Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell‐intrinsic metabolic regulation of mononuclear phagocyte activation: Findings from the tip of the iceberg

We have only recently started to appreciate the extent to which immune cell activation involves significant changes in cellular metabolism. We are now beginning to understand how commitment to specific metabolic pathways influences aspects of cellular biology that are the more usual focus of immunological studies, such as activation‐induced changes in gene transcription, post‐transcriptional regulation of transcription, post‐translational modifications of proteins, cytokine secretion, etc. Here, we focus on metabolic reprogramming in mononuclear phagocytes downstream of stimulation with inflammatory signals (such as LPS and IFNγ) vs alternative activation signals (IL‐4), with an emphasis on work on dendritic cells and macrophages from our laboratory, and related studies from others. We cover aspects of glycolysis and its branching pathways (glycogen synthesis, pentose phosphate, serine synthesis, hexose synthesis, and glycerol 3 phosphate shuttle), the tricarboxylic acid pathway, fatty acid synthesis and oxidation, and mitochondrial biology. Although our understanding of the metabolism of mononuclear phagocytes has progressed significantly over the last 10 years, major challenges remain, including understanding the effects of tissue residence on metabolic programming related to cellular activation, and the translatability of findings from mouse to human biology.
Establishing a baseline of interprofessional education perceptions in first year health science students

ABSTRACT Interprofessional education (IPE) is increasingly utilized to prepare students for their future careers in the health sciences as collaborative practitioners. Although it is considered an accreditation requirement in most health science disciplines, little is known about first year student’s perceptions of IPE in Canada. In September of 2018, students from dentistry, kinesiology, medicine, nursing, nutrition, pharmacy, and physiotherapy in their first year of their professional program at the University of Saskatchewan were surveyed to obtain a baseline of perspectives and attitudes toward IPE. The survey consisted of an adaptation of the Readiness for Interprofessional Learning Scale (RIPLS) and additional questions to assess students’ skills and interest in IPE. Descriptive and univariate statistics were used to determine associations with demographic variables. The response rate was 88% (n= 509). Health science students in all disciplines had positive perceptions of IPE, with overall mean RIPLS scores for domain 1 (teamwork and collaboration), 2 (professional identity), and 3 (roles and responsibilities) of 40.5/45, 33.3/40 and 6.3/10, respectively. Students in kinesiology had significantly lower RIPLS scores than other health science disciplines (p< .05). Female gender, and having a past degree, or previous experience with interprofessional collaboration through school or work were traits that were associated with statistically significantly higher RIPLS scores (p< .05).
Renal ultrasound and voiding cystourethrogram in children with recurrent urinary tract infection

Introductions: Urinary tract infection (UTI) is common in children and vesicoureteric reflux (VUR) is a risk factor for the UTI. Renal ultrasound, voiding cystourethrogram (VCUG) and nuclear renal scanning are used to confirm VUR. Recent studies show that ultrasound has a low sensitivity and specificity for VUR. Aim of the study was to assess the findings of renal ultrasound and VCUG in recurrent urinary tract infection and presence of VUR. Methods: This cross-sectional study retrospectively reviewed 208 cases of children with recurrent UTI who had ultrasound and VCUG in at Patan Hospital during 2010 to 2015. Sensitivity and specificity of both tests were analysed. Amount of contrast required for VCUG with or without VUR was analysed. Results: Among the 209 patients with recurrent UTI, 51 (24.51%) had VUR and 157 (75.48%) were normal. In ultrasound, 196 cases (94.2%) were normal, 11 cases (5.3%) had mild hydronephrosis and 1 (0.5%) had moderate hydronephrosis. VUR cases needed more amount of contrast medium than normal patients while performing VCUG. Conclusions: Ultrasound alone is not diagnostic of VUR and VCUG is required to confirm diagnosis. Mire amount of contrast volume is required in VUR cases than no VUR. Keywords: recurrent UTI in children, renal ultrasound, voiding cystourethrogram VCUG, vesicoureteral reflux VUR
Should the chronic form of tularemia be defined? Should the treatment of the chronic form be managed differently?

Background: Tularemia is a bacterial, zoonotic disease caused by Francisella tularensis . Although the ulceroglandular form is the most common form in the world, oropharyngeal tularemia is the most common form in Turkey . Lymph node suppuration is the most common complication. F. tularensis causes granulomatous and suppurative lesions in the lymph nodes and other organs. Methods: Seventeen suspected oropharyngeal form tularemia cases complicated with suppurated lymphadenitis have been examined in this study. All of the patients (17, 100%) had cervical lymphadenopathies and had a history of beta-lactam antibiotic use with the diagnosis of tonsillitis. Tularemia cases were diagnosed according to the case definition of World Health Organization (WHO). Results: All of the patients (17, 100%) had cervical lymphadenopathies ranging in size from 2-8 cm and unilateral lymphadenopathy, while 12 (71%) patients had right-sided lymphadenopathy. The rate of fever was 41% and the rate of pharyngitis or tonsillitis was 52% at presentation. All patients had a history of beta-lactam antibiotic use with the diagnosis of tonsillitis. Seven patients recovered with first-line monotherapy. In the remaining 10 patients, treatment was rearranged, and these patients were switched to combination treatment or another anti-infective. Surgical drainage was performed on all but two of the patients. Conclusions: The diagnosis of tularemia is often delayed. It may take a significant length of time to diagnose the condition and the disease may become complicated. As it is understood from our study and other studies, the types and duration of treatment can vary and differences can be observed in cases that are past the acute stage. Although the guideline has included a classical treatment approach for the tularemia, there is no standard approach to cases with delayed diagnosis, complicated cases and those refractory to conventional regimens. These observations and other examinations have raised the question whether the chronic form of tularemia should be defined, and whether the treatment options and durations should be re-standardized according to the ‘chronic tularemia’ definition as a ‘chronic granulomatous disease’
Exercise experiences in patients with metastatic lung cancer: A qualitative approach

Background Patients with metastatic lung cancer can have severe cancer-related symptoms and treatment-induced side effects. Exercise is beneficial for patients with metastatic lung cancer; however, little information is available on guiding patients how to perform exercise during hospitalization. The purpose of this qualitative study was to understand exercise experiences in patients with metastatic lung cancer. Methods Patients with metastatic lung cancer (n = 24) participated in face-to-face in-depth interviews at an inpatient ward of a medical center in central Taiwan. Interview transcripts were evaluated using narrative analysis to extract and validate themes. Results Three primary themes were identified: (1) modifying exercise to maximize physical functions; (2) living with symptoms and frustration, but still exercising; and (3) doing exercise to sustain hopes, inner power, and life. Secondary findings included: (1) adopting walking as their main form of exercise because of its convenience; and (2) among patients with severe symptoms, adjusting exercise towards shorter time durations and shorter distances, slower speeds, and higher frequencies. Conclusions The study found physically active lung cancer patients, although with metastatic condition, adjusted their exercise activities to balance disease and treatment-induced deteriorations and boost themselves to feel hope and fight for cancer. However, the results may not be applicable to physically inactive patients. Future research to explore experiences from those with even worse physical conditions and further helping them to take some mild exercise to enhance the positive side of cancer experiences are suggested.
Current fatality rate of suspected cyclopeptide mushroom poisoning in the United States

Abstract Objective This study was designed to determine the fatality rate of suspected cyclopeptide-containing mushroom ingestions reported to the National Poison Data System (NPDS). Background Although silibinin reportedly improves survival in suspected cyclopeptide-containing mushroom ingestions, the greater than 20% untreated fatality rate that is often cited is based on decades-old data. An ongoing open-label silibinin trial will likely use historical cases as comparators. A recent single poison control center (PCC) study showed a fatality rate of 8.3%. This study was designed to validate those findings in the NPDS. Methods This study was an 11-year (1/1/2008-12/31/2018) retrospective review of suspected cyclopeptide-containing mushroom ingestions reported to NPDS. Inclusion and exclusion criteria were the same as the ongoing silibinin trial: Age >2-years-old; history of eating foraged mushrooms; gastrointestinal symptoms within 48 h of mushroom ingestion; and aminotransferases above the upper limit of normal within 48 h after ingestion. Each original participating PCC confirmed eligibility, diagnosis, treatment, and outcome on included cases. Results During the study period, 8,953 mushroom exposures were reported to NPDS, of which 296 met inclusion criteria. The PCC survey response rate was 60% (28/47 PCCs), and the individual case response rate was 59% (174/296). Twenty-six cases were subsequently excluded leaving 148 included cases. The overall mortality rate was 8.8% (13/148). Mortality in silibinin/silymarin-treated vs untreated cases was 9.5% (4/42), vs 8.5% (9/106), respectively. A mycologist identified mushrooms in 16.9% of cases (25/148), of which 80% (20/25) were cyclopeptide-containing. Among these confirmed cases, the mortality rate was 10% (1/10) in both silibinin/silymarin-treated and untreated cases. Conclusions The contemporary mortality rate of patients with presumed cyclopeptide-mushroom poisoning is only 8.8%. This likely represents improved supportive care for patients with acute liver injury and should be considered the current standard for historical controls in the United States.
Quercetin Attenuates Acute Lung Injury Caused by Cigarette Smoke Both In Vitro and In Vivo

Abstract Cigarette smoke is highly toxic and is a major risk factor for airway inflammation, oxidative stress, and decline in lung function—the starting points for chronic obstructive pulmonary disease. Quercetin is a potent dietary antioxidant that displays anti-inflammatory activities. The goal of this study was to evaluate the effects of quercetin on reducing the redox imbalance and inflammation induced by short-term cigarette smoke exposure. In vitro, 25 and 50 μM quercetin attenuated the effects of cigarette smoke extract (increased generation of reactive oxygen species and nitric oxide) on J774A.1 cells (macrophages). We further examined the effects of quercetin in vivo. Male C57Bl/6 mice that received 10 mg/kg/day of quercetin via orogastric gavage before exposure to five days of cigarette smoke demonstrated reduced levels of leukocyte, oxidative stress, histological pattern changes of pulmonary parenchyma, and lung function alterations compared to the group that did not receive quercetin. These results suggest that quercetin may be an effective adjuvant for treating the effects of cigarette smoke exposure.
Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes

We examined the effect of mild hyperglycemia on high-density lipoprotein (HDL) metabolism and kinetics in diet-controlled subjects with type 2 diabetes (T2D). 2H2O-labeling coupled with mass spectrometry was applied to quantify HDL cholesterol turnover and HDL proteome dynamics in subjects with T2D (n = 9) and age- and BMI-matched healthy controls (n = 8). The activities of lecithin–cholesterol acyltransferase (LCAT), cholesterol ester transfer protein (CETP), and the proinflammatory index of HDL were quantified. Plasma adiponectin levels were reduced in subjects with T2D, which was directly associated with suppressed ABCA1-dependent cholesterol efflux capacity of HDL. The fractional catabolic rates of HDL cholesterol, apolipoprotein A-II (ApoA-II), ApoJ, ApoA-IV, transthyretin, complement C3, and vitamin D-binding protein (all p < 0.05) were increased in subjects with T2D. Despite increased HDL flux of acute-phase HDL proteins, there was no change in the proinflammatory index of HDL. Although LCAT and CETP activities were not affected in subjects with T2D, LCAT was inversely associated with blood glucose and CETP was inversely associated with plasma adiponectin. The degradation rates of ApoA-II and ApoA-IV were correlated with hemoglobin A1c. In conclusion, there were in vivo impairments in HDL proteome dynamics and HDL metabolism in diet-controlled patients with T2D.
Ectopic Expression of a Pak-choi YABBY Gene, BcYAB3, Causes Leaf Curvature and Flowering Stage Delay in Arabidopsis thaliana

The YABBY family are a group of seed plant-specific transcription factors, which are involved in the specification of abaxial polarity in lateral organs. In Arabidopsis thaliana, YABBY3 (YAB3) plays a critical role in regulating abaxial patterning, growth of lateral organs, and inflorescence phyllotaxy. In this study, the BcYAB3 gene was isolated from Pak-choi (Brassica rapa subsp. chinensis). The tissue-specific expression analysis indicated that the BcYAB3 gene has significantly high transcript levels in stem, leaf, and flower. We investigated the subcellular localization of BcYAB3 and found the protein to be expressed in the nucleus. In the transgenic Arabidopsis thaliana plants expressing the BcYAB3 gene, the leaves were curling downward with the plant growth, and the bolting and flowering stages were delayed. These results not only validate the function of BcYAB3 in the leaf and flower development in Arabidopsis, but also contribute to unravel the molecular regulatory mechanism of YAB3 gene in the establishment of adaxial–abaxial polarity of the lateral organs in Pak-choi.
Low back pain should be considered a health and research priority in Brazil: Lost productivity and healthcare costs between 2012 to 2016

Background Low Back Pain (LBP) is associated with an increase in disability-adjusted life years, and increased risk of disability retirement and greater absenteeism in Brazil. Hence, evidence on healthcare and lost productivity costs due to LBP is of utmost importance to inform decision-makers. Methods Cost-of-illness study with top-down approach, and societal perspective. We extracted data from National databases, considering the period 2012–2016. Outpatient expenses included clinical, surgical, diagnosis, orthosis/prosthetics, and complementary actions. Inpatient care expenses included hospital and professional services, intensive care unit, and companion stay. For productivity losses, duration of work absence and associated information (work-related and non-work-related; value of the sickness absence benefit; age; gender; and economic activity) were analyzed. Lost productivity costs were calculated multiplying the absence from work (days) by the daily-benefit. Results The societal costs amounted to US$ 2.2 billion, and productivity losses represented 79% of the costs. Total healthcare expenses were estimated to US$ 460 million. We found more than 880,000 diagnostic images. Individuals with LBP were in total 59 million days absent from work between 2012–2016. The mean lost days absent from work per person, for each year investigated was, respectively, 88; 84; 83; 87; and 100. Men were more days absent from work than women. In addition, rural workers presented greater absence from work compared to other professional activities. Conclusion Healthcare expenses and lost productivity costs due to LBP were substantial, hence, there is a need for improvement of health services and policies to deal with this increasing burden of illness. We found an extensive use of diagnostic imaging, which is rather discouraged by clinical guidelines. We assume that men were experiencing high levels of back pain disability compared with women, as they presented greater absenteeism and higher lost productivity costs.
LIFE EVENTS & PSYCHIATRIC MORBIDITY IN SCHOOL CHILDREN

BACKGROUND: Psychiatric morbidity in school children can be multi-factorial. Stress in school and other life events contribute significantly to it. There are many western studies on the effects of life events on children and adolescents similar to studies on adults. Indian studies on life events have focused on their association with neurotic disorders in adults. There is paucity of literature about life events in school children and their association with psychiatric morbidity. Present study addresses this issue. AIM: To assess the association between life events and psychiatric morbidity in school children. MATERIALS AND METHODS: Two hundred and eighteen school children were selected through multistage random sampling. The study was conducted in three stages. In the first stage, all the students were administered six-item version of GHQ to screen for emotional problems. Life events were recorded using Life Events Inventory. Raven’s Progressive Matrices was administered to evaluate IQ. In the second stage, parents assessed their child’s behavior using CPMS. In the third stage, all students were subjected for detailed clinical work-up. STATISTICAL ANALYSIS: Continuous variables were analyzed using t test. RESULT: Out of 218 students 152 (69.7%) had Psychiatric illness. Majority of the students (53%) had Depression. Students with psychiatric problems had experienced more life events. Psychiatrically disturbed boys experienced more number of events than psychiatrically disturbed girls. Urban students having psychiatric problems had high life event score compared to urban students not having psychiatric problems. CONCLUSION: Psychiatrically ill students experienced more life events.
Glucose, glycolysis, and neurodegenerative diseases

Prolonged survival of a typical postmitotic neuron hinges on a balance between multiple processes, among these are a sustenance of ATP production and protection against reactive oxygen species. In neuropathological conditions, mitochondrial defects often lead to both a drop in ATP levels, as well as increase reactive oxygen species production from inefficient electron transport processes and NADPH‐oxidases activities. The former often resulted in the phenomenon of compensatory aerobic glycolysis. The latter stretches the capacity of the cell's redox buffering capacity, and may lead to damages of key enzymes involved in energy metabolism. Several recent reports have indicated that enhancing glucose availability and uptake, as well as increasing glycolytic flux via pharmacological or genetic manipulation of glycolytic enzymes, could be protective in animal models of several major neurodegenerative diseases, including Parkinson's disease, Huntington's disease, and Amyotrophic lateral sclerosis. Activation of canonical Wnt signaling, which improves disease symptoms in mouse models of Alzheimer's disease also appears to work via an elevation of glycolytic enzymes and enhance glucose metabolism. Here, I discuss these findings and the possible underlying mechanisms of how an increase in glucose uptake and glycolysis could be neuroprotective. Increased glycolytic production of ATP would help alleviate energy deficiency, and ATP's hydrotropic effect may enhance solubility and clearance of toxic aggregates prevalent in many neurodegenerative diseases. Furthermore, channeling of glucose into the Pentose Phosphate Pathway would increase the redox buffering capacity of the cell.
SON TRİMESTER NULLİPAR GEBELERDE BAZI SOSYO-DEMOGRAFİK VE OBSTETRİK ÖZELLİKLERİN PSİKOSOSYAL SAĞLIK DÜZEYİNE ETKİSİ

Amac: Gebelik; kadin yasaminda dogal bir olay olmasina ragmen neden oldugu noroendokrin ve psikososyal degisiklikler, yasamin diger donemleriyle kiyaslanamayacak derecede fazladir. Bunun yani sira gebelik kaygi ve stres olusturabilecek bircok etkenle karsilasma riskinin yuksek oldugu bir donemdir. Bu sebeple gebelerin psikososyal sagligini etkileyen faktorleri belirlemek ve sonrasinda uygun girisimlerde bulunmak onemlidir. Arastirmanin amaci son trimester nullipar gebelerde bazi sosyo-demografik ve obstetrik ozelliklerin psikososyal saglik duzeyine etkisinin incelenmesidir.Gerec ve Yontem: Arastirmanin orneklemini, Gaziantep il merkezinde bulunan bir hastanenin kadin hastaliklari ve dogum poliklinigine basvuran gebeligin son trimesterinde olan 222 nullipar gebe olusturmustur. Arastirmanin orneklem buyuklugunun belirlenmesinde, bagimli degiskene ait belirleyicilerin tanimlanmasi calismalarinda onerilen orneklem buyuklugu analizinden yararlanilmistir. Verilerin toplanmasinda arastirmaci tarafindan hazirlanan 19 soruluk anket formu ve Yildiz 2011 tarafindan gebelikte psikososyal sagligin degerlendirilmesi amaciyla gelistirilmis ve gecerlilik guvenilirligi yapilmis bir olcek olan Gebelikte Psikososyal Sagligi Degerlendirme Olcegi GPSDO kullanilmistir. GPSDO'nin toplam madde sayisi 46'dir. Olcegin 6 alt grubu vardir. Likert tipi olan GPSDO'de puanlama 1 den 5 e kadardir ve "cok fazla" 5 ile "hic" 1 arasinda degisen ifadelerle derecelendirilmistir. Arastirma oncesi arastirmanin ilgili kurumlarda yurutulmesi icin Gaziantep Şehitkâmil Devlet Hastanesinin Bashekimliginden yazili izin ve Selcuk Universitesi Etik Kurul Onayi alinmistir. Veriler 30.01. 2013-01.05. 2013 tarihleri arasinda arastirmaci tarafindan hastanenin kadin dogum poliklinigine basvuran nullipar gebelerden yuz yuze gorusme yontemi ile toplanmistir.Verilerin analizinde; sayi, yuzde, ortalama, standart sapma, Mann VVhitney U, Kruskal VVallis Varyans Analizi kullanilmistir. Istatistiksel olarak 0,05'in altindaki degerler anlamli kabul edilmistir.Bulgular ve Sonuc: GPSDO toplam puaninin 3,95±0,45 oldugu saptanmis ve gebelerin psikososyal saglik durumlarinin iyi oldugu belirlenmistir. Yas, ogrenim, calisma ve algilanilan gelir durumuna, aile tipine, en uzun yasanilan yere, evlilik kararina, es yas, ogrenim ve calisma durumuna, bebegin cinsiyetine, dusuk oykusune, ilk ve ikinci trimesterde bulanti, ikinci trimesterde kusma, ilk, ikinci ve ucuncu trimesterde uyku problemi ve kas agrilari yasama durumuna gore GPSDO toplam puani ve alt boyut puan ortalamasi bakimindan istatistiksel olarak anlamli bir fark saptanmistir p