Miracle-dz/UniFAR-FTUs huggingface dataset:
Altered Protein Function Caused by AMD-associated Variant rs704 Links Vitronectin to Disease Pathology

Purpose Vitronectin, a cell adhesion and spreading factor, is suspected to play a role in the pathogenesis of age-related macular degeneration (AMD), as it is a major component of AMD-specific extracellular deposits (e.g., soft drusen, subretinal drusenoid deposits). The present study addressed the impact of AMD-associated non-synonymous variant rs704 in the vitronectin-encoding gene VTN on vitronectin functionality. Methods Effects of rs704 on vitronectin expression and processing were analyzed by semi-quantitative sequencing of VTN transcripts from retinal pigment epithelium (RPE) cells generated from human induced pluripotent stem cells (hiPSCs) and from human neural retina, as well as by western blot analyses on heterologously expressed vitronectin isoforms. Binding of vitronectin isoforms to retinal and endothelial cells was analyzed by western blot. Immunofluorescence staining followed extracellular matrix (ECM) deposition in cultured RPE cells heterologously expressing the vitronectin isoforms. Adhesion of fluorescently labeled RPE or endothelial cells in dependence of recombinant vitronectin or vitronectin-containing ECM was investigated fluorometrically or microscopically. Tube formation and migration assays addressed effects of vitronectin on angiogenesis-related processes. Results Variant rs704 affected expression, secretion, and processing but not oligomerization of vitronectin. Cell binding and influence on RPE-mediated ECM deposition differed between AMD-risk-associated and non-AMD-risk-associated protein isoforms. Finally, vitronectin affected adhesion and endothelial tube formation. Conclusions The AMD-risk-associated vitronectin isoform exhibits increased expression and altered functionality in cellular processes related to the sub-RPE aspects of AMD pathology. Although further research is required to address the subretinal disease aspects, this initial study supports an involvement of vitronectin in AMD pathogenesis.
The isolation of two new compounds from Valeriana jatamansi

Abstract A new norsesquiterpene, wilfordonol E (1), and a new lignan, dipsalignan E (3), together with a known norsesquiterpene and eleven known lignans were isolated from the roots and rhizomes of Valeriana jatamansi. The structures of new compounds were determined by their NMR and HR-ESI-MS spectra. Additionally, some compounds were evaluated for their anti-influenza A virus effects. Graphical Abstract
Alcohol and Betting Radio Advertising in Spain. A Comparative Analysis of the Minor’s Protection Time Slot from a Media Responsibility Perspective

This research analyzes the presence of advertising and radio mentions of alcoholic beverages and sports betting, two products that represent harmful behaviors for the audiences’ health. To do this, a quantitative content analysis was undertaken for all of the programming of the three most listened radio stations in Spain (Cadena Ser, COPE, and Onda Cero) throughout 2017, obtaining a total corpus of 2848 radio messages distributed as follows: 266 radio spots and 2582 radio mentions. The messages were also analyzed according to the broadcasting schedules (protected time-slot or non-protected time-slot). The results showed that advertising and sports betting mentions were more present in the regular programming of the three stations (n = 2304), with mentions (n = 2582) being more numerous than advertising spots (n = 544). Moreover, it is evident that in practice, none of the radio stations respected the protected time slots since the majority of the mentions and spots of high alcoholic beverages and sports bets were verified between 6:00–21:59 (n = 2522). These results show the prevailing need for greater control over this type of content by public entities, demonstrating a significant lack of regulation by the media’s self-control mechanisms.
Proton positions in and thermal behaviour of the phase 4 CaO · 3 Al2O3 · 3 H2O and its thermal decomposition to |(OCa4)2|[Al12O24]-SOD, determined by neutron/X-ray powder diffraction and IR spectroscopic investigations

The proton positions in the phase 4 CaO · 3 Al2O3 · 3 H2O (C4A3H3) were determined by neutron diffraction exper iments at room temperature and 5 K. Two crystallographically distinct proton sites, 16g and 8f in space group Abma, were located from the evaluation of difference Fourier maps. Additionally, IR spectroscopic measurements were carried out to further characterise the observed OH groups. On the basis of this knowledge and due to topo logical considerations, we propose to write C4A3H3 according to the IUPAC nomenclature for microporous mate rials as an interrupted framework host structure with the formula |Ca8(OH)4|2 [Al4[4][1;4]Al8[4][1;3]. O20[2](OH)8[1]]2h{3; 4-ring}p{0[4668]} (Abma) – -SOD. X-ray diffraction experiments in the temperature range from 28 K to 973 K give an insight into the thermal behaviour of the phase C4A3H3, and its topotac tic condensation to |Ca8O2|[Al12[4][1;4]O24[2]]h{3; 4-ring}p · {0[4668]} (I-43m)-SOD (C4A3) + 6 H2O. The questioned fractional coordinates of the framework oxygens in the decomposition product C4A3, as described in the first structure de termination, were corrected by a structure refinement using the Rietveld method. It is shown unambiguously that |(OCa4)2| · [Al12O24]-SOD has a sodalite-type structure.
Pre-Clinical Investigation of Liquid Paclitaxel for Local Drug Delivery: A Pilot Study

The purpose of this pilot study was to investigate the feasibility of a perfusion catheter to deliver liquid paclitaxel into arterial segments. A clinically relevant rabbit ilio-femoral injury model was utilized to determine the impact of liquid paclitaxel delivered locally into the vessel wall using a perfusion catheter at 1 h to 14 days. Treatment by two clinically available forms of liquid paclitaxel, a solvent-based (sb) versus an albumin-bound (nab), along with a control (uncoated balloons), were investigated. Pharmacokinetic results demonstrated an increase in the retention of the sb-paclitaxel versus the nab-paclitaxel at 1 h; however, no other differences were observed at days one, three, and seven. Histological findings at 14 days showed significantly less neointimal area in the sb-paclitaxel treated arteries as compared with the nab-paclitaxel and the uncoated balloon-treated arteries. Additionally, percent area stenosis was significantly less in the sb-paclitaxel group. These results support the concept of local liquid delivery of paclitaxel into the arterial segments.
New Formulation of a Methylseleno-Aspirin Analog with Anticancer Activity Towards Colon Cancer

Aspirin (ASA) has attracted wide interest of numerous scientists worldwide thanks to its chemopreventive and chemotherapeutic effects, particularly in colorectal cancer (CRC). Incorporation of selenium (Se) atom into ASA has greatly increased their anti-tumoral efficacy in CRC compared with the organic counterparts without the Se functionality, such as the promising antitumoral methylseleno-ASA analog (1a). Nevertheless, the efficacy of compound 1a in cancer cells is compromised due to its poor solubility and volatile nature. Thus, 1a has been formulated with native α-, β- and γ-cyclodextrin (CD), a modified β-CD (hydroxypropyl β-CD, HP-β-CD) and Pluronic F127, all of them non-toxic, biodegradable and FDA approved. Water solubility of 1a is enhanced with β- and HP- β-CDs and Pluronic F127. Compound 1a forms inclusion complexes with the CDs and was incorporated in the hydrophobic core of the F127 micelles. Herein, we evaluated the cytotoxic potential of 1a, alone or formulated with β- and HP- β-CDs or Pluronic F127, against CRC cells. Remarkably, 1a formulations demonstrated more sustained antitumoral activity toward CRC cells. Hence, β-CD, HP-β-CD and Pluronic F127 might be excellent vehicles to improve pharmacological properties of organoselenium compounds with solubility issues and volatile nature.
A review on recent advances in plasma-activated water for food safety: current applications and future trends

Abstract Plasma-activated water (PAW), the water or solutions treated with atmospheric cold plasma, is an eco-friendly technique with minimal changes in food products, making it a befitting alternative to traditional disinfection methods. Due to its potential microbicidal properties, PAW has been receiving increasing attention for applications in the food, agricultural, and biomedical fields. In this article, we aimed at presenting an overview of recent studies on the generation methods, physicochemical properties, and antimicrobial activity of PAW, as well as its application in the food industry. Specific areas were well discussed including microbial decontamination of food products, reduction of pesticide residues, meat curing, sprouts production, and disinfection of food contact materials. In addition, the factors influencing PAW efficiency were also well illustrated in detail, such as discharge parameters, types and amounts of microorganisms, characteristics of the liquid solution and food products, and treatment time. Moreover, the strategies to improve the efficacy of PAW were also presented in combination with other technologies. Furthermore, the salient drawbacks of this technology were discussed and the important areas for future research were also highlighted. Overall, the present review provides important insights for the application of PAW in the food industry.
Hybrid Transition Metal Dichalcogenide/Graphene Microspheres for Hydrogen Evolution Reaction

A peculiar 3D graphene-based architecture, i.e., partial reduced-Graphene Oxide Aerogel Microspheres (prGOAM), having a dandelion-like morphology with divergent microchannels to implement innovative electrocatalysts for the hydrogen evolution reaction (HER) is investigated in this paper. prGOAM was used as a scaffold to incorporate exfoliated transition metals dichalcogenide (TMDC) nanosheets, and the final hybrid materials have been tested for HER and photo-enhanced HER. The aim was to create a hybrid material where electronic contacts among the two pristine materials are established in a 3D architecture, which might increase the final HER activity while maintaining accessible the TMDC catalytic sites. The adopted bottom-up approach, based on combining electrospraying with freeze-casting techniques, successfully provides a route to prepare TMDC/prGOAM hybrid systems where the dandelion-like morphology is retained. Interestingly, the microspherical morphology is also maintained in the tested electrode and after the electrocatalytic experiments, as demonstrated by scanning electron microscopy images. Comparing the HER activity of the TMDC/prGOAM hybrid systems with that of TMDC/partially reduced-Graphene Oxide (prGO) and TMDC/Vulcan was evidenced in the role of the divergent microchannels present in the 3D architecture. HER photoelectron catalytic (PEC) tests have been carried out and demonstrated an interesting increase in HER performance.
Role of Thromboelastography as an Early Predictor of Disseminated Intravascular Coagulation in Patients with Septic Shock

(1) Background: The currently proposed criteria for diagnosing overt disseminated intravascular coagulation (DIC) are not suitable for early detection of DIC. Thromboelastography (TEG) rapidly provides a comprehensive assessment of the entire coagulation process and is helpful as a guide for correcting consumptive coagulopathy in sepsis-induced DIC. This study aimed to investigate the role of TEG in the prediction of DIC in patients with septic shock. (2) Methods: TEG was conducted prospectively in 1294 patients with septic shock at the emergency department (ED) between January 2016 and December 2019. After exclusion of 405 patients with “do not attempt resuscitation” orders, those refusing enrollment, and those developing septic shock after ED presentation, 889 patients were included. DIC was defined as an International Society on Thrombosis and Hemostasis score ≥ 5 points within 24 h. (3) Results: Of the 889 patients with septic shock (mean age 65.6 ± 12.7 years, 58.6% male), 158 (17.8%) developed DIC. TEG values, except lysis after 30 min, were significantly different between the DIC and non-DIC groups. Among the TEG values, the maximal amplitude (MA) had the highest discriminating power for DIC, with an area under the curve of 0.814. An MA < 60 indicated DIC with 79% sensitivity, 73% specificity, and 94% negative predictive value. Based on multivariable analysis, MA < 60 was an independent predictor of DIC (odds ratio 5.616 (95% confidence interval: 3.213–9.818)). (4) Conclusions: In patients with septic shock, the MA value in TEG could be a valuable tool for early prediction of DIC.
Impact of periodontitis on the Oral Health Impact Profile: A systematic review and meta-analysis.

BACKGROUND Periodontitis, being a chronic and multifactorial disease, affects oral health, and consequently, the patient's quality of life (QoL). The assessment of the oral health-related quality of life (OHRQoL) is possible with the Oral Health Impact Profile-14 (OHIP-14) questionnaire comprising 7 subdomains: functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability, and handicap. OBJECTIVES The aim of this study was to conduct a systematic review of cross-sectional or case-control studies concerning the impact of periodontitis on QoL measured with OHIP-14. The outcomes of the studies were subjected to a meta-analysis. MATERIAL AND METHODS On the basis of a survey of databases (MEDLINE, Scopus, Google Scholar, and Polish Medical Bibliography - PBL), 1,346 titles related thematically to the impact of periodontitis on QoL were obtained and analyzed. Ten studies were considered eligible for evaluation (8 cross-sectional ones and 2 case-control ones). RESULTS All studies indicated a significant influence of periodontitis on the deterioration of the OHIP-14 values. This relationship was shown to be directly modified in proportion to the degree of the advancement of the periodontal disease and to the extent of periodontal tissue damage. Our own meta-analysis confirmed the correlation between the prevalence of periodontitis and increased OHIP-14 scores with a cumulative odds ratio (OR) of 1.33, demonstrated a moderately significant deterioration of the OHIP-14 scores by 4.2 points in the group with periodontitis as compared to the control group, and assessed the probability of OHIP-14 deterioration to be 3.5 times greater in severe periodontitis. CONCLUSIONS The impact of periodontitis on the deterioration of OHRQoL is quite clearly explained by the clinical symptoms of periodontitis. According to patients, the most important problems that periodontitis may cause include psychological discomfort, stress, problems in interpersonal relations, or even difficulties in daily activities. This indicates the need for more of a holistic approach in planning the goals of the periodontal therapy, taking into account the psychological and social aspects of the patient's perception of the disease.
Premature Birth is an Independent Risk Factor for Early Adiposity Rebound: Longitudinal Analysis of BMI Data from Birth to 7 Years

Adiposity rebound (AR) refers to the second rise of the body mass index (BMI) curve that usually occurs physiologically between five and seven years of age. AR timing has a great impact on patients’ health, since early adiposity rebound (EAR) is associated with the development of metabolic syndrome later in life. We aimed to investigate the prevalence of EAR in a cohort of inborn preterm infants admitted to the Neonatal Intensive Care Section of the Policlinico University Hospital of Bari, Italy. Secondarily, we assessed whether some determinants such as (1) gender; (2) delivery mode; (3) birth weight and classification into small, normal, or large for gestational age; (4) type of feeding; (5) breastfeeding duration; (6) timing of introduction of solid food; (7) parental education; and (8) parental pre-pregnancy body mass index (BMI) influenced EAR in this cohort. The tertiary aim was to evaluate the prevalence of obesity or being overweight at seven years of age in children according to early versus timely AR. This is a prospective, population-based longitudinal study conducted at the Neonatal Intensive Care Section of the Policlinico University Hospital of Bari, Italy. Inborn preterm infants admitted to the neonatal ward between 2009 and 2011 were eligible. Enrolled preterm infants were evaluated at birth and at 1, 3, 6, 9, 12, 15, 18, and 24 months and 3, 4, 5, 6, and 7 years of age. Weight and height data were analyzed, and BMI was calculated. AR was assessed in the growth trajectory in a body mass index (BMI) plot. Of the 250 preterm newborns included, 100 completed the seven-year follow-up and entered the final analysis, 138 were lost during the seven-year follow-up, and in 12 cases parents withdrew over the course of the study. The prevalence of EAR in our cohort of preterm newborns was 54% at seven years of age. Early adiposity rebound was associated with being large for gestational age (LGA) at birth. No other factors were associated with EAR. Early adiposity rebounders had a significantly higher BMI at seven years compared to children with timely AR (17.2 ± 2.7 vs. 15.6 ± 2.05, p = 0.021). No significant differences were found in the prevalence of obesity or being overweight at seven years of age in children with early or timely AR (29% vs. 14%, p = 0.202). Ex-preterm infants have an increased risk of EAR. Since EAR may lead to long-term detrimental health effects with the onset of various chronic diseases (e.g., obesity, metabolic syndrome, etc.), healthcare providers should be prepared to counteract its occurrence, especially in delicate sub-populations of infants.
Evaluating Health Literacy among Adolescent and Young Adult Pregnant Women from a Low-Income Area of Northeast Brazil

Adequate health literacy is important for strong health outcomes during pregnancy, particularly among mothers with high risk of adverse outcomes related to pregnancy and childbirth. Understanding the health literacy of young pregnant women in low-income settings could support strategies to reduce adverse outcomes in this population. This exploratory study assessed the health literacy of young pregnant adolescents and young adults from a rural area in Northeast Brazil and associated factors such as socioeconomic conditions, adequacy of prenatal care, and social support from family and friends. In this cross-sectional study, 41 pregnant adolescents (13–18 years) and 45 pregnant adults (23–28 years) from the Rio Grande do Norte state, Brazil, were assessed regarding health literacy through the Short Assessment of Health Literacy for Portuguese-Speaking Adults (SAHLPA, score from 0–18, inadequate if <15). Income sufficiency, self-perceived school performance, compliance with recommendations for adequate prenatal care, and social support were also assessed. A linear regression analysis was conducted to evaluate the variables associated with the SAHLPA score. Ninety-five percent of the adolescents and 53.3% of the adults (p < 0.001) presented inadequate health literacy. Adolescent age (β − 3.5, p < 0.001), poorer self-perceived school performance (β − 2.8, p < 0.001), and insufficient income for basic needs (β − 2.8, p = 0.014) were associated with worse SAHLPA scores. Adolescent mothers have higher rates of inadequate health literacy in this population. Policies are needed to improve access to health information for young populations from rural low-income areas.
Officer and Organizational Correlates With Police Interventions in Domestic Violence in China

Although domestic violence has long been identified as a serious social problem in China, little is known about police officers’ attitudinal and behavioral tendencies toward such incidents. Drawing upon survey data collected from police officers in two Chinese provinces, this study assesses whether officer and organizational factors are correlated to police inaction and intervention in resolving family violence. More than a quarter of Chinese police officers often and sometime did not take any action when responding to domestic violence. Chinese officers favored most the least punitive approaches of mediation and separation, with the most punitive actions, written warning and criminal sanction as the least preferred interventions. We found that Chinese officers with low levels of knowledge about the domestic violence law, higher degrees of tolerance of violence and less supportive attitudes toward an active police role in handling domestic violence are less willing to take any action against the offenders. Chinese police officers who perceived stronger supervisory support and expressed better knowledge about China’s new domestic violence law are more likely to intervene in domestic violence, whereas police officers who expressed greater degrees of tolerance of violence and believed in gender equality in society are less inclined to intervene. Policy makers and police administrators ought to pay greater attention to frontline supervisors’ attitudes and behavior toward proper responses to family violence. If active intervention is preferred, then measures and programs should be put into place to improve police officers’ legal knowledge and communication and problem-solving skills pertaining to conflict resolution.
Multifunctional Adhesives on the Eggs of the Leaf Insect Phyllium philippinicum (Phasmatodea: Phylliidae): Solvent Influence and Biomimetic Implications

Leaf insects (Phylliidae) are well-camouflaged terrestrial herbivores. They imitate leaves of plants almost perfectly and even their eggs resemble seeds—visually and regarding to dispersal mechanisms. The eggs of the leaf insect Phyllium philippinicum utilize an adhesive system with a combination of glue, which can be reversibly activated through water contact and a water-responding framework of reinforcing fibers that facilitates their adjustment to substrate asperities and real contact area enhancement. So far, the chemical composition of this glue remains unknown. To evaluate functional aspects of the glue–solvent interaction, we tested the effects of a broad array of chemical solvents on the glue activation and measured corresponding adhesive forces. Based on these experiments, our results let us assume a proteinaceous nature of the glue with different functional chemical subunits, which enable bonding of the glue to both the surface of the egg and the unpredictable substrate. Some chemicals inhibited adhesion, but the deactivation was always reversible by water-contact and in some cases yielded even higher adhesive forces. The combination of glue and fibers also enables retaining the adhesive on the egg, even if detached from the egg’s surface. The gained insights into this versatile bioadhesive system could hereafter inspire further biomimetic adhesives.