Miracle-dz/UniFAR-FTUs huggingface dataset:
Delusion progression process from the perspective of patients with psychoses: A descriptive study based on the primary delusion concept of Karl Jaspers

Background Delusion occupies an important position in the diagnosis and treatment of patients with psychoses. Although Karl Jaspers’ concept of the primary delusion (PD) is a key hypothesis in descriptive phenomenology concerning the primordial experience of delusion, to our knowledge it has not been verified in empirical studies of patients with psychosis, and the relationship between PDs and fully developed delusions remains unclear. Methods The subjects were 108 psychiatric patients diagnosed with DSM-IV schizophrenia or schizoaffective disorder who had persisting delusions. This investigation used a newly devised semi-structured interview, the Delusion and its Origin Assessment Interview (DOAI), and the Positive and Negative Syndrome Scale. PDs enquired about in the DOAI were delusional perception, delusional memory, delusional mood, and delusional intuition. Associations of PDs with delusion themes and delusion features extracted from DOAI items by factor analysis were examined using correlational and MANCOVA regression analyses. Reliability studies of the DOAI were also conducted. Results The reliability and correlation analyses suggested robust psychometric properties of the DOAI. The percentages of subjects reporting PD phenomena as delusion origins and currently present were 93% and 84%, respectively. MANCOVA revealed several significant associations, including between delusional perception and delusional mood and persecutory themes, between delusional intuition and grandiose delusions, and between delusional perception and intuition and systematization of delusions. Discussion This study demonstrates that PDs can be considered as principal origins of delusions by subjects with psychosis, and have meaningful connections with the characteristics of their fully developed delusions. The associations between PDs and delusion characteristics can be interpreted in terms of progression processes of delusions, which are seen as intensification and generalization of cognitive and affective pathologies in PDs. The findings are also consistent with the neurobiological hypothesis that aberrant salience attribution to stimuli, as in PDs, is the primary phenomenon caused by abnormal dopamine system regulation. Further studies are needed to clarify delusion progression processes relating to PDs and to substantiate their clinical meanings.
Mothers’ neural response to valenced infant interactions predicts postpartum depression and anxiety

It is currently unknown whether differences in neural responsiveness to infant cues observed in postpartum affective disturbance are specific to depression/anxiety or are better attributed to a common component of internalizing distress. It is also unknown whether differences in mothers’ brain response can be accounted for by effects of past episodes, or if current neural processing of her child may serve as a risk factor for development of future symptoms. Twenty-four mothers from a community-based sample participated in an fMRI session viewing their 3-month- old infant during tasks evoking positive or negative emotion. They were tracked across the ensuing 15 months to monitor changes in affective symptoms. Past and current episodes of depression and anxiety, as well as future symptoms, were used to predict differences in mothers’ hemodynamic response to their infant in positive compared to negative emotion contexts. Lower relative activation in largely overlapping brain regions involving frontal lobe structures to own infant positive vs. negative emotion was associated with concurrent (3-month) depression diagnosis and prospective (3–18 month) depression and anxiety symptoms. There was little evidence for impacts of past psychopathology (more limited effect of past anxiety and nonsignificant effect of past depression). Results suggest biased maternal processing of infant emotions during postpartum depression and anxiety is largely accounted for by a shared source of variance (internalizing distress). Furthermore, differential maternal responsiveness to her infant’s emotional cues is specifically associated with the perpetuation of postpartum symptoms, as opposed to more general phenotypic or scarring effects of past psychopathology.
Object-Level Scene Context Prediction

Contextual information plays an important role in solving various image and scene understanding tasks. Prior works have focused on the extraction of contextual information from an image and use it to infer the properties of some object(s) in the image or understand the scene behind the image, e.g., context-based object detection, recognition and semantic segmentation. In this paper, we consider an inverse problem, i.e., how to hallucinate the missing contextual information from the properties of standalone objects. We refer to it as object-level scene context prediction. This problem is difficult, as it requires extensive knowledge of the complex and diverse relationships among objects in the scene. We propose a deep neural network, which takes as input the properties (i.e., category, shape, and position) of a few standalone objects to predict an object-level scene layout that compactly encodes the semantics and structure of the scene context where the given objects are. Quantitative experiments and user studies demonstrate that our model can generate more plausible scene contexts than the baselines. Our model also enables the synthesis of realistic scene images from partial scene layouts. Finally, we validate that our model internally learns useful features for scene recognition and fake scene detection.
Anti-listerial properties of chemical constituents of Eruca sativa (rocket salad): From industrial observation to in vitro activity

The frequency of foodborne outbreaks epidemiologically associated with Listeria monocytogenes in fresh produce has increased in recent years. Although L. monocytogenes may be transferred from the environment to vegetables during farming, contamination of food products most commonly occurs in food processing facilities, where L. monocytogenes has the ability to establish and persist on processing equipment. The current study was undertaken to collect data on the occurrence of L. monocytogenes and the identity of the endogenous microbiota in a fresh produce processing facility, for which information has remained scarce. L. monocytogenes was not detected in the facility. Experiments simulating conditions in the processing environment were performed, including examination of bacterial growth in nutrients based on vegetables (salad juice) compared to in other types of nutrients (fish, meat). Results showed that the endogenous microbiota (dominated by Pseudomonas) grew well in iceberg lettuce and rocket salad juice at low temperatures, while growth inhibition of L. monocytogenes was observed, particularly in rocket salad juice. The anti-listerial activity in rocket salad juice was retained in a polar chromatographic fraction containing several metabolites. Characterization of this active fraction, using LC-MS/MS, led to identification of 19 compounds including nucleosides and amino acids. Further work is necessary to determine the molecular mechanism responsible for the inhibitory activity of rocket salad constituents. The study nevertheless suggests that the available nutrients, as well as a low temperature (3 °C) and the in-house bacterial flora, may influence the prevalence of L. monocytogenes in fresh produce processing facilities.
Tumor-Associated Neutrophils Drive B-cell Recruitment and Their Differentiation to Plasma Cells

Tumor-associated neutrophils (TAN) are shown to regulate TNFα-mediated B-cell migration into the TME and their differentiation into functional plasma cells, partially mediated by interaction of TAN BAFF and B-cell BAFF-R. The data highlight potential targets for cancer therapy. A major mechanism through which neutrophils have been suggested to modulate tumor progression involves the interaction and subsequent modulation of other infiltrating immune cells. B cells have been found to infiltrate various cancer types and play a role in tumor immunity, offering new immunotherapy opportunities. Nevertheless, the specific impact of tumor-associated neutrophils (TAN) on B cells has largely been overlooked. In the current study, we aimed to characterize the role of TANs in the recruitment and modulation of B cells in the tumor microenvironment (TME). We showed that TANs actively participate in the recruitment of B cells to the TME and identified TNFα as the major cytokine mediating B-cell chemotaxis by TANs. The recruitment of CD45+B220+CD138− splenic B cells by TANs in vitro resulted in B-cell phenotypic modulation, with 68.6% ± 2.1% of the total migrated B cells displaying a CD45−B220+CD138+ phenotype, which is typical for plasma cells. This phenotype mirrored the large proportion (54.0% ± 6.1%) of CD45−B220+CD138+ intratumoral B cells (i.e., plasma cells) in Lewis lung carcinoma tumors. We next confirmed that the differentiation of CD45+B220+CD138− B cells to functionally active CD45−B220+CD138+ plasma cells required contact with TANs, was independent of T cells, and resulted in IgG production. We further identified membranal B-cell activating factor (BAFF) on TANs as a potential contact mechanism mediating B-cell differentiation, as blocking BAFF-receptor (BAFF-R) significantly reduced IgG production by 20%. Our study, therefore, demonstrates that TANs drive the recruitment and modulation of B cells into plasma cells in the TME, hence opening new avenues in the targeting of the immune system in cancer.
Narrative comprehension skills of Indigenous and non-Indigenous Australian children in their first year of school

Abstract Purpose: The suitability of existing speech-language pathology assessments for Australian Aboriginal and Torres Strait Islander (Indigenous) children is questioned in the literature. There is emerging evidence that the differences reported between Indigenous and non-Indigenous Australian children on standardised assessment are diminished on more naturalistic assessments such as narrative production (macrostructure and microstructure). Little is documented, however, about the narrative comprehension skills of Australian children. This study explores the narrative comprehension skills of 40 typically developing Indigenous and non-Indigenous Australian children in their first year of school. Method: A cross-sectional comparative research design was used. Three non-standardised narrative assessments incorporating comprehension-production protocols were administered. Question responses were scored for accuracy and categorised according to story grammar targeted and inference (literal vs. non-literal). In addition, all participants completed the Peabody Picture Vocabulary Test fourth edition (PPVT-4). Result: A repeated-measures ANOVA was used to compare response accuracy to comprehension questions between cultural groups and across narrative protocols. While there was a significant difference in PPVT-4 scores, no significant differences were identified between response accuracy for the Indigenous and non-Indigenous children. Furthermore, response accuracy to comprehension questions was correlated with PPVT-4 scores for the non-Indigenous children only. Conclusion: Findings support the use of naturalistic assessment strategies such as narrative comprehension with Indigenous Australian children.
LINC00473 downregulation facilitates trophoblast cell migration and invasion via the miR‐15a‐5p/LITAF axis in pre‐eclampsia

More and more evidence has identified that long non‐coding RNAs (lncRNAs) are involved in various biological process of numerous diseases. It has been reported that long intergenic non‐protein coding RNA 473 (LINC00473) was associated with pre‐eclampsia (PE) development. However, role and molecular mechanism of LINC00473 in PE remains elusive. Therefore, we designed this research to figure out the specific biological function of LINC00473 in trophoblasts. Firstly, we testified expressions of LINC00473 in trophoblasts of PE with RT‐qPCR analysis. Then, to probe biological function of LINC00473 in trophoblasts of PE, CCK‐8 assay, trans‐well assays and western blot analysis were conducted in Wish and JAR cells. As for verifying interaction of microRNA‐15a‐5p (miR‐15a‐5p) and LINC00473 or lipopolysaccharide induced TNF factor (LITAF), RNA pull‐down and luciferase reporter assays were carried out. Finally, rescue experiments were conducted to probe regulatory pattern of the LINC00473/miR‐15a‐5p/LITAF axis in trophoblasts of PE. As a result, LINC00473 presented a significant upregulation in trophoblasts of PE. Moreover, LINC00473 knockdown induced trophoblast viability, migration, invasion, and epithelial‐to‐mesenchymal transition (EMT) in trophoblasts. Additionally, miR‐15a‐5p interacted with LINC00473 and miR‐15a‐5p was negatively regulated by LINC00473 in trophoblasts. Simultaneously, miR‐15a‐5p negatively modulated LITAF in trophoblasts. Moreover, LITAF overexpression or miR‐15a‐5p downregulation reversed the promotive impact of silenced LINC00473 on trophoblast viability, migration, invasion and EMT. In conclusion, LINC00473 regulated migration and invasion in trophoblasts via the miR‐15a‐5p/LITAF axis. Our study may provide a novel insight for clinical treatment of PE.
Changes in inflammatory gene expression in brain tissue adjacent and distant to a viable cyst in a rat model for neurocysticercosis

Background The parasite Taenia solium causes neurocysticercosis (NCC) in humans and is a common cause of adult-onset epilepsy in the developing world. Hippocampal atrophy, which occurs far from the cyst, is an emerging new complication of NCC. Evaluation of molecular pathways in brain regions close to and distant from the cyst could offer insight into this pathology. Methods Rats were inoculated intracranially with T. solium oncospheres. After 4 months, RNA was extracted from brain tissue samples in rats with NCC and uninfected controls, and cDNA was generated. Expression of 38 genes related to different molecular pathways involved in the inflammatory response and healing was assessed by RT-PCR array. Results Inflammatory cytokines IFN-γ, TNF-α, and IL-1, together with TGF-β and ARG-1, were overexpressed in tissue close to the parasite compared to non-infected tissue. Genes for IL-1A, CSF-1, FN-1, COL-3A1, and MMP-2 were overexpressed in contralateral tissue compared to non-infected tissue. Conclusions The viable cysticerci in the rat model for NCC is characterized by increased expression of genes associated with a proinflammatory response and fibrosis-related proteins, which may mediate the chronic state of infection. These pathways appear to influence regions far from the cyst, which may explain the emerging association between NCC and hippocampal atrophy.
Prevalence of Unruptured Intracranial Aneurysms: A Single Center Experience Using 3T Brain MR Angiography

Purpose The purpose of this study was to evaluate the prevalence and risk factors of unruptured intracranial aneurysms (UIAs), which can help establish guidelines of treatment for asymptomatic Korean adults using 3T magnetic resonance angiography (MRA). Materials and Methods Our Institutional Review Board approved this retrospective study, and informed consent was waived. All patients consisted of healthy individuals who underwent brain MRA using 3T magnetic resonance imaging between January 2011 and December 2012 as part of a routine health examination. Patient data and follow-up results were obtained from medical records. Results A total of 2,118 individuals (mean age=53.9±9.6 years, male:female=1,188:930) who had undergone brain MRA were enrolled in the study. UIAs were found in 80 patients with 105 UIAs (3.77%). Female predominance (55% in UIA vs. 43.47% in non-UIA, P=0.0416) and hypertension were more common in the UIA group (43.75% vs. 28.8%, P=0.004, respectively). The mean size of the aneurysms was 3.10±1.62 mm, and they were all saccular in shape and asymptomatic. The UIAs were most common in the internal carotid artery (59.1%), internal carotid-posterior communicating artery (15.2%), middle cerebral artery (9.5%), anterior communicating artery (8.6%), anterior cerebral artery (4.8%), and vertebral artery (2.9%). Twenty-eight of 80 patients (35%) had multiple aneurysms. The incidence of UIAs increased significantly with age (P=0.014). Conclusion In single center experience, we demonstrated the characteristics and prevalence of UIAs in asymptomatic adults, which may help establish guidelines or therapeutic standards for UIAs.
Financial Maltreatment of Chinese American Elders: An Inquiry through a Cultural Lens

ABSTRACT This study examined financial maltreatment from the perspectives of Chinese American elders via a mixed method approach. Three focus groups of Chinese American elders and one group of service professionals recruited from Phoenix metropolitan areas shared their insights of financial maltreatment and contributed to the refinement of questions in the followed survey that included 325 elders (Mage = 75.6, SD = 7.00). Two types of financial maltreatment: Financial exploitation and financial neglect were identified from focus group discussions. About 9.2% experienced financial exploitation and 1.5% experienced financial neglect. Both financial exploitation and neglect were related to higher vulnerability scores for abuse assessed using the H-S/EAST elder abuse screening scale. Financial maltreatment needs to be understood in legal, cultural, and family contexts, incorporating the perspectives of older adults. Prevention of financial maltreatment hinges on increased awareness and knowledge of this issue among elders, families and service professionals, and the collaborative efforts of stakeholders from ethnic communities.
Reevaluation of the Toxoplasma gondii and Neospora caninum genomes reveals misassembly, karyotype differences, and chromosomal rearrangements

Neospora caninum primarily infects cattle, causing abortions, with an estimated impact of a billion dollars on the worldwide economy annually. However, the study of its biology has been unheeded by the established paradigm that it is virtually identical to its close relative, the widely studied human pathogen Toxoplasma gondii. By revisiting the genome sequence, assembly, and annotation using third-generation sequencing technologies, here we show that the N. caninum genome was originally incorrectly assembled under the presumption of synteny with T. gondii. We show that major chromosomal rearrangements have occurred between these species. Importantly, we show that chromosomes originally named Chr VIIb and VIII are indeed fused, reducing the karyotype of both N. caninum and T. gondii to 13 chromosomes. We reannotate the N. caninum genome, revealing more than 500 new genes. We sequence and annotate the nonphotosynthetic plastid and mitochondrial genomes and show that although apicoplast genomes are virtually identical, high levels of gene fragmentation and reshuffling exist between species and strains. Our results correct assembly artifacts that are currently widely distributed in the genome database of N. caninum and T. gondii and, more importantly, highlight the mitochondria as a previously oversighted source of variability and pave the way for a change in the paradigm of synteny, encouraging rethinking the genome as basis of the comparative unique biology of these pathogens.
COVID‐19‐related consequences on melanoma diagnoses from a local Italian registry in Genoa, Italy

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Adaptive differentiation and rapid evolution of a soil bacterium along a climate gradient

Significance Increasing evidence suggests that evolutionary processes frequently shape ecological patterns; however, most microbiome studies thus far have focused on only the ecological responses of these communities. By using parallel field experiments and focusing in on a model soil bacterium, we showed that bacterial “species” are differentially adapted to local climates, leading to changes in their composition. Furthermore, we detected strain-level evolution, providing direct evidence that both ecological and evolutionary processes operate on annual timescales. The consideration of eco-evolutionary dynamics may therefore be important to understand the response of soil microbiomes to future environmental change. Microbial community responses to environmental change are largely associated with ecological processes; however, the potential for microbes to rapidly evolve and adapt remains relatively unexplored in natural environments. To assess how ecological and evolutionary processes simultaneously alter the genetic diversity of a microbiome, we conducted two concurrent experiments in the leaf litter layer of soil over 18 mo across a climate gradient in Southern California. In the first experiment, we reciprocally transplanted microbial communities from five sites to test whether ecological shifts in ecotypes of the abundant bacterium, Curtobacterium, corresponded to past adaptive differentiation. In the transplanted communities, ecotypes converged toward that of the native communities growing on a common litter substrate. Moreover, these shifts were correlated with community-weighted mean trait values of the Curtobacterium ecotypes, indicating that some of the trait variation among ecotypes could be explained by local adaptation to climate conditions. In the second experiment, we transplanted an isogenic Curtobacterium strain and tracked genomic mutations associated with the sites across the same climate gradient. Using a combination of genomic and metagenomic approaches, we identified a variety of nonrandom, parallel mutations associated with transplantation, including mutations in genes related to nutrient acquisition, stress response, and exopolysaccharide production. Together, the field experiments demonstrate how both demographic shifts of previously adapted ecotypes and contemporary evolution can alter the diversity of a soil microbiome on the same timescale.