Miracle-dz/UniFAR-FTUs huggingface dataset:
Gastric dysfunction in patients with chronic nausea and vomiting syndromes defined by a novel non-invasive gastric mapping device

Importance Chronic nausea and vomiting syndromes (NVS) are prevalent and debilitating disorders. Putative mechanisms include gastric neuromuscular disease and dysregulation of brain-gut interaction, but clinical tests for objectively defining gastric motor function are lacking. Objective A novel medical device enabling non-invasive body surface gastric mapping (BSGM) was developed and applied to evaluate NVS pathophysiology. Design A case-control study where BSGM was performed in NVS patients and matched controls using Gastric Alimetry (Alimetry, New Zealand), comprising a conformable high-resolution array (8x8 electrodes; 20 mm inter-electrode spacing), wearable Reader, and validated symptom logging App. Continuous measurement encompassed a fasting baseline (30 min), 482 kCal meal (10 min), and 4-hr post-prandial recording. Setting Multicenter study in Auckland, New Zealand and Calgary, Canada. Participants 43 NVS patients (gastroparesis and Rome IV chronic NVS) and 43 matched controls. Main outcomes and measures Symptom severity and quality of life were measured using Patient Assessment of Upper Gastrointestinal Disorders-Symptom Severity Index (PAGI-SYM), Gastroparesis Cardinal Symptom Index (GCSI), and Patient Assessment of Upper Gastrointestinal Disorders-Quality of Life (PAGI-QOL) instruments. Health psychology metrics included the State Trait Anxiety Inventory Short Form (STAI-SF) and Patient Health Questionnaire-2 (PHQ-2) questionnaires. Spectral analyses including frequency, amplitude, and fed-fasting power ratio. Spatial biomarker analyses included spatial frequency stability and average spatial covariance. Results Meal responses were impaired in NVS, with reduced amplitudes compared to controls (median 23.3 vs 38.0 microV, p<0.001), impaired fed-fasting power-ratios (1.1 vs 1.6, p=0.02), and disorganized slow-waves (spatial frequency stability 13.6 vs 49.5; p<0.001). However, two distinct NVS subgroups were evident with indistinguishable symptoms (all p>0.05). A majority (62%) had normal BSGM studies (all biomarkers non-significant vs controls) with increased psychological comorbidities (43.5% vs 7.7%; p=0.03) and anxiety scores (median 16.5 vs 13.0; p=0.035). A smaller subgroup (31%) had markedly abnormal BSGM, with test biomarkers correlating with symptoms (nausea, pain, excessive fullness, early satiety, bloating; all r>0.35, p<0.05). Conclusions and Relevance NVS patients share overlapping symptoms, but comprise distinct underlying phenotypes as revealed by a novel BSGM device. These phenotypes correlate with symptoms, which should inform clinical management and allocations into therapeutic trials.
Increased household transmission and immune escape of the SARS-CoV-2 Omicron variant compared to the Delta variant: evidence from Norwegian contact tracing and vaccination data

We compared the secondary attack rate (SAR) of the SARS-CoV-2 Omicron and Delta variants in households using contact tracing data. Omicron SAR was higher (41%) than Delta (35%), likely due to immune evasion. Booster dose reduces risk of infection but has limited effect on preventing Omicron transmission.
A Bibliometric Analysis of Ophthalmology Resident Research Productivity in the United States

Objective: The extent and impact of ophthalmology resident scholarly output is not well known. The authors aim to quantify scholarly activity of ophthalmology residents during residency and assess what factors may be associated with greater research productivity of these residents. Methods: Ophthalmology residents who graduated in 2021 were identified from their respective program websites. Bibliometric data published by these residents between the beginning of their PGY-2 year (July 1st, 2018) until three months after graduation (September 30th, 2021) were captured through searches via PubMed, Scopus, and Google Scholar. The association of the following factors with greater research productivity numbers was analyzed: residency tier, medical school rank, sex, doctorate degree, type of medical degree, and international medical graduate status. Residency programs were sorted into five tiers by Doximity "reputation" and medical schools were stratified into US News Top 40 and non-Top 40 schools. Results: We found 418 ophthalmology residents from 98 residency programs. These residents published a mean (+/- SD) number of 2.68 +/- 3.81 peer-reviewed publications each; the number of ophthalmology-related articles published was 2.39 +/- 3.40 and the number of first author publications was 1.18 +/- 1.96. The mean (+/- SD) h -index for this cohort of ophthalmology residents was 0.79 +/- 1.17. Upon multivariate analysis, we discovered significant correlations between both residency tier and medical school rank and all bibliometric variables assessed. Pairwise comparisons revealed that residents from higher tier programs had greater research productivity numbers than those from lower tier programs. Conclusions: We obtained bibliometric standards for ophthalmology residents on a national scale. Residents who graduated from higher-ranked residency programs and medical schools possessed higher h -indices and published more peer-reviewed publications, ophthalmology-related articles, and first author publications.
Effects of heavy metals on bacterial community surrounding Bijiashan mining area located in northwest China

Abstract Heavy metal (HM) pollution is a severe and common environmental problem in mining area soil. It is imperative to understand the micro ecological characteristics of mining area soil for HM contaminated soil remediation. This study described the effects of HM pollution level and soil physical and chemical parameters on microbial diversity. In this study, high-throughput sequencing technology was used to study the effects of HM pollution on the diversity and composition of the soil microbial community. The soil groups were barren, exhibiting alkaline pH, low total nitrogen (TN), and total potassium (TK) according to soil fertility standard. Compared with the control group, there was severe multiple HM pollution in the other five groups, including lead (Pb), cadmium (Cd), zinc (Zn), and copper (Cu). The dominant phyla accounting for more than 1% of the overall community in all soil groups were Proteobacteria (34.432 ± 7.478%), Actinobacteria (22.947 ± 4.297%), Acidobacteria (10.47 ± 2.439%), Chloroflexi (7.89 ± 2.980%), Planctomycetota (5.993 ± 1.558%), Bacteroidota (4.275 ± 1.980%), Cyanobacteria (3.478 ± 2.196%), Myxococcus (2.888 ± 0.822%), Gemmatimonadota (2.448 ± 0.447%), Firmicutes (1.193 ± 0.634%), Patescibacteria (0.435 ± 0.813%), and Nitrospirota (0.612 ± 0.468%). Proteobacteria and Actinobacteria were predominant at the phylum level, which showed a certain tolerance to HMs. In addition, redundancy analysis (RDA) results showed that Pb, Cu, Zn, and Cd were strongly correlated with each other (P < 0.01). Other nutrient elements (except for TK) were significantly positively correlated with each other. Cu and nutrient element TK had an important impact on bacterial community structure. Therefore, bacteria with the function of HM tolerance and bioremediation in extreme environments should be researched, which provides a foundation for future ecological remediation of contaminated soil by using microbial remediation technology. Graphical abstract
EEG Based Artistic Visualization of Dreams

The purpose of this research is to explore the artistic visualization of dreams based on human electroencephalogram(EEG) data during REM sleep, and the generation of analytic dream-related images through a computer algorithm. This study utilized a polysomnography EEG monitoring device to keep track of the sleep activities of 11 subjects, primarily extracting objective data from the dreaming stage. Subjective measurement such as psychological questionnaire was also applied to assist in emotion evaluation to eventually generate an abstract visual expression of dreams. The study discusses a method of creating visual images of dreams based on objective EEG data signals combined with subjective emotion evaluation, providing a new path for the explicit visual expression of implicit conscious activities.
Client layer becomes bottleneck: workload analysis of an ultra-large-scale cloud storage system

Recent years have witnessed the fast development of file and storage systems. Many improvements of file and storage systems are inspired by Workload analysis, which reveals the characteristics of I/O behavior. Although cloud storage systems are becoming increasingly prominent, few real-world and large-scale cloud storage workload studies are presented. Alibaba Cloud is one of the world's largest cloud providers, and we have collected and analyzed workloads from Alibaba for an extended period. We observe that modern cloud network architecture can easily handle the peak load during busy festivals. However, the client layer is the system bottleneck during the peak period, which calls for further optimization. We also find that the workload is heavily skewed toward a small percentage of virtual disks, and its distribution conforms 80/20 rule. In summary, the characteristics of such a large-scale cloud storage system in production environments are important for future cloud storage system modifications.
Peripheral Display for Conveying Real-time Pain Condition of Persons with Severe Intellectual Disabilities to their Caregivers

An effective assisting tool for caregivers to monitor pain of persons with severe intellectual disabilities (SID) is eagerly needed, since these persons have difficulties with self-report. The Bio response System detecting stress and the techniques with potential to distinguish pain from stress suggest the possibility to detect pain with physiological data. In the current paper, we propose the design of a peripheral display for making caregivers aware of the real-time pain condition of their clients without added attention burden. An iterative user-centered design process resulted in two prototypes and corresponding evaluations of one peripheral display. The potential of the display to help caregivers be promptly aware of the pain condition of clients was tested with targeted users. Guidelines for the design of peripheral display applications in similar semi-hospitalized contexts are provided. Further steps in this study will be to test the adjusted Bio response System for detecting pain in persons with SID adequately.
Venetoclax combined with FLAG‐based chemotherapy induces an early and deep response in mixed‐phenotype‐acute leukemia

REFERENCES 1. Singh A, Brandow AM, Panepinto JA. COVID-19 in individuals with sickle cell disease/trait compared with other Black individuals. Blood Adv. 2021;5:1915-1921. 2. Arlet JB, de Luna G, Khimoud D, et al. Prognosis of patients with sickle cell disease and COVID-19: a French experience. Lancet Haematol. 2020;7:e632-e634. 3. Panepinto JA, Brandow A, Mucalo L, et al. Coronavirus disease among persons with sickle cell disease, United States, March 20–May 21, 2020. Emerg Infect Dis. 2020;26:2473-2476. 4. Telfer P, De la Fuente J, Sohal M, et al. Real-time national survey of COVID-19 in hemoglobinopathy and rare inherited anemia patients. Haematologica. 2020;105:2651-2654. 5. Elenga N, Celicourt D, Muanza B, et al. Dengue in hospitalized children with sickle cell disease: a retrospective cohort study in the French departments of America. J Infect Public Health. 2020;13: 186-192. 6. Rankine-Mullings A, Reid ME, Moo Sang M, Richards-Dawson MA, Knight-Madden JM. A retrospective analysis of the significance of haemoglobin SS and SC in disease outcome in patients with sickle cell disease and dengue fever. EBioMedicine. 2015;2:937-941. 7. Renoux C, Romana M, Joly P, et al. Effect of age on blood rheology in sickle cell anaemia and sickle cell Haemoglobin C disease: a crosssectional study. PLoS One. 2016;11:e0158182.
An Experimental Analysis of the Real Dynamic Behavior and the Reliability Verification of an Existing Footbridge

The investigated existing footbridge, on which the dynamic experimental analysis was concentrated, was built across Vltava River in Prague in 1984. Since putting into operation, the footbridge was apparently sensitive to dynamic excitation by passing pedestrians. Above that in the course of its existence the considerable part of the footbridge deck was overflowed by two great floods that happened in August 2002 and in June 2013. After the second flood, a visible damage on the footbridge deck was detected by a visual inspection. The described dynamic experiment was divided into two basic parts. The experimental modal analysis was carried out first and then the dynamic load test was realized. It was focused to the deck vibrations induced by different pedestrians groups. It was found out the footbridge deck vertical accelerations exceed the pedestrian comfort limit in some normal situations. On the basis of comparison between modes of natural vibrations, which were evaluated by the experimental modal analysis and the equivalent ones from previous dynamic experiments, it was validated that the discovered deck damage caused the partial variation of fundamental dynamic footbridge behavior.
Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate

Trafficking regulator of GLUT4-1, TRARG1, positively regulates insulin-stimulated GLUT4 trafficking and insulin sensitivity. However, the mechanism(s) by which this occurs remain(s) unclear. Using biochemical and mass spectrometry analyses we found that TRARG1 is dephosphorylated in response to insulin in a PI3K/Akt-dependent manner and is a novel substrate for GSK3. Priming phosphorylation of murine TRARG1 at serine 84 allows for GSK3-directed phosphorylation at serines 72, 76 and 80. A similar pattern of phosphorylation was observed in human TRARG1, suggesting that our findings are translatable to human TRARG1. Pharmacological inhibition of GSK3 increased cell surface GLUT4 in cells stimulated with a submaximal insulin dose, and this was impaired following Trarg1 knockdown, suggesting that TRARG1 acts as a GSK3-mediated regulator in GLUT4 trafficking. These data place TRARG1 within the insulin signaling network and provide insights into how GSK3 regulates GLUT4 trafficking in adipocytes.
ATP synthase is essential for high conductance but not low conductance Mitochondrial Permeability Transition

A sudden increase in permeability of the mitochondrial inner membrane, known as mitochondrial permeability transition (PT), is the central event responsible for cell death and tissue damage in conditions such as stroke and heart attack. PT is caused by the opening of the Cyclosporin A (CSA) dependent calcium-induced large pore, the Permeability Transition Pore (PTP). The molecular details of PTP opening remain incompletely understood. We utilized holographic microscopy imaging to directly assess PTP induction in HAP1 living cells. We found that calcium stress caused CSA-sensitive depolarization of the mitochondrial inner membrane. PTP did not occur until after nearly complete mitochondrial membrane depolarization. In cells lacking c and δ subunits of the ATP synthase, we observed CSA-sensitive membrane depolarization but not highconductance PTP. We propose that PTP activation occurs as a two-phase process, with the initial membrane depolarization followed by the second phase of large pore opening that requires ATP synthase.
Understanding “Bao Xi Bu Bao You” in the Sharing of Emotion-Associated Personal Events in Chinese Families from the Perspective of Adult Children

Abstract:“Bao Xi Bu Bao You” is a Chinese saying that means sharing information associated with positive emotions and withholding information associated with sadness or concern. This paper explores the decision-making practices of Chinese adults related to Bao Xi Bu Bao You in their sharing of current emotion-associated personal events with their parents. Based on semistructured interviews with thirty-two participants, the study illuminates the complexities of child-to-parent Bao Xi Bu Bao You activities by illustrating how they are shaped by decision-making processes that juggle several elements: expectations of reaction alignment/misalignment, utility, care, risk of boundary turbulence, and degree of seriousness/significance. Because the emotional quality of Xi (positive events) and You (sad/concerning events) are different, the elements are framed and approached in slightly different ways for decisions to share Xi and to withhold You, with some elements being particularly salient in certain contexts. Children balance self-oriented and parent-oriented considerations in decision making and actively adjust their personal events into “better-shared” or “better-avoided” Xi and You across time. Care, modesty, and Taoist dialectical passive-active transformations emerged as notable Chinese cultural elements that inform Bao Xi Bu Bao You and shed light on the rich cultural meanings attached to such practices.
Identification of UBE2I as a Novel Biomarker in ccRCC Based on a Large-Scale CRISPR-Cas9 Screening Database and Immunohistochemistry

Background: The genome-wide CRISPR-cas9 dropout screening has emerged as an outstanding approach for characterization of driver genes of tumor growth. The present study aims to investigate core genes related to clear cell renal cell carcinoma (ccRCC) cell viability by analyzing the CRISPR-cas9 screening database DepMap, which may provide a novel target in ccRCC therapy. Methods: Candidate genes related to ccRCC cell viability by CRISPR-cas9 screening from DepMap and genes differentially expressed between ccRCC tissues and normal tissues from TCGA were overlapped. Weighted gene coexpression network analysis, pathway enrichment analysis, and protein–protein interaction network analysis were applied for the overlapped genes. The least absolute shrinkage and selection operator (LASSO) regression was used to construct a signature to predict the overall survival (OS) of ccRCC patients and validated in the International Cancer Genome Consortium (ICGC) and E-MTAB-1980 database. Core protein expression was determined using immunohistochemistry in 40 cases of ccRCC patients. Results: A total of 485 essential genes in the DepMap database were identified and overlapped with differentially expressed genes in the TCGA database, which were enriched in the cell cycle pathway. A total of four genes, including UBE2I, NCAPG, NUP93, and TOP2A, were included in the gene signature based on LASSO regression. The high-risk score of ccRCC patients showed worse OS compared with these low-risk patients in the ICGC and E-MTAB-1980 validation cohort. UBE2I was screened out as a key gene. The immunohistochemistry indicated UBE2I protein was highly expressed in ccRCC tissues, and a high-level nuclear translocation of UBE2I occurs in ccRCC. Based on the area under the curve (AUC) values, nuclear UBE2I had the best diagnostic power (AUC = 1). Meanwhile, the knockdown of UBE2I can inhibit the proliferation of ccRCC cells. Conclusion: UBE2I, identified by CRISPR-cas9 screening, was a core gene-regulating ccRCC cell viability, which accumulated in the nucleus and acted as a potential novel promising diagnostic biomarker for ccRCC patients. Blocking the nuclear translocation of UBE2I may have potential therapeutic value with ccRCC patients.
Changes in Gene Expression in Leaves of Cacao Genotypes Resistant and Susceptible to Phytophthora palmivora Infection

Black pod rot, caused by Phytophthora palmivora, is a devastating disease of Theobroma cacao L. (cacao) leading to huge losses for farmers and limiting chocolate industry supplies. To understand resistance responses of cacao leaves to P. palmivora, Stage 2 leaves of genotypes Imperial College Selection 1 (ICS1), Colección Castro Naranjal 51 (CCN51), and Pound7 were inoculated with zoospores and monitored for symptoms up to 48 h. Pound7 consistently showed less necrosis than ICS1 and CCN51 48 h after inoculation. RNA-Seq was carried out on samples 24 h post inoculation. A total of 24,672 expressed cacao genes were identified, and 2,521 transcripts showed induction in at least one P. palmivora-treated genotype compared to controls. There were 115 genes induced in the P. palmivora-treated samples in all three genotypes. Many of the differentially expressed genes were components of KEGG pathways important in plant defense signal perception (the plant MAPK signaling pathway, plant hormone signal transduction, and plant pathogen interactions), and plant defense metabolite biosynthesis (phenylpropanoid biosynthesis, α-linolenic acid metabolism, ethylene biosynthesis, and terpenoid backbone biosynthesis). A search of putative cacao resistance genes within the cacao transcriptome identified 89 genes with prominent leucine-rich repeat (LRR) domains, 170 protein kinases encoding genes, 210 genes with prominent NB-ARC domains, 305 lectin-related genes, and 97 cysteine-rich RK genes. We further analyzed the cacao leaf transcriptome in detail focusing on gene families-encoding proteins important in signal transduction (MAP kinases and transcription factors) and direct plant defense (Germin-like, ubiquitin-associated, lectin-related, pathogenesis-related, glutathione-S-transferases, and proteases). There was a massive reprogramming of defense gene processes in susceptible cacao leaf tissue after infection, which was restricted in the resistant genotype Pound7. Most genes induced in Pound7 were induced in ICS1/CCN51. The level of induction was not always proportional to the infection level, raising the possibility that genes are responding to infection more strongly in Pound7. There were also defense-associated genes constitutively differentially expressed at higher levels in specific genotypes, possibly providing a prepositioned defense. Many of the defense genes occur in blocks where members are constitutively expressed at different levels, and some members are induced by Ppal infection. With further study, the identified candidate genes and gene blocks may be useful as markers for breeding disease-resistant cacao genotypes against P. palmivora.
Changes in Physicochemical Properties, Volatile Profiles, and Antioxidant Activities of Black Apple During High-Temperature Fermentation Processing

Black apple is a new elaborated product obtained from whole fresh apple through fermentation at controlled high temperature (60~90°C) and humidity (relative humidity of 50~90%). The appearance, color, texture, and taste of black apple changed dramatically compared with those of fresh apple. In this study, changes in the physicochemical and phytochemical properties, volatile profiles, and antioxidant capacity of apple during the fermentation process were investigated. Results showed that the browning intensity and color difference increased continuously during the whole 65-day fermentation process (p < 0.05). Sugars decreased in the whole fermentation process (p < 0.05), whereas the contents of organic acids increased first and then decreased with prolonged 35 days of fermentation (p < 0.05). Total polyphenol content of black apple showed an increase of 1.5-fold as that of fresh apple, whereas 12 common polyphenolic compounds present in fresh apple decreased dramatically in the whole fermentation process (p < 0.05). The analysis of flavor volatiles showed that high-temperature fermentation decreased the levels of alcohols and esters and resulted in the formation of furanic and pyranic compounds, which are the main products of Maillard reaction (MR). Antioxidant activities of black apple were enhanced compared with those of fresh apple, and results indicated that the enhancement of antioxidant activities was related to the polyphenols and products of MR.