Miracle-dz/UniFAR-FTUs huggingface dataset:
Possible Effects of Social Media Use on Adolescent Health Behaviors and Perceptions

Social media use is one of the most popular leisure activities among adolescents. Concomitant to this is a growing concern regarding problematic social media use and its relationship with health behaviors. To further increase the body of research into this phenomenon, our study explored the relationship between problematic social media use and physical activity levels, sleep peculiarities, and life satisfaction in Lithuanian children and adolescents. The study was conducted in April to June 2018 during the Health Behaviour in School-aged Children survey in Lithuania, a collaborative project of the World Health Organization. The sample comprised 4,191 school children (mean age 13.9 ± 1.69 years). The findings show that problematic social media use associates with about two times higher odds for worse sleep quality and lower life satisfaction. Problematic social media use was related to lower levels of vigorous physical activity in girls, but the possible effect on moderate physical activity was in boys and was inverse—boys with problematic social media use reported higher levels of moderate physical activity. Overall, in our study, girls with problematic social media use tended to have more negative health perceptions than boys. The results suggest that problematic social media use is an independent risk factor for negative health behaviors. This study adds to the accumulating knowledge that problematic social media use among adolescents may lead to worse health perceptions and, likely, further negative health outcomes.
Biochemical mechanism and biological effects of the inhibition of silent information regulator 1 (SIRT1) by EX-527 (SEN0014196 or selisistat)

Abstract The human sirtuin silent information regulator 1 (SIRT1) is a NAD+-dependent deacetylase enzyme. It deacetylates many protein substrates, including histones and transcription factors, thereby controlling many physiological and pathological processes. Several synthetic inhibitors and activators of SIRT1 have been developed, and some therapeutic applications have been explored. The indole EX-527 and its derivatives are among the most potent and selective SIRT1 inhibitors. EX-527 has been often used as a pharmacological tool to explore the effect of SIRT1 inhibition in various cell types. Its therapeutic potential has, therefore, been evaluated in animal models for several pathologies, including cancer. It has also been tested in phase II clinical trial for the treatment of Huntington’s disease (HD). In this review, we will provide an overview of the literature on EX-527, including its mechanism of inhibition and biological studies.
Interleukin-6 Treatment Results in GLUT4 Translocation and AMPK Phosphorylation in Neuronal SH-SY5Y Cells

Interleukin-6 (IL-6) is a pleiotropic cytokine that can be released from the brain during prolonged exercise. In peripheral tissues, exercise induced IL-6 can result in GLUT4 translocation and increased glucose uptake through AMPK activation. GLUT4 is expressed in the brain and can be recruited to axonal plasma membranes with neuronal activity through AMPK activation. The aim of this study is to examine if IL-6 treatment: (1) results in AMPK activation in neuronal cells, (2) increases the activation of proteins involved in GLUT4 translocation, and (3) increases neuronal glucose uptake. Retinoic acid was used to differentiate SH-SY5Y neuronal cells. Treatment with 100 nM of insulin increased the phosphorylation of Akt and AS160 (p < 0.05). Treatment with 20 ng/mL of IL-6 resulted in the phosphorylation of STAT3 at Tyr705 (p ≤ 0.05) as well as AS160 (p < 0.05). Fluorescent Glut4GFP imaging revealed treatment with 20ng/mL of IL-6 resulted in a significant mobilization towards the plasma membrane after 5 min until 30 min. There was no difference in GLUT4 mobilization between the insulin and IL-6 treated groups. Importantly, IL-6 treatment increased glucose uptake. Our findings demonstrate that IL-6 and insulin can phosphorylate AS160 via different signaling pathways (AMPK and PI3K/Akt, respectively) and promote GLUT4 translocation towards the neuronal plasma membrane, resulting in increased neuronal glucose uptake in SH-SY5Y cells.
Overexpression of NEIL3 associated with altered genome and poor survival in selected types of human cancer

Base excision repair, which is initiated by the DNA N-glycosylase proteins, is the frontline for repairing potentially mutagenic DNA base damage. Several base excision repair genes are deregulated in cancer and affect cellular outcomes to chemotherapy and carcinogenesis. Endonuclease VIII-like 3 (NEIL3) is a DNA glycosylase protein that is involved in oxidative and interstrand crosslink DNA damage repair. Our previous work has showed that NEIL3 is required to maintain replication fork integrity. It is unknown whether NEIL3 overexpression could contribute to cancer phenotypes, and its prognostic value and use as potential drug target remain unexplored. Our analysis of cancer genomics data sets reveals that NEIL3 frequently undergoes overexpression in several cancers. Furthermore, patients who exhibited NEIL3 overexpression with pancreatic adenocarcinoma, lung adenocarcinoma, lower grade glioma, kidney renal clear cell carcinoma, and kidney papillary cell carcinoma had worse overall survival. Importantly, NEIL3 overexpressed tumors accumulate mutation and chromosomal variations. Furthermore, NEIL3 overexpressed tumors exhibit simultaneous overexpression of homologous recombination genes (BRCA1/2) and mismatch repair genes (MSH2/MSH6). However, NEIL3 overexpression is negatively correlated with tumor overexpressing nucleotide excision repair genes (XPA, XPC, ERCC1/2). Our results suggest that NEIL3 might be a potential prognosis marker for high-risk patients, and/or an attractive therapeutic target for selected cancers.
Increase in the concentrations of amino acids in the vascular tissue of white clover and white lupin after defoliation: an indication of a N feedback regulation of symbiotic N$_2$ fixation

La defoliation subie plus particulierement par les legumineuses fourrageres pâturees ou fauchees, est suivie par une decroissance rapide et spectaculaire de l'activite de la nitrogenase (reduction de C 2 H 2 , evolution de H 2 dans Ar/O 2 ). Puisque la demande d'azote de la plante est temporairement reduite apres la defoliation, l'activite de la nitogenase devrait etre abaissee grâce a un mecanisme de retroaction de l'azote. Pour tester cette hypothese, la concentration de trois acides amines dans la xyleme du trefle blanc et dans le phloeme du lupin blanc a ete mesuree avant et apres la defoliation. La concentration de l'asparagine de la glutamine et de l'aspartate libres etait la plus elevee de toutes celles des acides amines avant la defoliation. Elles ont triple dans le xyleme du trefle blanc et double dans le phloeme du lupin blanc dans une periode comprise entre une et trois heures apres la defoliation. Ces resultats sont en bon accord avec le concept d'un mecanisme de retroaction de l'azote qui regule la fixation de l'azote dans les legumineuses, dans lequel les acides amines libres, circulant dans le tissu vasculaire, agissent comme des molecules de declenchement.
Polymorphisms in human immunoglobulin heavy chain variable genes and their upstream regions

Abstract Germline variations in immunoglobulin genes influence the repertoire of B cell receptors and antibodies, and such polymorphisms may impact disease susceptibility. However, the knowledge of the genomic variation of the immunoglobulin loci is scarce. Here, we report 25 potential novel germline IGHV alleles as inferred from rearranged naïve B cell cDNA repertoires of 98 individuals. Thirteen novel alleles were selected for validation, out of which ten were successfully confirmed by targeted amplification and Sanger sequencing of non-B cell DNA. Moreover, we detected a high degree of variability upstream of the V-REGION in the 5′UTR, L-PART1 and L-PART2 sequences, and found that identical V-REGION alleles can differ in upstream sequences. Thus, we have identified a large genetic variation not only in the V-REGION but also in the upstream sequences of IGHV genes. Our findings provide a new perspective for annotating immunoglobulin repertoire sequencing data.
Ecology of the Mediterranean Gecko, Hemidactylus turcicus (Linnaeus, 1758) (Reptilia: Gekkonidae), in North Sinai, Egypt

Abstract Aspects of the ecology of Hemidactylus turcicus were studied on the campus of Suez Canal University at Al-Arish, north Sinai, Egypt, from January 2001 to December 2002. Lizards were very often encountered on fences and building walls and were commonly observed in horizontal and vertical positions. Lizards were active from March to November and on warm nights in winter; they started their activity after complete darkness and displayed a unimodal pattern. Their activity differed between the seasons. Lepidoptera were the most important food taken by lizards in terms of volume, frequency of occurrence and the index of relative importance. The highest number of food categories was recorded in spring and the lowest in winter. Food types differed according to locality. The reproductive season extended from March to August. The testes of adult males were enlarged from March to July with maximum size and mass during May and minimum during August.
Calcium-Dependent Hydrogen Peroxide Mediates Hydrogen-Rich Water-Reduced Cadmium Uptake in Plant Roots1[OPEN]

Root cadmium uptake is influenced by hydrogen via modulation of a plasma membrane-based respiratory burst oxidase homolog that operates upstream of a cadmium transporter protein. Hydrogen gas (H2) has a possible signaling role in many developmental and adaptive plant responses, including mitigating the harmful effects of cadmium (Cd) uptake from soil. We used electrophysiological and molecular approaches to understand how H2 ameliorates Cd toxicity in pak choi (Brassica campestris ssp. chinensis). Exposure of pak choi roots to Cd resulted in a rapid increase in the intracellular H2 production. Exogenous application of hydrogen-rich water (HRW) resulted in a Cd-tolerant phenotype, with reduced net Cd uptake and accumulation. We showed that this is dependent upon the transport of calcium ions (Ca2+) across the plasma membrane and apoplastic generation of hydrogen peroxide (H2O2) by respiratory burst oxidase homolog (BcRbohD). The reduction in root Cd uptake was associated with the application of exogenous HRW or H2O2. This reduction was abolished in the iron-regulated transporter1 (Atirt1) mutant of Arabidopsis (Arabidopsis thaliana), and pak choi pretreated with HRW showed decreased BcIRT1 transcript levels. Roots exposed to HRW had rapid Ca2+ influx, and Cd-induced Ca2+ leakage was alleviated. Two Ca2+ channel blockers, gadolinium ion (Gd3+) and lanthanum ion (La3+), eliminated the HRW-induced increase in BcRbohD expression, H2O2 production, and Cd2+ influx inhibition. Collectively, our results suggest that the Cd-protective effect of H2 in plants may be explained by its control of the plasma membrane-based NADPH oxidase encoded by RbohD, which operates upstream of IRT1 and regulates root Cd uptake at both the transcriptional and functional levels. These findings provide a mechanistic explanation for the alleviatory role of H2 in Cd accumulation and toxicity in plants.
Patterns of Comorbidity in Hepatocellular Carcinoma: A Network Perspective

Hepatocellular carcinoma (HCC) is a common and fatal cancer. People with HCC report higher odds of comorbidity compared with people without HCC. To explore the association between HCC and medical comorbidity, we used routinely collected clinical data and applied a network perspective. In the network perspective, we used correlation analysis and community detection tests that described direct relationships among comorbidities. We collected 14,891 patients with HCC living in Jilin Province, China, between 2016 and 2018. Cirrhosis was the most common comorbidity of HCC. Hypertension and renal cysts were more common in male patients, while chronic viral hepatitis C, hypersplenism, hypoproteinemia, anemia and coronary heart disease were more common in female patients. The proportion of chronic diseases in comorbidities increased with age. The main comorbidity patterns of HCC were: HCC, cirrhosis, chronic viral hepatitis B, portal hypertension, ascites and other common complications of cirrhosis; HCC, hypertension, diabetes mellitus, coronary heart disease and cerebral infarction; and HCC, hypoproteinemia, electrolyte disorders, gastrointestinal hemorrhage and hemorrhagic anemia. Our findings provide comprehensive information on comorbidity patterns of HCC, which may be used for the prevention and management of liver cancer.
Quality by Design for the Development and Analysis of Enhanced In-Situ Forming Vesicles for the Improvement of the Bioavailability of Fexofenadine HCl In Vitro and In Vivo

Drug absorption from the gastrointestinal tract (GIT) is one of the major problems affecting the bioavailability of orally absorbed drugs. This work aims to enhance Fexofenadine HCl oral bioavailability in vivo, the drug used for allergic rhinitis. In this study, novel spray-dried lactose-based enhanced in situ forming vesicles were prepared using different absorption enhancer by the slurry method. Full factorial design was used to obtain an optimized formulation, while central composite design was used to develop economic, environment-friendly analysis method of Fexofenadine HCl in plasma of rabbits. The optimized formulation containing Capryol 90 as absorption enhancer has a mean particle size 202.6 ± 3.9 nm and zeta potential −31.6 ± 0.9 mV. It achieved high entrapment efficiency of the drug 73.7 ± 1.7% and rapid Q3h release reaches 71.5 ± 2.7%. The design-optimized HPLC assay method in rabbit plasma could separate Fexofenadine HCl from endogenous plasma compounds in less than 3.7 min. The pharmacokinetic study and the pharmacological effect of the fexofenadine-loaded optimized formulation showed a significant increase in blood concentration and significantly higher activity against compound 48/80 induced systemic anaphylaxis-like reactions in mice. Therefore, enhanced in situ forming vesicles were effective nanocarriers for the entrapment and delivery of Fexofenadine HCl.
Two Festuca Species—F. arundinacea and F. glaucescens—Differ in the Molecular Response to Drought, While Their Physiological Response Is Similar

Impact of photosynthetic and antioxidant capacities on drought tolerance of two closely related forage grasses, Festuca arundinacea and Festuca glaucescens, was deciphered. Within each species, two genotypes distinct in drought tolerance were subjected to a short-term drought, followed by a subsequent re-watering. The studies were focused on: (i) analysis of plant physiological performance, including: water uptake, abscisic acid (ABA) content, membrane integrity, gas exchange, and relative water content in leaf tissue; (ii) analysis of plant photosynthetic capacity (chlorophyll fluorescence; gene expression, protein accumulation, and activity of selected enzymes of the Calvin cycle); and (iii) analysis of plant antioxidant capacity (reactive oxygen species (ROS) generation; gene expression, protein accumulation and activity of selected enzymes). Though, F. arundinacea and F. glaucescens revealed different strategies in water uptake, and partially also in ABA signaling, their physiological reactions to drought and further re-watering, were similar. On the other hand, performance of the Calvin cycle and antioxidant system differed between the analyzed species under drought and re-watering periods. A stable efficiency of the Calvin cycle in F. arundinacea was crucial to maintain a balanced network of ROS/redox signaling, and consequently drought tolerance. The antioxidant capacity influenced mostly tolerance to stress in F. glaucescens.
Diurnal and seasonal variation of carbon dioxide exchange from a former true raised bog

Carbon dioxide exchange was measured, using the eddy covariance technique, during a one and a half year period in 1994 and 1995. The measurements took place over a former true raised bog, characterized by a shallow peat layer and a vegetation dominated by Molinia caerulea. The growing season extended from May until late October, with a maximum LAI in August of 1.7.
Deep Subdomain Adaptation Network for Image Classification

For a target task where the labeled data are unavailable, domain adaptation can transfer a learner from a different source domain. Previous deep domain adaptation methods mainly learn a global domain shift, i.e., align the global source and target distributions without considering the relationships between two subdomains within the same category of different domains, leading to unsatisfying transfer learning performance without capturing the fine-grained information. Recently, more and more researchers pay attention to subdomain adaptation that focuses on accurately aligning the distributions of the relevant subdomains. However, most of them are adversarial methods that contain several loss functions and converge slowly. Based on this, we present a deep subdomain adaptation network (DSAN) that learns a transfer network by aligning the relevant subdomain distributions of domain-specific layer activations across different domains based on a local maximum mean discrepancy (LMMD). Our DSAN is very simple but effective, which does not need adversarial training and converges fast. The adaptation can be achieved easily with most feedforward network models by extending them with LMMD loss, which can be trained efficiently via backpropagation. Experiments demonstrate that DSAN can achieve remarkable results on both object recognition tasks and digit classification tasks. Our code will be available at https://github.com/easezyc/deep-transfer-learning.
RESIDUAL IMPACT OF WELLGROW FORMULATION AND NPK ON GROWTH AND YIELD OF WHEAT (TRITICUM AESTIVUM L.)

Effect of concentrate organic manure (wellgrow grain and wellgrow soil) and NPK improved the growth and yield of wheat. Significant improvement in terms of growth parameters like plant height, tillers, dry matter production and productive tillers with application of 120 : 60 : 60 NPK + 300 kg wellgrow soil/ha and at par with application of 120 : 60 : 60 NPK + 300 kg wellgrow grain/ha, treatment receiving 100% NPK + 300 kg wellgrow soil/ha resulted maximum grain yield (4545 kg /ha). Treatment of 100% NPK + 300 kg wellgrow grain/ha maintained higher straw yield (5715 kg/ha) and test weight (44.10 g) due to application of 100 % NPK along with 200 kg wellgrow soil/ha whereas it was at par with application of 75 and 100% NPK with both levels of wellgrow formulation. DOI: http://dx.doi. org/10.3329/bjb.v44i1. 22738 Bangladesh J. Bot. 44 (1): 143-146, 2015 (March)
A New Saponin, Holothurin B, Isolated from Sea-Cucumber, Holothuria vagabunda and Holothuria lubrica

Two species of sea-cucumber were found to contain an unknown saponin component besides the major one identical with holothurin A isolated by Chanley from Actinopyga agassizi. The unknown component was newly isolated and designated holothurin B. It holds D-quinovose, D-xylose, sulfuric acid as the sodium salt, and presumably the same aglycon in common with holothurin A but lacks 3-O-methyl-D-glucose and D-glucose present in the latter.
Mice Carrying a Ubiquitous R235W Mutation of Wnt1 Display a Bone‐Specific Phenotype

Since a key function of Wnt1 in brain development was established early on through the generation of non‐viable Wnt1‐deficient mice, it was initially surprising that WNT1 mutations were found to cause either early‐onset osteoporosis (EOOP) or osteogenesis imperfecta type XV (OI‐XV). The deduced function of Wnt1 as an osteoanabolic factor has been confirmed in various mouse models with bone‐specific inactivation or overexpression, but mice carrying disease‐causing Wnt1 mutations have not yet been described. Triggered by the clinical analysis of EOOP patients carrying a heterozygous WNT1 mutation (p.R235W), we introduced this mutation into the murine Wnt1 gene to address the question of whether this would cause a skeletal phenotype. We observed that Wnt1+/R235W and Wnt1R235W/R235W mice were born at the expected Mendelian ratio and that they did not display postnatal lethality or obvious nonskeletal phenotypes. At 12 weeks of age, the homozygous presence of the Wnt1 mutation was associated with reduced trabecular and cortical bone mass, explained by a lower bone formation rate compared with wild‐type littermates. At 52 weeks of age, we also observed a moderate bone mass reduction in heterozygous Wnt1+/R235W mice, thereby underscoring their value as a model of WNT1‐dependent EOOP. Importantly, when we treated wild‐type and Wnt1+/R235W mice by daily injection of parathyroid hormone (PTH), we detected the same osteoanabolic influence in both groups, together with an increased cortical thickness in the mutant mice. Our data demonstrate the pathogenicity of the WNT1‐R235W mutation, confirm that controlling skeletal integrity is the primary physiological function of Wnt1, and suggest that osteoanabolic treatment with teriparatide should be applicable for individuals with WNT1‐dependent EOOP. © 2020 American Society for Bone and Mineral Research.