Miracle-dz/UniFAR-FTUs huggingface dataset:
Immunoreactive Response of Plast-MIPs to Fasting and Their Functional Role in the Reduction of Hemolymph Reducing Sugars in the Brown-Winged Green Bug, Plautia stali

Animals survive nutrient deficiency by controlling their physiology, such as sugar metabolism and energy-consuming developmental events. Although research on the insect neural mechanisms of the starvation-induced modulation has progressed, the mechanisms have not been fully understood due to their complexity. Myoinhibitory peptides are known to be neuropeptides involved in various physiological activities, development, and behavior. Here, we analyzed the responsiveness of Plautia stali myoinhibitory peptides (Plast-MIPs) to starvation and their physiological role in the brown-winged green bug, P. stali. First, we performed immunohistochemical analyses to investigate the response of Plast-MIP neurons in the cephalic ganglion to fasting under long day conditions. Fasting significantly enhanced the immunoreactivity to Plast-MIPs in the pars intercerebralis (PI), which is known to be a brain region related to various endocrine regulations. Next, to analyze the physiological role of Plast-MIPs, we performed RNA interference-mediated knockdown of Plast-Mip and injection of synthetic Plast-MIP in normally fed and fasted females. The knockdown of Plast-Mip did not have significant effects on the body weight or proportions of ovarian development in each feeding condition. On the other hand, the knockdown of Plast-Mip increased the gonadosomatic index of normally fed females whereas it did not have a significant effect on food intake. Notably, the knockdown of Plast-Mip diminished the fasting-induced reduction of hemolymph reducing sugar levels. Additionally, injection of synthetic Plast-MIP acutely decreased the hemolymph reducing sugar level. Our results suggested responsiveness of Plast-MIPs in the PI to fasting and their functional role in reduction of the hemolymph reducing sugar level.
Two Decades of Game Jams

In less than a year’s time, March 2022 will mark the twentieth anniversary of the first documented game jam, the Indie Game Jam, which took place in Oakland, California in 2002. Initially, game jams were widely seen as frivolous activities. Since then, they have taken the world by storm. Game jams have not only become part of the day-to-day process of many game developers, but jams are also used for activist purposes, for learning and teaching, as part of the experience economy, for making commercial prototypes that gamers can vote on, and more. Beyond only surveying game jams and the relevant published scientific literature from the last two decades, this paper has several additional contributions. It builds a history of game jams, and proposes two different taxonomies of game jams — a historical and a categorical. In addition, it discusses the definition of game jam and identifies the most active research areas within the game jam community such as the interplay and development with local communities, the study and analysis of game jammers and organisers, and works that bring a critical look on game jams.
Phytosynthesis of Iron Nanoparticle from Averrhoa Bilimbi Linn.

This paper demonstrates iron nanoparticles (FeNP) was synthesized from natural sources of Averrhoa bilimbi Linn. The plant extracts act as natural reducing agent in producing FeNP. There is no addition of any surfactants during the nanoparticles formation. Gravimetric analysis is used to calculate the percentage yield of plant extracts. TPC and DPPH assay method were used to evaluate antioxidant activity in different A. bilimbi extracts and synthesized FeNP. Based on the analyses, it showed that fruit has the highest percentage yield and antioxidant activity followed by leaf, twig and bark. Analysis from TPC, fruit contains 27.26 mg GAE/g and 39.46 mg GAE/g for FeNP. DPPH assay showed fruit extract has the highest free radical antioxidant activity with 61.93% in A. bilimbi and 80.00% in FeNP. Phytosynthesis of FeNP were examine by using UV-Vis spectrophotometer. Based on the spectra, it showed that FeNP recorded peak absorbance at 465 nm, 450 nm, 460 nm and 440 nm for UAE-F, UAE-L, UAE-T and UAE-B, respectively. FTIR analysis shows the presence of strong alcoholic bond, aldehyde, stretch amine and alkene that was responsible in reduction process to form FeNP. The result of UV-Vis and FTIR showed that the existance of FeNP and involvement of functional group that were responsible on the formation of nanoparticles.
Long-Term Temporal Dynamics of Synaptic Vesicles

Neurotransmission requires the release of neurotransmitters from synaptic vesicles. This occurs via fusion of the vesicle to the pre-synaptic membrane upon stimulation. However, not all synaptic vesicles are equally releasable, and it has long been debated why the majority of synaptic vesicles do not respond to physiological levels of stimulation. I demonstrate here, using live-cell antibody-tagging in rat hippocampal cultures, that only young synaptic vesicles are releasing neurotransmitter, and that they become more reluctant to release as they age. This inactivation of synaptic vesicles is not strictly an ageing-dependent process, but conditional upon vesicle usage. I report here that synaptic vesicles release ~260 times, on average, before becoming inactive, and that increasing usage frequency speeds up inactivation. The inactivation is caused by contamination of synaptic vesicles with the cell membrane protein SNAP25. SNAP25 can interact with the vesicle protein CSPα in cis- complexes on the vesicle itself. This sequesters CSPα and prevents the formation of trans- complexes with SNAP25 on the cell membrane. This trans-interaction, however, promotes vesicle fusion to the cell membrane. The more often a vesicle has fused to the cell membrane, the higher its chance is to be contaminated with SNAP25, and the less competent it is for future rounds of release. The inactivation of ageing synaptic vesicles is presumably coupled to usage to remove potentially damaged synaptic vesicles from neurotransmission. This hypothesis is strengthened by the observation of endocytosis defects and neurite degeneration when aged vesicles are forced to release. I further provide several timing parameters for key events in the life of synaptic vesicles, which can serve as a framework towards a quantitative model of the synaptic vesicle life cycle.
On water waves generated by a bottom obstacle translating at a subcritical speed

Abstract This study investigates water waves generated by a bottom obstacle translating at a subcritical speed in constant water depth, using a combination of analytical and numerical approaches. The newly derived analytical solutions reveal two types of waves – the transient free waves that propagate radially outwards, and the trapped wave that stays on top of the translating bottom obstacle. Closed-form asymptotic solutions for both the free surface and the flow velocities are derived in the far field, and near the leading wave or in the shallow water limit. The far-field leading waves are mathematically shown to be insensitive to the exact shape of the obstacle. Numerical long-wave models are employed to examine effects unaccountable by the linear analytical solutions. Nonlinear effects are found to cause only small deviations from the linear solutions. The effects of the obstacle's acceleration and deceleration are also examined numerically. Overall, the idealised linear analytical solutions predict well the characteristics of water waves generated by a bottom obstacle, and therefore can serve as the cornerstone of a theory-based model for quickly predicting the tsunamis generated by a submarine landslide.
Removal of Hg2+ and methylmercury in waters by functionalized multi-walled carbon nanotubes: adsorption behavior and the impacts of some environmentally relevant factors

Abstract Adsorption of Hg2+ and methylmercury (MeHg) to multi-walled carbon nanotubes (MWCNTs) modified, respectively, with hydroxyl, amine and carboxyl groups was studied. The effect of various factors like the initial pH, natural organic matter (NOM), Cl- and adsorbent dose on the sorption efficiency were evaluated. It was found that amine-modified MWCNTs showed a strong adsorption capacity to Hg2+ and MeHg, and the removal efficiency could reach up to 92% (0.5 g/L MWCNTs, and 100 μg/L Hg2+ and MeHg) which is independent of pH. NOM had complex effects on the adsorption of Hg2+ and MeHg to MWCNTs. Cl- inhibited the adsorption of Hg2+ and MeHg to MWCNTs. The adsorption of Hg2+ and MeHg was found to be inhomogeneous and homogeneous chemisorption, respectively. Our results suggested that MWCNTs modified with different functional groups can efficiently adsorb both Hg2+ and MeHg in aqueous environment.
Age and Moral Foundations: A Meta-Analytic Approach

Abstract Several meta-analytic analyses are carried out to analyzed the relationship between age and different moral constructs based on the Moral Foundations Theory (MFT) framework. Pearson’s correlation estimates between age and any of the moral construcs were available for a total of 239 independent samples out of 122 studies. Correlation coefficients were meta-analyzed, heterogeneity was examined by searching for moderators when there were more than 30 estimates available, and a predictive model to estimate the expected correlation was proposed when several moderators showed a significant effect. The correlation between age and all the moral constructs analyzed exhibited pooled estimates of null or not relevant magnitude, ranging from –. 02 to . 08. The moderator analyses led to a predictive model in which participant’s mean age and ideology explained 40.80% of the total variability among the correlation between age and the Loyalty/Betrayal foundation, whereas participant’s mean age explained a significant percentage of variability (8.85 – 25.12%) for the correlations between age and the rest of moral foundations and the Individualizing group. Results show a quite stable moral matrix over the lifespan, but future research is needed for examine a possible non-linear relationship between age and moral foundations.
Predictive modeling reveals that higher-order cooperativity drives transcriptional repression in a synthetic developmental enhancer

A challenge in quantitative biology is to predict output patterns of gene expression from knowledge of input transcription factor patterns and from the arrangement of binding sites for these transcription factors on regulatory DNA. We tested whether widespread thermodynamic models could be used to infer parameters describing simple regulatory architectures that inform parameter-free predictions of more complex enhancers in the context of transcriptional repression by Runt in the early fruit 2y embryo. By modulating the number and placement of Runt binding sites within an enhancer, and quantifying the resulting transcriptional activity using live imaging, we discovered that thermodynamic models call for higher-order cooperativity between multiple molecular players. This higher-order cooperativity capture the combinatorial complexity underlying eukaryotic transcriptional regulation and cannot be determined from simpler regulatory architectures, highlighting the challenges in reaching a predictive understanding of transcriptional regulation in eukaryotes and calling for approaches that quantitatively dissect their molecular nature.
Mechanism of Antiviral Activity of Nitazoxanide against the Influenza Virus: Effect of Tizoxanide on AdenosineTriphosphate in Influenza-virus Infected Madin Darby Canine Kidney Cells

Background Nitazoxanide (NTZ) is a broad-spectrum antiviral undergoing clinical development for treating influenza and other viral respiratory infections such as those caused by rhinovirus/enterovirus and coronavirus including the emerging SARS-CoV-2. Methods Nitazoxanide is a mild uncoupler of oxidative phosphorylation, which is modulating the ATP production in cells. ATP is an essential component of viral replication, and we have evaluated the effect of tizoxanide (TIZ), the active circulating metabolite of NTZ, on ATP in Madin-Darby canine kidney (MDCK) cells and in MDCK cells infected with influenza A and B viruses. Results TIZ decreased cellular ATP in a dose-dependent manner in MDCK cells and in MDCK cells infected with influenza A and B viruses. Maximum inhibition of ATP in influenza infected or uninfected MDCK cells reached up to 45% after 6 and 24 hours of exposure to 100µM TIZ. The decrease in cellular ATP did not affect cell viability and was reversible after eliminating TIZ from the culture. Conclusion The concentrations of TIZ required to decrease cellular ATP levels were similar to those reported to inhibit replication of influenza A and B viruses in our laboratory. A decrease in ATP triggers activation of AMP-activated protein kinase, which is known to suppress the secretion of pro-inflammatory cytokines. Additional studies are warranted to evaluate the effect of TIZ on mitochondrial function.
Macau residents’ attitude towards the free independent travellers (FIT) policy: an analysis from the perspective of the ABC model and group comparison

ABSTRACT This study analyses the formation mechanism of residents' support intentions using the affect, behaviour, and cognition (ABC) model of attitudes and structural equation modelling. The findings indicate that residents' satisfaction with tourism development is primarily motivated by favourable perceptions. Negative perceptions such as perceived social cost and perceived economic cost have no significant influence on residents' satisfaction, whereas satisfaction has a significant influence on their support intentions. The results of the multi-group analysis indicate that there are significant differences in the characteristics of different resident groups in terms of support formation.
Decentralized cooperative driving automation: a reinforcement learning framework using genetic fuzzy systems

Cooperative Automated Driving System (ADS)-equipped Vehicles (CoAVs) can be a promising solution to traffic congestion. We present a reinforcement learning strategy using Genetic Fuzzy Systems (GFS) for cooperative merge of vehicles onto a highway in high density, mixed-autonomy traffic conditions. The CoAVs are trained to make their own decisions purely based on information available from its surrounding vehicles, thus making this a decentralized system. The GFS module in each CoAV makes recommendations on the acceleration and lane-change decisions. The CoAVs are trained on different ADS behavioral parameters, such as aggressiveness of lane change, different CoAV market penetration rates (MPRs) and traffic congestion levels. The results show the effectiveness of increasing the MPR. It is noticed that the CoAVs trained with different ADS behavioral parameters can generate completely different cooperative maneuvers. The results also show that the trained CoAVs can cooperate even with human-driven vehicles to improve the overall traffic performance.
Evaluation of Community Structures and their Physicochemical Correlation with Five Hot Springs in India

Abstract Thermal springs have been the most resourceful ecological niches to understand the intricacies of the microbial community structure building. In the present study, the microbial community structure was investigated in five ecologically different hot springs. The highest number of OTUs was observed at low temperatures (42 °C) whereas an increasing number of unclassified bacteria was observed with a temperature rise. The statistical correlation predicted that temperature, total dissolved solids and ions were the primary environmental factors in controlling the community composition and diversity. The relative abundance of Proteobacteria, Cyanobacteria showed a positive correlation with moderate temperature whereas growth of Chloroflexi and Nitrospira was unstable at 65 °C. The observed LCBD was negatively correlated to the bacteria richness. A high relative abundance of Planctomycetes was restricted to Odisha hot springs (AT, TP, and DJ). We further hypothesize that abundance of most common cellulose-degrading bacteria such as Acinetobacter, Pseudomonas and Aeromonas only in DJ hot spring is possibly due to the high carbon content of the runoff water received from dense pandanus forest around it and could be a prospective source of industrially relevant cellulase after detailed characterization. The present study concludes that the association of physicochemical components with key species drive the microbial community structure.
Comparison of teacher agency for collaboration within and across curriculum teams

ABSTRACT Teacher agency plays a vital role in professional learning. The revealing practice indicates its fluidity in the process of collaboration. Research suggests that learning through interaction happens by engaging teachers. However, the role of teacher agency remains unclear at a systemic level. This article reports a yearlong ethnographic study of overlapping members from two teams collaborating on contemporary liberal arts curriculum across two different education systems in a Hong Kong secondary school. Discourse analysis of collaborative meetings demonstrated that the overlapping members performed various functions in the two settings. The connections and disconnections suggested that teacher agency was conditional based on the ecological boundary. By identifying their patterns of practice, individual teachers collectively demonstrated different capacities in the two settings within and over time. The findings imply that collaboration is an embedded activity and strongly influenced by the school environment. Overall, this study contributes to a reconceptualisation of professional learning.
Ethics and standards in the use of artificial intelligence in medicine on behalf of the Royal Australian and New Zealand College of Radiologists

The Royal Australian and New Zealand College of Radiologists (RANZCR) led the medical community in Australia and New Zealand in considering the impact of machine learning and artificial intelligence (AI) in health care. RANZCR identified that medical leadership was largely absent from these discussions, with a notable absence of activity from governments in the Australasian region up to 2019. The clinical radiology and radiation oncology sectors were considered ripe for the adoption of AI, and this raised a range of concerns about how to ensure the ethical application of AI and to guide its safe and appropriate use in our two specialties.
Identification and Phylogenetic Analyses of Anaerobic Sulfidogenic Bacteria in Two Algerian Oilfield Water Injection Samples

Abstract Corrosion of metallic oilfield pipelines by microorganisms is a costly but poorly understood phenomenon. For the first time, sulfidogenic communities in injection waters of two Algerian oilfields, Tin Fuin Tabankort (IT3) and Stah (IS2) were examined using the 16S rRNA gene cloning and sequencing approach. Water samples were inoculated into selective medium for sulfate-reducing bacteria and incubated under anaerobic conditions at 45 °C. The total number of culturable sulfidogenic microorganisms in the samples obtained from the two sampled waters (IT3 and IS2) was 2.4 × 105 cells/mL and 3.9 × 104 cells/mL, respectively. Scanning electron microscopy analyses showed different morphological forms reflecting the diversity of sulfidogenic communities. 16S rRNA gene sequencing and phylogenetic diversity analyses revealed that both water reservoirs harbor large amounts of anaerobic bacteria. However, a majority of all the sequences analyzed (e.g., 34% in the IS2 and 84% in the IT3 samples) were not assigned to any known bacterial group, suggesting that subsurface waters harbor very large sulfidogenic anaerobic microbial communities of as yet undescribed bacterial phyla. Proteobacteria were found to be the most dominant phylum in the IS2 sample (49%); however, no Proteobacteria were detected at the IT3 production well. The Firmicutes phylum (10%) was detected in the two water samples, whereas Bacteroidetes phylum (7%) was retrieved only in IT3. The most abundant related genera were: Desulfotomaculum, Porphyrobacter, Hyphomicrobium, Acidocella, Comamonas, Ramlibacter, Pseudomonas, Enterobacter and Flavitalea. No shared operational taxonomic units were observed among the two samples analyzed, demonstrating the uniqueness of each subsurface water well. This study demonstrates the diversity of the sulfidogenic bacteria that might play a critical role in the souring mediated corrosion of metallic oilfield pipelines. This information could help oilfield companies develop better anticorrosion treatments and strategies.
The awe-prosociality relationship: evidence for the role of context

ABSTRACT People in a state of awe have been found to perceive their needs as small while also expressing intentions to act in a prosocial way, benefitting others at personal cost. However, these findings come largely out of the USA and have focused on intended rather than real prosocial behavior. We propose a contextual model of the awe-prosociality relationship predicated on the constructed theory of emotion, according to which emotion categories and cost–benefit analyses of possible subsequent actions differ across cultures and in line with enduring individual differences. To test the model, we conducted a laboratory study (N = 143) examining whether costly volunteering behavior is higher amid awe in the Czech Republic, a country where social psychological studies have often produced different results compared to the USA. Awe-inspiring and neutral primes were validated in pilot studies (N = 229). As is possible under the contextual model, awe-inspiring primes elicited not more, but less, prosocial behavior, with the relationship being moderated by various facets of Openness to Experience. Individuals higher in the Feelings facet of Openness were also found to be more awe-prone. A call is made for a cross-cultural investigation of the awe-behavior relationship that accounts for complex phylogenetic relationships between cultures.
Selection in males purges the mutation load on female fitness

Theory predicts that the ability of selection and recombination to purge mutation load is enhanced if selection against deleterious genetic variants operates more strongly in males than females. However, direct empirical support for this tenet is limited, in part because traditional quantitative genetic approaches allow dominance and intermediate‐frequency polymorphisms to obscure the effects of the many rare and partially recessive deleterious alleles that make up the main part of a population's mutation load. Here, we exposed the partially recessive genetic load of a population of Callosobruchus maculatus seed beetles via successive generations of inbreeding, and quantified its effects by measuring heterosis—the increase in fitness experienced when masking the effects of deleterious alleles by heterozygosity—in a fully factorial sex‐specific diallel cross among 16 inbred strains. Competitive lifetime reproductive success (i.e., fitness) was measured in male and female outcrossed F1s as well as inbred parental “selfs,” and we estimated the 4 × 4 male‐female inbred‐outbred genetic covariance matrix for fitness using Bayesian Markov chain Monte Carlo simulations of a custom‐made general linear mixed effects model. We found that heterosis estimated independently in males and females was highly genetically correlated among strains, and that heterosis was strongly negatively genetically correlated to outbred male, but not female, fitness. This suggests that genetic variation for fitness in males, but not in females, reflects the amount of (partially) recessive deleterious alleles segregating at mutation‐selection balance in this population. The population's mutation load therefore has greater potential to be purged via selection in males. These findings contribute to our understanding of the prevalence of sexual reproduction in nature and the maintenance of genetic variation in fitness‐related traits.