Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrating Mesocosm Experiments with Field and Laboratory Studies to Generate Weight-of-Evidence Risk Assessments for Ecosystem Health

Assessing ecosystem health of large rivers is often complicated because these ecosystems receive multiple, interacting effluent discharges. Confounding factors, such as complicated mixing hydraulics and historical loading effects, can result in equivocal field data that lend weak inference to ecological risk assessments. Within Environment Canada's National Water Research Institute (NWRI) one approach to this problem develops a strategy that defines important mechanisms of pollutant effects through the combined use of laboratory and field measurements, riverside mesocosm experiments, and the incorporation of indicators at several trophic levels. We integrate these different types of information through weight-of-evidence postulates that provide logical guidelines for establishing causation in ecological risk assessment. Using this approach, retrospective risk assessments on a number of Canadian rivers indicates that the major effect of present effluent discharges from pulp mills has been one of nutrient enrichment and stimulation of food web productivity. The combination of field experiments with this weight-of-evidence approach yielded the scientific justification for a conceptual model that describes community shifts across a nutrient-contaminant gradient. The purpose of this is paper is to illustrate the utility of riverside mesocosms in assessing ecological impacts of point-source discharges. We conclude that the use of stream mesocosms with the weight-of-evidence approach is highly useful for establishing a mechanistic understanding of community responses to stressors at a regional scale. In addition, this approach will be useful when greater understanding of a particular class of anthropogenic stressors (e.g., specific types of effluent) is required to improve regulatory guidelines.
The Impact of Treatment Description Format on Patient Preferences for Posttraumatic Stress Disorder Treatment.

The present study examined how the format in which treatment information is presented impacts individuals' preferences for posttraumatic stress disorder (PTSD) treatments. Adults who screened positive for PTSD (N = 301) were randomized into groups to learn about five first-line treatments; participants either read sequential text descriptions or reviewed a comparison chart that presented side-by-side information. Participants rated treatment acceptability, rank ordered treatments from most to least preferred, and indicated their confidence in this ranking. Compared with participants in the text group, those in the chart group assigned more favorable acceptability ratings to prolonged exposure therapy (PE) and more moderate ratings to medications. Cognitive processing therapy was the most common first-choice treatment (43.6%). Forced-choice treatment rankings were similar across conditions, although participants in the chart group ranked PE more favorably than those in the text group, odds ratio (OR) = 0.54, 95% CI [0.35, 0.82], p = . 004. Confidence in treatment rankings did not differ across conditions. The results suggest that perceptions of treatment acceptability can be influenced by the format in which treatment information is presented. In settings where the goal is to increase treatment acceptability, side-by-side formats may offer an advantage over sequential descriptions of each treatment.
Biological and health-related effects of weak static magnetic fields (≤ 1 mT) in humans and vertebrates: A systematic review

Background There is a rapid development in technologies that generate weak static magnetic fields (SMF) including high-voltage direct current (HVDC) lines, systems operating with batteries, such as electric cars, and devices using permanent magnets. However, few reviews on the effects of such fields on biological systems have been prepared and none of these evaluations have had a particular focus on weak SMF (≤ 1 mT). The aim of this review was to systematically analyze and evaluate possible effects of weak SMF (≤ 1 mT) on biological functioning and to provide an update on the current state of research. Methods This review was prepared in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) statement. Methodological limitations in individual studies were assessed using the Office of Health Assessment and Translation (OHAT) Risk-of-Bias Rating Tool. Results Eleven studies fulfilled the eligibility criteria and were included in this review. All included studies were experimental animal studies as no human studies were among the eligible articles. Eight of the eleven studies reported responses of rat, rabbits and quails to weak SMF exposure that were expressed as altered melatonin biosynthesis, reduced locomotor activity, altered vasomotion and blood pressure, transient changes in blood pressure-related biochemical parameters, or in the level of neurotransmitters and increases in enzyme activities. It remained largely unclear from the interpretation of the results whether the reported effects in the evaluated studies were beneficial or detrimental for health. Conclusion The available evidence from the literature reviewed is not sufficient to draw a conclusion for biological and health-related effects of exposure to weak SMF. There was a lack of homogeneity regarding the exposed biological systems and the examined endpoints as well as a lack of scientific rigor in most reviewed studies which lowered credibility in the reported results. We therefore encourage further and more systematic research in this area. Any new studies should particularly address effects of exposure to SMF on biological functioning in humans to evaluate whether SMF pose a risk to human health.
Considerations for and Guidance to Testing and Evaluating Migration/Release of Nanoparticles from Polymer Based Nanocomposites

The use of nanoadditives in food contact materials requires risk assessment to ensure consumers’ safety. The evaluation of health risk is based on the combination of two elements: hazard and exposure. For nanomaterials (NM) used as additives in nanocomposites, the exposure is directly linked to the level of migration or release of the NM into the food. In principle, appropriate methods for experimental determination and theoretical estimation of migration are available but need diligent considerations to avoid erroneous conclusions from the measured data. We propose a comprehensive test scheme based on these methods, starting with characterization of the nanomaterial itself and when incorporated in the polymer. These data form the basis for making a decision whether migration of the NM can be excluded by migration theoretical considerations or if experimental migration testing and/or abrasion testing for mechanical release should be carried out. Guidance to and considerations for each of these steps and regarding the applicable methods are discussed. In conclusion, the results will provide a basis for risk assessment, either directly when exposure of consumers to the nanomaterials can be excluded or will be very low or, in the case of evidenced exposure, in combination with then needed toxicological data.
Interleukin‐9 serves as a key link between systemic inflammation and angiogenesis in psoriasis

Psoriasis is a T helper cell‐mediated chronic immune‐mediated inflammatory disease affecting mainly the skin, although systemic pathological effects are also observed. Cytokine‐mediated interaction between T lymphocytes and keratinocytes lead to excessive proliferation of keratinocytes, which in turn leads to formation of a proinflammatory milieu and finally to psoriatic plaque formation.
A Clinical Perspective on the Antimicrobial Resistance Spectrum of Uropathogens in a Romanian Male Population

Considering urinary tract infections (UTIs), a significant public health problem with negligible recent research, especially on the male eastern European population, we aimed to determine the antimicrobial resistance rates of uropathogens for the most commonly used antibiotics in urological practice in our country. We conducted a multicenter retrospective study in two different teaching hospitals in Romania, analyzing urine samples from 7719 patients to determine the frequency of incriminating pathogens and their resistance to different antibiotics, in a comparative approach. We determined Escherichia coli (35.98%) to be the most common pathogen with the highest sensitivity to amikacin (S = 91.72%), meropenem (S = 97.17%) and fosfomycin (S = 86.60%) and important resistance to amoxicillin-clavulanic ac. (R = 28.03%) and levofloxacin (R = 37.69%), followed by Klebsiella spp. (22.98%) with the highest sensitivity to amikacin (S = 78.04%) and meropenem (S = 81.35%) and important resistance to amoxicillin-clavulanic ac. (R = 65.58%) and levofloxacin (R = 45.36%); the most frequent Gram-positive pathogen was Enterococcus spp. (19.73%) with the highest sensitivity for vancomycin (S = 93.75%) and fosfomycin (S = 87.5%) and considerable resistance to penicillin (R = 33.52%) and levofloxacin (R = 42.04%). The findings are an important tool in managing UTIs and should be acknowledged as reference research not only for clinicians from Romania but for all physicians treating male UTIs.
Structure and Dynamics of Spider Silk Studied with Solid-State Nuclear Magnetic Resonance and Molecular Dynamics Simulation

This review will introduce very recent studies using solid-state nuclear magnetic resonance (NMR) and molecular dynamics (MD) simulation on the structure and dynamics of spider dragline silks conducted by the author’s research group. Spider dragline silks possess extraordinary mechanical properties by combining high tensile strength with outstanding elongation before breaking, and therefore continue to attract attention of researchers in biology, biochemistry, biophysics, analytical chemistry, polymer technology, textile technology, and tissue engineering. However, the inherently non-crystalline structure means that X-ray diffraction and electron diffraction methods provide only limited information because it is difficult to study the molecular structure of the amorphous region. The most detailed picture of the structure and dynamics of the silks in the solid state experimentally have come from solid-state NMR measurements coupled with stable isotope labeling of the silks and the related silk peptides. In addition, combination of solid-state NMR and MD simulation was very powerful analytical tools to understand the local conformation and dynamics of the spider dragline silk in atomic resolution. In this review, the author will emphasize how solid-state NMR and MD simulation have contributed to a better understanding of the structure and dynamics in the spider dragline silks.
The Cardio-Med survey tool: development and pilot validation of a FFQ in a multicultural cardiology cohort

Abstract Objective: (i) Describe the development of a multipurpose Cardio-Med survey tool (CMST) comprising a semi-quantitative FFQ designed to measure dietary intake in multicultural patients with or at high risk of CVD and (ii) report pilot evaluation of test–retest reliability and validity of the FFQ in measuring energy and nutrient intakes. Design: The CMST was developed to identify CVD risk factors and assess diet quality over 1 year using an FFQ. Design of the ninety-three-item FFQ involved developing food portion photographs, and a list of foods appropriate for the Australian multicultural population allowing the capture of adherence to a Mediterranean diet pattern. The FFQ was administered twice, 2 weeks apart to assess test–retest reliability, whilst validity was assessed by comparison of the FFQ with a 3-d food record (3DFR). Setting: The Northern Hospital and St Vincent’s Hospital, Melbourne, Australia. Participants: Thirty-eight participants aged 34–81 years with CVD or at high risk. Results: Test–retest reliability of the FFQ was good: intraclass correlation coefficient (ICC) ranged from 0·52 (Na) to 0·88 (alcohol) (mean 0·79), with energy and 70 % of measured nutrients being above 0·75. Validity was moderate: ICC ranged from 0·08 (Na) to 0·94 (alcohol) (mean 0·59), with energy and 85 % of measured nutrients being above 0·5. Bland–Altman plots demonstrated good levels of agreement between the FFQ and 3DFR for carbohydrates, protein, alcohol, vitamin D and Na. Conclusions: The CMST FFQ demonstrated good test–retest reliability and moderate validity for measuring dietary energy and nutrients in a multicultural Australian cardiology population.
Intake of Spineless Cladodes of Opuntia ficus-indica During Late Pregnancy Improves Progeny Performance in Underfed Sheep

Simple Summary Plants in the Opuntia genus are abundant and can be used as a feed supplement because they are highly digestible and can provide water and energy. We fed sheep during late gestation with alfalfa (Control), Opuntia (Opuntia) or protein-enriched Opuntia (E-Opuntia) and measured milk yield and postnatal growth in the progeny. Birth weight did not differ among treatments (p > 0.05) but progeny from E-Opuntia grew faster (p < 0.01) and were heavier at weaning (p < 0.05), despite the fact that Control ewes produced more milk (p < 0.05). Feeding ewes with Opuntia (protein enriched or not) during the last third of gestation is an option for reducing production costs in underfed females managed under extensive conditions in arid and semiarid regions. Abstract The present study tested whether feeding ewes during the last third of pregnancy with cladodes of Opuntia (untreated or protein-enriched), as an alternative to alfalfa hay, would improve milk yield as well as the pre- and post-natal growth of their lambs. Sixty mature Rambouillet ewes and their progeny were randomly allocated among three nutritional treatments: (i) Control, fed alfalfa; (ii) Opuntia, fed untreated cladodes; (iii) E-Opuntia, fed protein-enriched cladodes (pre-treated with urea and ammonium sulphate). Birth weight did not differ among treatments (p > 0.05) but Control ewes produced more milk than both groups of Opuntia-fed ewes (p < 0.05). However, milk yield was not related to the growth of the progeny (p > 0.05) because lambs from E-Opuntia-fed ewes grew faster (p < 0.01) and were heavier at weaning (p < 0.05) than lambs from the other two groups. We conclude that Opuntia (with or without protein enrichment) can be used as an alternative to alfalfa hay for feeding ewes during the last third of pregnancy and therefore reduce production costs under extensive conditions in arid and semiarid regions. Moreover, protein-enriched Opuntia appears to improve postnatal lamb growth.
Chemical Markers to Distinguish the Homo- and Heterozygous Bitter Genotype in Sweet Almond Kernels

Bitterness in almonds is controlled by a single gene (Sk dominant for sweet kernel, sk recessive for bitter kernel) and the proportions of the offspring genotypes (SkSk, Sksk, sksk) depend on the progenitors’ genotype. Currently, the latter is deduced after crossing by recording the phenotype of their descendants through kernel tasting. Chemical markers to early identify parental genotypes related to bitter traits can significantly enhance the efficiency of almond breeding programs. On this basis, volatile metabolites related to almond bitterness were investigated by Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry coupled to univariate and multivariate statistics on 244 homo- and heterozygous samples from 42 different cultivars. This study evidenced the association between sweet almonds’ genotype and some volatile metabolites, in particular benzaldehyde, and provided for the first time chemical markers to discriminate between homo- and heterozygous sweet almond genotypes. Furthermore, a multivariate approach based on independent variables was developed to increase the reliability of almond classification. The Partial Least Square-Discriminant Analysis classification model built with selected volatile metabolites that showed discrimination capacity allowed a 98.0% correct classification. The metabolites identified, in particular benzaldehyde, become suitable markers for the early genotype identification in almonds, while a DNA molecular marker is not yet available.
Identification of Proteus genomic island 2 variants in two clonal Proteus mirabilis isolates with coexistence of a novel genomic resistance island PmGRI1.

OBJECTIVES To characterize the MDR genomic islands (GIs) in Proteus mirabilis isolates. METHODS Two P. mirabilis strains (C55 and C74) of chicken origin were subjected to WGS (HiSeq and PacBio) and the MDR GIs were determined. RESULTS P. mirabilis strains C55 and C74 are clonal strains and harbour different Proteus genomic island 2 (PGI2) variants (PGI2-C55 and PGI2-C74). The MDR region of PGI2-C55 is composed of two class 1 integrons, separated by a region containing seven copies of IS26 and eight resistance genes, including blaCTX-M-3 and fosA3. The region in PGI2-C74 is a complete In4-type class 1 integron, harbouring five gene cassettes (dfrA16, blaCARB-2, aadA2, cmlA1 and aadA1). In addition, C55 and C74 carry an SXT/R391 integrative and conjugative element (ICEPmiJpn1), harbouring blaCMY-2, and a novel 50.46 kb genomic resistance island named PmGRI1-C55. PmGRI1-C55 harbours a tyrosine-type recombinase/integrase that might be responsible for the integration of PmGRI1-C55 at the 3' end of tRNA-Sec. It carries an MDR region derived from Tn2670 that harbours a Tn21 region and carries six resistance genes (catA1, blaTEM-1b, aphA1a, sul2, strA and strB). Blast analysis showed diverse PmGRI1 variants in P. mirabilis and Escherichia coli strains. CONCLUSIONS The finding of the two new PGI2 variants highlights that the homologous recombination between shared components of class 1 integrons and transposition by IS26 promote the diversity of MDR regions in PGI2. PmGRI1 is a new GI that carries various resistance genes identified in P. mirabilis and E. coli.
Effect of plant spacing on growth and yield of lettuce (Lactuca sativa L.) in a soilless production system

Abstract Lettuce production in re-circulating hydroponic systems is done on a limited scale in South Africa with conflicting information on the recommended spacing for optimal yield and quality in such systems. The development and yield of four lettuce (Lactuca sativa L.) cultivars ‘NIZ 44–675’, ‘Nou-gatine’, ‘Tango’ and ‘Natividad’ at five different inter- and intra-row spacings namely: 10x20 (50 plants m-2), 10x25 (40 plants m-2), 15×20 (30 plants m-2), 20×20 (25 plants m-2) and 20x25 (20 plants m-2) cm were evaluated during May/June 2008. The experimental layout was a randomized complete block design and each treatment combination was replicated three times. There was a significant interaction between spacing and cultivar regarding leaf fresh and dry mass while all cultivars reacted similarly to different spacings on other yield parameters. Plant population significantly affected plant height, fresh and dry leaf mass, leaf area and leaf number m-2, with significantly higher values of all variables at the closest spacing (50 plants m-2). The results indicate that an increase in plant population results in a significant increase in yield and yield components of leafy lettuce, with all cultivars producing the highest yield at a spacing of 50 plants m- 2 during winter production. Cultivar differences observed showed that ‘NIZ 44–675’ and ‘Tango’ performed better than ‘Nougatine’ and ‘Natividad’, mostly due to higher leaf area, fresh mass, plant height and leaf number.
Particle penetration through rectangular-shaped cracks

An outdoor-to-indoor particle penetration experiment was undertaken to validate an air infiltration flow and particle penetration model that simulates particle transport through cracks between a pair of parallel plates. Results indicated that the infiltration flow model agreed with experimental observations, and the infiltration flow field could be assumed laminar for typical outdoor-to-indoor differential pressures (1 to 15 Pa). Observations of the particle deposition pattern on crack surfaces indicated that gravitational sedimentation was the dominant deposition mechanism for 1.4 µm particles. Particle penetration coefficient (Pp) increased monotonously with increasing differential pressure and crack height (H), and decreased monotonously with increasing crack length (L). For H > 0.305 mm, and L 0.406 mm cracks under the outdoor-to-indoor differential pressures tested. The part.
Effects of 3,4-dihydroxybenzoic acid on tobacco (Nicotiana tabacum L.) cultured in vitro. Growth regulation in callus and organ cultures

ABSTRACT The influence of 3,4-dihydroxybenzoic acid (protocatechuic acid), a naturally occurring benzoic acid derivative, on tobacco (Nicotiana tabacum L.) cell and tissue cultures was examined. The response to 0.1, 10 and 1000 µM 3,4-dihydroxybenzoic acid was tested with regards to cell proliferation in leaf explants, callus growth and shoot formation. Effects on shoot and root growth in micropropagated plants were also analysed. The highest concentration of 3,4-dihydroxybenzoic acid strongly inhibited the proliferation of leaf tissues, callus growth, shoot regeneration and root growth in micropropagated plants. On the contrary, the lowest concentration (0.1 µM) showed auxin-like activity by stimulating cell dedifferentiation, callus induction and rooting of leaf tissues. The presence of auxins and cytokinins in the media contrasted 3,4-dihydroxybenzoic acid inhibition of callus growth at all tested concentrations.
Racial/Ethnic Disparities in the Relationship of Alcohol-Related Injury and Perceived Driving Under the Influence from Hours of Exposure to High Blood Alcohol Concentration: Data From Four US National Alcohol Surveys (2000-2015).

AIMS To analyze racial/ethnic disparities in risk of two alcohol-related events, alcohol-related injury and self-reported perceived driving under the influence (DUI) from hours of exposure to an elevated blood alcohol concentration (BAC). METHODS Risk curves for the predicted probability of these two outcomes from the number of hours of exposure to a BAC ≥ 0.08 mg% in the past year were analyzed separately for whites, blacks and Hispanics in a merged sample of respondents from four US National Alcohol Surveys (2000-2015). RESULTS Hours of exposure to a BAC ≥ 0.08 showed a stronger association with perceived DUI than with alcohol-related injury for all racial/ethnic groups. Greater risk was found for whites than blacks or Hispanics for outcomes at nearly all BAC exposure levels, and most marked at the highest level of exposure. Risk of both outcomes was significant for whites at all exposure levels, but small for alcohol-related injury. Little association was found for alcohol-related injury for blacks or Hispanics. For perceived DUI, risk for blacks was significantly elevated at lower levels of exposure, while risk for Hispanics was significantly elevated beginning at 30 h of exposure. CONCLUSIONS Findings showed racial/ethnic differences in risk of alcohol-related injury and perceived DUI from hours of exposure to elevated BAC. Risk increased at relatively low levels of exposure to a BAC ≥ 0.08, especially for whites, highlighting the importance of preventive efforts to reduce harmful outcomes for moderate drinkers.