Miracle-dz/UniFAR-FTUs huggingface dataset:
Mesenchymal Stem Cell Secretome Enhancement by Nicotinamide and Vasoactive Intestinal Peptide: A New Therapeutic Approach for Retinal Degenerative Diseases

Mesenchymal stem cells (MSC) secrete neuroprotective molecules that may be useful as an alternative to cell transplantation itself. Our purpose was to develop different pharmaceutical compositions based on conditioned medium (CM) of adipose MSC (aMSC) stimulated by and/or combined with nicotinamide (NIC), vasoactive intestinal peptide (VIP), or both factors; and to evaluate in vitro their proliferative and neuroprotective potential. Nine pharmaceutical compositions were developed from 3 experimental approaches: (1) unstimulated aMSC-CM collected and combined with NIC, VIP, or both factors (NIC+VIP), referred to as the aMSC-CM combined composition; (2) aMSC-CM collected just after stimulation with the mentioned factors and containing them, referred to as the aMSC-CM stimulated-combined composition; and (3) aMSC-CM previously stimulated with the factors, referred to as the aMSC stimulated composition. The potential of the pharmaceutical compositions to increase cell proliferation under oxidative stress and neuroprotection were evaluated in vitro by using a subacute oxidative stress model of retinal pigment epithelium cells (line ARPE-19) and spontaneous degenerative neuroretina model. Results showed that oxidatively stressed ARPE-19 cells exposed to aMSC-CM stimulated and stimulated-combined with NIC or NIC+VIP tended to have better recovery from the oxidative stress status. Neuroretinal explants cultured with aMSC-CM stimulated-combined with NIC+VIP had better preservation of the neuroretinal morphology, mainly photoreceptors, and a lower degree of glial cell activation. In conclusion, aMSC-CM stimulated-combined with NIC+VIP contributed to improving the proliferative and neuroprotective properties of the aMSC secretome. Further studies are necessary to evaluate higher concentrations of the drugs and to characterize specifically the aMSC-secreted factors related to neuroprotection. However, this study supports the possibility of improving the potential of new effective pharmaceutical compositions based on the secretome of MSC plus exogenous factors or drugs without the need to inject cells into the eye, which can be very useful in retinal pathologies.
The Origin of a Distributed Stellar Population in the Star-forming Region W4

Stellar kinematics provides the key to understanding the formation process and dynamical evolution of stellar systems. Here, we present a kinematic study of the massive star-forming region (SFR) W4 in the Cassiopeia OB6 association using the Gaia Data Release 2 and high-resolution optical spectra. This SFR is composed of a core cluster (IC 1805) and a stellar population distributed over 20 pc, which is a typical structural feature found in many OB associations. According to a classical model, this structural feature can be understood in the context of the dynamical evolution of a star cluster. The core-extended structure exhibits internally different kinematic properties. Stars in the core have an almost isotropic motion, and they appear to reach virial equilibrium given their velocity dispersion (0.9 ± 0.3 km s−1) comparable to that in a virial state (∼0.8 km s−1). On the other hand, the distributed population shows a clear pattern of radial expansion. From the N-body simulation for the dynamical evolution of a model cluster in subvirial state, we reproduce the observed structure and kinematics of stars. This model cluster experiences collapse for the first 2 Myr. Some members begin to radially escape from the cluster after the initial collapse, eventually forming a distributed population. The internal structure and kinematics of the model cluster appear similar to those of W4. Our results support the idea that the stellar population distributed over 20 pc in W4 originate from the dynamical evolution of IC 1805.
Systematic review of combined functional near-infrared spectroscopy and transcranial direct-current stimulation studies

Abstract. Significance: Combining transcranial direct-current stimulation (tDCS) with functional near-infrared spectroscopy (fNIRS) is a recent approach to exploring brain activation evoked by neurostimulation. Aim: To critically evaluate studies combining tDCS and fNIRS and provide a consolidated overview of cortical hemodynamic responses to neurostimulation. Approach: Key terms were searched in three databases (MEDLINE, EMBASE, and PsycINFO) with cross-referencing and works from Google Scholar also evaluated. All studies reporting on fNIRS-derived hemoglobin changes evoked by tDCS were included. Results: Literature searches revealed 474 articles, of which 28 were included for final review (22 in healthy individuals: 9 involving rest and 13 with tasks; 6 in the clinical setting). At rest, an overall increase in cortical activation was observed in fNIRS responses at the site of stimulation, with evidence suggesting nonstimulated brain regions are also similarly affected. Conversely, during tasks, reduced cortical activation was observed during online stimulation. Offline and poststimulation effects were less consistent, as is the impact on clinical populations and their symptom correlation. Conclusion: This review explores the methodological frameworks for fNIRS-tDCS evaluations and summarizes hemodynamic responses associated with tDCS in all populations. Our findings provide further evidence of the impact of tDCS on neuronal activation within functionally connected networks.
Quantification of Macular Microvascular Changes in Retinitis Pigmentosa Using Optical Coherence Tomography Angiography

Purpose To investigate macular structural and microvascular changes in retinitis pigmentosa (RP) eyes compared to age-matched controls using spectral domain optical coherence tomography (SD-OCT) and OCT angiography (OCTA). Methods This was a cross-sectional study. The foveal and parafoveal thickness and extent of ellipsoid zone (EZ) disruption were measured on OCT. The foveal avascular zone (FAZ) area and retinal vessel density (VD) were automatically calculated for superficial (SCP) and deep capillary plexuses (DCP) and choriocapillaris using OCTA-integrated software. VD was assessed for the whole 6 × 6 mm image, foveal and parafoveal regions. Quantitative measures were compared between healthy and diseased eyes. Results The study included 30 eyes of 30 RP patients and 24 eyes of 24 controls. Mean age was 26.9±6.4 years for RP patients and 27.4±4.9 years for controls (p=0.76). Mean LogMAR visual acuity (VA) was 0.9±0.5 for RP patients and 0.05±0.05 for controls (p <0.001). Foveal and parafoveal thicknesses were significantly lower in RP cases as compared to the control group. EZ disruption was observed in RP cases only (869±211 µm). Mean FAZ area was significantly larger in RP eyes in both SCP and DCP. VD was significantly reduced in RP eyes at the level of SCP, DCP and choriocapillaris. VA, deep parafoveal VD, foveal, parafoveal and whole image choriocapillaris VD were negatively correlated with the extent of EZ disruption. Conclusion We report OCTA findings in a relatively young cohort of RP patients. We demonstrated a reduction of retinal microvascular density in all studied layers on OCTA. We believe studying retinal vasculature in these patients is important, as a healthy blood supply is a prerequisite for the success of new cell-based therapies under trial for RP.
Effect of weather parameters on population dynamics of green leaf hopper and white backed plant hopper in paddy grown in middle Gujarat region

Nephotettix virescens Distant and Sogatella furcifera (Horv) commonly known as Green leaf hopper (GLH) and White backed plant hopper (WBPH) respectively. These are the serious pests of kharif paddy all over the world causing extensive losses. The population dynamics of Green leaf hopper and white backed plant hopper for ten consecutive years (1994 to 2004) except for 1997 were correlated with the weather parameters like maximum and minimum temperature, rainfall, relative humidity and bright sunshine hours. The results revealed that the bright sunshine hours had a positive significant correlation (r=0.166) with the population dynamics of GLH. The correlation between WBPH peak population and bright sunshine hours also showed positive significantcorrelation (r=0.269), while maximum temperature, minimum temperature, rainfall and relative humidity showed non-significant effect on population build up of both GLH and WBPH. Green leaf hopper attained peak population during 43rdstandard meteorological week; whereas white backed plant hopper reached peak population during 39th standard meteorological week and decreased considerably thereafter.
Pathological Role of Angiotensin II in Severe COVID-19

Abstract The activated renin–angiotensin system induces a prothrombotic state resulting from the imbalance between coagulation and fibrinolysis. Angiotensin II is the central effector molecule of the activated renin–angiotensin system and is degraded by the angiotensin-converting enzyme 2 to angiotensin (1–7). The novel coronavirus infection (classified as COVID-19) is caused by the new coronavirus SARS-CoV-2 and is characterized by an exaggerated inflammatory response that can lead to severe manifestations such as acute respiratory distress syndrome, sepsis, and death in a proportion of patients, mostly elderly patients with preexisting comorbidities. SARS-CoV-2 uses the angiotensin-converting enzyme 2 receptor to enter the target cells, resulting in activation of the renin–angiotensin system. After downregulating the angiotensin-converting enzyme 2, the vasoconstrictor angiotensin II is increasingly produced and its counterregulating molecules angiotensin (1–7) reduced. Angiotensin II increases thrombin formation and impairs fibrinolysis. Elevated levels were strongly associated with viral load and lung injury in patients with severe COVID-19. Therefore, the complex clinical picture of patients with severe complications of COVID-19 is triggered by the various effects of highly expressed angiotensin II on vasculopathy, coagulopathy, and inflammation. Future treatment options should focus on blocking the thrombogenic and inflammatory properties of angiotensin II in COVID-19 patients.
Evaluation of Disease Resistance of the Fish Lake–DeSmet, Wounded Man, and Harrison Lake Strains of Rainbow Trout Exposed to Myxobolus cerebralis

Abstract Several tests evaluated the whirling disease resistance of three stocks of rainbow trout Oncorhynchus mykiss: the Fish Lake–DeSmet (FD), Wounded Man (WM), and Harrison Lake (HL) strains. In one series, FD fish were exposed to Myxobolus cerebralis triactinomyxons (TAMs; one exposure of 1,000 TAMs/fish). Histological scores ranged from 4.28 to 4.95 (MacConnnell−Baldwin scale) among FD fish from three different hatcheries. In addition, FD fish were exposed either acutely (1,000 TAMs/fish in one dose) or chronically (100 or 200 TAMs/fish on each of 10 d). There was no significant difference in mean myxospore counts among the three different exposure treatments. Histological scores ranged from 3.76 to 3.86 among the exposure groups and did not differ among exposure treatments. The correlation between histological scores and myxospore counts was significant (r = 0.57). Three different ages of WM rainbow trout were challenged with 1,000 TAMs/fish; histological scores for 62-d-old fish were significantly.
Social Phobia and Its Impact on Quality of Life Among Regular Undergraduate Students of Mettu University, Mettu, Ethiopia

Background Social anxiety disorder is a serious and disabling mental health problem that begins before or during adolescence, with the potential to significantly interfere with an individual’s daily functioning and overall quality of life. Objective The aims of this study were to assess the prevalence, severity, and quality of life towards social anxiety disorder among students of Mettu University, Ethiopia. Subjects and Methods A cross-sectional study was conducted among a stratified sample of 523 undergraduate students to identify the prevalence, correlates of social anxiety disorder, and impacts on quality life. All participants completed the Social Phobia Inventory, Liebowitz Social Anxiety Scale, and World Health Organization Quality of Life-Brief Form, Turkish Version (WHOQOL-BREF-TR). Of 523 students, 26% were screened positive for social anxiety disorder. About 69.4% and 17.4% of the students had mild and moderate symptoms of social anxiety disorder, respectively. WHOQOL BREF-TR scores showed that students with social phobia had significantly lower quality of life quality than those without social phobia. Being criticized by others or fear of parties was the most commonly feared situations. Talking to strangers was the most commonly avoided situations. Being females, current tobacco use, and family history of psychiatric illness were factors significantly associated with social phobia symptoms using logistic regression analysis. Conclusion The current study shows high prevalence of social phobia among the university students and its significant negative effects on quality of life which require prompt identification and treatment.
Serum Hepatitis B Virus RNA Levels Predict HBeAg Seroconversion and Virological Response in Chronic Hepatitis B Patients with High Viral Load Treated with Nucleos(t)ide Analog

Background and Aim Hepatitis B virus (HBV) RNA has attracted increasing attention as a novel serum marker for intrahepatic HBV replication. However, the predictive value of the serum level of HBV RNA for hepatitis B e-antigen (HBeAg) seroconversion and viral response among patients with a high viral load (HVL) is unclear. We evaluated the role of the serum level of HBV RNA as a predictor of treatment response in chronic HBV (CHB) patients with an HVL. Patients and Methods The study cohort was 66 HBeAg-positive CHB patients with an HVL (serum HBV DNA >1.9×106 IU/mL) at baseline from our previous prospective cohort study treated with lamivudine (LAM) and adefovir dipivoxil(ADV) (N=31) or entecavir alone (N=35) for ≤96 weeks. The serum HBV RNA level was quantified by TaqMan® probe-based reverse transcription real-time quantitative polymerase chain reaction at four time points. Results The baseline serum HBV RNA level (in log10 copies/mL) in patients treated with LAM+ADV and ETV monotherapy was 8.97±1.22 and 9.15±0.92, respectively. After nucleos(t)ide analog (NA) therapy, the serum HBV RNA level decreased steadily in all patients (week 0 vs week 12, p<0.001; week 12 vs week 24, p=0.010; week 24 vs week 48, p<0.001). Fifty-three (80.3%) patients achieved a virologic response (VR), and 12 (18.2%) achieved HBeAg seroconversion after 96 weeks. Multivariate analyses revealed that the serum HBV RNA level at week 12 could predict HBeAg seroconversion (OR 3.560, 95% CI: 1.39–9.110, p=0.008) and VR (1.908, 1.115–3.265, 0.018) at 96 weeks. Analyses of receiver operating characteristic curves indicated that the serum HBV RNA level 12 weeks after NA treatment had predictive value for HBeAg seroconversion (AUC=0.847, p<0.001) and VR (AUC=0.736, p=0.011). Conclusion The serum level of HBV RNA at 12 weeks could predict HBeAg seroconversion and a VR during NA treatment in CHB patients with an HVL.
EDITOR'S CHOICE: Evaluating marine spatial closures with conflicting fisheries and conservation objectives

Summary Spatial management is used extensively in natural resource management to address sustainability and biodiversity issues, for example through declaration of terrestrial National Parks and marine protected areas (MPAs). Spatial management is used also to optimize yields or protect key parts of the life cycle of species that are utilized (hunted, farmed or fished), for example through rotational harvesting. To evaluate the effectiveness of marine spatial closures with conflicting fisheries and conservation objectives, a series of marine fisheries closures are here analysed using an integrative modelling tool known as management strategy evaluation (MSE). This modelling framework combines a food web model of a tropical ecosystem fished by a prawn (shrimp) fishery that emulates the resource being managed, together with the present management system and risk-based tools of fishing the prawn species at maximum economic yield. A series of spatial closures are designed and tested with the aim of investigating trade-offs among biodiversity (MPA), benthic impacts, ecosystem function, key species at risk to fishing, economic and sustainability objectives. Synthesis and applications. This paper illustrates that existing tools often available in actively managed fisheries can be linked together into an effective management strategy evaluation framework. Spatial closures tended to succeed with respect to their specific design objective, but this benefit did not necessarily flow to other broad-scale objectives. This demonstrates that there is no single management tool which satisfies all objectives, and that a suite of management tools is needed.
Quantification of Synergistic Effects of Ceragenin CSA-131 Combined with Iron Oxide Magnetic Nanoparticles Against Cancer Cells

Background Therapeutic efficiency of ceragenins against cancers may be limited by lack of their hemocompatibility when high concentrations of molecules are required to reach a desired result. Synergistic effects observed upon administration of anticancer agents and metal nanoparticles may provide an opportunity to limit toxicity of immobilized ceragenins on the surface of metal nanoparticles and to improve their therapeutic efficiency at the same time. The aim of present work is to investigate the anticancer activities and hemocompatibility of nanoformulations consisting of ceragenin CSA-131 united with aminosilane-modified iron oxide-based magnetic nanoparticles (MNP) and prepared by 1) covalent bonding (MNP@CSA-131) or 2) by combining CSA-131 with MNP in 1:1 ratio (CSA-131 + MNP). Possible synergistic interactions between CSA-131 and magnetic nanoparticles were also quantified. Methods MNP@CSA-131 and CSA-131+MNP were tested in vitro against selected lung and colon cancer cells using colorimetric, fluorimetric and flow cytometry methods. Results Performed analysis demonstrates that MNP-based nanosystems significantly improve the killing efficiency of tested ceragenin, decreasing the viability of extra 1.37±4.72% to 76.07±15.30% cancer cells when compared to free CSA-131. Quantification of synergistic effects indicates the favorable interactions between CSA-131 and magnetic nanoparticles (CI < 1 for all tested doses), revealing at the same time a reduction in effective doses of ceragenin from 1.17 ± 0.61 to 34.57 ± 12.78 times when combined with MNP. We demonstrate that both MNP@CSA-131 and CSA-131+MNP induce significantly apoptosis of cancer cells and prevent the division of colon cancer cells even at relatively low doses of the active compound (10 µg/mL). Importantly, combining CSA-131 with MNP decreases the hemolytic activity of free ceragenin 4.72 to 7.88 times, which indicates a considerable improvement of hemotoxicity profile. Conclusion Comparative analyses have revealed that both developed CSA-containing nanoformulations due to the utility of synergistic interactions between MNP and CSA-131, which are effective against lung and colon cancer cells. This indicates the new directions in preparation of MNP-based therapeutics, which are relatively easy to synthetize, cost-effective and safe when intravenously administrated.
Correlation Decay and Partition Function Zeros: Algorithms and Phase Transitions

We explore connections between the phenomenon of correlation decay and the location of Lee-Yang and Fisher zeros for various spin systems. In particular we show that, in many instances, proofs showing that weak spatial mixing on the Bethe lattice (infinite $\Delta$-regular tree) implies strong spatial mixing on all graphs of maximum degree $\Delta$ can be lifted to the complex plane, establishing the absence of zeros of the associated partition function in a complex neighborhood of the region in parameter space corresponding to strong spatial mixing. This allows us to give unified proofs of several recent results of this kind, including the resolution by Peters and Regts of the Sokal conjecture for the partition function of the hard core lattice gas. It also allows us to prove new results on the location of Lee-Yang zeros of the anti-ferromagnetic Ising model. We show further that our methods extend to the case when weak spatial mixing on the Bethe lattice is not known to be equivalent to strong spatial mixing on all graphs. In particular, we show that results on strong spatial mixing in the anti-ferromagnetic Potts model can be lifted to the complex plane to give new zero-freeness results for the associated partition function. This extension allows us to give the first deterministic FPTAS for counting the number of $q$-colorings of a graph of maximum degree $\Delta$ provided only that $q\ge 2\Delta$. This matches the natural bound for randomized algorithms obtained by a straightforward application of Markov chain Monte Carlo. We also give an improved version of this result for triangle-free graphs. Finally, on trees of maximum degree $\Delta$, we show zero-freeness of the anti-ferromagnetic Potts model partition function under the condition $q \geq \Delta + 1$, which matches the threshold for weak spatial mixing.
Five Serum Trace Elements Associated with Risk of Cardia and Noncardia Gastric Cancer in a Matched Case–Control Study

Background Serum trace elements have for some time been suggested to influence the development of gastric cancer, but evidence is still lacking. Methods All newly diagnosed patients with gastric cancer were compared with healthy controls 1:1 matched by sex, age (±3 years), and place of residence during 2013–2015. The serum concentration of all trace elements was analyzed using inductively coupled plasma mass spectrometry. Heliobacter pylori infections in cases were diagnosed using the rapid urease test, while in controls were detected using the colloidal gold method. Results A total of 122 cases of gastric cardia cancer (GCC) and 177 gastric noncardia cancer (NCGC), and 299 matched healthy controls were included. Positive associations were found between serum copper and copper/zinc ratio and risk of overall gastric cancer (OR4th vs 1st quartile: 2.42, 95% CI: 1.10–5.32 for copper; OR4th vs 1st quartile: 11.7, 95% CI: 3.83–35.6 for copper/zinc ratio), and for both GCC and NCGC subtypes. Serum selenium was inversely associated with the risk of NCGC (OR4th vs 1st quartile: 0.18, 95% CI: 0.07–0.51), while molybdenum seems to reduce the risk of GCC (OR4th vs 1st quartile: 0.12, 95% CI: 0.03–0.47). Strong inverse associations were also detected between serum calcium and risk of all groups of gastric cancer (all p for trend<0.05). Conclusion The current study indicated statistically significant positive associations between serum copper, copper/zinc ratio, and gastric cancer, as well as inverse associations among selenium, molybdenum, and calcium. The results shall be carefully interpreted before further in vivo animal studies show definitive evidence.