Miracle-dz/UniFAR-FTUs huggingface dataset:
Identiﬁcation of Motifs of Burkholderia pseudomallei BimA Required for Intracellular Motility, Actin Binding, and Actin Polymerization (cid:1)

Actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires BimA ( Burkholderia i ntracellular m otility A ). The mechanism by which BimA mediates actin assembly at the bacterial pole is ill-deﬁned. Toward an understanding of the regions of B. pseudomallei BimA required for intracellular motility and the binding and polymerization of actin, we constructed plasmid-borne bimA variants and glutathione- S transferase fusion proteins with in-frame deletions of speciﬁc motifs. A 13-amino-acid direct repeat and IP 7 proline-rich motif were dispensable for actin binding and assembly in vitro , and expression of the mutated proteins in a B. pseudomallei bimA mutant restored actin-based motility in J774.2 murine macrophage-like cells. However, two WASP homology 2 (WH2) domains were found to be required for actin binding, actin assembly, and plaque formation. A tract of ﬁve PDASX direct repeats inﬂuenced the polymerization of pyrene-actin monomers in vitro and was required for actin-based motility and intercellular spread, but not actin binding. None of the mutations impaired surface expression or polar targeting of BimA. The number of PDASX repeats varied in natural isolates from two to seven. Such repeats acted additively to promote pyrene-actin polymerization in vitro , with stepwise increases in the rate of polymerization as the number of repeats was increased. No differences in the efﬁciency of actin tail formation could be discerned between strains expressing BimA variants with two, ﬁve, or seven PDASX repeats. The data provide valuable new insights into the role of conserved and variable motifs of BimA in actin-based motility and intercellular spread of B. pseudomallei . at proved optimal glutathione-Sepharose protein-coated beads used directly for actin-binding evaluate the ability of the proteins to polymerize actin, each glutathi- one-Sepharose beads with 10 mM reduced glutathione as directed man-ufacturer using Novagen U-tube concentrator units. pro- tein Bradford reagent analysis of absorbance at 595 nm relative to bovine serum albumin (BSA) standards of known concentrations. expressed pGEX-4T-1 Cell infection studies and ﬂuorescence microscopy. To determine the impact of in-frame deletions or insertions in bimA on actin-based motility, pME6032- based constructs were separately introduced 10276 bimA by electro-poration with selection for tetracycline and chloramphenicol resistance. Strains were grown to stationary phase in Luria-Bertani broth, washed in phosphate-buffered
Telomere Length and Risk of Myocardial Infarction in a Multiethnic Population

repetitive, gene-poor the ends of telomeres due and chronic in ﬂ ammation (1). it suggested telomere length (TL) may be a marker of age-related diseases, as cardiovascular disease (CVD). Previous on the association between shorter TL and the risk of CVD inconsistent results, of conducted with We examined the relationship between TL and myocardial infarction (MI) in a large, multiethnic MI case-control study, the INTERHEART study Data were collected on patient demographic and risk activity, waist-to-hip ratio, and apolipoprotein B/apolipoprotein A-I ratio. Using a validated qualitative polymerase chain reaction method, we measured leukocyte TL in peripheral blood samples from 3,972 subjects and 4,321 control subjects (4). Leukocyte TL is highly correlated with TL in various somatic tissues
Strengths use and deficit correction in organizations

Although the positive psychology tradition emphasizes the importance of a balanced approach regarding individual strengths and weaknesses, there is no valid instrument to measure these phenomena in organizations. The purpose of the present studies is to develop and validate an instrument that measures four dimensions, namely perceived organizational support (POS) for strengths use, POS for de ﬁ cit correction, strengths use behaviour, and de ﬁ cit correction behaviour. In study 1 and 2, the Strengths Use and De ﬁ cit COrrection (SUDCO) questionnaire was developed and tested for its factor structure, reliability, and convergent and criterion validity in two samples of South African employees ( N = 338 and N = 361, respectively). In study 3, the convergent and criterion validity of the SUDCO were examined in a sample of Dutch engineers ( N = 133). Results indicated that the intended dimensions of strengths use and de ﬁ cit correction can be measured reliably with 24 items and showed convergent validity. Moreover, POS for strengths use and strengths use behaviour correlated positively with self- and manager-ratings of job performance, supporting the criterion validity of these scales. As expected, POS for de ﬁ cit correction and de ﬁ cit correction behaviour were unrelated to the performance ratings.
Role of Protein Tyrosine Phosphatase Epsilon (PTP ε ) in LTD 4 -Induced CXCL8 Expression

Phosphorylation on tyrosine residues is recognized as an important mechanism for connecting extracellular stimuli to cellular events and defines a variety of physiological responses downstream of G protein-coupled receptor (GPCR) activation. To date, very few protein tyrosine phosphatases (PTPs) have been shown to associate with GPCRs and little is known about their role in GPCR signaling. In an attempt to discover potential cysteinyl-leukotriene receptor (CysLT 1 R)-interacting proteins, we identified protein tyrosine phosphatase epsilon (PTP ε ) in a yeast two-hybrid assay. Since both proteins are closely linked to asthma, their association was further investigated. Using a HEK-293 cell line stably transfected with the receptor (HEK-LT1) as well as human primary monocytes, we found that PTP ε co-localized with CysLT 1 R in both resting and leukotriene D4 (LTD 4 )-stimulated cells. Co-transfection of HEK-LT1 with PTP ε had no effect on CysLT 1 R expression or LTD 4 -induced internalisation, but inhibited LTD 4 -induced CXC chemokine 8 (CXCL8) promoter transactivation, protein expression and secretion. Moreover, reduced phosphorylation of ERK1/2, but not of p38 or JNK1/2 MAPKs, was observed upon LTD 4 stimulation of HEK-LT1 co-expressing cytosolic (cyt-) PTP ε , but not receptor (R) PTP ε . The increased interaction of cyt-PTP ε and ERK1/2 after LTD 4 stimulation was shown by co-immunoprecipitation. In addition, enhanced ERK1/2 phosphorylation and CXCL8 secretion were found in LTD 4 -stimulated human monocytes transfected with PTP ε -specific siRNAs adding support to a regulatory/inhibitory role of PTP ε in CysLT 1 R signaling. Given that the prevalence of severe asthma is increasing, the identification of PTP ε as a new potential therapeutic target may be of interest. CXCL8 monocytes, time-dependent between ERK1/2 and cyt-PTP ε upon 4 HEK-LT1 cells the substrate-trapping mutant of the phosphatase. the RPTP ε substrate-trapping of LTD 4 ERK1/2 phosphorylation in presence of cyt-PTP expression LTD 4 ERK1/2 phosphorylation CXCL8 expression in primary monocytes, Our results suggest that PTP ε may be important in inflammatory processes modulated by cys-LTs and an understanding of the interaction of CysLT 1 R and PTP ε , both being associated with asthma, may further define their involvement in this disease and potentially provide a novel therapeutic target.
Biochemical and Genetic Analyses of Murine Hepatitis Virus Nsp15 Endoribonuclease (cid:1)

The goal of this project was to better deﬁne the relationship between the endoribonuclease activity of murine hepatitis virus (MHV) Nsp15 (mNsp15) and its role in virus infection. Molecular modeling demonstrated that the catalytic residues of mNsp15 are superimposable with its severe acute respiratory syndrome coronavirus ortholog. Alanine substitutions at three key residues in the mNsp15 catalytic pocket (H262, H277, and G275) and a double-mutant version (H262P and H277A) generated proteins with greatly reduced but detectable endoribonuclease activities. Furthermore, these mutant proteins demonstrated lower cleavage speciﬁcities for uridylate than wild-type (WT) mNsp15. These mutations were successfully incorporated into viruses named vH262A, vH277A, vG275A, and vH262P (cid:1) H277A. All four mutant viruses formed plaques with diameters similar to that of MHV-A59 1000 (WT virus) on several different cell lines. Interestingly, viruses with a mutation at a noncatalytic residue, not be recovered despite repeated attempts, and expression of mNsp15 containing the D324A mutation in Escherichia in an insoluble protein. Plaques derived from vH262A produced approximately 6- to 13-fold fewer PFU than those from WT virus. Cells infected with mNsp15 mutant viruses accumulated lesser amounts of plus- and minus-sense subgenomic RNAs and spike protein than WT virus. The expression of mNsp15 in trans by transient transfection partially restored RNA synthesis by vH262A. These results demonstrate that mNsp15 is required for optimal infection by MHV.
Stark effect in quantum waveguide with Neumann window

In this study, we investigate the bound states of the Hamiltonian describing a quantum particle living on three dimensional straight strip of width $d$ in the presence of an the electric field of constant intensity $F$ in the direction perpendicular to the electron plane. We impose Neumann boundary condition on a disc window of radius $a$ and Dirichlet boundary conditions on the remained part of the boundary of the strip. We prove that such system exhibits discrete eigenvalues below the essential spectrum for any $F,a>0. $
Depressive Symptom Clusters and 5-Year Incidence of Coronary Artery Calcification: The CARDIA Study

: Background - Because depression is a multidimensional construct and few studies have compared the relative importance of its facets in predicting cardiovascular risk, we evaluated the utility of depressive symptom clusters in predicting the 5-year incidence of coronary artery calcification (CAC). Methods and Results - Participants were 2,171 middle-aged adults (58% female, 43% black) from the Coronary Artery Risk Development in Young Adults (CARDIA) Study who were free of cardiovascular disease. Depressive symptom clusters ( z scores) were measured by questionnaires in 2000-2001, and CAC was measured by electron beam computed tomography in 2000-2001 and 2005-2006. There were 243 (11%) cases of incident CAC, defined as the absence of CAC at baseline and the presence of CAC at follow-up. Total depressive symptoms ( OR = 1.16, 95% CI : 1.02-1.33, p = .03) and the depressed affect cluster ( OR = 1.17, 95% CI : 1.03-1.33, p = .02) predicted incident CAC; however, the somatic, interpersonal distress, low positive affect, and pessimism clusters did not. The depressed affect-incident CAC relationship was independent of age, sex, race, education, and antidepressant use; was similar across gender and racial groups; and was partially accounted for by tobacco use and mean arterial pressure. Conclusions - In contrast to recent results indicating that the somatic cluster is the most predictive of cardiovascular outcomes, we found that the prospective association between depressive symptoms and incident CAC was driven by the depressed affect cluster. Our findings raise the possibility that there may not be one facet of depression that is the most cardiotoxic across all contexts. 95% 1.02-1.33, p = . 03) and the depressed affect cluster ( OR = 11.1.17,7,7 C C ininindididicacacatititiveveve ccclililinininicacacallllllyyy sisisigngngnififificicicanananttt sysysympmpmptototomsmsms. WW Womomomenenen hh hadadad hh higigigheheherrr aandndnd wwwasas ssimimimilililaaar r ininin m m maagagnininituutudede aaaccrrossss s gegegendndnderer a aandndd rr racacaciiaiall grrouououpspsps. T T t CACACAC C rreelaaationnnshshhipip fefeelllll shohoortrt ooof aaafttter popopotetenntntiaial
Kobe University Repository : Kernel タイトル Tit le Prion pathogenesis is unaltered in the absence of SIRPα-mediated " don ' teat-me " signaling 著者

Prion diseases are neurodegenerative conditions caused by misfolding of the prion protein, leading to conspicuous neuronal loss and intense microgliosis. Recent experimental evidence point towards a protective role of microglia against prion-induced neurodegeneration, possibly through elimination of prion-containing apoptotic bodies. The molecular mechanisms by which microglia recognize and eliminate apoptotic cells in the context of prion diseases are poorly defined. Here we investigated the possible involvement of signal regulatory protein α (SIRPα), a key modulator of host cell phagocytosis; SIRPα is encoded by the Sirpa gene that is genetically linked to the prion gene Prnp. We found that Sirpa transcripts are highly enriched in microglia cells within the brain. However, Sirpa mRNA levels were essentially unaltered during the course of experimental prion disease despite upregulation of other microglia-enriched transcripts. To study the involvement of SIRPα in prion pathogenesis in vivo, mice expressing a truncated SIRPα protein unable to inhibit phagocytosis were inoculated with rodent-adapted scrapie prions of the 22L strain. Homozygous and heterozygous Sirpa mutants and wild-type mice experienced similar incubation times after inoculation with either of two doses of 22L prions. Moreover, the extent of neuronal loss, microgliosis and abnormal prion protein accumulation was not significantly affected by Sirpa genotypes. Collectively, these data indicate that SIRPα-mediated phagocytosis is not a major determinant in prion disease pathogenesis. It will be important to search for additional candidates mediating prion phagocytosis, as this mechanism may represent an important target of antiprion therapies.
Capital Structure and Performance of Deposit Money Banks in Nigeria

Investigations into the relationship between capital structure and firm performance over the years have consistently produced mixed results in the light of prevailing theories relevant to the concept of capital structure. The study examined the nature of the relationship between the capital structure of Deposit Money Banks (DMBs) in Nigeria and the trend of performance recorded in the industry. Leverage was adopted as a surrogate for capital structure, while firm performance was proxied by profit efficiency and return on equity. A regression analysis test was applied to a balanced panel data, pooled from a sample of 11 DMBs to determine the impact of capital structure on performance. The study found evidence that supports a non-monotonic relationship between capital structure and performance of DMBs, as predicted by the agency cost theoretical model. A major recommendation elicited from the findings of the study advocates for legal control on the proportion of debt DMBs can include in their capital structure if they are to operate as efficiently as expected.
Tumor-Targeted Drug Delivery as a Successful Approach for Reducing Doxorubicin–Induced Cardiotoxicity

Nasibeh Mohammad Ali Pourradi Tabriz University of Medical Sciences Yadollah Azarmi (  azarmiy@tbzmed.ac.ir ) Tabriz University of Medical Sciences Hossien Babaei Tabriz University of Medical Sciences Behzad Baradaran Tabriz University of Medical Sciences Behrooz Shokouhi-Gogani Tabriz University of Medical Sciences Zahra Abbasnejad Tabriz University of Medical Sciences Hamed Hamishehkar Tabriz University of Medical Sciences
Aspectuo-Temporal Underspecification in Anindilyakwa: Descriptive, Theoretical, Typological and Quantitative Issues

Many so-called ‘zero tense’-marked (which we define as morphologically reduced and underspecified inflections) or untensed verb forms found in tenseless languages, have been characterized as context dependent for their temporal and aspectual interpretation, with the verb’s aspectual content (either as event structure or viewpoint properties) being given more or less prominent roles in their temporal anchoring. In this paper, we focus on a morpho-phonologically reduced inflectional verbal paradigm in Anindilyakwa (Groote Eylandt archipelago, NT, Australia), which is both temporally and aspectually underspecified, and constitutes an instance of zero tense as defined above. On the basis of a quantitative study of an annotated corpus of zero-inflected utterances, we establish that in the absence of independent overt or covert temporal information, the temporal anchoring of this ‘zero tense’ exhibits complex patterns of sensitivity to event structural parameters. Notably we establish that while dynamicity/stativity and telicity/atelicity are to some extent valuable predictors for the temporal interpretation of zero tense in Anindilyakwa, only atomicity (i.e., event punctuality) and boundedness categorically impose a past temporal anchoring—this confirms insights found in previous works, both on Anindilyakwa and on other languages, while also differing from other generalisations contained in these works. Our analysis also shows that unlike several zero tenses identified in various languages (especially in Pidgins and Creoles), Anindilyakwa zero tense-marked dynamic utterances do not correlate with a past temporal reading. Rather, we show that Anindilyakwa seems to come closest to languages possessing zero tensed-verbs (or tenseless verbs) where boundedness monotonically enforces a past temporal anchoring, such as Navajo and Mandarin Chinese. We also show that aspect-independent temporal information appears to determine the temporal anchoring of all zero tense-marked unbounded atelic utterances (both stative and dynamic) in Anindilyakwa—a fact at once conflicting with some claims made in previous works on zero tenses, while confirming results from past studies of Indigenous languages of the Americas (especially Yucatec Maya), concerning the role of temporal anaphora in the temporal interpretation of ‘tenseless’ verb forms.
EHRA/HRS/APHRS/SOLAECE expert consensus on Atrial cardiomyopathies: De ﬁ nition, characterisation, and clinical implication $ , $$ Journal of Arrhythmia

EHRA/HRS/APHRS/SOLAECE expert consensus on Atrial cardiomyopathies: Definition, characterisation, and clinical implication Andreas Goette, (EHRA chair), Jonathan M. Kalman, (APHRS co-chair), Luis Aguinaga, (SOLAECE co-chair), Joseph Akar , Jose Angel Cabrera , Shih Ann Chen , Sumeet S. Chugh , Domenico Corradi , Andre D'Avila , Dobromir Dobrev , Guilherme Fenelon , Mario Gonzalez , Stephane N. Hatem, Robert Helm, Gerhard Hindricks , Siew Yen Ho , Brian Hoit , Jose Jalife , Young-Hoon Kim , Gregory Y.H. Lip , Chang-Sheng Ma , Gregory M. Marcus , Katherine Murray, Akihiko Nogami , Prashanthan Sanders , William Uribe , David R. Van Wagoner , Stanley Nattel, (HRS co-chair)
Quantum Teleportation and Entanglement: A Hybrid Approach to Optical Quantum Information Processing

Part I: Introductions and Basics 1. Introduction to Quantum Information Processing 2. Introduction to Optical Quantum Information Processing Part II: Fundamental Resources and Protocols 3. Entanglement 4. Quantum Teleportation 5. Quantum Error Correction Part III: Measurement-based and Hybrid Approaches 6. Quantum Teleportation of Gates 7. Cluster-based Quantum Information Processing 8. Hybrid Quantum Information Processing
Antenatal care utilization in recently delivered rural females: A hospital‑based cross‑sectional study

Background: Adequate antenatal care (ANC) services hold the key for the health of both the pregnant mother and her newborn. Utilization of ANC services is of paramount importance for reducing maternal mortality in India where maternal mortality rate remains a cause of concern despite several measures undertaken in this regard. Objectives: The study aimed to assess the utilization of ANC services in the recently delivered rural females. Materials and Methods: The current study was a hospital-based cross-sectional study. A predesigned and pretested questionnaire was administered to the rural females who had delivered in tertiary care teaching hospital in Jammu, Jammu and Kashmir, India. The data were tabulated, analyzed, and presented in proportions. Chi-square was the test of significance with P < 0.05 considered statistically significant. Results: During the study, 310 rural recently delivered females were interviewed. One-third of them were receiving ANC from subhealth centre and 58.70% of the total had registered before 12 weeks of gestation. Only one-third had taken >100 iron-folic acid tablets during pregnancy. Among various variables, age, religion, literacy, type of family, and occupation of mother were found to be statistically significant in relation to ANC visits (P < 0.05). Conclusion: Despite best efforts of Government of India in context to maternal and child health services in the rural areas of the country, the results show that there remain a few areas of concern. The need is to upgrade the quality of services as well as public health infrastructure, especially in the vast hinterlands of the nation.
CMAR_A_348890 287..301

Purpose: This study aimed to study the prognostic value of clinicopathological data, inflammation and nutritional indicators, and to design an effective prognostic nomogram and heat map to predict cancer-specific survival (CSS) and disease-free survival (DFS) of stage II/III GC patients who underwent curative gastrectomy with adjuvant chemotherapy. Patients and Methods:We retrospectively analyzed the data of 611 patients with stage II/III GC after curative gastrectomy followed by adjuvant chemotherapy from 3 GC disease centers. Patients were divided into a training cohort (n = 503) and an external validation cohort (n = 108). Nomograms were established based on independent predictors identified by Cox regression analysis in the training cohort. The consistency index (C-index) and the calibration curve were used to evaluate the discriminative ability and accuracy of the nomogram. Heat maps were constructed with the prognostic factors and the corresponding survival probability. We further divided the patients into low-risk and high-risk groups based on the risk score of the nomogram. Results: Through univariate and multivariate survival analysis, the independent risk factors common to CSS and DFS were identified. Then these predictors were incorporated into the nomograms, and the established nomograms used to predict CSS and DFS had high discriminative power in the training cohort. Meanwhile, the calibration curves of CSS and DFS probability also showed good agreement between the prediction based on the nomograms and the actual observation results. The above independent predictors were applied to establish heat maps. Compared with low-risk patients, the high-risk patients calculated according to the nomogram had a shorter survival time and a worse prognosis. Conclusion: We established a nomogram and heat map, which could be used to assess the survival rate of stage II/III GC patients who underwent curative gastrectomy with adjuvant chemotherapy. These tools had high prognostic prediction accuracy and provided inspiration for clinical decision-making.