Miracle-dz/UniFAR-FTUs huggingface dataset:
Chromosome instability inCandidaalbicans

Candida albicans maintains genetic diversity by random chromosome alterations, and this diversity allows utilization of various nutrients. Although the alterations seem to occur spontaneously, their frequencies clearly depend on environmental factors. In addition, this microorganism survives in adverse environments, which cause lethality or inhibit growth, by altering specific chromosomes. A reversible loss or gain of one homolog of a specific chromosome in this diploid organism was found to be a prevalent means of adaptation. We found that loss of an entire chromosome is required because it carries multiple functionally redundant negative regulatory genes. The unusual mode of gene regulation in Candida albicans implies that genes in this organism are distributed nonrandomly over chromosomes.
Three-dimensional induced polarization data inversion for complex resistivity

The conductive and capacitive material properties of the subsurface can be quantified through the frequency-dependent complex resistivity. However, the routine three-dimensional interpretation of voluminous induced polarization (IP) data sets still poses a challenge due to large computational demands and solution non-uniqueness. In this work, we propose a flexible methodology for three-dimensional (spectral) IP data inversion. Our inversion algorithm is adapted from a frequency-domain electromagnetic (EM) inversion method primarily developed for large-scale hydrocarbon and geothermal energy exploration purposes. The method has proven to be efficient by implementing the non-linear conjugate gradient method with hierarchical parallelism and by using an optimal finite-difference forward modeling mesh design scheme. The method allows for a large range of survey scales, providing a tool for both exploration and environmental applications. We experiment with an image focusing technique in order to improve the poor depth resolution of surface data sets with small survey spreads. The algorithm’s underlying forward modeling operator properly accounts for EM coupling effects, thus traditionally used EM coupling correction procedures are not needed. The methodology is applied to both synthetic and field data. The benefit of directly inverting EM coupling contaminated data is demonstrated on a synthetic large-scale exploration data set. Afterwards, we demonstrate the monitoring capability of our method by inverting time-lapse data from an environmental remediation experiment near Rifle, Colorado. Similar trends observed in both our solution and another two-dimensional inversion are in accordance with previous findings about the IP effects due to subsurface microbial activity.
NDT_A_238985 1139..1144

Department of Child Healthcare, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, People’s Republic of China Purpose: Although emerging evidence has suggested that apolipoprotein C-III (APOC-III) is involved in the pathogenesis of Alzheimer’s disease (AD), the association of APOC-III with longitudinal changes in cerebrospinal fluid (CSF) AD pathologies (β-amyloid (Aβ42) and tau proteins) is not clear. In the present study, we aimed to examine whether plasma APOC-III levels are associated with longitudinal changes in CSF Aβ42, total-tau (t-tau), and phosphorylated-tau (p-tau) levels among older individuals without dementia. Patients and Methods: Linear mixed models were fitted with plasma APOC-III used as a predictor for longitudinal changes in CSF AD biomarkers over a 7-year period. Data were obtained from the Alzheimer’s Disease Neuroimaging Initiative database, and 195 older individuals without dementia (47 subjects with normal cognition (NC) and 148 subjects with mild cognitive impairment (MCI)) with baseline plasma APOC-III measurements were included. Results: Among older individuals without dementia, we found that the tertiles of plasma APOC-III were associated with changes in CSF Aβ42, but not t-tau or p-tau. Specifically, the CSF Aβ42 reduction for individuals in the highest plasma APOC-III tertile was significantly slower compared with those in the middle tertile, whereas no other pairwise difference was found to be statistically significant. Conclusion: Among older individuals without dementia, higher plasma APOC-III levels were associated with slower declines in CSF Aβ42.
Richardson, Sol and Langley, Tessa and Szatkowski, Lisa and Sims, Michelle and Gilmore, Anna and McNeill, Ann and Lewis, Sarah (2014) How does the emotive content of televised anti-smoking mass media campaigns influence monthly calls to the NHS Stop

Objective: To investigate the effects of different types of televised mass media campaign content on calls to the English NHS Stop Smoking helpline. Method: We used UK government-funded televised tobacco control campaigns from April 2005 to April 2010, categorised as either “positive” (eliciting happiness, satisfaction or hope) or “negative” (eliciting fear, guilt or disgust). We built negative binomial generalised additive models (GAM) with linear and smooth terms for monthly per capita exposure to each campaign type (expressed as Gross Ratings Points, or GRPs) to determine their effect on calls in the same month. We adjusted for seasonal trends, inflation-adjusted weighted average cigarette prices and other tobacco control policies. Results: We found non-linear associations between exposure to positive and negative emotive campaigns and quitline calls. The rate of calls increased more than 50% as exposure to positive campaigns increased from 0 to 400 GRPs (rate ratio: 1.58, 95% CI: 1.25–2.01). An increase in calls in response to negative emotive campaigns was only apparent after monthly exposure exceeded 400 GRPs. Conclusion: While positive campaigns were most effective at increasing quitline calls, those with negative emotive content were also found to impact on call rates but only at higher levels of exposure.
Effect of poly(ethylene glycol) on the properties of mixed matrix membranes for improved filtration of highly concentrated oily solution

Mixed matrix membrane (MMM) composed of organic and inorganic materials has been widely studied for its potential use in water and wastewater treatment, owing to its improved properties compared to the pristine membrane. In this work, the filtration performance of MMM composed of polyethersulfone (PES) and hydrous manganese oxide (HMO) nanoparticles was further improved by adding pore forming agent poly(ethylene glycol) (PEG) into dope solution to improve membrane hydrophilicity and structural morphology so as the developed membranes are suitable for handling highly concentrated oily solution (30,000 ppm oil concentration). Compared to the structure of control PES membrane that was composed of irregular microvoids, the presence of PEG and hydrophilic nanomaterial in the MMM was able to form extended finger-like structure from top to the bottom section of membrane and enhance its surface hydrophilicity, significantly improving water permeability. The improved water flux of MMM did not compromise the rejection rates of oil and chemical oxygen demand (COD) as the MMMs achieved comparable separation efficiency like the control membrane. The findings of this work revealed the potential use of MMM for the treatment of highly concentrated oily wastewater.
Evaluating the Viability of Merger Arbitrage in Nordic Equities

This thesis aims to examine whether a merger arbitrage strategy is able to generate market neutral alpha in the Nordic region. Similar studies of merger arbitrage strategies in both the US and Australian market find market neutral alpha. To investigate the viability of such a strategy, we developed a “Merger arbitrage portfolio” which invests in 55 deals during 2003-2017 in the Nordic equity capital market. Our findings provide strong support that a merger arbitrage strategy is market neutral, even in times of financial turmoil. An excess return is recorded, however, when estimating the portfolio with the Market Model we find no statistically significant alpha. The results are affected by large outliers. We conclude that our version of the merger arbitrage strategy is not an optimal investment in terms of its Sharpe Ratio, compared to an index using a similar strategy and the stock market.
Co-stimulatory blockade of CD154:CD40 in combination with T-cell lymphodepletion results in prevention of allogeneic sensitization Running Title: CD154 blockade prevents allogeneic sensitization

Sensitization is a critical unresolved challenge in transplantation. We show for the first time that blockade of CD154 alone or combined with T-cell depletion prevents sensitization. Allogeneic skin grafts were rejected by recipients treated with anti-αβ-TCR, anti-CD154, antiOX40L, or anti-ICOS mAb alone with a kinetic similar to untreated recipients. However, the production of anti-donor-MHC antibody was prevented in mice treated with anti-CD154 mAb only, suggesting a specific role for the CD154:CD40 pathway in B-cell activation. The impairment of T-cell-dependent B-cell responses by blocking CD154 occurs through inhibiting activation of T and B-cells and secretion of IFN-γ and IL-10. Combined treatment with both antiCD154 and anti-αβ-TCR abrogated anti-donor antibody production and resulted in prolonged skin graft survival, suggesting the induction of both Tand B-cell tolerance with prevention of allogeneic sensitization. In addition, we show that the tolerance induced by combined treatment was nondeletional. Moreover, these sensitization-preventive strategies promote bone marrow engraftment in recipients previously exposed to donor alloantigen. These findings may be clinically relevant to prevent allosensitization with minimal toxicity and point to humoral immunity as playing a dominant role in alloreactivity in sensitized recipients. 2 For personal use only. on August 18, 2017. by guest www.bloodjournal. org From
Factors Associated with Delayed Hepatitis B Viral Suppression on Tenofovir Among HBV-HIV Coinfected Patients in the CNICS Cohort

Background—Despite widespread use in HIV and hepatitis B virus (HBV) infection, the effectiveness of tenofovir (TDF) has not been studied extensively outside of small HBV-HIV coinfected cohorts. We examined the effect of prior lamivudine treatment (3TC) and other factors on HBV DNA suppression with TDF in a multi-site clinical cohort of coinfected patients. Methods—We studied all patients enrolled in the Centers for AIDS Research Network of Integrated Clinical Systems cohort from 1996-2011 who had chronic HBV and HIV infection, initiated a TDF-based regimen continued for ≥3 months and had on-treatment HBV measurements. We used Kaplan-Meier curves and Cox-Proportional hazards to estimate time to suppression (HBV DNA level <200 IU/ml or <1000 copies/ml) by selected covariates. Results—Among 397 coinfected patients on TDF, 91% were also on emtricitabine or 3TC concurrently, 92% of those tested were HBeAg-positive, 196 (49%) had prior 3TC exposure; 192 (48%) achieved HBV DNA suppression over a median of 28 months (IQR 13-71). Median time to HBV DNA suppression was 17 months for those who were 3TC-naïve and 50 months for those who were 3TC-exposed. After controlling for other factors, prior 3TC exposure, baseline HBV Corresponding author: H. Nina Kim, MD MSc University of Washington, 325 Ninth Avenue Box 359930, Seattle, WA 98104 (hyangkim@uw.edu). Alternate corresponding author: Mari M. Kitahata, MD MPH University of Washington, 325 Ninth Avenue Box 359931, Seattle, WA 98104 (kitahata@uw.edu). Potential conflicts of interest. JJE is a consultant to Gilead and Glaxo-Smith Klein. ETO is a consultant for Gilead and receives research funding from Gilead. MM has been a consultant for Gilead and Bristol-Myers Squibb (BMS) and has received research funding from BMS. Remaining authors have no potential conflicts of interest. This work was presented in part at the 20th Conference on Retroviruses and Opportunistic Infections 2013 in Atlanta, GA, 4 March 2013 (abstract 667). NIH Public Access Author Manuscript J Acquir Immune Defic Syndr. Author manuscript; available in PMC 2015 May 01. Published in final edited form as: J Acquir Immune Defic Syndr. 2014 May 1; 66(1): 96–101. doi:10.1097/QAI.0000000000000126. N IH PA Athor M anscript N IH PA Athor M anscript N IH PA Athor M anscript DNA level >10,000 IU/ml, and lower nadir CD4 count were independently associated with decreased likelihood of HBV DNA suppression on TDF. Conclusion—These results emphasize the role of prior 3TC exposure and immune response on delayed HBV suppression on TDF.
WOX4 promotes procambial development

Plant shoot organs arise from initial cells that are recruited from meristematic tissues. Previous studies have shown that members of the WUSCHEL -related homeob OX ( WOX ) gene family function to organize various initial cell populations during plant development. The function of the WOX4 gene is previously undescribed in any plant species. Comparative analyses of WOX4 transcription and function are presented in Arabidopsis thaliana , a simple-leafed plant with collateral vasculature, and in tomato ( Solanum lycopersicum ), a dissected-leafed species with bicollateral venation. WOX4 is transcribed in the developing vascular bundles of root and shoot lateral organs in both Arabidopsis and tomato. RNAi-induced down-regulation of WOX4 in Arabidopsis generated small plants whose vascular bundles accumulated undifferentiated ground tissue and exhibited severe reductions in differentiated xylem and phloem. In situ hybridization analyses of At wox4 - RNAi plants revealed delayed and reduced expression of both the phloem developmental marker ALTERED PHLOEM1 ( APL1 ) and of HOMEOBOX GENE 8 ( AtHB8 ) , a marker of the vascular procambium. Over-expression of SlWOX4 correlated with over-proliferation of xylem and phloem in transgenic tomato seedlings. The cumulative data suggest that the conserved WOX4 function is to promote differentiation and/or maintenance of the vascular procambium, the initial cells of the developing vasculature.
Where are protons and deuterons in KH p D 1 − p CO 3 ? A neutron diffraction study

The crystals of potassium hydrogen carbonate (KHCO3) and the KDCO3 analogue are isomorphous. They are composed of hydrogen or deuterium bonded centrosymmetric dimers (HCO−3 )2 or (DCO − 3 )2. The space group symmetry of KHpD1−pCO3 (p ≈ 0.75) determined with neutron diffraction is identical to those of KHCO3 and KDCO3. This is at variance with a random distribution of H and D nuclei. These crystals are macroscopic quantum systems in which protons or/and deuterons merge into macroscopic states. Widmung It is a great pleasure to dedicate this paper to Professor Hans-Heinrich Limbach on the occasion of his sixty fifth birthday, and to wish him many more happy and productive years of activity in chemistry. 2 ha l-0 03 69 69 7, v er si on 1 23 M ar 2 00 9
Physiological Effects of Green-Colored Food-Derived Bioactive Compounds on Cancer

Green-colored foods, such as broccoli, sprouts, soybean, and green leafy vegetables are considered one of the representative healthy foods for containing various functional ingredients that can combat chronic diseases, including diabetes, obesity, and cancer. Herein, we reviewed the anti-cancer activities and the underlying mechanisms of some important bioactive compounds, such as sulforaphane, catechins, chlorophyll, isoflavone, indole dervatives, and lutein, present in green-colored foods. In vivo and clinical studies suggest that sulforaphane, a sulfur-containing compound found in cruciferous vegetables, can ameliorate prostate and breast cancer symptoms by arresting cell-cycle progression and modulating Ki67 and HDAC expression. A green tea compound, known as epigallocatechin-3-gallate (EGCG), has shown remarkable anti-cancer effects against prostate cancer and lung adenocarcinoma in human trials through its antioxidative defense and immunomodulatory functions. Chlorophyll, a natural pigment found in all green plants, can regulate multiple cancer-related genes, including cyclin D1, CYP1A, CYP1B1, and p53. Epidemiological studies indicate that chlorophyll can substantially reduce aflatoxin level and can mitigate colon cancer in human subjects. Remarkably, the consumption of soy isoflavone has been found to be associated with the lower incidence and mortality of breast and prostate cancers in East Asia and in Canada. In vivo and in vitro data point out that isoflavone has modulatory effects on estrogen and androgen signaling pathways and the expression of MAPK, NfκB, Bcl-2, and PI3K/AKT in different cancer models. Other green food bioactive compounds, such as indole derivatives and lutein, also exhibited suppressing effects in rodent models of lung, liver, stomach, cervical, and prostate cancers. In addition, some micronutrients, such as folate, riboflavin, retinoic acid, and vitamin D3 present in green foods, also showed potential cancer suppressing effects. Taken together, these data suggest potential chemopreventive functions of the bioactive compounds from green-colored foods. This paper could be beneficial for further research on the anti-carcinogenic effects of green-colored food-derived compounds, in order to develop green chemotherapeutics for cancers.
Uptake and Metabolism of " C-Labeled Oleic Acid by Atherosclerotic Lesions in Rabbit Aorta

The uptake of C-labeled oleic acid and its incorporation into combined lipids by aortic intimas from normal and cholesterol-fed rabbits has been investigated in vitro. More than five times as much oleic acid was taken up by the atherosclerotic intima as by the normal intima. About twice as much oleic acid was incorporated into phospholipid, and twenty times as much into cholesterol ester by the atherosclerotic intima as by the normal. Lecithin was the major phospholipid synthesized from oleic acid in both normal and atherosclerotic intimas. Radioautographs of the atherosclerotic vessels show that the C-labeled oleic acid and its metabolic derivatives, principally phospholipid and cholesterol ester, were localized in sudanophilic cells in the intima in both early and advanced lesions. It is concluded that intimal foam cells are primarily responsible for the lipid synthesis that occurs in the atherosclerotic lesion. ADDITIONAL
Manuscript title: Bilingualism effects on the cognitive flexibility of autistic children: Evidence from verbal dual-task paradigms Abbreviated title: Cognitive flexibility in bilingual autistic children

The deficit in cognitive flexibility, i.e. the ability to adapt cognitive behavior to changing contexts, is one of the most prominent characteristics of autistic individuals. Inflexibility may manifest in restricted interests and increased susceptibility to the effects of misinformation either through inefficient inhibition of non-target information or deficient recall of correct information. Bilingualism has been shown to enhance executive functions in both typically-developing children and autistic children, yet, the effect of bilingualism on cognitive flexibility in autism remains underexplored. In this study, we used verbal dual-tasks to compare cognitive flexibility across 50 monolingual autistic and 50 bilingual autistic children, and 50 monolingual and 50 bilingual typically-developing children. The children were also administered language ability tests and a nonverbal global-local cognitive flexibility task, in order to investigate whether performance in the dual-tasks would be modulated by the children’s language and executive function skills. The bilingual autistic children outperformed their monolingual autistic peers in the dual tasks. The strength of the bilingualism effect, however, was modulated by the type of language processing that interfered with the target information in each dual-task, which suggests that the bilingual autistic children calibrated their processing resources and efficiently adapted them to the changing demands of the dual-task only to the extent that the task did not exceed their language abilities. Bilingual autistic children relied on their executive functions rather than on their language abilities while performing in the dual-tasks. The overall results show that bilingualism compensates for the reduced cognitive flexibility in autism. Manuscript Click here to access/download;Manuscript;manuscript.docx D ow naded rom httpdirect.m it.edu/nol/article-pdf/doil_a_00055/1962704/nol_a_00055. pdf by gest on 28 Sptem er 2021
DDDT_A_269236 4845..4854

Department of Reproduction and Genetics, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi 046000, People’s Republic of China Background: MicroRNAs play important roles in testicular development and spermatogenesis. Previous research has indicated that the level of miR-200a was significantly upregulated in patients with different spermatogenic impairments. However, the mechanism by which miR-200a regulated spermatogenic impairments remains unclear. Methods: Leydig cells were treated with triptolide (TP) to mimic spermatogenic impairments. CCK-8 and flow cytometry were used to detect the proliferation and apoptosis in Leydig cells, respectively. In addition, Western blot assay was used to examine ATG7, ATG5, p62 protein levels in MLTC-1 cells. Results: TP dose-dependently upregulated the expression of miR-200a in MLTC-1 cells. In addition, TP inhibited the proliferation of MLTC-1 cells via inducing apoptosis and oxidative stress; however, these phenomena were notably reversed by miR-200a antagomir. Furthermore, luciferase reporter assay identified that ATG7 was the direct binding target of miR-200a. TP treatment markedly inhibited the activation of autophagy in MLTC-1 cells via inhibition of ATG7. Conversely, downregulation of miR-200a significantly induced autophagy in TP-treated MLTC-1 cells by activation of ATG7. Meanwhile, the cell protective effects of miR-200a against TP were reversed by autophagy inhibitor 3MA, indicating that autophagy plays an important role. Conclusion: These results indicated that downregulation of miR-200a could protect MLTC1 cells against TP by inducing autophagy. Therefore, miR-200a might serve as a new therapeutic target for the treatment of male hypogonadism.
Upregulation of long noncoding RNA LINC00152 promotes proliferation and metastasis of esophageal squamous cell carcinoma

Purpose: Esophageal cancer is a major cause of cancer-related mortality worldwide. The long noncoding RNA LINC00152 has been con ﬁ rmed to play an oncogenic role in many cancers. However, the expression pattern and function of LINC00152 in human esophageal squamous cell carcinoma (ESCC) remain unclear. Materials and methods: We evaluated LINC00152 expression in ESCC by qPCR and in situ hybridization. Proliferation, apoptosis, cell cycle, migration and invasion were examined in ESCC cells knocked down for LINC00152 knockdown by siRNA. Furthermore, an mRNA microarray was performed in ESCC cells with LINC00152 knockdown. Results: LINC00152 was signi ﬁ cantly upregulated in human ESCC clinical samples ( P <0.001) and cell lines ( P =0.008), and LINC00152 overexpression was related to lymphatic metastasis ( P =0.03) and advanced pTNM classi ﬁ cation ( P =0.005). Furthermore, ESCC patients with LINC00152 overexpression had signi ﬁ cantly shorter overall survival ( P =0.007), and LINC00152 overexpression was an independent risk factor for overall survival of ESCC patients. LINC00152 knockdown inhibited the proliferation, migration and invasion of ESCC cells in vitro. In addition, mechanistic investigations through mRNA array and immunoblot analyses demonstrated that LINC00152 regulated the expression of several cell cycle-related proteins and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) interactions in vesicular transport pathway proteins. Conclusion: Our research indicated that LINC00152 exhibits oncogenic functions in ESCC and may represent a potential new target for ESCC therapy.