Miracle-dz/UniFAR-FTUs huggingface dataset:
Genomics of heifer pregnancy, days open, and days to conception in Red Angus heifers

Although it is challenging to collect information on reproductive traits, fertility traits are believed to play a significant role in the growth of the beef industry and bring revenue to the producers. It has been challenging to make genetic improvement of fertility traits because of scarce records and their low heritability. The joint analyses of economic and indicator traits provided much information for the analysis of other production traits, resulting in favorable improvement. The same method can be applied to reproductive traits to increase prediction accuracy. Furthermore, the ubiquity of high-throughput genotypes and the development of advanced computational methods give hope that there is a possibility of determining the genetic influence on fertility traits. The advancement in phenotypic collection technology eases the keeping of records on both economic and indicator fertility traits. Also, it allows the development of new traits to be analyzed while reducing the cost of data availability. Heifer Pregnancy (HP) - the ability for a heifer to conceive by the end of the breeding season; Days Open (DO) - days of breeding season a heifer remained open; and Days to Conception (DC) -- the number of days it took for a heifer to get pregnant, are easy to measure fertility traits with enough information to make beef reproductive genetic improvement in the industry. The objective of the current study is to identify DNA markers tagging genes influencing HP, DC, and DC among 18,039 Red Angus heifers, find the genetic relationship among those traits, and creating the genomic predictions of days to conception and days open. The results show a small heritability of 0.119 to 0.131, 0.10 to 0.102, and 0.0749 to 0.112 for HP, DO, and DC, respectively. The DC model resulted in higher accuracy than other models. There was a high correlation between HP and DO (r = - 0.61 and 0.85 from the linear model and the liability scale model, respectively). The de-regressed estimated breeding value (EBV) genomewide association (GWAS) yielded 58 and 2 significant SNPs at suggestive significant level (pvalue [less than] 1.0e-05) for HP and DC, respectively. This study found GRID2 and ZMIZ1 genes to be associated with heifer pregnancy, and it has been speculated that the central nervous system related genes ontology and the hormones it controls might suggest the physiology behind some of the reproductive differences. The present study confirms the low heritability status of fertility traits and shows the possibilities of genetic enhancement based on the obtained accuracies. The identified genes and gene terms will serve as starting points for future studies that might focus on different phenotypes while reducing the cost of phenotyping and improving the accuracy of fertility traits genomic predictions.
Extraction of Bioactive Compounds for Antioxidant, Antimicrobial, and Antidiabetic Applications

This study was designed to check the potential of secondary metabolites of the selected plants; Citrullus colocynthis, Solanum nigrum, Solanum surattense, Calotropis procera, Agave americana, and Anagallis arvensis for antioxidant, antibacterial, antifungal, and antidiabetic agents. Plant material was soaked in ethanol/methanol to get the crude extract, which was further partitioned via solvent extraction technique. GCMS and FTIR analytical techniques were applied to check the compounds responsible for causing antioxidant, antimicrobial, and antidiabetic activities. It was concluded that about 80% of studied extracts/fractions were active against α-amylase, ranging from 43 to 96%. The highest activity (96.63%) was exhibited by butanol fractions of A. arvensis while the least response (43.65%) was shown by the aqueous fraction of C. colocynthis and the methanol fraction of fruit of S. surattense. The highest antioxidant activity was shown by the ethyl acetate fraction of Anagallis arvensis (78.1%), while aqueous as well as n-hexane fractions are the least active throughout the assay. Results showed that all tested plants can be an excellent source of natural products with potential antimicrobial, antioxidant, and antidiabetic potential. The biological response of these species is depicted as a good therapeutic agent, and, in the future, it can be encapsulated for drug discovery.
Depressive Symptom Trajectories and Cognition Among Older American Couples: A Dyadic Perspective

Objectives This study examined whether trajectories of depressive symptoms of one spouse are associated with the other spouse’s memory. Methods Longitudinal data from the Health and Retirement Study (2004–2016) were used (N = 5690 heterosexual couples). Latent-class growth analysis and structural equation models examined the actor and partner effects of depressive symptom trajectories on memory. Results Four depressive symptom trajectories were identified (i.e., persistently low, increasing, decreasing, and persistently high). Compared to the low trajectory group, the increasing and persistently high trajectories were associated with worse memory for both men and women. While none of the wives’ depressive symptom trajectories was significantly associated with husbands’ memory (p > .05), husbands’ decreasing trajectory was linked to wives’ better memory (β = 0.498, 95% CI = 0.106, 0.890). Discussion Older adults with increasing and persistently high depressive symptoms may experience worse memory. Psychosocial interventions targeting depressive symptoms among older men may be beneficial to their spouses’ memory.
POTENTIALITIES OF SOME OILSEED PROTEIN PRODUCTS AS NITROGEN SOURCES IN CULTURE MEDIA FOR THE PRODUCTION OF RENNIN-LIKE ENZYME FROM Rhizomucor pusillus 1653

A comparative study on different culture media for the production of a rennin-like enzyme from Rhizomucor pusillus 1653 was carried out for preparation of an economic medium to be used for production of rennin like enzyme from Rhizomucor pusillus 1653 . The investigated media were prepared mainly from whey permeate supplemented with oilseed meals as sources of nitrogen. The oilseed meals included: sesame meal, sunflower meal, peanut meal, cottonseed meal, as well as two degossypolized cottonseed meals. Results, revealed that the ratio of milk clotting activity (MCA) to proteolytic activity (PA) was taken as an index for the selection between different media. On this basis results showed that the milk clotting enzyme from Rhizomucor pusillus 1653 was produced by all the media supplemented with oilseed meals in the following order: Sesame meal > sunflower meal > cottonseed meal (A:H:W)>cottonseed meal (acetic: ether)>cottonseed meal > peanut meal. The activity of milk clotting enzyme resulting from whey permeate supplemented with sesame meal decreased rapidly as heat treatment increased over 55  C and pH 6.0. Furthermore, the enzyme activity decreased gradually as Nacl concentration increased but was improved greatly by increasing the calcium ion concentration. Utilization of the Rhizomucor pusillus rennet proved to be compatible with calf rennet for the preparation of Ras cheese slurry.
Recombinant Human Collagen-Based Bioinks for the 3D Bioprinting of Full-thickness Human Skin Equivalent

As a major extracellular matrix component within the skin, collagen has been widely used to engineer human skin tissues. However, most collagen is extracted from animals. Here, we introduced recombinant human type III collagen (rhCol3) as a bioactive component to formulate bioinks for the bioprinting of a full-thickness human skin equivalent. Human dermal fibroblasts were encapsulated in the gelatin methacryloyl-rhCol3 composite bioinks and printed on a transwell to form the dermis layer, on which human epidermal keratinocytes were seeded to perform an air-liquid interface culture for 6 weeks. After optimizing the bioink formulation and bioprinting process, we investigated the effect of rhCol3 on skin tissue formation. The results suggest that a higher concentration of rhCol3 would enhance the growth of both cells, resulting in a more confluent (~100%) spreading of the epidermal keratinocytes at an early stage (3 days), compared to the rhCol3-free counterpart. Moreover, in an in vivo experiment, adding rhCol3 in the hydrogel formulation would contribute to the skin wound healing process. Taken together, we conclude that rhCol3 could act as a functional bioink component to promote basic skin cellular processes for skin tissue engineering.
Intermediate beta-shifts of finite type

A bstract . An aim of this article is to highlight dynamical di ﬀ erences between the greedy, and hence the lazy, β -shift (transformation) and an intermediate β -shift (transformation), for a ﬁxed β ∈ (1 , 2). Speciﬁcally, a classiﬁcation in terms of the kneading invariants of the linear maps T β,α : x (cid:55)→ β x + α mod 1 for which the corresponding intermediate β -shift is of ﬁnite type is given. This characterisation is then employed to construct a class of pairs ( β,α ) such that the intermediate β -shift associated with T β,α is a subshift of ﬁnite type and where the map T β,α is not transitive. This is in contrast to the situation for the corresponding greedy and lazy β -shifts and β -transformations, in that these two properties do not hold.
Plant fossils from Arung Khola and Binai Khola formations of Churia Group (Siwalik), west central Nepal and their palaeoecological and phytogeographical significance

Systematic study of plant megafossils comprising dicotyledonous leaves and seeds collected from the Arung Khola and Binai Khola formations of the Churia Group, exposed in Tinau Khola and Mahendra Highway between Barghat and Dumkibas, Nepal, has revealed 15 taxa out of which 14 are new belonging to 14 genera of 12 families. They are named as Orophea siwalika sp. nov., Miliusa brochidodroma sp. nov. (Annonaceae); Gynocardia butwalensis sp. nov. (Flacourtiaceae); Shorea miocenica sp. nov., S. nepalensis sp. nov., Hopea siwalika Antal & Awasthi (Dipterocarpaceae); Grewia mallotophylla sp. nov. (Tiliaceae); Chisocheton ellipticus sp. nov., Ventilago ovatus sp. nov. (Rubiaceae), Swintonia butwalensis sp. nov. (Anacardiaceae); Mitragyna tertiara sp. nov., Mussaendopsis suborbiculatus sp. nov. (Rubiaceae); Alangium nepalensis sp. nov. (Alangiaceae); Homonoia lanceolata sp. nov. (Euphorbiaceae) and Ficus miocenicus sp. nov. (Moraceae). The modern counterparts of these fossils are mostly distributed in the tropical evergreen to semi-evergreen forests of Indo-Malayan region which indicate the existence of similar types of forests in the frontal Himalayan foot-hill zone during the Middle Miocene-Pliocene. The absence of tropical evergreen dipterocarps and their associates in the present-day flora of this region reflects changes in the annual mean temperature and rainfall caused by further uplift of the Himalayas and the northward movement of the Indian Plate.
A COMPARATIVE STUDY BETWEEN TWO APRICOT CULTIVARS IN RELATION WITH PROTEIN PROFILE

The two tested apricot cultivars (Amal and Canino) which grown under Giza conditions were compared, for their tree growth, fruiting, yield as well as fruit quality and protein profile. Results showed that the chilling requirements for Canino trees are higher than there for Amal apricot cultivar. The shoot length and leaf area were greater for Amal trees than Canino. In both cultivars the majority of the fruits were on one year old shoots. The fruit weight and size were greater for Canino than Amal. Consequently, the yield in killograms per tree was higher for Canino than Amal. The other fruit characters were highly similar. The protein profile indicated a close genetic relationship between the two caltivars. The total number of bands for each cultivar is eleven, eight of them are the same for the two cultivars.
Mutations in Exons 8 and 11 of c-kit Gene in Canine Subcutaneous Mast Cell Tumors and Their Association with Cell Proliferation

Simple Summary Mast cell tumors (MCTs) are one of the most common skin tumors in dogs with variable clinical behavior ranging from benign lesions to those causing widespread metastasis. Prognostic factors have been intensively studied in cutaneous MCTs but are less commonly investigated in subcutaneous MCTs as the majority are benign. Activating mutations in exons 8 and 11 of c-kit, a gene that regulates proliferation and differentiation of mast cells, occur commonly in canine cutaneous MCTs and are strong predictors of prognosis. c-kit mutations have rarely been reported in subcutaneous MCTs. The goal of this study was to identify the prevalence of c-kit mutations in exons 8 and 11 in 216 canine subcutaneous MCTs and to investigate their association with other prognostic factors, including mitotic count, histologic grade, KIT pattern and proliferation markers. We detected c-kit mutations in exons 8 and 11 in 23 (10.6%) and 12 (5.56%) subcutaneous MCTs, respectively. c-kit mutations in exon 11 were associated with histologic high grade and a high mitotic count, suggesting that these parameters can predict the biological behavior of subcutaneous MCTs in a similar manner as in their cutaneous counterparts. Abstract The prognostic significance of internal tandem duplication (ITD) mutations in exons 8 and 11 of c-kit has been well-described for canine cutaneous mast cell tumors (MCTs), but c-kit mutations have rarely been reported in subcutaneous MCTs. The objective of this study was to identify the prevalence of ITD mutations in exons 8 and 11 of c-kit in canine subcutaneous MCTs and to investigate its association with histologic grade, KIT pattern, and proliferation markers. ITD mutations in exons 8 and 11 of c-kit, mitotic count, Ki67 index, AgNOR number, Ki67xAgNOR score, KIT pattern, and histologic grade (two-tier system) were retrospectively recorded for 216 dogs with subcutaneous MCTs. ITD mutations in exons 8 and 11 of c-kit were detected in 23 (10.6%) and 12 (5.56%) subcutaneous MCTs, respectively. Exon 11 mutations were significantly associated with Kiupel high grade (p < 0.001) and increased mitotic count (p < 0.001) compared to subcutaneous MCTs with no mutations in exons 8 or 11 (p = 0.002) or subcutaneous MCTs with a mutation in exon 8 (p = 0.001). There was no significant association of either c-kit mutation with KIT patterns or proliferation activity. This study identified a higher prevalence of ITD mutations in exons 8 and 11 of c-kit in subcutaneous MCTs than previously reported. Like their cutaneous counterpart, subcutaneous MCTs with exon 11 mutations were more likely to be histologically high grade and have a higher mitotic count, whereas such associations were not observed in subcutaneous MCTs with exon 8 mutations.
Prevalence of toxigenic mycobiota and mycotoxins in date palm fruits and investigation on Bacillus cereus 342-2 as biocontrol agent

ABSTRACT Date palm fruit is consumed as a highly nutritious food item particularly in the Middle East. This work was designed to investigate the occurrence of filamentous moulds, their toxins in date palm fruit samples, and explore the biocontrol potential of Bacillus cereus 342-2 against few date palms associated fungal species. In total, 36 date palm fruit samples from different sources and varieties were collected from the markets in Qatar. Aspergillus and Penicillium spp. were found to contaminate most of the samples, except Akhlas samples, which were free of any fungal contamination. In total, ochratoxin A (OTA) was found in 6 (16.67%) date samples, all within the permissible limit, except one (2.7%), where the level was above the maximum permissible concentrations of 2 µg/kg. The volatile organic compounds (VOCs) of B. cereus were very effective against the growth of A. niger and A. carbonarius with an inhibition rate of 100%. Whereas their effects against A. flavus was weak as mycelia grew; however, no sporulation occurred until day 7 of co-incubation. Gas chromatography mass spectrophotometry analysis of B. cereus volatiles showed aldehyde as a major component with a peak area of 10.6%, and Undecane with a peak area of 2.02%. The inhibitory effect of VOCs was either from aldehyde alone, or a synergetic effect of the two molecules.
Commercial Complementary Food in Germany: A 2020 Market Survey

As consumption of commercial complementary food (CCF) during infancy and toddlerhood is common, the aim of the present study was to describe the current (2020) German market of CCF products targeted at infants and toddlers with a special focus on ingredients, macronutrients, and the practice of nutrient fortification. Information on age declarations, ingredients, energy and nutrient contents, and nutrient fortification was obtained in a market survey by contacting the producers and searching manufacturers’ websites. Each product was assigned to 1 of 13 product categories (menus, milk–cereal–meal, fruit–cereal–meal, oil, vegetables, meat, fish, fruits, cereals, snack foods, pouches, desserts, beverages). Descriptive statistics were used. We identified 1057 CF products on the German market (infants’ CCF (<12 months): n = 829; toddlers’ CCF (>12 months): n = 228)). The highest protein content (% of energy content, %E) was found in meat products. In pouches, beverages, cereal fruit meals, and fruits, more than 50% of energy came from total sugar. The highest median salt content was found in toddlers’ menus and desserts. Around one-third of infants’ CCF products and one quarter of toddlers’ products were fortified with nutrients. Vitamin B1 (thiamin) was the most frequently fortified nutrient, followed by vitamin C, iron, calcium, and vitamin D. Apple was the type of fruit listed most often in products with fruits, whereas carrot was the most frequent vegetable among CCF with vegetables. In particular, the high total sugar content of most CCFs currently available on the German market may promote unhealthy dietary habits. Parents need to be advised about the optimal selection of products.
Assessment of quality of life and drug prescription pattern in acute coronary syndrome

Objectives: To assess the health-related quality of life (QOL) in patients with acute coronary syndrome (ACS) and predict those patients who may have worsened QOL 6 months later and also observe the prescribing patterns of drugs given in their treatment. Materials and Methods: A prospective observational study was conducted at the cardiology department in a tertiary care hospital. The data were collected in both inpatient and outpatient cardiology departments based on our inclusion and exclusion criteria for a period of 6 months. A total of 240 patients were analyzed with a data collection form by interviewing the patients about their sociodemographic details, laboratory parameters, and diagnostic reports. The MacNew Heart Disease Questionnaire, Medical Research Council Breathlessness Scale, and New York Heart Association (NYHA) Functional Scale were used for assessing the QOL in patients with ACS. We calculated Global Registry of Acute Coronary Events Score and Thrombolysis in Myocardial Infarction Score for ST-elevation myocardial infarction (STEMI) and non-ST-elevation myocardial infarction (NSTEMI) patients for identifying the mortality risk. Results: Urban people were more prone to ACS than rural people, according to our data. According to the NYHA Functional Classification, ability to do physical activity was more considerably affected in NSTEMI patients than STEMI and angina. Most of the patients had Grade 2 shortness of breath. 35% of the patients had a high mortality risk. Based on the MacNew Questionnaire data, 23% of the patients with ACS were doing emotionally poor, 45% of the patients had shown physically impaired symptoms, 28% of the patients were socially dependent, 8% of the patients showed poor gastric condition. 49% of the patients were given reperfusion therapy with either percutaneous coronary intervention (PCI) or coronary artery bypass graft, 25% of the patients were managed with dual-anticoagulant therapy, and 6% of the patients were treated with single-anticoagulant therapy. Commonly prescribed drug classes were statins (90%), antiplatelets (86%), anticoagulants (75%), antianginal (55%), beta-blockers (50%), diuretics (35%), angiotensin-converting enzyme inhibitors (18%), and angiotensin receptor blockers (16%). Conclusion: QOL was significantly affected in ACS patients. Most of the patients had risk factors for ACS. Patients explained impairments in all the four domains used in the questionnaire such as emotional, physical, social, and gastric impairments. QOL was more affected in STEMI patients and they had a high mortality risk. Most of the patients had NSTEMI. Low-risk patients were given single-anticoagulant therapy and medium-risk patients were treated with dual-anticoagulant therapy. PCI was preferred in almost all the patients.
PARENTAL AWARENESS TOWARDS CHILD HEALTH: A STUDY ON THE PARENTS OF HIGH SCHOOL STUDENTS AT KHULNA CITY CORPORATION IN BANGLADESH

Parents’ awareness regarding child health has a significant influence on physical and psychological issues. This study aims to explore the implication of parents’ behavior and attitude on the mental and physical health status of secondary education level children. A total of 130 respondents (either father or mother) is selected using the snowball sampling technique from Khulna City Corporation (KCC), Bangladesh. This study has applied Principal Component Analysis (PCA) and Ordinary Least Square (OLS) techniques for analyzing data. Results reveal that household structure (joint or nuclear) have significant implications in shaping the physical and mental health status of children. A poor level of parental awareness and care may lead children into depression and impede their mental growth. However, regression results postulate that parents’ awareness and spending more time with children is significantly related to the physical and mental fitness of their children which in turn keeps them on the right track. This study has predominantly explored to what extent parents' awareness matters for improving child physical and mental health status. Thus, parents’ awareness regarding child healthcare issues is indispensable.
From Structural to Functional Hypertension Mediated Target Organ Damage—A Long Way to Heart Failure with Preserved Ejection Fraction

Arterial hypertension (AH) is a major risk factor for the development of heart failure (HF) which represents one of the leading causes of mortality and morbidity worldwide. The chronic hemodynamic overload induced by AH is responsible for different types of functional and morphological adaptation of the cardiovascular system, defined as hypertensive mediated target organ damage (HMOD), whose identification is of fundamental importance for diagnostic and prognostic purposes. Among HMODs, left ventricular hypertrophy (LVH), coronary microvascular dysfunction (CMVD), and subclinical systolic dysfunction have been shown to play a role in the pathogenesis of HF and represent promising therapeutic targets. Furthermore, LVH represents a strong predictor of cardiovascular events in hypertensive patients, influencing per se the development of CMVD and systolic dysfunction. Clinical evidence suggests considering LVH as a diagnostic marker for HF with preserved ejection fraction (HFpEF). Several studies have also shown that microalbuminuria, a parameter of abnormal renal function, is implicated in the development of HFpEF and in predicting the prognosis of patients with HF. The present review highlights recent evidence on the main HMOD, focusing in particular on LVH, CMD, subclinical systolic dysfunction, and microalbuminuria leading to HFpEF.