Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of the antimicrobial effects of Capsicum, Nigella sativa, Musa paradisiaca L., and Citrus limetta: A review

The extensive use of antibiotics and vaccines against microbial infections can result in long-term negative effects on humans and the environment. However, there are a number of plants that have antimicrobial effects against various disease-causing microbes such as bacteria, viruses, and fungi without negative side effects or harm to the environment. In this regard, four particular plants- Capsicum, Nigella sativa, Musa paradisiaca L. , and Citrus limetta have been widely considered due to their excellent antimicrobial effect and ample availability. In this review, we discuss their antimicrobial effects due to the presence of thymoquinone, p-cymene, pinene, alkaloids, limonene, camphene, and melanin. These antimicrobial compounds disrupt the cell membrane of microbes, inhibit cellular division, and form biofilm in bacterial species, eventually reducing the number of microbes. Extraction of these compounds from the respective plants is carried out by different methods such as soxhlet, hydro-distillation, liquid-liquid extraction (LLE), pressurized liquid extraction (PLE), solid-phase extraction (SPE), supercritical fluid extraction (SFE), pulsed electric field (PEF), microwave-assisted extraction (MAE), enzyme-assisted extraction (EAE), ultrasound-assisted extraction (UAE), and high-voltage electrical discharge. Suitable selection of the extraction technique highly depends upon the associated advantages and disadvantages. In order to aid future study in this field, this review paper summarizes the advantages and disadvantages of each of these approaches. Additionally, the discussion covers how antimicrobial agents destroy harmful bacteria. Thus, this review offers in-depth knowledge to researchers on the antibacterial properties of Capsicum, Nigella sativa, Musa paradisiaca L. peels, and Citrus limetta.
Reduced salinity exacerbates the viral infection on the coccolithophorid Emiliania huxleyi at elevated pCO2

The blooms of coccolithophores are usually terminated by their viruses. However, little has been documented on the interplay between the host with virus under different environmental conditions. We investigated the relationship of Emiliania huxleyi (BOF92) and its virus (EhV99B1) after the coccolithophorid had acclimated to different levels of salinity (S35: 35‰; S25: 25‰) and CO2 (AC: 400 μatm; HC: 1000 μatm) for about 15 generations. Our results showed that the virus infection decreased the growth of E. huxleyi along with decreased photochemical efficiency and photosynthetic carbon fixation under all the combinations of the treatments. Lowered salinity exacerbated the impacts of the viral infection on the growth and photosynthetic performance of E. huxley. The elevated pCO2 appeared to have alleviated the impacts of the viral infection on its photosynthetic performance with enhanced levels of Fv/Fm , by about 49% under S35 and by about 16% under S25, respectively. Nevertheless, the elevated pCO2 enhanced the virus burst size by about 18% under the high and by about 46% under the low levels of salinity, respectively. It is concluded that reduced salinity under the elevated pCO2 exacerbates the impact of the virus on E. huxleyi, leading to the highest burst sizes and the lowest photosynthetic carbon fixation, and the enhanced photochemical efficiency and increased levels of Chl a under the elevated pCO2 could have facilitated the energy supply for the virus multiplication in the infected cells of E. huxleyi.
Perceptional differences in the factors of local acceptance of waste incineration plant

Existing research has documented that public attitudes towards waste incineration plants are determined by various factors, such as risk perception, economic impacts, and social trust. However, the diversity in perceptions within communities hosting waste incineration plants is understudied. Adds to existing knowledge, the present paper employed the Q methodology to examine the perceptions of residents living in the vicinity of a waste incineration plant in Xuzhou, China. The results revealed four perspectives on residents’ perceptions towards waste incineration plants: I do not trust them and feel besieged by risks; I trust local governments but I am unfairly treated; I attach this place a lot but I am unfairly treated; I possess knowledge of waste incineration and feel besieged by risks. Our data show that risk perception, trust perception, and political efficacy perception are underlying reasons for local acceptance of waste incineration plants. The diversified subjectivities we obtained supplement existing literature that quantitatively documents the influencing factors. These findings demonstrate that it is necessary to explicitly consider the deep-seated values and perspectives among hosting residents’ for the siting of the waste incineration plant.
Correlation of safety behavior, handover quality, and risk perception: A cross-sectional study among Chinese psychiatric nurses

Background Nurses’ safety behaviors played an important role in patients’ safety goal realization, and it varies from person to person. However, less research has explored the safety behavior level of psychiatric nurses and its influencing factors. Thus, this research aimed to assess the level of safety behavior and explore whether risk perception mediated the relationship between handover quality and safety behavior among psychiatric nurses. Methods A total of 186 registered psychiatric nurses in a Chinese hospital were recruited for this study, through the convenience sampling method. Handover quality, risk perception, and safety behavior were measured. Hayes’ PROCESS macro was used to evaluate the mediation of risk perception between handover quality and safety behavior. Results Scores of psychiatric nurses’ safety behaviors were (47.98 ± 7.45), and handover quality and risk perception could predict the variance of nurses’ safety behaviors. Risk perception could partially mediate between handover quality and nurses’ safety behaviors, and the value of the mediating effect was 49.17%. Conclusion Psychiatric nurses’ safety behaviors have a large promotion space. Therefore, healthcare professionals should endeavor to improve the handover quality of psychiatric nurses and decrease their risk perception, thereby promoting nurses’ safety behaviors.
Enteral supplementation with probiotics in preterm infants: A retrospective cohort study and 6-year follow-up

The objective of this retrospective cohort study was to assess the impact of an enteral probiotics supplementation protocol on the incidence of necrotizing enterocolitis (NEC) in infants born <33 weeks gestational age (GA) or birth weight (BW) <1,500 g. In addition, a 6-year follow-up is presented after instigation of probiotic use. In October 2014, our NICU introduced an enteral probiotics supplementation protocol for infants born <33 weeks GA or BW <1,500 g. Infants received 0.5 g of Bifidobacterium breve HA-129, Lacticaseibacillus rhamnosus HA-111, Bifidobacterium bifidum HA-132, Bifidobacterium longum subsp. infantis HA-116, and Bifidobacterium longum subsp. longum HA-135 (FloraBABYⓇ) daily until discharge or transfer from hospital. The incidence of NEC was compared among infants for 2 years pre- and post implementation of the protocol then 6-years following continuous implementation of the probiotic use. In total, 370 infants not treated with probiotics between 2012 and 2014 were included with an incidence of NEC at 4.9%. In comparison, the 367 infants who received had a 67% reduction (4.9–1.6%, p = 0.01) in our Neonatal Intensive Care Unit (NICU). The results remained significant (aOR = 0.26; 95% CI: 0.09, 0.72; p < 0.01) after adjusting for GA, small for gestational age, and antenatal corticosteroid use. Data from the Canadian Neonatal Network not only showed a consistently high rate of NEC in October 2014, but also identified exceedingly high rates (8.7–15.6%) in some hospitals up to 2021, while our rates have been consistently low with using the probiotic as standard therapy for low BW premature babies, with no serious side effects reported. In conclusion, the introduction of a five-strain probiotic natural health product has coincided with a reduced the incidence and complications of NEC in our NICU setting.
Methotrexate inhibition of SARS-CoV-2 entry, infection and inflammation revealed by bioinformatics approach and a hamster model

Background Drug repurposing is a fast and effective way to develop drugs for an emerging disease such as COVID-19. The main challenges of effective drug repurposing are the discoveries of the right therapeutic targets and the right drugs for combating the disease. Methods Here, we present a systematic repurposing approach, combining Homopharma and hierarchal systems biology networks (HiSBiN), to predict 327 therapeutic targets and 21,233 drug-target interactions of 1,592 FDA drugs for COVID-19. Among these multi-target drugs, eight candidates (along with pimozide and valsartan) were tested and methotrexate was identified to affect 14 therapeutic targets suppressing SARS-CoV-2 entry, viral replication, and COVID-19 pathologies. Through the use of in vitro (EC50 = 0.4 μM) and in vivo models, we show that methotrexate is able to inhibit COVID-19 via multiple mechanisms. Results Our in vitro studies illustrate that methotrexate can suppress SARS-CoV-2 entry and replication by targeting furin and DHFR of the host, respectively. Additionally, methotrexate inhibits all four SARS-CoV-2 variants of concern. In a Syrian hamster model for COVID-19, methotrexate reduced virus replication, inflammation in the infected lungs. By analysis of transcriptomic analysis of collected samples from hamster lung, we uncovered that neutrophil infiltration and the pathways of innate immune response, adaptive immune response and thrombosis are modulated in the treated animals. Conclusions We demonstrate that this systematic repurposing approach is potentially useful to identify pharmaceutical targets, multi-target drugs and regulated pathways for a complex disease. Our findings indicate that methotrexate is established as a promising drug against SARS-CoV-2 variants and can be used to treat lung damage and inflammation in COVID-19, warranting future evaluation in clinical trials.
The function of p53 and its role in Alzheimer’s and Parkinson’s disease compared to age-related macular degeneration

The protein p53 is the main human tumor suppressor. Since its discovery, extensive research has been conducted, which led to the general assumption that the purview of p53 is also essential for additional functions, apart from the prevention of carcinogenesis. In response to cellular stress and DNA damages, p53 constitutes the key point for the induction of various regulatory processes, determining whether the cell induces cell cycle arrest and DNA repair mechanisms or otherwise cell death. As an implication, aberrations from its normal functioning can lead to pathogeneses. To this day, neurodegenerative diseases are considered difficult to treat, which arises from the fact that in general the underlying pathological mechanisms are not well understood. Current research on brain and retina-related neurodegenerative disorders suggests that p53 plays an essential role in the progression of these conditions as well. In this review, we therefore compare the role and similarities of the tumor suppressor protein p53 in the pathogenesis of Alzheimer’s (AD) and Parkinson’s disease (PD), two of the most prevalent neurological diseases, to the age-related macular degeneration (AMD) which is among the most common forms of retinal degeneration.
The Xbp1-regulated transcription factor Mist1 restricts antibody secretion by restraining Blimp1 expression in plasma cells

Antibody secretion by plasma cells provides acute and long-term protection against pathogens. The high secretion potential of plasma cells depends on the unfolded protein response, which is controlled by the transcription factor Xbp1. Here, we analyzed the Xbp1-dependent gene expression program of plasma cells and identified Bhlha15 (Mist1) as the most strongly activated Xbp1 target gene. As Mist1 plays an important role in other secretory cell types, we analyzed in detail the phenotype of Mist1-deficient plasma cells in Cd23-Cre Bhlha15 fl/fl mice under steady-state condition or upon NP-KLH immunization. Under both conditions, Mist1-deficient plasma cells were 1.4-fold reduced in number and exhibited increased IgM production and antibody secretion compared to control plasma cells. At the molecular level, Mist1 regulated a largely different set of target genes compared with Xbp1. Notably, expression of the Blimp1 protein, which is known to activate immunoglobulin gene expression and to contribute to antibody secretion, was 1.3-fold upregulated in Mist1-deficient plasma cells, which led to a moderate downregulation of most Blimp1-repressed target genes in the absence of Mist1. Importantly, a 2-fold reduction of Blimp1 (Prdm1) expression was sufficient to restore the cell number and antibody expression of plasma cells in Prdm1 Gfp/+ Cd23-Cre Bhlha15 fl/fl mice to the same level seen in control mice. Together, these data indicate that Mist1 restricts antibody secretion by restraining Blimp1 expression, which likely contributes to the viability of plasma cells.
A study of factors influencing Chinese college students’ intention of using metaverse technology for basketball learning: Extending the technology acceptance model

Introduction Based on the expansion of flow constructs based on the TAM model, this study assesses the impact of metaverse technology in college basketball courses. Methods We surveyed 849 effective samples using an online questionnaire survey, verified our analysis using structural equation modeling, and examined the moderating effect of gender on the path relationship. Results The perceived ease of use, the flow experience, and the perceived usefulness of the product are important predictors of behavioral intention. According to the study, perceived usefulness, and flow experience influence attitudes significantly. A moderating effect of gender is observed on perceived ease of use on the path to behavioral intention, and the results extend the theoretical research on the use of metaverse technology for basketball instruction and TAM. Discussion A metaverse-based learning experience can enhance the flow experience of basketball learning, thus increasing the willingness to use and the effectiveness of learning.
Serum metabolic signatures of schizophrenia patients complicated with hepatitis B virus infection: A 1H NMR-based metabolomics study

Introduction Schizophrenia (SZ) is a severe chronic mental disorder with increased risk of hepatitis B virus (HBV) infection, which is incurable currently and induces various negative emotions and psychological pressures in patients to exacerbate mental disorders. To facilitate the therapeutic design for SZ patients complicated with HBV infection (SZ + HBV), it is helpful to first elucidate the metabolic perturbations in SZ + HBV patients. Methods In this study, metabolic profiles of the serum samples from four groups of participants comprising healthy controls (HC, n = 72), HBV infection (n = 52), SZ patients (n = 37), and SZ + HBV (n = 41) patients were investigated using a high-resolution 1H NMR-based metabolomics approach. Results and discussion Distinguishable metabolic profiles were found in the four groups. In comparison with HC, HBV infection induced increased levels of citrate and succinate to perturbate the tricarboxylic acid cycle and succinate-related pathways. Similar to SZ cases, SZ + HBV patients exhibited decreased glucose but increased citrate, pyruvate, and lactate, suggesting the occurrence of disturbance in glucose metabolism. Moreover, in comparison with HC, several serum amino acid levels in SZ + HBV patients were significantly altered. Our findings suggest that Warburg effect, energy metabolism disorders, neurotransmitter metabolism abnormalities, mitochondrial dysfunction and several disturbed pathways in relation to tyrosine and choline appear to play specific and central roles in the pathophysiology of SZ + HBV. Apart from replicating metabolic alterations induced by SZ and HBV separately (e.g., in energy metabolism and Warburg effect), the specific metabolic abnormalities in the SZ + HBV group (e.g., several tyrosine- and choline-related pathways) highlighted the existence of a synergistic action between SZ and HBV pathologies. Current study revealed the metabolic alterations specific to the interaction between SZ and HBV pathologies, and may open important perspectives for designing precise therapies for SZ + HBV patients beyond the simple combination of two individual treatments.
Impact of objectively-measured sleep duration on cardiometabolic health: A systematic review of recent evidence

Cardiometabolic disease is a spectrum of diseases including, cardiovascular diseases, and metabolic syndrome. It is the leading cause of morbidity and mortality worldwide, with premature deaths being preventable. Currently, sleep has emerged as a potential target for cardiometabolic disease prevention. Several epidemiological studies have provided ample evidence that objectively measured short sleep duration increases the risk of cardiometabolic disease. However, the findings are inconsistent, and few studies measure sleep duration on cardiometabolic profiles objectively. Therefore, in this review, we focused on the recently published literature that explored the association between objectively measured sleep duration and cardiometabolic profiles (cardiovascular diseases, type 2 diabetes mellitus, and metabolic syndrome), seeking more insights regarding the applicability and, in turn, the impact of objectively measured sleep duration on cardiometabolic health, which is relatively understudied. We retrieved the information manually from PubMed, Google Scholar, HINARI, and the Cochrane Library from 2015 to 2022 using appropriate search terms, we included 49 articles. In this review, we found a strong relationship between objectively measured sleep duration and the risk of cardiometabolic disease, indicating that objectively measured short sleep durations increase cardiometabolic risks. In general, the association between objectively measured sleep duration and increased cardiometabolic risks (CMR) has been well-documented in higher-income countries. Several studies found that longer sleep duration was associated with a more favorable cardiometabolic profile in early adolescence, independent of other risk factors. On the other hand, objectively measured short sleep duration is associated with adverse cardiometabolic health outcomes such as coronary heart disease, hypertension, type 2 diabetes mellitus, and metabolic syndrome.
Enhanced sweet taste perception in obesity: Joint analysis of gustatory data from multiple studies

Introduction While sweet taste perception is a potential determinant of feeding behavior in obesity, the supporting evidence is inconsistent and is typically associated with methodological limitations. Notably, possible associations between sweet taste perception and measures of food reward remain undetermined. Materials and methods We conducted a cross-sectional analysis comparing 246 individuals with severe obesity and 174 healthy volunteers using a validated method for taste perception assessment. We included gustatory variables, namely intensity and pleasantness ratings of sour, salt, sweet, and bitter tastants, and taste thresholds assessed by electrogustometry. Reward-related feeding behavior, including hedonic hunger, food addiction, feeding behavior traits, and acceptance of foods and alcohol, was evaluated using self-rated scales for comparison with gustatory measures. Result In logistic regressions adjusted for age, gender, educational level, and research center, we found that a greater likelihood of belonging to the obesity group was associated with higher sweet intensity ratings (OR = 1.4, P = 0.01), hedonic hunger, food addiction symptoms, restrained and emotional eating (1.7 < OR ≤ 4.6, all P ≤ 0.001), and lower alcohol acceptance (OR = 0.6, P = 0.0002). Using principal component analysis, we found that while hedonic hunger, food addiction, and emotional eating were strongly interrelated, they were not associated with sweet intensity perception that, in turn, had a closer relationship with alcohol acceptance and restrained eating. Conclusion We found that individuals with obesity report higher sweet taste intensity ratings than healthy controls. Furthermore, while psychological measures of reward-related feeding behavior assess a common construct, sweet intensity perception may represent a different obesity-related dimension.
Effect of time-varying adherence to non-pharmaceutical interventions on the occurrence of multiple epidemic waves: A modeling study

Non-pharmaceutical interventions (NPIs) play a central role in infectious disease outbreak response and control. Their usefulness cannot be overstated, especially during the early phases of a new epidemic when vaccines and effective treatments are not available yet. These interventions can be very effective in curtailing the spread of infectious diseases when adequately implemented and sufficiently adopted by the public. However, NPIs can be very disruptive, and the socioeconomic and cultural hardships that come with their implementation interfere with both the ability and willingness of affected populations to adopt such interventions. This can lead to reduced and unsteady adherence to NPIs, making disease control more challenging to achieve. Deciphering this complex interaction between disease dynamics, NPI stringency, and NPI adoption would play a critical role in informing disease control strategies. In this work, we formulate a general-purpose model that integrates government-imposed control measures and public adherence into a deterministic compartmental epidemic model and study its properties. By combining imitation dynamics and the health belief model to encode the unsteady nature of NPI adherence, we investigate how temporal variations in NPI adherence levels affect the dynamics and control of infectious diseases. Among the results, we note the occurrence of multiple epidemic waves as a result of temporal variations in NPI adherence and a trade-off between the stringency of control measures and adherence. Additionally, our results suggest that interventions that aim at increasing public adherence to NPIs are more beneficial than implementing more stringent measures. Our findings highlight the necessity of taking the socioeconomic and cultural realities of affected populations into account when devising public health interventions.