Miracle-dz/UniFAR-FTUs huggingface dataset:
ISOLATION, SCREENING AND CHARACTERIZATION OF L-ARGINASE PRODUCING SOIL BACTERIA

L-arginase (EC 3.5.3.1) is a therapeutic enzyme which catalyzes the hydrolysis of L-arginine to L-ornithine and urea. L-arginase is of keen interest due to its significance as an anti-cancer mediator against auxotrophic cancers. This enzyme is widely distributed and expressed in organisms like fungi, plants, non-chordates, and chordates, however very scarce report is available for L-arginase from bacteria. Therefore, the present study was aimed to isolate L-arginase producing bacteria from soil. Soil samples were collected from different locations in and around Karnatak University campus, Dharwad, Karnataka, India. L-arginase activity was confirmed by rapid plate assay screening using minimal arginine agar media and estimating the urea level colorimetrically. Among the sixty-eight isolates, twenty-nine of them were positive for the L-arginase production, but, the most potent isolate was selected, characterized, and identified in our study. The isolate was identified as Pseudomonas sp. strain PV1 (GenBank accession number MK459549.1). INTRODUCTION: L-arginase (EC 3.5.3.1) is a ureohydrolase enzyme that catalyzes L-arginine to ornithine and urea 1 . It is a crucial enzyme in the urea cycle, which facilitates the organism in detoxifying ammonia and excretion of excess nitrogen from the system 2 . It is widely distributed and expressed in different organisms 3 . L-arginase regulates the cellular levels of arginine through these metabolic pathways i. Regulates the level of L-arginine for the production of nitric acid, ii. Regulates the level of L-ornithine for the production of L-proline and polyamines to form collagen and facilitate cell growth and repair, respectively 4, 5 . L-arginase occurs in two forms in mammalian tissues, i.e., L-arginase I and II, which are coded by the genes located on chromosome 6 and 14 respectively 6 . QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.10(7). 3440-44 The article can be accessed online on www.ijpsr. com DOI link: http://dx.doi. org/10.13040/IJPSR.0975-8232.10(7). 3440-44 L-arginase has been reported to play a crucial role in the treatment of neurological disorders 7 , allergic asthma 8 , rheumatoid arthritis 9 , it is also found to have tumor inhibitory properties and has been accredited substantial consideration due to its extensive range of activity against cancer cells 10 . Certain cancers cannot biosynthesize arginine due to lack of expression of argininosuccinate synthetase-1 (ASS1). These cancers require arginine for proliferation and metastasis. L-arginine is catabolized into Lornithine and urea. Nutritional starvation due to lack of L-arginine leads to inhibition of DNA, RNA, and proteins biosynthesis, ultimately resulting in apoptosis of cancer cells. Normal cells can synthesize arginine due to which remain unaffected 11, 12 . This resulted in a persistent attempt to screen newer organisms to find strains capable of fabricating new L-arginase with great yield and fewer adverse properties. L-arginase has considerable anticancer potential; there are only a few investigations for sources of production of this enzyme.
Molar Fraction of Carbon, Nitrogen and Mineral Sources Regulate Antibiotic Biosynthesis Pattern in Pseudomonas Fluorescens CHA0

Fluorescent Pseudomonas species inhibit fungal phytopathogens under laboratory and greenhouse conditions, but in field trials the inconsistent performance is observed, which is thought to be due to variations in abiotic and biotic factors in/around the rhizosphere. Combination of nutrients and mineral components relevant to root zone and its effect on the antibiotic biosynthesis by fluorescent Pseudomonas species important for the understanding of biocontrol activity in the rhizosphere. Using Stat ease 8, and fractional factorial design sixteen combinations of four macronutrient (glucose, citrate, nitrate, phosphate) and three essential elements (Fe, Zn and Mo) obtained, and studied for the main effects and higher order interactions, on the culture growth, antifungal activity toward Rhizoctonia bataticola , and on the biosynthesis of pyrrolnitrin (PRN), 2, 4-diacetyphloroglucinol (DAPG) and pyoluteorin (PLT) in the model biocontrol strain Pseudomonas fluorescens CHA0 (Pf CHA0). growth, antifungal activity as well as antibiotic biosynthesis. Principal Component Analysis revealed that 2 day antifungal is positively correlation with PLT maximally followed by OD, PRN and DAPG. Fifth day antifungal activity showed positive correlation maximally with PRN followed by OD, and PLT. Bio-control supporting nutrient combinations showed effective protection of Vigna radiata from R. bataticola . Present study will be helpful for improving the reliability and application potential to the various soil types and different plant rhizosphere types. Present work establish the connection between multiple combinations of nutrients and edapic factors relevant to root zone and its effect on the growth, antifungal activity and biosynthetic of pyrrolnitrin, 2, 4-DAPG and pyoluteorin by Pf CHA0. Result showed that citrate, glucose, combination of citrate and glucose, ferrous iron and zinc have positive effect on growth, antifungal activity and antibiotic biosynthesis. Triple combination of citrate, glucose and nitrate inhibited the growth, antifungal activity and antibiotic biosynthesis. Antifungal activity is maximum contributed by pyoluteorin initially but by pyrrolnitrin later stage of growth. Bio-control supporting nutrient combinations showed effective protection of Vigna radiata from R.bataticola infection in plant inoculation assay.
The Effects of Coping Styles and Gender on Academic Procrastination among University Students

Background: Procrastination is one of troubling phenomena which affects millions of people all over the world. Academic procrastination is one of the most important reasons of failure or underachievement of university students. Some reasons can be put forward why university students rank highly among those mostly vulnerable to academic procrastination. Aim: The present study examined the relationships between coping style and gender on academic procrastination and their inter-relationship as common predicators of academic procrastination, regarding students' fields of study. Sample: 310 subjects were selected by multi-stage cluster sampling from among University of Mashhad students in Iran. Methodology: First of all, the Lay academic procrastination scale was translated from English into Persian. The results of the pilot study on 35 samples demonstrate an acceptable reliability of Lay academic procrastination for Iranian university students. The present study is a project of correlation method. Then, the Questionnaires of Lay academic procrastination and Andler-Parker coping style were administered to the subjects. Results: The data were analyzed using, mean, standard deviation, and one-way ANOVA, ttest and regression analyses. Analyses of the data using oneway ANOVA indicated significant differences among the three coping style groups. In general, the result of regression analysis showed that Problem-oriented coping style, inversely, is a significant predictor of academic procrastination. Furthermore, there was no significant difference among boys and girls, as far as the level of academic procrastination concerns. Conclusion: Findings suggest that coping strategies are affective factor to predictor of academic procrastination. Implications of the findings are discussed and some suggestions are made for the educator and counselors.
Risk, Trust, Shopping Enjoyment and Consumer Online Shopping Intention

Online consumer behaviour is an extensive and appealing area of study that can promote firms involved in marketing and selling the products online. Now days, consumers from non metro and tier II and tier III are also showing their interest in buying products. It is very essential to study that how consumers behave in online shopping which help out the retail organizations in targeting consumers and framing online marketing strategies. The purpose of this study is to examine consumers’ online shopping behaviour with a specific focus on the influence of shopping enjoyment, perceived risk and trust. Most of the past researches in this area have been conducted in the context of developed countries. However, examining consumers’ perceived Risk, shopping enjoyment and trust with online purchasing Indian context is very limited. The results of study of perceptions of 518 online consumers in Gujarat reveal that perceived risk online has an inverse relationship with consumers’ attitude and that attitude has a positive influence on intentions to continue purchasing online. On the other hand the shopping enjoyment and trust have a positive relationship with consumer’s attitude and that attitude has a positive influence on intentions to purchase online. In this study data is analyzed using AMOS 20 with the help of structural equation modeling. Keywords--Risk, Trust, Shopping Enjoyment, Online Shopping, Intention
SUBJECTIVE AND OBJECTIVE ORAL HEALTH INDICATORS AMONG INDIAN ADULT POPULATION

Background: technicians and labourers are under stress due to irregular work pattern and prolonged working hours, making this group vulnerable to change their regular dietary habits and life study was conducted to assess the impact of oral Central Sericulture, between clinical measure of oral Methods: was collected. A 14 used and clinical examination was done to record DMFT according to WHO criteria 1997. Results: 1.10±1.71, 0.49±1.06 and 3.57±3.19 respectively. The most prevalently affected OHIP physical pain (64.76%). Caries status was found to be significantly correlated with most of the domains of OHIP Conclusion: OHIP-
Socioeconomic Influence of Glaucoma on Patients

The aim of the study was to determine the socioeconomic influence of glaucoma on patients. In a descriptive cross sectional study, socio-economic data were collected from 150 glaucoma patients on treatment at Komfo Anokye Teaching Hospital (KATH) and St. Michael’s Catholic Hospital, in the Ashanti region, Ghana through interviews and questionnaire. All patients interviewed gave verbal consent to participate in this study. Out of the 150 participants 77 (51.33%) were females and 73 (48.67%) were males. Majority (55.33%) of the participants were not working (unemployed, aged or retired). Majority (70.67%) of the participants either earned a monthly income of less than GH¢ 400, did not a monthly income or could not account for their monthly income. One hundred and twelve (74.67%) of the participants reported the cost of management of their condition as a form of financial burden on them, 56.67% were afraid of getting blind, 51.33% were depressed, 46.00% had their lifestyle affected, 42.67% had their work affected and 40.00% couldn’t accomplish their daily task. The most significant socio-economic influence of glaucoma on the participants was the financial burden of the cost of management. The National Health Insurance Schemes (NHIS) should be restructured to cover more glaucoma medications and treatment to ease the financial burden on patients.
Design & Implementation of Fuzzy Inference System For Automatic Braking System

The significant application of fuzzy set theory and fuzzy logic are investigated. Attempt is made to simulate Fuzzy Logic Controller (FLC) for an Automatic Braking System. Keeping this idea in mind, an initiative is taken to design and simulate a Fuzzy Inference System (FIS) to perform Antiskid braking in a quasi - virtual environment. In this paper, the implementation of a Mamdani Fuzzy Inference System has been demonstrated with the application of an automatic braking system. The system consists of fuzzy logic controller that analyzes possible accident situation based on the inter-vehicle distance and their relative speed. The controller determines the sufficient brake pressure required to prevent collision while providing a smooth ride for the passengers in vehicle. This paper illustrates the simulation and design concepts of fuzzy logic controller for automatic braking system.
Sensor Based Automatic Irrigation Management System

In the field of agriculture, use of proper method of irrigation plays an important role for the economy and development of a country. In the conventional irrigation system, the farmer has to keep watch on irrigation timetable, which is different for different crops. This work makes the irrigation automated. With the use of low cost sensors and the simple circuitry this work aims low cost product, which can be bought even by a poor farmer. This work is best suit for places where water is scarce and has to be used in limited quantity. Also, the third world countries can afford this simple and low cost solution for irrigation and obtain good yield on crops.PIC controller that is be used in this work. A 16×4 LCD is connected to the microcontroller, which displays the soil moistures level and switches are provided to set the limits of humidity for switching the individual solenoid valves controlling the water flow to the field. The humidity and temperature levels are transmitted at regular time interval to the LCD through a serial port for data display and analysis. The humidity sensors are constructed using aluminum sheets and housed in easily available materials. The aim is to use the readily available material to construct low cost sensors. Relays are controlled by the microcontroller through the high current driver IC and provided for controlling solenoid valves, which controls the flow of water to different parts of the field. Other relay is used to shut-off the main motor which is used to pump the water to the field. Performance of sensors in terms of energy consumption has also been analyzed.
RENOPROTECTIVE EFFECT OF OCIMUM SANCTUM IN COMPARISON WITH OLMESARTAN MEDOXOMIL AND PITAVASTATIN IN METFORMIN TREATED DIABETIC RATS

Nephropathy is an important comorbidity associated with diabetes leading to chronic kidney disease. We studied the effect of Ocimum sanctum in comparison to Olmesartanmedoxomil, Pitavastatin and their combination in prevention of this comorbidity. Male Wistar rats were given streptozotocin to induce diabetes and randomly divided into groups. Group 1 (Control group) received Metformin (94.5mg/kg/day)+Distilled Water, Group 2 received Metformin (94.5mg/kg/day) +Ocimum sanctum (250 mg/kg/day), Group 3 received Metformin (94.5mg/kg/day) + Olmesartanmedoxomil(1.80 mg/kg/day), Group 4 received Metformin (94.5mg/kg/day) + Pitavastatin (0.18 mg/kg/day) and Group 5Metformin (94.5mg/kg/day) + Olmesartanmedoxomil (1.80 mg/kg/day) + Pitavastatin (0.18 mg/kg/day). The effects of Metformin alone and with Ocimum sanctum, Olmesartanmedoxomil, Pitavastatin and their combination on blood glucose and renal function test parameters were assessed. All groups showed decrease in fasting plasma glucose and glycosylated hemoglobin. Serum urea and creatinine were increased in the Control group. However, significant improvement in all the parameters studied were seen when Ocimum sanctum, Olmesartan or Pitavastatin were added. Histopathological examination of kidney showed mesangial proliferation, tubular swelling, thickened glomerular basement membrane, hypertrophy in glomerular tufts andhydropic degeneration in control group and improvement in all treatment groups. The best results were seen in Ocimum sanctum and Olmesartan+Pitavastatin groups. Metformin alone does not prevent derangement of renal function and histological changes in the kidney in diabetes mellitus. These changes can be prevented by addition of Olmesartan and Pitavastatin or Ocimum sanctum. INTRODUCTION: Diabetes mellitus (DM) is a global health crisis, which has been persistently affecting humanity, irrespective of the socioeconomic profile and geographic location of the population. As per the American Diabetes Association, diabetes mellitus is a group of metabolic disorder characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. It is a complex group of disorders that disturbs the metabolism of carbohydrates, fat and protein and is characterized by increased fasting and postprandial blood sugar levels 1 .
A Network Attack Classification for Multi Agent System Using Flow Based Intusion Detection System

— Intrusion detection system is precious for protecting the entire network from a large range of threats, modern intrusion detection techniques must cope with not only increase the detection rate but also increase the speed of the network line, signature based ids forced to sample meagerly, increasing the probability of harmful traffic flowing towards the network without inspection subsequently, flow based id is attaining awareness as an effective complement. Basically ids are classified in to network based or host based The independent multi agent design idea is a scalable, striking option for its potential to influence the strengths of both architectures: the broad perspective and visibility into distributed harmful activity provided by network-based ID, and the complete view of the local node provided by host-based ID. This paper develops an architecture for an new multi agent, flow based intrusion detection system. The architecture is designed in two iterations of increasing complexity. These innovative designs are used to find nodes by “reputation “concept that are most effective for classifying harmful network activity. Every system has to design which includes the growth of an innovative classifier that uses multi point evolutionary algorithms to assist in the search for efficient functioning parameter values. Extensive agent simulation framework which highlights the condition under the Reputation System provides a Classification.
Rainfall Trend Detection in Northern Nigeria over the Period of 1970-2012

This study examined the trends in variability and spatial distribution of annual rainfall over northern Nigeria during the period 1970-2012 with a view to understand the pattern of rainfall trend (significance and magnitude), by applying various statistical tools on the data obtained from 11 weather stations. The non-parametric Mann–Kendall test was used to determine the statistical signi? cance of trends while the magnitude of trends was derived from the Sen slope estimator of the linear trends using Kendall robust line ? tting. Map of rainfall trends was generated by applying a geo-statistical interpolation technique to visualize the detected tendencies. The findings revealed that a significant positive increase of 2.16mm in rainfall was recorded in the entire northern Nigeria within the period of 1970 to 2012. It further indicated that majority of the stations revealed an upward trend, with Bauchi, Borno, Kebbi and Sokoto stations showing significant positive trends of 8.13mm, 4.30mm, 4.76mm and 4.42mm respectively. It is concluded that there is high variability in rainfall in the northern Nigeria which signifies a clear evidence of climate change in the region. Keywords: Rainfall trend, Man-Kendall test, Northern Nigeria, Climate change
STROKE AND ITS RISK PROFILE IN WESTERN NEPAL

Stroke is a common cause of morbidity and mortality. In Nepal, there is paucity of data on stroke prevalence and its associated risk factors. This study was carried out to obtain the clinical profile of patients suffering from various stroke subtypes in western Nepal. This is a hospital-based cross-sectional descriptive study which aims to assess the clinical profile of stroke patients admitted at Lumbini Medical College and Teaching Hospital which caters services to rural area of western Nepal. A total number of 224 cases of stroke were included in this study during the period from January 2014 to June 2015 who was admitted in internal medicine ward. Mean age of patients was 62.92 (range from 34 to 84 years) with male and female ratio of 1.64. Amongst strokes, hemorrhagic stroke was more common than ischemic stroke (48.21% vs 42.85%). Subarachnoid hemorrhage was observed in 8.92% of stroke patients. The common manifestations were motor disturbances, altered sensorium, headache and speech abnormality. The common predisposing factors were smoking, hypertension and alcohol consumption. The most common site of both hemorrhagic and ischemic stroke was basal ganglia. Among hemorrhagic strokes, 33.33% of patients had ventricular extension of bleeding. Most commonly involved territory was middle cerebral artery. The burden of stroke and its associated risk factors is high in western part of Nepal. Nationwide campaign for early detection and management of hypertension, diabetes mellitus and for smoking cessation and alcohol abstinence can help reduce the prevalence of stroke.
Effects of peer education on AIDS knowledge and sexual behavior among youths on national service and secondary school students in Nigeria

Young persons are disproportionately affected by the impact of HIV in Nigeria. This paper presents findings from an evaluation of a national youth HIV prevention program designed to determine the effects of HIV prevention intervention (HPI) on Youths on Compulsory National Service (YoCNS) and Adolescents in Secondary Schools (AiSS). Data were collected from 229 YoCNS who received training on HIV prevention and 231 of their counterparts who did not. Among AiSS, data were collected from 909 respondents who were trained as peer educators and among 1005 students who were not. Data were collected in six states namely Akwa-Ibom, Enugu, Gombe, Kaduna, Plateau and Osun using a questionnaire that explored knowledge on HIV and sexual behavior and supplemented by focus group discussions and in-depth interviews. Results show that both youths and students who received HPI reported superior knowledge on HIV/AIDS than their counterparts who did not. Students in the intervention sites also reported fewer numbers of multiple sexual partners (9.7%) compared to those in the comparison sites (15.4%). The qualitative data shows that the project had positive effects on both youths and students who received the intervention. Given these positive outcomes, it is recommended that the project should not only be sustained but expanded to reach adolescents who are out of school.
Can We Improve the Birth Weight Prediction Using the SymphysisFundal-HeightMesaurement? An Exploratory Prospective Cohort Study inSpain

Objective: The construction of a predictive model that improves the estimation of the fetal weight (EFW). Study Design: a comparative, descriptive study. One hundred forty pregnant women were recruited at two-stage sample in health department in Spain. They were classified in four groups depending on the pre-gestational BMI. Fetal weight was estimated by ultrasound at 35-40 weeks (EFW40w) by one gynecologist. A regression model was created with the variables that reacted to the newborn´s weight, symphysis-fundal height (SFH), EFW40w, gestational age (GA), ferritin level and cigarettes smoked. Results: A multivariatel model was created for the NW group to estimate the fetal weight (EFWme), resulting in R2=0.727 (p<0.001). The differences of the averages obtained between EFW40w and EFWme, with the new born´s weight were significant (p<0.001). EFWme underestimates birth weight by 0.07 g (mean error 0.53%), and EFW40w overestimates it by 300.89 g (mean error 10.12%). In order to evaluate the predictive model and verify the predictions we used the Bland-Altman analysis. The average error in estimating the birth weight with EFWme was 1.94% underestimating the result, whereas the ultrasound error overestimated the result 10.93%. Conclusion: The multivariate model created for the NW group improves the accuracy of the ultrasound.