Miracle-dz/UniFAR-FTUs huggingface dataset:
Nuclear import by an ADAR1 double stranded RNA binding domain

RNA-spezifische Adenosin-Deaminasen (ADARs) beeinflussen die Stabilitat, Struktur und Lokalisation von RNAs durch RNA-Editierung. Sauger-ADAR1 ist ein nukleo-cytoplsmatisch shuttelndes Protein, welches drei doppelstrang-RNA Bindungsdomanen (dsRBDs) beinhaltet. Die dritte dsRBD in ADAR1 ist die erste Domane dieses Typs, welche Kernlokalisation mediiert. In fruheren Studien konnten wir zeigen, dass Kernimport durch Transportin 1 (Trn1) vermittelt wird und durch RNA-Bindung inhibiert wird. Die strukturellen Eigenschaften dieser Domane waren jedoch unbekannt. In dieser Doktorarbeit wurde im Rahmen einer Kollaboration die Struktur dieser Domane mittels NMR ermittelt. Die Struktur zeigt eine dsRBD Faltung mit einer zusatzlichen alpha-Helix am Aminoterminus sowie weitere unstrukturierte Elemente an beiden Enden der Domane. In vitro Mutagenese zeigt, dass die zwei flankierenden Regionen ein bimodulares Kernlokalisationssignal bilden. Die aminoterminale alpha Helix verandert dabei die relative Lage des N- und C-Terminus der dsRBD und bringt beide Enden nahe zueinander. Ich konnte ein neues bimodulares NLS erzeugen, indem ich die ADAR1-dsRBD3 durch eine andere dsRBD oder ein kurzes Peptid ersetzte, wobei die Lage der N- und C-terminalen Segmente unverandert blieb. Dies zeigt, dass die dsRBD nicht fur die Trn1 Interaktion benotigt wird, sondern lediglich die Position der zwei NLS-Module zueinander gewahrleistet. Diese Beobachtung wird durch in-vitro Import - und Koprazipitationsversuche verifiziert. Es werden also die zwei flexiblen Fragmente welche die ADAR1-dsRBD bestimmen an einer Seite der Domane exponiert. Diese werden von Trn1 uber die gleichen Kontaktpunkte gebunden, wie andere Trn1 Substrate. In der Gegenwart von doppelstrang RNA ist die Interaktion mit Trn1 inhibiert. Modelle zeigen, dass die dsRBD in Gegenwart von RNA vermutlich nicht in die Bindungstasche von Trn1 eindringen kann.
THE ROBUSTNESS OF HOMOGENEITY TESTS IN 2 X N TABLES.

SUMMARY A Monte Carlo investigation of 2 X n tables with fixed marginals has been performed. The results of the Monte Carlo distribution show that the probability of Type I error given by the conventional x2 test is in general conservative for 5 or more degrees of freedom even when expectations of successes are very small in each
Teacher feedback literacy, feedback regimes and iterative change: towards enhanced value in feedback processes

ABSTRACT This paper discusses teacher capacities for implementing learning-focused feedback processes within the social contexts of feedback regimes. Data are derived from longitudinal interviews carried out with six recipients of an award for good feedback practice; supplemented by documentary analysis of feedback artefacts; and an interview with the key instigator of the feedback award. Key elements of teacher feedback literacy are highlighted: designing feedback processes for student uptake; reflecting upon students’ responses to feedback; and iterative refinement of practices over time on the basis of cumulative experience. Engaging reflectively with students’ struggles with feedback potentially encourages teachers to adjust practices in the direction of learning-focused feedback processes, indicative of reflective feedback literacy. Broader social contexts in which feedback processes are enacted, including disciplinary and workload-related factors, are influenced by the feedback regimes that hold practices in place. Feedback regimes underpin the social practice of feedback in highlighting routines and procedures facilitating or constraining learning-focused feedback processes. Change elements of salience, congruence and value cast light on prospects for structural refinements to feedback regimes. A value perspective on change envisages making feedback practices satisfying for teachers as well as useful for students.
Electrical Conductivity and Total Emission Coefficient of Air Plasma

The electrical conductivity, o-, and the total radiant power per unit volume, PR9 of air are measured for temperatures between 8,000 and 12,500°K at atmospheric pressure. The measurements are made in a wallstabilized electric arc discharge which produces a pure, stable plasma. Axial electric field strength, £,, and azimuthal magnetic field intensity, B^ are measured as functions of the discharge radius by using probe techniques. Current density, jz9 is obtained as ~]*= V x£//z0, and electrical conductivity is determined by using Ohm's law, o = jJEz. PR is measured with a total radiation thermopile and also by using an electrical technique. Plasma temperature is determined from the absolute intensities of the 4935 A NI and 4368 A OI lines.
Indian summer monsoon prediction and simulation in CFSv2 coupled model

Using carefully designed coupled model experiments, we have demonstrated that the prediction skill of the all India summer monsoon rainfall (AISMR) in Climate Forecast System version 2 (CFSv2) model basically comes from the El‐Niňo Southern Oscillation‐Monsoon teleconnection. On the other hand, contrary to observations, the Indian Ocean coupled dynamics do not have a crucial role in controlling the prediction skill of the AISMR in CFSv2. We show that the inadequate representation of the Indian Ocean coupled dynamics in CFSv2 is responsible for this dichotomy. Hence, the improvement of the Indian Ocean coupled dynamics is essential for further improvement of the AISMR prediction skill in CFSv2.
On a generalized storage model with moment assumptions

This paper considers a semi-infinite storage model, of the type studied by Senturia and Puri [13] and Balagopal [2], defined on a Markov renewal process, {(Xn, Tn ), n = 0, 1, ·· ·}, with 0 = T 0 < T 1 < · ··, almost surely, where Xn takes values in the set {1, 2, ·· ·}. If at Tn, Xn = j, then there is a random ‘input' Vn (j) (a negative input implying a demand) of ‘type' j, having distribution function Fj (·). We assume that {Vn (j)} is an i.i. d. sequence of random variables, taken to be independent of {(Xn, Tn )} and of {Vn (k)}, for k ≠ j, and that Vn (j) has first and second moments. Here the random variables Vn (j) represent instantaneous ‘inputs' (a negative value implying a demand) of type j for our storage model. Under these assumptions, we establish certain limit distributions for the joint process (Z(t), L(t)), where Z(t) (defined in (2)) is the level of storage at time t and L(t) (defined in (3)) is the demand lost due to shortage of supply during [0, t]. Different limit distributions are obtained for the cases when the ‘average stationary input' ρ, as defined in (5), is positive, zero or negative.
A singularly perturbed delay differential equation modeling nosocomial infections

In this article, we consider a model describing hospital acquired infections. The model derived is a system of delay differential equations. The state variable is formed by the patients and the health care workers components. The system is a slow-fast system where the fast equation corresponds to the health care workers equation. The question addressed in this paper is the convergence to the so-called reduced equations which is a single equation for patients. We investigate both finite time convergence and infinite time convergence (uniformly for all positive time) of the original system to the reduced equation.
MM5 precipitation verification over the pacific northwest during the 1997-99 cool seasons

Abstract Precipitation forecasts from the Pennsylvania State University–National Center for Atmospheric Research fifth-generation Mesoscale Model (MM5) are verified for the 1997–98 and 1998–99 cool seasons over the Pacific Northwest. The MM5 precipitation at 36-, 12-, and 4-km horizontal resolution is compared with over 250 NOAA cooperative observer, snow telemetry (SNOTEL), avalanche, and National Weather Service sites in order to evaluate the effects of increasing horizontal resolution and to document spatial variations in model skill. A noticeable improvement in bias, equitable threat, and root-mean-square (rms) error scores occurs as horizontal resolution is increased from 36- to 12-km resolution; however, going from 12- to 4-km resolution improvements in skill are restricted to the heavy precipitation events (>5.08 cm in 24 h). For light to moderate precipitation events, both the 12- and 4-km domains have significant overprediction over the upper windward slopes of the higher terrain. In contrast, fo.
Monte Carlo Methods for Sampling Classical and Quantum Particles

1 Thesis Overview " Monte Carlo " (MC) methods are nondeterministic algorithms for simulating various phenomena in the physical and social sciences. In this study, we evaluated several MC methods for simulating a light particle in a Lennard-Jones (LJ) fluid. We began this research in possession of a basic code that modeled the fluid and light particle in a canonical ensemble. It used Metropolis sampling to equilibrate the fluid and Path Integral Monte Carlo (PIMC) to sample the light particle. This method has been used successfully to calculate positronium lifetimes in spherical and cylindrical pores, but, like all computer simulations, it has its limitations. The canonical ensemble limits us to a fixed number of fluid atoms, making it difficult to simulate a multi-phase system at coexistence, and Metropolis sampling allows us to simulate only one temperature at a time. Our first major modification to this program was to introduce an option to allow simulation of the fluid within a grand canonical ensemble. Using the Widom Test Particle Insertion Method, we were able to show that these grand canonical simulations agreed with the original canonical simulations for average quantities of density and chemical potential. We then used the grand canonical ensemble simulation to find a point on the phase coexistence curve for the fluid. Metropolis sampling is inefficient at phase coexistence because the simulation is slow to overcome the free energy barriers. Therefore, we next implemented a method that modifies Metropolis sampling by using a weighting function to help the system explore the whole energy space. We were able to show that, given the optimal weighting function, this " Multicanonical sampling " allows the system to reach every point in the space with equal probability, sampling evenly even in systems with high free energy barriers. The usefulness of Multicanonical sampling is limited by a fundamental ignorance of this optimal weighting function. To combat this difficulty, we implemented Wang-Landau sampling, which constructs the density of energy states as the simulation proceeds. The results of a Wang-Landau simulation can therefore be used to find ensemble averages at any temperature. We were able to show that one Wang-Landau simulation for the fluid could generate literature results that would require many different Metropolis simulations. We also implemented Wang-Landau for a light particle in a simple harmonic potential and obtained results matching analytical calculations. The analytical calculation we used was specific to the Path Integral approach, …
Hadron multiplicities in p＋p and p＋Pb collisions at the LHC

Experiments at the Large Hadron Collider（LHC）have measured multiplicity distributions in p+p and p+Pb collisions at a new domain of collision energy.Based on considering an energy-dependent broadening of the nucleon’s density distribution,charged hadron multiplicities are studied with the phenomenological saturation model and the evolution equation dependent saturation model.By assuming the saturation scale has a small dependence on the 3-dimensional root mean square（rms）radius at different energy,the theoretical results are in good agreement with the experimental data from CMS and ALICE collaboration.The predictive results in p+p collisions at s1/2=14 TeV of the LHC are also given.
Orientation-dependent measures of chirality

Chirality occupies a central role in fields ranging from biological self assembly to the design of optical meta-materials. The definition of chirality, as given by lord Kelvin in 1893, associates handedness with the lack of mirror symmetry, the inability to superpose an object on its mirror image. However, the quantification of chirality based on this definition has proven to be an elusive task. The difficulty in quantifying chirality is contrasted by the ease with which one determines the handedness of objects with a well defined axis such as screws and helices. We present table-top demonstrations that show that a single object can be simultaneously left handed and right handed when considered from different directions. The orientation dependence of handedness motivates a tensorial quantification of chirality relating directions to rotations, and suggests an extension of Lord Kelvin's definition. We give explicit examples of such tensorial measures of chirality and show how the tensorial nature of chirality can be probed in experiments and exploited as a design principle for chiral meta-materials.
Tumor Lysis, Adverse Events, and Dose Adjustments in 297 Venetoclax-Treated CLL Patients in Routine Clinical Practice

Purpose: Clinical trials of venetoclax reported negligible rates of clinical tumor lysis syndrome (TLS) in patients with chronic lymphocytic leukemia (CLL) when using an extended dose escalation schedule. We aimed to understand TLS prophylaxis, rates of select adverse events (AE), and impact of dosing modifications in routine clinical practice. Experimental Design: This retrospective cohort study included 297 CLL venetoclax-treated patients outside of clinical trials in academic and community centers. Demographics, baseline disease characteristics, venetoclax dosing, TLS risk and prophylaxis, and AEs were collected. Results: The group was 69% male, 96% had relapsed/refractory CLL, 45% had deletion chromosome 17p, 84% had unmutated IGHV, 80% received venetoclax monotherapy, and median age was 67. TLS risk was categorized as low (40%), intermediate (32%), or high (28%), and 62% had imaging prior to venetoclax initiation. Clinical TLS occurred in 2.7% of patients and laboratory TLS occurred in 5.7%. Pre-venetoclax TLS risk group and creatinine clearance independently predict TLS development in multivariable analysis. Grade 3/4 AEs included neutropenia (39.6%), thrombocytopenia (29.2%), infection (25%), neutropenic fever (7.9%), and diarrhea (6.9%). Twenty-two patients (7.4%) discontinued venetoclax due to an AE. Progression-free survival was similar regardless of number of dose interruptions, length of dose interruption, and stable venetoclax dose. Conclusions: These data provide insights into current use of venetoclax in clinical practice, including TLS rates observed in clinical practice. We identified opportunities for improved adherence to TLS risk stratification and prophylaxis, which may improve safety.
Cotargeting of BCL2 with Venetoclax and MCL1 with S63845 Is Synthetically Lethal In Vivo in Relapsed Mantle Cell Lymphoma

Purpose: Mantle cell lymphoma (MCL) is an aggressive subtype of B-cell non-Hodgkin lymphomas characterized by (over)expression of BCL2. A BCL2-targeting drug, venetoclax, has promising anticancer activity in MCL. We analyzed molecular mechanisms of venetoclax resistance in MCL cells and tested strategies to overcome it. Experimental Design: We confirmed key roles of proapoptotic proteins BIM and NOXA in mediating venetoclax-induced cell death in MCL. Both BIM and NOXA are, however, differentially expressed in cell lines compared with primary cells. First, NOXA protein is significantly overexpressed in most MCL cell lines. Second, deletions of BIM gene harbored by three commonly used MCL cell lines (JEKO-1, MINO, and Z138) were not found by array comparative genomic hybridization using a validation set of 24 primary MCL samples. Results: We demonstrated that MCL1 and NOXA play important roles in mediating resistance to venetoclax. Consequently, we tested an experimental treatment strategy based on cotargeting BCL2 with venetoclax and MCL1 with a highly specific small-molecule MCL1 inhibitor S63845. The combination of venetoclax and S63845 demonstrated synthetic lethality in vivo on a panel of five patient-derived xenografts established from patients with relapsed MCL with adverse cytogenetics. Conclusions: Our data strongly support investigation of venetoclax in combination with S63845 as an innovative treatment strategy for chemoresistant MCL patients with adverse cytogenetics in the clinical grounds.
Adropin reduces blood glucose levels in mice by limiting hepatic glucose production

Adropin is a liver‐ and brain‐secreted peptide hormone with striking effects on fuel metabolism regulation in a number of tissues. Previous studies demonstrated that adropin secretion is decreased in obese mice subjected to a long‐term high‐fat diet (HFD), and that whole‐body loss of adropin expression resulted in systemic insulin resistance. Treatment of obese mice with adropin improves glucose tolerance, which has been linked to increased glucose oxidation and inhibition of fatty acid utilization in isolated skeletal muscle homogenates. In this study, we used in vivo physiological measurements to determine how treatment of obese mice with adropin affects whole‐body glucose metabolism. Treatment with adropin reduced fasting blood glucose and, as shown previously, increased glucose tolerance in HFD mice during standard glucose tolerance tests. Under hyperinsulinemic‐euglycemic clamp conditions, adropin treatment led to a nonsignificant increase in whole‐body insulin sensitivity, and a significant reduction in whole‐body glucose uptake. Finally, we show that adropin treatment suppressed hepatic glucose production and improved hepatic insulin sensitivity. This correlated with reduced expression of fatty acid import proteins and gluconeogenic regulatory enzymes in the liver, suggesting that adropin treatment may impact the pathways that drive vital aspects of hepatic glucose metabolism.
Implicit large eddy simulation of a deep cavity using high-resolution methods

Implicit large-eddy simulations of a deep cavity at Mach 0.8 and a Reynolds number based on a cavity length of 860,000 were conducted using a modified high-resolution implicit large-eddy-simulation method. The object of the paper is to assess the capability of the modified numerical method compared to large-eddy simulation with a selective mixed-scale model and experimental measurements of the mean flowfield, Reynolds stresses, and pressure spectra. The frequency and amplitude of the fundamental modes are predicted to within 2% and 6 dB at all grid levels. There is excellent agreement of the mean flowfield and Reynolds stresses, demonstrating that there is no need for an explicit subgrid model when using the new reconstruction method for this flow configuration.
The secret of reducing turnover intention: Evidence from Indonesia

Abstract Turnover still occurs in various organizations, including both profit and non-profit. Accordingly, a study of turnover intention (TI) to detect and anticipate its prevalence among employees is urgently needed. This study explored the impact of psychological capital (PsyCap) and emotional intelligence (EI) on TI mediated by organizational commitment (OC). A survey-operated causal design with a Likert-scale questionnaire was employed to gather data from 439 honorary private school teachers in Indonesia. Structural equation modeling analysis showed that EI and OC negatively affected TI, PsyCap positively affected OC, and PsyCap negatively affected TI through OC. However, PsyCap did not significantly affect TI, EI did not significantly affect OC, and EI did not significantly affect TI mediated by OC, which is inconsistent with previous studies. These inconsistencies create a research gap that requires scientific clarification and confirmation in future research. Practitioners can use this study’s results to reduce TI through the PsyCap, EI, and OC perspectives.
ISSUES IN LES OF WAKE FLOWS

We present a vorticity-based analysis of the turbulent near-wake, which indicates that eddy-viscosity models are inappropriate for sudgrid modeling. In contrast, preliminary a priori tests suggest that scale-similarity models may be a good candidate. We also include a comparison of results from three LES studies available in the literature with our spectral DNS results and with two different sets of experimental results.
Anxiety, insomnia and family support in nurses, two years after the onset of the pandemic crisis

Introduction The Covid-19 pandemic continues to cause serious physical and mental problems for health professionals, particularly nurses. Aim To estimate the prevalence of anxiety and insomnia and to evaluate their possible association with family support received by nurses two years after the onset of the pandemic. Materials and methods In total, the study participants were 404 nurses (335 females and 69 males) with a mean age of 42.88 (SD = 10.9) years and a mean of 17.96 (SD = 12) years working as nurses. Nurses from five tertiary hospitals in Athens constituted the study population who completed the questionnaires State-Trait Anxiety Inventory (STAI), Athens Insomnia Scale (AIS) and Family Support Scale (FSS), in the months of November and December 2021. Regarding demographic and occupational characteristics, gender, age and years of experience as nurses were recorded. Results 60.1% of the nurses showed abnormal scores in state anxiety, with 46.8% in trait anxiety, and 61.4% showed insomnia. Women showed higher scores on the two subscales of anxiety and the insomnia scale compared to men (p < 0.01 and p < 0.05 respectively), while they showed a lower score on the FSS without statistical significance (p > 0.05). Positive correlations (p < 0.01) were found between the State Anxiety Inventory, Trait Anxiety Inventory and AIS, while all of them showed a high negative correlation with FSS (p < 0.01). Age showed a negative correlation with Trait Anxiety Inventory (p < 0.05). As shown by the mediation analysis, the relationship between state anxiety and insomnia was mediated by trait anxiety, whereas state anxiety appeared to be dependent on family support. Conclusions Nurses continue to experience high levels of anxiety and insomnia and feel less supported by their families than in the first year of the pandemic. Insomnia appears to be dependent on state anxiety, with a significant indirect effect of trait anxiety, while family support seems to affect state anxiety.
Commutativity of Two Torsion Free σ-Prime Gamma Rings with Nonzero Derivations

Let M be a 2-torsion free σ-prime Γ-ring and d a nonzero derivation on M. Then M is commutative with the help of the condition [d(x),x]α∈Z(M), for all x∈M and α∈Γ. Let I be a nonzero σ-ideal of M and d a nonzero derivation on M commuting with σ. Then M is commutative in both conditions [d(x),d(y)]α=0 and d(xαy) = d(yαx), for all x,y∈I and α∈Γ.