Miracle-dz/UniFAR-FTUs huggingface dataset:
The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance

This study adds insights into FTSJ1 tRNA 2′-O-methylation–associated pathologies in humans and Drosophila by identification of novel FTSJ1 targets and neuron morphology defects. FTSJ1 is a conserved human 2′-O-methyltransferase (Nm-MTase) that modifies several tRNAs at position 32 and the wobble position 34 in the anticodon loop. Its loss of function has been linked to X-linked intellectual disability (XLID), and more recently to cancers. However, the molecular mechanisms underlying these pathologies are currently unclear. Here, we report a novel FTSJ1 pathogenic variant from an X-linked intellectual disability patient. Using blood cells derived from this patient and other affected individuals carrying FTSJ1 mutations, we performed an unbiased and comprehensive RiboMethSeq analysis to map the ribose methylation on all human tRNAs and identify novel targets. In addition, we performed a transcriptome analysis in these cells and found that several genes previously associated with intellectual disability and cancers were deregulated. We also found changes in the miRNA population that suggest potential cross-regulation of some miRNAs with these key mRNA targets. Finally, we show that differentiation of FTSJ1-depleted human neural progenitor cells into neurons displays long and thin spine neurites compared with control cells. These defects are also observed in Drosophila and are associated with long-term memory deficits. Altogether, our study adds insight into FTSJ1 pathologies in humans and flies by the identification of novel FTSJ1 targets and the defect in neuron morphology.
Measuring the Influence of Noise Reduction on Listening Effort in Hearing-Impaired Listeners Using Response Times to an Arithmetic Task in Noise

Single microphone noise reduction (NR) in hearing aids can provide a subjective benefit even when there is no objective improvement in speech intelligibility. A possible explanation lies in a reduction of listening effort. Previously, we showed that response times (a proxy for listening effort) to an auditory-only dual-task were reduced by NR in normal-hearing (NH) listeners. In this study, we investigate if the results from NH listeners extend to the hearing-impaired (HI), the target group for hearing aids. In addition, we assess the relevance of the outcome measure for studying and understanding listening effort. Twelve HI subjects were asked to sum two digits of a digit triplet in noise. We measured response times to this task, as well as subjective listening effort and speech intelligibility. Stimuli were presented at three signal-to-noise ratios (SNR; –5, 0, +5 dB) and in quiet. Stimuli were processed with ideal or nonideal NR, or unprocessed. The effect of NR on response times in HI listeners was significant only in conditions where speech intelligibility was also affected (–5 dB SNR). This is in contrast to the previous results with NH listeners. There was a significant effect of SNR on response times for HI listeners. The response time measure was reasonably correlated (R142 = 0.54) to subjective listening effort and showed a sufficient test–retest reliability. This study thus presents an objective, valid, and reliable measure for evaluating an aspect of listening effort of HI listeners.
Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs

Summary In Europe, swine represent economically important farm animals and furthermore have become a preferred preclinical large animal model for biomedical studies, transplantation and regenerative medicine research. The need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ‐transplantation experiments and their use in infection studies and for design of veterinary vaccines. In this study, we characterised the SLA class I (SLA‐1, SLA‐2, SLA‐3) and class II (DRB1, DQB1, DQA) genes of 549 farmed pigs representing nine commercial pig lines by low‐resolution (Lr) SLA haplotyping. In total, 50 class I and 37 class II haplotypes were identified in the studied cohort. The most common SLA class I haplotypes Lr‐04.0 (SLA‐1*04XX‐SLA‐3*04XX(04:04)‐SLA‐2*04XX) and Lr‐32.0 (SLA‐1*07XX‐SLA‐3*04XX(04:04)‐SLA‐2*02XX) occurred at frequencies of 11.02 and 8.20% respectively. For SLA class II, the most prevalent haplotypes Lr‐0.15b (DRB1*04XX(04:05/04:06)‐DQB1*02XX(02:02)‐DQA*02XX) and Lr‐0.12 (DRB1*06XX‐DQB1*07XX‐DQA*01XX) occurred at frequencies of 14.37 and 12.46% respectively. Meanwhile, our laboratory has contributed to several vaccine correlation studies (e.g. Porcine Reproductive and Respiratory Syndrome Virus, Classical Swine Fever Virus, Foot‐and‐Mouth Disease Virus and Swine Influenza A Virus) elucidating the immunodominance in the T‐cell response with antigen specificity dependent on certain SLA‐I and SLA‐II haplotypes. Moreover, these SLA–immune response correlations could facilitate tailored vaccine development, as SLA‐I Lr‐04.0 and Lr‐32.0 as well as SLA‐II Lr‐0.15b and Lr‐0.12 are highly abundant haplotypes in European farmed pigs.
Dental Pain in Homeless Adults in Porto Alegre, Brazil

Objective To evaluate the association of dental pain with time living on the street in a sample of homeless people in the city of Porto Alegre, Brazil. Methods A cross-sectional study was carried out with homeless people who accessed social services in 2017. A questionnaire was administered covering socioeconomic variables and including questions about general health, use of tobacco/alcohol/drugs, use of dental services and history of dental pain. The decayed, missing, and filled teeth (DMFT) index was calculated in an oral examination. The association between socioeconomic characteristics, health status, time of homelessness and dental pain was evaluated using chi-square, t-, and Mann-Whitney tests. Results A total sample of 214 homeless people was surveyed. Most were male (76.2%), had a low level of education (67.6%) and experienced many episodes of dental pain during life (91.0%). The last episode of pain was moderate or intense (79.7%) and do-it-yourself measures for pain relief were preferred (62.3%). Individuals who were homeless for longer than 1 year had more dental pain in the past (P < 0.001), more frequency of a recent episode of dental pain (P = 0.03), and sought a dentist or healthcare service to relieve pain less frequently (P = 0.03). Conclusions Dental pain is frequent in the context of homelessness and does not necessarily result in seeking dental services, even where there is universal oral health care.
Can filled pauses be represented as linguistic items? Investigating the effect of exposure on the perception and production of um

The current paper presents three studies that investigated the effect of exposure on the mental representations of filled pauses (um/uh). In Study 1, a corpus analysis identified the frequency of co-occurrence of filled pauses with words located immediately before or after them in naturalistic spoken adult British English (BNC2014). Based on the collocations identified in Study 1, in Study 2, 22 native British English-speaking adults heard sentences in which the location of filled pauses and the co-occurring words were manipulated and the participants were asked to judge the acceptability of the sentences heard. Study 3 was a sentence recall experiment in which we asked 29 native British English adults to repeat a similar set of sentences as used in Study 2. We found that frequency-based distributional patterns of filled pauses (Study 1) affected the sentence judgments (Study 2) and repetition accuracy (Study 3), in particular when the filled pause followed its collocate. Thus, the current study provides converging evidence for the account maintaining that filled pauses are linguistic items. In addition, we suggest filled pauses in certain locations could be considered as grammatical items, such as suffixes.
Effect of tranexamic acid in arthroscopic anterior cruciate ligament repair: A systematic review and meta-analysis of randomised clinical trials

Purpose: Perioperative blood loss remains a major challenge to surgeons in anterior cruciate ligament reconstruction (ACLR) surgery, despite of the introduction of minimally invasive approach. Tranexamic acid (TXA) is believed to reduce blood loss, which may minimise the complication of postoperative haemarthrosis with insufficient evidence on its effectiveness in ACLR. The primary aim of this study was to examine the effect of TXA on postoperative blood loss and other secondary outcomes in patients undergoing arthroscopic ACLR surgery. Method: PUBMED, EMBASE, MEDLINE and CENTRAL database were systematically searched from its inception until November 2020. All randomised clinical trials (RCTs) comparing TXA (intravenous or intra-articular) versus placebo in the arthroscopic ACLR surgery were included. Case series, case report and editorials were excluded. Results: Five RCTs comprising of a total of 580 patients (291 in TXA group, 289 in control group) were included for qualitative and quantitative meta-analysis. In comparison to placebo, TXA group was significantly associated with lower postoperative blood loss (mean difference (MD): −81.93 ml; 95% CI −141.80 to −22.05) and lower incidence of needing knee aspiration (odd ratio (OR): 0.19; 95% CI 0.08 to 0.44). Patients who randomised to TXA were also reported to have better range of movement (MD: 2.86; 95% CI 0.54 to 5.18), lower VAS Pain Score (MD: −1.39; 95% CI −2.54 to −0.25) and higher Lysholm Score (MD: 7.38; 95% CI 2.75 to 12.01). Conclusion: In this meta-analysis, TXA reduced postoperative blood loss with lesser incidence of needing knee aspiration along with better range of knee movement and Lysholm score in patients undergoing arthroscopic ACLR surgery.
Is quetiapine effective for obsessive and compulsive symptoms in patients with bipolar disorder? A randomized, double-blind, placebo-controlled clinical trial

Abstract Background The aim of this study is to examine the effects of quetiapine as an adjuvant treatment for obsessive–compulsive (OC) symptoms in patients with bipolar disorder (BD) type I. Methods In this 8-week double-blind placebo-controlled randomized clinical trial, 47 patients with BD in euthymic phase that had OC symptoms were randomly allocated to receive either quetiapine or placebo plus their routine medications (lithium + clonazepam). Yale–Brown Obsessive–Compulsive Scale (YBOCS) was used to assess the outcomes. Adverse effects were also recorded. Results Of 47 BD patients with OC symptoms that were randomly allocated in two groups of quetiapine (n = 24) and placebo group (n = 23), 40 patients (20 in quetiapine group and 20 in placebo group) completed the trial. Throughout the trial, the mean score of YBOCS in the quetiapine group dropped from 24.37 ± 1.51 to 15.26 ± 1.16 (P < .001) and in the placebo group decreased from 24.21 ± 1.33 to 23.94 ± 1.66 (P = 1.97). At the end of the study, 12 (60%) patients in the quetiapine group and 1 (5%) patient in the placebo group had more than 34% decline in YBOCS score (P < .001). No serious adverse effects were reported in two groups. Conclusions Our double-blind placebo-controlled clinical trial showed that quetiapine may be an effective adjuvant agent for reducing OC symptoms in BD patients.
Recurrence of primary sclerosing cholangitis after liver transplantation – analysing the European Liver Transplant Registry and beyond

Liver transplantation for primary sclerosing cholangitis (PSC) can be complicated by recurrence of PSC (rPSC). This may compromise graft survival but the effect on patient survival is less clear. We investigated the effect of post‐transplant rPSC on graft and patient survival in a large European cohort. Registry data from the European Liver Transplant Registry regarding all first transplants for PSC between 1980 and 2015 were supplemented with detailed data on rPSC from 48 out of 138 contributing transplant centres, involving 1,549 patients. Bayesian proportional hazards models were used to investigate the impact of rPSC and other covariates on patient and graft survival. Recurrence of PSC was diagnosed in 259 patients (16.7%) after a median follow‐up of 5.0 years (quantile 2.5%‐97.5%: 0.4–18.5), with a significant negative impact on both graft (HR 6.7; 95% CI 4.9–9.1) and patient survival (HR 2.3; 95% CI 1.5–3.3). Patients with rPSC underwent significantly more re‐transplants than those without rPSC (OR 3.6, 95% CI 2.7–4.8). PSC recurrence has a negative impact on both graft and patient survival, independent of transplant‐related covariates. Recurrence of PSC leads to higher number of re‐transplantations and a 33% decrease in 10‐year graft survival.
GIS-based crash hotspot identification: a comparison among mapping clusters and spatial analysis techniques

Abstract Knowing the locations of traffic crash hotspots can provide us with valuable insights into the root causes of crash occurrence over the area under study. This knowledge helps decision-makers to better assess the risk associated with road crashes and, as a result, help them to propose more effective countermeasures in order to reduce the annual crash rate. Nonetheless, identifying the areas with the highest potential of crash occurrence is a complicated task. In this regard and within this study, five various types of hotspot identification techniques, consisting of Average Nearest Neighbor, Getis-Ord Gi*, Global Moran’s I, kernel density estimation (KDE) and mean centre, were compared to each other, using three different performance measures, including Predictive Accuracy Index (PAI), Recapture Rate Index (RRI) and hit rate. According to the results, the most accurate model with the highest PAI values (PAI = 1.61 and 1.76), Moran’s I, had the third-highest reliability value (RRI = 1.003). On the other hand, while the Gi* method was the most precise and reliable technique with the highest RRI value (RRI = 1.121), it showed the second-lowest accuracy (PAI= 0.83 and 0.74). Overall, it seems that Moran’s I method is superior to other methods in locating hotspots, which is not only the most accurate technique but also precise enough to rely on.
Benzotriazole concentrations in airport runoff are reduced following changes in airport deicer formulations

A comparison of the presence of additives in airport deicers commonly used in the United States and in airport runoff was conducted with data collected before and after changes in deicer formulations. Three isomers of benzotriazoles (BTs)—4‐methyl‐1H‐benzotriazole (4‐MeBT), 5‐methyl‐1H‐benzotriazole (5‐MeBT), and 1H‐benzotriazole (1H‐BT)—are corrosion inhibitors added to some formulations of airport deicers and are reported to be a source of aquatic toxicity in streams receiving airport runoff. Concentrations of BT in aircraft deicers and anti‐icing fluids (ADAF) were reduced over time but were not reduced in potassium acetate airfield‐pavement deicer material (PDM) that was used throughout the study period. Streams receiving runoff from Milwaukee Mitchell International Airport, Milwaukee, Wisconsin, USA, were monitored from 2004 to 2019 for BTs, with concentrations of 4‐MeBT varying from <0.35 to 4600 µg/L, 5‐MeBT varying from <0.25 to 6600 µg/L, and 1H‐BT varying from <0.25 to 150 µg/L. Median 4‐MeBT concentrations at sites downstream from the airport decreased by approximately 74%, 5‐MeBT by 69%, and 1H‐BT by 82% following reduction in BTs in ADAF formulations, resulting in a reduction in the potential for aquatic toxicity in receiving streams. A change in residuals from regression analysis between freezing point depressants and BTs indicate that the reduction in BT concentrations in airport runoff was a result of BT reduction in ADAF formulations, but PDM may still be a substantial source of BTs in airport runoff. Because BTs are a source of aquatic toxicity in airport deicers, the reductions in BTs in the common deicers observed in this study can be used to demonstrate the potential for a reduction in the effects to aquatic organisms in airport runoff, resulting in greater likelihood of meeting aquatic toxicity requirements in airport stormwater permits, and potentially driving airports, airlines, and permit holders to advocate further reduction or elimination of BTs and other harmful contaminants in airport deicers. Integr Environ Assess Manag 2022;18:245–257. Published 2021. This article is a U.S. Government work and is in the public domain in the USA. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC)
Comparison of pregnancy outcomes using a time-lapse monitoring system for embryo incubation versus a conventional incubator in in vitro fertilization: An age-stratification analysis

Objective The aim of this study was to compare the pregnancy outcomes of in vitro fertilization with embryo transfer between embryos cultured in a time-lapse monitoring system (TLS) and those cultured in a conventional incubator (CI). Methods The medical records of 250 fertilized embryos from 141 patients undergoing infertility treatment with assisted reproductive technology at a tertiary hospital from June 2018 to May 2020 were reviewed. The study population was divided into TLS and CI groups at a 1 to 1 ratio (125 embryos per group). The primary outcome was the live birth rate. Results The TLS group had a significantly higher clinical pregnancy rate (46.4% vs. 27.2%, p=0.002), implantation rate (27.1% vs. 12.0%, p=0.004), and live birth rate (32.0% vs. 18.4%, p=0.013) than the CI group. Furthermore, subgroup analyses of the clinical pregnancy rate and live birth rate in the different age groups favored the TLS group. However, this difference only reached statistical significance in the live birth rate in women aged over 40 years and the clinical pregnancy rate in women aged 35–40 years (p=0.048 and p=0.031, respectively). The miscarriage rate, cleavage rate, and blastocyst rate were comparable. Conclusion TLS application improved the live birth rate, implantation rate, and clinical pregnancy rate, particularly in the advanced age group in this study, while the other reproductive outcomes were comparable. Large randomized controlled trials are needed to further explore the ramifications of these findings, especially in different age groups.
Is diabetic neuropathy associated with vitamin D status? A meta-analysis

Abstract Several studies have been conducted to investigate the relation between 25-hydroxyvitamin D [25(OH)D] level and diabetic neuropathy (DN). However, there is still no clear conclusion due to differences in study design and cut-off values used in the published work, in addition to the absence of a comprehensive meta-analysis (MA) on the topic. The present systematic review and MA therefore aims at clarifying the association between vitamin D level and peripheral DN in patients with type 2 diabetes mellitus. Primary research studies that explored the association between 25(OH)D level and diabetic peripheral neuropathy in type 2 diabetes were located from Medline, EMBASE, Web of Science, Cochrane Library, CINHAL and Google Scholar. Twenty-six studies met the inclusion criteria with 6277 participants where 2218 were diabetic with DN, 2959 were diabetic without DN and 406 were healthy. Diabetic patients with DN showed significantly lower serum 25(OH)D compared with patients without DN (standardised mean difference (SMD) of −0·92 (95 % CI −1·18, −0·65, I 2 = 93·3 %, P < 0·0001). The pooled OR value of vitamin D deficiency was higher in patients with DN, 1·84 (95 % CI 1·46, 2·33, P < 0·0001) and 2·87 (95 % CI 1·10, 7·52, P = 0·03) when using fixed-effects and random-effects models, respectively. Vitamin D deficiency has been found to be highly prevalent among diabetic patients with neuropathy. Since 25(OH)D has been implicated in glucose haemostasis and showed benefit in reducing neuropathy symptoms, its supplementation is warranted for this population of patients.
Swedish Normative Data for Mindmore: A Comprehensive Cognitive Screening Battery, Both Digital and Self-Administrated

Abstract Objective: Cognitive impairment is a key element in most mental disorders. Its objective assessment at initial patient contact in primary care can lead to better adjusted and timely care with personalised treatment and recovery. To enable this, we designed the Mindmore self-administrative cognitive screening battery. What is presented here is normative data for the Mindmore battery for the Swedish population. Method: A total of 720 healthy adults (17 to 93 years) completed the Mindmore screening battery, which consists of 14 individual tests across five cognitive domains: attention and processing speed, memory, language, visuospatial functions and executive functions. Regression-based normative data were established for 42 test result measures, investigating linear, non-linear and interaction effects between age, education and sex. Results: The test results were most affected by age and to a lesser extent by education and sex. All but one test displayed either linear or accelerated age-related decline, or a U-shaped association with age. All but two tests showed beneficial effects of education, either linear or subsiding after 12 years of educational attainment. Sex affected tests in the memory and executive domains. In three tests, an interaction between age and education revealed an increased benefit of education later in life. Conclusion: This study provides normative models for 14 traditional cognitive tests adapted for self-administration through a digital platform. The models will enable more accurate interpretation of test results, hopefully leading to improved clinical decision making and better care for patients with cognitive impairment.
Level of Effort: A Reliable and Practical Alternative to the Velocity-Based Approach for Monitoring Resistance Training

Abstract Hernández-Belmonte, A, Courel-Ibáñez, J, Conesa-Ros, E, Martínez-Cava, A, and Pallarés, JG. Level of effort: A reliable and practical alternative to the velocity-based approach for monitoring resistance training. J Strength Cond Res 36(11): 2992–2999, 2022—This study analyzed the potential of the level of effort methodology as an accurate indicator of the programmed relative load (percentage of one-repetition maximum [%1RM]) and intraset volume of the set during resistance training in the bench press, full squat, shoulder press, and prone bench pull exercises, through 3 specific objectives: (a) to examine the intersubject and intrasubject variability in the number of repetitions to failure (nRM) against the actual %1RM lifted (adjusted by the individual velocity), (b) to investigate the relationship between the number of repetitions completed and velocity loss reached, and (c) to study the influence of the subject's strength level on the aforementioned parameters. After determining their individual load-velocity relationships, 30 subjects with low (n = 10), medium (n = 10), and high (n = 10) relative strength levels completed 2 rounds of nRM tests against their 65, 75, 85, and 95% 1RM in the 4 exercises. The velocity of all repetitions was monitored using a linear transducer. Intersubject and intrasubject variability analyses included the 95% confidence intervals (CIs) and the the standard error of measurement (SEM), respectively. Coefficient of determination (R2) was used as the indicator of relationship. nRM showed a limited intersubject (CI ≤ 4 repetitions) and a very low intrasubject (SEM ≤1.9 repetitions) variability for all the strength levels, %1RM, and exercises analyzed. A very close relationship (R2 ≥ 0.97) between the number of repetitions completed and the percentage of velocity loss reached (from 10 to 60%) was found. These findings strengthen the level of effort as a reliable, precise, and practical strategy for programming resistance training.