Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of nanoparticles on biological contamination of in vitro cultures and organogenic regeneration of banana.

This study aimed to identify biological contaminants of banana in vitro cultures. We also tested the effect of Zn or ZnO nanoparticles on elimination of some bacterial and fungal contaminants and their influence regeneration. Nine strains of bacterial contaminants (Cellulomonas uda, Cellulomonas flarigena, Corynebacterium panrometabolum, Bacillus megaterium, Staphylococcus spp., Klebsiella spp., Erwinia cypripedii, Pseudomonas spp. and Proteus spp.) and four fungal (Fusarium spp., Aspergillus spp., Penicillium spp. and Candida spp.) contaminants were identified in nanoparticles-free media of banana in vitro cultures. They eventually led the explants death. The contamination-free cultures of banana in vitro cultures were obtained as a result of application of nano Zn and ZnO particles to the culture MS media, with no negative effect on regeneration. The callus growth decreased while total proline as well as SOD, CAT and POX activities were increased significantly, when the nanoparticles doses increased. The highest percent of somatic embryogenesis was observed in MS media supplemented with 100 mg/L nano Zn followed by nano ZnO. Excellent shooting, rooting and regenerated plantlets were observed also in MS+100 mg/L nano Zn and ZnO. Regenerated plantlets were successfully acclimatized with about 98% efficiency for the experimental period (one month). Nanoparticles treated somaclones accumulated more proline, chlorophyll, antioxidant enzymes activity and developed more dry weight accumulation than the control. In conclusion, the microbial contaminants in banana in vitro culture can effectively be eliminated by incorporation of nano Zn and nano ZnO particles on growth media at different concentrations. However, 100 mg/L dose was preferable because it showed the best effects on increasing the regeneration of plantlets with well-formed root systems. Further studies are needed to investigate the mechanisms and the side effects of nanoparticles on genetic stability of banana in vitro cultures.
Applying prosodic speech features in mental health care: An exploratory study in a life-review intervention for depression

The present study aims to investigate the application of prosodic speech features in a psychological intervention based on lifereview. Several studies have shown that speech features can be used as indicators of depression severity, but these studies are mainly based on controlled speech recording tasks instead of natural conversations. The present exploratory study investigated speech features as indicators of depression in conversations of a therapeutic intervention. The changes in the prosodic speech features pitch, duration of pauses, and total duration of the participant’s speaking time were studied over four sessions of a life-review intervention for three older participants. The ecological validity of the dynamics observed for prosodic speech features could not be established in the present study. The changes in speech features differed from what can be expected in an intervention that is effective in decreasing depression and were inconsistent with each other for each of the participants. We suggest future research to investigate changes within the intervention sessions, to relate the changes in feature values to the topical content of the speech, and to relate the speech features directly to depression scores.
Arabidopsis ERG28 Tethers the Sterol C4-Demethylation Complex to Prevent Accumulation of a Biosynthetic Intermediate That Interferes with Polar Auxin Transport[C][W]

Sterol biosynthetic intermediates (SBIs) derived from the sterol C4-demethylation complex tethered by ERG28 represent signaling molecules in mammals and fungi. In Arabidopsis, loss of ERG28 function leads to the accumulation of the previously undetected SBI, 4-carboxy-4-methyl-24-methylenecycloartanol, which inhibits polar auxin transport. Our work provides a link between sterol metabolism and auxin transport. Sterols are vital for cellular functions and eukaryotic development because of their essential role as membrane constituents. Sterol biosynthetic intermediates (SBIs) represent a potential reservoir of signaling molecules in mammals and fungi, but little is known about their functions in plants. SBIs are derived from the sterol C4-demethylation enzyme complex that is tethered to the membrane by Ergosterol biosynthetic protein28 (ERG28). Here, using nonlethal loss-of-function strategies focused on Arabidopsis thaliana ERG28, we found that the previously undetected SBI 4-carboxy-4-methyl-24-methylenecycloartanol (CMMC) inhibits polar auxin transport (PAT), a key mechanism by which the phytohormone auxin regulates several aspects of plant growth, including development and responses to environmental factors. The induced accumulation of CMMC in Arabidopsis erg28 plants was associated with diagnostic hallmarks of altered PAT, including the differentiation of pin-like inflorescence, loss of apical dominance, leaf fusion, and reduced root growth. PAT inhibition by CMMC occurs in a brassinosteroid-independent manner. The data presented show that ERG28 is required for PAT in plants. Furthermore, it is accumulation of an atypical SBI that may act to negatively regulate PAT in plants. Hence, the sterol pathway offers further prospects for mining new target molecules that could regulate plant development.
The Effects of 4-Methylethcathinone on Conditioned Place Preference, Locomotor Sensitization, and Anxiety-Like Behavior: A Comparison with Methamphetamine

Background: 4-Methylethcathinone is a drug that belongs to the second generation of synthetic cathinones, and recently it has been ranked among the most popular “legal highs”. Although it has similar in vitro neurochemical actions to other drugs such as cocaine, the behavioral effects of 4-methylethcathinone remain to be determined. Methods: The addictive potential and locomotor potentiation by 4-methylethcathinone were investigated in rats using the conditioned place preference and sensitization paradigm. Methamphetamine was used as a positive control. Because synthetic cathinones can have psychological effects, we also examined anxiety-like behavior using the elevated plus maze. Results: A conditioning dose of 10mg/kg 4-methylethcathinone was able to induce conditioned place preference and reinstatement (following 2 weeks of withdrawal). Acute or repeated injections of 4-methylethcathinone at 3 or 10mg/kg failed to alter locomotor activity. At 30mg/kg, however, acute 4-methylethcathinone increased locomotor activity compared with saline, while chronic 4-methylethcathinone induced a delayed and attenuated sensitization compared with methamphetamine. Additionally, repeated daily injections of 4-methylethcathinone (30mg/kg) reduced, whereas methamphetamine increased time spent by rats in the open arm of an elevated plus maze compared with saline injections. Interestingly, a 2-week withdrawal period following chronic injections of 4-methylethcathinone or methamphetamine increased time spent in the open arm in all rats. Conclusions: The rewarding properties of 4-methylethcathinone were found to be dissociated from its effects on locomotor activity. Additionally, chronic 4-methylethcathinone use may trigger abnormal anxious behaviors. These behavioral effects caused by 4-methylethcathinone appear to last even after a withdrawal period.
USER INTERFACES

The user interface is that part of a computer system through which human user and computer communicate. With the increasing prevalence of interactive personal computer systems the importance of human communication is growing steadily, and many systems now stand or fall on the quality of their interfaces. Interfaces consume a large amount of software construction and maintenance effort—estimates of the fraction of an interactive system’s code devoted to the user interface vary from one third to almost two thirds.
Towards robotic visual and acoustic stealth for outdoor dynamic target tracking

Covertly tracking mobile targets, either animal or human, in previously unmapped outdoor natural environments using off-road robotic platforms requires both visual and acoustic stealth. Whilst the use of robots for stealthy surveillance is not new, the majority only consider navigation for visual covertness. However, most fielded robotic systems have a non-negligible acoustic footprint arising from the onboard sensors, motors, computers and cooling systems, and also from the wheels interacting with the terrain during motion. This time-varying acoustic signature can jeopardise any visual covertness and needs to be addressed in any stealthy navigation strategy. In previous work, we addressed the initial concepts for acoustically masking a tracking robot’s movements as it travels between observation locations selected to minimise its detectability by a dynamic natural target and ensuring con- tinuous visual tracking of the target. This work extends the overall concept by examining the utility of real-time acoustic signature self-assessment and exploiting shadows as hiding locations for use in a combined visual and acoustic stealth framework.
A Web-Based and Mobile Health Social Support Intervention to Promote Adherence to Inhaled Asthma Medications: Randomized Controlled Trial

Background Online communities hold great potential as interventions for health, particularly for the management of chronic illness. The social support that online communities can provide has been associated with positive treatment outcomes, including medication adherence. There are few studies that have attempted to assess whether membership of an online community improves health outcomes using rigorous designs. Objective Our objective was to conduct a rigorous proof-of-concept randomized controlled trial of an online community intervention for improving adherence to asthma medicine. Methods This 9-week intervention included a sample of asthmatic adults from the United Kingdom who were prescribed an inhaled corticosteroid preventer. Participants were recruited via email and randomized to either an “online community” or “no online community” (diary) condition. After each instance of preventer use, participants (N=216) were required to report the number of doses of medication taken in a short post. Those randomized to the online community condition (n=99) could read the posts of other community members, reply, and create their own posts. Participants randomized to the no online community condition (n=117) also posted their medication use, but could not read others’ posts. The main outcome measures were self-reported medication adherence at baseline and follow-up (9 weeks postbaseline) and an objective measure of adherence to the intervention (visits to site). Results In all, 103 participants completed the study (intervention: 37.8%, 39/99; control: 62.2%, 64/117). MANCOVA of self-reported adherence to asthma preventer medicine at follow-up was not significantly different between conditions in either intention-to-treat (P=.92) or per-protocol (P=.68) analysis. Site use was generally higher in the control compared to intervention conditions. Conclusions Joining an online community did not improve adherence to preventer medication for asthma patients. Without the encouragement of greater community support or more components to sustain engagement over time, the current findings do not support the use of an online community to improve adherence. ClinicalTrial International Standard Randomized Controlled Trial Number (ISRCTN): 29399269; http://www.isrctn. com/ISRCTN29399269/29399269 (Archived by WebCite at http://www.webcitation.org/6fUbEuVoT)
Recognition of geochemical footprints of mineral systems in the regolith at regional to continental scales

ABSTRACT Understanding the character of Australia's extensive regolith cover is crucial to the continuing success of mineral exploration. We hypothesise that the regolith contains geochemical fingerprints of processes related to the development and preservation of mineral systems at a range of scales. We test this hypothesis by analysing the composition of surface sediments within greenfield regional-scale (southern Thomson Orogen) and continental-scale (Australia) study areas. In the southern Thomson Orogen area, the first principal component (PC1) derived in our study [Ca, Sr, Cu, Mg, Au and Mo at one end; rare earth elements (REEs) and Th at the other] is very similar to the empirical vector used by a local company (enrichment in Sr, Ca and Au concomitant with depletion in REEs) to successfully site exploration drill holes for Cu–Au mineralisation. Mapping of the spatial distribution of PC1 in the region reveals several areas of elevated values and possible mineralisation potential. One of the strongest targets in the PC1 map is located between Brewarrina and Bourke in northern New South Wales. Here, exploration drilling has intersected porphyry Cu–Au mineralisation with up to 1 wt% Cu, 0.1 g/t Au, and 717 ppm Zn. The analysis of a comparable geochemical dataset at the continental scale yields a compositionally similar PC1 (Ca, Sr, Mg, Cu, Au and Mo at one end; REEs and Th at the other) to that of the regional study. Mapping PC1 at the continental scale shows patterns that (1) are spatially compatible with the regional study and (2) reveal several geological regions of elevated values, possibly suggesting an enhanced potential for porphyry Cu–Au mineralisation. These include well-endowed mineral provinces such as the Curnamona and Capricorn regions, but also some greenfield regions such as the Albany-Fraser/western Eucla, western Murray and Eromanga geological regions. We conclude that the geochemical composition of Australia's regolith may hold critical information pertaining to mineralisation within/beneath it.
Creating a Knowledge Base of Biological Research Papers

Intelligent text-oriented tools for representing and searching the biological research literature are being developed, which combine object-oriented databases with artificial intelligence techniques to create a richly structured knowledge based of Materials and Methods sections of biological research papers. A knowledge model of experimental processes, biological and chemical substances, and analytical techniques is described, based on the representation techniques of taxonomic semantic nets and knowledge frames. Two approaches to populating the knowledge base with the contents of biological research papers are described: natural language processing and an interactive knowledge definition tool.
Effects of Promyelocytic Leukemia Protein on Virus-Host Balance

ABSTRACT The cellular promyelocytic leukemia protein (PML) associates with the proteins of several viruses and in some cases reduces viral propagation in cell culture. To examine the role of PML in vivo, we compared immune responses and virus loads of PML-deficient and control mice infected with lymphocytic choriomeningitis virus (LCMV) and vesicular stomatitis virus (VSV). PML−/− mice exhibited accelerated primary footpad swelling reactions to very-low-dose LCMV, higher swelling peaks upon high-dose inoculation, and higher viral loads in the early phase of systemic LCMV infection. T-cell-mediated hepatitis and consequent mortality upon infection with a hepatotropic LCMV strain required 10- to 100-times-lower inocula despite normal cytotoxic T-lymphocyte reactivity in PML−/− mice. Furthermore, PML deficiency rendered mice 10 times more susceptible to lethal immunopathology upon intracerebral LCMV inoculation. Accordingly, 10-times-lower VSV inocula elicited specific neutralizing-antibody responses, a replication-based effect not observed with inactivated virus or after immunization with recombinant VSV glycoprotein. These in vivo observations corroborated our results showing more virus production in PML−/− fibroblasts. Thus, PML is a contributor to innate immunity, defining host susceptibility to viral infections and to immunopathology.
Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii

Mutations at two loci, which cause an altered mobility of the flagella, affected the central pair microtubule complex of Chlamydomonas reinhardtii flagella. The mutations at both loci primarily affected the C1 microtubule of the complex. Three alleles at the PF16 locus affected the stability of the C1 microtubule in isolated axonemes. This phenotype has allowed us to determine that at least ten polypeptides of the central pair complex are unique to the C1 microtubule. The motility defect was correlated with the failure to assemble three of these ten polypeptides in vivo. The structural gene product of the PF16 locus was a polypeptide with molecular weight 57,000 as shown by analysis of five intragenic revertants and by analysis of axonemes from dikaryon rescue experiments. Three alleles at the PF6 locus affected the assembly of one of the two projections of the C1 microtubule and this projection was formed by at least three polypeptide components, which are a subset of polypeptides missing in isolated pf16 axonemes. No structural gene product has been identified for the PF6 locus. The gene product is probably not one of the identified projection constituents as shown by analysis of dikaryon rescue experiments. Chemical extraction of isolated wild-type axonemes suggests that at least seven polypeptide components are unique to the C2 microtubule.
Atomic Force Microscopy Characterization of Protein Fibrils Formed by the Amyloidogenic Region of the Bacterial Protein MinE on Mica and a Supported Lipid Bilayer

Amyloid fibrils play a crucial role in many human diseases and are found to function in a range of physiological processes from bacteria to human. They have also been gaining importance in nanotechnology applications. Understanding the mechanisms behind amyloid formation can help develop strategies towards the prevention of fibrillation processes or create new technological applications. It is thus essential to observe the structures of amyloids and their self-assembly processes at the nanometer-scale resolution under physiological conditions. In this work, we used highly force-sensitive frequency-modulation atomic force microscopy (FM-AFM) to characterize the fibril structures formed by the N-terminal domain of a bacterial division protein MinE in solution. The approach enables us to investigate the fibril morphology and protofibril organization over time progression and in response to changes in ionic strength, molecular crowding, and upon association with different substrate surfaces. In addition to comparison of the fibril structure and behavior of MinE1-31 under varying conditions, the study also broadens our understanding of the versatile behavior of amyloid-substrate surface interactions.
SAFER PATH: Security architecture using fragmented execution and replication for protection against trojaned hardware

Ensuring electronic components are free from Hardware Trojans is a very difficult task. Research suggests that even the best pre- and post-deployment detection mechanisms will not discover all malicious inclusions, nor prevent them from being activated. For economic reasons electronic components are used regardless of the possible presence of such Trojans. We developed the SAFER PATH architecture, which uses instruction and data fragmentation, program replication, and voting to create a computational system that is able to operate safely in the presence of active Hardware Trojans. We protect the integrity of the computation, the confidentiality of data being processed and ensure system availability. By combining a small Trusted Computing Base with Commercial-Off-The-Shelf processing elements, we are able to protect computation from the effects of arbitrary Hardware Trojans.
Plant Defense against Herbivorous Pests: Exploiting Resistance and Tolerance Traits for Sustainable Crop Protection

Interactions between plants and insect herbivores are important determinants of plant productivity in managed and natural vegetation. In response to attack, plants have evolved a range of defenses to reduce the threat of injury and loss of productivity. Crop losses from damage caused by arthropod pests can exceed 15% annually. Crop domestication and selection for improved yield and quality can alter the defensive capability of the crop, increasing reliance on artificial crop protection. Sustainable agriculture, however, depends on reduced chemical inputs. There is an urgent need, therefore, to identify plant defensive traits for crop improvement. Plant defense can be divided into resistance and tolerance strategies. Plant traits that confer herbivore resistance typically prevent or reduce herbivore damage through expression of traits that deter pests from settling, attaching to surfaces, feeding and reproducing, or that reduce palatability. Plant tolerance of herbivory involves expression of traits that limit the negative impact of herbivore damage on productivity and yield. Identifying the defensive traits expressed by plants to deter herbivores or limit herbivore damage, and understanding the underlying defense mechanisms, is crucial for crop scientists to exploit plant defensive traits in crop breeding. In this review, we assess the traits and mechanisms underpinning herbivore resistance and tolerance, and conclude that physical defense traits, plant vigor and herbivore-induced plant volatiles show considerable utility in pest control, along with mixed species crops. We highlight emerging approaches for accelerating the identification of plant defensive traits and facilitating their deployment to improve the future sustainability of crop protection.
What Homophones Say about Words

The number of potential meanings for a new word is astronomic. To make the word-learning problem tractable, one must restrict the hypothesis space. To do so, current word learning accounts often incorporate constraints about cognition or about the mature lexicon directly in the learning device. We are concerned with the convexity constraint, which holds that concepts (privileged sets of entities that we think of as “coherent”) do not have gaps (if A and B belong to a concept, so does any entity “between” A and B). To leverage from it a linguistic constraint, learning algorithms have percolated this constraint from concepts, to word forms: some algorithms rely on the possibility that word forms are associated with convex sets of objects. Yet this does have to be the case: homophones are word forms associated with two separate words and meanings. Two sets of experiments show that when evidence suggests that a novel label is associated with a disjoint (non-convex) set of objects, either a) because there is a gap in conceptual space between the learning exemplars for a given word or b) because of the intervention of other lexical items in that gap, adults prefer to postulate homophony, where a single word form is associated with two separate words and meanings, rather than inferring that the word could have a disjunctive, discontinuous meaning. These results about homophony must be integrated to current word learning algorithms. We conclude by arguing for a weaker specialization of word learning algorithms, which too often could miss important constraints by focusing on a restricted empirical basis (e.g., non-homophonous content words).