Miracle-dz/UniFAR-FTUs huggingface dataset:
Elevated BMI is considerably associated with IDD rather than polymorphic variations in interleukin-1 and vitamin D receptor genes: A case-control study

Background Intervertebral disc degeneration (IDD) is a musculoskeletal disorder and one of the major causes of low back pain leading to the disability with high economic repercussions worldwide. This study applied the candidategene approach to investigate the potential association of selected polymorphisms with IDD development in a Jordanian population. Methods MRI-diagnosed IDD patients (N=155) and asymptomatic individuals as a control group (N=55). Whole blood samples for four variants in three genes (rs1800587 of IL-1α, rs1143634 of IL-1β and rs2228570 and rs731236 of VDR) were genotyped by PCR-RFLP. Results There was no significant association between the studied polymorphisms or their allelic frequency and the occurrence of IDD. However, the cohort presented a significant reverse association between rs1143634 C > T of the IL-1β gene and the occurrence of IDD (p<0.0001). In addition, BMI showed a significant association with the IDD in the study population (p<0.005). The current study was conceptualized based on the candidate-gene approach to investigate the role of inflammatory and metabolic genes, IL and VDR, respectively, in the occurrence of IDD. Conclusions While the data presented in this study showed that polymorphisms in these genes were not associated with IDD of the cohort investigated, elevated BMI, as a measure of obesity, is strongly associated with IDD. Investigating potential roles of other structural genes, such as col-IX and aggrecan (ACAN), in IDD and considering a GWAS to elucidate a genomically global look at the basis of IDD development would be of considerable impact on our understanding of IDD.
Vitamin D receptor (BsmI) genotypes influence inflammatory and oxidative stress responses to altered vitamin D intake in subjects with Type 2 diabetes: A randomized controlled trial

B a ckground: The purpose of our study was to investigate the possible effect of Bsm I vitamin D receptor (VDR’s) polymorphism on changes of the inflammatory and oxidative stress (OS) biomarkers in response to daily intake of vitamin D- fortified yogurt drink ( doogh ) in subjects with type 2 diabetes (T2D). M ethods : In a randomized controlled trial, 100 T2D subjects were allocated to two groups to receive either plain doogh (PD; n = 50, containing 170 mg calcium and no vitamin D/250 ml) or vitamin D3-fortified doog h (FD; n = 50, containing 170 mg calcium and 500 IU/250 ml) twice a day for 12 weeks. 25(OH)D, glycemic status, inflammatory as well as OS biomarkers were evaluated before and after the intervention. VDR- Bsm I genotypes in an extended number of T2D subjects in the FD group (n = 140) were determined as BB, Bb, and bb. Results : After 12 weeks, in FD compared to PD, serum 25(OH)D increased (+30.0 nmol/l vs. −4.8 nmol/l, p < 0.001). The 25(OH)D, parathyroid hormone, highly sensitive C-reactive protein (hs-CRP), malondialdehyde (MDA), and glutathione (GSH) in patients with the BB genotype were more responsive to vitamin D intake in which the maximum increment in 25(OH)D was in BB (31.8 nmol/l) compared with Bb (24.4 nmol/l) and bb (20.8 nmol/l) (p for trend < 0.001), and the difference in BB, compared to Bb and bb was significant [Bb (p = 0.024) and bb (p = 0.037)]. This difference was accompanied by a significant difference for other biomarkers including fasting serum glucose (p trend = 0.039), fat mass % (p trend < 0.001), interleukin 6 (p trend = 0.033), hs-CRP (p trend < 0.001), and MDA (p trend = 0.028) which significantly all decreased in BB genotype except for GSH which significantly increased (p trend = 0.035). Conclusion : Daily intake of vitamin D-FD for 12 weeks improved the inflammatory and OS biomarkers in T2D subjects, and the improvement was more pronounced in the carriers of BB genotype of VDR- Bsm .
Autophagy is a double-edged sword in the therapy of colorectal cancer

Colorectal cancer is one of the leading causes of cancer-associated mortality worldwide. The limitations of colorectal cancer treatment include various types of multidrug resistance and the contingent damage to neighboring normal cells caused by chemotherapy. Macroautophagy/autophagy and apoptosis are essential mechanisms involved in cancer cell regulation of chemotherapy. Autophagy can either cause cancer cell death or promote tumor survival during colorectal cancer. Given that autophagy is involved in chemotherapy of colorectal cancer, an improved insight into the potential interactions between apoptosis and autophagy is crucial. The present review aimed to summarize the involvement of autophagy in the regulation of colorectal cancer and its association with chemotherapy. Furthermore, the role of natural product extraction, novel chemicals and small molecules, as well as radiation, which induce autophagy in colorectal cancer cells, were reviewed. Finally, the present review aimed to provide an outlook for the regulation of autophagy as a novel approach to the treatment of cancer, particularly chemotherapy-resistant colorectal cancer.
The Gendered Politics of Pandemic Relief: Labor and Family Policies in Denmark, Germany, and the United States During COVID-19

The COVID-19 pandemic has magnified families’ struggles to reconcile caregiving and employment, especially for working mothers. How have different countries reacted to these troubling circumstances? What policies have been implemented to alleviate the pernicious effects of the pandemic on gender and labor inequalities? We examine the policies offered in Denmark, Germany, and the United States, three countries that represent distinct welfare regimes. We find important differences among the policy solutions provided, but also in the “cultural infrastructures” that allow policies to work as intended, or not. In Denmark, a social-democratic welfare state, robust federal salary guarantee programs supplemented an already strong social safety net. The country was among the first to lock down and reorganize health care—and also among the first to reopen schools and child care facilities, acknowledging that parents’ employment depends on child care provisioning, especially for mothers. Germany, a corporatist regime, substantially expanded existing programs and provided generous subsidies. However, despite an ongoing official commitment to reduce gender inequality, the cultural legacy of a father breadwinner/mother caregiver family model meant that reopening child care facilities was not a first priority, which pushed many mothers out of paid work. In the U.S. liberal regime, private organizations—particularly in privileged economic sectors—are the ones primarily offering supports to working parents. Patchwork efforts at lockdown and reopening have meant a lengthy period of limbo for working families, with disastrous consequences for women, especially the most vulnerable. Among such varied “solutions” to the consequences of the pandemic, those of liberal regimes seem to be worsening inequalities. The unprecedented nature of the current pandemic recession suggests a need for scholars to gender the study of economic crises.
Longer antibiotic durations for treating peritoneal dialysis-associated peritonitis: helpful or harmful?

Abstract Relapsing, recurrent or repeat peritonitis is a devastating complication for peritoneal dialysis (PD) patients and is usually associated with poor outcomes including prolonged hospitalization, catheter removal, hemodialysis transfer and even death. Despite its critical importance and frequent occurrence, there is limited available evidence to facilitate evidence-informed treatment of PD peritonitis. This editorial comments on the findings and limitations of a randomized controlled study published in this journal, which reported that extending antibiotic treatment duration for an additional week beyond that recommended by the International Society for PD did not reduce the risk of relapsing, recurrent or repeat peritonitis, and may have increased the risk of repeat peritonitis. These results are explored in the context of the existing literature and recommendations for practice and research are provided.
A 34-Year-Old Woman with a Diamniotic Dichorionic Twin Pregnancy Presenting with an Erythematous and Papular Skin Rash Associated with SARS-CoV-2 Infection

Patient: Female, 34-year-old Final Diagnosis: COVID-19 • Pregnacy Symptoms: Earache • fatigue • odynophagia Medication: — Clinical Procedure: Cesarean Specialty: Infectious Diseases • Microbiology and Virology • Obstetrics and Gynecology Objective: Rare co-existance of disease or pathology Background: The damage caused by the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has been extensive. Pregnant women are a group requiring special attention in medicine given the anatomical and physiological changes that occur during pregnancy. Skin rash is commonly associated with pregnancy, with the most common form of an erythematous maculopapular rash being pruritic urticarial papules and plaques of pregnancy. Skin rash is also an increasingly reported initial presentation in patients with coronavirus disease 2019 (COVID-19), due to infection with SARS-CoV-2. Case Report: A 34-year-old woman with a diamniotic dichorionic twin pregnancy presented with clinical picture characterized by dermatological manifestations, namely an erythematous and papular skin rash associated with SARSCoV-2 infection. A real-time reverse transcription-polymerase chain reaction (GeneFinder) test was positive for SARS-CoV-2 detection. Conclusions: Ten months after the onset of this pandemic, there is no conclusive evidence indicating that pregnant women represent a sector more or less vulnerable to severe forms of COVID-19 than the general population. This report has highlighted the importance of performing a reliable diagnostic test for SARS-CoV-2 infection in patients who present with a skin rash, particularly pregnant women.
Regulation of Superoxide by BAP31 through Its Effect on p22phox and Keap1/Nrf2/HO-1 Signaling Pathway in Microglia

Reactive oxygen species (ROS) production by activation of microglia is considered to be a major cause of neuronal dysfunction, which can lead to damage and death through direct oxidative damage to neuronal macromolecules or derangement of neuronal redox signaling circuits. BAP31, an integral ER membrane protein, has been defined as a regulatory molecule in the CNS. Our latest studies have found that BAP31 deficiency leads to activation of microglia. In this study, we discovered that BAP31 deficiency upregulated LPS-induced superoxide anion production in BV2 cells and mice by upregulating the expression level of p22phox and by inhibiting the activation of Nrf2-HO-1 signaling. Knockdown of p22phox/keap1 or use of an NADPH oxidase inhibitor (apocynin) reversed the production of superoxide anion and inflammatory cytokines, which then reduced neuronal damage and death in vitro and in vivo. These results suggest that BAP31 deficiency contributes to microglia-related superoxide anion production and neuroinflammation through p22phox and keap1. Furthermore, the excess superoxide anion cooperated with inflammatory cytokines to induce the damage and death of neurons. Thus, we determined that BAP31 is an important regulator in superoxide anion production and neuroinflammation, and the downstream regulators or agonists of BAP31 could therefore be considered as potential therapeutic targets in microglial-related superoxide anion production and neuroinflammation.
Machine Learning for COVID-19 Diagnosis and Prognostication: Lessons for Amplifying the Signal While Reducing the Noise

S the emergence of COVID-19, researchers in machine learning and radiology have rushed to develop algorithms that could assist with diagnosis, triage, and management of the disease (1). As a result, thousands of diagnostic and prognostic models using chest radiographs and CT have been developed. However, with no standardized approach to development or evaluation, it is difficult, even for experts, to determine which models may be of most clinical benefit. Here, we share our main concerns and present some possible solutions.
Exploring Patient Safety Culture in a Kuwaiti Secondary Care Setting

Objectives Qualitative studies can improve understanding of patient safety culture (PSC), which has been relatively neglected by researchers in the Gulf Cooperation Council context. This study employed a qualitative approach to explore healthcare staff and patients’ perceptions of PSC and how it can be improved. Methods This qualitative study was conducted in a public hospital in Kuwait. Individual face-to-face interviews were used to understand the experience of healthcare staff and patients concerning PSC. After obtaining the required ethical approvals, maximum variation sampling was used. Interviews were recorded and transcribed. The analysis was inductive and thematic. Results A total of 51 participants were included in this study (35 healthcare professionals and 16 patients). Data analysis revealed four overarching themes relevant to the research question: (1) workload; (2) communication; (3) environmental constraints; and (4) incident reporting. These issues were interrelated in practice. Kuwaiti and non-Kuwaiti participants held different views, particularly about the response to errors and expatriate staff members’ clinical skills. Conclusion This study revealed multiple factors related to workload, communication, healthcare environment and incident reporting, which hindered the promotion of positive PSC in the included department. The presence of numerous constraints suggests that multiple interventions which target both individual and organisational levels should be implemented.
Impact of work environment perceptions and communication satisfaction on the intention to quit: an empirical analysis of nurses in Saudi Arabia

Objective In consideration of the current nursing shortage in Saudi Arabia, we aimed to investigate the association among perceptions of work environment, communication satisfaction, and intentions to quit nursing profession among nurses. In addition, we aimed to investigate the mediating effect of communication satisfaction on the association between nurses’ perception of work environment and their intentions to quit nursing profession. Methods This predictive correlational study was conducted at one of the major hospitals in Saudi Arabia from January 2020 to March 2020. It included a convenience sample of 367 full-time registered nurses who completed three types of close-ended questionnaires. We used IBM SPSS version 24.0 to analyze the collected data. Regression analyses were used to test the study’s hypotheses. All regression assumptions were assessed and confirmed. Significance for all tests was set at p ≤ . 05. Results The findings indicated an affirmative association between work environment perception and communication satisfaction (b = .764, p < .05) among nurses. In addition, findings showed that work environment perception (b = −.187, p < .05) and communication satisfaction (b = −.226, p < .05) have negative impacts on the nurses’ intentions to quit; indicating that as work environment perception or communication satisfaction increases, the intention to quit decreases among nurses. Further, a mediation effect of communication satisfaction on the relationship between work environment perception and intention to quit was confirmed. Conclusion This study presents a novel conceptual framework developed based on the literature about the predisposing factors for nurses’ intentions to quit nursing profession. Our results suggest that work environment perception and communication satisfaction among the most contributing factors for nurses resignation. Effective communication was established as a crucial factor for establishing attractive and healthy working environment. Nursing managers can benefit by applying these findings to develop appropriate strategies to inhibit the shortage of nurses in Saudi Arabia.
An Exploratory Study of Training Intensity in EEG Neurofeedback

Neurofeedback training has shown benefits in clinical treatment and behavioral performance enhancement. Despite the wide range of applications, no consensus has been reached about the optimal training schedule. In this work, an EEG neurofeedback practical experiment was conducted aimed at investigating the effects of training intensity on the enhancement of the amplitude in the individual upper alpha band. We designed INTENSIVE and SPARSE training modalities, which differed regarding three essential aspects of training intensity: the number of sessions, the duration of a session, and the interval between sessions. Nine participants in the INTENSIVE group completed 4 sessions with 37.5 minutes each during consecutive days, while nine participants in the SPARSE group performed 6 sessions of 25 minutes spread over approximately 3 weeks. As a result, regarding the short-term effects, the upper alpha band amplitude change within sessions did not significantly differ between the two groups. Nonetheless, only the INTENSIVE group showed a significant increase in the upper alpha band amplitude. However, for the sustained effects across sessions, none of the groups showed significant changes in the upper alpha band amplitude across the whole course of training. The findings suggest that the progression within session is favored by the intensive design. Therefore, based on these findings, it is proposed that training intensity influences EEG self-regulation within sessions. Further investigations are needed to isolate different aspects of training intensity and effectively confirm if one modality globally outperforms the other.
Clusteromics I: Principles, Protocols, and Applications to Sulfuric Acid–Base Cluster Formation

We recently coined the term clusteromics as a holistic approach for obtaining insight into the chemical complexity of atmospheric molecular cluster formation and at the same time providing the foundation for thermochemical databases that can be utilized for developing machine learning models. Here, we present the first paper in the series that applies state-of-the-art computational methods to study multicomponent (SA)0–2(base)0–2 clusters, with SA = sulfuric acid and base = [ammonia (A), methylamine (MA), dimethylamine (DMA), trimethylamine (TMA), and ethylenediamine (EDA)] with all combinations of the five bases. The initial cluster configurations are obtained using the ABCluster program and the number of relevant configurations are reduced based on PM7 and ωB97X-D/6-31++G(d,p) calculations. Thermochemical parameters are calculated based on the ωB97X-D/6-31++G(d,p) cluster structures and vibrational frequencies using the quasi-harmonic approximation. The single-point energies are refined with a high-level DLPNO-CCSD(T0)/aug-cc-pVTZ calculation. Using the calculated thermochemical data, we perform kinetics simulations to evaluate the potential of these small (SA)0–2(base)0–2 clusters to grow into larger cluster sizes. In all cases we find that having more than one type of base molecule present in the cluster will increase the potential for forming larger clusters primarily due to the increased available vapor concentration.
Singapore’s Extreme Neoliberalism and the COVID Outbreak: Culturally Centering Voices of Low-Wage Migrant Workers

I draw on the key tenets of the culture-centered approach to co-construct the everyday negotiations of COVID-19 (coronavirus disease 2019) among low-wage male Bangladeshi migrant workers in Singapore. The culture-centered approach foregrounds voices infrastructures at the margins as the basis for theorizing health. Based on 87 hours of participant observations of digital spaces and 47 in-depth interviews, I attend to the exploitative conditions of migrant work that constitute the COVID-19 outbreak in the dormitories housing low-wage migrant workers. These exploitative conditions are intertwined with authoritarian techniques of repression deployed by the state that criminalize worker collectivization and erase worker voices. The principle of academic–worker–activist solidarity offers a register for alternative imaginaries of health that intervene directly in Singapore’s extreme neoliberalism.
In Vitro Study of Octacalcium Phosphate Behavior in Different Model Solutions

Octacalcium phosphate (OCP), a new-generation bone substitute material, is a considered precursor of the biological bone apatite. The two-layered structure of OCP contains the apatitic and hydrated layers and is intensively involved in ion-exchange surface reactions, which results in OCP hydrolysis to hydroxyapatite and adsorption of ions or molecular groups presented in the environment. During various in vitro procedures, such as biomaterial solubility, additive release studies, or the functionalization technique, several model solutions are applied. The composition of the environmental solution affects the degree and rate of OCP hydrolysis, its surface reactivity, and further in vitro and in vivo properties. The performed study was aimed to track the structural changes of OCP-based materials while treating in the most popular model solutions of pH values 7.2–7.4: simulated body fluid (SBF), Dulbecco’s phosphate-buffered saline (DPBS), supersaturated calcification solution (SCS), normal saline (NS), and Dulbecco’s modified Eagle’s medium (DMEM). Various degrees of OCP hydrolysis and/or precipitate formation were achieved through soaking initial OCP granules in the model solutions. Detailed data of X-ray diffraction, Fourier-transform infrared spectroscopy, atomic emission spectrometry with inductively coupled plasma, and scanning electron microscopy are presented. Cultivation of osteosarcoma cells was implemented on OCP pre-treated in DMEM for 1–28 days. It was shown that NS mostly degraded the OCP structure. DPBS slightly changed the OCP structure during the first treatment term, and during further terms, the crystals got thinner and OCP hydrolysis took place. Treatment in SBF and SCS caused the precipitate formation along with OCP hydrolysis, with a larger contribution of SCS solution to precipitation. Pre-treating in DMEM enhanced the cytocompatibility of materials. As a result, on performing the in vitro procedures, careful selection of the contact solution should be made to avoid the changes in materials structure and properties and get adequate results.
Assessment of Geochemical Limitations to Utilizing CO2 as a Cushion Gas in Compressed Energy Storage Systems

Compressed energy storage (CES) of air, CO2, or H2 in porous formations is a promising means of energy storage to abate the intermittency of renewable energy production. During operation, gas is injected during times of excess energy production and extracted during excess demands to drive turbines. Storage in saline aquifers using CO2 as a cushion or working gas has numerous advantages over typical air storage in caverns. However, interactions between CO2 and saline aquifers may result in potential operational limitations and have not been considered. This work utilizes reactive transport simulations to evaluate the geochemical reactions that occur during injection and extraction operational cycles for CES in a porous formation using CO2 as a cushion gas. Simulation results are compared with similar simulations considering an injection-only flow regime of geologic CO2 storage. Once injected, CO2 creates conditions favorable for dissolution of carbonate and aluminosilicate minerals. However, the dissolution extent is limited in the cyclic flow regime where significantly smaller dissolution occurs after the first cycle such that CO2 is a viable choice of cushion gas. In the injection-only flow regime, larger extents of dissolution occur as the fluid continues to be undersaturated with respect to formation minerals throughout the study period and porosity increased uniformly from 24.84% to 33.6% throughout the simulation domain. For the cyclic flow conditions, porosity increases nonuniformly to 31.1% and 25.8% closest and furthest from the injection well, respectively.