Miracle-dz/UniFAR-FTUs huggingface dataset:
Maternal Experiences of Parenting Young Children While Battling Breast Cancer in Taiwan

Background Western mothers with breast cancer find themselves struggling with parenting responsibilities. Because parenting is culturally shaped, literature on ill mothers' experiences of parenting young children from a collectivist culture is limited. Objective The aim of this study was to examine Taiwanese mothers' experiences of and cultural practices embedded in parenting young children while in treatment for breast cancer. Methods Sixteen mothers with at least 1 child younger than 12 years were recruited from a breast cancer organization in Taiwan. Data were collected from November 2016 to June 2017 via a semistructured individual interview and analyzed using a content analysis technique. Results Five themes, namely, “maternal limited disclosure, complementary with children's tacit knowledge,” “the scar is no longer a scar but a symbol for intimate bonding,” “issues of maternal absence for young children and school-aged children,” “the power of ‘We are a family’,” and “to live a simple life and to live for one's self,” elucidate how cultural beliefs and practices shape maternal parenting experiences during treatment of breast cancer. Conclusions The findings illustrate how Taiwanese mothers interpret their illness, contextualize illness-related messages in daily life, and maintain family ties while coping with breast cancer. The will to maintain the family's integrity, including the extended family, can empower mothers throughout the trajectory. Implications for Practice Ill mothers may benefit from nurses' guidance on daily routines and cultural practices that they can exploit to frame illness messages for their children. Maternal efforts to change their outlook on life, vocalize their needs, and become more assertive should be acknowledged and supported.
SUMOylation Is Required for ERK5 Nuclear Translocation and ERK5-Mediated Cancer Cell Proliferation

The MAP kinase ERK5 contains an N-terminal kinase domain and a unique C-terminal tail including a nuclear localization signal and a transcriptional activation domain. ERK5 is activated in response to growth factors and stresses and regulates transcription at the nucleus by either phosphorylation or interaction with transcription factors. MEK5-ERK5 pathway plays an important role regulating cancer cell proliferation and survival. Therefore, it is important to define the precise molecular mechanisms implicated in ERK5 nucleo-cytoplasmic shuttling. We previously described that the molecular chaperone Hsp90 stabilizes and anchors ERK5 at the cytosol and that ERK5 nuclear shuttling requires Hsp90 dissociation. Here, we show that MEK5 or overexpression of Cdc37—mechanisms that increase nuclear ERK5—induced ERK5 Small Ubiquitin-related Modifier (SUMO)-2 modification at residues Lys6/Lys22 in cancer cells. Furthermore, mutation of these SUMO sites abolished the ability of ERK5 to translocate to the nucleus and to promote prostatic cancer PC-3 cell proliferation. We also show that overexpression of the SUMO protease SENP2 completely abolished endogenous ERK5 nuclear localization in response to epidermal growth factor (EGF) stimulation. These results allow us to propose a more precise mechanism: in response to MEK5 activation, ERK5 SUMOylation favors the dissociation of Hsp90 from the complex, allowing ERK5 nuclear shuttling and activation of the transcription.
BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses

Chromatin dynamics regulated by epigenetic modification is crucial in genome stability and gene expression. Various epigenetic mechanisms have been identified in the pathogenesis of human diseases. Here, we examined the effects of ten epigenetic agents on pseudorabies virus (PRV) infection by using GFP-reporter assays. Inhibitors of bromodomain protein 4 (BRD4), which receives much more attention in cancer than viral infection, was found to exhibit substantial anti-viral activity against PRV as well as a range of DNA and RNA viruses. We further demonstrated that BRD4 inhibition boosted a robust innate immune response. BRD4 inhibition also de-compacted chromatin structure and induced the DNA damage response, thereby triggering the activation of cGAS-mediated innate immunity and increasing host resistance to viral infection both in vitro and in vivo. Mechanistically, the inhibitory effect of BRD4 inhibition on viral infection was mainly attributed to the attenuation of viral attachment. Our findings reveal a unique mechanism through which BRD4 inhibition restrains viral infection and points to its potent therapeutic value for viral infectious diseases.
Infigratinib in upper tract urothelial carcinoma versus urothelial carcinoma of the bladder and its association with comprehensive genomic profiling and/or cell‐free DNA results

Infigratinib (BGJ398) is a potent and selective fibroblast grown factor receptor 1 to 3 (FGFR1‐3) inhibitor with significant activity in patients with advanced or metastatic urothelial carcinoma bearing FGFR3 alterations. Given the distinct biologic characteristics of upper tract urothelial carcinoma (UTUC) and urothelial carcinoma of the bladder (UCB), the authors examined whether infigratinib had varying activity in these settings.
The complete mitochondrial genome of Flavoperla biocellata Chu, 1929 (Plecoptera: Perlidae) and the phylogenetic analyses of Plecoptera

Of the roughly 400 species of Perlidae in the world, most species are widely distributed in the northern hemisphere, but a few can be found in South Africa and South America. There are only five species in the genus Flavoperla of the family Perlidae in China. To gain a better understanding of the architecture and evolution of mitochondrial genome in Flavoperla, the entire mitochondrial genome (mitogenome) of a Chinese Flavoperla biocellata Chu, 1929 from family Perlidae (Insecta: Plecoptera) was sequenced. The 15,805-bp long mitochondrial genome of F. biocellata contained 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a putative control region (CR). The gene arrangement of F. biocellata was identical with that of other stoneflies and with the fly Drosophila yakuba. Most PCGs of F. biocellata used the standard ATN start codons and complete TAN termination codons. Twenty-one of the 22 tRNA genes exhibited cloverleaf secondary structures, but the dihydrouridine (DHU) arm of trnSer (AGN) was completely reduced. Phylogenetic analyses with both Bayesian inference (BI) and maximum likelihood methods (ML) generated similar topology, both supporting the monophyly of all stonefly families and the infraorder Systellognatha. The phylogenetic analysis based on mitochondrial genomic data from 30 stonefly species recovered a well-supported tree resolving higher-level relationships within Plecoptera. The northern hemisphere suborder Arctoperlaria divided into two groups, Euholognatha and Systellognatha. The southern hemisphere suborder Antarctoperlaria formed two clades: Eustheniidae+Diamphipnoidae and Austroperlidae+ Gripopterygidae; consistent with relationships proposed based on morphology. The final relationships within Plecoptera were recovered as (((Perlidae+(Perlodidae+Chloroperlidae))+(Pteronarcyidae+(Peltoperlidae+Styloperlidae))) +(Taeniopterygidae+(Capniidae+(Nemouridae+Notonemouridae))))+ (Gripopterygoidae+Eusthenioidae).
Factorial Structure and Validity of Depression (PHQ-9) and Anxiety (GAD-7) Scales after Traumatic Brain Injury

Background: The dimensionality of depression and anxiety instruments have recently been a source of controversy. Objectives and Design: In a European-wide sample of patients after Traumatic Brain Injury (TBI), we aim to examine the factorial structure, validity, and association of the Patient Health Questionnaire for depression (PHQ-9) and the Generalized Anxiety Disorder (GAD-7) instruments. This study is based on longitudinal observational data. We conducted analyses of factorial structure and discriminant validity of outcomes six-months after TBI. We also examined the prevalence, co-occurrence, and changes of scores on the PHQ-9 and GAD-7 at 3-, 6-, and 12-month post-TBI assessments. Participants: At six-months post-TBI assessment, 2137 (738 (34.5%) women) participants completed the PHQ-9 and GAD-7 questionnaires. For the longitudinal analysis, we had 1922 participants (672 (35.0%) women). Results: The results of exploratory factor analysis suggested a general latent construct underlying both PHQ-9 and GAD-7 measures. Confirmatory factor analyses showed a slight improvement in the fit indices for the bifactorial model. The Omega hierarchical test clearly differentiated two subfactors of PHQ-9 and GAD-7 items over and above the underlying general factor; however, most of the variance (85.0%) was explained by the general factor and the explained variance of the subfactors was small. The PHQ-9 and GAD-7 performed similarly in detecting post-traumatic stress disorder (PTSD). As defined by conventional cut-offs, depression and anxiety have different prevalence rates in the sample. The scales also differed in their relationships with the short form of health survey (SF-36v2) subscales. The longitudinal analysis showed high stability of depression and anxiety symptoms: 49–67% of the post-TBI patients with comorbid depression and anxiety reported the persistence of the symptoms over time. Discussion: The factorial structure analysis favors a general latent construct underlying both depression and anxiety scales among patients after TBI. We discuss the implications our findings and future research directions.
Inguinal Endometriosis: An Unusual Cause of Groin Pain

Medicine, The University of Tokyo, Tokyo, Japan; Department of Internal Medicine, Suwa Central Hospital, Nagano, Japan Phone: +81 3 5841 3480 e-mail: hirohisa.fujikawa@gmail.com ORCID: orcid.org/0000-0002-8195-1267 Received: 20 February 2020 Accepted: 26 March 2020 • DOI: 10.4274/balkanmedj.galenos. 2020.2020. 2.105 Available at www.balkanmedicaljournal. org Cite this article as: Fujikawa H, Uehara Y. Inguinal Endometriosis: An Unusual Cause of Groin Pain. Balkan Med J 2020;37:291-2 ©Copyright 2020 by Trakya University Faculty of Medicine / The Balkan Medical Journal published by Galenos Publishing House. Balkan Med J 2020;37:291-2 Clinical Image 291
Gender Identity and Assignment Recommendations in Disorders of Sex Development Patients: 20 Years’ Experience and Challenges

Objective: Gender assignment in infants and children with disorders of sex development (DSD) is a stressful situation for both patient/families and medical professionals. Methods: The purpose of this study was to investigate the results of gender assignment recommendations in children with DSD in our clinic from 1999 through 2019. Results: The mean age of the 226 patients with DSD at the time of first admission were 3.05±4.70 years. 50.9% of patients were 46,XY DSD, 42.9% were 46,XX DSD and 6.2% were sex chromosome DSD. Congenital adrenal hyperplasia (majority of patients had 21-hydroxylase deficiency) was the most common etiological cause of 46,XX DSD. In 46,XX patients, 87 of 99 (89.7%) were recommended to be supported as a female, 6 as a male, and 4 were followed up. In 46,XY patients, 40 of 115 (34.8%) were recommended to be supported as a female, and 70 as male (60.9%), and 5 were followed up. In sex chromosome DSD patients, 3 of 14 were recommended to be supported as a female, 9 as a male. The greatest difficulty in making gender assignment recommendations were in the 46,XY DSD group. Conclusion: In DSD gender assignment recommendations, the etiologic diagnosis, psychiatric gender orientation, expectation of the family, phallus length and Prader stage were effective in the gender assignment in DSD cases, especially the first two criteria. It is important to share these experiences among the medical professionals who are routinely charged with this difficult task in multidisciplinary councils.
Upper gastrointestinal endoscopy findings in patient presenting with dyspepsia

ntroductions : The objective of this study was to evaluate the upper gastrointestinal endoscopy findings in patients presenting with dyspepsia. Methods : This retrospective observational study was conducted in Department of internal medicine, Patan Hospital from April 2013 to March 2014. Adult patients who underwent upper gastrointestinal endoscopy for dyspepsia were included in the study. Results : There were 2141 endoscopies (out of total 3195) performed for dyspepsia, male 996 (46.52 %), female 1145 (53.48%), mean age 39.37 years (SD ±18.16). A single endoscopic diagnosis was made in 1991 (93%) and in rest combinations of lesions were seen. Gastritis 892 (41.66%), Oesophagitis 215 (10.04%), Duodenal Ulcer 100 (4.67%), Gastro-duodenitis 85 (3.97%), Hiatus hernia 82 (3.82%), Gastric Ulcer 46 (2.14%) and no lesions in 594 (27.74%) were seen. Conclusion : Gastritis followed by oesophagitis was seen half of the dyspeptic, while a quarter had functional dyspepsia with normal findings. Keywords : duodenal ulcer, gastritis, gastric ulcer, hiatus hernia, oesophagitis, upper gastrointestinal (UGI) endoscopy
Epidemiological and Clinical Predictors of COVID-19

Abstract Background Rapid identification of COVID-19 cases, which is crucial to outbreak containment efforts, is challenging due to the lack of pathognomonic symptoms and in settings with limited capacity for specialized nucleic acid–based reverse transcription polymerase chain reaction (PCR) testing. Methods This retrospective case-control study involves subjects (7–98 years) presenting at the designated national outbreak screening center and tertiary care hospital in Singapore for SARS-CoV-2 testing from 26 January to 16 February 2020. COVID-19 status was confirmed by PCR testing of sputum, nasopharyngeal swabs, or throat swabs. Demographic, clinical, laboratory, and exposure-risk variables ascertainable at presentation were analyzed to develop an algorithm for estimating the risk of COVID-19. Model development used Akaike’s information criterion in a stepwise fashion to build logistic regression models, which were then translated into prediction scores. Performance was measured using receiver operating characteristic curves, adjusting for overconfidence using leave-one-out cross-validation. Results The study population included 788 subjects, of whom 54 (6.9%) were SARS-CoV-2 positive and 734 (93.1%) were SARS-CoV-2 negative. The median age was 34 years, and 407 (51.7%) were female. Using leave-one-out cross-validation, all the models incorporating clinical tests (models 1, 2, and 3) performed well with areas under the receiver operating characteristic curve (AUCs) of 0.91, 0.88, and 0.88, respectively. In comparison, model 4 had an AUC of 0.65. Conclusions Rapidly ascertainable clinical and laboratory data could identify individuals at high risk of COVID-19 and enable prioritization of PCR testing and containment efforts. Basic laboratory test results were crucial to prediction models.
Gentisic Acid Stimulates Keratinocyte Proliferation through ERK1/2 Phosphorylation

Keratinocyte proliferation is important for skin wound healing. The wound healing process includes blood clotting around the wound, removal of dead cells and pathogens through inflammation, and then re-epithelialization through proliferation and maturation. Proliferation assay was performed on acid natural compounds to identify candidates for natural-derived components of skin injury treatment. We found that gentisic acid promoted high cell proliferation activity compared with other compounds. Gentisic acid improved HaCaT cell proliferation by over 20% in MTT assay. Gentisic acid also had higher healing activity in an in vitro wound healing assay than allantoin as a positive control. Furthermore, we have identified how the treatment of gentisic acid can increase proliferation in the cell. Western blot analysis of proteins in the mitogen-activated protein (MAP) kinase signaling pathway showed that ERK1/2 phosphorylation was increased by gentisic acid treatment. Thus, our study indicates that gentisic acid promotes the proliferation of keratinocyte by phosphorylation of ERK1/2.
Thermal Convection of Nanoliquid in a Double-Connected Chamber

Thermogravitational convective thermal transmission, inside a square differentially-heated chamber with a nanoliquid, has been examined in the presence of internal adiabatic or a thermally-conducting solid body. A single-phase nanoliquid approach is employed, based on the experimentally-extracted relations for nanofluid heat conductivity and dynamic viscosity. The governing equations have been written using non-primitive parameters such as stream function and vorticity. Such approach allows a decrease in computational time due to a reduction of equation numbers. One of the main challenges in such a technique is a determining the stream function magnitude at the inner body walls. A solution of this problem has been described in detail in this paper. Computational scrutinizing has been performed by employing the finite difference technique. The mesh sensitivity analysis and comparison with theoretical and experimental results of other researchers have been included. An influence of the Rayleigh number, nanoparticles concentration, internal block size, heat conductivity ratio and non-dimensional time on nanofluid motion and energy transport has been studied.
MiR-483 Targeted SOX3 to Suppress Glioma Cell Migration, Invasion and Promote Cell Apoptosis

Objective Glioma is the most common malignant brain tumor that has high aggressiveness. The aim of this study was to investigate the potential therapeutic targets for gliomas. Materials and Methods Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to calculate the expression of miRNA and genes. The connection between the expression of miR-483 and patients’ overall survival rate was evaluated using Kaplan–Meier analysis. In addition, the underlying mechanism was detected using luciferase assay. Results The expression level of miR-483 was significantly decreased in glioma tissue samples and cell lines, compared to the adjacent tissues and normal cell lines. Downregulation of miR-483 or upregulation of SOX3 was associated with overall survival of glioma patients. Additionally, overexpression of miR-483 promotes cell invasion and migration and inhibits apoptosis. In addition, miR-483 directly targeted to SOX3, and the expression of miR-483 has a negative correlation with SOX3 in glioma tissues. SOX3 reversed partial functions of miR-483 on cell migration, invasion, and promoted cell apoptosis in glioma. Conclusion MiR-483 inhibited glioma cell migration, invasion, and promoted glioma cell apoptosis by targeting SOX3. MiR-483 maybe acted as a potential target for the treatment of glioma.
Predisposition to Change Is Linked to Job Satisfaction: Assessing the Mediation Roles of Workplace Relation Civility and Insight

The globalization processes typical of liquid modern society require organizations to have high levels of flexibility, dynamism, and rapidity of change, testing the adaptability of workers with possible repercussions on well-being and productivity. Therefore, this study aimed to investigate the role of several psychological factors in favoring job satisfaction in a group of organizational workers (mean age = 46.24; SD = 9.99; 40.9% males and 59.1% females). Firstly, the impact of predisposition to change on job satisfaction through workplace relational civility (others with me) or insight orientation as independent mediating variables was analyzed. After that, this relationship was also studied by testing the effect that the simultaneous interaction of both mediators could have. Results show that workplace relational civility (others with me) significantly mediated the relationship between predisposition to change and job satisfaction, while no significance was found in the effect of insight when considered individually. However, the latter acquires greater relevance if placed in interaction with the other mediator, that is found to be the most proximal factor linking job satisfaction to the other more distal variables. Such findings might have a relevant role in strengthening preventive intervening, favoring positive results for greater well-being of both subjects and organizations.
Higher Frequency of Transfusion-Transmitted Virus (TTV) in HIV Patients in Comparison with Healthy Blood Donors

Background: Epidemiologicalstudieshavereportedcommonlydistributedtransfusion-transmittedvirus(TTV)indiﬀerentpopu-lations with parental risk factors, including human immunodeﬁciency virus (HIV). Objectives: This study was performed to determine and compare the prevalence of TTV infection among HIV-positive patients and healthy blood donors. Patients and Methods: A total of 186 HIV patients and 165 healthy blood donors with no markers of HIV infection were included in this study during 2004 - 2012. Semi-nested Polymerase Chain Reaction (PCR) assay was performed for the detection of TTV DNA. HBV surface antigen (HBsAg), Hepatitis C Virus Antibody (HCVAb), and CD4 were investigated in the sera of HIV-positive subjects. Aspartate transaminase (AST) and Alanine transaminase (ALT) levels were also measured. Results: The mean age of HIV-positive and healthy subjects was 39.3 and 40.5 years, respectively. In total, 182 (97.9%) subjects were male and 4 (2.1%) were female. TTV DNA was detected in 35 out of 186 HIV patients (18.8%; 95% CI, 13.2 - 24.4%). The prevalence of TTV in the HIV group was signiﬁcantly higher (P = 0.027) than blood donors (11%; 95% CI, 6.2 - 15.8%). Age, marital status, unsafe sexual activity, and use of injection drugs were not signiﬁcantly associated with the prevalence of TTV infection in HIV patients. Conclusions: Considering the higher frequency of TTV infection in HIV patients in comparison to healthy blood donors, HIV infection may be an important risk factor for TTV infection. In addition, the lower frequency of TTV infection in healthy individuals in comparison to HIV patients reveals the transmission of TTV infection via routes other than blood and drug injection; therefore, the eﬀect of fecal-oral route needs to be examined in future studies.
Glucocorticoid-Induced Osteoporosis Management Among Seniors

Background: Chronic oral glucocorticoid (GC) therapy is the le ading cause of secondary osteoporosis, resulting in rapid bone loss and incr eased fracture risk. Objective: To examine trends in GC-induced osteoporosis management, by se x and over time. Methods: Using Ontario healthcare utilization data, we determined the prop o tion of chronic oral GC users (age ≥66) who received a bone mineral density test and/or osteopo rosis treatment within 6 months of starting chronic GC therapy. Results: We identified 72,099 male and 95,975 female patien ts o chronic GC therapy between 1996 and 2012. Overall, 15% of m en and 36% of women on chronic oral GC therapy received osteoporosis management. Howeve r, among patients without prior osteoporosis management, only 12% of men and 23% of women received osteoporosis management within 6 months. Conclusion: GC-induced osteoporosis management improved significantly over time in both sexes yet remains l ow, particularly among men. This represents a missed opportunity for fracture prevention among pa tients requiring prolonged GC therapy.