Miracle-dz/UniFAR-FTUs huggingface dataset:
Monitoring of Donor‐Derived Cell‐Free DNA by Short Tandem Repeats: Concentration of Total Cell‐Free DNA and Fragment Size for Acute Rejection Risk Assessment in Liver Transplantation

Monitoring of graft function is essential during the first months after liver transplantation (LT), but current liver function tests (LFTs) lack the specificity and sensitivity to ensure an efficient diagnosis of acute rejection (AR). Recently, donor‐derived cell‐free DNA (ddcfDNA) has emerged as a noninvasive biomarker to assess graft integrity. This study evaluated the feasibility of measuring the ddcfDNA through short tandem repeat (STR) analysis by quantitative fluorescent‐polymerase chain reaction (QF‐PCR) and to assess the role of the concentration and fragment size of total cfDNA as AR biomarkers. The total concentration and fragment size of cfDNA and the ddcfDNA percentage were monitored in plasma of 20 patients without rejection and 7 patients with T‐cell–mediated AR during the first 3 months after LT. The median ddcfDNA percentage was 3‐fold higher before AR diagnosis (34.8%; P < 0.001) and moderately higher at AR confirmatory diagnosis (23.8%; P = 0.049) compared with that of nonrejector patients (10.6%), showing a better performance (area under the curve = 84.6%) than conventional LFTs to predict the risk of rejection within the first 2 weeks following LT. The fraction of 100‐250‐bp cfDNA fragments was higher at AR diagnosis compared with that of nonrejector patients (68.0% versus 57.9%, P = 0.02). STR amplification by QF‐PCR may be an alternative strategy for rapid ddcfDNA quantification, which is easily implementable in clinical laboratories. The results of this pilot study indicate that ddcfDNA increases very early, even 1‐2 weeks before the diagnosis of AR, and so it could be useful as a prognostic biomarker in improving patient risk stratification.
Key Data for Ionizing-Radiation Dosimetry: Measurement Standards and Applications

Ionizing radiation dosimetry is used to describe those measurements or calculations that provide information on the energy deposited by the interaction of ionizing radiation with matter. Radiation dosimetry is of importance in several areas, including radiation therapy, radiation protection, and the industrial use of radiation. Several techniques have been developed for measuring energy deposition, with ionization chambers and calorimeters being the most important. These techniques form the basis of primary measurement standards for ionizing radiation, and these primary standards require values and uncertainty estimates for certain key parameters used to relate the result of measurement to the desired quantity. The calculation of the interaction of radiation with matter using Monte Carlo techniques is now well developed and can be used to study energy deposition for problems where measurements are difficult or impossible. Both measurements and calculations require knowledge of basic quantities related to the interaction of radiation with matter. These include data on photon cross sections, electron stopping powers, and the average energy to create an ion pair, to name a few. These data are often referred to as “key data. ” This Report examines key data for stopping powers for charged particles ranging from electrons to carbon ions. Values and uncertainties are assigned to the mean excitation energies for air, graphite, and liquid water, and tables of stopping powers covering the energy range from 1 keV to 1 GeV, or higher, are provided. Photon cross sections for air, water, and graphite are reviewed, examined, and compared with relevant measurements to estimate their uncertainties. Values are recommended for the average energy to create an ion pair in air, Wair. The available data for the chemical yield for Fricke dosimetry and for the heat defects for graphite and liquid water are summarized, as is the humidity correction factor for air-filled ionization chambers and the correction to the measured charge due to the initial ion pairs created by an incident photon. Data for the correction in photon and electron beams at low energies for the deviation of Wair from the recommended asymptotic value are also summarized. The impact of the recommended changes is discussed. Important changes are an increase in the uncertainty for air-kerma measurements with free-air chambers and a decrease of about 0.7 % in 60Co air-kerma measurements. The recommended stopping powers for graphite and liquid water differ by up to 1 % from those previously recommended. For radiation dosimetry based on ionization chambers calibrated against absorbed-dose-to-water calorimeters, changes in the measured absorbed dose to water will not exceed 0.5%.
Recent Advances and Future Directions in Brain MR Imaging Studies in Schizophrenia: Toward Elucidating Brain Pathology and Developing Clinical Tools

Schizophrenia is a common severe psychiatric disorder that affects approximately 1% of general population through the life course. Historically, in Kraepelin’s time, schizophrenia was a disease unit conceptualized as dementia praecox; however, since then, the disease concept has changed. Recent MRI studies had shown that the neuropathology of the brain in this disorder was characterized by mild progression before and after the onset of the disease, and that the brain alterations were relatively smaller than assumed. Although genetic factors contribute to the brain alterations in schizophrenia, which are thought to be trait differences, other changes include factors that are common in psychiatric diseases. Furthermore, it has been shown that the brain differences specific to schizophrenia were relatively small compared to other changes, such as those caused by brain development, aging, and gender. In addition, compared to the disease and participant factors, machine and imaging protocol differences could affect MRI signals, which should be addressed in multi-site studies. Recent advances in MRI modalities, such as multi-shell diffusion-weighted imaging, magnetic resonance spectroscopy, and multimodal brain imaging analysis, may be candidates to sharpen the characterization of schizophrenia-specific factors and provide new insights. The Brain/MINDS Beyond Human Brain MRI (BMB-HBM) project has been launched considering the differences and noises irrespective of the disease pathologies and includes the future perspectives of MRI studies for various psychiatric and neurological disorders. The sites use restricted MRI machines and harmonized multi-modal protocols, standardized image preprocessing, and traveling subject harmonization. Data sharing to the public will be planned in FY 2024. In the future, we believe that combining a high-quality human MRI dataset with genetic data, randomized controlled trials, and MRI for non-human primates and animal models will enable us to understand schizophrenia, elucidate its neural bases and therapeutic targets, and provide tools for clinical application at bedside.
Comparing the clinical presentation and outcomes of dogs receiving medical or surgical treatment for osseous-associated cervical spondylomyelopathy.

BACKGROUND This study aimed to compare the clinical presentation, short and long term outcomes of dogs treated surgically or medically for Osseous associated cervical spondylomyelopathy. METHODS Information collected retrospectively from medical records included signalment, neurological status and treatment type. Surgical treatment consisted of dorsal laminectomy. Medical treatment involved restricted exercise and medication. Improvement or deterioration in neurological status was determined at discharge, re-examination 4-8 weeks post-treatment and by telephone interview with the referring veterinary surgeon or owner at the time of the study, which ranged from 8-54 months following the discharge (median, 16 months). RESULTS Twenty-four dogs were treated surgically and 30 medically. Neurological grade at clinical presentation was significantly higher in surgically treated dogs (p = 0.004). Transient early postoperative neurological deterioration occurred in 73.1% of surgically treated dogs. For medical cases, long term improvement was seen in 15% of cases, remained static in 40% and deterioration in 45%. Surgical treatment resulted in long term improvement in 67% of cases, remained static in 29% and deterioration in 4% of cases. CONCLUSION This study suggests that surgery is a favourable treatment option, however, requires intensive post-operative care. Medical treatment was associated with a guarded prognosis but could be a viable treatment option for selected dogs.
Effects of Elevated Atmospheric CO2 on Photosynthesis, Growth and Biomass in Shorea platycarpa F. Heim (Meranti Paya)

Elevated atmospheric CO2 is widely reported to stimulate the plant growth and affect plant physiological processes. However, studies on the respond of tropical plant species to elevated CO2 are quite limited and remain largely unknown. The objective of this study was to investigate the effects of elevated atmospheric CO2 treatments on the photosynthetic characteristics, growth and biomass in Shorea platycarpa. Saplings of S. platycarpa were grown for seven months in the open roof gas chamber supplied with elevated CO2 (800±50 μmol mol-1) and in the shade house with ambient CO2 (400±50 μmol mol-1). Measurements of S. platycarpa growth and photosynthetic characteristics were made at frequent intervals. Biomass characteristics were determined using destructive methods after seven months of treatment and nondestructive method was used for leaf area index (LAI) determination. Photosynthetic rate (A) of S. platycarpa was not significantly affected by elevated CO2. Increased water use efficiency (WUE) of S. platycarpa grown in elevated CO2 was due to the reduced stomatal conductance (gs) and transpiration rate (E). The CO2 elevation had no significant effect on the S. platycarpa relative growth rates (RGR) and biomass but significantly reduced the leaf area. A weak correlation was found between photosynthetic rate (A) and relative growth rate (RGR). The results clearly showed that photosynthesis, growth rate and biomass of S. platycarpa were not significantly enhanced by elevated CO2. The findings indicated that elevated CO2 did not affect a relatively slow growing and a late successional peat swamp tree species.
Psychedelic Knowledge and Opinions in Psychiatrists at Two Professional Conferences: An Exploratory Survey

ABSTRACT Despite resurgent interest in psychedelic-assisted therapy, our insights into psychiatrists’ knowledge and opinions about medicinal psychedelic applications are surprisingly narrow. Therefore, we anonymously surveyed psychiatrists attending psychedelic didactic presentations at two national meetings about these issues using a 26-item questionnaire. Response rate was 40.20% (106/264). Respondents were 41.73 ± 13.31 years old (range: 24–80) and 64.42% were male. They largely believed psychedelics show treatment promise and strongly supported federal funding for medicinal psychedelic research. The most common concerns were the lack of trained psychedelic-assisted therapy providers, the logistics of psychedelic-assisted therapy delivery, the administration of psychedelics for patients with contraindications, and diversion. The most desired psychedelic-related educational topics were potential benefits of psychedelic-assisted therapy, how to conduct psychedelic-assisted therapy, psychedelic pharmacology, and psychedelic side effects. Factors associated with increased belief in psychedelics’ treatment potential included working primarily in research, scoring higher on a psychedelic knowledge test, and reporting less concern about psychedelics’ addictive potential. Working primarily in research and consult-liaison psychiatry fellowship training were positively associated with support for medicinal psychedelic legalization, while increased concerns about addictive potential and attending psychiatrist status were negatively associated. Support for legalization of non-medicinal psychedelic use was negatively associated with age and positively associated with support for legalization of medicinal psychedelic use.
Metrological properties of neuropsychological tests for measuring cognitive change in individuals with prodromal Alzheimer’s disease

Abstract Objectives In Alzheimer’s Disease (AD) research, choosing appropriate method for measuring change in cognitive function over time can be challenging. The aim for this study was to examine the sensitivity of four neuropsychological tests used to measure cognition during the transition from mild cognitive impairment (MCI) to AD, and the impacts of associated covariates. Methods We enrolled 223 patients with MCI who progressed to AD and had completed multiple follow-up assessments in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database. We constructed nonlinear mixed model for multivariate longitudinal data assuming that multiple neuropsychological tests would exhibit nonlinear transformation of a common factor in the latent cognitive process underlying the progression from MCI to AD. Results The Clinical Dementia Rating-Sum of the Boxes (CDR-SB) and Alzheimer’s Disease Assessment Scale (11 items; ADAS-11) were more sensitive to cognitive changes in individuals with higher cognitive function, the Functional Activities Questionnaire (FAQ) was more sensitive to cognitive changes in individuals with middle cognitive function, and the Mini-Mental State Examination (MMSE) was more sensitive to cognitive changes in individuals with lower cognitive function. Gender (p = 0.0139) and educational level (p = 0.0094) had varying effects on different tests, such that men performed better on the FAQ and CDR-SB, and individuals with higher educational level tended to perform better on the FAQ and MMSE. Conclusions When choosing appropriate neuropsychological tests in cognitive measurements, the cognitive functional level of the patient as well as the impacts of covariates should be considered.
Ventrolateral temporal lobectomy in normal dogs as a counterpart to human anterior temporal lobectomy: a preliminary study on the surgical procedure and complications

Anterior temporal lobectomy (ATL) is a surgical procedure for drug-resistant mesial temporal lobe epilepsy that is commonly performed in human medicine. The purpose of this study was to determine whether ATL-like surgery, i.e., removal of the amygdala and hippocampal head, is possible in dogs, and to investigate its safety and postoperative complications. Eight healthy beagles underwent ATL-like surgery and were observed for 3 months postoperatively. Samples from the surgically resected tissues and postmortem brain were evaluated pathologically. The surgical survival rate was 62.5%. The major postoperative complications were visual impairment, temporal muscle atrophy on the operative side, and a postoperative acute symptomatic seizure. Due to the anatomical differences between dogs and humans, the surgically resected area to approach the medial temporal structures in dogs was the ventrolateral part of the temporal lobe. Therefore, the ATL-like surgery described in this study was named “ventrolateral temporal lobectomy” (VTL). This study is the first report of temporal lobectomy including amygdalohippocampectomy in veterinary medicine and demonstrates its feasibility. Although it requires some degree of skill, VTL could be a treatment option for canine drug-resistant epilepsy and lesions in the mesial temporal lobe.
Discrete Element Method–Based Investigations of Granular Flow in a Pebble Bed Reactor

Abstract In a pebble bed reactor (PBR) core, nuclear fuel in the form of pebbles moves slowly under the influence of gravity. Due to the dynamic nature of the core, a thorough understanding about slow and dense granular flow of pebbles is required from both a reactor safety point of view and a performance evaluation point of view. In the current study, validation of discrete element method (DEM)–based simulation for the pebble flow in a PBR was carried out. Validation of DEM-based simulations necessitates validation of the employed numerical method of simulating packed structure. Hence, a parametric sensitivity study of packing interaction properties was initially conducted and also validation of the numerical method simulating packed structure at first. The parametric sensitivity analysis suggests that static friction characteristics play an important role from a packed/pebble bed structural characterization point of view. In addition, the simulated packed structure approach has shown a good agreement with the available benchmark data. Afterward, the effect of two different half-cone angles of 30 deg and 60 deg on pebble flow field in a PBR was studied by EDEMTM-based simulations. Results of streamlines, velocity radial profiles, and direct observation of discharge indicated a plug-type flow in the upper cylindrical region, whereas results indicated converging-type flow near the bottom conical region. EDEMTM results of granular flow were validated against experimental benchmark data and show a fair agreement in terms of Lagrangian trajectories and velocity profile. Therefore, this validated EDEMTM-based simulation can be used to obtain reliable results of pebble dynamics in a PBR and to enhance understanding of this phenomenon in a PBR. However, additional experimental investigations are recommended to be carried out for different sizes of test reactors, different bottom cone angles, and different sizes of pebbles to further assess DEM simulation results before using them for full-scale reactor simulations.
Neural dynamics between anterior insular cortex and right supramarginal gyrus dissociate genuine affect sharing from perceptual saliency of pretended pain

Empathy for pain engages both shared affective responses and self-other distinction. In this study, we addressed the highly debated question of whether neural responses previously linked to affect sharing could result from the perception of salient affective displays. Moreover, we investigated how the brain network involved in affect sharing and self-other distinction underpinned our response to a pain that is either perceived as genuine or pretended (while in fact both were acted for reasons of experimental control). We found stronger activations in regions associated with affect sharing (anterior insula [aIns] and anterior mid-cingulate cortex) as well as with affective self-other distinction (right supramarginal gyrus [rSMG]), in participants watching video clips of genuine vs. pretended facial expressions of pain. Using dynamic causal modeling, we then assessed the neural dynamics between the right aIns and rSMG in these two conditions. This revealed a reduced inhibitory effect on the aIns to rSMG connection for genuine pain compared to pretended pain. For genuine pain only, brain-to-behavior regression analyses highlighted a linkage between this inhibitory effect on the one hand, and pain ratings as well as empathic traits on the other. These findings imply that if the pain of others is genuine and thus calls for an appropriate empathic response, neural responses in the aIns indeed seem related to affect sharing and self-other distinction is engaged to avoid empathic over-arousal. In contrast, if others merely pretend to be in pain, the perceptual salience of their painful expression results in neural responses that are down-regulated to avoid inappropriate affect sharing and social support.
Comparative Effects of Energy‐Related Saline Wastewaters and Sodium Chloride on Hatching, Survival, and Fitness‐Associated Traits of Two Amphibian Species

Salinity (sodium chloride [NaCl]) is a prevalent and persistent contaminant that negatively affects freshwater ecosystems. Although most studies focus on effects of salinity from road salts (primarily NaCl), high‐salinity wastewaters from energy extraction (wastewaters) could be more harmful because they contain NaCl and other toxic components. Many amphibians are sensitive to salinity, and their eggs are thought to be the most sensitive life‐history stage. However, there are few investigations with salinity that include eggs and larvae sequentially in long‐term exposures. We investigated the relative effects of wastewaters from a large energy reserve, the Williston Basin (USA), and NaCl on northern leopard (Rana pipiens) and boreal chorus (Pseudacris maculata) frogs. We exposed eggs and tracked responses through larval stages (for 24 days). Wastewaters and NaCl caused similar reductions in hatching and larval survival, growth, development, and activity, while also increasing deformities. Chorus frog eggs and larvae were more sensitive to salinity than leopard frogs, suggesting species‐specific responses. Contrary to previous studies, eggs of both species were less sensitive to salinity than larvae. Our ecologically relevant exposures suggest that accumulating effects can reduce survival relative to starting experiments with unexposed larvae. Alternatively, egg casings of some species may provide some protection against salinity. Notably, effects of wastewaters on amphibians were predominantly due to NaCl rather than other components. Therefore, findings from studies with other sources of increased salinity (e.g., road salts) could guide management of wastewater‐contaminated ecosystems, and vice versa, to mitigate effects of salinization. Environ Toxicol Chem 2021;40:3137–3147. © 2021 SETAC
ADSORPTION KINETICS OF AN CATIONIC DYE FFROM AQUEOUSE SOLUTION BY ALGERIAN MINERAL MATERIALS

Abstract The objective of this study is to determine the power of two algerian mineral materials (kaolin and diatomite ) ,in order to remove an cationic dye(methylene blue )from aqueous solution ,materials have been characterized by different techniques . experiments have been studied on bath mode ,the influence of some parameters had been studied , such as , contact time ,the pH of solution and initial concentration . kinetics show that adsorption of methylene blue on to diatomite is faster than processus of adsorption on to kaoln ,and can be demonstrated by the pseudo-second –order model. Diatomite and kaolin used in this study improve their efficiency on elimination of methylene blue and could be low-cost alternative materials.
Mortality in Cancer Patients With COVID-19 Who Are Admitted to an ICU or Who Have Severe COVID-19: A Systematic Review and Meta-Analysis

PURPOSE There are scarce data to aid in prognostication of the outcome of critically ill cancer patients with COVID-19. In this systematic review and meta-analysis, we investigated the mortality of critically ill cancer patients with COVID-19. METHODS We searched online databases and manually searched for studies in English that reported on outcomes of adult cancer patients with COVID-19 admitted to an intensive care unit (ICU) or those with severe COVID-19 between December 2019 and October 2020. Risk of bias was assessed by the Modified Newcastle-Ottawa Scale. The primary outcome was all-cause mortality. We also determined the odds of death for cancer patients versus noncancer patients, as also outcomes by cancer subtypes, presence of recent anticancer therapy, and presence of one or more comorbidities. Random-effects modeling was used. RESULTS In 28 studies (1,276 patients), pooled mortality in cancer patients with COVID-19 admitted to an ICU was 60.2% (95% CI, 53.6 to 6.7; I2 = 80.27%), with four studies (7,259 patients) showing higher odds of dying in cancer versus noncancer patients (odds ratio 1.924; 95% CI, 1.596 to 2.320). In four studies (106 patients) of patients with cancer and severe COVID-19, pooled mortality was 59.4% (95% CI, –39.4 to 77.5; I2 = 72.28%); in one study, presence of hematologic malignancy was associated with significantly higher mortality compared with nonhematologic cancers (odds ratio 1.878; 95% CI, 1.171 to 3.012). Risk of bias was low. CONCLUSION Most studies were reported before the results of trials suggesting the benefit of dexamethasone and tocilizumab, potentially overestimating mortality. The observed mortality of 60% in cancer patients with COVID-19 admitted to the ICU is not prohibitively high, and admission to the ICU should be considered for selected patients (registered with PROSPERO, CRD42020207209).