Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of Two Mitogenomes of Hyla sanchiangensis (Anura: Hylidae), with Phylogenetic Relationships and Selection Pressure Analyses of Hylidae

Simple Summary Hylidae is a rich family of Anura that is widely distributed across the world. Previous studies have shown that the Hyla and Dryophytos genera in the Hylidae used to belong to the same genus. The Eurasian species (including Hyla sanchiangensis) originated in North America and spread through the Beringian Land Bridge to Asia during the last ice age. Adaptation to low temperatures may require more energy expenditure. Mitochondria are the center of energy metabolism and, through oxidative phosphorylation, provide most of the ATP energy for the physiological and biochemical activities of the body. The mitogenome was used to investigate whether Hyla and Dryophytos genera are subject to positive selection. In addition, as a unique species in China, the distinction between Hyla sanchiangensis (Anura: Hylidae) and two different sites was also compared and analyzed in this paper. Abstract Hyla sanchiangensis (Anura: Hylidae) is endemic to China and is distributed across Anhui, Zhejiang, Fujian, Guangdong, Guangxi, Hunan, and Guizhou provinces. The mitogenomes of H. sanchiangensis from two different sites (Jinxiu, Guangxi, and Wencheng, Zhejiang) were sequenced. Phylogenetic analyses were conducted, including 38 mitogenomes of Hylidae from the NCBI database, and assessed the phylogenetic relationship of H. sanchiangensis within the analyzed dataset. Two mitogenomes of H. sanchiangensis showed the typical mitochondrial gene arrangement with 13 protein-coding genes (PCGs), two ribosomal RNA genes (12S rRNA and 16S rRNA), 22 transfer RNA (tRNA) genes, and one non-coding control region (D-loop). The lengths of the 12S rRNA and 16S rRNA genes from both samples (Jinxiu and Wencheng) were 933 bp and 1604 bp, respectively. The genetic distance (p-distance transformed into percent) on the basis of the mitogenomes (excluding the control region) of the two samples was calculated as 4.4%. Hyla sanchiangensis showed a close phylogenetic relationship with the clade of (H. annectans + H. tsinlingensis), which was supported by ML and BI analyses. In the branch-site model, five positive selection sites were found in the clade of Hyla and Dryophytes: Cytb protein (at position 316), ND3 protein (at position 85), and ND5 protein (at position 400) have one site, respectively, and two sites in ND4 protein (at positions 47 and 200). Based on the results, we hypothesized that the positive selection of Hyla and Dryophytes was due to their experience of cold stress in historical events, but more evidence is needed to support this conclusion.
Molecular characterization and polymorphism studies of exon 10 of the Follicle stimulating hormone receptor (FSHR) gene in Indian cattle breeds

FSH receptors are important binding sites of the follicle stimulating hormone (FSH) in ovaries and are coded by the FSHR gene which has 10 exons and 9 introns. Exon 10 is the largest (>1200 bp) of all the exons. In the present study, exon 10 of the FSHR gene partial coding sequence (CDS) was cloned and characterized in Sahiwal and Hariana cattle breeds, and DNA polymorphism was investigated using AluI/PCR-RFLP assay. The partial CDS of the Sahiwal and Hariana FSHR exon 10 was 99.3% to 100% similar to exotic cattle breeds at the nucleotide and amino acid level. A missense mutation was found in Sahiwal and Hariana at position 1118 (C→G) that caused an amino acid change at 373 (Thr→Ser) and two nonsense mutations were found at position 729 (G→A), 1180 (C→T). Phylogenetic analysis clearly showed that Sahiwal and Hariana cattle are more closely related to yak and Bos taurus. The 306 bp region of exon 10 on digestion with AluI restriction enzyme revealed three types of genotypes, namely: CC (243 bp and 63 bp), GG (193 bp, 63 bp and 50 bp) and CG (243 bp, 193 bp, 63 bp and 50 bp), where the CG genotype was more frequent (45.0%) than CC (13.5%) and GG (41.5%) genotypes, and the frequency of the G allele was higher (0.64) than the C allele (0.36) in all the screened animals. Chi square (χ2) analysis revealed that the screened animal population was in Hardy-Weinberg equilibrium. An association study revealed a significant (P<0.05) difference between Total milk yield and Lactation period where the CC genotype showed a higher milk yield than other genotypes.
Observer-Based Event-Triggered Iterative Learning Consensus for Locally Lipschitz Nonlinear MASs

This article aims to realize the robust tracking for nonidentical locally Lipschitz nonlinear multiagent systems (MASs) with unmeasurable states, for which an observer-based distributed event-triggered iterative learning control (ILC) framework is proposed. With this framework, distributed state observers provide the indispensable state information for agents to learn to complete the task. An initial state learning strategy is implemented to relax consistent initial conditions, which can improve the learning accuracy. An event-triggering mechanism is designed to reduce the occupation of communication and computing resources during the iterative learning process of MASs. With locally Lipschitz nonlinearities and iteration-varying uncertainties caused by nonrepetitive initial states, the double-dynamics analysis (DDA) method is adopted to illustrate the convergence of the ILC process. By setting appropriate learning gains and constructing a quasi-globally Lipschitz condition via the DDA method, the robust convergence can be achieved for MASs in the presence of switching topologies. A numerical simulation is used to show the validity of the proposed framework.
Complications of Percutaneous and Surgical Gastrostomy Placements in Children: a Single-Centre Series

Objectives: Gastrostomy placement is a standard procedure for children requiring enteral feeding for more than 3–6 weeks. Various techniques have been described (percutaneous endoscopic, laparoscopy, and laparotomy), and many complications have been reported. In our center, gastrostomy placement is performed either percutaneously by pediatric gastroenterologists, by laparoscopy/laparotomy by the visceral surgery team, or jointly, that is laparoscopic-assisted percutaneous endoscopic gastrostomy. This study aims to report all complications and identify risk factors and ways to prevent them. Methods: This is a monocentric retrospective study including children younger than 18 years who underwent gastrostomy placement (percutaneous or surgical) between January 2012 and December 2020. Complications that occurred up to 1 year after placement were collected and classified according to their time of onset, degree of severity, and management. A univariate analysis was conducted to compare the groups and the occurrence of complications. Results: We established a cohort of 124 children. Sixty-three (50.8%) presented a concomitant neurological disease. Fifty-nine patients (47.6%) underwent endoscopic placement, 59 (47.6%) surgical placement, and 6 (4.8%) laparoscopic-assisted percutaneous endoscopic gastrostomy. Two hundred and two complications were described, including 29 (14.4%) major and 173 (85.6%) minor. Abdominal wall abscess and cellulitis were reported 13 times. Patients who underwent surgical placement presented more complications (major and minor combined) with a statistically significant difference compared with the endoscopic technique. Patients with a concomitant neurological disease had significantly more early complications in the percutaneous group. Patients with malnutrition had significantly more major complications requiring endoscopic or surgical management. Conclusion: This study highlights a significant number of major complications or complications requiring additional management under general anesthesia. Children with a concomitant neurological disease or malnutrition are at greater risk of severe and early complications. Infections remain a frequent complication, and prevention strategies should be reviewed.
Driven to succeed, or to leave? The variable impact of self-leadership in rideshare gig work

Abstract The nature of gig work – temporary, task-based work that is facilitated by applications and platforms – lacks the structural, interpersonal, and organizational dynamics of leadership. Instead, algorithms and software are the primary source of organizational and informational resources for a gig worker in a process known as ‘algorithmic management’. Job performance and career success depends primarily on the individual gig worker’s ability to establish objectives, identify strategies, and develop skills for job performance on their own. As such, success in gig work is likely to be connected to self-leadership ability. Drawing on the psychological perspectives of control theory and conservation of resources theory, we find that the agency provided by self-leadership is generally beneficial to rideshare drivers (N = 924) in that it increases both well-being and the intention to seek non-gig work. However, enacting this agency as a leaderless worker is an intensive process, and can either be a resource gain or drain. We find that workers experience better or worse outcomes, respectively, under circumstances of high job satisfaction or high financial stress. Implications and directions for future HRM research and practice among other gig and traditional worker populations are discussed.
Saddlepoint approximations for subordinator processes

ABSTRACT We develop the saddlepoint approximations in obtaining the transition functions for general subordinator processes. We derive explicit expressions of the first- and second-order approximations. Specifically, we consider some particular classes of subordinators including the Poisson processes, the Gamma processes, the α-stable subordinators, and the Poisson random integrals. We test this technique on the Poisson and Gamma processes, which have closed-form transition functions. Outcomes show that the approximate expressions are consistent with the true transition functions. We then use this method to predict transition density functions for the α-stable subordinator processes. Finally, we calculate approximated transition densities for some Poisson random integrations. Numerical analysis shows the perfect ability of the saddlepoint approximations to predict the transition densities of the α-stable processes and the Poisson random integrations.
FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression

Aim Histamine decarboxylase (HDC) catalyzes decarboxylation of histidine to generate histamine. This enzyme affects several biological processes including inflammation, allergy, asthma, and cancer, although the underlying mechanism is not fully understood. The present study provides a novel insight into the relationship between the transcription factor FLI1 and its downstream target HDC, and their effects on inflammation and leukemia progression. Methods Promoter analysis combined with chromatin immunoprecipitation (ChIp) was used to demonstrate binding of FLI1 to the promoter of HDC in leukemic cells. Western blotting and RT-qPCR were used to determine expression of HDC and allergy response genes, and lentivirus shRNA was used to knock-down target genes. Proliferation, cell cycle, apoptosis assays and molecular docking were used to determine the effect of HDC inhibitors in culture. An animal model of leukemia was employed to test the effect of HDC inhibitory compounds in vivo. Results Results presented herein demonstrate that FLI1 transcriptionally regulates HDC by direct binding to its promoter. Using genetic and pharmacological inhibition of HDC, or the addition of histamine, the enzymatic product of HDC, we show neither have a discernable effect on leukemic cell proliferation in culture. However, HDC controls several inflammatory genes including IL1B and CXCR2 that may influence leukemia progression in vivo through the tumor microenvironment. Indeed, diacerein, an IL1B inhibitor, strongly blocked Fli-1-induced leukemia in mice. In addition to allergy, FLI1 is shown to regulate genes associated with asthma such as IL1B, CPA3 and CXCR2. Toward treatment of these inflammatory conditions, epigallocatechin (EGC), a tea polyphenolic compound, is found strongly inhibit HDC independently of FLI1 and its downstream effector GATA2. Moreover, the HDC inhibitor, tetrandrine, suppressed HDC transcription by directly binding to and inhibiting the FLI1 DNA binding domain, and like other FLI1 inhibitors, tetrandrine strongly suppressed cell proliferation in culture and leukemia progression in vivo. Conclusion These results suggest a role for the transcription factor FLI1 in inflammation signaling and leukemia progression through HDC and point to the HDC pathway as potential therapeutics for FLI1-driven leukemia.
A Single Institution Experience with Solid Pseudopapillary Neoplasm of the Pancreas: Clinicopathological Correlation and Review of the Literature

Introduction: SPN is a rare, low-grade malignancy that accounts for 1-3% of all pancreatic neoplasms and tends to affect young females. It can be asymptomatic or present with abdominal pain, and surgical resection is the only curative treatment. Methods: A retrospective study was conducted on patients with biopsy proven Solid Pseudopapillary Neoplasm (SPN) of the pancreas between January 2016 and June 2021. The study included 14 cases, including 7 resection specimens and 7 biopsies, and was conducted at a tertiary referral center in PakistanResults: All 14 patients were female and ranged in age from 15 to 48 years old, with a mean age of 24. The most common symptom was abdominal pain, and the head of the pancreas was the most common location of the tumor. Tumor size ranged from 5 cm to 18 cm, and one patient had lymph node involvement and two patients had hepatic metastasis. Most patients received surgical treatment, and follow-up information was available for 8 out of the 14 patients, who were all alive with no recurrence or metastasis. Conclusion: Due to its rarity, non-specific clinical presentation, and imaging findings, it can be challenging to diagnose. There is a lack of data on SPN from South Asia, so this study provides valuable insights into the occurrence and treatment of SPN in this region.
Strategy To Increasie Corporate Value Through Mediation Of Financing Posture And Profitability

Increasing the value is important for the corporate, the determinants of which include financing posture, profitability, growth, and size. This research aims to prove the intermediary role of financing posture and profitability in the strategy of increasing corporate value. Data was collected from companies in the LQ 45 index group February to July 2022. The results prove that profitability and size have a substantial positive contribution on corporate value. The financing posture and growth have a substantial negative contribution on corporate value. Profitability and size have a substantial negative contribution on financing posture. Growth has a non-substantial positive contribution on financing posture, but a substantial negative contribution on profitability. The financing posture mediates the contribution of profitability and size on corporate value, but does not mediate the contribution of growth on corporate value. Profitability is a semi-mediation variable for the contribution of growth on corporate value.
Adults Myelodysplastic Syndromes in Algeria: A Study by the Algerian MDS Group

Introduction: Myelodysplastic syndromes (MDS) are a group of haematological disorders, whose diagnosis is based mainly on cytological studies of blood and marrow cells and cytogenetic analysis. Moreover, national epidemiological data on MDS are very scarce, especially in Maghreb countries where the population is on average younger than in Europe or the USA. The objective of the present study was to describe demographic and clinical features and the overall survival of patients with MDS in Algeria. Materials and Methods: This study is retrospective and national multicenter (n=19 centres), performed between 2014 to 2019. The evaluation was performed using EPI-INFO and SPSS version 21 software. Survival data were calculated using the Kaplan-Meier method and comparison of survival curves using the Log Rank test. Univariate and multivariate analysis of survival was performed using the Cox regression method. The study has been approved by the Ethical and Scientific Council of the participating hospitals. The closing date of the study is 31/12/2019. Results: A total of 670 patients with newly diagnosed MDS have been identified. The average number of new cases was 112/year, with an annual progression rate of 19%. Demographics show a slight female predominance (M/F of 317/353=0, 89; sex ratio F/M=1.11). The median follow-up was 29,3 months (range, 1 to 77 months). The overall median age was 69 years (range 16-96). The crude mean annual incidence rate was 0, 38 per 100,000 inhabitants aged ≥15 years old and it was 0, 17/100,000 in men and 0, 21/100,000 in women. Overall survival was 39 months. According to the IPSS score, the high-risk forms are low and their overall survival was 15 months. The rate of transformation into acute myeloid leukaemia (AML) is 32%. Conclusion: This national epidemiological survey shows an annual progression rate of 19% and an increase in incidence from 0.007/100.000 in 2005 to 0.45/100.000 in 2019.
Homogenization Modeling of Periodically Wrapped String-Harnessed Beam Structures: Experimental Validation

High-flexibility, small-mass, and high-bandwidth controllers required for inflatable space missions need quite accurate models to predict higher-order dynamics of these structures. Some major components often ignored in dynamic analysis of space structures that result in model inaccuracies are space flight cables. The cables-to-payload-mass ratio can be about 20% in a traditional space structure; this number can increase significantly for inflatable structures due to their extremely light weights. As such, these cables can have significant impacts on the structural dynamics. The presented paper considers string-harnessed beam structures as a way to study the mass and stiffness effects of these cables. As a preliminary step, damping is ignored in the presented models. A homogenization technique is applied to develop the governing partial differential equation for the transverse bending coordinate of vibration of the string-harnessed beam structures. A periodic wrapping pattern is considered for the string .
Exploring the Perspectives of Young Adults on Cervical Cancer Screening in the Western Region of Ghana: A Qualitative Enquiry

Background: Cervical cancer is a major public health issue globally. In Ghana, there is paltry of information on the perspectives of young adults on cervical cancer. Aim: The study explored the perception of young adults between the ages of 17-24 on cervical cancer screening at a hospital in the Sekondi-Takoradi Metropolis of the western region of Ghana. Methods: Qualitative exploratory descriptive method was employed in this study. 13 participants who met the inclusion criteria were purposively selected for the study. Respondents were interviewed face-to-face using a semi structured interview guide designed based on the research objectives. All interviews were audio taped, transcribed verbatim and analysed using thematic content analysis. Results: Younger adults had adequate knowledge on cervical cancer but inadequate information on its screening methods. All young adults in the study had never done screening because they did not see themselves susceptible to the development of cervical cancer and some opined that, cervical cancer is a disease for older and pregnant women. Conclusion: Participants had an incorrect perception on cervical cancer screening so more education should be put in place in our institutions to increase their knowledge on the dangers of cervical cancer and the screening methods available in its prevention.
Public and private transportation in Chinese cities: impacts of population size, city wealth, urban typology, the built environment, and fuel price

The development of urban transportation is affected by city population size, wealth, urban typology, the built environment, and fuel price, and has significant implications for urban sustainability. We analyze data of 297 Chinese cities between 2017 and 2019 using both simple regressions to examine the relationships between metrics of public and private transportation and city size, and multiple regressions to examine the impacts of the above urban factors on public transit use and private vehicle number. Both public transit use and private vehicle number scale super-linearly with population and sub-linearly with gross regional product. We find that the impacts of population size, city wealth, the built environment, and fuel price on transportation vary across city groups (industrial, mixed-economy, and commercial cities). We find that the relationships between urban transportation metrics and their factors extracted from intra-city variations over time are different from those derived from pooling data of multiple cities over time, indicating the importance of choosing appropriate analyses to inform local policymaking. A key finding is that to reduce private vehicle ownership, enhancing land use diversity, increasing rail transit, and expanding taxi fleets are more effective than increasing density in already dense Chinese cities. Our findings improve understanding of the drivers of public and private transportation in urban China which are needed to promote sustainable growth of Chinese cities.
Costing analysis of an information & communications technology-enabled primary healthcare facility in India

Background & objectives: Information and communications technology (ICT) has often been endorsed as an effective tool to improve primary healthcare. However, evidence on the cost of ICT-enabled primary health centre (PHC) is lacking. The present study aimed at estimating the costs for customization and implementation of an integrated health information system for primary healthcare at a public sector urban primary healthcare facility in Chandigarh. Methods: We undertook economic costing of an ICT-enabled PHC based on health system perspective and bottom-up costing. All the resources used for the provision of ICT-enabled PHC, capital and recurrent, were identified, measured and valued. The capital items were annualized over their estimated life using a discount rate of 3 per cent. A sensitivity analysis was undertaken to assess the effect of parameter uncertainties. Finally, we assessed the cost of scaling up ICT-enabled PHC at the state level. Results: The estimated overall annual cost of delivering health services through PHC in the public sector was ₹ 7.88 million. The additional economic cost of ICT was ₹ 1.39 million i.e. 17.7 per cent over and above a non-ICT PHC cost. In a PHC with ICT, the cost per capita increased by ₹ 56. On scaling up to the state level (with 400 PHCs), the economic cost of ICT was estimated to be ₹ 0.47 million per year per PHC, which equates to approximately six per cent expenditure over and above the economic cost of a regular PHC. Interpretation & conclusions: Implementing a model of information technology-PHC in a state of India would require an augmentation of cost by about six per cent, which seems fiscally sustainable. However, contextual factors related to the availability of infrastructure, human resources and medical supplies for delivering quality PHC services will also need to be considered.