Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Mechanisms In Cftr-F508del Degradation And The Functional Defect Of Cftr Absence In Rabbits

MOLECULAR MECHANISMS IN CFTR-F508DEL DEGRADATION AND THE FUNCTIONAL DEFECT OF CFTR ABSENCE IN RABBITS by CARTHIC RAJAGOPALAN August 2019 Advisors: Dr. Xuequn Chen Major: Physiology Degree: Doctor of Philosophy Cystic Fibrosis (CF) is the most common, lethal autosomal recessive disorder, and is caused by mutations in the cystic fibrosis transmembrane conductance regulator protein (CFTR), an anion channel that is found in most epithelial cells lining the airway and gut. The most common mutation of CFTR is deletion of phenylalanine at position 508 (CFTR-F508del), which produces a misfolded protein. Through the ubiquitin proteasome system (UPS), this misfolded protein is ubiquitinated and signaled for degradation via the cytosolic proteasome. Previous studies demonstrating experimental restoration of F508DEL-CFTR trafficking to the plasma membrane showed partial function of the chloride channel, raising therapeutic speculations. Some components of how F508DEL-CFTR is degraded is known, however many mechanisms that underlie its degradation through ERAD and the UPS is still unknown. In the first part of the study we used a siRNA screen to discover the E3 ligase, RNF19B, and its interacting partner, UBE 2L6 mediates F508del degradation. We used siRNA-mediated silencing of endogenous UBE 2L6 and RNF19B in the CF human bronchial epithelial (HBE) cell line to demonstrate that there is an increase in F508del-CFTR expression. We also co-expressed UBE 2L6 and RNF 19B with F508del in HEK293 cells that demonstrated a decrease in F508del compared to control.
PCAOB Inspections and the Financial Reporting Quality of Complex Financial Instruments

We examine whether fair value (FV) deficiencies highlighted in Public Company Accounting Oversight Board (PCAOB) inspection reports of Big 4 audit firms improve the quality of fair value reporting for the auditor’s portfolio of clients. Since prior literature finds positive consequences from inspection reports, we might expect those positive associations to extend to the fair value setting. However, qualitative studies show the challenges auditors face in providing positive assurance on complex financial instruments, while Glover et al. (2019) suggest that differences in opinion between auditors and PCAOB inspectors about what constitutes a fair value audit deficiency may hinder improvements in the fair value audit process. Using a marketbased measure of financial reporting quality (i.e., implied asset-specific betas related to FV disclosures), we find evidence that the intensity of FV deficient reports is positively associated with the quality of fair value financial reporting and that the association is driven by audit clients facing the greatest exposure to fair value assets. We utilize a measure of office-level FV audit expertise and find that our results are also driven by the clients of Big 4 audit offices with high levels of FV expertise. Our evidence is consistent with PCAOB inspections and auditors playing a critical role in improving the quality of the fair value audit process and financial reporting. Data Availability: All data are publicly available as described in the text.
Human Papillomavirus Infection and Associated Risk Factors in Indigenous and Urban Women from Cañar Province, Ecuador

Two groups of Ecuadorian women were included, indigenous from rural region and urban women, both from Canar province. A descriptive cross-sectional study was conducted from July 2017 to September 2018. Two hundred endocervical cytological samples were collected from both women populations between 15 and 55 years of age, residing in this province. Samples were analyzed by Papanicolaou test and mucosal cells were submitted to DNA extraction and subsequently HPV detection and genotyping using a commercial kit CLART® HPV 2 (Genomic, Spain).
Running Head: CONSULTANCY UNDER PRESSURE 1 Consultancy Under Pressure: Intervening in the ‘here and now’ with an Elite Golfer. CONSULTANCY UNDER PRESSURE

This case study is a reflective account of a consultation with a 30-year-old male professional golfer. The approach by the player was made on the evening prior to the final round of a European Tour event, needing a top 20 finish on the final day of the tournament year. Failure to achieve this objective would have resulted in forfeiting of his playing privileges on the PGA European Tour for the following season, with the associated loss of income, and in this case genuine threats to the livelihood of the client. The consultant used a number of interventions (e.g., best performance imagery, an external focus of attention, hypnosis and music) and an approach established in humanistic, phenomenological and transpersonal psychology. Effectiveness was determined by performance and the players' descriptions of his transcendental experience. The client provided social validation for the consultation approach and the intervention through his reflections. The intervention in this case appeared to elevate positive emotions and trigger a transcendental precursor to peak performance. While this type of intervention may provide immediate performance benefits for golfers experiencing low self-confidence, the case-study also illustrates how consultants are often asked to support athletes within severe time constraints.
Volume 40, Issue 4 Is information and communication technology a driver of industrialization process in African countries?

This study investigates how information and communication technology affects industrialization process in 46 African countries over the period 2000-2015. Two information and communication technology indicators (consisting of internet penetration and Mobile phone penetration), and two industrialization indicators (entailing manufacturing value added and employment in industry) are used. The empirical evidence is based on Ordinary Least Squared (OLS), fixed effects (FE) and system Generalized Method of Moments (GMM). The results show that ICT has a positive and significant effect on industrialization in African countries, even if this effect is tiny
On comparing BJH and NLDFT pore-size distributions determined from N2 sorption on SBA-15 substrata

SBA-15 silica materials consisting of a collection of non-intersecting cylindrical pores of varying diameters have been utilized to try to reconcile the pore-size distribution results proceeding from the classical Barrett–Joyner–Halenda (BJH) and modern non-local density functional theory (NLDFT) approaches. To assess such pretended concordance, it is necessary to perform BJH pore-size estimates on the basis of a modified Kelvin equation that makes allowance for the adsorption potential field emanating from the solid walls of the adsorbent towards the adsorbate molecules. Under this context, critical conditions for capillary condensation and evaporation to happen in cylindrical pores have been specifically calculated via a treatment previously developed by Broekhoff and de Boer (BdB). In this way, BJH-BdB pore-size distribution results, obtained from the analyses of both ascending and descending boundary curves of N2 sorption isotherms at 76 K on a series of model SBA-15 substrata that have been synthesized in this work, are compared with homologous curves proceeding from a NLDFT treatment performed on the descending boundary curve and very reasonable agreement has been found.
Predicting the Winner of a Tennis Match using Machine Learning Techniques

Winning is the primary goal of any sport. Predicting the winner of the match in advance has gained a lot of attention by sports organizations and potential bidders as it involves a lot of time and money invested in it. Now-a-days, sports organizations realize the value of data and the science in the data which can be used as an advantage to players, coaches also the potential bidders using machine learning techniques. Tennis is a challenging and unpredictable sport, yet the most exciting sport which is enjoyed by fans from all over the world. Machine learning techniques have helped in predicting the outcomes of tennis matches using various attributes. Removing the irrelevant attribute plays a very important role in getting high accuracy hence in this research we have used the Principle component analysis (PCA) for dimensioanlity reduction and the machine learning classifiers such as SVM, Naive Bayes, Logistic regression and Random forest are used to forecast the winner of the match. The hyper-parameter tuning is performed to highlight the most significant parameters which help in increasing the accuracy of the models used. These models are finally evaluated in terms of accuracy, F1 score, Cohens kappa and AUC. The results show that after hyper parameter tuning Random Forest with F1 score has outperformed all other models with 78% accuracy.
Effect Of Zataria Multiflora (Avishan-E- Shirazi) In Helicobacter Pylori Eradication

Introduction: Drug resistance has increased the failure of H-pylori eradication therapy worldwide, therefore it is necessary to examine newer treatment regimens. This study was done to investigate the effect of Zataria multiflora with a quadruple Therapy on H-pylori eradication. Method: This study was performed on 60 patients referred to Kosar Hospital where H-pylori infection was detected by upper endoscopy and rapid urease test. Patients were divided into two groups: Group A received amoxicillin 1gr, metronidazole 500mg, Bismuth 250mg, omeprazole 20mg twice daily and Zataria multiflora (5.5-6.5mg) three times daily. Group B (28 patients) were treated with the same diet without Zataria multiflora hc. Finally, H-pylori eradication was evaluated by the urea Breath test in both groups. Result: H-pylori eradication was observed in 86.4% (22 patients) of group A and 92.9% (26 patients) of group B and there was no significant difference between the two groups. Conclusion: The addition of Zataria multiflora was clearly not effective in eradicating H-pylori. However, the positive effects of this plant on the immune system and its indirect effects on reducing inflammation can be considered in future studies.
The potential of leaf extract of Pangium edule Reinw as HIV-1 protease inhibitor: A computational biology approach

Sefren Geiner Tumilaar1, Fatimawali Fatimawali1, Nurdjannah Jane Niode2, Yunus Effendi3, Rinaldi Idroes4, Ahmad Akroman Adam5, Ahmed Rakib6, Talha Bin Emran7, Trina Ekawati Tallei8* 1Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, Indonesia. 2Department of Dermatology and Venereology, Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia. 3Biology Study Program, Faculty of Science and Technology, Al Azhar University, Jakarta, Indonesia. 4Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, Indonesia. 5Dentistry Study Program, Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia. 6Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong, Bangladesh. 7Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh. 8Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado North Sulawesi, Indonesia.
On the Structure of Cities: Emergence of Residential and Industrial Areas under Environmental Policy

We study the internal structure of a city, in a model of economic geography, where industry and housing compete for scarce land to locate. We analyze a spatial model of a city in which a single good is produced using land, labor and emissions of a pollutant, and in which people consume goods, residential land and dislike pollution. The agglomeration e ects, caused by trade-o s between centripetal and centrifugal forces, in the form of knowledge spillovers, stringency of environmental policy and commuting cost, determine the emergence of industrial and residential clusters across space. JEL classi cation: R3, Q5, H2.
Generalized-ICP

—In this paper we combine the Iterative Closest Point (’ICP’) and ‘point-to-plane ICP‘ algorithms into a single probabilistic framework. We then use this framework to model locally planar surface structure from both scans instead of just the ”model” scan as is typically done with the point-to-plane method. This can be thought of as ‘plane-to-plane’. The new approach is tested with both simulated and real-world data and is shown to outperform both standard ICP and point-to-plane. Furthermore, the new approach is shown to be more robust to incorrect correspondences, and thus makes it easier to tune the maximum match distance parameter present in most variants of ICP. In addition to the demonstrated performance improvement, the proposed model allows for more expressive probabilistic models to be incorporated into the ICP framework. While maintaining the speed and simplicity of ICP, the Generalized-ICP could also allow for the addition of outlier terms, measurement noise, and other probabilistic techniques to increase robustness.
Canopy Based Aboveground Biomass and Carbon Stock Estimation of Wild Pistachio Trees in Arid Woodlands Using GeoEye-1 Images

Spatially explicit estimates of aboveground biomass over large area are necessary for natural resources managers. This study examined aboveground biomass and carbon stock of the wild pistachio (Pistacia atlantica) based on individual tree crown detection and allometric development in the arid woodlands using high-resolution satellite images of GeoEye-1 in a reserved forest area of Wild Pistachio trees in the South Khorasan Province, East of Iran. Biomass of sampled trees was determined using field sampling and experimental tests. In addition, the biomass of stems was determined using volume and density. The allometric biomass and carbon stock equations of Wild Pistachio trees were developed based on crown area, diameter at breast height (1.3 m), and height of trees. The trees crowns were detected and delineated on the GeoEye-1 images, using local maxima filters, and region growing segmentation algorithms, respectively. In addition, a morphological watershed transformation method was applied to split the connected and overlapped tree crowns. Performing algorithms was assessed using the measured field crown of sample trees by precision, recall, and overall accuracy indices. The biomass and carbon stock of trees of the study area were estimated using delineated crown area and the developed allometric equations. The results showed that the equation that used crown area could explain more than 80% of the remarked variation in biomass and carbon stock. In addition, the crown detection method results showed that overall detection rate and the quality of crown boundaries were acceptable. In conclusion, the study confirmed that combining the allometric equations with crown information from high-resolution images could contribute to the explicit mapping of biomass and carbon stock of wild pistachio trees in the arid woodlands.
58-65 Chua TH.pmd

An outbreak of dengue in Kudat, northern Sabah in 2016-2017 provided an opportunity to investigate the circulating serotypes of dengue viruses of cases at Hospital Kudat. Between September 2016 and December 2017, a total of 156 dengue positive sera (tested positive by either NS1 antigen, or IgM & IgG antibody rapid test) were collected from dengue patients who had acute fever and showed signs and symptoms suggestive of dengue. RNA was extracted from the sera using QIAamp RNA Blood Mini Kit, and molecular amplification was performed using one-step RT-PCR kit, followed by nested PCR using HotStart Taq master mix kit with the primers of the dengue C-prM gene. There were 81 (52%) male and 75 (48%) female cases. The age group with the highest number of cases was the 10-19 years old, while the youngest infected was 8 months old and the oldest was 83 years old. RT-PCR results showed 88 sera dengue positive, 48 infected with a single serotype while another 40 with multiple serotypes. All four DENV serotypes were co-circulating during the outbreak period and DENV-1 was predominant. Molecular analysis also indicated 69.2%, 50.0%, 51.9% and 48.9% respectively of the NS1, IgM, IgG and IgM & IgG positive sera were RT-PCR positive for dengue. High number of cases were seen in December 2016, February and May 2017. The dengue outbreak might be related to switching of predominant serotype from DENV 4 to DENV 1.
Response of some maize hybrids (Zea mays L.) for foliar application by polyisoprenic on bioactive components grown under middle Egyptian conditions

Grains of three maize hybrids grown in the middle Egypt zone were obtained and investigated for study the effect of spraying treatment with two β-carotene (βC) levels and natural carotenoids extract (NCE). The maize hybrids are yellow hybrid (No. 352) Yellow hybrid (No.178) and High Tec. White (No.2031). Results indicate that the most efficient solvent for carotenoids extraction is boiled methanol whereas the extract weight reached to be 0.913 and 0.9692 g/250 g dry weight grains followed by chloroform and ethanol. Application results of βC and NCE on crude lipids show that yellow hybrid (No. 352) recorded the highest levels followed by yellow hybrid (No. 178) and the lowest levels are recorded in High Tec. White (No. 2130). The concentrations with 7.5 and 15 μg/mL βC are better than NCE. It could be concluded that response of lipids in maize hybrids for foliar application are low. The yellow corn genotypes studied here are characterized with high levels of different isoforms of carotenes and xanthophylls and the results show that the most predominant carotene is β-carotene and the three predominant xanthophyll are zeaxanthin folowed by lutein and βcryptoxanthin. Total carotenoids in untreated and treated samples ranged from 47.63 to 568.49 μg /g and the highest concentration was recorded with 15 ppm β-carotene as foliar spraying. The Yellow hybrid (No.178) contains higher concentrations from lutein, zeaxanthin, b-cryptoxanthin and b-caotene compared with Yellow hybrid (No.352) and High Tec. White (No.2031). Generally, provitamin A carotenoid constitute 7.65-39.34 % of total carotenoids in maize, whereas zeaxanthin and lutein each commonly represent 30–50 %. Results show that the provitamin A carotenoid compose one third and xanthophyll carotenoids reached to be two thirds. The amounts of provitamin A in traditional yellow maize varieties range from 0.25 μg to 2.5 μg gdry weight. Results obtained here, indicate that spraying with polyisoprenic led to the accumulation of xanthophylls with 60% and carotenes with 40%. The precursors of vitamin A are less and this is due to the genotypes behavior under study which directed to the accumulation of both xanthophylls and carotenes, but the xanthophylls were predominant and higher.
Preparation and characterization of a novel insulin-phospholipid complex

Purpose: The purpose of this study was to prepare and characterize an insulin-phospholipid complex. Methods: The complex was prepared by an anhydrous co-solvent lyophilization method. The physicochemical properties of the new complex including infrared spectrophotometry, X-ray diffractogram, liposolubility and apparent partition coeffi cient were determined. In addition, the hypoglycemic effects were evaluated in normal rats to determine if the production process for the phospholipid complex might destroy or change the bioactivity of insulin. Results: The results of infrared spectra showed that there was some interaction between insulin and phospholipid in the complex, but no new characteristic absorption peaks were observed, indicating that no new covalent bonds were formed. The X-ray diffraction patterns suggested that insulin in the phospholipid complex was either molecularly dispersed or in an amorphous form. The liposolubility in the hydrophobic organic solvent, dichloromethane, demonstrated the improved lipophilicity of insulin in the phospholipid complex. The apparent partition coeffi cient of insulin complexed with phospholipid was related to the ionic strength and pH of the aqueous dispersion medium. The low ionic strength and pH close to the isoelectric point of insulin (pI 5.3) will increase the binding stability of the complex. Moreover, the bioactivity was maintained when insulin was complexed with phospholipid, and the production process of the complex did not change or destroy the molecular structure of the insulin inside the complex. Conclusion: Compared with native insulin, the physicochemical properties of insulin changed signifi cantly after it was complexed with phospholipid. These characteristics, especially the improved lipophilicity, will contribute to the improved oral absorption of insulin.
The Effect of Seaweed Extracts on Chemical Composition of Tomato Plant (Solanum lycopersicum)

Seaweed extracts usually used in agriculture and horticulture as nutrient supplements, biostimulants, or biofertilizers to improve plant growth and yield. In this study, to explain these effects. GC-MS was used to measure the change in chemical composition of tomato plant that treated with liquid seaweed extracts (LSWE). The effect of LSWE isolated from Ulva lactuca (green algae) and Grateloupia filicina (red algae), on the chemical composition of tomato (Solanum lycopersicum) that ground under greenhouse condition. Were assessed LSWE was sprayed at concentration (1.5 %) on tomato seedlings seven times (one a week) at the end of seven weeks tomato leaves were collected from each treatment then extracted and subjected to GC-MS analysis. Our results indicate that seedlings treated with LSWE increased the percentage of terpenes in plant leaf extract compared to untreated plants that contained (17.15%), while in plants treated with Ulva lactuca and Grateloupia filicina extract it was (35.52%) and (35.87%) respectively. The terpenes content monoterpenes and sesquiterpenes. No pronounced differences were estimated in the aliphatic alcohols content between the treated and untreated plants. Moreover, treatment with Ulva lactuca extract was shown to have an effect in increasing the level of fatty acids 18% in tomato plant leaves, while there was no effect of treatment with Grateloupia filicina extract, (11%) compared with untreated plants (12%).
User Behavior of Online Public Complaint and Aspiration Service (LAPOR!) in Mataram City

Public Complaint and Aspiration Service (LAPOR! ) was initially a program spearheaded by the Presidential Work Unit for Development Supervision and Control (UK4). This program is associated with the administration of public services by all government agencies. Furthermore, it is used by the Mataram City Government as a medium to facilitate public aspirations and complaints. Therefore, this research aims to comprehend the factors influencing the community in applying the LAPOR! Application in the Mataram city in 2019. This is a mixed method research with data obtained from 70 user communities through interviews with related agencies and by distributing questionnaires, with the PLS and SPSS applications used for analysis. The results showed that the variable performance expectancy, effort expectancy, and social influence significantly affect behavioral intention. Meanwhile, facilitating condition and behavioral intention has a positive and significant impact on the utilization of LAPOR! Application. These were indicated by the P-value <0.005 and the T statistical value> 1.96, thereby concluding that each hypothesis proposed in this study is accepted.
SIMULTANEOUS DETERMINATION OF CURCUMINOIDS IN CURCUMA SAMPLES USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

A rapid and simple high performance thin layer chromatographic method has been developed for the simultaneous quantitation of pharmacologically important diaryl heptanoids curcumin, demethoxy curcumin, and bis-demethoxy curcumin in Curcuma longa and C. amada. The assay combines the isolation and separation of curcuminoids on silica gel 60F254 high performance thin layer chromatographic plates, followed by scanning of the spots at 366 nm using a UV detection mode.