Miracle-dz/UniFAR-FTUs huggingface dataset:
Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

Altered signaling between gut bacteria and their host has recently been implicated in the pathophysiology of eating disorders, whereas the enterobacterial caseinolytic protease B (ClpB) may play a key role as an antigen mimetic of α-melanocyte-stimulating hormone, an anorexigenic neuropeptide. Here, we studied whether ClpB production by gut bacteria can be modified by chronic food restriction and female sex, two major risk factors for the development of eating disorders. We found that food restriction increased ClpB DNA in feces and ClpB protein in plasma in both male and female rats, whereas females displayed elevated basal ClpB protein levels in the lower gut and plasma as well as increased ClpB-reactive immunoglobulins (Ig)M and IgG. In contrast, direct application of estradiol in E. coli cultures decreased ClpB concentrations in bacteria, while testosterone had no effect. Thus, these data support a mechanistic link between host-dependent risk factors of eating disorders and the enterobacterial ClpB protein production.
Pain Relief Following Sternotomy in Conventional Cardiac Surgery: A Review of Non Neuraxial Regional Nerve Blocks

Acute post-operative pain following sternotomy in cardiac surgery should be adequately managed so as to avoid adverse hemodynamic consequences and pulmonary complications. In the era of fast tracking, adequate and efficient technique of post-operative analgesia enables early extubation, mobilization and discharge from intensive care unit. Due to increasing expertise in ultrasound guided blocks there is a recent surge in trial of bilateral nerve blocks for pain relief following sternotomy. The aim of this article was to review non-neuraxial regional blocks for analgesia following sternotomy in cardiac surgery. Due to the paucity of similar studies and heterogeneity, the assessment of bias, systematic review or pooled analysis/meta-analysis was not feasible. A total of 17 articles were found to be directly related to the performance of non-neuraxial regional nerve blocks across all study designs. Due to scarcity of literature, comments cannot be made on the superiority of these blocks over each other. However, most of the reviewed techniques were found to be equally efficacious or better than conventional and established techniques.
Transplantation of adipose tissue lacking PAI-1 improves glucose tolerance and attenuates cardiac metabolic abnormalities in high-fat diet-induced obesity

ABSTRACT Adipose tissue is an important metabolic organ, and transplantation of white adipose tissue plays crucial roles in glucose homoeostasis and energy metabolism. However, how adipose tissue affects glucose utilization is poorly understood. PAI-1-knockout (PAI-1KO) mice were previously shown to be resistant to a high-fat diet and obesity. We used microPET/CT (positron emission tomography/computed tomography), gene microarray, and biochemical assays to measure changes in systemic and myocardial glucose metabolism in mice subjected to transplantation of adipose tissue from PAI-1KO and wild-type mice. Here, we show that transplanting subcutaneous white adipose tissue (scWAT) from PAI-1KO mice into high-fat diet (HFD)-fed mice reduced levels of serum total cholesterol and triglycerides, and improved glucose tolerance in the HFD-fed mice. microPET/CT imaging revealed that cardiac glucose uptake was increased in the heart but not in the liver, hindlimb muscles, or abdominal subcutaneous white adipose tissue in HFD-fed mice transplanted with PAI-1KO scWAT, suggesting that the transplanted PAI-1KO scWAT exerted endocrine effects in the heart. In addition, transplantation of scWAT from PAI-1KO mice upregulated mitochondrial gene expression in cardiac muscle, increased the expression of glucose transporters 1 and 4 in cardiac tissues and was associated with an increased NAD+/NADH ratio. Together, these findings suggest that modulating PAI-1 in scWAT may provide a promising approach for intervening in glucose metabolism.
Importance and Diagnosis of Flexibility Preparation of Male Sport Climbers

The objective of the study was to verify the relationships between sport skill levels and to identify the tests that accurately diagnose flexibility of sport climbers. This study examined 60 competitive advanced–higher elite male 7b–9a redpoint (RP) climbers. The athletes performed commonly used flexibility tests (stand-and-reach, straddle sit, straddle stand) and climbing-specific flexibility tests. Significant correlations were found between sport skill levels for the straddle stand test (r = −0.48) and the straddle sit test (r = −0.41). No significant correlations were observed between climbing-specific flexibility tests and sports skill level of climbers. Hip abduction evaluated using the straddle sit and straddle stand tests were significantly correlated with sports skill level and thus can be approached as a tool to diagnose flexibility of climbers. Flexibility is very specific and difficult to diagnose in climbing, but it should be developed.
The Effect of Solution-Focused Group Counseling Intervention on College Students’ Internet Addiction: A Pilot Study

This pilot study aimed to explore the effect of solution-focused group counseling intervention on Internet addiction among college students. Eighteen college students participated in this study, out of which nine subjects were assigned into the experimental group and the rest (n = 9) to a control group. The experimental group received group counseling for five weeks, while the control group did not receive any intervention. The revised version of the Chinese Internet Addiction Scale (CIAS-R) was used to capture pre-test and post-test excessive use in the two groups. The experimental group was also subjected to a follow-up test and self-reported Internet addiction scores six months after the end of group counseling. Results showed that after the five-week solution-focused group counseling, the scores of four dimensions of the CIAS-R in the experimental group had CIAS-R decreased, and the reduction trend of the total score of CIAS-R was similar across all subjects in this group. The treatment effect was larger than the placebo reduction in the control group in two dimensions: compulsive and withdrawal (Sym-C & Sym-W) and tolerance (Sym-T) symptoms. Qualitative research confirmed the conclusions from the quantitative data, showing that the experimental group reduced its Internet addiction symptoms. Overall, the findings suggested that solution-focused group counseling had positive intervention effects on Internet addiction.
PHIP drives glioblastoma motility and invasion by regulating the focal adhesion complex

Significance Glioblastoma has an extremely poor prognosis, driven by its invasive and angiogenic potential. The development of effective therapies for glioblastoma will require the identification of molecular factors that promote these hallmarks. Our results show that PHIP drives glioblastoma motility, invasion, and angiogenesis. These functions are enabled by localization of PHIP to focal adhesions. PHIP regulates expression of focal adhesion proteins and physically interacts with vinculin, mechanisms by which it promotes tumor cell motility and invasion. The presence of elevated PHIP copy number in distinct molecular glioblastoma subtypes provides additional support for its importance to glioblastoma biology. These studies also identify PHIP as a compelling target for glioblastoma therapy. The invasive behavior of glioblastoma is essential to its aggressive potential. Here, we show that pleckstrin homology domain interacting protein (PHIP), acting through effects on the force transduction layer of the focal adhesion complex, drives glioblastoma motility and invasion. Immunofluorescence analysis localized PHIP to the leading edge of glioblastoma cells, together with several focal adhesion proteins: vinculin (VCL), talin 1 (TLN1), integrin beta 1 (ITGB1), as well as phosphorylated forms of paxillin (pPXN) and focal adhesion kinase (pFAK). Confocal microscopy specifically localized PHIP to the force transduction layer, together with TLN1 and VCL. Immunoprecipitation revealed a physical interaction between PHIP and VCL. Targeted suppression of PHIP resulted in significant down-regulation of these focal adhesion proteins, along with zyxin (ZYX), and produced profoundly disorganized stress fibers. Live-cell imaging of glioblastoma cells overexpressing a ZYX-GFP construct demonstrated a role for PHIP in regulating focal adhesion dynamics. PHIP silencing significantly suppressed the migratory and invasive capacity of glioblastoma cells, partially restored following TLN1 or ZYX cDNA overexpression. PHIP knockdown produced substantial suppression of tumor growth upon intracranial implantation, as well as significantly reduced microvessel density and secreted VEGF levels. PHIP copy number was elevated in the classical glioblastoma subtype and correlated with elevated EGFR levels. These results demonstrate PHIP’s role in regulating the actin cytoskeleton, focal adhesion dynamics, and tumor cell motility, and identify PHIP as a key driver of glioblastoma migration and invasion.
Prevalence of asymptomatic malaria in HIV-infected subjects on cotrimoxazole antimalarial prophylaxis attending a tertiary health care center in southern Nigeria: a cross-sectional study.

Introduction Co-infection has become a major contributor to increased burden of morbidity and mortality in HIV infection. The aim of this study was to assess the prevalence of asymptomatic malaria in the HIV-infected subjects on antimalarial prophylaxis and provide information to improve management of HIV subjects. Methods This was a cross-sectional study with a purposive sampling. Microscopy method was used for the confirmation of malaria parasitemia status. The study was performed in University of Calabar Teaching Hospital, Calabar, Cross River State, Nigeria, a major tertiary health institution within the period of January to June 2016 involving 100 participants. Results The majority (65%) of the study participants were females. The majority of the studied population belonged to the age range 33-38 years old. Most (45%) of the patients had CD4 count ≥500 cells/µL. The prevalence of asymptomatic malaria was found to be 13% (13/100). The distribution of asymptomatic malaria based on gender and age were found not to be statistically significant (P>0.05). Subjects with CD4 count in the range of 200-499 cells/µL had the highest prevalence (24.39%) of asymptomatic malaria. Conclusions Considering that all the studied participants were on antimalarial prophylaxis, it signals a public health concern to employ more intensive preventive methods in addition to antimalaria prophylaxis.
Comorbidity phenotypes and risk of mortality in patients with ischaemic heart disease in the UK

Objectives The objective of this study is to use latent class analysis of up to 20 comorbidities in patients with a diagnosis of ischaemic heart disease (IHD) to identify clusters of comorbidities and to examine the associations between these clusters and mortality. Methods Longitudinal analysis of electronic health records in the health improvement network (THIN), a UK primary care database including 92 186 men and women aged ≥18 years with IHD and a median of 2 (IQR 1–3) comorbidities. Results Latent class analysis revealed five clusters with half categorised as a low-burden comorbidity group. After a median follow-up of 3.2 (IQR 1.4–5.8) years, 17 645 patients died. Compared with the low-burden comorbidity group, two groups of patients with a high-burden of comorbidities had the highest adjusted HR for mortality: those with vascular and musculoskeletal conditions, HR 2.38 (95% CI 2.28 to 2.49) and those with respiratory and musculoskeletal conditions, HR 2.62 (95% CI 2.45 to 2.79). Hazards of mortality in two other groups of patients characterised by cardiometabolic and mental health comorbidities were also higher than the low-burden comorbidity group; HR 1.46 (95% CI 1.39 to 1.52) and 1.55 (95% CI 1.46 to 1.64), respectively. Conclusions This analysis has identified five distinct comorbidity clusters in patients with IHD that were differentially associated with risk of mortality. These analyses should be replicated in other large datasets, and this may help shape the development of future interventions or health services that take into account the impact of these comorbidity clusters.
The fate of subsolid nodule: predictable or unpredictable?

J Thorac Dis 2020;12(3):1118-1120 | http://dx.doi. org/10.21037/jtd.2020. 01.56 We thank Dr. Hwang et al. from Korea for their thoughtful comments on our recent article (1). Our group recently published a study in the Heart, Lung and Circulation, reporting that natural course of persistent subsolid nodules (SSNs) in the term of true growth, substantial growth and stage shift (2). In this retrospective single-center study, we enrolled 128 persistent SSNs [93 pure ground-glass nodules (GGNs) and 35 part-solid nodules (PSNs)] 3 cm or smaller from 128 participants, and reported their observation during long-term follow-ups (average of 3.57 years, maximum follow-up period of 12 years). In this study, pure GGNs exhibited median natural progression times of 7, 9, and 12 years for true growth, substantial growth, and stage shift, respectively. However, PSNs exhibited median natural progression times of 3, 3, and 9 years for true growth, substantial growth, and stage shift, respectively. In recent years, increasing utilization of low-dose computed tomography (CT) tools for lung cancer screening has resulted in a significant portion of SSNs detected in the screening population, especially in an Asian population with the high prevalence of non-smoking related lung cancer (3,4). Overall, most patients (approx. 70%) with lung adenocarcinoma were non-smokers in Taiwan, and most patients with clinical symptoms are diagnosed at an advanced stage with poor survival outcome despite recent developments in the target therapy of late stage lung cancer over recent years (5). In the meantime, recent studies demonstrated that almost over 90% of screened lung cancers were stage I lung cancer (mostly adenocarcinoma spectrums) with the excellent prognosis in the Asian nonsmoking related lung cancer screening programs (3,6). The difference between non-screened and screened non-smoking related lung cancers make us notice the heterogeneity in growth pattern and risk factors of lung cancer (7). According to recent literature reviews, there were high prevalence of non-smoking related lung cancers found in the Asian lung cancer screening program (3,4,6). Most patients with lung cancers diagnosed in the screening program were not eligible for National Lung Screening Trial (NLST) criteria (3,8). Previous studies have demonstrated that family history and female gender are two important risk factors with non-smoking related lung cancer (3,4). In addition, the average PM2.5 levels in Taiwan were much higher than the levels in European cities according to the European Study of Cohorts for Air pollution Effects (ESCAPE), especially in Kaohsiung city (9). This issue of expanding screening eligibility criteria may be relevant in Taiwan because of regional genetic and environmental differences. Should never-smokers at increased risk for lung cancer be screened? Recent microsimulation study by Kelvin demonstrated that never-smokers with high risk (RRs of 15 to 35) have similar to better trade-offs between benefits and harms compared with population eligible for Letter to the Editor
Modeling Adaptation Strategies against Climate Change Impacts in Integrated Rice-Wheat Agricultural Production System of Pakistan

There are numerous anticipated effects of climate change (CC) on agriculture in the developing and the developed world. Pakistan is among the top ten most prone nations to CC in the world. The objective of this analysis was to quantify the economic impacts of CC on the agricultural production system and to quantify the impacts of suggested adaptation strategies at the farm level. The study was conducted in the Punjab province’s rice-wheat cropping system. For this purpose, climate modeling was carried out by using two representative concentration pathways (RCPs), i.e., RCPs 4.5 and 8.5, and five global circulation models (GCMs). The crop modeling was carried out by using the Agricultural Production Systems Simulator (APSIM) and the Decision Support System for Agrotechnology Transfer (DSSAT) crop simulation models (CSMs), which were tested on the cross-sectional data of 217 farm households collected from the seven strata in the study area. The socio-economic impacts were calculated using the Multidimensional Impact Assessment Tradeoff Analysis Model (TOA-MD). The results revealed that CC’s net economic impact using both RCPs and CSMs was negative. In both CSMs, the poverty status was higher in RCP 8.5 than in RCP 4.5. The adaptation package showed positive results in poverty reduction and improvement in the livelihood conditions of the agricultural households. The adoption rate for DSSAT was about 78%, and for APSIM, it was about 68%. The adaptation benefits observed in DSSAT were higher than in APSIM. The results showed that the suggested adaptations could have a significant impact on the resilience of the atmospheric changes. Therefore, without these adaptation measures, i.e., increase in sowing density, improved cultivars, increase in nitrogen use, and fertigation, there would be negative impacts of CC that would capitalize on livelihood and food security in the study area.
Protein-Bound Uremic Toxins in Hemodialysis Patients Relate to Residual Kidney Function, Are Not Influenced by Convective Transport, and Do Not Relate to Outcome

Protein-bound uremic toxins (PBUTs) are predominantly excreted by renal tubular secretion and hardly removed by traditional hemodialysis (HD). Accumulation of PBUTs is proposed to contribute to the increased morbidity and mortality of patients with end-stage kidney disease (ESKD). Preserved PBUT excretion in patients with residual kidney function (RKF) and/or increased PBUT clearance with improved dialysis techniques might improve the prognosis of patients with ESKD. The aims of this study are to explore determinants of PBUTs in HD patients, and investigate whether hemodiafiltration (HDF) lowers PBUT plasma concentrations, and whether PBUTs are related to the outcome. Predialysis total plasma concentrations of kynurenine, kynurenic acid, indoxyl sulfate, indole-3-acetic acid, p-cresyl sulfate, p-cresyl glucuronide, and hippuric acid were measured by UHPLC-MS at baseline and after 6 months of follow-up in the first 80 patients participating in the CONvective TRAnsport Study (CONTRAST), a randomized controlled trial that compared the effects of online HDF versus low-flux HD on all-cause mortality and new cardiovascular events. RKF was inversely related to kynurenic acid (p < 0.001), indoxyl sulfate (p = 0.001), indole-3-acetic acid (p = 0.024), p-cresyl glucuronide (p = 0.004) and hippuric acid (p < 0.001) plasma concentrations. Only indoxyl sulfate decreased by 8.0% (−15.3 to 34.6) in patients treated with HDF and increased by 11.9% (−15.4 to 31.9) in HD patients after 6 months of follow-up (HDF vs. HD: p = 0.045). No independent associations were found between PBUT plasma concentrations and either risk of all-cause mortality or new cardiovascular events. In summary, in the current population, RKF is an important determinant of PBUT plasma concentrations in HD patients. The addition of convective transport did not consistently decrease PBUT plasma concentrations and no relation was found between PBUTs and cardiovascular endpoints.
Perioperative Mortality and Long-Term Survival after Radical Cystectomy: A Population-Based Study in a Southern European Country on 4,389 Patients

Purpose: Population-based data on survival after radical cystectomy (RC) are lacking from Southern Europe. The aim of this study was to assess trends and determinants of perioperative mortality and long-term survival in the Veneto region (Northeastern Italy). Methods: All patients submitted to RC for bladder cancer from January 2004 to December 2016 were identified from the regional archive of hospital discharge records. Age at surgery, gender, comorbidities, hospital volume, calendar period of surgery, and type of urinary diversion were retrieved; vital status and cause of death were obtained by linkage with mortality records. Determinants of 90-day mortality were assessed by multilevel logistic regression; long-term survival was investigated by the Kaplan-Meier method and Cox regression. Results: Among 4,389 included patients, an increase in the share of patients aged ≥80 years (from 13% in 2004–2008 to 24% in 2013–2016, p < 0.001) and a decline in performing continent diversion (from 34.9 to 23.4%, p < 0.001) were observed across the study period. Ninety-day mortality did not change over time and was 4% for patients aged <70 years and 13.7% for those aged ≥80 years. Age- and comorbidities-adjusted mortality was significantly lower in hospitals performing >30 RCs/year (odds ratio 0.67, 95% confidence interval 0.48–0.93). At a median follow-up of 67 months, overall survival at 1 year and 5 years was 72 and 40%, respectively, with a higher rate among younger patients treated in high-volume hospitals. Conclusion: The population of patients treated with RC is rapidly ageing, with a high risk of perioperative and long-term mortality; this changing epidemiological scenario and better outcomes observed in high-volume hospitals support regionalization of the procedure.
Selenium and Salt Interactions in Black Gram (Vigna mungo L.): Ion Uptake, Antioxidant Defense System, and Photochemistry Efficiency

Salinity is a major abiotic stress which limits crop production, especially under rainfed conditions. Selenium (Se), as an important micronutrient, plays a vital role in mitigating detrimental effects of different abiotic stresses. The objective of this research was to examine the effect of Se fertilization on black gram (Vigna mungo) under salt stress. Our results showed that salt stress (100 mM NaCl) in leaves significantly induced oxidative damage and caused a decline in relative water content, chlorophyll (Chl), stomatal conductance (gs), photochemical efficiency (Fv/Fm), sucrose, and reducing sugars. A low dose of Se (1.5 ppm) significantly reduced hydrogen peroxide content, malondialdehyde formation, cell membrane damage, and also improved antioxidative enzyme activities, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, and glutathione peroxidase under salt stress. Se-treated plants exhibited higher Chl, gs, Fv/Fm, sucrose, and reducing sugars than untreated plants in response to salt stress. In addition, Se application enhanced Se uptake and reduced Na+ uptake, but Cl− remained unaffected. Our results indicated that a low dose of Se effectively alleviated salt damage via inhibition of Na+ uptake and enhanced antioxidant defense resulting in a significant decrease in oxidative damage, and maintained gaseous exchange and PS II function for sucrose and reducing sugars accumulation in black gram.