Miracle-dz/UniFAR-FTUs huggingface dataset:
Revealing Nuanced Relationships Among Cognitive Test Anxiety, Motivation, and Self-Regulation Through Curvilinear Analyses

Student motivational profiles and self-regulated learning strategies are significant influences on overall academic success in university settings. Test anxiety has been repeatedly linked to maladaptive learning strategies and ineffective motivational frameworks. However, the results in the field have been inconsistent with respect to the precise interactions among these variables. This study employed anonymous responses from a group of volunteer students in a mid-sized Midwestern United States university, serving a primarily Caucasian and female population with an average age of 20 years. Adopting a curvilinear analytic design, this study attempted to examine the relationships among these common domains of inquiry into student thriving. The results of this study provide insights examining under which conditions cognitive test anxiety is most likely to be heightened or diminished. The results demonstrated that levels of test anxiety were greatest when (a) learners adopted primarily extrinsic or primarily intrinsic goal orientations, (b) academic tasks where the outcome was uncertain, (c) learners adopted passive learning strategies, and (d) learning strategies were more personally involved (as opposed to externalized study behaviors). Our results add to the field by identifying curvilinear models provide greater utility in identifying the relationships among these critical emotional and cognitive factors in academic settings. Furthermore, we advocate for employing identification and intervention strategies that recognize individually specific profiles of interactions among test anxiety, motivation, and self-regulation to promote more optimal success in supporting learners in university settings.
Control of ATRIAS in three dimensions: Walking as a forced-oscillation problem

In this article, we present a new controller for stable and robust walking control of ATRIAS, an underactuated bipedal robot designed based on the spring-loaded inverted pendulum (SLIP) model. We propose a forced-oscillation scheme for control of vertical motion, which we prove to be stable and contractive. Moreover, we prove that, through some mild assumptions, the dynamics of the system can be written in a hierarchical form that decouples the stability analyses of the horizontal and vertical directions. We leverage these properties to find a stabilizing class of functions for foot placement. The torso control is also proved to be decoupled using singular perturbation theory and is stabilized through a feedback linearization controller. We also take advantage of the proposed framework’s flexibility and extend it to include a new reflex-based uneven-terrain walking control scheme. We test the controller for various desired walking speeds (0 to 2.5 m/s), for stepping up and down unexpected obstacles (15 cm), and for high-speed walking on a random uneven terrain (up to 10 cm of step-ups and step-downs and up to 1.8 m/s). The results show successful performance of the controller and its stability and robustness against various perturbations.
Effects of simulated green turtle regrazing on seagrass abundance, growth and nutritional status in Moreton Bay, south-east Queensland, Australia

In some parts of their range, green turtles maintain grazing plots in seagrass beds by regular regrazing. The effects of simulated repeated grazing on subtropical seagrasses in Moreton Bay, Australia were investigated in a manipulative experiment over summer. Three seagrass species were subjected to two different clipping frequencies (simulating turtle cropping) and compared with unclipped controls over a 3.5-month summer period for the effects on seagrass biomass, leaf size and regrowth rates and water-soluble carbohydrate (WSC) and starch content. The order of the seagrass species' relative tolerance to simulated grazing was Halophila ovalis > Zostera capricorni > Cymodocea serrulata. Frequent regrazing of the green turtle's preferred seagrass, H. ovalis, resulted in an increase in leaf regrowth rate so that standing biomass of leaves and total plant material was maintained, suggesting an increase in productivity. Furthermore, whole-plant concentrations of WSC increased significantly in clipped H. ovalis plants relative to unclipped controls. In contrast, leaf biomass of the seagrass species less preferred by turtles, Z. capricorni and C. serrulata, decreased in response to repeated leaf removal relative to controls, despite maintenance of leaf regrowth rates. C. serrulata responded to repeated clipping with a reduction in leaf size and a decrease in rhizome WSC concentration. Z. capricorni also produced fewer and smaller new leaves. The ability of the preferred species, H. ovalis, to increase production of nutrient-rich standing crop in response to regrazing has major implications for green turtles and other seagrass grazers.
The plant endoplasmic reticulum is both receptive and responsive to pathogen effectors

The endoplasmic reticulum (ER) is the entry point to the secretory pathway and, as such, is critical for adaptive responses to biotic stress, when the demand for de novo synthesis of immunity-related proteins and signalling components increases significantly. Comprised of a network of interconnected tubules and cisternae, the architecture of the ER is highly pleomorphic and dynamic, rapidly remodelling to meet new cellular requirements. During infection with the hemi-biotrophic phytopathogen, Pseudomonas syringae pv. tomato DC3000, the ER in cells immediately adjacent to established bacterial colonies condenses into ‘knot-like’ structures, reminiscent of fenestrated sheets. Based on known temporal dynamics of pathogen effector delivery and initial bacterial multiplication, the timing of these observed morphological changes is rapid and independent of classical elicitor activation of pathogen-triggered immunity. To further investigate a role for ER reconfiguration in suppression of plant immunity we identified a conserved C-terminal tail-anchor domain in a set of pathogen effectors known to localize to the ER and used this protein topology in an in silico screen to identify putative ER-localised effectors within the effectorome of the oomycete Phytophthora infestans. Subsequent characterization of a subset of 15 candidate tail-anchored P. infestans effectors revealed that 11 localised to the ER and/or Golgi. Notably, transient expression of an ER-localised effector from the closely related oomycete, Plasmopara halstedii, reconfigured the ER network, revealing intimate association of labelled ER with perinuclear chloroplasts and clusters of chloroplasts, potentially facilitating retrograde signalling during plant defence.
Intense grazing by voles (Microtus pennsylvanicus) and its effect on habitat quality

Meadow voles (Microtus pennsylvanicus) were housed in fenced plots to simulate the effects of crowding on qualitative and quantitative changes of vegetation. Within one summer they reached density levels equivalent to 1000–1300 voles/ha and were removed from the plots during the fall. Biomass yields were estimated during fall and the following spring to assess the immediate effects of grazing and plant recovery before the new growth period. Significant reductions in yields of green biomass were registered in fall (15%) and in the following spring (52%) in the grazed plots. Dead matter was also reduced by intense grazing (> 7%). Biomass quality was estimated from measures of protein, total phenolics, and total nonstructural carbohydrates (TNC). Protein and phenolics in green biomass samples did not vary in the fall and spring collections but TNC varied significantly between grazed and ungrazed plots during both sampling periods. Intense grazing by voles during one summer of high density imposes quantitativ.
Compositional Characteristics of 10 Wild Plant Legumes from Mediterranean French Pastures

Moisture, lipid, fatty acid, protein, amino acid, ash, starch, free sugars, and cellulose concentrations in the aerial part of 10 wild plant legumes, growing in Mediterranean French pastures (Anthyllis vulneraria, Coronilla emerus, Coronilla minima, Coronilla varia, Cytisus sessilifolius, Dorycnium suffruticosum, Lotus delortii, Onobrychis saxatilis, Ononis minutissima, and Psoralea bituminosa), were determined. The dried aerial part of these plants contained 8.4-15.7% protein and 1.2-1.8% lipids. Amino acid analysis showed that proteins contained mainly glutamic acid + glutamine and aspartic acid + asparagine. The main fatty acids were palmitic and linolenic acids.
Age-Dependent Progression of SARS-CoV-2 Infection in Syrian Hamsters

In late 2019, an outbreak of a severe respiratory disease caused by an emerging coronavirus, SARS-CoV-2, resulted in high morbidity and mortality in infected humans1. Complete understanding of COVID-19, the multi-faceted disease caused by SARS-CoV-2, requires suitable small animal models, as does the development and evaluation of vaccines and antivirals2. Because age-dependent differences of COVID-19 were identified in humans3, we compared the course of SARS-CoV-2 infection in young and aged Syrian hamsters. We show that virus replication in the upper and lower respiratory tract was independent of the age of the animals. However, older hamsters exhibited more pronounced and consistent weight loss. In situ hybridization in the lungs identified viral RNA in bronchial epithelium, alveolar epithelial cells type I and II, and macrophages. Histopathology revealed clear age-dependent differences, with young hamsters launching earlier and stronger immune cell influx than aged hamsters. The latter developed conspicuous alveolar and perivascular edema, indicating vascular leakage. In contrast, we observed rapid lung recovery at day 14 after infection only in young hamsters. We propose that comparative assessment in young versus aged hamsters of SARS-CoV-2 vaccines and treatments may yield valuable information as this small-animal model appears to mirror age-dependent differences in human patients.
Studies of the Phosphate Dynamics in a Vertisol in Relation to the Yield and Nutrient Uptake of Rainfed Cotton

SUMMARY Field studies of the dynamics of transformation and availability of applied phosphorus in a Vertisol cropped with rainfed cotton showed that phosphorus application increased available phosphorous in the soil. However, 70–80% of the phosphorus was fixed when more than 40 kg P2O5 ha−1 was applied. Maximum uptake of phosphorus by cotton occurred during the active growth period 40 to 80 days after germination, as reflected in a steep decline in the labile pool of available phosphorus in the soil. Over 50% of the total inorganic phosphorus occurred as Ca-P, which was the major contributor to the labile pool available to the cotton crop. The maximum response of cotton in terms of both dry matter and seed cotton and the maximum uptake of nitrogen, phosphorus and potash occurred when phosphorus was applied at 40 kg P2O5 ha−1. Phosphorus application increased the length, spread, volume and weight of roots.
A P2rx7 passenger mutation affects the vitality and function of immune cells in P2X4ko and other transgenic mice

Among laboratory mouse strains many genes are differentially expressed in the same cell population. As consequence, gene targeting in 129-derived embryonic stem cells (ESCs) and backcrossing the modified mice onto the C57BL/6 (B6) background can introduce passenger mutations in the close proximity of the targeted gene. Here, we demonstrate that several 129-originating transgenic mice in which P2rx7-neighboring genes were targeted carry a P2rx7 passenger mutation that affects the vitality and function of T cells. By the example of P2rx4tm1Rass we demonstrate that CD4+ and CD8+ T cells derived from these mice express higher levels of P2X7 when compared to corresponding cell populations in B6-WT mice. The increased T cell sensitivity towards the P2X7 activators adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NAD+) rendered these cells more vulnerable towards NAD-induced cell death (NICD) compared to their B6-WT counterparts. The enhanced NICD sensitivity significantly affected the outcome of functional assays e.g. cytokine production and cell migration. For P2rx4tm1Rass, we demonstrate that the expression of P2X7 is diminished in several innate immune cell populations, possibly as a side effect of P2rx4 targeting, and independent of the P2rx7 passenger mutation. These results need to be considered when working with P2rx4tm1Rass mice or other 129-based transgenic strains that target P2rx7 neighboring genes and might have implications for other mouse models.
Host environmental conditions induce small fungal cell size and alter population heterogeneity in Cryptococcus neoformans

Fungal morphology significantly impacts the host response. Filamentation and tissue penetration by Candida and Aspergillus species are essential for virulence, while growth as a yeast allows the thermal dimorphic fungi Coccidiodes, Histoplasma, and Talaromyces to reside inside phagocytes and disseminate. The basidiomycete Cryptococcus neoformans exhibits an unusual yeast-to-titan transition thought to enhance pathogenicity by increasing fungal survival in the host lung and dissemination to the central nervous system. In a common laboratory strain (H99), in vitro and in vivo titan induction yields a heterogenous population including >10 μm titan cells, 5-7 μm yeast cells and 2-4 μm titanides. Previous reports have shown that titan cells are associated with enhanced virulence and the generation of aneuploid cells that facilitate stress adaptation and drug resistance, while small (>10 μm) cells are associated with increased dissemination. However, the relationship between titan cells, small cells, and titanides remains unclear. Here, we characterize titanides and small cells in H99 and three clinical isolates and show that titanides share the lipid membrane order of their titan mothers and the G0 quiescent-like DNA staining of mating spores. In addition, we show that both titanizing and non-titanizing isolates exhibit altered capsule structure and PAMP exposure over time during in vitro culture, and generate aneuploidy in vivo. Author summary The human fungal pathogen Cryptococcus neoformans causes 200,000 HIV-associated deaths each year. In the lung, Cryptococcus makes an unusual yeast-to-titan morphological switch that contributes to disease development by altering immune polarization and introducing aneuploidy underlying host stress and drug resistance. Specifically, a proportion of 5 um haploid yeast endoreduplicate and swell, converting to large (> 10 um) polyploid titan cells that can then produce genetically distinct daughter cells. We recently developed an in vitro protocol for inducing large titan cells and additionally observed a novel small “titanide” cell type. Here we investigate the nature and origin of these small cells, demonstrating that they emerge during both in vitro and in vivo mouse-passaged titan induction in the well characterised lab strain H99 and are also apparent in a titanizing clinical isolate, Zc8. We show that these titanide cells share features with titan mothers (lipid order) and with spores produced during heterothalic mating. Finally, we show that the capacity of clinical isolates to produce both titan and titanide cells impacts aneuploidy and the emergence of drug resistance in vivo.
Entraining corticocortical plasticity changes oscillatory activity in action control and inhibition

Oscillatory activity may reflect interactions between brain areas[1]. Here we tested whether inducing corticocortical plasticity in a specific set of connections changes oscillatory activity and cortico-cortical interactions and, if this is the case, whether the changes manifest in a manner that is behaviour state-dependent. We either increased or decreased the influence of activity in human ventral premotor cortex (PMv) over activity in primary motor cortex (M1) using cortico-cortical paired associative stimulation (ccPAS)[2, 3]. Before and after stimulation participants performed a Go/No-Go task. While M1 TMS pulses revealed the excitatory state of the motor system at specific time points, the electroencephalogram (EEG) revealed the evolution of oscillatory activity dynamics in the motor system over several hundreds of milliseconds before, during, and after each movement. Augmenting cortical connectivity between PMv and M1, by evoking synchronous pre- and postsynaptic activity in the PMv-M1 pathways, led to a state-dependent modulation of the causal influence of PMv over M1, and at the same time, enhanced oscillatory beta and theta rhythms in Go and No-Go trials, respectively. No changes were observed in the alpha rhythm. The plasticity induction effect was dependent on PMv-M1 stimulation order; the opposite patterns of results were observed after an equal amount of stimulation of PMv and M1 but applied in a temporal pattern that did not augment PMv’s influence over M1. These results are consistent with Hebbian principles of synaptic plasticity[4] and show that artificial manipulation of cortico-cortical connectivity produces state-dependent functional changes in the spectral fingerprints of the motor circuit.
SOX2 is required independently in both stem and differentiated cells for pituitary tumorigenesis in p27-null mice

Significance Tumor development can depend on cell intrinsic dysfunction, but, in some cases, extrinsic factors are important drivers. Here, we established a genetically tractable model, demonstrating that the same gene is relevant both cell autonomously and noncell autonomously for tumorigenesis. Deletion of p27, down-regulated in many tumors, predominantly leads to development of murine pituitary tumors. SOX2, transcriptionally derepressed in absence of P27, is important for tumorigenesis in this and other models, but little is known about its interaction. Using loss-of-function and lineage tracing approaches, we establish its regulatory interaction in vivo and show that SOX2 is required independently, both in endocrine and stem cells, to orchestrate tumorigenesis in absence of P27, establishing a powerful model to investigate mechanisms of tumor development. P27, a cell cycle inhibitor, is also able to drive repression of Sox2. This interaction plays a crucial role during development of p27−/− pituitary tumors because loss of one copy of Sox2 impairs tumorigenesis [H. Li et al., Cell Stem Cell 11, 845–852 (2012)]. However, SOX2 is expressed in both endocrine and stem cells (SCs), and its contribution to tumorigenesis in either cell type is unknown. We have thus explored the cellular origin and mechanisms underlying endocrine tumorigenesis in p27−/− pituitaries. We found that pituitary hyperplasia is associated with reduced cellular differentiation, in parallel with increased levels of SOX2 in stem and endocrine cells. Using conditional loss-of-function and lineage tracing approaches, we show that SOX2 is required cell autonomously in p27−/− endocrine cells for these to give rise to tumors, and in SCs for promotion of tumorigenesis. This is supported by studies deleting the Sox2 regulatory region 2 (Srr2), the target of P27 repressive action. Single cell transcriptomic analysis further reveals that activation of a SOX2-dependent MAPK pathway in SCs is important for tumorigenesis. Altogether, our data highlight different aspects of the role of SOX2 following loss of p27, according to cellular context, and uncover an unexpected SOX2-dependent tumor-promoting role for SCs. Our results imply that targeting SCs, in addition to tumor cells, may represent an efficient antitumoral strategy in certain contexts.
Changes in quality, antioxidant compounds and DPPH radical-scavenging activity of Rosa hybrida flowers during development

The antioxidant activity of rose petals harvested at six different stages of flower development was determined for two different-coloured cultivars, ‘Carola’ and ‘Iceberg’. Antioxidant assayed included phenolic compounds, vitamins and 1, 1-diphenyl-2-picrylhydrazyl hydrate (DPPH). Significant variation in antioxidant properties and quantity was observed at six different maturity stages, but there was no significant difference in antioxidant activity between the two cultivars. Correlation analysis indicated that total phenols, total flavonoids, vitamin C and vitamin E might be the main contributors to the antioxidant activity of rose petal extracts. DPPH radical-scavenging activity peaked in the early bloom stage, and flowers in this stage possessed the highest functional benefits of all maturity stages.
Crescimento de genótipos diplóides de bananeira submetidos ao estresse salino

Ten diploid banana genotypes (Musa spp.) were evaluated with regard to salt tolerance, abiotic stress which limits the productivity of the crop. The plants were grown during 21 days, in a nutrient solution with and without the addition of NaCl (0 and 100 mol m-3). Growth variables including leaf area, fresh and dry biomass, biomass allocation and growth rate were analysed. The experiment was implemented in a completely randomized design in factorial (10 x 2) arrangement and three replicates per treatment. Salinity caused in most genotypes reductions in almost all variables. Genotype Lidi stood out by having better adaptation to saline stress in all measured biometric variables with the ability to maintain, under stress, the fresh and dry biomass (leaf blade, stem, pseudostem + root), leaf area, and absolute growth rate among others. The relative production of dry biomass of shoots was more than 70%, characterizing this as a tolerant genotype and promising to be integrated into breeding programs. Genotypes Ouro and Tungia, in turn, showed reduction of absolute growth rate and relative dry biomass production (less than 50%) were characterized as sensitive to salinity.
Corticocortical Connections of the Rostral Forelimb Area in Rats: A Quantitative Tract-Tracing Study

The rostral forelimb area (RFA) in the rat is considered to be a premotor cortical region based primarily on its efferent projections to the primary motor cortex. The purpose of the present study was to identify corticocortical connections of RFA, and to describe the relative strength of connections with other cortical areas. This will allow us to better understand the broader cortical network in which RFA participates, and thus, determine its function in motor behavior. In the present study, the RFA of adult male Long-Evans rats (n=6) was identified using intracortical microstimulation techniques and injected with the tract tracer, biotinylated dextran amine (BDA). In post-mortem tissue, location of BDA-labeled terminal boutons and neuronal somata were plotted and superimposed on cortical field boundaries. The results demonstrated that the RFA has dense to moderate reciprocal connections with primary motor cortex, the frontal cortex medial and lateral to RFA, primary somatosensory cortex (S1), and lateral somatosensory areas. Importantly, S1 connections were dense to moderate in dysgranular zones, but sparse to negligible in granular zones. Cortical connections of RFA in rat are strikingly similar to cortical connections of the ventral premotor cortex in non-human primates, suggesting that these areas share similar functions.