Miracle-dz/UniFAR-FTUs huggingface dataset:
Murine Leukemia Virus Integrase Two-End Integration Events by Moloney Assembly and Catalysis of Concerted

Retroviral integration results in the stable and coordinated insertion of the two termini of the linear viral DNA into the host genome. An in vitro concerted two-end integration reaction catalyzed by the Moloney murine leukemia virus (M-MuLV) integrase (IN) was used to investigate the binding and coordination of the two viral DNA ends. Comparison of the two-end integration and strand transfer assays indicates that zinc is required for efﬁcient concerted integration utilizing plasmid DNA as target. Complementation assays using a pair of non- overlapping integrase domains, consisting of the HHCC domain and the core/C-terminal region, yielded products containing the correct 4-base target site duplication. The efﬁciency of the coordinated two-end integration varied depending on the order of addition of the individual protein and DNA components in the complementation assay. Two-end integration was most efﬁcient when the long terminal repeat (LTR) was premixed with ei- ther the target DNA or the HHCC domain. The preference for two-end integration through preincubation of the HHCC ﬁnger with the viral DNA supports the role of this domain in the recognition and/or positioning of the LTR. During the life cycle of retroviruses, the reverse-transcribed is integrated into the in a process catalyzed by integrase be
Is negative density‐dependent reproduction regulated by density‐induced stress in root voles? Two field experiments

Abstract Density dependence in reproduction plays an important role in stabilizing population dynamics via immediate negative feedback from population density to reproductive output. Although previous studies have shown that negative density‐dependent reproduction is associated with strong spacing behavior and social interaction between individuals, the proximal mechanism for generating negative density‐dependent reproduction remains unclear. In this study, we investigated the effects of density‐induced stress on reproduction in root voles. Enclosed founder populations were established by introducing 6 (low density) and 30 (high density) adults per sex into per enclosure (four enclosures per density in total) during the breeding season from April to July 2012 and from May to August 2015. Fecal corticosterone metabolite (FCM) levels, reproductive traits (recruitment rate and the proportion of reproductively active individuals), and founder population numbers were measured following repeated live trapping in both years. The number of founders was negatively associated with recruitment rates and the proportion of reproductively active individuals, displaying a negative density‐dependent reproduction. FCM level was positively associated with the number of founders. The number of founder females directly affected the proportion of reproductive females, and directly and indirectly through their FCM levels affected the recruitment rate; the effect of the number of male founders on the proportion of reproductive males was mediated by their FCM level. Our results showed that density‐induced stress negatively affected reproductive traits and that density‐induced stress is one ecological factor generating negative density‐dependent reproduction.
The effect of servicescape on place attachment and experience evaluation: the importance of exoticism and authenticity in an ethnic restaurant

Purpose Under the growing preference for ethnic food and an increase in ethnic restaurants, it is crucial to understand the value of restaurants gaining a competitive edge for their customers. Therefore, this study aims to investigate how exposure to the servicescape affects the formation of emotion and behavior. Design/methodology/approach This study is based on online survey results pertaining to the servicescape of ethnic restaurants and diners’ evaluations of their experience. The survey items were developed from previous related literature. For accurate data collection, the survey respondents were limited to consumers who had dined in an ethnic restaurant at least once within the previous six months. Findings The results reveal that substantive and communicative servicescape had a positive influence on both exoticism and authenticity; moreover, exoticism positively affected authenticity. In turn, exoticism and authenticity had a positive influence on place attachment. It also appears that place attachment positively affected experience intensification and extension. Practical implications The findings of this study are expected to contribute to competitive management strategies to expand ethnic restaurants. Based on the results, managerial strategies will be set up, focusing on which aspects should receive attention to ensure the intensification and extension of diners’ experiences at ethnic restaurants. Originality/value There has been very little research on ethnic restaurants in relation to the integrated relationship between servicescape, authenticity, place attachment and postexperience behavior. This study assumes that the overall service experience of customers of ethnic restaurants can be perceived and evaluated based on substantive and communicative servicescape and that such restaurants can benefit by understanding the specific factors that will give them a competitive edge in running their business.
Evaluation for internal consistency in the thermodynamic network involving fluorite, cryolite and villiaumite solubilities and aqueous species at 25°C and 1 bar

Abstract Thermodynamic data are constrained by the interrelated thermodynamic equations in addition to the observational measurements and their uncertainties. The consequence is a network of thermodynamic properties that can be evaluated for their internal consistency. In this study, three fluoride minerals that can cause high fluoride concentrations in groundwaters are evaluated for their solubilities and their internal thermodynamic consistency with calorimetric, isopiestic and electrochemical measurements: fluorite, CaF2, cryolite, Na3AlF6, and villiaumite, NaF. This evaluation involves the three solids and 13 aqueous species, the free ions of Ca2+, Na+, Al3+ and F–, and the hydroxido and fluorido complexes of Al3+, and the CaF+ ion pair. For the fluorite–cryolite–villiaumite–aqueous species network, the number of components is minimal, and the solubility studies are mostly of high quality. Re-evaluations of original data using PHREEQC helps to broaden the quantitative evaluation of thermodynamic properties and to resolve apparent discrepancies. A check on this thermodynamic network shows that through a careful appraisal of the literature, a highly consistent set of values can be derived. The resultant infinite-dilution solubility-product constants at 25°C and 1 bar are: for fluorite solubility, logKsp = –10.57 ± 0.08; for cryolite solubility, logKsp = –33.9 ± 0.2; and for villiaumite solubility, logKsp = –0.4981 ± 0.003.
Repeated and Interrupted Resistance Exercise Induces the Desensitization and Re-Sensitization of mTOR-Related Signaling in Human Skeletal Muscle Fibers

The acute resistance exercise (RE)-induced phosphorylation of mTOR-related signaling proteins in skeletal muscle can be blunted after repeated RE. The time frame in which the phosphorylation (p) of mTORS2448, p70S6kT421/S424, and rpS6S235/236 will be reduced during an RE training period in humans and whether progressive (PR) loading can counteract such a decline has not been described. (1) To enclose the time frame in which pmTORS2448, prpS6S235/236, and pp70S6kT421/S424 are acutely reduced after RE occurs during repeated RE. (2) To test whether PR will prevent that reduction compared to constant loading (CO) and (3) whether 10 days without RE may re-increase blunted signaling. Fourteen healthy males (24 ± 2.8 yrs.; 1.83 ± 0.1 cm; 79.3 ± 8.5 kg) were subjected to RE with either PR (n = 8) or CO (n = 6) loading. Subjects performed RE thrice per week, conducting three sets with 10–12 repetitions on a leg press and leg extension machine. Muscle biopsies were collected at rest (T0), 45 min after the first (T1), seventh (T7), 13th (T13), and 14th (X-T14) RE session. No differences were found between PR and CO for any parameter. Thus, the groups were combined, and the results show the merged values. prpS6S235/236 and pp70s6kT421/S424 were increased at T1, but were already reduced at T7 and up to T13 compared to T1. Ten days without RE re-increased prpS6S235/236 and pp70S6kT421/S424 at X-T14 to a level comparable to that of T1. pmTORS2448 was increased from T1 to X-T14 and did not decline over the training period. Single-fiber immunohistochemistry revealed a reduction in prpS6S235/236 in type I fibers from T1 to T13 and a re-increase at X-T14, which was more augmented in type II fibers at T13 (p < 0.05). The entity of myofibers revealed a high heterogeneity in the level of prpS6S235/236, possibly reflecting individual contraction-induced stress during RE. The type I and II myofiber diameter increased from T0 and T1 to T13 and X-T14 (p < 0.05) prpS6S235/236 and pp70s6kT421/S424 reflect RE-induced states of desensitization and re-sensitization in dependency on frequent loading by RE, but also by its cessation.
Design and Analysis of High-Capacity MIMO System in Line-of-Sight Communication

The phase of the channel matrix elements has a significant impact on channel capacity in a mobile multiple-input multiple-output (MIMO) communication system, notably in line-of-sight (LoS) communication. In this paper, the general expression for the phase of the channel matrix at maximum channel capacity is determined. Moreover, the optimal antenna configuration of the 2 × 2 and 3 × 3 transceiver antenna array is realized for LoS communication, providing methods for n×n optimal antenna placement, which can be used in short-range LoS communication and non-scattering environment communication, such as coupling train communication and inter-satellite communication. Simulation results show that the 2 × 2 rectangular antenna array is more suitable for the communication of coupling trains, while the 3 × 3 circular arc antenna array is more suitable for virtual coupling trains according to antenna configurations. Moreover, the 2 × 2 antenna rectangular configuration proposed in this paper has reached the optimal channel in inter-satellite communication, which lays a foundation for the deployment of communication systems.
Macrotermes termite mounds influence the spatial pattern of tree species in two African rainforest sites, in northern Congo. But were they really forests in the past?

Termite mounds have been poorly studied in tropical rainforest in contrast to those of savannahs where they create fertility hotspots and influence the spatial pattern of vegetation. An inventory of termite mounds and of 15 tree species with a diameter at breast height ≥ 10 cm was carried out in two 800-ha blocks, in two rainforest sites located in northern Congo. We used inhomogeneous and intertype K functions to study the spatial pattern of termite mounds and of tree species around mounds, respectively. We found that mounds in Loundoungou were over-dispersed within a radius of less than 70 m, while those in Mokabi were randomly spaced. Tree species within a 20-m radius around a mound were aggregated towards the mound, e.g. Entandrophragma cylindricum, randomly distributed, or even repulsed by the mound. The specific responses also differed in the two sites. These results suggest that (i) the mounds in Loundoungou were created by savannah termite species 3,000-2,000 years BP during the Late Holocene Rainforest Crisis and (ii) the mounds in Mokabi are characteristic of forest mounds. The impact of termite mounds on the spatial pattern of tree species is thus site-dependent, and these differences might be due to species seed dispersal strategies and to soil calcium concentrations.
Motivation, benefits, and challenges for new photovoltaic material & module developments

In the last decade and longer, photovoltaic module manufacturers have experienced a rapidly growing market along with a dramatic decrease in module prices. Such cost pressures have resulted in a drive to develop and implement new module designs, which either increase performance and/or lifetime of the modules or decrease the cost to produce them. In this paper, the main motivations and benefits but also challenges for material innovations will be discussed. Many of these innovations include the use of new and novel materials in place of more conventional materials or designs. As a result, modules are being produced and sold without a long-term understanding about the performance and reliability of these new materials. This has led to unexpected new failure mechanisms occurring few years after deployment, such as potential induced degradation or backsheet cracking. None of these failure modes have been detected after the back then common single stress tests. New accelerated test approaches are based on a combination or sequence of multiple stressors that better reflect outdoor conditions. That allows for identification of new degradation modes linked to new module materials or module designs.
Fear of Death in Medical Students from a Peruvian University during the COVID-19 Pandemic

Due to close contact with death, medical students may question their own and their patients’ dying process, especially with the impact of the COVID-19 pandemic on the increase in deaths. This situation provokes fear and negative attitudes towards dealing with patients and their environment. This study aimed to assess the level of fear of death and associated factors in medical students at a Peruvian university. A cross-sectional analytical study was conducted during March 2021 in human medicine students from the first to the seventh year. A validated survey including the Collet–Lester fear-of-death scale was applied. Factors associated with the fear-of-death score were evaluated by calculating linear regression coefficients (β). A total of 284 students were included. The median age was 22 years, and 58.1% were female. The mean Collet–Lester scale score was 2.79, and it was higher in the dimensions related to the death of others. Adjusted analysis showed that the score on this scale was lower in students aged 24–40 years compared to 17–21 years (β: −0.25; 95% CI: −0.46 to −0.04) and those who had no religious beliefs (β: −0.29; 95% CI: −0.53 to −0.04). In conclusion, fear of death was lower than reported in other investigations despite the COVID-19 situation, being much lower among older students and those without religious beliefs.
Risk of Adverse Pregnancy Outcomes in Young Women with Thyroid Cancer: A Systematic Review and Meta-Analysis

Simple Summary This meta-analysis of 22 articles investigated whether thyroidectomy or radioactive iodine treatment (RAIT) in patients with differentiated thyroid cancer was associated with an increase in adverse pregnancy outcomes, such as miscarriage, preterm delivery, and congenital malformations. The results of this meta-analysis suggest that thyroid cancer treatment, including RAIT, is not associated with an increased risk of adverse pregnancy outcomes, including miscarriage, preterm labor, and congenital anomalies. Abstract This meta-analysis investigated whether thyroidectomy or radioactive iodine treatment (RAIT) in patients with differentiated thyroid cancer (DTC) was associated with an increase in adverse pregnancy outcomes, such as miscarriage, preterm delivery, and congenital malformations. A total of 22 articles (5 case-control and 17 case series studies) from 1262 studies identified through a literature search in the PubMed and EMBASE databases from inception up to 13 September 2021 were included. In patients with DTC who underwent thyroidectomy, the event rates for miscarriage, preterm labor, and congenital anomalies were 0.07 (95% confidence interval [CI], 0.05–0.11; 17 studies), 0.07 (95% CI, 0.05–0.09; 14 studies), and 0.03 (95% CI, 0.02–0.06; 17 studies), respectively. These results are similar to those previously reported in the general population. The risk of miscarriage or abortion was increased in patients with DTC when compared with controls without DTC (odds ratio [OR], 1.80; 95% CI, 1.28–2.53; I2 = 33%; 3 studies), while the OR values for preterm labor and the presence of congenital anomalies were 1.22 (95% CI, 0.90–1.66; I2 = 62%; five studies) and 0.73 (95% CI, 0.39–1.38; I2 = 0%; two studies) respectively, which showed no statistical significance. A subgroup analysis of patients with DTC according to RAIT revealed that the risk of miscarriage, preterm labor, or congenital anomalies was not increased in the RAIT group when compared with patients without RAIT. The results of this meta-analysis suggest that thyroid cancer treatment, including RAIT, is not associated with an increased risk of adverse pregnancy outcomes, including miscarriage, preterm labor, and congenital anomalies.
Acute Unilateral Hypopyon Uveitis and Secondary Glaucoma in an Adult With Relapsing Acute Lymphoblastic Leukemia

Anterior segment infiltration in acute lymphoblastic leukemia (ALL) presenting as hypopyon uveitis in an adult is rare. We report this case as an uncommon presentation in a patient in remission after chemotherapy for ALL. In addition to the hypopyon, the patient presented with congested eye caused by secondary raised intraocular pressure. There is a need to maintain a high index of clinical suspicion in uveitis cases, as early diagnosis of ocular malignancy can save vision. Atypical unilateral hypopyon, even in adults, can be an indication of relapsing ALL.
“I looked it up and you’ll probably be fine”: cancer survivors’ perceptions of helpful and unhelpful support messages and sources

ABSTRACT This study replicated and extended existing research on cancer survivors’ perceptions of helpful and unhelpful social support messages and sources. Forty-three participants with diverse cancer experiences were interviewed about their perceptions of helpful and unhelpful social support messages and sources. Participants recalled six categories of helpful (i.e., network, emotional, esteem, tangible, informational and unspecified) and five categories of unhelpful (i.e., network, emotional, esteem, tangible, informational) messages/sources received during and after cancer treatment. Helpful and unhelpful messages came from spouses, family members, friends, coworkers, healthcare providers and others. Patterns of meaningful message-source combinations are described. Participants recalled double the amount of helpful support messages than nonsupport messages, offering support for the positivity bias. Participants’ memories of helpful support messages and sources during treatment followed a consistent pattern; however, expectations and experiences of received social support after completion of cancer treatment were inconsistent and contradictory.
Design, Spectral Characteristics, Photostability, and Possibilities for Practical Application of BODIPY FL-Labeled Thioterpenoid

This paper presents the design and a comparative analysis of the structural and solvation factors on the spectral and biological properties of the BODIPY biomarker with a thioterpene fragment. Covalent binding of the thioterpene moiety to the butanoic acid residue of meso-substituted BODIPY was carried out to find out the membranotropic effect of conjugate to erythrocytes, and to assess the possibilities of its practical application in bioimaging. The molecular structure of the conjugate was confirmed via X-ray, UV/vis-, NMR-, and MS-spectra. It was found that dye demonstrates high photostability and high fluorescence quantum yield (to ~100%) at 514–519 nm. In addition, the marker was shown to effectively penetrate the erythrocytes membrane in the absence of erythrotoxicity. The conjugation of BODIPY with thioterpenoid is an excellent way to increase affinity dyes to biostructures, including blood components.
Wind and Turbulence Statistics in the Urban Boundary Layer over a Mountain-Valley System in Granada, Spain

Urban boundary layer characterization is currently a challenging and relevant issue, because of its role in weather and air quality modelling and forecast. In many cities, the effect of complex topography at local scale makes this modelling even more complicated. This is the case of mid-latitude urban areas located in typical basin topographies, which usually present low winds and high turbulence within the atmospheric boundary layer (ABL). This study focuses on the analysis of the first ever measurements of wind with high temporal and vertical resolution throughout the ABL over a medium-sized city surrounded by mountains in southern Spain. These measurements have been gathered with a scanning Doppler lidar system and analyzed using the Halo lidar toolbox processing chain developed at the Finnish Meteorological Institute. We have used the horizontal wind product and the ABL turbulence classification product to carry out a statistical study using a two-year database. The data availability in terms of maximum analyzed altitudes for statistically significant results was limited to around 1000–1500 m above ground level (a.g.l.) due to the decreasing signal intensity with height that also depends on aerosol load. We have analyzed the differences and similarities in the diurnal evolution of the horizontal wind profiles for different seasons and their modelling with Weibull and von Mises probability distributions, finding a general trend of mean daytime wind from the NW with mean speeds around 3–4 m/s at low altitudes and 6–10 m/s at higher altitudes, and weaker mean nocturnal wind from the SE with similar height dependence. The highest speeds were observed during spring, and the lowest during winter. Finally, we studied the turbulent sources at the ABL with temporal (for each hour of the day) and height resolution. The results show a clear convective activity during daytime at altitudes increasing with time, and a significant wind-shear-driven turbulence during night-time.
Application of CFD Numerical Simulation in High Speed Train Design

The aerodynamic performance of high speed train(HST) is related to the propulsive efficiency and has influence on the riding comfortability and security.CFD numerical simulation of HST applications is introduced in this paper: the vent system of passenger trains by Airpak;water swaying in water drums of passenger trains,outer flow fields of high speed trains and aerodynamic noises generated by the flow fluid around the 2d streamline HST model by Fluent.The computational result of the vent system agrees with the experimental result.The buckling analysis from I-DEAS integrating Fluent agrees with the failure phenomenon of the water drum.
Percepções de profissionais de enfermagem sobre suas condições de trabalho e saúde no contexto da pandemia de COVID-19

Resumo Objetivo: identificar as condições de trabalho e seus reflexos na saúde de profissionais de enfermagem durante a pandemia de COVID-19, a partir das percepções dos próprios trabalhadores. Métodos: pesquisa qualitativa desenvolvida com 15 profissionais de enfermagem entrevistados por meio de grupos focais online. O conteúdo foi analisado a partir da perspectiva da hermenêutica-dialética. Resultados: os trabalhadores relataram que a pandemia agravou uma histórica, crônica e precária condição de trabalho e saúde, marcada pelo aumento da sobrecarga laboral, falta de equipamentos de proteção individual e de recursos materiais para a assistência, escassez de profissionais e desvalorização da categoria, o que gerou uma percepção de desumanização no trabalho ao se sentiram como “máquinas” ou “números”. O sofrimento mental diante do risco de contaminação, da morte frequente de pacientes, colegas de trabalho e familiares, da falta de apoio da sociedade em relação às medidas protetivas e das cobranças crescentes por desempenho e produtividade geraram sintomas de ansiedade, depressão e estresse. Conclusão: a pandemia de COVID-19 intensificou a precarização do trabalho da enfermagem e o sofrimento mental dos profissionais, o que torna urgente a busca de melhorias nas condições de trabalho e de promoção da saúde, fundamentais à proteção e à dignidade dos trabalhadores.