Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression and role of HEPIS in breast cancer

Human embryo lung cellular protein interacting with severe acute respiratory syndrome-coronavirus nonstructural protein-10 (HEPIS) is expressed at varying levels in multiple organs and breast cancer cell lines. However, its expression and function in breast cancer cells has yet to be studied. Therefore, RNA in situ hybridization was used to detect the expression of HEPIS in breast cancer and cancer-adjacent normal breast tissue. HEPIS was expressed at lower levels in breast cancer compared with that in adjacent normal tissue. Subcellular localization of HEPIS was mainly found in the cytoplasm of HeLa cells. Cell Counting Kit-8 and 5-ethynyl-2′-deoxyuridine cell proliferation assays were used to investigate the role of HEPIS in cancer cell proliferation. Ectopic expression of HEPIS in MCF-7 cells was found to significantly inhibit cell proliferation. In contrast, knockdown of HEPIS by RNA interference exhibited the opposite effect. Furthermore, a dual-luciferase reporter assay was performed and HEPIS overexpression specifically inhibited the activity of the NF-κB reporter gene. Results of the gene chip assay revealed that 2,231 genes were differentially expressed in HEPIS-overexpressing cells. Results of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that these genes were enriched in the ‘mitogen-activated protein kinase signaling pathway’, ‘JAK-STAT signaling pathway’ and ‘focal adhesion’. Reverse transcription-quantitative PCR was used to confirm the expression levels of the differentially expressed genes interleukin 2 receptor subunit α (IL2RA), interferon α and β receptor subunit 2 (IFNAR2) and IFα8 (IFNA8). In conclusion, the results of the present study indicated that HEPIS may function as a potential repressor of breast cancer.
EFFECT OF COMBINATION OF NIGELLA SATIVA AND TRIGONELLA FOENUM-GRAECUM WITH GLIBENCLAMIDE ON SERUM TRIGLYCERIDE, HDL AND CREATININE LEVELS IN TYPE-2 DIABETES MELLITUS PATIENTS

To evaluate the effect of combination of Nigella sativa and Trigonella foenum-graecum seeds with Glibenclamide on serum triglyceride, HDL, and creatinine levels in type-2 diabetes mellitus patients. Diabetic outpatient clinics of Isra University Hyderabad and Liaquat University of Medical and Health Sciences Jamshoro, from March to August 2008. For this study Type-2 diabetic patients on Glibenclamide, who gave written consent to volunteer in the study, were randomly divided into two groups. Fifty patients in group A (Control Group) were maintained on routine dose of Glibenclamide, while fifty patients in group “B” (Intervention group) were given capsules containing equal amount of combined powder of N-sativa and T. foenum-graecum seeds, in addition to their routine dose of Glibenclamide. Serum triglyceride, HDL, and creatinine level were measured for every patient at the start and after 3 months of therapy. Comparison of the mean values of parameters measured in both the groups showed that serum HDL levels were significantly (P < 0.05) raised in intervention group as against the control, whereas the levels for serum triglycerides was comparable in both the groups. Results also showed that no significant change occurred in serum creatinine level in both the groups. This study provides evidence that the combination therapy of N. sativa and T. foenumgraecum with Glibenclamide is beneficial for type-2 diabetic patients as it increases serum HDL level in these patients.
Long-Lasting Memory Impairments after Subchronic Immune Challenge

Memory impairments and cognitive decline persist long after recovery from major illness or injury, and correlate with increased risk of later dementia. Here we developed a subchronic peripheral immune challenge model to examine delayed and persistent memory impairments in females and in males. We show that intermittent injections of either lipopolysaccharides or Poly I:C cause memory decline in both sexes that are evident eight weeks after the immune challenge. Importantly, we observed sex-specific patterns of deficits. Females showed impairments in object recognition one week after challenge that persisted for at least eight weeks. In contrast, males had intact memory one week after the immune challenge but exhibited broad impairments in memory tasks including object recognition, and both context and tone fear conditioning several months later. Together, these data suggest that subchronic immune challenge results in differential vulnerabilities of females and males to memory decline after immune challenge. This model will be an important tool for determining the mechanisms in both sexes that contribute to memory impairments that develop over the weeks and months after recovery from illness. Future studies using this model will provide new insights into the role of chronic inflammation in the pathogenesis of long-lasting memory decline and dementias.
Frequency of different types of paediatric ocular trauma attending a tertiary care paediatric ophthalmology department.

Objective: To assess the etiological factors and circumstances associated with the occurrence of eye injuries in children attending a tertiary care pediatric ophthalmology department. Methodology: This study was conducted at the Department of Pediatric Ophthalmology, Civil Hospital, Karachi from August 2007 to July 2009. Personal information, clinical findings and management plan was recorded on a prescribed performa. Variables studied included: age, sex, date of injury, place of injury, involved eye, circumstance and mechanism of injury, initial visual acuity and immediate management. Results: A total of 462 patients under the age of 16 years presented with ocular trauma. There were 297(64.3%) males and 165(35.7%) females. Mean age was 7.03 ± 3.61 years. Most cases 198 (42.9%) belonged to the schoolgoing age group (6 to 11years). The injuries occurred most frequently at home (215 patients, 46.5%). Blunt trauma occurred in 228(49.4%) cases followed by penetrating in 162(35.1%) cases. Injuries restricted to the eyelid occurred in 61 (13.2%) accidents, closed globe injury occurred in 338 (73.1%) and open globe injury in 63 (13.7%) accidents. Visual acuity was better than 6/12 in 245 (53.0%) children. Two hundred and fifteen children (46.5%) were prescribed only general measures like pressure patching, warm or cold compresses antibiotics or lubricant eye drops. Surgical management was required in 121(26.2%) patients. Conclusions: This study has shown frequencies of different type of ocular trauma which can be minimized by taking preventive measures as adopted in developed countries.
Use of Standardized Assessments and Online Resources in Stroke Rehabilitation

Background: The extent to which movement-related standardized assessments and online resources are used in stroke rehabilitation is unclear in the United States. Method: The researchers used a cross-sectional descriptive survey that examined (a) therapists use of movement-related standardized assessments, (b) factors influencing learning of new assessments, and (c) use of frequency of online resources by occupational therapists and physical therapists in the United States. Results: Of 151 respondents (46.4% occupational therapists, 53.6% physical therapists), the most frequently used movement-related assessments by occupational and physical therapists were the Berg and Fugl-Meyer Assessment, respectively. More physical therapists use motor-related standardized assessments regularly than occupational therapists, and physical therapists showed more consensus among standardized assessments. Both professions cited quality of patient care for motivating them to integrate outcome measures into practice. Most therapists in stroke rehabilitation used online resources to access movement-related standardized assessment content at least 25% of the time. The Rehabilitation Measures Database was the most frequently used website. Conclusion: Both occupational and physical therapists use online resources for movement-related standardized assessments on a regular basis. However, occupational therapists do not use standardized assessments as frequently as physical therapists. A systematic study of factors that impact the integration of standardized assessments is needed to further identify barriers and inform clinical practice change. Comments The authors report that they have no conflicts of interest to disclose.
A Clinical Study on Herpes Zoster During the Last 10-Year-Period (1994-2003)

Background: Herpes zoster is a common dermatologic disease characterized by unilateral pain and vesicular eruptions. The incidence of herpes zoster seems to be increasing recently. Objectives: The aim of this study was to elucidate the epidemiology and the clinical characteristics of herpes zoster. Method: During the 10-Year-Period from Jan. 1994 to Dec. 2003, 1089 patients with herpes zoster were evaluated in regard to annual incidence, age, sex, and monthly, seasonal, and dermatomic distributions. Patients with herpes zoster were further assessed concerning associated diseases and complications. Result: 1. The annual incidence averaged over the 10 years was 2.98% (1089 cases of total 36,531 outpatients) which is increasing recently. 2. Herpes zoster was seen most frequently in the 7th decade of life, and the ratio of male to female was 1:1.43. 3. There was no marked monthly or seasonal variation in the incidence of herpes zoster. 4. The most common dermatomic distribution was thoracic dermatome (49.6%), followed by trigeminal (19.5%), cervical (14.9%), lumbar (7.9%), sacral (5.3%) and multiple dermatomic involvement (2.8%). 5. Associated diseases of herpes zoster were observed in 398 patients (36.5%), which included hypertension (12.6%), diabetus mellitus(7.9%), gastric ulcer (2.8%), chronic renal failure (1.7%), asthma(1.7%), angina pectoris (1.6%), malignancy (0.6%) and so on. 6. The most common complication of herpes zoster was postherpetic neuralgia (7.4%), followed by eye complication (3.2%), secondary bacterial infection (1.8%), scar formation (0.8%), neurogenic bladder (0.4%), and Ramsay-Hunt syndrome (0.2%). Conclusion: Most of the results described in this study are similar to those previously reported, except for a higher incidence in female. The recent annual incidence of herpes zoster shows a tendency to increase.
SENTINEL LYMPH NODE BIOPSY IN BREAST CANCER BY USING ISOSULFAN BLUE

Objective: To determine the sensitivity of sentinel node involvement by breast cancer in case of clinically axillary lymph node negative breast cancer. Methodology: The study was conducted at Pakistan Institute of Medical Sciences, Islamabad in department of general surgery ward 5 from March 2006 to July 2007. Thirty patients with tumor size of either T1 or T2 and clinically negative axillary lymph node were enrolled in this study. These patients were subjected to sentinel lymph node biopsy by blue dye injected in peri-tumor parenchyma then dissection was done to localize sentinel node followed by formal axillary dissection (level II). The histopathology of sentinel node was compared with axillary lymph node. Results: Sentinel lymph node was successfully localized in all 30 patients (100%). The sensitivity, specificity, accuracy and false negative rate were 92.8%, 100%, 96.7%, 5.9% respectively. The axilla was metastatic in 14 cases (46.7%), one had negative sentinel lymph node but positive non sentinel lymph node (false negative), and four had sentinel lymph node metastasis alone. Conclusion: Sentinel lymph node biopsy by using blue dye have a high diagnostic accuracy and prevent unnecessary dissection of axilla in majority of patients with early node negative breast carcinoma.
Faktor Yang Berhubungan Dengan Keikutsertaan Ibu Hamil Dalam Kelas Ibu Hamil

Abstract Pregnant women class is a learning facility for pregnant women in undergoing the process of pregnancy and postpartum. Based on the preliminary survey conducted by researchers in the work area of ​​Payung Sekaki Primary Health Center, it was obtained data that the narration of midwives working in the work area of ​Payung Sekaki Primary Health Center was indeed carried out, but there were still many pregnant women who had not utilized maternal facilities optimally. The purpose of this study is the Factors Associated with the Participation of Pregnant Women in the Work Area Payung Sekaki Primary Health Center 2018. This type of research is quantitative using correlation analysis with Chi-square. The population in the study amounted to 297 people, with a total sample of 75 people with accidental sampling technique. This research was carried out in the work area of ​​Payung Sekaki Primary Health Center Pekanbaru on 05 to 28 July 2018. The results showed that there was a relationship between knowledge and maternal participation in the class of pregnant women in the work area of Payung Sekaki Primary Health Center with a p-value = 0,000, there was a relationship between parity with the participation of pregnant women in the Working Area of ​​Payung Sekaki Primary Health Center with a p-value = 0.025 and there is a relationship of family support with the participation of pregnant women in the work area of Payung Sekaki Primary Health Center with a p-value = 0.03 with a degree of Error α = 0,1. Keywords : Knowledge, parity, family support, participation of pregnant women Bibliography : 15 References (2007-2018).
Jack in the Duct: A Case of Common Biliary Duct Schwannoma

A 46-year-old woman was admitted with a history of painless, intermittent, self-resolving, cholestatic jaundice, without preceding prodrome for the past 1 year. She had 3 similar episodes without any significant past medical history. There was no history of significant weight loss, anorexia, hepatic decompensation, or any surgery in the past. Laboratory investigations showed a normal hemogram, conjugated hyperbilirubinemia with total bilirubin of 12 mg/dL and direct bilirubin of 9.6 mg/dL, normal aminotransferases, of 38 IU/L, elevated alkaline phosphatase (1,123 IU/L), and an albumin level of 3.4 g/dL with a gamma-glutamyl transferase (GGT) of 63 U/L. Hepatitis A, B, and C virus serology results were negative.
Bendamustine Plus Rituximab Versus CHOP Plus Rituximab in the First-Line-Treatment of Patients with Follicular, Indolent and Mantle Cell Lymphomas: Results of a Randomized Phase III Study of the Study Group Indolent Lymphomas (StiL)

Background: Promising results have been observed in our previous phase-II study evaluating the combination of Bendamustine plus Rituximab (B-R) in patients with relapsed/refractory indolent or mantle cell lymphomas. An overall response rate (ORR) of 90%, including a 60% rate of complete remissions (CR) was documented. Objective: In October 2003, we initiated a multicenter randomized phase-III study to compare efficacy and safety of the combination B-R versus CHOP plus Rituximab (CHOP-R) as first-line therapy for follicular, indolent and mantle cell lymphomas. Methods: Patients (pts) were randomized to receive Rituximab 375 mg/qm (day 1) plus either Bendamustine 90 mg/qm (days 1+2) every 28 days or the standard CHOP regimen every 21 days for a maximum of 6 cycles. The primary endpoint was event-free survival (EFS). The trial was calculated to power the study to demonstrate a non-inferior EFS associated with B-R treatment, as defined by a difference in EFS between the two regimes of less than 10% after 3 years. An event was defined by a response less than a partial response, disease progression, relapse, or death from any cause. The study is closed according to the planned recruitment schedule. Results: 546 patients have been randomized. For this second interim analysis, 437 patients are evaluable for response (B-R: n=221; CHOP-R: n=212). Median patient age is 64 years. Histologies are equally distributed between arms: follicular 52%, mantle cell 20%, and other indolent lymphomas 28% in both treatment groups, each. The ORR for pts treated with B-R was similar to that associated with CHOP-R (94% vs 93%, respectively). CR was also similar at 41% for B-R compared to 33% for CHOP-R. The median follow-up time for both groups is 28 months. Thus far, 50 deaths have been observed (B-R: 25; CHOP-R: 25). Progressive or relapsed disease has been documented during the follow-up period: 58 in pts treated with B-R and 75 in the CHOP-R group. The median EFS for B-R is not yet reached, the median EFS for CHOP-R is 39 months with no statistical significant difference for the EFS between both groups. The B-R regimen appears to have a better toxicity profile, as evidenced by a lower rate of total alopecia (0% with B-R vs. 89% CHOP-R) and a lower number of infectious complications (number of patients with infections of any grade were 56 (25%) in the B-R group vs. 78 (37%) in CHOP-R group). Correlating, the CHOP-R regimen was more hematotoxic: WHO grade 3/4 leukocytopenia was reported in 36% CHOP-R treated pts compared with 19% in pts treated with B-R, while in the CHOP-R group more frequently G-CSF was used. Conclusions: In this second interim analysis, the combination of Bendamustine plus Rituximab appears to be non-inferior to the standard CHOP-R while showing a better tolerability profile. Further updated results will be presented at this time.
Acute myocardial infarction in an adolescent female with normal coronary arteries.

Acute myocardial infarction may occur in young people with angiographically normal coronary arteries but the pathophysiology of this condition remains unknown. The possible mechanisms underlying myocardial infarction with normal coronary arteries are coronary vasospasm, thrombosis, embolization or minimal atherosclerosis. Smoking is an important predisposing risk factor for myocardial infarction in the presence of normal coronary arteries. We describe a case of acute myocardial infarction in a adolescent female who had normal coronary arteries according to multislice computed tomography coronary angiography results.
Non-invasive high flow oscillatory ventilation in comparison with nasal continuous positive pressure ventilation for respiratory distress syndrome, a literature review

Abstract Background Noninvasive high-frequency oscillatory ventilation (NHFOV) keeps the lung open with add-on effective rhythmic oscillations in addition to allowing spontaneous breathing. This review aims at reconstructing the different pieces of available research articles and evidence into a more solid collective evidence for NHFOV in preterm infants with respiratory distress syndrome (RDS). Methods A thorough systemic search was conducted in Medline, Embase, Web of Science, Google Scholar, CINAHL, and Cochrane. Randomized controlled trials (RCTs) on preterm infants with RDS comparing NHFOV with nasal continuous positive airway pressure (NCPAP) in terms of PCO2 change, need for ventilation, duration of respiratory support, mortality air leak, or BPD were included. Data quality assessment and meta-analyses were carried out. Results Five RCTs involving 270 preterm infants included in the review. PCO2 relatively decreased on NHFOV (MD = 3.84, confidence interval (CI) 7.32–0.35, p = . 03). On the other hand, relative risk (RR) of intubation was unquestionably decreased with NHFOV in comparison with NCPAP (RR = 0.43, CI 0.25–0.75, p = .003) without statistical heterogeneity I 2 = 0%. Although the risk of mortality was less in NHFOV, the difference was statistically insignificant (RR = 0.72, CI 0.24–2.18, p = .56). Other outcomes reported in single studies only. Duration of respiratory support was significantly shorter in NHFOV compared with NCPAP (37.35 ± 8.96 versus 49.77 ± 10.33, p = .009), whereas air leak and BPD were reported in very few cases without a significant difference between the two interventions. Conclusions NHFOV improved the PCO2 elimination and decreased the risk of intubation without a significant change in mortality compared with NCPAP.
Economic Burden of Treatment‐Resistant Depression Among Patients Hospitalized for Major Depressive Disorder in the United States

Objectives: This study aimed to evaluate hospital length of stay (LOS) and cost as well as readmission risk and the associated economic burden among patients hospitalized for treatment‐resistant and non–treatment‐resistant major depressive disorder. Methods: Adult patients with a primary hospital discharge diagnosis of major depressive disorder were identified from the Premier Hospital Database (January 1, 2012–September 30, 2015). Patients were stratified into two cohorts: those whose hospital treatment was suggestive of treatment‐resistant depression and those with non–treatment‐resistant depression. Hospital LOS and cost during the initial admission and readmissions rates, LOS, and cost within the 6‐month follow‐up were compared between cohorts with a propensity score–matched sample. Results: After matching, 45,066 patients were included in each cohort. For index hospitalizations, mean hospital LOS was longer (7.4 vs. 5.9 days, p<0.001) and mean hospital cost higher ($8,681 vs. $6,632, p<0.001) for patients with treatment‐resistant depression vs. non–treatment‐resistant depression. Rates for all‐cause (24.4% vs. 20.0%, p<0.001), major depressive disorder–related (17.0% vs. 13.3%, p<0.001), and suicidal ideation/suicide attempt–related (12.8% vs. 9.5%, p<0.001) readmissions were higher for patients with treatment‐resistant depression. Mean LOS and total hospital costs per patient for readmissions were also greater for patients with treatment‐resistant depression vs. non–treatment‐resistant depression. Correspondingly, the combined hospital cost (index hospitalization+all‐cause readmissions) was greater for patients with treatment‐resistant depression ($12,370 vs. $9,429, p<0.001). Conclusions: Treatment‐resistant depression was associated with substantial economic burden among patients hospitalized for major depressive disorder. More‐effective treatment and care for this patient population may reduce the hospital burden of patients with treatment‐resistant depression.
The Effect of Vitamin E on Cisplatin Induced Nephrotoxicity: A Clinical Trial Study

Background Cisplatin is a common platinum based chemotherapy drug that is commonly used for the treatment of the solid tumors. It is believed that this drug has nephrotoxic effects due to its oxidative action. Therefore, it is believed antioxidant agents such as vitamin E can prevent this nephrotoxic effect; however, the human studies are not sufficient. We aimedto assess the effect of vitamin E against cisplatin induced nephrotoxicity. Materials and Methods In a randomized controlled trial, the patients were grouped into two control and target group. Both groups should receive cisplatin with a dose of 50 mg/m2 as single dose or in 3 to 5 divided doses. The target group received 400 IU of vitamin E daily until two days after discontinuing cisplatin and the control group received only cisplatin. Patients’ serum urea, creatinine, and Kidney injury molecule-1 (KIM-1) levels were measured and compared between the two study groups and in a before-after manner. Results Totally, 29 patients were grouped into 17 controls and 12 patients in target group. The KIM-1biomarker was statistically higher in control group at the end of study (p=0.040). When assessing the before-after results, KIM-1 biomarker showed a significant decrease (1.10±0.32 pg/mL to 0.71±0.09 pg/mL; p<0.05). Conclusion Based on the results,it seems vitamin E can help to protect kidney against cisplatin toxicity; however, further clinical trials are needed to support our findings.