Miracle-dz/UniFAR-FTUs huggingface dataset:
Risks to Bangladeshi children and young people during covid-19 outbreak

Children and young people are at higher risk of adverse health outcomes including obesity, neglect, and abuse by parents, and thus are more prone to mental health and chronic health issues during the covid-19 pandemic.1 The current lockdown situation has led children and young people into a sedentary lifestyle, which might increase the incidence of obesity and …
Differences in antibody kinetics and functionality between severe and mild SARS-CoV-2 infections.

We determined and compared the humoral immune response in severe, hospitalized and mild, non-hospitalized COVID-19 patients. Severe patients (n=38) develop a robust antibody response to SARS-CoV-2, including IgG and IgA antibodies. The geometric mean 50% virus neutralization titer is 1:240. SARS-CoV-2 infected hospital personnel (n=24), who developed mild symptoms necessitating leave of absence, self-isolation, but not hospitalization, 75 % develop antibodies, but with low/absent virus neutralization (60% < 1:20). While severe COVID-19 patients develop a strong antibody response, mild SARS-CoV-2 infections induce a modest antibody response. Long term monitoring will show whether these responses predict protection against future infections.
The Affect Heuristic and Risk Perception – Stability Across Elicitation Methods and Individual Cognitive Abilities

The reliance on feelings when judging risks and benefits is one of the most fundamental valuation processes in risk perception. Although previous research suggests that the affect heuristic reliably predicts an inverse correlation between risk and benefit judgments, it has not yet been tested if the affect heuristic is sensitive to elicitation method effects (joint/separate evaluation) and to what extent individual differences in cognitive abilities may mediate the risk–benefit correlation. Across two studies we find that (1) the risk–benefit correlation is stable across different elicitation methods and for different domains (e.g., social domain, sensation-seeking domain, health domain, economic domain) and (2) the strength of the inverse correlation is tied to individual cognitive abilities—primarily cognitive reflection ability.
Wesley J. Thompson (1947–2019)

Wesley Jay (Wes) Thompson, known for his seminal contributions to the studies of neuromuscular synapses and glial cells, passed away on March 26, 2019, at the age of 71. Surviving family, friends, trainees, colleagues, and others who hold Wes in fond memory gathered at the campus of Texas A&M University in College Station, TX, for a memorial service in his honor some weeks later. All of the speakers recounted with fondness their interactions withWes that exemplified the impact his approaches to life and work had on the lives of many others. Most who knewWes would agree that he never liked to talk about himself much except in performing self-deprecating humor and voicing his displeasure with the current political climate. Wes abhorred any public recognition of his accomplishments (or good deeds) and only begrudgingly tolerated those accompanied by research funds.We are certainWes would have protested the production of this article that pays him tribute. There are aspects of his life, however, that were readily apparent to those who have spent any meaningful amount of time with Wes—his generosity, drive and integrity as a scientist, affinity for history and simpler things in life such as his vegetable garden and backyard barbeque with friends that brought him joy, and love for his daughter. Jeff Lichtman, a fellow towering contemporary figure in the study of neuromuscular synapses, once called Wes “a gem of a human being,” a much-deserved compliment that sweetly sums up the warm character of the man we all knew. Wes was born on December 10, 1947, in Alice, TX, to Jay and Harriett Thompson from whom Wes inherited many of his endearing traits: rough-around-the-edges Texan charm, a desire/willingness to fix any mechanical instruments (often with mixed results) from his inventor/oilman father, and a deep belief in the value of education from his mother, a school teacher. The family relocated to Crawford, TX, where Wes spent a large part of his childhood. In 1966, Wes graduated from Crawford High School, and in 1970, earned a Bachelor of Science degree with High Honors from North Texas State University (since renamed the University of North Texas), a short 2-h drive away. Perhaps as a preview of what lay ahead in his long and respected scientific career punctuated with honors, a survey of his high school and college yearbooks (Figure 1) provides hints that fellow students had already recognized Wes’s academic accomplishments and potential. As his career progressed, clearly,Wes did not fall victim to enemies of promise. In addition to receiving several postdoctoral fellowships, he was named a Searle Scholar in 1981 (Searle Scholars Program, Chicago Community Trust) and received a National Institutes of Health (NIH) Research Career Development Award in 1984 and a Javits Neuroscience Investigator award in 2001. He maintained a research program that was continuously funded by the NIH until his death. As a testament to his long-standing dedication to educate undergraduate students, Wes was also recognized with a University of Texas at Austin Teaching Excellence Award in 2000.
Molecular Analysis of S-morphology Aflatoxin Producers From the United States Reveals Previously Unknown Diversity and Two New Taxa

Aflatoxins are highly toxic carcinogens that detrimentally influence profitability of agriculture and the health of humans and domestic animals. Several phylogenetically distinct fungi within Aspergillus section Flavi have S-morphology (average sclerotial size < 400 μm), and consistently produce high concentrations of aflatoxins in crops. S-morphology fungi have been implicated as important etiologic agents of aflatoxin contamination in the United States (US), but little is known about the diversity of these fungi. The current study characterized S-morphology fungi (n = 494) collected between 2002 and 2017, from soil and maize samples, in US regions where aflatoxin contamination is a perennial problem. Phylogenetic analyses based on sequences of the calmodulin (1.9 kb) and nitrate reductase (2.1 kb) genes resolved S-morphology isolates from the US into four distinct clades: (1) Aspergillus flavus S-morphotype (89.7%); (2) Aspergillus agricola sp. nov. (2.4%); (3) Aspergillus texensis (2.2%); and (4) Aspergillus toxicus sp. nov. (5.7%). All four S-morphology species produced high concentrations of aflatoxins in maize at 25, 30, and 35°C, but only the A. flavus S-morphotype produced unacceptable aflatoxin concentrations at 40°C. Genetic typing of A. flavus S isolates using 17 simple sequence repeat markers revealed high genetic diversity, with 202 haplotypes from 443 isolates. Knowledge of the occurrence of distinct species and haplotypes of S-morphology fungi that are highly aflatoxigenic under a range of environmental conditions may provide insights into the etiology, epidemiology, and management of aflatoxin contamination in North America.
Overexpression of the NMig1 Gene Encoding a NudC Domain Protein Enhances Root Growth and Abiotic Stress Tolerance in Arabidopsis thaliana

The family of NudC proteins has representatives in all eukaryotes and plays essential evolutionarily conserved roles in many aspects of organismal development and stress response, including nuclear migration, cell division, folding and stabilization of other proteins. This study investigates an undescribed Arabidopsis homolog of the Aspergillus nidulans NudC gene, named NMig1 (for Nuclear Migration 1), which shares high sequence similarity to other plant and mammalian NudC-like genes. Expression of NMig1 was highly upregulated in response to several abiotic stress factors, such as heat shock, drought and high salinity. Constitutive overexpression of NMig1 led to enhanced root growth and lateral root development under optimal and stress conditions. Exposure to abiotic stress resulted in relatively weaker inhibition of root length and branching in NMig1-overexpressing plants, compared to the wild-type Col-0. The expression level of antioxidant enzyme-encoding genes and other stress-associated genes was considerably induced in the transgenic plants. The increased expression of the major antioxidant enzymes and greater antioxidant potential correlated well with the lower levels of reactive oxygen species (ROS) and lower lipid peroxidation. In addition, the overexpression of NMig1 was associated with strong upregulation of genes encoding heat shock proteins and abiotic stress-associated genes. Therefore, our data demonstrate that the NudC homolog NMig1 could be considered as a potentially important target gene for further use, including breeding more resilient crops with improved root architecture under abiotic stress.
Endogenous and Exogenous Vanilloids Evoke Disparate TRPV1 Activation to Produce Distinct Neuronal Responses

Neuronal signals are processed along the nociceptive pathway to convey discriminative information, which would manifest in the produced pain sensation. The transient receptor potential vanilloid 1 (TRPV1), an important signaling complex in nociceptors termini, is activated by different noxious stimuli that underlie distinct pain sensations. For example, while endovanilloids are associated with inflammatory pain and hypersensitivity through TRPV1 activation, the exovanilloid toxin, capsaicin, evokes an acute pain by activating this channel. Differences in the TRPV1 activation profile evoked by exogenous and endogenous vanilloids were suggested to underlie this disparity in pain sensations. However, the cellular processes that lead to these differences in pain sensation mediated by the same channel are not fully understood. Here, we sought to describe the neuronal response of TRPV1-expressing nociceptors to exo-and endovanilloids. To this end, we performed current-clamp recordings in rat trigeminal neurons exposed to either capsaicin or intracellular endovanilloids produced downstream of the bradykinin receptor BK2. Our results show that lipoxygenase metabolites generate persistent TRPV1-dependent action potential firing while capsaicin evokes robust depolarization and high-frequency firing that is quickly terminated by depolarization block. Additionally, we found that a weak TRPV1 activation prolongs action potential firing. Overall, our results indicate different firing patterns evoked by inflammatory mediators and capsaicin via TRPV1 that correlate with the respective subsequent pain sensation. These findings also suggest that differences in neuronal activation stem from the variable degree of TRPV1 activation they produce.
Neuroscience in Context: The New Flagship of the Cognitive Sciences

Cognitive neuroscience has come to be viewed as the flagship of the cognitive sciences and is transforming our understanding of the nature of mind. In this paper we survey several research fields in cognitive neuroscience (lateralization, neuroeconomics, and cognitive control) and note that they are making rapid progress on fundamental issues. Lateralization research is developing a comparative framework for evolutionary analysis, and is identifying individual- and population-level factors that favor brain asymmetries. Neuroeconomics is creating a research framework for studying valuation mechanisms in the brain that allows investigation of more complex phenomena than traditional reward paradigms. And cognitive control research has cast light on the mechanisms of task learning and top-down control that enable fluid goal-directed behavior in complex situations. Reasons for the success of cognitive neuroscience are varied: technical advances (e.g., in molecular genetics and imaging) are part of the story, but the more important factors have to do with the power of integrating a bottom-up mechanistic approach with larger functional and evolutionary perspectives. The incorporation of a bottom-up component differentiates cognitive neuroscience from the multidisciplinary ambitions of classical cognitive science, which suffered from having limited access to mechanisms.
Insights into eukaryogenesis from the fossil record

Eukaryogenesis—the process by which the eukaryotic cell emerged—has long puzzled scientists. It has been assumed that the fossil record has little to say about this process, in part because important characters such as the nucleus and mitochondria are rarely preserved, and in part because the prevailing model of early eukaryotes implies that eukaryogenesis occurred before the appearance of the first eukaryotes recognized in the fossil record. Here, I propose a different scenario for early eukaryote evolution than is widely assumed. Rather than crown group eukaryotes originating in the late Paleoproterozoic and remaining ecologically minor components for more than half a billion years in a prokaryote-dominated world, I argue for a late Mesoproterozoic origin of the eukaryotic crown group, implying that eukaryogenesis can be studied using the fossil record. I review the proxy records of four crown group characters: the capacity to form cysts as evidenced by the presence of excystment structures; a complex cytoskeleton as evidenced by spines or pylomes; sterol synthesis as evidenced by steranes; and aerobic respiration—and therefore mitochondria—as evidenced by eukaryotes living in oxic environments, and argue that it might be possible to use these proxy records to infer the order in which these characters evolved. The records indicate that both cyst formation and a complex cytoskeleton appeared by late Paleoproterozoic time, and sterol synthesis appeared in the late Mesoproterozioc or early Neoproterozoic. The origin of aerobic respiration cannot as easily be pinned down, but current evidence permits the possibility that it evolved sometime in the Mesoproterozoic.
Sequence Memory in the Hippocampal–Entorhinal Region

Abstract Episodic memories are constructed from sequences of events. When recalling such a memory, we not only recall individual events, but we also retrieve information about how the sequence of events unfolded. Here, we focus on the role of the hippocampal–entorhinal region in processing and remembering sequences of events, which are thought to be stored in relational networks. We summarize evidence that temporal relations are a central organizational principle for memories in the hippocampus. Importantly, we incorporate novel insights from recent studies about the role of the adjacent entorhinal cortex in sequence memory. In rodents, the lateral entorhinal subregion carries temporal information during ongoing behavior. The human homologue is recruited during memory recall where its representations reflect the temporal relationships between events encountered in a sequence. We further introduce the idea that the hippocampal–entorhinal region might enable temporal scaling of sequence representations. Flexible changes of sequence progression speed could underlie the traversal of episodic memories and mental simulations at different paces. In conclusion, we describe how the entorhinal cortex and hippocampus contribute to remembering event sequences—a core component of episodic memory.
Randomized, Double-Blind Assessment of LFP Versus SUA Guidance in STN-DBS Lead Implantation: A Pilot Study

Background: The efficacy of deep brain stimulation (DBS) therapy in Parkinson’s disease (PD) patients is highly dependent on the precise localization of the target structures such as subthalamic nucleus (STN). Most commonly, microelectrode single unit activity (SUA) recordings are performed to refine the target. This process is heavily experience based and can be technically challenging. Local field potentials (LFPs), representing the activity of a population of neurons, can be obtained from the same microelectrodes used for SUA recordings and allow flexible online processing with less computational complexity due to lower sampling rate requirements. Although LFPs have been shown to contain biomarkers capable of predicting patients’ symptoms and differentiating various structures, their use in the localization of the STN in the clinical practice is not prevalent. Methods: Here we present, for the first time, a randomized and double-blinded pilot study with intraoperative online LFP processing in which we compare the clinical benefit from SUA- versus LFP-based implantation. Ten PD patients referred for bilateral STN-DBS were randomly implanted using either SUA or LFP guided targeting in each hemisphere. Although both SUA and LFP were recorded for each STN, the electrophysiologist was blinded to one at a time. Three months postoperatively, the patients were evaluated by a neurologist blinded to the intraoperative recordings to assess the performance of each modality. While SUA-based decisions relied on the visual and auditory inspection of the raw traces, LFP-based decisions were given through an online signal processing and machine learning pipeline. Results: We found a dramatic agreement between LFP- and SUA-based localization (16/20 STNs) providing adequate clinical improvement (51.8% decrease in 3-month contralateral motor assessment scores), with LFP-guided implantation resulting in greater average improvement in the discordant cases (74.9%, n = 3 STNs). The selected tracks were characterized by higher activity in beta (11–32 Hz) and high-frequency (200–400 Hz) bands (p < 0.01) of LFPs and stronger non-linear coupling between these bands (p < 0.05). Conclusion: Our pilot study shows equal or better clinical benefit with LFP-based targeting. Given the robustness of the electrode interface and lower computational cost, more centers can utilize LFP as a strategic feedback modality intraoperatively, in conjunction to the SUA-guided targeting.
Nutrient Resorption Efficiency and Proficiency in Fronds and Trophopods of a Winter‐Deciduous Fern, Dennstaedtia punctilobula

We measured resorption of nitrogen (N), phosphorus (P), copper (Cu), manganese (Mn), and zinc (Zn) in the fronds and trophopods of 12 populations of a winter‐deciduous fern, Dennstaedtia punctilobula, because (1) resorption (the withdrawal of nutrients from senescing tissues) has been measured in only one other winter‐deciduous fern and one wintergreen fern, (2) the majority of what is known about resorption is derived from studies of woody plants, and (3) the architecture and life history of ferns differ fundamentally from woody perennials in ways that could significantly impact resorption. Resorption of N and P from fronds was both efficient (52% N, 50% P; percentage of nutrient in green leaves withdrawn during senescence) and proficient (0.7% N, 0.07% P; concentration of nutrient in fully senesced leaves). Copper was resorbed from fronds (17% resorption efficiency), but Mn and Zn were not. Rhizomes were a significant sink for resorbed N and P, but trophopods (enlargements of the petiole thought to function as storage organs) were neither a source nor a sink for resorbed nutrients. In comparisons with other herbaceous life‐forms of plants, resorption of N and P in ferns was similar in efficiency to that in graminoids and forbs but dissimilar in proficiency to that in forbs. Therefore, while these data support the tenet that resorption efficiency does not differ among life‐forms, they also reveal apparent fundamental differences in resorption proficiency among life‐form classes of plants.
The Diversity of Lipopolysaccharide (O) and Capsular Polysaccharide (K) Antigens of Invasive Klebsiella pneumoniae in a Multi-Country Collection

Klebsiella pneumoniae is a common cause of sepsis and is particularly associated with healthcare-associated infections. New strategies are needed to prevent or treat infections due to the emergence of multi-drug resistant K. pneumoniae. The goal of this study was to determine the diversity and distribution of O (lipopolysaccharide) and K (capsular polysaccharide) antigens on a large (>500) global collection of K. pneumoniae strains isolated from blood to inform vaccine development efforts. A total of 645 K. pneumoniae isolates were collected from the blood of patients in 13 countries during 2005–2017. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method. O antigen types including the presence of modified O galactan types were determined by PCR. K types were determined by multiplex PCR and wzi capsular typing. Sequence types of isolates were determined by multilocus sequence typing (MLST) targeting seven housekeeping genes. Among 591 isolates tested for antimicrobial resistance, we observed that 19.3% of isolates were non-susceptible to carbapenems and 62.1% of isolates were multidrug resistant (from as low as 16% in Sweden to 94% in Pakistan). Among 645 isolates, four serotypes, O1, O2, O3, and O5, accounted for 90.1% of K. pneumoniae strains. Serotype O1 was associated with multidrug resistance. Fifty percent of 199 tested O1 and O2 strains were gmlABC-positive, indicating the presence of the modified polysaccharide subunit D-galactan III. The most common K type was K2 by both multiplex PCR and wzi capsular typing. Of 39 strains tested by MLST, 36 strains were assigned to 26 known sequence types of which ST14, ST25, and ST258 were the most common. Given the limited number of O antigen types, diverse K antigen types and the high multidrug resistance, we believe that an O antigen-based vaccine would offer an excellent prophylactic strategy to prevent K. pneumoniae invasive infection.
Point of care lung ultrasound is useful when screening for CoVid-19 in Emergency Department patients

Background CoVid-19 can be a life-threatening lung disease or a trivial upper respiratory infection depending on whether the alveoli are involved. Emergency department (ED) screening in symptomatic patients with normal vital signs is frequently limited to oro-nasopharyngeal swabs. We tested the null hypothesis that patients being screened for CoVid-19 in the ED with normal vital signs and without hypoxia would have a point-of-care lung ultrasound (LUS) consistent with CoVid-19 less than 2% of the time. Methods Subjects Subjects were identified from ED ultrasound logs. Inclusion criteria Age 14 years or older with symptoms prompting ED screening for CoVid-19. Exclusion criteria Known congestive heart failure or other chronic lung condition likely to cause excessive B lines on LUS. Intervention Structured blinded ultrasound review and chart review Analysis We used an exact hypothesis tests for binomial random variables. We also measured LUS diagnostic performance using computed tomography as the gold standard. Results We reviewed 77 charts; 62 met inclusion criteria. Vital signs were normal in 31 patients; 10 (32%) of these patients had LUS consistent with CoVid-19. We rejected the null hypothesis (p-value for bitest <0.001). The treating physicians' interpretation of their own point of care lung ultrasounds had a sensitivity of 100% (95% CI 75%, 100%) and specificity of 80% (95% CI 68%, 89%). Conclusion LUS has a meaningful detection rate for CoVid-19 in symptomatic emergency department patients with normal vital signs. We recommend at least LUS be used in addition to PCR testing when screening symptomatic ED patients for CoVid-19.