Miracle-dz/UniFAR-FTUs huggingface dataset:
Immobilization of urease enzyme on chitosan/polyvinyl alcohol electrospun nanofibers

Electrospun nanofibers have gained much attention for enzyme immobilization due to their high surface‐to‐volume ratio. In this study, urease was immobilized on chitosan/poly(vinyl alcohol) (PVA) nanofibers by both adsorption and crosslinking methods. In order to obtain nanofibers with more desirable properties, solutions with different ratios of chitosan and PVA were electrospun and crosslinked using glutaraldehyde. Comparing SEM images of the nanofibers, before and after immersing them in phosphate buffer, it was shown that higher chitosan content leads to more stable fibers. So, the solution with the chitosan to PVA ratio of 40:60 was used for enzyme immobilization. Then, the effects of initial protein concentration, temperature, incubation time, and method of immobilization were investigated to reach the highest enzyme activity. Under similar immobilization conditions, covalently immobilized urease showed higher activity, compared to uncrosslinked immobilized enzyme. Besides, it retained 30% of its initial activity after 10 times usage. So, this method was chosen for further investigation. Not only the activity of the immobilized enzyme was much higher than the free enzyme in a wide range of pH and temperature, but also stability of the immobilized enzyme was improved. Immobilized urease was then used to remove thiourea which is a toxic compound. Findings indicated 60% hydrolysis of initial thiourea in 12 h. In conclusion, the findings showed that chitosan/PVA nanofibers are suitable candidates for the immobilization of urease.
Effect of 8-week intake of the n-3 fatty acid-rich perilla oil on the gut function and as a fuel source for female athletes: a randomised trial

Abstract Previous studies have examined the effects of n-3 fatty acid intake in supplement form or fish oil capsules, but there are few studies based on other foods. Perilla oil is a traditional Japanese seed oil rich in n-3 fatty acids. This randomised trial aimed to determine the appropriate n-3 fatty acid dose through consumption of perilla oil, which improves gut function and microbiota in trained athletes, and the amount of fat fuel required to provide energy to athletes involved in high-intensity training to improve athletic performance. Thirty-six female athletes training six times per week were randomly assigned to three groups according to perilla oil intake: 9 g/d (high oil intake (HOI)), 3 g/d (low oil intake (LOI)) and placebo-supplementation (PLA) groups. The HOI and LOI groups had perilla oil-containing jelly and the PLA group had placebo jelly for 8 weeks. Gut microbiota, constipation score and urinary biochemical index were measured pre- and post-intervention. The spoilage bacteria, Proteobacteria, significantly decreased (P = 0·036, d = 0·53), whereas Butyrate-producing bacteria, Lachnospiraceae, significantly increased (P = 0·007, d = 1·2) in the HOI group. Urinary indoxyl sulphate significantly decreased in the HOI group only (P = 0·010, d = 0·82). Changes in the constipation score were significantly lower in the HOI group (P = 0·020) and even lower in the LOI group (P = 0·073) than in the PLA group; there were significant differences between groups (P = 0·035). Therefore, perilla oil intake may improve gut function and microbiota in athletes, with higher doses resulting in further improvement.
Active conductive head cooling of normal and infarcted brain: A magnetic resonance spectroscopy imaging study

Active conductive head cooling is a simple and non-invasive intervention that may slow infarct growth in ischemic stroke. We investigated the effect of active conductive head cooling on brain temperature using whole brain echo-planar spectroscopic imaging. A cooling cap (WElkins Temperature Regulation System, 2nd Gen) was used to administer cooling for 80 minutes to healthy volunteers and chronic stroke patients. Whole brain echo-planar spectroscopic imaging scans were obtained before and after cooling. Brain temperature was estimated using the Metabolite Imaging and Data Analysis System software package, which allows voxel-level temperature calculations using the chemical shift difference between metabolite (N-acetylaspartate, creatine, choline) and water resonances. Eleven participants (six healthy volunteers, five post-stroke) underwent 80 ± 5 minutes of cooling. The average temperature of the coolant was 1.3 ± 0.5°C below zero. Significant reductions in brain temperature (ΔT = –0.9 ± 0.7°C, P = 0.002), and to a lesser extent, rectal temperature (ΔT = –0.3 ± 0.1°C, P = 0.03) were observed. Exploratory analysis showed that the occipital lobes had the greatest reduction in temperature (ΔT = –1.5 ± 1.2°C, P = 0.002). Regions of infarction had similar temperature reductions to the contralateral normal brain. Future research could investigate the feasibility of head cooling as a potential neuroprotective strategy in patients being considered for acute stroke therapies.
Macrophage-like Cells Characterized by En Face Optical Coherence Tomography was Associated with Fluorescein Vascular Leakage in Behçet’s Uveitis

ABSTRACT Purpose To investigate the features of macrophage-like cell (MLC) characterized by en face optical coherence tomography (OCT) in Behçet’s uveitis (BU). Methods The extent of fluorescein vascular leakage (FVL) was graded on a scale of 0-3 (0=none, 1=mild, 2=moderate, 3=severe) for the optic nerve head (ONH), macula and peripheral retina. The 3μm en face OCT slabs on inner limiting membrane of ONH or macular region was used to visualize the MLCs. Results The MLC densities of BU group in ONH and macular region were significantly higher than the control group (both p<0.001). The ONH and macular MLC density were significantly higher in eyes with higher FVL grade and they were positively correlated with FVL score (all p<0.001). Conclusion MLC density was elevated in Behçet’s uveitis and it may serve as a noninvasive indicator for the severity of fluorescein leakage and retinal inflammation in Behcet’s uveitis.
A naturalistic study of passenger seating position, posture, and restraint use in second-row seats

Abstract Objective The objective of the current study was to increase scientific understanding of rear-seat passenger seating position, postures, CRS use, and belt use through a naturalistic study. A secondary objective was to compare data from vehicles used in ride-hailing with data from other vehicles. Method Video cameras were installed in the passenger cabins of the vehicles of 75 drivers near the center of the windshield. The video data were downloaded after the vehicles were operated by their owners for two weeks. Video frames were sampled from near the ends and in the middle of each trip, and at five-minute intervals in trips longer than 15 min. A total of 7,323 frames with second-row passengers were manually coded. Results A total of 444 unique second-row passengers were identified in video frames from 1,188 trips taken in 65 of the 75 vehicles in the study. Two of the vehicles that were driven for commercial ride-hailing during the study period accounted for 199 (45%) of the passengers. Considering multiple passengers in some trips, a total of 1,899 passenger-trips were identified. For passengers not using child restraint systems (CRS), the belt use rate was 65% in the non-ride-hailing vehicles versus 32% among passengers in the ride-hailing vehicles. No CRS use was observed in the ride-hailing vehicles. Among children using backless boosters, the shoulder belt was lateral to the clavicle or under the arm in 26% of frames. Among belted passengers not using CRS, the belt was lateral to the clavicle or on the neck about 6% of the time. Belted passengers not using CRS were observed leaning to the left or right about 27% of the time, with leaning away from the shoulder belt more common than leaning into the belt. Conclusions This study is the first to report seating position, posture, and belt fit observations for a large naturalistic sample of second-row passengers that includes adult occupants. The data suggest that low rear seat belt use rates remain a concern, particularly in ride-hailing vehicles. Non-nominal belt placement and posture may also be common in second-row seating positions.
Views of young people with psychosis on using virtual reality assisted therapy. A qualitative study

Service disengagement is a challenge in young individuals struggling with psychosis. Combining cognitive behavioural therapy for psychosis (CBTp) with virtual reality (VR) has proven acceptable and potentially effective for symptoms and social functioning in adults with psychosis. However, studies focusing on young adolescents are lacking. The aim of the present study was to investigate the acceptability of VR‐assisted CBTp among adolescents with psychosis.
Predicting COVID‐19 and seasonal influenza vaccine uptake: The impact of fear and vasovagal symptoms

Abstract Vaccines are vital to protecting health. However, fear and experiencing vasovagal symptoms (e.g., dizziness) are deterrents to medical procedures. Thus, study aims were to test (1) if vaccine relevant fears predict vasovagal symptoms during or following seasonal influenza vaccination and (2) if vaccine relevant fears and vasovagal symptoms predict seasonal influenza and COVID‐19 vaccine uptake. Using a prospective design, 1077 participants recruited online completed surveys during Oct 2019 assessing vaccine relevant fears, and May–June 2020 assessing 2019–2020 seasonal influenza vaccine uptake, ratings of vasovagal symptoms, and seasonal influenza and COVID‐19 vaccination intention. A behavioral follow up assessing 2020–2021 seasonal influenza and COVID‐19 vaccine uptake took place June–July 2021. Heightened vaccine relevant fears predicted reduced 2019–2020 seasonal influenza vaccine uptake and greater vasovagal symptoms among those who did receive a seasonal influenza vaccine. Serial mediation analyses identified significant indirect effects with greater vaccine relevant fears reducing 2020–2021 seasonal influenza vaccine uptake through intention and reducing COVID‐19 vaccine uptake through vasovagal symptoms and intention. Intervention research to reduce fear and prevent vasovagal symptoms to support vaccine uptake is warranted.
Predictors of rapid eye movement sleep behavior disorder in patients with Parkinson’s disease based on random forest and decision tree

Background and objectives Sleep disorders related to Parkinson’s disease (PD) have recently attracted increasing attention, but there are few clinical reports on the correlation of Parkinson’s disease patients with rapid eye movement (REM) sleep behavior disorder (RBD). Therefore, this study conducted a cognitive function examination for Parkinson’s disease patients and discussed the application effect of three algorithms in the screening of influencing factors and risk prediction effects. Methods Three algorithms (logistic regression, machine learning-based regression trees and random forest) were used to establish a prediction model for PD-RBD patients, and the application effects of the three algorithms in the screening of influencing factors and the risk prediction of PD-RBD were discussed. Results The subjects included 169 patients with Parkinson’s disease (Parkinson’s disease with RBD [PD-RBD] = 69 subjects; Parkinson’s disease without RBD [PD-nRBD] = 100 subjects). This study compared the predictive performance of RF, decision tree and logistic regression, selected a final model with the best model performance and proposed the importance of variables in the final model. After the analysis, the accuracy of RF (83.05%) was better than that of the other models (decision tree = 75.10%, logistic regression = 71.62%). PQSI, Scopa-AUT score, MoCA score, MMSE score, AGE, LEDD, PD-course, UPDRS total score, ESS score, NMSQ, disease type, RLSRS, HAMD, UPDRS III and PDOnsetage are the main variables for predicting RBD, along with increased weight. Among them, PQSI is the most important factor. The prediction model of Parkinson’s disease RBD that was established in this study will help in screening out predictive factors and in providing a reference for the prognosis and preventive treatment of PD-RBD patients. Conclusions The random forest model had good performance in the prediction and evaluation of PD-RBD influencing factors and was superior to decision tree and traditional logistic regression models in many aspects, which can provide a reference for the prognosis and preventive treatment of PD-RBD patients.
Secretory Phospholipase A2-IIA and Cardiovascular Disease A Mendelian Randomization Study

Holmes, Michael V; Simon, Tabassome; Exeter, Holly J; Folkersen, Lasse; Asselbergs, Folkert W; Guardiola, Montse; Cooper, Jackie A; Palmen, Jutta; Hubacek, Jaroslav A; Carruthers, Kathryn F; +103 more. Horne, Benjamin D; Brunisholz, Kimberly D; Mega, Jessica L; van Iperen, Erik PA; Li, Mingyao; Leusink, Maarten; Trompet, Stella; Verschuren, Jeffrey JW; Hovingh, G Kees; Dehghan, Abbas; Nelson, Christopher P; Kotti, Salma; Danchin, Nicolas; Scholz, Markus; Haase, Christiane L; Rothenbacher, Dietrich; Swerdlow, Daniel I; Kuchenbaecker, Karoline B; Staines-Urias, Eleonora; Goel, Anuj; van ’t Hooft, Ferdinand; Gertow, Karl; de Faire, Ulf; Panayiotou, Andrie G; Tremoli, Elena; Baldassarre, Damiano; Veglia, Fabrizio; Holdt, Lesca M; Beutner, Frank; Gansevoort, Ron T; Navis, Gerjan J; Mateo Leach, Irene; Breitling, Lutz P; Brenner, Hermann; Thiery, Joachim; Dallmeier, Dhayana; Franco-Cereceda, Anders; Boer, Jolanda MA; Stephens, Jeffrey W; Hofker, Marten H; Tedgui, Alain; Hofman, Albert; Uitterlinden, André G; Adamkova, Vera; Pitha, Jan; Onland-Moret, N Charlotte; Cramer, Maarten J; Nathoe, Hendrik M; Spiering, Wilko; Klungel, Olaf H; Kumari, Meena; Whincup, Peter H; Morrow, David A; Braund, Peter S; Hall, Alistair S; Olsson, Anders G; Doevendans, Pieter A; Trip, Mieke D; Tobin, Martin D; Hamsten, Anders; Watkins, Hugh; Koenig, Wolfgang; Nicolaides, Andrew N; Teupser, Daniel; Day, Ian NM; Carlquist, John F; Gaunt, Tom R; Ford, Ian; Sattar, Naveed; Tsimikas, Sotirios; Schwartz, Gregory G; Lawlor, Debbie A; Morris, Richard W; Sandhu, Manjinder S; Poledne, Rudolf; Maitland-van der Zee, Anke H; Khaw, Kay-Tee; Keating, Brendan J; van der Harst, Pim; Price, Jackie F; Mehta, Shamir R; Yusuf, Salim; Witteman, Jaqueline CM; Franco, Oscar H; Jukema, J Wouter; de Knijff, Peter; TybjaergHansen, Anne; Rader, Daniel J; Farrall, Martin; Samani, Nilesh J; Kivimaki, Mika; Fox, Keith AA; Humphries, Steve E; Anderson, Jeffrey L; Boekholdt, S Matthijs; Palmer, Tom M; Eriksson, Per; Paré, Guillaume; Hingorani, Aroon D; Sabatine, Marc S; Mallat, Ziad; Casas, Juan P; Talmud, Philippa J; (2013) Secretory phospholipase A(2)-IIA and cardiovascular disease: a mendelian randomization study. Journal of the American College of Cardiology, 62 (21). pp. 1966-1976. ISSN 0735-1097 DOI: https://doi.org/10.1016/j.jacc. 2013.06. 044
Title: The Efficacy of Direct Mail, Patient Navigation, and Incentives for Increasing Mammography and Colonoscopy in the Medicaid Population: A Randomized Controlled Trial

Title Page Title: The Efficacy of Direct Mail, Patient Navigation, and Incentives for Increasing Mammography and Colonoscopy in the Medicaid Population: A Randomized Controlled Trial Authors: Jonathan S. Slater, Michael J. Parks, Christina L. Nelson, Kelly D. Hughes Minnesota Department of Health, Cancer Control Section 85 East 7th Place, P.O. Box 64882, St. Paul. MN 55164-0882 Running Title: RCT to increase cancer screening among Medicaid clients Abbreviations list: breast cancer (BC); colorectal cancer (CRC); federal poverty level (FPL); National Breast and Cervical Cancer Early Detection Program (NBCCEDP): Minnesota Department of Health (MDH); Minnesota Department of Human Services (MDHS); Minnesota Health Care Programs (MHCP); Current Procedural Technology (CPT); fecal occult immunochemical tests (FIT)
OP-SCAN160103 1853..1862

Dogs are hypersocial with humans, and their integration into human social ecology makes dogs a unique model for studying cross-species social bonding. However, the proximal neural mechanisms driving dog–human social interaction are unknown. We used functional magnetic resonance imaging in 15 awake dogs to probe the neural basis for their preferences for social interaction and food reward. In a first experiment, we used the ventral caudate as a measure of intrinsic reward value and compared activation to conditioned stimuli that predicted food, praise or nothing. Relative to the control stimulus, the caudate was significantly more active to the reward-predicting stimuli and showed roughly equal or greater activation to praise vs food in 13 of 15 dogs. To confirm that these differences were driven by the intrinsic value of social praise, we performed a second imaging experiment in which the praise was withheld on a subset of trials. The difference in caudate activation to the receipt of praise, relative to its withholding, was strongly correlated with the differential activation to the conditioned stimuli in the first experiment. In a third experiment, we performed an out-of-scanner choice task in which the dog repeatedly selected food or owner in a Y-maze. The relative caudate activation to foodand praise-predicting stimuli in Experiment 1 was a strong predictor of each dog’s sequence of choices in the Y-maze. Analogous to similar neuroimaging studies of individual differences in human social reward, our findings demonstrate a neural mechanism for preference in domestic dogs that is stable within, but variable between, individuals. Moreover, the individual differences in the caudate responses indicate the potentially higher value of social than food reward for some dogs and may help to explain the apparent efficacy of social interaction in dog training.
Sjögren's Syndrome, Salivary Glands Bioavailability in Normal, but Not in Epithelial CXCR3-B Regulates Chemokines

Expression of CXCR3-targeting chemokines have been demonstrated in several diseases, suggesting a critical role for CXCR3 in recruiting activated T cells to sites of immune-mediated inﬂammation. Sjo¨gren’s syndrome (SS) is an autoimmune disease char-acterized by a mononuclear cell inﬁltrate of activated T cells around the duct in the salivary gland. Analysis of minor salivary gland biopsy specimens from 20 healthy subjects and 18 patients with primary SS demonstrated that CXCR3, in particular, the B form of this receptor, is constitutively expressed by human salivary gland epithelial cells. Salivary gland epithelial cell cultures demonstrated that CXCR3 participate in removing relevant amount of agonists from the supernatant of exposed cells without mediating calcium ﬂux or chemotaxis while retaining the ability to undergo internalization. Although in normal salivary gland epithelial cells, CXCR3 behaves as a chemokine-scavenging receptor, its role in SS cells is functionally impaired. The impairment of this scavenging function might favor chemotaxis, leading to heightened immigration of CXCR3-positive T lymphocytes. These ﬁndings suggest that epithelial CXCR3 may be involved in postsecretion regulation of chemokine bioavailability. They also support a critical role for CXCR3 in the pathogenesis of SS and identify its agonists as potential therapeutic targets. The Journal of Immunology, 2006, 176: 2581–2589.
From web-based to mobile: Experiences of developing a Personally Controlled Management System

Objective/Aims: Personally controlled health management systems (PCHMS) in mobile technologies offer a new platform for personal health care. The objective of this study was to develop a mobile version of “Healthy.me”, an existing web-based PCHMS, and analyse its usability. Method: The mobile extension of the PCHMS was iteratively designed from its web-based version and evaluated over a period of 6 months. 17 participants completed a 30-minute five-part usability study incorporating the think aloud protocol, task activities, card ranking exercises, interviews, and a technology acceptance questionnaire measuring the “perceived usefulness” and “perceived ease of use” of the mobile version. Results: A mobile version of the PCHMS from its original web-based conception was successfully developed. According to their ratings on the technology acceptance questionnaire, participants found the overall mobile application useful, easy to use, and that there is strong intention to use the mobile application to manage their health. Conclusion: Providing a PCHMS in both web-based and mobile-based platform that is well accepted by consumers should allow more people to have convenient access to their health information, selfmanagement tools and thus improve their overall day-to-day health management. The user experience on smartphone should be carefully considered when transferring a PCHMS from traditional web-based to mobile application.
The rapid appearance of herbicide-resistant weeds combined with a lack of novel herbicides

Short title: Development of CESA3 dual-herbicide resistance 1 2 Corresponding author: 3 Lieven De Veylder 4 VIB Center for Plant Systems Biology 5 Ghent University, Department of Plant Biotechnology and Bioinformatics 6 Technologiepark 927 7 B-9052 Gent (Belgium) 8 Tel. : +32 9 3313961; Fax: +32 9 3313809; E-mail: lieven.deveylder@psb.vib-ugent.be 9 10 Genome Editing-Based Engineering of CESA3 Dual Cellulose-Inhibitor-Resistant 11 Plants 12 Zhubing Hu,a,b,c,d Teng Zhang,a,d Debbie Rombaut,b,c Ward Decaestecker,b,c Aiming 13 Xing, Sam D’Haeyer, Rene Höfer, Ilse Vercauteren, Mansour Karimi, Thomas 14 Jacobs, and Lieven De Veylder, 15 Key Laboratory of Plant Stress Biology, State Key Laboratory of Cotton Biology, School of 16 Life Sciences, Henan University, 475001 Kaifeng, China 17 Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, 18 Belgium 19 Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium 20 College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, China 21 Discovery Sciences, VIB, 9052 Ghent, Belgium 22 23 24 ORCID IDs: 0000-0002-5394-9864 (Z.H.); 0000-0003-0247-3189 (W.D.); 0000-0003-0964-1253 25 (R.H); 0000-0001-9561-8468 (I.V); 0000-0002-0246-9318 (M.K.); 0000-0002-5408-492X (T.J.); 26 0000-0003-1150-4426 (L.D.V.) 27
Cloning and Location of a Gene Governing Lysine g-Aminotransferase, an Enzyme Initiating 1-Lactam Biosynthesis in Streptomyces spp.

In actinomycetes that produce P-lactam antibiotics of the cephem type, lysine r-aminotransferase is the initial enzyme in the conversion of lysine to a-aminoadipic acid. We used a two-stage process ("chromosome walking") to screen a lambda library of Streptomyces clavuligerus genomic DNA for fragments that expressed lysine r-aminotransferase activity in S. lividans. Restriction analysis of the cloned DNA confirmed the location of the putative lat gene within the cluster of , -lactam biosynthesis genes, roughly midway between pcbC, the structural gene for isopenicillin N synthetase, and the putative cefE gene encoding deacetoxycephalosporin C synthetase. clavuligerus