Miracle-dz/UniFAR-FTUs huggingface dataset:
Combined Liquid Biopsy Methylation Analysis of CADM1 and MAL in Cervical Cancer Patients

Simple Summary In cervical neoplasia, tissue methylation of CADM1 and MAL has been identified as a promising triage tool for HPV-positive women. Here, we performed an exploratory study on the potential of the methylated genes as combined markers for the analysis of cervical smears and peripheral blood samples in cervical cancer patients. The combination of both markers in liquid biopsy resulted in a sensitivity of 83.3% and specificity of 95.5% for discrimination of cervical cancer from healthy controls. The robustness of the assay was proven by a respectably high sensitivity in stage I-II patients. Abstract Cervical cancer is the fourth most common cancer in women, which is associated in >95% with a high-risk human papillomavirus (HPV) infection. Methylation of specific genes has been closely associated with the progress of cervical high-grade dysplastic lesions to invasive carcinomas. Therefore, DNA methylation has been proposed as a triage for women infected with high-risk HPV. Methylation analyses of cervical cancer tissue have shown that cell adhesion molecule 1 (CADM1) and myelin and lymphocyte protein (MAL) methylation are present in over 90% of all cervical high-grade neoplasias and invasive cervical cancers. Here, we established a liquid biopsy-based assay to detect MAL and CADM1 methylation in cell free (cf)DNA of cervical cancer. Methylation of the target gene was validated on bisulfite converted smear-DNA from cervical dysplasia patients and afterward applied to cfDNA using quantitative real-time PCR. In 52 smears, a combined analysis of CADM1 and/or MAL (CADM1/MAL) showed methylation in 86.5% of the cases. In cfDNA samples of 24 cervical cancer patients, CADM1/MAL methylation was detected in 83.3% of the cases. CADM1/MAL methylation was detected already in 81.8% of stage I-II patients showing the high sensitivity of this liquid biopsy assay. In combination with a specificity of 95.5% towards healthy donors (HD) and an area under the curve (AUC) of 0.872 in the receiver operating characteristic (ROC) analysis, CADM1/MAL cfDNA methylation detection might represent a novel and promising liquid biopsy marker in cervical cancer.
Association of Biomarkers with Individual and Multiple Body Sites of Pain: The Johnston County Osteoarthritis Project

Introduction Biochemical biomarkers may provide insight into musculoskeletal pain reported at individual or multiple body sites. The purpose of this study was to determine if biomarkers or pressure-pain threshold (PPT) were associated with individual or multiple sites of pain. Methods This cross-sectional analysis included 689 community-based participants. Self-reported symptoms (ie, pain, aching, or stiffness) were ascertained about the neck, upper back/thoracic, low back, shoulders, elbows, wrist, hands, hips, knees, ankles, and feet. Measured analytes included CXCL-6, RANTES, HA, IL-6, BDNF, OPG and NPY. A standard dolorimeter measured PPT. Logistic regression was used determine the association between biomarkers and PPT with individual and summed sites of pain. Results Increased IL-6 and HA were associated with knee pain (OR=1.30, 95% CI 1.03, 1.64) and (OR=1.32, 95% CI 1.01, 1.73) respectively; HA was also associated with elbow/wrist/hand pain (OR=1.60, 95% CI 1.22, 2.09). Those with increased NPY levels were less likely to have shoulder pain (OR=0.56, 95% CI 0.33, 0.93). Biomarkers HA (OR=1.50, 95% CI 1.07, 2.10), OPG (OR=1.74, 95% CI 1.00, 3.03), CXCL-6 (OR=1.75, 95% CI 1.02, 3.01) and decreased PPT (OR=3.97, 95% CI 2.22, 7.12) were associated with multiple compared to no sites of pain. Biomarker HA (OR=1.57, 95% CI 1.06, 2.32) and decreased PPT (OR=3.53, 95% CI 1.81, 6.88) were associated with multiple compared to a single site of pain. Conclusion Biomarkers of inflammation (HA, OPG, IL-6 and CXCL-6), pain (NPY) and PPT may help to understand the etiology of single and multiple pain sites.
Associations between Psychological Variables, Knowledge, Attitudes, Risk Perceptions and Health Behaviours towards COVID-19 among Adolescents

There is currently little scientific evidence available that allows us to understand patterns of knowledge, risk perception, attitudes, and behaviours among adolescents in relation to COVID-19. This study aims to analyse the relationship between knowledge about COVID-19, risk perception, and psychological variables and the adherence to preventive measures among the adolescent population. It is a descriptive cross-sectional study, which included adolescents between the ages of 12 and 18 (n = 354). The questionnaire was sent to several secondary schools chosen by convenience sampling and following a non-probabilistic snowball sampling. Descriptive, univariate, and multivariate analyses were carried out in order to determine whether knowledge about COVID-19, risk perception, tolerance of frustration, planning and decision-making, family functionality, self-efficacy, self-esteem, and social skills are related to preventive measures. The adoption among adolescents of behaviours which protect them against COVID-19 depends on knowledge about the disease, the perception of the risk it poses to them, as well as their tolerance of frustration and planning and decision-making abilities. The relationship between the individual variables among adolescents with the adoption of behaviours which protect them against COVID-19 has been confirmed. The development of intervention and communication strategies that take the psychosocial situation of adolescents into account will help to increase the adoption of protective health behaviours in the context of a pandemic.
Exemplar based Inpainting using Autoregressive Parameter Estimation

In this paper, we propose an iterative exemplar based inpainting technique. A set of exemplars is automatically searched considering a window around every pixel in the region to be inpainted. The novelty of our technique lies in the use of parameters of an autoregressive (AR) model that are estimated using the non-negatively constrained least squares (NNLS) method. Unlike simply copying into the target or damaged pixels (i.e. pixels to be inpainted), values from the best matching exemplar as is done by many of the exemplar based methods, we use the AR parameters along with the best matching exemplar to modify the values of the damaged pixels. The AR parameters for a set of candidate exemplars suggest the contribution of values of neighbouring pixels towards the respective center pixel of every 3× 3 region in that set. Determining a pixel value with the knowledge of spatial relationship of pixels and information from an exemplar avoids discontinuity that arises due to direct copying of pixels from the exemplar. Results are illustrated with the help of true images captured from the world heritage site Hampi, Karnataka.
Green Information System (GIS) Model in the Conference Sector: Exploring Attendees’ Adoption Behaviors for Conference Apps

Introduction Academic conferences are carbon-intensive. Conference travel by air contributes to huge amount of global greenhouse gas emissions, which can be reduced by adopting green information systems (green IS or GIS). This study aims to investigate attendees’ behaviors for adopting green IS (such as conference apps) from an ecological perspective. Methods For this research, a survey method was used. Survey instrument had 29 items adapted from existing scales. Data was collected using Mturk’s panel of respondents on online Qualtrics. Exploratory and confirmatory factor analysis were conducted on 403 responses for data analysis and validation of the results using SPSS AMOS ver. 24. Findings This study provides empirical evidence to add ecological beliefs as a key predictor of green IS adoption models. Findings demonstrate that attitudes and ecological beliefs play an essential role in conference app adoption, in comparison with performance expectations, social impacts, and facilitation conditions, as presented in the traditional technology acceptance literature. Findings assist conference owners develop essential communication strategies for green attendees.
Optical Microscope Rehabilitation Nursing Study of Anterior Cruciate Ligament Injury through Lateral Knee Incision Based on Medical Internet of Things

With the high development of sports, football has attracted more and more attention from the public. However, the hot competition has made football players undergo high-intensity training, and the risk of injury caused by training is also increasing. Lateral incision knee ACL injury is one of the most common types of injuries in football players, which has a serious impact on the athlete's physiology and daily training. The comprehensive, multi-level, convenient and fast medical system brought by the medical Internet of Things has become the growing demand of the medical industry. Based on the medical Internet of Things, this paper studies the rehabilitation nursing of football players' ACL injury by lateral cutting and running combined with optical microscope. In this paper, 36 male and female football players were selected for group experiments, and the landing and peak torque indexes of the experimental group and the control group of male and female athletes were analyzed under the observation of an optical microscope. Group (P <0.05), coxa valgus (P <0.01), internal rotation and knee valgus angles were greater than those in the EM group. And the peak hip flexion angle and knee valgus moment in NF group were lower than those in EF group (P <0.05). After 12 weeks of rehabilitation training, there was a significant difference in the Q/H joint contraction index between the REF group and the CON group (P <0.05), and there was no statistical difference in the other groups. After 12 weeks of rehabilitation training, there was a significant difference in the Q/H joint contraction index between the REF group and the CON group (P <0.05), and there was no statistical difference in the other groups. That is to say, after the systematic rehabilitation training proposed in this paper, the joint contraction performance of the hamstrings/quadriceps in the REF group has been significantly improved. This shows that the rehabilitation nursing under the optical microscope based on the medical Internet of Things has a good effect on the rehabilitation of the football player's side-cut running knee joint ACL injury.
Basic Concepts in Quantum Information

In the last 25 years a new understanding has evolved of the role of information in quantum mechanics. At the same time there has been tremendous progress in atomic/optical physics and condensed matter physics, and particularly at the interface between these two formerly distinct fields, in developing experimental systems whose quantum states are long-lived and which can be engineered to perform quantum information processing tasks. These lecture notes will present a brief introduction to the basic theoretical concepts behind this `second quantum revolution. ' These notes will also provide an introduction to `circuit QED. ' In addition to being a novel test bed for non-linear quantum optics of microwave electrical circuits which use superconducting qubits as artificial atoms, circuit QED offers an architecture for constructing quantum information processors.
Characteristics and Presentation of Women with Ectopic Pregnancy

Objective: To determine characteristics and presentation of women with ectopic pregnancy (EP). Study Design: A cross-sectional study. Place and Duration of the Study: The Department of Obstetrics & Gynaecology, Sialkot Medical College, Sialkot Pakistan from January 2021 to December 2021. Material and Methods: A total of 39 women aged between 18 to 45 years and diagnosed with EP were included. Age, parity status, clinical presentation, diagnostic modality and sides affected were noted. A special proforma was designed to record all study data. For statistical analysis, SPSS version 26.0 was employed. Numeric data was shown as mean and standard deviation while categorical variables were represented as frequencies/percentages. Results: In a total of 39 women with ectopic pregnancy, mean age was 28.6±6.4 years while 23 (59.0%) women were aged between 18 to 30 years. There were 9 (23.1%) women who were nulliparous. Residential status of 22 (56.4%) women was rural in 22 (56.4%) patients. Condition of tube revealed ruptured EP among 37 (94.9%) women. Most common clinical presentation was abdominal/pelvic pain in 37 (94.9%) women while amenorrhea and vaginal bleeding were observed in 32 (82.1%) and 30 (76.9%) women respectively. Conclusion: Most commonly observed clinical presentation among women with ectopic pregnancy were abdominal/pelvic pain, amenorrhea and vaginal bleeding while almost half of the patients presented with shock. Keywords: Amenorrhea, abdominal pain, ectopic pregnancy, shock, vaginal bleeding.
Role of Diffusion-Weighted Magnetic Resonance Imaging in Prediction of Pathological Complete Response to Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer and Its Molecular Subtypes

Purpose The aim of this study was to evaluate the role of apparent diffusion coefficient (ADC) and hence diffusion-weighted imaging in prediction of pathological complete response (pCR) to neoadjuvant chemotherapy (NACT) in locally advanced breast cancer (LABC) and its molecular subtypes. Methods In this tertiary hospital-based prospective study, 30 patients aged 30 to 65 years, having clinically/cytologically diagnosed LABC, were included. Magnetic resonance imaging (MRI) was done to obtain prechemotherapy ADC (ADC pre ), postchemotherapy ADC (ADC post ), change in ADC (ΔADC), and ΔADC% for each tumor and its subtype. Postsurgical pCR was used as the reference standard for determining tumor response. All four ADC parameters were compared between pCR and non-pCR groups. Results Of the 30 patients, 19 (63.3%) patients showed pCR, while 11 (36.7%) patients did not. The pCR group showed significantly lower mean ADC pre ( p < 0.001) and higher mean ADC post ( p < 0.05), ΔADC, and ΔADC% ( p = 0.000) than non-pCR group. The best cutoff values to differentiate responders from nonresponders with receiver operating characteristic curve analysis of ADC pre , ADC post , and ΔADC% were 0.98 × 10 −3 mm 2 /s (68.4% sensitivity, 63.6% specificity), 1.31×10 −3 mm 2 /s (68.4% sensitivity, 63.6% specificity), and 25% (84.2% sensitivity, 90.9% specificity), respectively. Human epidermal growth factor receptor 2 (HER2)-enriched subtype showed significant difference in mean ADC pre ( p = 0.045), while triple-negative subtype showed significant differences in mean ADC post ( p = 0.032) and mean ΔADC ( p = 0.019) between the two groups. Conclusion ADC pre , ADC post , and ΔADC can predict pCR to NACT in LABC. Among molecular subtypes, ADC pre was predictive only in HER2-enriched subtype, while ADC post and ΔADC were predictive only in triple-negative subtype.
Dietary Factors and the Risk of Gastric Cancer in Hanzhong Area of China

Background: Dietary factors play an important role in gastric cancer risk. They have not been investigated extensively in Hanzhong area, China. Methods: We conducted a population-based case-control study of gastric cancer in Hanzhong area, China in 2018–2020. A total of 121 patients with historically confirmed gastric adenocarcinomas were interviewed. Controls were an age-stratified random sample of residents of Hanzhong area. The dietary questionnaire was a 70-item semiquantitative food frequency adapted for the Hanzhong diet. Odds ratios were calculated for quartiles of consumption of food groups and were adjusted for age, gender, calories, chili pepper intake, cigarette smoking, socioeconomic status, added salt, and history of peptic ulcer disease. Results: There was approximately a threefold increased risk of gastric cancer for frequent consumption (highest quartile) of both fresh meat (odds ratio (OR) = 3.0) and processed meat (OR = 3.3). Odds ratios were also significantly elevated for frequent consumption of dairy products (OR = 3.1) and fish (OR = 3.1). The authors observed a decreasing gradient of risk with increasing frequency of vegetable consumption due to a significant inverse trend for the yellow and orange vegetables. Conclusion: High intake of citrus fruits showed a slight inverse association. These findings are consistent with many studies around the world that indicate important roles for salt, processed meats, and vegetable consumption in gastric cancer risk.
Therapeutic Potential of Glucagon-like Peptide-1 Agonists in Polycystic Ovary Syndrome: From Current Clinical Evidence to Future Perspectives

Despite the continuous effort to understand the pathophysiology and determine potential therapeutic targets, PCOS treatment largely depends on lifestyle intervention and symptomatic management of individual signs and symptoms. International guidelines recognize the importance of weight reduction as a cornerstone for the achievement of better metabolic, reproductive, and cardiovascular outcomes in PCOS women who are overweight or obese. With its profound weight loss potential in patients with or without diabetes, the administration of GLP-1 receptor agonists has been investigated in overweight/obese women with PCOS in several single-center randomized control trials with considerable variation in the dosing regimen, follow-up duration, and outcome measurements over recent years. Most trials reported superior weight loss effects of GLP-1 receptor agonists compared to lifestyle changes or metformin, with additional metabolic, reproductive, and cardiovascular benefits in this population. However, their use is currently not widely accepted by the clinical community that treats this population. The major concern is how to balance the reproductive and metabolic treatment strategies since the use of GLP-1 receptor agonists requires effective contraception while on therapy and a washout period before pregnancy. Both approaches are not mutually exclusive, yet the best choice requires a careful assessment of the clinical context. Knowing a patient’s individual circumstances, precise clinical sub-phenotyping, and regular monitoring are crucial components for the safe and effective use of these new tools. In the present narrative review, we explore the current clinical evidence and provide the future perspectives and challenges for their implementation in PCOS management.
The Role of Green HRM on Environmental Performance of Hotels: Mediating Effect of Green Self-Efficacy & Employee Green Behaviors

ABSTRACT Environmental issues have emerged to be a significant challenge for the organizations in twenty-first century and they are striving hard to reduce their environmental footprint. Considering the emerging environmental issues, the study investigates the role of green human resource management (GHRM) toward the environmental performance of hotels. Additionally, the study also focuses on the mediating effect of employees’ green self-efficacy and employee green behavior. The target population consisted of green and non-green hotel employees. Data was collected using a survey questionnaire from 600 employees (300 from green hotels and 300 from non-green hotels) and analyzed through SMART PLS. The findings show that green human resource practices lead to a higher hotel environmental performance. Additionally, motivated employees are highly efficacious then they get engaged in performing the green behavior, leading to enhanced hotel environmental performance. Based on the comparative analysis, this study proposes that HR of the hospitality sector should extensively focus on GHRM practices. The study offers the valuable insights regarding how the HR can boost the self-efficacy of the employees regarding their contribution toward environmental performance improvement.
Modelisation mathematique et resolution numerique de problemes de fluides compressibles a plusieurs constituants

Le but de ce travail est de resoudre des problemes de fluides euleriens compressibles a plusieurs constituants. Les constituants peuvent etre melanges ou non. Dans le cas ou ils ne le sont pas, ils sont separes par des interfaces. Ce travail comporte une partie de modelisation mathematique des melanges et une partie consacree a la resolution numerique des modeles proposes. Dans la partie de modelisation, nous proposons une classe de modeles derivee de principes thermodynamiques. Les modeles sont donnes sous forme d'equations aux derivees partielles conservatives ou non. Nous nous attachons a decrire leurs proprietes mathematiques : symetrisabilite, hyperbolicite, existence de solutions-ondes progressives dans la partie de resolution numerique, nous commencons par etudier un probleme modele : l'advection lineaire. Pour la resolution numerique de cette equation, nous proposons un algorithme de type volumes finis qui presente la particularite de n'etre pas dissipatif : nous obtenons une estimation d'erreur uniforme en temps. Nous proposons ensuite une extension de cet algorithme aux equations scalaires non lineaires convexes, pour lesquelles nous effectuons une etude detaillee de la convergence entropique. Nous etendons ensuite ce schema a la resolution des equations d'euler. Ceci permet un calcul optimal des discontinuites de contact (localisees sur une unique maille). Ce schema est ensuite utilise pour le transport des fractions massiques des constituants dans un algorithme de type lagrange-projection pour la resolution des modeles de fluides a plusieurs constituants. Cela permet un calcul optimal des interfaces (situees sur une unique maille) ainsi que des bords des zones de melange. Nous demontrons des estimations entropiques pour chaque constituant. Nous montrons des resultats numeriques obtenus en dimensions 1 et 2.
Unusual glassy states in LaMn0.5Fe0.5O3: Evidence of two distinct dynamical freezing processes

An interesting scenario of unusual glassy states is observed from the dc and ac susceptibility measurements in LaMn0.5Fe0. 5O3. The dc measurements at 5mT exhibit two distinct peaks at ∼20(Tg) and ∼255K(Tp). The large field-cooled effect and frequency-dependent results indicate a glassy state between Tg and Tp, while the relaxation dynamics does not show cooperative relaxation without aging, suggesting the existence of noninteracting clusters of frozen spin. On the other hand, the glassy state with clear evidence of a cooperative relaxation process is noticed below Tg, which has been characterized from the field- and frequency-dependent results associated with the evidence of aging. The nature of the cooperative glassy state does not meet the glassy features of classical spin-glass compounds.