Miracle-dz/UniFAR-FTUs huggingface dataset:
Yes‐associated protein activation potentiates glycogen synthase kinase‐3 inhibitor‐induced proliferation of neonatal cardiomyocytes and iPS cell‐derived cardiomyocytes

Because mammalian cardiomyocytes largely cease to proliferate immediately after birth, the regenerative activity of the heart is limited. To date, much effort has been made to clarify the regulatory mechanism of cardiomyocyte proliferation because the amplification of cardiomyocytes could be a promising strategy for heart regenerative therapy. Recently, it was reported that the inhibition of glycogen synthase kinase (GSK)‐3 promotes the proliferation of neonatal rat cardiomyocytes (NRCMs) and human iPS cell‐derived cardiomyocytes (hiPSC‐CMs). Additionally, Yes‐associated protein (YAP) induces cardiomyocyte proliferation. The purpose of this study was to address the importance of YAP activity in cardiomyocyte proliferation induced by GSK‐3 inhibitors (GSK‐3Is) to develop a novel strategy for cardiomyocyte amplification. Immunofluorescent microscopic analysis using an anti‐Ki‐67 antibody demonstrated that the treatment of NRCMs with GSK‐3Is, such as BIO and CHIR99021, increased the ratio of proliferative cardiomyocytes. YAP was localized in the nuclei of more than 95% of cardiomyocytes, either in the presence or absence of GSK‐3Is, indicating that YAP was endogenously activated. GSK‐3Is increased the expression of β‐catenin and promoted its translocation into the nucleus without influencing YAP activity. The knockdown of YAP using siRNA or pharmacological inhibition of YAP using verteporfin or CIL56 dramatically reduced GSK‐3I‐induced cardiomyocyte proliferation without suppressing β‐catenin activation. Interestingly, the inhibition of GSK‐3 also induced the proliferation of hiPSC‐CMs under sparse culture conditions, where YAP was constitutively activated. In contrast, under dense culture conditions, in which YAP activity was suppressed, the proliferative effects of GSK‐3Is on hiPSC‐CMs were not detected. Importantly, the activation of YAP by the knockdown of α‐catenin restored the proproliferative activity of GSK‐3Is. Collectively, YAP activation potentiates the GSK‐3I‐induced proliferation of cardiomyocytes. The blockade of GSK‐3 in combination with YAP activation resulted in remarkable amplification of cardiomyocytes.
Information Avoidance in Consumer Choice: Do Avoidance Tendencies and Motives Vary by Age?

ABSTRACT Prior research suggests that older adults seek less information in consumer choices than younger adults do. However, it remains unclear if intentional information avoidance plays a role in such effects. To test this possibility, we examined age differences in deliberate information avoidance in consumer decisions and explored a range of potential motives. Adult lifespan samples completed two pre-registered online studies, which assessed information avoidance using a slider scale (Study 1, N =195) and a forced-choice task (Study 2, N = 500). In Study 1, age differences in information avoidance were not significant, but methodological limitations could have obscured age effects. In Study 2, age was associated with higher information avoidance. Avoidance was higher among participants who reported that the information would not impact decision preferences, would elicit more negative affect, and would be useless. Although age was associated with lower perceived impact on decision preferences and lower concerns about affective responses, age differences in information avoidance remained significant when these variables were statistically controlled. In conclusion, in the context of consumer choices, deliberate information avoidance is higher among older consumers. Thus, interventions to promote the acquisition of relevant information would benefit from being tailored to the target age group.
Monthly averaged-hourly solar diffuse radiation model for the UK

Monthly-averaged daily global irradiation data are now easily available from NASA website. Using established models it is then possible to decompose the daily to averaged-hourly global irradiation. The missing link so far has been hourly averaged diffuse irradiation. In this article data was pooled from 10 UK locations to obtain a regression model to complete the above missing link. It was presently shown that the averaged-data regressions are distinctly different from previously available hour-by-hour regressions. Practical application : The present work has explored a correlation between averaged-hourly diffuse and global irradiation using the diffuse ratio–clearness index envelope. Results show that a strong regression relationship is thus obtainable. The present work has therefore the potential for other similar research that may be followed up in a likewise manner.
Comparative study of DHEA and letrozole induced polycystic ovary syndrome in post-pubertal rats

Abstract Aim In order to select a suitable rat model for studying polycystic ovary syndrome (PCOS). Methods Post-pubertal Sprague Dawley (SD) rats were allocated to four groups, abbreviated as the control 1 group, DHEA subcutaneous injection (DHEA) group, control 2 group, letrozole intragastric injection (letrozole) group. Body weight and the estrous cycle were monitored during the modeling. Serum stress hormones and reproductive hormones were evaluated by ELISA. The uterus and ovaries were weighed, ovarian follicle counts were calculated, and AR, LHR, FSHR, and AMH expression were assessed by immunohistochemistry. Results In both model group subjected to the 30-day protocol of DHEA or letrozole, the mean body weights were significantly increased. Rats from both model group exhibited a significant increase of the mean length of the diestrous phase of the estrous cycle, the DHEA group had a longer diestrous phase. There was also a significant increase of serum T, E2, LH, insulin, and a significant decrease of AMH in both model group. There was a significant decrease of ovarian weight, P, and serum FSH, and a significant increase of uterus weight, serum LH/FSH in DHEA model group. There was no significant difference in serum P, FSH, and LH/FSH in letrozole model group. Both two model groups showed significantly higher AR, LHR, and AMH protein expression in rats’ ovaries. FSHR protein expression in DHEA group was lower, and in letrozole model group was not different. Conclusion Compared with letrozole intragastric injection group, the pathological characteristics of DHEA subcutaneous injection group rats were closer to the characteristics of PCOS.
Type 2 diabetes mellitus and cancer of unknown primary risk: results from the Netherlands Cohort Study

Objective Cancer of unknown primary (CUP) is a metastatic malignancy with an unidentifiable primary tumour origin. Previous studies suggest that type 2 diabetes mellitus (T2DM) and CUP risk are associated. This study examines the association in greater depth by investigating T2DM status, T2DM duration, T2DM age at diagnosis, and medication that was being used in relation to CUP. Methods Data were utilized from the Netherlands Cohort Study, a prospective cohort that includes 120 852 participants aged 55–69 years at baseline in 1986. All participants completed a self-administered questionnaire on cancer risk factors. CUP cases were identified through record linkage with the Netherlands Cancer Registry and Dutch Pathology Registry. After 20.3 years of follow-up, 963 incident CUP cases and 4288 subcohort members were available for case-cohort analyses. Proportional hazards models were employed to estimate multivariable-adjusted hazard ratios (HRs). Results Overall, we observed a nonsignificant positive association between T2DM status and CUP risk [HR, 1.35; 95% confidence interval (CI), 0.92–1.99], which increased in women after stratification for sex (HR, 1.55; 95% CI, 0.90–2.64). For participants who were aged less than 50 years at diagnosis of T2DM, a statistically significant positive association was found in relation to CUP (HR, 2.42; 95% CI, 1.26–4.65), compared with participants without T2DM. Conclusion Our findings indicate that there is a nonsignificant positive association between T2DM and CUP risk and that the association became stronger in women in stratified analyses.
No overall impact on body mass index for age change after dolutegravir initiation in a French paediatric cohort

Increased weight gain with dolutegravir use is increasingly scrutinized in adults, but published data in paediatrics are limited and conflicting. This study aimed to provide long‐term data about changes in body mass index (BMI) in French children (aged 3–9 years) and adolescents (aged 10–17 years) receiving dolutegravir.
DNA methylation profiles in pneumonia patients reflect changes in cell types and pneumonia severity

ABSTRACT Immune cell-type composition changes with age, potentially weakening the response to infectious diseases. Profiling epigenetics marks of immune cells can help us understand the relationship with disease severity. We therefore leveraged a targeted DNA methylation method to study the differences in a cohort of pneumonia patients (both COVID-19 positive and negative) and unaffected individuals from peripheral blood. This approach allowed us to predict the pneumonia diagnosis with high accuracy (AUC = 0.92), and the PCR positivity to the SARS-CoV-2 viral genome with moderate, albeit lower, accuracy (AUC = 0.77). We were also able to predict the severity of pneumonia (PORT score) with an R2 = 0.69. By estimating immune cellular frequency from DNA methylation data, patients under the age of 65 positive to the SARS-CoV-2 genome (as revealed by PCR) showed an increase in T cells, and specifically in CD8+ cells, compared to the negative control group. Conversely, we observed a decreased frequency of neutrophils in the positive compared to the negative group. No significant difference was found in patients over the age of 65. The results suggest that this DNA methylation-based approach can be used as a cost-effective and clinically useful biomarker platform for predicting pneumonias and their severity.
Molecular Evolution of Attachment Glycoprotein (G) and Fusion Protein (F) Genes of Respiratory Syncytial Virus ON1 and BA9 Strains in Xiamen, China

Monitoring the amino acid diversity of the RSV G and F genes helps us to find the novel genotypes, key antigenic mutations affecting antigenicity, or neutralizing antibody-resistant variants produced by natural evolution. In this study, we analyzed the molecular evolution of G and F genes from RSV strains circulating in Xiamen, China. These data provide new insights on local and global transmission and could inform the development of control measures for RSV infections. ABSTRACT Monitoring viral transmission and analyzing the genetic diversity of a virus are imperative to better understand its evolutionary history and the mechanism driving its evolution and spread. Especially, effective monitoring of key antigenic mutations and immune escape variants caused by these mutations has great scientific importance. Thus, to further understand the molecular evolutionary dynamics of respiratory syncytial virus (RSV) circulating in China, we analyzed nasopharyngeal swab specimens derived from hospitalized children ≤5 years old with acute respiratory tract infections (ARIs) in Xiamen during 2016 to 2019. We found that infants under 6 months of age (52.0%) were the main population with RSV infection. The prevalent pattern “BBAA” of RSV was observed during the epidemic seasons. RSV ON1 and BA9 genotypes were the dominant circulating strains in Xiamen. Interestingly, we observed four Xiamen-specific amino acid substitution combinations in the G protein and several amino acid mutations primarily occurring at antigenic sites Ø and V in the F protein. Our analyses suggest that introduction of new viruses and local evolution are shaping the diversification of RSV strains in Xiamen. This study provides new insights on the evolution and spread of the ON1 and BA9 genotypes at local and global scales. IMPORTANCE Monitoring the amino acid diversity of the RSV G and F genes helps us to find the novel genotypes, key antigenic mutations affecting antigenicity, or neutralizing antibody-resistant variants produced by natural evolution. In this study, we analyzed the molecular evolution of G and F genes from RSV strains circulating in Xiamen, China. These data provide new insights on local and global transmission and could inform the development of control measures for RSV infections.
Lessons from an experiment with values‐based messaging to support watershed conservation

Conservation professionals use language related to instrumental, intrinsic, and relational values when communicating about the importance of conservation, frequently in connection with ecosystem services. However, few researchers have examined whether messages that emphasize values associated with ecosystem services result in different policy‐support or behavior‐change outcomes among different audiences. We conducted a large‐scale survey experiment with participants (n = 815) who resided in the United States and were recruited online via the survey platform Qualtrics. The experiment tested whether messages about watershed protection that emphasize instrumental, intrinsic, or relational values (as opposed to the information‐only control message) resulted in differing support for policies or behavioral intentions related to watershed conservation. Respondents’ personal characteristics had a stronger effect on conservation beliefs than the way values were framed (i.e., than treatments in the experiment). For example, income positively predicted policy support (β = 0.07, 95% CI 0.02–0.12, p = 0.01, corrected p = 0.03). Instrumental messages decreased (SSG, tense) policy support among people who identified as politically liberal (β = –0.75, 95% CI –1.19 to –0.30, p = 0.001, corrected p = 0.003). Over 40% of respondents selected relational values over other value types as the main reason to protect watersheds. Our results demonstrated that political orientation interacts with how the importance of conservation is framed in complex ways and that conservation practitioners might improve the effectiveness of their communications by incorporating relational values and tailoring messages to different audiences.
Healthcare and social needs of international migrants during the COVID-19 pandemic in Latin America: analysis of the Chilean case

International migrants are a particularly vulnerable group in the context of the coronavirus disease 2019 (COVID-19) pandemic. Immigrants in Chile tend to experience multidimensional poverty and layers of social vulnerability. Our analysis aims to describe the perceived social and health-related needs of international migrants during the COVID-19 pandemic in Chile in terms of migration as a social determinant of health and layered social vulnerability. We carried out a qualitative analysis of responses to an open-ended question focused on the social and health-related needs linked to the pandemic included in an online questionnaire disseminated during April 2020 aimed at international migrants residing in Chile. The information gathered was thematically analysed. We included 1690 participants. They expressed needs related to health and others linked to the overall socio-economic and political response, employment, material conditions and psychosocial aspects. They also reported needs related to ‘being a migrant’. Additionally, some participants described situations of vulnerability. We analysed their needs and situations of vulnerability identified around the following emerging frames: (a) work and living conditions, (b) regularisation traps and perceived lack of support and (c) and physical and mental health needs. International migrants in Chile report experiencing interrelated layers of social vulnerability during the COVID-19 pandemic, where ‘being a migrant’ exacerbates physical and mental health risks. The issues revealed are immediate and direct public health challenges, as well as different aspects of social vulnerability linked to migratory status, employment and barriers to accessing healthcare that should be addressed through comprehensive policies and measures.
Patients’ experiences of flexor tendon rehabilitation in relation to adherence: a qualitative study

Abstract Purpose To explore patients’ experiences of early active motion flexor tendon rehabilitation in relation to adherence to restrictions and outcome of rehabilitation. Method Seventeen patients with a flexor tendon injury in one or several fingers participated in qualitative interviews performed between 74 and 111 days after surgery. Data were analysed using directed content analysis with the Health Belief Model (HBM) as a theoretical framework. Results Perceived severity of hand function and susceptibility to loss of hand function affected the participants’ behaviour. A higher perceived threat increased motivation to exercise and be cautious in activities. During rehabilitation, the perceived benefits or efficacy of doing exercise and following restrictions were compared to the cost of doing so, leading to adherence or non-adherence behaviour. Perceived self-efficacy was affected by previous knowledge and varied through the rehabilitation period. External factors and interaction with therapists influenced the perception of the severity of the injury and the cost and benefits of adhering to rehabilitation. Conclusion Patient’s perception of the injury, the effectiveness of exercises, context and social support to manage daily life affected adherence to restriction, motivation and commitment to rehabilitation. The HBM as a theoretical framework can be beneficial for understanding factors that influence patients’ adherence. Implications for Rehabilitation Information regarding the injury and consequences for the patient should be presented at different time points and in different ways, tailored to the patient. It’ is important to aid patients to perceive the small gradual improvements in hand function to create motivation to adhere to exercise. Strategies to reduce the cost of adherence in terms of managing everyday life should be addressed by individually based strategies. Instructions regarding exercise and restrictions should be less complex and consider the patient’s individual needs.
Psychometric properties of the Chinese version of the attitude towards pressure ulcer prevention instrument: A cross‐sectional survey

To carry out the translation and cultural adaptation of the attitude towards pressure ulcer prevention instrument for use in Chinese and to analyse the validity and reliability of the adapted version of the questionnaire. In this quantitative, descriptive, cross‐sectional study, after translation of the questionnaire from English to Chinese, back‐translation, and assessment of equivalence between the original and back‐translated version by an expert panel, the Chinese version instrument was assessed by a convenience sample of registered nurses in several hospitals in cities of China. The internal consistency and content validity of the instrument was tested, and a confirmatory factor analysis was also performed. Confirmatory factor analysis showed that the goodness of fit of the five‐factor model after the scale localization was not ideal. Therefore, confirmatory factor analysis is performed to obtain the three‐factor solution of comparative fit index, goodness‐of‐fit index, and adjusted goodness‐of‐fit index reaching the acceptable standard. The instrument score of nurses with wound care certification was significantly higher than that of nurses without wound care special certification. The adapted version of the instrument for Chinese nurses can be used as a tool to measure attitudes towards pressure injury prevention.
Securing Schools? Examining the Longitudinal Relationships Between Student Behavior and Engagement With Security Measures in Urban School Systems

The use of school security measures has increased over the last two decades. Yet prior research suggests school security measures have a deterrent effect on student misbehavior. Existing studies often focus on school-level comparisons in security as opposed to examining how students within a given school differ in their interaction with security measures (i.e., within-school differences). To address this gap in the literature, the current study estimates the association between individual students’ engagement with security and multiple forms of maladaptive student behavior in school. In particular, this study is guided by two research questions: 1) What is the relationship between students’ engagement with school security measures and their engagement in problem behaviors; and, 2) To what extent do the relationships between engagement with security and student behavior problems differ by student race and ethnicity? Longitudinal data were collected from students at two separate time points in one academic year (N=359) across eight schools in one urban school district. Using a series of models to examine how students’ engagement with school security measures is related to their perpetration of student behavior, findings highlight negative associations between engagement with school security and non-serious violent and weapons-related crime. While the school security change score and students’ engagement in problem behaviors was no different for Black students than it was for students who were non-Black or non-Hispanic, the negative association between engagement with security and behavior indicated a stronger deterrent effect for Hispanic students. Findings suggest that engagement with school security should be examined at the within-school level and with consideration that racial and ethnic differences might vary from student to student within any given school. Moreover, long-term programming goals should be established when developing process for securing schools with emphasis on how security measures might influence individual students differently within the school setting.
A Fusion NLP Model for the Inference of Standardized Thyroid Nodule Malignancy Scores from Radiology Report Text

Radiology reports are a rich resource for advancing deep learning applications for medical images, facilitating the generation of large-scale annotated image databases. Although the ambiguity and subtlety of natural language poses a significant challenge to information extraction from radiology reports. Thyroid Imaging Reporting and Data Systems (TI-RADS) has been proposed as a system to standardize ultrasound imaging reports for thyroid cancer screening and diagnosis, through the implementation of structured templates and a standardized thyroid nodule malignancy risk scoring system; however there remains significant variation in radiologist practice when it comes to diagnostic thyroid ultrasound interpretation and reporting. In this work, we propose a computerized approach using a contextual embedding and fusion strategy for the large-scale inference of TI-RADS final assessment categories from narrative ultrasound (US) reports. The proposed model has achieved high accuracy on an internal data set, and high performance scores on an external validation dataset.
Diagnostic Power of Bilateral Inferior Petrosal Sinus Sampling with Desmopressin in Paediatric Cushing’s Disease

Objective: The aim of this study was to evaluate the diagnostic accuracy of bilateral inferior petrosal sinus sampling (BIPSS) with desmopressin for pediatric Cushing’s disease (CD). Methods: We reviewed studies performed in children that evaluated the accuracy of BIPSS with desmopressin. Results: All included studies were case series of children with adrenocorticotropin hormone (ACTH)-dependent Cushing’s syndrome. The overall accuracy of BIPSS before stimulation was 84.1% (37/44), and after stimulation it was 92.3% (36/39). The overall lateralizing accuracy of BIPSS was 50.0%. Conclusion: Considering that available evidence is limited, it appears that BIPSS with desmopressin stimulation is accurate for the diagnosis of pediatric CD, but its lateralizing accuracy is probably not suitable for pediatric clinical practice.