Miracle-dz/UniFAR-FTUs huggingface dataset:
Capturing Spatial Influence in Wind Prediction With a Graph Convolutional Neural Network

Nowadays, wind power is playing a significant role in power systems; it is necessary to improve the prediction accuracy, which will help make better use of wind sources. The existing neural network methods, such as recurrent neural network (RNN), have been widely used in wind prediction; however, RNN models only consider the dynamic change of temporal conditions and ignore the spatial correlation. In this work, we combine the graph convolutional neural (GCN) with the gated recurrent unit (GRU) to do prediction on simulated and real wind speed and wind power data sets. The improvements of prediction results by GCN in all wind speed experiments show its ability to capture spatial dependence and improve prediction accuracy. Although the GCN does not perform well in short-term wind power prediction as the change of wind power data is not so smooth due to the limitation of turbine operation, the results of long-term prediction still prove the performance of GCN.
Knee Joint Menisci Are Shock Absorbers: A Biomechanical In-Vitro Study on Porcine Stifle Joints

The aim of this biomechanical in vitro study was to answer the question whether the meniscus acts as a shock absorber in the knee joint or not. The soft tissue of fourteen porcine knee joints was removed, leaving the capsuloligamentous structures intact. The joints were mounted in 45° neutral knee flexion in a previously validated droptower setup. Six joints were exposed to an impact load of 3.54 J, and the resultant loss factor (η) was calculated. Then, the setup was modified to allow sinusoidal loading under dynamic mechanical analysis (DMA) conditions. The remaining eight knee joints were exposed to 10 frequencies ranging from 0.1 to 5 Hz at a static load of 1210 N and a superimposed sinusoidal load of 910 N (2.12 times body weight). Forces (F) and deformation (l) were continuously recorded, and the loss factor (tan δ) was calculated. For both experiments, four meniscus states (intact, medial posterior root avulsion, medial meniscectomy, and total lateral and medial meniscectomy) were investigated. During the droptower experiments, the intact state indicated a loss factor of η = 0.1. Except for the root avulsion state (−15%, p = 0.12), the loss factor decreased (p < 0.046) up to 68% for the total meniscectomy state (p = 0.028) when compared to the intact state. Sinusoidal DMA testing revealed that knees with an intact meniscus had the highest loss factors, ranging from 0.10 to 0.15. Any surgical manipulation lowered the damping ability: Medial meniscectomy resulted in a reduction of 24%, while the resection of both menisci lowered tan δ by 18% compared to the intact state. This biomechanical in vitro study indicates that the shock-absorbing ability of a knee joint is lower when meniscal tissue is resected. In other words, the meniscus contributes to the shock absorption of the knee joint not only during impact loads, but also during sinusoidal loads. The findings may have an impact on the rehabilitation of young, meniscectomized patients who want to return to sports. Consequently, such patients are exposed to critical loads on the articular cartilage, especially when performing sports with recurring impact loads transmitted through the knee joint surfaces.
How Social Support Impact Teachers’ Mental Health Literacy: A Chain Mediation Model

Teachers have an important social role, and their mental health literacy is very important to their own abilities as educators and to the growth and development of those they educate. This study explored the mechanism underlying the influence of social support on teachers’ mental health literacy by conducting a questionnaire survey of 573 teachers. The results showed that social support can influence teachers’ mental health literacy not only through the separate effects of life satisfaction and coping tendency but also through the chain mediation effect of life satisfaction and coping tendency; however, the direct effect of social support on the teachers’ mental health literacy is not significant. This study is conducive to understanding the internal mechanism underlying the relationship between social support and mental health literacy. It reminded us that when formulating mental health literacy promotion programs for teachers, we should not only provide adequate social support to improve but also should pay attention to improvements in their coping tendencies and life satisfaction.
Classifying Polyomavirus Nephropathy: The “Banff” Initiative

All classification systems, especially if newly designed such as the Banff classification of polyomavirus nephropathy (PyVN) (1–3), have to be further validated. In this context, we read, with great interest, the article by Kowalewska et al. “Assessment of the Banff Working Group classification of definitive BK polyomavirus nephropathy” in the November 2021 issue of Transplant International (4). We are encouraged to learn about their findings confirming aspects of the Banff 3-tier polyomavirus nephropathy classification system. We are also not too surprised to read about some differences. Based on statistical analysis, the Banff working group on polyomavirus nephropathy (here referred to as “Banff”) has identified two histologic variables, the ci and pvl scores as predictors of renal function (2, 3); those are used in the Banff system to define polyomavirus nephropathy disease classes (1). Kowalewska et al. reported similar observations (4). They also noted a significantly earlier diagnosis of PyVN in classes 1 and 2 compared to class 3. Post diagnosis all studies observed progressive deterioration of renal function in all PyVN classes, most pronounced in disease class 3. Both Banff studies (2, 3) and Kowalewska’s report (4) showed patients in disease class 3 with protracted viral resolution. Vice versa PyVN patients with disease resolution were more often found in disease classes 1 and 2. Interestingly, “Banff” (2) reported that early disease resolution indicated improved overall graft function and survival with most pronounced effects seen in class 2. Early clearance in class 2 (seen in 35% of cases) resulted in good outcome like class 1 and vice versa no clearance (in 65%) in inferior outcome like class 3 (2). Since Kowalewska et al. presumably were only able to collect a single serum creatinine data point post index biopsy at the 24-month mark, in contrast to the “Banff” reports with data collection at 1, 3, 6, 12, and 24 months, study results may not be fully comparable. However, there is general agreement among the studies that the detection of a lower PyVN class, often diagnosed early after transplantation, predicts good allograft function. In addition, early/efficient viral clearance and disease resolution are factors preserving graft integrity and stable S-Cr levels. In order to assess the impact of a PyVN diagnosis on allograft function at time of the initial index biopsy, “Banff” compared the lowest S-Cr level before diagnosis (= best preceding baseline S-Cr) with the highest one at time of index biopsy/diagnosis, i.e. the maximum delta-change. Using this approach, “Banff” noted significant differences in function at time of diagnosis that weremost pronounced in class 3. *Correspondence: Volker Nickeleit volker_nickeleit@med.unc. edu
Giant Cell Arteritis: Updates and Controversies

Abstract Giant cell arteritis (GCA) is a systemic granulomatous vasculitis affecting the medium and large-size arteries, and may present with a range of ophthalmic findings. This review will cover GCA epidemiology, pathophysiology, clinical presentation, diagnostic workup, and treatment. Epidemiology and Pathophysiology GCA is commonly found in elderly patients and individuals of Scandinavian descent. Recent publications suggest it may be more common in African Americans and Hispanics than previously thought. It is very rare in Asian and Middle-Eastern populations, and there is little data regarding African populations. Genetic studies have identified increased risk associated with HLA-DRB1*04. Rather than a response to a specific antigen such as varicella zoster virus, current immunology research suggests that GCA results from changes associated with the aging immune system. Clinical presentation to Ophthalmology Arteritic anterior ischemic optic neuropathy is the most common ophthalmic manifestation of GCA, but central or branch retinal artery occlusion, ophthalmic artery occlusion, cranial neuropathies causing diplopia, and more rarely anterior segment ischemia and anisocoria may also occur. Clinical testing including visual field testing, OCT, OCT-A, ICG and fluorescein angiography can be helpful in suggesting a diagnosis in addition to the clinical exam. Diagnostic Workup GCA is ultimately a clinical diagnosis, but it is usually supported with lab results, pathology, and/or imaging. Temporal artery biopsy (TAB) remains the gold standard diagnostic test although its sensitivity is debated and practice patterns still vary with respect to sample length and whether unilateral or simultaneous bilateral biopsies are performed. Some studies have reported higher sensitivity of ultrasounds over TAB, with added benefits of time efficiency and cost effectiveness, promoting the diagnostic use of ultrasounds. MRI and even PET CT protocols offer additional options for less invasive diagnostic testing. Treatment Vision-threatening GCA is treated acutely with emergent admission for intravenous methylprednisolone, and long-term high dose oral corticosteroids remain the standard of care, despite common and sometimes serious side effects. The use of steroid-sparing alternatives such as tocilizumab is becoming more common and additional agents are being investigated.
Room Temperature Phosphorescence of Chlorine Doped Carbon Nitride Dots

Metal free room temperature phosphorescent materials have been the subject of considerable attention due to their potential applications in optoelectronic devices sensing, and security and safety signage. This study discusses how efficient fluorescent and phosphorescent chlorine doped carbon nitride dots (Cl-CNDs) were prepared by thermal treatment of guanidine hydrochloride. The Cl-CNDs prepared were characterized by field emission scanning electron microscope, dynamic light scattering, PXRD, EDX, Thermo gravimetric analysis, FT-IR, and UV-Visible spectroscopy. The Cl-CNDs exhibit a long phosphorescence lifetime of 657 ms and the phosphorescence quantum yield was found to be 2.32% upon being excited at 360 nm in ambient conditions. Formation of compact coreparticles via condensation along with hydrogen bonding of Cl-CNDs by its functional groups facilitate intersystem crossing and stabilizes the triplet states, favoring room temperature phosphorescence. The cost effective preparation and tunable optical properties of Cl-CNDs may find applications in security encryption and optoelectronic devices.
Epigenetic Mechanism of 5-HT/NE/DA Triple Reuptake Inhibitor on Adult Depression Susceptibility in Early Stress Mice

Major depressive disorder (MDD) is a chronic, remitting and debilitating disease and the etiology of MDD is highly complicated that involves genetic and environmental interactions. Despite many pharmacotherapeutic options, many patients remain poorly treated and the development of effective treatments remains a high priority in the field. LPM570065 is a potent 5-hydroxytryptamine (5-HT), norepinephrine (NE) and dopamine (DA) triple reuptake inhibitor and both preclinical and clinical results demonstrate significant efficacy against MDD. This study extends previous findings to examine the effects and underlying mechanisms of LPM570065 on stress vulnerability using a “two-hit” stress mouse model. The “two-hit” stress model used adult mice that had experienced early life maternal separation (MS) stress for social defeat stress (SDS) and then they were evaluated in three behavioral assays: sucrose preference test, tail suspension test and forced swimming test. For the mechanistic studies, methylation-specific differentially expressed genes in mouse hippocampal tissue and ventral tegmental area (VTA) were analyzed by whole-genome transcriptome analysis along with next-generation bisulfite sequencing analysis, followed by RT-PCR and pyrophosphate sequencing to confirm gene expression and methylation. LPM570065 significantly reversed depressive-like behaviors in the mice in the sucrose preference test, the tail suspension test, and the forced swimming test. Morphologically, LPM570065 increased the density of dendritic spines in hippocampal CA1 neurons. Hypermethylation and downregulation of oxytocin receptor (Oxtr) in the hippocampal tissues along with increased protein expression of Dnmt1 and Dnmt3a in mice that experienced the “two-hit” stress compared to those that only experienced adulthood social defeat stress, and LPM570065 could reverse these changes. Combined, these results suggest that methylation specificity of the gene Oxtr in the hippocampus may play an important role in early life stress-induced susceptibility to depression and that the5-HT/NE/DA triple reuptake inhibitor LPM570065 may reduce depression susceptibility via the reversal of the methylation of the gene Oxtr.
First Assessment of Mercury (Hg) Concentrations in Skin and Carapace of Flatback Turtles (Natator depressus) (Garman) From Western Australia

Mercury pollution in the surface ocean has more than doubled over the past century. Within oceanic food webs, sea turtles have life history characteristics that make them especially vulnerable to mercury (Hg) accumulation. In this study we investigated Hg concentrations in the skin and carapace of nesting flatback turtles (Natator depressus) from two rookeries in Western Australia. A total of 50 skin samples and 52 carapace samples were collected from nesting turtles at Thevenard Island, and 23 skin and 28 carapace samples from nesting turtles at Eighty Mile Beach. We tested the influence of turtle size on Hg concentrations, hypothesising that larger and likely older adult turtles would exhibit higher concentrations due to more prolonged exposure to Hg. We compared the rookeries, hypothesising that the turtles from the southern rookery (Thevenard Island) were more likely to forage and reside in the Pilbara region closer to industrial mining activity and loading ports (potential exposure to higher environmental Hg concentrations) with turtles from the northern rookery (Eighty Mile Beach) more likely to reside and feed in the remote Kimberley. Turtles from the Eighty Mile Beach rookery had significantly higher skin Hg concentrations (x̄ = 19.4 ± 4.8 ng/g) than turtles from Thevenard Island (x̄ = 15.2 ± 5.8 ng/g). There was no significant difference in carapace Hg concentrations in turtles between Eighty Mile Beach (x̄ = 48.4 ± 21.8 ng/g) and Thevenard Island (x̄ = 41.3 ± 16.5 ng/g). Turtle size did not explain Hg concentrations in skin samples from Eighty Mile Beach and Thevenard Island, but turtle size explained 43.1% of Hg concentrations in the carapace of turtles from Eighty Mile Beach and 44.2% from Thevenard Island. Mercury concentrations in the flatback turtles sampled in this study are relatively low compared to other sea turtles worldwide, likely a result of the generally low concentrations of Hg in the Australian environment. Although we predicted that mining activities would influence flatback turtle Hg bioaccumulations, our data did not support this effect. This may be a result of foraging ground overlap between the two rookeries, or the predominant wind direction carrying atmospheric Hg inland rather than seaward. This is the first Hg study in skin and carapace of flatback turtles and represents a baseline to compare Hg contamination in Australia’s surrounding oceans.
Accuracy of a Single, Heparin-Calibrated Anti-Xa Assay for the Measurement of Rivaroxaban, Apixaban, and Edoxaban Drug Concentrations: A Prospective Cross-Sectional Study

Background Applying a single anti-Xa assay, calibrated to unfractionated heparin to measure rivaroxaban, apixaban, and edoxaban would simplify laboratory procedures and save healthcare costs. Aim We hypothesized that a heparin-calibrated anti-Xa assay would accurately measure rivaroxaban, apixaban, and edoxaban drug concentrations and correctly predict clinically relevant drug levels. Methods This analysis is part of the Simple-Xa study, a prospective multicenter cross-sectional study conducted in clinical practice. Patients treated with rivaroxaban, apixaban, or edoxaban were included. Anti-Xa activity was measured using the Siemens INNOVANCE® Heparin assay. Drug concentrations were determined using ultra-high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Cut-off levels were determined in a derivation dataset (50% of patients) and sensitivities and specificities were calculated in a verification dataset (50% of patients). Results Overall, 845 patients were available for analysis. Correlation coefficients (rs) between the heparin-calibrated anti-Xa assay and drug concentrations were 0.97 (95% CI 0.97, 0.98) for rivaroxaban, 0.96 (0.96, 0.97) for apixaban, and 0.96 (0.94, 0.99) for edoxaban. The area under the receiver operating characteristics curve (ROC) was 0.99 for all clinically relevant drug concentrations. In the verification dataset, the sensitivity was 94.2% (95% CI 90.8–96.6) for 30 μg L–1, 95.8% (92.4–98.0) for 50 μg L–1, and 98.7% (95.5–99.9) for 100 μg L–1. Specificities were 86.3% (79.2–91.7), 89.8% (84.5–93.7), and 88.7% (84.2–92.2), respectively. Conclusion In a large prospective study in clinical practice, a strong correlation of heparin-calibrated anti-Xa measurements with LC-MS/MS results was observed and clinically relevant drug concentrations were predicted correctly. Graphical Abstract Visual summary.
Diversity and Distribution of Traditional Home Gardens Along Different Disturbances in a Dry Tropical Region, India

Home gardening is an indigenous practice of cultivation that has effectively adapted to local ecological conditions over generations. This study examined the effects of disturbance and garden size on biodiversity to develop a better understanding of vegetation cover and its role in livelihood and provision of forest management in the Vindhyan highlands. Data were collected from 60 gardens which were classified into large (> 650 m2), medium (400–650 m2), and small (< 400 m2), based on size and disturbance gradients viz., high, medium, and low. A total of 133 species from 50 families were recorded, in which trees (47.4%) were dominant followed by shrubs (18%) and herbs (16.5%). With respect to disturbance, the highest number of tree species (39) were found at low disturbance (LD) followed by 33 species in medium disturbance (MD) and 32 species in high disturbhance (HD). The total mean richness of species was greater at LD (20.3 ± 2.3) and lowest at HD (18.5 ± 2.2). Tree density was significantly (P ≤ 0.05) higher at LD (293.75 ± 16.1 individual ha–1) as compared to MD (221 ± 11.5 individual ha–1) and HD (210 ± 10.3 individual ha–1). However, the results for shrubs and herbs density were considerably different, where shrubs density was highest at HD (70 ± 6.9 individual per 1,000 m2) and lowest at LD (62.5 ± 5.8 individual per 1,000 m2), while the maximum density of herbs was recorded at MD (466.25 ± 29.8 individual per 100 m2) and minimum at LD (370 ± 21.4 individual per 100 m2). The summed dominance ratio indicated frequent use of garden plants in bio-fencing, vegetables, ornamental, and ethnomedicine. Diversity (P < 0.01) and species richness (P < 0.05) showed a significant positive correlation with garden size. The Principal Component Analysis (PCA) showed that the first component (PC1) accounted for 28.6% of variance, whereas the second explained 21.9% of variance in both disturbance and garden size with a cumulative variance of 50.5%. These components depicted the positive association with HD (14.34), SDiv (13.91), TCD (12.47), and HDiv (12.09). We concluded that the diversity of home gardens changed with disturbance, which crucially served as a refuge for native tree species in a degraded landscape. This pattern highlighted the importance of home gardens for plant biodiversity conservation and local livelihood, which must be a viable option for regeneration of deforested dry tropics, while also reducing the burden on dry tropical forest regions.
Crucial Role of Stromal Interaction Molecule-Activated TRPC-ORAI Channels in Vascular Remodeling and Pulmonary Hypertension Induced by Intermittent Hypoxia

Obstructive sleep apnea (OSA), a sleep breathing disorder featured by chronic intermittent hypoxia (CIH), is associate with pulmonary hypertension. Rats exposed to CIH develop lung vascular remodeling and pulmonary hypertension, which paralleled the upregulation of stromal interaction molecule (STIM)-activated TRPC-ORAI Ca2+ channels (STOC) in the lung, suggesting that STOC participate in the pulmonary vascular alterations. Accordingly, to evaluate the role played by STOC in pulmonary hypertension we studied whether the STOC blocker 2-aminoethoxydiphenyl borate (2-APB) may prevent the vascular remodeling and the pulmonary hypertension induced by CIH in a rat model of OSA. We assessed the effects of 2-APB on right ventricular systolic pressure (RVSP), pulmonary vascular remodeling, α-actin and proliferation marker Ki-67 levels in pulmonary arterial smooth muscle cells (PASMC), mRNA levels of STOC subunits, and systemic and pulmonary oxidative stress (TBARS) in male Sprague-Dawley (200 g) rats exposed to CIH (5% O2, 12 times/h for 8h) for 28 days. At 14 days of CIH, osmotic pumps containing 2-APB (10 mg/kg/day) or its vehicle were implanted and rats were kept for 2 more weeks in CIH. Exposure to CIH for 28 days raised RVSP > 35 mm Hg, increased the medial layer thickness and the levels of α-actin and Ki-67 in PASMC, and increased the gene expression of TRPC1, TRPC4, TRPC6 and ORAI1 subunits. Treatment with 2-APB prevented the raise in RVSP and the increment of the medial layer thickness, as well as the increased levels of α-actin and Ki-67 in PASMC, and the increased gene expression of STOC subunits. In addition, 2-APB did not reduced the lung and systemic oxidative stress, suggesting that the effects of 2-APB on vascular remodeling and pulmonary hypertension are independent on the reduction of the oxidative stress. Thus, our results supported that STIM-activated TRPC-ORAI Ca2+ channels contributes to the lung vascular remodeling and pulmonary hypertension induced by CIH.
LC_Glucose-Inhibited Division Protein Is Required for Motility, Biofilm Formation, and Stress Response in Lysobacter capsici X2-3

Glucose-inhibited division protein (GidA) plays a critical role in the growth, stress response, and virulence of bacteria. However, how gidA may affect plant growth-promoting bacteria (PGPB) is still not clear. Our study aimed to describe the regulatory function of the gidA gene in Lysobacter capsici, which produces a variety of lytic enzymes and novel antibiotics. Here, we generated an LC_GidA mutant, MT16, and an LC_GidA complemented strain, Com-16, by plasmid integration. The deletion of LC_GidA resulted in an attenuation of the bacterial growth rate, motility, and biofilm formation of L. capsici. Root colonization assays demonstrated that the LC_GidA mutant showed reduced colonization of wheat roots. In addition, disruption of LC_GidA showed a clear diminution of survival in the presence of high temperature, high salt, and different pH conditions. The downregulated expression of genes related to DNA replication, cell division, motility, and biofilm formation was further validated by real-time quantitative PCR (RT–qPCR). Together, understanding the regulatory function of GidA is helpful for improving the biocontrol of crop diseases and has strong potential for biological applications.