Miracle-dz/UniFAR-FTUs huggingface dataset:
Betulinic acid accelerates diabetic wound healing by modulating hyperglycemia-induced oxidative stress, inflammation and glucose intolerance

Abstract Background Diabetes significantly delays wound healing through oxidative stress, inflammation and impaired re-epithelialization that lead to defective regulation of the healing process, although the related mechanism remains unclear. Here, we aim to investigate the potential role and mechanism for the beneficial effect of betulinic acid (BA) on diabetic wound healing. Methods The molecular effect of BA on hyperglycemia-mediated gene expression, oxidative stress, inflammation and glucose uptake was evaluated in endothelial, fibroblast and muscle cells. Burn injury was introduced to streptozotocin-induced diabetic rats and BA administration through either an intraperitoneal (IP) or topical (TOP) technique was used for wound treatment. Glucose tolerance was evaluated in both muscle tissue and fibroblasts, while oxidative stress and inflammation were determined in both the circulatory system and in wound tissues. The effect of BA on the wound healing process was also evaluated. Results BA treatment reversed hyperglycemia-induced glucose transporter type 4 (GLUT4) suppression in both muscle and fibroblast cells. This treatment also partly reversed hyperglycemia-mediated suppression of endothelial nitric oxide synthase (eNOS), nuclear factor erythroid 2-related factor 2 (Nrf2) signaling and nuclear factor NFκB p65 subunit (NFκB p65) activation in endothelial cells. An in vivo rat study showed that BA administration ameliorated diabetes-mediated glucose intolerance and partly attenuated diabetes-mediated oxidative stress and inflammation in both the circulatory system and wound tissues. BA administration by both IP and TOP techniques significantly accelerated diabetic wound healing, while BA administration by either IP or TOP methods alone had a significantly lower effect. Conclusions BA treatment ameliorates hyperglycemia-mediated glucose intolerance, endothelial dysfunction, oxidative stress and inflammation. Administration of BA by both IP and TOP techniques was found to significantly accelerate diabetic wound healing, indicating that BA could be a potential therapeutic candidate for diabetic wound healing.
Understanding Social Dimensions in Wildlife Conservation: Multiple Stakeholder Views

Simple Summary Garnering support from multiple stakeholders to increase the number or size of protected areas remains a key challenge for wildlife conservation efforts in Malaysia. Human–wildlife conflict often arises when local socio-economic development compromises wildlife survival due to negative landscape changes. It is essential to assess both human–wildlife conflict and human–human conflicts about wildlife, in order to promote mutually beneficial human–wildlife coexistence. This paper examines pertinent factors influencing wildlife conservation by integrating ecological and social approaches using a conservation planning framework. The findings demonstrate the importance of appraising social values to address issues such as size limits for protected areas and compensation for wildlife damage to property. It shows that monetary incentives are not the sole determinant in gaining the support of indigenous people in reporting wildlife crimes and their active participation in conservation programs. Therefore, developing effective communication with stakeholders, empowerment of rural communities, and proper appraisal of social values are all urgently needed to promote effective rural wildlife conservation programs. Abstract Numerous studies show the importance of social understanding in addressing multifaceted conservation issues. Building on a conservation planning framework, this study examines the social dimensions of wildlife conservation in Kinabatangan, Sabah, Malaysia. It employs a qualitative approach by conducting in-depth, semi-structured interviews with sixty informants drawn from local community members, government officials, tourism operators, non-government organizations, and the private sector. Our results show that the incidence of human–wildlife conflicts has reduced in the region, but that conflicts among stakeholders themselves about wildlife still remain a significant threat for attaining successful conservation outcomes. Further stakeholder perceptions of increased wildlife numbers often contrast with actual counts returned by periodical surveys conducted by conservation agencies, e.g., showing a 30% decline of orangutans and a 29% decline of gibbon abundance. This shows that evidence-based conservation messages have not been communicated well. The study has implications for enhancing social values among conservation players, promoting local community empowerment and revising conservation awareness programs.
Chemical Composition, Nutritive Value, Volatile Profiles and Antioxidant Activity of Coconut (Cocos nucifera L.) Haustorium with Different Transverse Diameter

In order to promote the development and utilization of coconut haustorium (CH). The basic chemical composition, volatile profiles and antioxidant activities of three haustoria with different transverse diameters were investigated. Results showed large coconut haustorium (LCH) contained more soluble sugar (47.10%) and reducing sugar (17.68%), while small coconut haustorium (SCH) possessed more ash (10.17%), protein (9.22%) and fat (5.03%). All CH were rich in potassium (4.06–4.69%) and phosphorus (0.39–0.50%). The fatty acid composition of SCH and amino acid composition of middle coconut haustorium (MCH) was more reasonable, which indicated its relatively higher nutritive value. Acids ranging from 26.90% to 60.82% were the dominant volatile components in CH, especially isobutyric acid whose relative content in SCH was up to 56.78%. The haustorium extract with polysaccharide as the main component has certain antioxidant activities, the half eliminating concentration (EC50 values) of LCH on hydroxyl radical and SCH on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical were 8.33, 1.18 and 2.44 mg/mL, respectively. These results provided a reference for the development and utilization of different CH as a raw material in functional food or dietary additives.
How Well Are Hand Hygiene Practices and Promotion Implemented in Sierra Leone? A Cross-Sectional Study in 13 Public Hospitals

Healthcare-associated infections (HAIs) result in millions of avoidable deaths or prolonged lengths of stay in hospitals and cause huge economic loss to health systems and communities. Primarily, HAIs spread through the hands of healthcare workers, so improving hand hygiene can reduce their spread. We evaluated hand hygiene practices and promotion across 13 public health hospitals (six secondary and seven tertiary hospitals) in the Western Area of Sierra Leone in a cross-sectional study using the WHO hand hygiene self-Assessment framework in May 2021. The mean score for all hospitals was 273 ± 46, indicating an intermediate level of hand hygiene. Nine hospitals achieved an intermediate level and four a basic level. More secondary hospitals 5 (83%) were at the intermediate level, compared to tertiary hospitals 4 (57%). Tertiary hospitals were poorly rated in the reminders in workplace and institutional safety climate domains but excelled in training and education. Lack of budgets to support hand hygiene implementation is a priority gap underlying this poor performance. These gaps hinder hand hygiene practice and promotion, contributing to the continued spread of HAIs. Enhancing the distribution of hand hygiene resources and encouraging an embedded culture of hand hygiene practice in hospitals will reduce HAIs.
Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols

Liquid platelet-rich fibrin (PRF) is produced by fractionation of blood without additives that initiate coagulation. Even though liquid PRF is frequently utilized as a natural source of fibrinogen to prepare sticky bone, the concentration of fibrinogen and the overall amount of “clottable PRF” components have not been evaluated. To this aim, we prepared liquid PRF at 300, 700, and 2000 relative centrifugal force (RCF), for 8 min and quantified the fibrinogen levels by immunoassay. We report here that, independent of the RCF, the fibrinogen concentration is higher in the platelet-poor plasma (PPP) compared to the buffy coat (BC) fraction of liquid PRF and further decreases in the remaining red fraction. We then determined the weight of the clotted PRF fractions before and after removing the serum. The PPP and BC fractions consist of 10.2% and 25.3% clottable matrix suggesting that more than half of the weight of clottable BC is caused by cellular components. Our data provide insights into the distribution of fibrinogen in the different fractions of liquid PRF. These findings suggest that PPP is the main source of clottable fibrinogen, while the BC is more a cell source when it comes to the preparation of sticky bone.
An accurate modeling approach to compute noise transfer gain in complex low power plane geometries of power converters

An approach based on a 2D lumped model is presented to quantify the voltage transfer gain (VTG) in power converter low power planes. The advantage of the modeling approach is the ease with which typical noise reduction devices such as decoupling capacitors or ferrite beads can be integrated into the model. This feature is enforced by a new modular approach based on effective matrix partitioning, which is presented in the paper. This partitioning is used to decouple power plane equations from external device impedance, which avoids the need for rewriting of a whole set of equation at every change. The model is quickly solved in the frequency domain, which is well suited for an automated layout optimization algorithm. Using frequency domain modeling also allows the integration of frequency-dependent devices such inductors and capacitors, which are required for realistic computation results. In order to check the precision of the modeling approach, VTGs for several layout configurations are computed and compared with experimental measurements based on scattering parameters.
Scale: Its Structure and The Fear of COVID-19 Scale: Its Structure and Measurement Invariance Across 48 Countries Its

The work of Artur Sawicki, Magdalena Żemojtel -Piotrowska, and Jarosław Piotrowski was supported by grant number 2017/26/E/HS6/00282 from the National Science Centre, Poland. Peter Jonason’s work was partially funded by the Polish National Agency for Academic Exchange (PPN/ULM/2019/1/00019/U/00001) and a grant from the National Science Centre of Poland (2019/35/B/HS6/00682). The authors declare that there was no direct collaboration between researchers from conflicting countries in accord with many national laws. Data, study materials, and all analyses codes are available at The study was a part of a preregistered project, which details are available at https://osf.io/hpwbj Contribution of each co-author in is pointed out in the brackets: Artur Sawicki (Conceptualization: analysis: Visualization: Writing Żemojtel (Conceptualization: Funding Project Writing Abstract COVID-19 has been a source of fear around the world. We asked whether the measurement of this fear is trustworthy and comparable across countries. In particular, we explored the measurement invariance and cross-cultural replicability of the widely-used Fear of COVID-19 scale (FCV-19S), testing community samples from 48 countries ( N = 14,558). The findings indicate that the FCV-19S has a somewhat problematic structure, yet the one-factor solution is replicable across cultural contexts and could be used in studies that compare people who vary on gender and educational level. The validity of the scale is supported by a consistent pattern of positive correlations with perceived stress and general anxiety. However, given the unclear structure of the FCV-19S, we recommend using latent factor scores, instead of raw scores, especially in cross-cultural comparisons.
Cotransplantation With Adipose Tissue–derived Stem Cells Improves Engraftment of Transplanted Hepatocytes

Background. Hepatocyte transplantation is expected to be an alternative therapy to liver transplantation; however, poor engraftment is a severe obstacle to be overcome. The adipose tissue–derived stem cells (ADSCs) are known to improve engraftment of transplanted pancreatic islets, which have many similarities to the hepatocytes. Therefore, we examined the effects and underlying mechanisms of ADSC cotransplantation on hepatocyte engraftment. Methods. Hepatocytes and ADSCs were cotransplanted into the renal subcapsular space and livers of syngeneic analbuminemic rats, and the serum albumin level was quantified to evaluate engraftment. Immunohistochemical staining and fluorescent staining to trace transplanted cells in the liver were also performed. To investigate the mechanisms, cocultured supernatants were analyzed by a multiplex assay and inhibition test using neutralizing antibodies for target factors. Results. Hepatocyte engraftment at both transplant sites was significantly improved by ADSC cotransplantation (P < 0.001, P < 0.001). In the renal subcapsular model, close proximity between hepatocytes and ADSCs was necessary to exert this effect. Unexpectedly, ≈50% of transplanted hepatocytes were attached by ADSCs in the liver. In an in vitro study, the hepatocyte function was significantly improved by ADSC coculture supernatant (P < 0.001). The multiplex assay and inhibition test demonstrated that hepatocyte growth factor, vascular endothelial growth factor, and interleukin-6 may be key factors for the abovementioned effects of ADSCs. Conclusions. The present study revealed that ADSC cotransplantation can improve the engraftment of transplanted hepatocytes. This effect may be based on crucial factors, such as hepatocyte growth factor, vascular endothelial growth factor, and interleukin-6, which are secreted by ADSCs.
The Revised Assessment of Sadistic Personality (ASP-8): Evidence for Validity across Four Countries

Abstract Subclinical sadism, characterized by infliction of cruelty, aggression, or humiliation on another for subjugation or pleasure, provides important information in the prediction of aversive behaviors that have implications for individuals’ and society’s well-being worldwide. Given sadism’s universal relevance, it is imperative that researchers ensure valid and reliable trait measurement not only among English-speaking individuals, but also cross-nationally among countries in which sadism remains relatively understudied. The objective of the current research was to validate the revised version of the Assessment of Sadistic Personality (ASP-8) (Plouffe et al., 2017) across samples of Russian (n = 1087, M age = 37.36, SD = 10.36), Greek (n = 1195, M age = 35.64, SD age = 13.08), Serbian (n = 443, M age = 28.10, SD age = 6.60), and British (n = 511, M age = 28.50, SD age = 11.62) adults. Overall, results supported the reliability, dimensionality, and scalar/partial scalar measurement invariance of the ASP-8 across cross-national samples. Convergent and discriminant validity were mostly supported through correlations with general personality traits, the Dark Triad, emotional intelligence, mental toughness, depression, anxiety, stress, satisfaction with life, aggression, and attitudes toward social groups. Based on our findings, we recommend the use of the ASP-8 in future investigations of aversive traits.
Distinct morphologies of arterial waveforms reveal preload‐, contractility‐, and afterload‐deficient hemodynamic instability: An in silico simulation study

Hemodynamic instability is frequently present in critically ill patients, primarily caused by a decreased preload, contractility, and/or afterload. We hypothesized that peripheral arterial blood pressure waveforms allow to differentiate between these underlying causes. In this in‐silico experimental study, a computational cardiovascular model was used to simulate hemodynamic instability by decreasing blood volume, left ventricular contractility or systemic vascular resistance, and additionally adaptive and compensatory mechanisms. From the arterial pressure waveforms, 45 features describing the morphology were discerned and a sensitivity analysis and principal component analysis were performed, to quantitatively investigate their discriminative power. During hemodynamic instability, the arterial waveform morphology changed distinctively, for example, the slope of the systolic upstroke having a sensitivity of 2.02 for reduced preload, 0.80 for reduced contractility, and −0.02 for reduced afterload. It was possible to differentiate between the three underlying causes based on the derived features, as demonstrated by the first two principal components explaining 99% of the variance in waveforms. The features with a high correlation coefficient (>0.25) to these principal components are describing the systolic up‐ and downstroke, and the anacrotic and dicrotic notches of the waveforms. In this study, characteristic peripheral arterial waveform morphologies were identified that allow differentiation between deficits in preload, contractility, and afterload causing hemodynamic instability. These findings are confined to an in silico simulation and warrant further experimental and clinical research in order to prove clinical usability in daily practice.
Connecting classroom English to real-world English : Taiwanese teachers' and students' perspectives on ELF-aware pedagogy

There has been considerable research on the global spread of English and its impact on English language use. In particular, there is extensive interest in how the fluid nature of the lingua franca use of English is impacting on real world communication and the consequent pedagogical implications for English language teaching (ELT) and acquisition. However, to date there has been limited research on the ways in which ELT teachers can adapt their teaching strategies to ensure that learners are equipped to use English in the real world. This study involves conducting a critical education inquiry using a Critical Pedagogy informed approach to observe Taiwanese teachers and undergraduate students of English, with the aims being to, firstly, identify the principles being employed by teachers and, secondly, to discover students’ understanding regarding learning English as a lingua franca (ELF) for real-world communication. To these ends, I posit the following research questions: 1. What learning/teaching theories and concepts inform Taiwanese teachers of English who teach for real-world communication? 2. What are students’ perceptions of learning English for the purposes of the real-world communication within the framework of Taiwanese English language education? I collected data from respondents in three universities and subsequent analysis revealed that the majority of classroom practice was geared towards teaching English as a foreign language rather than as a lingua franca that could be used in real life settings to communicate with a range of English speakers. Specifically, observation of classroom practice and interviews revealed that imitation and repetition strategies are commonly used to reproduce native English-speaking (NES) related linguacultural inputs, whereas critical, interactive, alternative and integrating approaches that are associated with lingua communication, although evident in some cases, were less engaged with. Albeit all of these strategies can serve a purpose in classroom teaching and learning, my evidence suggests that a rebalancing is needed, whereby teachers are more critical and flexible about the resources and approaches they use so as to ensure that these are suitable and context appropriate. In need of theoretical and practical support, teachers still endeavoured to explore ideas and activities for teaching and learning that were feasible in their instructional contexts in order to transcend the NES linguacultural dominance and provide lingua franca insights into classroom practices. Nevertheless, in most cases, students passively accepted the status quo. This highlighted the importance of students taking active roles in developing their own powers to be able to critically evaluate linguacultural resources and achieve balanced views on their own language acquisition and proficiency. This calls for opportunities to be created for students to legitimate their use of resources and skills to learn and use English on their own terms.
Characterizing Behaviors Associated with Enteric Pathogen Exposure among Infants in Rural Ecuador through Structured Observations

ABSTRACT. The relative importance of environmental pathways that results in enteropathogen transmission may vary by context. However, measurement of contact events between individuals and the environment remains a challenge, especially for infants and young children who may use their mouth and hands to explore their environment. Using a mixed-method approach, we combined 1) semistructured observations to characterize key behaviors associated with enteric pathogen exposure and 2) structured observations using Livetrak, a customized software application, to quantify the frequency and duration of contacts events among infants in rural Ecuador. After developing and iteratively piloting the structured observation instrument, we loaded the final list of prompts onto a LiveTrak pallet to assess environmental exposures of 6-month infants (N = 19) enrolled in a prospective cohort study of diarrheal disease. Here we provide a detailed account of the lessons learned. For example, in our field site, 1) most mothers reported washing their hands after diaper changes (14/18, 77.8%); however only a third (4/11, 36.4%) were observed washing their hands; 2) the observers noted that animal ownership differed from observed animal exposure because animals owned by neighboring households were reported during the observation; and 3) using Livetrak, we found that infants frequently mouthed their hands (median = 1.9 episodes/hour, median duration: 1.6 min) and mouthed surroundings objects (1.8 episodes/hour, 1.9 min). Structured observations that track events in real time, can complement environmental sampling, quantitative survey data and qualitative interviews. Customizing these observations enabled us to quantify enteric exposures most relevant to our rural Ecuadorian context.