Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptional and metabolic adjustments in AGPase deficient bt2 maize kernels

During the cloning of monogenic recessive mutations responsible for defective kernel phenotypes in a Mutator induced maize mutant collection, we isolated a new mutant allele in Brittle2 ( Bt2 ), which codes for the small subunit of ADP-glucose pyrophosphorylase (AGPase), a key enzyme in starch synthesis. RT-PCR experiments with gene-specific primers confirmed a predominant expression of Bt2 in endosperm, of Agpsemzm in embryo and of Agpslzm in leaf, but also revealed considerable additional expression in various tissues for all three genes. Bt2a , the classical transcript coding for a cytoplasmic isoform, was almost exclusively expressed in the developing endosperm, while Bt2b , an alternative transcript coding for a plastidial isoform, was expressed in almost all tissues tested with a pattern very similar to that of Agpslzm . The phenotypic analysis showed that at 30 days after pollination (DAP) mutant kernels were plumper than wildtype ones, that the onset of kernel collapse took place between 31 and 35 DAP and that the number of starch grains was greatly reduced in the mutant endosperm but not the mutant embryo. A comparative transcriptome analysis of wildtype and bt2-H2328 kernels at mid-development (35 DAP) with the 18K GeneChip® Maize Genome Array led the conclusion that the lack of Bt2 encoded AGPase triggers large scale changes on the transcriptional level that concern mainly genes involved in carbohydrate or amino acid metabolic pathways. Principal component analysis (PCA) of 1 H-NMR metabolic profiles confirmed the impact of the bt2-H2328 mutation on these pathways and revealed that the bt2-H2328 mutation did not affect exclusively the endosperm but also the embryo at the metabolic level. These data suggest that in the bt2-H2328 endosperms regulatory networks are activated that redirect excess carbon into alternative biosynthetic pathways (amino acid synthesis) or into other tissues (embryo). wildtype and bt2-H2328 endosperm, the first two PCA components explained 94% of total variability. PC1 with PC2 clearly separated the wildtype from the mutant samples. Examination of PC1 and PC2 loadings suggested that the difference between the wildtype and bt2-H2328 samples involved sucrose, fructose, malate and alanine. In the embryo the first two PCA components explained 90% of total variability. PC1 with PC2 clearly separated the mutant from the wildtype samples. Examination of PC1 and PC2 loadings suggested that the difference between the wildtype and mutant samples involved lactic acid, sucrose, citrate, lipids and proline. These data clearly demonstrated that the bt2-H2328 mutation did not only affect metabolite levels in the endosperm but also in the embryo.
Stimulationupon Stromal Cell-Derived Factor-1 Vesicles + Trafficking of CXCR4 into Rab11 13 and Rho Mediate Endosomal a G

CXCR4, like other G protein-coupled receptors, signals via heterotrimeric guanine nucleotide-binding proteins (G proteins) to regulate gene transcription, migration, development, growth, and transformation. We describe a formerly uncharacterized function of a G protein: a role in receptor trafﬁcking. We previously showed that CXCR4 and the TCR physically associate and form a heterodimer upon stromal cell-derived factor-1 or CXCL12 (SDF-1) stimulation in human T cells to prolong ERK activation and, thereby, lead to gene upregulation and cytokine secretion. The CXCR4–TCR heterodimers occur on the cell surface and in an intracellular compartment in response to SDF-1. Neither the intracellular compartment to which the CXCR4–TCR heterodimers localize nor the mechanism for localization has been elucidated. In this article, we characterize molecular mechanisms required for postendocytic trafﬁcking of CXCR4. Upon SDF-1 stimulation, CXCR4 localizes to Rab11 + vesicles, a recycling compartment near the microtubule organizing center and Golgi apparatus. This trafﬁcking requires the CXCR4 C-terminal tail domain but not the CXCR4 ubiquitination sites. The TCR also constitutively localizes to this Rab11 + compartment. Trafﬁcking of CXCR4 into the Rab11 + , TCR-containing endosomes requires actin polymerization. Furthermore, inhibiting Rho activation or depleting G a 13 prevented trafﬁcking of CXCR4 into the Rab11 + endosomes without hindering the ability of CXCR4 to endocytose. These results indicated that, upon SDF-1 treatment, G a 13 and Rho mediate the as a mediator of receptor trafﬁcking. Immunology CXCR4 localization to this compartment has been described. In this study, we investigated the molecular mechanisms that regulate the postendocytic of in response to SDF-1 Our results deﬁne the subcellular compartment that contains and describe a new role for G13 in regulating CXCR4 endosomal trafﬁcking.
Dalitz plot analyses of B 0 → D − D 0 K þ and B þ → ¯ D 0 D 0 K þ decays

We present Dalitz plot analyses for the decays of B mesons to D − D 0 K þ and ¯ D 0 D 0 K þ . We report the observation of the D (cid:2) s 1 ð 2700 Þ þ resonance in these two channels and obtain measurements of the mass M ð D (cid:2) s 1 ð 2700 Þ þ Þ ¼ 2699 þ 14 − 7 MeV =c 2 and of the width Γ ð D (cid:2) s 1 ð 2700 Þ þ Þ ¼ 127 þ 24 − 19 MeV, including statistical and systematic uncertainties. In addition, we observe an enhancement in the D 0 K þ invariant mass around 2350 – 2500 MeV =c 2 in both decays B 0 → D − D 0 K þ and B þ → ¯ D 0 D 0 K þ , which we are not able to interpret. The results are based on 429 fb − 1 of data containing 471 × 10 6 B ¯ B pairs collected at the ϒ ð 4 S Þ resonance with the BABAR detector at the SLAC National Accelerator Laboratory.
caudo-Morpho-Histological Changes in Vesicular Gland During Prepubertal and Pubertal Period of Buffalo Bull

The vesicular glands of twelve apparently healthy prepubertal and pubertal buffalo bull revealed that the vesicular glands were paired glands situated on caudo-dorsal aspect of urinary bladder at origin of pelvic urethra and were attached to terminal part of ductus deferens both in prepubertal and pubertal The vesicular gland in size than side in both prepubertal and pubertal The glands were compact, elongated, tubular and lobulated. The lobulation was more pronounced in pubertal than prepubertal bulls. Histo-morphologically the tubule-alveolar vesicular gland was composed of four layers. The vesicular gland function was optimised from prepubertal to pubertal bull.
The importance of incubation processes from the perspective of incubated and graduated companies

This study seeks to analyze the importance of the incubation process for the viability and development of micro and small enterprises based on the perspective of incubated and/or graduated companies, in a technological incubator linked to a Community College in Santa Catarina. With respect to its objectives, this is a study of a descriptive nature with a qualitative approach. The data collection occurred through the application of a questionnaire. Through this study, it was possible to observe that the incubator is/was of great importance in the formation and implementation of the companies, providing greater viability and continuation for these companies on the market.
Institutional Knowledge at Singapore Management University Accounting quality, stock price delay and future stock returns

are associated with signiﬁcantly higher price delay. Results are robust to using AQ as the sole measure of accounting quality. Results are also robust to measuring accounting quality by the FOG index of Li 2008, which is a measure of the readability of qualitative (non–ﬁnancial statement) information in annual reports. We ﬁnd that high delay ﬁrms have signiﬁcantly higher future stock returns of about 5 percent annually relative to low delay ﬁrms. We also ﬁnd that poor accounting quality in particular is associated with a statistically signiﬁcant annual return premium of about 7.7 percent in ﬁrms with the highest non–accounting-associated delay. These results suggest several opportunities for future research. One opportunity is to examine whether poor accounting quality is associated with a delay in bond prices. Another opportunity, along the lines of Verrecchia 1980, is to examine the types of news that are associated with greater stock price delay.
Self-Regulated Learning: An Exploratory Study (Level and Gender Difference)

Self-regulated learning as defined by Wolters, Pintrich and Karabenick (2005, P 2), is “an active, constructive process whereby learners set goals for their learning and then attempt to monitor, regulate, and control their cognition, motivation, and behavior, guided and constrained by their goals and the contextual features in the environment". The present study aimed to explore the level and gender difference among English as Foreign Language (EFL) university students of Afghanistan. The sample consisted of 180 (105 males and 75 females) students from four various public universities of Afghanistan. A questionnaire including 48 items assessing self-regulated learning of university students was administered. The findings of the study revealed that, maximum number of university students fall into the category of high level of self-regulated. No significant difference was found between male and female university students of Afghanistan in terms of their self-regulated learning.
Clinical Features and Prognostic Factor of Thoracic Postoperative Oligo-Recurrence of Non-Small-Cell Lung Cancer

Objective: The study aimed to clarify clinical features and prognostic factors of thoracic oligo-postoperative recurrences that underwent local therapy of non-small-cell lung cancer (NSCLC). Methods: From 2332 patients of resected pathological stage I – IIIA NSCLC between 2008 and 2015, a total of 542 patients in follow developed recurrence. Thoracic oligo-recurrence was de ﬁ ned as 1 – 3 loco-regional con ﬁ ned to lung lobe, hilar/mediastinal lymph nodes, bronchial stump, or chest wall. This study included 56 thoracic oligo-recurrences. Local therapy included secondary surgery, stereotactic radiotherapy, radiotherapy with a 45 Gy or higher dose, and proton radiation therapy, performed with radical intent. We retrospectively reviewed the postoperative data and performed the univariate and multivariate analysis by Kaplan-Meier methods and Cox regression models, respectively. Results: Thoracic Oligo-recurrence was identi ﬁ ed in 56(542,10.3%) patients, mainly in lung lobe(n=22,39%) and regional lymph nodes(n=19,34%). Compared with distant oligo-recurrences, more of the thoracic oligo-recurrences were II – III in pathological stage at initial surgery(p=0.002) and less were adenocarcinoma(p=0.005). The 5-year postoperative survival rate and postoperative progression-free survival rate of thoracic oligo-recurrence were 10.8% and 6.7%, respectively. Median post-recurrence survival (PRS) was 31 months, and the median postoperative progression-free survival (PR-PFS) was 17 months. Multivariate analyses revealed that time to recurrence ≥ 12 months was associated with improved PRS [odds ratio (OR) 0.74, con ﬁ dence interval (CI) 0.65 – 0.85], and regional lymph node oligo-recurrence was associated with poor PRS [OR 1.48, CI 1.38 – 1.60]. All the ﬁ ve long-term ( ≥ 5-year) progression-free survivors were with a solitary pulmonary recurrence. Conclusion: Thoracic postoperative oligo-recurrence of non-small-cell lung cancer is a limited but highly heterogeneous population, with different prognosis at different recurrence sites. Local therapy for thoracic oligo-recurrence of NSCLC achieved favourable PRS in a selected population. Pulmonary solitary oligo-recurrence may achieve a long survival time.
Inﬂation persistence in new EU member states

The Working Paper Series of the Czech National Bank (CNB) is intended to disseminate the results of the CNB’s research projects as well as the other research activities of both the staff of the CNB and collaborating outside contributor, including invited speakers. The Series aims to present original research contributions relevant to central banks. It is refereed internationally. The referee process is managed by the CNB Research Department. The working papers are circulated to stimulate discussion. The views expressed are those of the authors and do not necessarily reflect the official views of the CNB. Abstract Is inflation persistence in the new EU Member States (NMS) comparable to that in the euro area countries? We argue that persistence may not be as different between the two country groups as one might expect. We confirm that one should work carefully with the usual estimation methods when analyzing the NMS, given the scope of the convergence process they went through. We show that due to frequent breaks in inflation time series in the NMS, parametric statistical measures assuming a constant mean deliver substantially higher persistence estimates for the NMS than for the euro area countries. Employing a time-varying mean leads to the reversal of this result and suggests similar or lower inflation persistence for the NMS compared to euro area countries. Structural measures show that backward-looking behavior may be a more important component in explaining inflation dynamics in the NMS than in the euro area countries.
On Emotion’s Ability to Modulate Action Output

It is widely thought that emotional stimuli receive privileged neural status compared to their nonaffective counterparts. This prioritization, however, comes at a cost, as the neural capacity of the human brain is finite; the prioritization of any one object comes at the expense of other concurrent objects in the visual array competing for awareness (Desimone & Duncan, 1995). Despite this reality, little work has examined the functional benefit derived from the perceptual prioritization of affective information. Why do we preferentially attend to emotional faces? According to evolutionary accounts, emotions originated as adaptations towards action, helping to prepare the organism for movement (Darwin, 1872; Frijda, 1986). The current dissertation examines this from the perceptive of visual neuroscience and motor cognition. Chapters 1 and 2 examine the mechanisms involved during the perceptual prioritization of emotional content in the context of action system modulation. Chapters 3 and 4 then directly examine emotions effect on oculomotor action output. Results across the studies are discussed in the context of evolutionary theories related to biological origins of emotional expression.
Diagnosisof Viral Infectionsby MulticomponentMass Spectrometric Analysis the multicomponent analysis of urine

Metabolic profiles of urine extracts of humans with viral infections, as well as of media of virus-infected human tissue cultures, have been analyzed by non-fragmenting spectrometry compared with The spectra were then subjected to several alternative computerized statistical procedures to detect diagnostic biochemical profiles. Controlled longitudinal studies on fully informed, consenting volunteers who received sandfly fever virus demonstrate the onset of a characteristic metabolic pattern that precedes the onset of symptoms and subsides when the patients overcome the infection. spectrometry for and the identification of patients with an acute liver disorder (infectious hepatitis).
Vascular health and diffusion properties of normal appearing white matter in midlife

In this study, we perform a region of interest diffusion tensor imaging and advanced diffusion complexity analysis of normal appearing white matter to determine the impact of vascular health on these diffusivity metrics in midlife adults. 77 participants (26 black, 35 female) at year 30 visit in the Coronary Artery Risk Development in Young Adults longitudinal study were scanned with an advanced diffusion-weighted imaging and ﬂuid-attenuated inversion recovery protocol. Fractional anisotropy and non-linear diffusion complexity measures were estimated. Cumulative measures across 30 years (9 study visits) of systolic blood pressure, body mass index, glucose, smoking and cholesterol were calculated as the area under the curve from baseline up to year 30 examination. Partial correlation analyses assessed the association between cumulative vascular health measures and normal appearing white matter diffusion metrics in these participants. Midlife normal appearing white matter diffusion properties were signiﬁcantly associated ( P < 0.05) with cumulative exposure to vascular risk factors from young adulthood over the 30-year time period. Higher cumulative systolic blood pressure exposure was associated with increased complexity and decreased fractional anisotropy. Higher cumulative body mass index exposure was associated with decreased fractional anisotropy. Additionally, in the normal appearing white matter of black participants ( P < 0.05), who exhibited a higher cumulative vascular risk exposure, fractional anisotropy was lower and complexity was higher in comparison to normal appearing white matter in white participants. Higher burden of vascular risk factor exposure from young adulthood to midlife is associated with changes in the diffusion properties of normal appearing white matter in midlife. These changes which may reﬂect axonal disruption, increased inﬂammation and/or increased glial proliferation, were primarily observed in both anterior and posterior normal appearing white matter regions of the corpus callosum. These results suggest that microstructural changes in normal appearing white matter are sensitive to vascular health during young adulthood and are possibly therapeutic targets in interventions focused on preserving white matter health across life.
Preoperative HE4 Tumor Marker and Prognostic Factors in Endometrial Cancer: Review Article

: Purpose of the Review : The aim of the review is to summarize the current evidence of HE4 tumor marker as a prognostic marker of endometrial cancer since it is a novel and not yet validated marker in the management of this neoplasm. Recent Findings : HE4 has been studied as a preoperative prognosis marker and the result is encouraging as it is statistically related to unfavorable prognostic markers in endometrial cancer. Having a preoperative marker that relates with prognosis is a non invasive and cost-effective way to classify the patients before the surgery act. Summary : Clinicians could use HE4 as a prognostic marker to help them in the preoperative decision-making process. Thus, good prognostic patients with a low serum HE4 could have a less invasive surgery avoiding lymphadecnectomy which decrease the morbidity of the procedure and reducing the operative time.
Correlation of ‘the Five Times Sit To Stand Test’ With Balance and Gait in Stroke Patients

Introduction: The Five times sit to stand test is a simple, valid and reliable test to assess ability to complete sit to stand five times in a row. It requires lower extremity strength and considerable skill to generate sufficient speed of movement as it‟s a timed test. Objective of the study was to find the correlation between The Five times sit to stand test with balance and gait. Methodology: 36 stroke patients were assessed using Activities specific balance confidence scale and modified version of Emory functional ambulation profile Results: There was a weak negative correlation (-0.33, p<0.05) between 5TSST and ABC Score using Pearson correlation test. A high positive correlation (0.9, p<0.05) was achieved between the first three components of mEFAP with 5TSST time using Pearson correlation. While the obstacle course time and stair time with 5TSST time showed a moderate positive correlation (0.6,p<0.05). Conclusion: 5TSST correlated with balance and gait in stroke patients.
Production of Megacins C and Cx: Presumptive Evidence of Extrachromosomal Control

Exposure of growing cultures of Bacillus megaterium C4A- to ethidium bromide or an elevated growth temperature was found to eliminate megacin C production. Ethidium bromide resulted in a cure rate of up to 13%. Growth at 430C gave a cure rate of up to 99%. Megacin C production was lost spontaneously at a rate of 4% or less. There was a greater rate of spontaneous loss of megacin Cx production by B. megaterium 337, up to 14%. Growth at 430C resulted in a cure rate of up to 24% in this organism. Reversion to the Meg+ state by cured clones has never been demonstrated. These observations support the hypothesis that production of megacins C and Cx is plasmid mediated. Meg- clones ad- sorbed more megacin than either parent strain and were more susceptible to megacin action.
New Trends and Issues Proceedings on Humanities and Social Sciences

The high complexity of today’s manufacturing environment brings many problems with planning and managing, especially production, logistic and other key business processes. In many cases, it is quite complicating to identify the real causes of problems that enterprises face or to decide which one of them should be solved first. Especially, in the case of large enterprises, it is complicating to access expertise among all departments and employed professionals in order to solve the problems most efficiently. Our fuzzy model provides a simple tool for easy identification of the most significant problems of observed processes that cause their low performance according to the measured values of their key performance indicators. The model is based on data gained through interviews with production managers, industry experts and other professionals, and verified by real data from a model company. The results are presented in the form of case studies in this contribution.
Co-Localization of CD133/EGFR and Antiangiogenic Activity Drive the Antitumor Effect of Nimotuzumab and Radiation in Human GBM U87MG Xenografted in Mice

The targeting of CD133 radio and chemoresistant population have been suggested a crucial strategy to improve the local control disease in the treatment of brain tumors. In this study nimotuzumab, a monoclonal antibody specific to EGFR, showed no significant cytotoxic activity in vitro , neither alone nor in combination with radiotherapy against the human glioma cells U87MG assessed by MTT assays. In contrast, the co-administration of nimotuzumab and radiation significantly delayed subcutaneous tumor growth in NMRI nude mice, together with a significant antiangiogenic response. In vitro , U87MG cells formed tumor neurospheres when cultured in serum-free neural stem cell medium. Moreover, tumor neurospheres, but not adherent cells, showed an increased immunoreactivity to the neural CSC marker CD133. Immunofluorescence analysis performed to examine protein expression showed that CD133 was co-localized with the EGFR, suggesting a potential molecular mechanism by which nimotuzumab is able to target the CD133 radioresistant population in the U87MG cell line. In summary, the present study suggests that antitumor activity of nimotuzumab in combination with radiation against U87MG xenografts is mediated, at least in part, by a potent antiangiogenic response in addition to the ability of nimotuzumab to target the CD133 radioresistant population, suggesting a promising alternative to the failure of current available therapies in the treatment of glioblastoma.
Equity Crowdfunding Founder Teams: Campaign Success and Venture Failure

This paper examines whether solo founders are more likely to succeed in an initial equity crowdfunding (ECF) campaign and are subsequently less likely to fail than founder teams for a large sample of initial ECF campaigns conducted on the three largest UK platforms: Crowdcube, Seedrs and SyndicateRoom. The results show that solo founders have a lower probability of conducting successful initial ECF offerings than founder teams, and are also more likely to fail thereafter. The implication that founder teams enjoy more success is due to the fact that the quality of their human capital may likely attract professional investors who can act as a certification effect. Likewise, the monitoring role of professional investors helps to minimize moral hazard concerns and thus lowers the likelihood of failure for ECF founder teams. The results also establish that founder team human capital characteristics are significant determinants of initial ECF campaign outcomes and venture failure.