Miracle-dz/UniFAR-FTUs huggingface dataset:
Tree seedling reponses to wastewater irrigation on a reforested old field in southern Michigan.

In recent years, the number of'municipal wastewater irrigation projects utilizing forest ecosystems for nutrient recycling and groundwater recharge has continued to grow. Land managers involved with these projects have, in the same time period, increased their demand for scientific data which better defines the capabilities and limitations of various forest species-site combinations. Smith and Evans (1977) suggested that an initial step in planning for wastewater recycling in forests was to assess the species ecological requirements in terms of water and nutrients. Identifying forest species adapted to the moisture and nutrient regimen.provided by wastewater irrigation upon a specific type of forest site is a prerequisite to sound irrigation project management. Old fields have come under the increased scrutiny of land managers as potential forest sites where plantations established near municipalities could benefit from wastewater irrigation. Old fields occupy millions of hectares in the eastern United States and are typically the target of reforestation efforts by private and public land managers. These sites have a history of agricultural management which has significantly modified their native vegetation and soil characteristics. Old fields contain a well developed Ap soil horizon supporting a diverse array.of native and exotic herbaceous and woody plant species. Intense competition from these species for available water and nutrients is a major obstacle to tree plantation establishment and development. Cultural treatments such as wastewater irrigation, which supplement site water and nutrient resources, may enhance seedling survival and growth if weed competition is controlled. Weed control is particularly critical for hardwood
Enhancing Nutritional Profile of Pasta: The Impact of Sprouted Pseudocereals and Cushuro on Digestibility and Health Potential

We hypothesized that optimizing the formulation of pasta by incorporating sprouted pseudocereal flours, specifically quinoa (Chenopodium quinoa Willd) or kiwicha (Amaranthus caudatus L.) and cushuro (Nostoc sphaericum Vaucher ex Bornet & Flahault) flours, could offer the potential to simultaneously enhance nutritional quality and health-promoting properties in pasta. In this study, our objective was to optimize the formulation of composite flour (a ternary blend of wheat, sprouted pseudocereal, and cushuro flours) using a mixture composite design to maximize total soluble phenolic compounds (TSPC), γ-aminobutyric acid (GABA), antioxidant activity, and mineral bioaccesilability by reducing phytic acid (PA) content. Two optimal formulations were identified: one consisting of 79% wheat flour (WF), 13% SQF, and 8% CuF (oPQC), and the other composed of 70% WF, 15% SKF, and 15% CuF (oPKC). These optimized pastas exhibited reduced starch content and notably higher levels of total dietary fiber (1.5–3.61-fold), protein (1.16-fold), fat (1.3–1.5-fold), ash (2.2–2.7-fold), minerals (K, Na, Fe, Zn, Mg, Mn, and Ca), PA (3–4.5-fold), TSPC (1.3–1.9-fold), GABA (1.2–2.6-fold), and ORAC (6.5–8.7-fold) compared to control pasta (100% WF). Notably, the glycemic index of oPQC (59.8) was lower than that of oPKC (54.7) and control pasta (63.1). The nutritional profile of the optimized pasta was largely retained after cooking, although some significant losses were observed for soluble dietary fiber (18.2–44.0%), K (47.5–50.7%), Na (42.5–63.6), GABA (41.68–51.4%), TSPC (8–18%), and antioxidant activity (45.4–46.4%). In vitro digestion of cooked oPQC and oPKC demonstrated higher bioaccessible content of GABA (6.7–16.26 mg/100 g), TSPC (257.7–261.8 mg GAE/100 g), Ca (58.40–93.5 mg/100 g), and Fe (7.35–7.52 mg/100 g), as well as antioxidant activity (164.9–171.1 µmol TE/g) in intestinal digestates compared to control pasta. These findings suggest that the incorporation of sprouted pseudocereals and cushuro flour offers a promising approach to enhance the nutritional quality and bioactive content of wheat-based pasta, potentially providing health benefits beyond traditional formulations.
Cultural Conceptualisations in English Words: A Study of Aboriginal Children in Perth

This study explored conceptualisations that two groups of Aboriginal and Anglo- Australian students attending metropolitan schools in Western Australia instantiate through the use of English words. At the time of the study, many educators believed that both these groups of students spoke the same dialect. A group of 30Aboriginal primary school students and a matching group of Anglo-Australian students participated in the study. Thirty-two English words were used as prompts to evoke schemas and categories in participants. The responses were then interpreted using an ethnographic approach toward the identification of cultural conceptualisations. These responses were compared within and between the two groups. The analysis of the data provided evidence for the operation of two distinct, but overlapping, conceptual systems among the two cultural groups studied. The discrepancies between the two systems largely appear to be rooted in the cultural systems that characterise each group while the overlap between the two conceptual systems appears to arise from several phenomena such as experience in similar physical environments. One of the implications of the findings is that a critical defining feature of some varieties of a language may be their conceptual basis, rather than so much their grammatical and/or phonological features. This observation calls for further exploration and perhaps a revisiting of the notion of ‘dialect’.
Heterozygous Knockout of ARID4B Using CRISPR/Cas9 Attenuates Some Aggressive Phenotypes in a Breast Cancer Cell Line

Breast cancer is one of the leading causes of death in women around the world. Over time, many genes and mutations that are associated with the development of this disease have been identified. However, the specific role of many genes has not yet been fully elucidated. Higher ARID4B expression has been identified as a risk factor for diverse cancer types. Silencing experiments also showed that ARID4B is associated with developing cancer-associated characteristics. However, no transcriptomic studies have shown the overall cellular effect of loss of function in breast cancer in humans. This study addresses the impact of loss-of-function mutations in breast cancer MCF-7 cells. Using the CRISPR/Cas9 system, we generated mutations that caused heterozygous truncated proteins, isolating three monoclonal lines carrying insertions and deletions in ARID4B. We observed reduced proliferation and migration in in vitro experiments. In addition, from RNA-seq assays, a differential expression analysis shows known and novel deregulated cancer-associate pathways in mutated cells supporting the impact of ARID4B. For example, we found the AKT-PI3K pathway to be altered at the transcript level but through different genes than those reported for ARID4B. Our transcriptomic results also suggest new insights into the role of ARID4B in aggressiveness by the epithelial-to-mesenchymal transition and TGF-β pathways and in metabolism through cholesterol and mevalonate pathways. We also performed exome sequencing to show that no off-target effects were apparent. In conclusion, the ARID4B gene is associated with some aggressive phenotypes in breast cancer cells.
Quantitative and Biogeographic Analysis of the Flora of the Sultanate of Oman

The number of vascular plants recorded so far is 1204. Species of gymnosperms and vascular cryptogams is low (3 spp. and 19 spp. respectively). Families Poaceae (201 spp.), Asteraceae (98 spp.), Fabaceae (81 spp.) and Euphorbiaceae (39 spp.) are amongst those which have the highest number of species. There are ninety-three endemic species (7.7%), of which fifty-seven (4.7%) occur in southern Oman. Biogeographically, the southwest region of Oman falls in the Somalia-Masai regional centre of endemism, the central part in the Saharan regional subzone, the western part in the Saharo-Arabian regional subzone and the northern part in the NuboSindian local centre of endemism.
Navigating the Challenges of Gluten Enteropathy and Infertility: The Role of Celiac-Related Antibodies and Dietary Changes

Celiac disease (CD) is an autoimmune condition that is initiated in genetically susceptible individuals by the exposure of the intestines to gluten, and the early start of symptoms is related to malabsorption. Atypical variants of the illness are often identified in adulthood and are frequently associated with manifestations outside of the intestines, including metabolic osteopathy, anemia, and dermatitis herpetiformis. But also, empirical data suggest a correlation between CD and reproductive abnormalities, including repeated abortions. Infertility and repeated miscarriages frequently manifest in women diagnosed with CD and may serve as the initial clinical indication of a subclinical form. Furthermore, the condition may manifest as amenorrhea, infertility, and the delivery of infants with a low birth weight. Regarding the mechanisms of CD in infertility, along with the anti-tTG action to hinder the invasiveness of trophoblast, these antibodies could damage endometrial angiogenesis, which has been shown in in vitro models with human endometrial cells and in vivo in murine models. Another important aspect is the role of nutrient deficiencies, such as zinc deficiency (connected to impaired hormone production, secondary amenorrhea, and pre-eclampsia) and folic acid, etc. Therefore, our objective was to conduct a comprehensive review of the existing literature pertaining to this specific topic and to elucidate the role of the autoantibodies in its pathogenesis.
Erosion onset of a cohesionless granular medium by an immersed impinging round jet

Among diﬀerent devices developed quite recently to quantify the resistance to erosion of a natural soil within the broader context of dyke safety, the most commonly used is probably the Jet Erosion Test (JET) in which a scouring crater is induced by impingement of an immersed water jet. A comprehensive experimental investigation on the jet erosion in the speciﬁc situation of a cohesionless granular material is presented here. The tests were performed combining special optical techniques allowing for an accurate measurement of the scouring onset and evolution inside an artiﬁcially translucent granular sample. The impinging jet hydrodynamics are also analyzed empirically validating the use of a self-similar theoretical framework for the laminar round jet. The critical conditions at the onset of erosion appear to be best described by a dimensionless Shields number based on the inertial drag force created by the ﬂuid ﬂow on the eroded particles rather than on the pressure gradients around them. To conclude, a tentative empirical model for the maximal ﬂow velocity initiating erosion at the bottom of the scoured crater is put forward and discussed in the light of some preliminary results.
Non‑persistence to Oral Anticoagulation Therapy in Elderly Patients with Non‑valvular Atrial Fibrillation

Purpose To investigate the reasons for elderly atrial fibrillation (AF) patients not continuing their oral anticoagulation (OAC) treatment and the factors that influence this behavior. Methods Elderly AF patients (aged≥75 years) hospitalized from December 2019 to May 2022 were consecutively enrolled. Clinical, demographic, and concomitant medication data were collected. The endpoint was defined as OAC discontinuation for more than 30 days or a switch to an alternative therapy. Predictors of OAC non-persistence were investigated using a multivariable Cox regression model. Results This study included 560 participants (51.1% men, mean age 80.9±0.2 years). During a median follow-up of 20 months, medication persistence was observed in 322 patients (57.5%). Non-persistence was found to be significantly higher with warfarin than with NOAC (48.8% vs 33.6%, p = 0.006). In the multivariate analysis, OAC non-persistence was independently predicted by a history of permanent pacemaker implantation, the use of antiplatelet drugs, employee Medicare, living with children, college degree or above, and persistent AF (HR = 1.580, 1.586, 0.604, 0.668, 0.028, 0.769, p < 0.05, respectively). Treatment discontinuation within 3 months of discharge was observed in a large number of patients (81.8%). Medication discontinuation due to bleeding was more frequently observed in patients who continued for longer than 3 months (p < 0.001), while discontinuation due to patient preference was more frequent in those with shorter durations (≤3 months) (p = 0.049). Patient preference was the second leading cause of non-persistence in patients, regardless of whether they were taking warfarin or NOAC. Conclusion OAC non-persistence remains high among elderly AF patients during long-term follow-up, with a significant proportion discontinuing shortly after discharge. This pattern of non-persistence is heavily influenced by demographic factors and patient preference. Further interventions should be developed based on the reasons and risk factors to improve persistence and initiated early in the treatment process.
Hong Kong–Macau Severe Hives and Angioedema Referral Pathway

Background Urticaria (defined as the presence of hives, angioedema, or both) can be caused by a variety of etiologies ranging from more common conditions such as chronic spontaneous urticaria (CSU) to rarer conditions such as hereditary angioedema (HAE). Specialist referral may be necessary in cases of severe urticaria or HAE, but access to specialist services remains limited in certain regions, such as the Greater Bay Area (GBA) of China. To address this, the Hong Kong–Macau Severe Hives and Angioedema Referral Pathway (SHARP) was initiated by the Hong Kong Institute of Allergy and Macau Society of Dermatology to promote multidisciplinary collaboration and regional exchange of expertise in the diagnosis and management of severe urticaria. Methods A nominated task force of dermatologists and immunologists who manage patients with severe urticaria formulated the consensus statements (CS) using the Delphi method. The consensus was defined a priori as an agreement of ≥80%. Results A total of 24 CS were formulated, including four statements on classifications and definitions, seven statements on diagnosis, and 13 statements on management and referral. The definitions for acute/chronic urticaria and severe CSU were stated. Unnecessary investigations and inappropriate medications were discouraged. The characteristics and recommended approach to suspected bradykinergic angioedema were specified. Stepwise treatment options using second-generation antihistamines, omalizumab, or cyclosporin for patients with CSU were addressed, and the importance of access to HAE-specific medications was emphasized. Furthermore, an integrated referral pathway for patients with severe hives and angioedema was constructed. Conclusion The SHARP provides guidance for the management and specialist referral of patients with severe hives and angioedema in Hong Kong and Macau.
Paleozoic Decollement Displaced the Surface Trace of Iapetus Ocean Closure in Northern Appalachians

The northern Appalachians record accretion of Laurentia‐ and Gondwana‐derived terranes to Laurentian margin during the formation of Pangea. Terrane bounding structures, key to reconstructing this history, are poorly understood at depth. We use teleseismic receiver functions to probe the crust beneath Appalachian terranes in Québec and Maine. We identify an eastward‐dipping intra‐crustal boundary extending beneath the trace of the Iapetus Ocean closure that separates the peri‐Laurentian Dunnage and the peri‐Gondwanan Gander terranes. Systematic fabric in rocks is a likely cause of anisotropic seismic properties associated with it. Depth and position associate this boundary with a previously identified décollement separating authochtonous Laurentian basement from rocks emplaced during Pangea's assembly. Neither the décollement nor the crust‐mantle boundary under our profile change beneath the surface trace of the Iapetus Ocean closure, suggesting that it has been transported to the northwest relative to the actual boundary between mantle lithospheres of former Laurentia and Gondwana.
The Effect of Student–Teacher Relationships on Students’ Math Motivation Across EU Countries

Using PISA 2012 data, we conducted multilevel modeling analyses to explore how student–teacher relationships (STRs) affected mathematics motivation across EU countries. We compared this relationship across high and low achievers and explored how sex, economic, social, and cultural status (ESCS), ability grouping, class size, and teacher intentions moderated this relationship. Results show that positive STR was associated with higher motivation. High-ability students, boys, and high ESCS students had higher motivation, but we found no statistically significant interactions between STR and low or high ability. Class size, ability grouping, and teacher intentions had no statistically significant effects on mathematics motivation. Overall, our findings suggest that positive perceptions of STR can positively impact student motivation in mathematics and that this effect is consistent across different demographic groups. Thus, general trends around STR and motivation also apply to high-achieving populations.
Antimicrobial and quorum sensing inhibitory activity of epiphytic bacteria isolated from the red alga Halymenia durvillei

Halymenia durvillei is a red alga that is commonly utilized in the Philippines as food and as a source of high-value natural products for industrial applications. However, there are no studies regarding the microbial community associated with H. durvillei and its potential applications. This study aimed to isolate and identify the epiphytic bacteria of H. durvillei and determine their antimicrobial and quorum sensing inhibitory (QSI) effects. The thalli of H. durvillei were collected at the shores of Santa Fe, Bantayan, Cebu, Philippines. Bacterial isolates were identified using 16S rRNA, and their ethyl acetate (EtOAc) extracts were subjected to antimicrobial susceptibility tests against representative species of yeast and Gram-negative and Gram-positive bacteria. Their QSI activity against Chromobacterium violaceum was also determined. Fourteen distinct bacterial colonies belonging to four genera, namely Alteromonas (3), Bacillus (5), Oceanobacillus (1) and Vibrio (5), were successfully isolated and identified. All 14 bacterial isolates exhibited antibacterial effects. EPB9, identified as Bacillus safensis , consistently showed the strongest inhibition against Escherichia coli , Staphylococcus aureus and Staphylococcus epidermidis , with minimum inhibitory concentrations (MICs) ranging from 0.0625 to 1.0 mg ml−1. In contrast, all 14 isolates showed weak antifungal effects. Both B. safensis (EPB9) and Bacillus australimaris (EPB15) exhibited QSI effects at 100 mg ml−1, showing opaque zones of 3.1±0.9 and 3.8±0.4 mm, respectively. This study is the first to isolate and identify the distinct microbial epiphytic bacterial community of H. durvillei and its potential as an abundant resource for new antibacterial and QSI bioactives.
First Inventory of the Semi-Submerged Marine Caves in South Dinarides Karst (Adriatic Coast) and Preliminary List of Species

1 Institute for Marine Biology, Dobrota b.b. 85330 Kotor, Montenegro. 2 Archipelagos environment and development (NGO), GR-281 00 Lourdata, Kefalonia, Greece. 3 Archipelagos-ambiente e sviluppo, Italia Calle Asiago 4 (Sant'Elena) (NGO), 30132 Venezia, Italy. 4 Mediterranean Center for Environmental Monitoring (NGO), I. Milutinovića 7, Sutomore, Montenegro. 5 Biospeleological Society of Montenegro (NGO), Zabjelo b.b. , Podgorica, Montenegro.
Impact of Poor Sleep Quality on the Academic Performance of Medical Students

Introduction: Adequate sleep has a crucial role in enhancing cognitive skills especially memory retention. Poor night time sleep quality and the consequent daytime sleepiness affect physical and cognitive health of students and their academic performance. The aim of this study is to find whether or not poor academic performance is a consequence of poor sleep quality among Pakistani medical students. Methods: It was an observational, cross-sectional study conducted with undergraduate medical students. Pittsburgh Sleep Quality Index (PSQI) was used to assess the sleep quality. Academic performance was assessed by mean grade point average (GPA) of the students. The data were analyzed using the Statistical Package for the Social Sciences (SPSS) 22.0 (IBM Corp., Armonk, NY, USA). Results: There were 512 (64.24%) students with global PSQI score ≥5 indicating poor sleep quality. The mean GPA of poor sleepers was 2.92 ± 1.09 which was significantly lower than that of good sleepers (p < 0.0001). In the group of students who scored lower GPA (2.0-2.7), 28.2% had very bad subjective sleep quality, 29.05% had sleep latency of 16-30 min, 29.4% had sleep duration of <5-7 h, 27.8% had sleep efficiency of <85%, and 37.7% experienced daytime dysfunction almost every day. Conclusion: Medical students of Pakistan have poor sleep quality which has a negative impact on their academic performance. Adequate sleep is essential to refresh the students every day and help them in learning and memory processing. Medical students and their facilitators should comprehend the negative effects of sleep deprivation on student academics and should take adequate measures to improve the sleep quality of students.
Soil bioremediation approaches for petroleum hydrocarbon polluted environments

Increasing industrialisation, continued population growth and heavy demand and reliance on petrochemical products have led to unprecedented economic growth and development. However, inevitably this dependence on fossil fuels has resulted in serious environmental issues over recent decades. The eco-toxicity and the potential health implications that petroleum hydrocarbons pose for both environmental and human health have led to increased interest in developing environmental biotechnology-based methodologies to detoxify environments impacted by petrogenic compounds. Different approaches have been applied for remediating polluted sites with petroleum derivatives. Bioremediation represents an environmentally sustainable and economical emerging technology for maximizing the metabolism of organic pollutants and minimizing the ecological effects of oil spills. Bioremediation relies on microbial metabolic activities in the presence of optimal ecological factors and necessary nutrients to transform organic pollutants such as petrogenic hydrocarbons. Although, biodegradation often takes longer than traditional remediation methods, the complete degradation of the contaminant is often accomplished. Hydrocarbon biodegradation in soil is determined by a number of environmental and biological factors varying from site to site such as the pH of the soil, temperature, oxygen availability and nutrient content, the growth and survival of hydrocarbon-degrading microbes and bioavailability of pollutants to microbial attack. In this review we have attempted to broaden the perspectives of scientists working in bioremediation. We focus on the most common bioremediation technologies currently used for soil remediation and the mechanisms underlying the degradation of petrogenic hydrocarbons by microorganisms.