Miracle-dz/UniFAR-FTUs huggingface dataset:
The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera

Rat basophilic leukaemia (RBL) cells have been used for decades as a model of high-affinity Immunoglobulin E (IgE) receptor (FcεRI) signalling. Here, we describe the generation and use of huNPY-mRFP, a new humanised fluorescent IgE reporter cell line. Fusion of Neuropeptide Y (NPY) with monomeric red fluorescent protein (mRFP) results in targeting of fluorescence to the granules and its fast release into the supernatant upon IgE-dependent stimulation. Following overnight sensitisation with serum, optimal release of fluorescence upon dose-dependent stimulation with allergen-containing extracts could be measured after 45 min, without cell lysis or addition of any reagents. Five substitutions (D194A, K212A, K216A, K226A, and K230A) were introduced into the FcεRIα cDNA used for transfection, which resulted in the removal of known endoplasmic reticulum retention signals and high surface expression of human FcεRIα* in huNPY-mRFP cells (where * denotes the penta-substituted variant), comparable to the ~500,000 FcεRIα molecules per cell in the RS-ATL8 humanised luciferase reporter, which is a human FcεRIα/FcεRIγ double transfectant. The huNPY-mRFP reporter was used to demonstrate engagement of specific IgE in sera of Echinococcus granulosus-infected individuals by E. granulosus elongation factor EgEF-1β and, to a lesser extent, by EgEF-1δ, which had been previously described as IgE-immunoreactive EgEF-1β/δ.
Radiological Features for Outcomes of MOGAD in Children: A Cohort in Southwest China

Background Studies suggested that myelin oligodendrocyte glycoprotein antibody-associated disorders (MOGAD) are an isolated group of diseases that are different from multiple sclerosis (MS) and neuromyelitis optica spectrum disorders (NMOSD). The proportion of individuals with MOGAD is higher among children. However, limited data are available on autoimmune antibodies and neuroimaging features in children with MOGAD. Methods This study retrospectively reviewed 42 children with MOGAD. The clinical, neuroradiological, and cerebrospinal fluid data were compared according to courses and radiological results. Results Of the 42 patients, 28 suffered a monophasic course and 14 had a relapsing course. During the follow-up magnetic resonance imaging (MRI), 21 patients had a well-resolved brain condition and another 21 patients showed slight improvement with marked residuals. Most patients with relapse had cortical lesions and a leukodystrophy-like MRI pattern (all p < 0.05). Children with poor radiological outcomes have confluent and hazy lesions that involve both cortexes, white matter lesion of >2 cm, and a leukodystrophy-like pattern, as well as cerebral lesions with T1 hypointensity or enhancement and spinal lesions (all p < 0.05). The multivariable logistic regression analysis used the aforementioned differential features and showed cerebral enhancement and a leukodystrophy-like pattern as the most effective variations associated with poor radiological outcomes of MOGAD with an area under the curve of 0.875. Conclusion MOGAD in children have some radiological features suggestive of clinical courses and radiological outcomes. A good understanding of these differential features can help to give early warnings of disease recurrence or poor radiological improvement and develop subsequent therapeutic strategies.
Adaptive Federated Deep Reinforcement Learning for Proactive Content Caching in Edge Computing

With the aggravation of data explosion and backhaul loads on 5 G edge network, it is difficult for traditional centralized cloud to meet the low latency requirements for content access. The federated learning (<underline>F</underline>L)-based <underline>p</underline>roactive content <underline>c</underline>aching (FPC) can alleviate the matter by placing content in local cache to achieve fast and repetitive data access while protecting the users’ privacy. However, due to the non-independent and identically distributed (Non-IID) data across the clients and limited edge resources, it is unrealistic for FL to aggregate all participated devices in parallel for model update and adopt the fixed iteration frequency in local training process. To address this issue, we propose a distributed resources-efficient FPC policy to improve the content caching efficiency and reduce the resources consumption. Through theoretical analysis, we first formulate the FPC problem into a stacked autoencoders (SAE) model loss minimization problem while satisfying resources constraint. We then propose an adaptive FPC (AFPC) algorithm combined deep reinforcement learning (DRL) consisting of two mechanisms of client selection and local iterations number decision. Next, we show that when training data are Non-IID, aggregating the model parameters of all participated devices may be not an optimal strategy to improve the FL-based content caching efficiency, and it is more meaningful to adopt adaptive local iteration frequency when resources are limited. Finally, experimental results in three real datasets demonstrate that AFPC can effectively improve cache efficiency up to 38.4<inline-formula><tex-math notation="LaTeX">$\%$</tex-math><alternatives><mml:math><mml:mo>%</mml:mo></mml:math><inline-graphic xlink:href="guo-ieq1-3201983.gif"/></alternatives></inline-formula> and 6.84<inline-formula><tex-math notation="LaTeX">$\%$</tex-math><alternatives><mml:math><mml:mo>%</mml:mo></mml:math><inline-graphic xlink:href="guo-ieq2-3201983.gif"/></alternatives></inline-formula>, and save resources up to 47.4<inline-formula><tex-math notation="LaTeX">$\%$</tex-math><alternatives><mml:math><mml:mo>%</mml:mo></mml:math><inline-graphic xlink:href="guo-ieq3-3201983.gif"/></alternatives></inline-formula> and 35.6<inline-formula><tex-math notation="LaTeX">$\%$</tex-math><alternatives><mml:math><mml:mo>%</mml:mo></mml:math><inline-graphic xlink:href="guo-ieq4-3201983.gif"/></alternatives></inline-formula>, respectively, compared with traditional multi-armed bandit (MAB)-based and FL-based algorithms.
Application of New-Generation Growth Regulators and Topdressing Nitrogen Fertilizers Increases Improver Winter Wheat Yield and Grain Quality in South Russia

The need for effective nutrient management is crucial for optimizing wheat production and increasing the plant’s ability to tolerate different environmental stresses. In this study, we assessed the influence of new-generation PGRs and foliar topdressing nitrogen fertilizer and their interactive effects on wheat yield and quality parameters of grain and flour. A three-year field experiment was conducted on the dark chestnut soil of the Rostov region. We estimated the effect of foliar topdressing liquid nitrogen fertilizer on consumption rates of N32 (N32), N64 (N32 + N32), and N96 (N32 + N32 + N32), as well as new-generation plant growth regulators (PGRs), namely, Zirkon, Silk, and Albit, on the productivity and grain quality of winter wheat Tarasovskaya 70. The results of the experiments indicate that the highest average grain yield over three years was 5.34 t/ha−1 with the application of N96 (N32 + N32 + N32) and Albit PGR. In 2020, due to favorable weather conditions, the greatest grain yield was attained at 6.27 t/ha−1 for N96 (N32 + N32 + N32) and Albit PGR. The highest grain quality with the greatest gluten content >28% was obtained when N96 (N32 + N32 + N32) and PGRs were applied. According to the results, using N96 (N32 + N32 + N32) and PGRs made it possible to obtain “valuable” grain in terms of quality, which is suitable for the production of premium flour. The beneficial interactive effects of N application rates and PGRs on winter wheat yield and quality parameters are worthy of further investigation.
Does Corruption Discourage Entrepreneurship?*

Abstract Although entrepreneurship plays a key role in economic development, its precise effect remains largely unknown. This is because it is challenging to objectively measure entrepreneurship and identify its determinants. In this paper, we analyze the effect of a particular feature of the institutional landscape, namely, corruption, on entrepreneurship. It is expected that corruption discourages entrepreneurship because it undermines fair competition. We use two proxies for entrepreneurship that are widely used in the literature: (1) nascent entrepreneurship collected from Global Entrepreneurship Monitor, and (2) entry rate defined as the number of new firms divided by the total number of previous year's registered businesses, collected from World Bank Group Entrepreneurship Survey. We find that better control of corruption promotes entrepreneurship. Our evidence is stronger when we use entry rate as a proxy of entrepreneurship. Our findings are preserved when we add other determinants of entrepreneurship drawn from the literature. When we use legal origins as instruments for corruption, our results remain essentially the same. The size of population, a proxy for market size, is positively associated with entrepreneurship while corporate taxes are negatively associated.
Study of output spectrum and optimization of the composition of toluene-based liquid scintillator

Scintillators in general and organic liquid scintillator specifically are widely used as a medium for the detection of charged particles for numerous applications in science, medicine, engineering, and other areas. The composition of the scintillator affects not only its direct performance characteristics, but also the overall cost. Optimization of this composition provides the ability to design particle detectors with an optimized light yield and emission spectra of the detection medium while optimizing the expenses at the same time. This article describes work on toluene-based liquid scintillator component optimization, where PPO is used as a fluor and POPOP as a shifter. The light yield vs. concentration and the changes in the output spectra will be presented. The empirical fit of the output spectrum using the measured contributions of the components is discussed. Further plans include the light attenuation measurements for different compositions.
Formulation of Magneto-Responsive Hydrogels from Dually Cross-Linked Polysaccharides: Synthesis, Tuning and Evaluation of Rheological Properties

Smart hydrogels based on natural polymers present an opportunity to fabricate responsive scaffolds that provide an immediate and reversible reaction to a given stimulus. Modulation of mechanical characteristics is especially interesting in myocyte cultivation, and can be achieved by magnetically controlled stiffening. Here, hyaluronan hydrogels with carbonyl iron particles as a magnetic filler are prepared in a low-toxicity process. Desired mechanical behaviour is achieved using a combination of two cross-linking routes—dynamic Schiff base linkages and ionic cross-linking. We found that gelation time is greatly affected by polymer chain conformation. This factor can surpass the influence of the number of reactive sites, shortening gelation from 5 h to 20 min. Ionic cross-linking efficiency increased with the number of carboxyl groups and led to the storage modulus reaching 103 Pa compared to 101 Pa–102 Pa for gels cross-linked with only Schiff bases. Furthermore, the ability of magnetic particles to induce significant stiffening of the hydrogel through the magnetorheological effect is confirmed, as a 103-times higher storage modulus is achieved in an external magnetic field of 842 kA·m−1. Finally, cytotoxicity testing confirms the ability to produce hydrogels that provide over 75% relative cell viability. Therefore, dual cross-linked hyaluronan-based magneto-responsive hydrogels present a potential material for on-demand mechanically tunable scaffolds usable in myocyte cultivation.
On the Relationship Between Stickiness in DMRT Theory and Physical Parameters of Snowpack: Theoretical Formulation and Experimental Validation With SNOWPACK Snow Model and X-Band SAR Data

This study aims at relating the stickiness parameter (<inline-formula> <tex-math notation="LaTeX">$\tau$ </tex-math></inline-formula>) of the dense media radiative transfer theory in quasi-crystalline approximation of Mie scattering of densely packed sticky spheres (DMRT-QMS), to the physical parameters of the layered snowpack. A relationship has been derived to express <inline-formula> <tex-math notation="LaTeX">$\tau $ </tex-math></inline-formula>, which modulates the attractive contact force between ice spheres, as a function of ice volume fraction (<inline-formula> <tex-math notation="LaTeX">$\phi $ </tex-math></inline-formula>) and coordination number (<inline-formula> <tex-math notation="LaTeX">$n_{c}$ </tex-math></inline-formula>). Since <inline-formula> <tex-math notation="LaTeX">$\tau $ </tex-math></inline-formula> is not a measurable parameter, this is a step forward with respect to what is commonly made in the literature, where <inline-formula> <tex-math notation="LaTeX">$\tau $ </tex-math></inline-formula> is assumed as an arbitrary parameter, generally ranging between 0.1 and 0.3, to fit simulated backscattering data with those measured. As a first validation, DMRT-QMS was integrated with the SNOWPACK model to simulate backscattering at X-band (9.6 GHz) driven by nivo-meteorological data acquired on a test area located in Monti Alti di Ornella, Italy. The simulations were compared with Synthetic Aperture Radar COSMO-SkyMed (CSK) satellite observations. The results show a significant agreement (<inline-formula> <tex-math notation="LaTeX">$R^{2} =0.68$ </tex-math></inline-formula>), although for a limited dataset of eight points in a unique winter season.
Single Injection of Cross-Linked Hyaluronate in Knee Osteoarthritis: A 52-Week Double-Blind Randomized Controlled Trial

Background: to compare the 52-week effectiveness and safety between HYAJOINT Plus (HJP) and Durolane in knee osteoarthritis (OA) treatment. Methods: consecutive patients received a single injection of 3 mL HJP or Durolane. The primary outcome was a visual analog scale (VAS) pain measurement at 26 weeks post-injection. Secondary outcomes included other clinical, satisfaction, and safety assessments for 52 weeks. Results: 142 patients were equally randomized. At week 26, the HJP group had less VAS pain than the Durolane group (18.1 ± 9.5 versus 24.4 ± 14.0, p = 0.001). Both groups showed improvement in their VAS pain and stiffness scores, and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain and total scores for 52 weeks after injection (p < 0.001). However, the HJP group showed lower VAS pain and stiffness scores, reduced WOMAC pain and stiffness scores, a shorter Timed “Up & Go” (TUG) time, and a higher satisfaction score than the Durolane group for 39 weeks (p < 0.05). Only mild and self-limited adverse events occurred (40.8%). Conclusion: While a single injection of either HJP or Durolane is safe and effective for at least 52 weeks, HJP provided superior improvement in terms of VAS pain and stiffness scores, WOMAC pain and stiffness scores, and satisfaction score within 39 weeks of treatment.
Arms Control and Nonproliferation: A Catalog of Treaties and Agreements [March 7, 2012]

Arms control and nonproliferation efforts are two of the tools that have occasionally been used to implement U.S. national security strategy. Although some believe these tools do little to restrain the behavior of U.S. adversaries, while doing too much to restrain U.S. military forces and operations, many other analysts see them as an effective means to promote transparency, ease military planning, limit forces, and protect against uncertainty and surprise. Arms control and nonproliferation efforts have produced formal treaties and agreements, informal arrangements, and cooperative threat reduction and monitoring mechanisms. The pace of implementation for many of these agreements slowed during the Clinton Administration, and the Bush Administration usually preferred unilateral or ad hoc measures to formal treaties and agreements to address U.S. security concerns. But the Obama Administration resumed bilateral negotiations with Russia and pledged its support for a number of multilateral arms control and nonproliferation efforts. The United States and Soviet Union began to sign agreements limiting their strategic offensive nuclear weapons in the early 1970s. Progress in negotiating and implementing these agreements was often slow, and subject to the tenor of the broader U.S.-Soviet relationship. As the Cold War drew to a close in the late 1980s, the pace of negotiations quickened, with the two sides signing treaties limiting intermediate range and long-range weapons. But progress again slowed in the 1990s, as U.S. missile defense plans and a range of other policy conflicts intervened in the U.S.-Russian relationship. At the same time, however, the two sides began to cooperate on securing and eliminating Soviet-era nuclear, chemical, and biological weapons. Through these cooperative efforts, the United States now allocates more than $1 billion each year to threat reduction programs in the former Soviet Union. The United States is also a prominent actor in an international regime that attempts to limit the spread of nuclear weapons. This regime, although suffering from some setbacks in recent years in Iran and North Korea, includes formal treaties, export control coordination and enforcement, U.N. resolutions, and organizational controls. The Nuclear Nonproliferation Treaty (NPT) serves as the cornerstone of this regime, with all but four nations participating in it. The International Atomic Energy Agency not only monitors nuclear programs to make sure they remain peaceful, but also helps nations develop and advance those programs. Other measures, such as sanctions, interdiction efforts, and informal cooperative endeavors, also seek to slow or stop the spread of nuclear …
Comparative Evaluation of 3 Commercial Mouthwash Formulations on Clinical Parameters of Chronic Gingivitis

Background Chlorhexidine (CHX) is not prescribed as a mouthwash for long-term use; therefore, probiotic/herbal mouthwashes are being investigated. This study compared the effect of 3 commercial mouthwashes on plaque index (PI), gingival index (GI), and bleeding index (BI) in patients with chronic gingivitis. Material/Methods Forty-five patients (all with moderate plaque) were randomly allocated into 3 groups (Gp): Gp 1 (CHX), Gp 2 (Manuka), and Gp 3 (Pro-Dental). Three periodontal clinical parameters – PI, GI, and BI – were recorded at baseline and on days 7, 14, and 28. An oral hygiene maintenance program was followed by a double-blinded intervention (coded bottle containing mouthwash). Both inter-group and intra-group comparisons were made using analysis of variance (ANOVA) with multiple t tests. All probable values were considered to have various levels of significance at P<0.05 or below. Results All indices for all groups showed higher values (mean) at baseline, which were lower on days 7, 14, and 28. No differences in any clinical parameter at any point of time existed between Gp 1 and Gp 2. There were, however, significant differences (P<0.05) between Gp 1/Gp 3 and Gp 2/Gp 3 for all clinical parameters at all observed time periods (days 7, 14, 28). Intra-group comparison for all groups demonstrated highly significant differences between baseline values and other time points. Conclusions For managing chronic gingivitis, Manuka mouthwash is as effective as a CHX mouthwash, as there were no differences observed in any clinical parameters at any point points.
Learning from Each Other: Comparing the Experiences of First-Generation Migrant, International, and Domestic Students at Irish Universities

Despite the potential for mutual learning, improvement of service provision, and the advancement of policy, research on first-generation migrant students and research on international students have remained stubbornly separate. This paper makes the case that education research would benefit from leveraging data on international students and that internationalization research and practice should more often include first-generation migrant students. To explore the benefits of this proposition, this article explores differences in the experiences of Irish-born domestic students, first-generation migrant students, and international students at Irish higher education institutions, using Eurostudent VI data collected in 2016. These comparisons show that first-generation migrant students report lower levels of preparation for the domestic labor market than both Irish-born domestic students and international students. International students are faring well on other indicators too, as they report higher levels of academic satisfaction and feeling more prepared for the international labor market than both first-generation migrant students and domestic students. Results also suggest that the academic satisfaction of the three groups of students is shaped by both common as well as divergent factors, indicating that interventions aimed to support each group may require additional tailoring. However, limitations of both quantitative and qualitative approaches to documenting the experiences of international students need to be considered as findings from this study and similar studies are used. By comparing the experiences of the three groups of students, this paper highlights the experience of first-generation migrant students, often overlooked in international higher education studies.
Gene Set Enrichment Analysis Detected Immune Cell-Related Pathways Associated with Primary Sclerosing Cholangitis

Aim To explore various immune cell-related causal pathways for primary sclerosing cholangitis (PSC). Methods Immune cell-related pathway association study was conducted via integrative analysis of PSC GWAS summary and five immune cell-related eQTL datasets. The GWAS summary data of PSC was driven from 4,796 PSC cases and 19,955 healthy controls. The eQTL datasets of CD4+ T cells, CD8+ T cells, B cells, natural killer cells (NK), monocytes, and peripheral blood cells (PB) were collected from recently eQTL study. The PSC GWAS summary dataset was first aligned with eQTL datasets of six blood cells to obtain the GWAS summary data at overlapped eQTL loci, separately. For each type of cell, the obtained PSC GWAS summary dataset of eQTLs was subjected to pathway enrichment analysis. 853 biological pathways from Kyoto Encyclopedia of Genes and Genomes, BioCarta, and Reactome pathway databases were analyzed. Results We identified 36 pathways for B cells, 33 pathways for CD4+ T cells, 28 pathways for CD8+ T cells, 33 pathways for monocytes (MN), 35 pathways for NK cells, and 33 for PB cells (all empirical P values <5.0 × 10−5). Comparing the pathway analysis results detected 25 pathways shared by five immune cells, such as KEGG_CELL_ADHESION_MOLECULES_CAMS (P value <5.0 × 10−5) and REACTOME_MHC_CLASS_II_ANTIGEN_ PRESENTATION (P value <5.0 × 10−5). Several cell-specific pathways were also identified, including BIOCARTA_INFLAM_PATHWAY (P value <5 × 10−5) for B cell. Conclusion Our study holds potential to identify novel candidate causal pathways and provides clues for revealing the complex genetic mechanism of PSC.