Miracle-dz/UniFAR-FTUs huggingface dataset:
Deficiency in the production of antibodies to lipids correlates with increased lipid metabolism in severe COVID-19 patients

Background Antibodies to lipids are part of the first line of defense against microorganisms and regulate the pro/anti-inflammatory balance. Viruses modulate cellular lipid metabolism to enhance their replication, and some of these metabolites are proinflammatory. We hypothesized that antibodies to lipids would play a main role of in the defense against SARS-CoV-2 and thus, they would also avoid the hyperinflammation, a main problem in severe condition patients. Methods Serum samples from COVID-19 patients with mild and severe course, and control group were included. IgG and IgM to different glycerophospholipids and sphingolipids were analyzed using a high-sensitive ELISA developed in our laboratory. A lipidomic approach for studying lipid metabolism was performed using ultra-high performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS). Results Mild and severe COVID-19 patients had higher levels of IgM to glycerophosphocholines than control group. Mild COVID-19 patients showed higher levels of IgM to glycerophosphoinositol, glycerophosphoserine and sulfatides than control group and mild cases. 82.5% of mild COVID-19 patients showed IgM to glycerophosphoinositol or glycerophosphocholines plus sulfatides or glycerophosphoserines. Only 35% of severe cases and 27.5% of control group were positive for IgM to these lipids. Lipidomic analysis identify a total of 196 lipids, including 172 glycerophospholipids and 24 sphingomyelins. Increased levels of lipid subclasses belonging to lysoglycerophospholipids, ether and/or vinyl-ether-linked glycerophospholipids, and sphingomyelins were observed in severe COVID-19 patients, when compared with those of mild cases and control group. Conclusion Antibodies to lipids are essential for defense against SARS-CoV-2. Patients with low levels of anti-lipid antibodies have an elevated inflammatory response mediated by lysoglycerophospholipids. These findings provide novel prognostic biomarkers and therapeutic targets.
Effect of estradiol and predator cues on behavior and brain responses of captive female house sparrows (Passer domesticus)

The presence of predators can cause major changes in animal behavior, but how this interacts with hormonal state and brain activity is poorly understood. We gave female house sparrows (Passer domesticus) in post-molt condition an estradiol (n = 17) or empty implant (n = 16) for 1 week. Four weeks after implant removal, a time when female sparrows show large differences in neuronal activity to conspecific vs. heterospecific song, we exposed birds to either 30 min of conspecific song or predator calls, and video recorded their behavior. Females were then euthanized, and we examined neuronal activity using the expression of the immediate early gene (IEG) ZENK to identify how the acoustic stimuli affected neuronal activation. We predicted that if female sparrows with estradiol implants reduce neuronal activity in response to predator calls as they do to neutral tones and non-predatory heterospecifics, they would show less fear behavior and a decreased ZENK response in brain regions involved in auditory (e.g., caudomedial mesopallium) and threat perception functions (e.g., medial ventral arcopallium) compared to controls. Conversely, we predicted that if females maintain auditory and/or brain sensitivity towards predator calls, then female sparrows exposed to estradiol would not show any differences in ZENK response regardless of playback type. We found that female sparrows were less active during predator playbacks independent of hormone treatment and spent more time feeding during conspecific playback if they had previously been exposed to estradiol. We observed no effect of hormone or sound treatment on ZENK response in any region of interest. Our results suggest that female songbirds maintain vigilance towards predators even when in breeding condition.
Biomechanical study of two-level oblique lumbar interbody fusion with different types of lateral instrumentation: a finite element analysis

Objective The aim of this study was to verify the biomechanical properties of a newly designed angulated lateral plate (mini-LP) suited for two-level oblique lumbar interbody fusion (OLIF). The mini-LP is placed through the lateral ante-psoas surgical corridor, which reduces the operative time and complications associated with prolonged anesthesia and placement in the prone position. Methods A three-dimensional nonlinear finite element (FE) model of an intact L1–L5 lumbar spine was constructed and validated. The intact model was modified to generate a two-level OLIF surgery model augmented with three types of lateral fixation (stand-alone, SA; lateral rod screw, LRS; miniature lateral plate, mini-LP); the operative segments were L2–L3 and L3–L4. By applying a 500 N follower load and 7.5 Nm directional moment (flexion-extension, lateral bending, and axial rotation), all models were used to simulate human spine movement. Then, we extracted the range of motion (ROM), peak contact force of the bony endplate (PCFBE), peak equivalent stress of the cage (PESC), peak equivalent stress of fixation (PESF), and stress contour plots. Results When compared with the intact model, the SA model achieved the least reduction in ROM to surgical segments in all motions. The ROM of the mini-LP model was slightly smaller than that of the LRS model. There were no significant differences in surgical segments (L1–L2, L4–L5) between all surgical models and the intact model. The PCFBE and PESC of the LRS and the mini-LP fixation models were lower than those of the SA model. However, the differences in PCFBE or PESC between the LRS- and mini-LP-based models were not significant. The fixation stress of the LRS- and mini-LP-based models was significantly lower than the yield strength under all loading conditions. In addition, the variances in the PESF in the LRS- and mini-LP-based models were not obvious. Conclusion Our biomechanical FE analysis indicated that LRS or mini-LP fixation can both provide adequate biomechanical stability for two-level OLIF through a single incision. The newly designed mini-LP model seemed to be superior in installation convenience, and equally good outcomes were achieved with both LRS and mini-LP for two-level OLIF.
The impact of nutrition on visual cognitive performance in the nutrition, vision, and cognition in sport study

Introduction The purpose of this study was to examine the influence of nutritional intake on visual perceptual-cognitive performance (VCP) in young healthy adults. Methods Ninety-eight healthy men (n = 38) and women (n = 60) aged 18–33 years participated and maintained their usual dietary intake throughout the study. VCP was measured using the NeuroTracker™ CORE (NT) 3-Dimensional (3-D) software program (15 training sessions) over a 15-day period. Food logs and extensive lifestyle measures including body composition, cardiovascular health, sleep and exercise patterns, and general readiness to perform were collected. Mean intake from 10 food logs collected over the 15 days were analyzed using Nutribase software. Statistical analyses were performed in SPSS using repeated measures ANOVA including significant covariates when appropriate. Results Males consumed significantly more calories, macronutrients, cholesterol, choline, and zinc and performed significantly better on VCP than the females. Participants who consumed more than 40% of kcals from carbohydrates (p = 0.038), less than 24% of kcals from protein (p = 0.009), more than 2,000 μg/day lutein/zeaxanthin or more than 1.8 mg/ day vitamin B2 performed significantly better on VCP than those who consumed less than those amounts, respectively. Discussion VCP is an important dimension of cognitive function and in the present study is influenced by higher carbohydrate, lutein/ zeaxanthin, and vitamin B2 dietary intake while high protein consumption and the female sex negatively impacted VCP.
Association between bone mineral density and cardiovascular disease in older adults

Background and aims Cardiovascular disease and osteoporosis are common diseases in older adults with high morbidity. The study on the interaction between the two in pathogenic mechanisms has been paid much attention by the majority of researchers. This study aimed to explore the relationship between bone mineral density and cardiovascular disease in older adults. Methods The primary data was downloaded from the National Health and Nutrition Examination Survey database of the United States. Multivariate logistic regression model, generalized additive model, and smooth curve fitting were used to explore the relationship between bone mineral density and cardiovascular events risk. When a curve relationship was found, a two-piecewise linear model was used to calculate the inflection point. In addition, subgroup analysis was also performed. Results A total of 2097 subjects were included in this study. After adjusting for potential confounders, no significant association was found between lumbar bone mineral density and cardiovascular disease, while femur bone mineral density had a non-linear relationship with cardiovascular disease, with an inflection point of 0.741 gm/cm2. When bone mineral density was <0.741 gm/cm2, the risk of cardiovascular disease decreased speedily. Once bone mineral density exceeded this value, the risk of cardiovascular disease continued to decrease, but the trend became significantly slower. Compared with patients with normal bone mass, osteoporosis was associated with a 2.05-fold increased risk of cardiovascular disease (95% CI 1.68–5.52). There were no significant differences in interaction tests of all subgroups (p for interaction >0.05) except race. Conclusion Our results indicated that bone mineral density was closely associated with the prevalence of cardiovascular disease in older adults over 60 years old, especially the femur bone mineral density was negatively non-linear associated with cardiovascular disease risk, with an inflection point of 0.741 gm/cm2.
Molecular epidemiological characteristics of Brucella in Guizhou Province, China, from 2009 to 2021

Introduction Brucellosis was made statutorily notifiable in 1955, in China, while in Guizhou Province, the pathogen of human brucellosis was isolated for the first time in 2011. However, currently, the brucellosis epidemic is becoming more and more severe in Guizhou Province. The type distribution and genetic characteristics of Brucella in Guizhou Province, as well as its evolutionary relationship with domestic and foreign strains, are still unclear. Methods MLST, MLVA, and rpoB typing techniques were used for the molecular epidemiological study of the 83 Brucella isolates in Guizhou province. Results Among the 83 Brucella strains, MLST identified three ST genotypes, of which ST39 is a newly reported type in China. MLVA-16 generated 49 genotypes, and MLVA-11 generated 5 known genotypes and 2 unreported genotypes. Six genotypes were identified by rpoB technology. Discussion MLVA has a high resolution, but differences at the Bruce 04 and 16 loci cannot exclude associations between epidemics, and combining MLST and rpoB typing methods for epidemiologic tracing can avoid erroneous judgments. Moreover, through the combined analysis of the three typing techniques, the possible origin of the new Brucella can be reasonably inferred, which is also conducive to promoting the subsequent research of the novel Brucella.
Novel evidence on sepsis-inducing pathogens: from laboratory to bedside

Sepsis is a life-threatening condition and a significant cause of preventable morbidity and mortality globally. Among the leading causative agents of sepsis are bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, and Streptococcus pyogenes, along with fungal pathogens of the Candida species. Here, we focus on evidence from human studies but also include in vitro and in vivo cellular and molecular evidence, exploring how bacterial and fungal pathogens are associated with bloodstream infection and sepsis. This review presents a narrative update on pathogen epidemiology, virulence factors, host factors of susceptibility, mechanisms of immunomodulation, current therapies, antibiotic resistance, and opportunities for diagnosis, prognosis, and therapeutics, through the perspective of bloodstream infection and sepsis. A list of curated novel host and pathogen factors, diagnostic and prognostic markers, and potential therapeutical targets to tackle sepsis from the research laboratory is presented. Further, we discuss the complex nature of sepsis depending on the sepsis-inducing pathogen and host susceptibility, the more common strains associated with severe pathology and how these aspects may impact in the management of the clinical presentation of sepsis.
Rumen microbiome response to sustained release mineral bolus supplement with low- and high-quality forages

Introduction Limited forage quantity and quality are challenges faced in livestock production systems in semi-arid rangelands of the western United States, particularly when livestock face stressors such as cold weather or have increased nutritional requirements such as during pregnancy and lactation. To meet livestock nutrient requirements, producers frequently provide supplemental nutrition, however there is limited knowledge regarding the effects of these practices on the rumen microbiome in these environments. Methods A study was conducted to evaluate changes in the rumen microbiome in response to high- and low- quality forage with sustained release mineral boluses. The study consisted of 16 ruminally-cannulated 2–3-year-old black angus cows fed high quality grass alfalfa hay or low-quality grass hay with a 90 or 180 day sustained release mineral bolus. Rumen samples were collected pre-feeding and 8 hours post feeding and bacterial 16S rRNA gene amplicons were sequenced from the rumen fluid. Results Alpha diversity as measured by Shannon’s diversity index decreased significantly over time (p<0.01) and averaged 5.6 pre-feeding and 5.4 post- feeding and was not significantly different between high- and low-quality forages or between mineral bolus types (p>0.05). Principal coordinates analysis (PCoA) of the Bray-Curtis dissimilarity matrix showed distinct grouping by feed quality and time but not by mineral bolus type. Bacteroidetes and Firmicutes were the dominant phyla in all treatments and significant increases (p<0.05) in the relative abundance of the family Lachnospiraceae and the genus Prevotella were observed in high quality forage diets. Rumen VFA and NH3-N concentrations were also strongly associated with the high-quality forage diet. Predictive functional profiling indicated that functions associated with methanogenesis were negatively correlated with feed quality. Discussion The results of this study suggest that mineral bolus type is unlikely to affect rumen bacterial community structure or function while forage quality can significantly alter community structure and predicted functions associated with methanogenesis and VFA production.
Key driver of textile and apparel industry management: fashion brand ESG and brand reputation

The aim of this study was to develop and validate multidimensional criteria that can be used to evaluate fashion brand ESG management. This research used both qualitative and quantitative research methods to derive multi-dimensional and wide-ranging questions that could help explain fashion brand ESG with a high level of detail. A Delphi study was conducted with a group of 30 professionals to derive the initial items for fashion brand ESG management, and these items were used to design a questionnaire that was then administered to 800 consumers. Based on the results of exploratory and confirmatory factor analysis, 13 items were used to construct the scale. Convergent and discriminant validity were also verified between the factors. Finally, it was confirmed that the items on the ESG practices scale significantly affected a fashion brand’s reputation and consumer intention to purchase that brand as mediated by reputation. The results of this research are expected to provide a theoretical framework for future ESG research that can help fashion brands achieve more effective ESG management and increase their reputation and sales.
Association of parent-child interactions with parental psychological distress and resilience during the COVID-19 pandemic

Introduction The effects of psychological distress/resilience on parent-child engagement (e.g., family dinners, reading) during the COVID-19 pandemic have not been well studied. Among very young children from underrepresented backgrounds enrolled in the ongoing longitudinal Bronx Mother Baby Health Study of healthy term infants, we (1) examined associations between exposures to COVID-19-related events, demographic factors and parental psychological distress and resilience; and (2) correlated these factors with parent-child engagement activities. Methods Between June 2020-August 2021, parents of 105 Bronx Mother Baby Health Study participants aged birth-25 months completed questionnaires related to exposures to COVID-19-related events, frequency of positive parent-child engagement activities, food and housing insecurity, and parental psychological distress and resilience. Families were also asked open ended questions about the pandemic's impact. Results 29.8% and 47.6% of parents reported food and housing insecurity, respectively. Greater exposures to COVID-19-related events were associated with increased parental psychological distress. Positive parent-child interactions were associated with demographic factors and higher levels of maternal education, but not with exposures to COVID-19-related events. Discussion This study adds to a growing body of literature on the negative impacts of COVID-19 exposures and psychosocial stressors on families during the pandemic, supporting the need for enhanced mental health resources and social supports for families.
Mesoscale Convective Systems in Weakly Forced Large-Scale Environments. Part I: Observations

Abstract During a 24-h period, beginning 1200 UTC 11 May 1982, a series of five mesoscale convective systems (MCSs) developed within a weakly forced large-scale environment. Analyses indicate that the large-scale flow created a broad region of potential buoyant energy, but owing to a restraining inversion and weak large-scale upward motion, convection was initiated only where lifting associated with mesoscale features was able to eliminate the inversion. The series of MCSs developed sequentially and moved eastward across the moist axis. Two of these systems had a large component of motion against the mean tropospheric flow and propagated in a direction nearly opposite to that of the traveling upper-level disturbances. Each system produced an outflow of cold downdraft air that spread progressively farther south than that from the preceding system. This description of the development and evolution of convection is very different from traditional ones wherein convection develops and moves more or less in pha.
Statistical Models for the Evaluation of Portable Lead Measurement Technologies-Part I: Chemical Test Kits

regression model in order to describe test kit performance. This article discusses estimation of the OC curves from numerical and stochastic points of view. The use of nonparametric estimates in graphical and quantitative analyses is also discussed. Attention is given to the fact that the true lead levels were themselves estimated by a process that was affected by paint collection and laboratory analysis procedures.
Entanglement transition and replica wormholes in the dissipative Sachdev-Ye-Kitaev model

Recent discoveries have highlighted the significance of replica wormholes in resolving the information paradox and establishing the unitarity of black hole evaporation. In this letter, we propose the dissipative Sachdev-Ye-Kitaev model (SYK) as a minimal quantum model that exhibits entanglement dynamics with features qualitatively similar to replica wormholes. As a demonstration, we investigate the entanglement growth of a pair of dissipative SYK models initialized in a thermofield double state (TFD). In the regime of large $N$ with weak dissipation, we observe a first-order entanglement transition characterized by a switch of the dominant saddle point: from replica diagonal solutions for short times to replica wormhole-like off-diagonal solutions for long times. Furthermore, we show that signature of replica wormholes persists even at moderate $N \lesssim 30$ by using the Monte Carlo quantum trajectory method. Our work paves the way for explorations of replica wormhole physics in quantum simulators.
Maximizing wind farm power output with the helix approach: Experimental validation and wake analysis using tomographic particle image velocimetry

Wind farm control can play a key role in reducing the negative impact of wakes on wind turbine power production. The helix approach is a recent innovation in the field of wind farm control, which employs individual blade pitch control to induce a helical velocity profile in a wind turbine wake. This forced meandering of the wake has turned out to be very effective for the recovery of the wake, increasing the power output of downstream turbines by a significant amount. This paper presents a wind tunnel study with two scaled wind turbine models of which the upstream turbine is operated with the helix approach. We used tomographic particle image velocimetry to study the dynamic behavior of the wake under the influence of the helix excitation. The measured flow fields confirm the wake recovery capabilities of the helix approach compared with normal operation. Additional emphasis is put on the effect of the helix approach on the breakdown of blade tip vortices, a process that plays an important role in re‐energizing the wake. Measurements indicate that the breakdown of tip vortices and the resulting destabilization of the wake are enhanced significantly with the helix approach. Finally, turbine measurements show that the helix approach was able to increase the combined power for this particular two‐turbine setup by as much as 15%.
Constraints on the spectrum of HESS J0632+057 from Fermi-LAT data

We report on the results of ~7 yrs of the very-high energy (10-600 GeV) observations of HESS J0632+057 with FERMI/LAT. In the highest energy band, 200-600 GeV, the source is clearly detected with the statistical significance >3.6sigma at orbital phases 0.2-0.4 and 0.6-0.8 at which HESS J0632+057 is known to demonstrate enhanced emission in TeV energy band. The analysis did not reveal the emission from HESS J0632+057 at lower energies and different orbital phases. Using the upper limits on source's flux we locate the break of the spectrum to >80 GeV and low-energy slope <2.0 (2sigma statistical significance).