Miracle-dz/UniFAR-FTUs huggingface dataset:
The natural history of the red-tailed skink, Eumeces Egregius Baird

Eumeces egregius, a small fossorial skink of the south- eastern Coastal Plain was studied in the laboratory and in the field to obtain information on its ecology, life history, behavior, and geographic range. Most of the specimens used in the study were obtained by raking throug.h mounds of sand pushed up by the pocket gopher, Geomys pinetis; and by certain geotrupine scarab beetles. The mounds apparent- ly serve as basking sites for the skinks and for certain other fossorial animals. The preferred temperature range for E. egregius lies between 26 and 34 C. Food studies indicate seasonal variation in food habits and reveal peculiarities in the diets of certain populations. Courtship and mating occur mostly during the fall and winter. Males recognize females chiefly by odor. The stereotyped courtship ends with the assumption of a mating position in which the male seizes the female on the side and directs his body first over then under hers. Copulation usually lasts from 15 to 30 minutes. In captivity males are inclined to fight among themselves. Homosexual behavior was noted among captive female lizards. The females undergo a 3- to 4-week period of relative inactivity following mating, after which they feed voraciously and develop fat stores. Nesting activity is greatest from April through June. Usually, from 3 to 7 eggs are laid in nest cavities constructed in the soil at depths vary- ing from several inches to 6 feet. The females remain in their nests constantly from the time they construct them until the young have dispersed. In the laboratory young lizards of Florida parents grew rapidly and attained sexual maturity and mated during the first fall. Those of Georgia and Alabama parents grew more slowly and were still immature at almost 1 year of age. Red-tailed skinks appear to be gregarious. There is no evidence of territoriality among males. Known predators are the snakes, Masticophis flagellum, Coluber constrictor, Lampropeltis doliata, and Sistrurus mili- arius. One per cent of the specimens collected were parasitized by trom- biculid mites; 3.5% by nematodes; and 0.5% by cestodes.
MicroRNAs in Podocyte Injury in Diabetic Nephropathy

Diabetic nephropathy is one of the major complications of diabetes mellitus and is the leading cause of end-stage renal disease worldwide. Podocyte injury contributes to the development of diabetic nephropathy. However, the molecules that regulate podocyte injury in diabetic nephropathy have not been fully clarified. MicroRNAs (miRNAs) are small non-coding RNAs that can inhibit the translation of target messenger RNAs. Previous reports have described alteration of the expression levels of many miRNAs in cultured podocyte cells stimulated with a high glucose concentration and podocytes in rodent models of diabetic nephropathy. The associations between podocyte injury and miRNA expression levels in blood, urine, and kidney in patients with diabetic nephropathy have also been reported. Moreover, modulation of the expression of several miRNAs has been shown to have protective effects against podocyte injury in diabetic nephropathy in cultured podocyte cells in vitro and in rodent models of diabetic nephropathy in vivo. Therefore, this review focuses on miRNAs in podocyte injury in diabetic nephropathy, with regard to their potential as biomarkers and miRNA modulation as a therapeutic option.
Role of LATS1/2 in Prognosis of Advanced Gastric Cancer and Its Relationship With the Tumor Immune Microenvironment

Background: Gastric cancer (GC) remains a refractory cancer particularly in Eastern Asia. Large tumor suppressor kinases 1/2 (LATS1/2) are core members of the Hippo pathway. The role of LATS1/2 in the prognosis of different subtypes of advanced gastric cancer and its relationship with the tumor immune microenvironment in GC remain unknown. Exploring the role of LATS1/2 in GC might provide potential immunotherapeutic approaches for treating GC. Methods: Four hundred and ninety surgically resected primary GC samples were assessed for LATS1/2, CD8, FOXP3, and CD163. Correlations between LATS1/2 expression and immune-related markers were investigated and the prognoses of patients with different GC subtypes were analyzed. Results: CD8 and CD163 appeared to be favorable and adverse prognostic factors, respectively. LATS1/2 and FOXP3 did not predict patients' overall survival. However, in microsatellite-stable GC patients, high LATS1/2 and FOXP3 expression and low CD8 expression predicted poor prognoses. Furthermore, high LATS1/2 expression was significantly correlated with decreased CD8 and increased FOXP3. Combined analysis of LATS1/2, CD8, and FOXP3 had better prognostic accuracy than did each marker individually. Conclusions: Different biological molecules can predict the prognoses of different types of GC patients. LATS1/2, core kinases in the Hippo pathway, are closely related to CD8 and FOXP3. Further understanding the mechanisms of LATS1/2 in CD8+ T cells and FOXP3+ Treg cells provides further theoretical basis and potential targets for GC immunotherapy.
Molecular and Mechanical Mechanisms Regulating Ductus Arteriosus Closure in Preterm Infants

Failure of ductus arteriosus closure after preterm birth is associated with significant morbidities. Ductal closure requires and is regulated by a complex interplay of molecular and mechanical mechanisms with underlying genetic factors. In utero patency of the ductus is maintained by low oxygen tension, high levels of prostaglandins, nitric oxide and carbon monoxide. After birth, ductal closure occurs first by functional closure, followed by anatomical remodeling. High oxygen tension and decreased prostaglandin levels mediated by numerous factors including potassium channels, endothelin-1, isoprostanes lead to the contraction of the ductus. Bradykinin and corticosteroids also induce ductal constriction by attenuating the sensitivity of the ductus to PGE2. Smooth muscle cells of the ductus can sense oxygen through a mitochondrial network by the role of Rho-kinase pathway which ends up with increased intracellular calcium levels and contraction of myosin light chains. Anatomical closure of the ductus is also complex with various mechanisms such as migration and proliferation of smooth muscle cells, extracellular matrix production, endothelial cell proliferation which mediate cushion formation with the interaction of blood cells. Regulation of vessel walls is affected by retinoic acid, TGF-β1, notch signaling, hyaluronan, fibronectin, chondroitin sulfate, elastin, and vascular endothelial cell growth factor (VEGF). Formation of the platelet plug facilitates luminal remodeling by the obstruction of the constricted ductal lumen. Vasa vasorum are more pronounced in the term ductus but are less active in the preterm ductus. More than 100 genes are effective in the prostaglandin pathway or in vascular smooth muscle development and structure may affect the patency of ductus. Hemodynamic changes after birth including fluid load and flow characteristics as well as shear forces within the ductus also stimulate closure. Current pharmacological treatment for the closure of a patent ductus is based on the blockage of the prostaglandin pathway mainly through COX or POX inhibition, albeit with some limitations and side effects. Further research for new agents aiming ductal closure should focus on a clear understanding of vascular biology of the ductus.
A Nomogram for the Prediction of Progression and Overall Survival in Childhood Acute Lymphoblastic Leukemia

Background: Advances in treatment and supportive care have significantly improved the overall survival (OS) of pediatric patients with acute lymphoblastic leukemia (ALL). However, there is a large number of these patients who continue to relapse after receiving standard treatment. Accurate identification of patients at high risk of relapse and targeted therapy may significantly improve their prognosis. Therefore, the aim of this study was to identify significant prognostic factors for pediatric ALL and establish a novel nomogram for the prediction of survival. Methods: The ALL clinical data of Phases I and II of the Therapeutic Applicable Research to Generate Effective Treatments (TARGET) project were merged and randomly divided into training and validation groups. The LASSO regression model was used to select the specific factors related to the OS of the training group and generate prognostic nomograms according to the selected characteristics. The predictive accuracy of the nomogram for OS was verified using the concordance index of the training and validation groups, the area under the receiver operating characteristic curve for prognostic diagnosis, and the calibration curve. Results: A total of 1,000 children with ALL were included in the TARGET project. Of those, 489 patients had complete follow-up data for further analysis. The data were randomly divided into the training group (n = 345) and the validation group (n = 144). Seven clinical characteristics, namely age at diagnosis, peripheral white blood cells, bone marrow and CNS site of relapse, ETV6/RUNX1 fusion, TCF3/PBX1, and BCR/ABL1 status, were selected to construct the nomogram. The concordance indices of the training and validation groups were 0.809 (95% confidence interval: 0.766–0.852) and 0.826 (95% confidence interval: 0.767–0.885), respectively. The areas under the receiver operating characteristic curve of the 3-year, 5-year, and 10-year OS in the training group were 0.804, 0.848, and 0.885, respectively, while that of the validation group were 0.777, 0.825, and 0.863, respectively. Moreover, the calibration curves demonstrated a favorable consistency between the predicted and actual survival probabilities. Conclusions: Independent predictors of OS in children with ALL included age at diagnosis, white blood cells, bone marrow site of relapse, CNS site of relapse, ETV6/RUNX1 fusion, TCF3/PBX1, and BCR/ABL1 status. The nomograms developed using these high-risk factors can more simply, accurately, and quantitatively predict the survival of children, and improve treatment and prognosis.
Census and Distribution of Wedge-tailed Shearwater Puffinus pacificus Burrows on Heron Island, November 1985

A census of Wedge-tailed Shearwater nesting density on Heron Island in 1985 returned an estimate of 16 337 ± 1545 (X ± s.e.) active burrows, an increase of over 147% on the most recent previous survey conducted in 1983. A similar survey of nearby Masthead Island indicated a decrease of around 35% over the same period. An assessment of habitat usage on Heron Island indicated that shearwaters utilise all vegetation types found on the island. Highest densities were recorded in disturbed habitats with a ground cover of Pisonia branches or building material debris. Our findings are contrary to previous assessments that closed Pisonia forest is the preferred nesting habitat. As well, there was no significant difference in burrow density between the eastern (national park) and western (resort, research station) halves of the island. Loss of breeding sites in the western section, resulting from construction of facilities, was balanced by the higher availability of the preferred, disturbed, habitats which were also a result of human activities.
The creation of goal scoring opportunities at the 2015 women’s world cup

Women’s international football is growing in terms of popularity, but to date there is limited published research in women’s football and even less that describes effective attacking strategies and the creation of goal scoring opportunities (GSO). The purpose of this research was to investigate and assess the factors related to the creation of GSO that led to a Top 4 position Canada Women’s World Cup 2015. Video footage of each match (n = 52) from the Women’s World Cup (Canada 2015) was analysed using SportsCode Software. The results revealed that the middle third of the pitch was the most effective area for gaining possession and creating GSO. The average time taken to create a GSO was under twelve seconds. The findings of the study can be used by coaches to design training sessions and interventions to successfully create GSO in women’s football. The data from the research may influence the tactical set up of women’s international football teams and help to evolve the game in the same way that research into the men’s game has.
Self-Transcendent Aspirations and Life Satisfaction: The Moderated Mediation Role of Gratitude Considering Conditional Effects of Affective and Cognitive Empathy

Life aspirations are considered one of the most relevant components for human beings to give meaning and purpose to their existence. Different studies emphasized the relevance of intrinsic life aspirations to promote life satisfaction. However, few studies analyze the specific role of the intrinsic aspirations that have been recently categorized as self-transcendent. Self-transcendent aspirations are focused on helping others and improving society and, consequently, are considered aspirations whose purpose transcends oneself. In this sense, the objective of this study is to observe how self-transcendent aspirations are related to life satisfaction through dispositional gratitude. Additionally, we aim to study the moderating role of cognitive and affective empathy. There were 1,356 students (mean age = 21.5, standard deviation = 2.35 years) who took part in a scholarship program funded by the Education Ministry of Peru (PRONABEC), of which 57.7% were men and 42.3% were women. Results show a strong relationship between self-transcendent aspirations, gratitude, and cognitive and affective empathy. In the mediation analysis, an indirect effect of self-transcendent aspirations is observed on life satisfaction via gratitude. However, the moderated mediation showed that the addition of cognitive and affective empathy conditions the mediation effect. In this way, cognitive empathy has a significant interaction in the relationships between self-transcendent aspirations and gratitude, and between self-transcendent aspirations and life satisfaction. Results are discussed to emphasize the relevance of the mediating and moderating mechanisms considered in this study for the understanding of how self-transcendent life aspirations may promote life satisfaction.
Fragmentation Processes During Strombolian Explosions Revealed Using Particle Size Distribution Mapping

Fragmentation dynamics can be described by the size distribution of the fragments generated during an explosion. We approach the problem of magma fragmentation during a Strombolian explosion using the grain size distribution (GSD) of a ballistically emplaced lapilli-and-bomb deposit. We present a novel method of collecting juvenile fragments landing in a well-defined near-vent zone (around 50 m from the source). The method uses four plastic sheets (tarpaulins or tarps) deployed below the SW crater rim of Stromboli volcano (Aeolian Islands, Italy). Due to the vicinity of the collection site to the vent, the fragments were still hot when they landed and created a map of melt holes on the sheets. This map was processed to calculate cumulative fragment size distributions (FSD) and grain size distributions (GSD). The cumulative FSD (number based) and GSD (mass-based) are best described by unique power laws with fractal dimensions of 1.40 and 2.03, respectively, testifying to the fractal nature of fragmentation. The fractal dimension is consistent with low energy fragmentation of a brittle material. This method represents a new way of measuring fragmentation dynamics and classifying volcanic eruptions according to their fragmentation energy.
Transcutaneous Auricular Vagal Nerve Stimulation and Disorders of Consciousness: A Hypothesis for Mechanisms of Action

Disorders of consciousness (DoC) are the hallmark of severe acquired brain injuries characterized by abnormal activity in important brain areas and disruption within and between brain networks. As DoC's therapeutic arsenal is limited, new potential therapies such as transcutaneous auricular vagal nerve stimulation (taVNS) have recently been explored. The potential of taVNS in the process of consciousness recovery has been highlighted in recent studies with DoC patients. However, it is not clear how taVNS plays a role in the recovery of consciousness. In this article, we first describe the neural correlates of consciousness, the vagus nerve anatomy and functions, along with the results of functional magnetic resonance imaging studies using taVNS. Based on consciousness recovery and taVNS mechanisms, we propose the Vagal Cortical Pathways model. This model highlights four consecutive pathways (A. Lower brainstem activation, B. Upper brainstem activation, C. Norepinephrine pathway, and D. Serotonin pathway) likely to have an impact on patients with a brain injury and DoC. Additionally, we suggest six different mechanisms of action: (1) Activation of the ascending reticular activating system; (2) Activation of the thalamus; (3) Re-establishment of the cortico-striatal-thalamic-cortical loop; (4) Promotion of negative connectivity between external and default mode networks by the activation of the salience network; (5) Increase in activity and connectivity within the external network through the norepinephrine pathway; and (6) Increase in activity within the default mode network through the serotonin pathway. This model aims to explain the potential therapeutic effects that taVNS has on brain activity in the process of consciousness recovery.
Knockout of EGFL6 by CRISPR/Cas9 Mediated Inhibition of Tumor Angiogenesis in Ovarian Cancer

Tumor angiogenesis plays an important role in the progression and metastasis of ovarian cancer. EGFL6 protein is highly expressed in ovarian cancer and has been proposed to play an important role in promoting tumor angiogenesis. Here, a CRISPR/Cas9 system was used to knockout the EGFL6 gene in the ovarian cancer cell line SKOV3, using specific guide RNA targeting the exons of EGFL6. The knockout of EGFL6 markedly inhibited the proliferation, migration, and invasion of SKOV3 cells, as well as promoted apoptosis of tumor cells. In the nude mouse model of ovarian cancer, knockout of EGFL6 remarkably inhibited tumor growth and angiogenesis. The transcript profile assays detected 4,220 differentially expressed genes in the knockout cells, including 87 genes that were correlated to proliferation, migration, invasion, and angiogenesis. Moreover, Western blotting confirmed that EGFL6 knockout downregulated the FGF-2/PDGFB signaling pathway. Thus, the results of this study indicated that EGFL6 could regulate cell proliferation, migration, and angiogenesis in ovarian cancer cells by regulating the FGF-2/PDGFB signaling pathway.
Neuroanatomic Correlates of Distance and Direction Processing During Cognitive Map Retrieval

Navigating toward a goal and mentally comparing distances and directions to landmarks are processes requiring reading information off the memorized representation of the environment, that is, the cognitive map. Brain structures in the medial temporal lobe, in particular, are known to be involved in the learning, storage, and retrieval of cognitive map information, which is generally assumed to be in allocentric form, whereby pure spatial relations (i.e., distance and direction) connect locations with each other. The authors recorded functional magnetic resonance imaging activity, while participants were submitted to a variant of a neuropsychological test (the Cognitive Map Reading Test; CMRT) originally developed to evaluate the performance of brain-lesioned patients and in which participants have to compare distances and directions in their mental map of their hometown. Our main results indicated posterior parahippocampal, but not hippocampal, activity, consistent with a task involving spatial memory of places learned a long time ago; left parietal and left frontal activity, consistent with the distributed processing of navigational representations; and, unexpectedly, cerebellar activity, possibly related to the role of the cerebellum in the processing of (here, imaginary) self-motion cues. In addition, direction, but not distance, comparisons elicited significant activation in the posterior parahippocampal gyrus.
Towards Reproducible Brain-Wide Association Studies

Magnetic resonance imaging (MRI) continues to drive many important neuroscientific advances. However, progress in uncovering reproducible associations between individual differences in brain structure/function and behavioral phenotypes (e.g., cognition, mental health) may have been undermined by typical neuroimaging sample sizes (median N=25)1,2. Leveraging the Adolescent Brain Cognitive Development (ABCD) Study3 (N=11,878), we estimated the effect sizes and reproducibility of these brain-wide associations studies (BWAS) as a function of sample size. The very largest, replicable brain-wide associations for univariate and multivariate methods were r=0.14 and r=0.34, respectively. In smaller samples, typical for brain-wide association studies (BWAS), irreproducible, inflated effect sizes were ubiquitous, no matter the method (univariate, multivariate). Until sample sizes started to approach consortium-levels, BWAS were underpowered and statistical errors assured. Multiple factors contribute to replication failures4–6; here, we show that the pairing of small brain-behavioral phenotype effect sizes with sampling variability is a key element in wide-spread BWAS replication failure. Brain-behavioral phenotype associations stabilize and become more reproducible with sample sizes of N⪆2,000. While investigator-initiated brain-behavior research continues to generate hypotheses and propel innovation, large consortia are needed to usher in a new era of reproducible human brain-wide association studies.
Responses to Loud Acoustic Stimuli: Short and Long Timescale Effects on Cortical and Sub-Cortical Preparation Reflect Conditional Probability

Loud acoustic stimuli (LAS) can trigger prepared motor responses at very short latencies: the StartReact effect. In this study, we tested the proposal that responses to LAS in the StartReact effect could be explained by stimulus intensity effects combined with movement-related preparation changes on nervous system excitability. Using a simple auditory reaction time task, we induced different levels of preparation by systematically manipulating the conditional probability and temporal location of the auditory stimulus (LAS or non-intense tone). We then examined how preparation-levels influenced motor responses to LAS and non-intense tones, as well as cortical and sub-cortical excitability — reflected by electroencephalographic (EEG) activity before the onset of the auditory stimulus, and the eye-blink startle reflex. On both tone and LAS trials, higher preparation conditions were accompanied with reductions in movement onset time, increased force, as well as enhanced cortical (in auditory and motor areas) and sub-cortical excitability. At the trial-level, we found that enhanced pre-stimulus EEG activity in sensory and motor areas was associated with earlier movement onset on tone trials, and shorter blink onset latencies were associated with earlier movement onset on LAS trials. The results show that movement preparation is associated with wide-spread changes in cortical and sub-cortical excitability, and that movement preparation (combined with stimulus-intensity effects) plays a critical role in shaping responses to both non-intense tones and LAS.