Miracle-dz/UniFAR-FTUs huggingface dataset:
Study on anti-decay ability of modified fast-grown poplar with phenol-formaldehyde resin

The natural anti-decay ability of untreated poplar samples was quite poor,while the performance of those samples modified with low molecule phenol-formaldehyde resin(PF) could be enhanced distinctly.8 weeks later,weight percent losses(WPL) of untreated samples reached 9.52%,WPL of treated samples was all less than 2%,and could be the lowest when the weight percent gains were adopted at the level of 14.2%. The color change of modified and un-modified samples validated this point further.The conclusions could be drawn that the anti-decay ability of fast-grown poplar modified with PF could be improved clearly.
Angiotensin-converting enzyme 2: a key enzyme in key organs

2020 marked the 20th anniversary of the discovery of the angiotensin-converting enzyme 2 (ACE2). This major event that changed the way we see the renin–angiotensin system today could have passed quietly. Instead, the discovery that ACE2 is a major player in the severe acute respiratory syndrome coronavirus 2 pandemic has blown up the literature regarding this enzyme. ACE2 connects the classical arm renin–angiotensin system, consisting mainly of angiotensin II peptide and its AT1 receptor, with a protective arm, consisting mainly of the angiotensin 1–7 peptide and its Mas receptor. In this brief article, we have reviewed the literature to describe how ACE2 is a key protective arm enzyme in the function of many organs, particularly in the context of brain and cardiovascular function, as well as in renal, pulmonary and digestive homeostasis. We also very briefly review and refer to recent literature to present an insight into the role of ACE2 in determining the course of coronavirus diseases 2019.
Therapists’ Experience of Video Consultation in Specialized Mental Health Services During the COVID-19 Pandemic: Qualitative Interview Study

Background As part of political and professional development with increased focus on including service users within mental health services, these services are being transformed. Specifically, they are shifting from institutional to noninstitutional care provision with increased integration of the use of electronic health and digitalization. In the period from March to May 2020, COVID-19 restrictions forced rapid changes in the organization and provision of mental health services through the increased use of digital solutions in therapy. Objective The aim of this study was to develop and advance comprehensive knowledge about how therapists experience the use of video consultation (VC). To reach this objective, we evaluated therapists’ experiences of using VC in specialized mental health services in the early phase of COVID-19 restrictions. The following questions were explored through interviews: Which opportunities and challenges appeared when using VC during the period of COVID-19 restrictions? In a short-term care pathway, for whom does VC work and for whom does it not work? Methods This study employed a qualitative approach based on an abductive strategy and hermeneutic-phenomenological methodology. Therapists and managers in mental health departments in a hospital were interviewed via Skype for Business from March to May 2020, using a thematic interview guide that aimed to encourage reflections on the use of VC during COVID-19 restrictions. Results Therapists included in this study experienced advantages in using VC under circumstances that did not permit face-to-face consultations. The continuity that VC offered the service users was seen as a valuable asset. Various negative aspects concerning the therapeutic environment such as lack of safety for the most vulnerable service users and topics deemed unsuitable for VC lowered the therapists’ overall impression of the service. The themes that arose in the data analysis have been categorized in the following main topics: (1) VC—“it’s better than nothing”; (2) VC affects therapists’ work situation—opportunities and challenges in working conditions; and (3) challenges of VC when performing professional assessment and therapy on the screen. Conclusions Experiences with VC in a mental health hospital during COVID-19 restrictions indicate that there are overall advantages to using VC when circumstances do not permit face-to-face consultations. Nevertheless, various negative aspects in the use of VC lowered the therapists’ overall impression of VC. Further qualitative research is needed, and future studies should focus on service users’ experiences, cocreation between different stakeholders, and how to scale up the use of VC while ensuring that the service provided is appropriate, safe, and available.
Effect of samul-tang on female fertility via RAS signaling pathway in ovaries of aged mice

Samul-tang (SM), a traditional herbal medicine, is used to treat age-related human conditions, such as infertility and menstrual irregularities. The mechanism underlying the role of SM in ovary function needs elucidation. In this study, the influence of SM administration on the ovarian reserve of aged mice was investigated. Female BALB/c mice (8 and 40 weeks-old) were administered with distilled water (young or old group) or SM for 4 weeks. SM administration prevented age-related ovarian follicle loss in mice. Quality of oocytes and blastocysts were enhanced in SM-administrated mice compared to those of non-treated old mice. Further, SM administration increased the pregnancy rate and number of litters. SM triggered changes in aging-related genes that are linked to the RAS-mediated pathway. Thus, we demonstrate that SM can be used to increase the oocyte yield in aged women, potentially improving age-related cognitive decline in the ovarian reserve.
M. tuberculosis PknG manipulates host autophagy flux to promote pathogen intracellular survival

ABSTRACT The eukaryotic-type protein kinase G (PknG), one of the eleven eukaryotic type serine-threonine protein kinase (STPK) in Mycobacterium tuberculosis (Mtb), is involved in mycobacterial survival within macrophages, presumably by suppressing phagosome and autophagosome maturation, which makes PknG an attractive drug target. However, the exact mechanism by which PknG inhibits pathogen clearance during mycobacterial infection remains largely unknown. Here, we show that PknG promotes macroautophagy/autophagy induction but inhibits autophagosome maturation, causing an overall effect of blocked autophagy flux and enhanced pathogen intracellular survival. PknG prevents the activation of AKT (AKT serine/threonine kinase) via competitively binding to its pleckstrin homology (PH) domain, leading to autophagy induction. Remarkably, PknG could also inhibit autophagosome maturation to block autophagy flux via targeting host small GTPase RAB14. Specifically, PknG directly interacts with RAB14 to block RAB14-GTP hydrolysis. Furthermore, PknG phosphorylates TBC1D4/AS160 (TBC1 domain family member 4) to suppress its GTPase-activating protein (GAP) activity toward RAB14. In macrophages and in vivo, PknG promotes Mtb intracellular survival through blocking autophagy flux, which is dependent on RAB14. Taken together, our data unveil a dual-functional bacterial effector that tightly regulates host autophagy flux to benefit pathogen intracellular survival. Abbreviations: AKT: AKT serine/threonine kinase; ATG5: autophagy related 5; BMDMs: bone marrow-derived macrophages; DTT: dithiothreitol; FBS: fetal calf serum; GAP: GTPase-activating protein; MOI: multiplicity of infection; Mtb: Mycobacterium tuberculosis; MTOR: mechanistic target of rapamycin kinase; OADC: oleic acid-albumin-dextrose-catalase; PC, phosphatidylcholine; PH: pleckstrin homology; PI3K: phosphoinositide 3-kinase; PknG: protein kinase G; PtdIns(3,4,5)P3: phosphatidylinositol(3,4,5)-trisphosphate; SQSTM1: sequestosome 1; STPK: serine-threonine protein kinase; TB: tuberculosis; TBC1D4: TBC1 domain family member 4; TPR: tetratricopeptide repeat; ULK1: unc-51 like autophagy activating kinase 1; WT: wild-type
Identification of an RNA sponge that controls the RoxS riboregulator of central metabolism in Bacillus subtilis

Abstract sRNAs are a taxonomically-restricted but transcriptomically-abundant class of post-transcriptional regulators. While of major importance for adaption to the environment, we currently lack global-scale methodology enabling target identification, especially in species without known RNA hub proteins (e.g. Hfq). Using psoralen RNA cross-linking and Illumina-sequencing we identify RNA–RNA interacting pairs in vivo in Bacillus subtilis, resolving previously well-described interactants. Although sRNA–sRNA pairings are rare (compared with sRNA–mRNA), we identify a robust example involving the conserved sRNA RoxS and an unstudied sRNA RosA (Regulator of sRNA A). We show RosA to be the first confirmed RNA sponge described in a Gram-positive bacterium. RosA interacts with at least two sRNAs, RoxS and FsrA. The RosA/RoxS interaction not only affects the levels of RoxS but also its processing and regulatory activity. We also found that the transcription of RosA is repressed by CcpA, the key regulator of carbon-metabolism in B. subtilis. Since RoxS is already known to be transcriptionally controlled by malate via the transcriptional repressor Rex, its post-transcriptional regulation by CcpA via RosA places RoxS in a key position to control central metabolism in response to varying carbon sources.
Modelling the structural variation of quartz and germanium dioxide with temperature by means of transformed crystallographic data

A pseudocubic parameterization of the O4 tetrahedra of quartz and its structural analogue GeO2 at variable temperatures allows direct calculation of tetrahedral tilt angle, the microscopic order parameter of the α↔β phase transition. The crystal structures at interpolated or extrapolated temperatures can also be predicted.
Femtosecond laser–assisted descemetorhexis for Descemet membrane endothelial keratoplasty: cell-based and tissue-based ex vivo analysis of precision and safety

Femtosecond laser–assisted descemetorhexis could serve as a safe and precise technique in DMEK surgery to facilitate standardized, reproducible results with only minimal endothelial cell damage. Purpose: To analyze precision and safety of femtosecond laser–assisted descemetorhexis and postoperative corneal wound healing in human ex vivo specimens. Setting: Department of Ophthalmology, University Hospital, LMU Munich, Munich, Germany. Design: Experimental ex vivo study. Methods: 4 donor corneas underwent femtosecond laser–assisted descemetorhexis. The descemetorhexis was performed using the LDV Z8 femtosecond laser, followed by live/dead staining, phase contrast microscopy, and scanning electron microscopy. The descemetorhexis parameters were set using a modified optical coherence tomography image capturing of 8 segments within a focus of 100 μm at the posterior corneal stroma (reversed capsulotomy program). Results: Live/dead sample staining analysis demonstrated that the femtosecond laser had minimal impact on the vitality of surrounding endothelial cells. Phase contrast microscopy and scanning electron microscopy evaluation showed that the laser produced precise, clear-cut edges, leaving no stromal tissue bridges. Conclusions: Femtosecond laser–assisted descemetorhexis could serve as a safe and precise technique with only minimal endothelial cell damage. To minimize stromal damage, further laser energy profile adjustments are necessary to optimize corneal treatment within different stages of corneal tissue swelling.
Trajectories of verbal fluency and executive functions in multilingual and monolingual children and adults: A cross-sectional study

The development of verbal fluency is associated with the maturation of executive function skills, such as the ability to inhibit irrelevant information, shift between tasks, and hold information in working memory. Some evidence suggests that multilinguistic upbringing may underpin disadvantages in verbal fluency and lexical retrieval, but can also afford executive function advantages beyond the language system including possible beneficial effects in older age. This study examined the relationship between verbal fluency and executive function in 324 individuals across the lifespan by assessing the developmental trajectories of English monolingual and multilingual children aged 7–15 years (N = 154) and adults from 18 to 80 years old (N = 170). The childhood data indicated patterns of improvement in verbal fluency and executive function skills as a function of age. Multilingual and monolingual children had comparable developmental trajectories in all linguistic and non-linguistic measures used in the study with the exception of planning, for which monolingual children showed a steeper improvement over the studied age range relative to multilingual children. For adults, monolinguals and multilingual participants had comparable performance on all measures with the exception of nonverbal inhibitory control and response times on the Tower of London task: monolinguals showed a steeper decline associated with age. Exploratory factor analysis indicated that verbal fluency was associated with working memory and fluid intelligence in monolingual participants but not in multilinguals. These findings raise the possibility that early acquisition of an additional language may impact on the development of the functional architecture serving high-level human cognition.
The use of virtual reality in assessment and treatment of anxiety and related disorders

Abstract In recent years the development of new virtual environments has been qualitatively high and fast at the same time, but the dissemination of virtual reality (VR) in clinical practice is still scarce. The aim of this review is to give an insight into the state of the art of the use of VR as an assessment tool and treatment intervention in anxiety and related disorders as posttraumatic stress disorder and obsessive–compulsive disorders. Besides an overview into the efficacy of VR, a summary will be given on assumed working mechanisms in virtual reality exposure therapy and how this aligns with current theoretical models. Further, it will be discussed how VR is accepted by patients and research into the reluctance of therapist to use this technology during treatment with focus on the therapeutic alliance and how it may be influenced by the use of VR. Finally, we discuss clinical and future issues as, for example, dissemination into clinical practice and what VR has to offer therapists in future. This not only in adult population but as well in younger patients, as young adolescents VR has a great potential as it connects easily with its playful elements to this population and might be a low threshold step to offer treatment or preventive interventions.
Safety and Gadolinium Distribution of the New High-Relaxivity Gadolinium Chelate Gadopiclenol in a Rat Model of Severe Renal Failure

Supplemental digital content is available in the text. Objective The aim of this study was to investigate the toxicological profile of gadopiclenol, a new high-relaxivity macrocyclic gadolinium-based contrast agent (GBCA), in renally impaired rats, in comparison with 2 other macrocyclic GBCAs, gadoterate meglumine and gadobutrol, and 1 linear and nonionic GBCA, gadodiamide. Methods Renal failure was induced by adding 0.75% wt/wt adenine to the diet for 3 weeks. During the second week of adenine-enriched diet, the animals (n = 8/group × 5 groups) received 5 consecutive intravenous injections of GBCA at 2.5 mmol/kg per injection, resulting in a cumulative dose of 12.5 mmol/kg or saline followed by a 3-week treatment-free period after the last injection. The total (elemental) gadolinium (Gd) concentration in different tissues (brain, cerebellum, femoral epiphysis, liver, skin, heart, kidney, spleen, plasma, urine, and feces) was measured by inductively coupled plasma mass spectrometry. Transmission electron microscopy (and electron energy loss spectroscopy analysis of metallic deposits) was used to investigate the presence and localization of Gd deposits in the skin. Relaxometry was used to evaluate the presence of dissociated Gd in the skin, liver, and bone. Skin histopathology was performed to investigate the presence of nephrogenic systemic fibrosis–like lesions. Results Gadodiamide administrations were associated with high morbidity-mortality but also with macroscopic and microscopic skin lesions in renally impaired rats. No such effects were observed with gadopiclenol, gadoterate, or gadobutrol. Overall, elemental Gd concentrations were significantly higher in gadodiamide-treated rats than in rats treated with the other GBCAs for all tissues except the liver (where no significant difference was found with gadopiclenol) and the kidney and the heart (where statistically similar Gd concentrations were observed for all GBCAs). No plasma biochemical abnormalities were observed with gadopiclenol or the control GBCAs. Histopathology revealed a normal skin structure in the rats treated with gadopiclenol, gadoterate, and gadobutrol, contrary to those treated with gadodiamide. No evidence of Gd deposits on collagen fibers and inclusions in fibroblasts was found with gadopiclenol and its macrocyclic controls, unlike with gadodiamide. Animals of all test groups had Gd-positive lysosomal inclusions in the dermal macrophages. However, the textures differed for the different products (speckled texture for gadodiamide and rough-textured appearance for the 2 tested macrocyclic GBCAs). Conclusions No evidence of biochemical toxicity or pathological abnormalities of the skin was observed, and similar to other macrocyclic GBCAs, gadoterate and gadobutrol, tissue retention of Gd was found to be low (except in the liver) in renally impaired rats treated with the new high-relaxivity GBCA gadopiclenol.
Elevated low-frequency free-stream vortical disturbances eliminate boundary-layer separation

Abstract A steady two-dimensional boundary layer subject to an adverse streamwise pressure gradient usually separates. In this paper, we investigate how free-stream vortical disturbances (FSVD) of moderate level prevent the separation in such a boundary layer over a plate or concave wall. The focus is on physically realisable FSVD with sufficiently long wavelength (low frequency) as they have the most significant impact on the boundary layer. The FSVD intensity $\epsilon$ is taken to be small but nevertheless strong enough that the streaks or Görtler vortices generated in the boundary layer are fully nonlinear and can alter the mean-flow profile by an order-one amount. The excitation and evolution of streaks and Görtler vortices are governed by the nonlinear unsteady boundary-region equations supplemented by appropriate initial (upstream) and boundary (far-field) conditions, which describe appropriately the action of FSVD on the boundary layer. The flow variables are decomposed into two parts: the steady spanwise-averaged and the unsteady or spanwise-varying components. These two parts are coupled and are computed simultaneously. Numerical results show that the separation is eliminated when the FSVD level exceeds a critical intensity $\epsilon _c$. It is inferred that the strong nonlinear mean-flow distortion associated with the nonlinear streaks or Görtler vortices prevents the separation. The critical FSVD intensity $\epsilon _c$ depends on the streamwise curvature, the pressure gradient and the frequency of FSVD. The value of $\epsilon _c$ decreases significantly with the Görtler number, indicating that concave curvature inhibits separation. A higher $\epsilon _c$ is required to prevent the separation in the case of stronger adverse pressure gradient. Interestingly, unsteady FSVD with low frequencies are found to be more effective than steady ones in suppressing the separation.
A taxonomy of antonymy in Arabic: Egyptian and Saudi proverbs in comparison

Abstract This study has set out to identify, quantify, typify, and exemplify the discourse functions of canonical antonymy in Arabic paremiography by comparing two manually collected datasets from Egyptian and Saudi (Najdi) dialects. Building upon Jones’s (2002) most extensive and often-cited classification of the discourse functions of antonyms as they co-occur within syntactic frames in news discourse, the study has substantially revised this classification and developed a provisional and dynamic typology thereof. Two major textual functions are found to be quantitatively significant and qualitatively preponderant: ancillarity (wherein an A-pair of canonical antonyms project their antonymicity onto a more important B-pair) and coordination (wherein one antonym holds an inclusive or exhaustive relation to another antonym). Three new functions have been developed and added to the retrieved classification: subordination (wherein one antonym occurs in a subordinate clause while the other occurs in a main clause), case-marking (wherein two opposite cases are served by two antonyms), and replacement (wherein one antonym is substituted with another). Semicanonical and noncanonical guises of antonymy are left and recommended for future research.
Surface Ultrastructure of the Adult and Juvenile Stages of the Trematode Astiotrema Impletum (Looss, 1899) Looss 1900 (incertae Sedis) from the Nile puffer, Tetraodon Lineatus Linnaeus, 1758

Summary A scanning electron microscope study was performed on the surface of the trematode Astiotrema impletum (Looss, 1899) Looss 1900 from the Nile puffer, Tetraodon lineatus Linnaeus, 1758 (Syn. Tetraodon fahaka) for the first time. Adult A. impletum have a markedly large, sub-terminal oral sucker and a small ventral sucker. As with most trematodes, tegumental spines are concentrated anteriorly and are absent just anterior the ventral sucker. Spines have serrated tips on a short, tongue–shaped body. At the level of the ventral sucker the base of each spine exhibits a three bulbous-like structure that can be partially or completely withdrawn into the tegument. Sensory papillae are concentrated around the oral and ventral suckers and genital pores. Papillae are conical or knob-like, either ciliated or non-ciliated; some are protruded and others are embedded inside the tegument. In the juvenile stage, spines are smaller, less well-developed and tightly packed. The cytoplasmic processes of the posterior quarter of body show brain-like velvety appearance on the adult and small cobblestone-like on the juvenile.