Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of different total Leishmania amazonensis antigens for the development of a first-generation vaccine formulated with a Toll-like receptor-3 agonist to prevent cutaneous leishmaniasis

BACKGROUND Unfortunately, no any vaccine against leishmaniasis has been developed for human use. Therefore, a vaccine based on total Leishmania antigens could be a good and economic approach; and there are different methodologies to obtain these antigens. However, it is unknown whether the method to obtain the antigens affects the integrity and immune response caused by them. OBJECTIVES to compare the protein profile and immune response generated by total L. amazonensis antigens (TLA) produced by different methods, as well as to analyse the immune response and protection by a first-generation vaccine formulated with sonicated TLA (sTLA) and polyinosinic:polycytidylic acid [Poly (I:C)]. METHODS TLA were obtained by four different methodologies and their integrity and immune response were evaluated. Finally, sTLA was formulated with Poly (I:C) and their protective immune response was measured. FINDINGS sTLA presented a conserved protein profile and induced a strong immune response. In addition, Poly (I:C) improved the immune response generated by sTLA. Finally, sTLA + Poly (I:C) formulation provided partial protection against L. amazonensis infection. MAIN CONCLUSIONS The protein profile and immune response depend on the methodology used to obtain the antigens. Also, the formulation sTLA + Poly (I:C) provides partial protection against cutaneous leishmaniasis in mice.
Incorporation of a dried maggots' meal in growing rats diets: pathological risks?

Three groups of ten rats in growth fed with three diets differing only in the amount of dried maggots meal (DMM): 2.5%, 5% and 7.5%: DMM 2.5 , DMM 5 and DMM 7.5 . Assay of plasma biochemical parameters of all the rats did not detect diseases or physiological abnormalities. However, biometrics kidneys and livers of rats showed changes. The kidneys of rats decreased weight of 4.00%, 1.30% and 2.70% respectively in diets DMM 2.5 , DMM 5 and DMM 7.5 . As for the liver, their weight increased by 1.10%, 3.00% and 12.40% respectively with diets DMM 2.5 , DMM 5 and DMM 7.5 . These observations may reflect histopathological abnormalities of the kidneys and livers of rats that are to detect.
Regulation of vocal precision by noradrenergic modulation of a motor nucleus.

Recent theories of norepinephrine (NE) function suggest that NE modulates the transition between stereotyped, goal-directed behavior and more variable, exploratory behaviors that facilitate learning and adaptation. We provide evidence for context-dependent switching by NE that is analogous to this explore/exploit strategy in the vocal system of the zebra finch (Taeniopygia guttata). Stimulation of the locus coeruleus, the major source of NE in the brain, decreases song trial-to-trial variability, transforming the variable, exploratory "undirected" song into song that resembles the more stereotyped, exploitative "directed" song that males sing to females. This behavioral switch is mediated by NE acting directly on a cortical motor nucleus that integrates inputs from a premotor cortical nucleus and a basal ganglia circuit necessary for vocal motor learning. These findings suggest that NE can act directly on the motor system to influence the transition between exploratory and exploitative behavioral strategies.
The Landscape of Tumor-Specific Antigens in Colorectal Cancer

Over the last few decades, major efforts in cancer research and treatment have intensified. Apart from standard chemotherapy approaches, immunotherapy has gained substantial traction. Personalized immunotherapy has become an important tool for cancer therapy with the discovery of immune checkpoint inhibitors. Traditionally, tumor-associated antigens are used in immunotherapy-based treatments. Nevertheless, these antigens lack specificity and may have increased toxicity. With the advent of next-generation technologies, the identification of new tumor-specific antigens is becoming more important. In colorectal cancer, several tumor-specific antigens were identified and functionally validated. Multiple clinical trials from vaccine-based and adoptive cell therapy utilizing tumor-specific antigens have commenced. Herein, we will summarize the current landscape of tumor-specific antigens particularly in colorectal cancer.
Aspects of reproductive biology of Labeo horie Heckel (Pisces: Cyprinidae) in Lake Chamo, Ethiopia

Labeo horie Heckel is increasingly becoming commercially important in Lake Chamo but its reproductive biology in the lake is not well enough to guide its management. Sex ratio, breeding season, length at first maturity and fecundity of L. horie were studied from 1197 fish samples collected between August 1998 and October 1999 in Lake Chamo, Ethiopia. The sex ratio was significantly different (χ 2 = 12.12, P < 0.001). The peak-breeding period was during the rainy months of March to May, during which time more than 90% of both sexes had ripe gonads. The size at maturity ( Lm50 ) of males was 52 cm while the Lm50 of females was 62 cm. The smallest ripe male in the catch was 46.7 cm and weighed 890 g while the smallest ripe female caught was 49.5 cm and weighed 1145 g. The weight of ripe ovaries ranged from 54.3 g to 991.8 g and contained between 68,400 and 1,320,400 eggs. Relative fecundity ranged between 60 and 290 eggs per g of body weight. The relationships between fecundity and total length and between fecundity and total weight were curvilinear, while the relationship between fecundity and ovary weight was linear. L. horie conformed to the general pattern of reproduction in a tropical environment where peak-breeding activity occurred during the rainy season. In order to protect the spawning population, fishing pressure should be minimized during breeding time at the shallower littoral regions.
Molecular and Imaging Biomarkers in Alzheimer’s Disease: A Focus on Recent Insights

Alzheimer’s disease (AD) is the most common neurodegenerative disease among the elderly, affecting millions of people worldwide and clinically characterized by a progressive and irreversible cognitive decline. The rapid increase in the incidence of AD highlights the need for an easy, efficient and accurate diagnosis of the disease in its initial stages in order to halt or delay the progression. The currently used diagnostic methods rely on measures of amyloid-β (Aβ), phosphorylated (p-tau) and total tau (t-tau) protein levels in the cerebrospinal fluid (CSF) aided by advanced neuroimaging techniques like positron emission tomography (PET) and magnetic resonance imaging (MRI). However, the invasiveness of these procedures and the high cost restrict their utilization. Hence, biomarkers from biological fluids obtained using non-invasive methods and novel neuroimaging approaches provide an attractive alternative for the early diagnosis of AD. Such biomarkers may also be helpful for better understanding of the molecular mechanisms underlying the disease, allowing differential diagnosis or at least prolonging the pre-symptomatic stage in patients suffering from AD. Herein, we discuss the advantages and limits of the conventional biomarkers as well as recent promising candidates from alternative body fluids and new imaging techniques.
Nutritional Supplements to Support Resistance Exercise in Countering the Sarcopenia of Aging

Skeletal muscle plays an indispensable role in metabolic health and physical function. A decrease in muscle mass and function with advancing age exacerbates the likelihood of mobility impairments, disease development, and early mortality. Therefore, the development of non-pharmacological interventions to counteract sarcopenia warrant significant attention. Currently, resistance training provides the most effective, low cost means by which to prevent sarcopenia progression and improve multiple aspects of overall health. Importantly, the impact of resistance training on skeletal muscle mass may be augmented by specific dietary components (i.e., protein), feeding strategies (i.e., timing, per-meal doses of specific macronutrients) and nutritional supplements (e.g., creatine, vitamin-D, omega-3 polyunsaturated fatty acids etc.). The purpose of this review is to provide an up-to-date, evidence-based account of nutritional strategies to enhance resistance training-induced adaptations in an attempt to combat age-related muscle mass loss. In addition, we provide insight on how to incorporate the aforementioned nutritional strategies that may support the growth or maintenance of skeletal muscle and subsequently extend the healthspan of older individuals.
Anti-Inflammatory and Barrier-Stabilising Effects of Myrrh, Coffee Charcoal and Chamomile Flower Extract in a Co-Culture Cell Model of the Intestinal Mucosa

Recent clinical evidence suggests the efficacy of a traditional herbal medicinal product containing myrrh (Commiphora molmol Engl.), coffee charcoal (Coffea arabica L.) and chamomile flower dry extract (Matricaria chamomilla L.) in the therapy of inflammatory bowel diseases (IBD). However, the mechanisms of action in this context have not been entirely elucidated. The present study aimed to evaluate the effects of myrrh, coffee charcoal and chamomile flower extract on the inflammatory cross talk between immune and intestinal epithelial cells together with the resulting intestinal barrier disorders. A complex co-culture cell model consisting of intestinal epithelial cell (IEC) monolayers (Caco-2, HT29-MTX-E12) and macrophages (THP-1) was established for the simultaneous investigation of these two IBD characteristics. The lipopolysaccharide (LPS) activation of the macrophages led to a pro-inflammatory mediator release and thereby an inflammatory stimulation of IECs with chemokine release and reduced barrier function. The effects of the individual plant extracts and a ternary combination on inflammatory mediator release (IL-6, TNF, IL-8, MCP-1, PGE2) was quantified by ELISA. The transepithelial electrical resistance (TEER) of IEC monolayers was measured to evaluate the effects on the barrier function. Budesonide served as a positive control. All three plant extracts exhibited anti-inflammatory properties via the inhibition of the inflammatory mediator release to a varying extent. An intestinal barrier stabilising effect was observed for myrrh and coffee charcoal. Myrrh exerted the most distinct pharmacological activity. Dose reducing and synergistic interactions emerged within the threefold combination. Thus, our results provide a mechanistic basis for the use of the herbal combination of myrrh, coffee charcoal and chamomile flower extract in IBD treatment and underline the potential benefits of the phytotherapeutic multi-component/multi-target approach in this complex pathogenesis.
Inhibition of Self-incompatible Pollen in Papaver rhoeas Involves a Complex Series of Cellular Events

Self-incompatibility (SI) in Papaver rhoeas involves allele-specific recognition between stigmatic S proteins and pollen which results in the rapid inhibition of incompatible pollen. In this article we discuss current understanding of the components and mechanisms involved in this reaction. Analysis of cDNA sequences encoding several stigmatic S alleles has allowed us to establish, using site-directed mutagenesis, that certain amino acids are essential for S -specific inhibitory activity. An S protein binding protein (SBP) identified in pollen plasma membrane extracts has been proposed to act as a receptor. Study of S1gene mutants defective in SBP binding has confirmed the important role of SBP in the SI reaction, however the exact nature of this remains to be defined. Although it was previously thought that the Papaver stigmatic S gene was unique, detailed analysis of Arabidopsis thaliana genomic sequences has revealed the presence of a large number of open reading frames with homology to the S gene. We have named these potential genes SPH(S-protein homologues). Studies of the molecular and biochemical basis of self-incompatibility (SI) in P. rhoeas have revealed much about the early signals triggered in pollen in this response. We have established that a Ca2+-dependent signal transduction pathway mediates the inhibition of incompatible pollen. Rapid increases in cytosolic free Ca2+and the rapid loss of the pollen apical Ca2+gradient are accompanied by the hyper-phosphorylation of p26 and p68, two soluble pollen phosphoproteins. Studies reveal that p26 is a soluble inorganic pyrophosphatase. Recent evidence has been obtained that suggests irreversible pollen tube growth and cell death involves a programmed cell death (PCD) pathway.
Advanced Diabetes Management Using Artificial Intelligence and Continuous Glucose Monitoring Sensors

Wearable continuous glucose monitoring (CGM) sensors are revolutionizing the treatment of type 1 diabetes (T1D). These sensors provide in real-time, every 1–5 min, the current blood glucose concentration and its rate-of-change, two key pieces of information for improving the determination of exogenous insulin administration and the prediction of forthcoming adverse events, such as hypo-/hyper-glycemia. The current research in diabetes technology is putting considerable effort into developing decision support systems for patient use, which automatically analyze the patient’s data collected by CGM sensors and other portable devices, as well as providing personalized recommendations about therapy adjustments to patients. Due to the large amount of data collected by patients with T1D and their variety, artificial intelligence (AI) techniques are increasingly being adopted in these decision support systems. In this paper, we review the state-of-the-art methodologies using AI and CGM sensors for decision support in advanced T1D management, including techniques for personalized insulin bolus calculation, adaptive tuning of bolus calculator parameters and glucose prediction.
Inhibition of NF-κB Signaling Alters Acute Myelogenous Leukemia Cell Transcriptomics

Acute myelogenous leukemia (AML) is an aggressive hematological malignancy. The pathophysiology of the disease depends on cytogenetic abnormalities, gene mutations, aberrant gene expressions, and altered epigenetic regulation. Although new pharmacological agents have emerged during the last years, the prognosis is still dismal and new therapeutic strategies are needed. The transcription factor nuclear factor-κB (NF-κB) is regarded a possible therapeutic target. In this study, we investigated the alterations in the global gene expression profile (GEP) in primary AML cells derived from 16 consecutive patients after exposure to the NF-κB inhibitor BMS-345541. We identified a profound and highly discriminative transcriptomic profile associated with NF-κB inhibition. Bioinformatical analyses identified cytokine/interleukin signaling, metabolic regulation, and nucleic acid binding/transcription among the major biological functions influenced by NF-κB inhibition. Furthermore, several key genes involved in leukemogenesis, among them RUNX1 and CEBPA, in addition to NFKB1 itself, were influenced by NF-κB inhibition. Finally, we identified a significant impact of NF-κB inhibition on the expression of genes included in a leukemic stem cell (LSC) signature, indicating possible targeting of LSCs. We conclude that NF-κB inhibition significantly altered the expression of genes central to the leukemic process.
Maintenance requirements of phosphorus and absorbability of dietary phosphorus in sheep

The factorial approach to the determination of the dietary requirements of macroelements is a two-stage process; in the first the net requirements are estimated and in the second, the fractional absorption of the dietary mineral is used to convert net to dietary requirements (Agricultural Research Council, 1965). At the first stage the maintenance component is allotted a fixed value, so that estimated dietary requirements are directly proportional to absorbability of the element.
Recurrent hospitalizations are associated with increased mortality across the ejection fraction range in heart failure

The proportion of patients hospitalized for heart failure (HF) with preserved left ventricular ejection fraction (LVEF) is rising, but no approved treatment exists, in part owing to incomplete characterization of this particular HF phenotype. In order to better define the characteristics of HF phenotypes in Finland, a large cohort with 12 years' follow‐up time was analysed.
Dietary Selenium Intakes and Musculoskeletal Function in Very Old Adults: Analysis of the Newcastle 85+ Study

Background: Selenium is a trace element essential for health. Severe selenium deficiencies are associated with poor musculoskeletal (MSK) function. However, the effects of moderate deficiency on MSK function, especially in older adults, is unclear. Objectives: To determine the associations between selenium intake and MSK function in very old adults. Methods: Selenium intake at baseline and, hand-grip strength (HGS) and timed-up-and-go (TUG) at four phases over 5 years, were available in 791 participants in the Newcastle 85+ Study, a community-based, longitudinal cohort of ≥ 85 year old individuals. We investigated relationships between selenium intake and HGS and TUG in cross-sectional analyses at baseline using multivariate analyses and, prospectively using linear mixed models to explore HGS and TUG changes over 5 years in association with baseline selenium intake. Results: At baseline, 53% of participants had selenium intakes that were classified as low. These individuals had 2.80 kg lower HGS and were 2.30 s slower performing the TUG, cross-sectionally. In multivariate, baseline analyses, selenium intake had no significant impact on HGS or TUG. Selenium intake had no significant effect on MSK function, prospectively. Conclusion: Low selenium intake is common among very old adults and, in cross-sectional analyses, is associated with poorer MSK function.
Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC

Background: The Anaplastic Lymphoma Kinase (ALK) gene is known to be affected by several genetic alterations, such as rearrangement, amplification and point mutation. The main goal of this study was to comprehensively analyze ALK amplification (ALK-A) and ALK gene copy number gain (ALK-CNG) in a large cohort of non-small-cell lung cancer (NSCLC) patients in order to evaluate the effects on mRNA and protein expression. Methods: ALK locus number status was evaluated in 578 NSCLC cases by fluorescence in situ hybridization (FISH). In addition, ALK immunohistochemistry and ALK mRNA in situ hybridization were performed. Results: Out of 578 cases, 17 cases showed ALK-A. In addition, 14 cases presented ALK-CNG and 72 cases presented chromosome 2 polyploidy. None of those carrying ALK-A and -CNG showed either ALK immunohistochemical expression or ALK mRNA expression through in situ hybridization. We observed a high frequency of extra copies of the ALK gene. Conclusions: Our findings demonstrated that ALK-A is not involved in mRNA production and consequently is not involved in protein production; these findings support the hypothesis that ALK-A might not play a role in the pathogenesis of NSCLC, underlining the absence of a specific clinical application.
Impacts of plant roots on soil CO cycling and soil-atmosphere CO exchange

Carbon monoxide (CO) plays a major role in tropospheric chemical dynamics. Accordingly, global CO budgets have been reasonably well documented. Atmospheric CO consumption by soils contributes significantly to these budgets, with the magnitude of the sink generally considered to reflect a balance between microbial uptake and abiological production. However, assays of live fine roots showed that diverse intact plants produced carbon monoxide at net rates ranging from 2 to 3000 µg gdw -1 d -1 . C O production was greater for legumes than nonlegumes, and primarily associated with nodules. Excised roots from woody and herbaceous plants produced CO at comparable rates. CO production rates were similar for roots of intact plants and roots excised from those plants. The magnitude of net CO fluxes from roots was determined in part by the balance between simultaneous production and consumption. Surface sterilization of roots indicated that CO consumption was due, in part, rhizoplane CO­oxidizing bacteria, but maximum CO consumption rates were typically only a small fraction of net produc­ tion rates. Assays in a Brazilian agroecosystem indicated that root CO production affects soil-atmosphere CO exchange. Estimates of global CO production rates indicated that roots contribute about 170-260 Tg CO to the soil atmosphere annually, an amount com­ parable to current estimates of atmospheric CO uptake by soils, and much larger than estimates of net abiological soil CO production.
Dietary habits associated with the presence of organochlorine pesticides in human milk

Abstract This study aims to establish the relationship between the consumption of certain foods and the presence of organochlorine pesticides in human milk. A total of 167 samples of breastmilk were collected. Sample analysis was carried out by gas chromatography with electron capture detector. Pesticide concentrations (op’DDT, pp’DDT, pp’DDE, Σ-DDT, HCB, β-HCH) were grouped by consumption level of fish, dairy products, meat, eggs, and seafood and then were compared to each other. The odds ratio (OR) was calculated between the concentration of pesticides (higher and/or lower than median) and the exposure variable (high consumption). The results indicated low median concentrations of HCB, β − HCH, op’DDT and pp’DDT, whose fluctuations and trends between different frequencies of food consumption were not significant. pp’DDE and Σ − DDT values according to the increase in consumption of fish were significantly increased. Women with high fish consumption have a higher risk of high concentrations of pp’DDE and Σ-DDT (OR: high consumption: 5.6 (1.3–23.6). A protective effect was observed in the consumption of dairy products (β-HCH and op’DDT), meat (HCB), and seafood (pp’DDT). These results suggest that it is possible that the consumption of fish, dairy products, meat, and seafood influences the presence of organochlorine pesticides in the studied population.
Nickel accumulation by Colocassia esculentum and its impact on plant growth and physiology

The remediation of heavy metal-contaminated sites using plants presents a promising alternative to current methodologies. In this study, the potential of Colocassia esculentum for Nickel (Ni) accumulation was determined. C. esculentum plants exposed to Ni, demonstrated capability to accumulate on average, more in shoots as compared to roots, suggesting better translocation of Ni from root to shoot. High metal content in soil caused reduction in growth parameters and an increase in oxidative stress. Under heavy metal stress, an increase in catalase, peroxidase, ascorbic acid and proline were observed in the roots along with some anatomical changes. Lipid peroxidation showed a slight increase by Ni treatment along with some anatomical changes in the root. This work demonstrates that metal induced oxidative stress occurs by the presence of heavy metals at higher concentrations. It also suggests that superior antioxidative defenses, particularly catalase activity, may play an important role in C. esculentum. The outcome of this study corroborate that C. esculentum is a suitable candidate for the phytoremediation of Ni contaminated soil and could be considered as a potential Ni hyperaccumulator plant species.