Miracle-dz/UniFAR-FTUs huggingface dataset:
Microsphere-Encapsulated Antigen In Vivo -glycolic) Acid co Cross-Present Poly(lactic-Dendritic Cells and Macrophages - CD8

The analysis of cell types involved in cross-priming of particulate Ag is essential to understand and improve immunotherapies using microparticles. In this study, we show that murine splenic dendritic cells (DCs) as well as macrophages (M F s) are able to efﬁciently endocytose poly(lactic- co -glycolic) acid (PLGA) microspheres (MS) and to cross-present encapsulated Ags in the context of MHC class I molecules in vitro. A comparison of puriﬁed CD8 + and CD8 2 DCs indicated that both DC subtypes are able to present OVA-derived epitopes on MHC class I and II in vitro. To determine the contribution of DCs and M F s to cross-priming of PLGA MS in vivo, DCs were depleted in transgenic CD11c-DTR mice, and M F s were depleted by clodronate liposomes in wild-type mice before immunizing mice with OVA-encapsulated MS. Our results show that the depletion of DCs or M F s alone only led to minor differences in the OVA-speciﬁc immune responses. However, simultaneous depletion of DCs and M F s caused a strong reduction of primed effector cells, indicating a redundancy of both cell populations for the priming of PLGA MS-encapsulated Ag. Finally, we analyzed PLGA MS trafﬁcking to draining lymph nodes after s.c. injection. It was evident that ﬂuorescent particles accumulated within draining lymph nodes over time. Further analysis of PLGA MS-positive lymphatic cells revealed that mainly CD8 2 DCs and M
Organic Matter and Mineral Composition of Silicate Soils: FTIR Comparison Study by Photoacoustic, Diffuse Reﬂectance, and Attenuated Total Reﬂection Modalities

: This study aims to compare photoacoustic (FTIR–PAS), diffuse reﬂectance (DRIFT), and attenuated total reﬂection (ATR) FTIR modalities in the wide wavenumber range from NIR (7500 cm − 1 ) to FIR (150 cm − 1 ) for the same silicate soil samples under the same conditions. The possibilities of non-destructive rapid qualitative analysis of soils by these modalities without comprehensive data treatment were compared. The assignment of more than 100 bands for the chernozem and sod-podzolic as common types of silicate types of soil was made. The following groups of bands of organic matter and inorganic matrix were reliably found in spectra of all or at least two modalities: 3690–3680 cm − 1 (hydrogen-bonded SiO–H . . . H 2 O stretch, not ATR), 2930–2910 cm − 1 and 2860–2850 cm − 1 (methylene stretch), 1390–1380 cm − 1 , (symmetric stretch carboxylate, DRIFT and FTIR–PAS); 2000–1990 cm − 1 , 1885 cm − 1 , and 1790–1783 cm − 1 (SiO 2 overtones, DRIFT and FTIR– PAS), 1163–1153 cm − 1 , SiO 2 lattice (not FTIR–PAS), 1037 cm − 1 (Si–O or Al–O stretch), 796 cm − 1 (lattice symmetrical Si–O–Si stretch); 697 cm − 1 , SiO 2 ; and 256 cm − 1 (not FTIR–PAS). Amide I, II, and III bands appear in DRIFT and FTIR–PAS spectra while not in ATR. Except for methylene and carboxylate groups, CH vibrations (3100–2900 cm − 1 ) are not seen in ATR. Bands at 1640–1630 cm − 1 , 1620–1610 cm − 1 , 1600–1598 cm − 1 (primary water bands and probably carboxylate) appear in the spectra of all three modalities but are unresolved and require data treatment. It is preferable to use all three modalities to characterize both soil organic matter and mineral composition. DRIFT provides the maximum number of bands in all three modalities and should be selected as a primary technique in the NIR and 4000–2000 cm − 1 regions for hydrogen-bonding bands, CH X groups, and the silicate matrix. ATR–FTIR complements DRIFT and provides a good sensitivity for soil water and the matrix in 2000–400 cm − 1 . FTIR–PAS in 4000–1500 cm − 1 reveals more bands than DRIFT and shows the highest sensitivity for absorption bands that do not appear in DRIFT or ATR-IR spectra. Thus, FTIR–PAS is expedient for supporting either DRIFT or ATR–FTIR. This modality comparison can be a basis for methodological support of IR spectroscopy of soils and similar organomineral complexes.
Continued fractions for some algebraic numbers.

where <*! = 2 cos — =is a root of x -\x — 2x — l, #2 is a root of x — χ — l, OCB = j/2~+ j/3"is a root of X — 9z — 4# + 27z — 36z — 23. The first numbers were chosen s cubic irrationalities, oc1 s being totally real, and we picked a2, OCB at random to be non cubic. The original motivation was to see if computations for algebraic numbers would be in line with some conjectures made in [3] and [4], and to observe whatever eise might come up.
Influence of Gender Equality Initiatives on Performance of Female Journalists in the Media Industry in Kenya

: Gender mainstreaming has variously been found to affect the performance of employees in organisations. Studies conducted in the past have established that gender mainstreaming is an important aspect of performance in the workplace. One important aspect of gender mainstreaming is gender equality initiative in the workplace. This study sought to investigate the influence of gender equality initiative on performance of female journalists in Kenya in terms of promotions, assignments, flexibility of working arrangements and the composition of employees. The results of this study will assist organizational managers to implement initiatives and programs that will enhance gender equality thus improving the performance of female employees in the workplace. The cross-sectional study which was conducted in four mainstream media organizations in Kenya used self-administered questionnaires which were used to collect data from randomly selected 320 male and female journalists working in the Standard Media Group, Nation Media Group, Kenya Broadcasting Corporation (KBC) and Media Max Limited. The survey established that gender equality initiative influences female journalist’s performance to a statistically significant extent. Additionally, it was found that gender equality initiative accounts for a 24.6 percent variation in the performance of female journalists. It is therefore important to ensure that organizational management take cognizance of how gender equality initiative can actually influence the performance of female employees within their organisations .
Final Results in Directional Links Analysis , and Algorithm Designs

This deliverable brings the final results on directional links' analysis and algorithm design. The results, models, and analysis herein append the results given in the previous deliverable (D2.1) of Task 2.1 of the ARIADNE project. This deliverable reports the models and analysis for practical hardware imperfections, reconfigurable intelligent surface (RIS) gain properties, RIS beamforming algorithms, and performance analysis of RIS-aided links in this deliverable. Horizon 2020 – 871464 – ARIADNE Deliverable D2.3
PLATELETS AND THROMBOPOIESIS CEACAM2 negatively regulates hemi (ITAM-bearing) GPVI and CLEC-2 pathways and thrombus growth in vitro and in vivo

glycoprotein expressed on blood, epithelial, and vascular cells. CEACAM2 possesses adhesive and signaling properties mediated by immunoreceptor tyrosine-based inhibitory motifs. In this study, we demonstrate that CEACAM2 is expressed on the surface and in intracellular pools of platelets. Functional studies of platelets from Ceacam2 2 / 2 deficient mice ( Cc2 2 / 2 ) revealed that CEACAM2 serves to negatively regulate collagen glycoproteinVI(platelet)(GPVI)-FcR g –chainandtheC-typelectinlikereceptor2(CLEC-2) signaling. Cc2 2 / 2 platelets displayed enhanced GPVI and CLEC-2–selective ligands, collagen-related peptide (CRP), collagen, and rhodocytin (Rhod)-mediated platelet aggregation. They also exhibited increased adhesion on type I collagen, and hyperresponsive CRP and CLEC-2–induced a and dense granule release compared with wild-type platelets. Furthermore, using intravital microscopytoferricchloride(FeCl 3 )–injuredmesentericarteriolesandlaser-inducedinjuryofcremastermusclearterioles,weherein show that thrombi formed in Cc2 2 / 2 mice were larger and more stable than wild-type controls in vivo. Thus, CEACAM2 is a novel platelet immunoreceptor that acts as a negative regulator of platelet GPVI-collagen interactions and of ITAM receptor CLEC-2 pathways. ( Blood . 2014;124(15):2431-2441)
A study of simulated reionization histories with merger trees of HII regions

Aims. We describe a new methodology for analyzing the reionization process in numerical simulations. The chronology and the geometry of reionization is investigated by the merger histories of individual HII regions. Methods. From the merger tree of ionized patches, one can track the individual evolution of the regions properties, such as their size, the intensity of the percolation process by looking at the formation rate, the frequency of mergers, and the number of individual HII regions involved in the mergers. We applied the merger-tree technique to simulations of reionization with three di erent kinds of ionizing source models and two resolutions. Two of them use star particles as ionizing sources. In this case we compared two emissivity evolutions for the sources to reach the reionization at z 6. As an alternative we built a semi-analytical model where the dark matter halos extracted from the density fields are assumed as ionizing sources. Results. We then show how this methodology is a good candidate to quantify the impact of the adopted star formation on the history of the observed reionization. The semi-analytical model shows a homogeneous reionization history with local hierarchical growth steps for individual HII regions. On the other hand, autoconsistent models for star formation tend to present fewer regions, with a dominant region in size that governs the fusion process early in the reionization at the expense of the local reionizations. The di erences are attenuated when the resolution of the simulation is increased.
University of Birmingham Healthcare governance, professions and populism: is there a relationship?

A new wave of support for populist parties and movements represents a serious threat to universal healthcare coverage in traditional liberal democracies and beyond. This article aims to contribute empirical material on the relationships between healthcare governance, professions and populism. It applies an explanatory cross-country comparative approach and uses mixed methods, including micro-level data garnered from international comparative databases and documents. Denmark, England, Germany, Italy and Turkey have been selected for comparison, reflecting different types of healthcare systems and populist movements. The results reveal variety in the ways populist discourses impact in healthcare. Abundant economic resources, network-based governance, high levels of trust in healthcare providers and doctors participating as insiders in the policy process seem to work as a bulwark against populist attacks on healthcare and professional expertise. On the other hand, poorly resourced NHS systems with doctors as outsiders in the policy process and major NPM reforms together with low to medium levels of trust in healthcare providers may be fertile ground for populist discourse to flourish. Our explanatory data provide hints of correlations, which may inform further studies to investigate causality. Yet the research highlights that healthcare governance and professions matter, and brings into view capacity for counteracting populist attacks on universal healthcare and professional knowledge.
Parental Inﬂuence on Children’s Computational Thinking in an Informal Setting (Fundamental Research)

Informal learning environments such as science centers and museums are instrumental in the promotion of science, technology, engineering, and mathematics (STEM) education. These settings provide children with the chance to engage in self-directed activities that can create lifelong interest and persistence in STEM. In addition, the participation of parents in these settings can engage children in conversations that can boost understanding and enhance learning of STEM topics. To date, a considerable amount of research has focused on adult-child dialogue. Findings from those studies revealed that children experience more elaborate scientific thinking when parents facilitate learning. Given the need for engineers to have computer science skills, academic discourse has placed emphasis on studying computational thinking (CT) in children. While some recent studies have focused on the roles parents play to promote children’s engineering thinking, very few studies have explored parents’ influence on children’s engagement in CT. Therefore, in this study, we investigate the roles that parents play in promoting computational thinking in their young children.
Spotlight on Asteroid Hyalosis: A Clinical Perspective

: Asteroid hyalosis is a benign degenerative condition of the vitreous that is often asymptomatic. Our purpose with this narrative review is to provide a current overview of asteroid hyalosis from a clinical perspective. We review the epidemiology and risk factors, the clinical diagnosis and differential diagnoses, clinical challenges in eyes with asteroid hyalosis, and the treatment of asteroid hyalosis. Many clinical questions remain unanswered, and the current evidence to guide clinical practice is largely based on anecdotal evidence and clinical experience. More studies on asteroid hyalosis from a clinical perspective are warranted.
SUMMARY OF CLINICOPATHOLOGIC DATA Biopsy Site Biopsy Site Case

We describe the clinical, histologic, immunophenotypic, and genotypic features of five cases of histologically discordant lymphomas with B-cell and T-cell components. Three patients presented with B-cell lymphoma; T-cell lymphoma subse quently developed. One patient presented with T-cell lym phoma; B-cell lymphoma subsequently developed. One patient presented with synchronous B-cell and T-cell lymphomas. There were three men and two women. The median age at the initial diagnosis of lymphoma was 66 years. The mean interval between the development of the two lymphomas was 83 months. All patients died of disease. The mean survival was 96 months after the initial diagnosis of lymphoma and 14 months The term discordance, or discordant lymphoma, is used to describe the phenomenon of that involve separate the term of the immunophenotype. 1-3 Immunophenotypic, cytogenetic, and genotypic studies have shown that the components usually repre sent different morphologic expressions of the same neo plastic clone. 1-5 Although histologically distinct, they have been shown to express identical monoclonal after the diagnosis of the histologically discordant lymphoma. Epstein-Barr virus was found in two cases—the B-cell lym phoma in the patient who presented with synchronous lym phomas, and the subsequent T-cell lymphoma in one of the patients who presented with B-cell lymphoma. Based on the results of immunophenotypic and genotypic analyses, these cases likely represent the occurrence of two distinct lymphoid neoplasms rather than histologic progression of the same neo plastic clone. Furthermore, a subset of these cases are Epstein-Barr virus-associated.
Work in Progress: Openness, Conscientiousness, Self-Direction, and Mindset in First-Year Engineering Students

Work in Progress: Openness, Conscientiousness, Self-Direction, and Mindset in First-Year Engineering Students Dr. Matthew Cavalli, Western Michigan University Dr. Cavalli is Professor of Mechanical and Aerospace Engineering and Associate Dean for Undergraduate Academic Affairs in the College of Engineering and Applied Sciences. Ms. Anetra Grice, Western Michigan University Anetra Grice has served as the STEM Talent Expansion Program Director for Western Michigan University’s College of Engineering and Applied Sciences for since 2010.
NOTES Contemporary Prevalence of BRO (cid:2) -Lactamases in Moraxella catarrhalis : Report from the SENTRY Antimicrobial Surveillance Program (North America, 1997 to 2004)

A total of 7,860 community-acquired Moraxella catarrhalis isolates (SENTRY Antimicrobial Surveillance Program, 1997 to 2004) were tested by broth microdilution methods, and 399 randomly selected strains from North American sites were tested for BRO-1 and BRO-2 by PCR methods. Several antimicrobials remained very active, including amoxicillin-clavulanate (MIC 90 s, < 0.25 (cid:1) g/ml), azithromycin (MIC 90 s, < 0.12 (cid:1) g/ml), ceftriaxone (MIC 90 s, 0.5 (cid:1) g/ml), and levoﬂoxacin (MIC 90 s, < 0.03 to 0.06 (cid:1) g/ml). The BRO-2 incidence rates by year were 3 to 4% overall (96 to 97% for BRO-1) and were the highest in Canada (7.9%), with the incidence in the United States being only 2.0%.
The increase of the pulmonary blood flow inhigh-risk hypoxic patients with a bidirectional Glenn anastomosis

Background: An additional shunt in single ventricle patients with Glenn anastomosis may increase pulmonary flow at the expense of ventricle volume overloading. The performance of the modification depends on pulmonary resistance, indicating better results in favorable hemodynamic conditions. Aims: The study aims at analyzing the influence of precisely adjusted pulsatile shunt in borderline high-risk Glenn patients on early and late results. Methods: The study involved 99 patients (including 21 children) with the bidirectional Glenn and accessory pulsatile shunt (BDGS group), and 78 patients with the classic bidirectional Glenn anastomosis (BDG group). Results: There was 1 death in the BDGS group and 4 deaths in the BDG group. No difference in mortality ( P = 0.71) was found. The Fontan completion was achieved in 69 (88.5%) children in the BDG group and 18 (85.7%) patients in the BDGS group, without fatalities. No intergroup differences in postoperative pulmonary artery pressure ( P = 0.10), ventilation time ( P = 0.12), the McGoon ratio ( P = 0.9), or chylothorax frequency ( P = 0.14) were observed. Intensive care unit ( P = 0.28) and hospitalization ( P = 0.05) times were comparable. Echocardiography revealed no significant differences in the ventricle and atrioventricular valve function between groups. In the BDGS group, higher blood oxygen saturation ( P = 0.03) and increase of the McGoon index ( P = 0.002) were noted. Conclusions: Bidirectional Glenn anastomosis with precisely adjusted accessory pulmonary blood flow provides stable hemodynamics and adequate oxygen saturation in borderline, profoundly hypoxic patients. An advantageous pulmonary artery development before Fontan completion was observed. 638–644 The study presents the results of assisted Glenn anastomosis in high-risk single ventricle patients. An additional pulsatile pulmonary blood flow source was precisely constructed and adjusted intraoperatively in patients in whom otherwise it was impossible to win from by-pass. The results show that bidirectional Glenn anastomosis with an accessory pulsatile blood flow source can assure safe hemodynamics and satisfactory development of the pulmonary arteries as a preparation for Fontan completion. This option may be considered as a solution in borderline Fontan candidates or definitive palliation instead of Fontan completion.
1 2 3 Non-invasive assessment of carotid arterial wave speed and distensibility 4 5 6

Northumbria University has developed Northumbria Research Link (NRL) to enable users to access the University’s research output. Copyright © and moral rights for items on NRL are retained by the individual author(s) and/or other copyright owners. Single copies of full items can be reproduced, displayed or performed, and given to third parties in any format or medium for personal research or study, educational, or not-for-profit purposes without prior permission or charge, provided the authors, title and full bibliographic details are given, as well as a hyperlink and/or URL to the original metadata page. The content must not be changed in any way. Full items must not be sold commercially in any format or medium without formal permission of the copyright holder. The full policy is available online: http://nrl.northumbria. ac.uk/pol i cies.html
17 (cid:1) -Estradiol, Aging, Inflammation, and the Stress Response in the Female Heart

Heat shock proteins (HSPs) are a cardioprotective class of proteins induced by stress and regulated by the transcription factor, heat shock factor (HSF)-1. 17 (cid:1) -estradiol (E 2 ) indirectly regulates HSP expression through rapid activation of nuclear factor- (cid:2) B (NF- (cid:2) B) and HSF-1 and protects against hypoxia. As males experience a loss of protective cellular responses in aging, we hypothesized that aged menopausal (old ovariectomized) rats would have an impaired HSP response, which could be prevented by immediate in vivo E 2 replacement. After measuring cardiac function in vivo , cardiac myocytes were isolated from ovariectomized adult and old rats with and without 9 weeks of E 2 replacement. Myocytes were treated with E 2 in vitro and analyzed for activation of NF- (cid:2) B, HSF-1, andHSPexpression.Inaddition,wemeasuredinflammatorycytokineexpressionandsusceptibility to hypoxia/reoxygenation injury. Cardiac contractility was reduced in old ovariectomized rats and could prevented by immediate E 2 replacement in vivo . Subsequent investigations in isolated cardiac myocytes found that in vitro E 2 activated NF- (cid:2) B, HSF-1, and increased HSP 72 expression in adultbutnotoldrats.Inresponsetohypoxia/reoxygenation,myocytesfromadult,butnotold,rats had increased HSP 72 expression. In addition, expression of the inflammatory cytokines TNF- (cid:3) and IL-1 (cid:1) , as well as oxidative stress, were increased in myocytes from old ovariectomized rats; only the change in cytokine expression could be attenuated by in vivo E 2 replacement. This study demon-strates that while aging in female rats led to a loss of the cardioprotective HSP response, E 2 retains its protective cellular properties. ( Endocrinology 152: 1589–1598, 2011) esterases DCFDA,
Glucose, memory, and aging 1–3

Circulating glucose concentrations regulate many brain functions, including learning and memory. Much of the evidence for this view comes from experiments assessing stress-related release of epinephrine with subsequent increases in blood glucose concentrations. One application of this work has been to investigate whether age-related memory impairments result from dysfunctions in the neuroendocrine regulation of the brain processes responsible for memory. Like humans, aged rodents exhibit some memory impairments that can be reversed by administration of epinephrine or glucose. In elderly humans, ingestion of glucose enhances some cognitive functions, with effects best documented thus far on tests of verbal contextual and noncontextual information. Glucose also effectively enhances cognition in persons with Alzheimer disease or Down syndrome. Although earlier evidence suggested that glucose does not enhance cognitive function in healthy young adults, more recent ﬁndings suggest that glucose is effective in this population, provided the tests are sufﬁciently difﬁcult. In college students, glucose consumption significantly enhanced memory of material in a paragraph. Glucose also appeared to enhance attentional processes in these students. Nei-ther face and word recognition nor working memory was inﬂu-enced by treatment with glucose. The neurobiological mechanisms by which glucose acts are under current investigation. Initial evidence suggests that glucose or a metabolite may activate release of the neurotransmitter acetylcholine in rats when they are engaged in learning. Consequently, the issue of nutrition and cognition becomes increasingly important in light of evidence that circulating glucose concentrations have substantial effects on brain and cognitive functions. Am J Clin Nutr 1998;67(suppl):