Miracle-dz/UniFAR-FTUs huggingface dataset:
CHILD HEALTH BOOKLET: MONITORING GROWTH AND CHILD DEVELOPMENT CADERNETA DE SAÚDE DA CRIANÇA: VIGILÂNCIA DO CRESCIMENTO E DESENVOLVIMENTO INFANTIL LIBRETA DE SALUD DEL NIÑO: VIGILANCIA DEL CRECIMIENTO Y DESARROLLO INFANTIL

Objective: to analyze the scientific evidence regarding the use of the Child Health Booklet by health professionals for the monitoring of child growth and development. Method: a bibliographical study, type integrative, with searches in LILACS and BDENF databases and in the SCIELO virtual library, using Descriptors in Health Sciences; growth and development, primary health care and personal health records, which were cross-referenced with the child descriptor employing the Boolean operator and. 15 articles published between 2014 and 2018 were selected and the results are presented in figure form. Results: the poor use of CHB was evidenced in the studies, which is related to the absence and fragility of records, the difficulty of professionals to perceive the relevance of filling, poor knowledge of professionals, insufficient guidance to families, besides the participation of the family in this process. Conclusion: due to the precarious nature of the use of CHB, it is committed to monitoring child health as this is the essential instrument for monitoring child growth and development. Descriptors: Growth and Development; Primary Health Care; Personal Health Records; Kid; Comprehensive Health Care; Child Health.
OP-CVRE180368 1500..1511

Aims The human ether-a-go-go-related gene (hERG) encodes the rapidly activating delayed rectifier potassium channel (IKr). Malfunction of hERG/IKr is the primary cause of acquired long QT syndrome (LQTS), an electrical disorder of the heart that can cause arrhythmias and sudden death. Patients with autoimmune diseases display a high incidence of LQTS. While dysfunction of hERG channels induced by autoantibodies such as anti-Ro52 may play a role in this pathology, the underlying mechanisms are not well understood. Here, we investigated the acute and chronic effects of anti-Ro52 antibody on hERG channels stably expressed in human embryonic kidney (hERG-HEK) 293 cells as well as IKr in neonatal rat ventricular myocytes. Methods and results Using whole-cell patch clamp, western blot analyses, and immunocytochemistry, we found that a 12-h treatment of hERG-HEK cells with patients’ sera containing anti-Ro52 autoantibody decreased the hERG current (IhERG) by 32% compared to cells treated with autoantibody-negative patients’ sera. Commercial anti-Ro52 antibody at 100mg/mL did not acutely block IhERG. Instead, a 12-h treatment with anti-Ro52 antibody at a concentration of 4 mg/mL significantly reduced mature hERG protein expression and IhERG. Specifically, anti-Ro52 antibody did not acutely block hERG current but chronically facilitated hERG endocytic degradation. The extracellular S5-pore linker of hERG, which forms the turret of the channel on the outside of the cell, is the target region for anti-Ro52-mediated hERG reduction since its replacement with the analogous region of EAG abolished the anti-Ro52 effect. In neonatal rat ventricular myocytes, 100 mg/mL anti-Ro52 antibody did not acutely block IKr, but a 12-h treatment of cells with 4mg/mL anti-Ro52 antibody selectively reduced IKr and prolonged the action potential duration. Conclusions Our results indicate that anti-Ro52 antibody acts on the hERG S5-pore linker to chronically decrease hERG expression and current. These findings provide novel insights into hERG regulation and anti-Ro52 antibody-associated LQTS.
Functional importance and structure of small ribosomal protein RACK1

The Receptor for Activated C Kinase 1 (RACK1) protein is one of the ribosomal proteins that is located in the small subunit which is maintained across most of the eukaryotes and functions as a flexible scaffold protein involved in multiple signaling pathways. It is considered as a highly conserved protein that found in a wide range of tryptophan-aspartate (WD40) repeat proteins of eukaryotic organisms, from Chlamydomonas to plants and mammals. RACK1 adopts a propeller structure with seven blades structures, enabling the binding capability of the protein. RACK1 participates in shuttling and anchoring of proteins around the cells at certain positions and it stabilizes protein production during translation. RACK1 is a member of the protein family WD repeat that consists of seven-bladed βpropeller structure which shares important homology to the G-proteins (Gb) β-subunit. It interacts with the ribosomal machinery, multiple receptors of the cell surface and the nuclear proteins. RACK1 is a key mediator of different pathways and contributes to a variety of cellular functional aspects. This review discusses the main function of RACK1 protein in eukaryotes including animals and plants. Furthermore, we will show the recent study on RACK1 function in different organisms. © 2022 Production by the University of Garmian. This is an open access article under the LICENSE https://creativecommons.org/licenses/by-nc/4.0/
The Protective Role of E-64d in Hippocampal Excitotoxic Neuronal Injury Induced by Glutamate in HT22 Hippocampal Neuronal Cells

Epilepsy is the most common childhood neurologic disorder. Status epilepticus (SE), which refers to continuous epileptic seizures, occurs more frequently in children than in adults, and approximately 40–50% of all cases occur in children under 2 years of age. Conventional antiepileptic drugs currently used in clinical practice have a number of adverse side effects. Drug-resistant epilepsy (DRE) can progressively develop in children with persistent SE, necessitating the development of novel therapeutic drugs. During SE, the persistent activation of neurons leads to decreased glutamate clearance with corresponding glutamate accumulation in the synaptic extracellular space, increasing the chance of neuronal excitotoxicity. Our previous study demonstrated that after developmental seizures in rats, E-64d exerts a neuroprotective effect on the seizure-induced brain damage by modulating lipid metabolism enzymes, especially ApoE and ApoJ/clusterin. In this study, we investigated the impact and mechanisms of E-64d administration on neuronal excitotoxicity. To test our hypothesis that E-64d confers neuroprotective effects by regulating autophagy and mitochondrial pathway activity, we simulated neuronal excitotoxicity in vitro using an immortalized hippocampal neuron cell line (HT22). We found that E-64d improved cell viability while reducing oxidative stress and neuronal apoptosis. In addition, E-64d treatment regulated mitochondrial pathway activity and inhibited chaperone-mediated autophagy in HT22 cells. Our findings indicate that E-64d may alleviate glutamate-induced damage via regulation of mitochondrial fission and apoptosis, as well as inhibition of chaperone-mediated autophagy. Thus, E-64d may be a promising therapeutic treatment for hippocampal injury associated with SE.
A rare case of rickettsial fever with isolated third cranial nerve palsy causing hemophagocytic lymphohistiocytosis

Rickettsial infections are being increasingly diagnosed in the Indian subcontinent with the advent of affordable and sensitive diagnostic techniques. Rickettsial infections are sometimes complicated by the development of secondary hemophagocytic lymphohistiocytosis (HLH) which is life-threatening unless identified early and treated. Here, we describe a case of rickettsial fever in a 41-year-old male who presented with fever, skin rash, and neurological obtundation, complicated by both third cranial nerve palsy and HLH. The patient showed a good recovery in sensorium with intravenous doxycycline and dexamethasone, although the oculomotor nerve palsy persisted for about two months after discharge. This case highlights the need to initiate treatment for rickettsial fever and HLH on clinical grounds before waiting for confirmation by laboratory tests.
COPD_A_325925 2901..2910

1Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, People’s Republic of China; 2National Clinical Research Center for Respiratory Diseases, Beijing, People’s Republic of China; 3Department of Pulmonary and Critical Care Medicine, Quanzhou First Hospital Affiliated Fujian Medical University, Quanzhou, Fujian, People’s Republic of China; 4Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, People’s Republic of China; 5Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Purpose: To identify clinical features and outcomes associated with pulmonary heart disease among patients with chronic obstructive pulmonary disease exacerbation (COPD), which may help reduce economic burden accrued over hospital stay and shorten length of stay (LOS). Patients and Methods: Totally, 4386 patients with acute exacerbation of COPD (AECOPD) classified into pulmonary heart disease (PHD) group and non-pulmonary heart disease group, were included from the ACURE registry, a prospective multicenter patient registry study. Clinical features and outcomes were compared between groups. Results: PHD patients had a more severe profile, including having higher scores of COPD assessment test and modified British Medical Research Council, worse lung function, more patients hospitalized more than once in the past year due to acute exacerbation of COPD, and more comorbidities. Furthermore, drug cost was higher and length of stay was longer in AECOPD patients with PHD. Conclusion: AECOPD patients with PHD had a more severe profile and worse clinical outcomes, including higher drug cost and longer LOS. PHD is an independent risk factor of drug cost and LOS. Complicated with PHD in COPD/AECOPD patients with PHD means heavier disease burden and worse prognosis. It merits further study to focus on PHD management in COPD/AECOPD patients.
OP-IJEJ180270 1152..1160

Background: Little is understood about the causes of adolescent onset idiopathic scoliosis (AIS). No prospective studies assessing the association between physical activity and idiopathic adolescent scoliosis have been carried out. We aimed to carry out the first prospective population-based study of this association. Methods: The Avon Longitudinal Study of Parents and Children (ALSPAC) collected selfreported measures of physical ability/activity at ages 18 months and 10 years. Objective measures of physical activity were collected by accelerometry at age 11 years. scoliosis was identified using the dxa scoliosis Method at age 15 years. Participants with scoliosis at age 10 years were excluded. Results: Of 4640 participants at age 15 years who had DXA scans, 267 (5.8%) had scoliosis. At age 18 months, those infants who were able to stand up without being supported were 66% less likely to have developed scoliosis by age 15 (P1⁄4 0.030) compared with infants who could not. Those children whose mothers reported they did most vigorous physical activity at age 10 years were 53% less likely to develop scoliosis (P1⁄40.027). Those children who did more objectively measured moderate/vigorous physical activity at age 11 were 30% less likely to have developed scoliosis (P<0.001). Results were not affected by adjustment for age, gender, lean mass, fat mass or back pain. Conclusions: We report reduced physical ability and activity as early as age 18 months in those who go on to develop scoliosis by age 15 years. Further research is justified to examine the mechanisms underlying this association.
The Impact of COVID-19 on Eating Disorder Risks and Symptoms in the General Population: A Systematic Review

Aims: This study aims to identify the risk factors of eating disorders, evaluate the impact of the pandemic on those with or without pre-existing eating disorders and compare the impact of COVID-19 on the various eating disorders. Methodology: We searched Public/Publisher MEDLINE (PubMed) and Excerpta Medica dataBASE (EMBASE) databases from January 2019 to May 2021, 11 observational studies were selected out of 81 initially identified articles. The sample size ranged from 15 to 22,374 participants, with a total of 31,404 participants. Results: Individuals, especially females aged 18-25 years and 30-45 years were shown to be more susceptible to eating disorder risks and symptoms. Depression and anxiety were shown to be the most prevalent risk factors among all subjects. Exacerbation of symptoms was observed in subjects with the eating disorders except for the anorexia nervosa subgroup which showed minimal changes. Conclusion: This study established the fact that COVID-19 pandemic had a negative impact on the eating habits of people with or without pre-existing eating disorders (ED). This study also highlights the need for efforts to specifically identify evidence-based measures to support patients with ED during and after a pandemic.
Influence of Clay Fraction and Mineral Composition on Unsaturated Characteristics of Cohesive Foundation Soil in Airports

Taking unsaturated clay foundation soil of an airport project in Hefei as the research object, the effects of particle gradation and mineral composition on the unsaturated soil properties were analyzed through two kinds of tests. The results show that there is a good correlation between the residual water content and the clay fraction or silt fraction content in the grading, and the residual water content has a significant positive linear correlation with the clay fraction content, but a negative linear correlation with the silt fraction content. Residual matric suction has a nonlinear correlation with clay fraction or silt fraction content in gradation, which has a significant nonlinear negative correlation with clay fraction content and a positive nonlinear correlation with silt fraction content. The residual water content and the residual matric suction have obvious linear relationship with the content of montmorillonite but have no obvious correlation with the content of illite. The water-storage coefficient of unsaturated airfield foundation soil decreases exponentially with the increase of clay content and montmorillonite content.
Extracts of Persea americana Mill. that delay ripening in avocado fruits

The majority of postharvest research on the fruits of Persea americana Mill. is focused on delaying maturation. In this work, we studied the effect of the application of non-polar extracts obtained from branches, peduncle and avocado leaves ‘Hass’ variety on the delay of fruit ripening with and without peduncle. In addition, the maturation of P. americana fruits collected with their branches of original size with leaves (ROH) and without leaves (ROSH), and branches at half of their original size with leaves (RMH) and without leaves (RMSH) was evaluated. The variables measured in fruits treated with non-polar extracts were firmness, respiration, equatorial diameter and color. In the fruits with branches, firmness, equatorial diameter, length and color were determined. The firmness was determined with a hedonic scale of 5 to 1, the breathing speed (mL CO2 kg h) with a CO2 meter, the color variables (L, a, b) with the android application ‘Color Grab’, the equatorial diameter and length, were measured with a digital vernier. According to the results, the presence of the peduncle in fruits treated with hexane extracts delayed their maturation; with an average firmness of 4.3 and green coloration at 10 d post-harvest. The peduncle extracts at 100 and 200 ppm had a significant effect in maintaining the green coloration of the fruits until 10 d after harvest. The evaluation of maturity in fruits collected with branches, showed that the presence of leaves retards its maturation.
Social Network Community Detection by Combining Self-Organizing Maps and Genetic Algorithms

Social networks have become an important source of information from which we can extract valuable indicators that can be used in many fields such as marketing, statistics, and advertising among others. To this end, many research works in the literature offer users some tools that can help them take advantage of this mine of information. Community detection is one of these tools and aims to detect a set of entities that share some features within a social network. We have taken part in this effort, and we proposed an approach mainly based on pattern recognition techniques. The novelty of this approach is that we do not directly tackle the social networks to find these communities. We rather proceeded in two stages; first, we detected community cores through a special type of self-organizing map called the Growing Hierarchical Self-Organizing Map (GHSOM). In the second stage, the agglomerations resulting from GHSOM were grouped to retrieve the final communities. The quality of the final partition would be under the control of an evaluation function that is maximized through genetic algorithms. Our system was tested on real and artificial databases, and the obtained results are really encouraging.
Association of Age at First Severe Respiratory Syncytial Virus Disease With Subsequent Risk of Severe Asthma: A Population-Based Cohort Study

Nusrat Homaira,1,4, Nancy Briggs,2 Ju-Lee Oei,1,5 Lisa Hilder,1,3 Barbara Bajuk,6 Adam Jaffe,1,4 and Saad B. Omer7 1Discipline of Paediatrics, School of Women’s and Children’s Health, UNSW Medicine, 2Stats Central, Mark Wainwright Analytical Centre, and 3Centre for Big Data Research in Health, University of New South Wales, Sydney, and 4Respiratory Department, Sydney Children’s Hospital, 5Department of Newborn Care, Royal Hospital for Women, and 6New South Wales Pregnancy and Newborn Services Network, Sydney Children’s Hospitals Network, Randwick, Australia; and 7Schools of Public Health and Medicine, Emory University, Atlanta, Georgia
Performance and microbiota of the digestive tract of Nellore calves supplemented with fungi isolated from bovine rumen

Abstract Background and Aim: In tropical semiarid regions, supplementation with fungi could contribute to rumen modulation, promoting greater production of fibrolytic enzymes and degradation of forage. The objective of this study was to analyze the effect of supplementation with fungi, isolated from the bovine rumen, on the performance and microbiota of the digestive tract of Nellore calves. Materials and Methods: The experiment was conducted in randomized blocks evaluating eight Nellore calves that were daily supplemented with isolates of Aspergillus terreus and Trichoderma longibrachiatum, along with eight calves that were not supplemented. After 55 days, the animals were weighed, and samples of rumen fluid and feces were collected for analysis. The characteristics that showed normal distribution were subjected to analysis of variance and compared using Tukey’s test. Whereas, the variables that did not show normal distribution were subjected to the Kruskal–Wallis test, and the frequencies of the bacterial and fungal genera were compared using the Chi-square test. Results: Supplementation with fungi promoted the reduction in ruminal pH (p<0.05). However, the final live weight; average daily weight gain; total weight gain; rumen protozoa; and the count of Enterobacteriaceae, mycelial fungi, and yeasts of ruminal fluid and feces were not influenced by supplementation (p>0.05). Moreover, the protozoa Eodinium spp. was identified only in supplemented calves (p<0.05). Conclusion: Supplementation with the fungi presented the potential for use as possible additives because it did not alter the physiological parameters of the facultative anaerobic microbiota composition in the rumen and feces. In addition, it favored the presence of the ciliate genus Eodinium. However, further studies should be performed to better define suitable dosages for supplementation.
DDDT_A_333383 4403..4411

1The Second Peoples Hospital of Wuhu, Wuhu, 241001, Anhui, People’s Republic of China; 2School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand; 3Faculty of Thai Traditional Medicine, Prince of Songkla University, Hat Yai, 90110, Thailand Background: Over the past few decades, cyclophosphamide (CP) has been extensively used as a broad-spectrum alkylating agent for the treatment of various cancers and solid tumors. However, the therapeutic actions on CP are not limited to only cancer cells, as it simultaneously exerts significant toxicities on healthy cells through the instigation of oxidative stress and oxidative damages. CP induced testicular toxicity is associated with impaired spermatogenesis, reduced sperm functionality, reproductive hormone and testicular weight. This study was aimed at unravelling the protective effects of emodin (EMD) on testicular toxicity following CP treatment. Methods: Twenty-four male Wistar rats were allotted into 4 groups as normal control group (NCG), CP control group (CPCG), EMD25+CP (25 mg/kg in 5% tween 80) and EMD50+CP groups (50 mg/kg in 5% tween 80). EMD was orally administered for 35 consecutive days, while four doses of CP (100 mg/kg/week) were administered intraperitoneally from the second to fifth week of treatment. Thereafter, the animals were sacrificed and histopathological examination of the testes as well as serum/testicular biochemical assays were conducted. Results: The results revealed that CP significantly impeded sperm function parameters including sperm count, viability and motility as well as decreased reproductive hormones (testosterone, LH and FSH) levels. In addition, CP enhanced testicular oxidative stress and proinflammatory markers (MDA, IL-6 and TNF-α), while simultaneously decreasing testicular antioxidant enzymes (GSH, GPx, SOD and CAT). Evidence of marked histopathological alterations was also observed in the H&E stained testicular tissues of CP treated rats. EMD significantly prevented these CP induced negative effects. Conclusion: This study provides a basis for the potential use of EMD in counteracting chemotherapy induced testicular toxicity. The results further suggest that EMD testicular protective effects in CP-treated rats may be mediated through its modulatory role on oxidative stress and inflammation.
POPULATION GROWTH AND AUTOMATION DENSITY: THEORY AND CROSS-COUNTRY EVIDENCE

We analyse the effects of declining population growth on automation. Theoretical considerations imply that countries with lower population growth introduce automation technologies faster than those with higher population growth. We test the theoretical implication on panel data for 60 countries over the time span 1993-2013. Regression estimates support the theoretical implication, suggesting that a one percent increase in population growth is associated with an approximately two percent reduction in the growth rate of robot density. Our results are robust to the inclusion of standard control variables, different estimation methods, dynamic specifications, and changes with respect measuring robot stocks.