Miracle-dz/UniFAR-FTUs huggingface dataset:
Crosstalk between Mast Cells and Lung Fibroblasts Is Modified by Alveolar Extracellular Matrix and Influences Epithelial Migration

Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial cells in idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate the effect of mast cells on fibroblast activity and migration of epithelial cells. Lung fibroblasts from IPF patients and healthy individuals were co-cultured with LAD2 mast cells or stimulated with the proteases tryptase and chymase. Human lung fibroblasts and mast cells were cultured on cell culture plastic plates or decellularized human lung tissue (scaffolds) to create a more physiological milieu by providing an alveolar extracellular matrix. Released mediators were analyzed and evaluated for effects on epithelial cell migration. Tryptase increased vascular endothelial growth factor (VEGF) release from fibroblasts, whereas co-culture with mast cells increased IL-6 and hepatocyte growth factor (HGF). Culture in scaffolds increased the release of VEGF compared to culture on plastic. Migration of epithelial cells was reduced by IL-6, while HGF and conditioned media from scaffold cultures promoted migration. In conclusion, mast cells and tryptase increased fibroblast release of mediators that influenced epithelial migration. These data indicate a role of mast cells and tryptase in the interplay between fibroblasts, epithelial cells and the alveolar extracellular matrix in health and lung disease.
Genetics and Genomics of SOST: Functional Analysis of Variants and Genomic Regulation in Osteoblasts

SOST encodes the sclerostin protein, which acts as a key extracellular inhibitor of the canonical Wnt pathway in bone, playing a crucial role in skeletal development and bone homeostasis. The objective of this work was to assess the functionality of two variants previously identified (the rare variant rs570754792 and the missense variant p.Val10Ile) and to investigate the physical interactors of the SOST proximal promoter region in bone cells. Through a promoter luciferase reporter assay we show that the minor allele of rs570754792, a variant located in the extended TATA box motif, displays a significant decrease in promoter activity. Likewise, through western blot studies of extracellular and intracellular sclerostin, we observe a reduced expression of the p.Val10Ile mutant protein. Finally, using a circular chromosome conformation capture assay (4C-seq) in 3 bone cell types (MSC, hFOB, Saos-2), we have detected physical interactions between the SOST proximal promoter and the ECR5 enhancer, several additional enhancers located between EVT4 and MEOX1 and a distant region containing exon 18 of DHX8. In conclusion, SOST presents functional regulatory and missense variants that affect its expression and displays physical contacts with far reaching genomic sequences, which may play a role in its regulation within bone cells.
How Does High-Performance Work System Prompt Job Crafting through Autonomous Motivation: The Moderating Role of Initiative Climate

By invoking self-determination theory, we proposed an integrated, multilevel model to investigate the impact of a high-performance work system (HPWS) on employees’ job crafting through autonomous motivation, along with the moderation effect of initiative climate. Adopting a three-wave, time-lagged research design, we collected data from 615 employees of 54 Chinese companies. The results of multilevel path analysis revealed that (1) HPWS is positively related to employees’ job crafting; (2) HPWS has a positive impact on employees’ autonomous motivation; (3) employees’ autonomous motivation positively affects their job crafting; (4) employees’ autonomous motivation mediates the positive relationship between HPWS and employees’ job crafting; (5) initiative climate moderates the relationship between employees’ autonomous motivation and job crafting; and (6) the indirect relationship between HPWS and job crafting through autonomous motivation is also moderated by initiative climate. The findings of this study provided several implications for job crafting research and for human resource management in organizations.
Associations between Potentially Modifiable and Non-Modifiable Risk Factors and Gait Speed in Middle and Older-Aged Adults: Results from the Canadian Longitudinal Study on Aging.

BACKGROUND Gait speed is a strong predictor of morbidity and mortality in older adults. Understanding the factors associated with gait speed and the associated adverse outcomes will inform mitigation strategies. We assessed the potentially modifiable and non-modifiable factors associated with gait speed in a large national cohort of middle and older-aged Canadian adults. METHODS We examined cross-sectional baseline data from the Canadian Longitudinal Study on Aging (CLSA) Comprehensive cohort. The study sample included 20,201 community-dwelling adults aged 45 to 85 years. The associations between sociodemographic and anthropometric factors, chronic conditions, and cognitive, clinical, and lifestyle factors and 4-meter usual gait speed (m/s) were estimated using hierarchical multivariable linear regression. RESULTS The coefficient of determination, R 2, of the final regression model was 19.7%, with 12.9% of gait speed variability explained by sociodemographic and anthropometric factors, and non-modifiable chronic conditions and 6.8% explained by potentially modifiable chronic conditions, cognitive, clinical, and lifestyle factors. Potentially modifiable factors significantly associated with gait speed include cardiovascular conditions (unstandardized regression coefficient, B= -.018; p<.001), stroke (B= -.025; p=.003), hypertension (B= -.007; p=.026), serum Vitamin D (B= .004; p<.001), C-Reactive protein (B= -.005; p=.005), depressive symptoms (B= -.003; p<.001), physical activity (B= .0001; p<.001), grip strength (B= .003; p<.001), current smoking (B= -.026; p<.001), severe obesity (B= -.086; p<.001), and chronic pain (B= -.008; p=.018). CONCLUSIONS The correlates of gait speed in adulthood are multifactorial, with many being potentially modifiable through interventions and education. Our results provide a life-course-perspective framework for future longitudinal assessments risk factors affecting gait speed.
Validation of Clinical Characteristics and Effectiveness of Pulmonary Rehabilitation in a COPD Population with Discrepancy between Exercise Tolerance and FEV1

This study’s objective was to examine the characteristics of patients with chronic obstructive pulmonary disease (COPD) presenting with various exercise tolerance levels. A total of 235 patients with stable COPD were classified into 4 groups: (1) LoFlo + HiEx—patients with a six-minute walking distance (6MWD) ≥350 m and percentage of predicted forced expiratory volume in 1 s (%FEV1.0) <50%; (2) HiFlo + HiEx—patients with a 6MWD ≥350 m and a %FEV1.0 ≥50%; (3) LoFlo + LoEx—patients with a 6MWD < 350 m and %FEV1.0 < 50%; and (4) HiFlo + LoEx—patients with a 6MWD <350 m and %FEV1.0 ≥ 50%. Aspects of physical ability in the HiFlo + LoEx group were significantly lower than those in the HiFlo + HiEx group. The HiFlo + LoEx group was characterized by a history of hospitalization for respiratory illness within the past year, treatment with at-home oxygen therapy, and lacking daily exercise habits. Following three months of pulmonary rehabilitation, the LoFlo + HiEx group significantly improved in the modified Medical Research Council dyspnea score, maximum gait speed, and 6MWD, while the HiFlo + LoEx group significantly improved in the percentage of maximal expiratory pressure, maximum gait speed, 6MWD, incremental shuttle walking distance, and St. George’s Respiratory Questionnaire score. The HiFlo + LoEx group had the greatest effect of three-month pulmonary rehabilitation compared to other groups.
Performance Characteristics of the Vidas SARS-CoV-2 IgM and IgG Serological Assays

The COVID-19 pandemic, caused by the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to spread worldwide. Serological testing for SARS-CoV-2-specific antibodies plays an important role in understanding and controlling the pandemic, notably through epidemiological surveillance. ABSTRACT The COVID-19 pandemic, caused by the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to spread worldwide. Serological testing for SARS-CoV-2-specific antibodies plays an important role in understanding and controlling the pandemic, notably through epidemiological surveillance. Well-validated and highly specific SARS-CoV-2 serological assays are urgently needed. We describe here the analytical and clinical performance of Vidas SARS-CoV-2 IgM and Vidas SARS-CoV-2 IgG, two CE-marked, emergency use authorization (EUA)-authorized, automated, qualitative assays for the detection of SARS-CoV-2-specific IgM and IgG, respectively. Both assays showed high within-run and within-laboratory precision (coefficients of variation < 11.0%) and very low cross-reactivity toward sera of patients with a past common coronavirus or respiratory virus infection. Clinical specificity determined on up to 989 prepandemic healthy donors was ≥99% with a narrow 95% confidence interval for both IgM and IgG assays. Clinical sensitivity was determined on up to 232 samples from 130 reverse transcriptase PCR (RT-PCR)-confirmed SARS-CoV-2 patients. The positive percent agreement (PPA) with SARS-CoV-2 PCR reached 100% at ≥16 days (Vidas SARS-CoV-2 IgM) and ≥32 days (Vidas SARS-CoV-2 IgG) of symptom onset. Combined IgM/IgG test results improved the PPA compared to each test alone. SARS-CoV-2 IgG seroconversion followed closely that of SARS-CoV-2 IgM and remained stable over time, while SARS-CoV-2 IgM levels rapidly declined. Interestingly, SARS-CoV-2-specific IgM and IgG responses were significantly higher in COVID-19 hospitalized versus nonhospitalized patients. Altogether, the Vidas SARS-CoV-2 IgM and IgG assays are highly specific and sensitive serological tests suitable for the reliable detection of past acute SARS-CoV-2 infections.
A Systematic Review of Adolescent Masculinities and Associations with Internalizing Behavior Problems and Social Support

Abstract Interest in the connection between masculinities and mental health continues to grow. However, no previous systematic review has explored this association for adolescents. We present the systematic review of 29 articles that explore the connection between adherence to stereotypical male gender role norms (e.g., emotional restriction), attributes (e.g., “ambitious”), and identity (most commonly, gender “typicality”) and internalizing behavior problems and social support. A total of 24,795 adolescent boys (6th‐12th grade) were included in the reviewed studies from 1997–2017. In the quantitative articles (n = 20), associations varied by aspect of masculinity assessed. Specifically, we found that greater endorsement of “masculine” traits (e.g., ambitious, assertive) was generally associated with fewer internalizing behavior problems and greater social support. However, lower gender “typicality” and higher adherence to stereotypical gender role norms were generally associated with more internalizing behavior problems and lower social support. In the qualitative articles (n = 9), the most predominant theme was emotional restriction (i.e., a gender role norm) and consequences for mental health. While research in this area is newer for community psychologists, the connection between masculinities and mental health is directly relevant to the field. Given the focus on individual‐level conceptions of masculinity and mental health found in our review, we describe key future directions for masculinities research in community psychology.
A sound coding strategy based on a temporal masking model for cochlear implants

Auditory masking occurs when one sound is perceptually altered by the presence of another sound. Auditory masking in the frequency domain is known as simultaneous masking and in the time domain is known as temporal masking or non-simultaneous masking. This works presents a sound coding strategy that incorporates a temporal masking model to select the most relevant channels for stimulation in a cochlear implant (CI). A previous version of the strategy, termed psychoacoustic advanced combination encoder (PACE), only used a simultaneous masking model for the same purpose, for this reason the new strategy has been termed temporal-PACE (TPACE). We hypothesized that a sound coding strategy that focuses on stimulating the auditory nerve with pulses that are as masked as possible can improve speech intelligibility for CI users. The temporal masking model used within TPACE attenuates the simultaneous masking thresholds estimated by PACE over time. The attenuation is designed to fall exponentially with a strength determined by a single parameter, the temporal masking half-life T½. This parameter gives the time interval at which the simultaneous masking threshold is halved. The study group consisted of 24 postlingually deaf subjects with a minimum of six months experience after CI activation. A crossover design was used to compare four variants of the new temporal masking strategy TPACE (T½ ranging between 0.4 and 1.1 ms) with respect to the clinical MP3000 strategy, a commercial implementation of the PACE strategy, in two prospective, within-subject, repeated-measure experiments. The outcome measure was speech intelligibility in noise at 15 to 5 dB SNR. In two consecutive experiments, the TPACE with T½ of 0.5 ms obtained a speech performance increase of 11% and 10% with respect to the MP3000 (T½ = 0 ms), respectively. The improved speech test scores correlated with the clinical performance of the subjects: CI users with above-average outcome in their routine speech tests showed higher benefit with TPACE. It seems that the consideration of short-acting temporal masking can improve speech intelligibility in CI users. The half-live with the highest average speech perception benefit (0.5 ms) corresponds to time scales that are typical for neuronal refractory behavior.
The influence of marital status and partner concordance on participation in colorectal cancer screening.

BACKGROUND Colorectal cancer screening program using a fecal immunochemical test aims to reduce morbidity and mortality through early detection. Although screening participation is free-of-charge, almost 40% of the invited individuals choose not to participate. To bring new insight into how non-participation can be identified and targeted, we examined the association between marital status and screening participation; with a focus on partner concordance in participation and sex differences. METHODS This nationwide cross-sectional study included all Danish citizens aged 50-74 years, who were invited to colorectal cancer screening between 2014 and 2017. Logistic regression analysis was used to estimate odds ratio (OR) of participation while adjusting for sociodemographic variables. RESULTS A total of 1 909 662 individuals were included in the analysis of which 62.7% participated in the screening program. Participation was highest among women. Stratified by marital status, screening participation was markedly lower in widowed (61.5%), divorced (54.8%) and single (47.3%), while participation reached 68.4% in married individuals. This corresponded to ORs of 0.59 (95% CI 0.58-0.59) for widowed, 0.56 (95% CI 0.55-0.56) for divorced and 0.47 (95% CI 0.47-0.48) for single, compared to married individuals. Individuals married to a participating partner were five times more likely to participate than married individuals with a non-participating partner, regardless of gender. CONCLUSIONS Marital status was strongly associated with participation in colorectal cancer screening, and participation was even higher in married individuals with a participating partner. Future efforts to increase participation in colorectal cancer screening could potentially benefit from considering the role of partner concordance.
Exotic foods reveal contact between South Asia and the Near East during the second millennium BCE

Significance Here we report the identification of staple and exotic food remains in Bronze and Early Iron Age dental calculus from the Southern Levant. The analysis of dietary plant microremains and proteins sheds new light on consumed exotic foods from South and East Asia during the second millennium BCE. We provide the earliest direct evidence in the Mediterranean to date for the consumption of sesame, soybean, probable banana, and turmeric. The recovery and identification of diverse foodstuffs using molecular and microscopic techniques enables a new understanding of the complexity of early trade routes and nascent globalization in the ancient Near East and raises questions about the long-term maintenance and continuity of this trade system into later periods. Although the key role of long-distance trade in the transformation of cuisines worldwide has been well-documented since at least the Roman era, the prehistory of the Eurasian food trade is less visible. In order to shed light on the transformation of Eastern Mediterranean cuisines during the Bronze Age and Early Iron Age, we analyzed microremains and proteins preserved in the dental calculus of individuals who lived during the second millennium BCE in the Southern Levant. Our results provide clear evidence for the consumption of expected staple foods, such as cereals (Triticeae), sesame (Sesamum), and dates (Phoenix). We additionally report evidence for the consumption of soybean (Glycine), probable banana (Musa), and turmeric (Curcuma), which pushes back the earliest evidence of these foods in the Mediterranean by centuries (turmeric) or even millennia (soybean). We find that, from the early second millennium onwards, at least some people in the Eastern Mediterranean had access to food from distant locations, including South Asia, and such goods were likely consumed as oils, dried fruits, and spices. These insights force us to rethink the complexity and intensity of Indo-Mediterranean trade during the Bronze Age as well as the degree of globalization in early Eastern Mediterranean cuisine.
Quantification of Metastatic Prostate Cancer Whole-Body Tumor Burden with 18F-FDG PET Parameters and Associations with Overall Survival After First-Line Abiraterone or Enzalutamide: A Single-Center Retrospective Cohort Study

Visual Abstract New biomarkers for metastatic prostate cancer are needed. The aim of this study was to evaluate the prognostic value of 18F-FDG PET whole-body tumor burden parameters in patients with metastatic prostate cancer who received first-line abiraterone or enzalutamide therapy. Methods: This was a retrospective study of patients with metastatic castration-sensitive prostate cancer (mCSPC, n = 25) and metastatic castration-resistant prostate cancer (mCRPC, n = 71) who underwent 18F-FDG PET/CT within 90 d before first-line treatment with abiraterone or enzalutamide at a tertiary-care academic cancer center. Whole-body tumor burden on PET/CT was quantified as metabolic tumor volume (MTV) and total lesion glycolysis (TLG) and correlated with overall survival (OS) probabilities using Kaplan–Meier curves and Cox models. Results: The median follow-up in survivors was 56.3 mo (interquartile range, 37.7–66.8 mo); the median OSs for patients with mCRPC and mCSPC were 27.8 and 76.1 mo, respectively (P < 0.001). On univariate analysis, the OS probability of mCRPC patients was significantly associated with plasma levels of alkaline phosphatase (hazard ratio [HR], 1.90; P < 0.001), plasma levels of lactate dehydrogenase (HR, 1.01; P < 0.001), hemoglobin levels (HR, 0.80; P = 0.013), whole-body SUVmax (HR, 1.14; P < 0.001), the number of 18F-FDG–avid metastases (HR, 1.08; P < 0.001), whole-body metabolic tumor volume (HR, 1.86; P < 0.001), and TLG (HR, 1.84; P < 0.001). On multivariable analysis with stepwise variable selection, hemoglobin levels (HR, 0.81; P = 0.013) and whole-body TLG (HR, 1.88; P < 0.001) were independently associated with OS. In mCSPC patients, no significant association was observed between these variables and OS. Conclusion: In patients with mCRPC receiving first-line treatment with abiraterone or enzalutamide, 18F-FDG PET WB TLG is independently associated with OS and might be used as a quantitative prognostic imaging biomarker.
Differences in Tibia Shape in Organically Reared Chicken Lines Measured by Means of Geometric Morphometrics

Simple Summary Organic poultry production should use only genetic lines and animals resistant to disease and well adapted to live outdoor, according to principles, rules, and requirements of organic farming systems. When broiler’s walking performance is reduced animals are not suitable for outdoor rearing. There is a straightforward relationship between bone health and growth rate in broilers. Body and breast weight play an important role in leg disorders. During the last decades, genetic selection has led to high producing broilers over the time. Unfortunately, fast growth may negatively influence correct leg development, reducing walking performance, and raising welfare issues. Leg abnormalities could represent a criterion for the choice of genetic lines suitable for organic production. A method for their early detection was developed in this study by means of Geometric Morphometrics (GM) that represents a tool for bone shape analysis and its correlation with walking capability. A valuable information emerged from the present study in relation to broiler intrinsic adaptability to organic production. Abstract In the present study, the conformation of the tibia of seven genetic lines of broilers was analyzed by Geometric Morphometrics and correlated to carcass weight and walking ability. The used chicken genetic lines were classified as fast, medium, or slow growing and ranked for their walking ability. Six chicken types were reared in an organic farm and slaughtered at 81 days of age while one slow-growing and highly walking line (Naked Neck) was reared in a commercial farm and used as external reference for moving activity and growth speed. A mixed landmarks and semi-landmarks model was applied to the study of tibia shape. Results of this study showed that: (i) body weight gain was positively correlated to the curvature of the antero-posterior axis of the tibia; (ii) the shape of the tibia and the active walking behavior were significantly correlated; (iii) walking and not-walking genetic lines could be discriminated in relation to the overall shape of the tibia; (iv) a prevalence of static behavior was correlated to a more pronounced curvature of the antero-posterior axis of the tibia. Results of this study revealed that the walking genetic types have a more functional and natural tibia conformation. This easy morphologic method for evaluating tibia shape could help to characterize the adaptability of genotypes to organic and outdoor rearing.
Descoloração e degradação do azo corante Vermelho GRLX-220 por ozonização

The discoloration and degradation of the textile dye RED GRLX-220 using the electrochemically generated ozone was investigated. Total discoloration was rapidly achieved in both acid and basic conditions. A pseudo-first order kinetics was observed for discoloration, influenced by pH and ozonation time. A considerable degree of mineralization (60%) was obtained after 30 min of ozonation in alkaline medium. The feasibility of organic matter oxidation during the ozonation process increased in both acidic and alkaline media. The toxicity decreased after the ozonation process, suggesting that the byproducts are less toxic than the parental compound.