Miracle-dz/UniFAR-FTUs huggingface dataset:
Myofibrillar Architecture in Engineered Cardiac Myocytes

Morphogenesis is often considered a function of transcriptional synchrony and the spatial limits of diffusing mitogens; however, physical constrainment by the cell microenvironment represents an additional mechanism for regulating self-assembly of subcellular structures. We asked whether myocyte shape is a distinct signal that potentiates the organization of myofibrillar arrays in cardiac muscle myocytes. We engineered the shape of neonatal rat ventricular myocytes by culturing them on microfabricated fibronectin islands, where they spread and assumed the shape of the island. Myofibrillogenesis followed, both spatially and temporally, the assembly of unique actin networks whose architecture was predictable given the shape of the island. Subsequently, the z lines of the sarcomeres aligned and registered in distinct patterns in different regions of the myocytes in such a way that orthogonal axes of contraction could be distinctly engineered. These data suggest that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization.
Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ

Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and infiltrate brain parenchyma, they closely interact with a changing micro-environmental landscape that sustains tumor cell invasion. In this study, we used a unique microarray profiling approach on a human glioma stem cell (GSC) xenograft model to explore gene expression changes in situ in Invading Glioma Cells (IGCs) compared to tumor core, as well as changes in host cells residing within the infiltrated microenvironment relative to the unaffected cortex. IGCs were found to have reduced expression of genes within the extracellular matrix compartment, and genes involved in cell adhesion, cell polarity and epithelial to mesenchymal transition (EMT) processes. The infiltrated microenvironment showed activation of wound repair and tissue remodeling networks. We confirmed by protein analysis the downregulation of EMT and polarity related genes such as CD44 and PARD3 in IGCs, and EFNB3, a tissue-remodeling agent enriched at the infiltrated microenvironment. OLIG2, a proliferation regulator and glioma progenitor cell marker upregulated in IGCs was found to function in enhancing migration and stemness of GSCs. Overall, our results unveiled a more comprehensive picture of the complex and dynamic cell autonomous and tumor-host interactive pathways of glioma invasion than has been previously demonstrated. This suggests targeting of multiple pathways at the junction of invading tumor and microenvironment as a viable option for glioma therapy.
Towards productive welfare? A comparative analysis of 23 OECD countries

Numerous analysts have suggested that globalization and the emergence of more knowledge-based economies have encouraged high-income nations to shift towards a model of productive welfare focused on social investment, yet typologies of welfare are still largely drawn on the basis of measures of social protection rather than social investment. Here we develop a classification of welfare state types that incorporates both productive and protective elements of social policy. Using fuzzy set ideal type analysis we explore data for a sample of 23 OECD countries in three time points: 1994, 1998 and 2003. Our findings provide no more than very modest support for claims that welfare states are shifting from protective to productive modes of provision and, in many cases, we identify a shift in the alternative direction. In addition, we identify some nations that are clearly productive in their focus and others that manage to combine productive and protective features.
Impact of Microclimate Modification on Okra Cultivation: A Global Review

Okra [Abelmoschus esculentus (L.) Moench] is an economically important vegetable crop grown in tropical and sub-tropical regions of the world. Okra is also locally known as Lady Finger or Bhindi and is one of the major kharif and summer season cultivated vegetable crop of India as well as Haryana. It is a member of the hibiscus family, Malvaceae and has the typical floral characteristics of that family originating from Africa. It is now widely distributed in the tropics including Nigeria (National Research Council, 2006). The cultivation of okra can be done as a garden crop as well as on large commercial farm crop and nowadays okra is also cultivated under polyhouse or green house with the use of microclimatic modification strategies in all seasons. It is grown commercially in different countries like India, Turkey, Nigeria, Sudan, Iraq, Pakistan and Egypt. Okra plays a significant role in human nutrition by providing carbohydrates, protein, fat, minerals and vitamins that are generally deficient in basic foods in the diet of developing countries (IBPGR, 1991). Okra requires a long, warm and humid growing climate for better yield. It is sensitive to frost and extremely low International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 10 (2018) Journal homepage: http://www.ijcmas. com
Counterexample guided inductive optimization applied to mobile robots path planning

We describe and evaluate a novel optimization-based off-line path planning algorithm for mobile robots based on the Counterexample-Guided Inductive Optimization (CEGIO) technique. CEGIO iteratively employs counterexamples generated from Boolean Satisfiability (SAT) and Satisfiability Modulo Theories (SMT) solvers, in order to guide the optimization process and to ensure global optimization. This paper marks the first application of CEGIO for planning mobile robot path. In particular, CEGIO has been successfully applied to obtain optimal two-dimensional paths for autonomous mobile robots using off-the-shelf SAT and SMT solvers.
Poetry in motion: appropriation of the world of apps

Motivation -- This study was motivated by an interest in understanding the new opportunities brought to use by App technologies available on mobile devices. In our qualitative analysis of interview data we used the concept of 'appropriation', and in doing so we realized that we needed to address both individual and social appropriation. Research approach is a hermeneutic interpretation of data from interviews with 12 iPhone users triangulated with models of appropriation, theories of micro and macro level appropriation, and the concept 'expansive learning' Findings/Design -- Through use, idiosyncratically and in collaboration with others, people make the iPhone and its App-world their own to the extent that they use the phone as a port to exercising personal interests like poetry, Italian novels, planning and cookbooks; hence the title of this paper. A closer look shows that in doing so, the interviewees have expanded their scope of what activity is enabled by their iPhone. Research limitations/Implications -- Despite being an explorative study addressing only Danish users of iPhones and Apps, our findings suggest to take seriously the expansion of users' scope of activity and abandon the idea that use can be predicted. Originality/Value -- This paper presents a new conceptualization of context of use. The presented analysis of data opens a window to the transitions that users undergo, alone and together in order to make the iPhone their own. A particular focus is how the iPhone and its Apps support or hinder the artefact to become a personal access-point to the world of Apps. Take away message -- The paper presents findings, which indicate that appropriation takes place at two levels, first at the social, and then at the individual level. This pattern is parallel to that of learning in general. The conclusion we draw from this finding is that expansion must be also a social process where you learn by constructing a new activity.
Mediterranean Built Environment and Weather as Modulator Factors on Physical Activity: Cross-Sectional Study

Background: When promoting physical activity practice, it is important to consider the plausible environmental determinants that may affect this practice. We aimed to explore the impact of objectively measured Public Open Spaces (POS) on objectively measured and self-reported physical activity and the influence of weather on this association, in a Mediterranean sample of senior adults with overweight or obesity and the metabolic syndrome. Method: Cross-sectional analyses based on 218 PREDIMED-Plus trial participants aged 55 to 75 years, from Palma de Mallorca (Spain). Indicators of access to POS were assessed in a 1.0 km sausage network walkable buffer around each participant’s residence address using geographic information systems. Mean daily minutes of self-reported leisure-time brisk walking, and accelerometer objectively measured moderate-to-vigorous physical activity in bouts of at least 10 min (OM-MVPA) were measured. To investigate the association between access to POS and physical activity, generalized additive models with Gaussian link function were used. Results: Better access to POS was not statistically significantly associated with self-reported leisure-time brisk walking. A positive significant association was only observed between the distance of healthy routes contained or intersected by buffer and OM-MVPA. This association was only evident on non-rainy days. Conclusions: In this elderly population living in a Mediterranean city, only healthy routes contained or intersected by a 1 km sausage network walkable buffer influenced the accelerometer objectively measured moderate-to-vigorous physical activity in bouts of at least 10 min and rainy conditions during the accelerometer period appeared to be an important factor related to active ageing.
Epigraphics: Message-Driven Infographics Authoring

The message a designer wants to convey plays a pivotal role in directing the design of an infographic, yet most authoring work-flows start with creating the visualizations or graphics first without gauging whether they fit the message. To address this gap, we propose Epigraphics, a web-based authoring system that treats an “epigraph” as the first-class object
Contact detection between curved fibres: high order makes a difference

, in the case of fibre systems, the impact of such choices on the retrieved contact forces. We highlight the presence of artifacts in the force response that are tightly related to the low-order geometry used for contact detection. Our analysis draws upon thorough comparisons between the high-order super-helix model and the low-order discrete elastic rod model. These reveal that when coupled to a low-order, segment-based detection scheme, both models yield spurious jumps in the contact force profile. Moreover, these artifacts are shown to be all the more visible as the geometry of fibres at contact is curved. In order to remove such artifacts we develop an accurate high-order detection scheme between two smooth curves, which relies on an efficient adaptive pruning strategy. We use this algorithm to detect contact between super-helices at high precision,
AN INTERACTIVE APPROACH TO IDENTIFY CRICKET SHOTS THROUGH DEEP LEARNING MECHANISM

In recent years, numerous sports have received significant attention and appeal. In the height of the current epidemic, the unavailability of any athletic events had left a significant number of people yearning to observe some game being played. Cricket is arguably the most popular sport in India, with millions of followers who watch the games obsessively. Fans are enthralled by the game and conduct in-depth analyses of the individual players and their abilities, particularly their shot choices. With the growth of fantasy leagues as well as other similar services, there is a growing interest in evaluating individuals who are playing well so that they may be selected for their teams. The manual procedure of batter shot recognition is amongst the most time-consuming and labor-intensive processes that might be automated. As a result, this study provides a successful cricket shot assessment approach that makes use of deep learning in the form of Convolutional Neural Networks to fulfill its objectives
Molecular Characterization of ORF esat Gene of M. tuberculosis Isolated from E.coli DE3

In this study, the open reading frame of a Secretory Antigenic Target (Esat) gene from M. tuberculosis in Escherichia coli BL21 (DE3) was cloned. Esat gene that was isolated from pulmonary TB patient was cloned into a plasmid vector (pGEM-Teasy) to construct pMB38. The E.coli DE3 clone carrying pMb38 was selected on X-gal medium. The expression of ORF in Esat gene was mediated using pRoExHTc under the control of Tre promoter and E.coli DE3 as host. Characterization of clones with recombinant plasmid was cut with restriction enzymes and sequencing of DNA inserts. Our results by BLAST analysis of sequencing showed 100% homology. It is hoped these proteins can then be used as an antigen for serological detection of latent TB in people.
An ecosystem-scale perspective of the net land methanol flux: synthesis of micrometeorological flux measurements

Methanol is the second most abundant volatile organic compound in the troposphere and plays a significant role in atmospheric chemistry. While there is consensus about the dominant role of living plants as the major source and the reaction with OH as the major sink of methanol, global methanol budgets diverge considerably in terms of source/sink estimates reflecting uncertainties in the approaches used to model, and the empirical data used to separately constrain these terms. Here we compiled micrometeorological methanol flux data from eight different study sites and reviewed the corresponding literature in order to provide a first cross-site synthesis of the terrestrial ecosystem-scale methanol exchange and present an independent data-driven view of the land–atmosphere methanol exchange. Our study shows that the controls of plant growth on the production, and thus the methanol emission magnitude, and stomatal conductance on the hourly methanol emission variability, established at the leaf level, hold across sites at the ecosystem-level. Unequivocal evidence for bi-directional methanol exchange at the ecosystem scale is presented. Deposition, which at some sites even exceeds methanol emissions, represents an emerging feature of ecosystem-scale measurements and is likely related to environmental factors favouring the formation of surface wetness. Methanol may adsorb to or dissolve in this surface water and eventually be chemically or biologically removed from it. Management activities in agriculture and forestry are shown to increase local methanol emission by orders of magnitude; they are however neglected at present in global budgets. While contemporary net land methanol budgets are overall consistent with the grand mean of the micrometeorological methanol flux measurements, we caution that the present approach of simulating methanol emission and deposition separately is prone to opposing systematic errors and does not allow taking full advantage of the rich information content of micrometeorological flux measurements.
Fourier rebinning and consistency equations for time-of-flight PET planograms

Due to the unique geometry, dual-panel PET scanners have many advantages in dedicated breast imaging and on-board imaging applications since the compact scanners can be combined with other imaging and treatment modalities. The major challenges of dual-panel PET imaging are the limited-angle problem and data truncation, which can cause artifacts due to incomplete data sampling. The time-of-flight (TOF) information can be a promising solution to reduce these artifacts. The TOF planogram is the native data format for dual-panel TOF PET scanners, and the non-TOF planogram is the 3D extension of linogram. The TOF planograms is five-dimensional while the objects are three-dimensional, and there are two degrees of redundancy. In this paper, we derive consistency equations and Fourier-based rebinning algorithms to provide a complete understanding of the rich structure of the fully 3D TOF planograms. We first derive two consistency equations and John’s equation for 3D TOF planograms. By taking the Fourier transforms, we obtain two Fourier consistency equations (FCEs) and the Fourier–John equation (FJE), which are the duals of the consistency equations and John’s equation, respectively. We then solve the FCEs and FJE using the method of characteristics. The two degrees of entangled redundancy of the 3D TOF data can be explicitly elicited and exploited by the solutions along the characteristic curves. As the special cases of the general solutions, we obtain Fourier rebinning and consistency equations (FORCEs), and thus we obtain a complete scheme to convert among different types of PET planograms: 3D TOF, 3D non-TOF, 2D TOF and 2D non-TOF planograms. The FORCEs can be used as Fourier-based rebinning algorithms for TOF-PET data reduction, inverse rebinnings for designing fast projectors, or consistency conditions for estimating missing data. As a byproduct, we show the two consistency equations are necessary and sufficient for 3D TOF planograms. Finally, we give numerical examples of implementation of a fast 2D TOF planogram projector and Fourier-based rebinning for a 2D TOF planograms using the FORCEs to show the efficacy of the Fourier-based solutions.
Primary donor photo-oxidation in photosystem I: a re-evaluation of (P700(+) - P700) Fourier transform infrared difference spectra.

Light-induced Fourier transform infrared (FTIR) difference spectroscopy has been used to study the photo-oxidation of the primary electron donor (P700) in PS I particles from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803. To aid in the interpretation of the spectra, PS I particles from a site-directed mutant of C. reinhardtii, in which the axial histidine ligand (HisA676) was changed to serine, were also studied. A high-frequency (3300-2600 cm(-1)) electronic transition is observed for all PS I particles, demonstrating that P700 is dimeric. The electronic band is, however, species-dependent, indicating some differences in the electronic structure of P700 and/or P700(+) in C. reinhardtii and Synechocystis sp. 6803. For PS I particles from C. reinhardtii, substitution of HisA676 with serine has little effect on the ester carbonyl modes of the chlorophylls of P700. However, the keto carbonyl modes are considerably altered. Comparison of (P700(+) - P700) FTIR difference spectra obtained using PS I particles from the wild type (WT) and the HS(A676) mutant of C. reinhardtii indicates that the mutation primarily exerts its influence on the P700 ground state. The 13(1) keto carbonyls of the chlorophylls of P700 of the wild type absorb at similar frequencies, which has previously made these transitions difficult to resolve. However, for the HS(A676) mutant, the 13(1) keto carbonyl of chlorophyll a or chlorophyll a' of P700 on PsaB or PsaA absorbs at 1703.4 or 1694.2 cm(-1), respectively, allowing their unambiguous resolution. Upon P700(+) formation, in both PS I particles from C. reinhardtii, the higher-frequency carbonyl band upshifts by approximately 14 cm(-1) while the lower frequency carbonyl downshifts by approximately 10 cm(-1). The similarity in the spectra for WT PS I particles from C. reinhardtii and Synechocystis sp. 6803 indicates that a similar interpretation is probably valid for PS I particles from both species. The mutant results allow for an interpretation of the behavior of the 13(1) keto carbonyls of P700 that is different from previous work [Breton, J., Nabedryk, E. , and Leibl, W. (1999) Biochemistry 38, 11585-11592], in which it was suggested that 13(1) keto carbonyls of P700 absorb at 1697 and 1639 cm(-1), and upshift by 21 cm(-1) upon cation formation. The interpretation of the spectra reported here is more in line with recent results from ENDOR spectroscopy and high-resolution crystallography.
Energy-aware routing in cluster-based sensor networks

There has been a growing interest in the applications of sensor networks. Since sensors are generally constrained in on-board energy supply, efficient management of the network is crucial in extending the life of the sensor. We present a novel approach for energy-aware and context-aware routing of sensor data. The approach calls for network clustering and assigns a less-energy-constrained gateway node that acts as a centralized network manager. Based on energy usage at every sensor node and changes in the mission and the environment, the gateway sets routes for sensor data, monitors latency throughout the cluster, and arbitrates medium access among sensors. Simulation results demonstrate that our approach can achieve substantial energy saving.
Tectonics of SE China: New insights from the Lushan massif (Jiangxi Province)

In south China the Lushan massif forms a topographic high of the South China Block south of the Qinling‐Dabie belt. The Lushan massif consists of two main lithotectonic units separated by a major tectonic contact: a Neoproterozoic (upper Sinian)‐Paleozoic unit comprising primarily unmetamorphosed sandstones overlies a Paleoproterozoic unit mainly composed of low‐pressure, high‐temperature gneisses and micaschists. Both units are cut by Cretaceous granitic intrusions. Three primary tectono‐metamorphic and magmatic events are recognized. The eastern part of the Lushan massif is cut by a NNE‐SSW trending ductile normal fault (D3 deformation) coeval to the emplacement of a 100–110 Ma leucogranite dated by 40Ar/39Ar laserprobe on biotite and muscovite. D2 deformation is responsible for the formation of a decakilometer‐scale NE‐SW trending upright anticline characterized by NE‐SW stretching and NW‐SE shortening. The age of this folding event is defined by a 127±1 (2σ) Ma U/Pb titanite date obtained for a syntectonic granodiorite and 40Ar/39Ar ages of 133 Ma for amphibole. This Cretaceous age also corresponds to the 40Ar/39Ar ages of 126 Ma found on syntectonic muscovites at the base of the Sinian unit. An older deformation event, D1, characterized by a top‐to‐the‐NW extensional decollement of the Sinian‐Paleozoic series above Proterozoic metamorphic rocks is related to the Triassic tectonics of the Dabieshan. Lastly, in the lower part of Sinian rocks, the occurrence of kyanite cataclased during D1 documents an older, poorly preserved, late Paleozoic‐early Mesozoic tectonometamorphic event (Dx) related to a blind thrust in the continental crust of the South China Block in the southern foreland of the Dabieshan.