Miracle-dz/UniFAR-FTUs huggingface dataset:
Whole-Genome Analysis of Stress Resistance-Related Genes in Listeria monocytogenes

Listeria monocytogenes is a crucial foodborne pathogen with significant public health implications. This study analyzed whole-genome sequences (WGS) of L. monocytogenes strains from public databases, examining associations between resistance genes, lineage, strain type, isolation source, and geography. Results revealed that after eliminating duplicates and strains with incomplete WGS, a total of 316 strains were deemed suitable for subsequent analyses. Within these strains, lineages I and II were extensively distributed, predominantly isolated from clinical and food sources. 56.65% of these strains fell into seven major Clonal Complexes (CC), identified by Multilocus Sequence Typing (MLST), correlating significantly with isolation information. Analysis of 46 resistance-related genes showed a high consistency of resistance genes in the same type of strains, hinting at a potential causal chain of ‘habits-foods-environments evolutions’. Moreover, the standard strains exhibit similar gene carriage rates as the sample strains, with multiple variations observed in acid-resistance genes. In conclusion, through a comprehensive analysis of the L. monocytogenes genome sequences, this study deepens our understanding of the differences and associations between its lineage, strain typing, isolation sources, geographical distribution, and resistance genes. It has also explored the potential impact of environmental noise on the expression of these genes, offering a scientific foundation for devising more effective prevention and control strategies against L. monocytogenes. Future endeavors should further dissect the functions of stress resistance genes and the variations in their expression, with the aim of gaining a deeper insight into the risks posed by L. monocytogenes to public health safety.
Mapping patterns of thought onto brain activity during movie-watching

Movie watching is a central aspect of our lives and an important paradigm for understanding the brain mechanisms behind cognition as it occurs in daily life. Contemporary views of ongoing thought argue that the ability to make sense of events in the ‘here and now’ depend on the neural processing of incoming sensory information by auditory and visual cortex, which are kept in check by systems in association cortex. However, we currently lack an understanding of how patterns of ongoing thoughts map onto the different brain systems when we watch a film, partly because methods of sampling experience disrupt the dynamics of brain activity and the movie-watching experience. Our study established a novel method for mapping thought patterns onto the brain activity that occurs at different moments of a film, which does not disrupt the time course of brain activity or the movie-watching experience. We found moments when experience sampling highlighted engagement with multi-sensory features of the film or highlighted thoughts with episodic features, regions of sensory cortex were more active and subsequent memory for events in the movie was better—on the other hand, periods of intrusive distraction emerged when activity in regions of association cortex within the frontoparietal system was reduced. These results highlight the critical role sensory systems play in the multi-modal experience of movie-watching and provide evidence for the role of association cortex in reducing distraction when we watch films. Significance statement States like movie-watching provide a window into the brain mechanisms behind cognition in daily life. However, we know relatively little about the mapping between brain activity during movies and associated thought patterns because of difficulties in measuring cognition without disrupting how brain activity naturally unfolds. We establish a novel method to link different experiential states to brain activity during movie-watching with minimal interruptions to viewers or disruptions to brain dynamics. We found states of sensory engagement occur in moments of films when activity in visual and auditory cortex are high. In contrast, states of distraction are reduced when activity in frontoparietal regions is high. Our study, therefore, establishes both sensory and association cortex as core features of the movie-watching experience.
Mitochondrial DNA copy number reduction via in vitro TFAM knockout remodels the nuclear epigenome and transcriptome

Mitochondrial DNA copy number (mtDNA-CN) is associated with several age-related chronic diseases and is a predictor of all-cause mortality. Here, we examine site-specific differential nuclear DNA (nDNA) methylation and differential gene expression resulting from in vitro reduction of mtDNA-CN to uncover shared genes and biological pathways mediating the effect of mtDNA-CN on disease. Epigenome and transcriptome profiles were generated for three independent human embryonic kidney (HEK293T) cell lines harbouring a mitochondrial transcription factor A (TFAM) heterozygous knockout generated via CRISPR-Cas9, and matched control lines. We identified 4,242 differentially methylated sites, 228 differentially methylated regions, and 179 differentially expressed genes associated with mtDNA-CN. Integrated analysis uncovered 381 Gene-CpG pairs. GABAA receptor genes and related pathways, the neuroactive ligand receptor interaction pathway, ABCD1/2 gene activity, and cell signalling processes were overrepresented, providing insight into the underlying biological mechanisms facilitating these associations. We also report evidence implicating chromatin state regulatory mechanisms as modulators of mtDNA-CN effect on gene expression. We demonstrate that mitochondrial DNA variation signals to the nuclear DNA epigenome and transcriptome and may lead to nuclear remodelling relevant to development, aging, and complex disease.
OGT prevents DNA demethylation and suppresses the expression of transposable elements in heterochromatin by restraining TET activity genome-wide

The O-GlcNAc transferase OGT interacts robustly with all three mammalian TET methylcytosine dioxygenases. We show here that deletion of the Ogt gene in mouse embryonic stem cells (mESC) results in a widespread increase in the TET product 5-hydroxymethylcytosine (5hmC) in both euchromatic and heterochromatic compartments, with concomitant reduction of the TET substrate 5-methylcytosine (5mC) at the same genomic regions. mESC engineered to abolish the TET1-OGT interaction likewise displayed a genome-wide decrease of 5mC. DNA hypomethylation in OGT-deficient cells was accompanied by de-repression of transposable elements (TEs) predominantly located in heterochromatin, and this increase in TE expression was sometimes accompanied by increased cis-expression of genes and exons located 3’ of the expressed TE. Thus, the TET-OGT interaction prevents DNA demethylation and TE expression in heterochromatin by restraining TET activity genome-wide. We suggest that OGT protects the genome against DNA hypomethylation and impaired heterochromatin integrity, preventing the aberrant increase in TE expression observed in cancer, autoimmune-inflammatory diseases, cellular senescence and ageing.
Identification, antimicrobial resistance and molecular detection of Shiga toxin-producing E. coli (STEC) isolated from raw milk of cattle in Chitwan, Nepal

Shiga toxin-producing Escherichia coli (STEC) is one of the most common foodborne pathogens leading to gastroenteritis with symptoms of mild diarrhoea to the potential life-threatening haemolytic uremic syndrome. Serotype O157:H7 has been the most predominant type worldwide but there are an increasing number of outbreaks caused by non-O157:H7 STEC serotypes. This is the first study about STEC in milk used in Nepalese communities. A total of 267 raw milk samples were taken from different sites in Chitwan District, one of the major milk-producing districts of Nepal, using multi-stage sampling. The samples underwent microbiological and molecular analysis. The overall prevalence of E. coli was found to be 29.6% (79/267) in raw cattle milk. Z3276 gene, a specific genetic marker of E. coli O157:H7 was detected only in three isolates which also carried stx genes. The overall prevalence of STEC in raw cattle milk was 11.6% (31/267) whereas the prevalence of STEC among E. coli isolates was 39.2% (31/79). The prevalence of O157:H7 STEC is 1.1% (3/267) and of non-O157:H7 STEC is 10.5% (28/267). Out of 12 antibiotics tested, the sensitivity of STEC to an antibiotic was highest with azithromycin, and levofloxacin, and the most resistant was with tetracycline, and doxycycline. The findings revealed that raw cattle milk was contaminated with STEC, posing a potential public health concern. The discovery of a greater occurrence of stx2 genes in this study highlights the possibility of more severe complications in humans. Molecular-based STEC surveillance is essential to minimize the risk of its transmission.
Preliminary Trabeculectomy results using the Moorfields Safer Surgery Technique in Malta

Purpose: To review the results of the Moorfields Safer Surgery System (MSSS) for trabeculectomy, recently introduced in Malta. Methods: Patient files were reviewed from data collected over an 18 month period, from the Maltese national teaching hospital, Mater Dei Hospital. Files of all patients undergoing primary trabeculectomy with a minimum of 12 months follow up data available were reviewed. Primary outcome measure of success was deﬁned as a 30% drop in ﬁnal post-operative intraocular pressure (IOP) at 1 year. Secondary outcome measure of success was ﬁnal post-op IOP of less than 21mmHg. Unqualified success was defined as satisfactory IOP without the need of anti-glaucoma medication, while qualified success was defined as satisfactory IOP in those patients requiring anti-glaucoma medication. Results: 43 eyes (mean age = 66.2 yrs ± 11.7) were analysed. The mean pre-operative IOP was 27.0mmHg ± 4.6. Mean post-op IOP at one year was 15.3mmHg ±2.7. Unqualified success for the primary outcome measure was achieved in 64.1% of patients while the qualified success was achieved in 82.1%. Unqualified success for the secondary outcome measure was achieved in 72.7% and a qualified success, of 94.8% was obtained. There was 6.8% failure rate. Conclusions: The results from this first review using the Moorfields safe surgery system for Trabeculectomy surgery in the Maltese islands compares well to the current literature.
The Moderating Role of Perfect Hijab (Chador) in the Relationship of Body Image Concern and Dissatisfaction with Body image with Social Anxiety among Female University Students

The present research was designed with the aim of determining the moderating role of using the perfect Hijab (chador) in the relationship among worry and dissatisfaction about body image and social anxiety.For this purpose,261 female university students of Karaj Islamic Azad University were selected through purposive sampling (86 with Perfect Hijab,87 with Conventional Hijab and 88 with Unconventional Hijab) and responded to the Liebowitz's Social Anxiety Scale and Fischer's Body Image scale and Littleton's The Body Image Concern Inventory. The measurement model of this study was processed by structural-moderator equations modeling using partial least squares using Smart PLS software.The results showed that social anxiety increased with increasing levels of worry and dissatisfaction about body image.Moderating analyses also revealed that the effect of worry and dissatisfaction about body image on social anxiety in the three groups of women with different dressing of perfect Hijab(chador), conventional Hijab (Manteau) and unconventional dressing did not differ, and therefore the dressing type did not reduce (moderate) the intensity of the relationships among independent and dependent variables at 95%confidence level. Worry and dissatisfaction with body image is associated with their social anxiety in female university students and regardless of the type of hijab, these women need psychological interventions.
The use of complementary medicine during childbearing years: A The use of complementary medicine during childbearing years: A multi-country study of women from the Middle East multi-country study of women from the Middle East

The prevalence of self-prescribed complementary medicine (CM) use is an area of concern across the Middle East. We conducted a descriptive qualitative study to describe women ’ s use of CM during their childbearingyearsinEgypt, Jordan, Saudi Arabia andIran. The study conducted using face to face audio-recorded interviews with 70 participants. Our ﬁ ndings showed that women used several herbal plants to ease pain and discomfort during menstruation and/or during pregnancy and in postpartum care. Acupuncture, meditation, and massage were lesslikelytobe usedbywomen.TheimpactofCMwasoftendescribedinrelationtoperceivedusefulnessandsafetyofherbs. Cultural beliefs and roles of women contributed to the use of herbs among women. Given the high use of herbal medicine, there is a need for discussion about the potential of adverse effects of some plants or their metabolites following self-medication during the childbearing years.
Antioxidant Activity and Mechanism of Tetrahydroamentoflavone in vitro

(2S,2″S)-Tetrahydroamentoflavone (THA) is a typical biflavonoid. In this study, we systematically evaluated its antioxidant activity by various antioxidant assays in vitro. The IC50 values of THA were 4.8±0.3, 743.2±49.5, 35.5±1.9, 165.7±22.8, 4.4±0.2, and 77.1±2.2 μg/mL, respectively, for superoxide (•O2-) radical-scavenging, Fe2+-chelating, Cu2+-chelating, DPPH• (1,1-diphenyl-2-picrylhydrazyl radical) scavenging, ABTS•+ (3-ethylbenzthiazoline-6-sulfonic acid radical) scavenging, and Cu2+-reducing power. The average ratio value of IC50, Trolox:IC50, THA was calculated as 2.54, suggesting that THA possesses 2.54 times the total antioxidant level than the standard antioxidant Trolox. THA exerts its antioxidant activity in vitro through metal-chelating, and radical-scavenging, which is via donating a hydrogen atom (H•) and an electron (e). Its antioxidant activity can be responsible for its pharmacological effect and make it an attractive natural antioxidant.
Patient Attachment and Reflective Functioning as Predictors for Therapist Countertransference

Therapists’ reactions towards patients are important for the therapeutic process, and is influenced by therapist and patient characteristics. This study aimed to improve the understanding of therapists’ emotional reactions by investigating if patients’ attachment and ability to mentalize predicted therapist countertransference in psychotherapy. Multilevel modeling was used to analyse 87 therapy-dyads in psychotherapy. Patient attachment, measured pre-treatment using self-reports, and mentalization operationalized as Reflective functioning (RF) were hypothesized to predict therapist self-reported emotional experiences (conceptualized as countertransference) over the complete treatment and in different treatment phases. Results showed that lower patient RF predicted more negative therapist countertransference in complete treatment. In the middle and final phases of therapy, lower RF predicted more negative countertransference, and higher RF predicted more positive countertransference. Higher level of avoidant attachment in patients predicted more relaxed therapist countertransference in the final phase of treatment. Findings indicate that patients’ mentalizing ability is important to consider in psychotherapy, as it has informational value for the therapeutic process.
COMPUTABLY AND PUNCTUALLY UNIVERSAL SPACES

We prove that the standard computable presentation of the space C [0 , 1] of continuous real-valued functions on the unit interval is computably and punctually (primitively recursively) universal. From the perspective of modern computability theory, this settles a problem raised by Sierpi´nski in the 1940s. Weprove that the original Urysohn’s construction of the universal separable Polish space U is punctually universal. We also show that eﬀectively compact, punctual Stone spaces are punctually homeomorphically embeddable into Cantor space 2 ω ; note that we do not require eﬀective compactness be primitive recursive. We also prove that eﬀective compactness cannot be dropped from the premises by constructing a counterexample.
Critical Success Factors for Data Governance: A Theory Building Approach

ABSTRACT The objective of this research study is to present the critical success factors (CSFs) for data governance (DG). This paper reports on a single case study where data are gathered through semi-structured interviews following the CSF approach and analyzed by applying open, axial, and selective coding techniques. The findings are presented as seven CSFs, which are ranked in order of importance. Furthermore, we highlight the need to better understand the relationships (interconnectedness) between the CSFs.
제도적 거리와 해외직접투자의 관계에 관한 연구: 중국을 중심으로* A Study on the Relationship Between Institutional Distance and Outward Foreign Direct Investment: the Case of China

This study aims to investigate the relationship between institutional distance and FDI and focuses on China's outward FDI. The institutional distance between China and the host country is measured using the institutional quality published by the World Bank. This study collects panel data from 50 countries in which China invested from 2008 to 2019 and use the panel GLS methodology to examine the factors affecting outward FDI through three models. First, this study examines the impact of the absolute value of institutional distance on China's OFDI across all countries in which China invests. Second, this study divides countries with positive and negative institutional distance to China into two groups and examine the relationship between institutional distance and OFDI in each group. Finally, this study examines the non-linear relationship between institutional distance and OFDI from China. To test this, this study adds the squared term of institutional distance to the model. The results of the analysis are as follows Institutional distance is positively related to China's OFDI. The relationship between institutional distance and OFDI is inverted U-shaped in the group of host countries with relatively higher institutional quality than China, but positive in the group of low-quality host countries. In addition, China's OFDI is affected by a combination of institutional and economic factors. The results of this study have implications not only for FDI host countries but also for MNCs' choice of FDI destinations.
Turn-to-Turn Converter Stress Impact on the Lifetime of Rotating Machines

The effects of turn-to-turn converter stresses on the lifetime of Litz wires samples have been investigated. Three-level low duty waveshapes have been used to produce a voltage simulating the one appearing during operation. Under such waveform a higher partial discharge inception voltage was recorded. However, no significant improvement in endurance times under discharge was observed, likely due to PD events occurring predominantly at the waveform flanks in converter operation. Corona resistant materials can only partially alleviate the issue, hence PD-free design approaches may be required in many circumstances.
Origin and transportation history of lunar breccia 14311

In this paper, we compare the U‐Pb zircon age distribution pattern of sample 14311 from the Apollo 14 landing site with those from other breccias collected at the same landing site. Zircons in breccia 14311 show major age peaks at 4340 and 4240 Ma and small peaks at 4110, 4030, and 3960 Ma. The zircon age patterns of breccia 14311 and other Apollo 14 breccias are statistically different suggesting a separate provenance and transportation history for these breccias. This interpretation is supported by different U‐Pb Ca‐phosphate and exposure ages for breccia 14311 (Ca‐phosphate age: 3938 ± 4 Ma, exposure age: ~550–660 Ma) from the other Apollo 14 breccias (Ca‐phosphate age: 3927 ± 2 Ma, compatible with the Imbrium impact, exposure age: ~25–30 Ma). Based on these observations, we consider two hypotheses for the origin and transportation history of sample 14311. (1) Breccia 14311 was formed in the Procellarum KREEP terrane by a 3938 Ma‐old impact and deposited near the future site of the Imbrium basin. The breccia was integrated into the Fra Mauro Formation during the deposition of the Imbrium impact ejecta at 3927 Ma. The zircons were annealed by mare basalt flooding at 3400 Ma at Apollo 14 landing site. Eventually, at approximately 660 Ma, a small and local impact event excavated this sample and it has been at the surface of the Moon since this time. (2) Breccia 14311 was formed by a 3938 Ma‐old impact. The location of the sample is not known at that time but at 3400 Ma, it was located nearby or buried by hot basaltic flows. It was transported from where it was deposited to the Apollo 14 landing site by an impact at approximately 660 Ma, possibly related to the formation of the Copernicus crater and has remained at the surface of the Moon since this event. This latter hypothesis is the simplest scenario for the formation and transportation history of the 14311 breccia.
Product-Matrix Construction of Minimum Storage Rack-aware Regenerating Codes

In data centers, storage nodes are typically organized in racks and the cross-rack communication cost is often much higher than the intra-rack communication cost. Minimum Storage Rack-aware Regenerating (MSRR) codes are special maximum distance separable (MDS) array codes that can achieve the minimum cross-rack repair bandwidth for single node failures. In this paper, we present an explicit construction of MSRR codes by employing the product-matrix framework. Our product-matrix MSRR codes have lower sub-packetization level than the existing MSRR codes.
Pairwise Preference over Multi-type Implicit Feedback Confidence Based Bayesian Personalized Ranking for Collaborative Filtering

Implicit feedback (e.g., purchases, views, clicks) have been receiving more attention due to their close relationship with real applications, including shopping, making friends, healthcare, etc. In some recent works, pairwise ranking algorithms have been shown to leverage different types of users' behaviors to solve the recommendation problem of heterogeneous implicit feedback. The ever-growing healthcare websites impose a new challenge for medical services recommendation. In this paper, we develop a novel preference learning algorithm to learn a confidence over multiple users' actions, which is called Multi-type Implicit Feedback Confidence Learned Bayesian Personalized Ranking (MTC-BPR). MTC-BPR has the merits of generating positive data from multiple auxiliary feedback and accommodates both the original and generated data for pairwise ranking. Experimental results on the real-world dataset from a healthcare website demonstrate that MTC-BPR achieves more accurate recommendations than several state-of-the-art methods.