Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcription factors and transporters in zinc homeostasis: lessons learned from fungi

Abstract Zinc is an essential nutrient for all organisms because this metal serves as a critical structural or catalytic cofactor for many proteins. These zinc-dependent proteins are abundant in the cytosol as well as within organelles of eukaryotic cells such as the nucleus, mitochondria, endoplasmic reticulum, Golgi, and storage compartments such as the fungal vacuole. Therefore, cells need zinc transporters so that they can efficiently take up the metal and move it around within cells. In addition, because zinc levels in the environment can vary drastically, the activity of many of these transporters and other components of zinc homeostasis is regulated at the level of transcription by zinc-responsive transcription factors. Mechanisms of post-transcriptional control are also important for zinc homeostasis. In this review, the focus will be on our current knowledge of zinc transporters and their regulation by zinc-responsive transcription factors and other mechanisms in fungi because these organisms have served as useful paradigms of zinc homeostasis in all organisms. With this foundation, extension to other organisms will be made where warranted.
C-FGF23 peptide alleviates hypoferremia during acute inflammation

Hypoferremia results as an acute phase response to infection and inflammation aiming to reduce iron availability to pathogens. Activation of toll-like receptors (TLR), the key sensors of the innate immune system, induces hypoferremia mainly through the rise of the iron hormone hepcidin. Conversely, stimulation of erythropoiesis suppresses hepcidin expression via induction of the erythropoietin-responsive hormone erythroferrone. Iron deficiency stimulates transcription of the osteocyte- secreted protein FGF23. Here we hypothesized that induction of FGF23 in response to TLR4 activation is a potent contributor to hypoferremia and, thus, impairment of its activity may alleviate hypoferremia induced by lipopolysaccharide (LPS), a TLR 4 agonist. We used the C-terminal tail of FGF23 to impair endogenous full-length FGF23 signaling in wildtype mice, and investigated its impact on hypoferremia. Our data show that FGF23 is induced as early as pro-inflammatory cytokines in response to LPS, followed by upregulation of hepcidin and downregulation of erythropoietin (Epo) expression in addition to decreased serum iron and transferrin saturation. Further, LPS-induced hepatic and circulating hepcidin were significantly reduced by FGF23 signaling disruption. Accordingly, iron sequestration in liver and spleen caused by TLR4 activation was completely abrogated by FGF23 signaling inhibition, resulting in alleviation of serum iron and transferrin saturation deficit. Taken together, our studies highlight for the first time that inhibition of FGF23 signaling alleviates LPS-induced acute hypoferremia.
The Impact of Advertisement on Consumer Buying Behavior in Electronic Industry

The study examines the impact of advertisement on consumer buying behavior in electronic industry (consumer electronic products) within the metro cities of Maharashtra, India. Every company in India has a very aggressive attitude in advertising their products and services through effective promotional channel, this is because of huge competition which exists in the market. The want of advertising is vital for every company as it enables to promote their product and services and to influence the consumer buying decision. With the increase in technology, the companies are at a benefit to create more effective advertisement which has become extremely important for them to have a competitive advantage. This particular research was conducted on 150 male and female of all age types who use different brands of electronic products to check the influence of advertisements on their buying behavior while creating awareness and building the perceptions. Present research paper focuses on the impact of advertisement on consumer buying behavior under consumer electronic. This study adopted a survey research design. It involves assortment of knowledge from respondents through form to check hypotheses. This survey study was conducted on 7 December, 2019. A sample of 150 respondents from the cities of Mumbai and Pune was collected which were of various age groups. The study explores that a creative and well executed advertisement always has a great impact on the buying trends and the purchasing behavior of consumers in the electronic industry. At the same time the quality of product, price and brand also have a strong impact on the buying behavior of the consumer. Various different questions were asked to test the hypotheses and to obtain the best possible results.
Bioethanol Production from Carbohydrate-Enriched Biomass of Chlorella minutissima by extraction of carbohydrates through hydrolysis and fermentation technology using Saccharomyces cerevisiae 182

In the present study the potential of bioethanol production using carbohydrate-enriched biomass of the Chlorella minutissima was studied. The saccharification of the carbohydrate-enriched biomass, tow acids (H2SO4 and HNO3) was investigated. Each acid were used at three concentrations, 2.5 N, 1 N and 0.5 N and for each acid concentration the saccharification was conducted under three temperatures (40 °C, 60 °C and 100 °C). Higher acid concentrations gave in general higher reducing sugars (RS) yields (%, gRS/gTotal sugars) with higher rates, while the increase in temperature lead to higher rates at lower acid concentration. The hydrolysates then were used as substrate for ethanolic fermentation by Saccharomyces cerevisiae 182 strain. The bioethanol yield (%, gEtOH/gBiomass) was significantly affected by the acid concentration used for the saccharification of the carbohydrates. The highest bioethanol yields of 16.32% ± 0.98% (gEtOH/gBiomass) and 16.27% ± 0.95% (gEtOH/gBiomass) were obtained in hydrolysates produced with HNO3 0.5 N and H2SO4 1 N, respectively.
FORTRAN 77 Subroutines for the Solution of Skew-Hamiltonian/Hamiltonian Eigenproblems (cid:21) Part I: Algorithms and Applications 1

Skew-Hamiltonian/Hamiltonian matrix pencils λ S − H appear in many applications, including linear quadratic optimal control problems, H ∞ -optimization, certain multi-body systems and many other areas in applied mathematics, physics, and chemistry. In these applications it is necessary to compute certain eigenvalues and/or corresponding de(cid:29)ating subspaces of these matrix pencils. Recently developed methods exploit and preserve the skew-Hamiltonian/Hamiltonian structure and hence increase reliability, accuracy and performance of the computations. In this paper we describe the corresponding algorithms whose implementations have been included in the Subroutine Library in Control Theory (SLICOT). Furthermore we address some of their applications. We describe variants for real and complex problems with versions for factored and unfactored matrices S .
The effect of two different doses of dexmedetomidine to attenuate cardiovascular and airway responses to tracheal extubation: a double blind randomized controlled trial

Background: The objective of the study is to assess the effectiveness of two different doses of dexmedetomidine, an alpha 2 adrenergic agonist, to attenuate the cardiovascular and airway responses to tracheal extubation and to observe the adverse effects. Methodology: Ninety ASA grade I and II patients aged 18-50 years were randomized into three groups; A, B, and C to receive dexmedetomidine 0. 5µg/kg, 1µg/kg and normal saline placebo respectively about 15 minutes before discontinuation of inhalational agent. The heart rate, systolic blood pressure, diastolic blood pressure and mean arterial pressure were recorded during administration of drug, before extubation, during extubation, at 1, 3 minutes and every 5 minutes thereafter. Extubation quality was assessed on a 5 point scale and sedation by Ramsay sedation score. Results: There was significant decrease in heart rate and mean arterial pressure (p<0.001) during extubation in group A and B. Ninety percent of patients in group A, 93.3% patients in group B and 16.7% in group C could be extubated smoothly. The average time to extubate was 12.13±2.11, 14.08±3.19 and 10.27±2.09 minutes in groups A, B, and C respectively (P value <0.001). Higher incidence of bradycardia (p<0.001) was observed in Group A and B whereas incidence of breath holding was higher in group C (p=0.024). Conclusion: A dose of 0. 5µg/kg of dexmedetomidine administered as a bolus infusion before extubation attenuates the stress response to extubation as effectively as 1µg/kg. Higher sedation scores and longer time to extubate are seen with a dose of 1µg/kg without causing respiratory depression.
Efficacy of Combination Therapies on Neck Pain and Muscle Tenderness in Male Patients with Upper Trapezius Active Myofascial Trigger Points

Myofascial pain syndrome, thought to be the main cause of neck pain and shoulder muscle tenderness in the working population, is characterized by myofascial trigger points (MTrPs). This study aimed to examine the immediate and short-term effect of the combination of two therapeutic techniques for improving neck pain and muscle tenderness in male patients with upper trapezius active MTrPs. This study was a pretest-posttest single-blinded randomized controlled trial. Sixty male subjects with mechanical neck pain due to upper trapezius active MTrPs were recruited and randomly allocated into group A, which received muscle energy technique (MET) and ischemic compression technique (ICT) along with conventional intervention; group B, which received all the interventions of group A except ICT; and group C, which received conventional treatment only. Baseline (Pr), immediate postintervention (Po), and 2-week follow-up (Fo) measurements were made for all variables. Pain intensity and pressure pain threshold (PPT) were assessed by a visual analog scale (VAS) and pressure threshold meter, respectively. All the three groups received their defined intervention plans only. Repeated-measures analysis of variance was used to perform intra- and intergroup analyses. Cohen's d test was used to assess the effect size of the applied interventions within the groups. The intergroup analysis revealed significant differences among groups A, B, and C in VAS and PPT at Po (VAS-Po: F = 13.88, p=0.0001; PPT-Po: F = 17.17, p=0.0001) and even after 2 weeks of follow-up (VAS-Fo: F = 222.35, p=0.0001; PPT-Fo: F = 147.70, p=0.0001). Cohen's d revealed a significant treatment effect size within all groups except group C (only significant for VAS-Po–VAS-Pr: mean difference = 1.33, p < 0.05, d = 1.09); however, it showed a maximum effect size in group A for its variables (VAS-Fo–VAS-Pr: mean difference = 5.27, p=0.01, d = 4.04; PPT-Fo–PPT-Pr: mean difference = 2.14, p < 0.01, d = 3.89). Combination therapies (MET plus ICT) showed immediate and short-term (2-week follow-up) improvements in neck pain and muscle tenderness in male patients with upper trapezius active MTrPs.
Emotional Intelligent Workshop: Its Effect on Nursing Interns' Self-Confidence and Their Quality of Work Life

Background: Emotional intelligence is an important factor in achieving success in life including jobs and education. It affects not only success in the workplace and increase the quality of work life but it also, seems that emotions affect all our performance and abilities. Aim: The aim of the study was to investigate the effect of emotional intelligent workshop on nursing interns' self-confidence and their quality of work life. Method: A quasi-experimental research design with one-group pre-, post-, and follow-up test was used in conducting the present study. Sample: A simple random sample of 78 nursing interns was selected to be included in the study (7 males and 71 females) and have at least three months internship training period. Tool: The following tools were used: I:Emotional Intelligent Scale, II: Self-Confidence Scale, III: Quality of Work Life Scale. Results: the finding of the study indicated that there was low level of emotional intelligent, self confidence, and quality of work life among nursing interns at a pre-test phase. Also, there was statistically significant improvement in the nursing interns' emotional intelligent, self confidence, and quality of work life throughout the study phases (postand follow-up test). Furthermore, there was statistically significant positive correlation between emotional intelligent and self-confidence from one side and between emotional intelligent and quality of work life from another side among nursing interns. Conclusion: Emotional intelligent workshop has succeeded in achieving significant improvement in nursing interns' emotional intelligent, which was reflected on improvement of their self-confidence and quality of work life. Recommendation: Nursing Faculty should adopt non-traditional method of teaching as, problem based learning curriculum, which improve nursing students' emotional intelligent that on turn increase their self-confidence and their quality of work life.
Analysis of the thermal ratcheting phenomenon in packed-bed thermal energy storage using Discrete Element Method

Packed-bed thermal energy storages (TES) play a major role in energy technology. During energy absorption, hot air flows through the content of the TES in top-down direction. During the heating process, the expansion of the heat-storing medium (bulk material) leads to a stress increase on the walls of heat-storage tanks. These occurring loads are to be considered by means of a discretized model. Furthermore, it is of interest how the loads modify during several loading and unloading processes (thermal ratcheting phenomenon). In this paper, it will be investigated how this behaviour can be modelled using the dem approach.
Functional evaluation of bilateral arthroereisis of subtalar joint and simultaneous gastrocnemius lengthening in symptomatic flexible flatfoot in children

The aim of this study was to report the radiological and pedobarographic results of 10 children’s 20 feet with symptomatic flexible flat foot, who were treated with gastrocnemius lengthening with arthroereisis of subtalar joint. A total of 10 children’s 20 feet (5 boys and 5 girls between 9-14-year-old) with painful feet due to the flatfoot were treated in our clinic between August 2016 and December 2018. Bilateral gastrocnemius lengthening and arthroereisis of subtalar joint was performed simultaneously. Radiological evaluation was performed by measuring calcaneal pitch angle and Meary’s talusfirst metatarsal angles, pedobarographic evaluation by static and dynamic plantar heel and forefoot pressure data. The mean calcaneal pitch angle increased from 8° ± 0.93° preoperatively to 16.5° ± 1.14° postoperatively, and the mean Meary’s talus-first metatarsal angle decreased from 7.5° ± 1.14°preoperatively to 0.5° ± 0.51° postoperatively. The mean heel peak pressure and the forefoot peak pressure ascended from 11.5 ± 1.14 (N/cm2) and 10.5 ± 1.14 (N/cm2) preoperatively to 17.5 ± 1.14 (N/cm2) and 15.5 ± 1.14 (N/cm2) postoperatively, respectively. Clinical evaluation revealed that 9 patient had painful feet, while 1 patient had discomfort since the implant had been inserted.Arthroereisis of subtalar joint with simultaneous gastrocnemius lengthening provides painful feet and seems to be an effective, simple and safe solution for symptomatic flexible flatfoot in pediatric patients.
PGC-1α, Inflammation, and Oxidative Stress: An Integrative View in Metabolism

Peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α is a transcriptional coactivator described as a master regulator of mitochondrial biogenesis and function, including oxidative phosphorylation and reactive oxygen species detoxification. PGC-1α is highly expressed in tissues with high energy demands, and it is clearly associated with the pathogenesis of metabolic syndrome and its principal complications including obesity, type 2 diabetes mellitus, cardiovascular disease, and hepatic steatosis. We herein review the molecular pathways regulated by PGC-1α, which connect oxidative stress and mitochondrial metabolism with inflammatory response and metabolic syndrome. PGC-1α regulates the expression of mitochondrial antioxidant genes, including manganese superoxide dismutase, catalase, peroxiredoxin 3 and 5, uncoupling protein 2, thioredoxin 2, and thioredoxin reductase and thus prevents oxidative injury and mitochondrial dysfunction. Dysregulation of PGC-1α alters redox homeostasis in cells and exacerbates inflammatory response, which is commonly accompanied by metabolic disturbances. During inflammation, low levels of PGC-1α downregulate mitochondrial antioxidant gene expression, induce oxidative stress, and promote nuclear factor kappa B activation. In metabolic syndrome, which is characterized by a chronic low grade of inflammation, PGC-1α dysregulation modifies the metabolic properties of tissues by altering mitochondrial function and promoting reactive oxygen species accumulation. In conclusion, PGC-1α acts as an essential node connecting metabolic regulation, redox control, and inflammatory pathways, and it is an interesting therapeutic target that may have significant benefits for a number of metabolic diseases.
Effects of infant mortality and weaning on the onset of postpartum menstruation: hazard model analysis.

Researchers conducted a hazard model analysis of data from a birth interval study in 1976-1978 in a rural subdistrict in central Java Indonesia to determine the effects of infant death and weaning on the beginning of postpartum menstruation. Essentially all women breast fed their child for a least 6 months. The median stood at 26 months. A significant decline in breast feeding began at 14 months. Mothers began introducing solid food at a median of 2.1 months (range .8-15.3 months). The median duration of breast feeding women was 15.7 months. For mothers whose child died the median was 9.6 months. Amenorrhea for those stopped breast feeding due to weaning lasted a median of 24 months. The results showed that infant death had the strongest effect on the resumption of menses. Specifically the risk increased 7.5 times for mothers who had an infant die. The effects of infant death decreased significantly over time however. Weaning also had a positive significant effect on postpartum menstruation. Like infant death weaning was not diminished by mothers age parity and nutritional status. Liquid supplementation accelerated resumption of menses more so than did solid food supplementation. Neither maternal nutrition nor intensity of breast feeding significantly affected postpartum menstruation. Oral contraceptive use accelerated it. Research has shown that reduction in breast feeding in a population increases fertility by reducing birth intervals. Therefore reducing infant mortality in populations where breast feeding is common place will eventually bring about a natural decrease in fertility.
The Diagnostic Usefulness of Ultrasound-Guided Peritoneal Biopsy for the Solitary Peritoneal Thickening of an Unknown Cause Visualized as Only Infiltrated Fat Tissue on a CT Scan

Peritoneal lesion can be caused by several diseases such as malignancies and inflammation (1, 2). Identification of the cause of peritoneal lesion is often critical for appropriate treatment and predicting prognosis. In patients with peritoneal thickening, the diagnosis is often made on the basis of multiple factors, including the patients’ symptoms, laboratory tests and ascitic fluid analysis. But in some cases it is hard to differentiate peritoneal carcinomatosis from chronic inflammation including tuberculous peritonitis (3-6). Assessment of the pattern of peritoneal lesion in abdominal computed tomography (CT) may help to differentiate inflammatory involvement from malignant involvement. Indeed, smooth uniform peritoneal thickening is the prevalent pattern in inflammation, whereas nodular pattern is common in malignancies. The Diagnostic Usefulness of Ultrasound-Guided Peritoneal Biopsy for the Solitary Peritoneal Thickening of an Unknown Cause Visualized as Only Infiltrated Fat Tissue on a CT Scan CT 스캔에서 지방침윤만 보이는 원인 불명의 비후된 복막에 대한 초음파 유도하의 복막조직 생검의 진단적 유용성
Urban and Extra-Urban Cult Buildings in the Aegean World From the Excavation to the 3D Reconstruction

This work deals with sacred areas in the Minoan and Mycenaean world. The identification of cult sites is an open issue, since archaeology has not always provided with peculiar architectural forms for these places. In the Minoan period areas outside the urban settlement with ritual destination can be recognized mainly through appropriate finding of cult indicators (objects, benches, animal bones, altars) and on the basis of the same findings urban and palace shrines have been recognized. In the Mycenaean period cult sites belong almost exclusively to urban and palatial contexts with rather simple dimensions, not much different from domestic architecture and frequently confining with craftsman workshops. We have focused on some of the above typologies with the purpose to show how 3-dimensional reconstructions can be of great help in identifying the cult sites with confidence. With the contemporary digital solutions it's possible to show the image of the past, creating realistic environments aimed to virtual or static image creation. This process allows a better approach to the learning of the past and at the same time, when combined with the tools for investigation and analysis, creates the conditions for a step forward in the knowledge of a historical period. The purpose remains to find the best learning pathway using various tools, in the pure spirit of multimedia, which means the total interaction between multiple media in a single environment. The approach proposed here will not be limited to the simple use of digital graphic tools, but will include the traditional drawing techniques enhanced in the computer graphic environment to create more lively, suggestive and communicative representations. The novelty of our approach resides in the application of the 3-D modelling to sites that are mostly considered marginal compared to the most famous buildings but that are able to largely contribute to enlighten features that are still badly defined and quite promising.