Miracle-dz/UniFAR-FTUs huggingface dataset:
Production and Immunological Characterization of scFv Specific to Epitope of Opisthorchis viverrini Rhophilin-Associated Tail Protein 1-like (OvROPN1L)

(1) Background: Opisthorchis viverrini is a significant health problem in the Mekong subregion of Southeast Asia, causing aggressive cholangiocarcinoma. Current diagnostic procedures do not cover early diagnosis and low infection. Hence, an effective diagnostic tool is still required. Immunodiagnosis seems promising, but attempts to generate monoclonal antibodies have not yet been successful. This study aims to develop a single-chain variable antibody fragment (scFv) against Rhophilin-associated tail protein 1-like (ROPN1L), the sperm-specific antigen of adult O. viverrini, which has not been reported elsewhere. (2) Methods: The target epitope for phage screening was L3-Q13 of OvROPN1L, which showed the highest antigenicity to human opisthorchiasis analyzed in a previous study. This peptide was commercially synthesized and used for phage library screening. The isolated phage was produced in a bacterial expression system and tested for specificity in vitro and in silico. (3) Results: One of fourteen phages, named scFv anti-OvROPN1L-CL19, significantly bound to rOvROPN1L compared with non-infected hamster fecal extracts. This phage clone was successfully produced and purified using Ni-NTA chromatography. Indirect ELISA demonstrated that scFv anti-OvROPN1L-CL19 has a high reactivity with O. viverrini-infected hamster fecal extracts (12 wpi, n = 6) in comparison with non-infected hamster fecal extracts (0 wpi, n = 6), while the polyclonal rOvROPN1L antibodies did not show such a difference. Molecular modeling and docking confirmed our in vitro findings. (4) Conclusion: scFv anti-OvROPN1L-CL19 could be used as an effective material for developing O. viverrini-immunodiagnostic procedures in the future.
Mechanical and thermal transport properties of graphene with defects

The roles of defects including monatomic vacancies and Stone-Wales dislocations in the mechanical and thermal properties of graphene are investigated here through molecular dynamics (MD) simulations. The results show that Young’s modulus of a defected graphene sheet has a gentle dependence with the concentration of defects, while the thermal conductivity is much more sensitive. Analysis based on the effective medium theory (EMT) indicates that this sensitivity originates from the scattering of phonons by defects and delocalized interaction between them, which leads to a transition from propagating to diffusive mode as the concentration increases.
Nonstationary behavior of the gyrotron backward-wave oscillator

Nonstationary processes in the gyrotron backward-wave oscillator (gyro-BWO) are investigated numerically using a time-dependent self-consistent model which allows the authors to study multifrequency operation of the device. The results indicate that the behavior of the gyro-BWO is strongly influenced by higher order axial modes (HOMs) whose excitation results from an increase in gyro-BWO beam current or structure length. In both cases, the gyro-BWO behavior changes from stationary to nonstationary, however, in different ways. Furthermore, the influence of HOM's excitation on the gyro-BWO efficiency and its output signal spectrum is analyzed. Moreover, a comparative study of two regimes of operation-the gyro-BWO and the gyromonotron-is conducted and the obtained results are compared to experiments.
Cannabimimetic N-Stearoylethanolamine as “Double-Edged Sword” in Anticancer Chemotherapy: Proapoptotic Effect on Tumor Cells and Suppression of Tumor Growth versus Its Bio-Protective Actions in Complex with Polymeric Carrier on General Toxicity of Doxorubicin In Vivo

This study reports a dose-dependent pro-apoptotic action of synthetic cannabimimetic N-stearoylethanolamine (NSE) on diverse cancer cell lines, including multidrug-resistant models. No antioxidant or cytoprotective effects of NSE were found when it was applied together with doxorubicin. A complex of NSE with the polymeric carrier poly(5-(tert-butylperoxy)-5-methyl-1-hexen-3-yn-co-glycidyl methacrylate)-graft-PEG was synthesized. Co-immobilization of NSE and doxorubicin on this carrier led to a 2-10-fold enhancement of the anticancer activity, particularly, against drug-resistant cells overexpressing ABCC1 and ABCB1. This effect might be caused by accelerated nuclear accumulation of doxorubicin in cancer cells, which led to the activation of the caspase cascade, revealed by Western blot analysis. The NSE-containing polymeric carrier was also able to significantly enhance the therapeutic activity of doxorubicin in mice with implanted NK/Ly lymphoma or L1210 leukemia, leading to the complete eradication of these malignancies. Simultaneously, loading to the carrier prevented doxorubicin-induced elevation of AST and ALT as well as leukopenia in healthy Balb/c mice. Thus, a unique bi-functionality of the novel pharmaceutical formulation of NSE was revealed. It enhanced doxorubicin-induced apoptosis in cancer cells in vitro and promoted its anticancer activity against lymphoma and leukemia models in vivo. Simultaneously, it was very well tolerated preventing frequently observed doxorubicin-associated adverse effects.
Regularity criteria for the incompressible magnetohydrodynamic equations with partial viscosity

We prove some regularity criteria for the incompressible magnetohydrodynamic equations with partial viscosity. We study three cases: incompressible magnetohydrodynamic equations with zero viscosity in a bounded domain, incompressible magnetohydrodynamic equations with zero resistivity in a bounded domain, and the density-dependent magnetohydrodynamic equations with zero heat conductivity and zero resistivity in the whole space ℝ3. Our results extend and improve some known results.
The modified mild-slope equation

A modified version of the mild-slope equation is derived and its predictions of wave scattering by two-dimensional topography compared with those of other equations and with experimental data. In particular, the modified mild-slope equation is shown to be capable of describing known scattering properties of singly and doubly periodic ripple beds, for which the mild-slope equation fails. The new equation compares favourably with other models of scattering which improve on the mild-slope equation, in that it is widely applicable and computationally cheap.
Dispersion on a sphere

Any topological framework requires the development of a theory of errors of characteristic and appropriate mathematical form. The paper develops a form of theory which appears to be appropriate to measurements of position on a sphere. The primary problems of estimation as applied to the true direction, and the precision of observations, are discussed in the subcases which arise. The simultaneous distribution of the amplitude and direction of the vector sum of a number of random unit vectors of given precision, is demonstrated. From this is derived the test of significance appropriate to a worker whose knowledge of precision lies entirely in the internal evidence of the sample. This is the analogue of ‘Student’s’ test in the Gaussian theory of errors. The general formulae obtained are illustrated using measurements of the direction of remanent magnetization in the directly and inversely magnetized lava flows obtained in Iceland by Mr J. Hospers.
The Profile of Palaeontology Teaching in Undergraduate Courses in Brazil

Background: Currently, palaeontology professors have been challenged to update and incorporate innovative teaching approaches into their planning to help students develop the necessary skills and competencies. However, today there is a mismatch between the current and the ideal teaching profiles. Objective: To describe the palaeontology teaching profile in undergraduate courses in Brazil. Design: Descriptive and exploratory study. Setting and participants: Palaeontology professors (n=37) in undergraduate courses at several Brazilian higher education institutions. Data analysis collection: Data were collected through a semi-structured online form (survey), triangulated and categorised into (a) general characteristics, (b) teacher education, (c) teaching experience, (d) undergraduate courses, (e) curriculum, syllabus, and programme discussion meetings, (f) inter and multidisciplinarity, and (g) curriculum. Results: Despite the limited scope (small sample size), the study results guided the focus of the research and improvement efforts, as there are still many difficulties and challenges to be overcome to raise the quality of teaching in undergraduate palaeontology across the country. Conclusions: The data from this study can serve as a valuable basis for professors-researchers, educational managers, and policymakers interested in implementing innovative continuing education programmes and in supporting management and policy decisions regarding potential reforms of palaeontology teaching in Brazilian undergraduate courses in higher education institutions.
Initiation of Detonations

In order to study the initiation of detonation waves, the time dependent one‐dimensional hydrodynamic equations have been numerically integrated for several initial conditions. In all the cases treated, the hydrodynamic motion and the release of chemical energy are practically independent as a consequence of the extreme temperature sensitivity of the reaction rate. At first the chemical energy is released so slowly that it has no effect on the hydrodynamic motion. Thereafter the reaction proceeds so rapidly that the burning is not influenced by the hydrodynamic motion. A simple criterion is established to determine whether or not detonation will result from given initial conditions of pressure and temperature. This criterion is applied to determine how the detonability depends upon the duration of the initial pressure pulse and upon the initial temperature distribution.
THE SENDOV CONJECTURE FOR POLYNOMIALS WITH AT MOST SEVEN DISTINCT ZEROS

We prove that Sendov's conjecture is true for polynomials of degree N = 7 and determine the so-called extremal polynomials in this case. As a consequence of a general result concerning polynomials with a prescribed number of distinct zeros (Theorem 2.4), we also check the validity of the conjecture for polynomials with at most seven distinct roots. Some properties of the extremal polynomials are discussed as well. 1991 MATH. SUBJ. CLASS. : Primary 30C15; Secondary 30C10
Prediction of Relevant Training Control Parameters at Individual Anaerobic Threshold without Blood Lactate Measurement

Background: Active exercise therapy plays an essential role in tackling the global burden of obesity. Optimizing recommendations in individual training therapy requires that the essential parameters heart rate HR(IAT) and work load (W/kg(IAT) at individual anaerobic threshold (IAT) are known. Performance diagnostics with blood lactate is one of the most established methods for these kinds of diagnostics, yet it is also time consuming and expensive. Methods: To establish a regression model which allows HR(IAT) and (W/kg(IAT) to be predicted without measuring blood lactate, a total of 1234 performance protocols with blood lactate in cycle ergometry were analyzed. Multiple linear regression analyses were performed to predict the essential parameters (HR(IAT)) (W/kg(IAT)) by using routine parameters for ergometry without blood lactate. Results: HR(IAT) can be predicted with an RMSE of 8.77 bpm (p < 0.001), R2 = 0.799 (|R| = 0.798) without performing blood lactate diagnostics during cycle ergometry. In addition, it is possible to predict W/kg(IAT) with an RMSE (root mean square error) of 0.241 W/kg (p < 0.001), R2 = 0.897 (|R| = 0.897). Conclusions: It is possible to predict essential parameters for training management without measuring blood lactate. This model can easily be used in preventive medicine and results in an inexpensive yet better training management of the general population, which is essential for public health.
Automatic adaptive emotion regulation is associated with lower emotion-related activation in the frontoparietal cortex and other cortical regions with multi-componential organization

Although some researchers consider automatic adaptive emotion regulation to be an automatized strategy whereas others consider it to be implicit disengagement of deliberative process, to date, its neural correlates have been poorly investigated. In addition, the valence specificity of automatic adaptive emotion regulation and levels of activation relative to the neutral condition are controversial; the former is relevant to the attribution of resilient emotion regulation to positivity bias or emotional stability, and the latter to determining whether regulation is based on emotion-specific or emotion-non-specific processes. In this functional magnetic resonance imaging (fMRI) study, we presented positive and negative emotional pictures to healthy young participants and investigated the neural correlates of automatic adaptive emotion regulation in spontaneous emotional response. A significant negative trait effect (i.e., regression coefficient) on activation was identified both for positive and negative emotional responses in various cortical regions. A cluster analysis identified three clusters among these regions based on the valence specificity of the trait effect and level of activation relative to neutral stimuli. Cluster 1 included regions in the sensorimotor cortex characterized by negative emotion-specific decreases in activation relative to neutral stimuli in adaptive individuals. Cluster 2 included several cortical regions including the bilateral dorsal executive network, anterior cingulate, and inferior frontal gyrus, which were characterized by valence-independent decreases in activation in adaptive individuals. Cluster 3 included the bilateral ventrolateral and dorsomedial prefrontal cortices, right insula, and other posterior regions, which were characterized by increased activation for negative stimuli in non-adaptive individuals. These findings support the assumption that automatic adaptive emotion regulation involves the implicit disengagement of deliberative process and suggest the relevance of different cortical networks to the potential emotion- and valence-specificity of adaptive regulation.
Application of Genetic Algorithm to the Position Optimization of the Outriggers for Trimaran

In multi-hulls ship development the position optimization of the outriggers for Trimaran has to be taken into account. This problem may be solved by multi-objects nonlinear program. In this paper, The Rankine Source method based on the potential flow theory is used to predict the wave-making resistance of the trimaran and the Multi-Objective Genetic Algorithms (MOGAs) is used to optimize the position of the outriggers and the optimum results of CFD method were given too. The results show that MOGAs is an efficient and qualified engineering optimum design method in ship design.
Evaluation of Electrochemotherapy with Bleomycin in the Treatment of Colorectal Hepatic Metastases in a Rat Model

Simple Summary The available ablative procedures for the treatment of hepatic cancer have contraindications due to the heat-sink effect and the risk of thermal injuries. Electrochemotherapy (ECT) represents an ablative procedure that combines the administration of chemotherapeutic agents with well-dosed electric pulses for cell membrane reversible electroporation (rEP). In ECT, the enhanced cellular permeability facilitates the transportation of chemotherapeutic agents into tumor cells. In our study, ECT was compared with rEP and chemotherapy in a rat liver metastasis model. The ECT group showed a stronger reduction in tumor oxygenation compared to the rEP and chemotherapy groups. Histological analyses revealed a significantly increased tumor necrosis of >85% and a reduced tumor vascularization in the ECT group. Consequently, ECT is an effective treatment of hepatic tumors and may be utilized for the treatment of tumors adjacent to high-risk regions, where other ablative procedures are contraindicated. Abstract Background: The available ablative procedures for the treatment of hepatic cancer have contraindications due to the heat-sink effect and the risk of thermal injuries. Electrochemotherapy (ECT) as a nonthermal approach may be utilized for the treatment of tumors adjacent to high-risk regions. We evaluated the effectiveness of ECT in a rat model. Methods: WAG/Rij rats were randomized to four groups and underwent ECT, reversible electroporation (rEP), or intravenous injection of bleomycin (BLM) eight days after subcapsular hepatic tumor implantation. The fourth group served as Sham. Tumor volume and oxygenation were measured before and five days after the treatment using ultrasound and photoacoustic imaging; thereafter, liver and tumor tissue were additionally analysed by histology and immunohistochemistry. Results: The ECT group showed a stronger reduction in tumor oxygenation compared to the rEP and BLM groups; moreover, ECT-treated tumors exhibited the lowest levels of hemoglobin concentration compared to the other groups. Histological analyses further revealed a significantly increased tumor necrosis of >85% and a reduced tumor vascularization in the ECT group compared to the rEP, BLM, and Sham groups. Conclusion: ECT is an effective approach for the treatment of hepatic tumors with necrosis rates >85% five days following treatment.
CFD Simulations of Ventilation and Interunit Dispersion in Dormitory Complex: A Case Study of Epidemic Outbreak in Shanghai

Since the beginning of March 2022, a new round of COVID-19 outbreaks in Shanghai has led to a sharp increase in the number of infected people. It is important to identify possible pollutant transmission routes and predict potential infection risks for infectious diseases. Therefore, this study investigated the cross-diffusion of pollutants caused by natural ventilation, including external windows and indoor ventilation windows, under three wind directions in a densely populated building environment with the CFD method. In this study, CFD building models were developed based on an actual dormitory complex and surrounding buildings under realistic wind conditions to reproduce the airflow fields and transmission paths of pollutants. This paper adopted the Wells–Riley model to assess the risk of cross-infection. The biggest risk of infection was when a source room was located on the windward side, and the risk of infection in other rooms on the same side as the source room was large in the windward direction. When pollutants were released from room 8, north wind resulted in the highest concentration of pollutants in room 28, reaching 37.8%. This paper summarizes the transmission risks related to the indoor and outdoor environments of compact buildings.
Procedures for the identification of multiple influential observations in linear regression

Since the seminal paper by Cook (1977) in which he introduced Cook's distance, the identification of influential observations has received a great deal of interest and extensive investigation in linear regression. It is well documented that most of the popular diagnostic measures that are based on single-case deletion can mislead the analysis in the presence of multiple influential observations because of the well-known masking and/or swamping phenomena. Atkinson (1981) proposed a modification of Cook's distance. In this paper we propose a further modification of the Cook's distance for the identification of a single influential observation. We then propose new measures for the identification of multiple influential observations, which are not affected by the masking and swamping problems. The efficiency of the new statistics is presented through several well-known data sets and a simulation study.
Utilizing laser interference lithography to fabricate hierarchical optical active nanostructures inspired by the blue Morpho butterfly

The famous non-iridescent blue of the Morpho butter by is caused by a `Christmas tree' like nanostructure which is a challenge for common fabrication techniques. Here, we introduce a method to fabricate this complex morphology utilizing dual beam interference lithography. We add a reflective coating below the photoresist to create a second interference pattern in vertical direction by exploiting the back reflection from the substrate. This vertical pattern exposes the lamella structure into the photosensitive polymer while the horizontal interference pattern determines the distance of the ridges. The photosensitive polymer is chosen accordingly to create the Christmas tree' like tapered shape. The resulting artificial Morpho replica shows brilliant non-iridescent blue up to an incident angle of 40. Its optical properties are close to the original Morpho structure because the refractive index of the polymer is close to chitin. Moreover, the biomimetic surface is water repellent with a contact angle of 110.
On the Even Powers of the Antipode of a Finite-Dimensional Hopf Algebra

Abstract The trace of powers of the square of the antipode s 2 of a finite-dimensional Hopf algebra A over a field k is studied. It is shown in many cases that the trace function vanishes on s 2 m when s 2 m ≠ 1 A . Finer properties of the antipode are related to this phenomenon.
Substantial carbon sequestration by peatlands in temperate areas revealed by InSAR

Peatlands are unique ecosystems that contain massive amounts of carbon. These ecosystems are incredibly vulnerable to human disturbance and climate change. This may cause the peatland carbon sink to shift to a carbon source. A change in the carbon storage of peatlands may result in surface deformation. This research uses the interferometric synthetic aperture radar (InSAR) technique to measure the deformation of the peatland’s surface in south Sweden in response to the seasonal and extreme weather conditions in recent years, including the unprecedented severe drought in the summer of 2018. The deformation map of the study area is generated through a time-series analysis of InSAR from June 2017 to November 2020. Monitoring the peatland areas in this region is very important as agricultural and human activities have already caused many peatlands to disappear. This further emphasizes the importance of preserving the remaining peat sites in this region. Based on the InSAR results, a method for calculating the carbon flux of the peat areas is proposed, which can be utilized as a regular monitoring approach for other remote areas. Despite the severe drought in the summer of 2018, our findings reveal a significant uplift in most of the investigated peat areas during the study period. Based on our estimations, 86% of the peatlands in the study area experienced an uplift corresponding to about 47 000 tons of carbon uptake per year. In comparison, the remaining 14% showed either subsidence or stable conditions corresponding to about 2300 tons of carbon emission per year during the study period. This emphasizes the importance of InSAR as an efficient and accurate technique to monitor the deformation rate of peatlands, which have a vital role in the global carbon cycle.
China's current situation and development of hospice and palliative care in critical care medicine

COPYRIGHT © 2023 Su and Ning. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. China’s current situation and development of hospice and palliative care in critical care medicine