Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction of survival-related co-expression modules and identification of potential prognostic biomarkers of osteosarcoma using WGCNA

Background Osteosarcoma (OS) is a primary malignant bone tumor. Patients with different immune characteristics respond differently to chemotherapy and have a lower chance of survival. The potential pathogenesis and therapeutic targets of OS must be investigated further. Methods OS expression profile data and clinical information were downloaded from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and the Gene Expression Omnibus (GEO) databases. The immune-related gene set was obtained from the ImmPort database, and the immune-related gene expression profiles were used for non-negative matrix factorization (NMF) cluster analysis of patients in the 2 databases to find the best clustering number. In the TARGET database, OS patients were classified into low-risk and high-risk groups based on the differences in their survival rates. Weighted correlation network analysis (WGCNA) was applied to the low-risk and high-risk groups to determine the module with the lowest conservatism in order to differentiate the prognosis of the 2 groups. Results A total of 500 key genes associated with poor prognosis were identified. Gene Ontology (GO) enrichment analysis revealed that the biological processes of these genes were primarily focused on the regulation of small guanosine triphosphatase (GTPase) mediated signal transduction, collagen-containing extracellular matrix, and Rho GTPase binding. A random survival forest identified EPHB3, TEAD1, and KRR1P1 as key genes. Their expression level was linked to overall survival. We discovered that the core genes were associated to immune cell infiltration. Simultaneously, paired survival analysis of two genes revealed differences in survival. We also reverse-predicted the main genes and developed their competitive endogenous RNA (ceRNA) network. Finally, utilizing the CellMiner database, we observed that the genes TEAD1 and EPHB3 were connected to drug sensitivity. Conclusions In this study, we identified the modules and key genes related to the poor prognosis of OS patients by using WGCNA, and verified their impact on the prognosis of OS patients and their role in the immune microenvironment of OS. In addition, targeted gene related antitumor drugs were screened out. The discoveries may lead to novel molecular targets and treatment methods for OS patients. Keywords Osteosarcoma (OS); weighted gene co-expression network analysis (WGCNA); gene
PREDICTORS OF PAIRED, SINGULAR, AND NO ACTION AMONG MIDDLE SCHOOL STUDENTS WITH BEHAVIOR RISKS

Understanding multiple health risk behavior change may contribute to the prevention of chronic illness. The present study examined drivers of behavior change over time within individuals with health behavior risks who were at risk for two behaviors. Participants were middle school students from a randomized controlled trial conducted across 20 middle schools within the state of Rhode Island using two transtheoretical model-tailored, computer-delivered interventions in the school setting. Participants received an alcohol prevention program or an energy balance program. Analyses were conducted with participants who were at risk for both physical activity and fruit and vegetable intake (N = 1401). A series of chi-square analyses, one-way between subjects ANOVAs, and MANOVA models were conducted to examine whether (1) Treatment (received energy balance intervention), (2) baseline Stage of Change, (3) Effort, and (4) Severity predicted the number of behaviors that an individual changes (no behaviors, one behavior, or two behaviors) at three follow-up time points (12, 24, and 36 months). Additionally, demographic effects were examined in the context of participants at risk for multiple health behaviors. Treatment, Stage of Change, Severity, and Effort at baseline, were all significantly related to the number of behaviors changed at a certain time point over the course of the study12, 24, or 36 months, or across multiple time points. Treatment demonstrated the greatest consistency across behaviors and time points, with more participants in the treatment condition being in the paired action group (change on two behaviors) at each time point (12, 24, and 36 months). Furthermore, age, was the only consistent demographic effect across time points. Findings shed light on the mechanisms of multiple behavior change within individuals, providing a deeper understanding of what impacts the number of behaviors that an individual changes over time.
Unguided Computer-Assisted Self-Help Interventions Without Human Contact in Patients With Obsessive-Compulsive Disorder: Systematic Review and Meta-analysis

Background Computer-assisted treatment may reduce therapist contact and costs and promote client participation. This meta-analysis examined the efficacy and acceptability of an unguided computer-assisted therapy in patients with obsessive-compulsive disorder (OCD) compared with a waiting list or attention placebo. Objective This study aimed to evaluate the effectiveness and adherence of computer-assisted self-help treatment without human contact in patients with OCD using a systematic review and meta-analysis approach. Methods Randomized controlled trials with participants primarily diagnosed with OCD by health professionals with clinically significant OCD symptoms as measured with validated scales were included. The interventions included self-help treatment through the internet, computers, and smartphones. We excluded interventions that used human contact. We conducted a search on PubMed, Cochrane Central Register of Controlled Trials, EMBASE, World Health Organization International Clinical Trials Registry Platform, and ClinicalTrials.gov, as well as the reference lists of the included studies. The risk of bias was evaluated using version 2 of the Cochrane risk-of-bias tool for randomized trials. We calculated the standardized mean differences for continuous outcomes and risk ratios for dichotomous outcomes. The primary outcomes were short-term improvement of OCD symptoms measured by validated scales and dropout for any reason. Results We included 11 randomized controlled trials with a total of 983 participants. The results indicated that unguided computer-assisted self-help therapy was significantly more effective than a waiting list or psychological placebo (standard mean difference −0.47, 95% CI −0.73 to −0.22). Unguided computer-assisted self-help therapy had more dropouts for any reason than waiting list or psychological placebo (risk ratio 1.98, 95% CI 1.21 to 3.23). However, the quality of evidence was very low because of the risk of bias and inconsistent results among the included studies. The subgroup analysis showed that exposure response and prevention and an intervention duration of more than 4 weeks strengthen the efficacy without worsening acceptability. Only a few studies have examined the interaction between participants and systems, and no study has used gamification. Most researchers only used text-based interventions, and no study has used a mobile device. The overall risk of bias of the included studies was high and the heterogeneity of results was moderate to considerable. Conclusions Unguided computer-assisted self-help therapy for OCD is effective compared with waiting lists or psychological placebo. An exposure response and prevention component and intervention duration of more than 4 weeks may strengthen the efficacy without worsening the acceptability of the therapy. Trial Registration PROSPERO (International Prospective Register of Systematic Reviews) CRD42021264644; https://www.crd. york.ac. uk/prospero/display_record. php? RecordID=264644
Golden codeword‐based generalized spatial modulation

Conventional‐generalized spatial modulation (C‐GSM) is an NT×NR multiple‐input multiple‐output (MIMO) spatial modulation (SM) scheme that transmits the same complex amplitude/phase modulation symbol from multiple active transmit antennas. By using two active transmit antennas during a transmission interval, C‐GSM is capable of achieving high spectral efficiencies. However, as the size of the signal constellation/domain increases, the minimum Euclidean distances (ED) between the symbols decrease. Hence, this leads to a degradation of the average bit error rate (ABER) performance of the scheme. Hence, motivated to improve the error performance of C‐GSM schemes, first, this study proposes a new scheme called Golden codeword‐based generalized spatial modulation (GCW‐GSM), which combines C‐GSM and the Golden code to take advantage of the benefits of both while avoiding their drawbacks. The proposed scheme uses conventional quadrature amplitude modulation (C‐QAM), and it is investigated over Rayleigh frequency‐flat fading channels with additive white Gaussian noise. Second, a closed‐form expression of the theoretical average bit error probability of the proposed GCW‐GSM scheme is formulated. Compared to simulation results, it proves to be increasingly tight at high signal‐to‐noise ratio values. Finally, the proposed scheme performance over the Rayleigh‐frequency‐flat fading channel is compared to that over the Rician channel. An improvement in the error performance of 6dB with spectral efficiency of 10bits/s/Hz is seen in 4×4 C‐16QAM GCW‐GSM over 4×4 C‐256QAM conventional‐spatial modulation and 4×4 C‐256QAM C‐GSM schemes at a bit error rate (BER) of 1×10−5 . The 4×4 C‐16QAM GCW‐GSM scheme exhibits an improvement in the error performance of 2.9dB with spectral efficiency of 10bits/s/Hz over the 4×4 C‐64QAM conventional quadrature spatial modulation scheme at a BER of 1×10−5 . Finally, the 4×4 16‐QAM GCW‐GSM under Rayleigh fading has a performance gain of 3 dB over 4×4 16‐QAM GCW‐GSM under Rician fading with a Rician K factor of 3.
The size and sternal involvement of chest wall resections for malignant disease predict postoperative morbidity

Background Chest wall resections/reconstructions are a validated approach to manage tumors invading the thorax. However, how resection characteristics affect postoperative morbidity and mortality is unknown. We determined the impact of chest wall resection size and location on patient short and long-term postoperative outcomes. Methods We reviewed all consecutive patients who underwent resections/reconstructions for chest wall tumors between 2003 and 2018. The impact of chest wall resection size and location and reconstruction on perioperative morbidity/mortality and oncological outcome were evaluated for each patient. Results Ninety-three chest wall resections were performed in 88 patients for primary (sarcoma, breast cancer, n=66, 71%) and metastatic (n=27, 29%) chest wall tumors. The mean chest bony resection size was 107 (range, 15–375) cm2 and involved ribs only in 57% (n=53) or ribs combined to sternal/clavicular resections in 43% of patients (n=40). Chest defect reconstruction methods included muscle flaps alone (14%) prosthetic material alone (25%) or a combination of both (61%). Early systemic postoperative complications included pneumonia (n=15, 16%), atelectasis (n=6, 6%), pleural effusion (n=15, 16%) and arrhythmia (n=6, 6%). The most frequent long-term reconstructive complications included wound dehiscence (n=4), mesh infection (n=5) and seroma (n=4). Uni- and multivariable analyses indicated that chest wall resection size (>114 cm2) and location (sternum) were significantly associated with the occurrence of pneumonia and atelectasis [odds ratio (OR) =3.67, P=0.05; OR =78.92, P=0.02, respectively]. Disease-free and overall survival were 37±43 and 48±42 months for primary malignancy and of 24±33 and 48±53 months for metastatic chest wall tumors respectively with a mean follow-up of 46±44 months. Conclusions Chest wall resections present good long-term oncological outcomes. A resection size above 114 cm2 and the involvement of the sternum are significantly associated with higher rates of postoperative pneumonia/atelectasis. This subgroup of patients should have reinforced perioperative physical therapy protocols.
Multidimensional Family-Centred Early Intervention in Children with Hearing Loss: A Conceptual Model

At least two per thousand newborns are affected by hearing loss, with up to 40% with an additional disability. Early identification by universal newborn hearing screening and early intervention services are available in many countries around the world, with limited data on their effectiveness and a lack of knowledge about specific intervention-related determinants of child and family outcomes. This concept paper aimed to better understand the mechanisms by which multi-dimensional family-centred early intervention influences child outcomes, through parent behaviour, targeted by intervention by a review of the literature, primarily in the field of childhood hearing loss, supplemented by research findings on physiological and atypical child development. We present a conceptual model of influences of multi-disciplinary family-centred early intervention on family coping/functioning and parent–child interaction, with effects on child psycho-social and cognitive outcomes. Social communication and language skills are postulated as mediators between parent–child interaction and non-verbal child outcomes. Multi-disciplinary networks of professionals trained in family-centred practice and the evaluation of existing services, with respect to best practice guidelines for family-centred early intervention, are recommended. There is a need for longitudinal epidemiological studies, including specific intervention measures, family behaviours and multidimensional child outcomes.
Assisted reproductive technologies in Latin America: the Latin American Registry, 2019

Research question What was the utilization, effectiveness and safety of assisted reproductive technology (ART) in Latin America during 2019? Design This was a retrospective collection of multinational data on ART performed at 196 institutions from 15 countries. Results A total of 106,918 initiated cycles, 18,133 deliveries and 21,096 births were reported. ART utilization was 24-558 cycles per million inhabitants. Women aged ≥40 years represented 32.9% of fresh IVF and intracytoplasmic sperm injection (ICSI) cycles. After removing freeze-all cycles, the delivery rate per oocyte retrieval was 17.3% for ICSI and 19.5% for IVF. Overall, single-embryo transfer (SET) represented 36.2% of fresh transfers, with a 19.5% delivery rate per transfer, increasing to 30.7% for elective SET and 32.7% for blastocyst elective SET (eSET). The delivery rate for double-embryo transfers (DET) was 27.8%, increasing to 37.1% after elective DET. This 6.4% increment in deliveries between eSET and elective DET resulted in a 12-fold increase in twin births. Furthermore, overall perinatal mortality was more than two-fold higher for twin compared with singleton deliveries. The delivery rate for frozen-thawed SET reached 28.1%, most being blastocyst transfers. Of all births, 72.3% were singletons, 26.4% twins and 1.3% triplets and higher multiples. Preterm deliveries reached 14.3% for singletons and 58.1% for twins. Perinatal mortality was 7.4‰ in singletons, 17.2‰ for twins and 62.9‰ for triplets or higher. Conclusions The number of initiated cycles has slowly increased in countries with laws or regulations facilitating access. FET cycles predominate and blastocyst SET are also increasing. The data show that, especially in young women and oocyte recipients, when there is more than one blastocyst for transfer, eSET should be the rule.
Rutile to Titanite Transformation in Eclogites and its Geochemical Consequences: An Example from the Sumdo Eclogite, Tibet

The formation of titanite coronae after rutile is common in retrograde high‐ to ultrahigh‐pressure meta‐mafic rocks, which provides a good opportunity to address the geochemical behavior of HFSE in crustal environments. In the Sumdo eclogite, titanite occurs either as a corona around rutile grains or as semi‐continuous veins cross‐cutting the major foliation, whereas rutile grains occur either as inclusions in garnet or omphacite or as a relict core surrounded by titanite. Textural relationships and trace elements characteristics of rutile and titanite with different occurrences indicate that both minerals preferentially incorporate Nb and Hf over Ta and Zr in aqueous fluid. Moreover, the breakdown of omphacite and epidote could release substantial amounts of aqueous fluids enriched in Ca, Si, Fe and REE, which would react with rutile to form titanite coronae and veins. During this process, water‐insoluble elements, like HFSE and HREE, behave like mobile elements, but they do not migrate substantially out of the system, instead, tending to react in situ. This suggests that the aqueous fluids released during the retrograde metamorphic reactions in mafic rocks could not substantially transfer fluid‐immobile elements into the overlying mantle wedge in subduction environments.
The Emergence of HHV-8 Infection among HIV-positive Individuals Residing in Bali, Indonesia

Background: Human herpesvirus 8 (HHV-8) is considered as the etiological agent of Kaposi’s sarcoma (KS). In people living with human immunodeficiency virus (HIV) (PLHIV), KS defines acquired immunodeficiency syndrome (AIDS). Previous study found that HIV-positive individuals were more likely to be HHV-8 seropositive than HIV-negative individuals. Reports regarding the presence of HHV-8 and HIV co-infection in Indonesia is severely limited. Aim: This study aimed to identify HHV-8 infection among HIV-positive individuals in Bali, Indonesia. Materials and Methods: Forty three plasma samples were collected from 16 antiretroviral therapy (ART) naive and 27 ART-experienced individuals residing in Buleleng Regency, Bali, Indonesia. Detection of HHV-8 antigen was performed by using ELISA method. The Fisher's Exact test and the Spearman correlation test would be used to analysed data accordingly. Results: Seven samples (7/43, 16.3%), one of ART-naive individual (1/16, 6.25%) and six of ART-experienced individuals (6/27, 22.2%), were tested positive for HHV-8 antigen. Human herpesvirus 8 infection was correlated neither to sex (p=1.000) and age (p=0.716), nor to ART status (p=0.178) and length of ART (p=0.465). Conclusion: This study indicates the emergence of HHV-8 infection among HIV-positive individuals residing in Bali, Indonesia. Sufficient diagnosis of HHV-8 should be considered for all HIV-positive individuals in order to deliver appropriate treatment. Extensive researches is suggested to be conducted in more regions of Indonesia in order to determine the magnitude of HHV-8 infection among HIV-positive individuals.
Anxiety and Depression among Hypertensive Adults in Tertiary Care Hospitals of Nepal

Introduction Cooccurrence of hypertension and depression/anxiety increases the chance of cardiovascular mortality and morbidity. Therefore, this study is aimed at assessing the prevalence of anxiety and depression and their association with hypertension among hypertensive adults in a tertiary care hospital in Kathmandu, Nepal. Methods A descriptive cross-sectional study was conducted using a semistructured self-administered questionnaire based on Hamilton Anxiety and Hamilton Depression Rating Scale. The data was entered in EPI Data and analyzed using descriptive and inferential statistics in SPSS version 22. P value < 0.05 was considered statistically significant. Results A total of 260 individuals participated in the study, with a mean age of 42.6 years. About 46% of patients did not have any symptoms of depressed mood, and 73 (28.1%) of the participants experienced feelings of depressed mood only on questioning. Similarly, (151) 58.1% did not have feelings of guilt, and 48 (18.5%) participants who had the feeling of guilt had let people down. Among 260 respondents, most participants ((102) 39.2%) had mild symptoms of anxious mood, followed by (86) 33.1% participants with moderate symptoms. Only (4) 1.5% of participants had severe symptoms. Similarly, the majority of participants ((114) 43.8%) had a mild form of mental and emotional strain, followed by (72) 27.7% with moderate mental and emotional strain while (43) 16.5% had no mental and emotional strain. The occupation and marital status of the hypertensive individual was associated with anxiety and depression (P = ≤0.01). Conclusion In conclusion, anxiety and depression were common among patients with hypertension. Anxiety and depression were linked to some of the patients' sociodemographic and clinical features. This study demonstrates that treating hypertension alone is not enough to improve patients' quality of life; mental illness screening among chronically ill individuals is also required.
Tackling the Crowdsourced Delivery Problem at Scale through a Set-Partitioning Formulation and Novel Decomposition Heuristic

This paper presents a set-partitioning formulation and a novel decomposition heuristic (D-H) solution algorithm to solve large-scale instances of the urban crowdsourced shared-trip package delivery problem. The D-H begins by dividing the packages between shared personal vehicles (SPVs) and dedicated vehicles (DVs). For package-assignment to SPVs, this paper enumerates the set of routes each SPV can traverse and constructs a package-SPV route assignment problem. For package-assignment to DVs and routing, the paper first obtains DV routes by solving a conventional vehicle routing problem and then seeks potential solution improvements by switching packages from SPVs to DVs. The switching process is cost driven. The D-H significantly outperforms a commercial solver in terms of computational efficiency, while obtaining near-optimal solutions for small problem instances. This paper presents a city-scale case study to analyze the important service design factors that impact the efficiency of crowdsourced shared-trip delivery. The paper further analyzes the impact of three important service design factors on system performance, namely (i) the number of participating SPVs, (ii) the maximum detour willingness of SPVs, and (iii) the depot locations. The results and findings provide meaningful insights for industry practice, while the algorithms illustrate promise for large real-world systems.
A Review of In Vivo and Clinical Studies Applying Scaffolds and Cell Sheet Technology for Periodontal Ligament Regeneration

Different approaches to develop engineered scaffolds for periodontal tissues regeneration have been proposed. In this review, innovations in stem cell technology and scaffolds engineering focused primarily on Periodontal Ligament (PDL) regeneration are discussed and analyzed based on results from pre-clinical in vivo studies and clinical trials. Most of those developments include the use of polymeric materials with different patterning and surface nanotopography and printing of complex and sophisticated multiphasic composite scaffolds with different compartments to accomodate for the different periodontal tissues’ architecture. Despite the increased effort in producing these scaffolds and their undoubtable efficiency to guide and support tissue regeneration, appropriate source of cells is also needed to provide new tissue formation and various biological and mechanochemical cues from the Extraccellular Matrix (ECM) to provide biophysical stimuli for cell growth and differentiation. Cell sheet engineering is a novel promising technique that allows obtaining cells in a sheet format while preserving ECM components. The right combination of those factors has not been discovered yet and efforts are still needed to ameliorate regenerative outcomes towards the functional organisation of the developed tissues.
Qualitative properties of nonlocal discrete operators

In this article, we prove new convexity results for nonlocal discrete operators across an entire region that covers sequential orders in two parameters. We review and extend current studies on the properties of positivity, monotonicity, and convexity, explore borderline cases, and provide new insights on such properties by means of original examples evidencing the sharpness of the results. Our method is based on the novel principle of transference.