Miracle-dz/UniFAR-FTUs huggingface dataset:
Δ Np63 α Repression of the Notch1 Gene Supports the Proliferative Capacity of Normal Human Keratinocytes and Cervical Cancer Cells

The p53 family member p63 is a master regulator of epithelial development. One of its isoforms, Δ Np63 α , is predominantly expressed in the basal cells of stratified epithelia and plays a fundamental role in control of regenerative potential and epithelial integrity. In contrast to p53, p63 is rarely mutated in human cancers, but it is frequently overexpressed in squamous cell carcinomas (SCC). However, its functional relevance to tumorigenesis remains largely unclear. We previously identified the Notch1 gene as a novel transcriptional target of p53. Here, we show that Δ Np63 α functions as a transcriptional repressor of the Notch1 gene through the p53-responsive element. Knockdown of p63 caused upregulation of Notch1 expression and marked reduction in proliferation and clonogenicity of both normal human keratinocytes and cervical cancer cell lines overexpressing Δ Np63 α . Concomitant silencing of Notch1 significantly rescued this phenotype, indicating the growth defect induced by p63 deficiency to be, at least in part, attributable to Notch1 function. Conversely, overexpression of Δ Np63 α decreased basal levels of Notch1, increased proliferative potential of normal human keratinocytes, and inhibited both p53-dependent and p53-independent induction of Notch1 and differentiation markers upon genotoxic stress and serum exposure, respectively. These results suggest that Δ Np63 α maintains the self-renewing capacity of normal human keratinocytes and cervical cancer cells partly through transcriptional repression of the Notch1 gene and imply a novel pathogenetical significance of frequently observed overexpression of Δ Np63 α together with p53 inactivation in SCCs. Cancer Res; 70(10); 4034
Very small embryonic-like (VSEL) stem cells: recent knowledge

Very small embryonic-like stem cells (VSELs) are a unique and rare stem cell population, which have the same structural, genetic, biochemical, and functional characteristics as embryonic stem cells and they are able to differentiate to all three germ layers. In several emergency situations related to organ damage, VSELs can be activated and mobilized into peripheral blood, and in appropriate animal models they contribute to tissue organ/regeneration. Optimized methods for isolation and expansion of VSELs have aroused the scientific community's interest in use of this kind of cells for regenerative purposes. The properties of such pluripotent cells make them a potential candidate in regenerative medicine.
Rapid Prototyping of a Croatian Large Vocabulary Continuous Speech Recognition System

—The Croatian language, like many minority languages used by less than 0.1% of the world population, is in need of mature automatic speech recognition (ASR) systems for applications such as transcription of speech recordings, voice control, an aid to impaired people, etc. This paper describes a short-term research and development project aimed to produce an applicable Croatian large vocabulary continuous speech recognition system from scratch. The open-source CMU Sphinx toolkit was our platform choice. For the purpose of acoustic model training, we made a speech training set of several hundred utterances, containing words carefully chosen according to their phonetic properties. Language models were derived from the Croatian large-scale n-gram system, which ensures the system’s applicability. During the project, we succeeded in developing an ASR system able to recognize freely chosen utterances composed of 15,000 most frequently used Croatian words reasonably well.
Brief-Report COVID-19 Longitudinal associations of meaning in life and psychosocial adjustment to the COVID-19 outbreak in China

Objective. Literature suggests that ‘meaning in life’ may be a mental strength that enables individuals to function healthily and adaptively in the face of stress events. Therefore, this study aims to examine the longitudinal associations between meaning in life and psychosocial adjustment to the COVID-19 outbreak among Chinese people. Methods. A prospective design was adopted. 154 Chinese college students (Mean age = 20.41 (cid:1) 1.45 years) completed two waves of the assessment. Participants reported their meaning in life before the outbreak (Time 1) and their psychosocial adjustment 7 weeks later after the outbreak had occurred (Time 2). Results. Participants’ meaning in life at Time 1 was positively related to life satisfaction and negatively related to depression, anxiety, stress, and negative emotions at Time 2. Additionally, levels of meaning in life at Time 1 were positively associated to COVID-19-related behavioural engagement – prosocial behaviour and information addiction at Time 2. Individuals’ perceptions of the outbreak and status of self-quarantine did not moderate these relationships. Conclusion. Findings suggest that individuals’ prior level of meaning in life may help them maintain a healthy psychosocial adjustment during disease outbreak, though cautions regarding the possibility to render an addiction to information about the outbreak are warranted.
Numerical simulation of coupled cell motion and nutrient 1 transport in NASA’s rotating bioreactor 2

8 Rotating bioreactor, such as the NASA bioreactor, which was designed by the National Aeronautics 9 and Space Administration (NASA), USA, can be used to mimic micro-gravity conditions and simulate 10 the effects of microgravity on cells growth. The cell growth in the bioreactor depends on the nutrient 11 availability which in turn depends on the cell density and distribution within the bioreactor. In this 12 work, we use a numerical model of suspended particle motion to simulate the cell motion and 13 distribution in a specific variant of the NASA bioreactor, namely, the high aspect ratio vessel (HARV) 14 bioreactor. The nutrient distribution in the bioreactor is simulated based on a convection-diffusion- 15 reaction supplemented by laboratory experiments aimed at obtaining the required data. We present the 16 modelling framework in this paper and discuss the most salient simulated results. For example, the 17 simulation results show that the distributions of the cells in the bioreactor appear as concentric circles 18 and that the cells density is higher in the middle of the HARV bioreactor. These cell distributions 19 imply that they may accumulate in the middle of the bioreactor at sufficiently high cell density. The 20 results also demonstrate that the concentration of nutrient is fairly uniform in the bioreactor but 21 decreases slightly from the outer radius of the HARV bioreactor to the inner radius. This is possibly 22 caused by the higher consumption of glucose due to the higher cell density in the middle of the radius. 23 We expect that the modelling framework in this paper would help optimize the culture conditions for 24 the cells in HARV bioreactors.
Effect of different doses and methods of boron application on days to sprouting, plant height and yield per plant of potato ( Solanum tuberosum L.) cv. Kufri Surya in varied altitudes of Andhra Pradesh

A field experiment was conducted during rabi , 2016 and 2017 to study the effect of different doses and methods of boron application on growth, yield and quality of potato ( Solanum tuberosum L.) in varied altitudes of Andhra Pradesh. Plants were treated with different doses and three different methods of boron application viz., soil application @ 10, 15, 20 kg ha -1 , tuber dipping in 0.2, 0.4 and 0.6 % borax solutions, foliar application of borax concentrations 100, 200 and 300 ppm were freshly prepared and were sprayed at 30 DAP and control treatment. The results revealed that minimum number of days taken to sprouting, maximum plant height at 30 DAP, 60 DAP, before harvesting (80 DAP) and yield per plant was recorded with soil application of borax @ 20 kg ha -1 (T 4 ) during both the years of experimentation (2016-17 and 2017-18) and in pooled analysis.
Psychological Status and Health-Related Quality of Life and Associated Factors in Heart Failure Patients with Implantable Cardioverter Defibrillator

Introduction: Heart Failure (HF) patients receive Implantable Cardioverter Defibrillator (ICD) to improve survival rate from sudden cardiac death. Poor psychological condition and Health-Related Quality of Life (HRQoL) were reported in those patients. However, these two areas are under-reported in this specific population. Objective: To examine psychological status (depression and anxiety) and HRQoL and identify associated factors in HF patients with ICD. Methods: A cross-sectional design was conducted using a convenience sampling to recruit eligible patients at two acute general hospitals. Data were collected through the structured face-to-face interview. The self-reported Hospital Anxiety and Depression Scale (HADS) and Short Form-36 Health Survey (SF-36v2) were used for measuring anxiety and depression and HRQoL respectively. Results: About 11.8% and 22.1% of HF patients with ICD reported significant levels of anxiety and depression respectively. The results showed that physical component summary was poorer than mental component summary. Moreover, numerous factors were identified but only depression was negatively associated with both physical and mental health. Self-care dependence had positive association with both anxiety and depression. Conclusion: HF patients with ICD may perceive significant clinical anxiety and depression. Self-care dependence was significantly associated with anxiety and depression. Depression was the significant factor negatively associated with both physical and mental quality of life. However, anxiety was not associated with both physical and mental quality of life. The findings enrich the knowledge on psychological condition and HRQoL. Strategies targeting associated factors with psychological distress and HRQoL are crucial to improve care for this specific population. DM: Diabetes Mellitus; SE: Standard Error; CRT-D: Cardiac Resynchronization Therapy Defibrillator; HADS: Hospital Anxiety and Depression; HF: Heart Failure; HRQoL: Health-Related Quality of Life; ICD: Implantable Cardioverter Defibrillator; MCS: Mental Composite Summary; PCS: Physical Composite Summary; SCD: Sudden cardiac Death; SF-36v2: Short Form-36 Health Survey; SSQ6: Short Form Social Support Questionnaire; VF: Ventricular Fibrillation; VT: Ventricular Tachycardia
Measurement of the e(+)e(-) -> b(b)over-bar cross section between root s=10.54 and 11.20 GeV

We report e(+)e(-) -> b (b) over bar cross section measurements by the BABAR experiment performed during an energy scan in the range of 10.54 to 11.20 GeV at the SLAC PEP-II e(+)e(-) collider. A total relative error of about 5% is reached in more than 300 center-of-mass energy steps, separated by about 5 MeV. These measurements can be used to derive precise information on the parameters of the Y(10860) and Y (11020) resonances. In particular we show that their widths may be smaller than previously measured.
Cardiac volume overload rapidly induces oxidative stress-mediated myocyte apoptosis and hypertrophy

Oxidative stress stimulates both growth and apoptosis in cardiac myocytes in vitro. We investigated the role of oxidative stress in the initial phases of cardiac remodeling induced in an animal model by volume overload. As plausible candidates for a connection between oxidative stress and cardiomyocyte apoptosis or hypertrophy, we explored the behaviour of two MAPKs, specifically JNK and ERK. At 48 h of overload, the greatest increase in oxidative stress coincided with a peak of cardiomyocyte apoptosis. This was possibly induced through the mitochondrial metabolism, as evidenced by the release of cytochrome c and a significant increase in the active forms of caspase-9 and -3, but not caspase-8. Oxidative stress markers significantly decreased at 96 h of overload, combined with a marked attenuation of apoptosis and the appearance of hypertrophy. The highest levels of JNK and the lowest levels of ERK phosphorylation were observed at 48 h of overload. Conversely, a sharp increase in ERK phosphorylation was detected at 96 h of overload coinciding with the hypertrophic response. Together these results show that oxidative stress is an early and transient event in myocardial volume overload. They suggest that oxidative stress mediates amplitude dependent apoptotic and hypertrophic responses in cardiomyocytes through the selective activation of, respectively, JNK and ERK. D All rights reserved.
Clinical Healthcare Workers with SARS-CoV-2 infection in Mexico City

Background: We evaluated the risk of death of healthcare workers (HCW) with SARS-CoV-2 infection in Mexico City during the COVID-19 pandemic and described the associated factors in hospitalized HCW compared with non-HCW. Methods: We analyzed data from laboratory-confirmed SARS-CoV-2 cases registered from February 27-August 31, 2020 in Mexico City’s public database. Individuals were classified as non-HCW and HCW (subcategorized as physicians, nurses and other HCW). In hospitalized individuals, a multivariate logistic regression model was used to analyze potential factors associated with death and compare mortality risk among groups. Results: A total of 125,665 patients were included. Of these, 13.1% were HCW (28% physicians, 38% nurses and 34% other HCW). Compared with non-HCW, HCW were more frequently female, younger and free of comorbidities. Overall, 25,771 (20.5%) were treated as inpatients and 11,182 (8.9%) deaths were reported. Deaths in the total population and in hospitalized patients were significantly higher in non-HCW than in HCW (9.9% vs 1.9%, P <.001; and 39.6% vs 19.3%, P <.001, respectively). In hospitalized patients, using a multivariate model, the risk of death in HCW in general was lower (OR 0.53) compared to non-HCW, and by specific occupation, in physicians, nurses and other HCW risk was OR 0.60, 0.29, 0.61, respectively. Conclusions: HCW represent an important proportion of individuals with SARS-CoV-2 infection in Mexico City. While the mortality risk in HCW is lower compared with non-HCW, a high mortality rate in hospitalized patients was observed in this study. Among HCW, nurses had lower risk of death compared to physicians and other HCW. The public database includes clinical and demographic information on admission for all registered cases. For the present study, we included relevant variables such as age, sex, occupation, type of institution of care (facilities of the Ministry of Health, facilities of the Social Security system [IMSS] or [ISSSTE], other public institutions and private institutions), municipality of residency (options available were Mexico City, the state of Mexico and other states), date of symptom onset, date of first evaluation, comorbidities (diabetes, chronic obstructive pulmonary disease [COPD], asthma, immunosuppression, hypertension, cardiovascular disease, obesity, chronic kidney disease [CKD]), current smoking, treatment setting (outpatient or hospitalized), diagnosis of pneumonia, outcomes (ICU admission, invasive mechanical ventilation and death), and date of death. Outcomes in the database are updated upon report capacity from each medical institution to the SINAVE. We classified positive SARS-CoV-2 individuals from the original dataset, as HCW and non-HCW and compared both groups in terms of age, sex, treatment setting, number of comorbidities, current smoking, state of residency, and type of medical institution of care. HCW were further categorized in three groups: 1) doctors nurses, other workers deaths
Efficacy and Safety of Rituximab in Systemic Lupus Erythematosus and Sjögren Syndrome Patients With Refractory Thrombocytopenia A Retrospective Study of 21 Cases

Objective: Recent studies suggested a potential of rituximab (RTX) in treating autoimmune thrombocytopenia (AITP) secondary to autoimmune diseases. In this study, we retrospectively evaluated the efficacy and safety of RTX therapy in patients with refractory AITP secondary to systemic lupus erythematosus (SLE) and Sjögren syndrome (SS). Methods: Twenty-one SLE and/or SS patients with treatment-resistant AITP were treated once or repeatedly with RTX at the Rheumatology Clinic Renji Hospital, during the periodMarch 2012 to June 2014. Clinical and laboratory variables recorded at every follow-up visit were analyzed. Results: The median age of all patients was 37.05 ± 3.15 years (range, 13–67 years; 20 female and 1 male). The median AITP duration before RTX treatment was 5.46 years. Previous treatments of 21 patients included immunosuppressive agents such as corticosteroids (n = 19), cyclosporine (n = 9), mycophenolate mofetil (n = 2), methotrexate (n = 3), cyclophosphamide (n = 2), vincristine (n = 3), and hydroxychloroquine (n = 15), and 7 patients received concomitantly intravenous immunoglobulin therapy. Two patients had undergone splenectomy without improvement. Seventeen patients (80.95%) were treated repeatedly with RTX during the follow-up period. The overall response rate to RTX treatment (including complete response, 52.38%; partial response, 28.57%)was 80.95%. A significant increase (P < 0.05) of platelet counts was seen after 1 month (median, 32.24 10/mL vs 66.53 10/mL). Relapses occurred mostly during the first 9months, andmaintainingdurationof responsewas10.27months (range, 2–17months) on average after the first RTX infusion.Antiplatelet antibodies, especially IgGisotype,decreasedsignificantly (P<0.05) afterRTXtreatment. No adverse effects were observed among 15 patients (71.4%); however, 2 cases died of severe pneumonia, and another developed lymphoma. Conclusions: Rituximab is an additional potent therapeutic treatment option for SLE and SS patients with AITP refractory to conventional immunosuppressive treatments. For most patients, RTX was safe and well tolerated.
Systolic Blood Pressure, Sacubitril/Valsartan, and Cardiovascular Outcomes in Heart Failure with Preserved Ejection Fraction: PARAGON-HF

Background: Guidelines recommend targeting systolic blood pressure (SBP)<130 mmHg in heart failure with preserved ejection fraction (HFpEF) with limited data. Objectives : To assess whether BP lowering is associated with clinical benefit, and whether the treatment effects of sacubitril/valsartan on outcomes are related to BP lowering, particularly among women who derive greater benefit from sacubitril/valsartan. Methods: We analyzed 4,795 participants from the Prospective Comparison of ARNI with ARB Global Outcomes in HF with Preserved Ejection Fraction (PARAGON-HF) trial. We related baseline and time-updated, mean achieved SBP quartiles (<120, 120-129, 130-139, ≥140 mmHg) to the primary outcome (CV death and total HF hospitalization), its components, myocardial infarction or stroke, and a renal composite outcome. At the 16-week visit, we assessed the relationship between SBP change and Kansas City Cardiomyopathy Questionnaire overall summary score (KCCQ-OSS) and N-terminal pro-B-type natriuretic peptide (NT-proBNP). We analyzed whether the BP lowering effects of sacubitril/valsartan accounted for its treatment effects. Results: Average age was 73±8 years and 52% were women. After multivariable adjustment, baseline and mean achieved SBP 120-129mmHg demonstrated the lowest risk for all outcomes. Sacubitril/valsartan sacubitril/valsartan SBP (interaction p=0.50) and was similar when adjusting for time-updated SBP, irrespective of sex. Conclusions: Baseline and mean achieved SBP of 120-129 mmHg identified the lowest risk HFpEF patients. Baseline SBP did not modify the treatment effect of sacubitril/valsartan and the BP lowering effects of sacubitril/valsartan did not account for its effects on outcomes, irrespective of sex. ABSTRACT Guidelines recommend targeting SBP<130mmHg in HFpEF, but the clinical benefits of, and influence of sacubitril/valsartan on, BP lowering are unknown. In PARAGON-HF, baseline and mean achieved SBP 120-129mmHg was associated with the lowest risk for adverse outcomes. Sacubitril/valsartan reduced SBP by 5.2mmHg compared with valsartan. The associations between sacubitril/valsartan and outcomes were not modified by baseline SBP and was similar when adjusting for time-updated SBP. In sum, SBP 120-129 mmHg identified the lowest risk patients, supporting current guidelines. Baseline SBP did not modify the treatment effect of sacubitril/valsartan and the BP lowering effects of sacubitril/valsartan did not account for its effects on outcomes.
Effects of NP and Biofertilizers on Growth and Some Yield Attributes of Sunflower Helianthus Annus L

— A field experiment was carried out in the demonstration farm of Sudan University of Science and Technology, College of Agricultural Studies, Shambat, for two consecutive seasons 2014/15 and 2015/16 to determine the effects of synthetic N (urea) and P (superphosphate) fertilizers and/or microbial biofertilizers on plant height (cm), number of leaves per plant, shoot and root dry weights, head diameter and 1000 seed weight of the sunflower hybrid Shambat. Nitrogen and phosphorus were applied at 100 and 50 kg/feddan, respectively. Biofertilizers were applied as a mixture of Azospirillum Brasilense (a nitrogen fixer) and Bacillus megaterium var. phosphaticum (a phosphate solubilizer). A combination of synthetic and biofertilizers was applied in addition to the control. NP treatment gave the highest values of growth parameters in the first season followed by biofertilizers. In the second season biofertilzers then their combination produced the highest values. Head diameter and 1000 seed weight were enhanced by NP fertilizers. Significant differences in the first season were found only between NP and the combination of NP + AB in the number of leaves per plant and root dry weight, and in the second season the same treatments were significantly different only in shoot dry weight.
Indoor Source Localization Using 2D Multi-Sensor Based Spatial Spectrum Fusion Algorithm

— In a starving indoor environment where non-line of sight (NLOS) signals are strongly dominant, localization using traditional spatial spectrum estimation techniques easily fails due to low signal to noise ratio (SNR). Accordingly, in this paper, a novel 2-D multi-sensor Spatial Spectrum Fusion (2D-SSF) localization algorithm based on the multiple signal classification (MUSIC) method is proposed . The output data of each uniform rectangular array (URA) at each access point (AP) are first processed to get the noise subspace data. Then, after estimating the corresponding azimuth and elevation angles of each array using the MUSIC approach and finding the position of each point relative to each sensor in the search grid with the help of grid refinement algorithm, the parameters of interest of the target are estimated from a single spectrum that results from fusing all maximum noise subspaces where the position corresponding to the minimum error between the set of angles and every estimated point in the searching area is situated. Different simulation results of the proposed method in terms of RMSE as a function of SNR for various APs LOS/NLOS scenarios, the change in the number of antennas at each AP and the comparison with the MUSIC approach and the 1D localization based spatial spectrum fusion algorithm are carried out. The obtained results prove the significant performance of the proposed 2D-SSF localization algorithm with the strong presence of NLOS signals.