Miracle-dz/UniFAR-FTUs huggingface dataset:
Human visual gamma for color stimuli

Strong gamma-band oscillations in primate early visual cortex can be induced by homogeneous color surfaces (Peter et al., 2019; Shirhatti and Ray, 2018). Compared to other hues, particularly strong gamma oscillations have been reported for red stimuli. However, precortical color processing and the resultant strength of input to V1 have often not been fully controlled for. Therefore, stronger responses to red might be due to differences in V1 input strength. We presented stimuli that had equal luminance and cone contrast levels in a color coordinate system based on responses of the lateral geniculate nucleus, the main input source for area V1. With these stimuli, we recorded magnetoencephalography in 30 human participants. We found gamma oscillations in early visual cortex which, contrary to previous reports, did not differ between red and green stimuli of equal L-M cone contrast. Notably, blue stimuli with contrast exclusively on the S-cone axis induced very weak gamma responses, as well as smaller event-related fields and poorer change-detection performance. The strength of human color gamma responses for stimuli on the L-M axis could be well explained by L-M cone contrast and did not show a clear red bias when L-M cone contrast was properly equalized.
Validation and reliability testing of the BREAST-Q expectations questionnaire in Swedish

Abstract Knowledge about preoperative expectations and how they affect satisfaction with breast reconstruction are key in improving care. A prerequisite for such studies are methodologically sound ways to define and measure expectations. The aims of this study were to translate and culturally adapt BREAST-Q Expectations for Sweden, and to perform a psychometric evaluation of the questionnaire. A cross-sectional study was performed. BREAST-Q expectations was translated according to current guidelines and sent to all patients on the waiting list for a breast reconstruction in our department. Internal consistency was assessed by Cronbach’s α. Inter-item correlations were calculated, and convergent validity was evaluated using a subjective comparator. Bland–Altman plots were drawn to evaluate test–retest reliability. Floor and ceiling effects were calculated. The questionnaire was sent to 198 patients, of which 129 responded (65%). Internal consistency was acceptable for all domains (Cronbach’s α 0.71–0.85) and all except one inter-item correlations were within the predefined intervals. Bland–Altman plots indicated that the agreement is variable. Ceiling effects were high for most domains. The results of the study support that the Swedish version of BREAST-Q expectations has a good content and face validity and internal consistency. Convergent validity and known-group validation cannot be adequately examined for expectations. Further studies are needed regarding test–retest reliability. High ceiling effects indicate that the instrument cannot discriminate between patients with high expectations and very high expectations. More studies are needed on how we can evaluate if expectations are realistic or not. Trial registration ClinicalTrials.gov Identifier NCT04714463, 19 January 2021 Patient and public contribution: All the data were generated directly from patients.
Mortality in cold agglutinin disease shows seasonal pattern

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Consumer emotional response as a predictor of preferences: A case of hotel style design

As the hospitality industry grows, so does the number of consumers booking hotels online. These consumers choose hotels based on webpage information such as hotel pictures and other promotional media. Given the importance of visual stimuli displayed on hotel webpages, little research effort has been devoted to the guests’ emotional response to hotel design. The aim of this study is to measure consumer’s emotional responses and how they can predict preferences in regards to hotel style design; the emotions we are referring to are those that are experienced by potential and current hotel customers and evoked by hotels. A within subjects experiment research design was utilized with a survey conducted from a convenience sample of 120 student respondents. Photo stimuli consisting of pictures from four different hotels was used to elicit emotional responses to hotel stimuli. A likert-type scale was utilized to measure the visual self-report of emotional responses and the corresponding hotel preferences. Results of the research show that hotel style design elicits emotional responses, and those emotional responses can be used to predict hotel preferences. Male respondents are more likely to report higher levels of negative emotional response than females, and nonNorwegian citizens are more likely to report higher levels of positive emotional response to hotel style design than Norwegians. These findings support previous literature regarding emotional response and preferences, and can be useful to hotel management to encourage measurement of their potential and current consumers’ emotional response to hotel style design in order to help predict the consumers’ preferences accordingly.
Panel sequencing links rare, likely damaging gene variants with distinct clinical phenotypes and outcomes in juvenile-onset SLE

Abstract Objectives Juvenile-onset systemic lupus erythematosus (jSLE) affects 15–20% of lupus patients. Clinical heterogeneity between racial groups, age groups and individual patients suggests variable pathophysiology. This study aimed to identify highly penetrant damaging mutations in genes associated with SLE/SLE-like disease in a large national cohort (UK JSLE Cohort Study) and compare demographic, clinical and laboratory features in patient sub-cohorts with ‘genetic’ SLE vs remaining SLE patients. Methods Based on a sequencing panel designed in 2018, target enrichment and next-generation sequencing were performed in 348 patients to identify damaging gene variants. Findings were integrated with demographic, clinical and treatment related datasets. Results Damaging gene variants were identified in ∼3.5% of jSLE patients. When compared with the remaining cohort, ‘genetic’ SLE affected younger children and more Black African/Caribbean patients. ‘Genetic’ SLE patients exhibited less organ involvement and damage, and neuropsychiatric involvement developed over time. Less aggressive first line treatment was chosen in ‘genetic’ SLE patients, but more second and third line agents were used. ‘Genetic’ SLE associated with anti-dsDNA antibody positivity at diagnosis and reduced ANA, anti-LA and anti-Sm antibody positivity at last visit. Conclusion Approximately 3.5% of jSLE patients present damaging gene variants associated with younger age at onset, and distinct clinical features. As less commonly observed after treatment induction, in ‘genetic’ SLE, autoantibody positivity may be the result of tissue damage and explain reduced immune complex-mediated renal and haematological involvement. Routine sequencing could allow for patient stratification, risk assessment and target-directed treatment, thereby increasing efficacy and reducing toxicity.
The Role of Novelty Satisfaction in Distance Physical Education During the COVID-19 Pandemic: A Self-Determination Theory Perspective

Purpose: To better understand the role of novelty satisfaction in distance physical education (PE) during the COVID-19 pandemic, this study aimed to determine the relationships among students’ novelty satisfaction, basic psychological needs satisfaction, autonomous motivation, effort, and enjoyment and to examine the mediating effects of autonomous motivation in the relationships between novelty satisfaction and effort and enjoyment. Methods: The participants were 332 undergraduate students from 10 PE classes. Structural equation modeling was used for data analysis. Results: Satisfaction of students’ need for novelty and the three basic needs positively predicted autonomous motivation, which in turn positively predicted effort and enjoyment. The relationships between students’ novelty satisfaction and their effort and enjoyment were mediated by autonomous motivation. Conclusion: This study provides important insights into needs satisfaction and motivational underpinnings of outcomes in distance PE during the COVID-19 pandemic. We suggest that future studies develop novelty support strategies based on our findings.
Dysbiosis in the Gut Microbiota in Patients with Inflammatory Bowel Disease during Remission

Dysbiosis in inflammatory bowel disease (IBD) has been implicated as a causal or contributory factor to the pathogenesis of the disease. This study, done on patients in remission while accounting for various confounding factors, shows significant community differences and altered community dynamics, even after acute inflammation has subsided. ABSTRACT Inflammatory bowel disease (IBD) is a chronic, relapsing, inflammatory disorder which comprises two main conditions: Crohn’s disease (CD) and ulcerative colitis (UC). Although the etiology of IBD has not been fully elucidated, the gut microbiota is hypothesized to play a vital role in its development. The aim of this cross-sectional study was to characterize the fecal microbiota in CD or UC patients in a state of remission to reveal potential factors sustaining residual levels of inflammation and triggering disease relapses. Ninety-eight IBD patients in a state of clinical remission (66 UC, 32 CD) and 97 controls were recruited, and stool samples, as well as detailed patient data, were collected. After DNA extraction, the variable regions V1 and V2 of the 16S rRNA gene were amplified and sequenced. Patients with IBD had a decrease in alpha diversity compared to that of healthy controls, and the beta diversity indices showed dissimilarity between the cohorts. Healthy controls were associated with the beneficial organisms unclassified Akkermansia species (Akkermansia uncl.), Oscillibacter uncl. , and Coprococcus uncl. , while flavonoid-degrading bacteria were associated with IBD. Network analysis identified highly central and influential disease markers and a strongly correlated network module of Enterobacteriaceae which was associated with IBD and could act as drivers for residual inflammatory processes sustaining and triggering IBD, even in a state of low disease activity. The microbiota in IBD patients is significantly different from that of healthy controls, even in a state of remission, which implicates the microbiota as an important driver of chronicity in IBD. IMPORTANCE Dysbiosis in inflammatory bowel disease (IBD) has been implicated as a causal or contributory factor to the pathogenesis of the disease. This study, done on patients in remission while accounting for various confounding factors, shows significant community differences and altered community dynamics, even after acute inflammation has subsided. A cluster of Enterobacteriaceae was linked with Crohn’s disease, suggesting that this cluster, which contains members known to disrupt colonization resistance and form biofilms, persists during quiescence and can lead to chronic inflammation. Flavonoid-degrading bacteria were also associated with IBD, raising the possibility that modification of dietary flavonoids might induce and maintain remission in IBD.
Staffing agency: A bridge to working during retirement

BACKGROUND: The ageing population has initiated a debate about a prolonged working life. There is an interest in finding the pre-retirement predictors of bridge employment and retirement decisions, but the understanding of the experiences of bridge employees is still limited. OBJECTIVES: The aim was to describe the characteristics of the pensioners working for a staffing agency, their motivational aspects, work patterns and types of services they provided. METHODS: This article analyses the results from a Swedish staffing agency’s yearly co-worker questionnaire from December 2017. The response rate was 44% (N = 1741). The design is descriptive, with correlation analyses and construction of typical cases. RESULTS: Most study participants were aged 65–74 years. Sixty-five percent were men, 66% were cohabitating/married, dominating education level was secondary school or higher education (79%). Important incentives for working were the social context and to gain extra income. A majority of the respondents also stated that their work increased their overall well-being. Seventy-eight percent worked 25 hours per month or less, 37% wanted to work more, 3% wanted to work less. Private services dominated with 61%; most common were gardening (43%), trades (33%) and cleaning (31%). CONCLUSIONS: More men than women chose this form of work. While single women need to work out of economic necessity, men, to a larger extent, work for the social context and well-being. The highest work frequency in 2017 (14%) in the population was found for those who retired in 2015, i.e. two years after their retirement year. A majority indicated that the work they were doing was different from earlier in their working life.
Characterization of the Serpentine Adeno-Associated Virus (SAAV) Capsid Structure: Receptor Interactions and Antigenicity

AAVs are widely studied therapeutic gene delivery vectors. However, preexisting antibodies and their detrimental effect on therapeutic efficacy are a primary challenge encountered during clinical trials. ABSTRACT Adeno-associated viruses (AAVs) are being developed as clinical gene therapy vectors. One issue undermining their broad use in the clinical setting is the high prevalence of circulating antibodies in the general population capable of neutralizing AAV vectors. Hence, there is a need for AAV vectors that can evade the preexisting immune response. One possible source of human naive vectors are AAVs that do not disseminate in the primate population, and one such example is serpentine AAV (SAAV). This study characterizes the structural and biophysical properties of the SAAV capsid and its receptor interactions and antigenicity. Single particle cryo-electron microscopy (cryo-EM) and thermal stability studies were conducted to characterize the SAAV capsid structure at pH 7.4, 6.0, 5.5, and 4.0, conditions experienced during cellular trafficking. Cell binding assays using Chinese hamster ovary (CHO) cell lines identified terminal sialic acid as the primary attachment receptor for SAAV similar to AAV1, 4, 5, and 6. The binding site of sialic acid to the SAAV capsid was mapped near the 2-fold axis toward the 2/5-fold wall, in a different location than AAV1, 4, 5, and 6. Towards determining the SAAV capsid antigenicity native immunodot blots showed that SAAV evades AAV serotype-specific mouse monoclonal antibodies. However, despite its reptilian origin, it was recognized by ~25% of 50 human sera tested, likely due to the presence of cross-reactive antibodies. These findings will inform future gene delivery applications using SAAV-based vectors and further aid the structural characterization and annotation of the repertoire of available AAV capsids. IMPORTANCE AAVs are widely studied therapeutic gene delivery vectors. However, preexisting antibodies and their detrimental effect on therapeutic efficacy are a primary challenge encountered during clinical trials. In order to circumvent preexisting neutralizing antibodies targeting mammalian AAV capsids, serpentine AAV (SAAV) was evaluated as a potential alternative to existing mammalian therapeutic vectors. The SAAV capsid was found to be thermostable at a wide range of environmental pH conditions, and its structure showed conservation of the core capsid topology but displays high structural variability on the surface. At the same time, it binds to a common receptor, sialic acid, that is also utilized by other AAVs already being utilized in gene therapy trials. Contrary to the initial hypothesis, SAAV capsids were recognized by one in four human sera tested, pointing to conserved amino acids around the 5-fold region as epitopes for cross-reacting antibodies.
Bridging the gap: Introducing a socio-cultural dimension to explain beliefs about man-made threats

The belief gap hypothesis focuses on why individuals differ in beliefs about the causes and consequences of issues despite expert consensus. Offering ideological rationalization and media use as an explanation for diverting beliefs, it, so far, has focused on ideological priors that describe traditional socio-economic cleavages—even if scientific and environmental issues go beyond monetary questions. In this study, we aim to counter this shortcoming by introducing a socio-cultural dimension of ideology to research on the belief gap hypothesis. Comparing two issues of man-made threats—climate change and antimicrobial resistance—and emphasizing more strongly the role of media use for belief gaps, we find that a socio-cultural dimension of ideology serves as a better predictor for diverting beliefs about climate change but not for antimicrobial resistance. In contrast to left-leaning media, using right-leaning media outlets widens climate change belief gaps.
Skimmin ameliorates cardiac function via the regulation of M2 macrophages in a myocardial infarction mouse model

Purpose Myocardial infarction (MI) is a coronary artery disorder with several complications, such as inflammation, oxidative stress, and cardiac fibrosis. The current study is aimed to explore the protective effect of skimmin (SKI) on impaired heart tissues in MI. Methods A mouse model of MI was induced by ligation of the left anterior descending artery. SKI was intragastric administration for 7 days after MI. Masson staining was then conducted to measure the area of fibrosis in the myocardium. The expression levels of collagen I and collagen III were analyzed using Western blot. The levels of glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and inflammatory factor were also detected. The expression of M1 polarization markers and M2 polarization markers in mice and LPS-induced RAW264.7 cells were detected using RT-qPCR and Western blot, respectively. Finally, the migration and proliferation of vascular smooth muscle cells (VSMCs) in vitro were analyzed using transwell and EDU, respectively. Results SKI improved cardiac function and cardiac fibrosis in mice with MI. SKI also decreased collagen I and collagen III expression, and inhibited inflammatory factor TNF-α, IL-1β, and IL-6 levels. SKI decreased the levels of MDA and increased the levels of GSH and SOD. Meanwhile, SKI could promote M2 macrophage polarization in vivo and in vitro. SKI could also repress the migration and proliferation of VSMCs. Conclusions SKI may ameliorate inflammation, oxidative stress, and cardiac fibrosis of MI by promoting M2 polarization.
Survival Characteristics and Transcriptomic Analyses Reveal the Adaptive Response of the Aquatic Pathogen Non-O1/O139 Vibrio cholerae to Starvation Stress

Non-O1/O139 V. cholerae is a causal agent of vibriosis that can be subject to nutrient insufficiency and cause high rates of mortality in aquatic animals. However, its molecular mechanisms of survival in response to starvation stress have been investigated only partially. ABSTRACT Non-O1/O139 Vibrio cholerae is a pathogen of various aquatic organisms but requires major self-regulation to overcome environmental stress in the aquatic environment. However, its survival strategies under environmental stress are not well understood. The objective of this study was to describe the survival characteristics and changes in expression of stress resistance-related genes of non-O1/O139 V. cholerae after 6 months of starvation at room temperature. The results demonstrated that starved cells were still viable, exhibited shortened rods and shrinking surface, and maintained virulence to Macrobrachium rosenbergii. To investigate the changes in gene expression in non-O1/O139 V. cholerae under starvation stress, especially those involved in stress resistance, transcriptome profiles of starved and wild-type cells were determined. The differentially expressed genes (DEGs) in starved cells were identified, including 191 upregulated genes and 180 downregulated genes. Among these DEGs, the well-known stress resistance-related genes were upregulated significantly, including rpoS, rpoD, rpoN, rpoE, uspA, uspC, cspD, hslJ, etc. Gene Ontology (GO) analysis of the DEGs demonstrated that environmental adaptation-related categories, such as response to stimulus and signal transduction, were upregulated significantly in the starved cells, while cell motility was downregulated significantly. These DEGs were also enriched into 54 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, including biofilm formation, two-component system, quorum sensing, flagellar assembly, bacterial chemotaxis stress resistance-related pathways, etc. The potential existence of long-starved non-O1/O139 V. cholerae bacteria in the aquatic environment may raise new concerns about this devastating pathogen in aquaculture. IMPORTANCE Non-O1/O139 V. cholerae is a causal agent of vibriosis that can be subject to nutrient insufficiency and cause high rates of mortality in aquatic animals. However, its molecular mechanisms of survival in response to starvation stress have been investigated only partially. Here, we demonstrate that under starvation stress, non-O1/O139 V. cholerae can survive over the long term and cause disease by dwarfing of the cell structure, upregulation of a series of stress resistance-related genes, and downregulation of flagellum assembly-related genes. This knowledge can help the development of intervention strategies to control non-O1/O139 V. cholerae infection in aquaculture.
Learning to Answer Visual Questions from Web Videos

Recent methods for visual question answering rely on large-scale annotated datasets. Manual annotation of questions and answers for videos, however, is tedious, expensive and prevents scalability. In this work, we propose to avoid manual annotation and generate a large-scale training dataset for video question answering making use of automatic cross-modal supervision. We leverage a question generation transformer trained on text data and use it to generate question-answer pairs from transcribed video narrations. Given narrated videos, we then automatically generate the HowToVQA69M dataset with 69M video-question-answer triplets. To handle the open vocabulary of diverse answers in this dataset, we propose a training procedure based on a contrastive loss between a video-question multi-modal transformer and an answer transformer. We introduce the zero-shot VideoQA task and the VideoQA feature probe evaluation setting and show excellent results. Furthermore, our method achieves competitive results on MSRVTT-QA, ActivityNet-QA, MSVD-QA and How2QA datasets. We also show that our approach generalizes to another source of web video and text data. We generate the WebVidVQA3M dataset from videos with alt-text annotations, and show its benefits for training VideoQA models. Finally, for a detailed evaluation we introduce iVQA, a new VideoQA dataset with reduced language bias and high-quality manual annotations.