mume gardens for conservation of their diversity of cultivars, and for commercial reasons, there is an urgent need to raise high-quality seedlings of wild and cultivated P. mume varieties. Unfortunately, propagation of P. mume through cuttings or through seeds or root suckers is a slow and laborious process. Moreover, the germination frequency of the seeds is very low, as is the survival rate of grafts. Significant progress has been made in the development of tissue, organ and cell culture in different woody plants (Sahoo and Chand, 1998; Birod et al., 2004; Boggetti et al., 1999). Work on the propagation (Webster, 1980; Muna et al., 1999; Duguma and Bell, 2002) and regeneration (James, 1984; Grant and Hammat, 2000) of Prunus species has also been reported. In recent years, in vitro culture, a set of techniques that offers a tool for mass multiplication, germplasm conservation and transformation research, has been widely used in Prunus species, but very few reports have described micropropagation of P. mume. As in vitro culture is usually associated with somaclonal variation, it is necessary to establish a micropropagation system that produces genetically identical and stable plants, especially in the case of commercial plant species such as P. mume. Although RAPD analyses have usually been performed to evaluate genetic variation, ISSR is more effective and reproducible for quick assessment of the genetic stability of plants (Goto et al., 1998; Martins et al., 2004). To our knowledge, only Harada and Murai (1996) have suggested that it is possible to propagate P. mume through in vitro culture, but that work was limited to a few genotypes and had problems in
Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade.

INTRODUCTION Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. METHODOLOGY A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. RESULTS The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). CONCLUSIONS Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade.
Inhibition of the catalytic subunit of DNA‐dependent protein kinase (DNA‐PKcs) stimulates osteoblastogenesis by potentiating bone morphogenetic protein 2 (BMP2) responses

The catalytic subunit of DNA‐dependent protein kinase (DNA‐PKcs) is a pleiotropic enzyme involved in DNA repair, cell cycle control, and transcription regulation. A potential role for DNA‐PKcs in the regulation of osteoblastogenesis remains to be established. We show that pharmacological inhibition of DNA‐PKcs kinase activity or gene silencing of Prkdc (encoding DNA‐PKcs) in murine osteoblastic MC3T3‐E1 cells and human adipose‐derived mesenchymal stromal cells markedly enhanced osteogenesis and the expression of osteoblast differentiation marker genes. Inhibition of DNA‐PKcs inhibited cell cycle progression and increased osteogenesis by significantly enhancing the bone morphogenetic protein 2 response in osteoblasts and other mesenchymal cell types. Importantly, in vivo pharmacological inhibition of the kinase enhanced bone biomechanical properties. Bones from osteoblast‐specific conditional Prkdc‐knockout mice exhibited a similar phenotype of increased stiffness. In conclusion, DNA‐PKcs negatively regulates osteoblast differentiation, and therefore DNA‐PKcs inhibitors may have therapeutic potential for bone regeneration and metabolic bone diseases.
Emergency C-section, maternal satisfaction and emotion regulation strategies: effects on PTSD and postpartum depression symptoms

ABSTRACT Objective This cross-sectional study aimed to investigate the relationship between a mother’s current emotion regulation strategy (antecedent-focused vs. response-focused), her satisfaction with childbirth, and posttraumatic and/or depressive symptoms after unplanned C-section. Background The mother’s and baby’s health is considered a priority during childbirth. As a result, situations in which an unplanned C-section is required may not allow mothers to express their needs and emotions. This may lead to feelings of dissatisfaction regarding the childbirth experience. Methods Fifty French participants aged 18–35 (M = 27.10; S.D. = 3.99) who had a C-section were recruited on social networking groups and completed four self-report measures online, up to two years after childbirth. These measures assessed emotion regulation strategies currently used, birth satisfaction, postpartum depression symptoms and PTSD symptoms. Results Main results indicate (1) Mothers who use expressive suppression, a response-focused strategy, are less satisfied with childbirth. (2) Emotion regulation, when combined with maternal satisfaction, shows a negative association with posttraumatic stress score and depression score. Conclusion This study provides interesting data for further research. Follow-up studies about emotion regulation, postpartum depression and posttraumatic stress symptoms should be conducted in the future in order to provide clinical recommendations.
Phylogenetic Relationships of the Mangrove Family Avicenniaceae Based on Chloroplast and Nuclear Ribosomal DNA Sequences

Abstract Chloroplast (rbcL, trnL intron, trnL-trnF spacer) and nuclear ribosomal (ITS1, 5.8S, ITS2) DNA sequences were analyzed to identify the closest terrestrial relatives of the mangrove family Avicenniaceae. These plants have been classified within or near Verbenaceae in most synoptic treatments of angiosperms. Surprisingly, Avicenniaceae were placed as part of Acanthaceae s.l. in analyses of all data sets, using both parsimony and maximum likelihood. Within Acanthaceae s.l. , our analyses consistently placed Avicennia as sister to Thunbergioideae but without strong support. Constrained maximum likelihood analyses indicated that alternative placements of Avicennia near the base of Acanthaceae s.l. were not significantly less likely than the sister group relationship with Thunbergioideae. However, placement with Verbenaceae was significantly less likely, as was placement with Pedaliaceae. Morphological evidence is reviewed in this phylogenetic context, and we suggest that articulated nodes and inflorescence structure (including flowers subtended by a bract and two bracteoles) may provide synapomorphies for Avicennia and Acanthaceae s.l. We can identify no clear morphological synapomorphies linking Avicennia to Verbenaceae. Avicennia shares a number of features with each of its putative relatives that are likely to be symplesiomorphic or are of uncertain phylogenetic status. Communicating Editor: Aaron Liston
Treatment of migraine attacks and prevention of migraine: Guidelines by the German Migraine and Headache Society and the German Society of Neurology

In collaboration with some of the leading headache centres in Germany, Switzerland and Austria, we have established new guidelines for the treatment of migraine attacks and the prevention of migraine. A thorough literature research of the last 10 years has been the basis of the current recommendations. At the beginning, we present therapeutic novelties, followed by a summary of all recommendations. After an introduction, we cover topics like drug therapy and practical experience, non-effective medication, migraine prevention, interventional methods, non-medicational and psychological methods for prevention and therapies without proof of efficacy.
Mental health of forced migrants recently granted leave to remain in the United Kingdom

Background: Asylum seekers who are granted leave to remain in the United Kingdom are required to make a rapid transition to housing and welfare benefits. The challenges facing new refugees during this ‘transition period’ can affect their mental health, but this has not been quantified. Aims: To assess the impact of the transition period on new refugees’ mental health in the 12 months after being granted leave to remain in the United Kingdom. Method: A longitudinal survey design was used to measure the mental health of 30 newly recognised refugees at monthly intervals in the first 6 months and again at 1 year after receiving leave to remain in the United Kingdom. There were five outcome measures for symptoms of anxiety, depression, distress, post-traumatic stress disorder (PTSD), post-migration living difficulties (PMLD) and a life events calendar to record key changes in housing and welfare. Results: The results showed that the trajectory of scores across all measures fluctuates, but overall they all improve from baseline to Month 12. Scores for depression and PMLD showed significant improvement at Month 5, and scores for anxiety, depression, distress and PMLD showed significant improvement at Month 12. PTSD scores did not show significant improvement at any month. In months with a high number of stressful life events, participants had worse PMLD and PTSD scores. Conclusion: Overall improvement in mental health could partly be explained by the stability of being granted leave to remain in the United Kingdom, but may also be due to the high level of practical support these participants received. Recommendations are made for those working with clients during the transition period.
Effects of β-Glucan Addition to a Probiotic Containing Yogurt

This study investigated the effects of addition of beta-glucan from 2 different cereal sources (oat and barley) on growth and metabolic activity of Bifidobacterium animalis ssp. lactis (Bb-12) as determined by plating on a selective medium in yogurt during prolonged cold storage. These yogurt batches were compared to unsupplemented and inulin supplemented controls. All batches were also assessed for syneresis. Oat beta-glucan addition resulted in improved probiotic viability and stability comparable to that of inulin. It also enhanced lactic and propionic acid production. The barley beta-glucan addition suppressed proteolytic activity more than that from oat. These improvements were hindered by greater syneresis caused likely by thermodynamic incompatibility. Small amplitude oscillatory measurements of acidified model mixture of beta-glucan/skim milk solids showed formation of casein gel within the beta-glucan network. Binary mixtures of beta-glucan and skim milk solids had apparent pseudoplastic and non-Newtonian behavior governed mainly by beta-glucan contribution. Above critical concentrations, the mixtures underwent phase separation with the lower phase rich in protein. The phase diagram also showed that the addition of beta-glucan may be possible at or below 0.24 w/w%.
Phylogenetic relationship of TJ1 isolate of Banana bunchy top virus from Pakistan by DNA-R sequence analysis

The disease caused by the Banana bunchy top virus (BBTV) is a major problem in banana crops. It spreads either through vegetative propagation of the plant and (or) by means of a vector, Pentalonia nigronervosa. Planting material for banana cultivation was introduced to Sindh (a major banana-growing province of Pakistan) from many countries where BBTV was present. A recent BBTV epidemic in the Sindh region resulted in considerable damage to the banana crop. To determine the probable origin of this epidemic, BBTV was isolated from bananas in Sindh, and the DNA-R of the resulting TJ1 isolate was cloned, sequenced, and subjected to phylogenetic analysis. Analysis with the complete sequence of the DNA-R showed that the TJ1 isolate is a member of the South-Pacific group. Analysis with the coding or the conserved regions of the DNA-R supported the same grouping. The TJ1 isolate shows the highest homology with the Egyptian isolate within this group, followed by the Australian and Indian isolates. All these isolates seem to have evolved from a common ancestor.
A retrospective study of salivary gland diseases in 179 dogs (2010–2018)

Salivary gland diseases are well characterized in human medicine but are uncommonly reported in dogs. Herein we describe the clinical and pathologic features of 179 canine salivary gland biopsy submissions to the Athens Veterinary Diagnostic Laboratory from 2010 to 2018. The mean age of affected dogs was 8.5 y; no sex or breed predisposition was evident. The main reason for sample submission was regional swelling (107 cases; 59.7%). Extraoral (major) salivary glands were affected in 125 cases (69.8%), and oral (minor) salivary glands were affected in 43 cases (24% of cases). The location of the lesion (extraoral or oral) was not specified in 11 cases (6.1%). The diagnoses included nonspecific sialoadenitis (89 cases; 49.7%), normal salivary gland (42 cases; 23.4%), neoplasia (36 cases; 20.1%), salivary gland lipomatosis (7 cases; 3.9%), necrotizing sialometaplasia (4 cases; 2.2%), and traumatic hemorrhage (1 case; 0.5%). Most cases of sialoadenitis (63 cases), neoplasia (23 cases), and lipomatosis (5 cases), as well as all cases of necrotizing sialometaplasia and the only case of traumatic hemorrhage, affected extraoral glands. Most neoplasms (32 cases, 88.8% of the tumors) were epithelial and malignant, followed by round cell tumors (2 cases; 5.5%), a carcinosarcoma (1 case; 2.7%), and a tumor of undetermined histogenesis (1 case; 2.7%).
Determinant Intellectual Capital Disclosure Factors in Integrated Reporting

This research aims to determine the relationship between firm performance, firm industry, size, and intangible asset to intellectual capital disclosure. Sources of research data consist of integrated reports from publicly listed company from several countries for 2012– 16. The analytical technique used is multiple linear regression analysis using SPSS program version 18 by first doing the classical assumption test. This study shows that performance and size have a significant positive effect on intellectual capital disclosure, otherwise industry has a negative effect on intellectual capital disclosure. The intangible has no significant effect on intellectual capital disclosure. Considering that there is still a small amount of research related to integrated reporting, this research contributes to add study material on issues in integrated reporting, especially factors related to intellectual capital disclosure.
Empowering Leadership, Psychological Empowerment and Employees’ Creativity: A gender perspective

Prior research has shown that employees’ creativity can contribute to the organizational effectiveness, innovation, and survival, and as that it represents an important concept to study. Not surprisingly, there has been an increasing interest in understanding factors that promote employees’ creativity, and one of the persistent factors is empowerment. The present study investigates whether empowering leadership and psychological empowerment positively influence employees’ creativity in Bosnia and Herzegovina. In addition, it explores whether there is a moderating effect of psychological empowerment on the relationship between empowering leadership and employees’ creativity. Finally, this study examines whether there is a difference between female and male employees in empowering leadership and psychological empowerment. Field survey data collected from different companies indicates that empowering leadership and psychological empowerment are positively related to employees’ creativity, while psychological empowerment has no moderating effect. Gender differences are present only regarding empowering leadership.
Association of maize streak virus disease and its vectors (Homoptera: Cicadelidae) with soil macronutrients and altitudes in Kenya.

Maize (Zea mays L.) is a staple food for more than 100 million people in Africa. Its production is constraint by maize streak virus (MSV) disease. This study investigated the interrelationships between altitudes and levels of soil macronutrients with MSV disease and its vectors. The influence of nitrogen, phosphorus and potassium levels on MSV and on populations of Cicadulina spp. (Homoptera: Cicadellidae) were investigated at six geographically separated maize producing regions in Kenya. Comparisons of numbers of sampled Cicadulina spp showed that areas at lower than 1550 m above sea level had significantly more leafhoppers than those at higher altitudes (p 40%) and higher than that in Bahari, Muhoroni, Kimilili and Githunguri (< 30%) and it correlated positively with numbers of Cicadulina mbila. A significant positive correlation was detected between potassium with C. mbila. This study shows that soil nutrient levels influence MSV vector (Cicadulina spp) and that this relationship is critical during the design of crop protection packages.
Canopy openings in a primary neotropical lowland forest

Windthrown trees and large broken branches create a mosaic of light gaps in tropical forests. Viewed from above, these canopy openings are easily recognized and can be surveyed with aerial photos. Within canopy openings, many biological activities take place that occur rarely or not at all elsewhere in the forest near the forest floor. This is mainly a result of the greater amount of sunlight reaching the lowest levels of the canopy strata and frequently but ephemerally, the forest floor. This mosaic of forest structure was first recognized by Aubreville (1938). Recent research has focused on forest regeneration within canopy gaps in primary forest (Brokaw 1982, Hartshorn 1980). Canopy openings are critical for regeneration of many canopy tree species (Denslow 1980, Hartshorn 1978, Lang & Knight 1983, Richards 1952, Whitmore 1978). Nutrient availability and soil nutrient storage may be expected to change in canopy openings (Anderson & Swift 1983, Bazzaz 1983, Orians 1982). Large canopy openings provide one of the few rain forest habitats for many pioneer
Influence of Graminicide Formulation on Compatibility with Other Pesticides

Abstract Experiments were conducted from 2003 through 2006 to compare annual grass control by graminicides applied alone or with other pesticides and to determine whether graminicide formulation affected annual grass control and interactions with co-applied pesticides. Formulation and rate had no affect on broadleaf signalgrass or large crabgrass control by clethodim. The efficacy of clethodim in tank mixtures with acifluorfen plus bentazon, bentazon, chlorothalonil, imazapic, pyraclostrobin, or tebuconazole were not affected by clethodim formulation. Broadleaf signalgrass and large crabgrass control by clethodim was slightly reduced by acifluorfen plus bentazon, chlorothalonil, imazapic, and pyraclostrobin, but not by tebuconazole. Chlorothalonil and pyraclostrobin reduced broadleaf signalgrass control with quizalofop-P but did not reduce fall panicum control. Azoxystrobin, propiconazole, and tebuconazole did not affect efficacy of quizalofop-P. Nomenclature: Acifluorfen; azoxystrobin, methyl (E)-[2-[2-6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl]-3-methoxyacrylate; bentazon; chlorothalonil, tetrachloroisophthalonitrile; clethodim; imazapic; propiconazole, 1-[2-(2,4-dichlorophenyl)-4-propyl-1,3-dioxolan-2-yl-methyl]-1H-1,2,4-triazol; pyraclostrobin, carbamic acid, [2-[[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]methoxy-, methyl ester; quizalofop-P; sethoxydim; tebuconazole, α-[2-(4-chlorophenyl)ethyl]-α-(1,1-dimethylethyl)-1H-1,2,4-triazole-1-ethanol; broadleaf signalgrass, Urochloa platyphylla (Nash) R.D. Webster UROPP; fall panicum, Panicum dichotomiflorum Michx. PANDI; large crabgrass, Digitaria sanguinalis (L.) Scop. DIGSA
Life tables of Bactrocera cucurbitae (Diptera: Tephritidae): with an invalidation of the jackknife technique

Life table gives the most comprehensive description on the survival, stage differentiation and reproduction of a population and is thus the most important basis of population ecology and pest management. In this study, we constructed life tables for Bactrocera cucurbitae on cucumber (Cucumis sativus L.) in the laboratory and under simulated field conditions. To assess the variability of the life tables, we carried out two experiments under each treatment. Means, variances and standard errors of life table parameters were estimated for each of the two experiments by using the jackknife technique. At 25°C, the intrinsic rates of increase (r) found for the two experiments were 0.1354 and 0.1002 per day, and the net reproductive rates (R0) were 206.3 and 66.0 offspring, respectively. For cucumbers kept in the field and covered with leaves, the r and R0 for the two experiments were 0.0935 and 0.0909 per day, and 17.5 and 11.4 offspring, respectively. However, if cucumbers were kept in the field but were not covered, the r and R0 for the two experiments were 0.1043 and 0.0904 per day, and 27.7 and 10.1 offspring, respectively. Our results revealed significant variability between the experiments under both laboratory and field conditions; this variability should be taken into consideration in the data collection and application of life tables. However, our mathematical analysis shows that the application of the jackknife technique will result in biologically unrealistic R0,i‐pseudo and consequently overestimation of the variance of R0. According to our analysis, we suggest that the jackknife technique should not be used for the estimation of variability of the net reproductive rate.