Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcription Factors Expression of Differentiation-Associated B-1 Cells Violate the Accepted Paradigm for Cutting Edge: Spontaneously Ig-Secreting

B-1 cells spontaneously secrete natural Ig that acts as a primary line of defense against infection. A major short-fall in our understanding of this key process centers on the molecular mechanisms regulating natural Ab secretion by B-1 cells. Herein, we demonstrate that secreting B-1 cells use some aspects of the recently recognized plasmacytic differentiation program but deviate from it in important ways. Specifically, we show that key repressors of the plasmacytic program, B cell leukemia/lymphoma-6 and paired box gene 5, are reduced in spontaneously secreting B-1 B cells, as in stimulated differentiated B-2 cells. Sur-prisingly, we find that key promoters of the plasmacytic program, B lymphocyte inducer of maturation program 1 and X-box binding protein 1, are not up-regulated in secreting B-1 cells, in contrast to secreting B-2 cells. These data demonstrate that B-1 cells operate under a differentiation program that is unique and differs from the paradigm associated with Ig-secreting B-2 cells. The Journal of Immunology, 2005, 174: 3173–3177.
An Integrated Mass Spectrometry-based Proteomic Approach QUANTITATIVE ANALYSIS OF TANDEM AFFINITY-PURIFIED IN VIVO CROSS-LINKED PROTEIN COMPLEXES (QTAX) TO DECIPHER THE 26 S PROTEASOME-INTERACTING NETWORK*

We developed an integrated proteomic approach to decipher in vivo protein-protein interactions and applied this strategy to globally map the 26 S proteasome interaction network in yeast. We termed this approach QTAX for quantitative analysis of tandem affinity purified in vivo cross-linked (X) protein complexes. For this work, in vivo formaldehyde cross-linking was used to freeze both stable and transient interactions occurring in intact cells prior to lysis. To isolate cross-linked protein complexes with high purification efficiency under fully denaturing conditions, a new tandem affinity tag consisting of a hexahistidine sequence and an in vivo biotinylation signal was adopted for affinity-based purification. Tandem affinity purification after in vivo cross-linking was combined with tandem mass spectrometry coupled with a quantitative SILAC (stable isotope labeling of amino acids in cell culture) strategy to carry out unambiguous protein identification and quantification of specific protein interactions. Using this method, we captured and identified the full composition of yeast 26 S proteasome complex as well as the two known ubiquitin receptors, Rad23 and Dsk2. Quantitative mass spectrometry analysis allowed us to distinguish specific proteasome-interacting proteins (PIPs) from background proteins and led to the identification of a total of 64 potential PIPs of which 42 are novel interactions. Among the 64 putative specific PIPs, there are ubiquitin pathway components, ubiquitinated substrates, chaperones, and transcription and translation regulators, demonstrating the efficacy of the developed approach in capturing in vivo protein interactions. The method offers an advanced technical approach to elucidate the dynamic protein interaction networks of the proteasome and can find a wide range of applications in the studies of other macromolecular
Antibiogram and plasmid profile of Escherichia coli Isolates from Faeces of Cattle in Abeokuta, South West NIGERIA

Faecal samples collected from cattle in Abeokuta, south west Nigeria were analysed for E. coli counts, antibiotics susceptibility pattern of E. coli and the plasmid profile of E. coli isolates using standard techniques. E. coli were recovered from 30samples representing 28.4% of 107 feacal samples of cattle collected. The faecal E. coli count ranges from 1.1 x 10 3 to 9.0 x 10 3 cfu/ml -1 . The percentage prevalence of resistance to each antimicrobial agent tested were; Ampicillin, AMP (97%), COT (93%), Gentamicin, GEN (57%), Nalidicic acid, NAL (40%), Nitrofuratoin, NIT (93%), Cotrimoxazole, COL (20%), Streptomycin, STR (37%) and Tetracycline, TET (93%) The plasmid profile analysis of the E. coli isolates from cattle faeces shows a total of 18 different plasmid bands with molecular weight ranging from 2310 to 23632.83bp. The relatively high prevalence of resistance to antimicrobial agents exhibited by E. coli isolates from cattle faeces is an indication of the possible health risk that may occur as a result of the indiscriminate release of such faeces to the
Inﬂuence of ESGC Indicators on Financial Performance of Listed Pharmaceutical Companies

: The pharmaceutical industry, concerned about the impact of its activity, has integrated responsible principles and practices with a view to improving its sustainable and ﬁnancial performance. This study analyzes the relationship between environmental, social, governance, and controversy indicators and ﬁnancial performance, measured through return on equity (ROA), return on assets (ROE), and Tobin’s Q, which are applied to the listed companies in the Nasdaq US Smart Pharmaceuticals Index. This index is composed of 30 international companies with a presence at the global level. All the data have been extracted from the Thomson Reuters database. The analysis was performed using structural equation modeling implemented with partial least squares. The results conﬁrm the positive relationship between the construct composed of environmental, social, and governance (ESG) indicators and the aforementioned ﬁnancial ratios. Additionally, a positive relationship of the controversy indicator with Tobin’s Q is supported. This suggests that the pharmaceutical multinationals focus their investments in sustainability on ESG and pay attention to controversies to boost the visibility of the company and thus increase its value. These conclusions conﬁrm that investing in ESG is a proﬁtable strategy. It is also relevant for managers as it increases the proﬁts and the market value of multinational pharmaceutical companies.
Enabling Trajectory Constraints for Usage Control Policies With Backtracking Particle Filters

—A number of studies extended access control policies with constraints, aiming at the restriction of mobile users’ access to appropriate authorized areas. Recent research proposed to rely on usage control instead, in order to allow for continuous checks of the user’s location. A drawback of those approaches is that they rely on crisp trajectory estimates, i.e., spatio-temporal paths, not considering occurring uncertainty. This makes those approaches impractical for indoor applications, where occurring measurement errors are typically large compared to authorized areas. Thus, in this study, we propose extensions for usage control policies to constrain users to an authorized area for the duration of access. We adhere probabilistic trajectories derived from backtracking particle ﬁlters combined with WiFi ﬁngerprinting. However, the main contribution is a risk-based model for deriving usage decisions based on risk factors instead of conventional thresholds. Our results show, that particle ﬁlters are crucial due to inaccuracy in WiFi positioning. We achieve a true-and false-positive rate of 80% and 6 . 7% . Finally, this allows to effectively constrain access to appropriate areas in indoor scenarios.
Speci�c Urban Elements Identi�ed for Tuberculosis Epidemic in Guangzhou by Using Geographical Detector

Background: The prevalence of tuberculosis (TB) in China has heavily affected people’s health for decades, which has been widely investigated for the rural regions and west parts. However, its spatial features in urban areas remain little understood. Thus, this study aims to identify its spatial differentiations and their in�uencing factors in highly urbanized region on a �ne scale. Methods: Together with the TB cases in 2017 obtained from Guangzhou Institute of Tuberculosis Control and Prevention, in total 18 socioeconomic and environmental variables were included in this study. Two spatial analysis tools were respectively applied to select the relative appropriate spatial scale (global Moran’s I), and to identify speci�c urban factors (the Geographical detector) for this epidemic in the central four districts of Guangzhou. Results: The 2 km × 2 km grid was determined as the most appropriate spatial scale due to its relatively higher spatial autocorrelation (Moran’s I=0.33, Z=4.71). At this spatial level, the TB epidemic in the four central districts was obviously closely associated with most of socioeconomic factors (0.31<r<0.76) at the signi�cance level of 0.01. By contrast, among environmental factors, only the concentration of �ne particulate matter (PM 2.5 ) correlated with this epidemic (r=0.21) at the signi�cance level of 0.05. Similarly, according to the q-values derived from geographical detector analysis, socioeconomic factors posed stronger impacts (0.08<q<0.57) on the spatial differentiations of TB prevalence than those of environmental variables (0.06<q<0.27), Furthermore, 153 pairs of variables presented more powerful explanatory abilities for this epidemic’s spatial disparities due to their notable enhancements of q-values (7.3%<sq<311.6%) caused by the pairwise interactions. Conclusion: The spatial heterogeneity of TB prevalence was remarkably in�uenced by a series of speci�c urban elements and their pairwise interactions across the central region of Guangzhou. We accordingly suggest that more attentions should be paid to the areas with pairwise interactions of these speci�c urban elements in this city. This study would provide meaningful clues for local authorities making more targeted interventions on this disease in China’s municipal areas featured by
A New Refutation Calculus With Logical Optimizations for PLTL

—Propositional Linear Temporal Logic (PLTL) is a tool for reasoning about systems whose states change in time. We present an ongoing work on a new proof-search procedure for Propositional Linear Temporal Logic and its implementation. The proof-search procedure is based on a one-pass tableau calculus with a multiple-conclusion rule treating temporal-operators and on some logical optimization rules. These rules have been devised by applying techniques developed by the authors for logics with Kripke semantics and here applied to the Kripke-based semantics for Propositional Linear Temporal Logic.
Importance of fatiguing, overtraining and chronic fatigue in athletes

Each training that requires achieving a higher heart rate limit and/or increased concentration in time may cause fatigue, considered to be a natural defence mechanism of a body. In the case of excessive fatigue and insufficient time designated for rest and regeneration, an overtraining syndrome ( OTS ) may develop. The main symptom of overtraining is increased fatigue that fails to become reversed in normal conditions of regeneration. Although authors are familiar with the overtraining syndrome and associated symptoms, as of today no diagnostic tool has been developed that may form a basis for a final diagnosis, and the diagnosis itself is frequently based on a subjective assessment of the athlete. Possible causes of the band overtraining syndrome are disorders of sodium, inflammatory processes resulting from physical activity and / or disorders of the autonomic nervous system.
New insights from multi-proxy data from West Antarctic drifts : 1 Implications for dating and interpreting Late Quaternary 2 palaeoenvironmental records from the Antarctic margin 3 4

a British Antarctic Survey, High Cross, Madingley Road, Cambridge CB3 0ET, UK 10 b Department of Earth Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EQ, UK 11 c Insitute for Geophysics and Geology, University of Leipzig, Talstrasse 35, Leipzig 04103, Germany 12 d School of Ocean and Earth Science, National Oceanography Centre Southampton, University of 13 Southampton, Waterfront Campus, European Way, Southampton SO14 3ZH, UK 14 e Department of Earth Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK 15 f College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4RJ, UK 16 g College of Marine Science, University of South Florida, St Petersburg, Florida, USA 17 h Alfred-Wegener-Institut Helmholtz-Zentrum für Polarund Meeresforschung, Bremerhaven, 18 Germany 19 I Department of Geological Sciences, University of Florida, Gainesville, Florida, USA 20 j Department of Geography, Durham University, Durham DH1 3LE, UK 21 22
Demographic and Occupational Risk Factors of Carpal Tunnel Syndrome among Dental students in their Final Year at King Abdulaziz University, Jeddah, Saudi Arabia

Carpal tunnel syndrome (CTS) is a common peripheral compression neuropathy that accounts for 90% of all entrapment neuropathies. Dentists typically have a higher prevalence of work-related musculoskeletal disorders including CTS. Currently, there is a lack of literature on CTS prevalence and risk factors among dentistry in Saudi Arabia. Our study was the first to examine the prevalence and the associated demographic and occupational factors of CTS among dental students. This cross-sectional study was conducted in the School of Dentistry at King Abdulaziz University. A total of 120 dental students in their final year were included. We used a validated self-administered questionnaire that included demographic data, work, medical history, and a modified Katz's hand diagram to assess the symptoms and occupational exposures. Descriptive statistics and logistic regression were applied using SPSS software version 16. The prevalence of CTS among dental students was 13.3%. It was higher in females 10% compared to male 3.3%. There was a significant relationship between the body mass index (P= 0.03) with underweight category by having a higher prevalence of CTS. The use of finger pinch grip showed an inverse association with CTS (P=0.04). Other risk factors were not significantly associated with CTS. The prevalence of CTS among dental students was higher than the general population. However, many previously identified risk factors showed no significant association with CTS adding to the controversy of the contributing risk factors of this disease among dentistry. Finger pinch grip showed inverse association which was contrary to published literature. Future studies may include exposure time for each occupational risk factor preferably in a prospective cohort.
Immunolocalization of the electrogenic Na+- cotransporter in mammalian and amphibian kidney

reabsorption in the renal proximal tubule (PT). An electrogenic Na-HCO3 2 cotransporter (NBC) has recently been cloned from salamander and rat kidney. In the present study, we generated polyclonal antibodies (pAbs) to NBC and used them to characterize NBC on the protein level by immunochemical methods. We generated pAbs in guinea pigs and rabbits by immunizing with a fusion protein containing the carboxy-terminal 108 amino acids (amino acids 928–1035) of rat kidney NBC (rkNBC). By indirect immunofluorescence microscopy, the pAbs strongly labeled HEK-293 cells transiently expressing NBC, but not in untransfected cells. By immunoblotting, the pAbs recognized a ,130-kDa band in Xenopus laevis oocytes expressing rkNBC, but not in control oocytes injected with water or cRNA for the Cl/HCO3 2
Changes in composition of royal jelly harvested at di ﬀ erent times: consequences for quality standards*

– Most of the studies on royal jelly (RJ) composition or properties as well as quality standards of commercially available royal jelly are based on RJ harvested three days (72 h) after grafting. In China, some beekeepers produce RJ harvested one (24 h) or two (48 h) days after grafting. There is a lack of knowledge about the quality of the royal jelly harvested earlier than 72 h. This study compared 32 colonies for their chemical compositions of RJ harvested at 24, 48 and 72 h after grafting, according to the proportion of moisture, protein, 10-HDA, total sugar and the value of acidity and superoxide dismutase activity. The analysis of RJ samples revealed that the composition varied signiﬁcantly (for both fresh and dehydrated samples) and on some occasions above and below the range of present Chinese and Swiss standards. The results suggest that harvesting time should be considered when deﬁning new quality standards of RJ. multivariate statistics discriminant analyses were used to compare the composition, acidity and SOD activity proﬁle of the RJ harvested on di ﬀ erent days on wet and dry basis. Samples with missing values for one or more RJ components were removed from these analyses. The rela-tive amounts of the compounds were restandardised to 100% and transformed using Aitchison’s formula
Pancreatic Surgery in the Aged Population: Technical and Molecular Insights

Pancreatic resection is the only potentially curable treatment for pancreatic malignancies. Most of these malignancies occur in the aged population. In the past, these procedures were associated with significant morbidity and mortality. We herein reviewed recent advances in the knowledge of the physiological and immunological aspects of the aging process and improvements in the surgical techniques that have changed this scenario. We conclude that with appropriate selection, aged patients will outcomes similar to those of young patients, even after major pancreatic surgery. systemic inflammation characterized by organ-specific alterations to the morbidity in the aged population. that in a rodent model of acute pancreatitis, intestinal damage was much more severe in aged animals and was associated with increased bacterial translocation organ-specific alterations in an experimental model of lung injury under conditions of systemic inflammation, in which the alterations were more severe in aged animals. The aged animals exhibited higher inducible nitric oxide synthase expression and lower extracellular superoxide dismutase expression did young animals
Serum biochemistry in experimentally induced sudden death syndrome in broiler chickens

In the present study, a total of eighteen different farms with the history of sudden death syndrome (SDS) were investigated. Analysis of data revealed that the susceptibility age of sudden death syndrome was found to be as early as first week and age between 4 to 5weeks highly susceptible. The average SDS mortality percentage in 18 broiler farms ranged from 0.61 to 2.49 per cent. Clinical signs, gross and histopathological changes have been recorded from field cases. SDS was induced by administration of lactic acid through crop intubation. Clinical signs observed included loss of balance, abruptly falling on to the ground, violent wing-flapping, strong muscular contractions and extended neck and legs. Majority of affected broilers were found dead lying on their backs. Serum sodium levels in the SDS birds were significantly decreased in treatment group birds. Potassium, significantly
IMPROVEMENT OF PLASMA TREATMENT EFFICIENCY OF WOOD AND COATING PROCESS BY SODIUM CHLORIDE AQUEOUS SOLUTIONS

: The results of this study showed that the appearance of discharge during treatment of wood with plasma highly depends on density distribution along the cross section of wood. By increasing the distance between the insulated high voltage electrode and substrate´s surface, the plasma streamers become more and more present in the regions of denser latewood, and at the some point these completely disappear. Modification of wood with differently concentrated NaCl aqueous solutions lead to an increased availability of ions, which improved the hydrophilic nature of wood surfaces and enhanced the effect of treatment of wood surface with plasma, making them even more susceptible to interact with water. However, the modification of wood with salt negatively affected on adhesion strength of applied commercial water-based coating. Further experimental work is planned to determine other dielectric properties of wood modified with NaCl, as well as detailed diagnostics of the discharges during plasma treatment.
Resistance status of cultivated potatoes to early blight ( Alternaria solani ) in Denmark

Field experiments were conducted in 2015 and 2016 to classify the resistance level to early blight of the main potato cultivars in Denmark. A total of 38 cultivars were used. The experiment was designed as a randomized complete block design (RCBD) with four replicates. The potatoes were inoculated by spreading barley ( Hordeum vulgare ) kernels infested with Alternaria solani and A. alternata . Disease assessment was done at weekly intervals starting from the onset of the ﬁrst symptoms. The disease progress curves (DPC) of early blight on the cultivars were compared to that of Bintje. A multivariate analysis was performed with the apparent infection rate, the relative area under the disease progress curve, the time to reach 50% severity, the daily defoliation, the disease severity in the middle of the epidemic and the duration of the epidemic to classify the resistance level of the cultivars. The analysis of the DPC of the cultivars showed that none of the cultivars was completely resistant to early blight. However, the epidemic development differed between the different cultivars. The resistance levels of the cultivars were thus classiﬁed into classes I, II, III and IV interpreted as ‘very susceptible’, ‘susceptible’, ‘moderately slow blighting’ and ‘slow blighting’, respectively. All the late maturity cultivars were classiﬁed as class IV, and the earlier maturing cultivars were either class I or class II.
PhosphorylationInteractions May Persist beyond LAT RI e Kinetic Proofreading of Ligand-Fc

Cells may discriminate among ligands with different dwell times for receptor binding through a mechanism called kinetic proofreading in which the formation of an activated receptor complex requires a progression of events that is aborted if the ligand dissociates before completion. This mechanism explains how, at equivalent levels of receptor occupancy, a rapidly dissociating ligand can be less effective than a more slowly dissociating analog at generating distal cellular responses. Simple mathematical models predict that kinetic proofreading is limited to the initial complex; once the signal passes to second messengers, the dwell time no longer regulates the signal. This suggests that an assay for kinetic proofreading might be used to determine which activation events occur within the initial signaling complex. In signaling through the high afﬁnity IgE receptor Fc (cid:1) RI, the transmembrane adaptor called linker for activation of T cells (LAT) is thought to nucleate a distinct secondary complex. Experiments in which the concentrations of two ligands with different dwell times are adjusted to equalize the level of LAT phosphorylation in rat basophilic leukemia 2H3 cells show that Erk2 phosphorylation, intracellular Ca 2 (cid:2) , and degranulation exhibit kinetic proofreading downstream of LAT phosphorylation. These results suggest that ligand-bound Fc (cid:1) RI and LAT form a complex that is required for effective signal transmission. The Journal of Immunology, 2007, 178: 3530–3535. polyacrylamide accurate quantiﬁcation as previously (22). in- creasing amounts of immunoprecipitated Syk.