Miracle-dz/UniFAR-FTUs huggingface dataset:
ROS generated from biotic stress: Effects on plants and alleviation by endophytic microbes

Aerobic living is thought to generate reactive oxygen species (ROS), which are an inevitable chemical component. They are produced exclusively in cellular compartments in aerobic metabolism involving significant energy transfer and are regarded as by-products. ROS have a significant role in plant response to pathogenic stress, but the pattern varies between necrotrophs and biotrophs. A fine-tuned systemic induction system is involved in ROS-mediated disease development in plants. In regulated concentrations, ROS act as a signaling molecule and activate different pathways to suppress the pathogens. However, an excess of these ROS is deleterious to the plant system. Along with altering cell structure, ROS cause a variety of physiological reactions in plants that lower plant yield. ROS also degrade proteins, enzymes, nucleic acids, and other substances. Plants have their own mechanisms to overcome excess ROS and maintain homeostasis. Microbes, especially endophytes, have been reported to maintain ROS homeostasis in both biotic and abiotic stresses by multiple mechanisms. Endophytes themselves produce antioxidant compounds and also induce host plant machinery to supplement ROS scavenging. The structured reviews on how endophytes play a role in ROS homeostasis under biotic stress were very meager, so an attempt was made to compile the recent developments in ROS homeostasis using endophytes. This review deals with ROS production, mechanisms involved in ROS signaling, host plant mechanisms in alleviating oxidative stress, and the roles of endophytes in maintaining ROS homeostasis under biotic stress.
Generation and characterization of a novel gne Knockout Model in Zebrafish

GNE Myopathy is a rare, recessively inherited neuromuscular worldwide disorder, caused by a spectrum of bi-allelic mutations in the human GNE gene. GNE encodes a bi-functional enzyme responsible for the rate-limiting step of sialic acid biosynthesis pathway. However, the process in which GNE mutations lead to the development of a muscle pathology is not clear yet. Cellular and mouse models for GNE Myopathy established to date have not been informative. Further, additional GNE functions in muscle have been hypothesized. In these studies, we aimed to investigate gne functions using zebrafish genetic and transgenic models, and characterized them using macroscopic, microscopic, and molecular approaches. We first established transgenic zebrafish lineages expressing the human GNE cDNA carrying the M743T mutation, driven by the zebrafish gne promoter. These fish developed entirely normally. Then, we generated a gne knocked-out (KO) fish using the CRISPR/Cas9 methodology. These fish died 8–10 days post-fertilization (dpf), but a phenotype appeared less than 24 h before death and included progressive body axis curving, deflation of the swim bladder and decreasing movement and heart rate. However, muscle histology uncovered severe defects, already at 5 dpf, with compromised fiber organization. Sialic acid supplementation did not rescue the larvae from this phenotype nor prolonged their lifespan. To have deeper insights into the potential functions of gne in zebrafish, RNA sequencing was performed at 3 time points (3, 5, and 7 dpf). Genotype clustering was progressive, with only 5 genes differentially expressed in gne KO compared to gne WT siblings at 3 dpf. Enrichment analyses of the primary processes affected by the lack of gne also at 5 and 7 dpf point to the involvement of cell cycle and DNA damage/repair processes in the gne KO zebrafish. Thus, we have established a gne KO zebrafish lineage and obtained new insights into gne functions. This is the only model where GNE can be related to clear muscle defects, thus the only animal model relevant to GNE Myopathy to date. Further elucidation of gne precise mechanism-of-action in these processes could be relevant to GNE Myopathy and allow the identification of novel therapeutic targets.
A positive association between hunger in childhood and frailty in old age: Findings from the Chinese longitudinal healthy longevity survey

Background Childhood hunger not only directly affects the physical and mental health of children and adolescents but also has a long-term negative effect on later health outcomes. In this cross-sectional study, we used a nationally representative Chinese sample to examine the relationship between hunger in childhood and frailty in older adults. Materials and methods The data were obtained from the 2018 Chinese Longitudinal Healthy Longevity Survey. The frailty index with 44 health deficits was used to identify frailty. Childhood hunger was measured by the question “Did you often go to bed hungry as a child? ” Insurance status was categorized as New Rural Cooperative Medical Scheme (NRCMS), Urban Basic Medical Insurance Scheme (UBMIS), others, and no insurance. Multivariate logistic regression analysis was performed to estimate the adjusted relationship between childhood hunger and frailty. Results A total of 7,342 older people aged 65 years and older were analyzed in this study. Older people who experienced childhood hunger were more likely to have frailty than those who did not (OR = 1.13, 95% CI: 1.02–1.26), after adjustment for sociodemographic characteristics, family/social support, socioeconomic status, insurance status, and health behaviors. The association of childhood hunger with frailty was found in the 65–79 years group (OR = 1.21, 95% CI: 1.03–1.43), women (OR = 1.25, 95% CI: 1.08–1.45), individuals with rural residence (OR = 1.16, 95% CI: 1.03–1.31), agricultural work (OR = 1.16, 95% CI: 1.00–1.34), financial dependence (OR = 1.18, 95% CI: 1.02–1.37), and those participating in NRCMS (OR = 1.35, 95% CI: 1.16–1.56). Participants with hunger in childhood who were 80 years or older (OR = 0.80, 95% CI: 0.65–0.98) had lower odds of frailty. NRCMS (OR = 1.42, 95% CI: 1.02–1.98) showed increased odds of childhood hunger-related frailty. Conclusion Exposure to hunger during childhood is linked to frailty among older adults, and age, financial support, and insurance status may mediate this relationship. Targeted interventions and policies to address frailty in older adults should be implemented.
UX Tri: Period Change Discovered and Unchanged Strong Blazhko Effect Confirmed

New CCD observations of the RRab star UX Tri after JD = 2452234 show a change of the main pulsation period. The period is now P = 0.4669046 +/- 0.0000006 [d]. The change is delta_P = - 1.72 x 10-5 [d] if compared with the value we previously published in IBVS 5210. A strong Blazhko effect with unchanged Blazhko period P_B = 43.7 +/- 0.1 [d] is confirmed by this paper. Light curve particularities around Blazhko phase phi_B = 0.0 are discussed in detail. In one observation (JD 2453617) related to Blazhko phase phi_B = 0.12 an exceptionally pronounced "bump" of delta_m > -0.1 magnitude was observed at pulsation phase phi = 0.71.
Endoscopic treatment for calcific tendinitis of the gluteus medius: A case report and review

Tendon calcification is a common disease, with the shoulder rotator cuff being the most common site. However, calcific tendinitis of the gluteus medius has rarely been reported. This study reports the case of a 64-year-old woman diagnosed with calcific tendinitis of the gluteus medius and experiencing right lateral hip pain with no apparent trigger. After unsuccessful conservative treatment, hip endoscopy was performed on this patient, allowing for a clear view of a “toothpaste-like” lesion in the gluteus medius tendon. A shaver was used to remove the lesion. After 8 weeks and 14 months of follow-up, the patient could return to regular daily and social activities. This study shows that endoscopic surgery can lead to effective, rapid recovery and minimally invasive clinical outcomes in patients with tendon calcification near the hip joint.
Clinical predictors and RT-PCR profile of prolonged viral shedding in patients with SARS-CoV-2 Omicron variant in Shanghai: A retrospective observational study

Objective To evaluate determinants of prolonged viral RNA shedding in hospitalized patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant infection. Materials and methods Hospitalized patients tested SARS-CoV-2 positive by nasopharyngeal real-time reverse transcriptase-polymerase chain reaction (RT-PCR) were included in the single-center, retrospective study. Patients were divided into 2 groups according to the timing of viral clearance (≤ 8 days, “early clearance” and ≥15 days, “late clearance”). Results 4,084 patients were included in the study (1,023 late clearance, 3,061 early clearance), with median age of 50 years and a higher proportion (61.4%) of male. Univariate analyses showed that comorbidities (including hypertension, diabetes, and coronary heart disease), receiving vaccine, the number of vaccinations, cycle threshold (Ct) open reading frame 1ab (ORF 1ab), and nucleocapsid protein (N) gene values on admission were associated with late viral clearance. In the multivariable analysis, the number of vaccinations (P = 0.010) and Ct ORF 1ab gene (P < 0.001) values on admission were significantly associated with late viral clearance. Generalized Estimating Equations (GEE) analysis showed that the Ct value of ORF 1ab gene and N gene remained unchanged within 3 days, and showed progressively higher values with increasing days during late viral RNA clearance. Conclusion The number of vaccinations and Ct values of ORF 1ab gene were independently associated with a prolonged SARS-CoV-2 RNA shedding.
A VIEW OF THE NARROW-LINE REGION IN THE INFRARED: ACTIVE GALACTIC NUCLEI WITH RESOLVED FINE-STRUCTURE LINES IN THE SPITZER ARCHIVE

We queried the Spitzer archive for high-resolution observations with the Infrared Spectrograph of optically selected active galactic nuclei (AGNs) for the purpose of identifying sources with resolved fine-structure lines that would enable studies of the narrow-line region (NLR) at mid-infrared wavelengths. By combining 298 Spitzer spectra with 6 Infrared Space Observatory spectra, we present kinematic information of the NLR for 81, z ≲ 0.3 AGNs. We used the [Ne v], [O iv], [Ne iii], and [S iv] lines, whose fluxes correlate well with each other, to probe gas photoionized by the AGN. We found that the widths of the lines are, on average, increasing with the ionization potential of the species that emit them. No correlation of the line width with the critical density of the corresponding transition was found. The velocity dispersion of the gas, σ, is systematically higher than that of the stars, σ*, in the AGN host galaxy, and it scales with the mass of the central black hole, MBH. Further correlations between the line widths and luminosities L, and between L and MBH, are suggestive of a three-dimensional plane connecting to a linear combination of log (σ) and log (L). Such a plane can be understood within the context of gas motions that are driven by AGN feedback mechanisms, or virialized gas motions with a power-law dependence of the NLR radius on the AGN luminosity. The MBH estimates obtained for 35 type 2 AGNs from this plane are consistent with those obtained from the relation.
Requirements for two-source entanglement concentration

Abstract Entanglement enhancement is a key task for quantum technologies. This operation performed on states produced by parametric down-conversion sources has been the object of several recent experimental investigations. In particular, conditional preparation by photon-subtraction has been shown to improve the entanglement of these states. Here we analyse the role played by non-Gaussian and Gaussian measurements in more general entanglement concentration operations performed on a pair of two-mode squeezed vacua. We find stringent requirements for achieving an improved entanglement enhancement by measuring jointly these two resource states.
Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

Escherichia coli is considered an opportunistic pathogen and an indicator for antimicrobial resistance (AMR) monitoring. Despite many reports on its AMR monitoring, studies based on genome-based analysis of AMR genes are still insufficient. Here, 181 E. coli strains were isolated from anal swab samples collected from pigs and chickens of animal farms located in Eastern China and sequenced through the Illumina platform. The results showed that 87.85% (159/181) of the E. coli isolates were multidrug-resistant (MDR). Ampicillin (AMP)- spectinomycin (SPT)- tetracycline (TET)- florfenicol (FFC)- sulfisoxazole (SF)- trimethoprim/sulfamethoxazole (SXT) was the predominant AMR pattern. By whole-genome sequencing, we found that ST10 (10.49%, 19/181) and ST48 (7.18%, 13/181) were major sequence types. IncFIB and IncX1 were the most prevalent plasmid replicons. The AMR genes blaNDM-5 (1.10%, 2/181), mcr-1 (1.10%, 2/181), tet(X4) (1.10%, 2/181), and cfr (6.08%, 2/181) were also found in these isolates. In addition, among the 169 virulence genes detected, we identified astA (37.02%, 67/181), hlyA (1.66%, 3/181), hlyB (1.66%, 3/181) and hlyD (1.66%, 3/181), which were closely related to heat-stable enterotoxin 1 and α-hemolysin. In addition, there were 33 virulence genes associated with the iron uptake system, and 46 were adhesion-related genes. Our study highlighted the need for routine surveillance of AMR with advanced genomic approaches, providing up-to-date data on the prevalence of AMR for the development and execution of antimicrobial stewardship policy.
Human amniotic membrane application in oral surgery—An ex vivo pilot study

Objectives: The purpose of this pilot porcine study was to explore and illustrate the surgical application of human amniotic membrane (hAM) in an ex vivo model of medication-related osteonecrosis of the jaw (MRONJ). Material and methods: Five oral and maxillofacial surgeons participated to this study. MRONJ was simulated on porcine mandible specimens. hAM was applied using four different techniques: implantation with complete coverage, implantation with partial coverage, apposition and covering graft material. At the same time, the surgeons evaluated how well the hAM handled and its physical properties during the surgery. Results: Surgeons found that hAM had suitable mechanical properties, as it was easy to detach from the support, handle, bind to the defect and bury. hAM was also found to be strong and stable. The “implantation with complete coverage” and “implantation with partial coverage” techniques were the preferred choices for the MRONJ indication. Conclusion: This study shows that hAM is a graft material with suitable properties for oral surgery. It is preferable to use it buried under the gingiva with sutures above it, which increases its stability. This technical note aims to educate surgeons and provide them with details about the handling of hAM in oral surgery. Clinical relevance: Two surgical techniques for hAM application in MRONJ were identified and illustrated. hAM handling and physical properties during surgery were reported.
Alternative zebra-structure models in solar radio emission.

In the literature, discussion continues about the nature of the zebra structure (ZS) in type IV radio bursts, and understanding even the most extended mechanism associated with double plasma resonance has been improved in series of works. Moreover, in the recent work (Ben\'a\v{c}hek, Karlick\'y, Yasnov, 2017) its ineffectiveness was shown under the usually adopted conditions in the radio source. In this case in a number of works we demonstrated the possibility of modeling with whistlers to explain many thin components of ZS stripes, taking into account the effects of scattering whistlers on fast particles. This situation stimulates the search for new mechanisms. For example, earlier we showed the importance of explosive instability, at least for large flares with the ejections of protons. In the system a weakly relativistic beam of protons, nonisothermic plasma, the slow beam mode of the space charge possesses negative energy, and in the triplet slow and fast beam modes and ion acoustic wave an explosive cascade of harmonics from ionic sound is excited. Electromagnetic waves in the form of ZS stripes appear as a result of the fast protons scattering on these harmonics. Such a mechanism can also be promising for a ZS in radio emission from the pulsar in the Crab nebula.
MicroRNAs in Kawasaki disease: An update on diagnosis, therapy and monitoring

Kawasaki disease (KD) is an acute autoimmune vascular disease featured with a long stage of febrile. It predominantly afflicts children under 5 years old and causes an increased risk of cardiovascular combinations. The onset and progression of KD are impacted by many aspects, including genetic susceptibility, infection, and immunity. In recent years, many studies revealed that miRNAs, a novel class of small non-coding RNAs, may play an indispensable role in the development of KD via differential expression and participation in the central pathogenesis of KD comprise of the modulation of immunity, inflammatory response and vascular dysregulation. Although specific diagnose criteria remains unclear up to date, accumulating clinical evidence indicated that miRNAs, as small molecules, could serve as potential diagnostic biomarkers and exhibit extraordinary specificity and sensitivity. Besides, miRNAs have gained attention in affecting therapies for Kawasaki disease and providing new insights into personalized treatment. Through consanguineous coordination with classical therapies, miRNAs could overcome the inevitable drug-resistance and poor prognosis problem in a novel point of view. In this review, we systematically reviewed the existing literature and summarized those findings to analyze the latest mechanism to explore the role of miRNAs in the treatment of KD from basic and clinical aspects retrospectively. Our discussion helps to better understand the pathogenesis of KD and may offer profound inspiration on KD diagnosis, treatment, and prognosis.
TM6SF2 rs58542926 is related to hepatic steatosis, fibrosis and serum lipids both in adults and children: A meta-analysis

Background and aims Findings about the associations between transmembrane 6 superfamily member 2 (TM6SF2) rs58542926 and nonalcoholic fatty liver disease have not been consistently replicated, particularly in steatosis and fibrosis. The present study aimed to investigate the associations between the rs58542926T allele and the spectrum of NAFLD and its related metabolic phenotypes. Methods Systematic literature research was performed to analyse the associations between rs58542926 and the spectrum of NAFLD and its related metabolic phenotypes. A random effects meta-analysis with a dominant genetic model was applied. Results Data from 123,800 individuals across 44 studies were included in the current meta-analysis.rs58542926 T allele was associated with an increased risk of NAFLD in both adults (OR=1.62; 95% CI: 1.40, 1.86) and children (OR=2.87; 95% CI: 1.85, 4.46). Children had a stronger association with NAFLD (P=0.01). rs58542926 T allele was also positively associated with steatosis progression (mean difference=0.22; 95% CI: 0.05, 0.39) and fibrosis stage (OR=1.50; 95% CI: 1.20, 1.88) in adults. The TM6SF2 rs58542926 T allele was positively associated with ALT in both adults and children (both P<0.01) and only with higher AST in adults (P<0.01). The rs58542926 T allele was negatively associated with serum total cholesterol (TC), low-density lipoprotein (LDL), and triglycerides (TGs) in both adults and children (all P<0.01). The serum level of TG was much lower in adults than in children (P<0.01). Conclusion TM6SF2 rs58542926 is involved in the entire spectrum of NAFLD and its related metabolic phenotype, and differences in serum lipid levels were observed between adults and children. Systematic review registration https://www.crd. york.ac. uk/PROSPERO/, identifier CRD42021288163.
Open Research Online Comparing welfare regimes by their eﬀects on intra-household inequalities

[A head] Abstract Gender analyses of policies tend to evaluate their effects on gender equality in access to the labour market and on gender roles within households; these have been examined both within and across different welfare state regimes (see for example Lewis 2009). However relatively little attention has been paid to effects on gender inequalities in access to and control over households’ financial resources. This chapter analyses the effect of employment status, to capture individuals’ paid and unpaid contributions to their household, on changes in satisfaction with household income, taken as an indicator of relative access to household resources. Intra-household gender inequalities can arise through policies’ effects both on the intra-household division of contributions and on the salience of that division to men’s and women’s access to household resources. Results are compared for three countries, Australia, Germany and the UK, chosen, not only because of the characteristics of their welfare regimes, but also because they all collect good household panel data that can be used for empirical investigation of such intra-household effects, and some comparative results are presented in this chapter.
S1366728922000128jra 890..898

The present study investigated the foreign language effect within an altruistic decision making process. Chinese–English bilinguals made altruistic decisions in their native (L1: Chinese) and second language (L2: English). The decisions were framed in two ways: either as “not to harm” (harm frame) or as “to help” the other person (help frame) at one’s economic cost. Behavioral results suggest that bilinguals might behave more altruistically in the harm frame than the help frame (i.e., framing effect) in their native language but not in their foreign language. Electrophysiological results show that the modulation of the framing effect in the native versus foreign language originated in the early ERP components (N1 and N2) and did not present in the late positive potential (LPP). These findings suggest the foreign language effect most likely results from the reduced emotional reaction in a foreign compared to the native language.
Impact of Real Agricultural Outputs, Human Capital and Power Supply on Nigeria’s Real Economic Growth (1981-2020)

- For over three decades (1981-2020), the Nigerian government has set out various policies to stimulate the country’s real economic growth. The government did this by inducing funds in different sectors: agriculture, education, health and power. Contrastingly, the desired outcome of real economic growth has not been achieved. Hence, this study examined the impact of real agricultural outputs, human capital and power supply on Nigeria's real economic growth using quarterly time-series data from 1981-to 2020; and exploited the Cobb-Douglas Production for theoretical backings. After reviewing relevant works of literature, the analytical techniques (Johansen cointegration (long-run relationship) and Dynamic Ordinary Least Squares (DOLS) (long-run impacts) were found worthy and were employed. The result of Johansen cointegration indicated a long-run equilibrium relationship in the model, while the Dynamic OLS result revealed that real agricultural output has been the key sector contributing the highest level of impact among other variables of interest to Nigeria’s real economic growth. Divergently, public spending on health and electricity has not significantly contributed to Nigeria's real economic growth. Thus, we conclude that real agricultural output has yielded a positive and significant impact on the nation’s growth, whereas spending on human health and electricity