Miracle-dz/UniFAR-FTUs huggingface dataset:
hck Phosphorylation and Activation of p59 IL-2 Signaling in Human Monocytes Involves

The activating properties of IL-2 and the structure of the IL-2R on human monocytes are well characterized. However, relatively little is known about the biochemical mechanisms involved in IL-2 signal transduction in these cells. We investigated the role of protein tyrosine kinases (PTKs) in the activation of monocytes by IL-2. Incubation of monocytes with the PTK inhibitor herbimycin A (HA) resulted in the dose-dependent suppression of IL-2-induced monocyte tumoricidal activity. This inhibition was rather potent, as a concentration of HA as low as 0.5 m M caused a complete abrogation of cytolytic activity. Furthermore, HA markedly suppressed the ability of IL-2 to induce IL-1 b , TNF- a , IL-6, and IL-8 mRNA expression and protein secretion by monocytes. Anti-phosphotyrosine immunoblotting demonstrated that IL-2 induced a rapid and time-dependent increase in tyrosine phosphorylation of several cellular proteins of molecular masses ranging from 35 to 180 kDa. Interestingly, IL-2 caused a signiﬁcant up-regulation of the constitutive levels of hck PTK mRNA and protein relative to medium-treated cells as well as an increase in p59 hck tyrosine phosphorylation. Finally, we demonstrated by in vitro kinase assay that the speciﬁc activity of p59 hck PTK was also induced by IL-2 in monocytes. Thus, these data show that the activation of PTKs is required for the triggering of monocyte effector and secretory functions by IL-2 and strongly suggest that p59 hck is a key participant in IL-2 signaling in human monocytes. The Journal of Immunology, 2000, 164: 4575–4585.
Sensitivity of Atmospheric River Vapor Transport and Precipitation to Uniform Sea Surface Temperature Increases

Filaments of intense vapor transport called atmospheric rivers (ARs) are responsible for the majority of poleward vapor transport in the midlatitudes. Despite their importance to the hydrologic cycle, there remain many unanswered questions about changes to ARs in a warming climate. In this study we perform a series of escalating uniform SST increases (+2, +4, and +6K, respectively) in the Community Atmosphere Model version 5 in an aquaplanet configuration to evaluate the thermodynamic and dynamical response of AR vapor content, transport, and precipitation to warming SSTs. We find that AR column integrated water vapor (IWV) is especially sensitive to SST and increases by 6.3–9.7% per degree warming despite decreasing relative humidity through much of the column. Further analysis provides a more nuanced view of AR IWV changes: Since SST warming is modest compared to that in the midtroposphere, computing fractional changes in IWV with respect to SST results in finding spuriously large increases. Meanwhile, results here show that AR IWV transport increases relatively uniformly with temperature and at consistently lower rates than IWV, as modulated by systematically decreasing low‐level wind speeds. Similarly, changes in AR precipitation are related to a compensatory relationship between enhanced near‐surface moisture and damped vertical motions.
Genetic variability and character association studies in sesame (Sesamum indicum L.)

Genetic variability, character association and path analysis between yield and its contributing traits were studied in 28 sesame genotypes. Analysis of variance revealed the existence of significant differences among genotypes for all the characters studied. Seed yield per plot, capsules per plant and primary branches per plant showed high phenotypic and genotypic coefficients of variation coupled with high to moderate heritability and high genetic advance indicating the predominance of additive gene effects in controlling these traits. Seed yield per plot showed significantly positive correlations with all the traits studied. Path coefficient analysis indicated that plant height and capsules per plant were important traits to be considered for realizing improvement in seed yield.
Meningkatkan Hasil Belajar Biologi Melalui E-Modul Berbasis Problem Based Learning

Minimnya media inovatif dalam proses pembelajaran memberikan dampak yang signifikan pada penurunan hasil belajar siswa karena mereka merasa bosan dalam proses pembelajaran. Hal tersebut menjadi dasar yang mendorong dilaksanakannya penelitian ini. Penelitian ini bertujuan untuk mendeskripsikan proses bangun dan validitas E-modul berbasis PBL. Jenis penelitian ini adalah penelitian pengembangan dengan model ADDIE yang terdiri dari lima tahapan yaitu analisis, desain, pengembangan, implementasi dan evaluasi. Metode yang digunakan untuk pengumpulan data yaitu observasi, wawancara, dan kuesioner. Teknik analisis data yang digunakan yaitu analisis deskriptif kualitatif dan analisis deskriptif kuantitatif. Rancangan E-modul berbasis PBL valid dengan: (a) pembelajaran blended valid dengan: (a) hasil review ahli pelajaran dengan kualifikasi sangat baik (90,6%), (b) hasil review ahli desain pembelajaran dengan kualifikasi sangat baik (97,1%), (c) hasil review ahli media pembelajaran dengan kualifikasi sangat baik (92,5%), (d) hasil uji coba perorangan dengan kualifikasi sangat baik (95%), (e) hasil uji coba kelompok kecil dengan kualifikasi sangat baik (95%). Jadi, penelitian ini menunjukan bahwa validitas E-modul berbasis problem based learning pada kulifikasi sangat baik dan layak untuk digunakan dalam proses pembelajaran.
Building an OER program based on stakeholder feedback

Purpose This study aims to outline a library-led open educational resource (OER) training program for faculty and an assessment of barriers to OER adoption on campus. This study examines program assessment data (including faculty-reported needs to increase the likelihood of OER adoption) and analyzes a community-focused outreach strategy for a new OER program. Design/methodology/approach This program took a user-centered approach to developing campus support services for OER that specifically sought to address local needs and challenges. It intentionally incorporated strategies related to faculty motivation and satisfaction. Findings Although this faculty incentive program did not require OER adoption, a high number of voluntary OER adoptions occurred and participants showed interest in sharing information about OER across campus. Information about barriers to adoption informed future services. Practical implications This paper presents an adaptable model to launch new OER services and encourage a culture of using affordable course materials. Originality/value This project gathered information and identified collaborators to help build a sustainable, community-oriented OER program. The program focused early efforts on collecting and incorporating stakeholder feedback rather than moving directly to strategies focused on adopting or creating OER. It offers a model for other libraries to follow in creating sustainable practices.
Waiving the extinction debt: Can shade from coffee prevent extinctions of epiphytic plants from isolated trees?

Local extinction after habitat modifications is often delayed, leading to an extinction debt. Our first aim was to develop a conceptual model for natural and human‐mediated habitat improvements after a disturbance that may waive part of the predicted extinction debt. Second, we wanted to test this model on the distribution of epiphytic plants on trees that had been isolated in the agricultural matrix after forest clearing, around which coffee subsequently had been planted with a possible improvement of the microclimate.
Germ-Cell Culture Conditions Facilitate the Reprogramming to Produce Mouse Embryonic Stem Cells

SUMMARYThe derivation of embryonic stem-cell (ESC) lines from blastocysts is a very ineffi-cient process. Murine ESCs are thought to arise from epiblast cells that are alreadypredisposedtoaprimordial-germ-cellfate.DuringtheprocessofESCderivationfromB6D2F1hybridmice,ifwefirstculturetheembryofromthetwo-cellstageinmediumsupplementedwithLIF,weimprovethequalityoftheblastocyst.Whentheblastocystisthenculturedinagerm-linestem-cellculturemedium(GSCm),weareabletomoreefficiently (28.3%) obtain quality ESC lines that have a normal karyotype, properdegree of chimerism, and exhibit germ-line transmission when microinjected intoblastocysts.Althoughgerm-cell-specificgeneswereexpressedinallculturemediumconditions, GSCm did not shift the transcriptome towards germ-cell specification. Acorrelation wasfurther observed betweenESC derivation efficiencyand the expres-sion of some imprinted genes and retrotransposable elements. In conclusion, thecombinationofLIFsupplementationfollowedbycultureinGSCmestablishesahigherefficiency method for ESC derivation.Mol. Reprod. Dev. 2014. 2014 Wiley Periodicals, Inc.
Evidence for polyphosphate accumulating organism (PAO)-mediated phosphorus cycling in stream biofilms under alternating aerobic/anaerobic conditions

P availability often limits primary production in freshwater ecosystems and excessive P inputs promote accelerated eutrophication. Microbial mechanisms may control O2-dependent uptake/release of P in stream sediments and biofilms, but specific organisms responsible for these cycles have not been identified. Polyphosphate accumulating organisms (PAOs) are purposely enriched in treatment plants to remove P from wastewater. PAOs release P under anaerobic conditions and take it up under aerobic conditions. We hypothesized that alternating aerobic/anaerobic conditions promote patterns of P uptake/release similar to those attributed to PAOs in wastewater treatment. We collected intact, native stream biofilms and subjected them to laboratory treatments to impose conditions similar to what may occur because of diel oxygenic and respiratory cycles: 1) continuous sparging with air and 2) alternate sparging with air or anaerobic gas (20∶80% by volume CO2∶N2). We monitored PO43−, Ca, Mg, total Mn, K, Fe2+, and total S (TS) concentrations in the water during the experiment and total P (TP) and polyphosphate (polyP) concentrations in the biofilms at the start and end of the experiment. We used microscopy and polymerase chain reaction (PCR) to quantify the percentage of cells with stored intracellular polyP and to test for known PAO genes, respectively. The water had significantly greater dissolved PO43− concentrations during anaerobic than during aerobic conditions. Ca, K, Mg, and total Mn concentrations mimicked PO43− concentrations over time, but Fe2+ and TS concentrations did not. Precipitation of Ca and Mg and reductive dissolution of Mn may have influenced P cycling. Percent microbially stored intracellular polyP was nearly 3× greater in aerobic than anaerobic conditions. We did not find previously reported PAO genes in our biofilms, indicating the presence of novel polyP accumulators. Combined biotic and abiotic processes may be important in controlling short-term P cycling in stream biofilms.
DETERMINAÇÃO DE EQUAÇÃO VOLUMÉTRICA E HIPSOMÉTRICA PARA UM PLANTIO DE Tectona grandis L. F. EM ALTA FLORESTA - MT

O presente trabalho teve como objetivo ajustar e selecionar equacoes hipsometricas e volumetricas para um povoamento de Tectona grandis L. f. localizado na fazenda IDC, municipio de Alta Floresta - MT. A partir dos dados da altura e diâmetro e a cubagem rigorosa de 46 individuos selecionados, foi possivel ajustar os modelos hipsometricos e volumetricos. As equacoes foram selecionadas com base nos seguintes criterios: coeficiente de determinacao ajustado (R² aj ), erro padrao da estimativa (S yx %), e analise grafica dos residuos. Dentre os modelos testados, o modelo de Prodan, para relacao hipsometrica, e o de Schumacher-Hall, para relacao volumetrica, apresentaram melhores ajustes.
Maternal organophosphate flame‐retardant exposure alters offspring feeding, locomotor and exploratory behaviors in a sexually‐dimorphic manner in mice

Increased usage of organophosphate flame retardants (OPFRs) has led to detectable levels in pregnant women and neonates, which is associated with negative neurological outcomes. Therefore, we investigated if maternal OPFR exposure altered adult offspring feeding, locomotor, and anxiety‐like behaviors on a low‐fat (LFD) or high‐fat diet (HFD). Wild‐type C57Bl/6J dams were orally dosed with vehicle (sesame oil) or an OPFR mixture (1 mg/kg combination each of tris(1,3‐dichloro‐2‐propyl)phosphate, triphenyl phosphate and tricresyl phosphate) from gestation day 7 to postnatal day 14. After weaning, pups were fed either a LFD or HFD until 19 weeks of age. Locomotor and anxiety‐like behaviors were evaluated with the open field test, elevated plus maze, and metabolic cages. Feeding behaviors and meal patterns were analyzed by a Biological Data Acquisition System. Anogenital distance was reduced in OPFR‐exposed male pups, but no effect was detected on adult body weight. We observed interactions of OPFR exposure and HFD consumption on locomotor and anxiety‐like behavior in males, suggesting an anxiogenic effect while reducing overall nighttime activity. We also observed an interaction of OPFR exposure and HFD on weekly food intake and feeding behaviors. OPFR‐exposed males consumed more total HFD than oil‐exposed males during the 72‐hour trial. However, when arcuate gene expression was analyzed, OPFR exposure induced Agrp expression in females, which would suggest greater orexigenic tone. Collectively, the implications of our study are that the behavioral effects of OPFR exposure are modulated by adult HFD consumption, which may influence the metabolic and neurological consequences of maternal OPFR exposure.
SNP of cGH gene in Egyptian chicken breeds at MspI site

Genetic polymorphism of cGH gene using PCR-RFLP and identification of SNP among different genotypes of Dokki-4, Inshas, El-Salam and Mandarah breeds were studied. Amplification of GH gene (intron 4) for GH2 and GH3 loci produced 563 bp fragment length. The amplified fragments (563 bp) were digested with MspI endonuclease. Loci GH2 and GH3 showed two genotype frequencies TT and TC in all breeds, while genotype CC was absent in Dokki-4 of GH2 and absent in El-Salam of GH3. Analyzed data was compared with Genbank entries of Gallus gallus (KF957977), (KF957978), (KF957979), (KF957980) and (KF957981), the sequences of detected SNP were submitted to GenBank database with the accession numbers KY176737KY176758. Some Dokki-4 animals showed new two SNPs at positions T77C and C485T. Mandarah and Dokki-4 strains were shared in new three SNPs at positions G130A and C379T which was detected previously at KF957978. Mutation G430A of Mandarah and Dokki-4 was shown in KF957980. Eleven transitions mutations (five A/G and six C/T) and one transversion (G/C) were detected. Six transitions mutations C/T were silent mutations and position G358C was polymorphic in El-Salam.
A practical approach to wave energy modeling and control

The potential for control design to dramatically improve the economic viability of wave energy has generated a great deal of interest and excitement. However, for a number of reasons, the promised benefits from better control designs have yet to be widely realized by wave energy devices and wave energy remains a relatively nascent technology. This brief paper summarizes a simple, yet powerful approach to wave energy dynamics modeling, and subsequent control design based on impedance matching. Our approach leverages the same concepts that are exploited by a simple FM radio to achieve a feedback controller for wave energy devices that approaches optimal power absorption. If fully utilized, this approach can deliver immediate and consequential reductions to the cost of wave energy. Additionally, this approach provides the necessary framework for control co-design of a WEC, in which an understanding of the control logic allows for synchronous design of the device control system and hardware.
Coronavirus Disease-19 and Liver Injury

Coronavirus Disease (COVID)-19 is a pandemic since March 11, 2020. The total case is more than a half million worldwide. Liver injury is quite common in COVID-19 patients. Direct viral infection is possible due to the presence of angiotensin converting enzyme 2 in cholangiocytes and hepatocytes. Other proposed mechanisms are virus-induced cytopathic effects, inflammation process, hypoxia and shock, increased apoptotic activity, increased positive end expiratory effect, and drug-induced. The manifestation of liver injury is mild and transient with elevated liver enzymes, bilirubin, and gamma-glutamyl transferase levels. Deterioration of liver function can occur in subjects with COVID-19 and underlying liver injury. The management is principally supportive. Hepatoprotective drugs may be administered in severe cases.
Using Pseudomonas fluorescens and Bromoxynil herbicide for controlling pests and improving growth and productivity of two faba bean cultivars

Abstract Pseudomonas fluorescens and Bromoxynil herbicide, alone or in combination, were applied in the field naturally infected with Rhizoctonia solani (root rot disease), Meloidogyne arenaria (root-knot nematode) and different weed species for improved growth and productivity in two faba bean cultivars. All treatments reduced the root rot incidence in the range of 33 to 82%, compared to the untreated control and application of P. fluorescens alone had the best inhibitory effect. Combined P. fluorescens with the herbicide reduced the nematode parameters (i.e. J2 in soil and J2, females, eggs, galls & egg-masses in roots) as well as the weed growth parameters, compared to individual treatments or untreated controls. P. fluorescens alone or in combination with Bromoxynil increased faba bean growth and yield parameters. Most enhancement effects on growth and yield of faba bean achieved by P. fluorescens alone treatment. Total microbial and Rhizobium nodules counts were increased as affected by microbial treatments.
Porcine epidemic diarrhea virus in the United States: Cell culture isolation, genetic phylogeny, pathogenesis, and immunity

65 Introduction . 66 Materials and Methods . 68 Clinical samples . 68 PEDV N gene-based real-time RT-PCR . 69 Virus isolation, propagation, and titration . 69 Immunofluorescence assay . 70 EM . 71 NGS . 71 Sanger sequencing . 72 Phylogenetic analysis . 73 Nucleotide sequence accession numbers . 74
Analysis of Supply Chain Management Affecting the Underpricing of Companies Conducting Initial Public Offerings (IPO) on Indonesia Stock Exchange in 2018

Abstract- In the initial public offering activities of the company to obtain funds from the public, there is often an underpricing phenomenon, which is when the determination of the company's stock price at the time of an IPO is lower than the price that occurs on the secondary market on the first day. This study aims to analyze the influence of supply chain management (underwriter reputation, supply chain management reputation, firm age, and percentage of the public offering to underpricing of companies conducting IPO on the Indonesia Stock Exchange in 2018). Research data were cross-section data with observation period from 2nd January to 31st December 2018. The sampling method used was purposive sampling. From the population of 57 companies conducting IPOs, 54 issuers met the sample criteria. The analytical method used in this study was multiple linear regression. The results showed that the reputation of the underwriter, supply chain management's reputation, firm age, and percentage of public offering simultaneously influenced underpricing. Partially, the reputation of the underwriter and supply chain management's reputation have a significant negative effect, while the firm age and the percentage of the public offering do not have a significant effect. Underwriter reputation has the biggest influence on underpricing.
The Transition to Parenthood in the French and German Speaking Parts of Switzerland

After the first transition to parenthood, most couples adopt a gendered labor division, where mothers become main caregivers and fathers breadwinners of the family. By comparing two distinct language regions within one country, the present article explores how parents’ gendered labor division comes into existence and what role gendered culture and social policy play. The analysis draws on in-depth interviews with 23 German speaking and 73 French speaking participants from Switzerland. The results reveal that French speaking women and men presume an egalitarian labor division as parents. In German speaking regions, however, participants anticipate that mothers will become the main caregivers and fathers the breadwinners. It is shown that the labor market structure, which is in line with the male breadwinner norm, contributes to men’s full-time employment, whereas mothers’ labor market insertion is influenced by the acceptance of non-parental childcare and to a lesser extent by the offer of childcare facilities. Further, mothers experience more time conflicts than fathers, and the less mothers’ paid work is accepted, the more they suffer from feelings of guilt when being employed.
Examining Feminist and Critical Consciousness Conceptualizations of Women’s Subjective Well-Beingψ

Feminist and critical consciousness theories, that shaped the practice of feminist therapy, are unique in considering the impact of power and privilege on women’s well-being. We investigated tenets of these conceptualizations cross-sectionally by examining relations of critical consciousness, feminist collective action, personal empowerment, and subjective well-being among 247 women recruited via MTurk. We found that critical consciousness had a significant, positive direct link with collective action. Collective action, in turn, had significant, positive direct links with life satisfaction and positive affect. Importantly, critical consciousness via collective action was associated indirectly with greater satisfaction and positive affect. In contrast, critical consciousness was associated directly with lower satisfaction and positive affect. Personal empowerment was associated directly with greater well-being, but only collective action was a mechanism through which critical consciousness was associated with greater well-being. These findings are consistent with theory and support connecting the personal with the political in feminist therapy.
Rapid and Nondestructive Determination of Egg Freshness Category and Marked Date of Lay using Spectral Fingerprint

The potential of nondestructive prediction of egg freshness based on near-infrared (NIR) spectra fingerprints would be beneficial to quality control officers and consumers alike. In this study, handheld NIR spectrometer in the range of 740 nm to 1070 nm and chemometrics were used to simultaneously determine egg freshness based on marked date of lay for eggs stored under cold and ambient conditions. The spectra acquired from the eggs were preprocessed using multiplicative scatter correction and principal component analysis (MSC-PCA). Linear discriminant analysis (LDA) was used to build identification model to predict the category of freshness, while partial least square regression (PLS-R) was used to determine the marked date of lay. The performance of LDA model was above 95% identification rate in both calibration and prediction set for the eggs stored under ambient and cold storage. For eggs stored in ambient storage, LDA had 95.54% identification rate at 5 principal components, while at cold storage LDA has 100% identification rate at 5 principal components for determining the marked date of lay, and partial least square regression (PLS-R) gave R = 0.87 and RMSEI = 2.57 for ambient storage and R = 0.88 and RMSEI = 2.66 for cold storage in independent set, respectively. The results show that handheld spectrometer and multivariate analysis could be used for rapid and nondestructive measurement of egg freshness. This provides a novel solution for egg integrity prediction along the value chain.