Miracle-dz/UniFAR-FTUs huggingface dataset:
Pupils’ Use of Social Media and Its Relation to Mental Health from a School Personnel Perspective: A Preliminary Qualitative Study

The extent of mental health problems among adolescents seems to be on the rise, and this observed trend has often been linked to a coinciding increase in social media use. The goal of the current preliminary study was to investigate how senior high school personnel experience the role of social media in relation to the mental health of their pupils. Two focus group interviews (total n = 11) were completed and analyzed using reflexive thematic analysis, resulting in 4 themes and 11 subthemes. The results illustrate that school personnel experience social media as a tool for communication, but also as a potential cause of mental health issues and reduced academic performance among pupils. The participants called for schools to become better equipped to meet the opportunities and challenges of social media.
Modification of Diurnal Cortisol Secretion in Women’s Professional Basketball. A Pilot Study

Elite basketball training causes high levels of physiological stress, which can lead to negative physiological disorders in female athletes. The aim of this study was to establish the impact of physical activity on the rhythm of salivary cortisol secretion in elite female basketball players over one week. The population sample included 9 women professional basketball players. The control group was made up of 9 women who did not do any exercise. Saliva samples were collected from all participants at 9:00 a.m. and 11:00 p.m. on training days. Samples from the basketball group showed a significantly higher cortisol secretion. Moreover, from the second night, the pattern of cortisol secretion of these players was reversed, showing higher levels of cortisol in saliva at night than in the morning. The results suggest that the secretion rhythm changed over the course of the week and according to competitive demands.
Serum Proteomic Analysis of Cannabis Use Disorder in Male Patients

Cannabis use has been growing recently and it is legally consumed in many countries. Cannabis has a variety of phytochemicals including cannabinoids, which might impair the peripheral systems responses affecting inflammatory and immunological pathways. However, the exact signaling pathways that induce these effects need further understanding. The objective of this study is to investigate the serum proteomic profiling in patients diagnosed with cannabis use disorder (CUD) as compared with healthy control subjects. The novelty of our study is to highlight the differentially changes proteins in the serum of CUD patients. Certain proteins can be targeted in the future to attenuate the toxicological effects of cannabis. Blood samples were collected from 20 male individuals: 10 healthy controls and 10 CUD patients. An untargeted proteomic technique employing two-dimensional difference in gel electrophoresis coupled with mass spectrometry was employed in this study to assess the differentially expressed proteins. The proteomic analysis identified a total of 121 proteins that showed significant changes in protein expression between CUD patients (experimental group) and healthy individuals (control group). For instance, the serum expression of inactive tyrosine protein kinase PEAK1 and tumor necrosis factor alpha-induced protein 3 were increased in CUD group. In contrast, the serum expression of transthyretin and serotransferrin were reduced in CUD group. Among these proteins, 55 proteins were significantly upregulated and 66 proteins significantly downregulated in CUD patients as compared with healthy control group. Ingenuity pathway analysis (IPA) found that these differentially expressed proteins are linked to p38MAPK, interleukin 12 complex, nuclear factor-κB, and other signaling pathways. Our work indicates that the differentially expressed serum proteins between CUD and control groups are correlated to liver X receptor/retinoid X receptor (RXR), farnesoid X receptor/RXR activation, and acute phase response signaling.
Weightlifting during the COVID-19 Pandemic—A Transnational Study Regarding Motivation, Barriers, and Coping of Master Athletes

Sport has been heavily impacted by the pandemic for over a year with restrictions and closures of facilities. The main aims of this study are to identify motivation and barriers for an international group of Master weightlifters (ages 35 and up) and analyze age and gender differences in pandemic-related changes to physical activities. A sample of 1051 older athletes, 523 women and 528 men, aged from 35 to 88 years, from Australia, Canada, Europe, and the USA provided responses to an online survey conducted in June 2021. A confirmatory factor analysis was performed to examine age, gender, and regional differences about motivation, barriers, and pandemic impact on sport and physical activities. Participants showed enthusiasm for the opportunity to compete despite health challenges with increasing age but faced barriers due to access to training facilities and qualified coaches even before the pandemic. The oldest athletes had the greatest reduction in physical activities during the pandemic. Weightlifters had the opportunity to compete in virtual competitions and 44% would like to see some of these continued in the future, especially women. These findings highlight the benefits of competitive sports and may provide future directions in strength sports for organizations, sports clubs, and coaches.
Novel Cold-Adapted Recombinant Laccase KbLcc1 from Kabatiella bupleuri G3 IBMiP as a Green Catalyst in Biotransformation

Cold-adapted enzymes are useful tools in the organic syntheses conducted in mixed aqueous-organic or non-aqueous solvents due to their molecular flexibility that stabilizes the proteins in low water activity environments. A novel psychrophilic laccase gene from Kabatiella bupleuri, G3 IBMiP, was spliced by Overlap-Extension PCR (OE-PCR) and expressed in Pichia pastoris. Purified recombinant KbLcc1 laccase has an optimal temperature of 30 °C and pH of 3.5, 5.5, 6.0, and 7.0 in the reaction with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), guaiacol, sinapic acid, and syringaldazine, respectively. Moreover, laccase KbLcc1 is highly thermolabile, as it loses 40% of activity after 30 min at 40 °C and is inactivated at 50 °C after the same period of incubation. The new enzyme remained active with 1 mM of Ni2+, Cu2+, Mn2+, and Zn2+ and with 2 mM of Co2+, Ca2+, and Mg2+, but Fe2+ greatly inhibited the laccase activity. Moreover, 1% ethanol had no impact on KbLcc1, although acetone and ethyl acetate decreased the laccase activity. The presence of hexane (40%, v/v) caused a 58% increase in activity. Laccase KbLcc1 could be applied in the decolorization of synthetic dyes and in the biotransformation of ferulic acid to vanillin. After 5 days of reaction at 20 °C, pH 3.5, with 1 mM ABTS as a mediator, the vanillin concentration was 21.9 mg/L and the molar yield of transformation reached 14.39%.
Anthocyanin Color Stabilization by Host-Guest Complexation with p-Sulfonatocalix[n]arenes

Flavylium-based compounds in their acidic and cationic form bring color to aqueous solutions, while under slightly acidic or neutral conditions they commonly bring discoloration. Selective host-guest complexation between water-soluble p-sulfonatocalix[n]arenes (SCn) macrocycles and the flavylium cationic species can increase the stability of the colored form, expanding its domain over the pH scale. The association constants between SCn and the cationic (acid) and neutral basic forms of flavylium-based compounds were determined through UV-Vis host-guest titrations at different pH values. The affinity of the hosts for synthetic chromophore was found to be higher than for a natural anthocyanin (Oenin). The higher affinity of SC4 for the synthetic flavylium was confirmed by 1H NMR showing a preferential interaction of the flavylium phenyl ring with the host cavity. In contrast with its synthetic counterpart, the flavylium substitution pattern in the anthocyanin seems to limit the inclusion of the guest in the host’s binding pocket. In this case, the higher affinity was observed for the octamer (SC8) likely due to its larger cavity and higher number of negatively charged sulfonate groups.
Rapid Production and Genetic Stability of Human Mesenchymal Progenitor Cells Derived from Human Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells

Pluripotent stem cell-derived mesenchymal progenitor cells (PSC-MPCs) are primarily derived through two main methods: three-dimensional (3D) embryoid body-platform (EB formation) and the 2D direct differentiation method. We recently established somatic cell nuclear transfer (SCNT)-PSC lines and showed their stemness. In the present study, we produced SCNT-PSC-MPCs using a novel direct differentiation method, and the characteristics, gene expression, and genetic stability of these MPCs were compared with those derived through EB formation. The recovery and purification of SCNT-PSC-Direct-MPCs were significantly accelerated compared to those of the SCNT-PSC-EB-MPCs, but both types of MPCs expressed typical surface markers and exhibited similar proliferation and differentiation potentials. Additionally, the analysis of gene expression patterns using microarrays showed very similar patterns. Moreover, array CGH analysis showed that both SCNT-PSC-Direct-MPCs and SCNT-PSC-EB-MPCs exhibited no significant differences in copy number variation (CNV) or single-nucleotide polymorphism (SNP) frequency. These results indicate that SCNT-PSC-Direct-MPCs exhibited high genetic stability even after rapid differentiation into MPCs, and the rate at which directly derived MPCs reached a sufficient number was higher than that of MPCs derived through the EB method. Therefore, we suggest that the direct method of differentiating MPCs from SCNT-PSCs can improve the efficacy of SCNT-PSCs applied to allogeneic transplantation.
Bird’s-Eye View of Sampling Sites: Using Unmanned Aerial Vehicles To Make Chemistry Fieldwork Videos

Drones, unmanned aerial vehicles (UAVs), usually helicopters or airplanes, are commonly used for warfare, aerial surveillance, and recreation. In recent years, drones have become more accessible to the public as a platform for photography. In this report, we explore the use of drones as a new technological filming tool to enhance student learning in the field of analytical and environmental chemistry. In particular, chemistry students have difficulty in applying knowledge learned in the practical context, e.g., finding potential sites for soil sampling. Our effort was focused on improving the understanding of terrain and examining potential “good” sampling sites by providing instructor’s point of view (IPOV) videos filmed with a drone. Students responded positively to this innovative filming method in a perception survey. The merits and challenges of filming with drones will be discussed.
Properties of Inorganic Polymers Based on Ground Waste Concrete Containing CuO and ZnO Nanoparticles

The effect of copper oxide and zinc oxide nanoparticles (NPs) on the mechanical and thermal properties of ground waste concrete inorganic polymers (GWC IPs) has been investigated. NPs are added to GWC IPs at loadings of 0.1, 0.5, 1, and 2% w/w. The phase composition and microstructure of NPs GWC IPs have also been examined using X-ray diffraction (XRD), Raman spectroscopy and scanning electron microscope (SEM/EDS) techniques. Results show that the mechanical properties of GWC IPs are improved (23 MPa) due to addition of NPs (1% ZnO). In particular, GWC IPs embedded with 0.5% CuO and 1% ZnO NPs exhibited relatively improved compressive strength. The addition of NPs decreases the macroporosity and increases the mesoporosity of IPs matrix and decreases relatively the ability of IPs matrix to water absorption. The antimicrobial activity of GWC IPs doped with 0.5 and 1% CuO NPs against E. coli was also determined.
Motivation Research on the Content Creation Behaviour of Young Adults in Anxiety Disorder Online Communities

With the advancements in science and technology and the improvement of medical care, mental health problems are receiving increasing attention. Increasing numbers of children, adolescents, and young adults are susceptible to anxiety. This paper assesses young adults based on self-determination theory and the theory of planned behaviour to determine the intrinsic and extrinsic motivations and mediating variables behind young adults’ content creation behaviour within anxiety disorder online communities (ADOCs). In addition, the paper introduces empathy as a moderating variable, builds a model of the content creation behavioural motivation of young adults, studies the motivation behind young adults’ content creation behaviour in ADOCs, and determines the moderating effect of empathy on young adults’ content creation behaviour. The research data were obtained using a questionnaire survey, and the SmartPLS structural equation model was used for empirical analysis. The study found that expressing one’s anxiety was the most obvious motivation, the content creation intention of young adults significantly positively affected their content creation behaviour, perceived enjoyment motivation had a significant negative influence on young adults’ intention to create content, reward motivation had no significant influence on the content creation intention of young adults, other motivations had significant positive influences on young adults’ content creation intention, and empathy only had a significant negative moderating effect on the relationship between self-efficacy and young adults’ content creation intention. This study not only enriches and expands research on motivation theory but also has practical significance for the improvement and active development of ADOCs.
Does the Rainbow Trout Ovarian Fluid Promote the Spermatozoon on Its Way to the Egg?

The fertilization of freshwater fish occurs in an environment that may negatively affect the gametes; therefore, the specific mechanisms triggering the encounters of gametes would be highly expedient. The egg and ovarian fluid are likely the major sources of these triggers, which we confirmed here for rainbow trout (Oncorhynchus mykiss). The ovarian fluid affected significantly spermatozoa performance: it supported high velocity for a longer period and changed the motility pattern from tumbling in water to straightforward moving in the ovarian fluid. Rainbow trout ovarian fluid induced a trapping chemotaxis-like effect on activated male gametes, and this effect depended on the properties of the activating medium. The interaction of the spermatozoa with the attracting agents was accompanied by the “turn-and-run” behavior involving asymmetric flagellar beating and Ca2+ concentration bursts in the bent flagellum segment, which are characteristic of the chemotactic response. Ovarian fluid created the optimal environment for rainbow trout spermatozoa performance, and the individual peculiarities of the egg (ovarian fluid)–sperm interaction reflect the specific features of the spawning process in this species.
A Longitudinal Analysis of Early Lesion Growth in Presymptomatic Patients with Cerebral Adrenoleukodystrophy

BACKGROUND AND PURPOSE: Cerebral adrenoleukodystrophy is a devastating neurological disorder caused by mutations in the ABCD1 gene. Our aim was to model and compare the growth of early cerebral lesions from longitudinal MRIs obtained in presymptomatic patients with progressive and arrested cerebral adrenoleukodystrophy using quantitative MR imaging–based lesion volumetry. MATERIALS AND METHODS: We retrospectively quantified and modeled the longitudinal growth of early cerebral lesions from 174 MRIs obtained from 36 presymptomatic male patients with cerebral adrenoleukodystrophy. Lesions were manually segmented using subject-specific lesion-intensity thresholding. Volumes were calculated and plotted across time. Lesion velocity and acceleration were calculated between sequentially paired and triplet MRIs, respectively. Linear mixed-effects models were used to assess differences in growth parameters between progressive and arrested phenotypes. RESULTS: The median patient age was 7.4 years (range, 3.9–37.0 years). Early-stage cerebral disease progression was inversely correlated with age (ρ = −0.6631, P < .001), early lesions can grow while appearing radiographically stable, lesions undergo sustained acceleration in progressive cerebral adrenoleukodystrophy (β = 0.10 mL/month2 [95% CI, 0.05−0.14 mL/month2], P < .001), and growth trajectories diverge between phenotypes in the presymptomatic time period. CONCLUSIONS: Measuring the volumetric changes in newly developing cerebral lesions across time can distinguish cerebral adrenoleukodystrophy phenotypes before symptom onset. When factored into the overall clinical presentation of a patient with a new brain lesion, quantitative MR imaging–based lesion volumetry may aid in the accurate prediction of patients eligible for therapy.
Impact of Polyethylene Glycol Functionalization of Graphene Oxide on Anticoagulation and Haemolytic Properties of Human Blood

Graphene oxide (GO) is one of the most explored nanomaterials in recent years. It has numerous biomedical applications as a nanomaterial including drug and gene delivery, contrast imaging, cancer treatment, etc. Since most of these applications need intravenous administration of graphene oxide and derivatives, the evaluation of their haemocompatibility is an essential preliminary step for any of the developed GO applications. Plentiful data show that functionalization of graphene oxide nanoparticles with polyethylene glycol (PEG) increases biocompatibility, thus allowing PEGylated GO to elicit less dramatic blood cell responses than their pristine counterparts. Therefore, in this work, we PEGylated graphene oxide nanoparticles and evaluated the effects of their PEGylation on the structure and function of human blood components, especially on the morphology and the haemolytic potential of red blood cells (RBCs). Further, we studied the effect of PEGylation on some blood coagulation factors, including plasma fibrinogen as well as on the activated partial thromboplastin (aPTT), prothrombin time (PT) and platelet aggregation. Our findings provide important information on the mechanisms through which PEGylation increases GO compatibility with human blood cells. These data are crucial for the molecular design and biomedical applications of PEGylated graphene oxide nanomaterials in the future.
DHX30 Coordinates Cytoplasmic Translation and Mitochondrial Function Contributing to Cancer Cell Survival

Simple Summary Translation occurs in the cell both through cytoplasmic and mitochondrial ribosomes, respectively translating mRNAs encoded by the nuclear and the mitochondrial genome. Here we found that the silencing of DHX30, an RNA-binding protein that we previously studied for its role in p53-dependent apoptosis, enhances the translation of mRNAs coding for cytoplasmic ribosomal proteins while reducing that of the mRNAs encoding for mitoribosomal proteins. This coordination of the cytoplasmic and mitochondrial translation machineries affected both cell proliferation and energy metabolism, suggesting an important role for this mechanism in determining the fitness of cancer cells. By integrating multiple datasets, we identified a gene signature that will represent a starting point to assess the prognostic value of this mechanism in cancer. We thus propose DHX30 as a potential vulnerability in cancer cells that could be exploited to develop novel therapeutic strategies. Abstract DHX30 was recently implicated in the translation control of mRNAs involved in p53-dependent apoptosis. Here, we show that DHX30 exhibits a more general function by integrating the activities of its cytoplasmic isoform and of the more abundant mitochondrial one. The depletion of both DHX30 isoforms in HCT116 cells leads to constitutive changes in polysome-associated mRNAs, enhancing the translation of mRNAs coding for cytoplasmic ribosomal proteins while reducing the translational efficiency of the nuclear-encoded mitoribosome mRNAs. Furthermore, the depletion of both DHX30 isoforms leads to higher global translation but slower proliferation and lower mitochondrial energy metabolism. Isoform-specific silencing supports a role for cytoplasmic DHX30 in modulating global translation. The impact on translation and proliferation was confirmed in U2OS and MCF7 cells. Exploiting RIP, eCLIP, and gene expression data, we identified fourteen mitoribosome transcripts we propose as direct DHX30 targets that can be used to explore the prognostic value of this mechanism in cancer. We propose that DHX30 contributes to cell homeostasis by coordinating ribosome biogenesis, global translation, and mitochondrial metabolism. Targeting DHX30 could, thus, expose a vulnerability in cancer cells.
Microstructure, Mechanical, and Corrosion Behavior of Al2O3 Reinforced Mg2Zn Matrix Magnesium Composites

Powder metallurgy (PM) method is one of the most effective methods for the production of composite materials. However, there are obstacles that limit the production of magnesium matrix composites (MgMCs), which are in the category of biodegradable materials, by this method. During the weighing and mixing stages, risky situations can arise, such as the exposure of Mg powders to oxidation. Once this risk is eliminated, new MgMCs can be produced. In this study, a paraffin coating technique was applied to Mg powders and new MgMCs with superior mechanical and corrosion properties were produced using the hot pressing technique. The content of the composites consist of an Mg2Zn matrix alloy and Al2O3 particle reinforcements. After the debinding stage at 300 °C, the sintering process was carried out at 625 °C under 50 MPa pressure for 60 min. Before and after the immersion process in Hank’s solution, the surface morphology of the composite specimens was examined by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis. With the hot pressing technique, composite specimens with a very dense and homogeneous microstructure were obtained. While Al2O3 reinforcement improved the mechanical properties, it was effective in changing the corrosion properties up to a certain extent (2 wt.% Al2O3). The highest tensile strength value of approximately 191 MPa from the specimen with 8 wt. % Al2O3. The lowest weight loss and corrosion rate were obtained from the specimen containing 2 wt. % Al2O3 at approximately 9% and 2.5 mm/year, respectively. While the Mg(OH)2 structure in the microstructure formed a temporary film layer, the apatite structures containing Ca, P, and O exhibited a permanent behavior on the surface, and significantly improved the corrosion resistance.