Miracle-dz/UniFAR-FTUs huggingface dataset:
When Mechanical Stress Matters: Generation of Polyploid Giant Cancer Cells in Tumor-like Microcapsules

In this work, we studied the generation and rising of polyploid cancer cells as a product of mechanical stress. To this purpose, MCF7 breast cancer cells were cultured on 2D (i.e. flasks, or flat hydrogels), and in 3D milieus (i.e. Spheroids, or immobilized within alginate-gelatin microbeads, named in this work as tumor-like microcapsules), and further analyzed by biophysical and genetic methods (i.e. single-cell Traction Force Microscopy and RNA-seq respectively). Our results show that MCF7 cells preconditioned onto 2D surfaces exhibit a low number of polynucleated cells, while their culture in 3D environments triggered their progressive generation with time. Genetic studies enabled us to determine that polyploid cells found in tumor-like microcapsules are likely originated by cell-cell fusion and disrupted cytokinesis, showing most of the genetic markers for Polyploid Giant Cancer Cell, while cells cultured as spheroids seem to be likely generated by other mechanisms, such as cell cannibalisms, entosis, or emperipolesis. Our outcomes strongly suggest that both mechanical stress and confinement are required to stimulate cell polyploidy, which can be easily addressed by the immobilization of breast cancer cells in tumor-like microcapsules.
BRIGHTEST CLUSTER GALAXIES AT THE PRESENT EPOCH

We have obtained photometry and spectroscopy of 433 z ⩽ 0.08 brightest cluster galaxies (BCGs) in a full-sky survey of Abell clusters to construct a BCG sample suitable for probing deviations from the local Hubble flow. The BCG Hubble diagram over 0 < z < 0.08 is consistent to within 2% of the Hubble relation specified by a Ωm = 0.3, Λ = 0.7 cosmology. This sample allows us to explore the structural and photometric properties of BCGs at the present epoch, their location in their hosting galaxy clusters, and the effects of the cluster environment on their structure and evolution. We revisit the Lm–α relation for BCGs, which uses α, the log-slope of the BCG photometric curve of growth, to predict the metric luminosity in an aperture with 14.3 kpc radius, Lm, for use as a distance indicator. Residuals in the relation are 0.27 mag rms. We measure central stellar velocity dispersions, σ, of the BCGs, finding the Faber–Jackson relation to flatten as the metric aperture grows to include an increasing fraction of the total BCG luminosity. A three-parameter “metric plane” relation using α and σ together gives the best prediction of Lm, with 0.21 mag residuals. The distribution of projected spatial offsets, rx of BCGs from the X-ray-defined cluster center is a steep γ = −2.33 power law over 1 < rx < 103 kpc. The median offset is ∼10 kpc, but ∼15% of the BCGs have rx > 100 kpc. The absolute cluster-dispersion normalized BCG peculiar velocity |ΔV1|/σc follows an exponential distribution with scale length 0.39 ± 0.03. Both Lm and α increase with σc. The α parameter is further moderated by both the spatial and velocity offset from the cluster center, with larger α correlated with the proximity of the BCG to the cluster mean velocity or potential center. At the same time, position in the cluster has little effect on Lm. Likewise, residuals from the metric plane show no correlation with either the spatial or velocity offset from the cluster center. The luminosity difference between the BCG and second-ranked galaxy, M2, increases as the peculiar velocity of the BCG within the cluster decreases. Further, when M2 is a close luminosity “rival” of the BCG, the galaxy that is closest to either the velocity or X-ray center of the cluster is most likely to have the larger α. We conclude that the inner portions of the BCGs are formed outside the cluster, but interactions in the heart of the galaxy cluster grow and extend the envelopes of the BCGs.
The transcriptome of acute dehydration in Myeloid Leukemia cells

Live human myeloid leukemia (HL-60/S4) cells exposed to acute hyperosmotic stress with sucrose undergo dehydration and cell shrinkage. Interphase chromatin and mitotic chromosomes congeal, and exhibit altered phase separation (demixing) of chromatin-associated proteins. To investigate concurrent changes in the transcriptome, we exposed exponentially growing HL-60/S4 cells to acute hyperosmotic stress (∼600 milliOsmolar) for 30 and 60 minutes by addition of sucrose to the culture medium. We employed RNA-Seq of polyA mRNA to identify genes with significantly increased or decreased transcript levels relative to untreated control cells (i.e., differential gene expression). These identified genes were examined for over-representation of Gene Ontology (GO) terms. In hyperosmotically-stressed cells, multiple GO terms associated with transcription, translation, mitochondrial function and proteosome activity, as well as the gene set “replication-dependent histones”, were over-represented among genes with increased transcript levels; whereas, genes with decreased transcript levels were over-represented in various GO terms for transcription repressors. The overall transcriptome profiles of these stressed cells suggest a rapid acquisition of cellular rebuilding, a futile homeostatic response, as these cells are ultimately doomed to a dehydrated death. “Do not go gentle into that good night Rage, rage against the dying of the light” Dylan Thomas (1914-1953)
Conformational dynamics and target-dependent myristoyl switch of calcineurin B homologous protein 3

Calcineurin B homologous protein 3 (CHP3) is an EF-hand Ca2+-binding protein involved in regulation of cancerogenesis, cardiac hypertrophy and neuronal development via interactions with sodium/proton exchangers (NHEs) and signalling proteins. CHP3 binds Ca2+ with micromolar affinity providing the basis to respond to intracellular Ca2+ signals. Ca2+ binding and myristoylation are important for CHP3 function but the underlying molecular mechanism remained elusive. Here, we show that Ca2+ binding and myristoylation independently affect conformational dynamics and functions of human CHP3. Ca2+ binding increased flexibility and hydrophobicity of CHP3 indicative of an open conformation. CHP3 in open Ca2+-bound conformation had higher affinity for NHE1 and associated stronger with lipid membranes compared to the closed Mg2+-bound conformation. Myristoylation enhanced flexibility of CHP3 and decreased its affinity to NHE1 independently of the bound ion, but did not affect its binding to lipid membranes. The data exclude the proposed Ca2+-myristoyl switch for CHP3. Instead, they document a Ca2+-independent exposure of the myristoyl moiety induced by binding of the target peptide to CHP3 enhancing its association to lipid membranes. We name this novel regulatory mechanism “target-dependent myristoyl switch”. Taken together, the interplay of Ca2+ binding, myristoylation and target binding allows for a context-specific regulation of CHP3 functions.
Study on the mechanism of HE4 in hyperoxia-induced alveolar damage in rats

Objective This study investigated the mechanism of human epididymis protein 4 (HE4) in hyperoxia-induced alveolar damage in rats. Methods The pathological changes of SD rat lung tissue were detected by hematoxylin-eosin staining. Plasma protein levels were measured by enzyme-linked immunosorbent assay (ELISA). The expression of mRNA was detected by real-time RT-PCR. Results Hyperoxia intervention within 7 days could induce acute lung injury in neonatal SD rats. Hyperoxia induction can increase HE4, MMP9 and TIMP1 in plasma and tissue of neonatal SD rats. By overexpressing and silencing HE4 gene in neonatal rat primary alveolar type II epithelial cells, we found that HE4 protein activates the phosphorylation of ERK and p65, activates the downstream MMP9 signaling pathway, inhibits MMP9 mRNA expression, inhibits protein activity, and reduces COLI degradation, increases collagen secretion, promoting matrix remodeling and fibrosis. Conclusion HE4 mediates the pathophysiological process of hyperoxia-induced lung injury in rats through ERK, MMP9 and TIMP1 signaling pathways. HE4 overexpression mediates lung basal remodeling and lung injury.
Studying Shared Regulation in Immersive Learning Environments

: We examined the regulation of shared problem solving in a museum exhibit. We found that we had to augment our dialogue codes to properly embrace the dynamic nature of the observed learning regulation. These changes reflect aspects of shared regulation that occur when learning takes place (1) in an immersive open-ended learning environment, where (2) learners work together in large groups. We present preliminary results, arguing that designers and researchers may benefit from recognizing how planning and evaluation acts can be tactically embedded in immersive learning environments.
The Experiencing Scale: An Experiential Learning Gauge of Engagement in Learning

A major premise of experiential learning practices is that experience is necessary for learning, yet our understanding of the concept of experience and its role in learning remains unclear. This study examines the experiencing process in experiential learning and formulates a conceptual foundation for the experiencing concept that integrates insights from four contemporary traditions of experiencing research: Focusing, Flow, Mindfulness and Absorption. Empirical validation is tested with the construction of The Experiencing Scale, a self-reported gauge of one’s level of experiencing in a given context. The Experiencing Scale instrument was distributed to undergraduate students following participation in an experiential classroom activity. Analysis was conducted using structural equation modeling to determine validity and reliability of the Experiencing Scale. From a sample of 270, our findings identified 18 items, which grouped to represent three factors, Novelty, Presence and Embodiment. The results suggest that when these factors are experienced, learners can more fully engage in all stages of the experiential learning cycle (Kolb, 2015). While exploratory in nature, the scale can be a useful tool for those who wish to increase the level of experiencing and engagement in learners and potentially serve as a guide to the design, implementation, and evaluation of experiential learning.
Corners in dense subsets of P^d

Let $\PP^d$ be the $d$-fold direct product of the set of primes. We prove that if $A$ is a subset of $\PP^d$ of positive relative upper density then $A$ contains infinitely many "corners", that is sets of the form $\{x,x+te_1,. ,x+te_d\}$ where x is an integer point and e_1,. ,e_d are the standard basis vectors of the d-dimensional Euclidean space. Our argument is based on proving a removal lemma for weighted uniform hypergraphs, where the weight system is defined in terms of a pairwise linearly independent family of linear forms.
Literature screening report Secondary health impact of COVID-19 containment measures in children, adolescents and young adults

Given the rather small number of studies that we have identified so far in our literature search, we refer to the summaries of the respective questions for an overview of the current findings. In the next report, there will be a larger number of studies and we will complement the summaries of the questions with a concise abstract. The same study investigated nutritional behaviors . Results can be summarized accordingly: Changes in food consumption differ by food type. General increase in legumes and fruit, and sweet foods and beverages, while no change in processed meat and decrease in fast food. Associated factors like gender, maternal education and family size vary regarding the impact on food consumption. An Italian study (Pietrobelli et al, 2020) in obese children confirms changes, however, not fully consistent with Ruiz-Roso et al., as they did not find change in legumes and fruit but change in meat consumption. In a study on diabetics, more than half of patients (56.9%) did not change their eating habits during the lock-down period, while 26.5% increased carbohydrate consumption, 7.8% and 8.8% ate a large amount of fat and protein, respectively.
Critical thinking and inclusive practice: A qualitative study of Spanish primary school teachers' perceptions

As one of the most necessary skills of the 21st century for problem solving, critical thinking should be taught and included in the curriculum of those schools in which inclusivity towards all their students is a priority. From this perspective, this study presents the educational implications for the teaching and evaluation of this skill. In addition, teachers' perceptions regarding the teaching methods used, evaluation techniques and limitations that they encounter at the moment of enhancing the critical thinking of their students are analysed. A descriptive-comprehensive research with a qualitative approach was adopted, with data collected from interviews with 10 primary education teachers in the Spanish educational system. The analysis suggests that, although there is some knowledge on the part of teachers about critical thinking skills, most of them are not able to respond to this learning need. They highlight the technique of joint discussion and interactions between teams as among the effective tools for fostering critical thinking skills. Notably, these teachers have referred to the existence of certain relationships between critical thinking and equitable and quality education.
Novel and diverse mycoviruses co-infecting a single strain of the phytopathogenic fungus Alternaria dianthicola

Alternaria dianthicola is a pathogenic fungus that causes serious leaf or flower blight on some medicinal plants worldwide. In this study, multiple dsRNA bands in the range of 1.2-10 kbp were found in a Alternaria dianthus strain HNSZ-1, and eleven full-length cDNA sequences of these dsRNA were obtained by high-throughput sequencing, RT-PCR detection and conventional Sanger sequencing. Homology search and phylogenetic analyses indicated that the strain HNSZ-1 was infected by at least nine mycoviruses. Among the nine, five viruses were confirmed to represent novel viruses in the families Hypoviridae, Totiviridae, Mymonaviridae and a provisional family Ambiguiviridae. Virus elimination and horizontal transmission indicated that the (-) ssRNA virus, AdNSRV1, might be associated with the slow growth and irregular colony phenotype of the host fungus. As far as we know, this is the first report for virome characterization of A. dianthus, which might provide important insights for screening of mycovirus for biological control and for studying of the interactions between viruses or viruses and their host.
Family-supportive supervisor behaviors and career sustainability of e-commerce female workers: A mixed-method approach

Women play an essential role in promoting societal and economic harmony development. However, compared with their male counterparts, female employees usually have to take on more family responsibilities while they endeavor to perform well at work. It is inevitable for them to face work–family conflicts; therefore, how to make female employees' careers more sustainable is a critical concern. Even though female career sustainability is well-explored in the literature, the combined effect of worker self-efficacy and family-supportive supervisor behaviors (FSSBs) on female career sustainability remains unexplored. To fill this gap, this study examines the influence of FSSB on female employee career sustainability, as well as the mediating role of self-efficacy. Moreover, a mixed-method approach was used to test the proposed relationships. The results indicate that FSSB has a positive impact on female career sustainability. Furthermore, the findings revealed that FSSB promotes female career sustainability through self-efficacy. This study establishes a theoretical framework for further research on the relationships between leader behavior and employee career sustainability. At the same time, it offers practical implications for supervisors in the management of subordinate career sustainability.
Spatial and temporal analysis of liver cancer mortality in Yunnan province, China, 2015–2019

Liver cancer is a major public health challenge. Few published studies reported temporal trend and geographical distribution of liver cancer mortality in China, especially in less developed southwest regions with higher liver cancer incidence. In the current study, we obtained liver cancer mortality data from population-based death surveillance system in Yunnan province in 2015–2019. The mortality of liver cancer was analyzed by using the joinpoint regression model. The space distribution of liver cancer mortality in 129 counties and districts in Yunnan province was illustrated by using the ArcGIS software. Moran's I method was used to estimate the global and local spatial autocorrelation of liver cancer mortality. Analytical results revealed that from 2015 to 2019, the average mortality rate of liver cancer in Yunnan province was 12.96/100,000, with an average annual growth rate of 6.26% (p < 0.05). Higher liver cancer mortality was found in rural areas and in males. Moreover, people aged 45–50 years experienced a steep increase in liver cancer mortality rate. High-high cluster was mainly consisted of areas with higher hepatitis virus infection rate or severe intravenous drug use problem. Our study results suggest a heavy burden of liver cancer in southwest China Yunnan province. Comprehensive intervention measures need to be developed and implemented.
Superconducting qubit manipulated by fast pulses: experimental observation of distinct decoherence regimes

A particular superconducting quantum interference device (SQUID) qubit, namely the double SQUID qubit, can be manipulated by rapidly modifying its potential with the application of fast flux pulses. In this system we observe coherent oscillations exhibiting non-exponential decay, indicating an unconventional decoherence mechanism. Moreover, via tuning the qubit in different conditions (different oscillation frequencies) by changing the pulse height, we observe a crossover between two distinct decoherence regimes and the existence of an ‘optimal’ point where the qubit is only weakly sensitive to intrinsic noise. We find that this behavior is in agreement with a model considering the decoherence caused essentially by low-frequency noise contributions, and we discuss the experimental results and possible issues.
Role of Interleukin-1 family in bone metastasis of prostate cancer

Prostate cancer (PCa) is one of the most fatal diseases in male patients with high bone metastatic potential. Bone metastasis severely shortens overall survival and brings skeletal-related events (SREs) which reduces the life quality of patients, and this situation is currently regarded as irreversible and incurable. The progression and metastasis of PCa are found to be closely associated with inflammatory cytokines and chemokines. As pivotal members of inflammatory cytokines, Interleukin-1 (IL-1) family plays a crucial role in this process. Elevated expression of IL-1 family was detected in PCa patients with bone metastasis, and accumulating evidences proved that IL-1 family could exert vital effects on the progression and bone metastasis of many cancers, while some members have dual effects. In this review, we discuss the role of IL-1 family in the bone metastasis of PCa. Furthermore, we demonstrate that many members of IL-1 family could act as pivotal biomarkers to predict the clinical stage and prognosis of PCa patients. More importantly, we have elucidated the role of IL-1 family in the bone metastasis of PCa, which could provide potential targets for the treatment of PCa bone metastasis and probable directions for future research.
The mediating effect of subjective well-being in the relationship between social support and professional commitment among mainland Chinese kindergarten teachers

Kindergarten teachers’ professional commitment affects their emotional input and turnover intention, and it is affected by the spiritual and material factors of teachers’ families, kindergartens, and society. Therefore, this study aimed to investigate the mediating effect of the dimensions of subjective well-being in the relationship between social support and professional commitment. The study is grounded in human ecology theory and social exchange theory. We surveyed 778 kindergarten teachers from different educational systems in Guangdong Province in China. We used the “Appreciative Social Support” and “Subjective Well-being” Scales and the “Professional Commitment Questionnaire. ” The results showed that the four variables of social support, positive affect, life satisfaction, and professional commitment of kindergarten teachers were significantly and positively correlated with each other: social support positively predicted positive affect and life satisfaction, positive affect and life satisfaction positively predicted professional commitment, and social support indirectly influenced professional commitment through the parallel and chain path of positive affect and life satisfaction. This represents a compound multiple mediating effects on professional commitment.
Inhibition of gastric acid secretion with omeprazole affects fish specific dynamic action and growth rate: Implications for the development of phenotypic stomach loss

An acid-secreting stomach provides many selective advantages to fish and other vertebrates; however, phenotypic stomach loss has occurred independently multiple times and is linked to loss of expression of both the gastric proton pump and the protease pepsin. Reasons underpinning stomach loss remain uncertain. Understanding the importance of gastric acid-secretion to the metabolic costs of digestion and growth will provide information about the metabolic expense of acid-production and performance. In this study, omeprazole, a well characterized gastric proton pump inhibitor, was used to simulate the agastric phenotype by significantly inhibiting gastric acidification in Nile tilapia. The effects on post-prandial metabolic rate and growth were assessed using intermittent flow respirometry and growth trials, respectively. Omeprazole reduced the duration (34.4%) and magnitude (34.5%) of the specific dynamic action and specific growth rate (21.3%) suggesting a decrease in digestion and assimilation of the meal. Gastric pH was measured in control and omeprazole treated fish to confirm that gastric acid secretion was inhibited for up to 12 h post-treatment (p < 0.05). Gastric evacuation measurements confirm a more rapid emptying of the stomach in omeprazole treated fish. These findings reinforce the importance of stomach acidification in digestion and growth and present a novel way of determining costs of gastric digestion.