Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of rescue oral glucocorticoid therapy during inpatient cystic fibrosis exacerbations

An acute pulmonary exacerbation (APE) in cystic fibrosis (CF) is characterized by increased pulmonary symptoms attributed to bacterial colonization, neutrophil recruitment, and inflammation. Antimicrobials, airway clearance, and nutrition are the mainstay of therapy. However, when patients fail to improve, corticosteroids have been added to therapy. We retrospectively examined the use of rescue steroids in a children's hospital from 2013 to 2017 during CF APE treatment following at least 1 week of inpatient therapy without expected clinical improvement. In total, 106 encounters of 53 unique patients, aged 6–20 years, who had FEV1 percent predicted (FEV1pp) data at baseline, admission, midpoint, and discharge, and had admission duration of at least 12 days were studied. Encounters treated with steroids had less improvement at midpoint percent change from admission in FEV1pp (4.9 ± 11.3) than nonsteroid group change in FEV1pp (20.1 ± 24.6; p < .001). Failure to improve as expected was the rationale for steroid use. At discharge, there was no difference in mean FEV1pp (p = .76). Delays in steroid therapy by waiting until the end of the second week increased the total length of stay (LOS). Propensity matching, comparing outcomes in patients without midpoint improvement in FEV1pp, was also evaluated. There was no difference in admission or discharge FEV1pp between groups. Equally, no difference in FEV1pp at follow‐up visit or in time until the next APE was detected. Secondary analysis for associations including gender, genotype, fungal colonization, or inhaled antimicrobials was nonsignificant. These data suggest rescue use of corticosteroids during APE does not predictably impact important outcome measures during CF APE treatment.
Gui-A-Gra Attenuates Testicular Dysfunction in Varicocele-Induced Rats via Oxidative Stress, ER Stress and Mitochondrial Apoptosis Pathway

Gui-A-Gra, a commercial insect powder from Gryllus bimaculatus, is registered as an edible insect by the Korean food and drug administration. The aim of this study was to investigate the effect of Gui-A-Gra on testicular damage induced by experimental left varicocele in male Sprague Dawley rats. A total of 72 rats were randomly divided into the following six groups (12 rats in each group): a normal control group (CTR), a group administrated with Gui-A-Gra 1.63 gm/kg (G1.63), a group administrated with Gui-A-Gra 6.5 gm/kg (G6.5), a varicocele (VC)-induced control group (VC), a VC-induced group administrated with Gui-A-Gra 1.63 gm/kg (VC + G1.63), and a VC-induced group administrated with Gui-A-Gra 6.5 gm/kg (VC + G6.5). Rats were administrated 1.63 or 6.5 gm/kg Gui-A-Gra once daily for 42 days. Indicators of sperm parameters, histopathology, reproductive hormones, oxidative stress, endoplasmic reticulum (ER) stress, inflammation, and mitochondrial apoptosis were analyzed to evaluate effects of Gui-A-Gra on VC-induced testicular dysfunction. Gui-A-Gra administration to VC-induced rats significantly (p < 0.05) increased sperm count and sperm motility, Johnsen score, spermatogenic cell density, serum testosterone, testicular superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, GPx4, and steroidogenic acute regulatory protein (StAR) level. Moreover, pretreatment with Gui-A-Gra significantly (p < 0.05) decreased terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) positive cells/tubules, serum luteinizing hormone (LH), serum follicle-stimulating hormone (FSH), testicular tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA), reactive oxygen species (ROS)/reactive nitrogen species (RNS) level, glucose-regulated protein-78 (Grp-78), phosphorylated c-Jun-N-terminal kinase (p-JNK), phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1α (p-IRE1α), cleaved caspase-3, and BCL2 associated X protein: B-cell lymphoma 2 (Bax: Bcl2) ratio in VC rats. These results suggest that protective effects of Gui-A-Gra on VC-induced testicular injury might be due to its antioxidant, anti-inflammatory, and androgenic activities that might be mediated via crosstalk of oxidative stress, ER stress, and mitochondrial apoptosis pathway.
Poziom akceptacji zmian na rynku żywności pochodzenia zwierzęcego w opinii konsumentów

S t r e s z c z e n i e Konsumenci w Polsce przejawiają niski poziom innowacyjności wyboru zywności, kupując produkty zywnościowe znane i spelniające zroznicowane indywidualne oczekiwania. Tym samym mozna domniemywac, ze poziom akceptacji innowacyjnych produktow zywnościowych wśrod konsumentow jest niski. Celem potwierdzenia tego przypuszczenia przeprowadzono badania terenowe w ramach projektu: ”BIOŻYWNOŚC – innowacyjne, funkcjonalne produkty pochodzenia zwierzecego”. Badania o charakterze ilościowym, typu omnibus, przeprowadzono w czerwcu 2011 roku na 1000-osobowej ogolnopolskiej probie badawczej. Na podstawie uzyskanych wynikow stwierdzono, ze polscy konsumenci zauwazają zmiany na rynku zywności w Polsce, w ostatnich latach, jakkolwiek nie wszystkie spośrod nich oceniają korzystnie, a tym samym akceptują je w zroznicowanym stopniu. W odniesieniu do zywności pochodzenia zwierzecego badani konsumenci w najwyzszym stopniu akceptowali zmiany polegające na zmniejszeniu zawartości niektorych skladnikow np. soli czy cukru, natomiast najnizszy stopien akceptacji deklarowano wobec wzbogacania mleka i jego przetworow witaminami i skladnikami mineralnymi. Wyniki badan potwierdzily tez, ze wyzszy poziom akceptacji wiekszości zmian w zywności pochodzenia zwierzecego deklarowali konsumenci mlodsi, o wyzszych dochodach, legitymujący sie wyzszym wyksztalcenia oraz pochodzący z wiekszych miast. Slowa kluczowe: konsument, akceptacja zmian w zywności pochodzenia zwierzecego, innowacje, innowacyjnośc
Integration of the Transcriptome and Genome-Wide Landscape of BRD2 and BRD4 Binding Motifs Identifies Key Superenhancer Genes and Reveals the Mechanism of Bet Inhibitor Action in Rheumatoid Arthritis Synovial Fibroblasts

Key Points BET inhibitor JQ1 downregulated BRD2/BRD4 superenhancer genes in RA-FLS. JQ1 inhibited key inflammatory pathways in activated RA-FLS. JQ1 altered the chromatin occupancy of proinflammatory transcription factors. Fibroblast-like synoviocytes (FLS), one of the main cell types of the rheumatoid arthritis (RA) synovium, possess phenotypic and molecular characteristics of transformed cells. JQ1, an inhibitor of the bromodomain and extra terminal domain family that includes BRD2, BRD3, BRD4, and BRDt, has shown efficacy in models of arthritis. We demonstrate that the active isomer of JQ1 but not its inactive isomer inhibits IL-1β–induced RA-FLS activation and proliferation. To understand the mechanism of JQ1 action, we subjected JQ1-treated RA-FLS to transcriptional profiling and determined BRD2 and BRD4 cistromes by identifying their global chromatin binding sites. In addition, assay for transposable accessible chromatin by high throughput sequencing was employed to identify open and closed regions of chromatin in JQ1-treated RA-FLS. Through an integrated analysis of expression profiling, Brd2/Brd4 cistrome data, and changes in chromatin accessibility, we found that JQ1 inhibited key BRD2/BRD4 superenhancer genes, downregulated multiple crucial inflammatory pathways, and altered the genome-wide occupancy of critical transcription factors involved in inflammatory signaling. Our results suggest a pleiotropic effect of JQ1 on pathways that have shown to be individually efficacious in RA (in vitro, in vivo, and/or in humans) and provide a strong rationale for targeting BRD2/BRD4 for disease treatment and interception.
The torsional conformations of butane: Definitive energetics from ab initio methods

The torsional potential function for butane was probed theoretically using increasingly complete basis sets (up to 840 functions) and treatments of electron correlation [up to the CCSD(T) method] until it was shown that the sequence of relative energies approached convergence. The Schrodinger limit in the Born–Oppenheimer approximation was thus estimated. The equilibrium energies relative to the anti conformation (ΔEe) as obtained by this focal-point extrapolation were 0.62, 3.31, and 5.51 kcal mol−1 for the geometrically optimized stationary points having carbon backbone torsion angles of 64.8°, 119.6°, and 0°, respectively. The final prediction of the anti–syn difference is 5.40±0.15 kcal mol−1. Some consequences of this result are discussed.
The peptide vaccine of the future.

The approach of peptide-based anti-cancer vaccination has proven the ability to induce cancer-specific immune responses in multiple studies for various cancer entities. However, clinical responses remain so far limited to single patients and broad clinical applicability was not achieved. Therefore, further efforts are required to improve peptide vaccination in order to integrate this low side effect therapy into the clinical routine of cancer therapy. To design clinically effective peptide vaccines in the future, different issues have to be addressed and optimized comprising antigen target selection as well as choice of optimal adjuvants and vaccination schedules. Furthermore, the combination of peptide-based vaccines with other immuno- and molecular targeted therapies as well as the development of predictive biomarkers could further improve efficacy. In this review, current approaches in the development of peptide-based vaccines and critical implications for optimal vaccine design are discussed.
Gastrointestinal Strongyles Egg Excretion in Relation to Age, Gender, and Management of Horses in Italy

Simple Summary Horses worldwide are plagued by gastrointestinal parasites that can lead to severe health problems. The occurrence and intensity of these worm infections vary based on the geographical location, season, and animal management strategies applied. One of these strategies is to monitor the parasite situation in different parts of the world. This research investigates the abundance, proportions, and risk factors of strongyle egg shedding of horses in Italy. Overall, the results showed that approximately 40% of all horses in Italy shed strongyle eggs and that almost 90% of stables have at least one infected animal. In addition, most parasite eggs are found in just a small fraction of the horse population, confirming the need for improved parasite control strategies. Abstract Current equine helminth control strategies play a key role in strongyle epidemiology and anthelmintic resistance and have led to the recommendation for new treatment plans, which include diagnostic and efficacy surveillance. Assessing the equine strongyle distribution patterns would thus be useful and this study describes the strongyle prevalence in the equine population in Italy through coprological analysis and coproculture. In addition, individual data on each animal were collected in order to identify risk factors associated with strongyle egg shedding. Of the total number of stables investigated, 86.4% were found to have at least one positive animal and a 39.5% prevalence of strongyle egg shedding with a mean eggs per gram (EPG) of 245. A total of 80% of the total recorded EPG was shed from 12.8% of positive horses, thus confirming the need for new targeted intervention strategies. Significant differences in parasite prevalence were found based on season, sex, geographical distribution, management and rearing system, and breed. Significantly lower EPG values were found in horses that had received anthelmintic treatment, and macrocyclic lactones (MLs) were the most effective. Lastly, although large strongyles are more pathogenic, horses in Italy are mainly burdened by small strongyles, which pose an important animal health risk requiring continuous parasitological monitoring.
Cutting forces in wood machining – Basics and applications in industrial processes. A review COST Action E35 2004–2008: Wood machining – micromechanics and fracture

Abstract The data available in the literature concerning wood cutting forces permits to build models or to simulate the main wood machining processes (milling, sawing, peeling, etc.). This approach contributes to a better understanding of formation of wood surfaces and chips and the data may be helpful to optimise cutting geometry, reduce tool wear, improve tool material, and to size tool-machines. The models may also be useful for industrial application in two ways: (1) providing data to optimise the settings for a given operation (batch approach), and (2) building predictive models that could be the basis of an online control system for the machining processes (interactive approach). A prerequisite for this is that numerous machining tests on different wood materials are performed based on experiences with different kind of tools and experimental devices. With a focus on potential industrial applications, the emphasis of this review was on the wood peeling process, which is a very demanding special case of wood cutting. Although not so many industrial machines are equipped with expensive force sensors, there is a lot of high quality information available about cutting forces which may be useful to improve the scientific or technological knowledge in wood machining. Alternative parameters, such as vibration or sound measurements, appear to be promising substitutes in the praxis, particularly to feed online control systems of any wood cutting process.
Local and Contralateral Effects after the Application of Neuromuscular Electrostimulation in Lower Limbs

Neuromuscular electrostimulation (NMES) has been used mainly as a method to promote muscle strength, but its effects on improving blood flow are less well known. The aim of this study is to deepen the knowledge about the local and contralateral effects of the application of symmetric biphasic square currents on skin temperature (Tsk). An experimental pilot study was developed with a single study group consisting of 45 healthy subjects. Thermographic evaluations were recorded following the application of NMES to the anterior region of the thigh. The results showed an increase in the maximal Tsk of 0.67% in the anterior region of the thigh where the NMES was applied (p < 0.001) and an increase of 0.54% (p < 0.01) due to cross-education effects, which was higher when the NMES was applied on the dominant side (0.79%; p < 0.01). The duration of the effect was 20 min in the dominant leg and 10 min in the nondominant one. The application of a symmetrical biphasic current (8 Hz and 400 μs) creates an increase in the maximal Tsk at the local level. A temperature cross-education effect is produced, which is greater when the NMES is applied on the dominant side. This could be a useful noninvasive measurement tool in NMES treatments.
Qualitative Review of Clinical Guidelines for Medical and Surgical Management of Urolithiasis: Consensus and Controversy 2020.

INTRODUCTION Many major guidelines across the globe address the medical and surgical management of urolithiasis. We elected to compare and contrast the recommendations among the five most highly-cited guidelines on stone disease to offer insights on where evidence has created a consensus and where there remains ongoing controversy and hence a need for the pursuit of studies that will provide a higher level of evidence. METHODS We reviewed the American Urological Association (AUA) 2019 medical and 2016 surgical guidelines, the 2016 Canadian Urological Association (CUA) guidelines, the 2020 European Association of Urology (EAU) guidelines, the 2019 National Institute of Health and Care Excellence (NICE), and the 2019 Urological Association of Asia (UAA). Tables correlating guideline statements by topic were created, and a comparative analysis was conducted to ascertain consensus and discordance. RESULTS Comparative analysis of recommendations from the AUA guidelines to the CUA, EAU, NICE guidelines, and UAA revealed a high consensus surrounding the medical management of stones. In terms of the surgical management of stones, there is high consensus regarding the treatment of ureteral stones including medical expulsive therapy using alpha blockers, not pre-stinting for uncomplicated ureteroscopy, and employment of either ureteroscopy or shockwave lithotripsy (SWL) as first line treatment. There is high consensus among the AUA, EAU, NICE, and UAA guidelines regarding renal stone treatment; the CUA does not have guidelines on the management of renal stones. Unlike the AUA and NICE, the CUA and EAU make specific recommendations regarding selection of patients for SWL procedures, including stone density, skin-to-stone distance, treatment rate, acoustic coupling, and post-SWL use of medical expulsive therapy. CONCLUSIONS There are many areas of consensus and only minor areas of conflict among the most up-to-date AUA, CUA, EAU, NICE, and UAA guidelines on the medical and surgical management of stone disease. Conflicts among guidelines and areas of low evidence, such as follow-up imaging strategies and stone surveillance, the use of an ureteral access sheath in ureteroscopy, and guidance on the use of miniaturized percutaneous nephrolithotomy, are opportunities for novel, impactful high grade clinical studies.
Miniaturized Salting-Out Assisted Liquid-Liquid Extraction Combined with Disposable Pipette Extraction for Fast Sample Preparation of Neonicotinoid Pesticides in Bee Pollen

As the main source of nutrients for the important pollinator honeybee, bee pollen is crucial for the health of the honeybee and the agro-ecosystem. In the present study, a new sample preparation procedure has been developed for the determination of neonicotinoid pesticides in bee pollen. The neonicotinoid pesticides were extracted using miniaturized salting-out assisted liquid-liquid extraction (mini-SALLE), followed by disposable pipette extraction (DPX) for the clean-up of analytes. Effects of DPX parameters on the clean-up performance were systematically investigated, including sorbent types (PSA, C18, and silica gel), mass of sorbent, loading modes, and elution conditions. In addition, the clean-up effect of classical dispersive solid-phase extraction (d-SPE) was compared with that of the DPX method. Results indicated that PSA-based DPX showed excellent clean-up ability for the high performance liquid chromatography (HPLC) analysis of neonicotinoid pesticides in bee pollen. The proposed DPX method was fully validated and demonstrated to provide the advantage of simple and rapid clean-up with low consumption of solvent. This is the first report of DPX method applied in bee pollen matrix, and would be valuable for the development of a fast sample preparation method for this challenging and important matrix.
Ordering of aluminum in tetrahedral sites in mixed-layer 2:1 phyllosilicates by solid-state high-resolution NMR

Al and Si MAS NMR results are presented for the mixed-layer 2:1 phyllosilicate rectorite. Separate Si spectra, obtained by partially relaxed inversion-recovery experiments, for the two types of tetrahedral sheets present are consistent with one sheet being smectite-like, i.e. low aluminum occupancy of tetrahedral sites, and the other sheet being mica-like, i.e. high aluminum occupancy of tetrahedral sites. Comparison of the relative areas of the Si(0-3Al) resonances from the mica-like sheets with those predicted by computer modeling indicates a high degree of ordering of substituted aluminum. The observed areas indicate that minimization of both Al-O-Al (Loewenstein's rule) and Al-O-Si-O-Al (Dempsey's rule) interactions is important. For the tetrahedral sheets Si/Al ratios derived from Al MAS spectra indicate a much higher aluminum occupancy than determined by XRF or Si NMR.
Effects of Oxytetracycline and Gentamicin Therapeutic Doses on Hematological, Biochemical and Hematopoietic Parameters in Cyprinus carpio Juveniles

Simple Summary Hematological, biochemical and hematopoietic effects of therapeutic doses of oxytetracycline (OTC) and gentamicin (GEN) in clinically healthy common carp juveniles were studied. The fish were divided into four groups: controls 1 and 2 (untreated or injected with 0.6% NaCl solution), and two groups treated with antibiotics (orally with OTC four times every two days or injected with a single dose of GEN dissolved in 0.6% NaCl). Blood and head kidneys were sampled from all fish 3 days post-treatments for hematological, biochemical and hematopoietic tissue analyses. The obtained results showed no considerable hematotoxicity or hepatotoxicity of therapeutic doses of OTC and GEN to carp. Abstract Hematological, biochemical and hematopoietic effects of therapeutic doses of two antibiotics, oxytetracycline (OTC) and gentamicin (GEN), in clinically healthy common carp juveniles were studied. The fish were divided into four groups: controls 1 and 2 (untreated or injected with 0.6% NaCl solution), and two groups treated with antibiotics (orally with 75 mg/kg OTC four times every two days or injected with a single dose (4 mg/kg) of GEN dissolved in 0.6% NaCl). Blood and head kidneys were sampled from all fish 3 days post-treatments for hematological, biochemical and hematopoietic tissue analyses. No major alterations in the values of hematological and serum biochemical parameters occurred following administration of OTC or GEN. Glucose concentrations were significantly lower in both groups of fish subjected to injections (Control 2 and GEN), while the oxidative metabolic activity of phagocytes increased in the antibiotic-treated groups (significantly in OTC). More alterations were observed in hematopoietic tissue. Immunocytochemical analysis revealed that G caused a significant increase in the rate of cell proliferation (PCNA-positive cells) and an increase in the frequency of apoptotic cells (caspase-positive). The frequency of lymphoid lineage decreased, which was related to a decrease in the abundance of mature lymphocytes in GEN-treated fish. Percentages of neutrophilic lineage were significantly elevated in OTC and GEN groups compared to controls. The obtained results showed no considerable hematotoxicity or hepatotoxicity of therapeutic doses of OTC and GEN to carp.
Role of Cyclooxygenase-2 in Head and Neck Tumorigenesis

The cyclooxygenase-2 (COX-2) is a potent enzyme that converts arachidonic acid to prostaglandins (PG), including PGE2, a key mediator of inflammation and angiogenesis. Importantly, COX-2 is activated in response to inflammatory stimuli, where it is also believed to promote the development and progression of head and neck cancers (HNC). COX-2 can mediate its protumorigenic effect through various mechanisms, such as inducing cell proliferation, inhibition of apoptosis, and suppressing the host’s immune response. Furthermore, COX-2 can induce the production of vascular endothelial growth factors, hence, promoting angiogenesis. Indeed, the ability of COX-2 inhibitors to selectively restrict the proliferation of tumor cells and mediating apoptosis provides promising therapeutic targets for cancer patients. Thus, in this comprehensive review, we summarized the reported differential expression patterns of COX-2 in different stages of head and neck carcinogenesis—from potentially premalignant lesions to invasive carcinomas. Furthermore, we examined the available meta-analysis evidence for COX-2 role in the carcinogenesis of HNC. Finally, further understanding of the biological processes of COX-2 and its role in orchestrating cell proliferation, apoptosis, and angiogenesis may give therapeutically beneficial insight to develop the management plan of HNC patients and improve their clinical outcomes.