Miracle-dz/UniFAR-FTUs huggingface dataset:
Stimulation and Isolation of Paraphysoderma sedebokerense (Blastocladiomycota) Propagules and Their Infection Capacity Toward Their Host Under Different Physiological and Environmental Conditions

Paraphysoderma sedebokerense (P. sedebokerense) (Blastocladiomycota) is a facultative pathogenic chytrid that causes irreversible damage to some green microalgae. Specific attacks leading to culture collapse under different conditions have only been described in the lucrative microalga Haematococcus pluvialis (H. pluvialis), while generating biomass for ketocarotenoid astaxanthin production, both indoors and outdoors. In order to manage the infection, parasite propagules (zoospores/amoeboid swarmers), the initiators of the disease, must be studied. Until now, no report on isolated P. sedebokerense propagules has been published. Here, we report on a reproducible method for the stimulation of P. sedebokerense propagule release and their isolation from fungal cultures in synthetic media and infected H. pluvialis cultures, and we further studied their development under different conditions. The isolated propagules featured different spore morphotypes, with coatless spherical spores and amoeboid swarmers being the most dominant in the first pulse of propagule release in both cultures. Inoculating the pure propagules with the host, in both the presence and absence of nitrogen, resulted in epidemic development in both green and red cells; however, in red cells, the epidemic developed more quickly in the presence of nitrogen. Biologically non-active autoclaved host cells were used to distinguish the initial stages of recognition from more progressive stages of the epidemics; on these cells, propagules encysted but did not develop further. These results prove the existence of heat-stable recognition sites on the host and an obligatory signal transduction from the host to support fungal cyst development. The propagule isolation method described herein is a breakthrough that will enable researchers to study the influence of different substances on the propagules, specifically as the initiators of the infection, and thus assist in the management of chytrid diseases. Moreover, it will be useful in studying host-parasite recognition and, therefore, will increase our understanding of the multiple chytrid infections found in nature.
Relationship Between Sleep Quality and Mood: Ecological Momentary Assessment Study

Background Sleep disturbances play an important role in everyday affect and vice versa. However, the causal day-to-day interaction between sleep and mood has not been thoroughly explored, partly because of the lack of daily assessment data. Mobile phones enable us to collect ecological momentary assessment data on a daily basis in a noninvasive manner. Objective This study aimed to investigate the relationship between self-reported daily mood and sleep quality. Methods A total of 208 adult participants were recruited to report mood and sleep patterns daily via their mobile phones for 6 consecutive weeks. Participants were recruited in 4 roughly equal groups: depressed and anxious, depressed only, anxious only, and controls. The effect of daily mood on sleep quality and vice versa were assessed using mixed effects models and propensity score matching. Results All methods showed a significant effect of sleep quality on mood and vice versa. However, within individuals, the effect of sleep quality on next-day mood was much larger than the effect of previous-day mood on sleep quality. We did not find these effects to be confounded by the participants’ past mood and sleep quality or other variables such as stress, physical activity, and weather conditions. Conclusions We found that daily sleep quality and mood are related, with the effect of sleep quality on mood being significantly larger than the reverse. Correcting for participant fixed effects dramatically affected results. Causal analysis suggests that environmental factors included in the study and sleep and mood history do not mediate the relationship.
Distinct Sources of Variability Affect Eye Movement Preparation

The sequence of events leading to an eye movement to a target begins the moment visual information has reached the brain, well in advance of the eye movement itself. The process by which visual information is encoded and used to generate a motor plan has been the focus of substantial interest partly because of the rapid and reproducible nature of saccadic eye movements, and the key role that they play in primate behavior. Signals related to eye movements are present in much of the primate brain, yet most neurophysiological studies of the transition from vision to eye movements have measured the activity of one neuron at a time. Less is known about how the coordinated action of populations of neurons contribute to the initiation of eye movements. One cortical area of particular interest in this process is the frontal eye fields, a region of prefrontal cortex that has descending projections to oculomotor control centers. We recorded from populations of frontal eye field neurons in macaque monkeys engaged in a memory-guided saccade task. We found a variety of neurons with visually evoked responses, saccade-aligned responses, and mixtures of both. We took advantage of the simultaneous nature of the recordings to measure variability in individual neurons and pairs of neurons from trial-to-trial, as well as the moment-to-moment population activity structure. We found that these measures were related to saccadic reaction times, suggesting that the population-level organization of frontal eye field activity is important for the transition from perception to movement. SIGNIFICANCE STATEMENT The transition from perception to action involves coordination among neurons across the brain. In the case of eye movements, visual and motor signals coexist in individual neurons as well as in neighboring neurons. We used a task designed to compartmentalize the visual and motor aspects of this transition and studied populations of neurons in the frontal eye fields, a key cortical area containing neurons that are implicated in the transition from vision to eye movements. We found that the time required for subjects to produce an eye movement could be predicted from the statistics of the neuronal response of populations of frontal eye field neurons, suggesting that these neurons coordinate their activity to optimize the transition from perception to action.
Surgical process modelling strategies: which method to choose for determining workflow?

Abstract The vital role of surgeries in healthcare requires a constant attention to improvement. Surgical process modelling is an innovative and rather recently introduced approach for tackling the issues in today’s complex surgeries. This modelling field is very challenging and still under development, therefore, it is not always clear which modelling strategy would best fit the needs in which situations. The aim of this study was to provide a guide for matching the choice of modelling strategies for determining surgical workflows. In this work, the concepts associated with surgical process modelling are described, aiming to clarify them and to promote their use in future studies. The relationship of these concepts and the possible combinations of the suitable approaches for modelling strategies are elaborated and the criteria for opting for the proper modelling strategy are discussed.
Lesbian and Gay Individual Parenting Desires in Heteronormative Contexts

The cultural, social and institutional barriers that LGBT (Lesbian, Gay, Bisexual, Transgender) individuals have to face play crucial roles in their desires and intentions to have children. However, unlike the many studies on the decision-making process in the transition to parenthood, few studies have analysed the origins of parenting desires and intentions among LGBT individuals. This study explores the desires and intentions to have children amongst a sample of childless lesbian and gay Italian individuals. A sample of 285 participants (127 women and 158 men) completed a research protocol composed of items evaluating the strength of their desire to have children, their intentions about having children and their general attitudes towards parenting. The findings revealed how, despite the persisting depth of heteronormativity in the country and the absence of legal protection for lesbian and gay parents, a large percentage of participants expressed the desire and intention to have a child. These parenting intentions would seem to be positively influenced mainly by the negative attitudes towards childlessness and by the value attributed to parenthood.
Managing Channel Profits When Retailers Have Profitable Outside Options

The channel-coordination literature typically focuses on how a supplier canovercome channel inefficiencies stemming from misaligned pricing incentives. In contrast, we show that when an incumbent supplier faces competition from other suppliers to supply the downstream firms, it may want to create inefficiencies. Our analysis offers useful prescriptions for how incumbent suppliers should react to competitive threats by smaller competitors, how manufacturers should react to powerful retailers who can produce their own private-label brands, and how upstream firms should optimally treat downstream firms who may have different marginal costs of distribution. Our analysis also explains why wholesale prices and thus final-goods prices would be expected to decrease when there is an increase in upstream or downstream competition.
Clostridium difficile Infection Epidemiology over a Period of 8 Years—A Single Centre Study

Clostridium difficile infection (CDI) is the most common infectious disease related to antibiotic-associated diarrhoea and is a current leading cause of morbidity/mortality, with substantial consequences for healthcare services and overall public health. Thus, we performed a retrospective epidemiological study of CDI for a long period (8 years), in an infectious hospital located in north-western Romania, which serves an entire county of the country (617,827 inhabitants). From 2011 to 2018, 877 patients were diagnosed with CDI; the mean incidence of this disease was 2.76 cases/10,000 patient-days, with an increasing trend in the annual incidence until 2016, at which point there was a decrease. The most commonly afflicted were patients in the 75–84 age group, observed in winter and spring. The results show that the antibiotics were administered in 679 (77.42%) subjects, within the last 3 months before CDI, statistically significant more than proton-pump inhibitors (PPIs)—128 (14.60%) and antidepressant medications—60 (6.84%), which were administered during the same period (p < 0.001). No medication was reported in 10 (6.84%) cases of CDI, in the last 3 months of the study. The fatality rate attained 4.1%, tripling in 2018 vs. 2011. CDI became a significant public health conundrum that can, nevertheless, be combatted through a judicious use of antibiotics.
Linkages between Economic Growth, Poverty and Environmental Quality in Indonesia

The biggest obstacle to sustainable development in Indonesia is due to social-environmental factors. The objective of this study is to identify lever variables in the intended socio-environmental factors through dimensional analysis in sustainable development. By using the path analysis methods and secondary data on economic growth, the number of poor people and an index of environmental quality in Indonesia, 2016, it can be proven that poverty has a direct negative effect on environmental quality. This makes it possible to occur in the rural poverty typology because their needs for life depend on natural resources. Therefore, poverty reduction policies should be prioritized in reducing the number of poor people in rural areas.
Biogenic synthesis and characterization of silver nanoparticles using aqueous leaf extract of Scoparia dulcis L. and assessment of their antimicrobial property

Abstract Naturally occurring phytochemicals serve as an excellent substitute in synthesizing nanomaterials. A process for the synthesis of silver nanoparticles (AgNPs) from the aqueous leaf extract of naturally occurring Scoparia dulcis is described here. The extracellular formation of AgNPs occurred within few minutes upon incubation of S. dulcis aqueous leaf extract (0.1 mL) (100% extract) with silver nitrate (2 mM AgNO3) at 90 °C for 30 min, is first of its kind work. The appearance of bright yellow color with λmax 420 nm confirm the formation of AgNPs. Zeta potential and X-ray diffraction (XRD) studies reveal stable AgNPs (−22.7 mV) and characteristic spectra for silver. Fourier transform infrared (FTIR) spectroscopy indicate the involvement of carboxyl, amine and hydroxyl groups in the synthetic process. Transmission electron microscopy (TEM) show the spherical nature of AgNPs measuring 3–18 nm in size. Additional characterization using Dynamic light scattering (DLS) revealed the average particle size distribution of AgNPs as around 8.2 nm. Further antimicrobial testing through agar disc diffusion plate method indicated that silver nanoparticles are potentially active against pathogenic bacteria such as Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus and are only optimally active against fungi such as Aspergillus niger and Candida albicans and measurement of minimal inhibition concentration by standard microdilution method. In conclusion, the study suggests that successful synthesis of green nanoparticles (AgNPs) using aqueous S. dulcis leaf extract is simple, rapid, environmentally benign and economical. Moreover, these synthesized silver nanoparticles showed antimicrobial activity.
Motivating User-Generated Content with Performance Feedback: Evidence from Randomized Field Experiments

We design a series of online performance feedback interventions that aim to motivate the production of user-generated content (UGC). Drawing on social value orientation (SVO) theory, we develop a novel set of alternative feedback message framings, aligned with cooperation (e.g., your content benefited others), individualism (e.g., your content was of high quality), and competition (e.g., your content was better than others). We hypothesize how gender (a proxy for SVO) moderates response to each framing, and we report on two randomized experiments, one in partnership with a mobile-app–based recipe crowdsourcing platform, and a follow-up experiment on Amazon Mechanical Turk involving an ideation task. We find evidence that cooperatively framed feedback is most effective for motivating female subjects, whereas competitively framed feedback is most effective at motivating male subjects. Our work contributes to the literatures on performance feedback and UGC production by introducing cooperative performance fe.
The (Neural) Dynamics of Stochastic Choice

The standard framework for modeling stochastic choice, the random utility model, is agnostic about the temporal dynamics of the decision process. In contrast, a general class of bounded accumulation models from psychology and neuroscience explicitly relate decision times to stochastic choice behavior. This article demonstrates that a random utility model can be derived from the general class of bounded accumulation models, and characterizes how the resulting distribution of random utility depends on response time. This relationship can bias the estimation of structural preference parameters. The bias can be alleviated via the inclusion of standard observables directly in the econometric specification, or through incorporating novel observables such as response time or neurobiological data. Examples of estimating risk and brand preferences are pursued. This paper was accepted by Matthew Shum, marketing.
Developmental, genetic and environmental variations of global DNA methylation in the first leaves emerging from the shoot apical meristem in poplar trees

ABSTRACT In the context of climate changes, clarifying the causes underlying tree phenotypic plasticity and adaptation is crucial. Studies suggest a role of epigenetic mechanisms in response to external stimuli, raising the question whether such processes can promote acclimation of trees exposed to adverse climate conditions. Recently, we revealed an environmental epigenetic footprint in the shoot apical meristem (SAM) which could partially be transmitted mitotically, for several months, up until the winter-dormant bud in field conditions. Here, we extended our previous analysis to the leaves of the same P. deltoides×P. nigra clones. We aimed at estimating the range of developmentally, genetically, and environmentally induced variations on DNA methylation. We showed that only the first leaves emerging from the SAM displayed variations of DNA methylation under changing water conditions. We also found that these variations are genotype- and pedoclimatic site-dependent. Altogether, our data raised questions and perspectives on the direct acquisition, the maintenance of environmentally induced DNA methylation changes, and their mitotic transmission from the SAM to the first emerging leaves.
School Age Neurological and Cognitive Outcomes of Fetal Growth Retardation or Small for Gestational Age Birth Weight

Children who were growth restricted in utero (FGR) and are born small for gestational age (SGA) may experience poorer long term neurological and cognitive outcomes. Those also born preterm may have particular difficulties. The objective of this paper was to review the literature on school age neurocognitive outcome for term and preterm children that was published in the last 15 years. Considering term born children first, there is evidence that these children are at higher risk for Cerebral Palsy (CP) than those born appropriate for gestational age (AGA); information on neuromotor function in the absence of CP is somewhat contradictory. With regards to cognitive outcome, the most common finding was that being born SGA and/or FGR at term does not impact negatively on general intellectual functioning, commonly assessed by IQ scores. There was some indication that they may experience particular problems with attention. With regards to children born preterm, the risk of CP appears not to be increased compared to those preterms born AGA. For preterm children who do not develop CP, motor outcome is more affected by post-natal and post-neonatal brain growth than intrauterine growth. In contrast to term born children, preterm SGA and/or FGR children are at increased risk of cognitive and behavioral difficulties, and in common with term born children, are at higher risk than their AGA counterparts of difficulties with attentional control. In conclusion, preterm born SGA and/or FGR children are at higher risk of neurodevelopmental problems in the school years. It is important to continue to follow up children into the school age years because these difficulties may take time to emerge, and may be more visible in the more demanding school environment.
Land Use/Land Cover Changes and Associated Impacts on Water Yield Availability and Variations in the Mereb‐Gash River Basin in the Horn of Africa

Climate variability and drought are increasing in the Horn of Africa. Evaluating land use/land cover (LULC) changes and their impacts on water availability and variation are vital for regional land use planning and water resources management. LULC changes during 2000–2015 were estimated using high‐resolution Landsat images and the Google Earth Engine cloud platform, and land use dynamic index (K). The impact of LULC change on water yield was evaluated using the Integrated Valuation of Ecosystem Services and Tradeoff (InVEST) model. The results at a regional scale show that there were rapid decreases in the area of forests and barren lands (‐K) while there was a drastic increase in the built‐up area (+K values). The transition was found to occur from forested land to low and very low biomass areas with 51.13% and 16.7%, respectively. There were similar LULC changes in the Mereb‐Gash River Basin. The mean annual water yield increased for all the catchments during 2000–2015 and with the peak in 2005. The highest annual sum water yield decreased in the forested lands from 43.18 million m3 in 2000 to 4.1 million m3 in 2015. There was a strong positive correlation between areal changes (%) and the annual water yield variations (%) for all the LULC types except for water body, and the correlation was significantly positive for forest (p < 0.01). The study demonstrates that the decrease in forested areas and expansion in the built‐up areas had large impacts on water yield. The impacts may increase pressure on ecosystem services, exacerbate water scarcity, and food insecurity.
Identification of two clip domain serine proteases involved in the pea aphid's defense against bacterial and fungal infection

Phenoloxidases (POs) are required for the pea aphid's defense against bacterial and fungal infection. Prophenoloxidases (PPOs) are proteolytically converted to its active form PO through a clip domain serine protease cascade. In this study, we identified five clip domain serine proteases in the pea aphids. The messenger RNA levels of two of them, Ap_SPLP and Ap_VP, were upregulated by Gram‐positive bacterium Staphylococcus aureus and fungus Beauveria bassiana infections. Double‐stranded RNA‐based expression knockdown of these two genes resulted in reduced PO activity of the aphid hemolymph, higher loads of S. aureus and B. bassiana in the aphids, and lower survival rates of the aphids after infections. Our data suggest that Ap_SPLP and Ap_VP are involved in PPO activation pathway in the pea aphid.
Response of broiler chickens to xylanase and butyrate supplementation.

A 2 × 2 factorial experiment was used to evaluate the effect of xylanase and sodium butyrate supplementation on performance, intestinal fermentation, histology, and morphometry in broiler chickens. A total of 384 Ross 308 broiler chicks (1-day-old) were allocated to 4 experimental treatments: CTR (control diet), XYL (CTR diet with 16,000 BXU/kg of xylanase), BUT (CTR diet with 1 kg/t sodium butyrate), and XYL+BUT (CTR diet plus xylanase and sodium butyrate). Each treatment had 8 replicates of 12 animals. Starter and grower diets, based on wheat and soybean meal, and water were available ad libitum. Body weight gain and feed intake were measured from 0 to 42 D, and feed conversion ratio corrected for mortality (FCR) was calculated. The profile of short-chain fatty acids in the duodenum, jejunum, ileum, and ceca digesta on days 21 and 42 was analyzed in addition to the relative weights of the different portions of the gastrointestinal tract (GIT). The villus height (VH), crypt depth (CD), and villus to crypt (VH: CD) ratio from the ileal tissue on day 42 were also evaluated. Statistical comparisons were performed using a 2-way ANOVA. Xylanase supplementation improved 42-D FCR by 5 points (P = 0.006), while butyrate did not affect 42-D FCR. On day 21, birds fed butyrate had heavier total GIT (P = 0.024), duodenum (P < 0.001), and jejunum (P = 0.025). Xylanase did not influence the relative weights in any intestinal section except the crop which was smaller in xylanase supplemented birds. At day 42, the VH: CD ratio was increased with sodium butyrate (P = 0.005). Supplementation of broiler diets with xylanase improved performance but had little effect on intestinal measures, whereas sodium butyrate influenced many of the intestinal indices with no consequence on animal performance.