Miracle-dz/UniFAR-FTUs huggingface dataset:
A SlJRVEY OF FACTORS A.FFECTING TUPul\fOVER OF BOA .... W OF GO'VERNOR GRADUATE TEACHERS IN PUBLIC SECOl\TDARY SCHOOLS IN YJKUYU DISTRICT IN KENYA

Secondary school education development in Kenya has been one of the main concerns of the government since independence. Recommendations have been made by education reports, starting from the Ominde report (1964), on provision of sufficient and well trained teachers in the Kenyan schools. Besides supply of teachers, there have also been efforts to retain and motivate them in the teaching profession so as to provide quality education in secondary schools. Improvement of conditions of service for teachers is a promising way of increasing teacher morale, making the profession more attractive, enhancing retention of teachers, mitigating turn over, and improving the quality ofteaching and learning. The purpose ofthis study was to investigate the factors affecting turnover of board of governors' teachers in public secondary schools in Kikuyu district, Kenya. The objectives of the study were; to find out how compensation influence commitment and turnover of such teachers, to establish the effect oftheir working conditions, to determine the extent to which institution support to get placement in TSC affects their commitment and turnover, and finally to examine how further training affects commitment and turnover ofthe BOG teachers in Kikuyu district. The study adopted a descriptive survey design. Purposive random sampling technique was used to select principals while simple random sampling was used to pick BOG teachers from all the public secondary schools in Kikuyu district. A sample of78 respondents was selected from a target population of520 out ofwhich 60 responded to the questions. Closed ended questionnaires were used to collect data from respondents. Data analysis was facilitated by use of Statistical Package for Social Sciences (SPSS).
ModIs Dust AeroSol (MIDAS): A global fine resolution dust optical depth dataset

Abstract. Monitoring and describing the spatiotemporal variability of dust aerosols is crucial to understand their multiple effects, related feedbacks and impacts within the Earth system. This study describes the development of the MIDAS (ModIs Dust AeroSol) dataset. MIDAS provides columnar daily dust optical depth (DOD at 550 nm) at global scale and fine spatial resolution (0.1° × 0.1°) over a decade (2007–2016). This new dataset combines quality filtered satellite aerosol optical depth (AOD) retrievals from MODIS-Aqua at swath level (Collection 6, Level 2), along with DOD-to-AOD ratios provided by MERRA-2 reanalysis to derive DOD on the MODIS native grid. The uncertainties of MODIS AOD and MERRA-2 dust fraction with respect to AERONET and CALIOP, respectively, are taken into account for the estimation of the total DOD uncertainty (including measurement and sampling uncertainties). MERRA-2 dust fractions are in very good agreement with CALIOP column-integrated dust fractions across the dust belt, in the Tropical Atlantic Ocean and the Arabian Sea; the agreement degrades in North America and the Southern Hemisphere where dust sources are smaller. MIDAS, MERRA-2 and CALIOP DODs strongly agree when it comes to annual and seasonal spatial patterns; however, deviations of dust loads' intensity are evident and regionally dependent. Overall, MIDAS is well correlated with ground-truth AERONET-derived DODs (R = 0.882), only showing a small negative bias (−0.009 or −5.307 %). Among the major dust areas of the planet, the highest R values (up to 0.977) are found at sites of N. Africa, Middle East and Asia. MIDAS expands, complements and upgrades existing observational capabilities of dust aerosols and it is suitable for dust climatological studies, model evaluation and data assimilation.
Jukuri, open repository of the Natural Resources Institute Finland (Luke)

Environmental stress can affect individual development and fitness in insects. Forest logging is a serious environmental stress for forest-specialist insects, such as the mound-building wood ant Formica aquilonia Yarrow 1955, which builds its nests into forests and is dependent on the nutrition provided by the forest habitat. We studied whether the logging causes such a strong environmental stress that it would be visible in disproportionate growth and shape of young winged wood ant gynes (‘queens’) and males. We measured head width, thorax width and wing length of 144 gynes and 100 males from 12 nests from six clear-cut areas, 11 nests from unlogged forest stands and 10 nests in clear-cut—forest margins. We observed disproportionate growth of different body parts and allometric growth of wings in both sexes in areas with different logging-induced disturbance. Gynes had larger heads in clear-cut areas and males had narrowest thoraxes in forest clear-cut edges. With an increasing thorax width, the wing length decreases steeper in clear-cuts than in other habitats in gynes and increases steeper in clear-cuts than other habitats in males. It seems that in a strongly disturbed clear-cut environment, the gynes invest the growth of head width. There seems to be a trade-off between the growth of the thorax and wings in gynes but not in males. The altered body shape may be adaptation to clearings caused by storm events of wild fires, but not forest management practices of modern era. Large-headed gynes may be better in a colony take-over, needed for the establishment of new colonies in early succession stage habitats in this temporally social parasitic species. Long-winged males may have better long-range flight ability, and they may thus have better fitness and change to disperse their genes onto new habitats.
The Polarity of Online Reviews: Prevalence, Drivers and Implications

In this research, the authors investigate the prevalence, robustness, and possible reasons underlying the polarity of online review distributions, with the majority of the reviews at the positive end of the rating scale, a few reviews in the midrange, and some reviews at the negative end of the scale. Compiling a large data set of online reviews—over 280 million reviews from 25 major online platforms—the authors find that most reviews on most platforms exhibit a high degree of polarity, but the platforms vary in the degree of polarity on the basis of how selective customers are in reviewing products on the platform. Using cross-platform and multimethod analyses, including secondary data, experiments, and survey data, the authors empirically confirm polarity self-selection, described as the higher tendency of consumers with extreme evaluations to provide a review as an important driver of the polarity of review distributions. In addition, they describe and demonstrate that polarity self-selection and the polarity of the review distribution reduce the informativeness of online reviews.
Real-time structural motif searching in proteins using an inverted index strategy

Biochemical and biological functions of proteins are the product of both the overall fold of the polypeptide chain, and, typically, structural motifs made up of smaller numbers of amino acids constituting a catalytic center or a binding site. Detection of such structural motifs can provide valuable insights into the function(s) of previously uncharacterized proteins. Technically, this remains an extremely challenging problem because of the size of the Protein Data Bank (PDB) archive. Existing methods depend on a clustering by sequence similarity and can be computationally slow. We have developed a new approach that uses an inverted index strategy capable of analyzing >160,000 PDB structures with unmatched speed. The efficiency of the inverted index method depends critically on identifying the small number of structures containing the query motif and ignoring most of the structures that are irrelevant. Our approach (implemented at motif.rcsb.org) enables real-time retrieval and superposition of structural motifs, either extracted from a reference structure or uploaded by the user. Herein, we describe the method and present five case studies that exemplify its efficacy and speed for analyzing 3D structures of both proteins and nucleic acids. Author summary The Protein Data Bank (PDB) provides open access to more than 160,000 three-dimensional structures of proteins, nucleic acids, and biological complexes. Similarities between PDB structures give valuable functional and evolutionary insights but such resemblance may not be evident at sequence or global structure level. Throughout the database, there are recurring structural motifs – groups of modest numbers of residues in proximity that, for example, support catalytic activity. Identification of common structural motifs can unveil subtle similarities between proteins and serve as fingerprints for configurations such as the His-Asp-Ser catalytic triad found in serine proteases or the zinc coordination site found in Zinc Finger DNA-binding domains. We present a highly efficient yet flexible strategy that allows users for the first time to search for arbitrary structural motifs across the entire PDB archive in real-time. Our approach scales favorably with the increasing number and complexity of deposited structures, and, also, has the potential to be adapted for other applications in a macromolecular context.
Discovery of a Novel Inhibitor of Coronavirus 3CL Protease as a Clinical Candidate for the Potential Treatment of COVID-19

COVID-19 caused by the SARS-CoV-2 virus has become a global pandemic. 3CL protease is a virally encoded protein that is essential to the viral life cycle across a broad spectrum of coronaviruses with no close human analogs. The designed phosphate prodrug PF-07304814 is metabolized to PF-00835321 which is a potent inhibitor in vitro of the coronavirus family 3CL pro, with selectivity over human host protease targets. Furthermore, PF-00835231 exhibits potent in vitro antiviral activity against SARS-CoV-2 as a single agent and it is additive/synergistic in combination with remdesivir. We present the ADME, safety, and in vitro antiviral activity data to warrant clinical evaluation. One Sentence Summary The phosphate prodrug PF-07304814 is disclosed as an investigational novel intravenous small molecule 3CL protease inhibitor for COVID-19.
Why do Software Teams Deviate from Scrum? Reasons and Implications

Human, social, organizational, and technical aspects are intertwined with each other in software teams during the software development process. Practices that teams actually adopt often deviate from those of the used frameworks, such as Scrum. However, currently there is little empirical insight explaining typical deviations, including their reasons and consequences. In this paper we use observations to investigate selected activities of the software development process in two companies that use Scrum. We study identified deviations to understand their reasons and consequences, using a survey and interviews. We identify 13 deviations and we categorize reasons based on type. The deviations’ consequences are investigated in terms of their impact. Most deviations can be found in multiple teams. Reasons are doubts of the teams, organizational structures and complexity of the work. Consequences of deviations affect product development and team work.
The Effects of Mild Traumatic Brain Injury on Cognitive-Motor Integration for Skilled Performance

Adults exposed to blast and blunt impact often experience mild traumatic brain injury, affecting neural functions related to sensory, cognitive, and motor function. In this perspective article, we will review the effects of impact and blast exposure on functional performance that requires the integration of these sensory, cognitive, and motor control systems. We describe cognitive-motor integration and how it relates to successfully navigating skilled activities crucial for work, duty, sport, and even daily life. We review our research on the behavioral effects of traumatic impact and blast exposure on cognitive-motor integration in both younger and older adults, and the neural networks that are involved in these types of skills. Overall, we have observed impairments in rule-based skilled performance as a function of both physical impact and blast exposure. The extent of these impairments depended on the age at injury and the sex of the individual. It appears, however, that cognitive-motor integration deficits can be mitigated by the level of skill expertise of the affected individual, suggesting that such experience imparts resiliency in the brain networks that underly the control of complex visuomotor performance. Finally, we discuss the next steps needed to comprehensively understand the impact of trauma and blast exposure on functional movement control.
ATPase SRCAP is a new player in cell division, uncovering molecular aspects of Floating-Harbor syndrome

Floating-Harbor syndrome (FHS) is a rare genetic disease affecting human development caused by heterozygous truncating mutations in the Srcap gene, which encodes the ATPase SRCAP, the core catalytic subunit of the homonymous chromatin-remodeling complex. Using a combined approach, we studied the involvement of SRCAP protein in cell cycle progression in HeLa cells. In addition to the canonical localization in interphase nuclei, both SRCAP and its Drosophila orthologue DOMINO-A localized to the mitotic apparatus after nuclear envelope breakdown. Moreover, SRCAP and DOMINO-A depletion impaired mitosis and cytokinesis in human and Drosophila cells, respectively. Importantly, SRCAP interacted with several cytokinesis regulators at telophase, strongly supporting a direct role in cytokinesis, independent of its chromatin remodeling functions. Our results provide clues about previously undetected, evolutionarily conserved roles of SRCAP in ensuring proper mitosis and cytokinesis. We propose that perturbations in cell division contribute to the onset of developmental defects characteristic of FHS. Summary Significance statement Srcap is the causative gene of the rare Floating Harbor syndrome (FHS). It encodes the ATPase SRCAP, the core catalytic subunit of the homonymous multiprotein chromatin-remodeling complex in humans, which promotes the exchange of canonical histone H2A with the H2A.Z variant. According to the current view on SRCAP protein functions, FHS is caused by chromatin remodeling defects. Our findings suggest that, in addition to the established function as epigenetic regulator, SRCAP plays previously undetected and evolutionarily conserved roles in cell division. Hence, we propose that perturbations in cell division produced by SRCAP mutations are important causative factors co-occurring at the onset of FHS.
Refinement of a Cryo-EM Structure of hERG: Bridging Structure and Function

The human ether-a-go-go-related gene (hERG) encodes the voltage gated potassium channel (KCNH2 or Kv11.1, commonly known as hERG). This channel plays a pivotal role in the stability of phase 3 repolarization of the cardiac action potential. Although a high-resolution cryo-EM structure is available for its depolarized (open) state, the structure surprisingly did not feature many functionally important interactions established by previous biochemical and electrophysiology experiments. Using Molecular Dynamics Flexible Fitting (MDFF), we refined the structure and recovered the missing functionally relevant salt bridges in hERG in its depolarized state. We also performed electrophysiology experiments to confirm the functional relevance of a novel salt bridge predicted by our refinement protocol. Our work shows how refinement of a high-resolution cryo-EM structure helps to bridge the existing gap between the structure and function in the voltage-sensing domain (VSD) of hERG. Statement of Significance Cryo-EM has emerged as a major breakthrough technique in structural biology of membrane proteins. However, even high-resolution Cryo-EM structures contain poor side chain conformations and interatomic clashes. A high-resolution cryo-EM structure of hERG1 has been solved in the depolarized (open) state. The state captured by Cryo-EM surprisingly did not feature many functionally important interactions established by previous experiments. Molecular Dynamics Flexible Fitting (MDFF) used to enable refinement of the hERG1 channel structure in complex membrane environment re-establishing key functional interactions in the voltage sensing domain.
Adherence to Human Colon Cells by Multidrug Resistant Enterobacterales Strains Isolated From Solid Organ Transplant Recipients With a Focus on Citrobacter freundii

Enterobacteria species are common causes of hospital-acquired infections, which are associated with high morbidity and mortality rates. Immunocompromised patients such as solid organ transplant (SOT) recipients are especially at risk because they are frequently exposed to antibiotics in the course of their treatments. In this work, we used a collection of 106 Escherichia coli, 78 Klebsiella pneumoniae, 25 Enterobacter spp. , and 24 Citrobacter spp. multidrug resistant strains isolated from transplant patients (hepatic, renal or renal/pancreatic) in order to examine their ability to adhere in vitro to HT-29 human colon cells, and to determine if some adhesive characteristics are associated with prevalence and persistence of these strains. A total of 33 E. coli (31%), 21 K. pneumoniae (27%), 7 Enterobacter spp. (28%), and 5 Citrobacter spp. (21%), adhered to the colon epithelial cells. Two main adherence patterns were observed in the four species analyzed, diffuse adherence, and aggregative adherence. Under transmission electronic microscopy (TEM), most bacteria lacked visible fimbria on their surface, despite their strong adherence to epithelial cells. None of the strains studied was able to induce any cytotoxic effect on HT-29 cells although some of them strongly colonizing both cells and glass coverslips at high density. Some of the strains failed to adhere to the epithelial cells but adhered strongly to the cover-slide, which shows that microscopy studies are mandatory to elucidate the adherence of bacteria to epithelial cells in vitro, and that quantitative assays using colony forming unit (CFUs) counting need to be supplemented with pictures to determine definitively if a bacterial strain adheres or not to animal cells in vitro. We report here, for the first time, the aggregative adherence pattern of two multidrug resistant (MDR) Citrobacter freundii strains isolated from human patients; importantly, biofilm formation in Citrobacter is totally dependent on the temperature; strong biofilms were formed at room temperature (RT) but not at 37°C, which can play an important role in the colonization of hospital surfaces. In conclusion, our results show that there is a great variety of adhesion phenotypes in multidrug-resistant strains that colonize transplanted patients.
Microstructural Integrity of the Hippocampus During Childhood: Relations With Age and Source Memory

The hippocampus is a brain structure known to be important for memory. However, studies examining relations between hippocampal volume and memory across development yield mixed results. This may be due in part to the fact that volume is a coarser measure of hippocampal composition. Studies have begun to examine measures of diffusion, which capture characteristics of the microstructure of the hippocampus, and thus may provide additional information about the integrity of the underlying neural circuits. The present study applied this approach to a developmental period characterized by dramatic changes in both hippocampal microstructure and memory behavior – early childhood. Specifically, measures of hippocampal microstructural integrity were related to age and source memory performance in 93 children aged 4–8 years. Results revealed significant negative associations between hippocampal mean diffusivity and both age and memory, even after controlling for differences in hippocampal volume. These results suggest that hippocampal diffusion may provide additional, independent information about hippocampal integrity compared to volume, particularly during early childhood when important developmental changes have been proposed.
Superficial Siderosis and Microbleed Restricted in Cortex Might Be Correlated to Atrophy and Cognitive Decline in Sneddon's Syndrome

Objective: Sneddon's syndrome is a rare non-inflammatory arteriopathy affecting small and medium-sized arteries, characterized by a generalized livedo reticularis and recurrent transient ischemic attack or ischemic stroke. Hemorrhagic stroke was reported in limited cases, but microbleeds and superficial siderosis were rarely issued. We aimed to investigate the hemorrhagic imaging features of Sneddon's syndrome and explore the possible mechanism and clinical relevance. Methods: Clinical data and cerebral MR imaging including T2* sequence of seven consecutive patients with Sneddon's syndrome were reviewed. Results: The most common neurological manifestations were cognitive impairment and stroke attack (71.4%), followed by seizures and movement disorder (28.6%). Cerebral microbleeds were detected in six patients on T2* sequence, all of them presented with cortical microbleeds, only one of them with microbleeds in basal ganglion. More than five microbleeds were observed in four of these six patients. The majority of the microbleeds were predominantly cortical restricted and especially located in the cortical watersheds. Multiple superficial siderosis were identified mainly involving cortical watersheds in five cases. Significant cerebral atrophy with prominent secondary white matter hyperintensities in bilateral cortical watersheds were also observed. Abnormal tortuous and multiple focal occlusion of bilateral distal MCA were shown in one patient by DSA. No stenosis of proximal segment of cerebral arteries was detected in all the patients. Conclusions: This is the first report illustrating abundant cortical microbleeds and superficial siderosis mainly involved the anterior and posterior cortical watersheds in Sneddon's syndrome. The surprisingly identical topographic distribution of hemorrhagic lesions and the obvious atrophy suggest cerebral atrophy might be secondary to the microangiopathy related hemorrhagic lesions and further contribute to the neurological deficit, especially the early cognitive decline in Sneddon syndrome.
When Salt Meddles Between Plant, Soil, and Microorganisms

In extreme environments, the relationships between species are often exclusive and based on complex mechanisms. This review aims to give an overview of the microbial ecology of saline soils, but in particular of what is known about the interaction between plants and their soil microbiome, and the mechanisms linked to higher resistance of some plants to harsh saline soil conditions. Agricultural soils affected by salinity is a matter of concern in many countries. Soil salinization is caused by readily soluble salts containing anions like chloride, sulphate and nitrate, as well as sodium and potassium cations. Salinity harms plants because it affects their photosynthesis, respiration, distribution of assimilates and causes wilting, drying, and death of entire organs. Despite these life-unfavorable conditions, saline soils are unique ecological niches inhabited by extremophilic microorganisms that have specific adaptation strategies. Important traits related to the resistance to salinity are also associated with the rhizosphere-microbiota and the endophytic compartments of plants. For some years now, there have been studies dedicated to the isolation and characterization of species of plants’ endophytes living in extreme environments. The metabolic and biotechnological potential of some of these microorganisms is promising. However, the selection of microorganisms capable of living in association with host plants and promoting their survival under stressful conditions is only just beginning. Understanding the mechanisms of these processes and the specificity of such interactions will allow us to focus our efforts on species that can potentially be used as beneficial bioinoculants for crops.