Miracle-dz/UniFAR-FTUs huggingface dataset:
Uticaj uslova gajenja na morfološke i hemijske osobine i biološke efekte ekstrakata Arnica montana L.

Arnica montana L. (Asteraceae) je visegodisnja planinska biljka cije se cvetne glavice (Arnicae flos) uglavnom koriste za spoljasnju upotrebu kod modrica i uganuca u fitofarmaceutskim preparatima kao sto su masti, kreme i gelovi. Do pre par godina svetsko tržiste pomenute sirovine je zadovoljavano iskljucivo iz spontanog resursa, ali su biljne populacije, zbog prekomernog sakupljanja, znatno degradirane, zbog cega je u vecini evropskih zemalja stavljena zabrana za njeno dalje sakupljanje iz prirode. Obzirom da tražnja za ovom sirovinom na tržistu ne opada, kultivacija arnike se namece kao jedino održivo resenje zadovoljenja potreba farmaceutske industrije. Buduci da A. montana (narodni naziv – arnika) nije autohtona biljna vrsta za podrucje Srbije, iako se spontano javlja u planinskim oblastima nekih susednih zemalja i Balkanskog poluostrva uopste, cilj ovih istraživanja je bio da se iznađe tehnolosko resenje njivske proizvodnje na osnovu ispitivanja uticaja vremena zasnivanja, primene đubrenja i tipa sadnica. U istraživanju su pored cvetne glavice, kao glavne droge, posmatrani i podzemni organi arnike: rizom i koren, kao sporedne sirovine. Prva faza eksperimentalnog rada obuhvatila je trogodisnji tro-faktorijalni poljski ogled nacina proizvodnje arnike, pri cemu su se kao faktori ispitivali: vreme zasnivanja useva (prolecna i jesenja sadnja), đubrenje (stajnjak i NPK) i nacin zasnivanja useva (iz semena i klonskom propagacijom – deobom bokora). U drugoj fazi eksperimenta, izvrsena je karakterizacija sirovine u smislu njene brze identifikacije, hromatografskim tehnikama identifikovani su prisutni sekundarni metaboliti i određen njihov sadržaj u ispitivanim uzorcima i testirani su bioloski efekti odabranih ekstrakata. Ispitivanja su vrsena na ogledu postavljenom 2008. godine na lokalitetu Kaluđerskih bara (1008 m n.v.), na planini Tara, u krugu rasadnika Nacionalnog Parka „Tara“. Obzirom na to da arnika prvu vegetaciju provodi u fazi rozete sva merenja morfoloskih parametara, prinosa i hemijskih karakterizacija sirovina vrsena su tokom 2009. i 2010. godine. Na ogledu su praceni sledeci parameri: precnik rozete, visina cvetnog izdanka, broj cvetnih izdanaka, broj cvetnih glavica, precnik cvetne glavice, broj sekundarnih rozeta. Određeni su sledeci prinosi: prinos cvetne glavice, prinos rizoma, prinos korena i prinos etarskog ulja (rizoma i korena).
Metacognition facilitates the exploitation of unconscious brain states

Can humans be trained to make strategic use of unconscious representations in their own brains? We investigated how one can derive reward-maximizing choices from latent high-dimensional information represented stochastically in neural activity. In a novel decision-making task, reinforcement learning contingencies were defined in real-time by fMRI multivoxel pattern analysis; optimal action policies thereby depended on multidimensional brain activity that took place below the threshold of consciousness. We found that subjects could solve the task, when their reinforcement learning processes were boosted by implicit metacognition to estimate the relevant brain states. With these results we identified a frontal-striatal mechanism by which the brain can untangle tasks of great dimensionality, and can do so much more flexibly than current artificial intelligence.
Effects of Angiopoietin-Like 3 on Triglyceride Regulation, Glucose Homeostasis, and Diabetes

Angiopoietin-like 3 (ANGPTL3) is a regulator of plasma triglyceride (TRG) levels due to its inhibitory action on the activity of lipoprotein lipase (LPL). ANGPTL3 is proteolytically cleaved by proprotein convertases to generate an active N-terminal domain, which forms a complex with ANGPTL8 orchestrating LPL inhibition. ANGPTL3-4-8 mouse model studies indicate that these three ANGPTL family members play a significant role in partitioning the circulating TRG to specific tissues according to nutritional states. Recent data indicate a positive correlation of ANGPTL3 with plasma glucose, insulin, and homeostatic model assessment of insulin resistance (HOMA-IR) in insulin-resistant states. The aim of this review is to critically present the metabolic effects of ANGPTL3, focusing on the possible mechanisms involved in the dysregulation of carbohydrate homeostasis by this protein. Heterozygous and homozygous carriers of ANGPTL3 loss-of-function mutations have reduced risk for type 2 diabetes mellitus. Suggested mechanisms for the implication of ANGPTL3 in carbohydrate metabolism include the (i) increment of free fatty acids (FFAs) owing to the enhancement of lipolysis in adipose tissue, which can induce peripheral as well as hepatic insulin resistance; (ii) promotion of FFA flux to white adipose tissue during feeding, leading to the attenuation of de novo lipogenesis and decreased glucose uptake and insulin sensitivity; (iii) induction of hypothalamic LPL activity in mice, which is highly expressed throughout the brain and is associated with enhanced brain lipid sensing, reduction of food intake, and inhibition of glucose production (however, the effects of ANGPTL3 on hypothalamic LPL in humans need more clarification); and (iv) upregulation of ANGPTL4 expression (owing to the plasma FFA increase), which possibly enhances insulin resistance due to the selective inhibition of LPL in white adipose tissue leading to ectopic lipid accumulation and insulin resistance. Future trials will reveal if ANGPTL3 inhibition could be considered an alternative therapeutic target for dyslipidemia and dysglycemia.
GDNF Gene Therapy to Repair the Injured Peripheral Nerve

A spinal root avulsion is the most severe proximal peripheral nerve lesion possible. Avulsion of ventral root filaments disconnects spinal motoneurons from their target muscles, resulting in complete paralysis. In patients that undergo brachial plexus nerve repair, axonal regeneration is a slow process. It takes months or even years to bridge the distance from the lesion site to the distal targets located in the forearm. Following ventral root avulsion, without additional pharmacological or surgical treatments, progressive death of motoneurons occurs within 2 weeks (Koliatsos et al., 1994). Reimplantation of the avulsed ventral root or peripheral nerve graft can act as a conduit for regenerating axons and increases motoneuron survival (Chai et al., 2000). However, this beneficial effect is transient. Combined with protracted and poor long-distance axonal regeneration, this results in permanent function loss. To overcome motoneuron death and improve functional recovery, several promising intervention strategies are being developed. Here, we focus on GDNF gene-therapy. We first introduce the experimental ventral root avulsion model and discuss its value as a proxy to study clinical neurotmetic nerve lesions. Second, we discuss our recent studies showing that GDNF gene-therapy is a powerful strategy to promote long-term motoneuron survival and improve function when target muscle reinnervation occurs within a critical post-lesion period. Based upon these observations, we discuss the influence of timing of the intervention, and of the duration, concentration and location of GDNF delivery on functional outcome. Finally, we provide a perspective on future research directions to realize functional recovery using gene therapy.
Biological control of parthenium (Parthenium hysterophorus): the Australian experience

ABSTRACT Parthenium is a Weed of National Significance in Australia. Biological control of parthenium in Australia commenced in 1977 and since then nine insect species and two rust fungi have been introduced and established. Seven of them are widespread, however the time taken for field establishment varied widely between various agents, ranging from one to 15 years. Among them, the stem-galling Epiblema moth, the stem-boring Listronotus weevil, the seed-feeding Smicronyx weevil and the root-feeding Carmenta moth occur in all parthenium-infested areas at high population densities. The leaf-feeding Zygogramma beetle occurs only in central and southern Queensland, and not in northern Queensland. The parthenium summer rust occurs seasonally in central and northern Queensland, while the parthenium winter rust is more widespread in southern Queensland than in central Queensland, but does not occur in north Queensland. The sap-feeding Stobaera planthopper and the leaf-mining Bucculatrix moth established and are widespread, but their damage levels remain very low. The stem-galling Conotrachelus weevil and the stem-boring Platphalonidia moth are believed to be established, but at very low abundance. Biological control has resulted in significant reductions in the abundance and impact of parthenium in Australia. As a result, the area infested with parthenium in central Queensland has declined since the mid-1990s. Due to the absence of many of the effective agents in southern and south-eastern Queensland, agents from central Queensland are being redistributed there. Additionally, based on Australian success, many of these agents have also been introduced into other countries around the world.
Opportunities for Antigen Discovery in Metastatic Breast Cancer

Immune checkpoint inhibitor-based immunotherapy (ICI) of breast cancer is currently efficacious in a fraction of triple negative breast cancers (TNBC) as these cancers generally carry high tumor mutation burden (TMB) and show increased tumor infiltration by CD8+ T cells. However, most estrogen receptor positive breast cancers (ERBC) have low TMB and/or are infiltrated with immunosuppressive regulatory T cells (Tregs) and thus fail to induce a significant anti-tumor immune response. Our understanding of the immune underpinning of the anti-tumor effects of CDK4/6 inhibitor (CDKi) treatment coupled with new knowledge about the mechanisms of tolerance to self-antigens suggests a way forward, specifically via characterizing and exploiting the repertoire of tumor antigens expressed by metastatic ERBC. These treatment-associated tumor antigens (TATA) may include the conventional tumor neoantigens (TNA) encoded by single nucleotide mutations, TNA encoded by tumor specific aberrant RNA transcription, splicing and DNA replication induced frameshift (FS) events as well as the shared tumor antigens. The latter may include the conventional tumor associated antigens (TAA), cancer-testis antigens (CTA) and antigens encoded by the endogenous retroviral (ERV) like sequences and repetitive DNA sequences induced by ET and CDKi treatment. An approach to identifying these antigens is outlined as this will support the development of a multi-antigen-based immunotherapy strategy for improved targeting of metastatic disease with potential for minimal autoimmune toxicity against normal tissues.
Study on Prevalence and Pattern of Self Medication Practices in a Rural Area of Bihar

Background: Self-medication is common in most parts. It leads to adverse effects. The present study was conducted to assess the pattern of self -medication and reasons behind this practice.Subjects and Methods:The present study was community based cross-sectional in nature conducted upon 198 adults residing in the rural field practice area. Sociodemographic profile and details of self-medication were noted.Results:63.1% of the respondents were males. 35.9% of them belonged to the age group of 30-40 years. 32.8% were illiterate and 85.9% were Hindu. A total of 198 respondents practiced self-medication (51.2%). Most common symptom was headache (85.9%). Common cold (63.1%), fever (56.1% and gas (31.8%) were other common complains. Paracetamol (90.0%), pantoprazole (51%), other NSAIDs (42.9%) and cold tablets (36.9%) were other medicines used commonly. 69.2% respondents said that they were practicing it for minor illnesses, 36.9% cited economic reasons while 46% said that consulting a physician needed much time and self-medication was time saving.Conclusion: Prevalence of self-medication is high. Strict drug regulations are needed.
Mismatch Negativity Is Not Always Modulated by Lexicality

One factor that is commonly thought to influence MMN amplitude is lexicality; multiple studies have shown that real-word deviants elicit larger MMNs than pseudoword deviants. Here, however, we report data from two experiments challenging this assumption. In the first experiment (N = 48), real-word deviants did not elicit more negative MMNs than pseudoword deviants; the acoustic difference between standard and deviant was identical across these comparisons. In this experiment, the pseudoword deviant [pʰa˨˩] differed from a real-word [pʰa˧˥] in tone only; therefore, to test the possibility that the lexicality effect is real but is restricted to pseudowords that differ from real words by at least one segment, we ran a second experiment which included different items and participants, and also included a control comparison in which the pseudoword ([tsʰei˨˩]) differs from all real words by at least one segment (there is no existing Mandarin morpheme pronounced [tsʰei] in any tone). In the second experiment (N = 36), both types of pseudowords failed to elicit less negative MMNs than real words. These findings, together with other recent studies failing to show lexicality effects in MMN, challenge the assumption that wordhood reliably influences MMN amplitude.
Overexpression of NtDOG1L-T Improves Heat Stress Tolerance by Modulation of Antioxidant Capability and Defense-, Heat-, and ABA-Related Gene Expression in Tobacco

Drought and heat stresses are two major environmental stress factors that severely threaten crop growth and productivity. Plant delay of germination 1-like (DOG1L) family genes play important roles in various developmental processes and stress responses. In our previous study, a tobacco DOG1L gene (NtDOG1L-T) was found to regulate seedling growth and drought response. Unfortunately, the role of DOG1L genes in heat stress response is yet to be studied. Here, we present data supporting the role of DOG1L genes in heat stress and possible underlying molecular mechanisms. Transcript levels of NtDOG1L-T were rapidly induced by heat or abscisic acid (ABA) treatment. Furthermore, NtDOG1L-T promoter activity was markedly activated by ABA or heat stress, as revealed by histochemical staining in transgenic tobacco seedlings. Overexpression of NtDOG1L-T in transgenic lines improved heat stress tolerance. The NtDOG1L-T-transgenic plants exhibited lower levels of reactive oxygen species (ROS) and lipid peroxidation but higher antioxidant enzyme activities in response to heat stress. Furthermore, transcript abundance of some defense-, heat-, and ABA-responsive marker genes was significantly upregulated, as shown by reverse transcription quantitative PCR (qPCR) in these transgenic plants. In conclusion, NtDOG1L-T positively regulates heat stress tolerance possibly by modulation of antioxidant capability and defense-, heat-, and ABA-related gene expression in tobacco. This study may provide valuable resource for the potential exploitation of DOG1Ls in genetic improvement of heat stress tolerance in crops.
EFFECT OF LEADER MEMBER EXCHANGE, ORGANIZATIONAL CULTURE AND EMPLOYEE ENGAGEMENT ON ORGANIZATIONAL CITIZENSHIP BEHAVIOR (CASE STUDY GEN Y IN PERUM LPPNPI)

DOI:10.31933/DIJEFA Abstract: This study aims to determine the effect of the leader member exchange, organizational culture, employee engagement on organizational citizenship behavior. This type of research is descriptive verification method. The population of all employees of Perum LPPNPI, decision-probability sampling technique with a sample of 200 employees. Data Analysis Structural Equation Modeling (SEM) method Partial Least Square (PLS) with 6.0 WarpPLS program. The results showed that the leader member exchange have a significant effect on employee engagement, organizational culture have a significant effect on employee engagement, employee engagement significantly influence the organizational citizenship behavior, leader member exchange have a significant effect on organizational citizenship behavior, and organizational culture significant effect on organizational citizenship behavior.
Contribution of Noncanonical Antigens to Virulence and Adaptive Immunity in Human Infection with Enterotoxigenic E. coli

Enterotoxigenic Escherichia coli (ETEC) contributes significantly to the substantial burden of infectious diarrhea among children living in low- and middle-income countries. In the absence of a vaccine for ETEC, children succumb to acute dehydration as well as nondiarrheal sequelae related to these infections, including malnutrition. ABSTRACT Enterotoxigenic Escherichia coli (ETEC) contributes significantly to the substantial burden of infectious diarrhea among children living in low- and middle-income countries. In the absence of a vaccine for ETEC, children succumb to acute dehydration as well as nondiarrheal sequelae related to these infections, including malnutrition. The considerable diversity of ETEC genomes has complicated canonical vaccine development approaches defined by a subset of ETEC pathovar-specific antigens known as colonization factors (CFs). To identify additional conserved immunogens unique to this pathovar, we employed an “open-aperture” approach to capture all potential conserved ETEC surface antigens, in which we mined the genomic sequences of 89 ETEC isolates, bioinformatically selected potential surface-exposed pathovar-specific antigens conserved in more than 40% of the genomes (n = 118), and assembled the representative proteins onto microarrays, complemented with known or putative colonization factor subunit molecules (n = 52) and toxin subunits. These arrays were then used to interrogate samples from individuals with acute symptomatic ETEC infections. Surprisingly, in this approach, we found that immune responses were largely constrained to a small number of antigens, including individual colonization factor antigens and EtpA, an extracellular adhesin. In a Bangladeshi cohort of naturally infected children <2 years of age, both EtpA and a second antigen, EatA, elicited significant serologic responses that were associated with protection from symptomatic illness. In addition, children infected with ETEC isolates bearing either etpA or eatA genes were significantly more likely to develop symptomatic disease. These studies support a role for antigens not presently targeted by vaccines (noncanonical) in virulence and the development of adaptive immune responses during ETEC infections. These findings may inform vaccine design efforts to complement existing approaches.
AdaFDR: a Fast, Powerful and Covariate-Adaptive Approach to Multiple Hypothesis Testing

Multiple hypothesis testing is an essential component of modern data science. Its goal is to maximize the number of discoveries while controlling the fraction of false discoveries. In many settings, in addition to the p-value, additional information/covariates for each hypothesis are available. For example, in eQTL studies, each hypothesis tests the correlation between a variant and the expression of a gene. We also have additional covariates such as the location, conservation and chromatin status of the variant, which could inform how likely the association is to be due to noise. However, popular multiple hypothesis testing approaches, such as Benjamini-Hochberg procedure (BH) and independent hypothesis weighting (IHW), either ignore these covariates or assume the covariate to be univariate. We introduce AdaFDR, a fast and flexible method that adaptively learns the optimal p-value threshold from covariates to significantly improve detection power. On eQTL analysis of the GTEx data, AdaFDR discovers 32% and 27% more associations than BH and IHW, respectively, at the same false discovery rate. We prove that AdaFDR controls false discovery proportion, and show that it makes substantially more discoveries while controlling FDR in extensive experiments. AdaFDR is computationally efficient and can process more than 100 million hypotheses within an hour and allows multi-dimensional covariates with both numeric and categorical values. It also provides exploratory plots for the user to interpret how each covariate affects the significance of hypotheses, making it broadly useful across many applications.
Insights into the mitochondrial transcriptome landscapes of two Brassicales plant species, Arabidopsis thaliana (var. Col-0) and Brassica oleracea (var. botrytis)

Mitochondria play key roles in cellular energy metabolism within eukaryotic cells. As relics of endosymbiotic bacteria, most (but not all) mitochondria contain their own genome (mitogenome, mtDNA), as well as intrinsic biosynthetic machinery for making organelle RNAs and proteins. The expression of the mtDNA requires regulated metabolism of its transcriptome by nuclear-encoded factors. Post-transcriptional mtRNA modifications play a central role in the expression of the plant mitogenomes, and hence in organellar biogenesis and plant physiology. Despite extensive investigations, a full map of angiosperm mitochondrial transcriptomes, a prerequisite for the elucidation of the basic RNA biology of mitochondria, has not been reported yet. Using RNA-seq data, RT-PCR and bioinformatics, we sought to explore the gene expression profiles of land plant mitochondria. Here, we present the mitochondrial transcriptomic maps of two key Brassicaceae species, Arabidopsis thaliana (var Col-0) and cauliflower (Brassica oleracea var. botrytis). The revised transcriptome landscapes of Arabidopsis and cauliflower mitogenomes provide with more detail into mtRNA biology and processing in angiosperm mitochondria, and we expect that they would serve as a valuable resource for the plant organellar community. Accession numbers Sequences are available at the Sequence Read Archive (accession no. PRJNA472433), for both Arabidopsis thaliana var. Col-0 mtRNA (SRA no. SRX4110179) and Brassica oleracea var. botrytis mtRNA (SRA no. SRX4110177).