Miracle-dz/UniFAR-FTUs huggingface dataset:
Multi-Autoantibody Signature and Clinical Outcome in Membranous Nephropathy.

BACKGROUND AND OBJECTIVES Patients with membranous nephropathy can have circulating autoantibodies against membrane-bound (phospholipase A2 receptor 1 [PLA2R1] and thrombospondin type-1 domain containing 7A[THSD7A]) and intracellular (aldose reductase, SOD2, and α-enolase) podocyte autoantigens. We studied their combined association with clinical outcomes. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS Serum levels of anti-PLA2R1, anti-THSD7A, anti-aldose reductase, anti-SOD2, and anti-α-enolase autoantibodies were determined in 285 patients at diagnosis and during follow-up using standardized and homemade assays. An eGFR>60 ml/min per 1.73 m2 and remission of proteinuria (<0.3/<3.5 g per d) after 12 months were the outcomes of interest. RESULTS At diagnosis, 182 (64%), eight (3%), and 95 (33%) patients were anti-PLA2R1+, anti-THSD7A+, and double negative, respectively. The prevalence of a detectable antibody to at least one intracellular antigen was similarly distributed in patients who were anti-PLA2R1+ (n=118, 65%) and double negative (n=64, 67%). Positivity for anti-PLA2R1, anti-SOD2, and anti-α-enolase antibodies and higher titers at diagnosis were associated with poor clinical outcome independently to each other. Combined positivity for anti-PLA2R1, anti-SOD2, and anti-α-enolase was associated with highest risk of poor outcome (odds ratio, 5.5; 95% confidence interval, 1.2 to 24; P=0.01). In Kaplan-Meier analysis, patients who were anti-PLA2R1+/anti-SOD2+ or anti-PLA2R1+/anti-α-enolase+ had lower eGFR at 12 months compared with patients who were anti-PLA2R1+/anti-SOD2- or anti-α-enolase-. Predictive tests (net reclassification index and area under the curve-receiver-operating characteristic analysis) showed that combined assessment of antibodies improved classification of outcome in 22%-34% of cases for partial remission of proteinuria and maintenance of normal eGFR. For patients with nephrotic syndrome at diagnosis, anti-SOD2 positivity and high anti-PLA2R1 titer were associated with a lack of complete remission. Patients who were anti-PLA2R1-/anti-intracellular antigens- had the lowest proteinuria and the highest eGFR at diagnosis and the lowest risk of lower eGFR at 12 months. Epitope spreading was present in 81% of patients who were anti-PLA2R1+ and was associated with increased positivity for intracellular antigens and poor eGFR at diagnosis and 12 months. CONCLUSIONS Combined serological analysis of autoantibodies targeting membrane-bound and intracellular autoantigens identifies patients with poor clinical outcomes.
The Fungicidal Action of Micafungin is Independent on Both Oxidative Stress Generation and HOG Pathway Signaling in Candida albicans

In fungi, the Mitogen-Activated Protein kinase (MAPK) pathways sense a wide variety of environmental stimuli, leading to cell adaptation and survival. The HOG pathway plays an essential role in the pathobiology of Candida albicans, including the colonization of the gastrointestinal tract in a mouse model, virulence, and response to stress. Here, we examined the role of Hog1 in the C. albicans response to the clinically relevant antifungal Micafungin (MF), whose minimum inhibitory concentration (MIC) was identical in the parental strain (RM100) and in the isogenic homozygous mutant hog1 (0.016 mg/L). The cell viability was impaired without significant differences between the parental strain, the isogenic hog1 mutant, and the Hog1+ reintegrant. This phenotype was quite similar in a collection of hog1 mutants constructed in a different C. albicans background. MF-treated cells failed to induce a relevant increase of both reactive oxygen species (ROS) formation and activation of the mitochondrial membrane potential in parental and hog1 cells. MF was also unable to trigger any significant activation of the genes coding for the antioxidant activities catalase (CAT1) and superoxide dismutase (SOD2), as well as on the corresponding enzymatic activities, whereas a clear induction was observed in the presence of Amphotericin B (AMB), introduced as a positive control of Hog1 signaling. Furthermore, Hog1 was not phosphorylated by the addition of MF, but, notably, this echinocandin caused Mkc1 phosphorylation. Our results strongly suggest that the toxic effect of MF on C. albicans cells is not mediated by the Hog1 MAPK and is independent of the generation of an internal oxidative stress in C. albicans.
Co-Circulation of Two Independent Clades and Persistence of CHIKV-ECSA Genotype during Epidemic Waves in Rio de Janeiro, Southeast Brazil

The Chikungunya virus infection in Brazil has raised several concerns due to the rapid dissemination of the virus and its association with several clinical complications. Nevertheless, there is limited information about the genomic epidemiology of CHIKV circulating in Brazil from surveillance studies. Thus, to better understand its dispersion dynamics in Rio de Janeiro (RJ), one of the most affected states during the 2016–2019 epidemic waves, we generated 23 near-complete genomes of CHIKV isolates from two main cities located in the metropolitan mesoregion, obtained directly from clinical samples. Our phylogenetic reconstructions suggest the 2019-CHIKV-ECSA epidemic in RJ state was characterized by the co-circulation of multiple clade (clade A and B), highlighting that two independent introduction events of CHIKV-ECSA into RJ state have occurred between 2016–2019, both mediated from the northeastern region. Interestingly, we identified that the two-clade displaying eighteen characteristic amino acids changes among structural and non-structural proteins. Our findings reinforce that genomic data can provide information about virus genetic diversity and transmission dynamics, which might assist in the arbovirus epidemics establishing of an effective surveillance framework.
Self-Assembly of Antiferromagnetically-Coupled Copper(II) Supramolecular Architectures with Diverse Structural Complexities

The self-assembly of 2,6-diformyl-4-methylphenol (DFMP) and 1-amino-2-propanol (AP)/2-amino-1,3-propanediol (APD) in the presence of copper(II) ions results in the formation of six new supramolecular architectures containing two versatile double Schiff base ligands (H3L and H5L1) with one-, two-, or three-dimensional structures involving diverse nuclearities: tetranuclear [Cu4(HL2−)2(N3)4]·4CH3OH·56H2O (1) and [Cu4(L3−)2(OH)2(H2O)2] (2), dinuclear [Cu2(H3L12−)(N3)(H2O)(NO3)] (3), polynuclear {[Cu2(H3L12−)(H2O)(BF4)(N3)]·H2O}n (4), heptanuclear [Cu7(H3L12−)2(O)2(C6H5CO2)6]·6CH3OH·44H2O (5), and decanuclear [Cu10(H3L12−)4(O)2(OH)2(C6H5CO2)4] (C6H5CO2)2·20H2O (6). X-ray studies have revealed that the basic building block in 1, 3, and 4 is comprised of two copper centers bridged through one μ-phenolate oxygen atom from HL2− or H3L12−, and one μ-1,1-azido (N3−) ion and in 2, 5, and 6 by μ-phenoxide oxygen of L3− or H3L12− and μ-O2− or μ3-O2− ions. H-bonding involving coordinated/uncoordinated hydroxy groups of the ligands generates fascinating supramolecular architectures with 1D-single chains (1 and 6), 2D-sheets (3), and 3D-structures (4). In 5, benzoate ions display four different coordination modes, which, in our opinion, is unprecedented and constitutes a new discovery. In 1, 3, and 5, Cu(II) ions in [Cu2] units are antiferromagnetically coupled, with J ranging from −177 to −278 cm−1.
Does solvation cause symmetry breaking in the I3− ion in aqueous solution?

We seek to answer the question posed in the title by simulation of the tri-iodide ion in water, modeling the intermolecular interactions by classical potentials. The decrease in solvation free energy as a function of the dipole moment of the ion is calculated using an extended dynamics simulation method. This decrease is approximately quadratic in the ion dipole. Symmetry breaking occurs if this decrease is greater than the energy required to polarize the ion. We use ab initio calculations on an isolated ion to find the electronic and vibrational contributions to the polarizability, from which the polarization energy can be calculated. The solvated ion is found to be more stable when displaced along the asymmetric stretching coordinate, due to contributions of this deformation to the molecular dipole. As a test of the model’s reliability, it is used to derive solvation force autocorrelation functions from which time scales for vibrational energy and phase relaxation are estimated. The results are demonstr.
Detection of breastmilk antibodies targeting SARS-CoV-2 nucleocapsid, spike and receptor-binding-domain antigens

ABSTRACT A 40-year-old female was found to have strongly neutralizing SARS-CoV-2 breastmilk IgA and IgG antibodies reactive against multiple SARS-CoV-2 antigens at 2.5 months after documented infection with SARS-CoV-2. At 6.5 months following the infection, she remained positive for breastmilk and serum SARS-CoV-2 neutralizing antibodies. Holder breast milk pasteurization did not diminish SARS-CoV-2 antibody titres but it reduced its neutralizing capacity, while serum heat inactivation had no negative effect on SARS-CoV-2 serum antibody levels and neutralizing capacity. Current data on SARS-CoV-2 and breastmilk are reviewed.
The Mask of Suicide

Abstract Although it has been stated that the majority of suicidal people give definite warnings of their suicidal intention, a percentage of suicidal people may dissemble (or mask), possibly 20%. The aim of this psychological autopsy (PA) study was to explore the mask of suicide, examining age and sex of the decedent, and survivors’ relationship to the deceased. A PA study in Norway, with 120 survivors/informants, was undertaken. Overall, 80% of informants reported manifest and/or latent content of deception (dissembling); well above the 20% suggested. Three main themes emerged from the interviews of the 95 survivors that were related to the mask. In the opinion of the bereaved, reasons for the mask were due to: 1) Inability to adjust/impairment; 2) Relational problems; and 3) Weakened resilience. Differences in masking or (self) deception were found in the age of the decedent, but not in sex, nor in the survivors’ closeness of the relationship. Older deceased people were perceived to exhibit more dissembling, associated to the suicide. Limitations are noted in this beginning study into the mask of suicide, and it is concluded that much greater research is needed to unmask the dangerous dissembling, maybe in some, self-deception.
The role of personality in the thoughts, feelings, and behaviors of students in Germany during the first weeks of the COVID-19 pandemic

We examined how the thoughts, feelings, and behaviors of 6,957 students from Germany, assessed between March 16 and April 21, 2020, when COVID-19 became a serious health concern in Germany, varied by personality. The Big Five personality traits—openness to experience, conscientiousness, extraversion, agreeableness, and emotional stability—were assessed with the International Personality Item Pool. Students were asked whether they kept up with the COVID-19 news, followed specific governmental rules and recommendations (washing hands more, using public transport less, avoiding larger crowds, and restricting meetings with family/friends), hoarded supplies, felt less secure in public places, or expected financial losses due to the crisis. Logistic regressions adjusted for sociodemographic factors and cognitive abilities revealed that more conscientious (odds ratio (OR) = 1.133) and more agreeable (OR = 1.285) students kept up with the news more. More agreeable students were also more likely to wash their hands more often/intensively (OR = 1.262), use public transport less (OR = 1.182), avoid crowds (OR = 1.320), and restrict meetings with family/friends (OR = 1.410). Other Big Five traits were not associated with these behaviors, except that less emotionally stable individuals tended to use public transport less often (OR = 1.162). Additionally, less emotionally stable students, in particular, more often bought more supplies than usual (OR = 1.322), felt insecure in public spaces (OR = 1.597), and expected financial losses (OR = 1.270). Moreover, less open (OR = 0.876) and more conscientious (OR = 1.235) students more often felt insecure in public spaces, and more extraverted individuals more often expected financial losses (OR = 1.180). Taken together, our findings suggest that more agreeable individuals, in particular, tend to comply with governmental rules and recommendations to fight COVID-19, whereas less emotionally stable individuals, in particular, tend to hoard supplies, feel insecure, and fear financial losses due to the crisis.
Intermolecular Interactions in Crystal Structures of Imatinib-Containing Compounds

Imatinib, one of the most used therapeutic agents to treat leukemia, is an inhibitor that specifically blocks the activity of tyrosine kinases. The molecule of imatinib is flexible and contains several functional groups able to take part in H-bonding and hydrophobic interactions. Analysis of molecular conformations for this drug was carried out using density functional theory calculations of rotation potentials along single bonds and by analyzing crystal structures of imatinib-containing compounds taken from the Cambridge Structural Database and the Protein Data Bank. Rotation along the N-C bond in the region of the amide group was found to be the reason for two relatively stable molecular conformations, an extended and a folded one. The role of various types of intermolecular interactions in stabilization of the particular molecular conformation was studied in terms of (i) the likelihood of H-bond formation, and (ii) their contribution to the Voronoi molecular surface. It is shown that experimentally observed hydrogen bonds are in accord with the likelihood of their formation. The number of H-bonds in ligand-receptor complexes surpasses that in imatinib salts due to the large number of donors and acceptors of H-bonding within the binding pocket of tyrosine kinases. Contribution of hydrophilic intermolecular interactions to the Voronoi molecular surface is similar for both conformations, while π. π stacking is more typical for the folded conformation of imatinib.
Changes in Season Affect Body Weight, Physical Activity, Food Intake, and Sleep in Female College Students: A Preliminary Study

The current study examined how body weight and lifestyle fluctuate between spring, autumn, and winter in Japanese female college students and whether weight gain is associated with changes in physical activity, food intake, and sleep. We measured body weight and lifestyle factors in 31 participants from May 2017 to January 2018. Weight was measured daily in participants’ homes. Physical activity and sleep were measured for three weeks in three seasons using two accelerometers. Food intake was assessed using a validated food frequency questionnaire. Body weight significantly decreased in autumn compared with spring (p < 0.001). Body weight in winter tended to increase compared with autumn (p = 0.052). Step counts and energy intake were significantly different between seasons (p < 0.05). Total time in bed was not significantly different between seasons. In comparisons of changes in lifestyle patterns from autumn to winter between the weight gain (≥0.5 kg) and weight maintenance groups, seasonal changes in lifestyle factors were not significantly different between groups (p > 0.05). The results indicated that body weight and lifestyle were affected by seasonal variability in female college students, but no significant relationships existed between seasonal weight gain and changes in lifestyle patterns.
Responses of Pigs to Stunning with Nitrogen Filled High-Expansion Foam

Simple Summary Stunning pigs with carbon dioxide gas is one of the most common methods for commercial slaughter. Carbon dioxide, however, has been shown to be aversive for pigs and causes a high degree of distress before they lose consciousness. Stunning with nitrogen gas is less aversive than with carbon dioxide, and an innovative method that delivers the nitrogen gas in high-expansion foam in a closed container could potentially improve pig welfare at stunning. Pigs were exposed to either air-filled foam, nitrogen-filled foam, or no foam in air, and the behavioural and physiological responses were assessed. The pigs did not show any strong aversive behaviours when exposed to foam, regardless of whether it was air-filled or nitrogen-filled foam. However, they seemed to avoid putting their heads and snouts into the foam, and the rate of escape attempts through the lid increased when foam levels became high. Five minutes after the nitrogen foam production started, the pigs were assessed to be in deep unconsciousness or dead. Based on the results found, stunning with the nitrogen foam technique may be a viable alternative to carbon dioxide stunning and offer improved animal welfare. Further studies are needed to assess the new method for stunning of slaughter-weight pigs. Abstract Nitrogen gas (N2) delivered in high expansion foam in a closed container could be a feasible method for humanely stunning pigs. This study aimed to evaluate potential aversion in pigs to the N2 foam method and its effect on stun quality. Furthermore, the study aimed to assess potential aversion to the foam itself. Sixty pigs (27.8 ± 4.4 kg) were divided into three treatments and were exposed to either N2-filled foam, air-filled foam, or no foam air. The N2 foam was effective at purging the air from the container and quickly created stable anoxic conditions. The pigs did not show any strong aversive behaviours when exposed to foam. However, they seemed to avoid putting their heads and snouts into the foam when foam levels became high. Escape attempts through the lid also increased when the foam started covering their heads. The mean time to loss of posture was 57.9 s. Based on the results, stunning with the N2 foam technique could be a viable alternative to high concentration CO2 stunning and potentially lead to improved animal welfare at slaughter.
Expression of Hybrid Peptide EF-1 in Pichia pastoris, Its Purification, and Antimicrobial Characterization

EF-1 is a novel peptide derived from two bacteriocins, plantaricin E and plantaricin F. It has a strong antibacterial activity against Escherichia coli and with negligible hemolytic effect on red blood cells. However, the chemical synthesis of EF-1 is limited by its high cost. In this study, we established a heterologous expression of EF-1 in Pichia pastoris. The transgenic strain successfully expressed hybrid EF-1 peptide, which had a molecular weight of ~5 kDa as expected. The recombinant EF-1 was purified by Ni2+ affinity chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), which achieved a yield of 32.65 mg/L with a purity of 94.9%. The purified EF-1 exhibited strong antimicrobial and bactericidal activities against both Gram-positive and -negative bacteria. Furthermore, propidium iodide staining and scanning electron microscopy revealed that EF-1 can directly induce cell membrane permeabilization of E. coli. Therefore, the hybrid EF-1 not only preserves the individual properties of the parent peptides, but also acquires the ability to disrupt Gram-negative bacterial membrane. Meanwhile, such an expression system can reduce both the time and cost for large-scale peptide production, which ensures its potential application at the industrial level.
Anthocyanin Pigments: Beyond Aesthetics

Anthocyanins are polyphenol compounds that render various hues of pink, red, purple, and blue in flowers, vegetables, and fruits. Anthocyanins also play significant roles in plant propagation, ecophysiology, and plant defense mechanisms. Structurally, anthocyanins are anthocyanidins modified by sugars and acyl acids. Anthocyanin colors are susceptible to pH, light, temperatures, and metal ions. The stability of anthocyanins is controlled by various factors, including inter and intramolecular complexations. Chromatographic and spectrometric methods have been extensively used for the extraction, isolation, and identification of anthocyanins. Anthocyanins play a major role in the pharmaceutical; nutraceutical; and food coloring, flavoring, and preserving industries. Research in these areas has not satisfied the urge for natural and sustainable colors and supplemental products. The lability of anthocyanins under various formulated conditions is the primary reason for this delay. New gene editing technologies to modify anthocyanin structures in vivo and the structural modification of anthocyanin via semi-synthetic methods offer new opportunities in this area. This review focusses on the biogenetics of anthocyanins; their colors, structural modifications, and stability; their various applications in human health and welfare; and advances in the field.
Progress in Modern Marine Biomaterials Research

The growing demand for new, sophisticated, multifunctional materials has brought natural structural composites into focus, since they underwent a substantial optimization during long evolutionary selection pressure and adaptation processes. Marine biological materials are the most important sources of both inspiration for biomimetics and of raw materials for practical applications in technology and biomedicine. The use of marine natural products as multifunctional biomaterials is currently undergoing a renaissance in the modern materials science. The diversity of marine biomaterials, their forms and fields of application are highlighted in this review. We will discuss the challenges, solutions, and future directions of modern marine biomaterialogy using a thorough analysis of scientific sources over the past ten years.
Differences in Illness Severity among Circulating Norovirus Genotypes in a Large Pediatric Cohort with Acute Gastroenteritis

Norovirus is a major pathogen identified in children with acute gastroenteritis (AGE), little is known about the strain’s diversity and their clinical severity. Stool and/or rectal swabs were collected from children ≤18 years of age recruited at emergency departments (ED), and a provincial nursing advice phone line due to AGE symptoms in the province of Alberta, Canada between December 2014 and August 2018. Specimens were tested using a reverse transcription real time PCR and genotyped by Sanger sequencing. The Modified Vesikari Scale score (MVS) was used to evaluate the disease severity. The objectives are to identify the Genogroup and Genotype distribution and to compare illness severity between the GI and GII genogroups and to complete further analyses comparing the GII genotypes identified. GII.4 was the genotype most commonly identified. Children with GII.4 had higher MVS scores (12.0 (10.0, 14.0; p = 0.002)) and more prolonged diarrheal (5 days (3.0, 7.8)) and vomiting (3.2 days (1.7, 5.3; p < 0.001)) durations compared to other non GII.4 strains. The predominant strain varied by year with GII.4 Sydney[P31] predominant in 2014/15, GII.4 Sydney[P16] in 2015/16 and 2017/18, and GII.3[P12] in 2016/17. Genogroup II norovirus strains predominated in children with AGE with variance between years; clinical severity associated with different strains varied with episodes being most severe among GII.4 infected children.