Miracle-dz/UniFAR-FTUs huggingface dataset:
Context‐dependent responses of food‐hoarding to competitors in Apodemus peninsulae: implications for coexistence among asymmetrical species

Abstract Superior species may have distinct advantages over subordinates within asymmetrical interactions among sympatric animals. However, exactly how the subordinate species coexists with superior species is unknown. In the forests west of Beijing City, intense asymmetrical interactions of food competition exist among granivorous rodents (e.g. Apodemus peninsulae, Niviventer confucianus, Sciurotamias davidianus and Tscherskia triton) that have broadly overlapping habitats and diets but have varied body size (range 15–300 g), hoarding habits (scatter vs larder) and/or daily rhythm (diurnal vs nocturnal). The smallest rodent, A. peninsulae, which typically faces high competitive pressure from larger rodents, is an ideal model to explore how subordinate species coexist with superior species. Under semi‐natural enclosure conditions, we tested responses of seed‐hoarding behavior in A. peninsulae to intraspecific and interspecific competitors in the situations of pre‐competition (without competitor), competition (with competitor) and post‐competition (competitor removed). The results showed that for A. peninsulae, the intensity of larder‐hoarding increased and the intensity of scatter‐hoarding declined in the presence of intraspecifics and S. davidianus, whereas A. peninsulae ceased foraging and hoarding in the presence of N. confucianus and T. triton. A. peninsulae reduced intensity of hoarding outside the nest and moved more seeds into the nest for larder‐hoarding under competition from intraspecific individuals and S. davidianus. In most cases, the experimental animals could recover to their original state of pre‐competition when competitors were removed. These results suggest that subordinate species contextually regulate their food‐hoarding strategies according to different competitors, promoting species coexistence among sympatric animals that have asymmetrical food competition.
Brief Intimate Partner Violence Perpetration Screening Tools: A Scoping Review

Recent research findings indicate that patients are willing to disclose their use of violence to health-care providers if asked. Health-care providers have a unique opportunity to screen their patients for intimate partner violence (IPV) perpetration; however, given the time constraints and limited personnel within medical offices and emergency-care facilities, instrument brevity is critically important. The development and evaluation of tools to screen for IPV perpetration in health-care settings, particularly brief instruments, is limited by the lack of adequate guidelines, clear institutional policies for screening, and reviews of the available literature. Given the need for validated measurement tools, we assessed the psychometric properties of measurement tools designed to quickly detect IPV perpetration by conducting a scoping review. Our search identified five measures meeting eligibility requirements. Inclusion criteria required that study information be published in a peer-reviewed journal, be published in English or Spanish languages, contain 10 or less items, report psychometric testing results, require no additional information, and be designed to detect IPV perpetration. We searched subject-specific databases and the bibliographies of relevant publications to identify studies. As part of appraising and synthesizing the evidence, we found most measures to have good reliability and validity. Most measures contained 2–5 items. Most studies were conducted in the United States and utilized an adequate sample size. There were considerable differences in how each of the measures determined a positive or negative screening. Gaps in the literature and areas for future research pertained to sample diversity, invariance testing, and practice guidelines for implementation.
The plant-type phosphoenolpyruvate carboxylase Gmppc2 is developmentally induced in immature soy seeds at the late maturation stage: a potential protein biomarker for seed chemical composition

ABSTRACT Phosphoenolpyruvate carboxylase (PEPC) is a carbon-fixing enzyme with critical roles in seed development. Previously we observed a positive correlation between PEPC activity and protein content in mature seeds among soybean cultivars and varietal differences of PEPC activity in immature seeds, which is concordant with seed protein accumulation. Here, we report a PEPC isoform (Gmppc2) which is preferentially expressed in immature soybean seeds at the late maturation stage. Gmppc2 was co-expressed with enzyme genes involved in starch degradation: α-amylase, hexokinase, and α-glucan phosphorylase. Gmppc2 was developmentally induced in the external seed coats, internal seed coats, hypocotyls, and cotyledons at the late maturation stage. The expression of Gmppc2 protein was negatively regulated by the application of a nitrogen fertilizer, which suppressed nodule formation. These results imply that Gmppc2 is involved in the metabolism of nitrogen originated from nodules into seeds, and Gmppc2 might be applicable as a biomarker of seed protein content. Abbreviations: PEP: phosphoenolpyruvate; PEPC: phosphoenolpyruvate carboxylase; RNA-Seq: RNA sequencing; PCA: principal component analysis; SE: standard error GRAPHICAL ABSTRACT A hypothetical model which represents the metabolism in Immature soybean seeds at the late maturation stage. In this model, PEPC (Gmppc2) acts on provision of oxaloacetate for biosynthesis of amino acids that were coordinately originated from vegetative organs.
Glucose Transport and Transporters in the Endomembranes

Glucose is a basic nutrient in most of the creatures; its transport through biological membranes is an absolute requirement of life. This role is fulfilled by glucose transporters, mediating the transport of glucose by facilitated diffusion or by secondary active transport. GLUT (glucose transporter) or SLC2A (Solute carrier 2A) families represent the main glucose transporters in mammalian cells, originally described as plasma membrane transporters. Glucose transport through intracellular membranes has not been elucidated yet; however, glucose is formed in the lumen of various organelles. The glucose-6-phosphatase system catalyzing the last common step of gluconeogenesis and glycogenolysis generates glucose within the lumen of the endoplasmic reticulum. Posttranslational processing of the oligosaccharide moiety of glycoproteins also results in intraluminal glucose formation in the endoplasmic reticulum (ER) and Golgi. Autophagic degradation of polysaccharides, glycoproteins, and glycolipids leads to glucose accumulation in lysosomes. Despite the obvious necessity, the mechanism of glucose transport and the molecular nature of mediating proteins in the endomembranes have been hardly elucidated for the last few years. However, recent studies revealed the intracellular localization and functional features of some glucose transporters; the aim of the present paper was to summarize the collected knowledge.
Circadian misalignment imposed by nocturnal feeding tends to increase fat deposition in pigs

Abstract Misalignment of day/night and feeding rhythms has been shown to increase fat deposition and the risk for metabolic disorders in humans and rodents. In most studies, however, food intake and intake patterns are not controlled. We studied the effects of circadian misalignment on energy expenditure in pigs while controlling for food intake as well as intake patterns. Twelve groups of five male pigs were housed in respiration chambers and fed either during the day (10.00–18.00 hours; DF) or night (22.00–06.00 hours; NF), bihourly the same sequential meals, representing 15, 10, 25, 30 and 20 % of the daily allowance. Paired feeding was applied to ensure equal gross energy intake between treatments. Apparent total tract digestibility, energy balances and heat partitioning were measured and analysed using a mixed linear model. Apparent total tract energy and DM digestibility tended to be lower for NF-pigs than DF-pigs (P < 0·10). Heat production was 3 % lower for NF-pigs than DF-pigs (P < 0·026), increasing fat retention by 7 % in NF-pigs (P = 0·050). NF-pigs were less active than DF-pigs during the feeding period, but more active during the fasting period. RMR was greater for DF-pigs than NF-pigs during the fasting period. Methane production was 30 % greater in NF-pigs than DF-pigs (P < 0·001). In conclusion, circadian misalignment has little effect on nutrient digestion, but alters nutrient partitioning, ultimately increasing fat deposition. The causality of the association between circadian misalignment and methane production rates remains to be investigated.
Psychometric Equivalence of the Computerized and Original Halstead Category Test Using a Matched Archival Sample

Objective: Investigate the equivalence of several psychometric measures between the traditional Halstead Category Test (HCT–Original Version [OV]) and the computer-based Halstead Category Test (HCT–Computerized Version [CV]). Method: Data were from a diagnostically heterogeneous, archival sample of 211 adults administered either the HCT by computer (n = 105) or cabinet (n = 106) as part of a neuropsychological evaluation. Groups were matched on gender, race, education, Full Scale Intelligence Quotient, and Global Neuropsychological Deficit Score. Confirmatory factor analysis was used to examine structural equivalence. Score, variability, and reliability equivalency were also examined. Differential item and test functioning under a Rasch model were examined. Results: An identified factor structure from research of the HCT-OV fit the HCT-CV scores adequately: χ2(4) = 8.83, p = . 07; root mean square error of approximation = 0.10 [0.00, 0.20]; standardized root mean residual = 0.03; comparative fit index = 0.99. Total scores and variability of subtest scores were not consistently equivalent between the two administration groups. Reliability estimates were, however, similar and adequate for clinical practice: 0.96 for HCT-OV and 0.97 for HCT-CV. About 17% of items showed possible differential item functioning, though just three of these items were statistically significant. Differential test functioning revealed expected total score differences of <1% between versions. Conclusion: The results of this study suggest that the HCT-CV functions similar to the HCT-OV with there being negligible differences in expected total scores between these versions. The HCT-CV demonstrated good psychometric properties, particularly reliability and construct validity consistent with previous literature. Further study is needed to generalize these findings and to further examine the equivalency of validity evidence between versions.
Thiol-based direct threat sensing by the stress-activated protein kinase Hog1

Arsenic directly stimulates the kinase Hog1 to promote the gene expression required for arsenic detoxification in yeast. Hog1 directly senses arsenic The mitogen-activated protein kinase (MAPK) Hog1 mediates the response of yeast cells to various stressors, including osmotic shock, heat shock, and arsenic. The arsenic-binding transcription factor Yap8 stimulates the expression of genes required for arsenic detoxification. Guerra-Moreno et al. found that arsenic bound directly to Hog1 through three conserved cysteine residues that were required for growth in the presence of arsenic but not for the response to osmotic stress. Arsenic binding to Hog1 promoted the phosphorylation and nuclear localization of Yap8 and enhanced the expression of Yap8 target genes. Thus, Hog1 directly senses arsenic to enhance Yap8-mediated arsenic detoxification. Some human MAPKs bound to arsenic in vitro, suggesting that arsenic binding by this family of kinases may be evolutionarily conserved. The yeast stress-activated protein kinase Hog1 is best known for its role in mediating the response to osmotic stress, but it is also activated by various mechanistically distinct environmental stressors, including heat shock, endoplasmic reticulum stress, and arsenic. In the osmotic stress response, the signal is sensed upstream and relayed to Hog1 through a kinase cascade. Here, we identified a mode of Hog1 function whereby Hog1 senses arsenic through a direct physical interaction that requires three conserved cysteine residues located adjacent to the catalytic loop. These residues were essential for Hog1-mediated protection against arsenic, were dispensable for the response to osmotic stress, and promoted the nuclear localization of Hog1 upon exposure of cells to arsenic. Hog1 promoted arsenic detoxification by stimulating phosphorylation of the transcription factor Yap8, promoting Yap8 nuclear localization, and stimulating the transcription of the only known Yap8 targets, ARR2 and ARR3, both of which encode proteins that promote arsenic efflux. The related human kinases ERK1 and ERK2 also bound to arsenic in vitro, suggesting that this may be a conserved feature of some members of the mitogen-activated protein kinase (MAPK) family. These data provide a mechanistic basis for understanding how stress-activated kinases can sense distinct threats and perform highly specific adaptive responses.
Mortality, morbidity, and cardiac surgery in Injection Drug Use (IDU)-associated versus non-IDU infective endocarditis: The need to expand substance use disorder treatment and harm reduction services

Background The addiction crisis is widespread, and unsafe injection practices among people who inject drugs (PWID) can lead to infective endocarditis. Methods A retrospective analysis of adult patients with definite or possible infective endocarditis admitted to a tertiary care center in Portland, Maine was performed over three-year period. Our primary objective was to examine differences in demographics, health characteristics, and health service utilization between injection drug use (IDU)-associated infective endocarditis and non-IDU infective endocarditis. The association between IDU and mortality, morbidity (defined as emergency department visits within 3 months of discharge), and cardiac surgery was examined. Bivariate and multivariate analyses were performed. A subgroup descriptive analysis of PWID was also performed to better examine substance use disorder (SUD) characteristics, treatment with medication for opioid use disorder (MOUD) and health service utilization. Results One-hundred and seven patients were included in the study, of which 39.2% (n = 42) had IDU-associated infective endocarditis. PWID were more likely to be homeless, uninsured, and lack a primary care provider. PWID were notably younger and had less documented comorbidities, however had similar in-hospital mortality rates (10% vs. 14%, p = 0.30), ED visits (50% vs. 54%, p = 0.70) and cardiac surgery (33% vs. 26%, p = 0.42) compared to those with non-IDU infective endocarditis. Ninety-day mortality was less among PWID (19.0% vs. 36.9%, p = 0.05). IDU was not associated with morbidity (adjusted odds ratio (AOR) 0.73, 95% CI 0.18–3.36), 90-day mortality (AOR 0.72, 95% CI 0.17–3.01), or cardiac surgery (AOR 0.15, 95% CI 0.03–0.69). Ninety-day mortality among PWID who received MOUD was lower (3% vs 15%, p = 0.45), as were ED visits (10% vs. 41%, p = 0.42) compared to those who did not receive MOUD. Conclusions Our results highlight existing differences in health characteristics and social determinants of health in people with IDU-associated versus non-IDU infective endocarditis. PWID had less comorbidities and were significantly younger than those with non-IDU infective endocarditis and yet still had similar rates of cardiac surgery, ED visits, and in-hospital mortality. These findings emphasize the need to deliver comprehensive health services, particularly MOUD and other harm reduction services, to this marginalized population.
Metal Cation Detection in Drinking Water

Maintaining a clean water supply is of utmost importance for human civilization. Human activities are putting an increasing strain on Earth’s freshwater reserves and on the quality of available water on Earth. To ensure cleanliness and potability of water, sensors are required to monitor various water quality parameters in surface, ground, drinking, process, and waste water. One set of parameters with high importance is the presence of cations. Some cations can play a beneficial role in human biology, and others have detrimental effects. In this review, various lab-based and field-based methods of cation detection are discussed, and the uses of these methods for the monitoring of water are investigated for their selectivity and sensitivity. The cations chosen were barium, cadmium, chromium, copper, hardness (calcium, magnesium), lead, mercury, nickel, silver, uranium, and zinc. The methods investigated range from optical (absorbance/fluorescence) to electrical (potentiometry, voltammetry, chemiresistivity), mechanical (quartz crystal microbalance), and spectrometric (mass spectrometry). Emphasis is placed on recent developments in mobile sensing technologies, including for integration into microfluidics.
Emerging role of secreted miR-210-3p as potential biomarker for clear cell Renal Cell Carcinoma metastasis.

BACKGROUND MicroRNAs (miRNAs) are emerging as promising molecules in the diagnosis, prognosis and treatment of urological tumours. Recently, our group performed two independent studies highlighting that miR-210-3p may be a useful biomarker not only for diagnosis but also for post-surgery clear cell Renal Cell Carcinoma (ccRCC) management. OBJECTIVE The aim of this study is to further explore the effectiveness of miRNA as non-invasive biomarker for clinical outcomes and ccRCC response to the treatment. METHODS We analyzed miR-210-3p levels in neoplastic and healthy tissue and in urine specimens collected at surgery and during follow-up of 21 ccRCC patients by RTpPCR. RESULTS Firstly, we confirmed that the expression of miR-210-3p was upregulated in tumor tissues and in urine samples of analyzed cohort. Of note is that miR-210-3p expression was significantly reduced in urine samples from disease-free patients during follow-up (from 3 to 12 months) compared to the baseline levels observed at the time of surgery. In a small subgroup of patients presenting metastatic progression (such as bone, intestinal or lung metastasis), the urine levels of miR-210-3p correlated with responsiveness to the therapy. CONCLUSIONS This pilot study highlights the relevance of secreted miR-210-3p as powerful non-invasive prognostic and predictive biomarker for the evaluation of clinical outcomes and treatment response during ccRCC follow up.
Real time dynamics of Gating-Related conformational changes in CorA

CorA, a divalent-selective channel in the metal ion transport superfamily, is the major Mg2+-influx pathway in prokaryotes. CorA structures in closed (Mg2+-bound), and open (Mg2+-free) states, together with functional data showed that Mg2+-influx inhibits further Mg2+-uptake completing a regulatory feedback loop. While the closed state structure is a symmetric pentamer, the open state displayed unexpected asymmetric architectures. Using high-speed atomic force microscopy (HS-AFM), we explored the Mg2+-dependent gating transition of single CorA channels: HS-AFM movies during Mg2+-depletion experiments revealed the channel’s transition from a stable Mg2+-bound state over a highly mobile and dynamic state with fluctuating subunits to asymmetric structures with varying degree of protrusion heights from the membrane. Our data shows that at Mg2+-concentration below Kd, CorA adopts a dynamic (putatively open) state of multiple conformations that imply structural rearrangements through hinge-bending in TM1. We discuss how these structural dynamics define the functional behavior of this ligand-dependent channel.
Public practices on antibiotic use: A cross-sectional study among Qatar University students and their family members

Background Antimicrobial resistance (AMR) is a serious public health problem and a global concern. The inappropriate use of antibiotics has been identified by the World Health Organization as a major risk factor for AMR. Methods The purpose of this research study is to assess the prevalence of inappropriate antibiotic use among Qatar University students and their family members, detect sociodemographic factors associated with inappropriate use, evaluate the knowledge and attitude towards antibiotic use, and assess respondents’ opinions on healthcare providers’ antibiotic prescription practices. Participants (N = 596) completed a self-administered questionnaire. Descriptive analysis, the Pearson chi-squared test, and multivariate logistic regression analyses were performed. Results The major inappropriate antibiotic use practices followed by the respondents were using antibiotics without prescription (82%), not completing the antibiotic course (45%), and obtaining antibiotics from the pharmacy without prescription (23%). The chi-square test results showed that age (p = 0.031) and nationality (p = 0.041) were associated with using antibiotics without prescription. In addition, respondents less than 21 years of age (p<0.001), who had only a secondary education (p = 0.007), and who lived in one of the large and crowded cities in Qatar (p = 0.011) had higher odds of stopping the antibiotic before completing the course. Our study also revealed that almost 60% of the respondents had inadequate knowledge and a negative attitude towards antibiotic use. Nationality and municipality were the independent factors associated with having appropriate knowledge of antibiotic use. Univariate logistic regression analyses in our study demonstrated that older (>26 years), married and university-graduated participants were more likely to have a positive attitude towards antibiotic use than others. Respondents also reported that neither doctors nor pharmacists were providing adequate patient education about appropriate antibiotic use. The Socio-Ecological Model was applied to interpret the findings and frame implications. Conclusion The findings shed light on various factors shaping antibiotic use practices and provide evidence to design multilevel behavioral interventions to improve public practices of antibiotic use.
Lenalidomide, p53 and del(5q) Myelodysplastic Syndrome: Ribosome Stress Relief

Whereas deletions involving the long arm of chromosome 5 are among the most common chromosomal abnormalities in myelodysplastic syndrome (MDS), isolated del(5q) MDS, which includes the 5q- syndrome, is rare and characterized by hypoplastic anemia and a moderate risk of transformation to acute myeloid leukemia (AML). The 5q- syndrome is now recognized as a ribosomopathy, and both the classic 5q- syndrome and del(5q) MDS are uniquely responsive to lenalidomide. However, the mechanism of action of lenalidomide is controversial and involves modulation of p53 activity, which may be beneficial in anemia remission but suggested to lead to malignant cell outgrowth. Here, we critically review the literature on this important controversy, which has obvious implications for therapy of del (5q) MDS.