Miracle-dz/UniFAR-FTUs huggingface dataset:
Two Cycle Learning: Clustering Based Regularisation for Deep Semi-Supervised Classification

This works addresses the challenge of classification with minimal annotations. Obtaining annotated data is time consuming, expensive and can require expert knowledge. As a result, there is an acceleration towards semi-supervised learning (SSL) approaches which utilise large amounts of unlabelled data to improve classification performance. The vast majority of SSL approaches have focused on implementing the \textit{low-density separation assumption}, in which the idea is that decision boundaries should lie in low density regions. However, they have implemented this assumption by treating the dataset as a set of individual attributes rather than as a global structure, which limits the overall performance of the classifier. Therefore, in this work, we go beyond this implementation and propose a novel SSL framework called two-cycle learning. For the first cycle, we use clustering based regularisation that allows for improved decision boundaries as well as features that generalises well. The second cycle is set as a graph based SSL that take advantages of the richer discriminative features of the first cycle to significantly boost the accuracy of generated pseudo-labels. We evaluate our two-cycle learning method extensively across multiple datasets, outperforming current approaches.
Overcoming the Intrinsic Gefitinib-resistance via Downregulation of AXL in Non-small Cell Lung Cancer

Background Resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), such as gefitinib, is a limited factor in the treatment of non-small-cell lung cancer (NSCLC) patients. Therefore, ongoing studies are trying to identify EGFR-TKIs-resistant mechanisms and to discover novel therapeutic strategies and targets for NSCLC treatment. Methods In the present study, the possibility of overcoming intrinsic gefitinib-resistance was examined by regulating the expression of AXL. A natural product-derived antitumor agent, yuanhuadine (YD) was employed to modulate the expression of AXL in the cells. Results Treatment with YD effectively downregulated AXL expression in AXL-overexpressed gefitinib-resistant H1299 cells. The combination of gefitinib and YD exhibited a synergistic grwoth-inhibitory activity in H1299 cells by downregulation of AXL expression. Conclusions Based on these findings, AXL was found to be a promising therapeutic target to overcome the intrinsic resistance to gefitinib in NSCLC. Furthermore, YD is able to effectively regulate the expression of AXL and thus it may be applicable as a potential lead compound for the treatment of gefitinib-resistant NSCLC.
Quantum Private Information Retrieval from MDS-coded and Colluding Servers

In the classical private information retrieval (PIR) setup, a user wants to retrieve a file from a database or a distributed storage system (DSS) without revealing the file identity to the servers holding the data. In the quantum PIR (QPIR) setting, a user privately retrieves a classical file by downloading quantum systems from the servers. The QPIR problem has been treated by Song et al. in the case of replicated servers, both without collusion and with all but one servers colluding. In this paper, the QPIR setting is extended to account for maximum distance separable (MDS) coded servers. The proposed protocol works for any [n, k]-MDS code and t-collusion with t = n − k. Similarly to the previous cases, the rates achieved are better than those known or conjectured in the classical counterparts.
DAPLE protein inhibits nucleotide exchange on Gαs and Gαq via the same motif that activates Gαi

Besides being regulated by G-protein–coupled receptors, the activity of heterotrimeric G proteins is modulated by many cytoplasmic proteins. GIV/Girdin and DAPLE (Dvl-associating protein with a high frequency of leucine) are the best-characterized members of a group of cytoplasmic regulators that contain a Gα-binding and -activating (GBA) motif and whose dysregulation underlies human diseases, including cancer and birth defects. GBA motif–containing proteins were originally reported to modulate G proteins by binding Gα subunits of the Gi/o family (Gαi) over other families (such as Gs, Gq/11, or G12/13), and promoting nucleotide exchange in vitro. However, some evidence suggests that this is not always the case, as phosphorylation of the GBA motif of GIV promotes its binding to Gαs and inhibits nucleotide exchange. The G-protein specificity of DAPLE and how it might affect nucleotide exchange on G proteins besides Gαi remain to be investigated. Here, we show that DAPLE's GBA motif, in addition to Gαi, binds efficiently to members of the Gs and Gq/11 families (Gαs and Gαq, respectively), but not of the G12/13 family (Gα12) in the absence of post-translational phosphorylation. We pinpointed Met-1669 as the residue in the GBA motif of DAPLE that diverges from that in GIV and enables better binding to Gαs and Gαq. Unlike the nucleotide-exchange acceleration observed for Gαi, DAPLE inhibited nucleotide exchange on Gαs and Gαq. These findings indicate that GBA motifs have versatility in their G-protein–modulating effect, i.e. they can bind to Gα subunits of different classes and either stimulate or inhibit nucleotide exchange depending on the G-protein subtype.
Policaptil Gel Retard Intake Reduces Postprandial Triglycerides, Ghrelin and Appetite in Obese Children: A Clinical Trial

The aim of this study is to test the hypothesis that the intake of Policaptil Gel Retard® (PGR) is able to affect appetite, metabolic and hormonal postprandial profile in obese children. 46 obese children were randomly assigned to treatment with PGR or placebo, in a double blind clinical trial. Two PGR tablets or placebo were given in fasting condition, before the ingestion of a mixed meal (15 kcal/kg lean body mass). Blood samples were taken at baseline and for 4 h, for measuring blood lipids, glucose, insulin, ghrelin, and glucagon like peptide-1 (GLP-1). Appetite was quantified using a visual analog scale. Children assuming PGR had a significantly lower increase of postprandial triglycerides (area under the curve (AUC): 3021 (2879) vs. 5038 (3738) mg × 240 min/Dl) and appetite (−234 (274) vs. 36 (329)) than children assuming placebo. The AUC of ghrelin was significantly lower after PGR ingestion, than after placebo (−8179 (8073) vs. −2800 (7579) pg × 240 min/mL). Blood glucose, insulin, non-esterified fatty acids (NEFA) and GLP-1 profiles were not significantly different in the two groups. In conclusion, a single intake of two tablets of PGR was associated with a significant reduction of appetite, ghrelin, and triglycerides in the postprandial period in obese children. Further investigation will assess if a chronic intake of PGR may affect body weight and glucose metabolism.
Assessing Amphiphilic ABAB Zn(II) Phthalocyanines with Enhanced Photosensitization Abilities in In Vitro Photodynamic Therapy Studies Against Cancer

We have previously demonstrated that singlet oxygen photosensitization abilities of Zn(II) phthalocyanines (Zn(II)Pcs) are enhanced through α-functionalization with bulky fluorinated substituents (i.e., bis(trifluoromethyl)phenyl units) at facing positions of ABAB Zn(II)Pcs, where A and B refer to differently functionalized isoindoles. In this work, we have prepared the Zn(II)Pc ABAB 1 endowed with hydrophilic triethylene glycol monomethyl ether (i.e., at the A isoindoles) to provide solubility in aqueous media, together with its A3B and A4 counterparts, and compared their ability to behave as photosensitizers for photodynamic therapy. All photophysical data, aggregation studies and preliminary in vitro biological assays in cell cultures of SCC-13 (squamous cell carcinoma) and HeLa (cervical cancer cells), have proved ABAB 1 as the best photosensitizer of the series.
Interactions of Paraoxonase-1 with Pharmacologically Relevant Carbamates

Mammalian paraoxonase-1 hydrolyses a very broad spectrum of esters such as certain drugs and xenobiotics. The aim of this study was to determine whether carbamates influence the activity of recombinant PON1 (rePON1). Carbamates were selected having a variety of applications: bambuterol and physostigmine are drugs, carbofuran is used as a pesticide, while Ro 02-0683 is diagnostic reagent. All the selected carbamates reduced the arylesterase activity of rePON1 towards the substrate S-phenyl thioacetate (PTA). Inhibition dissociation constants (Ki), evaluated by both discontinuous and continuous inhibition measurements (progress curves), were similar and in the mM range. The rePON1 displayed almost the same values of Ki constants for Ro 02-0683 and physostigmine while, for carbofuran and bambuterol, the values were approximately ten times lower and two times higher, respectively. The affinity of rePON1 towards the tested carbamates was about 3–40 times lower than that of PTA. Molecular modelling of rePON1-carbamate complexes suggested non-covalent interactions with residues of the rePON1 active site that could lead to competitive inhibition of its arylesterase activity. In conclusion, carbamates can reduce the level of PON1 activity, which should be kept in mind, especially in medical conditions characterized by reduced PON1 levels.
HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells

Simple Summary Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation and differentiation, but the function of HDAC4 in the skeletal muscle satellite cells (SMSCs) of chickens is still unknown. Here, we demonstrated that knockdown of HDAC4 inhibits the proliferation and differentiation of chicken SMSCs but has no significant effect on its apoptosis. These results suggest that HDAC4 has an essential role in skeletal muscle growth and in the development of chicken. Abstract The development of skeletal muscle satellite cells (SMSCs) is a complex process that could be regulated by many genes. Previous studies have shown that Histone Deacetylase 4 (HDAC4) plays a critical role in cell proliferation, differentiation, and apoptosis in mouse. However, the function of HDAC4 in chicken muscle development is still unknown. Given that chicken is a very important meat-producing animal that is also an ideal model to study skeletal muscle development, we explored the functions of HDAC4 in chicken SMSCs after the interference of HDAC4. The results showed that HDAC4 was enriched in embryonic skeletal muscle, and it was highly expressed in embryonic muscle than in postnatal muscles. Meanwhile, knockdown of HDAC4 could significantly inhibit the proliferation and differentiation of chicken SMSCs but had no effect on the apoptosis of SMSCs as observed in a series of experiment conducted in vitro. These results indicated that HDAC4 might play a positive role in chicken skeletal muscle growth and development.
Loneliness, Family Communication, and School Adjustment in a Sample of Cybervictimized Adolescents

The objective of this study was to compare individual, family, and social variables, such as the perception of loneliness, family communication, and school adjustment in a sample of 2399 Andalusian (Spanish) adolescents aged 12 to 18 (M = 14.63, SD = 1.91) suffering from cybervictimization (low, moderate, and high). The results show that adolescents suffering from high cybervictimization report more loneliness, more problematic communication with both parents, and worse school adjustment than the rest of the groups. Regarding gender, differences are observed in open communication with the mother and in the dimensions of school adjustment, being more favorable for girls. However, there were no significant differences between girls and boys in the loneliness variable. The interaction effects indicate, on the one hand, that female severe cybervictims present more avoidant communication with the mother than the other groups, and, on the other hand, that male cybervictims of all three groups and female severe cybervictims have lower academic competence than the group of female low cybervictims, followed by female moderate cybervictims. These data support the idea that, depending on its intensity and duration, cybervictimization affects girls and boys differently in terms of individual, family, and social variables.
OCTA characterisation of microvascular retinal alterations in patients with central serous chorioretinopathy

Purpose This study aimed to describe the characteristics of microvascular retinal alterations in eyes with chronic central serous chorioretinopathy (CSC) employing optical coherence tomography angiography (OCTA) analysis. Methods We collected data from 472 eyes with chronic CSC from 336 patients who had OCTA obtained. Each OCTA image was graded by two readers to assess the presence of microvascular retinal alterations, including regions of vascular rarefaction/retinal hypoperfusion, enlargement of the foveal avascular zone (FAZ) and presence of telangiectasias or microaneurysms. Volume spectral domain optical coherence tomography (SD-OCT) scans were obtained through the macula and the OCT was correlated with the OCTA findings in eyes with retinal vascular alterations. Results OCTA displayed microvascular retinal alterations in 18 out of 474 eyes (3.6%) from 14 patients (13 male and 1 female; mean±SD age was 54.7±11.1 years). One eye displayed the presence of retinal telangiectasias, while 17 out of 18 eyes were graded as having areas of retinal vascular rarefactions, and 3 out of 17 eyes were also characterised by an enlargement of the FAZ. The parafoveal region was the location most involved by retinal vascular changes (66,7%), followed by foveal (22,2%) and perifoveal (11.1%) regions, respectively. Conclusion Although CSC is known to represent a choroidal disorder, retinal vascular alterations may be present in these eyes and OCTA may represent a useful tool to identify and describe them.
Unsupervised Cross-Subject Adaptation for Predicting Human Locomotion Intent

Accurately predicting human locomotion intent is beneficial in controlling wearable robots and in assisting humans to walk smoothly on different terrains. Traditional methods for predicting human locomotion intent require collecting and labeling the human signals, and training specific classifiers for each new subject, which introduce a heavy burden on both the subject and the researcher. In addressing this issue, the present study liberates the subject and the researcher from labeling a large amount of data, by incorporating an unsupervised cross-subject adaptation method to predict the locomotion intent of a target subject whose signals are not labeled. The adaptation is realized by designing two classifiers to maximize the classification discrepancy and a feature generator to align the hidden features of the source and the target subjects to minimize the classification discrepancy. A neural network is trained by the labeled training set of source subjects and the unlabeled training set of target subjects. Then it is validated and tested on the validation set and the test set of target subjects. Experimental results in the leave-one-subject-out test indicate that the present method can classify the locomotion intent and activities of target subjects at the averaged accuracy of 93.60% and 94.59% on two public datasets. The present method increases the user-independence of the classifiers, but it has been evaluated only on the data of subjects without disabilities. The potential of the present method to predict the locomotion intent of subjects with disabilities and control the wearable robots will be evaluated in future work.
Patient preferences for maintenance therapy in Crohn’s disease: A discrete-choice experiment

Objective To quantify patient preferences for maintenance therapy of Crohn’s disease and understand the impact on treatment selection. Methods We conducted a discrete-choice experiment in patients with Crohn’s disease (n = 155) to measure the importance of attributes relevant to choosing between different medical therapies for maintenance of Crohn’s disease. The attributes included efficacy and withdrawals due to adverse events, as well as dosing and other rare risks of treatment. From the discrete-choice experiment we estimated the part-worth (importance) of each attribute level, and explored preference heterogeneity through latent class analysis. We then used the part-worths to apply weights across each outcome from a prior network meta-analysis to estimate patients’ preferred treatment in pairwise comparisons and for the overall group of treatments. Results The discrete-choice experiment revealed that maintaining remission was the most important attribute. Patients would accept a rare risk of infection or cancer for a 14% absolute increased chance of remission. Latent class analysis demonstrated that 45% of the cohort was risk averse, either to adverse events or requiring a course of prednisone. When these preferences were used in modelling studies to compare pairs of treatments, there was a ≥ 78% probability that all biologic treatments were preferred to azathioprine and methotrexate, based on the balance of benefits and harms. When comparing all treatments, adalimumab was preferred by 53% of patients, who were motivated by efficacy, and vedolizumab was preferred by 30% who were driven by the preference to avoid risks. However, amongst biologic treatment options, there was considerable uncertainty regarding the preferred treatment at the individual patient level. Conclusion Patients with Crohn’s disease from our population were, on average, focused on the benefits of treatment, supporting intensive treatment approaches aimed at maintaining remission. Important preference heterogeneity was identified, however, highlighting the importance of shared decision making when selecting treatments.
Conjunctival lymphangiectasia as a biomarker of severe systemic disease in Ser77Tyr hereditary transthyretin amyloidosis

Aims To investigate the relationship between the ophthalmic and systemic phenotypes in patients with hereditary transthyretin amyloidosis with the S77Y mutation (ATTRS77Y). Methods In this cross-sectional study, patients with genetically confirmed ATTRS77Y amyloidosis were enrolled. All patients underwent complete neurological examination, including staging with the Neuropathy Impairment Score (NIS), Polyneuropathy Disability (PND) score; complete cardiological evaluation, including echocardiography, cardiac MRI and/or cardiac scintigraphy and complete ophthalmic evaluation, including slit lamp examination and fundus examination. Ocular ancillary tests (fluorescein and indocyanine green angiography, and anterior segment optical coherence tomography) were performed in cases with abnormal findings. The Kruskal-Wallis test was used for quantitative outcomes and Fisher’s exact test for qualitative outcomes. Statistical significance was indicated by p<0.05 (two tailed). Results The study sample was composed of 24 ATTRS77Y patients. The mean patient age was 58.4±12.4 years. None of the patients presented with amyloid deposits in the anterior chamber, secondary glaucoma or vitreous amyloidosis. Retinal angiopathy was observed in four patients, complicated with retinal ischaemia in one patient. Conjunctival lymphangiectasia (CL) was detected in 13 patients (54%), associated with perilymphatic amyloid deposits. The presence of CL was statistically associated with more severe neurological disease (NIS=43.3±31.9 vs 18.9±20.4; PND=2.6±1.0 vs 1.4±0.7 in patients with and without CL, respectively; both p<0.05) and amyloid cardiomyopathy (p=0.002). Conclusion In ATTRS77Y patients, CL is common and could serve as a potential biomarker for severe systemic disease. There were neither anterior chamber deposits, secondary glaucoma nor vitreous deposits in ATTRS77Y patients.
The level of total immunoglobulin E as an indicator of disease grade in adults with severe atopic dermatitis.

Atopic dermatitis is one of the most common inflammatory dermatoses in children and adults. The disorder is associated with significant worsening of quality of life. Due to the frequently suggested pathogenetic relationship with atopic dermatitis, immunoglobulin E seems to be an adequate indicator of the disease. AIM The aim of the study was to assess the association between the total serum immunoglobulin E levels and the grade of skin lesions measured with the SCORAD and oSCORAD scales in the population of adult patients suffering from severe atopic dermatitis. MATERIALS AND METHODS The study was performed in the group of 31 patients suffering from atopic dermatitis since early childhood (17 women and 14 men) in 2017. Total IgE was measured by the fluoroenzymatic immunoassay. The Spearman's rank correlation coefficient was used to determine the power of the relationship between the concentration of total IgE and atopic dermatitis severity. The Kruskal-Wallis and Mann- Whitney's tests were used to compare the distribution of IgE concentration and the distribution of atopic dermatitis grade. RESULTS The statistically significant correlation between SCORAD/ oSCORAD and the measured IgE levels was found in the patients with the IgE level above 3500 IU/ml (19 subjects). CONCLUSIONS The present study demonstrated that in the patients with severe extrinsic atopic dermatitis, the concentration of total IgE was correlated with the severity of the disease. This suggests an opportunity of employing IgE as an atopic dermatitis biomarker.
A Spatio-Temporal Multi-Scale Binary Descriptor

Binary descriptors are widely used for multi-view matching and robotic navigation. However, their matching performance decreases considerably under severe scale and viewpoint changes in non-planar scenes. To overcome this problem, we propose to encode the varying appearance of selected 3D scene points tracked by a moving camera with compact spatio-temporal descriptors. To this end, we first track interest points and capture their temporal variations at multiple scales. Then, we validate feature tracks through 3D reconstruction and compress the temporal sequence of descriptors by encoding the most frequent and stable binary values. Finally, we determine multi-scale correspondences across views with a matching strategy that handles severe scale differences. The proposed spatio-temporal multi-scale approach is generic and can be used with a variety of binary descriptors. We show the effectiveness of the joint multi-scale extraction and temporal reduction through comparisons of different temporal reduction strategies and the application to several binary descriptors.
The Impact of Heat Waves on Emergency Department Visits in Roanoke, Virginia

Extreme heat, often coupled with high humidity, is a source of environmental stress to the human body. The resulting strain can lead to morbidity or mortality. Prior heat-related research has emphasized the impact of heat events on mortality, but there is a growing interest in understanding the influence of heat on emergency department (ED) visits, hospitalizations, and other disease metrics.