Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessment of the physiological vulnerability of the endemic and critically endangered Daggernose Shark: A comparative approach to other Carcharhiniformes

Introduction The current Isogomphodon oxyrhynchus (Daggernose Shark) population status Q7points to 99% losses in the last decade due to certain biological traits, site fidelity, and historical high representativeness as bycatch in artisanal fisheries. This species is listed as Critically Endangered (CR), both in the IUCN and the Brazilian Red Lists. Its vulnerability is so high that its recovery potential requires protection from ongoing fishing pressure. Objective In this context, this study aimed to evaluate the health status of Daggernose sharks and their ability to cope with allostatic overload in a comparative analysis with other Carcharhinid and Sphyrnid sharks. Methods Sharks incidentally caught by the artisanal fleet in the state of Maranhão, on the Brazilian Amazon Coast, were sampled for blood, and serum was used to assess biochemical markers. Results The findings indicate significant differences in Daggernose Shark homeostatic capacity for ALP, ALT/GTP, creatinine, lactate, urea, total cholesterol, and triglycerides, pointing to lower health scores and recovery capacity when compared to other Carcharhiniformes inhabiting the same region. Discussion and conclusions It is possible that such vulnerability is a result of fisheries-induced evolution, leading to remaining populations with very low chances of fully recovering. Conservation planning is thus urgent, as current legislation based on fishing bans does very little for the species. International collaboration and longterm recovery measures are necessary, including the creation of MPAs specially designed for the species and captive maintenance aiming to monitor health status and carry out breeding attempts.
Ambulatory blood pressure monitoring in children: A retrospective single-center study

Objectives (1) Compare 24-hour ambulatory blood pressure monitoring (ABPM) diagnoses in a pediatric population with the new 2022 guidelines to the original diagnoses with the 2014 guidelines. (2) Determine whether findings of hypertension from ABPM could be predicted from prior patient data. (3) Determine whether ABPM readings could predict left ventricular mass index (LVMI) in patients who obtained an echocardiogram (ECHO). Study design Single-center retrospective study on patients referred to Pediatric Nephrology Clinic for evaluation of elevated blood pressure who underwent ABPM from 2015 to 2018. Predictions of hypertension were obtained using a logistic regression model, and predictions of LVMI were performed using regression models including (a) the wake systolic and diastolic BP indices, or (b) additionally including the standard deviation (SD) of wake SBP and DBP. Results With the change in 2022 to new ABPM guidelines from the AHA, comparing the old and new guidelines led to 70% of previous pre-hypertensive diagnoses now meeting criteria for diagnosis of hypertension, and a rise from 21% of the ABPMs meeting criteria for hypertension to 51% now meeting criteria. In a logistic regression model, prior patient data were not predictive of a diagnosis of hypertension from ABPM (Nagelkerke's R2 = 0.04). Among the individual variables studied, none were statistically significant. For prediction of LVMI, the SD of wake SBP and DBP were significantly associated with increased LVMI, but the wake SBP and DBP indices were not. Conclusions In our patient population, the new ABPM guidelines led to a significant increase in diagnoses of hypertension. Prior patient data was not sufficient to predict a diagnosis of hypertension by ABPM, supporting the need for evaluation by ABPM as the gold standard. Our analysis of the relationship between ABPM readings and LVMI supports the hypothesis that BP variability contributes to increased LVMI. These data are consistent with growing evidence in the adult literature that BP variability detected by ABPM is associated with left-ventricular hypertrophy
Can cereal-legume intercrop systems contribute to household nutrition in semi-arid environments: A systematic review and meta-analysis

Introduction Intercropping cereals with legumes can intensify rainfed cereal monocropping for improved household food and nutritional security. However, there is scant literature confirming the associated nutritional benefits. Methodology A systematic review and meta-analysis of nutritional water productivity (NWP) and nutrient contribution (NC) of selected cereal-legume intercrop systems was conducted through literature searches in Scopus, Web of Science and ScienceDirect databases. After the assessment, only nine articles written in English that were field experiments comprising grain cereal and legume intercrop systems were retained. Using the R statistical software (version 3.6.0), paired t-tests were used to determine if differences existed between the intercrop system and the corresponding cereal monocrop for yield (Y), water productivity (WP), NC, and NWP. Results The intercropped cereal or legume yield was 10 to 35% lower than that for the corresponding monocrop system. In most instances, intercropping cereals with legumes improved NY, NWP, and NC due to their added nutrients. Substantial improvements were observed for calcium (Ca), where NY, NWP, and NC improved by 658, 82, and 256%, respectively. Discussion Results showed that cereal-legume intercrop systems could improve nutrient yield in water-limited environments. Promoting cereal- legume intercrops that feature nutrient-dense legume component crops could contribute toward addressing the SDGs of Zero Hunger (SDG 3), Good Health and Well-3 (SDG 2) and Responsible consumption and production (SDG 12).
Sequential multiphoton multiple ionization of atomic argon and xenon irradiated by x-ray free-electron laser pulses from SACLA

We have investigated multiphoton multiple ionization of argon and xenon atoms at 5 keV using a new x-ray free electron laser (XFEL) facility, the SPring-8 Angstrom Compact free electron LAser (SACLA) in Japan. The experimental results are compared with the new theoretical results presented here. The absolute fluence of the XFEL pulse has been determined with the help of the calculations utilizing two-photon processes in the argon atom. The high charge states up to +22 observed for Xe in comparison with the calculations point to the occurrence of sequential L-shell multiphoton absorption and of resonance-enabled x-ray multiple ionization.
High aspect ratio nanomaterial-induced macrophage polarization is mediated by changes in miRNA levels

Introduction Inhalation of nanomaterials may induce inflammation in the lung which if left unresolved can manifest in pulmonary fibrosis. In these processes, alveolar macrophages have an essential role and timely modulation of the macrophage phenotype is imperative in the onset and resolution of inflammatory responses. This study aimed to investigate, the immunomodulating properties of two industrially relevant high aspect ratio nanomaterials, namely nanocellulose and multiwalled carbon nanotubes (MWCNT), in an alveolar macrophage model. Methods MH-S alveolar macrophages were exposed at air-liquid interface to cellulose nanocrystals (CNC), cellulose nanofibers (CNF) and two MWCNT (NM-400 and NM-401). Following exposure, changes in macrophage polarization markers and secretion of inflammatory cytokines were analyzed. Furthermore, the potential contribution of epigenetic regulation in nanomaterial-induced macrophage polarization was investigated by assessing changes in epigenetic regulatory enzymes, miRNAs, and rRNA modifications. Results Our data illustrate that the investigated nanomaterials trigger phenotypic changes in alveolar macrophages, where CNF exposure leads to enhanced M1 phenotype and MWCNT promotes M2 phenotype. Furthermore, MWCNT exposure induced more prominent epigenetic regulatory events with changes in the expression of histone modification and DNA methylation enzymes as well as in miRNA transcript levels. MWCNT-enhanced changes in the macrophage phenotype were correlated with prominent downregulation of the histone methyltransferases Kmt2a and Smyd5 and histone deacetylases Hdac4, Hdac9 and Sirt1 indicating that both histone methylation and acetylation events may be critical in the Th2 responses to MWCNT. Furthermore, MWCNT as well as CNF exposure led to altered miRNA levels, where miR-155-5p, miR-16-1-3p, miR-25-3p, and miR-27a-5p were significantly regulated by both materials. PANTHER pathway analysis of the identified miRNA targets showed that both materials affected growth factor (PDGF, EGF and FGF), Ras/MAPKs, CCKR, GnRH-R, integrin, and endothelin signaling pathways. These pathways are important in inflammation or in the activation, polarization, migration, and regulation of phagocytic capacity of macrophages. In addition, pathways involved in interleukin, WNT and TGFB signaling were highly enriched following MWCNT exposure. Conclusion Together, these data support the importance of macrophage phenotypic changes in the onset and resolution of inflammation and identify epigenetic patterns in macrophages which may be critical in nanomaterial-induced inflammation and fibrosis.
Meta-analysis of neoadjuvant immunotherapy for non-metastatic colorectal cancer

Background Immunotherapy has been approved for the treatment of metastatic colorectal cancer. The efficacy and safety of neoadjuvant immunotherapy for the treatment of non-metastatic colorectal cancer remains unclear. We tried to explore clinical effect of neoadjuvant immunotherapy in the treatment of non-metastatic colorectal cancer. Methods We searched the databases (PubMed, Wanfang Embase, Cochrane Library and China National Knowledge Infrastructure databases) to obtain suitable articles up to September 2022. The primary outcomes of pathological complete response (pCRs), major pathological response (MPR), objective response rate (ORR), R0-resection and anus preserving rate were collected and evaluated. Secordary outcomes (pCRs and MPR) of subgroup analysis between deficient mismatch repair/microsatellite instability-high group (dMMR/MSI-H) and proficient mismatch repair/microsatellite stable group (pMMR/MSS) and outcomes for rectal cancer were analyzed for the final results. Results We included ten articles and 410 cases of non-metastatic colorectal cancer with neoadjuvant immunotherapy. There were 113 (27.5%) cases with the dMMR/MSI-H status and 167 (40.7%) cases with the pMMR/MSS status. pCRs was found in 167/373 (44.6%) patients (ES: 0.49, 95% CI: 0.36 to 0.62, P<0.01, chi2 = 65.3, P<0.01, I 2 = 86.2%) and MPR was found in 194/304 (63.8%) patients (ES: 0.66, 95% CI: 0.54 to 0.78, P<0.01, chi2 = 42.55, P<0.01, I 2 = 81.2%) with the random-effects model and huge heterogeneity. In the subgroup analysis, pCRs was higher in the dMMR/MSI-H group than the pMMR/MSS group in the fixed-effects model with minimal heterogeneity (OR: 3.55, 95% CI: 1.74 to 7.27, P<0.01, chi2 = 1.86, P=0.6, I 2 = 0%). pCRs was found in 58/172 (33.9%) rectal cancer patients (ES: 0.33, 95% CI: 0.26 to 0.40, P<0.01, chi2 = 3.04, P=0.55, I 2 = 0%) with the fixed-effects model and little heterogeneity. Conclusion Neoadjuvant immunotherapy could increase pCRs and MPR rate for non-metastatic colorectal cancer. Neoadjuvant immunotherapy could achieve better pCRs rate in dMMR/MSI-H group than in the pMMR/MSS group. Neoadjuvant immunotherapy could be another treatment option for non-metastatic colorectal cancer. Systematic review registration https://www.crd. york.ac. uk/prospero/#myprospero, identifier CRD42022350523.
Risk factors and sequelae of epidermolysis bullosa acquisita: A propensity-matched global study in 1,344 patients

Identification of risk factors and sequelae of any given disease is of key importance. For common diseases, primary prevention and disease management are based on this knowledge. For orphan diseases, identification of risk factors and sequelae has been challenging. With the advent of large databases, e.g., TriNetX, this can now be addressed. We used TriNetX to identify risk factors and sequelae of epidermolysis bullosa acquisita (EBA), a severe and orphan autoimmune disease. To date, there is only enigmatic information on EBA comorbidity. We recruited 1,344 EBA patients in the Global Collaborative Network of TriNetX. Using the “explore outcomes” function we identified 55 diagnoses with a different prevalence between EBA and no-EBA patients. We next performed propensity-matched, retrospective cohort studies in which we determined the risk of EBA development following any of the identified 55 diseases. Here, 31/55 diseases were identified as risk factors for subsequent EBA. Importantly, the highest risk for EBA were other chronic inflammatory diseases (CID), especially lupus erythematosus and lichen planus. Lastly, we determined the risk to develop any of the identified diseases after EBA diagnosis. Here, 38/55 diseases were identified as sequelae. Notably, EBA patients showed an increased risk for metabolic and cardiovascular disease, and thrombosis. Furthermore, the risk for CIDs, especially lupus erythematosus and lichen planus, was elevated. These insights into risk factors and sequelae of EBA are not only of clinical relevance, e.g., optimizing cardiovascular disease risk, but in addition, point to shared pathogenetic pathways between EBA and other inflammatory diseases.
Preadolescent exposure to a sexually mature, unrelated male rat reduces postadolescent social recognition memory and CA2 c-Fos labeling

Introduction Social memory involves social recognition: the ability to discriminate between two or more conspecifics when one has been previously encountered. The CA2 region of the hippocampus has been implicated in social memory, as lesions and dysfunction to this area lead to social memory impairments. A variety of psychogenic manipulations during postnatal sensitive developmental periods are associated with social memory impairments later in life. Methods In this study, we exposed preadolescent rats to a sexually, mature unrelated male and examined whether this was associated with changes in postadolescent social memory and c-Fos labeling in the CA2 region. Male and female Long-Evans rats were exposed to a male, adult rat on postnatal days 19–21 (P19–21). Social memory was measured during the postadolescent period and defined as increased interactions towards a novel age-matched rat in contrast to a previously-encountered age-matched rat. After the test, rats were euthanized and brain tissue was then collected to quantify c-Fos labeling within the CA2 region. Results Compared to home cage controls and controls not exposed to the adult male, male and female rats exposed to the unrelated adult during preadolescence were unable to discriminate between a novel and previously encountered conspecific during the postadolescent test showing social memory deficits. The groups that showed social recognition deficits also had significantly fewer c-Fos-positive cells within the CA2 region compared to the control groups. Discussion These findings indicate that threatening psychogenic encounters during preadolescence can have detrimental long-term effects on social memory potentially via disrupted activity in the CA2 hippocampal region.
Longitudinal assessment of the temporal stability and predictive validity of the Revised Paranormal Belief Scale

The Revised Paranormal Belief Scale (RPBS) is the prevailing measure of supernatural credence. However, there exists only limited evidence to support the temporal stability and predictive validity of the instrument over time. Acknowledging this, the present study assessed the test–retest reliability of the RPBS using a large, heterogeneous sample across multiple trials. In addition, predictive validity was tested using a longitudinal statistical model, which focused on allied health outcomes (Perceived Stress and Somatic Complaints). A sample of 1,665 (Mage = 54.40, 853 females, 804 males, five non-binary and three not disclosing of gender) completed study measures at three time points separated by 2 month intervals. Prior to assessing temporal stability, assessment of structural validity and longitudinal invariance occurred. Test–retest reliability of the RPBS was in the moderate to high range across time intervals, and good internal consistency was observed. Furthermore, satisfactory stability coefficients existed for RPBS subfactors. Data-model fit for the predictive model was acceptable. Belief in the paranormal explained low variance over time in Perceived Stress and Somatic Complaints (between 2.4 and 4.2%). Findings supported the stability and reliability of the RPBS. In addition, they aligned with the notion that paranormal belief in the absence of high scores on cognitive-perceptual factors (e.g. transliminality and schizotypy), has a benign influence on perceived health.
Influence of Caribbean eddies on the Loop current system evolution

The Loop Current (LC) system dynamics are an essential component of the processes influencing circulation and transport in the Gulf of Mexico (GoM). The LC evolution is influenced by various factors, including the rich eddy field of the region and the flow exchange through the Yucatan Strait with the neighboring Caribbean Sea. These factors contribute to the complexity of the LC and, as a result, to the limitations in the predictability of the system. The focus of this study is to further elucidate the evolution of the LC, by quantifying the influence of coherent eddy fluxes originating in the Caribbean Sea. This is achieved by employing the Lagrangian-Averaged Vorticity Deviation (LAVD) method, an objective metric to evaluate eddy coherence in the Caribbean Sea that allows, for the first time, to quantify at different depths the evolution of coherent Caribbean eddies through the Yucatan Channel towards the GoM. The physical connectivity between the Caribbean Sea and the GoM is addressed using Lagrangian techniques to analyze processes that take place south of the Yucatan Channel and help quantify their strong relationship with the GoM eddy field. Coherent anticyclonic vorticity fluxes, as well as the net coherent anticyclonic volume transport between the Caribbean Sea and the GoM are associated with Loop Current Eddy (LCE) detachments through direct connectivity between the coherent Caribbean anticyclones and the forming LCE. The findings have important implications for understanding and predicting the LC system and the physical connectivity processes between the GoM and the Caribbean Sea.
Effects of water deficit at different stages on growth and ear quality of waxy maize

Introduction Extreme weather has occurred more frequently in recent decades, which results in more frequent drought disasters in the maize growing season. Severe drought often decreases remarkably plant growth and yield of maize, and even reduces significantly the quality of maize production, especially for waxy maize. Results To study the changes in plant growth, fresh ear yield, and fresh grain quality of waxy maize under water deficits occurring at different growth stages, and further strengthen the field water management of waxy maize, water deficit experiments were carried out under a rain shelter in 2019 and 2020. Water deficit treatments were imposed respectively at the V6–VT (DV6–VT), VT–R2 (DVT–R2), and R2–R3 (DR2–R3) stages of waxy maize, and treatment with non-water deficit in the whole growing season was taken as the control (CK). The lower limit of soil water content was 50% of field capacity for a water deficit period and 65% of field capacity for a non-water deficit period. Results In this study, water deficits imposed at V6–VT and VT–R2 stages decreased plant growth rate and leaf gas exchange parameters, accelerated leaf senescence, and limited ear growth of waxy maize, which resulted in 11.6% and 23.1% decreases in grains per ear, 19.4% and 7.3% declines in 100-grain weight, 20.3% and 14.2% losses in fresh ear yield in 2019 and 2020 growing seasons, respectively, while water deficit at R2–R3 stage had no significant effect on ear traits and fresh ear yield, but the fresh ear yield with husk of DR2–R3 decreased by 9.1% (P<0.05). The obvious water deficit imposed at the V6–VT and VT–R2 stages also lowered grain quality. Water deficits at the V6–VT and VT–R2 stages led to accelerated maturity, resulting in increased total protein, starch, and lysine content in grains at the R3 stage and decreased soluble sugar content. Principal component analysis revealed that when water deficits occurred in the waxy maize growing season, they firstly altered maize physiological processes, then affected ear characteristics and yield, and finally resulted in significant grain quality changes. In conclusion, a water deficit during V6–VT and VT–R2 not only reduced fresh ear yield but also adversely affected grain quality. However, water deficit during R2–R3 had little effect on total protein, starch, and soluble sugar content,but increased obviously lysine content. Discussion The above results suggested that avoiding serious water deficits at the V6–VT and VT–R2 stages of waxy maize while imposing a slight water deficit at the R2–R3 stage has not only little effects on fresh ear yield but also a remarkable improvement in grain quality.
Numerical study on the impact of water-rock interactions on the propagation of water-flooding induced fracture

Introcution: Water-flooding is an effective way to develop the extra-low permeability oil reservoirs and the water-flooding induced fracture (WIF) can improve waterflooding efficiency. However, geomechanical properties of rocks may alter due to the water-rock interactions, which usually increases the heterogeneity of reservoir properties and affects the WIF propagation. Methods: In this study, a method to calculate the WIF propagation length was proposed and numerical models were established considering the effect of water-rock interactions on geomechanical properties. In addition, the numerical model of stress distribution of the WIF propagation was validated by theoretical solutions based on the fracture mechanics. Moreover, the WIF propagation mechanism and the effect of water-rock interactions on the WIF propagation was analyzed. Results: Results indicate that the numerical model is valid to calculate the stress distribution induced by the WIF with an error under 5%. In addition, the WIF propagation can be affected by several factors, including the injection pressure, the damage variable and the initial fracture length. Noteworthy, the injection pressure should be strictly controlled because the WIF propagation is highly sensitive to the injection pressure. An increase of 2 MPa in the injection pressure can result in 90 m WIF propagation length, accounting for 64% of initial fracture length in the case studied in this work. Discussion: Moreover, water-rock interactions aggravate the heterogeneity of the reservoir and promote the WIF propagation due to the alteration of the geomechanical properties such as Young’s modulus. Therefore, the WIF can propagate even if the injection pressure is less than the minimum horizontal stress. Furthermore, the stress intensity factor (K IA) and WIF propagation length increase with the initial fracture length, which may lead to sudden water flux and reduce the efficiency of waterflooding.