Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficiency of p16 and Ki-67 immunostaining for detecting premalignant cervical lesions in high risk population

Background: This study aimed to evaluate the efficiency of immunostaining with p16 and Ki-67 in cervical cytology specimens for the detection of cervical intraepithelial neoplasia (CIN) in a high risk population.Methods: This was a prospective review of 287 women who underwent pap smear, human papilloma virus (HPV) testing and colposcopy examination, respectively. There were cervical smear abnormalities in 108 women (37.6%) and 141 patients (49.1%) tested positive for HPV. Cervical biopsy revealed normal cervix in 28 patients (9.75%), cervicitis in 48 patients (16.72%), CIN1 in 178 patients (62.02%), CIN2 in 26 patients (9.05%) and CIN3 in 7 patients (2.43%). Results: Positive staining for p16 had a sensitivity of 78.2% and a specificity of 97.4% while positive staining for Ki67 had a sensitivity of 80.6% and a specificity of 57.9% for distinguishing CIN lesions in cervical cytology specimens (p=0.001 for both). Concurrent positive staining for p16 and Ki67 in cervical cytology specimens had a sensitivity of 80.6% and a specificity of 97.4% for CIN lesions (p=0.001). Positive staining for p16 had a sensitivity of 94% and a specificity of 90.6% whereas positive staining for Ki67 had a sensitivity of 97% and a specificity of 33% for differentiating CIN lesions in colposcopic biopsy specimens (p=0.001 for both). Concurrent positive staining for p16 and Ki67 in colposcopic biopsy specimens had a sensitivity of 91% and a specificity of 94% for CIN lesions (p=0.001). Conclusions: p16/Ki-67 immunostaining applied on cervical cytology specimens can screen CIN lesions with high sensitivity and specificity in a low risk population.
A conductive sodium alginate and carboxymethyl chitosan hydrogel doped with polypyrrole for peripheral nerve regeneration

Polymer materials with electrically conductive properties have good applications in their respective fields because of their special properties. However, they usually exhibited poor mechanical properties and biocompatibility. In this work, we present a simple approach to prepare conductive sodium alginate (SA) and carboxymethyl chitosan (CMCS) polymer hydrogels (SA/CMCS/PPy) that can provide sufficient help for peripheral nerve regeneration. SA/CMCS hydrogel was cross-linked by calcium ions provided by the sustained release system consisting of d-glucono-δ-lactone (GDL) and superfine calcium carbonate (CaCO3), and the conductivity of the hydrogel was provided by doped with polypyrrole (PPy). Gelation time, swelling ratio, porosity and Young's modulus of the conductive SA/CMCS/PPy hydrogel were adjusted by polypyrrole content, and the conductivity of it was within 2.41 × 10−5 to 8.03 × 10−3 S cm−1. The advantages of conductive hydrogels in cell growth were verified by controlling electrical stimulation of cell experiments, and the hydrogels were also used as a filling material for the nerve conduit in animal experiments. The SA/CMCS/PPy conductive hydrogel showed good biocompatibility and repair features as a bioactive biomaterial, we expect this conductive hydrogel will have a good potential in the neural tissue engineering.
Reading Assessment Awareness: A Comparative Analysis of Public and Private School ESL Teachers in Pakistan

Assessment literacy is of key importance to ensure quality education and meaningful learning process. Teaching is a conscious and clever business and the teachers who know what they are doing and why they are doing it are arguably better than those who know not. This quantitative study investigates the reading assessment awareness level of public and private ESL school teachers in Pakistan and the differences between the awareness levels of the two. For this purpose, the valid and reliable Language Assessment Knowledge Scale (henceforth LAKS) (Ozturk and Aydin, 2019) was adapted and run on 62 public and private ESL teachers using MS Forms. A descriptive and statistical analysis was run to measure the knowledge and differences between the awareness levels of public and private ESL teachers. The results of this study showed that the overall awareness level of public and private teachers was satisfactory; however, the private teachers were found to have a better awareness level about reading assessment. The findings of this study may be useful for teachers to re-evaluate their perceptions of themselves in terms of reading assessment competence to improve their classroom practices
A midwife’s exploration into how power & hierarchy influence both staff and patient safety

My experiences as a legitimate informal whistle-blower have afforded me an understanding of the dichotomy that is Trust allegiance and misplaced brand loyalty over and above both patient and staff safety, such that when poor care is spoken of as a potential or experienced from either angle, the general rule within healthcare management is not to acknowledge, reflect, mitigate and learn in order to improve, but instead to gaslight, deny and subordinate such that from a staff safety perspective they are caught between a rock and a hard place. This paper explores some of the opportunities which healthcare organizations could embrace to positively influence the effects of power and hierarchy on staff safety. Aims This paper discusses the bigger picture of maternity services safety. Methods This is a discussion piece. Findings For some healthcare staff it is preferable to remain quiet, not rock the proverbial boat, and maintain deeply loyal allegiances to their employers over and above public protection. For others, the journey of honesty, integrity and tenacity carries a high price in terms of personal energy, health and financial compromise. Conclusion This exploration into how power & hierarchy influence both staff and patient safety has identified and briefly explored some of the tensions created by misplaced brand loyalty inherent within healthcare institutions, and the legacy of harms resulting.
Prevalence and Antibiotic Resistance Mechanisms of Methicillin-Resistant Staphylococcus aureus isolates from Selected Hospitals in Ashanti Region, Ghana.

Background: Antibiotic resistance in bacteria has long been recognized as a major health problem occurring worldwide. The emergence of methicillin-resistant Staphylococcus aureus (MRSA) remains a global health problem. MRSA is reported as one of the leading pathogens involved in increased rates of morbidity and mortality amongst patients in Ghana. This study determined the prevalence and resistance mechanisms of MRSA isolated from patients in selected hospitals in the Ashanti Region of Ghana. Antibiograms of the isolates were determined using the Kirby-Bauer disk diffusion method. Antibiotic resistance genes (mecA and mecC) were detected and efflux pump activity assessed using polymerase chain reaction (PCR) and microbroth dilution methods, respectively. Results: Out of 626 samples obtained from patients, S. aureus was identified in 68, representing 10.9%. Multidrug resistance (MDR) was observed in 46 (67.6%) of the S. aureus isolates of which 28 (60.9%) were Methicillin Resistant Staphylococcus aureus (MRSA). The MRSA isolates showed higher susceptibility to trimethoprim-sulfamethoxazole (50%) and higher resistance to oxacillin and cefoxitin (100%). mecA gene was identified in 9 (32.1%) of the MRSA isolates whereas mecC gene was absent in all the isolates. The isolates did not exhibit any multiple efflux pump activities. Conclusion: The prevalence of MDR-MRSA in S. aureus infections at healthcare facilities in the Ashanti region was found to be high. The presence of the mecA gene was identified as a possible mechanism responsible for resistance in the MRSA isolates.
Increasing Participation in Psychological Science by Using Course-Based Research Projects: Testing Theory, Using Open-Science Practices, and Professionally Presenting Research

Introduction: Undergraduate research experiences prepare students for graduate training or employment. Statement of problem: At many teaching-intensive universities, there is a greater demand for research experiences than there are independent study opportunities. Students from typically underrepresented backgrounds may also be unaware of a department’s undergraduate research pipeline of independent study, honor’s theses, and internal research funding. Literature review: Course-based research contributes to diversity and inclusivity in access to undergraduate research experiences, especially at teaching-intensive universities. Course-based research is often integrated into methods courses, but not content courses. Teaching implications: I present practical ways to integrate research projects into courses. I stress the importance of testing theory, teaching open-science practices, and providing opportunities for students to present professionally. I also provide examples of implementing group research projects in content courses. Conclusion: Implementing course-based research projects, especially at teaching-intensive universities, can expand access to psychological science by providing valuable research opportunities for many students. Instructors can also benefit by intertwining their teaching, mentoring, and research goals. Future research should focus on experimentally testing learning outcomes.
The impact of Mergers and Acquisitions on the Financial Performance of Ecobank Ghana Limited

Methodology: The study adopted a descriptive research model with quantitative analysis. Financial ratios over a 12-year period (20062018, excluding 2012) were extracted from financial statements of the bank. The inferential and descriptive statistics were employed for the data analysis. The hypothesis of the study was tested by employing the independent sample test. Also, the Levene’s test was employed to test for the variance homogeneity.
Patient-Reported Experiences with Dialysis Care and Provider Visit Frequency

Visual Abstract Background and objectives New payment models resulting from the Advancing American Kidney Health initiative may create incentives for nephrologists to focus less on face-to-face in-center hemodialysis visits. This study aimed to understand whether more frequent nephrology practitioner dialysis visits improved patient experience and could help inform future policy. Design, setting, participants, & measurements In a cross-sectional study of patients receiving dialysis from April 1, 2015 through January 31, 2016, we linked patient records from a national kidney failure registry to patient experience data from the In-Center Hemodialysis Consumer Assessment of Healthcare Providers and Systems survey. We used a multivariable mixed effects linear regression model to examine the association between nephrology practitioner visit frequency and patient-reported experiences with nephrologist care. Results Among 5125 US dialysis facilities, 2981 (58%) had ≥30 In-Center Hemodialysis Consumer Assessment of Healthcare Providers and Systems surveys completed between April 2015 and January 2016, and 243,324 patients receiving care within these facilities had Medicare Parts A/B coverage. Face-to-face practitioner visits per month were 71% with four or more visits, 17% with two to three visits, 4% with one visit, and 8% with no visits. Each 10% absolute greater proportion of patients seen by their nephrology practitioner(s) four or more times per month was associated with a modestly but statistically significant lower score of patient experience with nephrologist care by −0.3 points (95% confidence interval, −0.5 to −0.1) and no effect on experience with other domains of dialysis care. Conclusions In an analysis of patient experiences at the dialysis facility level, frequent nephrology practitioner visits to facilities where patients undergo outpatient hemodialysis were not associated with better patient experiences.
How mycorrhizas can help forests to cope with ongoing climate change?

The ongoing climate change have multi-faceted effects not only on metabolism of plants, but also on the soil properties and mycorrhizal fungal community. Under climate change the stability of the entire forest ecosystems and the carbon balance depend to a large degree on the interactions between trees and mycorrhizal fungi. The main drivers of climate change are CO2 enrichment, temperature rise, altered precipitation patterns, increased N deposition, soil acidification and pollutants, ecosystem fragmentation and habitat loss, and biotic invasion. These drivers can impact mycorrhizal community directly and indirectly. We discussed the influence of each driver on mycorrhizal community and outlined how mycorrhizas play an important role in the resilience and recovery of forest ecosystems under climate change, by mitigating detrimental effects of CO2 enrichment, temperature rise, drought, lack of nutrients, soil acidification, pollutants, pests, and diseases. Conservation of the overall biodiversity in forest ecosystems as well as providing the most favourable conditions for the development of mycorrhizae can contribute to increasing the resilience of forest ecosystems to climate change.
The Influence of Leadership Style, Organizational Culture, and Job Satisfaction on Employee Performance Department of Education and Culture of Yapen Islands

The Department of Education and Culture of the Yapen Islands Regency as an extension of the local government in dealing with education has attempted to provide maximum public services. However, in its implementation, there are several obstacles, including not optimal services provided due to less-than-optimal employee performance. The objective of the study is to analyze the influence of leadership style, organizational culture, and job satisfaction on the performance of the employees of the Department of Education and Culture of the Yapen Islands Regency. Collecting data used a questionnaire. The research sample was calculated using the Slovin formula and obtained a total sample of 60 employees of the Department of Education and Culture of the Yapen Islands Regency. The data were tested with research instrument tests, classical assumption tests, and multiple linear regression tests using SPSS software version 25. The results showed that 1) leadership style had a positive and significant effect on employee performance, 2) organizational culture had a positive and significant effect on the performance of employees, 3) job satisfaction has no effect on employee performance
MOLLUSCICIDAL ACTIVITY OF ZINGIBER OFFICINALE AND CARICA PAPAYA ON THE EGG HATCHABILITY OF THE GOLDEN APPLE SNAIL, POMACEA CANALICULATA (GASTROPODA: AMPULIRIIDAE)

The study was conducted to assess the effect of Zingiber officinale and Carica papaya extract on the egg hatchability of the golden apple snail, Pomacea canaliculata. This is a freshwater snail that is considered a pest in agriculture as well as natural ecosystems. In combating the population of the aforementioned pest, this study evaluates the use of plant extracts such as biomolluscicides to control the production of new hatchlings from the clusters of eggs. The egg clusters were exposed to different concentrations of Z. officinale ethanolic extract and C. papaya methanolic extracts (25 ppm, 50 ppm, 100 ppm) using the direct single spraying method. Observation was made for a maximum of 30 days. The time taken for the eggs to hatch and the number of eggs successfully hatched were measured to reflect the potential of both extracts in affecting egg hatchability. The finding shows that the eggs hatch later when exposed to increasing concentrations (25 to 100 ppm) of Z. officinale extract. The number of eggs successfully hatched was significantly decreased from 86% to 9% after exposure to 25 ppm and above. Meanwhile, the extract of C. papaya did not show any significant effect on both parameters for egg hatchability of P. canaliculata, regardless of the increase in concentration from 25 to 100 ppm. Our findings reveal that the extract of Z. officinale contains higher molluscicidal activity than the extract of C. papaya. Therefore, it has the potential to be commercialized as a biomolluscicide to control the population of golden apple snails specifically at the egg stage.
Effects of Early-Stage Blood Pressure Variability on the Functional Outcome in Acute Ischemic Stroke Patients With Symptomatic Intracranial Artery Stenosis or Occlusion Receiving Intravenous Thrombolysis

Background Studies exploring the relationship between blood pressure (BP) fluctuations and outcome in acute ischemic stroke (AIS) patients treated with intravenous thrombolysis (IVT) are limited. We aimed to investigate the influence of blood pressure variability (BPV) during the first 24 h after IVT on early neurological deterioration (END) and 3-month outcome after IVT in terms of different stroke subtypes. Methods Clinical data from consecutive AIS patients who received IVT were retrospectively analyzed. The hourly systolic BP of all patients were recorded during the first 24 h following IVT. We calculated three systolic BPV parameters, including coefficient of variability (CV), standard deviation of mean BP (SD) and successive variation (SV), within the first 6, 12, and 24 h after IVT. END was defined as neurological deterioration with an increase in the National Institutes of Health Stroke Scale (NIHSS) score ≥ 4 points within the first 72 h after admission. Follow-up was performed at 90 days after onset, and favorable and poor outcomes were defined as a modified Rankin Scale scores (mRS) of ≤1 or ≥2, respectively. Results A total of 339 patients, which were divided into those with (intracranial artery stenosis or occlusion group, SIASO group) and without (non-SIASO group) SIASO, were included. Among them, 110 patients (32.4%) were with SIASO. Patients in SIASO group had higher NIHSS on admission and difference in term of mRS at 90 days compared with non-SIASO group (P < 0.001). In SIASO group, patients in favorable outcome group were younger and had lower NIHSS on admission, lower SV-24 h (14.5 ± 4.3 vs. 11.8 ± 3.2, respectively) and lower SD-24 h (12.7 ± 3.8 vs. 10.9 ± 3.3, respectively), compared with patients with poor outcome (all P < 0.05). In the multivariable logistic regression analysis, compared with the lowest SV (SV < 25% quartile), SV50−75% [odds ratio (OR) = 4.449, 95% confidence interval (CI) = 1.231–16.075, P = 0.023] and SV>75% (OR = 8.676, 95% CI = 1.892–39.775, P = 0.005) were significantly associated with poor outcome at 3 months in patients with SIASO, adjusted for age, NIHSS on admission and atrial fibrillation. No BPV parameters were associated with END in SIASO group. In non-SIASO group, there were no significant association between BPV patterns and END or 90-day outcome. Conclusions SV-24 h had a negative relationship with 3-month outcome in AIS patients with SIASO treated with IVT, indicating that BPV may affect the outcome of AIS.
Keep talented Employees onboard by Work Engagement: Dark behaviors (Blame Attributions, bullying and Turnover Intention) in Organization

Every organization has a dark side indeed and the present study aimed to explore dark side behaviors in higher education institutes from the perspective of blame attribution, workplace bullying, and turnover intention which needed to be recognized. These behaviors considered being deviant behavior affects teachers both emotionally and physically. Current study aimed to explore the concept of keeping intact the talented teacher through work engagement while battling with dark behaviors. Study further analyzed the mediating role of work engagement in the relationship between workplace bullying and turnover intention among teachers of HEIs. Quantitative research with the use of survey design and self-administered questionnaire was conducted among upon a sample of purposively selected teachers from Higher Education Institutes (HEIs) in Sargodha, Pakistan, was carried out during March to July 2019. Utrecht Work Engagement Scale (Schaufeli, et al., 2002), Blame attribution scale (Gudjonsson & Singh, 1989), Negative Act Questionnaire (Einarsen, Raknes & Helleosy, 1994), and Turnover Intention scale (Mitchell, et al., 2001) were used to measure the constructs of study. Regression analysis reveals work engagement was a significant predictor for blame attribution, workplace bullying, and turnover intention. Findings also revealed that work engagement was a key booster to keep talented employees on board while tackling workplace bullying and turnover intention. Findings of the study were helpful for organizations to keep their talented employees on board and for employees to cope with dark behaviors. sample of 223 were participated in the present correlational this their Results indicated that workplace bullying was significant predictor for work engagement (β = -.22, SE= .10, 95%CI {-.42, -.02}, P=.028), and that work engagement was significant predictor for turnover intention ( β =-.03, SE= .01, 95%CI {-.04, -.00}, P= .003). Results shows that workplace engagement was significant predictor of turnover intention after controlling for the mediator, work engagement (β =.03, SE= .01, 95%CI {.01, .05}, P= .011), consistent with mediation. Approximately, 12 % of the variance in turnover intention was accounted for by the predictors (R 2 = .12). The indirect effect was tested by using a percentile bootstrap estimation approach with the 10000 samples (Shrout & Bogler, 2002), and implemented with the Process Macro Version 3 (Hayes, 2017). These above results indicated that indirect coefficient was significant, β =. 01, SE = . 00, 95%CI {. 00, . 01}. Workplace bullying was associated with the turnover intention that was approximately . 01 points lower as mediated by work engagement.
Risk Factors For Kidney Damage In COVID-19 Patients Admitted To The Intensive Care Unit

Background — Acute kidney injury (AKI) reaches 29% in the intensive care unit (ICU). Our study aimed to determine the prevalence, features, and the main AKI factors in critically ill patients with coronavirus disease 2019 (COVID-19). Material and Methods — The study included 37 patients with COVID-19. We analyzed the total blood count test results, biochemical profile panel, coagulation tests, and urine samples. We finally estimated the markers of kidney damage and mortality. Result — All patients in ICU had proteinuria, and 80.5% of patients had hematuria. AKI was observed in 45.9% of patients. Independent risk factors were age more than 60 years, increased C-reactive protein (CRP) level, and decreased platelet count. Conclusion — Kidney damage was observed in most critically ill patients with COVID-19. The independent risk factors for AKI in critically ill patients were elderly age, a cytokine response with a high CRP level.
Visitor Counting and Monitoring in Forests Using Camera Traps: A Case Study from Bavaria (Southern Germany)

A variety of counting methods exist to analyze visitor numbers of outdoor settings such as national parks, recreation areas and urban green spaces, with sensor-based approaches being the most frequently applied. In this paper, we describe the application and practicality of camera traps originally designed for wildlife monitoring for visitor management purposes. The focus of the work is on the practicality of trigger camera traps and data collection for visitor monitoring from a more practice- and management-oriented perspective. Camera traps can provide interesting in-depth and detailed information about recreationists and are flexible and suitable for various uses; however, assessing the visual data manually requires significant staff and working time. To deal with the large amounts of data gathered for numbers of passersby and recreation activities, correlation factors between passersby and pictures were determined, so that the number of passersby related to the number of pictures taken per day or per other time unit could be established. In focusing on using the camera traps and assessing the generated data, it became clear that more studies have to be conducted to compare different methods of visitor monitoring and their accuracy in different outdoor environments.