Miracle-dz/UniFAR-FTUs huggingface dataset:
Amyloid β attenuates mZnR/GPR39-dependent Ca 2+ , ERK1/2 and Clusterin signaling in neurons

A hallmark of Alzheimer’s disease is accumulation of amyloid beta (Aβ) deposits, which are associated with neuronal dysfunction, spine loss and impaired Ca 2+ homeostasis. Amyloid beta (Aβ) binds to and is aggregated by Zn 2+ , a metal released from synaptic glutamatergic vesicles during neuronal activity. Synaptically released Zn 2+ activates a metabotropic Gq-coupled Zn 2+ -sensing receptor, mZnR/GPR39, and induces Ca 2+ -signaling in postsynaptic neurons. We asked if Aβ, as a Zn 2+ binding protein, regulates neuronal Zn 2+ signaling mediated by mZnR/GPR39 using SHSY-5Y cells and cortical neurons from GPR39 wildtype and knockout mice. Following acute or chronic treatment with Aβ neuronal Zn 2+ -dependent Ca 2+ release via mZnR/GPR39 is significantly reduced. This impairment is overcome when excess Zn 2+ is applied, suggesting that impaired Ca 2+ signaling results from Aβ binding of Zn 2+ . The Zn 2+ -dependent mZnR/GPR39 activation triggers phosphorylation of extracellular regulated kinase (ERK1/2) and upregulates expression of the chaperone protein clusterin (Clu). Importantly, neuronal Zn 2+ -dependent ERK1/2 phosphorylation and upregulation of Clu are attenuated by silencing mZnR/GPR39 as well as by Aβ treatment. In contrast, Zn 2+ -dependent AKT phosphorylation is not mediated by mZnR/GPR39 and is not attenuated by Aβ treatment. Thus, Zn 2+ signaling via mZnR/GPR39 is distinctively disrupted by a critical pathological component of Alzheimer’s disease. of Initial rates of the responses determined and presented in the bar graphs, averaged over 3-5 coverslips. Representative traces from one coverslip (averaged over about 10 cells) are shown for each paradigm and rates of initial Ca 2+ rise were calculated from all traces (coverslips) obtained. The initial rate of the ligand-dependent metabotropic Ca 2+ response represents the response directly elicited by the receptor and does not depend on other pathways that are involved in Ca 2+ removal from the cytoplasm (Verkhratsky 2004). Initial rates of fluorescence change from all traces were averaged to give the rate per experimental paradigm. ∆R/s is the average of initial rate over at least 10 coverslips per experimental condition, and is presented in the bar graphs. a novel pathway for maintaining neuronal function and enhancing neuronal survival in Alzheimer’s disease.
A Study of the Relation Between Nursing Students’ Global Competences and Their Multiple Intelligences for the Fourth Industrial Revolution

본 연구는 4차 산업혁명을 위한 간호대학생의 글로벌 인재역량과 다중지능간의 관계를 알아보기 위한 서술적 상관관계 연구이다. 연구 대상은 전남과 전북에 소재한 4년제 간호학과 4개교에 재학중인 대학생 총 768명을 대상으로 편의 추출하였고, 총 768부중 733부가 분석되었다. 분석결과, 성별에 따른 글로벌 인재 역량.
Flattening the Curve, Mindfully -- Can Meditation Apps Help to Mitigate the Psychological Impacts of Social Distancing Measures during COVID-19?

The present study is an inquiry into the question whether meditation apps can mitigate some of the adverse psychological impacts that individuals commonly experience during pandemics. In a randomized controlled trial, subjects either practice meditation via Headspace or cognitive training via Lumosity for one week. Thereafter, the two groups are compared on loneliness and anxiety levels. The evidence of the results in this study suggest that a week of mindfulness training through a meditation app is not sufficient to have a significant impact on a person’s psyche .
Consiglio Nazionale delle Ricerche Understanding Vehicle-to-Vehicle IEEE 802.11p Beaconing Performance in Real-World Highway Scenarios

Periodic exchange of situational information (beacons) is at the basis of most active safety applications in vehicular environments. Despite its fundamental role in raising the level of “situational awareness” onboard vehicles, very little is known to date on beaconing performance in a real vehicular environment. This paper analyzes the results of two measurement campaigns that have been designed with the purpose of disclosing beaconing performance in a variety of vehicular links, for what concerns vehicle conﬁguration (tall/short), line-of-sight conditions (LOS/NLOS), as well as single-hop or two-hop propagation of the information reported in the beacons. For the ﬁrst time, beaconing performance is characterized in terms of not only the packet (beacon) delivery rate (PDR), but also in terms of the packet (beacon) inter-reception (PIR) time. The latter metric has been suggested in the literature as more accurately measuring the level of “situation awareness” onboard vehicles than the traditional PDR metric. This paper also presents a simulation-based analysis aimed at estimating the beneﬁt of multi-hop propagation of situational information beyond the second hop of communication. The analysis of the data collected in the measurement campaigns as well as the simulation-based analysis disclose a number of interesting insights which might prove useful in the design of active safety applications. Finally, another major contribution of this paper is promoting the Gilbert-Elliot model, previously proposed to model bit-error bursts in packet switched networks, as a very accurate model of beacon reception behavior observed in real-world scenarios.
C/EBP Factors in Human Neutrophils Cytokine Production through CREB and The p38-MSK1 Signaling Cascade Influences

Neutrophils inﬂuence innate and adaptative immunity by generating numerous cytokines and chemokines whose regulation largely depends on transcriptional activators such as NF- k B and C/EBP factors. In this study, we describe the critical involvement of CREB transcription factors (CREB1 and activating transcription factor-1) in this functional response as well as relevant upstream signaling components. Neutrophil stimulation with LPS or TNF led to the phosphorylation, DNA binding activity, and chemokine promoter association of CREB1 and activating transcription factor-1. These responses occurred downstream of the p38-MSK1 signaling axis, as did the phosphorylation and promoter association of another bZIP factor, C/EBP b . Conversely, inhibition of RSK1 failed to alter the phosphorylation of either CREB1 or C/EBP b in neutrophils. From a more functional standpoint, the inhibition of p38 MAPK or MSK1 interfered with cytokine generation in neutrophils. Likewise, overexpression of a dominant-negative CREB1 mutant (K-CREB) or of a point mutant (S133A) resulted in a decreased ability of human neutrophil-like PLB-985 cells to generate inﬂammatory cytokines (CXCL8, CCL3, CCL4, and TNF- a ). Collectively, our data show the involvement of CREB1 in neutrophil cytokine production, the key role of its S133 residue, important upstream signaling events, and the parallel activation of another bZIP factor. These are all potential molecular targets that could be exploited in the context of several chronic inﬂammatory diseases that prominently feature neutrophils and their products. The study, we report on the expression, subcellular localization, phosphorylation, DNA-binding activity, and cytokine promoter occupancy of CREB transcription factors in human neutrophils stimulated by physiological agonists. We also demonstrate the involvement of CREB1 in cytokine production, the key role of its Ser 133 residue for this response, and the upstream signaling events controlling CREB1 activation and that of bZIP
Translating the impact of knowledge management into knowledge‐based innovation: The neglected and mediating role of knowledge‐worker satisfaction

The purpose of knowledge management is innovation. However, this study proposes that the existence of knowledge management in the knowledge‐worker's work environment can nurture the overall satisfaction of knowledge worker. As the ultimate purpose of knowledge management is innovation performance, therefore, the satisfaction of knowledge worker as an outcome of knowledge management should be greater innovation performance. Consequently, the purpose of this study is to test the mediating role of satisfaction of knowledge worker between knowledge management and innovation. The study collected the data from 306 knowledge workers (engineers and managers) of software houses from Pakistan. The SmartPLS 3 Version 2.7 software that uses the PLS‐SEM (Partial Least Square‐Structural Equation Modelling) technique was used. The results indicate that satisfaction of knowledge worker mediates between two knowledge management processes (knowledge creation and knowledge sharing) and innovation significantly. However, it does not mediate between knowledge utilization and innovation significantly.
The probability of a nucleotide sequence formed by random mutations

I analyze the stochastic formation of a nucleotide sequence based on Dawkins’ weasel algorithm, according to which each nucleotide can mutate with a certain probability and natural selection favors the individuals closest to the ideal genetic information. I provide an analytical solution to this algorithm, from which it is possible to obtain a probability distribution and the average number of generations necessary to build a functional nucleotide sequence. From these results, I estimate a lower limit of the time when the split between the genera Pan and Homo should at least have occurred and compare it with empirical data, according to which it happened some million years ago. I also show that this lower limit is also valid for more sophisticated algorithms than Dawkins’.
University of Birmingham Measurement of iodine monoxide at a semi polluted coastal location

Point source measurements of IO by laser induced ﬂuorescence spectroscopy were made at a semi-polluted coastal location during the Reactive Halogens in the Marine Boundary Layer (RHaMBLe) campaign in September 2006. The site, on the NW French coast in Roscoff, was characterised by extensive intertidal macroalgae beds which were exposed at low tide. The closest known iodine active macroalgae beds were at least 300 m from the measurement point. From 20 days of measurements, IO was observed above the instrument limit of detection on 14 days, of which a clear diurnal proﬁle was observed on 11 days. The maximum IO mixing ratio was 30.0 pptv (10 s integration period) during the day, amongst the highest concentrations ever observed in the atmosphere, and 1–2 pptv during the night. IO concentrations were strongly dependent on tidal height, the intensity of solar irradiation and meteorological conditions. An intercomparison of IO measurements made using point source and spatially averaged DOAS in-Correspondence to: D. E. Heard (d.e.heard@leeds.ac.uk) struments conﬁrms the presence of hot-spots of IO caused by an inhomogeneous distribution of macroalgae. The co-incident, point source measurement of IO and ultra ﬁne particles (2.5 nm ≥ d ≥ 10 nm) displayed a strong correlation, providing evidence that IO is involved in the production pathway of ultra ﬁne particles at coastal locations. Finally, a modelling study shows that high IO concentrations which are likely to be produced in a macrolagae rich environment can signiﬁcantly perturb the concentrations of OH and HO 2 radicals. The effect of IO on HO x is reduced as NO x concentrations increase.
The possibilities of automation of the manual line for dismantling waste electrical and electronic equipment

The authors introduced automatic processing line for electrical and electronical equipment wastes as an alternative for semi-automatic WEEE lines. The efficiency of semi-automatic line was characterized for small WEEE with LCA and AHP algorithms. The problem nodes showed by algorithms was optimized and replaced by automatic devices. Optimized automatic WEEE processing line generated `groups of sources with min. 98 wt% purity. Comparison of the results obtained for the semi-automatic line to the automated line, 83-times increase in productivity was observed. Streszczenie. W artykule przedstawiono automatyczną linię do przetwarzania odpadów elektrycznych i elektronicznych (WEEE) jako alternatywę dla linii półautomatycznych. Wydajność linii półautomatycznej charakteryzowała się małą wydajnością przetwarzania WEEE. Do oceny możliwości poprawy jej wydajności wykorzystano algorytmy LCA i AHP. Węzły problemów wskazane przez algorytmy zostały zoptymalizowane i zastąpione przez urządzenia automatyczne. Zoptymalizowana automatyczna linia do przetwarzania WEEE generuje materiały o minimalnej czystości 98% wag. Porównanie wyników uzyskanych dla linii półautomatycznej i linii zautomatyzowanej wykazało 83-krotny wzrost wydajności. (Możliwości automatyzacji ręcznej linii do demontażu zużytego sprzętu elektronicznego).
Finding the Missing Baryons Using CMB as a Backlight

We present a new method for detecting the missing baryons by generating a template for the kinematic Sunyaev-Zel'dovich effect. The template is computed from the product of a reconstructed velocity field with a galaxy field; we find that the combination of a galaxy redshift survey such as SDSS and a CMB survey such as ACT and PLANCK can detect the kSZ, and thus the ionized gas, at significant signal-to-noise. Unlike other techniques that look for hot gas or metals, this approach directly detects the electrons in the IGM through their signature on the CMB. The estimated signal-to-noise for detecting the galaxy-momentum kSZ cross-correlation is 4, 9, and 12 for ACT (with survey area of 2000 $\mathrm{deg}^2$) with SDSS-DR4, SDSS3 and ADEPT respectively. The estimated signal-to-noise for PLANCK with SDSS-DR4, SDSS3 and ADEPT is 11, 23, and 32. Our method provides a new mean for determining properties of the ionized gas in the Universe. We provide galaxy momentum templates constructed from Sloan Digital Sky Survey online at our website at this http URL The predicted correlation coefficients are provided along with the momentum maps. One can download the momentum templates and cross-correlate directly with CMB maps from ACT and PLANCK to detect the missing baryons.
New Experimental Model for Basic Research in Stem Cell Field

One of biotechnological application of stem cells is in tissue engineering filed. Combining stem cells with biomaterial scaffolds provides a promising strategy for engineering tissues and cellular delivery to develop biological substitutes that can restore tissue functions. Even though there are some obstacles to the generation of functional tissues and this aspect limited their widespread clinical use, mesenchymal stem cells are the best lines available to a possible application in regenerative medicine. Thanks to their large capacity for self-renewal while maintaining their multipotency MSC can differentiate into a variety of cell types, including osteoblasts, chondrocytes and adipocytes.
Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation

is controlled by Vav2 and β PIX. These guanine nucleotide exchange factors regulate the spatiotemporal dynamics of Rac3 activity, impacting GIT1 localization. Moreover, the GTPase-activating function of GIT1 toward the vesicular trafficking regulator Arf6 GTPase is required for matrix degradation. Importantly, Rac3 regulates the ability of tumor cells to metastasize in vivo. The Rac3-dependent mechanisms we show in this study are critical for balancing proteolytic activity and adhesive activity to achieve a maximally invasive phenotype.
Using mesh hernioplasty to patient with inguinal Hernia

To clinically evaluate the outcome of Inguinal Hernioplasty in terms of haematoma, wound infection and recurrence, with special reference to surgery done by trainee surgeons. A total of 50 patients with inguinal hernia were operated during the study period. The result of the present study concluded that using mesh for surgical operation of hernia is much better than the ordinal operation of hernia because this technique reduces the recurrence of the disease, while the classical operation is related to recurrence of the disease. Also Mesh can reduce the appearing of hematomas, Wound infections after the operation, while the classical operation is related to appearing of the hematomas and Wound infections after the operation. Further more there is no significant difference between the two methods regarding appearing of seromas after the operation however, its appear among those patients which did a classical operation more than those which using mesh for operation. So In our set-up Mesh Hernioplasty has proven to be effective with low complication and recurrence rates.
Employment Creation and Poverty Reduction in Nigeria: A Case of Farmers’ Cooperative Societies in Kano State, Nigeria.

This study examines the role of employment creation on poverty reduction among members of farmers’ cooperative societies in Kano State Nigeria. A cross-sectional survey research design was adopted. Primary data collection method was used in which self-administered questionnaire were distributed to a total sample of 217 respondents selected from the target population of 498 members of farmers’ multi-purpose cooperative societies. Pearson correlation and binary logistic regression model were used to determine the influence of employment creation on poverty reduction among the respondents. The mean per capita expenditure was used as a dependent variable, while employment creation as the independent variable. The findings indicated that female were more affected by poverty than their male counterparts. Gender was not a significant factor that determined poverty level in this research. Young and middle age were the majority of the respondents and these age groups were more affected by poverty. Age was found as a significant factor that determined poverty level in this research. Majority of the respondents had household size of 7 to above 12, and the respondents with large family size were more affected by poverty than those with small ones. The household size was found as a significant factor that determined poverty level in this research. Majority of the respondents had low educational qualifications, and the respondents with low educational qualifications were more affected by poverty than those with higher educational qualifications. The educational qualification was found as a significant factor that determined poverty level in this research. The results further showed that employment was found as statistically significant factors that contributed towards poverty reduction among members of farmers’ cooperative societies in this study, and the study recommended that age, family size, educational qualifications, and given adequate soft loans should be considered when designing employment creation programmes for poverty reduction. Farmers’ cooperative societies.
Genomic DNA preparation and transposon insertion site sequencing

uses the Type secretion system to escape predation by the soil-dwelling amoeba Abstract Mycobacterium bovis is the causative agent of bovine tuberculosis and the predominant 23 cause of zoonotic tuberculosis in people. Bovine tuberculosis occurs in farmed cattle but also in a variety of wild animals, which form a reservoir of infection. Although direct 25 transmission of tuberculosis occurs between mammals, the low frequency of contact 26 between different host species and abundant shedding of bacilli by infected animals, 27 suggests an infectious route via environmental contamination. Other intracellular 28 pathogens that transmit via the environment deploy strategies to survive or exploit 29 predation by environmental amoebae. To explore if M. bovis has this capability, we 30 investigated its interactions with the soil and dung-dwelling amoeba, Dictyostelium 31 discoideum . We demonstrated that M. bovis evades phagocytosis and destruction by 32 D. discoideum and actively transits through the amoeba using the ESX-1 Type VII Secretion 33 System as part of a program of mechanisms, many of which have been co-opted as virulence 34 factors in the mammalian host. This capacity of M. bovis to utilise an environmental stage 35 between mammalian hosts may enhance its transmissibility. Additionally, our data provide 36 molecular evidence to support an evolutionary role for amoebae as training grounds for the 37 pathogenic M. tuberculosis complex.
Ubiquitäre Systeme ( Seminar ) und Mobile Computing ( Proseminar ) WS 2015 / 16 Mobile und Verteilte Systeme Ubiquitous Computing Teil XIII Herausgeber :

Mobile sensing is an emerging area of interest for researchers all around the world. As smart devices like smartphones, smartwatches, etc. continuously increase their computational power and the amount of built-in sensors, the amount of new application cases for smart devices in everybody’s everyday life increases as well. The Doppler Effect as physical principal can leverage commodity hardware of these devices to improve interaction with digital interfaces or systems like smart-home environments. This paper provides an overview of a variety of applications that are build on top of the Doppler Effect and that are able make device interaction more comfortable and provide a rich human-computer interaction. It highlights the benefits that results from Doppler Effect based applications as well as possible challenges in the future.
Feedback Loop b Depends on an IFN-Murine B Cell Response to TLR7 Ligands

Type I IFNs play an important, yet poorly characterized, role in systemic lupus erythematosus. To better understand the interplay between type I IFNs and the activation of autoreactive B cells, we evaluated the effect of type I IFN receptor (IFNAR) deﬁciency in murine B cell responses to common TLR ligands. In comparison to wild-type B cells, TLR7-stimulated IFNAR (cid:2) / (cid:2) B cells proliferated signiﬁcantly less well and did not up-regulate costimulatory molecules. By contrast, IFNAR1 (cid:2) / (cid:2) B cells did not produce cytokines, but did proliferate and up-regulate activation markers in response to other TLR ligands. These defects were not due to a difference in the distribution of B cell populations or a failure to produce a soluble factor other than a type I IFN. Instead, the compromised response pattern reﬂected the disruption of an IFN- (cid:1) feedback loop and constitutively low expression of TLR7 in the IFNAR1 (cid:2) / (cid:2) B cells. These results highlight subtle differences in the IFN dependence of TLR7 responses compared with other TLR-mediated B cell responses. The Journal of Immunology, 2009, 183: 1569–1576. ng/ml CL097 (InvivoGen). AM14 B cells, speciﬁc for IgG2a, were stimulated with the RNA-speciﬁc mAb BWR4, provided by Dr. D. Eilat (Hadassah University Hospital, Jerusalem, Israel), at a ﬁnal concen- tration of 10 (cid:4) g/ml. The effect of type I IFN was determined by preincu-bating the cells with 300 U/ml IFN- (cid:1) or IFN- (cid:3) (PBL), unless another concentration is speciﬁcally noted, fo r 1 h at 37 o C before adding the var-ious ligands. B18R was obtained from eBioscience. B cell proliferation was as previously described (24). Brieﬂy, B cells were stimulated in 96-well plates at a ﬁnal concentration of 2 (cid:1) 10 6 cells/ml for 24 h, then pulsed for 6 h with [ 3 H]thymidine (Amersham Biosciences). Incorporation of [ 3 H]thymidine was quantiﬁed via a liquid scintillation beta counter (PerkinElmer). Forthecellmixture experiments, cells were cultured in 48-well plates at a ﬁnal concentration of 1.5 (cid:1) 10 6 cells/ml for 24 h. For some of the cultures, the allotype-disparate cells were mixed at a 1:1 ratio before stim- ulation; in other wells, the cells were combined at 1:1 volume ratio after stimulation but before ﬂow cytometric analysis. IgM allotype was deter- mined with mouse anti-IgM a -FITC and mouse anti-IgM b -PE (BD Bio-sciences). Analysis was conducted with FlowJo software (Tree Star). The following Abs were used to characterize splenic B cell populations: B220-Paciﬁc Blue, CD21-FITC, CD23-PE-Cy7, IgM-PE, and AA4.1-allophyco- cyanin