Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic Variation of the Mitochondrial ND4 Region among Geographical Populations of Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) in China

Abstract A 327 bp region of the mitochondrial DNA NADH dehydrogenase 4 subunit (ND4) was analyzed for genetic variation in 43 wheat midges (Sitodiplosis mosellana) from 12 different areas of China. Nucleotide sequencing revealed 21 polymorphic sites and 22 distinct haplotypes. Haplotypes was closely related with geographical distribution based on Neighbor-Joining analysis. The haplotypes from the spring wheat region samples clustered into one group, and the majority of winter wheat region samples clustered into another group. However, the haplotypes in the Chang'an population in Shaanxi Province (SC) appeared synchronously in each group. Thus, the distribution area of the SC population became a transition place of haplotype distribution. The genetic distances among geographic populations of the wheat midges were 0.0000∼0.0180 and those within each populations were 0.0000∼0.0145.
Systems for Assessing the Effectiveness of Management in Protected Areas

Abstract Since the mid-1990s, numerous methodologies have been developed to assess the management effectiveness of protected areas, many tailored to particular regions or habitats. Recognizing the need for a generic approach, the World Commission on Protected Areas (WCPA) developed an evaluation framework allowing specific evaluation methodologies to be designed within a consistent overall approach. Twenty-seven assessment methodologies were analyzed in relation to this framework. Two types of data were identified: quantitative data derived from monitoring and qualitative data derived from scoring by managers and stakeholders. The distinction between methodologies based on data types reflects different approaches to assessing management. Few methodologies assess all the WCPA framework elements. More useful information for adaptive management will come from addressing all six elements. The framework can be used to adapt existing methodologies or to design new, more comprehensive methodologies for evaluation, using quantitative monitoring data, qualitative scoring data, or a combination of both.
Cemeteries - A Potential Risk to Groundwater

Cemeteries are a source of environmental impacts among which is featured the risk to groundwater contamination by microorganisms that proliferate during the process of decomposition of bodies. The main objective of this work was to monitor the bacteriological quality of the water from the water table in three cemeteries chosen in accordance with geological and hydrogeological criteria: Vila Formosa and Vila Nova Cachoeirinha, located in the city of Sao Paulo, and Areia Branca in the city of Santos. These cities are situated in the state of Sao Paulo, Brazil. A total of 67 samples were collected through 36 piezometers installed in the internal area of the above-mentioned cemeteries, and were analysed between January and December of 1989. For this study microorganisms of fecal contamination were chosen, one pathogen, as well as two groups degradation of organic material bacteria.
Antibiotic Resistance and Virulence Gene Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Healthy Edible Marine Fish

Thirty-three (33) isolates of methicillin-resistant Staphylococcus aureus (MRSA) from healthy edible marine fish harvested from two aquaculture settings and the Kariega estuary, South Africa, were characterised in this study. The phenotypic antimicrobial susceptibility profiles to 13 antibiotics were determined, and their antibiotic resistance determinants were assessed. A multiplex PCR was used to determine the epidemiological groups based on the type of SCCmec carriage followed by the detection of staphylococcal enterotoxin-encoding genes sea-sed and the Panton Valentine leucocidin gene (pvl). A high antibiotic resistance percentage (67–81%) was observed for Erythromycin, Ampicillin, Rifampicin, and Clindamycin, while maximum susceptibility to Chloramphenicol (100%), Imipenem (100%), and Ciprofloxacin (94%) was recorded. Nineteen (58%) of the MRSA strains had Vancomycin MICs of ≤2 μg/mL, 4 (12%) with MICs ranging from 4–8 μg/mL, and 10 (30%) with values ≥16 μg/mL. Overall, 27 (82%) isolates were multidrug-resistant (MDR) with Erythromycin-Ampicillin-Rifampicin-Clindamycin (E-AMP-RIP-CD) found to be the dominant antibiotic-resistance phenotype observed in 4 isolates. Resistance genes such as tetM, tetA, ermB, blaZ, and femA were detected in two or more resistant strains. A total of 19 (58%) MRSA strains possessed SCCmec types I, II, or III elements, characteristic of healthcare-associated MRSA (HA-MRSA), while 10 (30%) isolates displayed SCCmec type IVc, characteristic of community-associated MRSA (CA-MRSA). Six (18%) of the multidrug-resistant strains of MRSA were enterotoxigenic, harbouring the see, sea, or sec genes. A prevalence of 18% (6/33) was also recorded for the luk-PVL gene. The findings of this study showed that marine fish contained MDR-MRSA strains that harbour SCCmec types, characteristic of either HA-MRSA or CA-MRSA, but with a low prevalence of enterotoxin and pvl genes. Thus, there is a need for continuous monitoring and implementation of better control strategies within the food chain to minimise contamination of fish with MDR-MRSA and the ultimate spread of the bug.
Fruit yield and quality of Palmer mango trees under different irrigation systems

Mango production has been having a great economical expression in the Brazilian agriculture. However, the economic crisis and increased competitiveness of the international market required improvements in the crop efficiency for its sustainability. In this context, the objective of this work was to evaluate the effect of different irrigation system on the physiology and fruit yield and quality of Palmer mango trees in the semiarid conditions of the Lower Middle Sao Francisco Valley, Brazil. The experiment was carried out at the Agranvil Farm, in Petrolina, state of Pernambuco, Brazil, with harvests in July 2013 and October 2014. A randomized block statistical design was used, with four treatments replicated in 5 blocks, during two production cycles. The treatments consisted of four irrigation systems: one micro sprinkler under the plant canopy (MSPC); drip system with two lateral lines per plant row (DSLL); one micro sprinkler between plants (MSBP); and ring-shaped drip system around the plants (RSDS). All treatments provided a flow rate of 56 L h-1 plant-1. The physiological characteristics evaluated were: photosynthesis, stomatal conductance, leaf transpiration, and leaf temperature. Fruit yield, quantity, mean weight, volume, soluble solid content, titratable acidity (TA), density, and pulp firmness were evaluated after the harvest. The evaluated treatments presented significant differences for the physiological variables. The treatment DSLL provided higher number of fruits and fruit yield, and the treatment MSBP provided the lowest results for these variables.
Identification of Suitable Internal Control Genes for Quantitative Real-Time PCR Expression Analyses in Peanut (Arachis hypogaea)

Abstract Real-time qPCR is currently the most sensitive technique available for the detection of low-level mRNA expression. For more reliable and precise gene expression analyses, real-time PCR data for a sequence of interest must be normalized against that of a control gene, which is uniformly expressed in various tissues and during different phases of development. So far, suitable internal controls for gene expression studies in peanut have not been identified. We assessed the expression of 10 frequently used housekeeping genes, specifically ubq10, gapdh, hel1, yls8, 14-3-3, 60s, ubc, ef-1α, act7, and adh3. Using the algorithms available through the GeNorm and NormFinder programs, the stability of their expression was estimated in a set of five diverse peanut tissue samples derived from a Virginia-type peanut cultivar (Shulamit). Collectively, the gene with the most stable expression across all of the examined tissues and both programs was adh3, followed by 60s and yls8, which had minimal estimated intr.
Influence of wall-wetting conditions on in-flame and exhaust soot structures in a spark ignition direct injection petrol engine

Great attention to the efficiency benefits of spark ignition direct injection engine has been averted due to its problematic particulate emissions. In the present study, the fundamental knowledge of wall-wetting-induced spark ignition direct injection soot particles is enhanced through direct particle sampling from pool fire on the piston top surface and cylinder liner as well as from the exhaust stream. The sampled soot particles are imaged using transmission electron microscope, and the image post-processing for statistical morphology and internal structure analysis is performed to better understand the soot formation and oxidation processes. The experiments were performed in a single-cylinder optical spark ignition direct injection engine where diffusion flame luminosity was recorded using a high-speed camera through the cylinder liner window, with which the overall sooting level was understood, and the pool fire location was identified. Given the in-flame soot sampling experiments in the spark ignition direct injection engine were new, error analysis was conducted in terms of the number of fuel injections and engine run-to-run variations. This sampling technique then was applied for various injection timings in the intake stroke. The data analysis and physical interpretation was focused on a piston-wetting condition at the most advanced injection timing of 320 °CA bTDC and a liner-wetting condition at the most retarded injection timing of 180 °CA bTDC in the present study. Between these two different wall-wetting conditions, it was found that the piston-wetting condition has larger soot primary particles and soot aggregates. The internal carbon-layer fringe shows longer length, less tortuosity and smaller gap, indicating more mature and carbonised soot. This was consistent with more significant and wider distributed pool fire and thus longer soot residence time within the flames. When the exhaust soot particles were analysed, however, it was found that the reduction in soot aggregate size was much higher and the carbonisation was more progressed for the piston-wetting condition than those of the liner-wetting condition. This suggested higher soot oxidation later in the expansion/exhaust stroke for the piston-wetting condition, which potentially can be better utilised for engine applications.
Study of the thermal decomposition mechanism of FOX-7 by molecular dynamics simulation and online photoionization mass spectrometry

The thermal decomposition mechanism of energetic materials is important for analyzing the combustion mechanisms of propellants and evaluating the safety of propellants during transport and storage. 1,1-Diamino-2,2-dinitroethylene (FOX-7) is an important insensitive energetic material that can be used as an oxidizer in propellants. However, the initial decomposition mechanism of FOX-7 is not clear to date. The ReaxFF molecular dynamics method is widely used in the investigation of the thermal decomposition mechanisms of energetic materials. Meanwhile, the combination of thermogravimetry with online photoionization time-of-flight mass spectrometry (TG-PI-TOF-MS) and online single-photon ionization time-of-flight mass spectrometry (SPI-TOF-MS) can reveal the decomposition products, which may be integrated with the results of the simulation. In this study, the primary thermal decomposition mechanism of 1,1-diamino-2,2-dinitroethylene (FOX-7) was studied by the ReaxFF molecular dynamics simulations and online photoionization mass spectrometry. The results of the molecular dynamics simulations showed that the primary decomposition step of FOX-7 is C–NO2 cleavage; after this, C <svg xmlns="http://www.w3. org/2000/svg" version="1.0" width="13.200000pt" height="16.000000pt" viewBox="0 0 13.200000 16.000000" preserveAspectRatio="xMidYMid meet"><metadata> Created by potrace 1.16, written by Peter Selinger 2001-2019 </metadata><g transform="translate(1.000000,15.000000) scale(0.017500,-0.017500)" fill="currentColor" stroke="none"><path d="M0 440 l0 -40 320 0 320 0 0 40 0 40 -320 0 -320 0 0 -40z M0 280 l0 -40 320 0 320 0 0 40 0 40 -320 0 -320 0 0 -40z"/></g></svg> O formation occurs via a three-membered ring transition state, followed by NO elimination. The remaining structure loses NH2 and H, resulting in the formation of the NHCCO structure, which finally breaks down into HNC and CO. NH2 reacts with an H atom to produce NH3. A reversible intramolecular hydrogen transfer was also observed at 2500 K; however, it failed to dominate the decomposition reaction. During the decomposition of FOX-7, the major products are N2, NH3, CO2, and H2N2 and the minor products are H2O, HN2, and H2. The TG-PI-TOF-MS spectrum shows three signals, i.e., m/z = 18, 28, and 30, which can be assigned to H2O, CO, and NO, respectively. Moreover, four signals at m/z = 72.72, 55.81, 45.79, and 29.88 corresponding to the products (NH2)2CCO, (NH2)CCO, NO2, and NO have been obtained in the SPI-TOF-MS spectrum. The experimental data obtained via online photoionization mass spectrometry further validated the results of the molecular dynamics simulations.
Sexual reproduction of white spruce (Picea glauca)

Reproductive buds broke dormancy at the same time as vegetative buds. Pollen mother cells entered prophase of meiosis immediately after dormancy and five-celled, winged pollen was mature about 6 weeks later. Megasporogenesis occurred 3 weeks after microsporogenesis and the female gametophyte was mature in about 6 weeks. Pollination occurred over about 1 week in late May or early June and fertilization occurred about 3 weeks after pollination. One to four archegonia developed. A comparable number of 16-celled proembryos usually developed within 1 week after fertilization and cotyledons began to develop about 1 month after fertilization. Simple polyembryony occurred in most ovules but cleavage polyembryony was not observed. Embryos were fully developed in late August and seeds were mature and shed in September.The small number of archegonia often present, the high incidence of self-pollination, which may have been the cause of the high frequency of early embryo abortion, and the failure of basal and distal .
THE REGULATION OF NUMBERS OF TROPICAL OCEANIC BIRDS

SUMMARY The ways in which the numbers of tropical sea-birds might be limited are considered; it is argued that food is the only factor likely to be generally effective in limiting numbers, but it seems improbable that food shortage could exert a density-dependent control of the mortality of the birds outside the breeding season. Wynne-Edwards' hypothesis that colonies of sea-birds are able to keep their own numbers below the level at which food shortage would become acute, primarily by exerting control on the output of young, is rejected as unproven and improbable. It is suggested that colonies of tropical oceanic birds deplete the food in the waters round them, and that as the populations increase competition for food becomes more intense, and relatively fewer adults succeed in raising chicks. This would provide a density-dependent control of the output of young and could regulate the numbers of the birds. The peculiarities of long-lived sea-birds (e.g. clutches of one, long fledging periods, deferred maturity) which Wynne-Edwards suggests are adaptations evolved in order to lower the reproductive rate until it balances the mortality, apparently could not be evolved as such; they are more probably adaptations enabling the birds sometimes to raise single chicks in spite of competition for food that makes it impossible to raise more than one. It is considered that variation in the age of first breeding provides an important supplement to variation in reproductive success in regulating the numbers of long-lived tropical sea-birds. The possible applications of this hypothesis to sea-birds breeding in higher latitudes are briefly considered, as are its implications in relation to conservation and exploitation of populations of sea-birds.
Images of Roma through the Language of Bulgarian State Archives

This research has been carried out as part of the RomaInterbellum Project which studies the Roma civic emancipation between World War I and World War II. Trawling through the Bulgarian archival documents on Roma in this time period, a reader cannot help but begin to form a certain image about the Tsigani, the term with which Roma have been popularly referred to in the archives. Unsurprisingly, this image does not seem to differ much from the one of today—that of the uneducated, dirty, foreign, and that pose a threat not only to the prosperity and well-being of the Bulgarian population and culture at large but also to the state and the economy. The research is based on archived files, letters of complaints from Bulgarian citizens and other documents sourced from Bulgarian state archives. The article analyses the words and language employed in the archived documents, the connotations they bear and the images they build. It also tries to show how, in the interwar period, this dominant language was utilised by Roma individuals and leaders in order to react, counter and protect their image and future. More importantly, they sought ways to build a better integrated Roma society through the establishment of own organisations and associations. Understanding this historical narrative from the interwar period is essential in advancing knowledge of many major issues surrounding the Roma today, such as housing, health and their social inclusion.
EFFECTS OF CADMIUM ON HOUSEFLY: INFLUENCE ON GROWTH AND DEVELOPMENT AND METABOLISM DURING METAMORPHOSIS OF HOUSEFLY

Abstract The objective of this study was to elucidate the effects of cadmium on housefly, Musca domestica. It is suggested that of cadmium in low concentrations of had little influence on growth and development of housefly. Cadmium was mainly distributed in the digestive tracts of housefly larvae, where the cadmium content was higher than that of other parts. During the metamorphosis of housefly the change trend of the cadmium content was very apparent. The cadmium content increased gradually in larval period, whereas it decreased significantly after polarization. Until the sixth day after emergence, only negligible cadmium was left in adult housefly. All these results demonstrated that the response of housefly to cadmium is an evolution adaptation in natural selection.
Antibacterial Activity of the Leaves of Coccinia indica (W. and A) Wof India

2 Abstract: The aim of the present research was focused on investigating the antibacterial and preliminary phytochemical properties of Coccinia indica (W.A.) via in vitro approach. The aqueous and organic solvent (Petroleum ether, chloroform and ethanol) extracts from the leaves of Coccinia indica (Cucurbitaceae) were tested against Enterobacter aerogenes, Pseudomonas aeruginosa, Staphylococcus epidermidis, Bacillus subtilis and Salmonella typhimurium by agar well diffusion method and broth dilution method. Results showed promising antibacterial activity against the bacteria tested. Among these, ethanol and aqueous extracts were found to have a more potent inhibitory effect comparing with the other extracts. Which prove the potentiality of the plant extracts for the treatment of various skin and gastrointestinal infections in humans.
Characteristics of endemic-level mountain pine beetle populations in south-central Wyoming

This study was conducted to evaluate the dynamics of endemic populations of mountain pine beetle (Dendroctonus ponderosae Hopkins). In addition, we extended the geographical range of an existing data base recorded in Utah with similar data from Wyoming. This work was accomplished in lodgepole pine (Pinus contorta Dougl. Var. latifolia Engelm.) stands on the Medicine Bow National Forest in south-central Wyoming. Thirty-eight variable-radius paired plots (BAF 10) were measured during the summer of 1987. Host-tree condition and mountain pine beetle infestation characteristics were determined from currently and previously infested trees. Presence and severity of Armillaria root disease and stem pathogens was determined. Tree condition and infestation patterns were similar at this site to those found in earlier studies. Trees selected by endemic mountain pine beetle populations were infested with Comandra blister rust (Cronartium Comandra PK) and root disease (Armillaria spp.). Host-tree condition and mountain pine beetle infestation patterns recorded in this study parallel those identified earlier in Utah and will help land managers identify trees to cut to reduce stand hazard to mountain pine beetle infestation.
Inhibitory effects of brusatol delivered using glycosaminoglycan-placental chondroitin sulfate A-modified nanoparticles on the proliferation, migration and invasion of cancer cells

Breakthroughs in cancer management result from the development of drugs that can be used for early diagnosis and effective treatment. Surgery, chemotherapy, radiotherapy and hormone therapy are the main anticancer therapies. However, traditional cancer chemotherapy is associated with serious systemic side effects. Nanoparticles (NPs) provide an effective solution for cancer treatment via the targeted delivery of drugs to cancer cells, while minimizing injury to normal cells. Glycosaminoglycan-placental chondroitin sulfate A (plCSA) is expressed in a number of tumor cells and trophoblasts. A plCSA-binding peptide (plCSA-BP) was isolated from malaria protein VAR2CSA, which can effectively promote the binding of lipid polymer NPs to tumor cells, thereby significantly enhancing the anticancer effect of encapsulated drugs. Brusatol is an important compound derived from Brucea javanica that exerts a multitude of biological effects, including inhibiting tumor cell growth, reducing the reproduction of malaria parasites, reducing inflammation and resisting virus invasion. In the present study, brusatol-loaded NPs (BNPs) or coumarin 6 NPs (CNPs), plCSA-BP and scrambled control peptide-bound BNPs or CNPs were prepared. Ovarian cancer cells (SKOV3), endometrial cancer cells (HEC-1-A) and lung cancer cells (A549) were treated with the NPs. The uptake of plCSA-CNPs by tumor cells was found to be markedly higher compared with that of other types of NPs. Further studies demonstrated that the plCSA-BNPs promoted the apoptosis of cancer cells more effectively and inhibited their proliferation, invasion and migration, accompanied by downregulation of matrix metalloproteinase (MMP)-2, MMP-9 and B-cell CLL/lymphoma 2 (BCL2) levels, but upregulation of BCL2-associated X protein BAX and cleaved caspase-3 levels. The results demonstrated the potential of brusatol delivered by plCSA-modified NPs as a chemotherapeutic agent for the targeted therapy of tumors by regulating the BCL2, BAX, cleaved caspase-3, MMP-2 and MMP-9 pathways, and indicated that it may be an effective and safe strategy for the treatment of various tumors.