Miracle-dz/UniFAR-FTUs huggingface dataset:
satisfaction survey among primary health care outpatients in the backward region: an empirical study from rural Western china

Background: With a growing health demand, patient satisfaction analysis is essential for evaluating the accessibility and performance of medical services. Previous studies had explored the Chinese outpatient satisfaction and influencing factors in developed areas and tertiary hospitals. Considering the lower education level, less income, and heavier economic burden, it was necessary to conduct a region-specific questionnaire survey for the outpatient’s satisfaction in rural Western China. Objective: To analyze the satisfaction of primary outpatient service in rural Western China, and explore the factors affecting outpatients’ satisfaction. Methods: Questionnaire composed of nine 5-Likert items was applied to survey outpatient satisfaction among randomly selected samples in 11 provinces of Western China. Exploratory factor analysis (EFA) was conducted to study the factor structure of questionnaire. Stepwise multiple linear regression analysis was performed to study the influencing factors. Results: A total of 2,754 outpatients completed the questionnaire, the response rate was 88.7%. Respondents were most satisfied with medical staff service attitude (3.71 ± 0.83) and least satisfied with medical cost (2.97 ± 0.83). A 3-factor solution was adopted in EFA to explain the overall satisfaction. Factors identified were “Service attitude”, “Facility and professional skills”, and “Patients’ cost”. And, the questionnaire was proved to have good reliability and acceptable internal consistency. The stepwise multiple linear regression analysis results presented that factors, including sample hospital type ( P , 0.05), age ( P , 0.001), education level ( P , 0.05), occupation ( P , 0.01), monthly income ( P , 0.05), and chronic disease conditions ( P , 0.01) were significantly associated with the dimensional or overall satisfaction. Conclusion: The primary health care outpatient satisfaction in rural Western China is lower than developed areas and tertiary hospitals. Care providers in backward regions should pay more attention to patients’ demographic characteristics and health status, to meet outpatients’ actual demand. Efficient hospital management methods, modern technology, and staff training are needed to improve the service quality and care efficiency.
Ursodeoxycholic acid enriches intestinal bile salt hydrolase-expressing Bacteroidetes in cholestatic 1 pregnancy 2

C4 negative on de novo hepatic bile acid synthesis through fibroblast growth factor receptor 4/ beta-klotho hepatic signalling. of bile in This study supports the conclusion that the secondary modifications of UDCA by bacteria to metabolically-active bile acids are important in delivering the intestinally-derived benefits of UDCA treatment in ICP. Although unconjugated UDCA is delivered to the intestines from the ingested medication for treated women, the efficiency of the enterohepatic circulation and subsequent conjugation in the liver enable subsequent biliary secretion of conjugated UDCA. Modification of this to LCA or CDCA requires cleavage from the conjugated bile acid by bacterial bile salt hydrolase. Hence, we conclude that an intestinal environment favourable to bile salt hydrolase-producing bacteria is likely to enhance enterohepatic feedback. Women with a high ratio of Bacteroidetes : Firmicutes were more likely to be taking UDCA (7/8), however others taking UDCA had a low ratio (6/14), and correspondingly reduced bile salt hydrolase activity in the faeces. This biological variation may explain the differing clinical responses to UDCA that we have observed for women with ICP[34], and provide an additional treatment target (enriching the intestinal microbiota with bile salt hydrolase-producing bacteria) to provide in combination with UDCA for the treatment 236 of ICP. Identification of women with a high Bacteroidetes : Firmicutes signature prior to treatment may predict better response to treatment with UDCA, and future studies could use this as a predictive tool to treatment response. Alternatively, the observation that women with a high ratio of Bacteroidetes : Firmicutes had taken significantly more UDCA prior to sample donation than those 240 women with a lower ratio suggests that there is a dose-response to UDCA treatment that may affect the composition of the gut microbiota. The lower quartile of UDCA exposure for women with high Bacteroidetes : Firmicutes was more than 2g/day for one week, providing the first evidence for minimal effective dosing for UDCA in ICP.
mir-517a promotes Warburg effect in hcc by directly targeting FBP1

Purpose: Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies world-wide. Our aim is to explore the expression and biological function of miR-517a in HCC. Materials and methods: We performed qRT-PCR to detect the expression of miR-517a in clinical samples and cell lines. CKK-8 assay and colony formation assay were employed to detect the miR-517a regulated cell proliferation. Glucose uptake and lactate production were examined to determine the Warburg effect. We also performed ECAR assay using Seahorse system. Luciferase acitivy assay was used to examine the binding of FBP1 3’UTR by miR-517a. Results: miR-517a was upregulated in HCC samples in both genomic and mRNA levels. More-over, overexpression of miR-517a promoted cell proliferation and Warburg effect. Mechanically, miR-517a could directly target the 3 ′ -UTR of FBP1. In addition, restoring the expression of FBP1 inhibited cell growth. Conclusion: We demonstrated that miR-517a acts as an oncogene to promote Warburg effect in HCC, favoring tumor growth, and miR-517a/FBP1 could be a novel target for HCC treatment.
Vicarious Trauma: An Exploratory Study of the Impact of Providing Sexual Abuse Treatment on Clinicians' Trust and Intimacy

ABSTRACT This study examined vicarious trauma effects in male and female clinicians who treat sexual abuse survivors (n = 111) and sexual offenders (n = 272). The national survey was conducted using a random sample of clinical members of two professional organizations. Analyses tested the relationships between demographic variables, maltreatment history, client population served, and cognitions about trust of and intimacy with others, using the Trauma Stress Institute Belief Scale (TSIBS-R-L, Pearlman 2003), the Childhood Trauma Questionnaire (CTQ, Bernstein & Fink, 1998), and author-generated questions. Respondents reported high rates of multiple forms of childhood maltreatment; however there was no relationship between history of child sexual abuse and vicarious trauma effects. Scores for self-reported disruption in cognitions about intimacy with others exceeded norms for mental health professionals. Sequential regression analyses were used to examine theoretically-derived variables. Implications for practice and research are detailed.
Fish loss to irrigation canals and methods to reduce these losses on the West Gallatin River, Montana

Agriculture and recreational fisheries are important to Montana’s economy, however they compete for the state’s water resources. Conflicts over the number of trout entering irrigation canals, and subsequently being lost from a river’s fishery, prompted concern, in Montana, as early as 1893. Yet few studies have examined the effects of fish loss to irrigation diversions on trout populations. I estimated the number of trout (> 150mm) in gravity-fed irrigation canals on the West Gallatin River, Montana, by trapping and electrofishing, in 1993 and 1994. It was presumed that fish entering the canals were lost from the West Gallatin River fishery. Trout abundance was related to habitat types and canal characteristics and operations. Multiple regression was used to assess the relationship between these factors and population abundance. Trout population densities varied from 0-16 fish/lOOm (median 1.5 fish/100m). Total trout populations ranged between 0 and 1,905 fish/canal (median 215 fish). Repeated electrofishing of identical transects found no significant differences between trout populations in 1993 and 1994 (P-values >0.26, Betas >0.92) or between June 1994 and September 1994 (P-values >0.12, Betas >0.83). Most trout entered the irrigation canals during periods of maximum river flow. I measured the following canal characteristics: headgate size, manipulation, construction materials and habitat; intake size, habitat and angle in relation to the river’s thalweg; and flow; which were used to develop a predictive model of the number of trout found in a canal. The model contained headgate area, intake angle in relation to the river’s thalweg and intake length [canal populations = -2,429.7 + 0.7246(earea) + 0.5405(l/50m angle) 0.3287(sqrt(intake length))] (model R2= 0.87, P-value = 0.00483). Large canals, with short intake channels, located on the outside of river bends captured more fish than canals with opposite characteristics. Ajackknife bias-estimation procedure indicated that the model overfit the data (R2 = 0.25, P-value > 0.80). The model predicted canal trout populations to be four times greater than the West Gallatin River’s study area trout population (140,353 and 34,132, respectively). Two canals contained 87% of the model’s predicted trout abundances, and the headgate area variables for both canals were above the range of data collected in the study canals. Rapid (90%) and staged (30%) drawdown regimes were evaluated to encourage trout movement. Among the regimes examined, reducing canal flow by 90% while leaving adequate flows for fish <movement was most effective in stimulating up-canal movement. Therefore, altering V the time of canal openings to avoid a river’s period peak discharge and reducing canal flows by 90%, with leaving the remaining flow at least 2 d before completely closing / the headgate, might decrease the numbers of fish stranded in canals at the end of an / irrigation season. z
Recent development in the strategies projected for chikungunya vaccine in humans

: The unprecedented epidemic spread of chikungunya worldwide illustrates the critical need for potent vaccines and therapeutic interventions. The morbidity and mortality associated with this arboviral infection has become a major public health problem in many countries across different continents. Increasing public–private partnerships have opened new avenues in research and development of vaccines. This review mainly focuses on the recent advances in patented approaches for chikungunya vaccine development and the forthcoming challenges.
Food Microbiology: A Laboratory Manual

PREFACE.PART I. BASICS OF FOOD MICROBIOLOGY LABORATORY.1. BASIC MICROBIOLOGICAL TECHNIQUES.PART II. FOOD MICROBIOTA.2. TOTAL PLATE COUNT.3. YEASTS AND MOLDS.4. COLIFORM COUNT IN FOOD.5. MESOPHILIC AEROBIC AND ANAEROBIC SPORES.6. MICROBIOTA OF FOOD PROCESSING ENVIRONMENT.PART III. FOOD-TRANSMITTED PATHOGENS.7. Staphylococcus aureus.8. Listeria monocytogenes.9. Salmonella.10. Escherichia coli O157:H7.PART IV. FOOD FERMENTATION.11. LACTIC ACID FERMENTATION AND BACTERIOCIN PRODUCTION.APPENDIX A: LABORATORY EXERCISE REPORT.APPENDIX B: MICROBIAL GROWTH KINETICS.APPENDIX C: MICROBIOLOGICAL MEDIA.INDEX.
Biomarker-based detection of asthma–COPD overlap syndrome in COPD populations

: Asthma–chronic obstructive pulmonary disease (COPD) overlap syndrome (ACOS) was proposed by the science committees of both Global Initiative for Asthma (GINA) and Global Initiative for Chronic Obstructive Lung Disease (GOLD). However, the definition of ACOS has remained unclear all over the world, and the prevalence rate of ACOS is basically dependent on the patient’s symptoms or the physician’s opinion, based on questionnaire testing. In the current case report, we investigated the prevalence rate of COPD patients with high levels of fractional exhaled nitric oxide (FENO) or immunoglobulin E (IgE) as candidate markers of ACOS in COPD, as a multicenter, cross-sectional study. Outpatients with COPD were enrolled from Tohoku University Hospital, Sendai, Japan, and five hospitals (Tohoku University Hospital, Sendai, Japan; NTT East Tohoku Hospital, Sendai, Japan; Wakayama Medical University Hospital, Kimiidera, Japan; Hiraka General Hospital, Yokote, Japan; Iwate Prefectural Isawa Hospital, Oshu, Japan) with pulmonary physicians from March 1, 2013 to February 28, 2014. When they were estimated using 35 ppb as the cutoff value of FENO, the prevalence rate of ACOS was 16.3% in COPD. When estimated by both FENO and IgE, the high-FENO/high-IgE group was 7.8% in COPD. To the best of our knowledge, this study is the first to detect the prevalence rate of ACOS in COPD populations by using objective biomarkers. The results from the current study should be useful to identify the subgroup requiring early intervention by inhaled corticosteroids/long-acting beta agonist combination in COPD in order to improve the long-term management for ACOS.
The role of biotic factors during plant establishment in novel communities

7 Background. Establishment success of non-native species can be influenced by interactions with 8 native competitors and novel enemies. Magnitude of interactions between species is mediated by 9 traits, e.g. reflecting competitive strength or defence mechanisms. We combine the concepts to 10 investigate the importance of species traits for successful establishment in a novel community 11 exhibiting biotic resistance in the form of competition and herbivory. 12
PRMT7 contributes to the metastasis phenotype in human non-small-cell lung cancer cells possibly through the interaction with HSPA5 and EEF2

Background: Non-small-cell lung cancer (NSCLC) constitutes the leading cause of cancer death in humans. Previous studies revealed the essential role of the protein arginine methyltransferase 7 ( PRMT7 ) in promoting metastasis in breast cancer. However, its function and potential mechanism in NSCLC remain unclear. Materials and methods: The gene expression of PRMT7 between lung cancer tissues and normal tissues was studied with online database (http://medicalgenome.kribb.re.kr/ GENT/). NSCLC cell lines with specific gene overexpression were constructed with lentivirus transduction. Matrigel invasion and colony formation assays were performed to evaluate the invasion and colony formation abilities. Co-immunoprecipitation coupled with mass spectrometry analysis was performed to explore the potential interaction proteins of PRMT7 . Bioinformatic analysis was performed with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Results: Online analysis of gene expression patterns revealed the relatively high expression of PRMT7 in lung cancer tissues. PRMT7 overexpression was able to promote the invasion and colony formation of A549 and SPC-A1 cells. A total of 19 in-common proteins shared by both NSCLC cell lines were identified to be interacting with PRMT7 and found to participate in a wide variety of pathways and protein–protein interactions according to bioinformatic analysis. Among them, HSPA5 and EEF2 were further investigated for their essential roles in PRMT7 promoted NSCLC cell invasion. Conclusion: Our results suggested PRMT7 overexpression was able to promote metastasis in NSCLC possibly through the interaction with HSPA5 and EEF2 , which provides the potential mechanism of oncogenesis in lung cancer.
Three-dimensional scene recovery for measuring sighting distances of rail track assets from monocular forward facing videos

Rail track asset sighting distance must be checked regularly to ensure the continued and safe operation of rolling stock. Methods currently used to check asset line-of-sight involve manual labour or laser systems. Video cameras and computer vision techniques provide one possible route for cheaper, automated systems. Three categories of computer vision method are identified for possible application: two-dimensional object recognition, two-dimensional object tracking and three-dimensional scene recovery. However, presented experimentation shows recognition and tracking methods produce less accurate asset line-of-sight results for increasing asset-camera distance. Regarding three-dimensional scene recovery, evidence is presented suggesting a relationship between image feature and recovered scene information. A novel framework which learns these relationships is proposed. Learnt relationships from recovered image features probabilistically limit the search space of future features, improving efficiency. This framework is applied to several scene recovery methods and is shown (on average) to decrease computation by two-thirds for a possible, small decrease in accuracy of recovered scenes. Asset line-of-sight results computed from recovered three-dimensional terrain data are shown to be more accurate than two-dimensional methods, not effected by increasing asset-camera distance. Finally, the analysis of terrain in terms of effect on asset line-of-sight is considered. Terrain elements, segmented using semantic information, are ranked with a metric combining a minimum line-of-sight blocking distance and the growth required to achieve this minimum distance. Since this ranking measure is relative, it is shown how an approximation of the terrain data can be applied, decreasing computation time. Further efficiency increases are found by decomposing the problem into a set of two-dimensional problems and applying binary search techniques. The combination of the research elements presented in this thesis provide efficient methods for automatically analysing asset line-of-sight and the impact of the surrounding terrain, from captured monocular video.
Local residents' attitudes toward potential tourism development: the case of Ansted, West Virginia

The purpose of this study was to understand residents' attitudes toward tourism development in the town of Ansted, WV, using self-administered surveys. The attitude assessment in this study was part of a tourism planning process conducted for the town. The results indicate that perceptions of tourism development among Ansted's residents are generally homogeneous and highly positive. They do not believe that issues normally resulting from increased tourism, such as crowding, increased prices, or pollution would be a problem for the community. Rather, they are inclined to support tourism development for its potential to spur local economic development and provide related benefits. This finding is consistent with the social exchange theory that the more dependent a community is on economic benefits, the more likely it is that the community will support tourism development. The findings of this research will be an important contribution to a plan for sustainable tourism development in Ansted.
CRISPR-C: circularization of genes and chromosome by CRISPR in human cells

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dualfluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. We show that CRISPR-C can generate functional eccDNA, using the novel eccDNA biosensor system. We further reveal that CRISPR-C also can generate eccDNAs from intergenic and genic loci in human embryonic kidney 293T cells and human mammary fibroblasts. EccDNAs mainly forms by end-joining mediated DNA-repair and we show that CRISPR-C is able to generate endogenous eccDNAs in sizes from a few hundred Article Navigation    We use cookies to enhance your experience on our website. By clicking 'continue' or by continuing to use our website, you are agreeing to our use of cookies. You can change your cookie settings at any time. Continue Find out more 1/32 CRISPR-C: circularization of genes and chromosome by CRISPR in human cells | Nuc. 2019/2/28 https://academic.oup. com/nar/article/46/22/e131/5078801 Subject: Targeted gene modification, Genomics Issue Section: Methods Online
Methodology for Recreation Data Acquisition and Evaluation for Ethnic Minority Visitors to Corps of Engineers Projects

Abstract : A survey was conducted at five Corps of Engineers (CE) lakes during the spring and summer of 1999 to obtain baseline information about the outdoor recreation style and preferences of these ethnic minority groups (African, Hispanic, and Asian Americans) and their experiences and perceptions at CE projects. This report documents the results of that survey. The study also tested the draft survey instrument against results obtained in four focus group interviews which were conducted in 1998 at the same CE lakes. The 1999 survey population included African Americans at Woodruff Lake, AL, and Carlyle Lake, IL; Hispanic Americans at Canyon Lake, TX, and Pine Flat Lake, CA; and Asian Americans at Hensley Lake, CA, and Pine Flat Lake, CA. The survey was conducted from May 15 to July 30, 1999. While Native Americans were not included in this survey, the report contains a discussion of six focus groups which were conducted during the summer of 1997 with fifteen Native American tribes located in the CE's Tulsa and Omaha Districts.
mir-106b-5p promotes stem cell-like properties of hepatocellular carcinoma cells by targeting PTen via Pi3K/akt pathway in cancer heterogeneity, metastasis and drug resistance. 5 With recent

Background: The miRNA miR-106b-5p has been previously reported to be increased in hepatocellular carcinoma (HCC) tissues compared to cirrhotic tissues. The purpose of this study was to detect its expression in HCC cell lines with distinct metastatic potentials and to explore the molecular mechanisms underlying HCC stemness and migration. Methods: miR-106b-5p expression was studied in HCC tissues and cell lines. In vitro cancer stem cell (CSC)-like properties, cell migration and invasion were compared between HCC cell lines with upregulation or downregulation of miR-106b-5p. In vivo tail vein injection models were established to evaluate the role of miR-106b-5p in lung metastasis. Bioinformatics programs, luciferase reporter assay and rescue experiments were used to validate the downstream targets of miR-106b-5p. The relationship between the expression of the targeted gene and clinicopathological parameters was also analyzed. Results: miR-106b-5p expression was higher in HCC tissues and cell lines than that in non-tumor tissues and hepatocyte Chang liver, respectively. Upregulation of miR-106b-5p exhibited a promoting role in CSC properties, cell migration and activation of phosphatidylinositol-3 kinase (PI3K)/Akt signaling in vitro, as well as in lung metastasis in vivo. However, downregulation of miR-106b-5p exhibited the opposite effect. Furthermore, PTEN was verified as a direct target of miR-106b-5p. Upon clinicopathological analysis, lower level of PTEN was significantly associated with more aggressive characteristics. Patients with high PTEN expression had longer overall survival and disease-free survival. Conclusion: miR-106b-5p promotes HCC stemness maintenance and metastasis by targeting PTEN via PI3K/Akt pathway. Inhibition of miR-106b-5p might be effective therapeutic strate-gies to treat advanced HCC. as a control and were normalized to the level of chang liver cells. Abbreviations: csc, cancer stem cell; PTen, phosphatase and tensin homolog; nc, negative control.