Vairimorpha (Nosema) ceranae Infection Alters Honey Bee Microbiota Composition and Sustains the Survival of Adult Honey Bees

Simple Summary The gut microbiota, in addition to the hosts and the pathogens, has become the third factor involved in gut disease developments, including honey bees. Interestingly, various studies reported positive associations between the gut bacteria and the most commonly found microsporidian pathogen instead of negative associations. To investigate the positive associations, a prebiotic that also exists in honey was added in the trials. Bees fed the prebiotics have slightly higher pathogen counts but lower mortalities. Microbiota analyses suggested that bees with the infection have a microbiota composition similar to that of bees with a longer lifespan, and the prebiotic seemed to enhance the similarities. Since microsporidia typically cause chronic infections, the positive associations may serve to sustain the host lifespans which is the optimal outcome for the pathogen that the survived bees can withstand pathogen proliferation and transmit the pathogens. Although the mechanisms underlying the associations were not revealed, this study indicated that nosema disease management in bees through changes in microbiota may shorten the lifespans or enhance both the infection and the bee population. Such results have appeared in recent field studies. More studies will be needed for the disease management using bee gut microbiota. Abstract Vairimorpha (Nosema) ceranae is the most common eukaryotic gut pathogen in honey bees. Infection is typically chronic but may result in mortality. Gut microbiota is a factor that was recently noted for gut infectious disease development. Interestingly, studies identified positive, instead of negative, associations between core bacteria of honey bee microbiota and V. ceranae infection. To investigate the effects of the positive associations, we added isomaltooligosaccharide (IMO), a prebiotic sugar also found in honey, to enhance the positive associations, and we then investigated the infection and the gut microbiota alterations using qPCR and 16S rRNA gene sequencing. We found that infected bees fed IMO had significantly higher V. ceranae spore counts but lower mortalities. In microbiota comparisons, V. ceranae infections alone significantly enhanced the overall microbiota population in the honey bee hindgut and feces; all monitored core bacteria significantly increased in the quantities but not all in the population ratios. The microbiota alterations caused by the infection were enhanced with IMO, and these alterations were similar to the differences found in bees that naturally have longer lifespans. Although our results did not clarify the causations of the positive associations between the infections and microbiota, the associations seemed to sustain the host survival and benefit the pathogen. Enhancing indigenous gut microbe to control nosema disease may result in an increment of bee populations but not the control of the pathogen. This interaction between the pathogen and microbiota potentially enhances disease transmission and avoids the social immune responses that diseased bees die prematurely to curb the disease from spreading within colonies.