Miracle-dz/UniFAR-FTUs huggingface dataset:
Interplay between Humoral and CLA+ T Cell Response against Candida albicans in Psoriasis

Candida albicans (CA) infections have been associated with psoriasis onset or disease flares. However, the integrated immune response against this fungus is still poorly characterized in psoriasis. We studied specific immunoglobulins in plasma and the CA response in cocultures of circulating memory CD45RA− cutaneous lymphocyte antigen (CLA)+/− T cell with autologous epidermal cells from plaque and guttate psoriasis patients (cohort 1, n = 52), and also healthy individuals (n = 17). A complete proteomic profile was also evaluated in plaque psoriasis patients (cohort 2, n = 114) regarding their anti-CA IgA levels. Increased anti-CA IgA and IgG levels are present in the plasma from plaque but not guttate psoriasis compared to healthy controls. CA cellular response is confined to CLA+ T cells and is primarily Th17. The levels of anti-CA IgA are directly associated with CLA+ Th17 response in plaque psoriasis. Proteomic analysis revealed distinct profiles in psoriasis patients with high anti-CA IgA. C-C motif chemokine ligand 18, chitinase-3-like protein 1 and azurocidin were significantly elevated in the plasma from plaque psoriasis patients with high anti-CA levels and severe disease. Our results indicate a mechanism by which Candida albicans exposure can trigger a clinically relevant IL-17 response in psoriasis. Assessing anti-CA IgA levels may be useful in order to evaluate chronic psoriasis patients.
Gaze Following and Pupil Dilation as Early Diagnostic Markers of Autism in Toddlers

Background: Children with autism spectrum disorder (ASD) show certain characteristics in visual attention. These may generate differences with non-autistic children in the integration of relevant social information to set the basis of communication. Reliable and objective measurement of these characteristics in a language learning context could contribute to a more accurate early diagnosis of ASD. Gaze following and pupil dilation are being studied as possible reliable measures of visual attention for the early detection of ASD. The eye-tracking methodology allows objective measurement of these biomarkers. The aim of this study is to determine whether measurements of gaze following and pupillary dilation in a linguistic interaction task are potential objective biomarkers for the early diagnosis of ASD. Method: A group of 20 children between 17 and 24 months of age, made up of 10 neurotypical children (NT) and 10 children with an increased likelihood of developing ASD were paired together according to chronological age. A human face on a monitor pronounced pseudowords associated with pseudo-objects. Gaze following and pupil dilation were registered during the task These measurements were captured using eye-tracking methodology. Results: Significant statistical differences were found in the time of gaze fixation on the human face and on the object, as well as in the number of gazes. Children with an increased possibility of developing ASD showed a slightly higher pupil dilation than NT children. However, this difference was not statistically significant. Nevertheless, their pupil dilation was uniform throughout the different periods of the task while NT participants showed greater dilation on hearing the pseudoword. Conclusions: The fixing and the duration of gaze, objectively measured by a Tobii eye-tracking system, could be considered as potential biomarkers for early detection of ASD. Additionally, pupil dilation measurement could reflect differential activation patterns during word processing in possible ASD toddlers and NT toddlers.
The Nrf2 pathway in psychiatric disorders: pathophysiological role and potential targeting

ABSTRACT Introduction: All psychiatric disorders exhibit excitotoxicity, mitochondrial dysfunction, inflammation, oxidative stress, and neural damage as their common characteristic. The endogenous nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway is implicated in the defense mechanism against oxidative stress and has a significant role in psychiatric disorders. Areas covered: We explore the role of Nrf2 pathway and its modulators in psychiatric disorders. The literature was searched utilizing various databases such as Embase, Medline, Web of Science, Pub-Med, and Google Scholar from 2010 to 2020. The search included research articles, clinical reports, systematic reviews, and meta-analyses. Expert opinion: Environmental factors and genetic predisposition can be a trigger for the development of psychiatric disorders. Nrf2 downregulates certain inflammatory pathways and upregulates various antioxidant enzymes to maintain a balance. However, its intricate balance with NF-Kβ (Nuclear factor kappa light chain enhancer of activated B cells) and its crosstalk with the transcription factor Nrf2 is critical in severe oxidative stress. Several Nrf2 modulators are now in clinical trials and can help reduce oxidative stress and neuroinflammation. There are immense potential opportunities for these modulators to become a novel therapeutic option. Graphical Abstract
Platelet-Rich Fibrin in Single and Multiple Coronally Advanced Flap for Type 1 Recession: An Updated Systematic Review and Meta-Analysis

Background and Objectives: The aim of the present systematic review and meta-analysis was to investigate the efficacy of leukocyte–platelet-rich fibrin (L-PRF) in addition to coronally advanced flap (CAF) for the treatment of both single and multiple gingival recessions (GRs) compared to the CAF alone and to the adjunct of connective tissue graft (CTG). Root coverage outcomes using platelet concentrates have gained increased interest. In particular, it has been suggested that adding L-PRF to CAF may provide further benefits in the treatment of GRs. Materials and Methods: An electronic and manual literature search was conducted to identify randomized controlled trials (RTCs) investigating root coverage outcomes with CAF + L-PRF. The outcomes of interest included mean root coverage (mRC), recession reduction, keratinized tissue width (KTW) gain, gingival thickness (GT) gain, and patient-reported outcome measures (PROms) such as pain perception and discomfort. Results: A total of 275 patients and 611 surgical sites were analyzed. L-PRF in adjunct to single CAF seems to show statistically significant results regarding clinical attachment level (CAL) with a weighted means (WM) 0.43 95% CI (−0.04, 0.91), p < 0.0001, GT (WM 0.17 95% CI (−0.02, 0.36), p < 0.0001, and mRC (WM 13.95 95% CI (−1.99, 29.88) p < 0.0001, compared to single CAF alone. Interesting results were obtained from the adjunct of PRF to multiple CAF with respect to multiple CAF alone with an increase in the mRC WM 0.07 95% CI (−30.22, 30.35), p = 0.0001, and PPD change WM 0.26 95% CI (−0.06, 0.58), p < 00001. On the other hand, no statistically significant data were obtained when L-PRF was added to single or multiple CAF combined with CTG according to the included outcomes such as mRC (p = 0.03 overall). Conclusions: L-PRF is a valid alternative to CAF alone. L-PRF compared to CTG in single and multiple CAF showed statistically significant results regarding pain perception and discomfort PROms (p < 0.0001). However, CTG remains the gold standard for treating gingival recession.
Sulfur Species Investigation in Extra- and Intracellular Sulfur Globules of Acidithiobacillus ferrooxidans and Acidithiobacillus caldus

The sulfur chemical speciation in extracellular and intracellular sulfur globules of Acidithiobacillus ferrooxidans and Acidithiobacillus caldus were investigated with an integrated approach including scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy and sulfur K-edge X-ray absorption near edge structure spectroscopy (XANES). The results indicated that both strains can accumulate extracellular sulfur globules when grown on thiosulfate, and the major sulfur chemical speciation of which were S8 for A. ferrooxidans and mixture of ring sulfur and polythionate for A. caldus, respectively. In contrast, A. ferrooxidans can accumulate both linear sulfur and S8 internally when grown with sulfur powder and thiosulfate, whereas A. caldus did not accumulate intracellular sulfur globules. In addition, the fitted results of sulfur K-edge XANES spectra indicated that the reduced glutathione (containing thiols groups) were involved in sulfur bio-oxidation of both strains and the tetrathionate were the intermediate products during thiosulfate metabolism by two strains.
Efficacy of supplemental multi-carbohydrases in broiler diets depends on soluble arabinoxylan-to-total arabinoxylan content

ABSTRACT 1. The objective of this study was to investigate the effect of multi-carbohydrase enzymes (MC) on net energy (NE), performance and gene expression in Cobb 500 broilers fed diets containing different levels of soluble and total arabinoxylan (sAX/tAX) ratios. 2. The study employed a 2 × 3 factorial arrangement of treatments, with factors including with or without MC and three ratios of sAX/tAX: high (HS, 27.3%), intermediate (IS, 21.3%) and low (LS, 15.7%). 3. Six dietary treatments were formulated, with each diet replicated five times in the calorimetric study (Experiment 1) and eight times for a floor pen feeding trial (Experiment 2). 4. Experiment 1 showed significant (P < 0.01) MC × sAX/tAX interactions for apparent metabolisable energy (AME) and NE. These interactions indicated that the supplemental MC increased AME only in the HS diet, and NE in the HS and LS diets. 5. Experiment 2 results showed MC × sAX/tAX interactions for feed conversion ratio (FCR, P < 0.01) and ileal digesta viscosity (P < 0.05), demonstrating that MC lowered FCR only in the LS-fed birds, and reduced digesta viscosity only in the HS-fed birds. Apparent ileal digestible crude protein (AID CP) was negatively correlated with ileal digesta viscosity (r = −0.735, P < 0.001), which suggested that increasing ileal digesta viscosity reduced AID CP. 6. A significant (P < 0.05) MC × sAX/tAX interaction was observed for duodenal COX III mRNA gene expression, which indicated that this gene was upregulated in the IS-fed birds relative to the HS-fed birds, but only when MC was added. This gene was downregulated (P < 0.05) in the muscle in the presence of MC application in all diets. 7. The results from this study showed that supplemental MC can improve NE and FCR in birds fed diets containing the low sAX/tAX ratios.
Role of dominant macrophytes to treat Nambul river, the main polluter of Loktak – a dying Ramsar site in the Indo Burma hot spot (Manipur, India)

Abstract As a part of the monitoring process of the main polluting tributary of Loktak, the paper analyzed the water characteristics of the main polluted stretch of Nambul river i.e. Imphal Urban region. The results showed low pH (6.23 ± 0.32), high electrical conductivity (1373 ± 50.33 µSm−1), high turbidity (25.40 ± 0.90 NTU), poor dissolved oxygen content (0.53 ± 0.24 mgL−1), high nitrate, and phosphate concentrations (9.27 ± 0.40 and 2.05 ± 0.10 mgL−1, respectively). The Water Quality Index values indicate that Nambul river is unfit for drinking. Moreover, the Sodium Percentage, Sodium Adsorption Ratio, and Kelly’s Ratio revealed the unsuitable nature of the river for irrigation. Therefore, in order to clean the river water, lab scale microcosm experimentation was carried out to highlight the potential of the dominant macrophytes viz. Eichhornia crassipes (Mart) Solms and Pistia stratiotes (L.) as phytofilters. The results showed increased pH (7.6 ± 0.11 and 7.5 ± 0.1), higher dissolved oxygen content (5.87 ± 0.30 and 3.28 ± 0.30 mgL−1), less turbidity (0.60 ± 0.25 and 0.40 ± 0.15 NTU) and reduced nitrate and phosphate concentrations (0.37 ± 0.03 and 0.52 ± 0.01 mgL−1, and 0.534 ± 0.025 and 0.346 ± 0.01 mgL−1, respectively). Novelty statement The present study showed the deteriorating water quality of Nambul river, the main polluting source of Loktak – a Ramsar site in the Montreux records. Water Quality Index calculated was above the unsuitable level for drinking at all the sampling locations in the Imphal Urban region. Sodium percentage, sodium adsorption ratio and Kelly’s ratio were above the desired limit, making the river water unfit for irrigation. Further, the microcosm experimentation showed that the dominant macrophytes viz. Eichhornia crassipes (Mart) Solms and Pistia stratiotes (L.) can control the pollution level of Nambul.
Effects of tea tree oil supplementation on growth performance, antioxidant capacity, immune status and microbial community in weaned pigs

ABSTRACT The objective of this study was to determine whether dietary tea tree oil (TTO) supplementation could effectively replace the antibiotics through modulating the antioxidant capacity and intestinal microbiota profile, and then decreasing the diarrhoea incidence and improving the growth performance of weaned pigs. A total of 216 weaned pigs with initial body weights (BW) of 9.19 ± 1.86 kg were randomly allocated to three dietary treatments in a completely randomised design. The dietary treatments included a corn-soybean meal basal diet (CON) without any antibiotics, and two experimental diets formulated by adding 75 mg/kg aureomycin (AGP) or 100 mg/kg TTO into the basal diet, respectively. Pigs fed the TTO diet showed greater gain to feed ratio (p < 0.05) than those fed CON and AGP diets during d 0–14 and d 14–28. Both dietary TTO and AGP supplementation tended to increase the average daily gain of weaned pigs during d 14–28 (p = 0.06) and the overall 28-d period (p = 0.07), and significantly reduced (p < 0.05) the diarrhoea incidence during d 0–14 compared with the CON treatment. In addition, dietary TTO supplementation improved the apparent total tract digestibility of dry matter and ether extract (p < 0.05), and increased (p < 0.05) the propionate and butyrate concentrations in faecal samples of weaned pigs. Moreover, pigs fed the TTO diet showed greater total antioxidant capacity, greater superoxide dismutase and interleukin-10 concentrations, and lower malondialdehyde concentration in serum than those fed the CON diet (p < 0.05). Furthermore, pigs fed the TTO diet demonstrated greater relative abundance of Clostridiaceae_1, while those fed the AGP diet exhibited greater relative abundance of Lactobacillaceae at family level. In conclusion, dietary TTO supplementation could improve growth performance in weaned pigs, which could be mainly attributed to the benefits on nutrient digestibility, antioxidative capacity and microbial community profile.
Surgical mask covering of N95 filtering facepiece respirators: The risk of increased leakage

In this report, we demonstrate the potential risk of increased face-to-mask seal leakage when N95 filtering facepiece respirators (N95 FFRs) are covered by surgical, cloth, or medical masks, (collectively referred to as surgical masks), through analytical modeling of the associated fluid mechanics and seal pressures. Previously published experimental studies of respirator pressures and leakage are applicable to this problem. Properly utilized N95 FFRs will remain an essential component of healthcare worker safety for the foreseeable future, especially for those engaged in aerosolgenerating procedures (AGPs) such as endotracheal intubation.1–3 When considering leakage risk, it is important to understand the general challenges to ensuring an adequatemask-to-face seal. The fit and seal degrade with repeated donning and doffing, and some N95 FFR reprocessing or recycling techniques have been reported to accelerate this degradation.4 In short, the N95 mask-to-face seal is fragile and can be compromised by a number of factors.
Sprint Tactics in the Tour de France: A Case Study of a World-Class Sprinter (Part II).

PURPOSE To describe the performance and tactical sprint characteristics of a world-class sprinter competing in the Tour de France. In addition, differences in the sprint tactics of 2 teams and won versus lost sprints are highlighted. METHOD Power output (PO) and video footage of 21 sprints were analyzed. Position in the peloton and number of teammates supporting the sprinter at different times before the finish line together with PO for different time intervals were determined. Sprints were classified as team Shimano (2013-2014) and team Quick-step (2016-2017), as well as won or lost. RESULTS The sprinter was highly successful, winning 14 out of the 21 sprints. At time intervals 10 to 5, 3 to 2, and 1.5 to 1 minute, POs were significantly lower in team Quick-step compared with team Shimano, but the sprinter was positioned further away from the front at 10, 2, 1.5, 1, and 0.5 minutes at team Quick-step compared with team Shimano. The PO was higher at time interval 0.5 to 0.25 minutes before the finish line with team Quick-step when compared with team Shimano. The position of the sprinter in the peloton in lost sprints was further away from the front at 0.5 minutes before the finish compared with won sprints, while no differences were noted for PO and the number of teammates between won and lost sprints. CONCLUSIONS Differences in sprint tactics (Shimano vs Quick-step) influence the PO and position in the peloton during the sprint preparation. In addition, the position at 0.5 minutes before the finish line influences the outcome (won or lost) of the sprint.
Plant-Based Alternatives to Yogurt: State-of-the-Art and Perspectives of New Biotechnological Challenges

Due to the increasing demand for milk alternatives, related to both health and ethical needs, plant-based yogurt-like products have been widely explored in recent years. With the main goal to obtain snacks similar to the conventional yogurt in terms of textural and sensory properties and ability to host viable lactic acid bacteria for a long-time storage, several plant-derived ingredients (e.g., cereals, pseudocereals, legumes, and fruits) as well as technological solutions (e.g., enzymatic and thermal treatments) have been investigated. The central role of fermentation in yogurt-like production led to specific selections of lactic acid bacteria strains to be used as starters to guarantee optimal textural (e.g., through the synthesis of exo-polysaccharydes), nutritional (high protein digestibility and low content of anti-nutritional compounds), and functional (synthesis of bioactive compounds) features of the products. This review provides an overview of the novel insights on fermented yogurt-like products. The state-of-the-art on the use of unconventional ingredients, traditional and innovative biotechnological processes, and the effects of fermentation on the textural, nutritional, functional, and sensory features, and the shelf life are described. The supplementation of prebiotics and probiotics and the related health effects are also reviewed.
Microbiota-derived short-chain fatty acids do not interfere with SARS-CoV-2 infection of human colonic samples

ABSTRACT Microbiota-derived molecules called short-chain fatty acids (SCFAs) play a key role in the maintenance of the intestinal barrier and regulation of immune response during infectious conditions. Recent reports indicate that SARS-CoV-2 infection changes microbiota and SCFAs production. However, the relevance of this effect is unknown. In this study, we used human intestinal biopsies and intestinal epithelial cells to investigate the impact of SCFAs in the infection by SARS-CoV-2. SCFAs did not change the entry or replication of SARS-CoV-2 in intestinal cells. These metabolites had no effect on intestinal cells’ permeability and presented only minor effects on the production of anti-viral and inflammatory mediators. Together our findings indicate that the changes in microbiota composition of patients with COVID-19 and, particularly, of SCFAs do not interfere with the SARS-CoV-2 infection in the intestine.
Switchable Coupled Relays Aid Massive Non-Orthogonal Multiple Access Networks with Transmit Antenna Selection and Energy Harvesting

The article proposes a new switchable coupled relay model for massive MIMO-NOMA networks. The model equips a much greater number of antennas on the coupled relays to dramatically improve capacity and Energy Efficiency (EE). Each relay in a coupled relay is selected and delivered into a single transmission block to serve multiple devices. This paper also plots a new diagram of two transmission blocks which illustrates energy harvesting and signal processing. To optimize the system performance of a massive MIMO-NOMA network, i.e., Outage Probability (OP) and system throughput, this paper deploys a Transmit Antenna Selection (TAS) protocol to select the best received signals from the pre-coding channel matrices. In addition, to achieve better EE, Simultaneously Wireless Information Power Transmit (SWIPT) is implemented. Specifically, this paper derives the novel theoretical analysis in closed-form expressions, i.e., OP, system throughput and EE from a massive MIMO-NOMA network aided by switchable coupled relays. The theoretical results obtained from the closed-form expressions show that a massive MIMO-NOMA network achieves better OP and greater capacity and expends less energy than the MIMO technique. Finally, independent Monte Carlo simulations verified the theoretical results.
Using Narratives to Correct Forecasting Errors in Pediatric Tracheostomy Decision Making

Purpose Parents who face goals-of-care tracheostomy decisions may lack an understanding of challenges affecting their child’s and family’s long-term quality of life (QOL) to accurately forecast possible outcomes for decision making. We sought to examine whether and how parents’ narratives of the child’s and family’s long-term QOL influence parental tracheostomy decisions and forecasting. Method We recruited US adult Amazon Mechanical Turk participants (N = 1966) who self-reported having a child (<6 y old) or planning a pregnancy within 5 y. Participants read a vignette about making a tracheostomy decision for their hypothetical neurologically impaired baby. They were randomized to 1 of the following 4 conditions: 1) Baby QOL narratives, 2) Family QOL narratives, 3) Baby QOL + Family QOL narratives, and 4) control: no narratives. They then made a decision about whether or not to pursue tracheostomy, forecasted their concerns about the baby’s and family’s QOL, reported their values and social norm beliefs about tracheostomy, comfort care, and parental medical decision making, and completed individual differences scales and demographics. Results Controlling for individual characteristics, participants in the Baby QOL and Baby QOL + Family QOL conditions were less likely to choose tracheostomy as compared with the control (odds ratio [OR] = 0.38 and 0.25, respectively, P < 0.001). Fewer participants in the Family QOL condition chose tracheostomy compared with the control, but this difference was not statistically significant (OR = 0.70, P = 0.11). Moreover, narratives increased pessimistic forecasting, which was associated with less interest in tracheostomy. Conclusion Narratives clarifying long-term implications of pursuing tracheostomy have the potential to influence forecasting and decisions. Narrative-based interventions may be valuable in other situations in which forecasting errors are common.