Miracle-dz/UniFAR-FTUs huggingface dataset:
Mental health in adults living with arthrogryposis multiplex congenita

Little is known about the mental well‐being of adults living with arthrogryposis multiplex congenita (AMC). The objectives of this study were to determine the incidence of depression in an international population of adults with AMC and to identify variables independently associated with depression. This cross‐sectional study used independent samples t‐test and hierarchical multiple regression. The mean Hospital Anxiety and Depression Scale—depression (HADS‐D) score of our sample, which included 60 adults with AMC, was 4.0 ± 3.6, with 19% having some signs of depression. Occupation status, age, sex, physical independence, environmental factors, anxiety, and fatigue explained 52.2% of the variance in HADS‐D. The prevalence of depression in an adult sample of individuals with AMC is similar to that of the general adult population in the United States. Beyond direct interventions to ameliorate depression, rehabilitation clinicians may also consider treatments and interventions to decrease anxiety and reduce fatigue and environmental barriers.
2-years retrospective observational case-control study on survival and marginal bone loss of implants in patients with hereditary coagulopathies

Background Evaluating 2-years implant loss and marginal bone loss in patients with hereditary coagulopathies, comparing with a healthy control group. Material and Methods 37 implants in 13 patients (17 haemophilia A, 20 Von-Willebrand disease) versus 26 implants in 13 healthy patients. Data measured through Lagervall-Jansson index (after surgery, at prosthetic loading, at 2 years). Statistics: Chi-square, Haberman’s, ANOVA, Mann-Whitney-U. Significance p<0.05. Results Haemorrhagic accidents in 2 coagulopathies patients (non-statistical differences). Hereditary coagulopathies patients suffered more hepatitis (p<0.05), HIV (p<0.05) and less previous periodontitis (p<0.01). Non-statistical differences in marginal bone loss among groups. 2 implants were lost in the hereditary coagulopathies and none in the control group (non-statistical differences). Hereditary coagulopathies patients had longer (p<0.001), and narrower implants (p<0.05) placed. 43.2% external prosthetic connection in hereditary coagulopathies patients (p<0.001); change of prosthetic platform more frequent in control group (p<0.05). 2 implants lost: external connection (p<0.05). Survival rate 96.8% (hereditary coagulopathies 94.6%, control group 100%). Conclusions Implant and marginal bone loss at 2 years is similar in patients with hereditary coagulopathies and control group. Precautions should be taken on the treatment for hereditary coagulopathies patients, through prior haematological protocol. Implant loss only occurred in in a patient with Von-Willebrand´s disease. Key words:Dental implants, inherited coagulation disorders, haemophilia A/B, Von Willebrand disease, marginal bone loss.
The Influence of Age, Sex, and Socioeconomic Status on Glycemic Control Among People With Type 1 and Type 2 Diabetes in Canada: Patient-Led Longitudinal Retrospective Cross-sectional Study With Multiple Time Points of Measurement

Background Clinical guidelines for most adults with diabetes recommend maintaining hemoglobin A1c (HbA1c) levels ≤7% (≤53 mmol/mol) to avoid microvascular and macrovascular complications. People with diabetes of different ages, sexes, and socioeconomic statuses may differ in their ease of attaining this goal. Objective As a team of people with diabetes, researchers, and health professionals, we aimed to explore patterns in HbA1c results among people with type 1 or type 2 diabetes in Canada. Our research question was identified by people living with diabetes. Methods In this patient-led retrospective cross-sectional study with multiple time points of measurement, we used generalized estimating equations to analyze the associations of age, sex, and socioeconomic status with 947,543 HbA1c results collected from 2010 to 2019 among 90,770 people living with type 1 or type 2 diabetes in Canada and housed in the Canadian National Diabetes Repository. People living with diabetes reviewed and interpreted the results. Results HbA1c results ≤7.0% represented 30.5% (male people living with type 1 diabetes), 21% (female people living with type 1 diabetes), 55% (male people living with type 2 diabetes) and 59% (female people living with type 2 diabetes) of results in each subcategory. We observed higher HbA1c values during adolescence, and for people living with type 2 diabetes, among people living in lower income areas. Among those with type 1 diabetes, female people tended to have lower HbA1c levels than male people during childbearing years but higher HbA1c levels than male people during menopausal years. Team members living with diabetes confirmed that the patterns we observed reflected their own life courses and suggested that these results be communicated to health professionals and other stakeholders to improve the treatment for people living with diabetes. Conclusions A substantial proportion of people with diabetes in Canada may need additional support to reach or maintain the guideline-recommended glycemic control goals. Blood sugar management goals may be particularly challenging for people going through adolescence or menopause or those living with fewer financial resources. Health professionals should be aware of the challenging nature of glycemic management, and policy makers in Canada should provide more support for people with diabetes to live healthy lives.
Domestic Violence and Its Impact on Abortion in Iran: Evidence From a Nationally Representative Survey

The objective of this article is to assess the effect of domestic violence on abortion and investigate the mediating role of unwanted pregnancy. A secondary analysis was conducted on the National Family Survey data. This survey was a cross-sectional study conducted across Iran in 2018. The association between domestic violence and abortion was analyzed using the Partial Least Square-Structural Equation Model (PLS-SEM) with WarpPLS version 8.0. From among 1,544 married women (mean age 42.8 years) who participated in this survey, 27% (418 women) reported experiencing at-least one-lifetime of abortion. Overall, two in three women (67.3%) experienced at least one form of domestic violence. Almost half of the women with experience of abortion (49.3%) reported at least one unwanted pregnancy in their life course. The bivariate analysis showed a significant positive relationship between domestic violence and abortion, and there was a positive direct effect of domestic violence on unwanted pregnancy. Moreover, age had a negative direct and indirect effect on unwanted pregnancy and abortion. Although, the direct effect of domestic violence on abortion was not significant in the Structure Equation Model, a positive indirect effect of domestic violence on abortion through unwanted pregnancy was confirmed. The effect of unwanted pregnancy on abortion was particularly strong (β = .395, p < .01). These results have some implications for prevention of abortion through interventions against unwanted and unplanned pregnancy and domestic violence. This study makes a unique theoretical contribution to the literature through assessing the mediating role of unwanted pregnancy between domestic violence and abortion by using the SEM model.
Spatial expression patterns of genes encoding sugar sensors in leaves of C4 and C3 grasses

Abstract Background and Aims The mechanisms of sugar sensing in grasses remain elusive, especially those using C4 photosynthesis even though a large proportion of the world's agricultural crops utilize this pathway. We addressed this gap by comparing the expression of genes encoding components of sugar sensors in C3 and C4 grasses, with a focus on source tissues of C4 grasses. Given C4 plants evolved into a two-cell carbon fixation system, it was hypothesized this may have also changed how sugars were sensed. Methods For six C3 and eight C4 grasses, putative sugar sensor genes were identified for target of rapamycin (TOR), SNF1-related kinase 1 (SnRK1), hexokinase (HXK) and those involved in the metabolism of the sugar sensing metabolite trehalose-6-phosphate (T6P) using publicly available RNA deep sequencing data. For several of these grasses, expression was compared in three ways: source (leaf) versus sink (seed), along the gradient of the leaf, and bundle sheath versus mesophyll cells. Key Results No positive selection of codons associated with the evolution of C4 photosynthesis was identified in sugar sensor proteins here. Expressions of genes encoding sugar sensors were relatively ubiquitous between source and sink tissues as well as along the leaf gradient of both C4 and C3 grasses. Across C4 grasses, SnRK1β1 and TPS1 were preferentially expressed in the mesophyll and bundle sheath cells, respectively. Species-specific differences of gene expression between the two cell types were also apparent. Conclusions This comprehensive transcriptomic study provides an initial foundation for elucidating sugar-sensing genes within major C4 and C3 crops. This study provides some evidence that C4 and C3 grasses do not differ in how sugars are sensed. While sugar sensor gene expression has a degree of stability along the leaf, there are some contrasts between the mesophyll and bundle sheath cells.
Multigrid methods in convex optimization with application to structural design

This dissertation has investigated the use of multigrid methods in certain classes of optimization problems, with emphasis on structural, namely topology optimization. We have investigated the solution bound constrained optimization problems arising in discretization by the finite element method, such as elliptic variational inequalities. For these problems we have proposed a "direct" multi grid approach which is a generalization of existing multigrid methods for variational inequalities. We have proposed a nonlinear first order method as a smoother that reduces memory requirements and improves the efficiency of the resulting algorithm compared to the second order method (Newton's methods), as documented on several numerical examples. The project further investigates the use of multigrid techniques in topology optimization. Topology optimization is a very practical and efficient tool for the design of lightweight structures and has many applications, among others in automotive and aircraft industry. The project studies the employment of multigrid methods in the solution of very large linear systems with sparse symmetric positive definite matrices arising in interior point methods where, traditionally, direct techniques are used. The proposed multigrid approach proves to be more efficient than that with the direct solvers. In particular, it exhibits linear dependency of the computational effort on the problem size.
Disconjugacy Criteria for Nonselfadjoint Differential Equations of Even Order

Disconjugacy criteria have been established for linear selfadjoint differential equations of order 2n by Sternberg [4] and Ahlbrandt [1]. Such differential equations can be written in the form 1.1 where it is assumed that the coefficients are real and that Pn (x) ≠ 0. We shall be interested in nontrivial solutions v(x) of (1.1), which satisfy 1.2 for distinct points α and β. The smallest β> α such that (1.2) is satisfied nontrivially by a solution of (1.1), is denoted by μ 1(α) and called the first conjugate point of x = α with respect to (1.1). If no such conjugate point exists we write μ 1(α) = ∞, and say that (1.1) is disconjugate on [α, ∞). The principal purpose of this paper is to generalize these disconjugacy criteria to the general linear nonselfadjoint differential equation of the form 1.3
Coherent motions in a turbulent wake of an axisymmetric bluff body

Abstract The wake flow past an axisymmetric body of revolution at a diameter-based Reynolds number $Re=u_{\infty }D/\nu =5000$ is investigated via a direct numerical simulation. The study is focused on identification of coherent vortical motions and the dominant frequencies in this flow. Three dominant coherent motions are identified in the wake: the vortex shedding motion with the frequency of $St=fD/u_{\infty }=0.27$, the bubble pumping motion with $St=0.02$, and the very-low-frequency (VLF) motion originated in the very near wake of the body with the frequency $St=0.002$–$0.005$. The vortex shedding pattern is demonstrated to follow a reflectional symmetry breaking mode, whereas the vortex loops are shed alternatingly from each side of the vortex shedding plane, but are subsequently twisted and tangled, giving the resulting wake structure a helical spiraling pattern. The bubble pumping motion is confined to the recirculation region and is a result of a Görtler instability. The VLF motion is related to a stochastic destabilisation of a steady symmetric mode in the near wake and manifests itself as a slow, precessional motion of the wake barycentre. The VLF mode with $St=0.005$ is also detectable in the intermediate wake and may be associated with a low-frequency radial flapping of the shear layer.
Cell and Tissue Tropism of Brucella spp.

Brucella spp. are facultatively intracellular bacteria that can infect, survive, and multiply in various host cell types in vivo and/or in vitro. The genus Brucella has markedly expanded in recent years with the identification of novel species and hosts, which has revealed additional information about the cell and tissue tropism of these pathogens. ABSTRACT Brucella spp. are facultatively intracellular bacteria that can infect, survive, and multiply in various host cell types in vivo and/or in vitro. The genus Brucella has markedly expanded in recent years with the identification of novel species and hosts, which has revealed additional information about the cell and tissue tropism of these pathogens. Classically, Brucella spp. are considered to have tropism for organs that contain large populations of phagocytes such as lymph nodes, spleen, and liver, as well as for organs of the genital system, including the uterus, epididymis, testis, and placenta. However, experimental infections of several different cultured cell types indicate that Brucella may actually have a broader cell tropism than previously thought. Indeed, recent studies indicate that certain Brucella species in particular hosts may display a pantropic distribution in vivo. This review discusses the available knowledge on cell and tissue tropism of Brucella spp. in natural infections of various host species, as well as in experimental animal models and cultured cells.
Revenue management in restaurants: the role of customers’ suspicion of price increases

ABSTRACT The restaurant industry has yet to fully embrace revenue management (RM) in the form of peak-load pricing. Restaurateurs fear customers’ reactions to price increases could negatively impact their satisfaction and return intentions. In this study an experimental design was used to examine if the explanation provided to customers of a hypothetical restaurant that implemented peak-load menu pricing during periods of high (peak) demand would predict customers’ reactions. Reactions assessed included respondents’ suspicion of the restaurant, perceptions of pricing fairness and price-value, satisfaction, and return intentions. Additionally, the moderating effects of customers’ familiarity with RM and the direct effects of decisional control and income were assessed. Data from 409 respondents showed that higher suspicion was negatively associated with perceptions of pricing fairness and price-value, satisfaction, and return intentions during peak-demand periods (weekends). These lower return intentions, however, were also found for off-peak demand periods (weekdays) when no price increase had been implemented. The results suggest that suspicion, once activated, can lead to customer loss during both peak and off-peak demand periods, and that restaurant operators wanting to use peak-load pricing must carefully consider various factors in its implementation.
Consumers’ perceived and expected inflation in South Korea

ABSTRACT Household inflation expectations act as an important input in a central bank’s inflation-targeting monetary policy formulation. This study investigates the formation of households’ inflation expectations in South Korea based on a consumer survey conducted by the Bank of Korea. We use epidemiological models and find that households’ inflation expectations display strong and persistent overestimation bias. Furthermore, Bank of Korea’s inflation forecasts influence households’ inflation expectations. The findings suggest that the strong upward bias in households’ inflation expectations is consistent with the perceived inflation. The Bank of Korea needs to enhance its communication strategy to guide households’ inflation expectations based on its inflation targeting.
Barriers to birth preparedness and complication readiness among pregnant women in rural Ethiopia: using a mixed study design, 2020

Objective To assess birth preparedness and complication readiness (BPCR) and associated factors among pregnant women in Bachoo District, Oromia, Ethiopia. Design A mixed cross-sectional study design was employed to conduct this study. Setting A community-based cross-sectional study was done in the rural community of Bachoo District of Iluu Abbaa Boor Zone, Oromia Region, Southwest Ethiopia. Participants A total of 307 pregnant women participated in the quantitative study, 51 respondents were involved in the qualitative part. A simple random sampling technique was used to select the final respondents. Data were entered into EpiData V.3. 1 and analysed using SPSS V.22. Binary and multivariable logistic regression analysis was done. The level of statistical significance was declared at a p<0.05. Three focus group discussions and 21 in-depth interviews were conducted, and the data were analysed using thematic analysis and triangulated to support the findings of the quantitative study. Result The prevalence of BPCR was 30.6%. Being governmental employee ((adjusted OR, AOR=3.22 95% CI (1.49 to 11.79)), educational status of secondary and above ((AOR=1.9 95% CI (1.15 to 3.84)), multigravidity ((AOR=5.96, 95% CI (1.18 to 3.68)), having four or above ANC visits ((AOR=4.25 CI (1.38 to 7.84)), participating in pregnant women conference ((AOR=2.11 95% CI (1.07 to 3.78)), having good knowledgeable of obstetrics danger signs ((AOR=10.4 95% CI (5.57 to 19.60)), hearing the term BPCR ((AOR=4.36, 95% CI (1.93 to 9.82)) were among factors significantly associated with BPCR. The qualitative study also showed that poor maternal knowledge on birth preparedness and obstetric danger signs, negligence and weak support systems in the community were among the main barriers. Conclusion and recommendation This study demonstrated that the practice of BPCR in the study area was very low. Therefore, healthcare providers in the study area should strengthen BPCR knowledge through educating women the community at large.
Norovirus NS3 protein induces apoptosis through translation repression and dysregulation of BCL-2 pro-survival proteins

Norovirus infection is characterised by a rapid onset of disease and the development of debilitating symptoms including projectile vomiting and diffuse diarrhoea. Vaccines and antivirals are sorely lacking and developments in these areas are hampered by the lack of an adequate cell culture system to investigate human norovirus replication and pathogenesis. Herein, we describe how the model norovirus, Mouse norovirus (MNV), produces a viral protein, NS3, with the functional capacity to attenuate host protein translation which invokes the activation cell death via apoptosis. We show that this function of NS3 is conserved between human and mouse viruses and map the protein domain attributable to this function. Our study highlights a critical viral protein that mediates crucial activities during replication, potentially identifying NS3 as a worthy target for antiviral drug development.
An energy costly architecture of neuromodulators for human brain evolution and cognition

Humans spend more energy on the brain than any other species. However, the high energy demand cannot be fully explained by brain size scaling alone. We hypothesized that energy-demanding signaling strategies may have contributed to human cognitive development. We measured the energy distribution along signaling pathways using multimodal brain imaging and found that evolutionarily novel connections have up to 67% higher energetic costs of signaling than sensory-motor pathways. Additionally, histology, transcriptomic data, and molecular imaging independently reveal an upregulation of signaling at G-protein coupled receptors in energy-demanding regions. We found that neuromodulators are predominantly involved in complex cognition such as reading or memory processing. Our study suggests that the upregulation of neuromodulator activity, alongside increased brain size, is a crucial aspect of human brain evolution.
Culture of Saos-2 cells under hypoxic conditions stimulates rapid differentiation to an osteocyte-like stage

Few human osteocyte in vitro models exist and the differentiation of immature osteoblasts to an osteocyte stage typically takes at least 4-weeks of culture, making the study of this process challenging and time consuming. The osteosarcoma cell line Saos-2 has proved to be a useful model of human osteoblast differentiation through to a mature osteocyte-like stage. Culture under osteogenic conditions in a standard 5% CO2 and normoxic (21% O2) atmosphere results in reproducible mineralisation and acquisition of mature osteocyte markers over the expected 28-35 day culture period. In order to expedite experimental assays, we tested whether reducing available oxygen to mimic concentrations experienced by osteocytes in vivo would increase the rate of differentiation of Saos-2 cells. Cells cultured in a 5% CO2, 1% O2 atmosphere exhibited accelerated deposition of mineral, reaching near saturation by 14 days as demonstrated with the Alizarin Red and Von Kossa staining. The gene expression of the major hypoxia-induced transcription factor HIF1α and the key osteogenic transcription factor RUNX2 were both elevated under 1% O2. Early (COLA1, MEPE) and mature (PHEX, DMP1 and SOST) osteocyte markers were also upregulated earlier under hypoxic compared to normoxic growth conditions. Thus, culture under low oxygen accelerates key markers of osteocyte differentiation, resulting in a useful human osteocyte-like in vitro cell model within 14 days.