Miracle-dz/UniFAR-FTUs huggingface dataset:
Light-Enhanced Cytotoxicity of Doxorubicin by Photoactivation

The combination of photodynamic therapy with chemotherapy (photochemotherapy, PCT) can lead to additive or synergistic antitumor effects. Usually, two different molecules, a photosensitizer (PS) and a chemotherapeutic drug are used in PCT. Doxorubicin is one of the most successful chemotherapy drugs. Despite its high efficacy, two factors limit its clinical use: severe side effects and the development of chemoresistance. Doxorubicin is a chromophore, able to absorb light in the visible range, making it a potential PS. Here, we exploited the intrinsic photosensitizing properties of doxorubicin to enhance its anticancer activity in leukemia, breast, and epidermoid carcinoma cells, upon irradiation. Light can selectively trigger the local generation of reactive oxygen species (ROS), following photophysical pathways. Doxorubicin showed a concentration-dependent ability to generate peroxides and singlet oxygen upon irradiation. The underlying mechanisms leading to the increase in its cytotoxic activity were intracellular ROS generation and the induction of necrotic cell death. The nuclear localization of doxorubicin represents an added value for its use as a PS. The use of doxorubicin in PCT, simultaneously acting as a chemotherapeutic agent and a PS, may allow (i) an increase in the anticancer effects of the drug, and (ii) a decrease in its dose, and thus, its dose-related adverse effects.
Asymptotic giant branch evolution of intermediate-mass stars as a function of mass and composition. II. Through the first major thermal pulse and the consequences of convective dredge-up

The aysmptotic giant branch evolution of intermediate-mass stars is further investigated following the end of the second dredge-up phase. The limiting mass for nondegenerate core carbon ignition is determined as a function of initial model mass and composition. A detailed description is given concerning the reignition of the hydrogen-burning shell through the appearance of the first major thermal pulse the luminosity of the heliun-burning shell is found to undergo a number of small oscillations which ultimately grow to become the first major pulse. The behavior of the first major thermal pulse is discussed in detail. The results of our calculations and those of other authors are combned for further elucidation of the core mass--interflash period relation and the core mass--luminosity relation. We present more information to support the idea that the latter relationship does depend on both the initial model mass and the initial Y abundance. Model lifetimes for models on the asymptotic giant branch are discussed both for the prethermal pulse phase and for the later thermal pulse phase.
Viscoelastic material behavior: models and discretization used in process simulator DIOS

It is a known fact that melted glass, such as SiO/sub 2/, shows viscoelastic behavior. But in the range of processing temperatures the mechanical properties of SiO/sub 2/ vary strongly. While below 800/spl deg/C the material behaves like an elastic solid, at temperatures above 1000/spl deg/C it shows nearly pure viscous properties. In this paper, the governing equation, the so-called constitutive equation, describing viscoelastic behavior, and its discretization are presented. The oxide viscosity depends on the local amount of shear stresses which leads to inhomogeneous material behavior and a nonlinear theory. This new mechanical model was implemented into the process simulator DIOS-ISE. Some obtained simulation results are shown and discussed.
Effect of Moisture and Oil Content in the Supercritical CO2 Defatting of Hermetia illucens Larvae

The supercritical defatting of H. illucens was scaled up at 450 bar and 60 °C from a 270 cm3 extraction cell to a vessel five times larger. Then, eight different H. illucens larvae batches, with variable content of oil (16.80–29.17% w/w) and moisture (4.45–15.95% w/w) were defatted. The effect of these parameters on yield and oil composition was analyzed. The presence of moisture in the larvae batch, in the range of the values studied, had no negative effect on the oil recovery efficiency, which was mainly determined by the initial content of oil in the larvae samples. Furthermore, no differences were determined in the fatty acid profile of the oils recovered, which were rich in saturated fatty acids, mainly lauric acid (ca. 50% w/w). Minor lipids, such as squalene and phytosterols, were determined in all the oil samples. The moisture content in the oils extracted was in the range of 0.118–1.706% w/w. Therefore, some samples exceeded the limits recommended for volatile matter in edible fats and oils (0.2%, including moisture). Yet, concerning the oil peroxide index, values were much lower than those corresponding to the oil extracted using hexane.
PRODUCTIVE PERFORMANCE OF THREE STRAINS OF PIGEONS (COLUMBA LIVIA DOMESTICA) UNDER EGYPTIAN CONDITIONS.

: This study was conducted to evaluate the growth performance of three strains of pigeons (the Local Egyptian pigeon, the Zagel pigeon, and the White Myrthes pigeon. A total number of 475 birds from the three strains were used to study live body weight at hatch, 1, 2, 3, and 4 weeks of age, and at sexual maturity, as well as growth rate between 0-1, 1-2, 2-3, 3-4, and 0-4 weeks of age. The results of body weight showed that Zagel and White Myrthes pigeons are superior to Local Egyptian pigeon in hatch weight and body weight at 7 days old then White Myrthes became the heaviest in later ages, while Local Egyptian pigeons was the lightest in all ages. Growth rate recorded the highest values in the first week of age (162-163 g) then decreased gradually to reach the minimum rate between 21 to 28 days old. White Myrthes has significantly faster growth during the whole period of study from hatch to 4 weeks of age (TGR). Estimates of heritability for body weights and growth rate showed moderate heritability expect for growth rate in the third week of age. The estimates ranged from 0.21 to 0.28 and from 0.09 to 0.28, for body weight and growth rate, respectively. Phenotypic correlations among body weight traits and growth rate traits were mostly high and positive which ranged between 0.63 to 0.82 and 0.12 to 0.77, respectively. Genetic correlation between the same traits were positive and ranged between 0.70 to 0.96 for body weight and 0.20 to 0.88 for growth rate. From the current results, White Myrthes strain was superior in most of the studied traits which give the possibility to involve it in future genetic programs for improving pigeon growth traits.
Harmonic maps and constant mean curvature surfaces in H 2 x R

We introduce a hyperbolic Gauss map into the Poincar´e disk for any surface in H2×R with regular vertical projection, and prove that if the surface has constant mean curvature H = 1/2, this hyperbolic Gauss map is harmonic. Conversely, we show that every nowhere conformal harmonic map from an open simply connected Riemann surface Σ into the Poincaré disk is the hyperbolic Gauss map of a two-parameter family of such surfaces. As an application we obtain that any holomorphic quadratic differential on Σ can be realized as the Abresch-Rosenberg holomorphic differential of some, and generically infinitely many, complete surfaces with H = 1/2 in H2 x R. A similar result applies to minimal surfaces in the Heisenberg group Nil3. Finally, we classify all complete minimal vertical graphs in H2 × R.
The Cannabis Plant as a Complex System: Interrelationships between Cannabinoid Compositions, Morphological, Physiological and Phenological Traits

Maintaining specific and reproducible cannabinoid compositions (type and quantity) is essential for the production of cannabis-based remedies that are therapeutically effective. The current study investigates factors that determine the plant’s cannabinoid profile and examines interrelationships between plant features (growth rate, phenology and biomass), inflorescence morphology (size, shape and distribution) and cannabinoid content. An examination of differences in cannabinoid profile within genotypes revealed that across the cultivation facility, cannabinoids’ qualitative traits (ratios between cannabinoid quantities) remain fairly stable, while quantitative traits (the absolute amount of Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabichromene (CBC), cannabigerol (CBG), Δ9-tetrahydrocannabivarin (THCV) and cannabidivarin (CBDV)) can significantly vary. The calculated broad-sense heritability values imply that cannabinoid composition will have a strong response to selection in comparison to the morphological and phenological traits of the plant and its inflorescences. Moreover, it is proposed that selection in favour of a vigorous growth rate, high-stature plants and wide inflorescences is expected to increase overall cannabinoid production. Finally, a range of physiological and phenological features was utilised for generating a successful model for the prediction of cannabinoid production. The holistic approach presented in the current study provides a better understanding of the interaction between the key features of the cannabis plant and facilitates the production of advanced plant-based medicinal substances.
Isospectral sets for boundary value problems on the unit interval

Abstract We analyse isospectral sets of potentials associated to a given ‘generalized periodic’ boundary condition in SL(2, R) for the Sturm-Liouville equation on the unit interval. This is done by first studying the larger manifold M of all pairs of boundary conditions and potentials with a given spectrum and characterizing the critical points of the map from M to the trace a + d Isospectral sets appear as slices of M whose geometry is determined by the critical point structure of the trace function. This paper completes the classification of isospectral sets for all real self-adjoint boundary conditions.
Discrete Element Method Simulations for Complex Granular Flows

This review article focuses on the modeling of complex granular flows employing the discrete element method (DEM) approach. The specific topic discussed is the application of DEM models for the study of the flow behavior of nonspherical, flexible, or cohesive particles, including particle breakage. The major sources of particle cohesion—liquid induced, electrostatics, van der Waals forces—and their implementation into DEM simulations are covered. These aspects of particle flow are of great importance in practical applications and hence are the significant foci of research at the forefront of current DEM modeling efforts. For example, DEM simulations of nonspherical grains can provide particle stress information needed to develop constitutive models for continuum-based simulations of large-scale industrial processes.
The Beginning of Everything: Finding the Seizure Onset

Ventral Hippocampal Formation Is the Primary Epileptogenic Zone in a Rat Model of Temporal Lobe Epilepsy Buckmaster PS, Reyes B, Kahn T, Wyeth M. J Neurosci. 2022;42(39):7482-7495. doi:10.1523/JNEUROSCI.0429-22.2022. PMID: 35995562, PMCID: PMC9525166. Temporal lobe epilepsy is common, but mechanisms of seizure initiation are unclear. We evaluated seizure initiation in female and male rats that had been systemically treated with pilocarpine, a widely used model of temporal lobe epilepsy. Local field potential (LFP) recordings from many brain regions revealed variable sites of earliest recorded seizure activity, but mostly the ventral hippocampal formation. To test whether inactivation of the ventral hippocampal formation would reduce seizures, mini-osmotic pumps were used to continually and focally deliver TTX. High doses of TTX infused unilaterally into the ventral hippocampal formation blocked seizures reversibly but also reduced LFP amplitudes in remote brain regions, indicating distant effects. A lower dose did not reduce LFP amplitudes in remote brain regions but did not reduce seizures when infused unilaterally. Instead, seizures tended to initiate in the contralateral ventral hippocampal formation. Bilateral infusion of the lower dose into the ventral hippocampal formation reduced seizure frequency 85%. Similar bilateral treatment in the amygdala was not effective. Bilateral infusion of the dorsal hippocampus reduced seizure frequency, but only 17%. Together, these findings reveal that the ventral hippocampal formation is a primary bilaterally independent epileptogenic zone, and the dorsal hippocampus is a secondary epileptogenic zone in pilocarpine-treated rats. This is consistent with many human patients, and the results further validate the LFP method for identifying seizure onset zones. Finally, the findings are more consistent with a focal mechanism of ictogenesis rather than one involving a network of interdependent nodes.
Convolutions of long integer sequences by means of number theoretic transforms over residue class polynomial rings

In a recent paper, Dubois and Venetsanopoulos [6] have derived methods for convolving sequences of numbers belonging to a ring S, using number theoretic transforms (NTT's) over an extension ring R of S. In this paper we obtain more explicit expressions for some of their results and, more important, improve the efficiency of their methods. Attention is focused on the case R = S[z]/(P(z)), that is, R is the quotient ring of S[z], modulo the principal ideal generated by a polynomial P(z) of degree n.
Optimization and Modeling of Citrobacter freundii AD119 Growth and 1,3-Propanediol Production Using Two-Step Statistical Experimental Design and Artificial Neural Networks

1,3-propanediol (1,3-PD) has a wide range of industrial applications. The most studied natural producers capable of fermenting glycerol to 1,3-PD belong to the genera Klebsiella, Citrobacter, and Clostridium. In this study, the optimization of medium composition for the biosynthesis of 1,3-PD by Citrobacter freundii AD119 was performed using the one-factor-at-a-time method (OFAT) and a two-step statistical experimental design. Eleven mineral components were tested for their impact on the process using the Plackett–Burman design. MgSO4 and CoCl2 were found to have the most pronounced effect. Consequently, a central composite design was used to optimize the concentration of these mineral components. Besides minerals, carbon and nitrogen sources were also optimized. Partial glycerol substitution with other carbon sources was found not to improve the bioconversion process. Moreover, although yeast extract was found to be the best nitrogen source, it was possible to replace it in part with (NH4)2SO4 without a negative impact on 1,3-PD production. As a part of the optimization procedure, an artificial neural network model of the growth of C. freundii and 1,3-PD production was developed as a predictive tool supporting the design and control of the bioprocess under study.
Simulated Trapping of Solar Energetic Protons for the 8–10 March 2012 Geomagnetic Storm: Impact on Inner Zone Protons as Measured by Van Allen Probes

Solar energetic protons (SEPs) have been shown to contribute significantly to the inner zone trapped proton population for energies <100 MeV and L > 1.3 (Selesnick et al., 2007, https://doi.org/10.1029/2006sw000275). The Relativistic Electron Proton Telescope (REPT) on the Van Allen Probes launched 30 August 2012 observed a double‐peaked (in L) inner zone population throughout the 7‐year lifetime of the mission. It has been proposed that a strong SEP event accompanied by a CME‐shock in early March 2012 provided the SEP source for the higher L trapped proton population, which then diffused radially inward to be observed by REPT at L ∼ 2. Here, we follow trajectories of SEP protons launched isotropically from a sphere at 7 Re in 15 s cadence fields from an Lyon‐Fedder‐Mobarry coupled to Rice Convection Model global magnetohydrodynamic (MHD) simulation driven by measured upstream solar wind parameters. The timescale of the interplanetary shock arrival is captured, launching a magnetosonic impulse propagating azimuthally along the dawn and dusk flanks inside the magnetosphere, shown previously to produce SEP trapping. The MHD‐test particle simulation uses Geostationary Operational Environmental Satellite (GOES) proton energy spectra to weight the initial radial profile required for the radial diffusion calculation over the following 2 years. GOES proton measurements also provide a dynamic outer boundary condition for radial diffusion. A direct comparison with REPT measurements 20 months following the trapping event in March 2012 supports this novel combination of short‐term and long‐term evolution of the newly trapped protons.
Preoperative Hilar and Mediastinal Lymph Node Staging in Patients with Suspected or Diagnosed Lung Cancer: Accuracy of 18F-FDG-PET/CT:A Retrospective Cohort Study of 138 Patients

The aim of this study was to evaluate the diagnostic accuracy of integrated 18F-fluorodeoxyglucose positron emission computed tomography (18F-FDG-PET/CT) in hilar and mediastinal lymph node (HMLN) staging of suspected or proven lung cancer, and to investigate potential risk factors for false negative and false positive HMLN metastases. We retrospectively analyzed 162 consecutive patients with suspected or pathologically proven non-small cell lung cancer (NSCLC). The receiver operating characteristic (ROC) curve was generated to determine the diagnostic efficacy of 18F-FDG-PET/CT. Univariate and multivariate analyses were conducted to detect risk factors of false positives and false negatives. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of integrated 18F-FDG-PET/CT in detecting HMLN metastases were 59.1% (26/44), 69.1% (65/94), 47.3% (26/55), 78.3% (65/83), and 65.9% (91/138), respectively. The ROC curve showed an area under the curve (AUC) of 0.625 (95%-CI 0.468–0.782). The incidence of false negative and false positive HMLN metastases was 21.7% (18/83) and 52.7% (29/55), respectively. Our data shows that integrated 18F-FDG-PET/CT staging provides lower specificity and sensitivity. This confirms the ESTS guideline on lymph node staging for PET-positive HMLN. Yet it advocates more invasive staging even for PET-negative HMLN.
Fibroblast-Generated Extracellular Matrix Guides Anastomosis during Wound Healing in an Engineered Lymphatic Skin Flap

A healthy lymphatic system is required to return excess interstitial fluid back to the venous circulation. However, up to 49% of breast cancer survivors eventually develop breast cancer-related lymphedema due to lymphatic injuries from lymph node dissections or biopsies performed to treat cancer. While early-stage lymphedema can be ameliorated by manual lymph drainage, no cure exists for late-stage lymphedema when lymph vessels become completely dysfunctional. A viable late-stage treatment is the autotransplantation of functional lymphatic vessels. Here we report on a novel engineered lymphatic flap that may eventually replace the skin flaps used in vascularized lymph vessel transfers. The engineered flap mimics the lymphatic and dermal compartments of the skin by guiding multi-layered tissue organization of mesenchymal stem cells and lymphatic endothelial cells with an aligned decellularized fibroblast matrix. The construct was tested in a novel bilayered wound healing model and implanted into athymic nude rats. The in vitro model demonstrated capillary invasion into the wound gaps and deposition of extracellular matrix fibers, which may guide anastomosis and vascular integration of the graft during wound healing. The construct successfully anastomosed in vivo, forming chimeric vessels of human and rat cells. Overall, our flap replacement has high potential for treating lymphedema.
Biomarkers of Aggressive Prostate Cancer at Diagnosis

In the United States, prostate cancer (CaP) remains the second leading cause of cancer deaths in men. CaP is predominantly indolent at diagnosis, with a small fraction (25–30%) representing an aggressive subtype (Gleason score 7–10) that is prone to metastatic progression. This fact, coupled with the criticism surrounding the role of prostate specific antigen in prostate cancer screening, demonstrates the current need for a biomarker(s) that can identify clinically significant CaP and avoid unnecessary biopsy procedures and psychological implications of being diagnosed with low-risk prostate cancer. Although several diagnostic biomarkers are available to clinicians, very few comparative trials have been performed to assess the clinical effectiveness of these biomarkers. It is of note, however, that a majority of these clinical trials have been over-represented by men of Caucasian origin, despite the fact that African American men have a 1.7 times higher incidence and 2.1 times higher rate of mortality from prostate cancer. Biomarkers for CaP diagnosis based on the tissue of origin include urine-based gene expression assays (PCA3, Select MDx, ExoDx Prostate IntelliScore, Mi-Prostate Score, PCA3-PCGEM1 gene panel), blood-based protein biomarkers (4K, PHI), and tissue-based DNA biomarker (Confirm MDx). Another potential direction that has emerged to aid in the CaP diagnosis include multi-parametric magnetic resonance imaging (mpMRI) and bi-parametric magnetic resonance imaging (bpMRI), which in conjunction with clinically validated biomarkers may provide a better approach to predict clinically significant CaP at diagnosis. In this review, we discuss some of the adjunctive biomarker tests along with newer imaging modalities that are currently available to help clinicians decide which patients are at risk of having high-grade CaP on prostate biopsy with the emphasis on clinical utility of the tests across African American (AA) and Caucasian (CA) men.
Inviscid waves on a Lamb–Oseen vortex in a rotating stratified fluid: consequences for the elliptic instability

The inviscid waves propagating on a Lamb–Oseen vortex in a rotating medium for an unstratified fluid and for a strongly stratified fluid are analysed using numerical and asymptotic approaches. By a local Lagrangian description, we first provide the characteristics of the local plane waves (inertia–gravity waves) as well as the local growth rate associated with the centrifugal instability when the vortex is unstable. A global WKBJ approach is then used to determine the frequencies of neutral core modes and neutral ring modes. We show that these global Kelvin modes only exist in restricted domains of the parameters. The consequences of these domain limitations for the occurrence of the elliptic instability are discussed. We argue that in an unstratified fluid the elliptic instability should be active in a small range of the Coriolis parameter which could not have been predicted from a local approach. The wavenumbers of the sinuous modes of the elliptic instability are provided as a function of the Coriolis parameter for both an unstratified fluid and a strongly stratified fluid.
The effect of adiabatic focusing upon charged particle propagation in random magnetic fields

The charged particles considered in this paper are scattered by random fields while they propagate along the diverging lines of force of a spatially inhomogeneous guiding field. Their longitudinal transport is described in terms of the eigenfunctions of a Sturm-Liouville operator which incorporates the effect of adiabatic focusing along with that of scattering. The relaxation times and characteristic velocities, which appear in this matrix formulation of the transport problem are graphed and tabulated. Explicit formulae which describe the particle density profile that results from a localized impulsive injection are derived for two different regimes. In the first regimes, where focusing is relatively weak, a diffusive mode of propagation is dominant, but coherent modes are also present, and they become prominent as the intensity of focusing increases. In the second regime, where focusing is strong and where diffusion does not occur, the propagation is purely coherent. The existence of this supercoherent mode of particle transport opens up many possibilities are explored in an accompanying paper. (AIP)