Miracle-dz/UniFAR-FTUs huggingface dataset:
Partitioning genetic effects on birthweight at classical human leukocyte antigen loci into maternal and fetal components, using structural equation modelling

Abstract Background Single nucleotide polymorphisms in the human leukocyte antigen (HLA) region in both maternal and fetal genomes have been robustly associated with birthweight (BW) in previous genetic association studies. However, no study to date has partitioned the association between BW and classical HLA alleles into maternal and fetal components. Methods We used structural equation modelling (SEM) to estimate the maternal and fetal effects of classical HLA alleles on BW. Our SEM leverages the data structure of the UK Biobank (UKB), which includes ∼270 000 participants’ own BW and/or the BW of their firstborn child. Results We show via simulation that our model yields asymptotically unbiased estimates of the maternal and fetal allelic effects on BW and appropriate type I error rates, in contrast to simple regression models. Asymptotic power calculations show that we have sufficient power to detect moderate-sized maternal or fetal allelic effects of common HLA alleles on BW in the UKB. Applying our SEM to imputed classical HLA alleles and own and offspring BW from the UKB replicated the previously reported association at the HLA-C locus and revealed strong evidence for maternal (HLA-A*03:01, B*35:01, B*39:06, P <0.001) and fetal allelic effects (HLA-B*39:06, P <0.001) of non-HLA-C alleles on BW. Conclusions Our model yields asymptotically unbiased estimates, appropriate type I error rates and appreciable power to estimate maternal and fetal effects on BW. These novel allelic associations between BW and classical HLA alleles provide insight into the immunogenetics of fetal growth in utero.
Work‐related consequences of losing a child with cancer: A nationwide population‐based cohort study

Parents who lose a child are at increased risk of impaired mental health, which may negatively affect their work ability. The aims of this study were to examine the risk for reduced labor market affiliation in parents who lost a child with cancer compared to a matched parent cohort, and factors associated with the bereaved parents’ labor market affiliation.
Running, jumping, hunting, and scavenging: Functional analysis of vertebral mobility and backbone properties in carnivorans

Carnivorans are well‐known for their exceptional backbone mobility, which enables them to excel in fast running and long jumping, leading to them being among the most successful predators amongst terrestrial mammals. This study presents the first large‐scale analysis of mobility throughout the presacral region of the vertebral column in carnivorans. The study covers representatives of 6 families, 24 genera and 34 species. We utilized a previously developed osteometry‐based method to calculate available range of motion, quantifying all three directions of intervertebral mobility: sagittal bending (SB), lateral bending (LB), and axial rotation (AR). We observed a strong phylogenetic signal in the structural basis of the vertebral column (vertebral and joint formulae, length proportions of the backbone modules) and an insignificant phylogenetic signal in most characteristics of intervertebral mobility. This indicates that within the existing structure (stabilization of which occurred rather early in different phylogenetic lineages), intervertebral mobility in carnivorans is quite flexible. Our findings reveal that hyenas and canids, which use their jaws to seize prey, are characterized by a noticeably elongated cervical region and significantly higher SB and LB mobility of the cervical joints compared to other carnivorans. In representatives of other carnivoran families, the cervical region is very short, but the flexibility of the neck (both SB and LB) is significantly higher than that of short‐necked odd‐toed and even‐toed ungulates. The lumbar region of the backbone in carnivorans is dorsomobile in the sagittal plane, being on average ~23° more mobile than in artiodactyls and ~38° more mobile than in perissodactyls. However, despite the general dorsomobility, only some representatives of Canidae, Felidae, and Viverridae are superior in lumbar flexibility to the most dorsomobile ungulates. The most dorsomobile artiodactyls are equal or even superior to carnivorans in their ability to engage in dorsal extension during galloping. In contrast, carnivorans are far superior to ungulates in their ability to engage ventral flexion. The cumulative SB in the lumbar region in carnivorans largely depends on the mode of running and hunting. Thus, adaptation to prolonged and enduring pursuit of prey in hyenas is accompanied by markedly reduced SB flexibility in the lumbar region. A more dorsostable run is also a characteristic of the Ursidae, and the peculiar maned wolf. Representatives of Felidae and Canidae have significantly more available SB mobility in the lumbar region. However, they fully engage it only occasionally at key moments of the hunt associated with the direct capture of the prey or when running in a straight line at maximum speed.
Plasticity in Preganglionic and Postganglionic Neurons of the Sympathetic Nervous System during Embryonic Development

Abstract Sympathetic preganglionic neurons (SPNs) are the final output neurons from the central arm of the autonomic nervous system. Therefore, SPNs represent a crucial component of the sympathetic nervous system for integrating several inputs before driving the postganglionic neurons (PGNs) in the periphery to control end organ function. The mechanisms which establish and regulate baseline sympathetic tone and overall excitability of SPNs and PGNs are poorly understood. The SPNs are also known as the autonomic motoneurons (MNs) as they arise from the same progenitor line as somatic MNs that innervate skeletal muscles. Previously our group has identified a rich repertoire of homeostatic plasticity (HP) mechanisms in somatic MNs of the embryonic chick following in vivo synaptic blockade. Here, using the same model system, we examined whether SPNs exhibit similar homeostatic capabilities to that of somatic MNs. Indeed, we found that after 2-d reduction of excitatory synaptic input, SPNs showed a significant increase in intracellular chloride levels, the mechanism underlying GABAergic synaptic scaling in this system. This form of HP could therefore play a role in the early establishment of a setpoint of excitability in this part of the sympathetic nervous system. Next, we asked whether homeostatic mechanisms are expressed in the synaptic targets of SPNs, the PGNs. In this case we blocked synaptic input to PGNs in vivo (48-h treatment), or acutely ex vivo, however neither treatment induced homeostatic adjustments in PGN excitability. We discuss differences in the homeostatic capacity between the central and peripheral component of the sympathetic nervous system.
The Impact of Sediment Consistency on Track and Undertrack Morphology: Experiments with Emu Tracks in Layered Cement

To demonstrate the influence of sediment consistency on track and undertrack morphology, an emu foot was impressed in four packages of layered, colored cement. The packages contained different ratios of cement to water and were subsequently sliced vertically to reveal the subsurface deformation caused by the foot, and to expose the differences in track and undertrack morphology at different degrees of firmness. A further package of alternating layers of sand and cement layers was prepared, allowing the package to be spilt along horizontal surfaces in order to investigate the undertrack formation and loss of information with depth. The experiments clearly show that increasing water content in the sediment has a strong effect on the morphology of both the true track and the undertracks.
Network pharmacology-based strategy for predicting therapy targets of Ecliptae Herba on breast cancer

Breast cancer is a prevalent malignancy affecting women globally, characterized by significant morbidity and mortality rates. Ecliptae Herba is a traditional herbal medicine commonly used in clinical practice, has recently been found to possess antitumor properties. In order to explore the underlying material basis and molecular mechanisms responsible for the anti-breast cancer effects of Ecliptae Herba, we used network pharmacology and experimental verification. UPLC-MS/MS was utilized to identify compounds present in Ecliptae Herba. The active components of Ecliptae Herba and its breast cancer targets were screened using public databases. Hub genes were identified using the STRING and Metascape database. The R software was utilized for visual analysis of GO and KEGG pathways. The affinity of the hub targets for the active ingredients was assessed by molecular docking analysis, which was verified by experimental assessment. A total of 178 targets were obtained from the 10 active components of Ecliptae Herba, while 3431 targets associated with breast cancer were screened. There were 144 intersecting targets between the components and the disease. Targets with a higher degree, namely EGFR and TGFB1, were identified through the hub subnetwork of PPI. GO and KEGG analyses revealed that Ecliptae Herba plays an important role in multiple cancer therapeutic mechanisms. Moreover, molecular docking results showed that the core components had good binding affinity with key targets. Finally, it was confirmed that TGF-β1 might be a potential crucial target of Ecliptae Herba in the treatment of breast cancer by cytological experiments, and the TGF-β1/Smad signaling pathway might be an important pathway for Ecliptae Herba to exert its therapeutic effects. This study elucidated the active ingredients, key targets, and molecular mechanisms of Ecliptae Herba in the treatment of breast cancer, providing a scientific foundation and therapeutic mechanism for the prevention and treatment of breast cancer with Traditional Chinese medicine.
Successful chelation in beta-thalassemia major in the 21st century

This century has seen a revolution the management of beta-thalassemia major. Over a 12-year period to 2016, we aimed to analyze the benefits of such advances. In 209 patients, independent of the chelation regimen, ferritin, cardiac T2* and liver iron concentration changes were evaluated. We defined chelation success (ChS) as no iron load in the heart and acceptable levels in the liver. Over 3 early magnetic resonance imagings, the same parameters were assessed in 2 subgroups, the only 2 that had sufficient patients continuing on 1 regimen and for a significant period of time, 1 on deferrioxamine (low iron load patients n = 41, Group A) and 1 on deferoxamine-deferiprone (iron overloaded n = 60, Group B). Finally, 28 deaths and causes were compared to those of an earlier period. The 209 patients significantly optimized those indices, while the number of patients with chelation success, increased from 6% to 51% (P < .0001). In group A, ChS after about 8 years increased from 21 to 46% (P = .006), while in Group B, from 0% to 60% (P < .001) after about 7 years. Deaths over the 2 periods showed significant reduction. Combined clearance of cardiac and liver iron (ChS) is feasible and should become the new target for all patients. This requires, serial magnetic resonance imagings and often prolonged intensified chelation for patients.
Cloud and radiative characteristics of tropical deep convective systems in extended cloud objects from CERES observations.

Abstract The physical and radiative properties of tropical deep convective systems for the period from January to August 1998 are examined with the use of Clouds and the Earth’s Radiant Energy System Single-Scanner Footprint (SSF) data from the Tropical Rainfall Measuring Mission satellite. Deep convective (DC) cloud objects are contiguous regions of satellite footprints that fulfill the DC criteria (i.e., overcast footprints with cloud optical depths >10 and cloud-top heights >10 km). Extended cloud objects (ECOs) start with the original cloud object but include all other cloudy footprints within a rectangular box that completely covers the original cloud object. Most of the non-DC footprints are overcast but have optical depths and/or cloud-top heights that are too low to fit the DC criteria. The histograms of cloud physical and radiative properties are analyzed according to the size of the ECO and the SST of the underlying ocean. Larger ECOs are associated with greater magnitudes of large-scale upward .
Risk factors of disturbed sleep phases to posterior circulation cerebral infarctions: A single-center retrospective study

Posterior circulation stroke differs from anterior circulation stroke in terms of etiological, clinical, and prognostic properties. Sleep architecture is impaired in patients with acute stroke, which may correlate with disease severity and outcome, and the correlation between the location of cerebral infarction (CI) and sleep phase disturbance remains unknown. This study aimed to assess the correlation between disturbed sleep phases in CI and posterior circulation cerebral infarction (PCCI). We retrospectively enrolled 192 patients with first-onset acute CI, who were assigned to the anterior circulation cerebral infarction (n = 101) and PCCI (n = 91) groups. The polysomnograms in both groups were analyzed by phase. The proportions of sleep phases were significantly different between the 2 groups (P < .05). The awake (W) and non-rapid eye movement 3 (N3) phases were independently associated with PCCI in multivariate analysis. The W phase may be a risk factor for PCCI (odds ratio = 1.60, 95% CI 1.30–1.97), while the N3 phase may be a protective factor for PCCI (odds ratio = 0.498, 95% CI 0.353–0.703). This study demonstrated that CI causes different degrees of sleep phase disturbances, and the percentages of W and N3 phase disturbances were independent factors associated with PCCI. The former was a risk factor, whereas the latter was a protective factor. This study demonstrated the correlation between cerebral infarction and sleep phase disturbances from a new perspective and suggested that cerebral infarcts may alter the structure of sleep.
Calcium and magnesium‐limited dolomite precipitation at Deep Springs Lake, California

Dolomite [Ca,Mg(CO3)2] precipitation from supersaturated ionic solutions at Earth surface temperatures is considered kinetically inhibited because of the difficulties experienced in experimentally reproducing such a process. Nevertheless, recent dolomite is observed to form in hypersaline and alkaline environments. Such recent dolomite precipitation is commonly attributed to microbial mediation because dolomite has been demonstrated to form in vitro in microbial cultures. The mechanism of microbially mediated dolomite precipitation is, however, poorly understood and it remains unclear what role microbial mediation plays in natural environments. In the study presented here, simple geochemical methods were used to assess the limitations and controls of dolomite formation in Deep Springs Lake, a highly alkaline playa lake in eastern California showing ongoing dolomite authigenesis. The sediments of Deep Springs Lake consist of unlithified, clay‐fraction dolomite ooze. Based on δ18O equilibria and textural observations, dolomite precipitates from oxygenated and agitated surface brine. The Na‐SO4‐dominated brine contains up to 500 mm dissolved inorganic carbon whereas Mg2+ and Ca2+ concentrations are ca 1 and 0·3 mm, respectively. Precipitation in the subsurface probably is not significant because of the lack of Ca2+ (below 0·01 mm). Under such highly alkaline conditions, the effect of microbial metabolism on supersaturation by pH and alkalinity increase is negligible. A putative microbial effect could, however, support dolomite nucleation or support crystal growth by overcoming a kinetic barrier. An essential limitation on crystal growth rates imposed by the low Ca2+ and Mg2+ concentrations could favour the thermodynamically more stable carbonate phase (which is dolomite) to precipitate. This mode of unlithified dolomite ooze formation showing δ13C values near to equilibrium with atmospheric CO2 (ca 3‰) contrasts the formation of isotopically light (organically derived), hard‐lithified dolomite layers in the subsurface of some less alkaline environments. Inferred physicochemical controls on dolomite formation under highly alkaline conditions observed in Deep Springs Lake may shed light on conditions that favoured extensive dolomite formation in alkaline Precambrian oceans, as opposed to modern oceans where dolomites only form diagenetically in organic C‐rich sediments.
Environmental and social impact assessment

PART I: MACRO IMPACT ASSESSMENT: Environmental Impact Assessment Social Impact Assessment Technology Assessment: The State of the Art Policy Assessment PART II: TOOLS OF IMPACT ASSESSMENT: Economic and Fiscal Impact Assessment Demographic Impact Assessment Health Impact Assessment of Development Projects Ecological Impact Assessment Risk Assessment Public Involvement: From Consultation to Participation PART III: TOOLS FOR THE FUTURE: Climate Impact Assessment Development Impact Assessment: Impact Assessment of Aid Projects in Non-Western Countries Environmental Auditing Environmental Sustainability.
Representativeness of arctic weather station data for the computation of snowmelt in a small area

This study determines how representative the snowmelt values computed using arctic weather station data are of the melt in its surrounding area. Simultaneous measurements of meteorological variables were made at several sites to permit comparisons of their calculated snowmelt with the weather station at Resolute. Northwest Territories, Canada. Like most other stations, the Resolute site is located near the coast, at an airport and close to human settlement, making it warmer and its snow albedo lower than its adjacent sites. Snowmelt rates at Resolute are higher than that of a flat site away from the airport, This latter site has snowmelt conditions more typical of the rolling terrain nearby, but its melt rates are higher than those for an inland site where the snow remains longer than at the coastal zone, Through these simultaneous observations and systematic comparisons, this study indicates that the point data from coastal, arctic stations are unlikely to be representative of their surrounding areas. Thus, caution should be exercised when applying such information directly to the computation of snowmelt for entire grid cells of macro-hydrologic models.
Coupled vegetation‐precipitation variability observed from satellite and climate records

Variability in precipitation regimes at seasonal and longer time scales strongly influences ecosystem dynamics in arid and semi‐arid regions. In this paper, we use time series of global precipitation and satellite normalized difference vegetation index (NDVI) data to analyze joint spatial and temporal variability between terrestrial ecosystems and precipitation regimes. Accumulated monthly rainfall anomalies are quantified using a standardized precipitation index (SPI), which provides a better measure of ecologically significant precipitation excess or deficit at growing season time scales relative to monthly precipitation data. Results from canonical correlation analysis reveal geographically extensive patterns of joint NDVI‐SPI variability suggestive of strong climate‐biosphere coupling. Further, leading modes of covariability are shown to be related to large‐scale ocean‐atmosphere circulation anomalies. These results illustrate the global extent and sensitivity of ecosystems susceptible to climate change‐induced perturbations in precipitation regimes.
Effects of Land Use Change on Hydrologic Response at a Watershed Scale, Arkansas

AbstractThe population has increased at a fast pace in the last two decades in Arkansas. One direct result from this process is urbanization, especially in northwest Arkansas. Land use and land cover (LULC) had been changed from forests, pastures, and farmland to urbanized areas over a short time span. Increased flood discharge and decreased water quality have been negative side effects of urbanization on the environment. In this study, five watersheds were selected as representative of the entire state. These watersheds were selected to examine the hydrological response to land use change over the last two decades in Arkansas. The curve number, a term used to describe the runoff depth, was calculated for the five watersheds. Out of the five watersheds, the Illinois River watershed experienced the greatest change in land use from 1993–2006, whereas the other four watersheds experienced slight land use change. The land transformation model (LTM) was used to forecast the land use of the Illinois River water.
Can EPOS2020 criteria of type 2 inflammation be applied to Asian patients with chronic rhinosinusitis?

Abstract Background There is a large diversity of mucosal immunologic chronic rhinosinusitis with nasal polyps (CRSwNP) endotypes across Western and Asian patient populations. Objectives The objective of the study was whether the European Position Paper on Rhinosinusitis and Nasal Polyps (EPOS) 2020 criteria for type 2 inflammation are appropriate for biological use in CRSwNP patients. Methods A total of 207 participants are enrolled for the study. Retrospective evaluations of the tissues taken during surgery and the patients’ clinical features were performed. We investigated whether the criteria described in the EPOS2020 guideline were appropriate based on the criteria for type 2 inflammation identified based on prior studies using receiver-operating characteristic (ROC) analyses. Results The EPOS 2020 criteria are also shown to be an insufficient evaluation approach with low specificity (area under curve [AUC] = 0.645, specificity 8.4%). The authors created a novel scoring method using the total serum IgE level, blood eosinophil percentage, and tissue eosinophil percentage. This novel scoring system (AUC = 0.862, p < .001) fared better in ROC analyses than the EPOS 2020 criteria (AUC = 0.645) and Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis scoring system (AUC = 0.647). Conclusions and Significance A novel standard for type 2 inflammation in Asian CRSwNP patients must be established, as the EPOS 2020 criteria do not appear to be sufficient.
Optimal ship forms for minimum total resistance in shallow water

I NOMENCLATURE INTRODUCTION 3 FORMULATION OF THE PROBLEM 5 Expressions for Shallow-water Wave-Making Resistance Expressions for Shallow-water Viscous Resistance 5 15 THE RESULTS OF CALCULATION 18 Calculation of Shallow-water Resistance and Comparison with Experimental Results 18 The Results of Optimization for Wave Resistance 21 The Results of Optimization for Total Resistance 29 CLOSING REMARKS 56 ACKNOWLEDGEMENT 58 REFERENCES 59
Genomic Heterogeneity of Cryptosporidium parvum Isolates From Children in Bangladesh: Implications for Parasite Biology and Human Infection

Abstract Cryptosporidium species are a major cause of diarrhea and associated with growth failure. There is currently only limited knowledge of the parasite's genomic variability. We report a genomic analysis of Cryptosporidium parvum isolated from Bangladeshi infants and reanalysis of sequences from the United Kingdom. Human isolates from both locations shared 154 variants not present in the cattle-derived reference genome, suggesting host-specific adaptation of the parasite. Remarkably 34.6% of single-nucleotide polymorphisms unique to human isolates were nonsynonymous and 8.2% of these were in secreted proteins. Linkage disequilibrium decay indicated frequent recombination. The genetic diversity of C. parvum has potential implications for vaccine and therapeutic design. Clinical Trials Registration. NCT02764918.