Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of cerebrovascular reactivity recovery following high‐intensity interval training and moderate‐intensity continuous training

A common inclusion criterion when assessing cerebrovascular (CVR) metrics is for individuals to abstain from exercise for 12–24 hr prior to data collections. While several studies have examined CVR during exercise, the literature describing CVR throughout post‐exercise recovery is sparse. The current investigation examined CVR measurements in nine participants (seven male) before and for 8 hr following three conditions: 45‐min moderate‐continuous exercise (at ~50% heart‐rate reserve), 25‐min high‐intensity intervals (ten, one‐minute intervals at ~85% heart‐rate reserve), and a control day (30‐min quiet rest). The hypercapnic (40–60 mmHg) and hypocapnic (25–40 mmHg) slopes were assessed via a modified rebreathing technique and controlled stepwise hyperventilation, respectively. All testing was initiated at 8:00a.m. with transcranial Doppler ultrasound measurements to index cerebral blood velocity performed prior to the condition (pre) with serial follow‐ups at zero, one, two, four, six, and eight hours within the middle and posterior cerebral artery (MCA, PCA). Absolute and relative MCA and PCA hypercapnic slopes were attenuated following high‐intensity intervals at hours zero and one (all p < .02). No alterations were observed in either hypocapnic or hypercapnic slopes following the control or moderate‐continuous exercise (all p > .13), aside from a reduced relative hypercapnic MCA slope at hours zero and one following moderate‐continuous exercise (all p < .005). The current findings indicate the common inclusion criteria of a 12–24 hr time restriction on exercise can be reduced to two hours when performing CVR measures. Furthermore, the consistent nature of the CVR indices throughout the control day indicate reproducible testing sessions can be made between 8:00a.m. and 7:00p.m.
A study on the glycaemic balance in streptozotocin‐diabetic rats treated with an aqueous extract of Ficus carica (fig tree) leaves

The hypoglycaemic effect of an aqueous extract of Ficus carica leaves was studied in streptozotocin‐diabetic rats. The extract induced a significant hypoglycaemic effect after either oral‐ or intraperitoneal (i.p.) administration. Body weight loss was prevented in treated diabetic rats and the survival index was significantly affected by plasma insulin levels. Results show that Ficus carica aqueous extract has a clear hypoglycaemic activity in treated versus non‐treated diabetic rats. The mechanism involved in such an effect is not elucidated.
Aberrant expression of WWOX and its association with cancer stem cell biomarker expression.

BACKGROUND Nanog and CD133 are biomarkers of cancer stem cells (CSCs) that regulate cancer progression. The WW domain-containing oxidoreductase (WWOX) is a tumor suppressor protein that can inhibit tumor cell proliferation. The purpose of this study was to investigate the expression and clinical significance of Nanog, CD133, and WWOX in infiltrating breast cancer (IBC). METHODS Expressions of Nanog, CD133, and WWOX in 204 IBC specimens and their corresponding control specimens were detected by immunohistochemistry. Patients' clinicopathologic and follow-up data were also collected. RESULTS The rates of positive expression of Nanog and CD133 were significantly higher in IBC specimens than in control specimens, and their expression was positively associated with tumor size, grade, and tumor stages, lymph node metastasis (LNM), and tumor-node-metastasis (TNM) stage. The rate of positive expression of WWOX was significantly lower in IBC specimens than in control specimens, and its expression was inversely associated with tumor size, grade, and tumor stages, LNM, and TNM stage. Patients whose specimens expressed Nanog, CD133, or HER2 had a reduced overall survival (OS) when compared with patients not expressing these proteins. However, patients whose specimens expressed WWOX, ER, or PR had an increased OS when compared with patients who did not show expression. Multivariate analysis demonstrated that expression of Nanog, CD133, WWOX, ER, and HER2, and the TNM stage were independent prognostic factors for IBC patients. CONCLUSIONS Therefore, Nanog, CD133, and WWOX should be considered as promising prognostic factors and therapeutic targets in IBC.
Preimaginal Developmental Biology of Diachasmimorpha tryoni (Cameron), a Parasitoid of the Mediterranean Fruit Fly

The objectives of this study were to identify the preimaginal development of instars of Diachasmimorpha tryoni (Cameron) (Hymenoptera: Braconidae: Opiinae), a larvo-pupal parasitoid of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and to define its thermal requirements. Development was followed daily and parasitoid stage and size were noted. Larval samples were observed under a scanning electron microscope. Developmental rate as a function of temperature was studied with the Sharpe-DeMichele 6-parameter biophysical model and thermal requirements (lower developmental threshold and thermal constant) calculated using a linear regression. The duration of parasitoid development at seven temperatures ranged from 16.52 ± 0.96 days at 28 °C to 57.62 ± 1.85 days at 15 °C (males) and from 18.00 ± 0.79 days at 28 °C to 63.60 ± 1.52 days at 15 °C (females). Male development always took shorter than female development. No parasitoids emerged at 29 and 30 °C, which seems to be close to maximal temperature. The development required 322.6 ± 17.6 degree-days above a thermal threshold of 9.19 °C (both sexes cumulated). These data are compared with those of the host fruit fly, and the suitability of D. tryoni in controlling the Mediterranean fruit fly in Reunion Island is discussed. RésuméLes objectifs de cette étude étaient d’identifier les Stades de développement préimaginal de Diachasmimorpha tryoni (Cameron) (Hymenoptera: Braconidae: Opiinae), un parasitoïde larvopupal de la mouche méditerranéenne des fruits, Ceratitis capitata (Wiedemann), et de caractériser les exigences thermiques de cette espèce. Le développement a été suivi chaque jour et le Stade de développement ainsi que la taille du parasitoïde notés. Les échantillons larvaires ont été observés au Microscope Electroniqe à Balayage. Le taux de développement en fonction de la température a été étudié grâce au modèle biophysique à 6 paramètre de Sharpe-DeMichele. Les exigences thermiques (température seuil minimale de développement et constante thermique) ont été calculées en utilisant une régression linéaire. La durée du développement préimaginal du parasitoïde à sept températures constantes différentes a varié de 16,52 ± 0,96 jours à 28 °C à 57,62 ± 1,85 jours à 15 °C (pour les mâles) et de 18,00 ± 0,79 jours à 28 °C à 63,60 ± 1,52 jours à 15 °C (pour les femelles). Le développement des mâles a toujours été plus court que celui des femelles. Aucun parasitoïde n’a émergé à 29 et 30 °C, qui semble donc être proche de la température maximale de développement. Le développement a nécessité 322,6 ± 17,6 degrés-jours au-dessus d’une température seuil minimale de 9,19 °C (mâles et femelles cumulés). Ces données sont comparées avec celle du Tephritidae hôte, et la capacité de cette espèce à contrôler les populations de son hôte à la Réunion est discutée.
Management of odontogenic sinusitis: multidisciplinary consensus statement

Odontogenic sinusitis (ODS) can present a therapeutic dilemma because multiple treatment strategies have been reported. ODS review articles have been published, but they have lacked multidisciplinary collaboration and an evidence‐based methodology. The purpose of this article was to perform an evidence‐based review of ODS management options, and develop a multidisciplinary consensus statement on ODS management options.
Lipid classes, fatty acids, and tocopherols of leaves of six edible plant species

Young leaves of sir plants, namely Tamarindus indica (tamarind), Oxalis corniculata (Indian sorrel), Rumex vesicarius (bladder dock), Alternanthera sessilis, Trigonella foenumgraecum (fenugreek), and Talinum triangulare (Ceylon spinach), yielded, on extraction with chloroform-methanol 1.16, 1.47, 0.47, 0.54, 1.50, and 2.42% lipids (dry weight), respectively. Silica gel column chromatography yielded 37.0-80.5% neutral lipids (NL), 21.7-44.7% glycolipids (GL), and 1.2-26.5% phospholipids (PL)
Living donor liver transplants for sick recipients during COVID-19 pandemic—An experience from a tertiary center in India

The recent outbreak of COVID-19 has brought elective surgeries including liver transplantation to a standstill. The concerns in Living Donor Liver Transplant (LDLT) were that immunosuppressed recipients and healthy donors would be exposed to nosocomial SARS- CoV-2 infection.1 However, as patients began to suffer and die, Liver Transplant Society of India (LTSI) revised its guidelines 2 and allowed LDLT for those who were very sick, or had just recovered from a life threatening decompensation (high MELD/ CTP score) or had malignancy. Over 90 % of transplants in India are from live donors as we have a very low donation rate.
Call 911: Lower Ambulance Utilization Among Young Adults, Especially Women, with Stroke

ABSTRACT: Background: Delayed presentation to the emergency department influences acute stroke care and can result in worse outcomes. Despite public health messaging, many young adults consider stroke as a disease of older people. We determined the differences in ambulance utilization and delays to hospital presentation between women and men as well as younger (18–44 years) versus older (≥45 years) patients with stroke. Methods: We conducted a population-based retrospective study using national administrative health data from the Canadian Institute of Health Information databases and examined data between 2003 and 2016 to compare ambulance utilization and time to hospital presentation across sex and age. Results: Young adults account for 3.9% of 463,310 stroke/transient ischemic attack/hemorrhage admissions. They have a higher proportion of hemorrhage (37% vs. 15%) and fewer ischemic events (50% vs. 68%) compared with older patients. Younger patients are less likely to arrive by ambulance (62% vs. 66%, p < 0.001), with younger women least likely to use ambulance services (61%) and older women most likely (68%). Median stroke onset to hospital arrival times were 7 h for older patients and younger men, but 9 h in younger women. There has been no improvement among young women in ambulance utilization since 2003, whereas ambulance use increased in all other groups. Conclusions: Younger adults, especially younger women, are less likely to use ambulance services, take longer to get to hospital, and have not improved in utilization of emergency services for stroke over 13 years. Targeted public health messaging is required to ensure younger adults seek emergency stroke care.
MiRNA-12129 Suppresses Cell Proliferation and Block Cell Cycle Progression by Targeting SIRT1 in GASTRIC Cancer

Gastric cancer is the most commonly occurring cancer with a rapidly increasing incidence rate worldwide. The underlying molecular mechanisms of gastric cancer require further investigation. MicroRNAs exhibit tissue sensitivity as tumor biomarkers that play a role by promoting tumor growth as oncogenes or tumor suppressor genes. We evaluated the effects of microRNA-12129 on gastric cancer and identified the underlying mechanisms of microRNA-12129. Quantitative real-time polymerase chain reaction was conducted to determine the expression levels of microRNA-12129 and sirtuin 1 in vivo and in vitro, and Western blot analysis was performed to detect sirtuin 1 at the protein level in gastric cancer cell lines. Cell proliferation and cell cycle progression were detected by Cell Counting Kit-8 assay and flow cytometry analysis, respectively. The potential targets of microRNA-12129 were predicted by bioinformatics analysis. The targets of microRNA-12129 were confirmed by luciferase reporter assay and rescue assay. We found that microRNA-12129 was downregulated in gastric cancer tissues and gastric cancer cell lines and was significantly associated with the prognosis of patients with gastric cancer. In addition, microRNA-12129 overexpression suppressed tumor cell proliferation and blocked cell cycle progression. Bioinformatics analysis and luciferase reporter assay suggested that sirtuin 1 was a target of microRNA-12129, and sirtuin 1 expression was negatively related to microRNA-12129. Restoration of sirtuin 1 partly reduced the inhibition of cell proliferation and cell cycle progression induced by microRNA-144. Our results collectively suggested that microRNA-12129 suppressed cell proliferation and cell cycle progression in gastric cancer by targeting sirtuin 1. These findings indicated that manipulation of microRNA-12129 expression could help develop a novel therapeutic strategy for gastric cancer.
Sleep Quality as a Mediator of the Relationship Between Cyber Victimization and Depression.

PURPOSE Cyber victimization is a national mental health concern, especially among adolescents who are digital natives. The current study examined sleep quality as a mediator of the association between cyber victimization and depressive symptoms among adolescents. DESIGN AND METHOD A prospective study design was utilized with a community sample of adolescents (N = 801; 57% female; mean age = 14.45, SD = .85) from the eastern United States. Participants completed (a) the Pittsburgh Sleep Quality Index; (b) the Cyber Victimization Scale; and (c) the Center for Epidemiologic Studies Depression Scale Revised via online surveys at baseline and 6-month follow-up. The inter-relationship between variables was analyzed by Hayes' mediation approach. FINDINGS Cyber victimization was not directly associated with having depressive symptoms 6 months later when controlling for adolescents' poor sleep quality, sex, and age (direct effect [c'] = .012, t(676) = 1.12, p < . 05, confidence interval [CI] -. 008, . 036). The mediation analysis indicated a significant indirect effect of poor sleep quality on the relationship between cyber victimization and depressive symptoms (ab = .005, bootstrapped standard error [SE] = .003, bootstrapped CI .001, .011; a is the effect of cyber victimization on poor sleep quality; b is the effect of poor sleep quality on depressive symptoms). Specifically, adolescents' cyber victimization led to poor sleep quality (a = .039, SE = .041, p < .05), which also led to increased depressive symptoms (b = .116, SE = .019, p < .001), after controlling for depressive symptoms at baseline, sex, and age. The indirect effect of cyber victimization on depressive symptoms was estimated through poor sleep quality (a*b = .039(.116) = . 0045). CONCLUSIONS The findings suggest that poor sleep quality may be a mechanism through which cyber bullying is related prospectively to depressive symptoms. Interventions for cyber-victimized adolescents should include assessment of sleep quality and incorporate sleep hygiene education. CLINICAL RELEVANCE Adolescents should be screened for cyber victimization and sleep quality. Moreover, promotion of sleep hygiene among cyber-victimized adolescents may help to reduce depression.
Effect of rare coding variants in the CFI gene on Factor I expression levels

Abstract Factor I (FI) is one of the main inhibitors of complement activity, and numerous rare coding variants have been reported in patients with age-related macular degeneration, atypical hemolytic uremic syndrome and C3 glomerulopathy. Since many of these variants are of unknown clinical significance, this study aimed to determine the effect of rare coding variants in the complement factor I (CFI) gene on FI expression. We measured FI levels in plasma samples of carriers of rare coding variants and in vitro in the supernatants of epithelial cells expressing recombinant FI. FI levels were measured in 177 plasma samples of 155 individuals, carrying 24 different rare coding variants in CFI. In carriers of the variants p.Gly119Arg, p.Leu131Arg, p.Gly188Ala and c.772G>A (r.685_773del), significantly reduced FI plasma levels were detected. Furthermore, recombinant FI expression levels were determined for 126 rare coding variants. Of these variants 68 (54%) resulted in significantly reduced FI expression in supernatant compared to wildtype (WT). The recombinant protein expression levels correlated significantly with the FI level in plasma of carriers of CFI variants. In this study, we performed the most comprehensive FI expression level analysis of rare coding variants in CFI to date. More than half of CFI variants lead to reduced FI expression, which might impair complement regulation in vivo. Our study will aid the interpretation of rare coding CFI variants identified in clinical practice, which is in particular important in light of patient inclusion in ongoing clinical trials for CFI gene supplementation in AMD.
Adventitious root initiation in hypocotyl and epicotyl cuttings of eastern white pine (Pinus strobus) seedlings

The present paper reports results of experiments to develop a system for studying adventitious root initiation in cuttings derived from seedlings. Hypocotyl cuttings of 2-week-old eastern white pine (Pinus strobus L.) seedlings were treated for 5 min with 0, 100, 200, 300, 400, 500 or 600 mg 1-' (0, 0.54, 1.07, 1.61, 2.15, 2.69 or 3.22 mM) 1-naphthaleneacetic acid (NAA) to determine the effect on root initiation. The number of root primordia per cutting was correlated with NAA concentration and the square of NAA concentration. Thus, the number increased from less than one per cutting in the 0 NAA treatment to approximately 40 per cutting at 300 mg 1 -1 NAA, above which no substantial further increase was observed. The larger number of root primordia formed in response to increasing concentrations of NAA was due to the formation of primordia over a larger proportion of the hypocotyls. Histological analysis of the timing of root primordium formation in hypocotyl cuttings revealed three discernible stages. Progression through these stages was relatively synchronous among NAA-treated hypocotyl cuttings and within a given cutting, but variation was observed in the portion of different cuttings undergoing root formation. Control-treated hypocotyl cuttings formed root primordia at lower frequencies and more slowly than NAA-treated cuttings, with fewer primordia per cutting. Epicotyl cuttings from 11-week-old seedlings also formed adventitious roots, but more slowly than hypocotyl cuttings. NAA treatment of epicotyl cuttings caused more rapid root initiation and also affected the origin of adventitious roots in comparison with nontreated cuttings. NAA-treated epicotyl cuttings formed roots in a manner analogous to that of the hypocotyl cuttings, directly from preformed vascular tissue, while control-treated epicotyl cuttings first formed a wound or callus tissue and subsequently differentiated root primordia within that tissue. This system of inducing adventitious roots in pine stem cuttings lends itself to studying the molecular and biochemical steps that occur during root initiation and development.
Twitter usage about autism spectrum disorder

Stakeholders within autism spectrum disorder communities use Twitter for specific purposes. The goal of this study was to characterize patterns and themes of tweet content and sentiment and intercommunications between users sending and retweeting content to their respective user networks. The study used cross-sectional analysis of data generated from Twitter. Twitter content, sentiment, users, and community networks were examined from a sample of tweets with the highest Twitter reach and the lowest Twitter reach. Results indicate that Twitter content from both samples was primarily related to empowerment and support. Differences between the number of tweets originating from an individual in the lowest reach sample (i.e. 41%) as compared to the individuals in the highest reach sample (i.e. 18%) were noted. The number of users belonging to an advocacy subcommunity was substantially larger than a clinical and research subcommunity. Results provide insight into the presuppositions of individuals with autism spectrum disorder, their families and significant others, and other stakeholders.
Use of multiple lines of evidence to understand reactive mercury concentrations and chemistry in Hawai'i, Nevada, Maryland, and Utah, USA.

To advance our understanding of the mercury (Hg) biogeochemical cycle, concentrations and chemistry of gaseous oxidized Hg (GOM), particulate bound Hg (PBM) and reactive Hg (RM = GOM + PBM) need to be known. The UNR-RMAS 2.0 provides a solution that will advance knowledge. From 11/2017 to 02/2019, the RMAS 2.0 was deployed in Hawai'i, Nevada, Maryland, and Utah to test system performance and develop an understanding of RM at locations impacted by different atmospheric oxidants. Mauna Loa Observatory, Hawai'i, impacted by the free troposphere and the marine boundary layer, had primarily -Br/Cl RM compounds. The Nevada location, directly adjacent to a major interstate highway and experiences inputs from the free troposphere, exhibited -Br/Cl, -N, -S, and organic compounds. In Maryland, compounds observed were -N, -S, and organic Hg. This site is downwind of coal fired power plants and located in a forested area. The location in Utah is in a basin impacted by oil and natural gas extraction, multiday wintertime inversion episodes, and inputs from the free troposphere. Compounds were -Br/Cl or -O, -N, and -Br/Cl. The chemical forms of RM identified were consistent with the air source areas, predominant ion chemistry, criteria air pollutants, and meteorology.
Changes in the microflora of Valdeteja raw goat's milk cheese throughout manufacturing and ripening

Changes in the microbiological flora of Valdeteja, a ripened home-made raw goat's milk cheese produced in northwest Spain, were studied during the manufacture and ripening processes using the spiral plating method. High counts of all microbial groups were observed in milk (7.66, 7.57, 6.13, 6.91, 2.47, 5.18, 3.27 and 3.85 log10 cfu/g for aerobic mesophilic bacteria, lactococci, lactobacilli, leuconostocs, enterococci, Enterobacteriaceae,Micrococcaceae and moulds and yeasts, respectively). Counts increased between 0.76 and 1.96 log units from milk to curd. Lactic acid bacteria (lactococci, lactobacilli and leuconostocs) were the dominant microorganisms throughout ripening. Lactococci dominated over the lactobacilli up to day 27 of ripening. Leuconostocs were stable from day 2 of ripening. The final levels of typical enterococci were 3.62 log10 cfu/g. Aerobic mesophilic bacteria, Enterobacteriaceae andMicrococcaceae reached their maximum in curd and decreased significantly throughout ripening. The decrease ofEnterobacteriaceae was very marked, although they did not completely disappear at the end of the ripening process (2.74 log10 cfu/g). Moulds and yeasts counts increased significantly during ripening, giving final levels of 6.09 log10 cfu/g. A strong correlation (P<0.001) was found between pH and lactobacilli (R=−0.87), leuconostocs (R=−0.82) and moulds and yeasts (R=−0.74) and between aw and various microbial groups (e.g. EnterobacteriaceaeR=0.83).