Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of Six Phenotypic Assays with Reference Methods for Assessing Colistin Resistance in Clinical Isolates of Carbapenemase-Producing Enterobacterales: Challenges and Opportunities

The global escalation of severe infections due to carbapenemase-producing Enterobacterales (CPE) isolates has prompted increased usage of parenteral colistin. Considering the reported difficulties in assessing their susceptibility to colistin, the purpose of the study was to perform a comparative evaluation of six phenotypic assays—the colistin broth disc elution (CBDE), Vitek 2 Compact (bioMérieux SA, Marcy l’Etoile, France), the Micronaut MIC-Strip Colistin (Merlin Diagnostika GMBH, Bornheim-Hensel, Germany), the gradient diffusion strip Etest (bioMérieux SA, Marcy l’Etoile, France), ChromID Colistin R Agar (COLR) (bioMérieux SA, Marcy l’Etoile, France), and the Rapid Polymyxin NP Test (ELITechGroup, Signes, France)—versus the reference method of broth microdilution (BMD). All false resistance results were further assessed using population analysis profiling (PAP). Ninety-two nonrepetitive clinical CPE strains collected from two hospitals were evaluated. The BMD confirmed 36 (39.13%) isolates susceptible to colistin. According to the BMD, the Micronaut MIC-Strip Colistin, the CBDE, and the COLR medium exhibited category agreement (CA) of 100%. In comparison with the BMD, the highest very major discrepancy (VMD) was noted for Etest (n = 15), and the only false resistance results were recorded for the Rapid Polymyxin NP Test (n = 3). Only the PAP method and the Rapid Polymyxin NP Test were able to detect heteroresistant isolates (n = 2). Thus, there is an urgent need to further optimize the diagnosis strategies for colistin resistance.
A New Combined Air Quality and Heat Index in Relation to Mortality in Monterrey, Mexico

The negative synergistic effects of air pollution and sensible heat on public health have been noted in numerous studies. While separate, simplified, and public-facing indices have been developed to communicate the risks of unhealthful levels of air pollution and extreme heat, a combined index containing elements of both has rarely been investigated. Utilizing air quality, meteorology, and mortality data in Monterrey, Mexico, we investigated whether the association between the air quality index (AQI) and mortality was improved by considering elements of the heat index (HI). We created combined indices featuring additive, multiplicative, and either/or formulations and evaluated their relationship to mortality. Results showed increased associations with mortality for models employing indices that combined the AQI and the HI in an additive or multiplicative manner, with increases in the interquartile relative risk of 3–5% over that resulting from models employing the AQI alone.
A critical evaluation of mobile guided tour apps: Motivators and inhibitors for tour guides and customers

This paper identifies the motivators and inhibitors for using mobile tour guiding apps from the perspective of tour guides and customers. The study provides insights into the challenges of designing and developing apps through the evaluation of an existing tour guiding app and makes recommendations for further improvement. In-depth interviews were undertaken with tour guides from the WeGoTrip App as well as a sample of their customers. This study has important implications for app designers in terms of usability of such apps. It provides insights into the readiness of guides to adopt and promote such new developments, as well as the experiences of potential customers. The paper contributes to technology acceptance literature in the under-researched area of mobile app technology acceptance. The focus is on the motivators/inhibitors of guides and tourists to use/re-use mobile apps in the context of guided tours.
Innovative financing of public infrastructure in Zimbabwe: Status vs. potential

The paper examines the status and future potential of innovative finance in mitigating public infrastructure financing gaps in Zimbabwe. The study is descriptive. Data were collected through 23 interviews and 32 questionnaires. Interviews were conducted with managers of government of Zimbabwe ministries and parastatal enterprises, and the results were analyzed using thematic analysis. Whilst the questionnaires were distributed to officers of government of Zimbabwe ministries and parastatal enterprises and analyzed using Stata v14. The findings revealed that Zimbabwe does not currently finance public infrastructure using conventional innovative financing instruments. However, there are innovations in the combination of conventional financing instruments such as bonds, loans, and budget appropriations to finance power (electricity) infrastructure to a limited extent. Scope and potential exist for using innovative finance once a supportive legal and regulatory framework for public private partnerships (PPP) and other innovative financing instruments is in place in Zimbabwe. Using a binary logistic regression model, the findings showed that the infrastructure sector is the only factor significantly influencing innovative infrastructure financing at the 5% significance level with p-value &lt; 0.05. The study recommends Zimbabwe to follow the South African Public Private Partnership framework by developing provincial and municipal regulations anchored in national legislation. There is latent potential for closing the public infrastructure financing gap in Zimbabwe using innovative finance.
Sex differences in perceived discomfort during seated static posture holding

Abstract This study investigated how sex modifies postural discomfort perception during a sagittally-symmetric, seated static posture holding (SPH) task. Ten male and 10 female participants performed SPH and conducted subjective discomfort ratings in a total of 108 task conditions. A regression analysis found that the impacts of the body joint reactive moments on perceived discomfort were larger for the female group than the male whereas that of the shoulder joint angle was more pronounced for the male than the female. Also, some of the 108 task conditions were found to be more uncomfortable for the male group, while some others, for the female. The observed sex impacts are thought to be due to the sex differences in physical work capacities (muscular strength and joint flexibility). The results suggest that new posture analysis tools allowing sex-specific analyses are needed as they would improve the accuracy and precision of ergonomics posture analyses. Practitioner summary: This study empirically investigated how sex modifies postural discomfort perception during a seated posture holding (SPH) task. Sex was found to modify the impacts of joint reactive moments and the shoulder joint angle. The study results seem to reflect the sex differences in muscular strength and joint flexibility.
Immediate direct-to-implant breast reconstruction with prepectoral vs. subpectoral approach: a narrative review

Department of Advanced Biomedical Sciences, University of Naples “Federico II”, Naples, Italy; Department of Clinical Medicine and Surgery, University of Naples “Federico II”, Naples, Italy Contributions: (I) Conception and design: N Velotti, N Rocco, A Vitiello, M Musella, S Masone; (II) Administrative support: N Velotti, N Rocco, A Vitiello, M Musella, S Masone; (III) Provision of study materials or patients: N Velotti, G Berardi, M Pontillo; (IV) Collection and assembly of data: N Velotti, N Rocco, A Vitiello, G Berardi, M Pontillo; (V) Data analysis and interpretation: N Velotti, N Rocco, A Vitiello, M Musella, S Masone; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Nunzio Velotti. Department of Advanced Biomedical Sciences, University of Naples “Federico II”, Via Pansini 5, 80131 Naples, Italy. Email: nunzio.velotti@gmail.com.
Elaboration of an organic beverage based on grape juice with positive nutritional properties

Abstract The present study aimed to develop a natural beverage with interesting phytochemical composition and biological activity based on grape juice without added sugars or artificial additives. Two groups of blends were made by diluting concentrate grape juice with a sugar content of 65 °Brix with two different mineral waters (BA: Bezoya with low mineralization; BB: Solan de Cabras with high mineralization). Lemon juice was used for pH correction, and mixtures of extractions of hop with tea and hop with mint were used to increase aroma. Samples were stored under refrigeration (4°C) then subjected to physicochemical and sensory analysis. The results demonstrated that malvidin‐3‐O‐glucoside pigment was the predominant pigment with a concentration ranging from 75.71 ± 12.49 to 84.87 ± 1.70 mg/L. The levels of sugars ranged from 79.90 ± 1.37 to 82.37 ± 0.55 g/L and total soluble solids were between 5.47 ± 0.12 and 5.77 ± 0.06 °Brix. Total acids presented a significant difference, ranging from 1.40 ± 0.00 to 1.43 ± 0.06 g/L in BA samples and from 1.10 ± 0.10 to 1.20 ± 0.00 g/L in BB samples. For 20 days, the color increased in all beverages. However, BA drinks presented higher acidity and higher red color intensity than BB drinks, so the type of water and pH influenced the color of beverages. The sensory evaluation showed that the beverage made with low mineral water and flavored with a mixture of hop with tea was preferred.
Blood Pressure and Heart Rate Responses to an Isokinetic Testing Protocol in Professional Soccer Players

The aim of this study was to determine blood pressure (BP) and heart rate (HR) responses triggered during an isokinetic testing protocol in professional soccer players and compare cardiovascular parameters at completion of this isokinetic protocol with those during a treadmill test. Using purposive sampling, 63 professional soccer players were recruited. Cardiovascular responses were measured noninvasively during a bilateral testing protocol of knee flexion and extension. Treadmill ergospirometry following an incremental speed protocol was performed to analyze the same cardiovascular parameters at rest and at completion of this test. There were significant differences in diastolic blood pressure (DBP) and HR according to field position. The parameters presented high homogeneity at both competitive levels. Systolic blood pressure, mean arterial pressure, HR, and rate pressure product at completion of the treadmill test were significantly higher than those at completion of the isokinetic protocol. Intermittent isokinetic testing protocol of the knee triggers normal and safe BP and HR responses in healthy professional soccer players. The HR of the defenders was higher than those of the forwards and midfielders but was independent of the competitive level. The values of cardiovascular parameters at isokinetic protocol completion were lower than those during the treadmill test.
Characteristics and Outcomes of COVID-19-Related Hospitalization among PLWH

Background: There is conflicting evidence for how HIV influences COVID-19 infection. The aim of this study was to compare characteristics at presentation and the clinical outcomes of people living with HIV (PLWH) versus HIV-negative patients (non-PLWH) hospitalized with COVID-19. Methods: Primary endpoint: time until invasive ventilation/death. Secondary endpoints: time until ventilation/death, time until symptoms resolution. Results: A total of 1647 hospitalized patients were included (43 (2.6%) PLWH, 1604 non-PLWH). PLWH were younger (55 vs. 61 years) and less likely to be with PaO2/FiO2 < 300 mmHg compared with non-PLWH. Among PLWH, nadir of CD4 was 185 (75–322) cells/μL; CD4 at COVID-19 diagnosis was 272 cells/μL (127–468) and 77% of these were virologically suppressed. The cumulative probability of invasive mechanical ventilation/death at day 15 was 4.7% (95%CI 1.2–17.3) in PLWH versus 18.9% (16.9–21.1) in non-PLWH (p = 0.023). The cumulative probability of non-invasive/invasive ventilation/death at day 15 was 20.9% (11.5–36.4) in PLWH versus 37.6% (35.1–40.2) in non-PLWH (p = 0.044). The adjusted hazard ratio (aHR) of invasive mechanical ventilation/death of PLWH was 0.49 (95% CI 0.12–1.96, p = 0.310) versus non-PLWH; similarly, aHR of non-invasive/invasive ventilation/death of PLWH was 1.03 (95% CI 0.53–2.00, p = 0.926). Conclusion: A less-severe presentation of COVID-19 at hospitalization was observed in PLWH compared to non-PLWH; no difference in clinical outcomes could be detected.
Neurons with Cat’s Eyes: A Synthetic Strain of α-Synuclein Fibrils Seeding Neuronal Intranuclear Inclusions

The distinct neuropathological features of the different α-Synucleinopathies, as well as the diversity of the α-Synuclein (α-Syn) intracellular inclusion bodies observed in post mortem brain sections, are thought to reflect the strain diversity characterizing invasive α-Syn amyloids. However, this “one strain, one disease” view is still hypothetical, and to date, a possible disease-specific contribution of non-amyloid factors has not been ruled out. In Multiple System Atrophy (MSA), the buildup of α-Syn inclusions in oligodendrocytes seems to result from the terminal storage of α-Syn amyloid aggregates first pre-assembled in neurons. This assembly occurs at the level of neuronal cytoplasmic inclusions, and even earlier, within neuronal intranuclear inclusions (NIIs). Intriguingly, α-Syn NIIs are never observed in α-Synucleinopathies other than MSA, suggesting that these inclusions originate (i) from the unique molecular properties of the α-Syn fibril strains encountered in this disease, or alternatively, (ii) from other factors specifically dysregulated in MSA and driving the intranuclear fibrillization of α-Syn. We report the isolation and structural characterization of a synthetic human α-Syn fibril strain uniquely capable of seeding α-Syn fibrillization inside the nuclear compartment. In primary mouse cortical neurons, this strain provokes the buildup of NIIs with a remarkable morphology reminiscent of cat’s eye marbles (see video abstract). These α-Syn inclusions form giant patterns made of one, two, or three lentiform beams that span the whole intranuclear volume, pushing apart the chromatin. The input fibrils are no longer detectable inside the NIIs, where they become dominated by the aggregation of endogenous α-Syn. In addition to its phosphorylation at S129, α-Syn forming the NIIs acquires an epitope antibody reactivity profile that indicates its organization into fibrils, and is associated with the classical markers of α-Syn pathology p62 and ubiquitin. NIIs are also observed in vivo after intracerebral injection of the fibril strain in mice. Our data thus show that the ability to seed NIIs is a strain property that is integrally encoded in the fibril supramolecular architecture. Upstream alterations of cellular mechanisms are not required. In contrast to the lentiform TDP-43 NIIs, which are observed in certain frontotemporal dementias and which are conditional upon GRN or VCP mutations, our data support the hypothesis that the presence of α-Syn NIIs in MSA is instead purely amyloid-strain-dependent.
Collateral Quality and House Prices†

This paper studies the effects of shifts in collateral quality on house prices and the macroeconomy in a dynamic general equilibrium model with credit and housing collateral. Depending on whether information on collateral quality is produced, our model features endogenous regime switching between two lending regimes. Shocks to collateral quality and the associated regime switching effectively amplify movements in house prices relative to rents and are able to reconcile the joint dynamics of house prices, the price-rent ratio and output observed in the data. Our estimation shows that the collateral quality shocks account for approximately half of variations in house prices and the price-rent ratio and a fifth of variations in investment during the housing boom and the Great Recession. *We thank Pengfei Wang, David Cook, Juanyi (Jenny) Xu, Yang Lu, Zhiwei Xu and Feng Dong for helpful discussions. We have also benefited from comments from seminar and conference participants at Hong Kong University of Science and Technology, Tsinghua University, Shanghai Jiao Tong University, Fudan University, Shanghai University of Finance and Economics, Southwestern University of Finance and Economics, the 2021 China Meeting of the Econometric Society, the 47th Money, Macro and Finance Research Group Annual Conference, and the 13th Joint Economics Symposium of Six Leading East Asian Universities. Zhou acknowledges financial support from Shanghai Institute of International Finance and Economics. All errors are our own. †Department of Economics, Fudan University, 600 Guoquan Road, Shanghai, China 200433. Tel. : (+86)21-64652989. Email: jingzhou@fudan.edu. cn.
Skydiver: A Spiking Neural Network Accelerator Exploiting Spatio-Temporal Workload Balance

Spiking neural networks (SNNs) are developed as a promising alternative to artificial neural networks (ANNs) due to their more realistic brain-inspired computing models. SNNs have sparse neuron firing over time, i.e., spatio-temporal sparsity; thus, they are useful to enable energy-efficient hardware inference. However, exploiting spatio-temporal sparsity of SNNs in hardware leads to unpredictable and unbalanced workloads, degrading the energy efficiency. In this work, we propose an FPGA-based convolutional SNN accelerator called Skydiver that exploits spatio-temporal workload balance. We propose the approximate proportional relation construction (APRC) method that can predict the relative workload channel-wisely and a channel-balanced workload schedule (CBWS) method to increase the hardware workload balance ratio to over 90%. Skydiver was implemented on a Xilinx XC7Z045 FPGA and verified on image segmentation and MNIST classification tasks. Results show improved throughput by <inline-formula> <tex-math notation="LaTeX">$1.4\times $ </tex-math></inline-formula> and <inline-formula> <tex-math notation="LaTeX">$1.2\times $ </tex-math></inline-formula> for the two tasks. Skydiver achieved 22.6KFPS throughput, and <inline-formula> <tex-math notation="LaTeX">$42.4~\mu \text{J}$ </tex-math></inline-formula>/image prediction energy on the classification task with 98.5% accuracy.
DOE/EPRI Electricity Storage Handbook in Collaboration with NRECA

The Electricity Storage Handbook (Handbook) is a how-to guide for utility and rural cooperative engineers, planners, and decision makers to plan and implement energy storage projects. The Handbook also serves as an information resource for investors and venture capitalists, providing the latest developments in technologies and tools to guide their evaluations of energy storage opportunities. It includes a comprehensive database of the cost of current storage systems in a wide variety of electric utility and customer services, along with interconnection schematics. A list of significant past and present energy storage projects is provided for a practical perspective. This Handbook, jointly sponsored by the U.S. Department of Energy and the Electric Power Research Institute in collaboration with the National Rural Electric Cooperative Association, is published in electronic form at www.sandia. gov/ess. This Handbook is best viewed online. iii Rev. 1, February 2015 DOE/EPRI Electricity Storage Handbook in Collaboration with NRECA Revision Log Comments, inquiries, corrections, and suggestions can be submitted via the website www.sandia. gov/ess/, beginning August 1, 2013.
ANTIBACDUS-PAN: Antibacterial Utilization among Adult Patients Before and During COVID-19 Pandemic within 12-Months Period: A Tertiary Hospital Pharmacoepidemiology Study

Objective: Irrational use of antibacterials is a concern during the COVID-19 pandemic. Hospital pharmacoepidemiology studies are important for evaluating the rational use of medicines, especially antibacterials, during pandemics. Defined daily doses (DDD) and drug utilization 90% (DU90%) are established methods for the evaluation of drug utilization. We aimed to evaluate antibacterial utilization in a tertiary hospital setting at Koç University Hospital (KUH). Materials and Methods: This cross-sectional, descriptive study was retrospectively conducted with data extracted from KUH Inpatient Electronic Order System (CP) and was carried out for a period of one year. Antibacterial utilization of adult (aged ≥ 18 years) inpatients, who were prescribed at least one type of systemic antibacterial (ATC code J01), was evaluated using the recommended parameter DDD/100 admission and compared between 6 months before COVID-19 and during COVID-19 periods. March 11, 2020, the very first COVID-19 diagnosed case in Turkey, was set as the cutoff date of the 6-month period for the selection of the compared antibacterials using the DU90% method. Results: Finally, 3280 of 5942 and 2605 of 4942 prescriptions for pre-COVID-19 and COVID-19 periods were included, respectively. Antibacterial utilization according to DDD/100 admissions increased from 193.96 to 201.26 DDD/100 admissions after the initiation of COVID-19 pandemic. The most utilized antibacterials were piperacillin and enzyme inhibitors in pre-COVID-19 period, whereas meropenem was utilized the most during COVID-19 period. Azithromycin utilization increased by 656.24%, whereas clarithromycin utilization decreased by 52.12%. Antibacterials were utilized most in general surgery department, with an increase of 17.57%. Conclusion: There is an increase in antibacterial utilization in KUH during COVID-19 pandemic, especially reserved antibacterials, which is a concern for antibacterial resistance.
Relating university students’ online self-regulated English learning to motivational beliefs: a structural equation modelling analysis

ABSTRACT This study investigated university students’ online self-regulated English learning (OSEL) and related it to their motivational beliefs in order to probe important psychological factors that may predict how students use self-regulated strategies to facilitate their online English learning. The data was gathered from 337 Thai university nursing students. The instruments used in this study were validated to measure motivational beliefs and OSEL in the context of Thai university students. All motivational belief factors were significantly associated with OSEL. The results of confirmatory factor analysis (CFA) indicated that the construct validity of the questionnaire items was acceptable. Furthermore, the structural modelling analysis (SEM) showed that intrinsic value and cost were significant predictors of all OSEL strategies, namely, behavioural strategies, goal setting, and environment structuring. Meanwhile, academic self-efficacy and attainment value were positively related to certain OSEL factors. Implications for enhancing students’ motivation and self-regulation in online language learning as well as directions for further investigations are discussed.