Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence du chemin de chargement sur le comportement du polyamide 11 autour de la transition vitreuse

En dessous de la transition vitreuse, pour des vitesses moyennes de deformation, le suivi de la deformation volumique en traction et la non influence de la pression hydrostatique sur les seuils d'ecoulement plastique obtenus pour differents chemins de chargement montrent que le comportement du polyamide 11 est tres proche de celui des metaux. Les mecanismes de creation et de propagation de bandes de cisaillement intervenant dans la deformation de la phase amorphe du pa11 peuvent etre assimiles a ceux de propagation de dislocations dans les metaux. Cependant, le pa11 acquiert un comportement radicalement different de celui des metaux lorsqu'il est soumis a des conditions favorables aux mecanismes diffusionnels de deformation. Ainsi, toujours en dessous de la transition vitreuse mais pour des vitesses lentes de deformation, des phenomenes de relaxation sensibles a la pression hydrostatique vont se produire autour des bandes de cisaillement de la phase amorphe vitreuse et la recouvrance des deformations va etre retardee. Au dessus de la temperature de transition vitreuse, la deformation de la phase amorphe caoutchoutique s'effectue par diffusion sans engendrer aucune variation de volume ni subir aucune influence de la pression hydrostatique. La recouvrance tres longue traduira la nature entropique de cette deformation
An overview of extracellular matrix and its remodeling in the development of cancer and metastasis with a glance at therapeutic approaches

The extracellular matrix (ECM) is an inevitable part of tissues able to provide structural support for cells depending on the purpose of tissues and organs. The dynamic characteristics of ECM let this system fluently interact with the extrinsic triggers and get stiffed, remodeled, and/or degraded ending in maintaining tissue homeostasis. ECM could serve as the platform for cancer progression. The dysregulation of biochemical and biomechanical ECM features might take participate in some pathological conditions such as aging, tissue destruction, fibrosis, and particularly cancer. Tumors can reprogram how ECM remodels by producing factors able to induce protein synthesis, matrix proteinase expression, degradation of the basement membrane, growth signals and proliferation, angiogenesis, and metastasis. Therefore, targeting the ECM components, their secretion, and their interactions with other cells or tumors could be a promising strategy in cancer therapies. The present study initially introduces the physiological functions of ECM and then discusses how tumor‐dependent dysregulation of ECM could facilitate cancer progression and ends with reviewing the novel therapeutic strategies regarding ECM.
Effects of Rotation on Turbulence Production

Direct numerical simulations (DNS) of non-rotating and rotating turbulent channel flow were conducted. The data base obtained from these DNS simulations was used to investigate the prominent coherent structures involved in the turbulence generation cycle. Predictions from three theoretical models concerning the formation and evolution of sublayer streaks, three-dimensional hairpin vortices and propagating plane waves were validated using visualizations from the present DNS data. Quadrant analysis was used to determine a phase shift between the fluctuating streamwise and wall-normal velocities as a characteristic of turbulence production in the suction region at a low rotation number.
Impact of independent multidisciplinary work on the survival rate of stage 3 and 4 nasopharyngeal cancer in Indonesia: a retrospective cohort study

Background: The utilization of a multidisciplinary team (MDT) strategy is a beneficial approach in integrating the knowledge and proficiencies of various fields to produce thorough and advantageous cancer treatment plans for patients. Nevertheless, MDT has yet to be widely adopted in Indonesia. In this study, the authors examined an early form of MDT in Indonesia that does not involve dedicated meetings, referred as independent multidisciplinary work (IMW). The objective is to investigate the differences in survival rates of nasopharyngeal cancer (NPC) patients who underwent treatment with and without IMW. Materials and methods: This study has a retrospective cohort design. Data were collected from the medical records of newly diagnosed stage 3 and 4 NPC patients between 2016 and 2018. The subjects were divided into two groups: the IMW group and the non-IMW group. The primary end point of the study is overall survival rate between the two groups. Kaplan–Meier survival analysis, log-rank test, and cox proportional hazard analysis were used for statistical analysis. Results: This study included a total of 124 patients with NPC, 81 patients in the IMW group and 43 patients in the non-IMW group. At the end of the 36-month follow-up period, the median survival of the IMW group was not reached, while in the non-IMW, it was 12 months [95% confidence intervals (95% CI), 8.78–15.22], hazard ratio (HR): 0.47 (95% CI, 0.28–0.78; P<0.01). The 1-year survival rate was 66.7% in the IMW group versus 46.5% in the non-IMW group (HR=0.7, 95% CI 0.49–0.99; P=0.03); the 2-year survival rate was 40.7% in the IMW group versus 16.3% in the non-IMW group (HR=0.4, 95% CI 0.19–0.83; P<0.01). Daniel Rizky, Vina Yunarvika, and Yasjudan Rastrama Putra, these authors contributed equally to this work. In the multivariate analysis, the IMW approach, ECOG (The Eastern Cooperative Oncology Group) status, distant metastasis, and treatment approach were significantly associated with survival outcome. Conclusion: The use of IMW approach in the treatment of NPC was associated with a better survival outcome compared to non-IMW treatment.
Gross alpha, gross beta and Ra-226 activity concentrations in bottled mineral waters sold in Bangkok Metropolitan area and their committed effective dose

The activity concentrations of gross alpha, gross beta, and Ra-226 in some bottled mineral water samples sold in Bangkok Metropolitan area were measured from the view point of internal radiation for Thai consumers. Concentration ranges of gross alpha, gross beta, and Ra-226 in imported bottled mineral, domestic bottled mineral, and domestic bottled drinking water were as follows; for gross alpha, N.D. to 0.09, N.D. to 0.58, and N.D. to 0.17 Bq.l-1; for gross beta, N.D. to 0.44, N.D. to 0.45, and N.D. to 0.19 Bq.l-1; for Ra-226, 0.51 to 5.919, 0.422 to 9.915, and 0.096 to 0.398 mBq.l-1, respectively. On average, brands of domestic bottled mineral water contained the highest Ra-226 activity concentrations compared to those of imported bottled mineral and domestic bottled drinking water. However, all samples contained Ra-226 at concentration lower than the regulation limit for drinking water regulated by Thai Industrial Standard Institute (TISI), World Health Organization (WHO) and US. Environmental Protection Agency (US.EPA). The annual effective dose from intake of Ra-226 in waters to Thai different age groups (<1-17 y of age, and adults) was estimated. Group of infants and teens (10-17 y of age) child received the highest dose compared to all age group from the intake of Ra-226 in domestic bottled mineral water. These doses were approximately 25 times of that from intake of Ra-226 in domestic bottled drinking water and corresponded to 5.67-17% of the annual human exposure to radiation from water consumption (100 μSv) recommended by UNSCEAR (UNSCEAR 1993). Thus even though mineral waters contain useful elements needed especially during the period of maximum growth and development they can increase the risk of high exposures to human body due to ingest of Ra-226. Based on this study, while consumption is not a severe problem now, quality inspection of drinking water should be continued. Key words : Gross alpha, gross beta, Ra-226, bottled mineral waters, committed effective dose
Demographic, Medical, and Clinical Characteristics of a Population-Based Sample of Patients With Long-lasting Locked-In Syndrome

Background and Objectives Patients with classic locked-in syndrome (LIS), typically caused by ventral pontine stroke, present with quadriplegia, mutism, intact consciousness, and communication skills limited to vertical gazing and/or blinking. Clinical presentations and definitions of LIS differ, especially regarding incomplete LIS. In our study, we explored the functional diversity of LIS, its outcomes, and the complexity of its course along with variations in the location of lesions and their potential significance for outcomes. Methods A national cohort of patients with vascular LIS who remained in the LIS state for at least 6 weeks according to a functional definition of LIS was studied. Demographic, medical, and follow-up data, collected between 2012 and 2022, were obtained from the quality register of the Norwegian National Unit for Rehabilitation of Locked-In Syndrome. Outcomes in verbal communication, motor function, and dependency were evaluated according to criteria for being in or not in the LIS state. The modified Rankin scale and LIS motor recovery scale were applied. Descriptive analysis was performed. The relationship between lesion location and functional outcome was investigated. Results The sample included 51 patients (median age: 55.7 years, 36 male individuals), 43 of whom had follow-up data. Ischemic stroke was the most common etiology (n = 35). Twenty-three patients had emerged from the LIS state, mostly within 2 years after onset. All but 1 patient achieved some motor improvement, whereas only 3 achieved full motor recovery, and 88% had a persistently high level of dependence. The 3-year survival rate was 87%. Five patients had an isolated pontine lesion, whereas 80% showed various lesions outside the brain stem. Patients who emerged from the LIS state had a significantly lower prevalence of lesions outside the brain stem than patients who remained in the LIS state did. Discussion Investigating an unselected population-based sample of patients with vascular LIS offers important insights into the functional diversity of LIS. Although most patients remained severely disabled, even small improvements in function can substantially increase the potential for activity and participation. Additional lesions outside the brain stem seem to be common in long-lasting LIS and might be prognostic for remaining in the LIS state.
OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology

We present a simple low-cost system for comprehensive functional characterization of cardiac function under spontaneous and paced conditions, in standard 96 and 384-well plates. This full-plate actuator/imager, OptoDyCE-plate, uses optogenetic stimulation and optical readouts of voltage and calcium from all wells in parallel. The system is validated with syncytia of human induced pluripotent stem cell derived cardiomyocytes, iPSC-CMs, grown as monolayers, or in quasi-3D isotropic and anisotropic constructs using electrospun matrices, in 96 and 394-well format. Genetic modifications, e.g. interference CRISPR (CRISPRi), and nine compounds of acute and chronic action were tested, including five histone deacetylase inhibitors (HDACis). Their effects on voltage and calcium were compared across growth conditions and pacing rates. We also demonstrated deployment of optogenetic cell spheroids for point pacing to study conduction in 96-well format, and the use of temporal multiplexing to register voltage and calcium simultaneously on a single camera in this stand-alone platform. Opto-DyCE-plate showed excellent performance even in the small samples in 384-well plates, in the various configurations. Anisotropic structured constructs may provide some benefits in drug testing, although drug responses were consistent across tested configurations. Differential voltage vs. calcium responses were seen for some drugs, especially for non-traditional modulators of cardiac function, e.g. HDACi, and pacing rate was a powerful modulator of drug response, highlighting the need for comprehensive multiparametric assessment, as offered by OptoDyCE-plate. Increasing throughput and speed and reducing cost of screening can help stratify potential compounds early in the drug development process and accelerate the development of safer drugs.
E-cigarette vapour induces cellular senescence in primary lung fibroblasts and may contribute to lung pathology

Cellular senescence has been recognized to play a role in COPD pathophysiology. Non-aerosolized E-liquid treatment of a lung fibroblast foetal cell line resulted in cellular senescence induction, but this has not been assessed using E-vapour exposure and using primary lung fibroblasts. Therefore, we investigated whether E-vapour exposure induces cellular senescence in primary human lung fibroblasts and whether this affects their tissue repair function. Primary human lung fibroblasts were stimulated with E-cigarette vapour extracts, and cigarette smoke extract (CSE) and Paraquat (PQ) as positive controls. IL-8 secretion was measured to confirm a stimulatory response. Multiple senescence markers (p16, p21, SA-β-gal, and proliferation) were assessed and the tissue repair function was assessed using a scratch assay. Finally, we tried to validate our findings in an E-cigarette-exposed mouse model. Upon stimulation with CSE, PQ, and E-vapour extracts, cellular senescence was induced, which seemed dose-dependent and nicotine-independent. E-vapour-induced senescence resulted in an impaired tissue repair function. No significant difference was observed in the E-cigarette-exposed mouse model. In this study, we identify E-cigarette vapours’ potential to induce cellular senescence in primary human lung fibroblasts and that this affects their tissue repair function, which further adds to the identified risks of E-cigarette use.
Transcriptional Dysregulation and Impaired Neuronal Activity in FMR1 Knock-Out and Fragile X Patients’ iPSC-Derived Models

The lack of fragile X mental retardation protein (FMRP) protein, due to a repression of the FMR1 gene, causes Fragile X syndrome (FXS), one of the most prevalent forms of syndromic autisms. The FMR1 gene codes for an RNA binding protein involved in the regulation of gene expression through RNA processing, control of local translation, and protein-protein interactions; processes that are crucial for proper brain development. Taking advantage of induced pluripotent stem cells (iPSCs) and CRISPR-Cas9 genome editing technologies, we generated iPSC-derived cortical neural progenitors and cortical neurons from an FMR1 knock-out and patient cell line with the aim of identifying common phenotypes between the two cellular models. Using RNA sequencing, quantitative PCR and multielectrode array approaches, we assessed how the absence of the functional FMR1 gene affects the transcriptional profiles and the activities of iPSC-derived cortical neuronal progenitor cells (NPCs) and neurons with both models. We observed that FMR1 KO and FXS patient cells have a decrease in their mean firing rate; a cellular activity that can also be blocked by tetrodotoxin (TTX) application in wild-type active neurons. Relative to wild-type neurons, in FMR1 KO neurons, increased expression of presynaptic mRNA and transcription factors involved in the forebrain specification and decreased levels of mRNA coding AMPA and NMDA subunits were observed. Intriguingly, 40% of the differentially expressed genes were commonly deregulated between NPCs and differentiating neurons with significant enrichments in FMRP targets and Autism Related Genes found amongst downregulated genes. This implies that an absence of functional FMRP affects transcriptional profiles at the NPC stage, resulting in impaired activity and differentiation of the progenitors into mature neurons over time. These findings from the FMR1 KO lines were also shared with FXS patients’ iPSC-derived cells that also present with an impairment in activity and neuronal differentiation, illustrating the critical role of FMRP protein in neuronal development.
A microfluidic model of colonocyte-microbiota interaction mimicking the colorectal cancer microenvironment

Changes in the abundance of certain bacterial species within the colorectal microbiota correlate with colorectal cancer development. While carcinogenic mechanisms of single pathogenic bacteria have been characterized in vitro, limited tools are available to investigate interactions between pathogenic bacteria and both commensal microbiota and colonocytes in a physiologically relevant tumor microenvironment. To address this, we developed a microfluidic device that can be used to co-culture colonocytes and colorectal microbiota. The device was used to explore the effect of Fusobacterium nucleatum, an opportunistic pathogen associated with colorectal cancer development in humans, on colonocyte gene expression and microbiota composition. F. nucleatum altered the transcription of genes involved in cytokine production, epithelial-to-mesenchymal transition, and proliferation in colonocytes in a contact-independent manner; however, most of these effects were diminished by the presence of fecal microbiota. Interestingly, F. nucleatum significantly altered the abundance of multiple bacterial clades associated with mucosal immune responses and cancer development in the colon. Our results highlight the importance of evaluating the potential carcinogenic activity of pathogens in the context of a commensal microbiota, and the potential to discover novel inter-species microbial interactions in the colorectal cancer microenvironment.
Individual differences in wellbeing are supported by separable sets of co-active self- and visual-attention-related brain networks

How does the brain support ‘wellbeing’? Because it is a multidimensional construct, it is likely the product of multiple co-active brain networks that vary across individuals. This is perhaps why prior neuroimaging studies have found inconsistent anatomical associations with wellbeing. Furthermore, these used ‘laboratory-style’ or ‘resting-state’ methods not amenable to finding manifold networks. To address these issues, we had participants watch a full-length romantic comedy-drama film during functional magnetic resonance imaging. We hypothesised that individual differences in wellbeing measured before scanning would be correlated with individual differences in brain networks associated with ‘embodied’ and ‘narrative’ self-related processing. Indeed, searchlight spatial inter-participant representational similarity and subsequent analyses revealed seven sets of co-activated networks associated with individual differences in wellbeing. Two were ‘embodied self’ related, including brain regions associated with autonomic and affective processing. Three sets were ‘narrative self’ related, involving speech, language, and autobiographical memory related regions. Finally, two sets of visual-attention related networks emerged. These results suggest that the neurobiology of wellbeing in the real-world is supported by diverse but functionally definable and separable sets of networks. This has implications for psychotherapy where individualised interventions might target, e.g., neuroplasticity in language-related narrative over embodied self or visual-attentional related processes. Significance Statement Humans deeply care about their own and others wellbeing. Yet, the role the brain plays in generating it remains unestablished, perhaps because wellbeing means different things to different people. Here, we asked whether individual differences in wellbeing are associated with differences in brain networks supporting ‘embodied’ and ‘narrative’ selves. That is, how people feel in their bodies and their emotions might contribute more to wellbeing in some individuals. In others, the stories people tell about themselves might be a more important determinant. Supporting this, we found that different self-ratings of wellbeing are associated with different embodied and narrative self-related networks. We also found differences in networks associated with visual-attention, suggesting that what people attend to in the visual world also contributes to wellbeing. These results have implications for how mental health professionals and other individuals might intervene to improve wellbeing by differentially focusing on embodied-self, narrative-self, or visual-attentional related processes.
Metabolites with benzene ring from sugarcane leaf play important role in plant-Spodoptera frugiperda interaction

Secondary metabolism plays important role in plant growth and development, however, the relationship between secondary metabolism and adaptive plant-insect communication is largely unknown. The present study used sugarcane line highly susceptible to Spodoptera frugiperda and sister line with medium resistance to analyze the role of plant non-volatile organic compounds (NOCs) and volatile organic compounds (VOCs) in sugarcane-S. frugiperda interaction. A total of 46 plant NOCs and 15 plant VOCs significantly different between resistant and susceptible lines and were continuously up-regulated and down-regulated at different time points before/after S. frugiperda treatment were screened. Phenolic acids containing benzene ring accounted for the largest proportion of differential NOCs. Levels of 66.7% of these phenolic acids were higher in susceptible line. Feeding supplemented with NOCs showed that phenoxyacetic acid (phenolic acid) and 4-methoxybenzaldehyde (aromatic phenolic acid) both increased the male-to-female ratio of S. frugiperda. Aromatics containing benzene ring, accounted for the largest of differential VOCs in susceptible line. Two aromatics, p-cymene and benzene and 1-ethenyl-4-methoxy-, with higher level in susceptible line, were attractive to S. frugiperda. Terpenoids, aldehyde, and esters accounted for most of higher-in-resistant VOCs, with most tested to be repellent to S. frugiperda. Furthermore, transcriptome analysis of S. frugiperda feeding on susceptible and resistant lines combined with feeding assays revealed that tryptophan, as a precursor of aromatic compounds that also contains benzene ring, could promote the growth and development of S. frugiperda in nutritional deficiency condition. These findings together suggested that benzene-ring containing compounds play a critical role in plant-Spodoptera frugiperda interaction.
Modelling the water quality in dams within the Umgeni Water operational area with emphasis on algal relations

Based on many years of water quality (including algal) and water treatment cost data, available at Umgeni Water, a study was undertaken to better understand the water quality relationships in man made lakes within the company's operational area, and to investigate how water quality affected the cost of treating water from these lakes. The broad aims to the study were to: identify the key environmental variables that were affecting algal populations in lakes; and if these were significant to establish predictive models relating algae to the water quality; and to develop models relating the water quality in lakes to the cost of treating water