Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial and temporal variation in precipitation at the start of the rainy season in tropical Australia

The patterns of rainfall early in the rainy season vary substantially across northern Australia, even in sites with the same annual average. This has biophysical and economic implications in terms of land and infrastructure management, resource availability and capacity, and access. Daily patterns in long-term rainfall records in Australia north of 23°S subject to regular monsoonal rainfall were compared with threshold levels for dryland and wetland seed germination, initiation of the growing season, patterns of gaps between early storms and the heaviness of the first falls, correlations between thresholds, spatial variation in correlation with the Southern Oscillation Index (SOI) and temporal trends in mean threshold dates. The earliest rains sufficient to cause seed germination or generate fresh fodder occur in the north-west of the Northern Territory with the average date being later to the south, east and west. Initial falls of the rainy season are heaviest, however, on Cape York Peninsula so that the time between first falls and saturation is shortest in the east. The probability of extended gaps between rainfall events increased from north to south. When the SOI is taken into account, no change in timing could be detected at the few sites with records of sufficient duration. However, because of changes in SOI frequency, rains are tending to start earlier in the drier parts of the north and north-west and later in the east. This may be because anthropogenic climate change is resulting in fewer classical El Nino Southern Oscillation events and more frequent El Nino Modoki climate anomalies.
Kinetics of glucose stimulatory effect on silica deposition and growth of natural populations of Fragilaria crotonensis

Diatoms are known to exploit organic substrates for growth; however, convincing evidence that they can utilize dissolved organic carbon under natural conditions is not available. In 2008–2009, we performed in situ experiments examining the effect of glucose addition on silica deposition kinetics and growth rates of Fragilaria crotonensis in the eutrophic Římov Reservoir (Czech Republic). Silica deposition kinetics was measured at 4‐h intervals over a 24‐h incubation with PDMPO [2‐(4‐pyridyl)‐5{[4‐dimethylaminoethyl‐aminocarbamyl)‐methoxy] phenyl}oxazole] fluorescence probe. A significant stimulatory effect of glucose supplemented at the concentration of 10−4 M on Fragilaria silification was observed at 20 and 24 h. Fragilaria growth rates almost doubled upon glucose enrichment compared with the untreated control at 24 h. In addition, we conducted a dose‐response experiment testing the glucose additions from 10−8 to 10−3 M in a 24‐h incubation. Glucose stimulated both Fragilaria silification and growth at concentrations >10–7 M, which might occasionally occur in a reservoir as a result of accidental contamination of water by organic pollution.
Spatial distribution of spawning sites of pikeperch [Sander lucioperca (L.)] in a highly eutrophic clay‐turbid lake – implications for management

Summary The spawning sites of pikeperch were mapped using artificial spawning nests in the clay-turbid Lake Tuusulanjarvi in southern Finland to protect the most important spawning areas. Altogether 272 artificial spawning nests were set before actual spawning on potential spawning grounds. The first observations of eggs were made on 26 May at a surface water temperature of 12°C. No new eggs were found after 10 June at water temperature of 16°C. The nests were removed on 18 June when the larvae had already hatched. The study demonstrated that artificial spawning nests could be used to evaluate both duration and spatial dimensions of spawning of pikeperch.
Effects of variable Shading in a Greenhouse Study on rhizome Weight, Root Length, and bud Proliferation in Goldenseal

Goldenseal (Hydrastis canadensis) is an herbaceous perennial valued for its fleshy rhizomes, which contain the alkaloids hydrastine and berberine. While it is understood that relative shade influences growth and yield of goldenseal, optimal shade level for maximum rhizome mass and plant vigor under cultivation has not been established. Goldenseal plants grown from cold stratified rhizomes were kept under shade for 5 months in the greenhouse. Treatments were five different shade levels ranging from 60% to 95% of full shade, plus a control group in full sun. Measured variables included rootlet length, bud development, and rhizome mass. Plants grown under moderate shade (60 to 70%) produced longer and more numerous rootlets, more bud primordia, and had greater rhizome mass and healthier leaves than plants grown under extreme shade (95%) or in full sun. Decreasing shade density had a major impact on plant condition and growth. Those plants grown with the greatest sun exposure displayed 100% scorch damage to the foliage, in comparison to <35% damage in the moderate shade (30 to 40%). The results suggest that moderate shading may double yield in rhizome mass, and promote increased bud proliferation in subsequent seasons. Late season leaf vigor is not correlated with rhizome mass.
The Coevolution of Pine Squirrels (Tamiasciurus) and Conifers

Field observations of discriminatory feeding behavior of squirrels indicate that in the process of maximizing their own feeding efficiency squirrels act as selective agents on several characteristics of plant reproduction. Calorimetric measurements of the energy content of plant reproductive organs confirm the evolutionary effects of discriminatory feeding by squirrels. The reproductive characteristics of conifers which are affected include several details of cone anatomy, the number of seeds per cone, the time at which cones shed their seeds, the thickness of seed coats, and annual fluctuations in the size of cone crops. A prolonged evolutionary interaction between rodent—like, arboreal mammals and the reproductive organs of the modern genera in the family Pinaceae is consistent with the fossil record. A general analysis of selective pressure influencing terrestrial plant reproduction indicates that seed eaters could have a marked influence on the evolution of plant reproduction. Among the interrelated s.
Advances in the captive breeding and reproductive biology of the short-beaked echidna (Tachyglossus aculeatus)

Abstract. Captive breeding of the short-beaked echidna (Tachyglossus aculeatus) has proven a difficult challenge; as recently as 2009, there were fewer than 10 echidnas born in captivity. We present observations of captive reproductive behaviour following video surveillance and measurements of body temperature collected from six captive female echidnas over a six-year period. In the first series of observations (2009–10) we examined the efficacy of artificial burrow boxes as possible aids for reproductive success. Females with access to burrow boxes had significantly higher levels of reproductive activity (P = 0.001), there was coincidental improvement in the production of eggs or pouch young (two eggs, one unhatched and one offspring). During 2009–10, a range of reproductive behaviours (courtship, copulation and postcopulation) were documented and analysed, as were new observations of oestrous cycle activity. Female body temperature was characteristically stable during egg incubation during this study and has the potential to be used as a tool for the assessment of reproductive status. Following initial observations, burrow boxes and infrared lamps were implemented as standard husbandry in our echidna breeding facility and the effects on reproductive success were monitored, albeit less intensively, for a further four years (2011–14). Although no direct causal effect could be ascribed, the use of burrow boxes and heat lamps coincided with a total of 13 young being born to four females in the last four years (2011–14). These female echidnas were found to be receptive at intervals throughout the breeding season, both before and after presumed incubation phases, suggesting that captive animals exhibit polyoestry. In 2012 and 2014, the same female showed evidence of producing two young from one breeding event.
Sexual biology of Pandemis pyrusana (Lepidoptera: Tortricidae) under laboratory conditions

Laboratory studies were conducted to characterize some aspects of the sexual biology of Pandemis pyrusana Kearfott. Both males and females were sexually active during their first scotophase. Virgin females held at 22°C started calling the first night 4 - 5 hrs into scotophase. Calling by virgin females occurred earlier and continued longer into scotophase after the first night. Mating lasted 3 - 4 hrs and both sexes mated only once per evening. Calling frequency by mated females was lower than for virgins and dropped off sharply after 2 nights. Forty percent of females mated more than once during the 6-day test. Males mated on consecutive scotophases, but the percentage of subsequent copulations passing a spennatophore declined with age. Oviposition occurred throughout a diurnal cycle, but was concentrated during early scotophase. Females laid an average of four egg masses from which 219 larvae eclosed. Egg mass size, number of larvae emerging, and the number of larvae emerging per egg mass area declined with subsequent egg masses. Key words: Pandemis; leafrollers; apple; sexual behaviour
NMDA receptor subunits GluRε1, GluRε3 and GluRζ1 are enriched at the mossy fibre–granule cell synapse in the adult mouse cerebellum

Cerebellar N‐methyl‐d‐aspartate (NMDA) receptors are concentrated in the granular layer and are involved in motor coordination and the induction of long‐term potentiation at mossy fibre–granule cell synapses. In the present study, we used immunohistochemistry to examine the distribution of NMDA receptor subunits in the adult mouse cerebellum. We found that appropriate pepsin pretreatment of sections greatly enhanced the sensitivity and specificity of immunohistochemical detection. As a result, intense immunolabelling for GluRε1 (NR2A), GluRε3 (NR2C), and GluRζ1 (NR1) all appeared in synaptic glomeruli of the granular layer. Double immunofluorescence showed that these subunits were colocalized in individual synaptic glomeruli. Within the glomerulus, NMDA receptor subunits were located between centrally‐located huge mossy fibre terminals and peripherally‐located tiny Golgi axon terminals. By immunoelectron microscopy, all three subunits were detected at the postsynaptic junction in granule cell dendrites, forming synapses with mossy fibre terminals. Consistent with the known functional localization, GluRε1, GluRε3, and GluRζ1 are, thus, anatomically concentrated at the mossy fibre–granule cell synapse. By contrast, immunohistochemical signals were very low in Purkinje cell somata and dendrites in the molecular layer. The lack of GluRζ1 immunolabelling in Purkinje cells was unexpected because the cells express GluRζ1 mRNA at high levels and high levels of GluRζ1 protein in the molecular layer were revealed by immunoblot. As Purkinje cells are exceptionally lacking GluRε expression, the discrepant result may provide in vivo evidence suggesting the importance of accompanying GluRε subunits in synaptic localization of GluRζ1.
TESTING FOR LATITUDINAL BIAS IN DIVERSIFICATION RATES: AN EXAMPLE USING NEW WORLD BIRDS

Study of the latitudinal diversity gradient to date has focused largely on pattern description, with relatively little work on the possible mechanisms underlying the pattern. One proximate mechanism is a latitudinal bias in the discrepancy between speciation and extinction rates, leading to higher rates of species diversification toward lower latitudes. Despite being central to many explanations for high tropical diversity, this mechanism is tested very rarely. We discuss some of the problems involved in testing for latitudinal bias in diversification rates and present an example phylogenetic analysis for endemic bird genera of the New World. The results provide evidence for higher diversification rates in clades inhabiting lower latitudes, both when genera are considered independent and when phy- logeny is controlled for using independent contrasts. High rates of diversification are also associated with larger geographic area and higher net primary productivity, although these do not fully account for the latitudinal effect. The latitudinal pattern is stronger in younger clades, supporting the prediction of a simple model in which the signal of latitudinal bias in diversification rates diminishes as clades age and become saturated with species. Our study demonstrates that a clade-based approach can help answer important questions that a geographic approach cannot, but large phylogenies and geographic databases are needed to cope with the large amount of noise inherent in this type of analysis.
Camouflaging of seeds treated with pesticides mitigates the mortality of wild birds in wheat and rice crops

Seeds used to plant wheat, corn and rice crops in Brazil are treated with the insecticide carbofuran associated with the dye rhodamine B, attracting granivorous birds and causing mortality during sowing. The objective of this study was to evaluate if using camouflaged seeds can minimize mortality caused by the ingestion of seeds with carbofuran. Alternatives for reducing mortality, such as using carbofuran without rhodamine B or replacing carbofuran by carbosulfan, were compared. Three experiments were carried out in regions of known bird mortality in the states of Parana and Sao Paulo, Brazil, using conventional sowing over plowed soil in the traditional planting system, 15,896 kg of seeds in 111.46 ha. Bird carcasses were collected and dissected to unveil which poisoned seed treatments had caused birds' deaths. The mortality mounted to 296 birds of 11 species. Eared doves were the most numerous casualties (263). There was significant correlation between mortality and number of consumed seeds. The consumption of camouflaged seeds was lower than that of commercial seeds treated with rhodamine B. The mortality caused by seeds with both rhodamine B and carbofuran was higher than mortality caused by seeds camouflaged only with carbofuran. The replacement of carbofuran with carbosulfan also seemingly reduced mortality, but carbosulfan mortality might have been underestimated as a result of the apparent movement of affected birds after exposure. Seeds treated with carbofuran and rhodamine B and without any dye, were attractive. Because legal requirement for seeds treated with pesticides to be differentiated by dying, the substitution of rhodamine B by camouflaging must be encouraged.
Top‐down control of rare species abundances by native ungulates in a grassland restoration

Ecological restorations are predicted to increase in species diversity over time until they reach reference levels. However, chronosequence studies in grasslands often show that diversity peaks after the first few years and then declines over time as grasses become more dominant. We addressed whether bison grazing and seed additions could prevent this decline in diversity. Exclosures that prevented bison grazing were compared with grazed plots over 4 years, and seed additions were conducted inside and outside exclosures to test for seed and microsite limitations. A previous study conducted 4‐months post seeding found that local species richness was primarily seed limited, but that grazing could sometimes increase seedling emergence. Here, we tested whether increased seedling emergence led to longer‐term increases in the species diversity of the plant community. We found that the seed addition effect grew smaller and the grazing effect grew stronger over time, and that seed additions affected the abundance of added species only when plots were grazed. Grazed plots had higher species diversity and lower biomass and litter buildup compared to non‐grazed plots. Our results suggest that moderate grazing by bison or management that mimics grazing can maintain diversity in grass‐dominated situations. Our results also emphasize the need to follow seed additions over several years to assess correctly whether seed limitation exists.
Herbage mass and nutritive value of herbage of extensively managed temperate grasslands along a gradient of shrub encroachment

Semi-natural grasslands often serve as important reserves of biodiversity. In Europe extensive grazing by livestock is considered an appropriate management to conserve biodiversity value and to limit shrub encroachment. However, little is known about the influence of shrubs on agronomic values. A gradient analysis of shrub-invaded temperate grasslands (from shrub-free to pioneer forest) in Germany was carried out to test the hypothesis that herbage mass and variables describing nutritive value of herbage decrease with increasing shrub encroachment. The herbage mass of dry matter (DM), variables describing the nutritive value of herbage, composition of the vegetation and mean of Ellenberg’s indicator values were analysed with respect to the extent of shrubs. There was a reduction of herbage mass of DM from 3570 to 210 kg ha−1 with increasing shrub encroachment. Metabolizable energy concentration of herbage ranged from 8·9 to 10·2 MJ kg−1 DM and crude protein concentration from 72 to 171 g kg−1 DM, both measures being positively correlated with shrub occurrence. Increasing shrub occurrence was associated with a decrease in water-soluble carbohydrates concentration (from 151 to 31 g kg−1) and a reduction in the indicator ‘forage value’. The results indicate a potentially large agronomic value for shrub-encroached temperate grasslands.
The impact of Bt maize as a natal host on adult head capsule width in field populations of western corn rootworm

The success of the current resistance management plan for transgenic maize, Zea mays L. (Poaceae), targeting the rootworm complex hinges upon high rates of mating between resistant and susceptible beetles. However, differences in the fitness of adult beetles could result in assortative mating, which could, in turn, change the rate of resistance evolution. Adult head capsule widths of naturally occurring populations of western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), were examined in a variety of refuge configurations. Beetles were classified into treatments based on the hybrid – non‐Bt refuge or Bt maize targeting larval rootworms (hereafter Bt‐RW maize) – and location – proximity to other Bt‐RW or refuge plants – of the natal host plant. Treatments included the following: a refuge plant surrounded by other refuge plants, a refuge plant located near a Bt‐RW plant, a Bt‐RW plant surrounded by Bt‐RW plants, and a Bt‐RW plant located near a refuge plant. The mean head capsule width of males emerging from Bt‐RW plants was significantly smaller than the mean head capsule width of males emerging from refuge plants. These results indicate that males emerging from Bt‐RW maize plants may be exposed to sublethal doses of the Bt toxin as larvae. No differences were detected between females emerging from refuge plants compared with Bt‐RW plants. Overall mean head capsule width decreased as the season progressed, regardless of treatment. The diminished head capsule width of western corn rootworm males emerging from Bt‐RW maize may act to enhance resistance management, particularly in a seed mix refuge system.
Comparative health risk surveillance of heavy metals via dietary foodstuff consumption in different land-use types of Pakistan

ABSTRACT This study probes heavy metals (HMs) concentration in groundwater, soil, vegetables, chicken eggs, and buffalo milk samples collected from different land-use types (LUT) with special emphasis on human health risk via their consumption. Our results depicted that HMs (Ni, Cr, Pb, and Cd) concentration in groundwater of all LUT; Cd concentration in agricultural soil; Ni, Cr, Mn, Cd, and Pb concentration in buffalo milk; and Ni, Cd concentration in chicken eggs of all LUT surpassed the recommended permissible limits. While, on the other hand, Cr concentration in industrial and Pb concentration in agricultural LUT also exceeded permissible limits in the case of chicken egg samples. The concentration of Cr, Pb, and Cd in most of the vegetable samples of different LUT also crossed permissible limits. The accumulation factor for selected HMs followed trends for different LUT as Industrial > Agricultural > Residential, showing the transfer of risk from soil to vegetables. Our results for principle component analysis unravel that, unlike residential, industrial, and agricultural, LUT were highly affected from metals contaminations when different environmental matrices were studied. Health risk index (HRI) was chronicled >1 for Cd in groundwater of industrial and residential sites, in the eggs found in the industrial site, and for Pb in groundwater of industrial and agricultural sites due to higher daily intake of metal, while all other HMs revealed HRI < 1 in all LUT.
Allozyme variation and population structure of Carex humilis var. nana (Cyperaceae) in Korea

Genetic diversity and population structure of 22 Carex humilis var. nana Ohwi (Cyperaceae) populations in Korea were determined using genetic variation at 23 allozyme loci. This is a long-lived herbaceous species with a widespread distribution in eastern Asia. The 12 enzymes revealed 23 putative loci, of which 11 were polymorphic (47.8%). Genetic diversity at the varietal level and at the population level was 0.131 and 0.118, respectively. Total genetic diversity (HT = 0.274) and within population genetic diversity (HS = 0.256) were high, whereas the extent of the population divergence was relatively low (GST = 0.068). An indirect estimate of the number of migrants per generation (Nm = 3.42) indicated that gene flow was high among Korean populations. Wide geographic ranges, perennial herbaceous nature, and the persistence of multiple generations are associated with the high level of genetic variation. A distinct difference between Asian and North American Carex is shown in the proportion of genetic variat.
Rates of straying by hatchery-produced Pacific salmon (Oncorhynchus spp.) and steelhead (Oncorhynchus mykiss) differ among species, life history types, and populations

Here we ask whether straying differs among species, life history types, and populations of adult hatchery-produced Pacific salmon (Oncorhynchus spp.) and steelhead (Oncorhynchus mykiss) in the Columbia River basin. Previous estimates of straying have been confounded by various factors influencing the probability of individuals returning to non-natal sites (e.g., off-station releases), whereas analyses undertaken here of nearly a quarter million coded-wire tag recoveries control for these factors. Our results revealed large and generally consistent differences in the propensity to stray among species, life history types within species, and populations. Paired releases indicated that (i) Chinook salmon (Oncorhynchus tshawytscha) strayed more (mean population range 0.11%–34.6%) than coho salmon (Oncorhynchus kisutch) (0.08%–0.94%); (ii) ocean-type Chinook (5.2%–18.6%) strayed more than stream-type Chinook (0.11%–10%); and Chinook salmon (0.90%–54.9%) strayed more than steelhead (0.30%–2.3%). We conclude thes.