Miracle-dz/UniFAR-FTUs huggingface dataset:
Natural Progression of Routine Laboratory Markers after Spinal Trauma: A Longitudinal, Multi-Cohort Study

Our objective was to track and quantify the natural course of serological markers over the 1st year following spinal cord injury. For that purpose, data on serological markers, demographics, and injury characteristics were extracted from medical records of a clinical trial (Sygen) and an ongoing observational cohort study (Murnau study). The primary outcomes were concentration/levels/amount of commonly collected serological markers at multiple time points. Two-way analysis of variance (ANOVA) and mixed-effects regression techniques were used to account for the longitudinal data and adjust for potential confounders. Trajectories of serological markers contained in both data sources were compared using the slope of progression. Our results show that, at baseline (≤ 2 weeks post-injury), most serological markers were at pathological levels, but returned to normal values over the course of 6–12 months post-injury. The baseline levels and longitudinal trajectories were dependent on injury severity. More complete injuries were associated with more pathological values (e.g., hematocrit, ANOVA test; χ2 = 68.93, df = 3, adjusted p value <0.001, and χ2 = 73.80, df = 3, adjusted p value <0.001, in the Sygen and Murnau studies, respectively). Comparing the two databases revealed some differences in the serological markers, which are likely attributable to differences in study design, sample size, and standard of care. We conclude that because of trauma-induced physiological perturbations, serological markers undergo marked changes over the course of recovery, from initial pathological levels that normalize within a year. The findings from this study are important, as they provide a benchmark for clinical decision making and prospective clinical trials. All results can be interactively explored on the Haemosurveillance web site (https://jutzelec.shinyapps.io/Haemosurveillance/) and GitHub repository (https://github.com/jutzca/Systemic-effects-of-Spinal-Cord-Injury).
Complete, closed and curated genome sequences of Photobacterium damselae subsp. piscicida isolates from Australia indicate mobilome-driven localized evolution and novel pathogenicity determinants

Despite the recent advances in sequencing technologies, the complete assembly of multi-chromosome genomes of the Vibrionaceae , often containing several plasmids, remains challenging. Using a combination of Oxford Nanopore MinION long reads and short Illumina reads, we fully sequenced, closed and curated the genomes of two strains of a primary aquatic pathogen Photobacterium damselae subsp. piscicida isolated in Australia. These are also the first genome sequences of P. damselae subsp. piscicida isolated in Oceania and, to our knowledge, in the Southern hemisphere. We also investigated the phylogenetic relationships between Australian and overseas isolates, revealing that Australian P. damselae subsp. piscicida are more closely related to the Asian and American strains rather than to the European ones. We investigated the mobilome and present new evidence showing that a host specialization process and progressive adaptive evolution to fish are ongoing in P. damselae subsp. piscicida , and are largely mediated by transposable elements, predominantly in chromosome 2, and by plasmids. Finally, we identified two novel potential virulence determinants in P. damselae subsp. piscicida – a chorismate mutase gene, which is ubiquitously retained and co-localized with the AIP56 apoptogenic toxin-encoding gene on the pPHDP10 plasmid, and transfer-messenger RNA gene ssrA located on the main chromosome, homologous to a critical-to-virulence determinant in Yersinia pseudotuberculosis . Our study describes, to our knowledge, the only fully closed and manually curated genomes of P. damselae subsp. piscicida available to date, offering new insights into this important fish pathogen and its evolution.
Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts

Serological surveillance has become an important public health tool during the COVID-19 pandemic. Detection of protective antibodies and seroconversion after SARS-CoV-2 infection or vaccination can help guide patient care plans and public health policies. ABSTRACT The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this sensitive test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual’s immune response. The accuracy, adaptability, and cost-effectiveness of this test make it an excellent option for clinical deployment in the ongoing COVID-19 pandemic. IMPORTANCE Serological surveillance has become an important public health tool during the COVID-19 pandemic. Detection of protective antibodies and seroconversion after SARS-CoV-2 infection or vaccination can help guide patient care plans and public health policies. Serology tests can detect antibodies against past infections; consequently, they can help overcome the shortcomings of molecular tests, which can detect only active infections. This is important, especially when considering that many COVID-19 patients are asymptomatic. In this study, we describe an enzyme-linked immunosorbent assay (ELISA)-based qualitative and quantitative serology test developed to measure IgG and IgA antibodies against the SARS-CoV-2 spike glycoprotein. The test can be deployed using commonly available laboratory reagents and equipment and displays high specificity and sensitivity. Furthermore, we demonstrate that IgG titers in patient samples can be estimated from a single measurement, enabling the assay’s use in high-throughput clinical environments.
COVID-19 and physical activity in sedentary individuals: differences in metabolic, cardiovascular, and respiratory responses during aerobic exercise performed with and without a surgical face masks.

BACKGROUND The Coronavirus-19 (COVID-19) impairs metabolic, cardiovascular, and pulmonary functions in human metabolism, and wearing face masks is recommended for the prevention of contracting or exposing others to cardio-respiratory infections. Since the effect of wearing a surgical face mask (SFM) on cardiopulmonary exercise capacity has not been systematically reported we aimed to determine the effects of wearing SFM during an incremental walking test on metabolic, cardiovascular, and pulmonary gas exchange responses in sedentary individuals. METHODS The evaluations were performed using a repeated measures study design. Seven sedentary males (age:40years, height:178cm, weight:88kg, BMI:28kg/m2, VO2max:32.7±3.9ml/kg/min) and 7 sedentary female participants (age:34years, height:169cm, weight:62kg, BMI:22kg/m2, VO2max:32.1±6.8 ml/kg/min) volunteered to participate in the current study. Anthropometric parameters were measured using a Bioelectrical impedance analysis prior to each testing session. The measures of lung function assessed by spirometry, breathing pattern, maximal exercise capacity with-and-without mask were measured with a breath-by-breath automated exercise metabolic system during incremental Bruce protocol on a treadmill with two consecutive sessions with 48-h intervals. Blood pressure values (systolic and diastolic pressure) of the individuals were taken and recorded within 1 minute at the end of every ten minutes, without speed changes. RESULTS VO2, VCO2, and VE were significantly lower during exercise performed with SFM (p<0.001). Heart rate, systolic and diastolic blood pressure were also found significantly higher during exercise performed with SFM (p<0.01). CONCLUSIONS Wearing a SFM during incremental walking predispose a decrease in oxygen delivery while increasing pulmonary ventilation in sedentary individuals. Thus, it could be speculated that surgical face masks have a negative impact on oxygen delivery during exercise which results in decreased exercise performance due to the restricted ventilatory conditions.
Is birth out-of-hospital associated with mortality and morbidity by seven years of age?

Background and aims Compared to in-hospital births, the long-term outcome of children born out-of-hospital, planned or unplanned, is poorly studied. This study aimed to examine mortality and morbidity by seven years of age in children born out-of-hospital compared to those born in-hospital. Methods This study was registered retrospectively and included 790 136 children born in Finland between 1996 and 2013. The study population was divided into three groups according to birth site: in-hospital (n = 788 622), planned out-of-hospital (n = 176), and unplanned out-of-hospital (n = 1338). Data regarding deaths, hospital visits, reimbursement of medical expenses, and disability allowances was collected up to seven years of age or by the year-end of 2018. The association between birth site and childhood morbidity was determined using multivariable-adjusted Cox hazard regression analysis. Results No deaths were reported during the first seven years after birth in the children born out-of-hospital. The percentage of children with hospital visits due to infection by seven years of age was lower in those born planned out-of-hospital and in the combined planned out-of-hospital and unplanned out-of-hospital group compared to those born in-hospital. Furthermore, the percentage of children with hospital visits and who received disability allowances due to neurological or mental disorders was higher among those born unplanned out-of-hospital and out-of-hospital in total when compared to those born in-hospital. In the multivariable-adjusted Cox proportional hazard regression analysis, the hazard ratio for hospital visits due to asthma and/or allergic diseases (HR 0.84; 95% CI 0.72–0.98) was lower in children born out-of-hospital when compared to those born in-hospital. A similar decreased risk was found due to infections (HR 0.76; 95% CI 0.68–0.84). However, the risk for neurological or mental health disorders was similar between the children born in-hospital and out-of-hospital. Conclusions Morbidity related to asthma or allergic diseases and infections by seven years of age appeared to be lower in children born out-of-hospital. Birth out-of-hospital seemed to not be associated with increased risk for neurological morbidity nor early childhood mortality. Our study groups were small and heterogeneous and because of this the results need to be interpreted with caution.
Coronavirus anxiety and exhaustion among Polish front-line healthcare workers - the mediation effect of insomnia.

OBJECTIVES The study aimed to investigate the direct and indirect - mediated through insomnia - effect of coronavirus anxiety on exhaustion, from the perspective of Hobfoll's theory of conservation of resources (COR). According to the COR theory, critical events (e.g., the coronavirus epidemic) make people fearful of losing their valuable resources. A prolonged state of anxiety may lead to sleeping troubles, which over time results in an increase in exhaustion. MATERIAL AND METHODS Data were collected from 440 Polish healthcare providers, including nurses and midwives, doctors, paramedics, medical assistance workers, and wardens. Three measures were used: the Coronavirus Anxiety Scale, the Copenhagen Psychosocial Questionnaire (the sleeping trouble subscale) and the Oldenburg Burnout Inventory (the exhaustion subscale). Hypotheses were tested using structural equation modeling. RESULTS The obtained results fully support the hypotheses. Both the direct and indirect relationships between coronavirus anxiety and exhaustion were observed. Specifically, high coronavirus anxiety increased insomnia, which in turn contributed to the development of exhaustion. CONCLUSIONS The results are consistent with the COR theory. Prolonged coronavirus anxiety and sleeping problems depleted healthcare providers' resources and made them feel exhausted. Exhaustion among these workers can have serious consequences not only for themselves but also for the health of their patients. Therefore, research into effective ways to deal with coronavirus anxiety is needed.
To map or not to map the cN0 neck: impact of sentinel lymph node biopsy in canine head and neck tumours.

Tumor stage is a prognostic indicator for canine malignant head and neck tumors (MHNT). However, consensus is lacking on nodal staging in the absence of clinically apparent nodal disease (cN0 neck). This prospective observational study aims to determine the diagnostic accuracy of radiopharmaceutical and blue dye for sentinel lymph node biopsy (SLNB), to assess the correspondence between sentinel lymph node (SLN) and clinically expected regional lymph node (RLN) and the impact on staging of the procedure in dogs with MHNT and cN0 neck. Twenty-three dogs with MHNT and cN0 neck underwent tumour excision and SLNB guided by preoperative lymphoscintigraphy and intraoperative gamma-probe and blue dye. Diagnostic performances and detection rate were calculated. Correspondence between SLN and RLN, number of nodes excised, histopathological status of the SLN and complications related to the procedure were recorded. The mapping technique identified at least one SLN in 19/23 dogs, with a detection rate of 83%. The SLN did not correspond to the RLN in 52% of dogs. Multiple nodes were removed in 61% of dogs. At histopathology, 8 (42%) dogs had SLN+, of which 4 differed from the RLN. Only minor self-limiting complications occurred in 5 (22%) dogs. Radiopharmaceutical and blue dye guidance is accurate (sensitivity 88.9%; specificity 100%) for SLNB in dogs with MHNT and cN0 and allowed the extirpation of unpredictable and/or multiple SLN with minimal morbidity. Incorporation of SLNB in the management of MHNT is desirable to correctly stage the cN0 neck, owing the unpredictability of the lymphatic drainage.
A HORT1 Retrotransposon Insertion in the PeMYB11 Promoter Causes Harlequin/Black Flowers in Phalaenopsis Orchids1[OPEN]

Harlequin/black flowers of Phalaenopsis result from the insertion of a retrotransposon in the PeMYB11 promoter. The harlequin/black flowers in Phalaenopsis orchids contain dark purple spots and various pigmentation patterns, which appeared as a new color in 1996. We analyzed this phenotype by microscopy, HPLC, gene functional characterization, genome structure analysis, and transient overexpression system to obtain a better understanding of the black color formation in Phalaenopsis orchids. Most mesophyll cells of harlequin flowers showed extremely high accumulation of anthocyanins as well as a high expression of Phalaenopsis equestris MYB11 (PeMYB11) as the major regulatory R2R3-MYB transcription factor for regulating the production of the black color. In addition, we analyzed the expression of basic helix-loop-helix factors, WD40 repeat proteins, and MYB27- and MYBx-like repressors for their association with the spot pattern formation. To understand the high expression of PeMYB11 in harlequin flowers, we isolated the promoter sequences of PeMYB11 from red and harlequin flowers. A retrotransposon, named Harlequin Orchid RetroTransposon 1 (HORT1), was identified and inserted in the upstream regulatory region of PeMYB11. The insertion resulted in strong expression of PeMYB11 and thus extremely high accumulation of anthocyanins in the harlequin flowers of the Phalaenopsis Yushan Little Pearl variety. A dual luciferase assay showed that the insertion of HORT1 enhanced PeMYB11 expression by at least 2-fold compared with plants not carrying the insertion. Furthermore, the presence of HORT1 explains the high mutation rates resulting in many variations of pigmentation patterning in harlequin flowers of Phalaenopsis orchids.
Body image among adolescents and young adults diagnosed with cancer: A scoping review

Cancer and treatment can produce temporary or permanent body changes, which may affect the body image (BI) of adolescents and young adults diagnosed with cancer (AYAs). This evidence has not been comprehensively summarized. A scoping review was conducted to explore the available evidence on BI among AYAs and identify the definitions, theories, models, frameworks, measures, and methods used to assess BI.
Scale-up of Flame Aerosol Synthesis by Computational Fluid Dynamics

Synthesis of numerous high-performance materials for nano-enabled products and devices has been demonstrated with laboratory-scale flame aerosol reactors. Bringing such products to the market requires, however, cost-effective, large-scale production. For process scale-up, simulations reduce substantially the development time and costs compared to experimental trialand-error approach. Process models have to account for all important mechanisms influencing particle formation and growth to yield useful predictions and to elucidate the underlying phenomena that affects product characteristics. Appropriate simplifications, however, have to be made to limit the computational cost. As model results have to be compared to experiments, it is important that fluid, combustion and particle growth dynamics for exemplary benchmark processes are characterized unambiguously and with high accuracy. The validated model can then be used to determine optimal reactor geometry and process conditions for the desired application. Here, methods to measure and model flame aerosol synthesis at industrially relevant conditions are discussed.
Essential Oils in Experimental Models of Neuropsychiatric Disorders: A Systematic Review

Background Neural cells undergo functional or sensory loss due to neurological disorders. In addition to environmental or genetic factors, oxidative stress is a major contributor to neurodegeneration. In this context, there has been a growing interest in investigating the effects of EOs (EOs) in recent years, especially in the treatment of neuropathologies. The chemical and biological effects of EOs have led to important treatment tools for the management of various neurological disorders. Objective In the present study we performed a systematic review that sought to comprehend the neuroprotective effects of different EOs. Method This work is a systematic review where an electronic search was performed on PubMed, ScienceDirect, Cochrane Library and SciELO (Scientific Electronic Library Online) databases, covering the last 10 years, using “Essential oil” and “Neuroprotective effect” as reference terms. Results A total of 9 articles were identified, in which the efficacy of EOs was described in experimental models of anxiety, dementia, oxidative stress, cerebral ischemia, Alzheimer’s disease, and oxidative toxicity. Conclusion EOs from different species of medicinal plants have shown positive responses in neurological disorders such as anxiety, dementia, oxidative stress, cerebral ischemia, and oxidative toxicity. Thus, EOs emerges with the potential to be used as alternative agents in the treatment of neurological disorders.
Comparisons of 105Rh(III) Chloride Complexation with [14]aneNS3, [14]aneN2S2 and [14]aneN4 Macrocycles in Aqueous Solution

Rh(III) complexation was studied with two thiamacrocycle ligands (i.e., [14]aneNS3 and [14]aneN2S2). Rh(III) chloride forms a well-defined complex with the [14]aneNS3 macrocycle in high yields (93 ± 3% at pH 4) at low ligand concentrations (i.e., s 5 μπ ιο^) under relatively mild conditions. These results indicate the potential utility of using the [14]aneNS3 macrocycle frameworks as a basis to prepare high specific activity Rhbifunctional chelates. Under the same reaction conditions, Rh complexes with [14]aneN2S2 and [14]aneN4 ligands are formed, but in significantly lower yields (80 ± 3 and 54 ± 6% at pH 4, respectively). X-ray crystal structure studies demonstrate the presence of the cis Rh(III) dichloro core in both the {Rh(III)[14]aneN2S2Cl2} and {Rh(III)[14]aneNS3Cl2} complexes.
Assessment of the Effect of Different Preservatives on the Shell-Life of Soymilk Stored at Different Temperatures

This study examined theeffect ofdifferent preservatives on the shell-life ofsoymilkstored at different t emperatures.The utilization of soybeanfor the production of soy milkwas studied.Soy milk was extracted from whole and dehulled seed, pasteurized and fermented . All soy milk sampl es were analyzed for proximate composition (moisture%, ash %, total solids, fat) and the organoleptic tests (color, thickness, appearance, body, texture, taste, smell, flavor and overall acceptability) of the soy milk samples were evaluated to determine th e shelf-stability of the products during refrigeration and room temperature storage . The moisture, protein, fibre, fat, ash, carbohydrate and total solids of soy milk from whole and dehulled seed differed significantly (p<0.05). There was marked variationin the % fat content of the products. The results of the sensory evaluation revealed that flavor with respect to taste and smell had significant influence (p<0.05) on overall acceptability of soy milk product. The sensory properties of yam bean yoghurt sam ples were compared with soybean yoghurt. The sensory properties showed that sample stored at refrigeration temperature maintained good quality up to 16 days storage while samples stored at room temperature were of poor quality by the 4 th day. The implicat ion of these results is discussed. So, the soy milk manufacturers need to improve on the sensory properties in particular flavor and taste for better consumer acceptability. Also, they may improve on packaging by labeling to specifications that precisely r epresent the content and type. (Odu NN , Egbo NN and Okonko IO.Assessment of the Effect of Different Preservatives on the Shell-Life of Soymilk Stored at Different Temperatures . Researcher 2012;4(6):62-69). (ISSN: 1553-9865). http://www.sciencepub. net/researcher . 11
Low temperature helium pressure broadening of HCN

We measured the helium induced pressure broadening and shifting of the distinct hyperfine components of the j = 1 ← 0 and j = 2 ← 1 transitions of HCN at temperatures between 1.3 and 18 K. The HCN molecules were cooled to these temperatures using the collisional cooling technique. As a test that this cooling technique was generating the cold samples expected, we measured the Doppler contribution to the spectral lines, and these measurements confirmed that the molecules were at the same temperature as the walls of the spectroscopic cell. We observed that the hyperfine components of the 2← 1 transition have distinct broadening coefficients that differ from one another by as much as 5%. The measured differences are in reasonable agreement with theoretical predictions. We also performed molecular scattering calculations, using three proposed He-HCN potential energy surfaces, in order to compare our pressure broadening and shifting results with theoretical predictions. The predictions were within a few standard deviations near 18 K, but at the lowest experimental temperatures the predictions were many standard deviations larger than the measured values. This discrepancy is similar to a discrepancy found in two previous low temperature pressure broadening studies, and this dissertation discusses possible experimental and theoretical origins for this persistent discrepancy.