Miracle-dz/UniFAR-FTUs huggingface dataset:
Tertiapin-Q blocks recombinant and native BK-type K channels in a use-dependent manner

Tertiapin, a short peptide from honey bee venom, has been reported to specifically block the inwardly rectifying K (Kir) channels, including GIRK1+GIRK4 heteromultimers and ROMK1 homomultimers. In the present study, the effects of a stable and functionally similar derivative of tertiapin, tertiapin-Q, were examined on recombinant human voltage-dependent Ca-activated large conductance (BK or MaxiK; α-subunit or hSlo1 homomultimers) and mouse inwardlyrectifying GIRK1+GIRK2 (i.e., Kir3.1 and Kir3.2) heteromultimeric K channels expressed in Xenopus oocytes, and on cultured newborn (P0) mouse dorsal root ganglion (DRG) neurons. In two-electrode voltage-clamped oocytes, tertiapin-Q (1-100 nM) inhibited BK-type K channels in a useand concentration-dependent manner. We also confirmed the inhibition of recombinant GIRK1+GIRK2 heteromultimers by tertiapin-Q, which had no effect on endogenous depolarizationand hyperpolarization-activated currents sensitive to extracellular divalent cations (Ca, Mg, Zn and Ba) in defolliculated oocytes. In voltage-clamped DRG neurons, tertiapinQ voltageand use-dependently inhibited outwardly rectifying K currents, but Cs-blocked hyperpolarization-activated inward currents including IH were insensitive to tertiapin, baclofen, barium and zinc, suggesting absence of functional GIRK channels in the newborn. Under current clamp conditions, tertiapin-Q blocked the action potential afterhyperpolarization (AHP) and increased action potential duration in DRG neurons. Taken together, these results demonstrate that the blocking actions of tertiapin are not specific to Kir channels and that the blockade of recombinant BK channels and native neuronal AHP currents is use-dependent. Inhibition of specific types of Kir and voltage-dependent Ca-activated K channels by tertiapin at nanomolar range via different mechanisms may have implications in pain physiology and therapy. This article has not been copyedited and formatted. The final version may differ from this version. JPET Fast Forward. Published on June 9, 2005 as DOI: 10.1124/jpet.105. 085928 at A PE T Jornals on A uust 4, 2017 jpet.asjournals. org D ow nladed from
CLEP_A_265404 1059..1072

1Division of Gastroenterology, Department of Medicine, NYU School of Medicine, New York, New York, USA; 2Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; 3Department of Pediatrics, Orebro University Hospital, Orebro, Sweden; 4Division of Epidemiology and Public Health, School of Medicine, University of Nottingham, Nottingham, UK; 5Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York, USA; 6Clinical Epidemiology Division, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; 7Sachs’ Children and Youth Hospital, Stockholm South General Hospital, Stockholm, Sweden; 8Department of Clinical Science and Education Södersjukhuset, Karolinska Institutet, Stockholm, Sweden
Recent Evaluations and Applications of a Cassava Model in Thailand: A Review

Cassava is an important economic crop in Thailand. The cassava simulation model, under the Crop Simulation Model of Decision Support System for Agrotechnology Transfer package (DSSATCSM), has been evaluated and widely applied to simulate, evaluate and predict cassava production systems and related policy scenarios. This is the first review on the system modeling and simulation approach on cassava studies in Thailand, with an emphasis on its applications and future research directions. Various research teams were also trying to explore the site-specific technology, but still in the early stage of development. Availability of reliable data sets for model calibration and evaluation is one of the main issue that users are facing with. Therefore, future needs on the development of reliable input data sets for model testing under diverse cassava production ecosystems in Thailand are required.
TemperatephagesTP901-1anduLC3,belonging to theP335species, apparentlyuse di¡erent pathways forDNA injection inLactococcus lactis subsp. cremoris 3107

TemperatephagesTP901-1anduLC3,belonging to theP335species, apparentlyuse di¡erent pathways forDNA injection inLactococcus lactis subsp. cremoris 3107 Solvej Østergaard Breum, Horst Neve, Knut J. Heller & Finn K. Vogensen Department of Food Science, Faculty of Life Science, Copenhagen University, Frederiksberg, Denmark; Centre for Advanced Food Studies (LMC), Frederiksberg, Denmark; and Institute of Microbiology, Federal Research Center for Nutrition and Food, Kiel, Germany
RETHINKING CITIZEN PARTICIPATION AND LOCAL GOVERNANCE IN POST CORONA VIRUS PANDEMIC ERA IN ZIMBABWE

The purpose of this paper is to establish the extent to which the Corona Virus Disease COVID 19 has impacted citizen participation practices in Zimbabwe. Measures put in place to reduce spread of the pandemic like social distancing and quarantines have a direct impact on previously used spaces for citizen engagements. In view of the above, this qualitative research approach used publicly available literature from articles and reports to establish existing methods and practices of citizen engagement in Zimbabwe. Taking into account the transformations brought by COVID 19 pandemic, this paper recommends that the traditional approaches to citizen participation will not be compatible with the new landscape, therefore, they need to be remodeled and complimented with new methods like adoption of ICT and community radios thereby giving communities viable options to participate in local decision making.
News on New Devices: Examining Multiplatform News Consumption in the Digital Age

News on New Devices: Examining Multiplatform News Consumption in the Digital Age Hsiang Iris Chyi, Ph.D. Assistant Professor School of Journalism The University of Texas at Austin Austin, TX 78712 Phone: (512) 471- 0553 Fax: (512) 471- 7979 E-mail: chyi@mail.utexas. edu Monica Chadha Doctoral student School of Journalism University of Texas at Austin 1 University Station, A1000 Austin, TX 78712 Phone: (914) 589-3530 E-mail: mchadha@mail.utexas. edu Paper presented at the 2011 International Symposium on Online Journalism, Austin, Texas, April 1-2, 2011
A Baseline Assessment of Soil Organic Carbon in the Mangroves of the Bakassi Peninsula South-West Cameroon

The mangrove soils as one of the global soil types is a major carbon store that helps to curb the rising global temperatures. This is not unconnected to their high carbon storing and sequestration potentials of the peat soils. The conclusion is characterized by some knowledge gaps on the actual carbon stock and sequestration potentials of some mangroves soils on the Central African Sub-regional landscape. Some of these areas are the Bakassi mangroves in the South West Cameroon. Cross-border conflicts, piracy and over exploitation have rendered the sourcing of appropriate data on its carbon stock and sequestration potentials difficult. In strive to bridge this knowledge gap, this work carried out baseline assessments of the carbon stock and sequestration rate of this peat soil. To achieve the study objectives, stratified random opportunistic sampling using an inventory design based on five forest canopy height classes, with collection of peat soils using a soil auger to different depth for laboratory analysis was done. Soils Organic Carbon stocks were estimated from soils to a depth of 100cm and determined using chromic acid digestion and spectrophotometric analysis. Parameters determined were bulk density and percentage carbon. Results showed that; soil carbon stock density ranged from 705.8 (Mg/ha) to 546.2 (Mg/ha). Thus on average, for a hectare in Bakassi, the Soil Organic carbon stock was 632.65 (Mg/ha)
Saturation based forecasting of travel time reliability in urban road transport

The objective of this paper is to provide a brief review on travel time reliability approaches, to explore the relation between reliability and the volume-capacity ratio (saturation level) based on a case study of Budapest, and considering the results to propose an adequate method, plus draft further research. In this paper reliability is analysed based on automatic measurements of journey times and traffic volumes from a dataset for the city of Budapest. Due to the limitations of measurements and data route-level analyses has been carried out. The main finding and the novelty of the study is a model which can forecast the standard deviation of travel times based on the volume-capacity ratio and the free-flow travel time. The results prove that besides existing mean-delay-based models, travel time reliability can be forecasted based on the volume-capacity ratio with an adequate accuracy.
The eigenvalues and eigenvectors of finite, low rank perturbations of large random matrices

We consider the eigenvalues and eigenvectors of finite, low rank perturbations of random matrices. Specifically, we prove almost sure convergence of the extreme eigenvalues and appropriate projections of the corresponding eigenvectors of the perturbed matrix for additive and multiplicative perturbation models. The limiting non-random value is shown to depend explicitly on the limiting eigenvalue distribution of the unperturbed random matrix and the assumed perturbation model via integral transforms that correspond to very well known objects in free probability theory that linearize non-commutative free additive and multiplicative convolution. Furthermore, we uncover a phase transition phenomenon whereby the large matrix limit of the extreme eigenvalues of the perturbed matrix differs from that of the original matrix if and only if the eigenvalues of the perturbing matrix are above a certain critical threshold. Square root decay of the eigenvalue density at the edge is sufficient to ensure that this threshold is finite. This critical threshold is intimately related to the same aforementioned integral transforms and our proof techniques bring this connection and the origin of the phase transition into focus. Consequently, our results extend the class of ‘spiked’ random matrix models about which such predictions (called the BBP phase transition) can be made well beyond the Wigner, Wishart and Jacobi random ensembles found in the literature. We examine the impact of this eigenvalue phase transition on the associated eigenvectors and observe an analogous phase transition in the eigenvectors. Various extensions of our results to the problem of non-extreme eigenvalues are discussed.
Is PCSK9 Associated with Plasma Lipid Levels in a Sub-Saharan African Population of Patients with Obesity and Type 2 Diabetes? Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Purpose: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of circulating LDL cholesterol. There are inconsistent data in some populations concerning the association between PCSK9, LDL and CRP. The emerging importance of the inhibition of PCSK9 for the treatment of hypercholesterolemia warrants investigations in different populations. The aim of this study from a Sub-Saharan African population was to evaluate the association between PCSK9 and hs-CRP levels and plasma lipid levels in patients with type 2 diabetes (T2D) and obese and lean controls. Patients and methods: A cross-sectional analytical study was conducted in a major hospital in Yaoundé, Cameroon in a cohort of 162 participants (53% females). There were 54 non-obese T2D patients matched for age and sex to 54 obese nondiabetic and 54 nondiabetic lean subjects. PCSK9 level was assessed by sandwich ELISA method and hsCRP by nephelometry. Results: PCSK9 and hs-CRP levels were signi ﬁ cantly higher in obese and T2D subjects when compared to lean controls (p<0.001 and p=0.002, respectively). The association between PCSK9 and triglyceride levels in the overall population was gender dependent (p=0.04) and subgroup analysis showed a signi ﬁ cant positive correlation between PCSK9 and triglyceride levels in males but not in females ( r =0.56, p=0.02 and r =0.2 and p=0.1, respectively). Multilinear regression analysis identi ﬁ ed BMI as an independent predictor for PCSK9 levels and this association was maintained after adjustment for confounders; adjusted β -coef ﬁ cient; 36.1 (95% CI; 29.2 – 47.4). We did not ﬁ nd an association between PCSK9 and any plasma lipid levels in obese and T2D subjects, nor between PCSK9 and hs-CRP levels. Conclusion: Obese and type 2 diabetes subjects have higher PCSK9 levels when compared to lean controls, suggesting that these metabolic states potentially impact PCSK9 levels in Cameroonian patients.
Trudel, Chantal and Cobb, Sue and Momtahan, Kathryn and Brintnell, Janet and Mitchell, Ann (2017) Human factors considerations in designing for infection prevention and control in neonatal care – findings from a pre-design

Qualitative data collection methods drawn from the early stages of human -centred design frameworks combined with thematic analysis were used to develop an understanding of infection prevention practice within an existing neonatal intensive care unit. Findings were used to generate a framework of understanding which in turn helped info rm a baseline approach for future research and design development. The study revealed that a lack of clarity between infection transmission zones and a lack of design attributes needed to uphold infection prevention measures may be undermining healthcare workers’ understanding and application of good practice. The issue may be further complicated by well intentioned behavioural attitudes to meeting work objectives; undue influences from spatial constraints; the influence of inadvertent and excessive touch-based interactions; physical and/or cognitive exertion to maintain transmission barriers; and the impact of expanding job design and increased workload to supplement for lack of effective barriers. Practitioner Summary: Despite high hand hygiene compliance within a neonatal intensive care unit, healthcare workers expressed concerns about the unit design and infection prevention practice. Early inquiry methods from human centred design and thematic analysis helped develop a framework to understand how design can be used to aid infection prevention.
Detection of cerebral ischemia in rabbits by magnetic induction phase shift spectroscopy comparison with laser Doppler flowmetry

Acute cerebral ischemia is one of the important clinical diseases that are usually detected and studied with magnetic resonance imaging, computed tomography, and positron emission tomography. Magnetic inductive phase shift (MIPS) is a new method used to detect cerebral ischemia which is non-contact, miniaturized, and low-cost. To obtain more useful information in broadband measurement of cerebral ischemia, we extended magnetic induction phase shift spectroscopy (MIPSS). A total of 25 rabbits were studied using a two-coil sensor within 0.3MHz to 200MHz frequency range in linear scanning mode, and all the subjects were measured for 1 hour. On the basis of a rabbit acute cerebral ischemia model by carotid artery occlusion, the rabbits were divided into unilateral ligation, bilateral ligation and non-ligation group. The characteristic frequency with the highest detection sensitivity was selected for further analysis and processing. Results showed that the average phase shift of transmission parameter S21 of non-ligation group, unilateral ligation group and bilateral ligation group was −0.195°±0.079°, −4.873°±1.042°, and −9.165°±2.862° respectively. MIPSS can distinguish different severities of cerebral ischemia in rabbits with a statistical significance of p<0.05. And the correlations of laser Doppler flowmetry and phase shift suggested that phase shift can reflect the blood flow changes in brain. Overall, the results suggest that the MIPSS detection method provides a potential for early detection and distinguishing different severities of acute global cerebral ischemia.
Momentum life cycle around the world

The momentum life cycle (MLC) hypothesis first proposed by Lee and Swaminathan (2000) applies also to global markets. Early-stage strategies significantly outperform the late-stage and conventional strategies in most countries. Individualism culture is positively associated with latestage but unrelated to early-stage momentum profitability, suggesting that earlyand late-stage momentums are driven by different underlying mechanisms. Consistent with Stein’s (2009) model that arbitrageurs could amplify mispricing, we find that late-stage momentum profits are more pronounced in countries with lower limits to arbitrage. Furthermore, we find that the MLC also applies to exchange traded funds in the United States. ☆ We thank Chishen Wei and seminar participants at the Hong Kong University of Science and Technology, University of Queensland, and the 2015 Asian Financial Association (Asian FA) annual meeting for their helpful comments and suggestions and Alice Cheung for her editorial assistance. All remaining errors are our own. * Corresponding author: K.C. John Wei, School of Accounting and Finance, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Tel: (852)-2766-4953; Fax: (852)-2330-9845. Email: johnwei@ust.hk. Momentum Life Cycle around the World and Beyond Abstract The momentum life cycle (MLC) hypothesis first proposed by Lee and Swaminathan (2000) applies also to global markets. Early-stage strategies significantly outperform the late-stage and conventional strategies in most countries. Individualism culture is positively associated with latestage but unrelated to early-stage momentum profitability, suggesting that earlyand late-stage momentums are driven by different underlying mechanisms. Consistent with Stein’s (2009) model that arbitrageurs could amplify mispricing, we find that late-stage momentum profits are more pronounced in countries with lower limits to arbitrage. Furthermore, we find that the MLC also applies to exchange traded funds in the United States.The momentum life cycle (MLC) hypothesis first proposed by Lee and Swaminathan (2000) applies also to global markets. Early-stage strategies significantly outperform the late-stage and conventional strategies in most countries. Individualism culture is positively associated with latestage but unrelated to early-stage momentum profitability, suggesting that earlyand late-stage momentums are driven by different underlying mechanisms. Consistent with Stein’s (2009) model that arbitrageurs could amplify mispricing, we find that late-stage momentum profits are more pronounced in countries with lower limits to arbitrage. Furthermore, we find that the MLC also applies to exchange traded funds in the United States.
COPD_A_291967 499..517

1Respiratory Epidemiology and Clinical Research Unit, Department of Medicine, McGill University and Research Institute of the McGill University Health Centre, Montreal, QC, Canada; 2Nuffield Department of Medicine, University of Oxford, Oxford, Oxfordshire, UK; 3Respiratory Therapy Area, GlaxoSmithKline, Brentford, Middlesex, UK; 4William Harvey Institute, Bart’s and the London School of Medicine and Dentistry, London, UK; 5Clinical Sciences, GlaxoSmithKline, Collegeville, PA, USA; 6Pulmonary, Allergy and Critical Care Division, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; 7Institute of Infection and Immunity, St George’s, University of London, London, UK; 8University of Leicester, Leicester, UK; 9University of Exeter Medical School, College of Medicine and Health, University of Exeter, Exeter, UK Abstract: Chronic obstructive pulmonary disease (COPD) is associated with major healthcare and socioeconomic burdens. International consortia recommend a personalized approach to treatment and management that aims to reduce both symptom burden and the risk of exacerbations. Recent clinical trials have investigated single-inhaler triple therapy (SITT) with a long-acting muscarinic antagonist (LAMA), long-acting β2-agonist (LABA), and inhaled corticosteroid (ICS) for patients with symptomatic COPD. Here, we review evidence from randomized controlled trials showing the benefits of SITT and weigh these against the reported risk of pneumonia with ICS use. We highlight the challenges associated with crosstrial comparisons of benefit/risk, discuss blood eosinophils as a marker of ICS responsiveness, and summarize current treatment recommendations and the position of SITT in the management of COPD, including potential advantages in terms of improving patient adherence. Evidence from trials of SITT versus dual therapies in symptomatic patients with moderate to very severe airflow limitation and increased risk of exacerbations shows benefits in lung function and patient-reported outcomes. Moreover, the key benefits reported with SITT are significant reductions in exacerbations and hospitalizations, with data also suggesting reduced all-cause mortality. These benefits outweigh the ICS-class effect of higher incidence of study-reported pneumonia compared with LAMA/LABA. Important differences in trial design, baseline population characteristics, such as exacerbation history, and assessment of outcomes, have significant implications for interpreting data from cross-trial comparisons. Current understanding interprets the blood eosinophil count as a continuum that can help predict response to ICS and has utility alongside other clinical factors to aid treatment decision-making. We conclude that treatment decisions in COPD should be guided by an approach that considers benefit versus risk, with early optimization of treatment essential for maximizing long-term benefits and patient outcomes.
Culture moderates the link between perceived obligation and biological health risk: Evidence for culturally distinct pathways to achieving positive health outcomes

Rationale: Although perceived obligations to meet the expectations of family, friends, and society can be detrimental to physical health, much research in this area has thus far been conducted exclusively on Western samples. Cross-cultural research importantly suggests that positive health can be dependent on whether one engages in modes of being that are sanctioned by one's culture. Specifically, studies show that better health is predicted when people from cultures that value independence are able to exercise their personal autonomy and when people from cultures that value interdependence are able to maintain relational harmony (Kitayama et al.,
DESCRIPTIVE ANALYSIS OF THE SIBILNG RELATIONSHIPS FOR FAMILES WITH A CHILD WITH AUTISM SPECTRUM DISORDERS

The present study examined mothers’ and sisters’ perceptions of the sibling relationship for children who have a sibling with autism. The study occurred within the context of a strengths-based computer workshop called the iStar SketchUp Workshops. The siblings were involved in the workshops along with their brothers. Seven siblings and 6 parents were interviewed to see how they perceived the sibling relationship within the context of everyday life and within the context of the workshops. Using thematic coding, themes from the interviews were identified. The themes highlighted differences in the way that the siblings and parents framed the sibling relationship within everyday life activities versus within the workshops. Within the context of everyday life activities, the siblings focused on helping their brothers and playing with one another. The siblings also described how they had a sense of duty to their brothers because they were family. On the contrary, the mothers focused on the challenges of the sibling relationship, which they attributed to differences in learning style, which ultimately led to their perceptions of siblings engaging in separate activities. In contrast, both the siblings and parents perceived their brothers and sons differently in the context of the workshops. In the context of the workshops, they saw them as boys who possessed amazing skills and fit in with their peers. The sisters had a sense of pride for their brothers’ accomplishments and the mothers spoke more positively about their children’s relationships. Implications for interventions for children with autism spectrum disorders are discussed.