Miracle-dz/UniFAR-FTUs huggingface dataset:
The role of ageing in the wish to be dead: disentangling age, period and cohort effects in suicide ideation in European population

Abstract Aims To investigate potential age, period and birth cohort effects in the prevalence of suicide ideation in European ageing population. Methods A total of 50 782 community-dwelling adults (aged + 50) from 20 different European countries were collected in the Survey Health Ageing and Retirement study. A multilevel logistic regression model of repeated measures was modelled to assess the effects of age and other variables, including the variability of observations over three levels: birth cohort groups, time period assessment and individual differences. Results The larger effect of variability was attributed to individual-level factors (57.8%). Youngest-old people (65–79 years) showed lower suicide ideation than middle-aged people (50–64 years). No significative differences were found for suicide ideation between middle-aged people and oldest-old (80 + years). Only 0.85% and 0.13% of the total variability of suicide ideation accounted for birth cohort and period effects, respectively. Cohorts born between 1941 and 1944 possessed the lowest estimates of suicide ideation. Conversely, suicide ideation started to rise with post-War generations and reached a significant level for people born from 1953–1957 to 1961–1964. Regarding the time period, participants assessed in 2006–2007 showed a lower likelihood of suicide ideation. The rest of the cohorts and period groups did not show any significant effect on the prevalence of suicide ideation. Conclusions Our results suggest that age and suicide ideation relationship is not linear in middle and older age. The European Baby boomers born from 50s to mid-60s might report higher suicide ideation than their ancestors. This scenario would imply a greater need for mental healthcare services for older people in the future.
Mapping of SARS-CoV-2 Brain Invasion and Histopathology in COVID-19 Disease

The coronavirus SARS-CoV-2 (SCV2) causes acute respiratory distress, termed COVID-19 disease, with substantial morbidity and mortality. As SCV2 is related to previously-studied coronaviruses that have been shown to have the capability for brain invasion, it seems likely that SCV2 may be able to do so as well. To date, although there have been many clinical and autopsy-based reports that describe a broad range of SCV2-associated neurological conditions, it is unclear what fraction of these have been due to direct CNS invasion versus indirect effects caused by systemic reactions to critical illness. Still critically lacking is a comprehensive tissue-based survey of the CNS presence and specific neuropathology of SCV2 in humans. We conducted an extensive neuroanatomical survey of RT-PCR-detected SCV2 in 16 brain regions from 20 subjects who died of COVID-19 disease. Targeted areas were those with cranial nerve nuclei, including the olfactory bulb, medullary dorsal motor nucleus of the vagus nerve and the pontine trigeminal nerve nuclei, as well as areas possibly exposed to hematogenous entry, including the choroid plexus, leptomeninges, median eminence of the hypothalamus and area postrema of the medulla. Subjects ranged in age from 38 to 97 (mean 77) with 9 females and 11 males. Most subjects had typical age-related neuropathological findings. Two subjects had severe neuropathology, one with a large acute cerebral infarction and one with hemorrhagic encephalitis, that was unequivocally related to their COVID-19 disease while most of the 18 other subjects had non-specific histopathology including focal B-amyloid precursor protein white matter immunoreactivity and sparse perivascular mononuclear cell cuffing. Four subjects (20%) had SCV2 RNA in one or more brain regions including the olfactory bulb, amygdala, entorhinal area, temporal and frontal neocortex, dorsal medulla and leptomeninges. The subject with encephalitis was SCV2-positive in a histopathologically-affected area, the entorhinal cortex, while the subject with the large acute cerebral infarct was SCV2-negative in all brain regions. Like other human coronaviruses, SCV2 can inflict acute neuropathology in susceptible patients. Much remains to be understood, including what viral and host factors influence SCV2 brain invasion and whether it is cleared from the brain subsequent to the acute illness.
Genome‐wide association studies of 27 accelerometry‐derived physical activity measurements identified novel loci and genetic mechanisms

Physical inactivity (PA) is an important risk factor for a wide range of diseases. Previous genome‐wide association studies (GWAS), based on self‐reported data or a small number of phenotypes derived from accelerometry, have identified a limited number of genetic loci associated with habitual PA and provided evidence for involvement of central nervous system in mediating genetic effects. In this study, we derived 27 PA phenotypes from wrist accelerometry data obtained from 88,411 UK Biobank study participants. Single‐variant association analysis based on mixed‐effects models and transcriptome‐wide association studies (TWAS) together identified 5 novel loci that were not detected by previous studies of PA, sleep duration and self‐reported chronotype. For both novel and previously known loci, we discovered associations with novel phenotypes including active‐to‐sedentary transition probability, light‐intensity PA, activity during different times of the day and proxy phenotypes to sleep and circadian patterns. Follow‐up studies including TWAS, colocalization, tissue‐specific heritability enrichment, gene‐set enrichment and genetic correlation analyses indicated the role of the blood and immune system in modulating the genetic effects and a secondary role of the digestive and endocrine systems. Our findings provided important insights into the genetic architecture of PA and its underlying mechanisms.
An improved algorithm for the three-fluid-phase VLLE flash calculation

The VLLE flash is important in water and hydrocarbons mixtures, hydrocarbon and CO2 rich mixtures, and hydrocarbon methane rich mixtures that are encountered in reservoir performance and recovery studies. A robust VLLE flash algorithm is proposed. The equilibrium and mass balance equations are solved as a constrained minimization problem. An inverse barrier function is used to handle the inequality constrains to solve for the phase fractions. It warrants always arriving to the solution. The challenging cases analyzed showed that the initialization procedure proposed, together with successive substitution iteration in the outer loop, is a good method for a stable VLLE flash algorithm, even near critical points. Whenever the result is in the region outside the three-phase physical domain, the solution suggests that the system has fewer phases. In one of the cases analyzed, a region with three liquid phases was encountered and the algorithm found two different solutions with positive phase fractions. © 2015 American Institute of Chemical Engineers AIChE J, 61: 3081–3093, 2015
The Use of Military Profiles in Romance Fraud Schemes

ABSTRACT Romance fraud occurs when offenders use the guise of a genuine relationship to defraud a victim, typically via online communications. Research has focused on the general structure of romance frauds, but few scholars have considered the potential link between victimization and specific narratives employed by offenders. This article explores the reported romance fraud victimizations of a group of Australians and explores similarities and differences in how a military narrative is involved compared to other genres. Limited differences were observed between those frauds using military narratives, highlighting the need for broader consideration of fraud prevention and disruption measures.
A Separated-Flow Model for Predicting Flow Boiling Critical Heat Flux and Pressure Drop Characteristics in Microchannels

Two-phase microchannel cooling promises high heat flux removal for high-performance electronics and photonics. However, the heat transfer performance of flow boiling microchannels is limited by the critical heat flux (CHF) conditions. For variable heat inputs and variable fluid flows, it is essential to predict CHFs accurately for effective and efficient two-phase microchannel cooling. To characterize the CHF and pressure drop in flow boiling microchannels, a separated-flow model is proposed in this paper based on fundamental two-phase flow mass, energy, momentum conservation and wall energy conservation laws. With this theoretical framework, the relationship among liquid/vapor interfacial instability, two-phase flow characteristics and CHF is further studied. This mechanistic model also provides insight into the design and operational guidelines for advanced electronics and photonics cooling technologies.Copyright © 2012 by ASME
Becoming “Hijas de la Lucha”: Political subjectification, affective intensities, and historical narratives in a Chilean all-girls high school

ABSTRACT This article explores the processes by which Chilean female feminist public high school students used political and historical narratives and symbols during the feminist movement of 2018. It analyzes how these particular usages were crossed by affective intensities that worked to produce students’ political subjectivities as collective and agentic. This affective work was produced with other members of the school community and sometimes was met with anger, policing practices, and desires to exclude students from particular historical narratives. The data for this article were produced within a yearlong critical ethnographic study on the processes of production of high school feminist students’ gender and political subjectivities in Chile, which included participant observation, testimonios interviews, art-based collective testimonios workshops, and analysis through affect theory. The findings show the types of historical and political narratives and symbols used by students, what affective intensities that crossed these symbols did to the students’ political subjectivities, and the conflicts these intensities produced within the school community. The article concludes by examining how these findings might open new questions within the field of social studies education research.
Pediatric demyelinating disease and anti‐MOG antibody

Significant progress has been made in the field of pediatric neuroimmunology over the past few years. With the detection of conformationally correct myelin oligodendrocyte glycoprotein (MOG) antibodies using cell‐based assays, children with different monophasic and relapsing phenotypes have been increasingly described. However, there is still controversy regarding the severity of the disease course and outcome of this emerging and rapidly expanding spectrum of MOG‐associated demyelination. Accumulating evidence seems to identify MOG‐IgG–associated disorder as a disease entity different from multiple sclerosis and aquaporin 4 (AQP4)‐IgG–positive neuromyelitis optica spectrum disorder. This review will summarize recent findings regarding the spectrum of MOG‐associated disorders in pediatric patients related to diagnosis, clinical presentation, and neuroimaging patterns, including data on current available and emerging treatment options for children.
COVID-19 and the Chinese economy: impacts, policy responses and implications

ABSTRACT COVID-19, the disease caused by novel coronavirus SARS-CoV-2, has greatly affected financial markets, economies and societies worldwide. This study focuses on China and examines a series of issues including: the impact of COVID-19 on the Chinese economy, China’s policy responses to this shock such as fiscal, monetary and institutional measures, and implications such as the nature, gains and costs of China’s policy responses. This study also explores problems that need to be answered in the future. In view of the importance of China in the world regarding the size of its economy, its contribution to world growth, and its increasing influence, this study makes timely and important contributions to policy makers and investors around the world.
Effects of Subsurface Drip Irrigation on Water Consumption and Yields of Alfalfa under Different Water and Fertilizer Conditions

A field experiment was conducted for the purpose of examining the effects of different combinations of water and fertilizer applications on the water consumption and yields of alfalfa under subsurface drip irrigation (SDI). The results showed that the jointing and branching stages were the key stages for alfalfa water requirement. The water consumption had varied greatly (from 130 to 170 mm) during the growth period of each alfalfa crop. The water consumption during the whole growth period was approximately 500 mm, and the maximum water consumption intensity was 3.64 mm·d-1. The overall changes in water consumption and yields during the growth period of the alfalfa displayed trends of first increasing and then decreasing. The sensitivities of the yields to water changes were much higher than that of fertilizer. The water use efficiency (WUE) of the alfalfa was determined to range from 1.68 to 3.20 kg·m-3, and the rate of growth had ranged from 4.85% to 51.77%. The WUE and rate of growth of the alfalfa indicated the following trend: second crop > third crop > first crop. The results of frequency analysis based on the water-nitrogen-yield regression equation are the following: irrigation amounts of 142~165 mm and nitrogen application of 61~80 kg·hm-2 have a 95% probability of obtaining a hay yield of alfalfa of more than 11903 kg·hm-2. These results suggest that SDI is a promising irrigation method, which can increase the WUE and hay yield of alfalfa under the condition of SDI within an appropriate amount of water and nitrogen fertilizer, and too low or too high water and nitrogen fertilizer will adversely affect the WUE and hay yield of alfalfa.
The Cholinergic System Contributes to the Immunopathological Progression of Experimental Pulmonary Tuberculosis

The cholinergic system is present in both bacteria and mammals and regulates inflammation during bacterial respiratory infections through neuronal and non-neuronal production of acetylcholine (ACh) and its receptors. However, the presence of this system during the immunopathogenesis of pulmonary tuberculosis (TB) in vivo and in its causative agent Mycobacterium tuberculosis (Mtb) has not been studied. Therefore, we used an experimental model of progressive pulmonary TB in BALB/c mice to quantify pulmonary ACh using high-performance liquid chromatography during the course of the disease. In addition, we performed immunohistochemistry in lung tissue to determine the cellular expression of cholinergic system components, and then administered nicotinic receptor (nAChR) antagonists to validate their effect on lung bacterial burden, inflammation, and pro-inflammatory cytokines. Finally, we subjected Mtb cultures to colorimetric analysis to reveal the production of ACh and the effect of ACh and nAChR antagonists on Mtb growth. Our results show high concentrations of ACh and expression of its synthesizing enzyme choline acetyltransferase (ChAT) during early infection in lung epithelial cells and macrophages. During late progressive TB, lung ACh upregulation was even higher and coincided with ChAT and α7 nAChR subunit expression in immune cells. Moreover, the administration of nAChR antagonists increased pro-inflammatory cytokines, reduced bacillary loads and synergized with antibiotic therapy in multidrug resistant TB. Finally, in vitro studies revealed that the bacteria is capable of producing nanomolar concentrations of ACh in liquid culture. In addition, the administration of ACh and nicotinic antagonists to Mtb cultures induced or inhibited bacterial proliferation, respectively. These results suggest that Mtb possesses a cholinergic system and upregulates the lung non-neuronal cholinergic system, particularly during late progressive TB. The upregulation of the cholinergic system during infection could aid both bacterial growth and immunomodulation within the lung to favor disease progression. Furthermore, the therapeutic efficacy of modulating this system suggests that it could be a target for treating the disease.
Interleukin-6 Is a Biomarker for the Development of Fatal Severe Acute Respiratory Syndrome Coronavirus 2 Pneumonia

Hyper-inflammatory responses induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are a major cause of disease severity and death. Predictive prognosis biomarkers to guide therapeutics are critically lacking. Several studies have indicated a “cytokine storm” with the release of interleukin-1 (IL-1), IL-6, and IL-8, along with tumor necrosis factor alpha (TNFα) and other inflammatory mediators. Here, we proposed to assess the relationship between IL-6 and outcomes of patients with coronavirus disease 2019 (COVID-19). Our cohort consisted of 46 adult patients with PCR-proven SARS-CoV-2 infection admitted in a COVID-19 ward of the Hospital de Braga (HB) from April 7 to May 7, 2020, whose IL-6 levels were followed over time. We found that IL-6 levels were significantly different between the disease stages. Also, we found a significant negative correlation between IL-6 levels during stages IIb and III, peripheral oxygen saturation (SpO2), and partial pressure of oxygen in arterial blood (PaO2), showing that IL-6 correlates with respiratory failure. Compared to the inflammatory markers available in the clinic routine, we found a positive correlation between IL-6 and C-reactive protein (CRP). However, when we assessed the predictive value of these two markers, IL-6 behaves as a better predictor of disease progression. In a binary logistic regression, IL-6 level was the most significant predictor of the non-survivors group, when compared to age and CRP. Herein, we present IL-6 as a relevant tool for prognostic evaluation, mainly as a predictor of outcome.
Volatile organic compound levels at one site in Rome urban air

Abstract Volatile organic compound (VOC), nitrogen dioxide (NO2) and ozone (O3) concentrations were measured at one site in Rome urban air during 2011. The seasonal mean concentrations of VOCs varied from 78 μg m−3 in winter to 37 μg m−3 in summer. Total aromatic concentration was reduced by 59% during summertime, alkanes of 39% and alkenes of 71%. VOC diurnal pattern exhibited a primary peak during the morning and a secondary peak in the evening hours coinciding with rush–hour traffic. The high correlation between benzene and toluene evidenced their common origin probably due to vehicular traffic. In summer isoprene diurnal profile showed both biogenic and anthropogenic origin. NO2 and O3 daily trends during summertime evidenced both photostationary state typical conditions and photochemical smog episodes. VOC and O3 trends also evidenced a reduction in VOC levels during O3 formation. Based on the Maximum Incremental Reactivity scale, the highest contributors to ozone production in Rome were propene, ethene and toluene. Comparing data found in Rome at the same site in 1992, 2007 and 2011, a decreasing trend in VOC levels was observed, suggesting the effectiveness of European Directives on air quality. In addition, our results were confirmed by similar data found in other urban areas around the world.
Evaluation of SNA001, a Novel Recombinant Human Thyroid Stimulating Hormone Injection, in Patients With Differentiated Thyroid Carcinoma

SNA001 is a novel recombinant human thyroid stimulating hormone (rhTSH). rhTSH has long been approved in several countries to facilitate monitoring and ablation of thyroid carcinoma without hypothyroidism caused by thyroid hormone withdrawal (THW). To assess the safety, tolerance, pharmacokinetic and pharmacodynamic properties of SNA001, the two-period (SNA001 period and THW period), dose-ascending study in well-differentiated thyroid cancer (DTC) patients was designed. Three doses (0.45 mg, 0.9 mg, and 1.35 mg) of SNA001 were intramuscularly injected, twice in the SNA001 period to stimulate iodine-131 uptake and thyroglobulin (Tg) release. 24 h after the last dose of SNA001, iodine-131 (111–185 MBq) was administrated, followed by whole-body scan (WBS) 48 h later. THW period began just after SNA001 washout and lasted for about 3–6 weeks. When TSH level was above 30 mU/L, iodine-131 (111–185 MBq) was administrated, followed by a WBS and Tg detection 48 h later. Twenty-four DTC patients after thyroidectomy were enrolled; mean peak concentrations of SNA001 in 0.45, 0.9, and 1.35 mg groups were 18.5, 26.7, and 37.0 ng/ml (about 244.7, 354.2, and 489.6 mU/L) respectively, within 28–32 h after first dose of SNA001. SNA001 was metabolized in a dose-dependent manner. The results of WBS and Tg release in the SNA001 period were compared with those in the THW period. Compared to Tg level in baseline, the Tg levels in SNA001 and THW periods were increased, with 78% of subjects showing higher Tg levels in the THW period. 100% of the patients had concordant qualitative results of the scans within two periods in three groups. Symptoms of hypothyroidism were relieved in the SNA001 period compared with THW period, though there was no significant difference in most of the scale scores. There were no serious adverse events related to SNA001; the most common adverse events were gastrointestinal symptoms of mild and transient nature. Thus, SNA001 promises to be a safe and effective method to stimulate iodine-131 uptake and Tg secretion during monitoring and ablation for DTC without the disadvantages of incidental hypothyroidism.
Chronic and Acute Manipulation of Cortical Glutamate Transmission Induces Structural and Synaptic Changes in Co-cultured Striatal Neurons

In contrast to the prenatal topographic development of sensory cortices, striatal circuit organization is slow and requires the functional maturation of cortical and thalamic excitatory inputs throughout the first postnatal month. While mechanisms regulating synapse development and plasticity are quite well described at excitatory synapses of glutamatergic neurons in the neocortex, comparatively little is known of how this translates to glutamate synapses onto GABAergic neurons in the striatum. Here we investigate excitatory striatal synapse plasticity in an in vitro system, where glutamate can be studied in isolation from dopamine and other neuromodulators. We examined pre-and post-synaptic structural and functional plasticity in GABAergic striatal spiny projection neurons (SPNs), co-cultured with glutamatergic cortical neurons. After synapse formation, medium-term (24 h) TTX silencing increased the density of filopodia, and modestly decreased dendritic spine density, when assayed at 21 days in vitro (DIV). Spine reductions appeared to require residual spontaneous activation of ionotropic glutamate receptors. Conversely, chronic (14 days) TTX silencing markedly reduced spine density without any observed increase in filopodia density. Time-dependent, biphasic changes to the presynaptic marker Synapsin-1 were also observed, independent of residual spontaneous activity. Acute silencing (3 h) did not affect presynaptic markers or postsynaptic structures. To induce rapid, activity-dependent plasticity in striatal neurons, a chemical NMDA receptor-dependent “long-term potentiation (LTP)” paradigm was employed. Within 30 min, this increased spine and GluA1 cluster densities, and the percentage of spines containing GluA1 clusters, without altering the presynaptic signal. The results demonstrate that the growth and pruning of dendritic protrusions is an active process, requiring glutamate receptor activity in striatal projection neurons. Furthermore, NMDA receptor activation is sufficient to drive glutamatergic structural plasticity in SPNs, in the absence of dopamine or other neuromodulators.