Miracle-dz/UniFAR-FTUs huggingface dataset:
Subcellular localization of Kv10.1 (Eag1): functional ion channels on the inner nuclear membrane

hEag1 (human ether-a-go-go-1, Kv10.1) is a voltage-gated potassium channel expressed mostly in the brain in healthy subjects but also in many tumors under pathological conditions. Its ability to induce malignant transformation and to favour tumor progression can t be attributed entirely to its potassium permeation. hEag1 was shown to co-localize with lamin A/C. And electron microscopy studies in neurons and tumor cells indicated that it is localized at the inner nuclear membrane. The aim of the thesis was to conﬁrm this localization. The peri-nuclear hEag1 fulﬁls the established criteria for transmembrane proteins targeted to the inner nuclear membrane. The peri-nuclear hEag1 was not accessible to antibodies unless the nuclear envelope was permeabilized. Compared to the cytoplasm population, the peri-nuclear population was more resistant to Triton X-100 extraction and less mobile in lateral diﬀusion in FRAP analysis. It was also frequently found in the NPC-free island , which normally associates with the heterochromatin. All these characters suggest a tight interaction with nuclear lamina, other inner nuclear membrane proteins or chromatin. Furthermore, in density gradient centrifugation of extracts from both heterologous and native systems, hEag1 was enriched in fractions containing known inner nuclear membrane proteins. It was also shown that hEag1 at the inner nuclear membrane is functional as an ion channel. Single channel currents detected at the inner nuclear membrane were compatible with that of hEag1 in terms of conductance and pharmacologic properties (inhibition by astemizole and a speciﬁc monoclonal antibody). This current was not detected in non-transfected cells. Moreover, the opening probability was higher when the pipette voltage was more negative, suggesting that hEag1 in the inner nuclear membrane has its N and C termini facing the nucleoplasm. Our experiments strongly suggest that the channel is integrated in the inner nuclear membrane, with its N and C-termini exposed to the nucleoplasm, possibly in contact with the heterochromatin.
Dynamics in groundwater and surface water quality : from field-scale processes to catchment-scale models

In dit proefschrift wordt beschreven hoe innovatieve metingen van afvoer en waterkwaliteit leiden tot de formulering van nieuwe concepten over interacties tussen grondwater en oppervlaktewater op stroomgebiedsschaal. We laten zien dat simpele modellen met weinig parameters in staat zijn nutrienttransport op stroomgebiedschaal nauwkeurig te beschrijven. Deze modellen blijken momenteel het meest effectief in het beschrijven van de geobserveerde korte- en langetermijn dynamiek van waterkwaliteit vanuit perceelschaalprocessen. Deze modellen moeten zich dan wel richten op een correcte beschrijving van de routes en reistijden van water binnen het stroomgebied in plaats van zich alleen te concentreren op een correcte reproductie van de afvoer. In dit proefschrift hebben we op een succesvolle manier modelconcepten ontwikkeld en getest die afvoer beschrijven als een samengestelde afvoer van diverse stroomroutes of als mix van waterdeeltjes met verschillende reistijden. Om deze modellen te kunnen verbeteren en valideren zijn nieuwe vergelijkbare datasets nodig van andere stroomgebieden. Ook kunnen de hier geintroduceerde concepten verder worden ontwikkeld door stroomgebieden met verschillende topografie en klimaten met elkaar te vergelijken. Het doorontwikkelen van de geintegreerde benadering zoals uitgevoerd in dit onderzoek, met een nadruk op een sterkere integratie van de processen die zich afspelen in waterlopen, zullen stroomgebiedschaal modellen opleveren die een meer duurzaam beheer van grondwater en oppervlaktewater mogelijk maken.cum laude graduation (with distinction)
Control of chlorogenic acid formation in leaves and endosperm of coffee fruit of Coffea arabica.

EST sequences of genes coding for enzymes involved in chlorogenic acid (CGA) biosynthesis were identified in the Brazilian Coffee Genome Project databank and used to study the control of the content of this phenol in leaves and fruits of C. arabica cv. Mundo Novo. RTPCR analyses showed reduction in the expression of PAL, C4H, C3H, 4CL and CQT with tissue age. In the fruit endosperm a marked decrease in the final maturation stage was observed. Etiolated seedlings obtained from seeds germinated in the dark and transferred to light showed a significant increase of CGA after 24 h. The increase was transient and paralleled by the expression of PAL, C4H, C3H, 4CL and CQT. Therefore, our results suggest that CGA formation can be manipulated by altering the expression of these genes.
The reciprocal influence of exercise on academic performance and relationships

The current study seeks to better understand exercise habits among the college demographic. It examined the relationships between exercise patterns and aspects of college performance and life. The study explored how exercise influences the lives of college students including relationships, academics and work performance as outlined by Segar (2015). Specifically, the study explored the association between exercise and GPA, as well as the perceived interaction between exercise and academic performance, university and home friendships, and work performance. A survey was completed within a general education course at a midwestern university (81% response rate, N=502). Godin & Shephard’s (1985) validated Leisure Time Exercise Questionnaire was used to measure total exercise scores, and measures of perceived exercise benefits were developed for the study based on Segar (2015). One-way analysis of variance (ANOVA) tests, Pearson bivariate correlations, and linear regression analyses were completed to evaluate differences in student GPA based on exercise habits, and the associations between exercise patterns and perceived benefits of exercise on academic performance, friend and family relationships, and work performance. Female participants with higher GPAs were found to report more exercise as compared to those with lower GPAs. The regression analyses found significant associations between the perception that exercise benefits academic performance and actual exercise patterns, and for female students, between the perception that exercise benefits work performance and actual exercise patterns. The results add to existing research on the relationship between exercise and academics by providing context for the college demographic (including exercise patterns and perceptions differences between male and female students), and by applying Segar’s (2015) model of exercise benefits. Implications suggest strategies universities may implement in order to apply Segar’s (2015) theory to fitness marketing and programming.
Successive production of biodiesel and bioethanol feedstock from the Cosmarium sp

Cosmarium sp is unicellular microalga which was grown in bold basal medium with the controlled condition and investigated biochemical characterizations for biodiesel production and evaluated standardization of ethanol production. Once microalgae were reaches the proper growth stage total lipid was analyzed and these lipids were used for the production of biodiesel by transesterification process using Calcium oxide (0.66%) as a catalyst. Bioethanol production was done by using the defatted residues of Cosmarium sp. with Saccharomyces cerevisiae (10%). In view of the above-mentioned facts, the present study has been designed to enhance the production of biodiesel and bioethanol.
Genotoxicicological Safety Estimate for the Rhus-II

These observations were performed to investigate the safety of the natural herbs (Rhus-II) in respect of genotoxicity. This substance was examined in two in-vitro tests: (1) Salmonella typhimurium reversion assay (Ames test) in strain TA 98, TA 100, TA 1535 and TA 1537, (2) in vitro chromosome aberration test in cultured Chinese hamster ovary (CHO) cells. In the reverse mutation test, Rhus-II did not induced mutagenicity in Salmonella typhimurium reversion assay(Ames test) with or without metabolic activation. In the chromosome aberration assay using CHO cells, there was no increased incidence of structural and numerical aberrations with or without metabolic activation. These results indicated that, the Rhus-II had no genotoxicity.
Who wants to hire a more diverse faculty? A conjoint analysis of faculty and student preferences for gender and racial/ethnic diversity

ABSTRACT What explains the scarcity of women and under-represented minorities among university faculty relative to their share of Ph.D. recipients? Among many potential explanations, we focus on the “demand” side of faculty diversity. Using fully randomized conjoint analysis, we explore patterns of support for, and resistance to, the hiring of faculty candidates from different social groups at two large public universities in the U.S. We find that faculty are strongly supportive of diversity: holding other attributes of (hypothetical) candidates constant, for example, faculty at both universities are between 11 and 21 percentage points more likely to prefer a Hispanic, black, or Native American candidate to a white one. Furthermore, preferences for diversity in faculty hiring are stronger among faculty than among students. These results suggest that the primary reason for the lack of diversity among faculty is not a lack of desire to hire them, but the accumulation of implicit and institutionalized biases, and their related consequences, at later stages in the pipeline.
In vitro investigations on uptake and toxicity of cyanobacterial toxins

s & Poster presentations Fischer, A. , Hoeger, S. J., Feurstein, D. J., Ernst, B. , and Dietrich, D. R. (2007). Importance of organic anion transporting polypeptides (OATPs) for the toxicity of single microcystin congeners in vitro. 7th International Conference on Toxic Cyanobacteria, Rio das Pedras, Rio de Janeiro State, Brazil. Feurstein, D. , Fischer, A. , and Dietrich, D. (2007). In vitro toxicity of microcystins in primary murine whole brain and neuronal cultures. 7th International Conference on Toxic Cyanobacteria, Rio das Pedras, Rio de Janeiro State, Brazil. Feurstein, D. , Fischer, A. , and Dietrich, D. (2008). Microcystin congener-specific in vitro neurotoxicity. Toxicology Letters 180, 103.
A Cross Sectional Study to Ascertain the Ability of the FINDRISC Diabetes Risk Assessment Tool to Detect Pre-Diabetes

Prediabetes is characterized by blood glucose levels that are higher than normal but not high enough to be classed as diabetes, and known to be a high risk state. According to the Ministry of Health, pre-diabetic people are 2-3 times more likely to develop diabetes, which is known to be one of the top 10 deadliest diseases in Malaysia. A new screening tool to screen for diabetes was developed in the form of a questionnaire which provided a cheaper and convenient tool as compared to using laboratory based diagnostic tests, that is the Finnish Diabetes Risk Score (FINDRISC). It is the most valid tool preferred for resource-limited settings by International Diabetes Federation (IDF). However, it was derived and validated for the specific Caucasian population and not the Malaysian population. A cross sectional study was performed among medical undergraduates of Melaka Manipal Medical College (MMMC), Malaysia, with a sample size of 180 participants selected by simple random sampling. Any healthy participant between the age of 18-30, not diagnosed with diabetes mellitus, and students from MMMC were included however those that did not give informed consent, did not complete the questionnaire, and participants that fasted prior to the study were excluded. Data was collected by self-administered questionnaires, filled prior to finger prick for blood glucose. Following multivariate analysis, a positive association between the FINDRISC Score and Waist Circumference, BMI, Physical Activity of less than 30 minutes, first degree relatives with diabetes and previous high glucose. An association was also found between the FINDRISC Score and Waist circumference as well as blood glucose. The sensitivity was 46.67% and specificity 64.55%, with Positive Predictive Value 59.35% and Negative predictive value 74.74%, hence the use of the FINDRISC Diabetes Risk Assessment Score is a moderate to poor screening tool for pre-diabetics, this is attributable to its’ poor sensitivity and high specificity, and a negative predictive value higher than positive predictive value.
The temperature‐determined growing season of a submerged hydrophyte

SUMMARY. Temperature determines the growing season of Utricularia purpurea, a common rootless, carnivorous plant at depths of 0–6.5 m in a dimictic oligotrophic lake (Mirror Lake, New Hampshire, U.S.A.). The dynamics of thermal stratification limit the growing season to 8 weeks at a depth of 6 m compared to 17 weeks at 2 m. Light may limit growth at 6 m during the short growing season, but is not limiting in midsummer at 2 m or 4 m. Plants of equivalent size produce 60–75% less biomass annually at 6 m than at 2 or 4 m. The seasonally maximal biomass (in mid-August) is equivalent to the annual net production as calculated from periodic inventories of new, old and senescent tissue (thus P/B= 1.0). Uptake of nutrient elements (N, P, Ca, Mg, Na, K) is synchronous with biomass production. Nutrients do not become enriched in the overwintering tissue (52% of the maximum biomass). This species is unusually rich in Zn (1000 μg g dry wt−1), relatively rich in N (2.9% dry wt). but quite poor in P (0.084%).
Identifying the Main Drivers in Microbial Diversity for Cabernet Sauvignon Cultivars from Europe to South Africa: Evidence for a Cultivar-Specific Microbial Fingerprint

Microbial diversity in vineyards and in grapes has generated significant scientific interest. From a biotechnological perspective, vineyard and grape biodiversity has been shown to impact soil, vine, and grape health and to determine the fermentation microbiome and the final character of wine. Thus, an understanding of the drivers that are responsible for the differences in vineyard and grape microbiota is required. The impact of soil and climate, as well as of viticultural practices in geographically delimited areas, have been reported. However, the limited scale makes the identification of generally applicable drivers of microbial biodiversity and of specific microbial fingerprints challenging. The comparison and meta-analysis of different datasets is furthermore complicated by differences in sampling and in methodology. Here we present data from a wide-ranging coordinated approach, using standardized sampling and data generation and analysis, involving four countries with different climates and viticultural traditions. The data confirm the existence of a grape core microbial consortium, but also provide evidence for country-specific microbiota and suggest the existence of a cultivar-specific microbial fingerprint for Cabernet Sauvignon grape. This study puts in evidence new insight of the grape microbial community in two continents and the importance of both location and cultivar for the definition of the grape microbiome.
Relationship between Work Ability Index and Fatigue among Iranian Critical Care Nurses

Nurses have always been considered as a unique working group because of the physically and mentally demanding tasks they bear at work. Such a working condition exposes them to a high risk of fatigue, which could influence their work ability. Therefore, this study aims to investigate the relationship between work ability index (WAI) and fatigue of critical care nurses in a military hospital. In this descriptive and crosssectional study, 101 nurses (71 females and 30 males) of critical care units filled out both the Persian version of WAI and nurses’ fatigue questionnaires. The nurses were selected from three general hospitals of Tehran by cluster sampling method. The Pearson correlation test was used for investigating the relationship between two quantitative variables, and all the statistical analyses were performed by SPSS v. 21 software. The nurses’ average age was 24.5 years old (±SD=±3.6), and the mean of work experience was 9 years (±SD=±4.13). The mean work ability of the nurses was 40.01 (±SD=±4.05), indicating ―good work ability‖. Fatigue was found in 46.19% of the participants, and the nurses’ fatigue was mainly of mental type (29.8%). There was a significant negative correlation (-0.57) between the scores of WAI and total fatigue (p < 0.001). The negative correlation between WAI and fatigue shows that WAI and fatigue score could be a good predictor of health and quality of work life. Identifying the influential factors of nurses’ work ability and fatigue would help to improve the work condition of critical care nurses.
Studies on Circo and Polyoma Viruses in Psittacine Birds in Egypt, During 2014-2016

In recent years various psittacine birds have been popular and kept as pet animals in Egypt. Circo and Polyomaviruses and are the main causative agents of Psittisine beak and feather deformities. The main objectives of this study were centered on DNA virus infections in psittacine birds with special refreance to Circovirus and Polyomaviruses. Feather and tissue samples were collected from infected birds and examined using PCR based technology.A total of 50 infected Psittacine birds were examined in which feather and tissue sample were collected and screened for the presence Circo and Polyomaviruses by using PCR technology. PCR was used to amplify C1 gene of Circovirus and T gene of polyomavirus in extracted DNA. The highest prevalence of Circovirus infection in feather samples was shown in red rumped parakeet followed by budgerigar and love birds (41.66, 26.92 and 33.33; respectively). The distribution rate of polyomavirus infection in parakeet, budgerigar and love birds were 23 %, 23.7% and 16.66 % respectively.Co-infection with Polyoma and Circovirus, was detected in, three parakeet, six budgerigars and two love birds with a total percentage of 22%. Apparently healthy psittacine birds can be latently infected with either or both Circo and Polyomavirus without showing any clinical symptoms, and become carriers for and virus shedding disease transmission to other birds. In future studies in Egypt, an attempt to isolate Circo and Polyoma viruses from field cases should be done and the isolates could use for development of bivalent prophylactic vaccine for Egyptian psatticine birds.
Microbial Diversity: Form and Function in Prokaryotes

Author(s): Ogunseitan, O | Abstract: © 2005 by Blackwell Science Ltd. All rights reserved. This book offers the first comprehensive, in-depth treatment of microbial diversity for undergraduate and graduate students. Using a global approach, Microbial Diversity illustrates the impact of microorganisms on ecological and Earth system phenomena. Accompanied by a devoted website with resources for both instructors and students: www.blackwellpublishing. com/ogunseitan Uses key ecological and global phenomena to show the continuity of microbial contribution. Illustrates the importance of microbial diversity for the understanding of global physiochemical and biological processes. Presents analyses of microscopic, culture, molecular, and phylogenetic systematic methods. Shows the relevance of microbial diversity to global environmental problems, such as climate change and ozone depletion. Features numerous illustrations, including over 60 4-color photographs of microbes.
Phenotypic and Genotypic Detection of Virulence Factors of Staphylococcus Aureus Isolated from Meat and Meat Products

A total of 250 random samples of meat and meat products 50 of each meat, luncheon, burger, minced meat and sausagesamples were collected from different butchers in Port-Said Governorate. All samples were subjected to bacteriological examination for isolation and identification of Staph. aureus.The results revealed that the Staph. aureus wasisolated in a percentage of 55 (22%). The prevalence of Staph. aureus isolates in the examined meat, luncheon, burger, minced meat and sausage samples were 14%, 20%, 30% ,34% and 12%, respectively. Coagulase positive Staph. aureus (CPS) was isolated in percentage of 33(60%) from the examined meat, luncheon, burger, minced meat and sausage samples. CPS isolates could be detected in meat, luncheon, burger, minced meat and sausage samples with a percentage of 57.15%, 600%, 60%, 58.82% and 66.66%, respectively. The antibiotic resistance of coagulase positive Staph. aureus using 14 antibacterial drugs by disk diffusion method were resistant to Cephalexin, Chloramphenicol, Cprofloxacin, Clindamycin, Erythromycin, Vancomycin, Trimethoprim + Sulphamethoxazole, Gentamicin and Streptomycin and found with a percentage of 87.88%, 84.85%, 75.76%, 72.73%, 72.73%, 69.70%, 63.64%, 60.60% and 57.58% respectively. The conventional PCR assays were developed with specific primers for the detection of different types of virulence genes in 20 strong CPS strains as protein A in Staphyloccocus (spa), coagulase (coa), haemolysin (hlg) and intacellular adhesion (icaA) while multiplex PCR assay were developed to detect of two types of Staphylococcal enterotoxins (sea and seb). The PCR amplification of the spa gene was 3/4, 2/4, 1/4, 1/4 and 2/4 coagulase positive Staph. aureus at 226 bp from meat, luncheon, burger, minced meat and sausage respectively, While the all 20 confirmed coagulase positive Staph. aureus was examined for coagulase (coa) gene using specific gene were positive at 360 bp. 0/4, 2/4, 0/4, 0/4 and 2/4. Identified coagulase positive Staph. aureus isolates were amplified the diagnostic DNA band successfully at 937 bp for hlg gene from meat, luncheon, burger, minced meat and sausage were 4/4, 3/4, 3/4, 3/4 and 2/4 respectively. Amplification of the icaA gene was 4/4, 3/4, 3/4, 3/4 and 2/4 at 1315 bp from meat, luncheon, burger, minced meat and sausage respectively. Multiplex PCR could successfully amplify the diagnostic DNA bands of 102bp and 164 bp, of genes for staphylococcal enterotoxins sea and seb, respectively. The results showed that 1/4, 1/4, 2/4, 1/4 and 0/4 isolates of Staph. aureus was positive for seb at 164 bp from meat, luncheon, burger, minced meat and sausage respectively. While none of the samples were positive for sea. The public health hazards of this microorganism, as well as improve quality status of meat and meat products were discussed.
Perceived Causes and Incidences of Deviant Behaviour Among Secondary School Students in Kwara State

This paper examined causes and incidences of deviant behaviour among secondary school students in Ilorin-east LGA of Kwara State. The research design adopted in this study was descriptive survey. The population comprised all secondary school students in Ilorin-east Local Government Area of Kwara State. The target population were all SSS II students. 10 secondary schools were sampled, while from each selected school 40 senior secondary school two (SSS II) were randomly selected from each school selected. Simple random sampling technique was used to select 400 students out of 3609 SSS II students in selected schools across the L.G. A (Ilorin East). A questionnaire tagged “Causes and Incidences of Deviant Behaviour Questionnaire” (CIDBQ) was adapted from Moses (2013) was used to collect data from the respondents. The independent t-test and Analysis of Variance were used to test null hypotheses formulated at 0.05 alpha level. Based on the findings of this study, it is recommended that shool administrators should clearly spelt out the rules and regulations of the school to students and punish any devaint behaviour act. This can be done during orientation programmes and on the assembly ground. The educational psychologists and counsellors can enlighten the teachers, parents and the community on the causes of deviant behaviours among adolescents .