Miracle-dz/UniFAR-FTUs huggingface dataset:
Oncometabolite L‐2‐hydroxyglurate directly induces vasculogenic mimicry through PHLDB2 in renal cell carcinoma

Metabolism reprograming is a hallmark of cancer and plays an important role in tumor progression. The aberrant metabolism in renal cell carcinoma (RCC) leads to accumulation of the oncometabolite L‐2‐hydroxyglurate (L‐2HG). L‐2HG has been reported to inhibit the activity of some α‐ketoglutarate‐dependent dioxygenases such as TET enzymes, which mediate epigenetic alteration, including DNA and histone demethylation. However, the detailed functions of L‐2HG in renal cell carcinoma have not been investigated thoroughly. In our study, we found that L‐2HG was significantly elevated in tumor tissues compared to adjacent tissues. Furthermore, we demonstrated that L‐2HG promoted vasculogenic mimicry (VM) in renal cancer cell lines through reducing the expression of PHLDB2. A mechanism study revealed that activation of the ERK1/2 pathway was involved in L‐2HG‐induced VM formation. In conclusion, these findings highlighted the pathogenic link between L‐2HG and VM and suggested a novel therapeutic target for RCC.
Dual Role of the PTPN13 Tyrosine Phosphatase in Cancer

In this review article, we present the current knowledge on PTPN13, a class I non-receptor protein tyrosine phosphatase identified in 1994. We focus particularly on its role in cancer, where PTPN13 acts as an oncogenic protein and also a tumor suppressor. To try to understand these apparent contradictory functions, we discuss PTPN13 implication in the FAS and oncogenic tyrosine kinase signaling pathways and in the associated biological activities, as well as its post-transcriptional and epigenetic regulation. Then, we describe PTPN13 clinical significance as a prognostic marker in different cancer types and its impact on anti-cancer treatment sensitivity. Finally, we present future research axes following recent findings on its role in cell junction regulation that implicate PTPN13 in cell death and cell migration, two major hallmarks of tumor formation and progression.
What Can Neuromarketing Tell Us about Food Packaging?

Packaging is a powerful tool for brands, which can not only catch consumers’ attention but also influence their purchase decisions. The application of neuromarketing techniques to the study of food packaging has recently gained considerable popularity both in academia and practice, but there are still some concerns about the methods and metrics commercially offered and the interpretation of their findings. This represents the motivation of this investigation, whose objective is twofold: (1) to analyze the methodologies and measurements commonly used in neuromarketing commercial research on packaging, and (2) to examine the extent to which the results of food packaging studies applying neuromarketing techniques can be reproduced under similar methodologies. Obtained results shed light on the application of neuromarketing techniques in the evaluation of food packaging and reveal that neuromarketing and declarative methodologies are complementary, and its combination may strengthen the studies’ results. Additionally, this study highlights the importance of having a framework that improves the validity and reliability of neuromarketing studies to eradicate mistrust toward the discipline and provide brands with valuable insights into food packing design.
Object shape affects hand grip function for heavy objects in younger and older adults

Abstract Hand-grip function while lifting objects is essential for performing everyday tasks. The Box and Block Test (BBT) has been used to assess hand-grip function and dexterity, but only light objects have been used. The purpose of this study was to investigate the effects of block surface, shape, age, and sex on hand dexterity during the movement of heavy blocks in the BBT. Forty healthy participants comprised of a younger group of 10 males (M = 22.50 years, SD = 2.01) and 10 females (M = 22.20 years, SD = 2.66) between 20 and 30-years-old, and an older group of 10 males (M = 55.80 years, SD = 3.19) and 10 females (M = 55.90 years, SD = 2.56) between 50 and 60-years-old, performed the BBT using steel objects of different shapes (cylindrical and cubic) and different surfaces (plain steel, black spray-painted steel, and yellow non-slip spray-painted steel). The results indicated that repetition and shape had significant effects on the BBT score. Previous researchers have found that surface textures, age, and sex influenced hand dexterity for light objects, but these effects were not discovered in the current study. These findings suggest that shape should take priority over age, sex, and surface texture when designing tools to improve user handling. Practitioner Summary: Hand-grip function is critically important when performing daily grip tasks such as picking up a writing utensil, using a toothbrush, or twisting a hand tool. The shape should be considered during the design process of tools and equipment used in industry so that handling can be made for the user. Abbreviations: BBT: box and block test; COF: coefficient of friction; BPS: blocks per second
Post-Vaccination Yellow Fever Antiserum Reduces Zika Virus in Embryoid Bodies When Placental Cells are Present

Zika virus (ZIKV) is a flavivirus that originated in Africa but emerged in Latin America in 2015. In this region, other flaviviruses such as Dengue (DENV), West Nile, and Yellow Fever virus (YFV) also circulate, allowing for possible antigenic cross-reactivity to impact viral infections and immune responses. Studies have found antibody-mediated enhancement between DENV and ZIKV, but the impact of YFV antibodies on ZIKV infection has not been fully explored. ZIKV infections cause congenital syndromes, such as microcephaly, necessitating further research into ZIKV vertical transmission through the placental barrier. Recent advancements in biomedical engineering have generated co-culture methods that allow for the in vitro recapitulation of the maternal–fetal interface. This study utilized a transwell assay, which was a co-culture model utilizing human placental syncytiotrophoblasts, fetal umbilical cells, and a differentiating embryoid body, to replicate the maternal–fetal axis. To determine if cross-reactive YFV vaccine antibodies impacted the pathogenesis of ZIKV across the maternal–fetal axis, syncytiotrophoblasts were inoculated with ZIKV or ZIKV incubated with YFV vaccine antisera, and the viral load was measured 72 h post-inoculation. Here, we report that BeWo and HUVEC cells were permissive to ZIKV and that the impact of YFV post-vaccination antibodies on ZIKV replication was cell line-dependent. Embryoid bodies were also permissive to ZIKV, and the presence of YFV antibodies collected 4–14 months post-vaccination reduced ZIKV infection when placental cells were present. However, when directly infected with ZIKV, the embryoid bodies displayed significantly increased viral loads in the presence of YFV antiserum taken 30 days post-vaccination. The data show that each of the cell lines and EBs have a unique response to ZIKV complexed with post-vaccination serum, suggesting there may be cell-specific mechanisms that impact congenital ZIKV infections. Since ZIKV infections can cause severe congenital syndromes, it is crucial to understand any potential enhancement or protection offered from cross-reactive, post-vaccination antibodies.
Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor

ABSTRACT The present study aimed to assess the role of miR-1275 in cardiac ischemia reperfusion injury. H9 human embryonic stem cell (hESC)-derived cardiomyocytes stimulated by oxygen-glucose deprivation/reoxygenation (OGD/R) were used to simulate myocardial injury in vitro. miR-1275 expression levels in cells were measured by RT-qPCR. The release of lactate dehydrogenase (LDH) and creatine kinase (CK) was examined through LDH and CK ELISA kits. Cell apoptosis was detected through flow cytometry. A Fura-2 Calcium Flux Assay Kit and a Fluo-4 assay kit were used to determine the Ca2+ concentration. Expression levels of proteins were tested by Western blotting. The binding effect of miR-1275 and neuromedin U type 1 receptor (NMUR1) was detected by dual-luciferase activity assay. The results showed that miR-1275 was upregulated in OGD/R-stimulated cardiomyocytes. Inhibition of miR-1275 suppressed the increased activity of LDH and CK, cell apoptosis, reactive oxygen species (ROS) production, intracellular Ca2+ concentration and sarcoplasmic reticulum (SR) Ca2+ leak induced by OGD/R treatment in cardiomyocytes. miR-1275 directly targets 3ʹUTR of NMUR1 and negatively regulates NMUR1 expression. Silence of NMUR1 abolished the protecting effect of the miR-1275 antagomir on myocardial OGD/R injury. Our study indicated that the miR-1275 antagomir protects cardiomyocytes from OGD/R injury through the promotion of NMUR1.
Synthesis, Characterization and X-ray Structures of Diphenyltin(IV) N-(2-Hydroxyacetophenone)glycinate, Its 1:1 Adduct with Triphenyltin(IV) Chloride, and Related Systems

The compound [Ph2Sn(2-OC6H4C(CH3)NCH2COO)], 1, shows a distorted trigonal-bipyramidal geometry in the solid state. Reaction of 1 with Ph3SnCl yields a 1:1 adduct in which the two tin atoms are joined via the carbonyl atom of the ligand in 1 to form a mixed diorgano/triorgano species. NMR data indicate that the 1:1 adduct dissociates in noncoordinating solvent. Similarly, [tBu2Sn(2-OC6H4C(CH3)NCH2COO)·tBu2SnCl2], which has been characterized crystallographically in the solid state, is dissociated in solution. In contrast to the above behavior, monomeric [Vin2Sn(2-OC6H4C(CH3)NCH2COO)] forms an adduct with a water molecule to yield [Vin2Sn(2-OC6H4C(CH3)NCH2COO)OH2] in the solid state.
Effects of a Person Centered Dementia Training Program in Greek Hospital Staff—Implementation and Evaluation

People with Dementia (PwD) are frequently admitted in general hospitals. However, health care professionals have lack of dementia knowledge, negative attitudes toward dementia, and lack of confidence in caring those patients. The aim of this study is to develop, implement and evaluate a dementia staff training program in Greek general hospitals. It was a repeated-measures research design. Fourteen (14) two-day workshops were conducted, consisting of six targeted and interactive modules. Staff members (N = 242) attended the program and were assessed according to (1) individual performance: questionnaires about attitudes towards dementia, confidence in care, knowledge about dementia and anxiety before, immediately after the training and three months later, (2) an overall training evaluation immediately after the training and (3) an evaluation of training implementation three months later. Positive attitudes towards dementia, improvement of confidence in care and decrease of feeling of anxiety as a trait, were sustained over time. Knowledge about dementia also increased after the training and sustained, with, however, a slight decrease over time. A well applied training program seems to provide the basis of a better care in PwD during hospitalization. However, changes in the organizational level and a transformation of care culture are necessary for training sustainability over time.
Effect of Spent Mushroom (Cordyceps militaris) on Growth Performance, Immunity, and Intestinal Microflora in Weaning Pigs

Simple Summary There are limited published reports regarding the optimal dosage of spent mushroom (Cordyceps militaris) as an antibiotic substitute in weaning pigs. This study investigated the effect of various dosages of the spent mushroom in-feed supplement on the growth performance, immunity, inflammation, and microbial count of weaning pigs. The supplementation at a level of 1.5 g/kg spent mushroom improved body weight, average daily gain, average daily feed intake, immunoglobulin A concentration, and Lactobacillus spp. , whereas it decreased blood cholesterol, interleukin-1, tumor necrosis factor-α, and Escherichia coli. This approach provides a basis for the possible use of C. militaris spent mushroom as an alternative growth promoter in weaned pigs. Abstract There are limited published reports regarding the optimal dosage of spent mushroom. This study investigated the effect of various levels of spent mushroom derived from C. militaris as an alternative growth promoter to an in-feed antibiotic on the growth performance, blood profiles, immunoglobulin, inflammation, and microbial count of weaning pigs. A total of 120 pigs (6.63 ± 0.13 kg initial body weight) were blocked by weight and sex in a randomized complete block design. Each treatment had six replicates of four pigs each. The pigs were allotted into five treatments: (1) positive control (PC) with 150 mg/kg colistin; (2) negative control (NC) without antibiotic inclusion; and (3–5) negative control groups with 0.5, 1.0, and 1.5 g/kg of C. militariss pent mushroom (SM), respectively. Blood samples were collected at day 35 for determination of blood-related lipid metabolism and immunity. Fresh fecal samples were collected to examine microbial counts on day 35 postweaning. The results showed that SM at 1.5 g/kg improved the body weight, average daily weight gain, and average daily feed intake of weaning pigs in the overall period (p < 0.05). Moreover, the highest dosage of SM caused improvements in the concentrations of high-density lipoprotein, and immunoglobulin A, along with suppressions of total cholesterol, interleukin-1, tumor necrosis factor-α, and E. coli (p < 0.05). Therefore, the weaned pigs fed a 1.5 g/kg SM diet showed improved growth performance and displayed greater immunoglobulin secretion and lower inflammation, pathogenic population, and cholesterol concentration.
The Use of Antimicrobials in Italian Heavy Pig Fattening Farms

Data on antimicrobial use (AMU) in heavy pig production (>150 kg) are limited. The aim of this study was to investigate the AMU in this production. Data from 2015 were collected for 143 fattening farms. The AMU was estimated through a treatment index per 100 days (TI100) using the defined daily dose animal for Italy (DDDAit). When possible, a comparison with the European Medicines Agency’s defined daily doses for animals (DDDvet) was performed. The median TI100 was 10.7 (range, 0.2–49.5). Group treatments represented 94.6% of overall consumption. The AMU calculated using DDDAit and DDDvet were strongly correlated (ρ = 0.976; p < 0.001). The AMU was negatively correlated with injectables use (ρ = −0.46, p < 0.001) and positively correlated with oral products (ρ = 0.21, p = 0.014), premixes (ρ = 0.26, p = 0.002), and mortality (ρ = 0.18; p = 0.027). Farm size was negatively correlated with AMU (ρ = −0.29, p < 0.001). Smaller farms were more frequently above the median TI100 (odds ratio = 2.3, 95% confidence interval = 1.2–4.7), suggesting that they may have lower biosecurity and management standards. The results of this study should provide useful insights for the development of an Italian monitoring system.
Acarbose bioequivalence: Exploration of eligible protocol design

Acarbose is a poorly absorbed α‐glucosidase inhibitor that acts locally in the intestinal tract. Therefore, the evaluation of its bioequivalence (BE) should be based on pharmacodynamic (PD) rather than pharmacokinetic (PK) endpoints. Currently, there is no consensus on the best method for acarbose BE evaluation. The optimal protocol design regarding dosing time/dose and PD parameters requires further exploration. The aim of the study was to identify an optimum protocol for establishing acarbose BE in healthy Chinese volunteers using PD endpoints.
EEG-Based Estimation on the Reduction of Negative Emotions for Illustrated Surgical Images

Electroencephalogram (EEG) biosignals are widely used to measure human emotional reactions. The recent progress of deep learning-based classification models has improved the accuracy of emotion recognition in EEG signals. We apply a deep learning-based emotion recognition model from EEG biosignals to prove that illustrated surgical images reduce the negative emotional reactions that the photographic surgical images generate. The strong negative emotional reactions caused by surgical images, which show the internal structure of the human body (including blood, flesh, muscle, fatty tissue, and bone) act as an obstacle in explaining the images to patients or communicating with the images with non-professional people. We claim that the negative emotional reactions generated by illustrated surgical images are less severe than those caused by raw surgical images. To demonstrate the difference in emotional reaction, we produce several illustrated surgical images from photographs and measure the emotional reactions they engender using EEG biosignals; a deep learning-based emotion recognition model is applied to extract emotional reactions. Through this experiment, we show that the negative emotional reactions associated with photographic surgical images are much higher than those caused by illustrated versions of identical images. We further execute a self-assessed user survey to prove that the emotions recognized from EEG signals effectively represent user-annotated emotions.
Subthalamic Stimulation for Camptocormia in Parkinson’s Disease: Association of Volume of Tissue Activated and Structural Connectivity with Clinical Effectiveness

Background: Deep brain stimulation of the subthalamic nucleus (STN-DBS) has been reported to be effective for camptocormia in Parkinson’s disease (PD). However, the association between clinical effectiveness and the stimulated volumes or structural connectivity remains unexplored. Objective: To investigate the effectiveness of STN-DBS for treating camptocormia in PD and its association with volumes of tissue activated (VTA) and structural connectivity. Methods: We reviewed video recordings of patients who had undergone STN-DBS. The total and upper camptocormia (TCC and UCC) angles were measured to quantify changes in camptocormia. The Movement Disorders Society Unified Parkinson’s Disease Rating Scale III (MDS-UPDRS III) was used to assess motor symptoms. Pre- and postoperative brain images were collected for modeling volume of VTA and structural connectivity using Lead-DBS software. Results: Participants included 36 patients with PD (8 with TCC-camptocormia and 2 with UCC-camptocormia) treated with bilateral STN-DBS. After surgery, patients showed a significant improvement in postural alignment at follow-up (mean follow-up duration: 6.0±2.2 months). In the entire sample, higher structural connectivity to the right supplementary motor area (SMA) and right lateral premotor cortex along the dorsal plane (PMd) was associated with larger postsurgical improvements in axial signs and TCC angles after stimulation was turned on. In patients diagnosed with camptocormia, larger improvement in camptocormia angles after STN-DBS was associated with a larger VTA overlap with STN (R = 0.75, p = 0.032). Conclusion: This study suggests that both VTA overlap with STN and structural connectivity to cortical motor regions are associated with the effectiveness of STN-DBS for managing camptocormia in PD.
Protein absorption and kinetics in critical illness

Purpose of review Timing, dose, and route of protein feeding in critically ill patients treated in an ICU is controversial. This is because of conflicting outcomes observed in randomized controlled trials (RCTs). This inconsistency between RCTs may occur as the physiology of protein metabolism and protein handling in the critically ill is substantially different from the healthy with limited mechanistic data to inform design of RCTs. This review will outline the current knowledge and gaps in the understanding of protein absorption and kinetics during critical illness. Recent findings Critically ill patients, both children and adults, lose muscle protein because of substantial increases in protein degradation with initially normal, and over time increasing, protein synthesis rates. Critically ill patients appear to retain the capacity to absorb dietary protein and to use it for building body protein; however, the extent and possible benefit of this needs to be elucidated. More sophisticated methods to study protein absorption and digestion have recently been described but these have yet to be used in the critically ill. Summary Adequate understanding of protein absorption and kinetics during critical illness will help the design of better interventional studies in the future. Because of the complexity of measuring protein absorption and kinetics in the critically ill, very few investigations are executed. Recent data using isotope-labelled amino acids suggests that critically ill patients are able to absorb enteral protein and to synthesize new body protein. However, the magnitude of absorption and anabolism that occurs, and possible benefits for the patients need to be elucidated.
In Vitro Degradation of Plasticized PLA Electrospun Fiber Mats: Morphological, Thermal and Crystalline Evolution

In the present work, fiber mats of poly(lactic acid), PLA, plasticized by different amounts of oligomer lactic acid, OLA, were obtained by electrospinning in order to investigate their long term hydrolytic degradation. This was performed in a simulated body fluid for up to 352 days, until the complete degradation of the samples is reached. The evolution of the plasticized electrospun mats was followed in terms of morphological, thermal, chemical and crystalline changes. Mass variation and water uptake of PLA-based electrospun mats, together with pH stability of the immersion media, were also studied during the in vitro test. The results showed that the addition of OLA increases the hydrolytic degradation rate of PLA electrospun fiber mats. Moreover, by adding different amounts of OLA, the time of degradation of the electrospun fiber mats can be modulated over the course of a year. Effectively, by increasing the amount of OLA, the diameter of the electrospun fibers decreases more rapidly during degradation. On the other hand, the degree of crystallinity and the dimension of the α crystals of the electrospun fiber mats are highly affected not only by the presence but also by the amount of OLA during the whole process.
Influence of Sandblasting Particle Size and Pressure on Resin Bonding Durability to Zirconia: A Residual Stress Study

The influence of residual stress induced by sandblasting the zirconia ceramic surface on the resin bonding to the ceramic is still unclear. The effect of four different sandblasting conditions (with 50 and 110 μm alumina at pressures of 0.2 and 0.4 MPa) on the bonding of adhesive resin cement (Panavia F 2.0) to zirconia (Cercon® ht) was investigated in terms of residual stress. The surface roughness and water contact angle of the zirconia surfaces were measured. The tetragonal-to-monoclinic (t–m) phase transformation and residual stresses (sin2ψ method) were studied by X-ray diffraction. The resin-bonded zirconia specimens were subjected to shear bond strength (SBS) tests before and after thermocycling (10,000 and 30,000 cycles) (n = 10). As the particle size and pressure increased, the roughness gradually and significantly increased (p = 0.023). However, there were no significant differences in roughness-corrected contact angle among all the sandblasted groups (p > 0.05). As the particle size and pressure increased, the m-phase/(t-phase + m-phase) ratios and compressive residual stresses gradually increased. After thermocycling, there were no significant differences in SBS among the sandblasted zirconia groups (p > 0.05). In conclusion, increased surface roughness and residual stress do not directly affect the resin bonding durability.