Miracle-dz/UniFAR-FTUs huggingface dataset:
High-Alpine Permafrost and Active-Layer Soil Microbiomes Differ in Their Response to Elevated Temperatures

The response of microbial communities to the predicted rising temperatures in alpine regions might be an important part of the ability of these ecosystems to deal with climate change. Soil microbial communities might be significantly affected by elevated temperatures, which influence the functioning of soils within high-alpine ecosystems. To evaluate the potential of the permafrost microbiome to adapt to short-term moderate and extreme warming, we set up an incubation experiment with permafrost and active soil layers from northern and southern slopes of a high-alpine mountain ridge on Muot da Barba Peider in the Swiss Alps. Soils were acclimated to increasing temperatures (4–40°C) for 26 days before being exposed to a heat shock treatment of 40°C for 4 days. Alpha-diversity in all soils increased slightly under gradual warming, from 4 to 25°C, but then dropped considerably at 40°C. Similarly, heat shock induced strong changes in microbial community structures and functioning in the active layer of soils from both northern and southern slope aspects. In contrast, permafrost soils showed only minor changes in their microbial community structures and no changes in their functioning, except regarding specific respiration activity. Shifts in microbial community structures with increasing temperature were significantly more pronounced for bacteria than for fungi, regardless of the soil origin, suggesting higher resistance of high-alpine fungi to short-term warming. Firmicutes, mainly represented by Tumebacillus and Alicyclobacillaceae OTUs, increased strongly at 40°C in active layer soils, reaching almost 50% of the total abundance. In contrast, Saccharibacteria decreased significantly with increasing temperature across all soil samples. Overall, our study highlights the divergent responses of fungal and bacterial communities to increased temperature. Fungi were highly resistant to increased temperatures compared to bacteria, and permafrost communities showed surprisingly low response to rising temperature. The unique responses were related to both site aspect and soil origin indicating that distinct differences within high-alpine soils may be driven by substrate limitation and legacy effects of soil temperatures at the field site.
Competence in Streptococcus pneumoniae and Close Commensal Relatives: Mechanisms and Implications

The mitis group of streptococci comprises species that are common colonizers of the naso-oral-pharyngeal tract of humans. Streptococcus pneumoniae and Streptococcus mitis are close relatives and share ~60–80% of orthologous genes, but still present striking differences in pathogenic potential toward the human host. S. mitis has long been recognized as a reservoir of antibiotic resistance genes for S. pneumoniae, as well as a source for capsule polysaccharide variation, leading to resistance and vaccine escape. Both species share the ability to become naturally competent, and in this context, competence-associated killing mechanisms such as fratricide are thought to play an important role in interspecies gene exchange. Here, we explore the general mechanism of natural genetic transformation in the two species and touch upon the fundamental clinical and evolutionary implications of sharing similar competence, fratricide mechanisms, and a large fraction of their genomic DNA.
Akt/AS160 Signaling Pathway Inhibition Impairs Infection by Decreasing Rab14-Controlled Sphingolipids Delivery to Chlamydial Inclusions

Chlamydia trachomatis, an obligate intracellular bacterium, intercepts different trafficking pathways of the host cell to acquire essential lipids for its survival and replication, particularly from the Golgi apparatus via a Rab14-mediated transport. Molecular mechanisms underlying how these bacteria manipulate intracellular transport are a matter of intense study. Here, we show that C. trachomatis utilizes Akt/AS160 signaling pathway to promote sphingolipids delivery to the chlamydial inclusion through Rab14-controlled vesicular transport. C. trachomatis provokes Akt phosphorylation along its entire developmental life cycle and recruits phosphorylated Akt (pAkt) to the inclusion membrane. As a consequence, Akt Substrate of 160 kDa (AS160), also known as TBC1D4, a GTPase Activating Protein (GAP) for Rab14, is phosphorylated and therefore inactivated. Phosphorylated AS160 (pAS160) loses its ability to promote GTP hydrolysis, favoring Rab14 binding to GTP. Akt inhibition by an allosteric isoform-specific Akt inhibitor (iAkt) prevents AS160 phosphorylation and reduces Rab14 recruitment to chlamydial inclusions. iAkt further impairs sphingolipids acquisition by C. trachomatis-inclusion and provokes lipid retention at the Golgi apparatus. Consequently, treatment with iAkt decreases chlamydial inclusion size, bacterial multiplication, and infectivity in a dose-dependent manner. Similar results were found in AS160-depleted cells. By electron microscopy, we observed that iAkt generates abnormal bacterial forms as those reported after sphingolipids deprivation or Rab14 silencing. Taken together, our findings indicate that targeting the Akt/AS160/Rab14 axis could constitute a novel strategy to limit chlamydial infections, mainly for those caused by antibiotic-resistant bacteria.
Gold-Sensitized Silicon/ZnO Core/Shell Nanowire Array for Solar Water Splitting

Solar water splitting represents one of the most promising strategies in the quest for clean and renewable energy. However, low conversion efficiency, use of sacrificial agents, and external bias for current water splitting system limit its practical application. Here, a gold-sensitized Si/ZnOcore/shell nanowire photoelectrochemical (PEC) cell is reported for efficient solar water oxidation. We demonstrated gold-sensitized n-Si/n-ZnO nanowire arrays exhibited higher energy conversion efficiency than gold-sensitized p-Si/n-ZnO nanowire arrays due to the favorable energy-band alignment characteristics. Without any assistance from an external electrical source and sacrificial reagents, gold-sensitized n-Si/n-ZnO core/shell nanowire array photoanode achieved unbiased water splitting under simulated solar light illumination. This method opens a promising venue to cost-efficient production of solar fuels.
Holocene Thermokarst Lake Dynamics in Northern Interior Alaska: The Interplay of Climate, Fire, and Subsurface Hydrology

The current state of permafrost in Alaska, and meaningful expectations for its future evolution are better informed by long-term perspectives of previous permafrost degradation. Thermokarst processes in permafrost landscapes often leads to widespread lake formation. The spatial and temporal evolution of thermokarst lake landscapes reflects the combined effects of climate, ground conditions, vegetation, and fire. To further explore the Holocene history of Yukon Flats thermokarst lakes, we conducted a detailed study of Greenpepper lake, located on the southern loess uplands, including bathymetry and sediment core analyses across a water depth transect. The sediment results, dated by radiocarbon and 210Pb, indicate that Greenpepper basin development initiated ~8000 cal yr BP through inferred thermokarst processes. Thermokarst expansion to the modern lake’s near-shore shallow water depths continued to ~5000 and 4000 cal yr BP. Subsequently stable sediment chronologies and properties indicate the lake established levels ~4 m higher than present. At that time, water overflowed into an over-deepened gully system no longer occupied by perennial streams and connected to nearby partially drained lakes. By ~1000 cal yr BP, Greenpepper lake levels had lowered below the outflow elevation, which is interpreted to reflect a response to drier conditions and possibly to downgradient catastrophic lake drainage that increased subsurface water loss, although this mechanism is untested. Timing of Greenpepper thermokarst initiation and lake expansion was later than previously documented elsewhere in the southern loess upland and post-dates the establishment of spruce forest. After the lake stabilized by ~4 ka, the late Holocene millennial trend of increased aridity corresponds with Yukon Flats regional fire and paleoclimate reconstructions and suggest linkages with large scale Holocene patterns of North Pacific atmospheric circulation.
Abnormal Phase Coupling in Parkinson’s Disease and Normalization Effects of Subthreshold Vestibular Stimulation

The human brain is a highly dynamic structure requiring dynamic coordination between different neural systems to perform numerous cognitive and behavioral tasks. Emerging perspectives on basal ganglia (BG) and thalamic functions have highlighted their role in facilitating and mediating information transmission among cortical regions. Thus, changes in BG and thalamic structures can induce aberrant modulation of cortico-cortical interactions. Recent work in deep brain stimulation (DBS) has demonstrated that externally applied electrical current to BG structures can have multiple downstream effects in large-scale brain networks. In this work, we identified EEG-based altered resting-state cortical functional connectivity in Parkinson’s disease (PD) and examined effects of dopaminergic medication and electrical vestibular stimulation (EVS), a non-invasive brain stimulation (NIBS) technique capable of stimulating the BG and thalamus through vestibular pathways. Resting EEG was collected from 16 PD subjects and 18 age-matched, healthy controls (HC) in four conditions: sham (no stimulation), EVS1 (4–8 Hz multisine), EVS2 (50–100 Hz multisine) and EVS3 (100–150 Hz multisine). The mean, variability, and entropy were extracted from time-varying phase locking value (PLV), a non-linear measure of pairwise functional connectivity, to probe abnormal cortical couplings in the PD subjects. We found the mean PLV of Cz and C3 electrodes were important for discrimination between PD and HC subjects. In addition, the PD subjects exhibited lower variability and entropy of PLV (mostly in theta and alpha bands) compared to the controls, which were correlated with their clinical characteristics. While levodopa medication was effective in normalizing the mean PLV only, all EVS stimuli normalized the mean, variability and entropy of PLV in the PD subject, with the exact extent and duration of improvement a function of stimulus type. These findings provide evidence demonstrating both low- and high-frequency EVS exert widespread influences on cortico-cortical connectivity, likely via subcortical activation. The improvement observed in PD in a stimulus-dependent manner suggests that EVS with optimized parameters may provide a new non-invasive means for neuromodulation of functional brain networks.
The Kaali Impact as Trigger of a Mega-Tsunami Event and Violent Seismotectonics in Sweden

A meteorite impacted the ground in Estonia forming the Kaali Crater dated at 1183 - 1162 BC. It set up a Baltic-wide mega-tsunami dated at 1171 absolute varve years BC. It also triggered violent seismotectonic effect in Sweden. Ancient legends testify of direct observations of the sky phenomena and personal experiences of the ground shaking and tsunami flooding, which makes the Kaali Crater the oldest impact event documented by humans. The Ragnarok apocalypse is likely to lead its origin from the violent geodynamic activity along the Swedish east coast.
The effect of corporate governance and firm-specific characteristics on the incidence of financial restatement

Purpose This paper aims to examine the effects of corporate governance and firm-specific characteristics on the incidence of financial restatement among Malaysian public listed firms. Design/methodology/approach The elements of corporate governance consist of board size, board independence, multiple directorships, audit committee expertise, external audit quality and executive compensation. Meanwhile, the firm-specific characteristics consist of firm age, firm performance, firm leverage and firm liquidity. The agency theory has been used to guide the study. This study used a matched-pair sample that consisted of a sample of 49 restatement firms and 98 non-restatement firms between the years 2011 and 2016. Univariate (t-test and Pearson correlation) and multivariate (logistic regression) statistical techniques were used to test the hypotheses. Findings The results show that there is a negative and significant relationship between executive compensation and firm performance, and the incidence of financial restatement. In addition, there is a positive and significant relationship between firm leverage and the incidence of financial restatement. However, the other corporate governance and firm-specific characteristic variables included in the study were found to be insignificant with the incidence of financial restatement. This paper provides evidence that some form of corporate governance mechanisms and firm-specific characteristics, particularly executive compensation, firm performance and firm leverage, may influence the direction and magnitude of the incidence of financial restatement. The findings indicate that optimal executive incentives may align management interests with those of shareholders. In addition, greater performance and lower leverage levels minimise firms’ financial pressure and debt covenant violation risk, which may reduce the management tendency to misstate the financial statement, and consequently, minimise the likelihood of financial restatement. Originality/value The main value of this paper is the effect of corporate governance and firm-specific characteristics on the likelihood of financial restatement in Malaysia. The findings of this study provide useful insights for regulators to improve and reconsider the current regulations on corporate governance mechanisms.
Developing a Next-of-Kin Involvement Guide in Cancer Care—Results From a Consensus Process

Background In hospital cancer care, there is no set standard for next-of-kin involvement in improving the quality of care and patient safety. There is therefore a growing need for tools and methods that can guide this complex area. Objective The aim of this study was to present the results from a consensus-based participatory process of designing a guide for next-of-kin involvement in hospital cancer care. Method A consensus process based on a modified Nominal group technique was applied with 20 stakeholder participants from 2 Norwegian university hospitals. Result The participants agreed on the 5 most important priorities for hospital cancer care services when involving next-of-kin. The results showed that next-of-kin stakeholders, when proactively involved, are important resources for the patient and healthcare professionals in terms of contribution to quality and safety in hospitals. Suggested means of involving next-of-kin were closer interaction with external support bodies, integration in clinical pathways, adjusted information, and training healthcare professionals. Conclusion In this study, we identified topics and elements to include in a next-of-kin involvement guide to support quality and safety in hospital cancer care. The study raises awareness of the complex area of next-of-kin involvement and contributes with theory development and knowledge translation in an involvement guide tailored for use by healthcare professionals and managers in everyday clinical practice. Implications for Practice Service providers can use the guide to formulate intentions and make decisions with suggestions and priorities or as a reflexive tool for organizational improvement.
Variable selection for longitudinal varying coefficient errors-in-variables models

Abstract In this paper, we investigate the variable selection for varying coefficient errors-in-variables (EV) models with longitudinal data when some covariates are measured with additive errors. A variable selection method based on bias-corrected penalized quadratic inference function (pQIF) is proposed by combining the basis function approximation to coefficient functions and bias-corrected quadratic inference function (QIF) with shrinkage estimations. The proposed method can handle the measurement errors of covariates and within-subject correlation, estimate and select non-zero nonparametric coefficient functions. With appropriate selection of the tuning parameters, we establish the consistency of the variable selection method and the sparsity properties of the regularized estimators. The finite sample performance of the proposed method is assessed by simulation studies. The utility of the method is further demonstrated via a real data analysis.
A Systematic Review of Remotely Operated Vehicle Surveys for Visually Assessing Fish Assemblages

Anthropogenic activities and greater demands for marine natural resources has led to increases in the spatial extent and duration of pressures on marine ecosystems. Remotely operated vehicles (ROVs) offer a robust survey tool for quantifying these pressures and tracking the success of management intervention while at a range of depths, including those inaccessible to most SCUBA diver-based survey methods (∼>30 m). As the strengths, limitations, and biases of ROVs for visually monitoring fish assemblages remain unclear, this review aims to evaluate ROVs as a survey technique and to suggest optimal sampling strategies for use in typical ROV-based studies. Using the search engines Scopus™ and Google Scholar™, 119 publications were identified that used ROVs for visual surveys of fish assemblages. While the sampling strategies and sampling metrics used to annotate the imagery in these publications varied considerably, the total abundance of fish recorded over strip transects of varying dimensions was the most common sampling design. The choice of ROV system appears to be a strong indicator of both the types of surveys available to studies and the success of ROV deployments. For instance, larger, more powerful working-class systems can complete longer and more complex designs (e.g., swath, cloverleaf, and polygonal transects) at greater depths, whereas observation-class systems are less expensive and easier to deploy, but are more susceptible to delays or cancellations of deployments. In more severe sea state conditions, radial transects, or strip transects that employ live-boating or a weight to anchor the tether to the seafloor, can be used to improve the performance of observation-class systems. As these systems often employ shorter tethers, radial transects can also be used to maximize sampling area at greater depths and on large vessels that may rotate substantially while anchored. For highly mobile species, and in survey designs where individuals are likely to be recounted (e.g., transects along oil and gas pipelines), relative abundance (MaxN) may be a more robust sampling metric. By identifying subtle, yet important, differences in the application of ROVs as a tool for visually surveying deep-water marine ecosystems, we identified key areas for improvement for best practice for future studies.
Female Speakers Benefit More Than Male Speakers From Prosodic Charisma Training—A Before-After Analysis of 12-Weeks and 4-h Courses

Empirical studies not just show that perceived charisma is an important success factor in professional life; they also show that female speakers need to overcome higher intrinsic and extrinsic hurdles than male speakers in order to create the same charismatic effect. Prosody is probably the most influential instrument for speaker charisma. We have developed a system called "Pascal" that analyzes and assesses on objective acoustic grounds the use of seven prosodic parameters in business presentations. It is used for charismatic-speech training in 12-week and 4-hour courses on entrepreneurship and leadership. Comparing the Pascal scores for a total of 72 participants at the beginning and end of these two course types showed that female speakers start with significantly lower prosodic-charisma scores, but then learn so much faster and more than male speakers that they are able to catch up with male speakers in 4-hour courses and significantly reduce the gap to male speakers in 12-week courses. Given that leadership and entrepreneurship are still male-dominated domains, our results can be seen as an encouragement for women to attend prosodic charisma trainings.
NCS-1 Deficiency Is Associated With Obesity and Diabetes Type 2 in Mice

Neuronal calcium sensor-1 (NCS-1) knockout (KO) in mice (NCS-1−/− mice) evokes behavioral phenotypes ranging from learning deficits to avolition and depressive-like behaviors. Here, we showed that with the onset of adulthood NCS-1−/− mice gain considerable weight. Adult NCS-1−/− mice are obese, especially when fed a high-fat diet (HFD), are hyperglycemic and hyperinsulinemic and thus develop a diabetes type 2 phenotype. In comparison to wild type (WT) NCS-1−/− mice display a significant increase in adipose tissue mass. NCS-1−/− adipocytes produce insufficient serum concentrations of resistin and adiponectin. In contrast to WT littermates, adipocytes of NCS-1−/− mice are incapable of up-regulating insulin receptor (IR) concentration in response to HFD. Thus, HFD-fed NCS-1−/− mice exhibit in comparison to WT littermates a significantly reduced IR expression, which may explain the pronounced insulin resistance observed especially with HFD-fed NCS-1−/− mice. We observed a direct correlation between NCS-1 and IR concentrations in the adipocyte membrane and that NCS-1 can be co-immunoprecipitated with IR indicating a direct interplay between NCS-1 and IR. We propose that NCS-1 plays an important role in adipocyte function and that NCS-1 deficiency gives rise to obesity and diabetes type 2 in adult mice. Given the association of altered NCS-1 expression with behaviorial abnormalities, NCS-1−/− mice may offer an interesting perspective for studying in a mouse model a potential genetic link between some psychiatric disorders and the risk of being obese.