Miracle-dz/UniFAR-FTUs huggingface dataset:
Can we manage fisheries with the inherent uncertainty from eDNA?

Environmental (e)DNA, as a general approach in aquatic systems, seeks to connect the presence of species' genetic material in the water and hence to infer the species' physical presence. However, fisheries managers face making decisions with risk and uncertainty when eDNA indicates a fish is present but traditional methods fail to capture the fish. In comparison with traditional methods such as nets, electrofishing and piscicides, eDNA approaches have more sources of underlying error that could give rise to false positives. This has resulted in some managers to question whether eDNA can be used to make management decisions because there is no fish in hand. As a relatively new approach, the methods and techniques have quickly evolved to improve confidence in eDNA. By evaluating an eDNA based research programmes through the pattern of the eDNA signal, assay design, experimental design, quality assurance and quality control checks, data analyses and concurrent search for fish using traditional gears, the evidence for fish presence can be evaluated to build confidence in the eDNA approach. The benefits for fisheries management from adopting an eDNA approach are numerous but include cost effectiveness, broader geographic coverage of habitat occupancy, early detection of invasive species, non-lethal stock assessments, exploration of previously inaccessible aquatic environments and discovery of new species hidden beneath the water's surface. At a time when global freshwater and marine fisheries are facing growing threats from over-harvest, pollution and climate change, we anticipate that growing confidence in eDNA will overcome the inherent uncertainty of not having a fish in hand and will empower the informed management actions necessary to protect and restore our fisheries. This article is protected by copyright. All rights reserved.
A Virus-Infected, Reprogrammed Somatic Cell–Derived Tumor Cell (VIReST) Vaccination Regime Can Prevent Initiation and Progression of Pancreatic Cancer

Purpose: Pancreatic cancer remains one of the most lethal cancers, and late detection renders most tumors refractory to conventional therapies. Development of cancer prophylaxis may be the most realistic option for improving mortality associated with this disease. Here, we develop a novel individualized prophylactic and therapeutic vaccination regimen using induced pluripotent stem cells (iPSC), gene editing, and tumor-targeted replicating oncolytic viruses. Experimental Design: We created a Virus-Infected, Reprogrammed Somatic cell-derived Tumor cell (VIReST) regime. iPSCs from healthy cells were induced to pancreatic tumor cells using in situ gene editing via stable provision of KRasG12D and p53R172H tumor driver mutations. These cells were preinfected with oncolytic Adenovirus (AdV) as prime or Vaccinia virus (VV) as boost, to improve vaccine immunogenicity, prior to delivery of vaccines in a sequential regime to young KPC transgenic mice, genetically programmed to develop pancreatic cancer, to prevent and delay disease development. Results: Tumor cells preinfected with oncolytic AdV as prime or VV as boost were the best regime to induce tumor-specific immunity. iPSC-derived tumor cells were highly related in antigen repertoire to pancreatic cancer cells of KPC transgenic mice, suggesting that an individual's stem cells can provide an antigenically matched whole tumor cell vaccine. The VIReST vaccination primed tumor-specific T-cell responses, resulting in delayed disease emergence and progression and significantly prolonged survival of KPC transgenic mice. Importantly, this regime was well-tolerated and nontoxic. Conclusions: These results provide both proof of concept and a robust technology platform for the development of personalized prophylactic cancer vaccines to prevent pancreatic malignancies in at-risk individuals.
Targeting STAT3 and STAT5 in Tumor-Associated Immune Cells to Improve Immunotherapy

Oncogene-induced STAT3-activation is central to tumor progression by promoting cancer cell expression of pro-angiogenic and immunosuppressive factors. STAT3 is also activated in infiltrating immune cells including tumor-associated macrophages (TAM) amplifying immune suppression. Consequently, STAT3 is considered as a target for cancer therapy. However, its interplay with other STAT-family members or transcription factors such as NF-κB has to be considered in light of their concerted regulation of immune-related genes. Here, we discuss new attempts at re-educating immune suppressive tumor-associated macrophages towards a CD8 T cell supporting profile, with an emphasis on the role of STAT transcription factors on TAM functional programs. Recent clinical trials using JAK/STAT inhibitors highlighted the negative effects of these molecules on the maintenance and function of effector/memory T cells. Concerted regulation of STAT3 and STAT5 activation in CD8 T effector and memory cells has been shown to impact their tumor-specific responses including intra-tumor accumulation, long-term survival, cytotoxic activity and resistance toward tumor-derived immune suppression. Interestingly, as an escape mechanism, melanoma cells were reported to impede STAT5 nuclear translocation in both CD8 T cells and NK cells. Ours and others results will be discussed in the perspective of new developments in engineered T cell-based adoptive therapies to treat cancer patients.
Natural Antibacterial Reagents (Centella, Propolis, and Hinokitiol) Loaded into Poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] Composite Nanofibers for Biomedical Applications

Centella asiatica, propolis, and hinokitiol, as natural antibacterial reagents, were integrated into the poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] (PHBH) polymer to produce antibacterial wound dressings, using electrospinning process. The results showed that the fiber diameters and surface morphology of PHBH composite nanofibers were influenced by the addition of ethanol–centella (EC), methanol–centella (MC), ethanol–propolis (EP), and ethanol–hinokitiol (EH) at various ratios compared to pristine PHBH nanofibers. From FT-IR, the nanofibrous samples with higher contents of natural antibacterial substances showed the peaks of carboxylic acid, aromatic ring, and tropolone carbon ring from centella, propolis, and hinokitiol, respectively. Furthermore, the tensile strength of neat PHBH nanofibers was increased from 8.00 ± 0.71 MPa up to 16.35 ± 1.78 MPa by loading of propolis (EP) 7% into PHBH. X-ray analysis explained that the loading of propolis (EP) was also able to increase the crystallinity in PHBH composite nanofibers from 47.0% to 54.5%. The antibacterial results demonstrated that PHBH composite nanofibers containing natural antibacterial products were potent inhibitors against the growth of Escherichia coli and Staphylococcus aureus, amongst them hinokitiol and propolis proved to be the most effective. Additionally, the release studies displayed that centella and hinokitiol had faster release from PHBH composite nanofibers in comparison to propolis.
Media Use of Mothers, Media Use of Children, and Parent–Child Interaction Are Related to Behavioral Difficulties and Strengths of Children

The present study investigated the associations of media use of children, media use of mothers, and parent-child interactions with behavioral strengths and difficulties in children. Screen time of 553 2- to 9-year-old children and their mothers were indicated by the daily durations of their TV/games console/computer/mobile phone use. The amount of parent–child interaction was indicated by the frequencies of shared activities at home. Behavioral strengths and difficulties of children were investigated using the Strengths and Difficulties Questionnaire. Children whose mothers reported high screen times (>/= 5 h/day) were significantly more likely to show high screen times (>/= 2 h/day). High screen time of children was associated with more conduct problems, more symptoms of hyperactivity/inattention and less prosocial behavior. High screen time of mothers was associated with emotional problems, conduct problems, and symptoms of hyperactivity/inattention. In contrast, a higher frequency of parent–child interactions was associated with fewer conduct problems, fewer peer-relationship problems, and more prosocial behavior of children. Children might use the media behavior of their mothers as a role model for their own media use. Furthermore, the findings suggest that media use of children and mothers and parent–child interaction contribute independently to behavioral strengths and difficulties of children.
Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers.

BACKGROUND Ferroptosis, nonapoptotic cell death mediated by free radical reactions and driven by the oxidative degradation of lipids, is a therapeutic target because of its role in organ damage, including AKI. Ferroptosis-causing radicals that are targeted by ferroptosis suppressors have not been unequivocally identified. Because certain cytochrome P450 substrate drugs can prevent lipid peroxidation via obscure mechanisms, we evaluated their antiferroptotic potential and used them to identify ferroptosis-causing radicals. METHODS Using a cell-based assay, we screened cytochrome P450 substrate compounds to identify drugs with antiferroptotic activity and investigated the underlying mechanism. To evaluate radical-scavenging activity, we used electron paramagnetic resonance-spin trapping methods and a fluorescence probe for lipid radicals, NBD-Pen, that we had developed. We then assessed the therapeutic potency of these drugs in mouse models of cisplatin-induced AKI and LPS/galactosamine-induced liver injury. RESULTS We identified various US Food and Drug Administration-approved drugs and hormones that have antiferroptotic properties, including rifampicin, promethazine, omeprazole, indole-3-carbinol, carvedilol, propranolol, estradiol, and thyroid hormones. The antiferroptotic drug effects were closely associated with the scavenging of lipid peroxyl radicals but not significantly related to interactions with other radicals. The elevated lipid peroxyl radical levels were associated with ferroptosis onset, and known ferroptosis suppressors, such as ferrostatin-1, also functioned as lipid peroxyl radical scavengers. The drugs exerted antiferroptotic activities in various cell types, including tubules, podocytes, and renal fibroblasts. Moreover, in mice, the drugs ameliorated AKI and liver injury, with suppression of tissue lipid peroxidation and decreased cell death. CONCLUSIONS Although elevated lipid peroxyl radical levels can trigger ferroptosis onset, some drugs that scavenge lipid peroxyl radicals can help control ferroptosis-related disorders, including AKI.
The Anthropometric Measurements of Tibia

Anthropometry provides scientific method and technique for taking various measurements in different geographic regions and races. The Tibia itself is a complex anatomic unit so anthropometric study was devised on the same. In the present study 60 (26 right and 34 left) intact adult tibia were obtained from the bone bank of Anatomy Department of MGIMS, Sevagram. For this purpose a digital vernier caliper, osteometric board and measuring tape were used.
Comparison of 24‐hour urine and 24‐hour diet recall for estimating dietary sodium intake in populations: A systematic review and meta‐analysis

This systematic literature review and meta‐analysis examined whether 24‐hour diet recall is a valid way to measure mean population sodium intake compared with the gold standard 24‐hour urinary assessment. The authors searched electronic databases MEDLINE, Embase, and Scopus using pre‐defined terms. Studies were eligible for inclusion if they assessed adult humans in free‐living settings, and if they included group means for 24‐hour diet recall and 24‐hour urinary collection of sodium intake in the same participants. Studies that included populations with an active disease state that might interfere with normal sodium metabolism were excluded. Results of 28 studies are included in the meta‐analysis. Overall, 24‐hour diet recall underestimated population mean sodium intake by an average of 607 mg per day compared to the 24‐hour urine collection. The difference between measures from 24‐hour urine and 24‐hour diet recall was smaller in studies conducted in high‐income countries, in studies where multiple‐pass methods of 24‐hour diet recall were reported and where urine was validated for completeness. Higher quality studies also reported smaller differences between measures than lower quality studies. Monitoring of population sodium intake with 24‐hour urinary excretion remains the most accurate method of assessment. Twenty‐four‐hour diet recall tends to underestimate intake, although high‐quality 24‐hour diet recall improves accuracy, and may be used if 24‐hour urine is not feasible.
Methyljasmonate Elicitation Increases Terpenoid Indole Alkaloid Accumulation in Rhazya stricta Hairy Root Cultures

Methyl jasmonate is capable of initiating or improving the biosynthesis of secondary metabolites in plants and therefore has opened up a concept for the biosynthesis of valuable constituents. In this study, the effect of different doses of methyl jasmonate (MeJA) elicitation on the accumulation of terpenoid indole alkaloids (TIAs) in the hairy root cultures of the medicinal plant, Rhazya stricta throughout a time course (one-seven days) was investigated. Gas chromatography-mass spectrometry (GC-MS) analyses were carried out for targeted ten major non-polar alkaloids. Furthermore, overall alterations in metabolite contents in elicited and control cultures were investigated applying proton nuclear magnetic resonance (1H NMR) spectroscopy. Methyl jasmonate caused dosage- and time course-dependent significant rise in the accumulation of TIAs as determined by GC-MS. The contents of seven alkaloids including eburenine, quebrachamine, fluorocarpamine, pleiocarpamine, tubotaiwine, tetrahydroalstonine, and ajmalicine increased compared to non-elicited cultures. However, MeJA-elicitation did not induce the accumulation of vincanine, yohimbine (isomer II), and vallesiachotamine. Furthermore, principal component analysis (PCA) of 1H NMR metabolic profiles revealed a discrimination between elicited hairy roots and control cultures with significant increase in total vindoline-type alkaloid content and elevated levels of organic and amino acids. In addition, elicited and control samples had different sugar and fatty acid profiles, suggesting that MeJA also influences the primary metabolism of R. stricta hairy roots. It is evident that methyl jasmonate is applicable for elevating alkaloid accumulation in “hairy root” organ cultures of R. strica.
Limb Salvage Operations for Patients with Malignant Bone Tumors in the Extremities

Neoadjuvant chemotherapy, modem imaging analyses, and developments in surgical techniques in recent decades have enabled limb salvage surgery to provide well-functioning, tumor-free, painless limbs through excision of the tumor with an adequate margin and proper reconstruction. With proper selection, patients are expected to have mostly intact body balance, a simple rehabilitation process, and an intact body image, but not at the expense of local recurrence and survival. Currently the indications of such a limb salvage procedure have been expanded to other non-malignant tumorous conditions in addition to malignant juxta-articular bone tumors in the extremities. Wide resection of a tumor can result in massive bone and soft-tissue defects. Currently, many options for reconstruction with limb salvage procedures are available, including resection arthrodesis, various bone graft techniques (vascularized or conventional autografts, allografts, or allograft/endoprosthesis composites), Ilizarov leg lengthening, rotationplasty, reconstruction with custom-made oncological endoprostheses, etc. These have resulted in expected successes, while simultaneously producing biological or prosthesis-related complications due to their unique advantages and disadvantages. The choice of an option requires careful individual considerations, including imaging radiological staging, pathological staging, and the clinical status.
Assessment of Nutritional Status and Dietary Intake of Pregnant Women in Rural Area of Vhembe District, Limpopo Province

ABSTRACT Poor consumption of balanced diet could result in poor weight gain during pregnancy increasing the risk of premature delivery, low birth weight, and birth defects. To assess the nutritional status and dietary intake of pregnant women in rural areas of Vhembe district. A cross-sectional descriptive study was carried out among 240pregnant women who were selected conveniently from 16 clinics in Vhembe District. An interviewer-administered questionnaire was used for data collection. Anthropometric measurements were measured following standard techniques. Data on dietary intake were collected using Food Frequency Questionnaire (FFQ). Permission and clearance were obtained and participant’s rights were respected. Majority (78%) had secondary educational level while 19.5% had tertiary educational level. The mean energy and carbohydrate intake was 2248 Kcal and 372.1 g, respectively. Prevalence of underweight, overweight and obese using BMI was 16.3%, 24.2%, and 8.7% respectively. Dietary intake of the study participants showed that the intake of energy, fats, carbohydrates and vitamin C met the Recommended Dietary Allowance (RDA) values. The mean intake of protein was 30.2 ± 18.2 g. However, micronutrients like zinc, iron, magnesium, calcium including Vitamin A, B1 and B2 did not meet the RDA values in the current study. Despite government’s programs to ensure adequate consumption of micronutrients and proper weight gain during pregnancy, malnutrition and insufficient consumption of micronutrients remains a major public health problem in South Africa. Improving nutritional status during pregnancy should follow an integrated approach tackling both malnutrition and micronutrient deficiencies at the same time considering the behavioral approach which will improve child survival and maternal health.
FoxO3a as a Positive Prognostic Marker and a Therapeutic Target in Tamoxifen-Resistant Breast Cancer

Background: Resistance to endocrine treatments is a major clinical challenge in the management of estrogen receptor positive breast cancers. Although multiple mechanisms leading to endocrine resistance have been proposed, the poor outcome of this subgroup of patients demands additional studies. Methods: FoxO3a involvement in the acquisition and reversion of tamoxifen resistance was assessed in vitro in three parental ER+ breast cancer cells, MCF-7, T47D and ZR-75-1, in the deriving Tamoxifen resistant models (TamR) and in Tet-inducible TamR/FoxO3a stable cell lines, by growth curves, PLA, siRNA, RT-PCR, Western blot, Immunofluorescence, Transmission Electron Microscopy, TUNEL, cell cycle, proteomics analyses and animal models. FoxO3a clinical relevance was validated in silico by Kaplan–Meier survival curves. Results: Here, we show that tamoxifen resistant breast cancer cells (TamR) express low FoxO3a levels. The hyperactive growth factors signaling, characterizing these cells, leads to FoxO3a hyper-phosphorylation and subsequent proteasomal degradation. FoxO3a re-expression by using TamR tetracycline inducible cells or by treating TamR with the anticonvulsant lamotrigine (LTG), restored the sensitivity to the antiestrogen and strongly reduced tumor mass in TamR-derived mouse xenografts. Proteomics data unveiled novel potential mediators of FoxO3a anti-proliferative and pro-apoptotic activity, while the Kaplan–Meier analysis showed that FoxO3a is predictive of a positive response to tamoxifen therapy in Luminal A breast cancer patients. Conclusions: Altogether, our data indicate that FoxO3a is a key target to be exploited in endocrine-resistant tumors. In this context, LTG, being able to induce FoxO3a, might represent a valid candidate in combination therapy to prevent resistance to tamoxifen in patients at risk.
Spectral Overlays for Reading Difficulties: Oculomotor Function and Reading Efficiency Among Children and Adolescents With Visual Stress

This study analyzed the effects of spectral overlays on ocular motility during reading among a clinical group of children and adolescents experiencing visual–perceptual distortions of text. We reviewed the records of 323 eye-hospital patients diagnosed with visual stress and divided this participant sample into two age-based cohorts: children (n = 184; Mean [M] age = 10.1, standard deviation [SD] = 1.3 years) and adolescents (n = 139; M age = 14.6, SD = 1.5 years). We used a Visagraph III Eye-Movement Recording System to record ocular motor efficiency while reading with and without spectral overlays, and we examined the following parameters: (a) Fixations, (b) Regressions, (c) Span of Recognition, (d) Reading Rate, (e) Relative Efficiency, and (f) Comprehension. Our results showed that using one or some combination of 10 participant-selected spectral overlays immediately and significantly (p < .001) reduced the number of Fixations and Regressions per 100 words, while there were significant (p < .001) gains in positive factors such as Span of Recognition, Reading Rate, Relative Efficiency, and Comprehension. Our findings indicate that spectral filtering can be an effective tool for helping many young patients who experience visual–perceptual distortions while reading. Future expanded research employing eye-tracking technology is clearly needed.
Observer-Based Event-Triggered Adaptive Fuzzy Control for Leader-Following Consensus of Nonlinear Strict-Feedback Systems

In this article, the leader-following consensus problem via the event-triggered control technique is studied for the nonlinear strict-feedback systems with unmeasurable states. The follower’s nonlinear dynamics is approximated using the fuzzy-logic systems, and the fuzzy weights are updated in a nonperiodic manner. By introducing a fuzzy state observer to reconstruct the system states, an observer-based event-triggered adaptive fuzzy control and a novel event-triggered condition are designed, simultaneously. In addition, the nonzero positive lower bound on interevent intervals is presented to avoid the Zeno behavior. It is proved via an extension of the Lyapunov approach that ultimately bounded control is achieved for the leader-following consensus of the considered multiagent systems. One remarkable advantage of the proposed control protocol is that the control law and fuzzy weights are updated only when the event-triggered condition is violated, which can greatly decrease the data transmission and communication resource. The simulation results are provided to show the effectiveness of the proposed control strategy and the theoretical analysis.