Miracle-dz/UniFAR-FTUs huggingface dataset:
Complete Genome Sequence of Lactococcus lactis subsp. lactis G121, an Isolate with Allergy-Protective Features Derived from a Farming Environment

Early childhood exposure to a farming environment has been found to be protective against asthma and other atopic disorders. Here, we report the complete genome sequence of Lactococcus lactis subsp. lactis G121, which was isolated from the kitchen of a farm in Bavaria (Germany) and is recognized for its allergy-protective properties. It could be assembled into one circular chromosome, three circular plasmids, and one linear plasmid. ABSTRACT Early childhood exposure to a farming environment has been found to be protective against asthma and other atopic disorders. Here, we report the complete genome sequence of Lactococcus lactis subsp. lactis G121, which was isolated from the kitchen of a farm in Bavaria (Germany) and is recognized for its allergy-protective properties. It could be assembled into one circular chromosome, three circular plasmids, and one linear plasmid.
Myeloid-Derived Suppressor Cells in Pregnancy and the Neonatal Period

During pregnancy, the immune systems of mother and offspring are challenged by their close adjacency to balance tolerance and rejection. After birth the neonate has to continue this balance towards its new environment by tolerating commensals while rejecting pathogens and towards its developing tissues to avoid inflammatory damage while overcoming immunosuppression. Our group was the first to link immunosuppressive features of myeloid derived suppressor cells (MDSC) to materno-fetal tolerance, neonatal susceptibility to infection and inflammation control. Here we summarize recent advances in this dynamic field.
Experimental investigation on the effect of ethanol on micropore structure and fluid distribution of coalbed methane reservoir

The development of coalbed methane not only ensures the supply of natural gas but also reduces the risks of coal mine accidents. The micropore structure of coalbed methane reservoir affects the seepage of coalbed methane; improvement of pore structure is one of the effective methods to enhance the efficiency of coalbed methane exploitation. In this study, low-pressure nitrogen gas adsorption, specific surface area analysis, nuclear magnetic resonance spectroscopy, and centrifugation experiment were used to evaluate the effect of ethanol on coal microscopic pore structure and fluid distribution during hydraulic fracturing. Seven coal samples were collected from the No. 3 coal seam in Zhaozhuang Mine, Qinshui Basin. The samples are mainly composed of micropores, transition pores, and mesopores. The experimental results show that ethanol can significantly change the pore structure by increasing the pore diameter. The average specific surface area, pore volume, and pore diameter of rock samples before ethanol immersion are 1.1270 m2/g, 0.0104 cm3/g, and 14.20 nm, respectively. The three parameters of rock samples after ethanol immersion are 0.5865 m2/g, 0.0025 cm3/g, and 29.37 nm. Ethanol improves the connectivity between micropores and mesopores. The average irreducible fluid saturation of samples saturated with formation water after centrifugation is 86%, and the average irreducible fluid saturation of samples soaked in three concentrations of ethanol solution decreases. It is considered that an ethanol solution of 0.4% concentration has the best effect on improving the pore structure and fluid distribution.
Risk Evaluation of EMT and Inflammation in Metastatic Pancreatic Cancer Cells Following Plasma Treatment

The requirements for new technologies to serve as anticancer agents go far beyond their toxicity potential. Novel applications also need to be safe on a molecular and patient level. In a broader sense, this also relates to cancer metastasis and inflammation. In a previous study, the toxicity of an atmospheric pressure argon plasma jet in four human pancreatic cancer cell lines was confirmed and plasma treatment did not promote metastasis in vitro and in ovo. Here, these results are extended by additional types of analysis and new models to validate and define on a molecular level the changes related to metastatic processes in pancreatic cancer cells following plasma treatment in vitro and in ovo. In solid tumors that were grown on the chorion-allantois membrane of fertilized chicken eggs (TUM-CAM), plasma treatment induced modest to profound apoptosis in the tissues. This, however, was not associated with a change in the expression levels of adhesion molecules, as shown using immunofluorescence of ultrathin tissue sections. Culturing of the cells detached from these solid tumors for 6d revealed a similar or smaller total growth area and expression of ZEB1, a transcription factor associated with cancer metastasis, in the plasma-treated pancreatic cancer tissues. Analysis of in vitro and in ovo supernatants of 13 different cytokines and chemokines revealed cell line-specific effects of the plasma treatment but a noticeable increase of, e.g., growth-promoting interleukin 10 was not observed. Moreover, markers of epithelial-to-mesenchymal transition (EMT), a metastasis-promoting cellular program, were investigated. Plasma-treated pancreatic cancer cells did not present an EMT-profile. Finally, a realistic 3D tumor spheroid co-culture model with pancreatic stellate cells was employed, and the invasive properties in a gel-like cellular matrix were investigated. Tumor outgrowth and spread was similar or decreased in the plasma conditions. Altogether, these results provide valuable insights into the effect of plasma treatment on metastasis-related properties of cancer cells and did not suggest EMT-promoting effects of this novel cancer therapy.
Illuminating the Complexity of Oral Reading Fluency: A Multiple Lens Approach

Oral reading fluency is recognized as an important variable associated with the assessment of reading proficiency. Frequently, fluency assessments distill a child’s overall reading fluency to a single numerical score. Even when multiple dimensions are considered, the processes informing the nuance of the reading fluency variations are ignored. In order to shed light on the issue, we use an instrumental case study approach to illuminate how a reader’s fluency varies within a passage. We describe the insight this variability provides to teachers and researchers interested in understanding how readers transact with print. Specifically, we apply a multiple lens approach to analyze one child’s oral reading fluency and address the question: What variables contribute to a reader’s fluency? We combine eye movement miscue analysis and Flurkey’s oral reading flow and compare these assessments to Rasinski’s well-established fluency instrument. This methodology led to the identification of six variables influencing oral reading fluency. Five of the influential factors could not be observed using the traditional fluency measure. These five variables illuminate the complexity of oral reading fluency and support greater understanding of a reader’s abilities. This strengths-based conceptualization of fluency offers supportive rather than subtractive insight into a reader’s fluctuations in oral reading fluency. Our findings highlight a need for professionals to more adequately consider the conceptualizations of oral reading fluency.
Sensitivity of Photosystems II to NaCl in Relation to Salinity Tolerance. Comparative Studies With Thylakoids of the Salt Tolerant Mangrove, Avicennia marina, and the Salt-Sensitive Pea, Pisum sativum

The sensitivity of photosystem II to NaCl was compared in thylakoids isolated from the salt-tolerant mangrove, Avicennia marina, and the salt-sensitive pea, Pisum sativum. There were no indications of fundamental differences in photosystem II between these two species. Rates of oxygen evolution declined linearly with increase in NaCl from 10 to 500 mol m-3, with both species being equally sensitive. The NaCl-induced changes in Chl a fluorescence characteristics of intact thylakoids were substantially reversed by addition of hydroxylamine, indicating that the water-oxidizing site of photosystem II is sensitive to the NaCl concentration. These results are consistent with NaCl-induced depletion of the 23 and 17 kDa proteins from photosystem II-enriched membrane sheets. While the inhibition of oxygen-evolving activity by 500 mol m-3 NaCl was substantially reversed in thylakoids kept in the dark, 500 mol m-3 NaCl induced marked photoinhibitory damage in illuminated thylakoids. Thus, accumulation of ions in the chloroplasts of either salt-tolerant or salt-sensitive species would probably result in rapid damage to photosystem II, particularly in the light.
Impact of Psychotropic Medication Effects on Obesity and the Metabolic Syndrome in People With Serious Mental Illness

People with serious mental illness (SMI), including schizophrenia, bipolar disorder, and major depressive disorder, have a higher mortality rate and shortened life expectancy. This is mainly attributable to physical diseases, particularly cardiovascular diseases (CVDs). Important risk factors for CVDs are obesity and other metabolic abnormalities, which are especially prevalent in people with SMI. Several factors contribute to this increased risk, including unhealthy lifestyles. Psychotropic medication independently further increases this risk. In this review we want to examine the relationship between obesity and other components of the metabolic syndrome and psychotropic medication in people with SMI.
Presidential Delegation to Foreign Ministries: A Study of Argentina, Brazil, and Mexico (1946–2015)

When do presidents delegate policy-making authority to their foreign ministries? And is foreign policy unique in this respect? We posit that six international, national, and personal factors determine the opportunity and motivation of presidents to delegate, and then analyse the cases of Argentina, Brazil, and Mexico in 1946–2015. By applying fuzzy-set qualitative comparative analysis, we find that four combinations of factors are sufficient paths to delegation: (1) international stability and elite consensus on foreign policy; (2) international stability, right-wing president, and low diplomatic professionalisation; (3) international stability, right-wing president, and low presidential expertise on foreign policy; or (4) absence of authoritarianism combined with elite consensus on foreign policy and right-wing president. Our study of foreign ministries reinforces some of the main findings of the scholarly literature on other ministries, thus challenging the view of foreign policy-making as different from domestic policy areas.
Improved Separation of Odor Responses in Granule Cells of the Olfactory Bulb During Odor Discrimination Learning

In the olfactory bulb, olfactory information is translated into ensemble representations by mitral/tufted cells, and these representations change dynamically in a context-dependent manner. In particular, odor representations in mitral/tufted cells display pattern separation during odor discrimination learning. Although granule cells provide major inhibitory input to mitral/tufted cells and play an important role in pattern separation and olfactory learning, the dynamics of odor responses in granule cells during odor discrimination learning remain largely unknown. Here, we studied odor responses in granule cells of the olfactory bulb using fiber photometry recordings in awake behaving mice. We found that odors evoked reliable, excitatory responses in the granule cell population. Intriguingly, during odor discrimination learning, odor responses in granule cells exhibited improved separation and contained information about odor value. In conclusion, we show that granule cells in the olfactory bulb display learning-related plasticity, suggesting that they may mediate pattern separation in mitral/tufted cells.
Acute Necrotizing Encephalopathy of Childhood: A Multicenter Experience in Saudi Arabia

Background: Acute necrotizing encephalopathy of childhood (ANEC) is a rapidly progressing encephalopathy characterized by fever, depressed level of consciousness, and seizures. Diagnosis depends on clinical presentation and characteristic neuroimaging findings of abnormal signal intensity involving the thalami as well as the supra and infra-tentorial areas. Treatment modalities are not well-established; empirical treatment with antibiotics and antiviral agents is the initial step, followed by steroids and immunoglobulin, as well as supportive care. Patients with ANEC have a variable prognosis, but mortality is very high. Methods: A retrospective chart review of patients diagnosed with ANEC in five tertiary centers from January 2015 to October 2018 was performed. Clinical and radiological findings, as well as the therapeutic approach and outcomes, were described. Results: Twelve children were included ranging in age from 10 months to 6 years. All patients presented with preceding febrile illness, altered level of consciousness, and seizure. Radiological features showed abnormal signals in the thalami, and five patients (41.7%) had brainstem involvement. All patients received empirical treatment with antibiotics and antiviral agents. Ten patients (83.3%) received intravenous immunoglobulin (IVIG) and IV Methylprednisolone therapy. Outcomes were variable ranging from good outcomes with minimal neurological deficits to poor outcomes and death in 25% of cases. Conclusion: ANEC is a rare fulminant disease in children. The treatment is challenging. Early interventions with the use of IVIG and IV Methylprednisolone may change the outcome; however, further studies are needed to establish a consensus guideline for the management.
Polarity, emotions and online activity of students and tutors as features in predicting grades

The lack of physical contact and the demanding need for personalized services has prompted stakeholders in distance learning to benefit from the enormous volume of students’ online traces in the Learning Management Systems. Data mining methodologies are widely applied to analyze data logs and predict trends for early and efficient interventions. Thus, the retention of students in the educational process can be achieved with positive effects on the reputation and finances of the institutions. This work divides the moodle data sets from six different sections of an annual postgraduate program at the Hellenic Open University in six periods for each section, due to the number of written assignments. Then it implements data mining techniques to analyze the activity, polarity and emotions of tutors and students in order to predict students’ grades. The results indicate the algorithm with the highest precision in each prediction. In addition, the research concludes that polarity and emotions as independent variables provide better performance in comparative models. Moreover, tutors’ variables are highlighted as an important factor for more accurate predictions of student grades. Finally, a comparison of actual and predicted grades indicates which students have used a third party to fulfill their assignments.
Mountain linguistics

Language use in mountainous areas often exhibits special social dynamics. This contribution sketches some of the most salient patterns of language use in upland Southeast Asia ,the greater Himalayas, the Caucasus, the Central Andes, the New Guinea highlands, and touches upon the Alps and some highland areas of Africa and North America. Accumulated over long time, such patterns of use yield particular — often highly diverse, fragmented, and discontinuous — distributions of languages and language families in geographical space the precise characteristics of which also depend on prevailing local sociolinguistic and socioeconomic conditions. Generalization are still difficult since most observations are anecdotal rather than systematic: as far as language structure is concerned, languages spoken in mountainous regions are traditionally often attributed a “ conservative ” or “ archaic ” character, preserving inherited traits and patterns that are lost elsewhere; the inaccessibility of the terrain and the resulting relative social isolation are commonly invoked to explain this situation. More recently, mountain languages have also been claimed to feature peaks in the distribution of some typological properties, whether due to sociogeographical factors or, more controversially, even direct influences of the environment: ejectives, grammatical encoding of elevational or topographic information, and overall high levels of structural complexity.
Effect of hot-water treatments in vitro on conidial germination and mycelial growth of Colletotrichum musae isolates

Anthracnose caused by Colletotrichum musae is the most important postharvest disease of banana fruit. The present study was conducted to evaluate the sensitivity of C. musae isolates to hot water treatment in vitro. The effect of hot water treatment (HWT) in vitro was determined by placing conidial suspensions and plugs of agar with mycelia of 12 single spore isolates of C. musae in hot water bath at 45, 50 and 55°C for 2, 5, 8, 11, 14 and 17 min. Percent inhibition of conidial germination and colony growth increased with increased temperature and time combinations. HWT at 55°C for 5 min had an equivalent inhibition of conidial germination and reduction of mycelial growth to that of HWT at 50°C for 17 min in the 12 C. musae isolates. Remarkable variability in thermo-tolerance was found among 12 isolates of C. musae and isolate HC2 and HC3 were found to be the most sensitive, while DL1 and DL2 were comparatively heat tolerant isolates. Key words: Banana anthracnose, Colletotrichum musae, hot water treatment, sensitive, tolerant.
HIGHER INFECTION PREVALENCE IN AMPHIBIANS INHABITING HUMAN-MADE COMPARED TO NATURAL WETLANDS

Abstract: It is unclear how suitable human-made wetlands are for supporting wildlife and how they impact wildlife disease risk. Natural wetlands (those that were created without human actions) can support more diverse and resilient communities that are at lower risk of disease outbreaks. We compared frog community composition and infection with the pathogenic fungus Batrachochytrium dendrobatidis (Bd) between human-made and natural wetlands in Tippecanoe County, Indiana, US. We conducted visual encounter surveys of frog communities and quantified Bd infection prevalence at four natural and five human-made wetlands. Water parameters associated with human practices (e.g., pH, salinity) and surrounding land use were also compared across sites. We found higher Bd infection prevalence at human-made sites than at natural sites, with monthly differences showing highest infection in spring and fall, and decreasing infection with increasing water temperature. However, we found no differences between human-made and natural sites regarding amphibian community composition, water quality, or surrounding land use. Further, we found frog density increased with distance to nearest roads among both human-made and natural sites. These findings might suggest that human-made wetlands can support frog communities similar to natural wetlands, but pose a greater risk of Bd infection.
Explaining Immigrants' Worries About Ethnic Harassment: Germany, 1986–2004

What factors shape immigrants' worries about becoming targets of ethnic harassment? This is an important question to ask, but most previous studies restricted their focus to the microlevel only. By contrast, few if any studies examined the possible macrolevel antecedents driving harassment-related worries among immigrants. This study aims to help fill this gap. Focusing on a 19-years period from 1986 to 2004 in Germany, we apply multilevel regression modeling techniques to repeated cross-sectional survey data collected among immigrants of Greek, Italian, Spanish, Turkish, and (ex-) Yugoslavian origin, linked with contextual characteristics. Our central finding is that German citizens' anti-immigrant prejudice is the key driver of longitudinal differences in immigrants' harassment-related worries. This association holds net of rival variables, such as fluctuations in media attention to ethnic harassment, as well as across all immigrant groups under study. These results bring us one important step further toward a better understanding of interethnic relations between immigrants and host society members.
The Interplay Between Prolactin and Reproductive System: Focus on Uterine Pathophysiology

Over the last years, increasing evidence has focused on crucial pathogenetic role of PRL on malignant, premalignant and benign uterine diseases. Studies in animals and humans have documented that PRL receptors (PRL-Rs) are widely expressed on uterine cells and that PRL is directly synthesized by the endometrium under the stimulatory action of progesterone. Uterine PRL secretion is finely modulated by autocrine/paracrine mechanisms which do not depend on the same control factors implied in the regulation of PRL secretion from pituitary. On the other hand, PRL is synthesized also in the myometrium and directly promotes uterine smooth muscle cell growth and proliferation. Therefore, PRL and PRL-Rs appear to play an important role for the activation of signaling pathways involved in uterine cancers and preneoplastic lesions. Circulating PRL levels are reportedly increased in patients with cervical or endometrial cancers, as well as uterine premalignant lesions, and might be used as discriminative biomarker in patients with uterine cancers. Similarly, increased PRL levels have been implicated in the endometriosis-induced infertility, albeit a clear a causative role for PRL in the pathogenesis of endometriosis is yet to be demonstrated. This evidence has suggested the potential application of dopamine agonists in the therapeutic algorithm of women with malignant, premalignant and benign uterine lesions. This review focuses on the role of PRL as tumorigenic factor for uterus and the outcome of medical treatment with dopamine agonists in patients with malignant and benign uterine disease.
Bacilli-Mediated Degradation of Xenobiotic Compounds and Heavy Metals

Xenobiotic compounds are man-made compounds and widely used in dyes, drugs, pesticides, herbicides, insecticides, explosives, and other industrial chemicals. These compounds have been released into our soil and water due to anthropogenic activities and improper waste disposal practices and cause serious damage to aquatic and terrestrial ecosystems due to their toxic nature. The United States Environmental Protection Agency (USEPA) has listed several toxic substances as priority pollutants. Bacterial remediation is identified as an emerging technique to remove these substances from the environment. Many bacterial genera are actively involved in the degradation of toxic substances. Among the bacterial genera, the members of the genus Bacillus have a great potential to degrade or transform various toxic substances. Many Bacilli have been isolated and characterized by their ability to degrade or transform a wide range of compounds including both naturally occurring substances and xenobiotic compounds. This review describes the biodegradation potentials of Bacilli toward various toxic substances, including 4-chloro-2-nitrophenol, insecticides, pesticides, herbicides, explosives, drugs, polycyclic aromatic compounds, heavy metals, azo dyes, and aromatic acids. Besides, the advanced technologies used for bioremediation of environmental pollutants using Bacilli are also briefly described. This review will increase our understanding of Bacilli-mediated degradation of xenobiotic compounds and heavy metals.