Miracle-dz/UniFAR-FTUs huggingface dataset:
Cerebrospinal Fluid Shunt Infections: A Multicenter Pediatric Study

Background: Infections complicate 5%–10% of cerebrospinal fluid (CSF) shunts. We aimed to describe the characteristics and contemporary pathogens of shunt infections in children in Canada and the United States. Methods: Descriptive case series at tertiary care hospitals in Canada (N = 8) and the United States (N = 3) of children up to 18 years of age with CSF shunt infections from July 1, 2013, through June 30, 2019. Results: There were 154 children (43% female, median age 2.7 years, 50% premature) with ≥1 CSF shunt infections. Median time between shunt placement and infection was 54 days (interquartile range, 24 days–2.3 years). Common pathogens were coagulase-negative staphylococci (N = 42; 28%), methicillin-susceptible Staphylococcus aureus (N = 24; 16%), methicillin-resistant S. aureus (N = 9; 5.9%), Pseudomonas aeruginosa (N = 9; 5.9%) and other Gram-negative bacilli (N = 14; 9.0%). Significant differences between pathogens were observed, including timing of infection (P = 0.023) and CSF leukocyte count (P = 0.0019); however, differences were not sufficient to reliably predict the causative organism based on the timing of infection or discriminate P. aeruginosa from other pathogens based on clinical features. Empiric antibiotic regimens, which included vancomycin (71%), cefotaxime or ceftriaxone (29%) and antipseudomonal beta-lactams (33%), were discordant with the pathogen isolated in five cases. There was variability between sites in the distribution of pathogens and choice of empiric antibiotics. Nine children died; 4 (44%) deaths were attributed to shunt infection. Conclusions: Staphylococci remain the most common cause of CSF shunt infections, although antibiotic resistant Gram-negative bacilli occur and cannot be reliably predicted based on clinical characteristics.
Population Dynamics of Heliothrips haemorrhoidalis (Thysanoptera:Thripidae) in Strawberry Cultivars in Southern Brazil

Abstract Heliothrips haemorrhoidalis is a polyphagous thrips species that has been reported as a potential pest in strawberry cultivation in Brazil. Since it has only recently emerged as a pest species in production areas, understanding seasonal trends in the species' activity is particularly important for designing management strategies. Therefore, this study aims to investigate the influence of climate factors (temperature and relative humidity) and six strawberry cultivars (‘Albion', ‘Aromas', ‘Camino Real', ‘Monterey', ‘Portola’, and ‘San Andreas’) on the population dynamics of H. haemorrhoidalis during the 2018–2019 and 2019–2020 growing seasons in southern Brazil. The greatest peak in thrips populations in the cultivars assessed occurred between January and March (summer) in both growing seasons. The relationship between the incidence of H. haemorrhoidalis and temperature was observed to be negative during the autumn, spring, and summer months but positive during winter. Relative humidity levels above 70% had a negative influence on the abundance of insects. Populations of H. haemorrhoidalis varied between the cultivars as follows: ‘Aromas' > ‘Monterey' > ‘San Andreas’ > ‘Albion’ > ‘Camino Real’ > ‘Portola’. Cross-sections of strawberry leaves showed that ‘Monterey’ had the highest density of nonglandular trichomes/mm2 (7.26), while ‘Portola’ cultivar, the greatest predominance of glandular trichomes/mm2 (12.67), which may have contributed to the incidence of H. haemorrhoidalis. The demographic information presented on H. haemorrhoidalis in strawberry cultivars provides a starting point to better understand the insect’s population dynamics. These results also indicate that climatic conditions are a limiting factor for the development of H. haemorrhoidalis.
Implementing a Spiritual Care Subject for Holistic Nursing Practice: A Mixed Method Study

Aim: The aims of this sequential explanatory mixed method study were twofold 1. Firstly, to evaluate the implementation of a spiritual care subject in a nursing program. 2. Secondly, to examine undergraduate nursing students’ perceptions of providing spiritual care within their holistic care practice. Background: Studies conducted internationally indicate many nurses feel inadequately prepared to provide holistic care inclusive of spiritual care due to insufficient spiritual care education. Design: Two phase, sequential explanatory mixed method design which comprised of a quantitative study followed by a qualitative study. Methods: The setting was an Australian faith-based university. Participants comprised of undergraduate nursing students who were enrolled in a spiritual care subject. Findings: Two study findings emerged: 1. Participants’ knowledge and practice of spiritual care were transformed by the spiritual care subject, and 2. The spiritual care subject broadened perceptions of spiritual care so participants viewed themselves more equipped to provide spiritual care in their holistic care provision. Conclusion: The spiritual care subject had an affirmative influence on participants’ perceptions of providing spiritual care within holistic care practice. The findings have implications for nurse educators to consider how spiritual care content can be included within undergraduate nursing curricula.
Novel Homo-Bivalent and Polyvalent Compounds Based on Ligustrazine and Heterocyclic Ring as Anticancer Agents

Bivalent and polyvalent inhibitors can be used as antitumor agents. In this experiment, eight ligustrazine dimers and seven ligustrazine tetramers linked by alkane diamine with different lengths of carbon chain lengths were synthesized. After screening their antiproliferation activities against five cancer cell lines, most ligustrazine derivatives showed better cytotoxicity than the ligustrazine monomer. In particular, ligustrazine dimer 8e linked with decane-1,10-diamine exhibited the highest cytotoxicity in FaDu cells with an IC50 (50% inhibiting concentration) value of 1.36 nM. Further mechanism studies suggested that 8e could induce apoptosis of FaDu cells through the depolarization of mitochondrial membrane potential and S-phase cell cycle arrest. Inspired by these results, twenty-seven additional small molecule heterocyclic dimers linked with decane-1,10-diamine and nine cinnamic acid dimers bearing ether chain were synthesized and screened. Most monocyclic and bicyclic aromatic systems showed highly selective anti-proliferation activity to FaDu cells and low toxicity to normal MCF 10A cells. The structure-activity relationship revealed that the two terminal amide bonds and the alkyl linker with a chain length of 8–12 carbon were two important factors to maintain its antitumor activity. In addition, the ADMET calculation predicted that most of the potent compounds had good oral bioavailability.
Cytotoxic granzyme C–expressing ILC1s contribute to antitumor immunity and neonatal autoimmunity

Innate lymphocytes are integral components of the cellular immune system that can coordinate host defense against a multitude of challenges and trigger immunopathology when dysregulated. Natural killer (NK) cells and innate lymphoid cells (ILCs) are innate immune effectors postulated to functionally mirror conventional cytotoxic T lymphocytes and helper T cells, respectively. Here, we showed that the cytolytic molecule granzyme C was expressed in cells with the phenotype of type 1 ILCs (ILC1s) in mouse liver and salivary gland. Cell fate-mapping and transfer studies revealed that granzyme C–expressing innate lymphocytes could be derived from ILC progenitors and did not interconvert with NK cells, ILC2s, or ILC3s. Granzyme C defined a maturation state of ILC1s. These granzyme C–expressing ILC1s required the transcription factors T-bet and, to a lesser extent, Eomes and support from transforming growth factor–β (TGF-β) signaling for their maintenance in the salivary gland. In a transgenic mouse breast cancer model, depleting ILC1s caused accelerated tumor growth. ILC1s gained granzyme C expression following interleukin-15 (IL-15) stimulation, which enabled perforin-mediated cytotoxicity. Constitutive activation of STAT5, a transcription factor regulated by IL-15, in granzyme C–expressing ILC1s triggered lethal perforin-dependent autoimmunity in neonatal mice. Thus, granzyme C marks a cytotoxic effector state of ILC1s, broadening their function beyond “helper-like” lymphocytes. Description ILC1s express granzyme C, mediate perforin-dependent cytotoxicity, and can contribute to autoimmunity and cancer immunosurveillance. Cytotoxic ILC1s contribute to antitumor immunity and autoimmunity Type 1 innate lymphoid cells (ILC1s) play a key role in various immune responses and are generally thought to provide mostly “helper” function. Here, Nixon et al. used various mouse knockout and fate-mapping models to show that a population of ILC1s expressed the cytotoxic-related molecule granzyme C. These cells were driven by T-bet expression and supported by TGF-β signaling in some organs; IL-15 was able to induce granzyme C expression and ILC1 killing capacity. These ILC1s contributed to antitumor immunity in a breast cancer model and to autoimmune pathology in neonatal mice. Thus, there are cytotoxic ILC1s that contribute to various disease states.
The Temple and the Town at Early Bronze Age I Megiddo: Faunal Evidence for the Emergence of Complexity

The Early Bronze Age is considered to be the period when complex and hierarchical societies first developed in the southern Levant. The appearance of specialization and social complexity is manifested through different aspects of the production stages of animal economy. In this paper, we examine faunal assemblages from two interconnected contemporaneous neighboring sites of differing characters in the Jezreel Valley, Israel: Megiddo, a cult site, and Tel Megiddo East, a town site. Both assemblages are dated to the Early Bronze Age IB (EB IB; 3090–2950 b.c.e.), at the dawn of urbanization in the Near East. The connection between sites, revealed in previous studies of other aspects, is supported by the analysis of faunal remains that reveals intriguing overlaps and divergences. The results of the current study show that the control of resources by the Great Temple in Megiddo also included access to animals and their products, and that it impacted the animal economy in settlements in its hinterland. The impact of this system demonstrates the Great Temple at the center of a larger regional economic organization in the late EB IB that would presage the urban developments of the EB II–III.
Socio-hydrological approach for farmer adaptability to hydrological changes: a case study in salinity-controlled areas of the Vietnamese Mekong Delta

ABSTRACT The Vietnamese Mekong Delta (VMD) is an example of a complex dynamic socio-hydrological system in which societies and hydrology interact and co-evolve. The dominant engineering approach in the VMD has enhanced the dynamics of society and hydrology. This study looks at the implications of socio-hydrological dynamics in the coastal VMD where saline water is controlled by various infrastructures. In the first phase, key informant interviews and focus group discussions were used to explore socio-hydrological dynamics in the study area. The results show divergence in livelihood strategies inside the freshwater-projected area, shaping a heterogeneous agricultural landscape of fresh- and brackish-water livelihoods manifested by four socio-hydrological groups. Next, the Motivation and Ability (MOTA) framework and household surveys were used to assess the adaptability in the agricultural development pathway advocated by the freshwater policy. The result revealed differentiations among these socio-hydrological groups. The findings suggest other practices may be required to help the area navigate towards future adaptations to socio-hydrological changes.
Risk-benefit perceptions of natural gas export in Oregon

ABSTRACT Public perceptions of fracking and natural gas have received significant scholarly attention recently. However, opinion research is scant with respect to natural gas export (the transport and sale of natural gas from one country to another via pipeline or ship) and its associated economic benefits and environmental risks. Understanding public views on export, particularly in places where export facilities are proposed, is important because the U.S. has rapidly increased its natural gas exports overseas in the wake of the shale revolution. Moreover, such facilities have been targeted by anti-fossil fuel and climate activists as a weak link in the chain of the fossil fuel economy, particularly on the U.S. West Coast where proposals for export terminals have proven unsuccessful. Using a web-based opinion survey in the state of Oregon (n = 500), we evaluate risk and benefit perceptions of natural gas export and the role of socio-demographic, political ideology, and place-based factors in shaping these perceptions. We find that men, conservatives, urban residents, and those who perceive that their community’s economic identity is tied to extractive industries are less likely to perceive risks and more likely to perceive benefits from natural gas export. Respondents with a bachelor’s degree and those who perceive that their community’s economic identity is tied to renewable energy are more likely to perceive risks and less likely to perceive benefits. Whereas much of energy social science is focused on sites of extraction, our results contribute to the growing understanding of the social dimensions of energy export.
Forecasting Migration and Integration Trends Using Digital Demography – A Case Study of Emigration Flows from Croatia to Austria and Germany

Abstract This paper was created as a result of the observed instability of external emigration data from Croatian official data in comparison to data from the statistical offices of the European Union (Eurostat) and Germany (DESTATIS). In this study, the author presents a descriptive analysis of alternative data sources (big data), which could be useful for determining emigration flows from Croatia to Austria and Germany, as well as for estimating and forecasting. The second goal of this paper is to show that this approach can be useful for assessing the degree of cultural assimilation/integration of Croatian emigrants using the tools of Google Trends and Facebook Analytics. To estimate the model, linear regression was used to measure the correlation between the number of searches (x) and the number of moves (y) evidenced by the official statistics.
Long‐term outcome evaluation of medium/high risk acute lymphoblastic leukaemia children treated with or without cranial radiotherapy in the EORTC 58832 randomized study

We investigated the long‐term outcome, the incidence of second neoplasms (SN) and the rate of late adverse effects (LAE) in children with central nervous system (CNS) negative medium/high‐risk de novo acute lymphoblastic leukaemia (ALL), in first complete remission (CR1) at end of late intensification, randomized to receive no cranial radiotherapy (No CRT, n = 92) versus CRT (standard arm, n = 84) in the non‐inferiority EORTC 58832 study (1983–1989). Median follow‐up was 20 years (range 4–32 years). The 25‐year disease‐free survival rate (±SE) was 67·4 ± 4·9% without CRT and 70·2 ± 5·0% with CRT. The 25‐year incidence of isolated (6·5 ± 2·6% vs. 4·8 ± 2·3%) and any CNS relapse {8·7 ± 2·9% vs. 11·9 ± 3·5%; hazard ratio (HR) 0·71 [95% confidence interval (CI) 0·28–1·79]; test of non‐inferiority: P = 0·01} was not increased without CRT. The 25‐year SN incidence in CR1 was 7·9 ± 4·6% vs. 11·0 ± 4·2%. The 25‐year event‐free and overall survival rates were quite similar in both arms [59·5 ± 6·3% vs. 60·5 ± 5·9%, HR 0·94 (95% CI 0·57–1·52), and 78·1 ± 4·3% vs. 78·5 ± 4·5%, HR 1·00 (95% CI 0·53–1·88)]. Omission of CRT was associated with dramatic decrease in CNS and endocrine LAE rates. In conclusion, our data suggest that, with proper systemic and intrathecal CNS prophylaxis, CRT could totally be omitted in CR1 without jeopardizing survival, while decreasing LAE in childhood ALL.
The Impact of an Integrated Care Management Program on Acute Care Use and Outpatient Appointment Attendance Among High‐Risk Patients With Lupus

Patients with systemic lupus erythematosus (SLE) often struggle with high acute care use (emergency department [ED] visits and hospitalizations) and missed appointments. A nurse‐led integrated care management program (iCMP) at our multihospital system coordinates care for patients at high risk for frequent acute care use due to comorbidities, demographics, and prior use patterns. We studied whether iCMP enrollment was associated with decreased acute care use and missed appointment rates among patients with SLE.
DEep learning‐based rapid Spiral Image REconstruction (DESIRE) for high‐resolution spiral first‐pass myocardial perfusion imaging

The objective of the current study was to develop and evaluate a DEep learning‐based rapid Spiral Image REconstruction (DESIRE) technique for high‐resolution spiral first‐pass myocardial perfusion imaging with whole‐heart coverage, to provide fast and accurate image reconstruction for both single‐slice (SS) and simultaneous multislice (SMS) acquisitions. Three‐dimensional U‐Net–based image enhancement architectures were evaluated for high‐resolution spiral perfusion imaging at 3 T. The SS and SMS MB = 2 networks were trained on SS perfusion images from 156 slices from 20 subjects. Structural similarity index (SSIM), peak signal‐to‐noise ratio (PSNR), and normalized root mean square error (NRMSE) were assessed, and prospective images were blindly graded by two experienced cardiologists (5: excellent; 1: poor). Excellent performance was demonstrated for the proposed technique. For SS, SSIM, PSNR, and NRMSE were 0.977 [0.972, 0.982], 42.113 [40.174, 43.493] dB, and 0.102 [0.080, 0.125], respectively, for the best network. For SMS MB = 2 retrospective data, SSIM, PSNR, and NRMSE were 0.961 [0.950, 0.969], 40.834 [39.619, 42.004] dB, and 0.107 [0.086, 0.133], respectively, for the best network. The image quality scores were 4.5 [4.1, 4.8], 4.5 [4.3, 4.6], 3.5 [3.3, 4], and 3.5 [3.3, 3.8] for SS DESIRE, SS L1‐SPIRiT, MB = 2 DESIRE, and MB = 2 SMS‐slice‐L1‐SPIRiT, respectively, showing no statistically significant difference (p = 1 and p = 1 for SS and SMS, respectively) between L1‐SPIRiT and the proposed DESIRE technique. The network inference time was ~100 ms per dynamic perfusion series with DESIRE, while the reconstruction time of L1‐SPIRiT with GPU acceleration was ~ 30 min. It was concluded that DESIRE enabled fast and high‐quality image reconstruction for both SS and SMS MB = 2 whole‐heart high‐resolution spiral perfusion imaging.
Comprehensive Analysis of Tumor Immune Microenvironment Characteristics for the Prognostic Prediction and Immunotherapy of Oral Squamous Cell Carcinoma

Background: Oral squamous cell carcinoma (OSCC) is the most common cancer of oral and maxillofacial region. A recent clinical research has shown that tumor immune microenvironment (TIME)cells are closely related to immunotherapy sensitivity and OSCC prognosis. Nonetheless, a comprehensive analysis of TIME in OSCC has not been reported. Methods: Bioinformatics and computational algorithms were employed to determine the significance of TIME cells in 257 OSCC patients. TIME scores were measured by three TIME models, and then used to evaluate the prognosis of OSCC patients. Results: High TIME score was characterized by better prognosis in OSCC patients less than 60 years old, overexpression of immunotherapy targets (e.g., PD-1 and CLTA-4), and higher T-cell activity to inhibit tumor growth. Besides, poor prognosis was associated with low time score. Conclusion: TIME score exhibited potential as a prognostic biomarker and an indicator in predict immunotherapeutic outcomes. Through the understanding of TIME model, this study can provide a better scheme for immunotherapy as the effective treatment of OSCC patients in the future.
Identification of a Rare Variant of c.1777G>A (p.G593S) in the COL1A1 Gene as the Etiology of Recurrent Osteogenesis Imperfecta by Whole-Exome Sequencing

Background Osteogenesis imperfecta (OI) is a rare heterogeneous disorder typically featured by fragile bones and susceptibility to fracture. The aim of the present study was to explore the genetic etiology of familial recurrent OI and the genotype–phenotype correlation. Methods Karyotyping, chromosomal microarray analysis, and whole-exome sequencing (WES) were performed to determine the genetic etiology of OI in the enrolled family. Western blotting analysis was carried out using the fetal skin tissue for type I collagen production analysis. Results At the first pregnancy, a c.1777G>A mutation in the COL1A1 gene was detected in the fetus who exhibited skeletal dysplasia. In this second pregnancy, severe fetal skeletal dysplasia was also presented without significant chromosomal abnormality detected by karyotype and chromosomal microarray analysis in the fetus. Further WES results demonstrated a de novo missense mutation of c.1777G>A (p.G593S) in the fetus, which was classified as a pathogenic variant according to the ACMG guidelines. The recurrent mutation in the two fetuses hinted at the possible existence of gonadal mosaicism in the parents, while no mutation in the COL1A1 gene was identified in the DNA from the father's sperm. In addition, Western blot results demonstrated no reduced type I procollagen production in the affected fetus compared with the age-matched controls. Conclusions To the best of our knowledge, this is the first study that identified a rare variant of c.1777G>A in the COL1A1 gene that led to recurrent OI in the Chinese population. Additionally, we believe that this rare variant of c.1777G>A in the COL1A1 gene will lead to OI type II. The results of the present study further verify the application value of WES in identifying fetuses with ultrasound anomalies.
Emerging Roles of Inflammasomes in Cardiovascular Diseases

Cardiovascular diseases are known as the leading cause of morbidity and mortality worldwide. As an innate immune signaling complex, inflammasomes can be activated by various cardiovascular risk factors and regulate the activation of caspase-1 and the production and secretion of proinflammatory cytokines such as IL-1β and IL-18. Accumulating evidence supports that inflammasomes play a pivotal role in the progression of atherosclerosis, myocardial infarction, and heart failure. The best-known inflammasomes are NLRP1, NLRP3, NLRC4, and AIM2 inflammasomes, among which NLRP3 inflammasome is the most widely studied in the immune response and disease development. This review focuses on the activation and regulation mechanism of inflammasomes, the role of inflammasomes in cardiovascular diseases, and the research progress of targeting NLRP3 inflammasome and IL-1β for related disease intervention.