Miracle-dz/UniFAR-FTUs huggingface dataset:
Antigen-Specific Regulatory T Cells Cells by Photopheresis Induces Intravenous Infusion of Syngeneic Apoptotic

The basis of extracorporeal photopheresis is the reinfusion of leukocytes previously exposed to 8-methoxypsoralen (8-MOP) and UVA radiation. It has been approved for the palliative treatment of cutaneous T cell lymphoma and has reported beneﬁts in autoimmune diseases, transplant rejection, and graft-vs-host disease. However, the underlying mechanism of photopheresis re-mains unresolved. Because UVB radiation can cause immune tolerance via induction of regulatory T cells, we studied whether photopheresis exerts a similar effect extracorporeally. Therefore, we established a model of photopheresis using a murine model of contact hypersensitivity. Splenocytes and lymph node cells of mice that were sensitized with dinitroﬂuorobenzene were exposed to 8-MOP plus UVA in vitro. Intravenous injection of these cells into naive mice caused inhibition of a hapten immune response, which was lost upon depletion of CD11c (cid:1) cells but not T cells. Mice that received untreated cells or cells exposed to UVA or 8-MOP alone were not affected. Inhibition was cell-mediated and Ag-speciﬁc as demonstrated by transfer of tolerance from the primary recipients into naive animals, which could, however, properly respond to the unrelated hapten oxazolone. Transfer activity was lost when cells were depleted of CD4 (cid:1) or CD25 (cid:1) subpopulations. These data suggest that photopheresis exerts its immunomodu-latory effects via the induction of Ag-speciﬁc regulatory T cells. The Journal of Immunology, 2005, 174: 5968–5976.
The differences in self-reported use and perception of the L1 and L2 of maximally proficient bi- and multilinguals: a quantitative and qualitative investigation

This study investigates language preferences and perceptions in the use of the native language (L1) and second language (L2) by 386 biand multilingual adults. Participants declared that they were maximally proficient in L1 and L2 and used both constantly. A quantitative analysis revealed that despite their maximal proficiency in the L1 and L2, participants preferred to use the L1 for communicating feelings or anger, swearing, addressing their children, performing mental calculations, and using inner speech. They also perceived their L1 to be emotionally stronger than their L2 and reported lower levels of communicative anxiety in their L1. An analysis of interview data from 20 participants confirmed these findings while adding nuance. Indeed, differences in the use of the L1 and L2 and perceptions of both are often subtle and contextspecific. Participants confirmed the finding that the L1 is usually felt to be more powerful than the L2, but this did not automatically translate into a preference for the L1. Longer stretches of time in the L2 culture are linked to a gradual shift in linguistic practices and perceptions. Participants reported that their multilingualism and multiculturalism gave them a sense of empowerment and a feeling of freedom.
Consiglio Nazionale delle Ricerche Towards a Timely Prediction of Earthquake Intensity

A growing number of people is turning to Social Media in the aftermath of emergencies to search and publish critical and up to date information. Retrieval and exploitation of such information may prove crucial to decision makers in order to minimize the impact of disasters on the population and the infrastructures. Yet, to date, the task of the automatic assessment of the consequences of disasters has received little to no attention. Our work aims to bridge this gap, merging the theory behind statistical learning and predictive models with the data behind social media. Here we investigate the exploitation of Twitter data for the improvement of earthquake emergency management. We adopt a set of predictive linear models and evaluate their ability to map the intensity of worldwide earthquakes. The models build on a dataset of almost 5 million tweets and more than 7,000 globally distributed earthquakes. We run and discuss diagnostic tests and simulations on generated models to assess their signiﬁcance and avoid overﬁtting. Finally we deal with the interpretation of the relations uncovered by the linear models and we conclude by illustrating how ﬁndings reported in this work can be leveraged by existing emergency management systems. Overall results show the eﬀectiveness of the proposed techniques and allow to obtain an estimation of the earthquake intensity far earlier than conventional methods do. The employment of the proposed solutions can help understand scenarios where damage actually occurred in order to deﬁne where to concentrate the rescue teams and organize a prompt emergency response
Proteomic Analysis of Seed Dormancy in Arabidopsis

The mechanisms controlling seed dormancy in Arabidopsis thaliana have been characterized by proteomics using the dormant accession Cvi originating from the Cape Verde Islands. Comparative studies carried out with freshly harvested dormant (D) and after-ripened non-dormant (ND) seeds revealed a specific differential accumulation of 32 proteins. The data suggested that proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry state leading to dormancy release. Exogenous application of ABA to ND seeds strongly impeded their germination, which physiologically mimicked the behavior of D imbibed seeds. This application resulted in an alteration of the accumulation pattern of 71 proteins. There was a strong down-accumulation of a major part (90%) of these proteins, which were involved mainly in energetic and protein metabolisms. This feature suggested that exogenous ABA triggers proteolytic mechanisms in imbibed seeds. An analysis of de novo protein synthesis by two-dimensional (2D) gel electrophoresis in the presence of [ 35 S]-Met disclosed that exogenous ABA does not impede protein biosynthesis during imbibition. Furthermore, imbibed D seeds proved competent for de novo protein synthesis, demonstrating that impediment of protein translation was not the cause of the observed block of seed germination. However, the 2D protein profiles were markedly different from those obtained with the ND seeds imbibed in ABA. Altogether, the data showed that the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal medium.
Research Online Research Online Rumination, realignment and reflection: Who is really teaching Rumination, realignment and reflection: Who is really teaching health education in secondary schools? health education in secondary schools?

In Western Australia (WA), health education (HE) is timetabled as a separate, discipline-based subject belonging to the health and physical education (HPE) learning area. Globally, this subject is identified as a key site to support and strengthen the health and wellbeing of children and young people. In WA, teachers from outside-of-the-field of HPE are commonly used to deliver HE. This situation is not exclusive to HE nor to WA, and in some circles is referred to as education’s dirty little secret. This mixed-methods study critically examined the role of the teacher in the delivery of HE with the purpose of continuing previous WA-based research and responding to a gap in data with regard to the qualifications of the teachers. The study obtained questionnaire responses from 75 teachers delivering HE in 49 different lower secondary government schools across the state with nine teachers interviewed after the first round of quantitative data collection to provide contexualised information of the WA setting. The study found that half of the teachers delivering HE were unprepared to do so.
Increased Expression of Placenta Growth Factor in Chronic Obstructive Pulmonary Disease

Background : Vascular endothelial growth factor (VEGF) and its receptor may play an important role in the pathogenesis of emphysema. But the effect of another angiogenic factor, placenta growth factor (PlGF), to chronic obstructive pulmonary disease (COPD) is unknown. Methods : We measured the levels of VEGF and PlGF in serum from patients with COPD (n=184), smokers (n=212), nonsmokers (n=159), and in bronchoalveolar lavage (BAL) fluid from another group (COPD n=20, controls n=18). In vitro cell culture experiments were performed to investigate the effect of PlGF on VEGF. Results: The serum levels of PlGF were significantly higher in COPD than in controls (27.1 (SE, 7.4) pg/ml vs. 12.3 (SE, 5.1) pg/ml in smokers and 10.8 (SE, 6.3) pg/ml in nonsmokers, p=0.005). The levels of PlGF in BAL fluids were also significantly higher in COPD than in controls (45.7 (SE, 12.3) pg/ml vs. 23.9 (SE, 7.6) pg/ml, p =0.005), associated with an increase of all measured cytokines, like tumor necrosis factor-α (TNF-α ) and interleukin-8 (IL-8). In COPD patients, the levels of PlGF correlated inversely with forced expiratory volume in one second (FEV 1 ) (in serum, r =-0.59, p =0.002; and in BAL fluids, r =-0.51, p=0.001). While the levels of VEGF in serum were the same between COPD and controls, the levels in BAL fluids were significantly lower in COPD than in controls (127.5 (SE, 30.1) pg/ml vs. 237.8 (SE, 36.1) pg/ml, p =0.002). In cultured bronchial epithelial cells, proinflammatory cytokines induced an increase in the protein expression of both PlGF and VEGF. Continuous concomitant treatment with PlGF, TNF-α and IL-8 stimulation reduced VEGF expression and induced cell death. This phenomenon was suppressed by VEGF receptor inhibitor (CBO-P11). Conclusions : PlGF was increased in serum and BAL fluids of COPD patients, and correlated inversely with FEV 1 . Concomitant treatment with PlGF, TNF- α and IL-8 generate detrimental effects on airway epithelial cells. These data suggest that bronchial epithelial cells can express PlGF which may contribute to the pathogenesis of COPD.
University of Birmingham A prospective study of participation in optional school physical education based on self-determination theory

This study examined whether contextual and personal motivational variables, taken from self-determination theory, could predict student cognitive and affective experiences in school physical education (PE), as well as participation in optional PE in the following school year. Structural equation modeling analysis with a sample of 302 British adolescents showed that need support provided by the PE teachers was related to student need satisfaction, which in turn predicted self-determined motivation. The latter predicted directly various motivational indices and indirectly future participation in optional PE. Furthermore, MANOVA tests showed that those who opted for PE ( n = 171), compared to those who did not ( n = 131), reported more positive motivational experiences in the previous school year. The findings call for the promotion of self-determined motivation in PE in order to enhance student positive experiences and participation rates.
Molecular cloning , characterization and expression of immunoglobulin D ( IgD ) on pathogen challenge and PAMPs stimulation in freshwater carp , Catla catla Short running title : Cloning of IgD in the Indian Major Carps

1Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela769008, Odisha, India 2Fish Health Management Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar-751002, Odisha, India *Corresponding author: Dr. Surajit Das, Phone: +91 6612462684; Fax: +91 661 2462022, E-mail: surajit@nitrkl.ac. in or surajit@myself.com
Prognostic value of individual miRNAs for disease-free survival, breast cancer survival and overall survival

model and used a lasso method for variable selection within the Cox regression (38). The tuning parameter λ was chosen by 10-fold cross-validation to minimize model deviance. In summary, given the moderately large set of miRNAs, we used two complementary methodological approaches for selecting miRNAs for further multivariate analysis. The first method used external validation, and only those miRNAs that were also prognostic in TCGA samples, were evaluated for their incremental prognostic value, after adjusting for clinical features and PAM50 subtypes. The second approach used penalized regression (lasso) with all variables included in the model and used cross-validation for feature selection, thus implementing stringent variable selection within the WHEL cohort. Furthermore, the penalized approach examines all predictors simultaneously and could potentially discover new features.
Title: Primary Dysmenorrhea: Associated Symptoms, Impact and Management among Females in the Menoua Division of the West Region of Cameroon. Cross-sectional Study Running Title: Primary Dysmenorrhea in the Menoua Division

Primary dysmenorrhea, or painful menstruation in the absence of pelvic pathology is a common and often debilitating gynecological condition that affects between 45% and 95% of women at reproductive age. ------------------------------------------------------------------------* The current study aimed to assess the prevalence of dysmenorrhea and associated menstrual symptoms and their self-management techniques among females. This cross-sectional study was conducted in the Menoua Division, which has six subdivisions namely: Fokoue, Dschang, NKongni, Penka Michel, Santchou and Fongo tongo . For this purpose, 668 women aged from 12 to 38 years were considered. A self-administered questionnaire was used to collect data. The main results revealed that dysmenorrhea was reported on almost two-thirds of the women (66.17%). In the 442 girls who reported dysmenorrhea Severe pain was reported by 20.71 % of respondents. More than one-third of the respondents (39.75%) reported using pain relief medications with 23.38 % of herbal medicine and 76.54 % of synthetic medicines. The gastrointestinal problems (diarrhoea and vomiting were the most common symptoms experienced. Less than 19.41% of respondents reported having consulted a doctor for their dysmenorrhea, while most of them consulted friends and family.Dysmenorrhea is a very common problem among university students. A number of symptoms were related to dysmenorrhea. Even though it is common, and may not have a pathological cause, few women seek medical advice. Increasing the awareness might help in relieving the burden of this common health problem. This study was done to determine the prevalence, impact, and management of dysmenorrhea in Menoua division.
Evaluation of Safety Risks in Construction Using Fuzzy Failure Mode and Effect Analysis (FFMEA)

The construction industry is one of the most dangerous environments within which to work. For this reason safety-related risk analysis is one of the most significant tasks that has to be undertaken when managing major construction projects. A combination of fuzzy Logic and Failure Mode and Effects Analysis (FMEA), Fault Tree Analysis (FTA), and Analytical Hierarchy Process-Data Envelopment Analysis (AHP-DEA) is applied to improve the process of managing safety risks. Two different types of large scale construction projects were also considered as case studies. It was found that the risk of falling from a height was the most significant risk for both types of project. Moreover, the factors intensifying the risk of injury from workers falling were found to be ignoring safety and lack of personal protective equipment as well as a lack of appropriate training for construction workers. It was also concluded that the framework is applicable to all construction sites, covers all safety aspects and its results are valid.
Type VI Secretion System in Pseudomonas aeruginosa SECRETION AND MULTIMERIZATION OF VgrG PROTEINS □ S

Pseudomonas aeruginosa is a Gram-negative bacterium caus-ing chronic infections in cystic fibrosis patients. Such infections are associated with an active type VI secretion system (T6SS), whichconsistsofabout15conservedcomponents,includingthe AAA (cid:1) ATPase, ClpV. The T6SS secretes two categories of proteins, VgrG and Hcp. Hcp is structurally similar to a phage tail tube component, whereas VgrG proteins show similarity to the puncturing device at the tip of the phage tube. In P. aeruginosa , three T6SSs are known. The expression of H1-T6SS genes is controlled by the RetS sensor. Here, 10 vgrG genes were identified in the PAO1 genome, among which three are co-regulated with H1-T6SS , namely vgrG1a/b/c . Whereas VgrG1a and VgrG1c were secreted in a ClpV1-dependent manner, secretion of VgrG1b was ClpV1-independent. We show that VgrG1a
The use of two complementary DNA assays, AFLP and MLSA, for epidemic and phylogenetic studies of pectolytic enterobacterial strains with focus on the heterogeneous species Pectobacterium carotovorum

Ampliﬁed fragment length polymorphism (AFLP) markers and multilocus sequence analysis (MLSA) were used to analyse 63 bacterial strains, including 30 soft-rot-causing bacterial strains collected from Syrian potato ﬁelds and 33 reference strains. For the MLSA, additional sequences of 41 strains of Pectobacterium and Dickeya , available from the NCBI GenBank, were included to produce a single alignment of the 104 taxa for the seven concatenated genes ( acnA , gapA , proA , icd , mtlD , mdh and pgi ). The results indicate the need for a revision of the previously classiﬁed strains, as some potato-derived Pectobacterium carotovorum strains were re-identiﬁed as P. wasabiae . The strains that were classiﬁed as P. carotovorum during the analyses demonstrated high heterogeneity and grouped into ﬁve P. carotovorum highly supported clusters (PcI to PcV). The strains rep-resented a wide range of host plants including potatoes, cabbage, avocados, arum lilies, sugar cane and more. Host speciﬁcity was detected in PcV, in which four of the six strains were isolated from monocotyledonous plants. The PcV strains formed a clearly distinct group in all the constructed phylogenetic trees. The number of strains phylogenetically classiﬁed as subspecies ‘ P. c. subsp. brasiliensis ’ in PcIV dramatically increased in size as a result of the characterization of new isolates or re-identiﬁca-tionofprevious P.carotovorum and P.atrosepticum strains.The P.carotovorum strainsfromSyriaweregroupedintoPcI,PcII andPcIV. Thisgroupingindicatesalackofcorrelationbetweenthegeographicaloriginandclassiﬁcationofthesepathogens.
Haematological Indices and Obstetric Outcomes Among Pregnant Women with Preeclampsia at Iringa Regional Tanzania (14)

Preeclampsia is a common problem in pregnancy after 20 weeks of gestation age. This is when the systolic blood pressure is 140 and above and diastolic blood pressure is above 90mmhg with proteinuria. Hematological system is affected and can result into low platelets, low hemoglobin level, high packed cells volume, and reduced Red blood cells, this can result in bad obstetric outcome. This study aimed to assess the hematological indices in Preeclampsia and their maternal outcomes.
Compressive Behaviour of Cassava ( Manihot Esculentar ) Tuber under Static Quasi Compression Loading, as Influenced by Age and Variety

The influence of maturity age of cassava tubers on the mechanical compressive properties of TME 419, TMS 30001 and Peter cassava varieties was investigated in this research. The cassava tubers were harvested at two maturity stages (10 MAP and 15 MAP) from the research farm of the National Center for Agricultural Mechanization (NCAM), Ilorin, Kwara state, Nigeria. The compressive properties (failure force, compressive crushing force, failure energy and crushing energy) were determined using Universal Testing Machine (Testometric model). From the results, the maturity age and cassava variety had significant (P<0.05) effect on the compressive properties of the cassava tubers. The mechanical compressive crushing force and energy required to break the tubers of the three cassava varieties increases with an increase in maturation of the tubers. Likewise, the samples failure force and failure energy increased across the three cassava cultivars as maturity progresses from 10 MAP to 15 MAP. Thus, these compressive parameters must be given serious attention in the design and development of cassava harvesting and peeling machines.
Taxonomic analysis of the conative traits and competitive success of top male and female bowlers

Diferencirani trenažni pristup, uz dobne i spolne specificnosti, razinu sportske izvrsnosti, osim na subjektivnoj procjeni trenera, trebao bi biti osnovan i na objektivnim znanstvenim metodama, kao sto je klaster analiza odabranih antropoloskih obilježja sportasa. U ovom istraživanju, taksonomskom analizom konativnih karakteristika potencijalno bitnih za uspjeh u kuglanju te pokazatelja uspjeha u kuglackom natjecanju, kuglaci i kuglacice, kandidati za reprezentaciju Hrvatske, razvrstani su u dvije skupine. S obzirom da odabrane konativne karakteristike u prethodnim nisu pokazale ocekivanu povezanost s uspjehom u natjecanju, uvidom u specificnosti procjenjivanih obilježja svake od skupina u koju u sportasi razvrstani, trener potencijalno može uspjesnije individualizirati trenažni rad.
ANTI-CHIKUNGUNYA ACTIVITY OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING CARICA PAPAYA LEAVES IN ANIMAL CELL CULTURE MODEL

Objective: The aim of the present study is to synthesized silver nanoparticles (AgNPs) of papaya L. leaves and evaluation of their anti-Chikungunya activity in vitro . Methods: The AgNPs were prepared using C. papaya leaves extract in 1:4 ratio. Synthesized AgNPs were characterized using ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, and scanning electron microscopy. The cytotoxicity of plant NP and 50% tissue culture infective dose of Chikungunya virus (CHIKV) were determined before antiviral assay by the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. After that, the maximum non-toxic dose (MNTD) and ½MNTD were calculated. The absorbance values were detected using a microplate reader at 595 nm. Median tissue culture infective dose (TCID 50 ) dose was calculated using the Reed and Muench method. In vitro antiviral activity was performed to CHIKV using NP MNTD, ½MNTD, and calculated TCID 50 dose of CHIKV. Results: The MNTD and ½MNTD of C. papaya AgNPs were found to be 125 and 62.5 µg/ml, respectively. The MNTD and ½MNTD brought about 39% and 52% of CHIKV inhibition, respectively, when compared to virus control. The infected cell viability increased (14%) when treated with plant AgNPs at ½MNTD. Conclusion: There are no antiviral agents available to treat CHIKV. The medicinal plants and their metabolites are the most important source for the invention and development of new drugs against many types of disease. In view of the rapid expansion of CHIKV at the global level, there is an urgent need to develop newer anti-Chikungunya drugs with unique drugs target.