Miracle-dz/UniFAR-FTUs huggingface dataset:
Chemical genetic interaction linking eIF5A hypusination and mitochondrial integrity

The eukaryotic translation factor eIF5A plays an important role in translation elongation, especially across stretches of prolines and charged amino acids, and in translation termination. eIF5A undergoes hypusination, a post-translational modification unique to this protein, although the role of hypusination in the function of eIF5A remains elusive. Here, we investigated the cellular defects induced by the hypusination inhibitor GC7 (N1-guanyl-1,7-diaminoheptane). Proteome, translatome, and transcriptome analyses indicated that GC7 downregulated a subset of mitochondrial proteins and DNA, causing mitochondrial stress and eliciting the integrated stress response. Chemical genomic screening using barcoded shRNA libraries identified genes encoding proteins involved in polyamine metabolism/transport and MPV17L2, a mitochondrial disease gene homologue whose product regulates mitochondrial translation. Depletion of MPV17L2 caused hypersensitivity to GC7 and phenocopied the effects of GC7 treatment. These results suggest that eIF5A hypusination and MPV17L2 synthetically regulate mitochondrial molecular synthesis and integrity.
Habitat selection of farmland feeding geese in West Jutland, Denmark: an example of a niche shift

Etude du choix de l'habitat chez Anser brachyrhynchus et Anser anser qu'ils soient en sympatrie temporaire ou en allopatrie, dans des zones agricoles de l'ouest du Jutland. En dehors du type d'habitat, le degre de perturbation et la tolerance a cette derniere sont des facteurs importants de segregation des deux especes
THE IMPACT OF EMPLOYEE COMMUNICATION AND PERCEIVED EXTERNAL PRESTIGE ON ORGANIZATIONAL IDENTIFICATION

Employees' organizational identification was measured in three organizations. Results show that employee communication augments perceived external prestige and helps explain organizational identification. Communication climate plays a central role, mediating the impact on organizational identification of the content of communication. The relative impacts of employee communication and perceived external prestige on organizational identification differ between organizations; this was attributed to differences in reputation of the companies. Consequences of the results for the management of organizational identification are discussed
A structured approach for building multi-community state-and-transition models 1 to support conservation planning 2

31 The global decline in the extent and condition of ecological communities has resulted in an increasing 32 demand for recovery and conservation plans. Conservation plans for ecological communities require a 33 management framework with measurable, time-bound objectives, a targeted management strategy, and 34 indicators that enable actions to be evaluated in relation to objectives. Methods that allow for the transfer 35 of knowledge among similar systems and facilitate consistent and comparable plans are essential, especially 36 when resources are constrained. We describe a process to streamline the development of conservation 37 plans by combining functionally similar community sub-types into a multi-community State and Transition 38 Model. We demonstrate this approach in a case study where we use the combined expertise of Australian 39 ecologists to build a multi-community State and Transition Model for eucalypt woodlands of southern 40 Australia – an ecosystem which occupies a vast geographical range across temperate Australia and includes 41 many distinct vegetation communities, a growing number of which are endangered or threatened. We 42 identify commonalities and differences among three broad woodland sub-types including a set of eight 43 general condition states, a list of drivers of transitions among condition states, and the uncertainties and 44 time-frames associated with each transition. Two key findings across all models are that management is 45 state-dependent, and transition directly to the ‘ Exemplar ’ state from any other state is considered highly 46 unlikely. Other examples of State and Transition Models in the literature are focused on single communities 47 or a significantly smaller scale, and this is the first attempt to construct a nationally relevant multi-48 community State and Transition Model via a structured and consultative process. Based on this case study, 49 we propose a repeatable protocol for developing multi-community State and Transition Models. This 50 process could improve and streamline the development of robust conservation plans for threatened 51 ecological communities more broadly.
HR Development Strategy Through Training Towards Superior Tourism on the Department of Tourism, Youth and Sports at

Human resources are an asset or driving force in an organization. This study aims to analyze the strategy for developing human resources in tourism sector on the Department of Tourism, Youth and Sports at Bulukumba Regency. This study used qualitative method with a case study type. The data collection techniques used observation, interviews and documentation with 14 informants (8 employees from Department of Tourism, Youth and Sports at Bulukumba Regency, 2 employees from Personnel and Human Resources Development Agency Office and 4 people from community). Data analysis techniques were assisted by software NVivo 12 plus. The results of this research indicate that HR development strategies in the tourism sector can be carried out by improving the training process. The recruitment process requires an even distribution of employees so that the strength of human resources becomes more balanced. Through formal education, employees have a basic starting point so they can easily complete the work they are entrusted with. However, when an employee has an educational background that is not appropriate to the fieldwork can be compensated by some experiences that have been undertaken and following training that can develop the employee's skills. Besides, the role of the community was very important for the progress of tourism in Bulukumba Regency, the community has a lot of participation in developing tourist attractions in Bulukumba, both in the form of ecotourism managed by the local village, cultural tourism, and also existing marine tourism.
Socioeconomic status, non-communicable disease risk factors, and walking speed in older adults: multi-cohort population based study

OBJECTIVE To assess the association of low socioeconomic status and risk factors for non-communicable diseases (diabetes, high alcohol intake, high blood pressure, obesity, physical inactivity, smoking) with loss of physical functioning at older ages. DESIGN Multi-cohort population based study. SETTING 37 cohort studies from 24 countries in Europe, the United States, Latin America, Africa
Genotype by Environment Interaction and Yield Stability of Drought Tolerant Mung Bean [ Vigna radiata (L.) Wilczek] Genotypes in Ethiopia

: A multi-environment evaluation of mung bean genotypes was conducted in six environments across Ethiopia to select promising genotypes. This study was conducted to estimate the magnitude of genotypes by environment interaction (GEI) and seed yield stability of the selected drought-tolerant mung bean genotypes across different environments. A total of fifteen mung bean genotypes were used. Out of these, two released varieties were used as standard checks. The field experiments were conducted during the 2019 main cropping season at six locations namely Humbo, Gofa, Melkassa, Konso, Jinka, and Kako using a randomized complete block design with three replications. Data were subjected to analysis of variance, Additive Main Effects and Multiplicative Interaction (AMMI), and GGE bi-plot analysis. A combined analysis of variance revealed significant variations among the genotype, environments, and GEI for yield and yield-related traits, indicating that seed yield was significantly affected by these factors. Analysis of variance from the AMMI model indicated the contribution of environment, genotype, and GEI was 59.6%, 16.8%, and 14.8% of the total variation in seed yield, respectively. Sum squares of the first and the second interaction principal component axis (IPCA) explained 47.4% and 7.4% of the GEI variation, respectively. The IPCA1 mean square was highly significant (P≤0.01) and that of IPCA2 was significant (p≤0.05), indicating the adequacy of the AMMI model with the first two IPCAs for cross-validation of the seed yield variation. The magnitude of the GEI sum squares was 4.4 times that of the genotypes sum squares for seed yield, indicating the presence of substantial differences in genotypic responses across the environments. The results for the AMMI, Yield stability index (YSI), AMMI Stability Value (ASV), and GGE biplot, analyses depicted that the genotypes G6 (NLLP-MGC-24), G13 (Acc006), and G3 (NLLP-MGC-15) were identified as stable and high yielders across the environments and should be considered for variety release. AMMI1 biplot showed Kako was the potential and favorable environment for mung bean production, while Humbo was an unfavorable for mung bean production.
Estimation of Canada’s methane emissions: inverse modelling analysis using the ECCC measurement network

Canada has major sources of atmospheric methane (CH 4 ), with the world second-largest boreal wetland and the world fourth-largest natural gas production. However, Canada’s CH 4 emissions remain uncertain among estimates. Better quantification and characterization of Canada’s CH 4 emissions are critical for climate mitigation strategies. To improve our understanding of Canada’s CH 4 emissions, we performed an ensemble regional inversion (2007–2017) constrained with the 10 Environment and Climate Change Canada (ECCC) surface measurement network. The decadal CH 4 estimates show no significant trend, unlike some studies that reported long-term trends. The total CH 4 estimate is 17.4 (15.3–19.5) Tg CH 4 year - 1 , partitioned into natural and anthropogenic sources, 10.8 (7.5–13.2) and 6.6 (6.2–7.8) Tg CH 4 year -1 , respectively. The estimated anthropogenic emission is higher than inventories, mainly in western Canada (with the fossil fuel industry). Furthermore, the results reveal notable spatiotemporal characteristics. First, the modelled gradients of atmospheric CH 4 show 15 improvement after inversion when compared to observations, implying the CH 4 gradients could help verify the inversion results. Second, the seasonal variations show slow onset and late summer maximum, indicating wetland CH 4 flux has hysteretic dependence on air temperature. Third, the boreal winter natural CH 4 emissions, usually treated as negligible, appear quantifiable (≥ 20 % of annual emissions). Understanding winter emission is important for climate prediction, as the winter i n Canada is warming faster than the summer. Fourth, the inter-annual variability in estimated CH 4 emissions is positively 20 correlated with summer air temperature anomalies. This could enhance Canada’s
MERCURY DISTRIBUTIONS AND CYCLING IN THE NORTH ATLANTIC AND EASTERN TROPICAL PACIFIC OCEANS

Mercury (Hg) in the ocean undergoes transformations in the water column including reduction to elemental Hg (Hg 0 ) and methylation to dimethylmercury ((CH3)2Hg, DMHg) and monomethylmercury (CH3Hg + , MMHg). Monomethylmercury is a neurotoxin that bioaccumulates in predatory fish species harvested for human consumption. I participated in the U.S. GETORACES section of the eastern tropical Pacific Ocean (GP16) to examine filtered and particulate total Hg (HgT) and MMHg, and dissolved gaseous Hg 0 and DMHg with high-resolution vertical profiles at 35 sampling stations. Total Hg had a nutrient-like vertical distribution with increasing concentrations in aging Pacific deep waters. Concentrations of filtered HgT, MMHg and DMHg also increased with age among deep water masses unaffected by anthropogenic Hg. Unlike in the Atlantic Ocean, a large hydrothermal plume stemming from the East Pacific Rise was not enriched with Hg relative to surrounding deep waters. Waters within 1000 m of abyssal sediments contained more HgT than expected from remineralization of sinking particles. Geothermal heating along the rise may increase the flux of Hg from sediments, accounting for the excess Hg. The behavior of HgT surrounding the East Pacific Rise may be unique to fast spreading centers in the ocean. In thermocline waters, elemental Hg was inversely correlated with N*, a proxy for denitrification, suggesting that denitrifying bacteria (pseudomonads, e.g.) may also be capable of Hg reduction. The cruise section
Transcranial focused ultrasound to human rIFG improves response inhibition through modulation of the P300 onset latency

Response inhibition in humans is important to avoid undesirable behavioral action consequences. Neuroimaging and lesion studies point to a locus of inhibitory control in the right inferior frontal gyrus (rIFG). Electrophysiology studies have implicated a downstream event-related potential from rIFG, the fronto-central P300, as a putative neural marker of the success and timing of inhibition over behavioral responses. However, it remains to be established whether rIFG effectively drives inhibition and which aspect of P300 activity uniquely indexes inhibitory control—ERP timing or amplitude. Here, we dissect the connection between rIFG and P300 for inhibition by using transcranial-focused ultrasound (tFUS) to target rIFG of human subjects while they performed a Stop-Signal task. By applying tFUS simultaneously with different task events, we found behavioral inhibition was improved, but only when applied to rIFG simultaneously with a ‘stop’ signal. Improved inhibition through tFUS to rIFG was indexed by faster stopping times that aligned with significantly shorter N200/P300 onset latencies. In contrast, P300 amplitude was modulated during tFUS across all groups without a paired change in behavior. Using tFUS, we provide evidence for a causal connection between anatomy, behavior, and electrophysiology underlying response inhibition.
Individual differences in ﬁeld independence inﬂuence the ability to determine accurate needle angles

Objective: ‘‘Field dependence’’ is used in cognitive psychology to describe an individual’s tendency to be visually distracted by the surrounding environment. Notwithstanding the role of ﬁeld dependence in contexts in which spatial judgment is important, such as piloting an aircraft, to date, studies linking ﬁeld dependence to surgical skills have been limited. We evaluated whether ﬁeld dependence correlates with an ability to anticipate appropriate needle angles in a simulated setting. Methods: Trainees underwent ﬁeld dependence testing and then participated in a surgical skills exercise. Correlations betweenﬁeld dependence and surgicalskill were computed. Speciﬁcally, cardiothoracicsurgeryres-idents (n ¼ 11) took a battery of cognitive examinations to assess general reasoning and visuospatial judgment. Two written tests, the Rod and Frame test and the Judgment of Line Orientation test, measured the degree of ﬁeld dependence. The subjects then underwent surgical skills testing. Using a standard needle driver, the participants placed curved needles into a cylindrical silicone mitral valve model with 10 premarked needle entry and exit sites. The components assessed included the ability to load a needle on driver at the appropriate angle. Results: The test results showed a parametric distribution, with internal cognitive testing controls demonstrating valid testing techniques and methods. Performance on the cognitive tests measuring spatial judgment and ﬁeld dependence correlated signiﬁcantly with skill at determining the appropriate needle angle load in an inverse fashion (Judgment of Line Orientation test, r ¼ 0.61, P < .05; Rod and Frame test, r ¼ (cid:2) 0.52, P ¼ . 05), suggesting that residents who were not distracted by surrounding objects performed better. Performance on the cognitive examinations did not correlate with resident training level. Conclusions: Although our study was of a small cohort, the ﬁndings suggest that individuals described as ﬁeld independent (not easily distracted by external visual cues) might possess improved ability to determine appropriate needle angle loads compared with ﬁeld-dependent individuals. Additional studies examining the
Algorithmic Operations Research From Balls and Bins to Points and Vertices

Given a graph G = ( V, E ) with | V | = n , we consider the following problem. Place m = n points on the vertices of G independently and uniformly at random. Once the points are placed, relocate them using a bijection from the points to the vertices that minimizes the maximum distance between the random place of the points and their target vertices.We look for an upper bound on this maximum relocation distance that holds with high probability (over the initial placements of the points). For general graphs and in the case m ≤ n , we prove the # P -hardness of the problem and that the maximum relocation distance is O ( √ n ) with high probability. We present a Fully Polynomial Randomized Approximation Scheme when the input graph admits a polynomial-size family of witness cuts while for trees we provide a 2 -approximation algorithm. Many applications concern the variation in which m = (1 − ǫ ) n for some 0 < ǫ < 1 . We provide several bounds for the maximum relocation distance according to different graph topologies.
Effect of anesthesia level on murine cardiac function [version 1; peer review: 1 approved, 1 approved with reservations]

Background: Echocardiography allows for sensitive and non-invasive assessment of cardiac function in mice, but requires sedation and immobility, which influences cardiac performance. Minimizing the hemodynamic effects of anesthesia is extremely important for improving the applicability of animal models to the clinical setting. We sought to evaluate the effects of isoflurane dose on myocardial function in a murine model. Methods: Twelve healthy C57BL/6 mice were studied with three different isoflurane anesthesia regimens: deep anesthesia with an objective of heart rate (HR) between 350 and 400 beats per minute (bpm), light anesthesia with an objective of HR between 475 and 525 bpm and just before the mice woke up (>575 bpm). Using a high-resolution ultrasound system, stroke volume, cardiac output, left ventricle dimension and fractional shortening were recorded. Results: Fractional shortening was not statistically different in the awake group and the light anesthesia group (49±5% in awake mice vs. 48±5%; p=0.62), whereas it was different compared to the deep anesthesia group (31±5%, p<0.0001 compared to both groups). Similar results were found for stroke volume (41.4±5.8 ml vs. 41.6±6.9 ml; p=0.81 and 35±8
The TOP-100 most dangerous invasive alien species in Northern Eurasia: invasion trends and species distribution modelling

Northern Eurasia is extensive and includes terrestrial and aquatic ecosystems that cover several natural zones and access to the seas of three oceans. As a result, it has been invaded by numerous invasive alien species (IAS) over large temporal and spatial scales. The purpose of this research was to assess invasion trends and construct species distribution models for the Russian TOP-100 most dangerous IAS. Environmentally suitable regions for IAS were established based on alien species attribute databases, datasets of 169,709 species occurrence records (SOR) and raster layers of environmental variables using species distribution modelling (MaxEnt). The objectives of this research were to (1) create databases of SOR for the TOP-100 IAS in Russia; 2) determine pathways, residence time, donor regions and trends of invasions; (3) determine the main types of spatial distributions of invasive species and their relation to residence time; and (4) distinguish regions with the highest richness of IAS that have a strong impact on the terrestrial and aquatic ecosystems of Russia. We found that although species invasions date back over 400 years, the number of naturalized IAS has increased non-linearly over
high-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis

: Purulent meningitis (PM) is a severe infectious disease that is associated with high rates of morbidity and mortality. It has been recognized that bacterial infection is a major contributing factor to the pathogenesis of PM. However, there is a lack of information on the bacterial composition in PM, due to the low positive rate of cerebrospinal fluid bacterial culture. Herein, we aimed to discriminate and identify the main pathogens and bacterial composition in cerebrospinal fluid sample from PM patients using high-throughput sequencing approach. The cerebrospinal fluid samples were collected from 26 PM patients, and were determined as culture-negative samples. The polymerase chain reaction products of the hypervariable regions of 16S rDNA gene in these 26 samples of PM were sequenced using the 454 GS FLX system. The results showed that there were 71,440 pyrosequencing reads, of which, the predominant phyla were Proteobacteria and Firmicutes; and the predominant genera were Streptococcus, Acinetobacter, Pseudomonas , and Neisseria . The bacterial species in the cerebrospinal fluid were complex, with 61.5% of the samples presenting with mixed pathogens. A significant number of bacteria belonging to a known pathogenic potential was observed. The number of operational taxonomic units for individual samples ranged from six to 75 and there was a comparable difference in the species diversity that was calculated through alpha and beta diversity analysis. Collectively, the data show that high-throughput sequencing approach facilitates the characterization of the pathogens in cerebrospinal fluid and determine the abundance and the composition of bacteria in the cerebrospinal fluid samples of the PM patients, which may provide a better understanding of pathogens in PM and assist clinicians to make rational and effective therapeutic decisions. of bacterial communities in the cerebrospinal fluid in patients with culture-negative PM using 16S rDNA Amplicon 454