Miracle-dz/UniFAR-FTUs huggingface dataset:
Flexible 3D printed microwires and 3D microelectrodes for heart-on-a-chip engineering

We developed a heart-on-a-chip platform that integrates highly flexible, vertical, 3D micropillar electrodes for electrophysiological recording and elastic microwires for the tissue’s contractile force assessment. The high aspect ratio microelectrodes were 3D-printed into the device using a conductive polymer, poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS). A pair of flexible, quantum dots/thermoplastic elastomer nanocomposite microwires were 3D printed to anchor the tissue and enable continuous contractile force assessment. The 3D microelectrodes and flexible microwires enabled unobstructed human iPSC-based cardiac tissue formation and contraction, suspended above the device surface, under both spontaneous beating and upon pacing with a separate set of integrated carbon electrodes. Recording of extracellular field potentials using the PEDOT:PSS micropillars was demonstrated with and without epinephrine as a model drug, non-invasively, along with in situ monitoring of tissue contractile properties and calcium transients. Uniquely, the platform provides integrated profiling of electrical and contractile tissue properties, which is critical for proper evaluation of complex, mechanically and electrically active tissues, such as the heart muscle under both physiological and pathological conditions.
First report of epiphytic dinoflagellate Coolia malayensis (Dinophyceae) in the southeastern Pacific Ocean

Coolia species are epiphytic and benthic dinoflagellates with a cosmopolitan distribution in tropical and subtropical areas. In the austral summer of 2016, during a survey in Bahía Calderilla, a dinoflagellate of the genus Coolia was detected in macroalgae samples, and a clonal culture was established. Subsequently, the cultured cells were observed by scanning electron microscopy (SEM) and identified as C. malayensis based on their morphological characteristics. Phylogenetic analyses based on the LSU rDNA D1/D2 regions confirmed that strain D005‐1 corresponded to C. malayensis and clustered with strains isolated from New Zealand, Mexico, and Asia Pacific countries. Although the strain D005‐1 culture did not contain yessotoxin (YTX), cooliatoxin, 44‐methyl gambierone, or its analogs in detectable amounts by LC–MS/MS, more research is needed to evaluate its toxicity and to determine the possible impact of C. malayensis in northern Chilean waters.
Biofabrication of silver nanoparticles using Spirulina platensis: In vitro anti‐coagulant, thrombolytic and catalytic dye degradation activity

Green synthesis of nanoparticles is an emerging field due to it's biosafety and promising results. Biological systems due to their biodiversity are employed in different production processes. In this study Spirulina platensis mediated silver nanoparticles (S‐AgNPs) production was done. The Uv spectra, FTIR and SEM analysis was performed for the characterization of biosynthesized S‐AgNPs. The biocompatibility evaluation of S‐AgNPs was done through hemolysis analysis. S‐AgNPs were also evaluated for anticoagulant and thrombolytic potential. In addition to the medical applications of S‐AgNPs, silver nanoparticles have been known to show potential industrial applications among which one application is the utilization of silver nanoparticles in the degradation of toxic industrial dyes. Therefore, degradation assay of Eosin Y and Methylene Blue dyes was estimated. The SEM analysis of S‐AgNPs showed the particle size of 50–65 nm, whereas the biocompatibility analysis showed that these S‐AgNPs are biocompatible at ≤400 μM concentration. The S‐AgNPs showed good anticoagulant potential and thrombolytic potential and were able to degrade 44% of the thrombus. The S‐AgNPs significantly degraded 76% of Eosin Y within 30 min, whereas Methylene Blue was 80% degraded within 20 min (P‐value ≤ .001). To the best of our knowledge, the dye degradation of Eosin Y, thrombolytic activity and anticoagulant activity of S‐AgNPs produced from the biomass of Spirulina platensis has been reported for the first time. In the current study, we conclude that our biosynthesized S‐AgNPs showed promising medical and industrial applications and these nanoparticles can be further evaluated and upscaled for large scale applications.
Intranasal oxytocin as a treatment for obesity: safety and efficacy

ABSTRACT Introduction Known for its effect on labor and lactation and on emotional and social functions, oxytocin has recently emerged as a key modulator of feeding behavior and indeed suggested as a potential treatment for obesity. The potential positive effect of oxytocin on both metabolic and psychological-behavioral complications of hypothalamic lesions makes it a promising tool in the management of these conditions. Areas covered The aim of the present review article is to provide an overview of the mechanism of action and clinical experience of the use of oxytocin in different forms of obesity. Expert opinion Current evidence suggests a potential role of oxytocin in the treatment of obesity with different causes. Several challenges remain: an improved understanding of the physiological regulation, mechanisms of action of oxytocin, and interplay with other endocrine axes is fundamental to clarify its role. Further clinical trials are needed to determine the safety and efficacy of oxytocin for the treatment of different forms of obesity. Understanding the mechanism(s) of action of oxytocin on body weight regulation might also improve our understanding of obesity and reveal possible new therapeutic targets – as well as promoting advances in other fields in which oxytocin might be used.
Maternal nutrition during mid-pregnancy and children’s body composition at 7 years of age in the SELMA study

Abstract Optimal nutrition during pregnancy is vital for both maternal and child health. Our objective was to explore if prenatal diet is associated with children’s height and body fat. Nutrient intake was assessed through a FFQ from 808 pregnant women and summarised to a nutrition index, ‘My Nutrition Index’ (MNI). The association with children’s height and body fat (bioimpedance) was assessed with linear regression models. Secondary analysis was performed with BMI, trunk fat and skinfolds. Overall, higher MNI score was associated with greater height (β = 0·47; (95 % CI 0·00, 0·94), among both sexes. Among boys, higher MNI was associated with 0·15 higher BMI z-scores, 0·12 body fat z-scores, 0·11 trunk fat z-scores, and larger triceps, and triceps + subscapular skinfolds (β = 0·05 and β = 0·06; on the log2 scale) (P-value < 0·05). Among girls, the opposite associations were found with 0·12 lower trunk fat z-scores, and smaller subscapular and suprailiac skinfolds (β = −0·07 and β = −0·10; on the log2 scale) (P-value < 0·05). For skinfold measures, this would represent a ± 1·0 millimetres difference. Unexpectedly, a prenatal diet in line with recommended nutrient intake was associated with higher measures of body fat for boys and opposite to girls at a pre-pubertal stage of development.
Indian summer monsoon in future climate projection by a super high-resolution global model

The impact of future climate change on the Indian summer monsoon has been investigated using a super high-resolution global general circulation model. The model with approximately 20-km mesh horizontal resolution can resolve features on finer spatial scales, which were till now resolved by employing high-resolution regional models. Regional models are known to have high dependency on the lateral boundary forcing and significant inability to represent regionalglobal scale interactions comprehensively. Another advantage of the 20-km global model is its fidelity in representing the regional distribution of the present-day monsoon rainfall. Super high-resolution future scenario for the Indian summer monsoon shows widespread but spatially varying increase in rainfall over the interior regions and significant reduction in orographic rainfall over the west coasts of Kerala and Karnataka and the eastern hilly regions around Assam. Over these regions, the drastic reduction of wind by steep orography predominates over the moisture build-up effect (that causes enhanced rainfall over other parts) in reducing the rainfall. This indicates that monsoon rainfall is strongly controlled by parameterized physics and high-resolution processes which need to be resolved with adequately high resolution. The model projects substantial, spatially heterogeneous increase in both extreme hot and heavy rainfall events over most parts of India by the end of the century. While fine-scale surface moisture feedbacks influence the response of extreme hot events, extreme precipitation is influenced by fine-scale orography, evaporation, moisture content and circulation. Thus, the results indicate that consideration of fine-scale processes is critical for accurate assessment of local and regionalscale vulnerability to climate change.
Soluble Guanylyl Cyclase Activator BI 685509 Reduces Portal Hypertension and Portosystemic Shunting in a Rat Thioacetamide-Induced Cirrhosis Model

Portal hypertension (PT) commonly occurs in cirrhosis. Nitric oxide (NO) imbalance contributes to PT via reduced soluble guanylyl cyclase (sGC) activation and cGMP production, resulting in vasoconstriction, endothelial cell dysfunction, and fibrosis. We assessed the effects of BI 685509, an NO-independent sGC activator, on fibrosis and extrahepatic complications in a thioacetamide (TAA)-induced cirrhosis and PT model. Male Sprague–Dawley rats received TAA twice-weekly for 15 weeks (300–150 mg/kg i.p.). BI 685509 was administered daily for the last 12 weeks (0.3, 1, and 3 mg/kg p.o.; n = 8–11 per group) or the final week only (Acute, 3 mg/kg p.o.; n = 6). Rats were anesthetized to measure portal venous pressure. Pharmacokinetics and hepatic cGMP (target engagement) were measured by mass spectrometry. Hepatic Sirius Red morphometry (SRM) and alpha-smooth muscle actin (αSMA) were measured by immunohistochemistry; portosystemic shunting was measured using colored microspheres. BI 685509 dose-dependently increased hepatic cGMP at 1 and 3 mg/kg (3.92 ± 0.34 and 5.14 ± 0.44 versus 2.50 ± 0.19 nM in TAA alone; P < 0.05). TAA increased hepatic SRM, αSMA, PT, and portosystemic shunting. Compared with TAA, 3 mg/kg BI 685509 reduced SRM by 38%, αSMA area by 55%, portal venous pressure by 26%, and portosystemic shunting by 10% (P < 0.05). Acute BI 685509 reduced SRM and PT by 45% and 21%, respectively (P < 0.05). BI 685509 improved hepatic and extrahepatic cirrhosis pathophysiology in TAA-induced cirrhosis. These data support the clinical investigation of BI 685509 for PT in patients with cirrhosis. SIGNIFICANCE STATEMENT BI 685509 is an NO-independent sGC activator that was tested in a preclinical rat model of TAA-induced nodular, liver fibrosis, portal hypertension, and portal systemic shunting. BI 685509 reduced liver fibrosis, portal hypertension, and portal-systemic shunting in a dose-dependent manner, supporting its clinical assessment to treat portal hypertension in patients with cirrhosis.
Effects of mode of transportation on PrEP persistence among urban men who have sex with men

ABSTRACT Little is known about the effect of travel-related factors, such as mode of transportation, on retention in PrEP care, or PrEP persistence. We used data from the 2020 American Men’s Internet Survey and conducted multilevel logistic regression to estimate the association between mode of transportation used for healthcare access and PrEP persistence among urban gay, bisexual, and other men who have sex with men (MSM) in the U.S. MSM using public transportation were less likely to report PrEP persistence (aOR: 0.51; 95% CI: 0.28–0.95) than MSM using private transportation. There were no significant associations between PrEP persistence and using active transportation (aOR: 0.67; 95% CI: 0.35–1.29) or multimodal transportation (aOR: 0.85; 95% CI: 0.51–1.43) compared to using private transportation. Transportation-related interventions and policies are needed to address structural barriers to accessing PrEP services and to improve PrEP persistence in urban areas.
Pressure beneath a traveling wave with constant vorticity

The main focus of this paper is to derive a direct relationship between the surface of an inviscid traveling gravity wave in two dimensions, and the pressure at the bottom of the fluid without approximation, including the effects of constant vorticity. Using this relationship, we reconstruct both the pressure and streamlines throughout the fluid domain. We compare our numerical results with various analytical results (such as the bounds presented in [7-10])as well as known numerical results (see [16]).
Implementation of an Evidence-Based Clinical Guideline for Depression Screening of the Adolescent

Abstract The evidence supports use of a depression screening tool to increase screening completion in the adolescent population. Clinical guidelines include use of the PHQ-9 in the adolescent population (ages 12–18). PHQ-9 screenings are currently lacking in this primary care setting. The aim of this Quality Improvement Project was to improve depression screening in a primary care practice setting located in a rural Appalachian health system. An educational offering with pretest and posttest surveys and a perceived competency scale are utilized. Focus is added to the process and guideline used to complete depression screening. As a result of the QI Project, posttest assessment of knowledge relating to educational offering increased, and use of the screening tool increased by 12.9%. The findings support the importance of education on primary care provider practice and depression screening of adolescents.
ISPD Catheter-related Infection Recommendations: 2023 Update

Peritoneal dialysis (PD) catheter-related infections are important risk factors for catheter loss and peritonitis. The 2023 updated recommendations have revised and clarified definitions and classifications of exit site infection and tunnel infection. A new target for the overall exit site infection rate should be no more than 0.40 episodes per year at risk. The recommendation about topical antibiotic cream or ointment to catheter exit site has been downgraded. New recommendations include clarified suggestion of exit site dressing cover and updated antibiotic treatment duration with emphasis on early clinical monitoring to ascertain duration of therapy. In addition to catheter removal and reinsertion, other catheter interventions including external cuff removal or shaving, and exit site relocation are suggested. Graphical Abstract This is a visual representation of the abstract.
Estimates of Bivalent mRNA Vaccine Durability in Preventing COVID-19–Associated Hospitalization and Critical Illness Among Adults with and Without Immunocompromising Conditions — VISION Network, September 2022–April 2023

On September 1, 2022, CDC's Advisory Committee on Immunization Practices (ACIP) recommended a single bivalent mRNA COVID-19 booster dose for persons aged ≥12 years who had completed at least a monovalent primary series. Early vaccine effectiveness (VE) estimates among adults aged ≥18 years showed receipt of a bivalent booster dose provided additional protection against COVID-19-associated emergency department and urgent care visits and hospitalizations compared with that in persons who had received only monovalent vaccine doses (1); however, insufficient time had elapsed since bivalent vaccine authorization to assess the durability of this protection. The VISION Network* assessed VE against COVID-19-associated hospitalizations by time since bivalent vaccine receipt during September 13, 2022-April 21, 2023, among adults aged ≥18 years with and without immunocompromising conditions. During the first 7-59 days after vaccination, compared with no vaccination, VE for receipt of a bivalent vaccine dose among adults aged ≥18 years was 62% (95% CI = 57%-67%) among adults without immunocompromising conditions and 28% (95% CI = 10%-42%) among adults with immunocompromising conditions. Among adults without immunocompromising conditions, VE declined to 24% (95% CI = 12%-33%) among those aged ≥18 years by 120-179 days after vaccination. VE was generally lower for adults with immunocompromising conditions. A bivalent booster dose provided the highest protection, and protection was sustained through at least 179 days against critical outcomes, including intensive care unit (ICU) admission or in-hospital death. These data support updated recommendations allowing additional optional bivalent COVID-19 vaccine doses for certain high-risk populations. All eligible persons should stay up to date with recommended COVID-19 vaccines.
Clinical trials for neuroregenerative therapies for spinal cord injury: what have we learnt so far?

ABSTRACT Introduction There remains a lack of effective regenerative therapies to treat traumatic spinal cord injury (SCI). On a global level, patients living with SCI, their families, and the healthcare system are impacted by the extensive financial burden associated with the management of SCI. Clinical trials are crucial to assess the real-world efficacy of the different emerging neuroregenerative approaches that have shown promise in the pre-clinical stages of research. Areas covered This perspective summarizes and discusses potential solutions to several key challenges that clinical investigators evaluating novel therapeutic strategies to treat SCI might face: 1) the challenges in patient recruitment and meeting enrollment targets with adequate statistical power; 2) the loss of patients to follow-up; 3) the heterogeneity in patient presentation and recovery trajectories; 4) the complex multifaceted pathophysiology of SCI that renders investigations of single therapeutic approaches difficult; 5) the challenge of capturing positive treatment effects of investigative therapies; 6) the high costs associated with conducting clinical trials; 7) the implementation of current guidelines for treating SCI to optimize care delivery and clinical trial conduct; 8) the shift in SCI patient demographics reflective of an aging population; and 9) navigating regulatory bodies to translate therapies into the clinic. Expert opinion There are challenges when conducting SCI clinical trials that span broadly across medical, social, political, and economic considerations. Thus, we should employ an interdisciplinary approach when addressing these challenges in order to facilitate the evaluation of novel treatments for SCI.
Antenatal size, early childhood growth, and asthma within a cohort created by data linkage

The gestation when small for gestational age (SGA) is first associated with asthma is not well understood. Here, we use routinely acquired data from 10 weeks gestation to up to 28 years of age to test the hypothesis that SGA before birth is associated with an increased risk for asthma in a large population born between 1987 and 2015.
Dietary patterns, obesity markers and leukocyte telomere length among Brazilian civil servants: cross-sectional results from the Pro-Saude study

Abstract Objective: Dietary patterns express the combination and variety of foods in the diet. The partial least squares method allows extracting dietary patterns related to a specific health outcome. Few studies have evaluated obesity-related dietary patterns associated with telomeres length. This study aims to identify dietary patterns explaining obesity markers and to assess their association with leukocyte telomere length (LTL), a biological marker of the ageing process. Design: Cross-sectional study. Setting: University campuses in the state of Rio de Janeiro, Brazil Participants: 478 participants of a civil servants’ cohort study with data on food consumption, obesity measurements (total body fat, visceral fat, BMI, leptin and adiponectin) and blood samples. Results: Three dietary patterns were extracted: (1) fast food and meat; (2) healthy and (3) traditional pattern, which included rice and beans, the staple foods most consumed in Brazil. All three dietary patterns explained 23·2 % of food consumption variation and 10·7 % of the obesity-related variables. The fast food and meat pattern were the first factor extracted, explaining 11–13 % variation of the obesity-related response variables (BMI, total body fat and visceral fat), leptin and adiponectin showed the lowest percentage (4·5–0·1 %). The healthy pattern mostly explained leptin and adiponectin variations (10·7 and 3·3 %, respectively). The traditional pattern was associated with LTL (β = 0·0117; 95 % CI 0·0001, 0·0233) after adjustment for the other patterns, age, sex, exercise practice, income and energy intake. Conclusion: Leukocyte telomere length was longer among participants eating a traditional dietary pattern that combines fruit, vegetables and beans.
On the Ordering of Pooled Web Pages, Gold Assessments, and Bronze Assessments

The present study leverages a recent opportunity we had to create a new English web search test collection for the NTCIR-16 We Want Web (WWW-4) task, which concluded in June 2022. More specifically, through the test collection construction effort, we examined two factors that may affect the relevance assessments of depth-k pools, which in turn may affect the relative evaluation of different IR systems. The first factor is the document ordering strategy for the assessors, namely, prioritisation (PRI) and randomisation (RND). PRI is a method that has been used in NTCIR tasks for over a decade; it ranks the pooled documents by a kind of pseudorelevance for the assessors. The second factor is assessor type, i.e., Gold or Bronze. Gold assessors are the topic creators and therefore they “know” which documents are (highly) relevant and which are not; Bronze assessors are not the topic creators and may lack sufficient knowledge about the topics. We believe that our study is unique in that the authors of this article served as the Gold assessors when creating the WWW-4 test collection, which enabled us to closely examine why Bronze assessments differ from the Gold ones. Our research questions examine assessor efficiency (RQ1), inter-assessor agreement (RQ2), system ranking similarity with different qrels files (RQ3), system ranking robustness to the choice of test topics (RQ4), and the reasons why Bronze assessors tend to be more liberal than Gold assessors (RQ5). The most remarkable of our results are as follows: First, in the comparisons for RQ1 through RQ4, it turned out that what may matter more than the document ordering strategy (PRI vs. RND) and the assessor type (Gold vs. Bronze) is how well-motivated and/or well-trained the Bronze assessors are. Second, regarding RQ5, of the documents originally judged nonrelevant by the Gold assessors contrary to the Bronze assessors in our experiments, almost one half were truly relevant according to the Gold assessors’ own reconsiderations. This result suggests that even Gold assessors are far from perfect; budget permitting, it may be beneficial to hire highly motivated Bronze assessors in addition to Gold assessors so they can complement each other.