Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical Investigations of CAR-T Cell Therapy for Solid Tumors

Cell therapy is a distinguished targeted immunotherapy with great potential to treat solid tumors in the new era of cancer treatment. Cell therapy products include genetically engineered cell products and non-genetically engineered cell products. Several recent cell therapies, especially chimeric antigen receptor (CAR)-T cell therapies, have been approved as novel treatment strategies for cancer. Many clinical trials on cell therapies, in the form of cell therapy alone or in combination with other treatments, in solid tumors, have been conducted or ongoing. However, there are still challenges since adverse events and the limited efficacy of cell therapies have also been observed. Here, we concisely summarize the clinical milestones of the conducted and ongoing clinical trials of cell therapy, introduce the evolution of CARs, discuss the challenges and limitations of these therapeutic modalities taking CAR-T as the main focus, and analyze the disparities in the regulatory policies in different countries.
An Investigation for Skin Tissue Regeneration Enhancement/Augmentation by Curcumin-Loaded Self-Emulsifying Drug Delivery System (SEDDS)

Diabetes, one of the global metabolic disorders, is often associated with delayed wound healing due to the elevated level of free radicals at the wound site, which hampers skin regeneration. This study aimed at developing a curcumin-loaded self-emulsifying drug delivery system (SEDDS) for diabetic wound healing and skin tissue regeneration. For this purpose, various curcumin-loaded SEDDS formulations were prepared and optimized. Then, the SEDDS formulations were characterized by the emulsion droplet size, surface charge, drug content/entrapment efficiency, drug release, and stability. In vitro, the formulations were assessed for the cellular uptake, cytotoxicity, cell migration, and inhibition of the intracellular ROS production in the NIH3T3 fibroblasts. In vivo, the formulations’ wound healing and skin regeneration potential were evaluated on the induced diabetic rats. The results indicated that, after being dispersed in the aqueous medium, the optimized SEDDS formulation was readily emulsified and formed a homogenous dispersion with a droplet size of 37.29 ± 3.47 nm, surface charge of −20.75 ± 0.07 mV, and PDI value of less than 0.3. The drug content in the optimized formulation was found to be 70.51% ± 2.31%, with an encapsulation efficiency of 87.36% ± 0.61%. The SEDDS showed a delayed drug release pattern compared to the pure drug solution, and the drug release rate followed the Fickian diffusion kinetically. In the cell culture, the formulations showed lower cytotoxicity, higher cellular uptake, and increased ROS production inhibition, and promoted the cell migration in the scratch assay compared to the pure drug. The in vivo data indicated that the curcumin-loaded SEDDS-treated diabetic rats had significantly faster-wound healing and re-epithelialization compared with the untreated and pure drug-treated groups. Our findings in this work suggest that the curcumin-loaded SEDDS might have great potential in facilitating diabetic wound healing and skin tissue regeneration.
Sleep and behavioral problems in preschool-age children with Down syndrome

Sleep is a major concern, especially in people with Down Syndrome (DS). Beyond Obstructive Sleep Apnea, a number of other sleep difficulties have been reported in children with DS, such as delayed sleep onset, night-time awakenings, and early morning awakenings. The detrimental effect of sleep difficulties seems to contribute to and exacerbate the cognitive and behavioral outcomes of DS. Although the screening for sleep disorders is recommended early in age in DS, only a few studies have evaluated the sleep profile in preschool-age children with DS. The aim of the current study was to assess the association between sleep disturbances and behavioral problems in a group of preschool-age children with DS, by means of a feasible and easy-to-administer parent-report questionnaires. Seventy-one preschool-age children with DS, ranging in age from 3 to 5.11 years, were included in this retrospective study. Sleep disturbances were evaluated by means of the Sleep Disturbance Scale for Children, while emotional and behavioral problems by means of the Child Behavior Checklist. Sleep breathing disorders were the most frequent sleep difficulties reported by parents. Moreover, children with clinical scores in total sleep problems exhibited elevation of psychopathological symptoms, namely Total problems, Affective problems, Anxiety problems, Pervasive Developmental Problems, and Attention Deficit/Hyperactivity Problems. The identification of the broader connection between sleep difficulties and emotional and behavioral problems in preschool-age children with DS leads to important considerations for intervention.
Electroencephalogram-Based Motor Imagery Signals Classification Using a Multi-Branch Convolutional Neural Network Model with Attention Blocks

Brain signals can be captured via electroencephalogram (EEG) and be used in various brain–computer interface (BCI) applications. Classifying motor imagery (MI) using EEG signals is one of the important applications that can help a stroke patient to rehabilitate or perform certain tasks. Dealing with EEG-MI signals is challenging because the signals are weak, may contain artefacts, are dependent on the patient’s mood and posture, and have low signal-to-noise ratio. This paper proposes a multi-branch convolutional neural network model called the Multi-Branch EEGNet with Convolutional Block Attention Module (MBEEGCBAM) using attention mechanism and fusion techniques to classify EEG-MI signals. The attention mechanism is applied both channel-wise and spatial-wise. The proposed model is a lightweight model that has fewer parameters and higher accuracy compared to other state-of-the-art models. The accuracy of the proposed model is 82.85% and 95.45% using the BCI-IV2a motor imagery dataset and the high gamma dataset, respectively. Additionally, when using the fusion approach (FMBEEGCBAM), it achieves 83.68% and 95.74% accuracy, respectively.
Resilient Mechanical Metamaterial Based on Cellulose Nanopaper with Kirigami Structure

Nanopapers fabricated from cellulose nanofibers (CNFs) are flexible for bending while they are rather stiff against stretching, which is a common feature shared by conventional paper-based materials in contrast with typical elastomers. Cellulose nanopapers have therefore been expected to be adopted in flexible device applications, but their lack of stretching flexibility can be a bottleneck for specific situations. The high stretching flexibility of nanopapers can effectively be realized by the implementation of Kirigami structures, but there has never been discussion on the mechanical resilience where stretching is not a single event. In this study, we experimentally revealed the mechanical resilience of nanopapers implemented with Kirigami structures for stretching flexibility by iterative tensile tests with large strains. Although the residual strains are found to increase with larger maximum strains and a larger number of stretching cycles, the high mechanical resilience was also confirmed, as expected for moderate maximum strains. Furthermore, we also showed that the round edges of cut patterns instead of bare sharp ones significantly improve the mechanical resilience for harsh stretching conditions. Thus, the design principle of relaxing the stress focusing is not only important in circumventing fractures but also in realizing mechanical resilience.
Extracellular Vesicles Inhibit the Response of Pancreatic Ductal Adenocarcinoma Cells to Gemcitabine and TRAIL Treatment

Pancreatic ductal adenocarcinoma remains an aggressive cancer with a low 5-year survival rate. Although gemcitabine has been a standard treatment for advanced pancreatic cancer, patients often develop resistance to this therapeutic. We have previously shown that treating pancreatic cancer cells in vitro with a combination of gemcitabine and the cytokine TRAIL significantly reduced both cell viability and survival. The data presented here demonstrate that this response to treatment is inhibited when cells are incubated with a conditioned medium derived from untreated cells. We show that this inhibition is specifically mediated by extracellular vesicles present in the conditioned medium, as seen by a significant decrease in apoptosis. Additionally, we further demonstrate that this effect can be reversed in the presence of GW4869, an inhibitor of exosome biogenesis and release. These results show that pancreatic cancer cell-derived extracellular vesicles can confer resistance to treatment with gemcitabine and TRAIL. The implications of these findings suggest that removal of EVs during treatment can improve the response of cells to gemcitabine and TRAIL treatment in vitro.
Attachment Style and Its Relationships with Early Memories of Separation Anxiety and Adult Separation Anxiety Symptoms among Emerging Adults

Emerging adulthood concerns the transition from adolescence to adulthood. It foresees the separation from the family and the creation of new significant relationships, whereby specific attachment styles might be triggered when facing these challenges. The present study investigates the influence of retrospective memories associated with childhood separation anxiety symptoms upon emerging adults’ romantic avoidant vs. anxious attachment styles including adult separation anxiety symptoms as mediators. Age and gender were included as covariates. A community sample of N = 394 Italian emerging adults (Mage = 23.64, SD = 4.00, 70% females) completed self-report measures. The results showed that the participants presented a greater anxious attachment rather than an avoidant attachment style. Moreover, both adult separation anxiety and the memories of early separation anxiety were positively and significantly associated with anxious attachment and not with avoidant attachment. A mediation model conducted and focused on anxious attachment showed that, although not directly associated, child separation anxiety did show a significant positive indirect effect on anxious attachment as mediated by adult separation anxiety symptoms. Thus, the findings highlighted the influence of adult separation anxiety symptoms and retrospective childhood memories of separation anxiety upon anxious adult romantic attachment, yet not on avoidant attachment style. Clinical implications are discussed and suggestions for future research are provided.
Validation of a New Telenursing Questionnaire: Testing the Test

Background: Existing surveys on telenursing refer to specific areas of nursing after the implementation of a programme, but telenursing in general has not been fully evaluated from a prospective approach. Aim: Design and statistical validation of a telenursing questionnaire. Methods: A new questionnaire was designed with 18 paired (to avoid leading) questions (Likert-5) plus three dichotomous questions (randomly ordered, inspired by existing validated tests) to analyse the dimensions of: acceptance, usefulness and appropriateness of telenursing from the nursing point of view (7 min test). The questionnaire was validated by classical tests and item response tests (Rasch) using six computer-generated databases with different response profiles (tendency to be positioned against, neutral and positioned in favour) with two degrees of agreement between each pair of responses for each option. Results: Classical testing: Cronbach’s alphas (from 0.8 to 0.95), Kaiser–Meyer–Olkin (KMO) (0.93 to 0.95) and a significant p < 0.0001 for Bartlett’s test of sphericity were obtained. Rasch analysis: Reliability coefficients (0.94). Warm’s mean weighted likelihood estimates (0.94). Extreme infit-t and outfit-t values (+1.61 to −1.98). Conclusions: Both the classical test and the Rasch approaches confirm the usefulness of the new test for assessing nurses’ positioning in relation to telenursing.
Comparisons of Hematological and Biochemical Profiles in Brahman and Yunling Cattle

Simple Summary In order to evaluate the health condition and differences between Brahman cattle and Yunling cattle, 28 adult Brahman and 65 adult Yunling cattle were analyzed from the same farm with standard grazing management. We detected 55 hematological and biochemical parameters using an automatic biochemical analyzer. The results showed that 27 hematological and biochemical parameters of Brahman cattle were lower than those of Yunling cattle, whereas the other indices detected were higher. Based on the hematological and biochemical parameters investigated, the Yunling cattle may have a better physical condition than Brahman cattle, such as having a greater metabolism and better liver function. Thus, these data suggest that, as a hybrid breed that is half Brahman, Yunling cattle are superior in their abilities of adaptability, stress resistance and tolerance to crude feed. Therefore, our study reveals the underlying molecular basis of Yunling cattle’s better performance at adapting to local environments, and maintaining beef production and reproduction traits. Abstract Brahman cattle are tolerant to parasite challenges and heat stress. Yunling cattle are three-way hybrids that are half Brahman cattle, a fourth Murray Grey cattle and a fourth Yunnan Yellow cattle, with good beef performance. The hematological and biochemical parameters can reflect the physiology and metabolic conditions of cattle, and there are valuable indicators of production performance and adaptability that can be found by studying the cattle. To assess the health status and differences, we compared 55 hematological and biochemical parameters of 28 Brahman cattle and 65 Yunling cattle using an automatic biochemical analyzer. Our results showed that 27 hematological and biochemical indices of Brahman cattle were lower than those of Yunling cattle, whereas the other parameters were higher. There are 20 indices with significant differences that were detected between Brahman and Yunling cattle (with p ≤ 0.01 or 0.01 ≤ p ≤ 0.05, respectively), and no significant differences were found for other indices (p > 0.05). Based on these results, Yunling cattle may have a better physical condition than Brahman cattle, may be better at adapting to local environments, and can maintain a good production and reproduction performance. As a new breed that is half Brahman, the abilities of Yunling cattle, including adaptability, stress resistance and tolerance to crude feed, were better than Brahman cattle under the same management conditions.
Erosion Map Reliability Using a Geographic Information System (GIS) and Erosion Potential Method (EPM): A Comparison of Mapping Methods, BELGRADE Peri-Urban Area, Serbia

Soil erosion is a product of natural and anthropogenic factors and, at the same time, an economic and environmental concern. One of the methods applied to calculate the intensity of erosion is the erosion potential method (EPM), with two possible procedures for determining the average erosion coefficient of an area: analytical and graphical. Using GIS and EPM methods, without field observations of erosion, based on cartographic materials and satellite images, erosion maps were created for 1970 and 2018, for part of the peri-urban area of Belgrade. Based on the created erosion maps, the values of the mean coefficients of erosion, as well as the arithmetic means for the study area, were determined for the settlements. The aim of the study is to assess the reliability of the mean coefficient of soil erosion, obtained from the erosion map created from the cartographic materials and satellite images, without field observations of erosion. Thus, the obtained values of the mean erosion coefficient were compared with the values obtained from the erosion map with field observation and the values obtained by the analytical procedure. Statistical analysis (F test) for 1970 and for 2018 determined a high degree of reliability (p < 0.05) of the mean erosion coefficients of the area obtained from erosion maps that were created from cartographic materials and satellite images without field observation. Regardless of the procedure for determining the mean erosion coefficient, a significant decrease in soil losses was observed, from 10.64 to 5.97 t ha−1year−1 (average annual specific production of sediments, year 1970 and 2018, respectively).
Energy Efficiency of the Baltic Sea Countries: An Application of Stochastic Frontier Analysis

Using the stochastic frontier analysis (SFA) model, this research measures total-factor energy efficiency (TFEE) and disaggregate input efficiency for 10 countries across the Baltic Sea from 2004 to 2014. Real capital, labor, energy use, and carbon dioxide (CO2) are input variables, real gross domestic product (GDP) is the output variable, and renewable energy consumption and urban population are the environmental variables. The results provide not only the TFEE scores, in which statistical noise is considered, but also the determinants of inefficiency, which show the following. (i) Norway, Sweden, Finland, and Latvia perform better with respect to energy efficiency than other countries in the Baltic Sea Region. (ii) Interestingly, the average energy use efficiency scores from 2004 to 2014 in the 10 Baltic countries exhibit a gradual upward trend except for 2009. (iii) For the inefficiency estimates, higher renewable energy consumption and urban population correspond to higher TFEE scores.
Ultra-Precision Cutting and Characterization of Reflective Convex Spherical Blazed Grating Elements

In this work, based on the diffraction principle of reflective blazed grating, the structure size of the convex spherical blazed grating unit is determined, the machining accuracy of the convex spherical blazed grating is formulated, the effects of tool nose radius and Poisson burr on the diffraction efficiency of the convex spherical blazed grating are analyzed, and the performances of cutting convex gratings with microcrystalline aluminum RSA6061 and RSA6061+ chemically plated NiP for two workpiece materials are compared. A convex spherical blazed grating with a radius of curvature R = 41.104 mm, substrate diameter 14 mm, grating density 53.97 line/mm, and blaze angle of roughly 3.8° is turned by a four-axis ultra-precision machining system by adjustment of the cutting tool, workpiece material, and cutting parameters, as well as modification of the layouts of the blazed grating on the convex sphere. The results of the testing of convex spherical blazed grating elements in both layouts show that the size error of the grating period is close for both layouts, the size error of grating height is smaller in the equal-along-arc layout, the blaze angle error in the equal-along-projection layout is only 0.74%, and the average roughness of the blazed surface is less than 5 nm to meet the processing quality requirements of the reflective convex spherical blazed grating. The greater the blaze angle accuracy of the blazed grating, the higher its diffraction efficiency, so the grating element with an equal-along-projection layout has a higher diffraction efficiency than the grating element with an equal-along-arc layout. RSA6061+ chemically plated NiP material is superior to RSA6061 material in Poisson burr height and blazed surface roughness, which is more suitable for Offner-type imaging spectrometers in the spectral range 0.95–2.5 μm (SWIR).
Role of Dual Inoculation of Rhizobium and Arbuscular Mycorrhizal (AM) Fungi on Pulse Crops Production

Legume crops are useful as human and animal feed, wood energy, and as soil-improving components of agricultural and agro forestry systems through its association with bio-fertilizers. The later have a potential environment friendly inputs that are supplemented for proper plant growth. Bio-fertilizers are preparations containing living cells of microorganisms that help crop plants in the uptake of nutrients by their interactions in the rhizosphere. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts for plant growth. They are associated with higher plants by a symbiotic association and benefit plants in the uptake of phosphorus nutrients, production of growth hormones, increase of proteins, lipids and sugars levels, helps in heavy metal binding, salinity tolerance and disease resistance. In nature symbiotic association of Rhizobium and leguminous plants fixes atmospheric nitrogen. Indeed, research has proved that the association of mycorrhizae fungi and Rhizobium , with pulse crops, increased the beneficial aspects comparatively more than their single associations with the host plants. This review focuses on the role of dual inoculation of AM fungi and Rhizobium on different pulse crops.
IT reliability and the results of accredited laboratories functioning in Poland

The article indicates the need to apply IT solutions in research and reference laboratories operating in Poland in the context of measurable benefits. The results of empirical studies carried out in 84 laboratories are presented. Most of the surveyed entities declare the use of standard (typical) information systems, and almost three-fifths use specialized software dedicated to the laboratory's field of activity. Oddly enough, entities declaring the implementation of typical IT solutions have lower performance parameters than those that do not. The analysis confirms that the use of (reliable) IT in research and reference laboratories allows to achieve measurable benefits in these entities in all areas of their functioning. Reliable IT supports, among others, processes in the laboratory (including identification and elimination of possible disturbances), has a positive impact on the quality of services, management efficiency and increases the level of competitiveness. Streszczenie. W artykule wskazano na konieczność stosowania rozwiązań IT w laboratoriach badawczych i wzorcujących funkcjonujących w Polsce w kontekście możliwych do uzyskania, wymiernych korzyści. Przedstawiono wyniki badań empirycznych przeprowadzonych w 84 laboratoriach. Większość badanych podmiotów (trzy czwarte) deklaruje stosowanie standardowych (typowych) systemów informacyjnych, a prawie trzy piąte posługuje się specjalistycznym oprogramowaniem dedykowanym dla obszaru działalności laboratorium. Co dość dziwne, organizacje deklarujące wdrożenie typowych rozwiązań IT odnotowują niższe parametry wynikowe niż te, które ich nie stosują. Analiza potwierdza, że stosowanie niezawodnego IT w laboratoriach badawczych i wzorcujących pozwala na osiągniecie w tych podmiotach wymiernych korzyści we wszystkich obszarach ich funkcjonowania. Niezawodne IT sprzyja m.in. prawidłowym przebiegom procesów w laboratorium (w tym identyfikacji i eliminacji ewentualnych zakłóceń) wpływa pozytywnie na jakość usług, sprawność zarządzania i podniesienie poziomu konkurencyjności laboratoriów. Wzrostowi niezawodności IT towarzyszy wzrost parametrów wynikowych funkcjonowania badanych laboratoriów (Niezawodność IT a rezultaty akredytowanych laboratoriów badawczych i wzorcujących funkcjonujących w Polsce)