Miracle-dz/UniFAR-FTUs huggingface dataset:
Developmental changes of free amino acids in amniotic, allantoic fluids and yolk of broiler embryo

ABSTRACT 1. A study was conducted to evaluate the developmental changes of protein and free amino acid concentrations in amniotic, allantoic fluids and yolk during the incubation period of broiler eggs. 2. A total of 120 Cobb 500 fertile eggs were individually weighed and then placed in an incubator. On incubation day: 8, 11, 13, 14, 16, and/or 18, amniotic, allantoic fluids and yolk were collected from 20 eggs for analysis of protein content and free α-amino acid concentration in allantoic and amniotic fluids and yolk. 3. The total protein concentration in amniotic fluid increased from d 11 of incubation, and reached a peak at d 16 (69.85 g/l; P < 0.01), then declined at d 18 (P < 0.05). The total protein concentration in allantoic fluid increased with age of the embryo (P < 0.01). Crude protein concentration in yolk decreased (P < 0.05) from d 0 to 8, then increased gradually from d 8, and reached a peak at d 16 (P < 0.05). The concentration of most free amino acids in amniotic and allantoic fluids and yolk was related to embryo weight. Amniotic fluid amino acids gradually increased from d 13 to 18, with arginine being the most abundant at d 11 and 14. Glutamate was the most predominant amniotic fluid amino acid at d 16 and 18. From d 13 to 18, the concentrations of most α-amino acids in allantoic fluid increased, and reached a peak at d 18 (aspartate, 373 μmol/l; asparagine, 519 μmol/l; glutamine, 1230 μmol/l; threonine, 537 μmol/l; citrulline, 112 μmol/l; arginine, 2747 μmol/l; alanine, 276 μmol/l; tyrosine, 330 μmol/l; tryptophan, 212 μmol/l; valine, 140 μmol/l; phenylalanine, 102 μmol/l; isoleucine, 92.39 μmol/l; lysine, 1088 μmol/l; P < 0.05). Glutamine was the second most abundant amino acid in allantoic fluid at d 13 and 18. Glutamate was the most abundant α-amino acids at d 8, and 13 in the yolk. 4. These results demonstrated that the concentration of free α-amino acids in chicken embryo fluid was related to embryo weight. Arginine, glutamine and glutamate were abundant free α-amino acids in chicken embryo fluid, to support the higher rates of tissue protein synthesis and growth for the embryo.
Balance of osmotic pressures determines the nuclear-to-cytoplasmic volume ratio of the cell

Significance For over a century, it has been known that the ratio of the nuclear and cytoplasm volumes (NC ratio), rather than the separate volumes, is constant among cells of many types of organisms. Changes of the NC ratio are associated with cancerous transformations, suggesting that the ratio has physiological importance. Notably, the dominant regulatory mechanism of the NC ratio has not been identified. Here, we use physical estimates of the forces implicated in nuclear volume determination and show that they are dominated by the osmotic pressure of actively transported proteins. Inspired by this, we formulate a minimal model for the cytoplasmic and nuclear volumes that predicts the NC ratio and the factors that modulate it, in agreement with published experiments.
Effect of a brain‐penetrant selective estrogen receptor degrader (SERD) on binge drinking in female mice

Abstract Background Greater circulating levels of the steroid hormone 17β‐estradiol (E2) are associated with higher levels of binge drinking in women. In female mice, estrogen receptors in the ventral tegmental area, a dopaminergic region of the brain involved in the motivation to consume ethanol, regulate binge‐like ethanol intake. We recently developed a brain‐penetrant selective estrogen receptor degrader (SERD), YL3‐122, that could be used to test the behavioral role of brain estrogen receptors. We hypothesized that treating female mice with this compound would reduce binge‐like ethanol drinking. Methods Female C57BL/6J mice were treated systemically with YL3‐122 and a related SERD with low brain penetrance, XR5‐27, and tested for binge‐like ethanol consumption in the drinking in the dark (DID) test. Mice were also tested for sucrose and water consumption and blood ethanol clearance after treatment with the SERDs. Finally, the effect of ethanol exposure on Esr1 gene expression was measured in the ventral tegmental area (VTA), prefrontal cortex (PFC), and ventral hippocampus (vHPC) of male and female mice by quantitative real‐time PCR after 4 DID sessions. Results YL3‐122 reduced ethanol consumption when mice were in diestrus but not estrus. YL3‐122 also decreased sucrose consumption but did not alter water intake or blood ethanol clearance. XR5‐27 did not affect any of these measures. Binge‐like ethanol drinking resulted in increased Esr1 transcript in the VTA of both sexes, male vHPC, and female PFC. Conclusions These results indicate that SERD treatment can decrease binge‐like ethanol drinking in female mice. Thus, it could be a novel strategy to reduce binge drinking in women, with the caveat that effectiveness may depend on menstrual cycle phase. In addition, Esr1 transcript is increased by binge ethanol exposure in both sexes but in a brain region‐specific manner.
Strategies to Retain Millennial Employees at Full-Service Restaurants

Strategies to Retain Millennial Employees at Full-Service Restaurants by Candace A. Ruiz MBA, Regis University, 2007 BS, Metropolitan State University of Denver, 2004 Doctoral Study Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Business Administration Walden University February 2017 Abstract Some supervisors lack effective strategies to retain millennial generation employees. The purpose of this multicase study was to explore effective strategies supervisors use to retain culinary-educated millennial employees in full-service restaurants. Nine millennials and 3 supervisors from 3 full-service restaurants in Denver, ColoradoSome supervisors lack effective strategies to retain millennial generation employees. The purpose of this multicase study was to explore effective strategies supervisors use to retain culinary-educated millennial employees in full-service restaurants. Nine millennials and 3 supervisors from 3 full-service restaurants in Denver, Colorado consented to face-to-face semistructured interviews with open-ended interview questions concerning retention strategies. The conceptual framework of Herzberg’s two-factor theory served to guide the scope and the data analysis for the multicase study. The interview transcripts were coded and grouped into themes. Explanation building for the data analysis assisted in finding causal links between cases and the unit of analysis, and in assembling a broad explanation to fit each case. Ten subcategories emerged from the 3 themes that aligned with Herzberg’s motivation factors, hygiene factors, and millennial values and attitudes. The 10 subcategories were developed, and indicated that effective strategies differ by supervisor. Among the ten subcategories, the most prominent were (a) growth and advancement, (b) positive working conditions, and (c) quality and influence of the supervisor. This study may contribute to social change by providing restaurant supervisors with strategies for millennial retention and keeping young workers in the community to share in the responsibility of social progress and to reach their full potential. Strategies to Retain Millennial Employees at Full-Service Restaurants by Candace A. Ruiz MBA, Regis University, 2007 BS, Metropolitan State University of Denver, 2004 Doctoral Study Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Business Administration
iNOS is essential to maintain a protective Th1/Th2 response and the production of cytokines/chemokines against Schistosoma japonicum infection in rats

Humans and a wide range of mammals are generally susceptible to Schistosoma infection, while some rodents such as Rattus rats and Microtus spp are not. We previously demonstrated that inherent high expression levels of nitric oxide (NO), produced by inducible nitric oxide synthase (iNOS), plays an important role in blocking the growth and development of Schistosoma japonicum in wild-type rats. However, the potential regulatory effects of NO on the immune system and immune response to S. japonicum infection in rats are still unknown. In this study, we used iNOS-knockout (KO) rats to determine the role of iNOS-derived NO in the immune system and immunopathological responses to S. japonicum infection in rats. Our data showed that iNOS deficiency led to weakened immune activity against S. japonicum infection. This was characterized by the impaired T cell responses and a significant decrease in S. japonicum-elicited Th2/Th1 responses and cytokine and chemokine-producing capability in the infected iNOS-KO rats. Unlike iNOS-KO mice, Th1-associated cytokines were also decreased in the absence of iNOS in rats. In addition, a profile of pro-inflammatory and pro-fibrogenic cytokines was detected in serum associated with iNOS deficiency. The alterations in immune responses and cytokine patterns were correlated with a slower clearance of parasites, exacerbated granuloma formation, and fibrosis following S. japonicum infection in iNOS-KO rats. Furthermore, we have provided direct evidence that high levels of NO in rats can promote the development of pulmonary fibrosis induced by egg antigens of S. japonicum, but not inflammation, which was negatively correlated with the expression of TGF-β3. These studies are the first description of the immunological and pathological profiles in iNOS-KO rats infected with S. japonicum and demonstrate key differences between the responses found in mice. Our results significantly enhance our understanding of the immunoregulatory effects of NO on defensive and immunopathological responses in rats and the broader nature of resistance to pathogens such as S. japonicum.
Improved executive function in patients with systemic lupus erythematosus following interactive digital training

Objective This study aimed to evaluate the sensitivity of a digital platform to assess attentional and executive function in systemic lupus erythematosus (SLE) patients, and to evaluate the impact of an at-home interactive digital treatment to improve cognitive dysfunction in this clinical population. Background Deficits in attention and executive function are common in patients with SLE. Despite these cognitive difficulties, there are limited brief assessment techniques and few treatment options to improve cognitive abilities in patients with SLE. Interactive digital treatment approaches (use of video game-based software) have been successful in identifying and improving cognition in other clinical populations. Methods Sixty SLE patients completed baseline neuropsychological tests (of attention, psychomotor speed, and executive function), a tablet-based digital platform (EVOTM Monitor), and biobehavioral measures. The patients were randomized into treatment SLE (n = 30) or no contact control SLE (n = 30) groups, and returned 4 weeks later for follow-up cognitive, EVO Monitor, and biobehavioral testing. The SLE treatment group was trained on a tablet-based digital treatment (AKL-T01) and was instructed to complete 5 sessions at least 5 days per week for 4-weeks for a total of approximately 25 min of gameplay per day. Results Systemic lupus erythematosus patients demonstrated impairment in visuomotor and processing speed, visual attention, and cognitive flexibility/sequencing skills at baseline. The video game-like treatment group (AKL-T01) had significant improvement in visuomotor speed (Trail Making A) and cognitive flexibility/sequencing (Trail Making B) compared to the control group at 4-week follow-up. The treatment group also demonstrated significant improvement in EVO Monitor multitasking at follow-up (with no change in controls). At baseline, a multitasking metric from EVO Monitor was associated with performance on tasks of cognitive flexibility (Trail Making B) and psychomotor speed (WAIS-IV Coding). Conclusions These findings provide evidence that SLE patients who participated in a 4-week interactive digital video game-like activity had significant improvement in motor speed and executive functions, and would benefit from participation in digital interventions designed to target frontoparietal networks of the brain. Preliminary findings also suggest specific metrics from EVO Monitor may also be useful to detect cognitive impairment and cognitive changes in patients with SLE.
The effect of brain serotonin deficiency on breathing is magnified by age

Serotonin is an important mediator modulating behavior, metabolism, sleep, control of breathing, and upper airway function, but the role of aging in serotonin‐mediated effects has not been previously defined. Our study aimed to examine the effect of brain serotonin deficiency on breathing during sleep and metabolism in younger and older mice. We measured breathing during sleep, hypercapnic ventilatory response (HCVR), CO2 production (VCO2), and O2 consumption (VO2) in 16–18‐week old and 40–44‐week old mice with deficiency of tryptophan hydroxylase 2 (Tph2), which regulates serotonin synthesis specifically in neurons, compared to Tph2+/+ mice. As expected, aging decreased VCO2 and VO2. Tph2 knockout resulted in an increase in both metabolic indexes and no interaction between age and the genotype was observed. During wakefulness, neither age nor genotype had an effect on minute ventilation. The genotype did not affect hypercapnic sensitivity in younger mice. During sleep, Tph2−/− mice showed significant decreases in maximal inspiratory flow in NREM sleep, respiratory rate, and oxyhemoglobin saturation in REM sleep, compared to wildtype, regardless of age. Neither serotonin deficiency nor aging affected the frequency of flow limited breaths (a marker of upper airway closure) or apneas. Serotonin deficiency increased the amount and efficiency of sleep only in older animals. In conclusion, younger Tph2−/− mice were able to defend their ventilation and phenotypically did not differ from wildtype during wakefulness. In contrast, both young and old Tph2−/− mice showed sleep‐related hypoventilation, which was manifested by hypoxemia during REM sleep.
Measurement Invariance of Moral Foundations across Population Strata

Abstract A representative sample (n = 2282) of Swedish adults completed the Moral Foundations Questionnaire, which measures moral intuitions concerning care, fairness, loyalty, authority, and purity. A subset (n = 607) completed a measure of intuitions about liberty. Measurement invariance was estimated across sex, age, education, income, left-right placement, religiosity, and party preference groups, based on multigroup confirmatory factor analyses of two-, three-, five-, six-, and eight-factor models, as well as bifactor models (with methods factors or a general factor). Acceptable configural, metric, and scalar invariance was obtained for most group comparisons, particularly based on the more complex models. The clearest exceptions were (1) configural non-invariance in comparisons involving participants with very low education or income, and (2) scalar non-invariance in comparisons of ideological groups based on three- and six-factor models but not the eight-factor model, which distinguished lifestyle liberty from government liberty.
An Assessment of Solid Waste and Landfilis in Muscat Area, Oman

The paper presents the results of a 1998 study of the solid waste generation within the greater Muscat City area. The recent data were compared with the available data from the last 10 years to assess the solid waste problems within the area. Five operating landfills were used as the data collection points. It appeared that through the last decade, the solid waste generation rate has increased sharply. However, the rate of waste production stabilized to 750 – 800 ton/day during the last three years of the 1990s. The rate amounts to 1.5 kg/person/day. Overall municipal waste volume decreased through the years while the amounts generated from private sources such as industries and commercial centers increased during the same period. This is due to the fact that the Sultanate of Oman is developing very fast with increasing industrial, commercial and residential activities. About 80 % of the solid wastes is organic materials. This amount is high when compared to figures found in western countries. The data indicate that about 40 percent of the solid wastes are composed of recoverable materials. The concerned authorities should encourage the growth of industries to be involved in recycling business. Such activities will reduce the solid waste discharge in landfills and increase nearly 1.6 folds the expected life of existing landfills. A survey of the operating landfills indicate that the operational efficiency may be substantially improved by incorporating measures that deal with problems of gas production and leachate movements. The paper includes a detailed discussion of the problem and makes recommendations for possible future actions.
Activity-Based Costing Diffusion, Strategy and Organizational Performance: Evidence from Iran

While management accounting literature has shown substantial evidence on ABC adoption and implementation within the Western context, this study attempted to provide some insight into the ABC diffusion stages within the Iranian context. Using a revised four-stage model in measuring activity-based costing (ABC) diffusion, this study examines the performance consequence of ABC diffusion together with its relationship with Miles and Snow (1978) strategic types. From purposeful sampling of manufacturing companies listed in the Tehran Stock Exchange, a total of 400 survey questionnaires sent to these companies where 300 usable completed questionnaires were returned and analysed. Hypotheses were tested using ordinal logistic regression and ANOVA analyses. The results showed that firms at higher ABC diffusion stages have better levels of organizational performance than firms at the lower ABC diffusion stages. In addition, the analyzer strategy was a significant factor in determining a firm’s decision to go to higher ABC diffusion stages.
The role of Pycnogenol in the control of inflammation and oxidative stress in chronic diseases: Molecular aspects

The prevalence of chronic diseases has increased significantly with the rising trend of sedentary lifestyles, reduced physical activity, and dietary modifications in recent decades. Inflammation and oxidative stress play a key role in the pathophysiology of several chronic diseases, such as type II diabetes, cardiovascular diseases, and hepatic conditions. Therefore, reducing inflammation and oxidative stress may be beneficial in the prevention and treatment of various chronic disorders. Since chronic diseases are not completely curable, various methods have been proposed for their control. Complementary therapies and the use of natural antioxidant and antiinflammatory compounds are among these novel approaches. Pycnogenol (PYC) is a natural compound that could control inflammation and oxidative stress. Furthermore, some previous studies have shown that PYC could effectively reduce inflammation through signaling the downstream of insulin receptors, inhibiting the phosphorylation of the serine residues of insulin receptor substrate‐1, reducing pro‐inflammatory cytokines and oxidative stress indices through the stimulation of antioxidant pathways, increasing free radical scavenging activities, preventing lipid peroxidation, and protecting the erythrocytes in glucose‐6‐phosphate dehydrogenase‐deficient individuals, although these effects have not been fully proved. The present study aimed to comprehensively review the evidence concerning the positive physiological and pharmacological properties of PYC, with an emphasis on the therapeutic potential of this natural component for enhancing human health.
Older Korean Americans’ Perceived Burdensomeness to Their Healthcare Partners: An Egocentric Network Assessment

Objectives: The objective is to examine how older Korean Americans’ perceived burdensomeness to their healthcare partners is associated with the characteristics of older adult participants (egos) and their healthcare partners (alters). Methods:Surveys of 2150 participants in the Study of Older Korean Americans provided ego data. Participants were also asked to list up to three individuals whom they usually asked for help on health-related matters or healthcare use, which generated 3402 alters. Multilevel modeling was conducted to examine the role of the characteristics of egos and alters, as well as their cross-level interactions. Results: Perceived burdensomeness was negatively associated with English-speaking ability and residence in a low Korean density area. Perceived burden was also lowered when emotional support was received from the alters, and this pattern was pronounced among those with multi-comorbidity. Discussion: By identifying older immigrants prone to the sense of burdensomeness, the study suggests strategies to promote their health and healthcare use.
RELIABILITY AND VALIDITY OF A CHINESE VERSION OF THE LYSHOLM SCORE AND TEGNER ACTIVITY SCALE FOR KNEE ARTHROPLASTY

Objective To verify the reliability and validity of Chinese versions of the Lysholm score and the Tegner activity scale for knee arthroplasty. Methods Sixty-four patients undergoing total knee arthroplasty and 28 healthy volunteers were included in this study. Participants were divided into 4 groups: a pre-operation group; a 3 months post-operation group; a 1-year post-operation group; and a control group of healthy volunteers. Participants completed the Lysholm score and Tegner activity scale twice over a period of 3–7 days. Results The intraclass correlation coefficients of the Lysholm score and Tegner scale were both relatively high, at 0.99 and 0.97, respectively. Moreover, the Cronbach’s alpha of the Lysholm score was 0.71. The items “locking” and “instability” differed slightly between groups (Kruskal–Wallis: for locking, χ2 (p) = 13.48, p = 0.0037; for instability, χ2 (p) = 4.32, p = 0.2292). Conclusion The simplified-Chinese versions of the Lysholm score and the Tegner scale are applicable for use with patients undergoing total knee arthroplasty, and have relatively high validity and reliability. The items “locking” and “instability” should be combined with clinical data to make the Lysholm score more suitable for assessment of total knee arthroplasty. LAY ABSTRACT The aim of this study was to verify the reliability and validity of Chinese versions of the Lysholm score and the Tegner activity scale for assessment of knee arthroplasty. A total of 64 patients undergoing total knee arthroplasty (TKA) and 28 healthy volunteers were included in the study. Participants were divided into 4 groups: a pre-operation group; a 3 months post-operation group; a 1-year post-operation group; and a control group of healthy volunteers. Study participants completed the Lysholm score and Tegner activity scale score twice for over a period of 3–7 days, and the results analysed. The simplified-Chinese versions of the Lysholm score and the Tegner scale were found to be applicable for use with total knee arthroplasty patients, and to have relatively high validity and reliability. Furthermore, the items “locking” and “instability” should be combined with clinical data to make the Lysholm score more suitable for assessment of total knee arthroplasty.