Miracle-dz/UniFAR-FTUs huggingface dataset:
The Feasibility and Acceptability of the Developing Real Incentives and Volition for Exercise (DRIVE) Program: A Pilot Study for Promoting Physical Activity in African American Women

Background. The purpose of the current article is to demonstrate how formative process evaluation was used in a pilot study to optimize the design and implementation of two motivationally targeted community-based physical activity (PA) interventions for inactive African American women. Method. Fifteen African American women (M age: 41.6 years) were randomized to a challenge-focused program targeted toward high autonomous motivation or a rewards-focused program targeted toward low autonomous motivation. The challenge-focused program targeted enjoyment and valuation of PA and a team-based positive social climate through competitive intergroup activities and team-based goals, whereas the rewards-focused program targeted PA interest, competency, and partner-based social support through a walking program, individual-based goals with financial incentives, and partner-based action-plans. Results. Feedback from participants revealed high levels of acceptability of essential elements. Average weekly attendance exceeded the a priori goal of ≥75% of members in attendance each week. External systematic observation demonstrated that session content dose was ≥93% in both programs. Facilitator-level fidelity exceeded the a priori goal of averaging ≥3 on a 4-point scale for behavioral skills, communication, autonomy support, and session content. The process evaluation also revealed areas for improvement, including facilitator-level social support and behavioral skills at the group-level. Process data collected through FitBits revealed that participants were engaged in self-monitoring PA during the 6-week programs. Conclusions. The formative process evaluation demonstrated adequate levels of feasibility and acceptability and also provided key insights into adjustments needed before proceeding with implementing the motivationally targeted group-based programs in a larger randomized study.
Antagonistic Effect of Salicylic Acid and Jasmonic Acid on the Expression of Pathogenesis-Related (PR) Protein Genes in Wounded Mature Tobacco Leaves

Salicylic acid (SA) and jasmonic acid (JA) are essential compounds in the pathogen- and wound-signaling path­ ways accompanying induced expression of acidic and basic pathogenesis-related (PR) protein genes, respectively. How­ ever, on the effect of exogenously supplied SA and JA in in­ duction of PR gene expression, conflicting results have been obtained using various plant materials at different de­ velopmental stages. There is no clear evidence on these effects in the presence of both signals at the same time. We analyzed the effect of SA on wound- and JA-induced basic PR gene expression and that of JA on SA-induced acidic PR gene expression in mature tobacco leaves. Wound-in­ duced accumulation of transcripts for all 4 basic PR genes tested was enhanced in the presence of MeJA, and in­ hibited in the presence of SA. On the other hand, expres­ sion of all 3 acidic PR genes tested was induced by SA and was inhibited by MeJA. Using antibodies raised against acidic PR-l and PR-2 proteins, these effects were confirm­ ed at the protein level. These results indicated that JA works as an inducer of basic PR genes, and also as an inhib­ itor for acidic PR genes, while SA does the opposite.
Epigenetic Silencing of miR-9 Promotes Migration and Invasion by EZH2 in Glioblastoma Cells

Glioblastoma (GBM) is the most common primary brain tumor in adults. Tumor invasion is the major reason for treatment failure and poor prognosis in GBM. Inhibiting migration and invasion has become an important therapeutic strategy for GBM treatment. Enhancer of zeste homolog 2 (EZH2) and C-X-C motif chemokine receptor 4 (CXCR4) have been determined to have important roles in the occurrence and development of tumors, but the specific relationship between EZH2 and CXCR4 expression in GBM is less well characterized. In this study, we report that EZH2 and CXCR4 were overexpressed in glioma patients. Furthermore, elevated EZH2 and CXCR4 were correlated with shorter disease-free survival. In three human GBM cell lines, EZH2 modulated the expression of miR-9, which directly targeted the oncogenic signaling of CXCR4 in GBM. The ectopic expression of miR-9 dramatically inhibited the migratory capacity of GBM cells in vitro. Taken together, our results indicate that miR-9, functioning as a tumor-suppressive miRNA in GBM, is suppressed through epigenetic silencing by EZH2. Thus, miR-9 may be an attractive target for therapeutic intervention in GBM.
Induction of settlement in crab megalopae by ambient underwater reef sound

The larvae of a number of crab species have been found to orientate and swim toward ambient underwater sound emanating from coastal settlement habitats. This current study examined whether ambient underwater sound also has the potential to trigger settlement responses in crab larvae. The effect of exposure to reef sound on the settlement behavior and time to metamorphosis (TTM) was examined in the megalopae of 5 common crab species, 3 from temperate waters and 2 from tropical waters. The megalopae of all 5 crab species showed marked changes in swimming behavior and a significant decrease in TTM when exposed to replayed ambient underwater reef sound compared with a silent (control) treatment. Megalopae exposed to sound decreased swimming activity earlier and displayed crawling behavior that was a precursor to both settlement and metamorphosis. Sound exposure decreased the median TTM by 33 h in Hemigrapsus sexdentatus and by 75 h in Grapsidae sp. 2. The consistent results among all species examined indicate that ambient underwater sound is likely to be an important settlement cue for the megalopae of many crab species. The wider ecological significance of acoustic settlement cues in crab larvae, in relation to other settlement cues and processes, now needs to be determined. Copyright 2009, Oxford University Press.
Broad Spectrum project: factors determining the quality of antibiotic use in primary care: an observational study protocol from Italy

Introduction The overuse of antibiotics is causing worldwide spread of antimicrobial resistance (AMR). Compared with other countries, Italy has both high antibiotic consumption rates and high rates of AMR. Due to the fact that around 90% of antibiotics are prescribed by general practitioners (GPs), this study aims to measure the impact of knowledge, attitudes and sociodemographic and workplace-related factors on the quality of antibiotic prescriptions filled by GPs in the Italian Region of Sardinia. Methods and analysis Knowledge, attitude, sociodemographic and workplace-related factors deemed to influence physicians prescribing behaviour will be evaluated in a cross-sectional study conducted among all GPs of the Italian Region of Sardinia (n=1200). A knowledge and attitudes questionnaire (Knowledge and Attitudes on Antibiotics and Resistance - Italian version: ITA-KAAR) accompanied by a sociodemographic form will be linked to drug prescription data reimbursed by the National Health System. European Surveillance of Antibiotic Consumption quality indicators for outpatient antibiotic use will be calculated from drug prescription records. Every GP will be deemed to have demonstrated an adequate quality of prescriptions of antibiotics if half of the indicator score plus one is better than the median of the region. A multivariate Poisson regression model with robust variance estimation will be used to evaluate the impact of the determinants of antibiotic prescriptions on the actual prescribing quality of each physician. Ethics and dissemination The project has been approved by the ethics committee of the Regional Health Trust of Sardinia (176/2019/CE, 24 September 2019). The results will be useful to inform evidence-based interventions to tackle irrational antibiotic use in the community.
Factors Affecting Lipase Flavor in Butter

Abstract In studies to determine the effect of cream quality, pasteurization temperature, and storage temperature on the development of lipase flavor in butter, raw cream, fresh and aged 24h, was divided into three fractions and heated at 72C, 85C, and 93C, respectively, for 18s. Each fraction of cream was churned into butter, which was divided and stored at −28.9C and 4.4C. Lipase flavor development was measured organoleptically, by acid degree value, and by free fatty acid concentration. Age of the cream significantly affected free fatty acid and acid degree values whereas heat treatment of the cream affected free fatty acids, acid degree values and flavor, and the temperature of storage affected free fatty acids and flavor. Correlation between free fatty acids and acid degree values was . 86. There was no correlation between flavor and either free fatty acids or acid degree values.
Genetic analysis for yield components in pearl millet

Genetic analysis was conducted for a 15-parent diailel cross, (F1s, excluding reciprocals) of pearl millet, grown in 3 different environments. The yield components studied were head weight, head length and head number per plant. For all the characters, both additive and non-additive kinds of genetic variances were present with a predominance for the latter. However, in the presence of high g x e interactions, the estimates of gene number differentiating the parents were underestimated and also, the heritabilities were underestimated. Based on the gca effects of the parents and the sca effects of the hybrids emanating from high gca parents, It has heen shown that the material presented good potentialities for line selection which might be valuable in developing hybrids as well as for developing synthetics/composites
Low-Dose Mepolizumab Effectiveness in Patients Suffering From Eosinophilic Granulomatosis With Polyangiitis

Eosinophilic granulomatosis with polyangiitis (EGPA) is a vasculitis characterized by multisystemic manifestations including asthma. Mepolizumab (300 mg/4 weeks) has recently been approved for EGPA. However, real-life data are scarce and report experiences with high doses of mepolizumab intravenously administered (750 mg/4 weeks). The aim of our study was to investigate in a real-life setting whether mepolizumab in EGPA patients at low doses would enable us 1) to control asthma symptoms, 2) to obtain oral corticosteroids (OCS) and/or immunosuppressors tapering and 3) to maintain clinical remission and avoid disease relapses. Mepolizumab (100 mg/4 weeks) was subcutaneously administered for 12 months in 18 EGPA patients with uncontrolled severe asthma. Symptoms, annual asthma exacerbation rates, OCS-sparing effects, lung function and eosinophil activation markers were monitored. The proportion of patients with clinical remission or relapse was also evaluated in month 12. A significant decrease in the annual rate of asthma exacerbations in association with significant changes in asthma control were observed. Specifically, 66.6% of the patients experienced no exacerbations during the mepolizumab treatment. Most patients (77.7%) were able to reduce the daily OCS dose by at least 50%. Four patients also stopped cyclosporine A during the study period. No EGPA relapse was observed and a large majority of the patients achieved clinical remission (94.3%). Clinical benefits were paralleled by reduction in blood eosinophils and serum levels of eosinophil activation markers. Low-dose mepolizumab showed clinically relevant benefits in exacerbation rates, asthma symptoms, OCS and immunosuppressive use in EGPA patients. These effects occurred without any EGPA relapse for extrapulmonary manifestations.
Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers

DNA-sequence data from the mitochondrial genome are being used with increasing frequency to estimate phylogenetic relationships among animal taxa. The advantage to using DNA-sequence data is that many of the processes governing the evolution and inheritance of DNA are already understood. DNA data, however, do not guarantee the correct phylogenetic tree because of problems associated with shared ancestral polymorphisms and multiple substitutions at single nucleotide sites. Knowledge of evolutionary processes can be used to improve estimates of patterns of relationships and can help to assess the phylogenetic usefulness of individual genes and nucleotides. This article reviews molecular processes, discusses the correction of genetic distances and the weighting of DNA data, and provides an assessment of the phylogenetic usefulness of specific mitochondrial genes.
Isolation and antibiotic sensitivity of Escherichia coli from pregnant and non-pregnant women attending the University of Maiduguri Teaching Hospital (UMTH), Maiduguri, Nigeria

This study was carried out to isolate and determine the antimicrobial susceptibility patterns of Escherichia coli from pregnant and non-pregnant women with urinary tract infection attending University of Maiduguri Teaching Hospital (UMTH) Maiduguri, Nigeria from X- 2008.Clean voided mid-stream urine samples were collected in sterile universal bottles while high vaginal swab samples were obtained from patients with the use of sterile swab stick. A total of 260 samples were collected comprising 65urine samples each from pregnant and non-pregnant women and 65 high vaginal swabs each from pregnant and non-pregnant women. The results showed that out of the sixty-five (65) urine samples collected from pregnant women attending the health institutions, 48 (73.9%) were positive for various species of bacteria which includes Escherichia coli 26 (40.0%), Staphylococcus aureus 10 (15.4%), Proteus species (15.4%) and Klebsiella species 2 (3.1%). Out of the 65 high vaginal swab samples collected from the pregnant women, 16 (24.6%) were positive for various species of bacteria among which are Escherichia coli 7 (10.8%) , Staphylococcus aureus 6 (9.2%), Proteus species 3 (4.6%). Out of the sixty-five (65) urine samples collected from non-pregnant women attending the health institutions, 33(50.8%) were positive for various species of bacteria which includes Escherichia coli 15 (23.1%), Staphylococcus aureus 10 (15.4%) and Proteus species 8 (12.3%). Out of the 65 high vaginal samples collected from non-pregnant women attending the health institutions 17 (26.1%) were positive for the following species of bacteria, Escherichia coli 6 (9.2%), Staphylococcus aureus 5 (7.7%) and Proteus species 6 (9.2%). The result of antibiotic sensitivity test on E.coli isolates showed that they are highly sensitive to the quinolone group of antibiotics such as Ciprofloxacin, Ceporex and Perflacin. The organism is intermediate sensitive to Streptomycin, Tarivid and Gentamycin but resistant to Augmentin, Nalidixic acid, Septrin and Ampicillin.
Influence of Age on Partial Clinical Remission among Children with Newly Diagnosed Type 1 Diabetes

Partial clinical remission (PCR) is a transitory period characterized by the residual endogenous insulin secretion following type 1 diabetes (T1D) diagnosis and introducing the insulin therapy. Scientific interest in PCR has been recently increasing, as this phase could be crucial to preserve functional beta cells after T1D onset, also taking advantage of new therapeutic opportunities. The aim of this study was to assess the frequency, duration and associated factors of PCR in children newly diagnosed with T1D. Our cohort study included 167 pediatric patients aged 13.8 ± 4.1 years. The association of clinical and laboratory factors with the occurrence and duration of PCR was evaluated via logistic regression and multivariable generalized linear model, respectively. PCR occurred in 63.5% of the examined patients. Patients who achieved the remission phase were significantly older, and they had lower daily insulin requirement compared with non-remitters. PCR was positively associated to body mass index (OR = 1.11; p = 0.032), pH value (OR 49.02; p = 0.003) and c-peptide levels (OR 12.8; p = 0.002). The average duration of PCR was 13.4 months, and older age at diagnosis was the only predictor factor. Two years after diagnosis remitter patients had lower HbA1c and daily insulin requirement.
Talent Management, Affective Organizational Commitment and Service Performance in Local Government

Talent management (TM) is a fundamental issue for both private and public sector companies. This study analyzes the impact of TM on service performance (SP) and the mediating role of affective organizational commitment (AOC). We analyze a sample of 104 local government employees with three measures of TM, AOC and SP. The mediation hypothesis of AOC was also raised in the study using Baron and Kenny’s methodology and Hayes PROCESS. The results reveal how AOC is a total mediating variable in the causal relationship TM → SP. This study is cross-sectional. Common-method bias is controlled in the study. The results involves a concern for improving services through the professionals who provide them, which in turn entails managing people in a way that is different, more flexible, less bureaucratic, and more client- or citizen-oriented. Given the scant research exploring the role of talent management in public services, this article offers valuable insights for scientific literature and practitioners in the public administration.
Revision of the Australian ground-beetle genus Tasmanitachoides Erwin (Insecta : Coleoptera : Carabidae : Bembidiinae), with special regard to the tropical species

The Australian Tachyine genus Tasmanitachoides Erwin is revised, and the following new species are described: T. angulicollis, sp. nov., T. maior, sp nov., T. minor, sp. nov., T. bicolor, sp. nov. and T. rufescens, sp. nov. Lectotypes and paralectotypes are designated for T. leai (Sloane) and T. obliquiceps (Sloane), and T. wilsoni (Sloane) and T. obliquiceps (Sloane), not mentioned by earlier revisors of the genus, are included in Tasmanitachoides. From a short evaluation of the phylogenetic status of the species, it appears that the generalised groups of species are still living in south-eastern Australia, including Tasmania, whereas most species of the more derivative species group have spread over tropical northern Australia, and are still in a period of rapid evolution.
Transgenic assays for the analysis of DNA repair in plants

In this work we studied various aspects of DNA repair in plants, focusing mainly on point mutation repair and its interconnection with double-strand break repair. We were using transgenic point mutation and recombination substrates as a primary tool in our experiments. We have compared two transgenic homologous recombination assays (J3glucuronidaseand luciferase-based), analyzed the sensitivity of DNA repair machinery to ultraviolet radiation and assessed the involvement of AtKu80, Atm and AtXpd repair genes in point mutation repair. Our study revealed the following: the luciferase-based recombination assay is more sensitive then ft-glucuronidase-based; double-strand break repair machinery is sensitive to ultraviolet radiation, which results in increased point mutation formation; chosen DNA repair genes might be impaired in point mutation repair, however further experimentations are needed to confirm this.
Micropropagation of Astragalus Maximus Willd

ABSTRACT Micropropagation of Astragalus maximus Willd. through axillary bud culture was achieved 30 day-old plants. The shoots of plants were used as explants for micropropagation. The explants were cultured on MS media supplemented with BA (0.2, 0.5, 1.0 mg/l), ZR (0.2, 0.5, 1.0 mg/l) and their combinations with 0.4 mg/l NAA. After 20 days, micropropagation was strongly induced in MS medium supplemented with 0.5 mg/l ZR, ∼30 shoots obtained from each explant. In all other treatments, proliferation number on per explant varied to 2–3. Propagated shoots were cultured on MS medium without plant growth regulators, MS medium supplemented with 0.2–1.5 mg/l NAA and ½ MS with 1.5 % sucrose. Root induction was only achieved on ½ MS containing 1.5 % sucrose after 20 days. Plane MS and MS medium with 0.2, 0.4, 0.8, 1.2 and 1.5 mg/l NAA did not induce root formation.
Drug scheduling by nurses and drug interactions in patients with cardiovascular diseases.

OBJECTIVES to identify and characterize the potential serious drug interactions in patients hospitalized with cardiovascular diseases, relating them to the schedules established for drug administration by nurses. METHODS a documentary, quantitative and sectional research. Ninety-nine prescriptions from patients admitted to the cardiology ward of a hospital in Rio de Janeiro for more than 48 hours were analyzed. Drug interaction was assessed using the Micromedex® software. The data were analyzed using descriptive and inferential statistics. RESULTS serious interactions were evidenced in 22 drug pairs, most frequently at 6 p.m. and 6 a.m., times with higher dose scheduling performed by nurses. The most recurrent drug pairs involved in serious interactions were simvastatin + amlodipine and enoxaparin + clopidogrel. CONCLUSIONS drug scheduling by nurses requires a review of the criteria for proposing schedules for drugs in order to ensure patient safety.
Emerging Roles for Folate and Related B-Vitamins in Brain Health across the Lifecycle

Nutrition plays a fundamental role in supporting the structural and functional development of the human brain from conception, throughout early infancy and extending into later life. A growing body of evidence suggests that folate and the metabolically related B-vitamins are essential for brain health across all age groups, owing to their specific roles in C1 metabolism and particularly in the production of S-adenosylmethionine, a universal methyl donor essential for the production of neurotransmitters. Emerging, though not entirely consistent, evidence suggests that maternal folate status throughout pregnancy may influence neurodevelopment and behaviour of the offspring. Furthermore optimal B-vitamin status is associated with better cognitive health in ageing. Of note, a recent clinical trial provided evidence that supplementation with folic acid and related B-vitamins over a 2-year-period reduced global and regional brain atrophy, as measured by MRI scan in older adults. In terms of potential mechanisms, the effects of these B-vitamins on cognitive health may be independent or may be mediated by nutrient- nutrient and/or relevant gene-nutrient interactions. Furthermore, a new area of research suggests that the in utero environment influences health in later life. Folate, an important cofactor in C1 metabolism, is indirectly involved in DNA methylation, which in turn is considered to be one of the epigenetic mechanisms that may underlie fetal programming and brain development. The present review will explore the evidence that supports a role for folate and the related B-vitamins in brain health across the lifecycle, and potential mechanisms to explain such effects.
INCIDENCE AND TREATMENT OF HAEMONCHOSIS AND IMMATURE AMPHISTOMOSIS IN NATIVE GOAT BREEDS

Incidence of haemonchosis and immature amphistomosis in native goat breeds of Thiruvarur District, Tamil Nadu irrespective of age and sex is reported. Affected animals passed semisolid and/or diarrhoeic faeces with mucous and digested blood. All affected goats were anaemic. Immature amphistomes were observed on gross examination of faecal samples. Necropsy findings revealed pin point haemorrhages and adult Haemonchus spp. worms in the abomasums. Anthelmintics such as mebendazole, albendazole, fenbendazole and oxyclozanide were found to be ineffective in controlling haemonchosis indicating the development of resistance to these anthelmintics in the study area.