Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of risk factors for pulmonary tuberculosis with persistent severe inflammation

Abstract Introduction: Patients with pulmonary tuberculosis (TB) sometimes show persistent severe inflammation for more than 1 month, even if TB treatment is effective. Although this inflammation can be improved through continuous antituberculous therapy, the risk factors for persistent inflammation remain unclear. Therefore, we sought to study the characteristics of patients with persistent severe inflammation. Materials and methods: We retrospectively analyzed 147 hospitalized adult patients with C-reactive protein (CRP) levels of 5 mg/dL or more on admission to Fukujuji Hospital from April 2019 to March 2021. The patients were divided into 2 groups: 40 patients (27.2%) had CRP levels of 5 mg/dL or more at 4 weeks after admission (persistent inflammation group), and 107 patients (72.8%) had CRP levels that fell below 5 mg/dL within 4 weeks of admission (improved inflammation group). Results: The median CRP level on admission in the persistent inflammation group was 10.8 mg/dL (interquartile range 9.1–14.5), which was higher than that in the improved inflammation group (median 8.2 mg/dL [6.5–12.1], P = .002). Patients in the persistent inflammation group had a higher prevalence of large cavities, defined as cavities ≥4 cm in diameter, on chest computed tomography (CT) (n = 20 [50.0%] vs n = 12 [11.2%], P < .001). Discussion and conclusions: This study showed that 27.2% of patients who had high or moderate inflammation on admission did not achieve low CRP levels within 4 weeks after admission. Risk factors for persistent severe inflammation in patients with TB were presence of a large cavity (cavity diameter ≥4 cm) on chest CT and a high CRP level on admission. Therefore, in a patient with a large cavity on chest CT and/or CRP ≥9.0 mg/dL on admission, long-term inflammation may occur despite antituberculous therapy if other diseases are ruled out.
Transplantation of tissue-engineered human corneal endothelium in cat models

Purpose To evaluate the performance of reconstructed tissue-engineered human corneal endothelium (TE-HCE) by corneal transplantation in cat models. Methods TE-HCE reconstruction was performed by culturing 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-labeled monoclonal HCE cells on denuded amniotic membranes (dAMs) in 20% fetal bovine serum-containing Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 (1:1) medium and 5% CO2 at 37 °C on a 24-well culture plate. The reconstructed TE-HCE was transplanted into cat corneas via lamellar keratoplasty with all of the endothelium and part of Descemet’s membrane stripped. Postsurgical corneas were monitored daily with their histological properties examined during a period of 104 days after transplantation. Results The reconstructed TE-HCE at a density of 3,413.33±111.23 cells/mm2 in average established intense cell-cell and cell-dAM junctions. After lamellar keratoplasty surgery, no obvious edema was found in TE-HCE-transplanted cat corneas, which were transparent throughout the monitoring period. In contrast, intense corneal edema developed in dAM-transplanted cat corneas, which were turbid. The corneal thickness gradually decreased to 751.33±11.37 μm on day 104 after TE-HCE transplantation, while that of dAM eye was over 1,000 μm in thickness during the monitoring period. A monolayer of endothelium consisting of TE-HCE-originated cells at a density of 2,573.33±0.59 cells/mm2 attached tightly to the surface of remnant Descemet’s membrane over 104 days; this was similar to the normal eye control in cell density. Conclusions The reconstructed TE-HCE was able to function as a corneal endothelium equivalent and restore corneal function in cat models.
FGFR1 Induces Acquired Resistance Against Ge ﬁ tinib By Activating AKT/mTOR Pathway In NSCLC

Objective: As an epidermal growth factor, receptor-tyrosine kinase inhibitor (EGFR-TKI), ge ﬁ tinib demonstrates a good therapeutic effect in patients with EGFR-mutant non-small-cell lung cancer (NSCLC). However, an overwhelming majority of these patients inevitably develop resistance against ge ﬁ tinib. Unfortunately, the mechanism underlying this phenom-enon is still not fully understood. Here we aim to reveal the mechanism of ge ﬁ tinib resistance in NSCLC induced by FGFR1. Materials and methods: We used high-throughput sequencing to compare the mRNA expression pro ﬁ les of PC9 and PC9-GR (ge ﬁ tinib-resistant) cells. The clinical signi ﬁ cance of ﬁ broblast growth factor receptor 1 (FGFR1) in NSCLC was also investigated using immunohistochemistry and Kaplan-Meier survival analysis. Finally, the in vitro molecular mechanisms were analyzed using confocal laser microscopy, Western blotting, transwell assay, colony formation assay, CCK-8 assay, and apoptosis assay. Results: We observed that FGFR1 was highly expressed in NSCLC tissues and was closely associated with poor prognosis. Cytological experiments showed that FGFR1 promoted the proliferation and migration of PC9-GR cells and mediated their resistance to ge ﬁ tinib. Furthermore, studies aimed at unraveling this mechanism revealed that FGFR1 activated the AKT/mTOR signaling pathway. These ﬁ ndings show that the FGFR1/AKT/mTOR signaling pathway plays a vital role in acquired resistance against ge ﬁ tinib in NSCLC. Conclusion: This work provides new evidence that FGFR1 functions as a key regulator of ge ﬁ tinib resistance, thereby demonstrating its potential as a novel biomarker and therapeutic target for NSCLC.
health-related quality of life in current smokers with COPD: factors associated with current smoking and new insights into sex differences

: Findings from studies that examined the association between health-related quality of life (HRQOL) and smoking status among COPD patients have been mixed. Moreover, factors associated with current smoking in COPD patients and differences by sex have not been fully elucidated. Data from the 2011 and 2012 Behavioral Risk Factor Surveillance System was used in this study. Four HRQOL indicators were examined in this study: general health, physical health, mental health, and activity limitations. General health was dichotomized into two groups: “excellent/very good/good” and “fair/poor”, and the other three HRQOL indicators were dichotomized into , 14 (infrequent) and $ 14 (frequent) unhealthy days in the past 30 days. To examine HRQOL indicators in association with current versus former smoking and identify factors associated with current smoking, logistic regression models were used. Sex differences were explored. In COPD patients, current smokers compared to former smokers had significantly poor HRQOL on all subdomains: “fair/ poor” general health (adjusted odds ratio [AOR]: 1.2 [95% confidence interval {CI}: 1.1–1.5]); poor physical health (AOR: 1.3 [CI: 1.1–1.5]); poor mental health (AOR: 1.8 [CI: 1.4–2.2]); and poor activity limitations (AOR: 1.5 [CI: 1.3–1.9]). HRQOL subdomains affected by current smoking differed by sex except activity limitations. General health (AOR: 1.5 [CI: 1.1–2.0]) and activity limitations (AOR: 1.6 [95% CI: 1.2–2.2]) in males and physical health (AOR: 1.3 [CI: 1.0–1.6]), mental health (AOR: 2.1 [CI: 1.7–2.6]), and activity limitations (AOR: 1.5 [CI: 1.2–1.9]) in females were significantly impaired due to current smoking. Factors associated with current smoking differed by sex except being unmarried and having less than a college degree, which were associated with current smoking in both males and females. These findings have important implications for health providers in designing more effective interventions which tailor to and target specific subgroups for smoking cessation.
Comparative study of two different respiratory training protocols in elderly patients with chronic obstructive pulmonary disease

Aim: The aim of the present study was to compare threshold inspiratory muscle training (IMT) and expiratory muscle training (EMT) in elderly male patients with moderate degree of COPD. Materials and methods: Forty male patients with moderate degree of COPD were recruited for this study. They were randomly divided into two groups: the IMT group who received inspiratory training with an intensity ranging from 15% to 60% of their maximal inspiratory pressure, and the EMT group who received expiratory training with an equal intensity which was adjusted according to the maximal expiratory pressure. Both groups received training three times per week for 2 months, in addition to their prescribed medications. Results: Both IMT and EMT groups showed a significant improvement in forced vital capacity, forced expiratory volume in the first second, forced expiratory volume in the first second% from the predicted values, and forced vital capacity% from the predicted value, with no difference between the groups. Both types of training resulted in a significant improvement in blood gases (SaO 2 %, PaO 2 , PaCO 2 , and HCO 3 ), with the inspiratory muscle group showing the best results. Both groups showed a significant improvement in the 6-min walking distance: an increase of about 25% in the inspiratory muscle group and about 2.5% in the expiratory muscle group. Conclusion: Both IMT and EMT must be implemented in pulmonary rehabilitation programs in order to achieve improvements in pulmonary function test, respiratory muscle strength, blood oxygenation, and 6-min walking distance.
Tectonics of the Strandja Massif , NW Turkey : History of a Long-Lived Arc at the Northern Margin of Palaeo-Tethys

Th e Strandja Massif, Th race Peninsula, NW Turkey, forms an important link between the Balkan Zone of Bulgaria, which is usually correlated with Variscan orogen in Central Europe, and the Pontides, where Cimmerian structures are the most prominent. Th e massif is composed of a Palaeozoic basement and a Triassic metasedimentary cover. Th e basement is made of various granite gneisses, paragneisses, and schists that are intruded by large plutons of monzonitic metagranites. Detrital zircon studies have revealed Ordovician (433 and 446 Ma) and Carboniferous (305 Ma) ages of the metasedimentary rocks. Th e isotopic age of the granite gneisses is 308–315 Ma (Carboniferous, Bashkirian–Moscovian) as single zircon evaporation method and conventional U-Pb technique show. Th e Palaeozoic basement was deformed and metamorphosed before the emplacement of the large monzonitic metagranite plutons yielding zircon ages from 309±24 to 257 Ma (Moscovian–Permian). Geochemical features of the Carboniferous and Permian magmatic rocks indicate a subduction-related tectonic setting similar to coeval rocks exposed in the Balkan zone of Bulgaria. Th e Triassic metasedimentary cover unconformably overlies the basement with basal conglomerate and arkosic sandstone that pass upward into a thick pile of lithic metasandstones and a metasandstone/pelitic schist alternation. Calcareous metasandstones and black slates appear at the highest structural levels. Th e Triassic succession reveals obvious orogenic features judged from its great thickness, sedimentary features indicating high-energy currents and the presence of intermediate pillow lavas. Both the basement and the cover units were aff ected by strong deformation and epidote-amphibolite to greenschist facies metamorphism during the Late Jurassic–Early Cretaceous. Th is event was terminated by the emplacement of a nappe of unmetamorphosed Jurassic limestones and dolomites occurring at the top of the structural column. Kinematic indicators in mylonites at the base of the nappe suggest its original location in the south. Th e Strandja Massif shows remarkable similarity to the late Palaeozoic–early Mesozoic Silk Road arc that evolved at the southern margin of Eurasia due to the northward subduction of Palaeo-Tethys (Natal'in & Şengör 2005). Th e fragments of this arc are exposed in Caucasus, Iran, South Tien Shan, Pamir, and Kunlun. Th e Precambrian history of the Strandja Massif, as recorded by detrital and inherited zircon ages, reveals many common features with the Baltica-Timanide collage including its fragments distributed in Central Asia. Various sets of data and correlations with surrounding tectonic units show that the Strandja Massif is a fragment of the long-lived, Ordovician to Triassic Silk Road magmatic arc, which evolved on the northern side of Palaeo-Tethys.
Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus

Probiotic microbes rely on their ability to survive in the gastrointestinal tract, adhere to mucosal surfaces, and metabolize available energy sources from dietary compounds, including prebiotics. Genome sequencing projects have proposed models for understanding prebiotic catabolism, but mechanisms remain to be elucidated for many prebiotic substrates. Although β-galactooligosaccharides (GOS) are documented prebiotic compounds, little is known about their utilization by lactobacilli. This study aimed to identify genetic loci in Lactobacillus acidophilus NCFM responsible for the transport and catabolism of GOS. Whole-genome oligonucleotide microarrays were used to survey the differential global transcriptome during logarithmic growth of L. acidophilus NCFM using GOS or glucose as a sole source of carbohydrate. Within the 16.6-kbp gal-lac gene cluster, lacS, a galactoside-pentose-hexuronide permease-encoding gene, was up-regulated 5.1-fold in the presence of GOS. In addition, two β-galactosidases, LacA and LacLM, and enzymes in the Leloir pathway were also encoded by genes within this locus and up-regulated by GOS stimulation. Generation of a lacS-deficient mutant enabled phenotypic confirmation of the functional LacS permease not only for the utilization of lactose and GOS but also lactitol, suggesting a prominent role of LacS in the metabolism of a broad range of prebiotic β-galactosides, known to selectively modulate the beneficial gut microbiota.
Increasing water productivity in Agriculture

Increasing water productivity is an important element in improved water management for sustainable agriculture, food security and healthy ecosystem functioning. Water productivity is defined as the amount of agricultural output per unit of water depleted, and can be assessed for crops, trees, livestock and fish. This chapter reviews challenges in and opportunities for improving water productivity in socially equitable and sustainable ways by thinking beyond technologies, and fostering enabling institutions and policies. Both in irrigated and rainfed cropping systems, water productivity can be improved by choosing well-adapted crop types, reducing unproductive water losses and maintaining healthy, vigorously growing crops through optimized water, nutrient and agronomic management. Livestock water productivity can be increased through improved feed management and animal husbandry, reduced animal mortality, appropriate livestock watering and sustainable grazing management. In agroforestry systems, the key to success is choosing the right combination of trees and crops to exploit spatial and temporal complementarities in resource use. In aquaculture systems, most water is depleted indirectly for feed production, via seepage and evaporation from water bodies, and through polluted water discharge, and efforts to improve water productivity should be directed at minimizing those losses. Identifying the most promising options is complex and has to take into account environmental, financial, social and health-related considerations. In general, improving agricultural water productivity, thus freeing up water for ecosystem functions, can be achieved by creating synergies across scales and between various agricultural sectors and the environment, and by enabling multiple uses of water and equitable access to water resources for different groups in society.
Interaction of CagA with Crk plays an important role in Helicobacter pylori–induced loss of gastric epithelial cell adhesion

CagA protein is a major virulence factor of Helicobacter pylori, which is delivered into gastric epithelial cells and elicits growth factor–like responses. Once within the cells, CagA is tyrosine phosphorylated by Src family kinases and targets host proteins required to induce the cell responses. We show that the phosphorylated CagA binds Crk adaptor proteins (Crk-II, Crk-I, and Crk-L) and that the interaction is important for the CagA-mediated host responses during H. pylori infection. H. pylori–induced scattering of gastric epithelial cells in culture was blocked by overexpression of dominant-negative Crk and by RNA interference–mediated knockdown of endogenous Crk. H. pylori infection of the gastric epithelium induced disruption of E-cadherin/catenin–containing adherens junctions, which was also dependent on CagA/Crk signaling. Furthermore, inhibition of the SoS1/H-Ras/Raf1, C3G/Rap1/B-Raf, or Dock180/Rac1/Wiskott-Aldrich syndrome protein family verprolin homologous protein pathway, all of which are involved downstream of Crk adaptors, greatly diminished the CagA-associated host responses. Thus, CagA targeting of Crk plays a central role in inducing the pleiotropic cell responses to H. pylori infection that cause several gastric diseases, including gastric cancer.
Continuous versus Intermittent Infusion of Oxacillin for Treatment of Infective Endocarditis Caused by Methicillin-Susceptible Staphylococcus aureus

ABSTRACT Infective endocarditis (IE) is the fourth leading cause of life-threatening infection in the United States and imposes significant morbidity and mortality. The American Heart Association guidelines for the diagnosis and treatment of IE do not address continuous-infusion (CI) oxacillin. This retrospective study compares outcomes between CI oxacillin and intermittent-infusion (II) oxacillin in the treatment of IE caused by methicillin-susceptible Staphylococcus aureus (MSSA). A total of 709 medical records were reviewed for inpatients with definitive IE treated between 1 January 2000 and 31 December 2007. Continuous data were analyzed by Student's t test or the Wilcoxon rank sum test. The chi-square test or Fisher's exact test was used to compare nominal data. A multivariate logistic model was constructed. One hundred seven patients met eligibility criteria for inclusion into the study. Seventy-eight patients received CI oxacillin, whereas 28 received II oxacillin. CI and II groups were similar with respect to 30-day mortality (8% versus 10%, P = 0.7) and length of stay (20 versus 25 days, P = 0.4) but differed in 30-day microbiological cure (94% versus 79%, P = 0.03). Sixty-three patients received synergistic gentamicin, whereas 44 did not. The gentamicin and no-gentamicin groups were similar with respect to 30-day mortality (11% versus 4%, P = 0.2) and 30-day microbiological cure (90% versus 89%, P = 0.8); however, times to defervescence (4 versus 2 days, P = 0.02) were significantly different. CI oxacillin is an effective alternative to II oxacillin for the treatment of IE caused by MSSA and may improve microbiological cure. This convenient and pharmacodynamically optimized dosing regimen for oxacillin deserves consideration for patients with IE caused by MSSA.
Vitrectomy in patients 85 years of age and older: surgical outcomes and visual prognosis

Purpose: To evaluate visual and surgical outcomes in very elderly patients (above 85 years of age) undergoing pars plana vitrectomy (PPV). Patients and methods: A single-center, retrospective study was carried out on the medical records of 82 patients aged 85 years and older who had undergone PPV from 2006 to 2013. Patients ranged in age from 86 to 99 years, with a mean age of 88.9 years ( ± 2.88). Visual results and intraoperative and postoperative complications were the main outcome measures. Visual improvement/worsening was defined as at least ± 0.1 logMAR change. Results: Mean follow-up was 7.25 months ( ± 5.35), with a range of 1–28 months. General anesthesia was used in 63% of the operations. The most common indication was retinal detachment (27%). The ocular condition necessitating PPV was secondary to trauma (most commonly after a fall) in 10 eyes (12%). Mean visual acuity (VA) improved from 1/58 preoperatively to 1/29 at the final evaluation ( p = 0.014). Mean improvement in VA in eyes of patients with the comorbidity of age-related macular degeneration (n = 34) was 41% lower compared to eyes of patients without the disease (n = 48, p = 0.013). In the subgroup of patients operated on for retinal detachment, 45.4% did not reach primary anatomic success and 45.4% needed additional retina-affecting surgery. One or more major ocular complications were reported in 24 eyes (29%), while 19 eyes (23%) had minor ocular complications. Conclusion: Improved VA was documented in more than half of the older adults aged 85–99 undergoing vitrectomy. Despite the rate of complications in the very elderly, the possibility of optimizing visual function may positively affect quality of life in this subgroup.
Title : Ketamine corrects a deficit in reversal learning caused by chronic intermittent cold stress in female rats

© The Author(s) 2018. Published by Oxford University Press on behalf of CINP. This is an Open Access article distributed under the terms of the Creative Commons Attribution NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Title: Ketamine corrects a deficit in reversal learning caused by chronic intermittent cold stress
Application of high-precision two-way ranging to Galileo Earth-1 encounter navigation

The application of precision two-way ranging to orbit determination with relatively short data arcs is investigated for the Galileo spacecraft's approach to its first Earth encounter (December 8, 1990). Analysis of previous S-band (2.3-GHz) ranging data acquired from Galileo indicated that under good signal conditions submeter precision and 10-m ranging accuracy were achieved. It is shown that ranging data of sufficient accuracy, when acquired from multiple stations, can sense the geocentric angular position of a distant spacecraft. A range data filtering technique, in which explicit modeling of range measurement bias parameters for each station pass is utilized, is shown to largely remove the systematic ground system calibration errors and transmission media effects from the Galileo range measurements, which would otherwise corrupt the angle-finding capabilities of the data. The accuracy of the Galileo orbit solutions obtained with S-band Doppler and precision ranging were found to be consistent with simple theoretical calculations, which predicted that angular accuracies of 0.26-0.34 microrad were achievable. In addition, the navigation accuracy achieved with precision ranging was marginally better than that obtained using delta-differenced one-way range (delta DOR), the principal data type that was previously used to obtain spacecraft angular position measurements operationally.