Miracle-dz/UniFAR-FTUs huggingface dataset:
Upper critical field of NaFe1−xCox as superconductors

The upper critical field of NaFe1−xCoxAs was measured from x=0 to x=0.08, with the magnetic field applied parallel (H∥c2) and normal (H⊥c2) to the planes. The data were fitted using one-band and two-band models. The orbital and paramagnetic components of the upper critical field were extracted for H∥c2, which we find to be strongly dominated by paramagnetic pair breaking. In the parent compound the paramagnetic limit is equal to the value expected by BCS theory. However, substitution of Fe by Co leads to an enhancement above the BCS limit by a factor of approximately 1.6. In the overdoped region, we observe a significant convex curvature in H⊥c2 at low temperatures, which we attribute to the two-band nature of the superconductivity and the asymmetry between the two bands. Additionally, we discuss the behavior of critical field anisotropy, coherence length ξ, and the penetration depth λ.
Trx, redox and M. truncatula

Germination of cereals is accompanied by extensive change in the redox state of seed proteins. Proteins present in oxidized (S-S) form in dry seeds are converted to the reduced (SH) state following imbibition. Thioredoxin (Trx) appears to play a role in this transition in cereals. It is not known, however, whether Trx-linked redox changes are restricted to cereals or whether they take place more broadly in germinating seeds. To gain information on this point, we have investigated a model legume, Medicago truncatula . Two complementary gel-based proteomic approaches were followed to identify Trx targets in seeds: proteins were (i) labeled with a thiol-specific probe, monobromobimane (mBBr), following in vitro reduction by an NADP/Trx system (NTS), or (ii) isolated on a mutant Trx affinity column. Altogether, 111 Trx-linked proteins were identified with few differences between axes and cotyledons. Fifty-nine were new, 34 found previously in cereal or peanut seeds and 18 in other plants or photosynthetic organisms. In parallel, the redox state of proteins assessed in germinating seeds using mBBr revealed that a substantial number of proteins that are oxidized or partly reduced in dry seeds became more reduced upon germination. The patterns were similar for proteins reduced in vivo during germination or in vitro by Trx. In contrast, glutathione and glutaredoxin were less effective as reductants in vitro . Overall, more than half of the potential targets identified with the mBBr labeling procedure were reduced during germination. The results provide evidence that Trx functions in the germination of seeds of dicotyledons as well as monocotyledons. Thiol Modulation of Isolated ATPase and Membrane-Bound ATP Synthase from Spinach by Eschericia coli Thioredoxin. Two Gpx-Like Proteins from Lycopersicon esculentum and Helianthus annuus Are
Characterizing cellular biophysical responses to environmental stresses by relating 1 density, deformability and size 2

17 18 Cellular physical properties are important indicators of specific cell states. Although 19 changes in individual biophysical parameters, such as cell size, density and deformability, 20 during cellular processes have been investigated in great detail, relatively little is known 21 about how they are related. Here, we use a suspended microchannel resonator (SMR) to 22 measure the single-cell density, volume, and passage time through a narrow constriction of 23 populations of cells subjected to a variety of environmental stresses. Osmotic stress 24 significantly affects density and volume, as previously shown. In contrast to density and 25 volume, the effect of an osmotic challenge on passage time is relatively small. Deformability, 26 determined by comparing passage times for cells with similar volume, exhibits a strong 27 dependence on osmolarity, indicating that passage time alone does not always provide a 28 meaningful proxy for deformability. Finally, we find that protein synthesis inhibition, cell 29 cycle arrest, protein kinase inhibition, and cytoskeletal disruption result in unexpected 30 relations between deformability, density, and volume. Taken together, our results suggest 31 that measuring multiple biophysical parameters can detect unique characteristics that 32 more specifically reflect cellular behaviors. 33
PREVALENCE OF CATTLE TICKS AND ACARICIDE EFFICACY TEST IN ARBA MINCH AREAS IN SOUTHWEST, ETHIOPIA

of cattle ticks and acaricide efficacy test in Arba areas in southwest Ethiopia”, the review open oral Abstract Ticks are responsible for blood loss, tick worry, damage to hides, skins and the injection of toxins and transmit a number of pathogenic organisms of animals than any other arthropod vector group. The relevant information on tick species composition and prevalence, and acaricide efficacy are essential to assess tick infestation and to identify the appropriate measure of tick control. Hence, the objective of this study was to assess the prevalence of cattle tick, species composition and evaluate the efficacy of diazinon and amitraz against cattle ticks in Arba Minch areas, southwest Ethiopia. A cross-sectional study was conducted to assess the prevalence and species composition of cattle ticks in Chano village from May to June 2016, whereas the field acaricide efficacy trial was conducted in Shelle Mella village in September 2016. 404 cattle above one-year-old were sampled randomly in Chano village. Ticks were collected and identified into species. The susceptibility status of ticks to diazinon and amitraz was tested in the laboratory. 120 healthy cattle with higher tick infestation were included for diazinon and amitraz efficacy trial. The baseline tick count was done to randomize into control and intervention groups. Thirty cattle were sprayed by diazinon, thirty by amitraz and remaining sixty were used as control (30 for each acaricide). Tick count was done at day-1, day-2, day-3, day-7, day-14 and day-21 of post treatment. Of the 404 cattle examined, 343 were infested at least by one tick and hence, the prevalence was 84.9%. Four genera and nine species of ticks ( Amblyomma variegatum , A. cohaerens, A. lepidum , Hyalomma marginatum rufipes , Boophilus decoloratus , Rhipicephalus pravus , R. praetexatus , R. pulchelus and R. evertsi-evertsi) were documented. A. variegatum was the most prevalent species (71.4%; N = 245), followed by A. cohaerens (6.7%; N = 23). Amitraz and diazinon resulted significant reduction in mean tick count in the field compared to the control. The reduction in tick number due the amitraz was significant up to day-21 post treatment (P = 0.012), while it was up to 14-day for diazinon (P = 0.03). The acaricidal efficacy of amitraz was superior over diazinon as it showed the maximum percentage reduction and had long residual efficacy. Ticks were susceptible to amitraz and diazinon and hence use of the two acaricides is still promising, but may require repeated application. ticks due to amitraz and diazinon acaricides 24 A variegatum A. decoloratus and Rhipicephalus were susceptible to diazinon and amitraz with 100% mortality. The control mortality was observed in A. cohaerens only. The average mortality was 5.6% in control groups.
CMAR_A_241870 1845..1853

Wei Jiang Xiaofei Deng Ting Zhu Yuya Wei Zhen Lei Meimei Guo Jiong Yang 1Department of Ultrasound, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen 518052, People’s Republic of China; 2Department of Gastroenterology, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen 518052, People’s Republic of China Background: Identification of cholangiocarcinoma (CCA) associated with hepatolithiasis (HL) is difficult. There is no effective method to discriminate CCA associated with HL (HLCCA) from HL currently. Objective: To explore the value of clinical data, ultrasonic characteristics and miRNA expression level in the identification of HL-CCA. Methods: Thirty-one patients with HL-CCA in Huazhong University of Science and Technology Union Shenzhen Hospital were enrolled in the observation group, while 40 patients with HL alone were included in the control group. The clinical data, ultrasonic characteristics, and miRNA expression level of the two groups were recorded and analyzed to explore the potential indicators for the identification of HL-CCA. Results: The accuracy of ultrasound in the diagnosis of HL-CCA was low (54.84%). Multivariate logistic regression analysis showed that liver abscess (P=0.021), indistinct border demarcation (P=0.015), non-homogenous echotexture (P=0.019), missed portal vein around lesion (P=0.032), miRNA-21 (P=0.018) and miRNA-221 (P=0.009) were the potential indicators for the identification of HL-CCA. The combined diagnosis based on logistic regression contained liver abscess, border demarcation, echotexture, portal vein around lesion, miRNA-21 and miRNA-221. The results showed that the accuracy of combined diagnosis identifying HL-CCAwas the most accurate (AUC=0.911), which was significantly greater than the AUC of miRNA-21 or miRNA-221 individually (P<0.05), with a sensitivity and specificity of 77.42% and 97.50%, respectively. Conclusion: Patients with HL-CCA show high incidence of hepatic abscess and elevated miRNA-21 and miRNA-221 expression level. The ultrasonic features are more likely to show indistinct border demarcation, non-homogenous echotexture, and missed portal vein around lesion. The combined diagnosis is more accurate in the identification of HL-CCA.
The RNA Binding Protein CsrA Is a Pleiotropic Regulator of the Locus of Enterocyte Effacement Pathogenicity Island of Enteropathogenic Escherichia coli (cid:1)

The a ttaching and e ffacing (A/E) pathogen enteropathogenic Escherichia coli (EPEC) forms characteristic actin-ﬁlled membranous protrusions upon infection of host cells termed pedestals. Here we examine the role of the RNA binding protein CsrA in the expression of virulence genes and proteins that are necessary for pedestal formation. The csrA mutant was defective in forming actin pedestals on epithelial cells and in disrupting transepithelial resistance across polarized epithelial cells. Consistent with reduced pedestal for- mation, secretion of the translocators EspA, EspB, and EspD and the effector Tir was substantially reduced in the csrA mutant. Puriﬁed CsrA speciﬁcally bound to the sepL espADB mRNA leader, and the corresponding transcript levels were reduced in the csrA mutant. In contrast, Tir synthesis was unaffected in the csrA mutant. Reduced secretion of Tir appeared to be in part due to decreased synthesis of EscD, an inner membrane architectural protein of the type III secretion system (TTSS) and EscF, a protein that forms the protruding needle complex of the TTSS. These effects were not mediated through the l ocus of e nterocyte e ffacement (LEE) transcriptional regulator GrlA or Ler. In contrast to the csrA mutant, multicopy expression of csrA repressed transcription from LEE1 , grlRA , LEE2 , LEE5 , escD , and LEE4 , an effect mediated by GrlA and Ler. Consistent with its role in other organisms, CsrA also regulated ﬂagellar motility and glycogen levels. Our ﬁndings suggest that CsrA governs virulence factor expression in an A/E pathogen by regulating mRNAs encoding translocators, effectors, or transcription factors. Bio-Rad ICycler addition to the binding reaction The components of the binding reaction pM TE, 1 (cid:9) binding buffer Tris-HCl KCl), ng/ l dithiothreitol, glycerol, of Superase-in (Ambion), 2 different concentrations of a of were polyacrylamide PhosphorImager PhosphorImager. ImageQuaNT (Molecular used to perform densi- tometric apparent equilibrium dissociation determined two independent experiments the Y Y shift experiments, speciﬁc and ( ﬁnal
Dynamics of Bacterial Swarming The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters

When vegetative bacteria that can swim are grown in a rich medium on an agar surface, they become multinucleate, elongate, synthesize large numbers of flagella, produce wetting agents, and move across the surface in coordinated packs: they swarm. We examined the motion of swarming Escherichia coli , comparing the motion of individual cells to their motion during swimming. Swarming cells’ speeds are comparable to bulk swimming speeds, but very broadly distributed. Their speeds and orientations are correlated over a short distance (several cell lengths), but this correlation is not isotropic. We observe the swirling that is conspicuous in many swarming systems, probably due to increasingly long-lived correlations among cells that associate into groups. The normal run-tumble behavior seen in swimming chemotaxis is largely suppressed, instead, cells are continually reoriented by random jostling by their neighbors, randomizing their directions in a few tenths of a second. At the edge of the swarm, cells often pause, then swim back towards the center of the swarm or along its edge. Local alignment among cells, a necessary condition of many flocking theories, is accomplished by cell body collisions and/or short-range hydrodynamic interactions.
Functional Isoforms of I k B Kinase a (IKK a ) Lacking Leucine Zipper and Helix-Loop-Helix Domains Reveal that IKK a and IKK b Have Different Activation Requirements

The activity of the NF- k B family of transcription factors is regulated principally by phosphorylation and subsequent degradation of their inhibitory I k B subunits. Site-speciﬁc serine phosphorylation of I k Bs by two I k B kinases (IKK a [also known as CHUK] and IKK b ) targets them for proteolysis. IKK a and - b have a unique structure, with an amino-terminal serine-threonine kinase catalytic domain and carboxy-proximal helix-loop-helix (HLH) and leucine zipper-like (LZip) amphipathic a -helical domains. Here, we describe the properties of two novel cellular isoforms of IKK a : IKK a - D H and IKK a - D LH. IKK a - D H and IKK a - D LH are differentially spliced isoforms of the IKK a mRNA lacking its HLH domain and both its LZip and HLH domains, respectively. IKK a is the major RNA species in most murine cells and tissues, except for activated T lymphocytes and the brain, where the alternatively spliced isoforms predominate. Remarkably, IKK a - D H and IKK a - D LH, like IKK a , respond to tumor necrosis factor alpha stimulation to potentiate NF- k B activation in HEK293 cells. A mutant, catalytically inactive form of IKK a blocked IKK a -, IKK a - D H-, and IKK a - D LH-mediated NF- k B activation. Akin to IKK a , its carboxy-terminally truncated isoforms associated with the upstream activator NIK (NF- k B-inducing kinase). In contrast to IKK a , IKK a - D LH failed to associate with either itself, IKK a , IKK b , or NEMO-IKK g -IKKAP1, while IKK a - D H complexed with IKK b and IKK a but not with NEMO. Interestingly, each IKK a isoform rescued HEK293 cells from the inhibitory effects of a dominant-negative NEMO mutant, while IKK a could not. IKK a - D Cm, a recombinant mutant of IKK a structurally akin to IKK a - D LH, was equally functional in these assays, but in sharp contrast, IKK b - D Cm, a structurally analogous mutant of IKK b , was inactive. Our results demonstrate that the functional roles of seemingly analogous domains in IKK a and IKK b need not be equivalent and can also exhibit different contextual dependencies. The existence of cytokine-inducible IKK a - D H and IKK a - D LH isoforms illustrates potential modes of NF- k B activation, which are not subject to the same in vivo regulatory constraints as either IKK a or IKK b .
Business Risk Exposure: Evidence from Informal Market Traders in Ghana

This study explores the factors influencing risk exposure among informal urban market traders in Ghana. Using the number of times of business risk exposure as the outcome variable, the study employed Poisson estimation technique to examine data on 612 respondents drawn from informal urban market traders. The study shows that regulation of business activities, market levy, number of dependents, level of formal education and numbers of economic activities engaged in are the major predictors of business risk exposure among the informal commercial market traders in Ghana. The study recommends enforcement of market regulatory compliance policy among the traders. It is also recommended that the government continue to invest in free universal basic education policy for all citizens to enhance human resource capacity and improve cognitive ability to adopt proactive measures in order to avert the occurrence of business risk exposures among the informal market traders.
Future sensitivity of neutrino telescopes to dark matter annihilations from the cosmic diffuse neutrino signal

Cosmological observations and cold dark matter N-body simulations indicate that our Universe is populated by numerous halos, where dark matter particles annihilate, potentially producing Standard Model particles. In this paper we calculate the contribution to the diffuse neutrino background from dark matter annihilations in halos at all redshifts and we estimate the future sensitivity to the annihilation cross section of neutrino telescopes such as IceCube or ANTARES. We consider various parametrizations to describe the internal halo properties and for the halo mass function in order to bracket the theoretical uncertainty in the limits from the modeling of the cosmological annihilation flux. We find that observations of the cosmic diffuse neutrino flux at large angular distances from the galactic center lead to constraints on the dark matter annihilation cross section which are complementary to (and for some extrapolations of the astrophysical parameters, better than) those stemming from observations of the Milky Way halo, especially for neutrino telescopes not pointing directly to the Milky Way center, as is the case of IceCube.
Injury Induces Genome-Wide Methylomic, Network Perturbations in Brain and Blood Predicting Neurological Disorders.

The complexity of the traumatic brain injury (TBI) pathology, particularly concussive injury, is a serious obstacle for diagnosis, treatment, and long-term prognosis. Here we utilize modern systems biology in a rodent model of concussive injury to gain a thorough view of the impact of TBI on fundamental aspects of gene regulation, which havethepotentialtodriveoralterthecourseoftheTBIpathology.TBIperturbedepigenomicprogramming,tran-scriptional activities (expression level and alternative splicing), and the organization of genes in networks cen- tered around genes such as Anax2 , Ogn , and Fmod . Transcriptomic signatures in the hippocampus are involved in neuronal signaling, metabolism, in ﬂ ammation, and blood function, and they overlap with those in leukocytes from peripheralblood.The homology between genomic signaturesfrom bloodand brain elicitedbyTBI provides proof of concept information for development of biomarkers of TBI based on composite genomic patterns. By intersecting with human genome-wide association studies, many TBI signature genes and network regulators identi ﬁ edinourrodentmodelwerecausallyassociatedwithbraindisorderswithrelevantlinktoTBI.Theoverall results show that concussive brain injury reprograms genes which could lead to predisposition to neurological and psychiatric disorders, and that genomic information from peripheral leukocytes has the potential to predict TBI pathogenesis in the brain. 2011; Derry et al., 2010; Emilsson et al., 2008) each in-volving hundreds of individuals, using a previously established method by Zhu et al. (Zhu et al., 2008, 2007). These studies are all population-186
X-ray and radio observations of flares from the RS Canum Venaticorum system UX Arietis.

Les observations RX et les observations radio de la binaire de type RS CV n , UX Ari, sont utilisees pour les mesures de l'emission quiescente et l'etude de plusieurs eruptions de l'etoile. La presence d'un plasma thermique mince tres chaud est confirmee. Les parametres du plasma des eruptions RX, la pression et l'intensite du champ magnetique ainsi que les raies d'emission du fer sont discutes
Nowcasting severe convection in the Alpine region: the COALITION approach

The purpose of the operationally oriented system named the Context and Scale Oriented Thunderstorm Satellite Predictors Development (COALITION) is automatically to detect severe thunderstorms early in their development and consequently help weather forecasters to increase lead times when issuing severe weather warnings. This new object‐oriented system integrates data provided by different sources. Data from the Meteosat Second Generation Rapid Scan Service, weather radar and numerical weather prediction, as well as climatology, are utilized by the system. One of its primary purposes is to use all the best operationally available information about convective processes and to integrate it into a heuristic model. Furthermore the orographic forcing, which is often neglected in heuristic nowcasting models, is taken into account and included in the system as an additional convective triggering mechanism. This is particularly important for areas characterized by complex orography like the Alpine region. The COALITION algorithm merges evolving thunderstorm properties with selected predictors. The forecast evolution of the storm is the result of the interaction between convective signatures and surrounding storm environment. Eight different ‘object‐environment’ interactions are analyzed in eight modules, providing ensemble nowcasts of thunderstorm attributes (satellite‐ and radar‐based) for the following 60 min. All ensemble nowcasts are then combined through a weighting and thresholding scheme and the results are summarized into a single graphical map in order to facilitate user interpretation. The COALITION nowcast system has an update frequency of 5 min. The output highlights the cells having a high probability of severe thunderstorm development within the next 30 min. Verification statistics confirm that COALITION is able to nowcast the intensity of developing convective cells with sufficient skill up to a lead time of about 20 min.
Sharp L^p estimates for Schrödinger groups

Consider a non-negative self-adjoint operator H in L2(Rd). We suppose that its heat operator e−tHe satisfies an off-diagonal algebraic decay estimate, for some exponents p0∈[0,2]. Then we prove sharp Lp→Lp frequency truncated estimates for the Schrodinger group eitH for p∈[p0,p′0]. In particular, our results apply to every operator of the form H=(i∇+A)2+V, with a magnetic potential A∈L2loc(Rd,Rd) and an electric potential V whose positive and negative parts are in the local Kato class and in the Kato class, respectively.
CQL3D-HYBRID-FOW Modeling of the Temporal Dynamics of NSTX NBI plus HHFW Discharges

The CQL3D Fokker-Planck code[1] has been upgraded to include physics of finite-orbit-width (FOW) guiding-center orbits[2,3], as compared with the previous zero-orbit-width (ZOW) model, and a recent first-order orbit calculation[2]. The Fast Ion Diagnostic FIDA[4,5] signal resulting from neutral beam (NBI) and high harmonic fast wave (HHFW) RF power injected into the NSTX spherical tokamak can now be modeled quite accurately, using ion distributions from the CQL3D-Hybrid-FOW code, a rapidly executing variant that includes FOW+gyro-orbit losses to the plasma edge, FOW effects on NBI injection and HHFW diffusion, but does not include neoclassical radial diffusion. Accurate simulation of prompt fast ion (FI) losses is a key feature of the marked modeling improvement relative to previous ZOW results. By comparing NBI-only and NBI+HHFW shots, independent confirmation of the usual 35% edge loss of HHFW in NSTX is obtained. Further, HHFW prompt losses from the plasma core are shown to be 3X as large (>25%) as the NBI-only case. The modulated NBI and time-dependent background plasma variations and charge exchange losses of fast ions are accounted for, and the temporal neutron variation is in approximate agreement with NSTX observations.