Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic and Biochemical Characterization of the First Extended-Spectrum CARB-Type ß-Lactamase, RTG-4, from Acinetobacter baumannii (cid:1)

Acinetobacter baumannii isolate KAR was uncommonly more resistant to cefepime and cefpirome than to ceftazidime and cefotaxime. Cloning and expression of the (cid:1) -lactamase gene content of this isolate into Escherichia coli TOP10 identiﬁed ß-lactamase RTG-4 (or CARB-10), which corresponds to the ﬁrst reported extended-spectrum CARB-type enzyme. RTG-4 is a plasmid-encoded Ambler class A (cid:1) -lactamase whose sequence differs by 4 amino acid substitutions from the narrow-spectrum (cid:1) -lactamase RTG-3. RTG-4 hydrolyzes cefepime and cefpirome and weakly hydrolyzes ceftazidime due to the single Ser-to-Thr substitution at Ambler position 69. RTG-4 is less susceptible to inhibition by tazobactam and sulbactam than RTG-3. Expression of (cid:1) -lactamase RTG-4 in a wild-type A. baumannii reference strain showed that it conferred resistance to cefepime and cefpirome. The genetic environment of the bla RTG-4 gene was made of a peculiar transposon located on a ca. 50-kb plasmid. IS Aba9 , located upstream of bla RTG-4 , may be responsible for its acquisition by recognizing a secondary right inverted repeat sequence, thus acting by a one-ended transposition process.
Talent Identification in Professional Soccer Players According to Their Birth Date

: Some studies showed that most professional soccer players were born on the same date. The aim of this study was to investigate talent identification among players of the 2006 FIFA World Cup according to the distribution of their birth date in different playing positions in five continents of the world. Information about birth dates of 736 players was collected from FIFA website. Chi Squire test and ANOVA were used to assess the differences among the players' birth dates. The results showed no significant effect in different positions between players born in the first and second halves of month and between players born in different months (P>0.05). Although the births between August and October were more than other seasons, the difference was not significant (P>0.05). Also, no significant effect was found between different positions and the players' birth season (P>0.05). No significant effect was found between month and season of births in five continents (P>0.05). Although the mean of height and the mean of weight of the players born between August and October were more than other seasons, this difference was not significant (P>0.05). It can be concluded that birth date of players of the 2006 FIFA World Cup was similar to the distribution of playing positions and continents. Talent identification according to the birth date is not reliable.
OTT_A_323898 4853..4858

1Department of Urology, International University of Health and Welfare Narita Hospital, Narita, Japan; 2Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan; 3Department of Pathology, International University of Health and Welfare Narita Hospital, Narita, Japan Abstract: Testicular germ cell tumor (TGCT) is highly chemo-sensitive cancer; however, there is no established treatment for TGCT relapsed after multiple chemotherapy. Although pembrolizumab showed durable stable disease in some patients, no reliable biomarker for predicting response is available. High microsatellite instability (MSI) is rare in chemo-naïve TGCT. We report a TGCT patient with a rapid response to pembrolizumab. A 34-year-old Japanese male diagnosed with advanced TGCT underwent PCR-based testing of the primary site; it did not reveal MSI. He relapsed after four chemotherapy regimens: bleomycin, etoposide and cisplatin; paclitaxel, ifosfamide and cisplatin; vinblastine, ifosfamide and cisplatin; and irinotecan+nedaplatin with a total of 20 treatment cycles. Chemotherapy was thus discontinued. Re-examination by a CT-guided needle biopsy for progressing retroperitoneal lymph node (RPLN) metastases showed MSI-high; pembrolizumab was initiated. After only two doses, the human chorionic gonadotropin level decreased from 6500 to <1.0 IU/L. PET-CT showed shrinkage of the RPLN metastases with diminished metabolism. The patient is currently free from disease progression for 6 months from the start of pembrolizumab. This is the first report of refractory TGCT with MSI-high responding to pembrolizumab. We emphasize the utility of a metastatic-site biopsy to check the MSI status for refractory TGCT even when primary site is MSI-negative.
INTRA-INDUSTRY TRADE IN THE VISEGRAD COUNTRIES: DOES THE LINDER HYPOTHESIS HOLD?* countries and the Linder hypothesis

Visegrad In 1961, Staffan Linder attacked mainstream trade economics by diverging from the generally ac-cepted factor endowments theory and focusing on alternative explanations of why countries trade with each other. He was among the ﬁ rst economists to recognise the growing importance of intra-industry trade and presented his hypothesis that the more similar the per capita income levels of countries, the more they tend to trade with each other. This observation has since become one of the main pillars of modern trade theory. The present paper assesses the empirical validity of the Linder hypothesis in the Visegrad countries. Using a variant of the gravity model, it ﬁ nds that when controlling for other factors, the Visegrad countries tend to trade more with countries with similar per capita income levels than with signi ﬁ cantly richer or poorer countries. This observation is consistent with the Linder hypothesis. OLS regressions, Tobit regressions, and robustness checks all support the hypothesis.
All Three Variable Regions of the TRIM5 (cid:2) B30.2 Domain Can Contribute to the Speciﬁcity of Retrovirus Restriction

Recent studies have revealed the contribution of TRIM5 (cid:1) to retrovirus restriction in cells from a variety of primate species. TRIM5 (cid:1) consists of a tripartite motif (the RBCC domain) followed by a B30.2 domain. The B30.2 domain is thought to be involved in determination of restriction speciﬁcity and contains three variable regions. To investigate the relationship between the phylogeny of primate TRIM5 (cid:1) and retrovirus restriction speciﬁcity, a series of chimeric TRIM5 (cid:1) consisting of the human RBCC domain followed by the B30.2 domain from various primates was constructed. These constructs showed restriction proﬁles largely consistent with the origin of the B30.2 domain. Restriction speciﬁcity was further investigated with a variety of TRIM5 (cid:1) s containing mixed or mutated B30.2 domains. This study revealed the importance of all three variable regions for determining restriction speciﬁcity. Based on the molecular structures of other PRYSPRY domains solved recently, a model for the molecular structure of the B30.2 domain of TRIM5 (cid:1) was developed. The model revealed that the variable regions of the B30.2 domain are present as loops located on one side of the B30.2 core structure. It is hypothesized that these three loops form a binding surface for virus and that evolutionary changes in any one of the loops can alter restriction speciﬁcity.
Ecosystem Services Provided by Cover Crops and Biofumigation in Sunﬂower Cultivation

: In south-western France, sunﬂowers are usually grown in short rotations and after a long fallow period during which soils are left bare. This practice can favour diseases, caused by soilborne fungi, such as sunﬂower verticillium wilt (SVW), as well as nitrate leaching, both of which can decrease yields. Growing cover crops during a fallow period is an agroecological practice that could provide ecosystem services and mitigate SVW. A Brassicaceae cover crop that causes biofumigation produces glucosinolates and liberate biocidal compounds that might regulate SVW biologically. Moreover, the green manure effect of the Fabaceae might increase soil mineral nitrogen (SMN). To go further, mixtures of Brassicaceae and Fabaceae might mutualise the beneﬁts that each cover crop has in sole crops. A four-year ﬁeld study in south-western France tested Brassicaceae (brown mustard, turnip rape and fodder radish) and Fabaceae (purple vetch) in sole crops or a mixture with two or three species during the fallow period, followed by biofumigation, and sunﬂower crop. The cover crops were characterised, SMN was measured, the SVW and yield were assessed and compared to those of the crop grown on soils left bare during the fallow period. Purple vetch as a sole crop cover crop signiﬁcantly increased SMN before sunﬂower sowing but only in 2019. Fodder radish as a sole crop reduced SVW severity signiﬁcantly, overall, 80 days after sowing, except in 2019, when weather conditions were unfavourable to biofumigation. Purple vetch as a sole crop also reduced signiﬁcantly SVW severity in 2017 and 2020. Finally, sunﬂower yields after cover crops were higher than those after bare soils, only after purple vetch as a sole crop in 2020, with a mean increase of 0.77 t ha − 1 . Mixtures of Brassicaceae and Fabaceae sowed at these densities resulted in an intermediate SMN level between those in sole crops and the bare soil and they did not signiﬁcantly decrease SVW or increase yields. Our results suggest that cover crops, especially fodder radish or purple vetch, and biofumigation can provide ecosystem services for sunﬂower, instead of leaving soils bare during the fallow period. However, the extent of ecosystem services is markedly affected by soil and climatic conditions as well as other management practices.
Research Online Research Online Loosely-coupled consistency between agent-oriented conceptual models Loosely-coupled consistency between agent-oriented conceptual models and Z specifications and Z specifications

Abstract Agent-oriented conceptual modelling (AOCM) is a relatively new technique that offers significant benefits in the modelling and development of complex computer systems. It is highly effective in answering questions such that what are the main goals of the system, how key actors depend on each other, and what alternatives exist. A formal method can benefit any stage of the software development lifecycle and improves the quality of the computer systems. The paper defines an approach that allows to complement requirements modelling notations with formal specifications, while preserving the consistency between them. Abstract Agent-oriented conceptual modelling (AOCM) is a relatively new technique that offers signiﬁcant beneﬁts in the modelling and development of complex computer systems. It is highly effective in answering questions such that what are the main goals of the system, how key actors depend on each other, and what alternatives exist. A formal method can beneﬁt any stage of the software development lifecycle and improves the quality of the computer systems. The paper deﬁnes an approach that allows to complement requirements modelling notations with formal speciﬁcations, while preserving the consistency between them.
Edinburgh Explorer The impact of culture on mindreading

The role of culture in shaping folk psychology and mindreading has been neglected in the philosophical literature. This paper shows that there are signiﬁcant cultural differences in how psychological states are understood and used by (1) drawing on Spaulding’s recent distinction between the ‘goals’ and ‘methods’ of mindreading (2018) to argue that the relations between these methods vary across cultures; and (2) arguing that differences in folk psychology cannot be dismissed as irrelevant to the cognitive architecture that facilitates our understanding of psychological states. The paper concludes that any good account of social cognition must have the conceptual resources to explain how culture affects our understanding of psychological states, and that this explanandum should not be an after-thought but instead a guiding feature for those accounts.
Non-Essential Cluster Values and Normal Functions

We consider continuous functions f which map the open unit disk D into the Riemann sphere W. For a point ζ on the unit circle C, we say that χ is a chord at ζ if χ is a chord of C having one endpoint at ζ and that Δ is a Stolz angle at ζ if Δ is a Stolz angle with vertex ζ. Suppose S denotes either a chord at ζ, a Stolz angle at ζ, or D.
Investigating Achievement Goals for Egyptian Nursing Categories

This research focuses on Egyptian nursing students' achievement goals toward their studies during their academic studies. It aimed to investigate the achievement goals of nursing students. Nursing students ‘ adoption levels of achievement goals are described. Also, put forth the achievement goal orientations of nursing categories (secondary students, faculty students). Achievement Goals Orientations Scale was distributed to 175nursing students (94 faculty, 46 males and 48 females- 81 secondary, females only). The findings showed that firstly, students generally adopted all achievement goal orientations at a medium level. And order of them for nursing students were performance-avoidance, performance-approach, mastery –avoidance then mastery-approach orientation. Secondly, faculty students have higher mastery-approach goal and lower performance-avoidance goal than secondary students. Also, Females in faculty have higher mastery-approach goal, performance-approach goal and lower performance-avoidance than females in secondary school. Finally, males have higher mastery-approach goal and lower performance-approach goal than females.
High-Temperature Structural and Electrical Properties of Ba Ln Co 2 O 6 Positrodes

: The application of double perovskite cobaltites Ba Ln Co 2 O 6 − δ ( Ln = lanthanide element) in electrochemical devices for energy conversion requires control of their properties at operating conditions. This work presents a study of a series of Ba Ln Co 2 O 6 − δ ( Ln = La, Pr, Nd) with a focus on the evolution of structural and electrical properties with temperature. Symmetry, oxygen non-stoichiometry, and cobalt valence state have been examined by means of Synchrotron Radiation Powder X-ray Di ﬀ raction (SR-PXD), thermogravimetry (TG), and X-ray Absorption Spectroscopy (XAS). The results indicate that all three compositions maintain mainly orthorhombic structure from RT to 1000 ◦ C. Chemical expansion from Co reduction and formation of oxygen vacancies is observed and characterized above 350 ◦ C. Following XAS experiments, the high spin of Co was ascertained in the whole range of temperatures for BLC, BPC, and BNC.
at UWindsor Psychological Help-Seeking among Latin American Immigrants in Canada: Testing a Culturally-Expanded Model of the Theory of Reasoned Action Using Path Analysis

The current study investigated the psychosocial and cultural predictors of psychological help-seeking based on the Theory of Reasoned Action (TRA: Ajzen and Fishbein 1980) in a sample of 223 adult Latin American immigrants living in Canada. Using path analysis, the results provided empirical support for the TRA, as both help-seeking attitudes and subjective norms were found to influence participants ’ help-seeking intentions. Moreover, the re-specified culturally-expanded model showed a good fit to the data and revealed the direct and indirect effects that bi-directional acculturation (Latino and Canadian Cultural orienta-tions), familism, and collective coping had on help-seeking intentions. The results point to the integral roles familism and subjective norms of social referents (e.g., parents, siblings) play in the help-seeking decision process among Latin Americans. Limitations, future research direc-tions and clinical implications are considered and discussed.
Inﬂuence of Acidiﬁed Biochar on CO 2 –C Efﬂux and Micronutrient Availability in an Alkaline Sandy Soil

: Biochar, an alkaline carbonaceous substance resulting from the thermal pyrolysis of biomass, reportedly enhances the micronutrient availability in acidic soils with little or no effect on alkaline soils. In this study, biochars were produced from poultry manure (PM) at 350 ◦ C and 550 ◦ C (BC350 and BC550 respectively). The acidiﬁed biochars (ABC350 and ABC550, respectively) were incorporated into an alkaline sandy soil, and their effects on the soil micronutrients (Cu, Fe, Mn and Zn) availability, and CO 2 –C efﬂux were investigated in a 30-day incubation study. The treatments (PM, BC350, BC550, ABC350, and ABC550) were administered in triplicate to 100 g soil at 0%, 1%, and 3% ( w / w ). Relative to the poultry manure treatment, acidiﬁcation drastically reduced the pH of BC350 and BC550 by 3.13 and 4.28 units, respectively, and increased the micronutrient availability of the studied soil. Furthermore, the biochars (both non-acidiﬁed and acidiﬁed) reduced the CO 2 emission compared to that of the poultry manure treatment. After 1% treatment with BC550 and ABC550, the CO 2 emissions from the soil were 89.6% and 91.4% lower, respectively, than in the 1% poultry manure treatment. In summary, acidiﬁed biochar improved the micronutrient availability in alkaline soil, and when produced at higher temperature, can mitigate the CO 2 emissions of soil carbon sequestration.
Selective effect of MNK on mRNA translation

The mitogen-activated protein kinaseinteracting kinases 1 and 2 (MNK1 and MNK2) are activated by extracellular-signal-regulated kinases 1 and 2 (ERK1/2) or p38 in response to cellular stress and extracellular stimuli that include growth factors, cytokines, and hormones. Modulation of MNK activity affects translation of mRNAs involved in the cell cycle, cancer progression, and cell survival. However, the mechanism by which MNK selectively affects translation of these mRNAs is not understood. MNK binds eIF4G and phosphorylates the cap-binding protein eIF4E. Using a cell-free translation system from rabbit reticulocytes programmed with mRNAs containing different 5’-ends, we show that a MNK inhibitor, CGP57380, affects translation of only those mRNAs that contain both a cap and a hairpin in the 5’-untranslated region (UTR). Similarly, a C-terminal fragment of human eIF4G-1, eIF4G(1357-1600), which prevents binding of MNK to intact eIF4G, reduces eIF4E phosphorylation and inhibits translation of only capped and hairpincontaining mRNAs. Analysis of proteins bound to m 7 GTP-Sepharose reveals that both CGP and eIF4G(1357-1600) decrease binding of eIF4E to eIF4G. These data suggest that MNK stimulates translation only of mRNAs containing both a cap and 5’-terminal RNA duplex via eIF4E phosphorylation, thereby enhancing the coupled cap-binding and RNA-unwinding activities of
Na and Na /NH exchange activities of zebraﬁsh NHE3b expressed in Xenopus oocytes

Na /H Na NH 4 (cid:2) exchange activities of zebraﬁsh NHE3b expressed in Xenopus oocytes. Am Physiol 2014. Na (cid:2) /H (cid:2) exchanger 3b (zNHE3b) is highly expressed in the apical membrane of ionocytes where Na (cid:2) is absorbed from ion-poor fresh water against a concentration gradient. Much in vivo data indicated that zNHE3b is involved in Na (cid:2) absorption but not leakage. However, zNHE3b-mediated Na (cid:2) absorption has not been thermodynamically explained, and zNHE3b activity has not been measured. To address this issue, we overexpressed zNHE3b in Xenopus oocytes and characterized its activity by electrophysiology. Exposure of zNHE3b oocytes to Na (cid:2) -free media resulted in signiﬁcant decrease in intracellular pH (pH i ) and intracellular Na (cid:2) activity ( a Na i ). a Na i increased signiﬁcantly when the cytoplasm was acidiﬁed by media containing CO 2 -HCO 3 (cid:3) or butyrate. Activity of zNHE3b was inhibited by amiloride or 5-ethylisopropyl amiloride (EIPA). Although the activity was accompanied by a large hyperpolarization of (cid:4) 50 mV, voltage-clamp experiments showed that Na (cid:2) /H (cid:2) exchange activity of zNHE3b is electroneutral. Exposure of zNHE3b oocytes to medium containing NH 3 /NH 4 (cid:2) resulted in signiﬁcant decreases in pH i and a Na i and signiﬁcant increase in intracellular NH 4 (cid:2) activity, indicating that zNHE3b mediates the Na (cid:2) /NH 4 (cid:2) exchange. In low-Na (cid:2) (0.5 mM) media, zNHE3b oocytes maintained a Na i of 1.3 mM, and Na (cid:2) -inﬂux was observed when pH i was decreased by media containing CO 2 - HCO 3 (cid:3) or butyrate. These results provide thermodynamic evidence that zNHE3b mediates Na (cid:2) absorption from ion-poor fresh water by its Na (cid:2) /H (cid:2) and Na (cid:2) /NH 4 (cid:2) exchange activities. Quantitative compared between and control and the statistical signiﬁcances P were calculated by unpaired two-sided Student’s t -test Prism
Brief Report Investigating the infant gut microbiota in developing countries: worldwide metagenomic meta-analysis involving infants living in sub-urban areas of Côte d ’ Ivoire

In recent decades, infants ’ gut microbiota has aroused constant scienti ﬁ c interest, primarily due to early- and long-term repercussions on the host health. In this context, nutritional challenges such as those found in less developed countries can in ﬂ uence infants ’ gut microbiota development, thus gen-erating potentially critical health outcomes. However, comprehensive investigations regarding species-level differences in the infant gut microbiota ’ s composition between urbanized and rural countries are still missing. In this study, 16S rRNA and Shallow Shotgun metagenomics sequencing were exploited to dissect the microbial community ’ s species-level composition of 11 faecal samples collected from infants living in a semi-urban area of Sub-Saharan Africa, i.e. Côte d ’ Ivoire. Moreover, the generated data were coupled with those retrieved from public available metagenomic repositories, including two rural communities and 13 urban communities of industrialized countries. The meta-analysis led to the identi ﬁ cation of Infant Species Community States Type (ISCSTs) and microbial species covariances, which were exploited to reveal key signatures of infants living in rural and semi-urban societies. Remarkably, analysis of rural and semi-urban datasets revealed shifts from ISCSTs prevalent in urbanized populations with putative health implications. Thus, indicating the need for population-wide investigations aimed to de ﬁ ne the factors determin-ing such potentially harmful gut microbial communities ’ signatures.
EFFECTS OF OAK POWDERY MILDEW (Erysiphe alphitoides [GRIFFON AND MAUBL.] U. BRAUN AND S. TAKAM.) ON PHOTOSYNTHESIS OF PEDUNCULATE OAK (Quercus robur L.)

The aim of the present study was to evaluate the effect of one of the most important foliar diseases, powdery mildew, on the leaf physiological traits of Quercus robur L. using chlorophyll a fluorescence parameters in combination with parameters of leaf gas exchange. For this purpose, greenhouse semi-controlled experiment was conducted with 25 one-year-old seedlings kept in optimal conditions, and the same number of seedlings infected with the mentioned pathogen. Measurements were carried out when the coverage of epiphytic micelia visually reached more than 75% of the surface of leaves in the infected seedlings. The results of gas exchange measurement showed that Erysiphe alphitoides (Griffon and Maubl.) U. Braun and S. Takam caused a significant reduction of net photosynthesis (A) and a significant increase in substomatal CO2 concentration (Ci). Furthermore, considering the fast kinetics of chlorophyll a fluorescence, all of the observes parameters were significantly affected by oak powdery mildew. On the other hand, pulse amplitude modulated fluorescence parameters were mildly affected, with only minimal (Fo’) and maximal (Fm’) fluorescence of dark adopted leaves showing significant difference. This study presented the possibility of usage and the effectiveness of chlorophyll a fluorescence parameters in detection the severe stress conditions, on the example of leaves infected with oak powdery mildew over 75%. Some additional studies should be conducted in the future to determine the possibility of usage and the effectiveness of the observed fluorescence parameters of fast kinetics in detection of mild and early stress.