Miracle-dz/UniFAR-FTUs huggingface dataset:
Plasmodium falciparum Replication factor C subunit 1 is involved in genotoxic stress response

About half the world’s population is at risk of malaria, with Plasmodium falciparum malaria being responsible for the most malaria related deaths globally. Antimalarial drugs such as chloroquine and artemisinin are directed towards the proliferating intra-erythrocytic stages of the parasite, which is responsible for all the clinical symptoms of the disease. These antimalarial drugs have been reported to function via multiple pathways, one of which induces DNA damage via the generation of free radicals and reactive oxygen species. An urgent need to understand the mechanistic details of drug response and resistance is highlighted by the decreasing clinical efficacy of the front line drug, Artemisinin. The replication factor C subunit 1 protein is an important component of the DNA replication machinery and DNA damage response mechanism. Here we show the translocation of PfRFC1 from an intranuclear localization to the nuclear periphery indicating an orchestrated progression of distinct patterns of replication in the developing parasites. PfRFC1 responds to genotoxic stress via elevated protein levels in soluble and chromatin bound fractions. Reduction of PfRFC1 protein levels upon treatment with antimalarials suggests an interplay of replication and DNA repair pathways leading to cell death. Additionally, mislocalization of the endogenously tagged protein confirmed its essential role in parasites’ replication and DNA repair. This study provides key insights into DNA replication, DNA damage response and cell death in plasmodium falciparum. Importance Frontline drugs have been found to induce DNA damage in the human malaria parasite Plasmodium falciparum. The genotoxic stress response in Plasmodium and the interplay between DNA damage repair, replication and activation of programmed cell death pathways remains largely undescribed. This study shows a distinct pattern of localization of PfRFC1 during replication and DNA repair. PfRFC1 responds to genotoxic stress with an increase in protein expression. Interfering with the RFC complex formation or mislocalization of PfRFC1 is associated with disrupted genotoxic stress response. Additionally, a reduction of PfRFC1 protein levels is observed upon treatment with antimalarial drugs or under apoptosis like conditions, highlighting the role of DEVD/G like motif in mediating programmed cell death in these parasites. This study sheds light on the role of PfRFC1 in differentially responding to replication, genotoxic stress and programmed cell death in Plasmodium parasites.
Fitness Level Influences White Matter Microstructure in Postmenopausal Women

Aerobic exercise has both neuroprotective and neurorehabilitative benefits. However, the underlying mechanisms are not fully understood and need to be investigated, especially in postmenopausal women, who are at increased risk of age-related disorders such as Alzheimer’s disease and stroke. To advance our understanding of the potential neurological benefits of aerobic exercise in aging women, we examined anatomical and functional responses that may differentiate women of varying cardiorespiratory fitness using neuroimaging and neurophysiology. A total of 35 healthy postmenopausal women were recruited (59 ± 3 years) and cardiorespiratory fitness estimated (22–70 mL/kg/min). Transcranial magnetic stimulation was used to assess -aminobutyric acid (GABA) and glutamate (Glu) receptor function in the primary motor cortex (M1), and magnetic resonance spectroscopy (MRS) was used to quantify GABA and Glu concentrations in M1. Magnetic resonance imaging was used to assess mean cortical thickness (MCT) of sensorimotor and frontal regions, while the microstructure of sensorimotor and other white matter tracts was evaluated through diffusion tensor imaging. Regression analysis revealed that higher fitness levels were associated with improved microstructure in pre-motor and sensory tracts, and the hippocampal cingulum. Fitness level was not associated with MCT, MRS, or neurophysiology measures. These data indicate that, in postmenopausal women, higher cardiorespiratory fitness is linked with preserved selective white matter microstructure, particularly in areas that influence sensorimotor control and memory.
Ectosymbionts of the non‐indigenous Asian shore crab, Hemigrapsus sanguineus (Decapoda: Varunidae), in the western north Atlantic, and a search for its parasites

Over 560 Asian shore crabs, Hemigrapsus sanguineus, collected mainly in the spring of 2005 and 2006 from rocky intertidal locations in southern New Jersey, were examined for epibionts. Small numbers of the sympatric green crab, Carcinus maenas, and the Atlantic mud crab, Panopeus herbstii, were examined for epifauna to compare with H. sanguineus. Blue mussel spat, Mytilus edulis, and the encrusting, cheilostome bryozoan, Conopeum tenuissimum, were the dominant ectosymbionts of the shore crab, with prevalences of 22.2 and 32.1%, respectively; ranges of intensity were 1–146 spat/crab and 1–31 colonies/crab. Both species are incidental symbionts. Larger crabs had higher prevalences and intensities of C. tenuissimum colonies, and these colonies displayed a distinct pattern of attachment to the carapace which seemed to be related to crab morphology and habitat. Much less common was the encrusting, ctenostome bryozoan Alcyonidium albescens, a facultative symbiont species with a prevalence of 3.4%. Other epibionts were the encrusting, cheilostome Membranipora tenuis, the tubicolous polychaetes Hydroides dianthus, Sabellaria vulgaris, and Spirorbis sp. , the barnacles Balanus improvisus and Semibalanus balanoides, and unidentified thecate hydroids, all incidental symbionts with prevalences from 0.2 to 3.2%. The total number of known ectosymbionts of H. sanguineus, including additional species found previously in the USA and the western Pacific, is 13. Carcinus maenas and P. herbstii share some of the same symbionts. Rhizocephalan externae were not observed in any of the crab species used in this study, nor were gill parasites or internal parasites found among 248 specimens of H. sanguineus.
BinDex: A Two-Layered Index for Fast and Robust Scans

In modern analytical database systems, the performance of the data scan operation is of key importance to the performance of query execution. Existing approaches may be categorized into index scan and sequential scan. However, both approaches have inherent inefficiencies. Indeed, sequential scan may need to access a large amount of unneeded data, especially for queries with low selectivity. Instead, index scan may involve a large number of expensive random memory accesses when the query selectivity is high. Moreover, with the growing complexities in database query workloads, it has become hard to predict which approach is better for a particular query. In order to obtain fast and robust scans under all selectivities, this paper proposes BinDex, a two-layered index structure based on binned bitmaps that can be used to significantly accelerate the scan operations for in-memory column stores. The first layer of BinDex consists of a set of binned bitmaps which filter out most unneeded values in a column. The second layer provides some auxiliary information to correct the bits that have incorrect values. By varying the number of bit vectors in the first layer, BinDex can make a tradeoff between memory space and performance. Experimental results show that BinDex outperforms the state-of-the-art approaches with less memory than a B+-tree would use. And by enlarging the memory space, BinDex can achieve up to 2.9 times higher performance, eliminating the need for making a choice between sequential or index scans.
MÉTODOS DE AVALIAÇÃO DA ESTABILIDADE OXIDATIVA DE ÓLEOS E GORDURAS

Apresenta revisao de literatura sobre metodos de determinacao da estabilidade oxidativa que surgiram numa tentativa de se avaliar a resistencia a oxidacao dos oleos e gorduras. Existe, atualmente, muita controversia sobre a eficiencia ou a capacidade destes metodos de predizerem as alteracoes que ocorrerao nos oleos e gorduras, durante o armazenamento. Foram discutidas as vantagens e desvantagens de cada metodo e a sua importância na area de tecnologia de oleos e gorduras. A padronizacao das metodologias para determinacao da estabilidade oxidativa, seja pelo metodo de estufa ou pelos metodos automatizados, e uma necessidade em funcao da grande quantidade de dados relatados na literatura que foram obtidos em condicoes muito diversas, dificultando sua utilizacao ate para simples comparacao. OXIDATIVE EVALUATION METHODS OF OILS AND FATS Abstract A literature review on the methods of determination of the oxidative stability originated from an attempt to evaluate the oils and fats oxidation resistance is presented. Nowadays, there is much controversy about the efficiency or the capacity of these methods to predict the alterations that will occur in oils and fats during the storage. It is discussed the advantages and the disadvantages of each methodology and its importance within the area of the oils and fats technology. The standardization of the methodologies for determination of the oxidative stability, by incubators or by automated methods, is a necessity due to the great quantity of data related in literature which were obtained in diverse conditions, making difficult its utilization even for simple comparation.
Divergent lineages in the heath mouse (Pseudomys shortridgei) are indicative of major contraction to geographically isolated refugia on the eastern and western sides of Australia during the early Pleistocene

The heath mouse (Pseudomys shortridgei) is a locally rare species; it was considered extinct in Western Australia until its rediscovery 20 years ago. It is not often detected in faunal surveys and is confined to two ecologically distinct habitats on either side of the Australian continent. An important and immediate conservation question has been to determine the genetic diversity within each of its current habitats and to determine the differences between the geographically separate populations. Measures of genetic differentiation amongst P. shortridgei populations in their eastern (Victoria) and western (Western Australia) distribution were estimated using nuclear (microsatellite loci) and partial sequence of mitochondrial DNA (427bp Cytochrome b gene and 637bp of the D-loop). Both markers identified differences between the east- and west-coast populations. MtDNA data showed significant divergence between populations with monophyly within them, and nuclear loci investigated also showed two clear genetic clusters based on Bayesian inference. As a result of these findings, we suggest that the heath mouse comprises two highly divergent (but genetically diverse) lineages and the aridity of the Nullarbor Plain has clearly been a barrier for dispersal since the early Pleistocene (∼1.43million years ago). The populations either side of the Nullarbor Plain are genetically differentiated and should be defined as separate Evolutionary Significant Units (ESUs).
Enhanced Signaling Through the TLR9 Pathway Is Associated With Resistance to HIV-1 Infection in Chinese HIV-1–Exposed Seronegative Individuals

Innate immunity is the first line of defense against invading pathogens and may mediate HIV-1 resistance in HIV-1–exposed seronegative (HESN) individuals. This study aims to identify components of innate immunity that confer natural HIV-1 resistance in Chinese HESN individuals. Specifically, we compared the expression levels of Toll-like receptors (TLRs) and associated pathway molecules in peripheral blood mononuclear cells (PBMCs), monocytes/macrophages, and plasma obtained from HESN and control individuals. HESN individuals had higher expression of TLR9, IRF7, IFN-α/β, RANTES, and MIP-1α/1β in PBMCs and plasma than control subjects. Upon TLR9 stimulation, significantly higher expression of TLR9 and IRF7, as well as higher production of IFN-α/β, RANTES, and MIP-1α/1β, was observed in PBMCs and monocytes/macrophages from HESN individuals than in the corresponding cells from control individuals. More importantly, both with and without TLR9 stimulation, the levels of HIV-1 replication in monocyte-derived macrophages (MDMs) from HESN individuals were significantly lower than those in MDMs from control individuals. These data suggest that increased TLR9 activity and subsequent release of antiviral factors contribute to protection against HIV-1 in HESN individuals.
Whole-Body Radioprotective Effects of SBL-1: A Preparation From Leaves of Hippophae rhamnoides

The radioprotective effects of Hippophae rhamnoides (common name, sea buckthorn) leaf extract, designated SBL-1, were investigated in Swiss Albino strain ‘A’ mice. Against 100% mortality in whole-body irradiated (60Co-gamma-rays, 10 Gy) controls, a single dose of the SBL-1 rendered >90% survivors when administered 30 min before irradiation and 90% to 80% survivors when administered 1 to 4 h before irradiation. SBL-1 activated proliferation of hemopoietic stem cells countered a radiation-induced decrease in total thiols and an increase in free radicals in plasma and liver; inhibited lipid peroxidation, and normalized the liver alkaline phosphatase activity. This study demonstrated high radioprotective potential of H. rhamnoides leaves.
Lynch Syndrome Germline Mutations in Breast Cancer: Next Generation Sequencing Case-Control Study of 1,263 Participants

Genome instability—the increased tendency of acquiring mutations in the genome and ability of a cell to tolerate high mutation burden—is one of the drivers of cancer. Genome instability results from many causes including defects in DNA repair systems. Previously, it has been shown that germline pathogenic mutations in DNA Mismatch Repair (MMR) pathway cause cancer-predisposing Lynch Syndrome. We proposed that Lynch Syndrome-related germline mutations (LS-mutations) are associated with breast cancer (BC). In this study, we performed Targeted Next-Generation Sequencing of MMR pathway genes MLH1, MSH2, MSH6, EPCAM, and PMS2 in a cohort of 711 patients with hereditary BC, 60 patients with sporadic BC, and 492 healthy donors. Sixty-nine patients (9.7%) with hereditary BC harbored at least one germline mutation in the MMR pathway genes, of them 32 patients (4.5%) harbored mutations in MMR pathway genes which we define as pathogenic or likely pathogenic, and of them 26 patients (3.6%) did not have any pathogenic mutations in DDR pathway genes, compared to two mutations in MMR pathway genes (0.4%) detected in a group of 492 healthy donors [p = 0.00013, OR = 8.9 (CI 95% 2.2–78.4)]. Our study demonstrates that LS-mutations are present in patients with hereditary BC more frequently than in healthy donors, and that there is an association of hereditary BC and mutations c.1321G>A in MLH1, c.260C>G and c.2178G>C in MSH2, c.3217C>T in MSH6, c.1268C>G and c.86G>C in PMS2 genes. This finding provides a rationale for including pathogenic LS-mutations into genetic counseling tests for patients with hereditary BC.
Low Salinity Improves Photosynthetic Performance in Panicum antidotale Under Drought Stress

Salinity and drought are two often simultaneously occurring abiotic stresses that limit the production of food crops worldwide. This study aimed to distinguish between the separate and combined impacts of drought and salinity on the plant response. Panicum antidotale was cultivated in a greenhouse under the following growth conditions: control, 100 mM NaCl (100) and 300 mM NaCl (300) salinity, drought (D; 30% irrigation), and two combinations of salinity and drought (100 + D and 300 + D). The growth response was as follows: 0 ≈ 100 > 100 + D > > D ≈ 300 ≈ 300 + D. Growth correlated directly with photosynthesis. The net photosynthesis, stomatal conductance, intercellular CO2, transpiration, ribulose 1,5-bisphosphate carboxylase (Rubisco), ribulose 1,5-bisphosphate (RuBP) regeneration, and triose phosphate utilization protein (e.g., phosphoenolpyruvate carboxylase) were highest in the control and declined most at 300 + D, while 100 + D performed significantly better as compared to drought. Maximum and actual photosystem II (PSII) efficiencies, along with photochemical quenching during light harvesting, resemble the plant growth and contemporary CO2/H2O gas exchange parameters in the given treatments. Plant improves water use efficiency under salt and drought treatments, which reflects the high water conservation ability of Panicum. Our findings indicate that the combination of low salinity with drought was able to minimize the deleterious effects of drought alone on growth, chlorophyll content, cell integrity, photosynthesis, leaf water potential, and water deficit. This synergetic effect demonstrates the positive role of Na+ and Cl– in carbon assimilation and osmotic adjustment. In contrast, the combination of high salinity and drought enforced the negative response of plants in comparison to single stress, demonstrating the antagonistic impact of water availability and ion toxicity.
Ultrastructure of Sporogenesis in a Moss, Ditrichum pallidum I. Meiotic Prophase

The ultrastructure of meiotic prophase in a moss is described for the first time. Six stages based on light and electron microscopic observations of Ditrichum pallidum are recognized. 1. Premeiotic sporocytes encased in a poly- saccharide layer, which persists through spore formation, are released into the spore chamber by lysis of the archesporial cell walls. 2. Synaptinemal complexes (SCs) form. 3. Nuclei containing well-developed SCs migrate to the sporocyte periphery and assume the acentric "bouquet" appearance. 4. Pachytene nuclei return to a more central position. 5. Nuclei enter a diffuse stage with chromatin relaxed; cytoplasmic furrows produce four lobes in tetrahedral arrangement; mi- crotubules appear and proliferate around the plastid in each of the four lobes and finally ensheath the nucleus. 6. The nuclear membrane is disassociated; chromatin recondenses into chromosomes and kinetochore-microtubule attachments form. Although no novel structures were observed to be associated with spindle forma- tion and orientation, microtubules were first observed in the region of the plastids. Only plastid behavior, i.e., two successive divisions resulting in four plastids po- sitioned in the four lobes just prior to the onset of meiosis, parallels the phenom- enon of precocious cytoplasmic lobing. Because of the complex and precise processes leading to distinctive and often ornate mature spores, the study of sporogenesis has attracted cell and developmental biologists as well as workers interested in the taxonomic and evolutionary significance of spore and pollen morphology. The growing knowledge of spore and pollen development has been expertly surveyed in several recent books (Heslop-Harrison 1971, Stanley & Linskens 1974, Ferguson & Muller 1976). Compared with present knowledge of pollen development and structure, the study of sporogenesis in homosporous plants is still in its infancy. It is surprising that so little attention has been given to such studies in the bryophytes. Their position as primitive land plants of remarkable diversity and uncertain affinities make them an excellent group of organisms from which evidence of early land plant evolution could be sought. Comparative studies using techniques of modern electron microscopy have brought about an increasing awareness of the utility of spore morphology in answering questions of bryophyte phylogeny (Sorsa 1976).
Relaxant Effects of 17-β-Estradiol in Cerebral Arteries through Ca2+ Entry Inhibition

Estrogens account for gender differences in the incidence and outcome of stroke, but it remains unclear to what extent neuroprotective effects of estrogens are because of parenchymal or vascular actions. Because reproductive steroids have vasoactive properties, the authors assessed the effects and mechanisms of action of 17-β-estradiol in rabbit isolated basilar artery. Cumulative doses of 17-β-estradiol (0.3 μmol/L to 0.1 mmol/L) induced concentration-dependent relaxation that was larger in basilar than carotid artery, in male than female basilar artery, and in KCl-precontracted than UTP-precontracted male basilar artery. Endothelium removal did not modify relaxation induced by 17-β-estradiol in basilar artery, whereas relaxation induced by acetylcholine (1 nmol/L to 0.1 mmol/L) was almost abolished. Neither the estrogen receptor antagonist ICI 182,780 (1 μmol/L), nor the protein synthesis inhibitor cycloheximide (1 μmol/L) affected 17-β-estradiol–induced relaxations. Relaxations induced by the K+ channel openers NS1619 and pinacidil in the same concentration range were greater and lower, respectively, when compared with relaxation to 17-β-estradiol, which was not significantly modified by incubation with the K+ channel blockers charybdotoxin (1 nmol/L and 0.1 μmol/L) or glibenclamide (10 nmol/L and 1 μmol/L). Preincubation with 17-β-estradiol (3 to 100 μmol/L) produced concentration-dependent inhibition of CaCl2-induced contraction, with less potency than the Ca2+ entry blocker nicardipine (0.01 to 10 nmol/L). The authors conclude that 17-β-estradiol induces endothelium-independent relaxation of cerebral arteries with tissue and gender selectivity. The relaxant effect is because of inhibition of extracellular Ca2+ influx to vascular smooth muscle, but activation of estrogen receptors, protein synthesis, or K+ efflux are not involved. Relatively high pharmacologic concentrations of 17-β-estradiol causing relaxation preclude acute vascular effects of physiologic circulating levels on the cerebral circulation.
The establishment of an invasive pest mite, Raoiella indica, affects mite abundance and diversity on coconut plants

Abstract The introduction and establishment of an invasive species in a new habitat represents a major threat to biodiversity and ecosystem structure. A recent example of a mite that has become an invasive species is the red palm mite, Raoiella indica Hirst (Acari: Tenuipalpidae). After its introduction in the Americas, this species has considerably expanded the number of hosts and has been reported as a key pest of some of these. In the present study we investigated the possibility of mitefauna alteration on coconut leaflets (abundance and diversity) mediated by the introduction and establishment of R. indica. A survey was conducted over a period of 1 year in two areas of coconut cultivation: one infested and one free of R. indica. The results of the present study suggest that the introduction of R. indica modifies the mitefauna existing in coconut leaflets. Differences were detected in the abundance and diversity of mites at the level of the taxonomic categories (family and species) and at the level of the trophic groups (predators, herbivores and mites with undefined feeding mode). Additionally, in plants infested by the invasive species, a similar pattern was observed between the fluctuation of the phytoseiid mites and other tenuipalpid mites. In these plants, the density of the tenuipalpid mites (including R. indica) was also the variable that most contributed to explain the fluctuation of phytoseiid mites. This suggests that the invasive species, R. indica, acts as a keystone species, structuring the mitefauna in coconut plants.