Miracle-dz/UniFAR-FTUs huggingface dataset:
Climate‐Smart Land and Water Management for Sustainable Agriculture

Indian agriculture is vulnerable to climate variability and change. The Coupled Model Inter‐comparison Project Phase 5 (CMIP5) based model ensemble projects a warming of 1.5–4.3°C and an increase in precipitation by 6–14% for India under different representative concentration pathways (RCPs) for the 2080s (2071–2100) compared to the baseline period (1961–1990). The Indian Council of Agricultural Research through its network project on National Innovations on Climate Resilient Agriculture (NICRA) has demonstrated different land and water management interventions in the vulnerable districts of India to enhance the resilience of agriculture to climate change and variability. Rainwater harvesting and its judicious utilization through farm ponds and community tanks in rainfed districts demonstrated the ability to build resilience and also enhance cropping intensity (20–135%). Construction of temporary check dams (bori‐bandhan) in high‐rainfall areas brought lowland areas into cultivation which otherwise would have remained fallow due to inundation in the kharif (monsoon) season. Renovation of aahar (water reservoirs) and construction of farm ponds in Bihar ensured protective irrigation during dry spells in kharif, increasing paddy productivity by 20.7%, and also raised the groundwater level by 30 cm. Adoption of participatory integrated watershed management within a river basin perspective provides a framework for climate change adaptation through reduced runoff, increased groundwater recharge, improved crop productivity and increased carbon sequestration. Copyright © 2017 John Wiley & Sons, Ltd.
The Inhibitive and Adsorptive Characteristics of Orange Peel Extract on Metal in Acidic Media

Article history: Received 2 October 2019 Received in revised form 10 October 2019 Accepted 12 October 2019 Available online 30 December 2019 The potential of orange peel extract (OPE) to inhibit corrosion is investigated to replace the current toxic inhibitors. OPE contains inhibitor molecules that are able to adsorb onto the metal surface and form a protective film, restricting the corrosion attack. In this research, a screening between ethanol and distilled water was conducted for the selection of the best extracting solvent for orange peel extraction. It was found that ethanol extracted more functional groups and enhanced the inhibitive efficiency of OPE as compared to distilled water. The inhibitive performance of OPE was further studied with four parameters, which were orange peel to solvent ratio (0.5:100 – 10:100), contact time (0.5 hr – 3 hr), temperature (30°C – 60°C), and acid concentration (0.1 M – 10 M). It was found that the optimum conditions for the inhibition effects were at orange peel to solvent ratio of 10:100, contact time of 2 hr, temperature of 30°C at acid concentration not more than 1 M. Through the characterization studies, the properties and characteristics of inhibitor molecules in OPE responsible for adsorption were identified through FTIR and phytochemical analysis. FTIR scanning revealed that the OPE contained abundant oxygen containing groups and the functional groups contained heteroatoms that have tendency to donate their unpaired electrons to the metal surface. This could explain the formation of protective film via the adsorption of the inhibitor molecules onto the metal surface, thus reducing corrosion attack. The presence of flavonoids, saponins and phenols was also detected in OPE by conducting phytochemical analysis. These phytochemicals contained heterosides structures that have antioxidant properties which could enhance the adsorption tendency. The experimental data were analyzed by Langmuir, Freundlich, and Temkin isotherm models. The results had shown that the adsorption of OPE obeyed Langmuir adsorption isotherm model with the highest regression coefficient value (R = 0.9973). The findings in this research showed that OPE demonstrated the adsorptive and inhibitive characteristics on mild steel in acidic media, suggesting it as a potential green and sustainable option in corrosion inhibition.
Molecular detection and confirmation of in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Molecular detection and confirmation of in urogenital and extragenital specimens using the Abbott CT/NG RealTime assay and an in-house assay targeting the porA pseudogene A. Walsh, F. O. Rourke, B. Crowley
Prenatal Assessment of Genomes and Exomes Consortium (2019). Prenatal exome sequencing analysis in fetal structural anomalies detected by ultrasonography (PAGE): A cohort study. Obstetrical and Gynecological Survey , 74 (7), 394-396.

Background Fetal structural anomalies, which are detected by ultrasonography, have a range of genetic causes, including chromosomal aneuploidy, copy number variations (CNVs; which are detectable by chromosomal microarrays), and pathogenic sequence variants in developmental genes. Testing for aneuploidy and CNVs is routine during the investigation of fetal structural anomalies, but there is little information on the clinical usefulness of genome-wide next-generation sequencing in the prenatal setting. We therefore aimed to evaluate the proportion of fetuses with structural abnormalities that had identifiable variants in genes associated with developmental disorders when assessed with whole-exome sequencing (WES). In this prospective cohort study, two groups in Birmingham and London recruited patients from 34 fetal medicine units in England and Scotland. We used whole-exome sequencing (WES) to evaluate the presence of genetic variants in developmental disorder genes (diagnostic genetic variants) in a cohort of fetuses with structural anomalies and samples from their parents, after exclusion of aneuploidy and large CNVs. Women were eligible for inclusion if they were undergoing invasive testing for identified nuchal translucency or structural anomalies in their fetus, as detected by ultrasound after 11 weeks of gestation. The partners of these women also had to consent to participate. Sequencing results were interpreted with a targeted virtual gene panel for developmental disorders that comprised 1628 genes. Genetic results related to fetal structural anomaly phenotypes were then validated and reported postnatally. The primary endpoint, which was assessed in all fetuses, was the detection of diagnostic genetic variants considered to have caused the fetal developmental anomaly. in the first trimester (in three [3∙2%] of 93 fetuses). Interpretation WES facilitates genetic diagnosis of fetal structural anomalies, which enables more accurate predictions of fetal prognosis and risk of recurrence in future pregnancies. However, the overall detection of diagnostic genetic variants in a prospectively ascertained cohort with a broad range of fetal structural anomalies is lower than that suggested by previous smaller-scale studies of fewer phenotypes. WES improved the identification of genetic disorders in fetuses with structural abnormalities; however, before clinical implementation, careful consideration should be given to case selection to maximise clinical usefulness.
Integrin α 6 β 1 Is the Main Receptor for Vascular Laminins and Plays a Role in Platelet Adhesion, Activation, and Arterial Thrombosis

α 5 β 2 γ 1 ), but not laminin-211 ( α 2 β 1 γ 1 ), allow efficient platelet adhesion and activation across a wide range of arterial wall shear rates. Adhesion was critically dependent on integrin α 6 β 1 and the glycoprotein Ib-IX complex, which binds to plasmatic von Willebrand factor adsorbed on laminins. Glycoprotein VI did not participate in the adhesive process but mediated platelet activation induced by α 5 -containing laminins. To address the significance of platelet/laminin interactions in vivo, we developed a platelet-specific knockout of integrin α 6 . Platelets from these mice failed to adhere to laminin-411, laminin-511, and laminin-521 but responded normally to a series of agonists. α 6 β 1 -Deficient mice presented a marked decrease in arterial thrombosis in 3 models of injury of the carotid, aorta, and mesenteric arterioles. The tail bleeding time and blood loss remained unaltered, indicating normal hemostasis. Conclusions —This study reveals an unsuspected important contribution of laminins to thrombus formation in vivo and suggests that targeting their main receptor, integrin α 6 β 1 , could represent an alternative antithrombotic strategy with a potentially low bleeding risk. ( Circulation . 2013;128:541-552.) hereafter the wild-type (WT) ITGA6 allele (PF4-Cre as controls. Mice in VWF (VWF −/− or GPVI (GPVI and their WT control of pure C57BL/6 background. All mice were anesthetized intraperitoneally with xylazine (20 mg/kg) and ketamine (100 mg/kg).
Inactivation of Glutamine Synthetase by Tabtoxinine--lactam1 EFFECTS OF SUBSTRATES AND pH

The inactivation of glutamine synthetase by tabtoxinine-fi-lactam, a phytotoxin produced by Pseudomonas syringae pv. tabaci, was shown to be irreversible. The chloroplast and cytosolic forms of the enzyme from pea leaves (Pisum sativum L.) were separated, purified, and found to be kinetically similar with K. values for glutamate of 6.7 and 43 millimolar and for ATP of 2.0 and 1.3 millimolar, respectively. Both forms were irreversibly inactivated by the toxin at equal rates. Using the chloroplast form, it was found that inactivation by tabtoxinine-,B-lactam required ATP. Glutamate and low levels of ammonia (<2 millimolar) slowed the rate of inactivation, whereas high levels of ammonia (5, 20, and 50 millimolar) accelerated it. The inactivation proceeded at a faster rate as the pH was increased from pH 6.5 to 7.5. The role which cellular compartmentalization could play in the inactivation is discussed.
DMD059055 1719..1726

The purpose of this study was to demonstrate experimentally that alterations of in vivo transporter function at the blood-brain barrier (BBB) in disease and during pharmacotherapy can be reconstructed from in vitro data based on our established pharmacoproteomic concept of reconstructing in vivo function by integrating intrinsic transport activity per transporter molecule and absolute protein expression level at the BBB. Pentylenetetrazole (PTZ)kindled and spontaneous model of epilepsy (EL) mice were used as models of chemically induced and spontaneous epilepsy, respectively. A mouse model of antiepileptic drug treatment was prepared by consecutive 5-week administration of phenytoin (PHT). Quantitative targeted absolute proteomic analysis of 31 membrane proteins showed that P-glycoprotein (P-gp/mdr1a) protein expression levels were significantly increased in brain capillaries of PTZ (129%), EL (143%), and PHT mice (192%) compared with controls. The brain-to-plasma concentration ratios (Kp brain) of P-gp/mdr1a substrate verapamil were 0.563, 0.394, 0.432, and 0.234 in control, PTZ, EL, and PHT mice, respectively. In vivo P-gp/mdr1a function at the BBB was reconstructed from the measured P-gp/mdr1a protein expression levels and intrinsic transport activity for verapamil per P-gp/mdr1a previously reported by our group. Then, the reconstructed P-gp/mdr1a functional activities were integrated with unbound fractions of verapamil in plasma and brain to reconstruct Kp brain of verapamil. In all mice, reconstructed Kp brain values agreed well with the observed values within a 1.21-fold range. These results demonstrate that altered P-gp functions at the BBB in epilepsy and during pharmacotherapy can be reconstructed from in vitro data by means of our pharmacoproteomic approach.
INSULIN PROFILING AND HISTO-PANCREATIC ARCHITECTURE IN STZ–INDUCED DIABETES MELLITUS RATS’ MODEL TREATED WITH MISTLETOE AND DRUM- STICK AQUEOUS LEAF EXTRACTS

Reports showed that antioxidants protect against disabling effects of Diabetes mellitus by mopping up free oxygen and superoxide radicals. The study is therefore aimed at investigating some ameliorative effects of Mistletoe and Drum-stick leaf extracts on STZ–induced Diabetes mellitus on the pancreatic histo-architecture and insulin profile. Fifty four (54) Wistar rats of both sexes were selected randomly into six (6) groups (G1-G6) of nine (9) animals each. The animals were acclimatized for period of two (2) weeks. Hyperglycemia was induced in twenty seven (27) overnight-fasted randomly selected rats by a single intraperitoneal administration of Streptozotocin at 70 mg/kg bw. G1 treated with 0.1 ml of phosphate buffer saline, G2 diabetic treated with Mistletoe leaf extracts only, G3 diabetic treated with Moringa leaf extracts only, G4 diabetic without treatment, G5 normoglycemic treated with Mistletoe leaf extracts only, G6 normoglycemic treated with Moringa leaf extracts only. Administration was done for six (6) weeks, animals were sacrificed by euthanasia and pancreas was harvested, fixed in 10% formol saline for H/E, Masson’s Trichrome and Von Giessen stains. A portion of pancreas was homogenized in phosphate buffer solution for estimation of pancreatic insulin concentrations using Mercodia ultra-sensitive rat insulin Elisa kit (Mercodia, Uppsala, Sweden). Mistletoe (105.22± 4.22 mg/dl) and Drum-stick (94.33± 5.62 mg/dl) significantly lowered blood glucose levels, increase in body weight and relative pancreatic weight were observed relative to the untreated diabetic rats. The Pancreatic Insulin levels of Mistletoe (3.4±0.186 μg/L) and Drum-stick (3.5±0.088 μg/L) treated hyperglycemic groups were not significantly different from normal control (3.8±0.25 μg/L) P>0.05. The pancreatic histo-architecture was maintained in the pancreatic islet of Drum-stick treated group with improved vascularization, increase in β cell mass. Elastic fibers were extensively deposited in the acini of untreated diabetic group. Thus aqueous leaf extracts of Mistletoe and Moringa maintained pancreatic islet morphology and insulin profiling.
Bootstrapped Pivots for Means of Short and Long Memory Linear Processes

Re-sampling only once, with replacement, from the entire set of observations (without dividing them into blocks) from short or long memory linear processes, we construct direct randomized parametric and nonparametric (Studentized) pivots for the population mean. In the included numerical studies, the resulting pivots will be seen to significantly outperform the traditional t-statistic as a pivot for the population mean. Our approach to the bootstrap in this exposition is fundamentally different from the classical methods of the bootstrap that have been used when the population mean is the parameter of interest. In the latter a relatively large number of independent bootstrap sub-samples are drawn from the original data to estimate the sampling distribution of the traditional t-statistic, when the latter itself is studied in reference to making inference about the population mean. Using the approach of this paper, one does not encounter the well-known issues with bootstrapped t-statistics for dependent data. Hence, no adjustments such as block-bootstrapping are required. This is so, since the re-sampling mechanism in our introduced pivots for the population mean preserves the covariance structure of the observables in hand.
Many hands lighter work? Deciphering the relationship between Adverse Working Conditions and Organization Citizenship Behaviors in SMEs during a severe economic crisis

“What is the relationship between Adverse Working Conditions (AWCs) and employees’ Organizational Citizenship Behavior (OCB) in small and medium-sized enterprises (SMEs) operating under a severe economic crisis? ” To address this question, a survey of 312 frontline workers was undertaken in 62 Greek SMEs - an instrumental setting where the current deterioration of working conditions is acute. Our contribution is twofold. First, we develop and test a scale for measuring AWCs. Second we decipher the extent to which such conditions relate to organizational and individual aspects of OCB, considering job satisfaction’s mediating role. Through this research we extend the OCB literature within the context of SMEs operating under severe economic crisis and highlight the implications for managing human resources in SMEs, a sector conspicuous for its socio-economic significance and its vulnerability during economic downturns.
Purchasing Behavior of Rural Migrants: A Comparative Analysis of Sukkur and Khairpur Districts

Pakistan is witnessing rural to urban migration because urban areas are comparatively advanced and are equipped with superior infrastructure than rural areas. This study has been designed mainly to compare the Purchasing Behavior between rural migrants of Sukkur and Khairpur districts. Primary data was collected using survey method from 383 rural migrants and data was analyzed by using Factor Analysis and Independent Samples Test. Factor Analysis results indicate that rural migrants of Sukkur and Khairpur districts while purchasing regard Brand Switchover Behavior (BSB), Purchasing Consciousness (PC), Enjoy Purchasing (EP), Income/ Pocket Money (INC/PM) and Price Consciousness/ Quality Consciousness (PRC/QC). Further results if Independent Samples Test suggested that all factors affecting Purchasing Behavior of r migrants in Sukkur district are significantly different than in Khairpur district. This study is helpful for companies and organizations as they can area difference in Purchasing Behavior of rural migrants and they can increase number of customers. ---------------------------------------------------------------------------------
heterogeneity of wellbeing “ expenditure ” preferences : evidence from a simulated allocation choice on the

With an online survey on major Italian newspapers we ask respondents to simulate the typical policymaker decision, that is, the dilemma of allocating scarce financial resources among alternative competing goals using the domains of the newly defined Italian BES (sustainable and equitable wellbeing) indicators. Our main finding is that homogeneity of choices is rejected since preferred allocations are strongly affected by socio‐demographic factors and mainly by political orientation, age, education and gender. An important related result is that education and political orientation significantly affect preferences toward sustainable development. We as well find that respondents' expenditure preferences on a given BES domain are mainly affected by the relative scarcity/abundance of wellbeing on that given domain at the regional level.
A prospective, randomised study of the results after surgical and non-surgical treatment

Only two prospective, randomised studies have been published on the outcome after treatment for Achilles tendon rupture. The controversy regarding the optimal treatment continues. In the present study, 112 patients with acute Achilles tendon rupture were randomised and all of them were followed up for two years. Fifty-nine patients were treated surgically with end-to-end sutures followed by two weeks of plaster treatment and six weeks of treatment in a brace with increasing range of motion. Fifty-three patients were treated non-surgically with four weeks of plaster in equinus and four weeks in a neutral position. The re-rupture rate was 20.8% in the non-surgical-treatment group and 1.7% in the surgical-treatment group (p=0.001). There were no major surgical complications. A new Achilles Tendon Rupture score including five objective and three subjective parameters did not reveal any significant difference between the treatment groups. The time of return to work and sports did not differ significantly between the treatment groups either. Calf muscle strength was evaluated both for purposes of test-retest reliability in healthy volunteers and for outcome reasons in the clinical study. Isokinetic torque production in concentric and eccentric muscle action in plantar flexion and dorsiflexion at the ankle joint was studied on the right and left sides. Calf muscle endurance was evaluated using a standardised heel-raise test, until fatigue. The reliability test showed acceptable reproducibility for the isokinetic tests and the endurance tests. After treatment for ATR, we found calf muscle hypotrophy, thickening of the Achilles tendon, decreased calf muscle strength and reduced endurance on the injured side throughout the study period. There were, however, no significant differences between the treatment groups. Magnetic resonance imaging and ultrasonography detected the same amount of pathological findings during healing in both treatment groups. The correlation between the radiological findings and the clinical parameters was weak. The non-surgical treatment of ATR, which produced treatment failure in every fifth patient, cannot be regarded as acceptable for healthy, active people under the age of 65 years. Surgical treatment followed by early functional rehabilitation is a safe method for the treatment of ATR with a low risk of complications. However, surgical and non-surgical treatments produced equally good medium-term results in the group of patients in whom no rerupture occurred.
Gene expression during ER stress–induced apoptosis in neurons: induction of the BH3-only protein Bbc3/PUMA and activation of the mitochondrial apoptosis pathway

ndoplasmic reticulum (ER) stress has been implicated in the pathogenesis of ischemic and neurodegenerative disorders. Treatment of human SH-SY5Y neuroblastoma cells with tunicamycin, an inhibitor of protein glycosylation, rapidly induced the expression of target genes of the unfolded protein response. However, prolonged treatment also triggered a delayed, caspase-dependent cell death. Microarray analysis of gene expression changes during tunicamycin-induced apoptosis revealed that the Bcl-2 homology domain 3-only family member, Bcl-2 binding component 3/p53 upregulated modulator of apoptosis ( Bbc3/PUMA ), was the most strongly induced pro-apoptotic gene. Expression of Bbc3/PUMA correlated with a Bcl-xL–sensitive release of cytochrome c and the activation of caspase-9 and -3. Increased expression of Bbc3/PUMA was also observed in p53-deﬁcient human cells, in response to the ER stressor thapsigargin, and in rat hippocampal neurons after transient forebrain ischemia. Overexpression of Bbc3/PUMA was sufﬁcient to trigger apoptosis in SH-SY5Y neuroblastoma cells, and human cells deﬁcient in Bbc3/PUMA showed dramatically reduced apoptosis in response to ER stress. Our data suggest that the transcriptional induction of Bbc3/ PUMA may be sufﬁcient and necessary for ER stress–induced apoptosis.
PROVIDING EARLY FOREIGN LANGUAGE EDUCATION IN VIETNAMESE CONTEXT

There is a common belief that providing early language education facilitates learners‟ language acquisition. This belief is supported by critical period hypothesis defined as a biological period that humans are set to acquire a language. However, much research has found the opposite. This paper critically discusses the evidence supporting „the younger the better‟ viewpoint and counterevidence against this belief. More importantly, it brings into concern the constraints of introducing early language education in the context of Vietnam