Miracle-dz/UniFAR-FTUs huggingface dataset:
De Novo Transcriptome Assembly, Gene Annotations, and Characterization of Functional Profiling Reveal Key Genes for Lead Alleviation in the Pb Hyperaccumulator Greek Mustard (Hirschfeldia incana L.)

Lead (Pb) contamination is a widespread environmental problem due to its toxicity to living organisms. Hirschfeldia incana L. , a member of the Brassicaceae family, commonly found in the Mediterranean regions, is characterized by its ability to tolerate and accumulate Pb in soils and hydroponic cultures. This plant has been reported as an excellent model to assess the response of plants to Pb. However, the lack of genomic data for H. incana hinders research at the molecular level. In the present study, we carried out RNA deep transcriptome sequencing (RNA-seq) of H. incana under two conditions, control without Pb(NO3)2 and treatment with 100 µM of Pb(NO3)2 for 15 days. A total of 797.83 million reads were generated using Illumina sequencing technology. We assembled 77,491 transcript sequences with an average length of 959 bp and N50 of 1330 bp. Sequence similarity analyses and annotation of these transcripts were performed against the Arabidopsis thaliana nr protein database, Gene Ontology (GO), and KEGG databases. As a result, 13,046 GO terms and 138 KEGG maps were created. Under Pb stress, 577 and 270 genes were differentially expressed in roots and aboveground parts, respectively. Detailed elucidation of regulation of metal transporters, transcription factors (TFs), and plant hormone genes described the role of actors that allow the plant to fine-tune Pb stress responses. Our study revealed that several genes related to jasmonic acid biosynthesis and alpha-linoleic acid were upregulated, suggesting these components’ implication in Hirschfeldia incana L responses to Pb stress. This study provides data for further genomic analyses of the biological and molecular mechanisms leading to Pb tolerance and accumulation in Hirschfeldia incana L.
STELLAR ROTATION PERIODS OF THE KEPLER OBJECTS OF INTEREST: A DEARTH OF CLOSE-IN PLANETS AROUND FAST ROTATORS

We present a large sample of stellar rotation periods for Kepler Objects of Interest, based on three years of public Kepler data. These were measured by detecting periodic photometric modulation caused by star spots, using an algorithm based on the autocorrelation function of the light curve, developed recently by McQuillan, Aigrain & Mazeh (2013). Of the 1919 main-sequence exoplanet hosts analyzed, robust rotation periods were detected for 737. Comparing the detected stellar periods to the orbital periods of the innermost planet in each system reveals a notable lack of close-in planets around rapid rotators. It appears that only slowly spinning stars with rotation periods longer than 5–10 days host planets on orbits shorter than 2 or 3 days, although the mechanism(s) that lead(s) to this is not clear.
Monitoring the Dynamic Changes in Vegetation Cover Using Spatio-Temporal Remote Sensing Data from 1984 to 2020

Anthropogenic activities and natural climate changes are the central driving forces of global ecosystems and agriculture changes. Climate changes, such as rainfall and temperature changes, have had the greatest impact on different types of plant production around the world. In the present study, we investigated the spatiotemporal variation of major crops (cotton, rice, wheat, and sugarcane) in the District Vehari, Pakistan, from 1984 to 2020 using remote sensing (RS) technology. The crop identification was pre-processed in ArcGIS software based on Landsat images. After pre-processing, supervised classification was used, which explains the maximum likelihood classification (MLC) to identify the vegetation changes. Our results showed that in the study area cultivated areas under wheat and cotton decreased by almost 5.4% and 9.1% from 1984 to 2020, respectively. Vegetated areas have maximum values of NDVI (> 0.4), and built-up areas showed fewer NDVI values (0 to 0.2) in the District Vehari. During the Rabi season, the temperature was increased from 19.93 °C to 21.17 °C. The average temperature was calculated at 34.28 °C to 35.54 °C during the Kharif season in the District Vehari. Our results showed that temperature negatively affects sugarcane, rice, and cotton crops during the Rabi season, and precipitation positively affects sugarcane, rice, and cotton crops during the Kharif season in the study area. Accurate and timely assessment of crop estimation and relation to climate change can give very useful information for decision-makers, governments, and planners in formulating policies regarding crop management and improving agriculture yields.
Asymmetric mass models of disk galaxies - I. Messier 99

Mass models of galactic disks traditionnally rely on axisymmetric density and rotation curves, paradoxically acting as if their most remarkable asymmetric features, like e.g. lopsidedness or spiral arms, were not important. In this article, we relax the axisymmetry approximation and introduce a methodology that derives 3D gravitational potentials of disk-like objects and robustly estimates the impacts of asymmetries on circular velocities in the disk mid-plane. Mass distribution models can then be directly fitted to asymmetric line-of-sight velocity fields. Applied to the grand-design spiral M99, the new strategy shows that circular velocities are highly non-uniform, particularly in the inner disk of the galaxy, as a natural response to the perturbed gravitational potential of luminous matter. A cuspy inner density profile of dark matter is found in M99, in the usual case where luminous and dark matter share the same centre. The impact of the velocity non-uniformity is to make the inner profile less steep, though the density remains cuspy. On another hand, a model where the halo is core-dominated and shifted by 2.2-2.5 kpc from the luminous mass centre is more appropriate to account for most of the kinematical lopsidedness evidenced in the velocity field of M99. However, the gravitational potential of luminous baryons is not asymmetric enough to explain the kinematical lopsidedness of the innermost regions, irrespective of the density shape of dark matter. This discrepancy points out the necessity of an additional dynamical process in these regions, maybe a lopsided distribution of dark matter.
A Comparative Study of the Structure of the Intonation Unit in English, Japanese, and Mandarin Chinese

1 A: I could `never [^do it], CLS (fc) 2 B: [We=ll], N/A N/A 3 A: . [2 in ^Seattle. NON-CLS (other) 4 . but in ^Riverside, NON-CLS (other) 5 . when 2] you go ^outside, CLS (fc) 6 B: [2 ^anything is `possible, CLS (fc) 7 . in ^Riverside 2]. NON-CLS (other) 8 A: . and the ^air, NON-CLS (np) 9 . sort of `wraps ^around you, CLS (sc) 10 . except it's `not ^fog, CLS (fc) 11 . it's `too ^hot for that, CLS (fc)
Characteristics of Hepatitis B Virus Genotype and Sub-Genotype in Hepatocellular Cancer Patients in Vietnam

Untreated chronic hepatitis B virus (HBV) infection can lead to chronic liver disease and may progress to cirrhosis or hepatocellular carcinoma (HCC). HBV infection has been prevalent in Vietnam, but there is little information available on the genotypes, sub-genotypes, and mutations of HBV in patients with HBV-related HCC confirmed by histopathological diagnosis. We studied the molecular characteristics of HBV and its genetic variants in Vietnamese HCC patients after liver tumor resection. We conducted a descriptive cross-sectional study on 107 HBV-related HCC hospitalized patients from October 2018 to April 2019. The specimens collected included EDTA anticoagulant blood and liver tissues. Extracted HBV DNA was subjected to whole genome sequencing by the Sanger method. We discovered 62 individuals (57.9%) with genotype B and 45 patients (42.1%) with genotype C, with only sub-genotypes B4 and C1. Among the mutations, the double mutation, A1762T-G1764A, had the most significant frequency (73/107 samples; 68.2%) and was higher in genotype C than in genotype B (p < 0.001). The most common genotypes found in HCC patients in this investigation were B and C, with sub-genotypes B4 and C1 for each. The prevalence of genotype B4 was greater in HBV-infected Vietnamese HCC patients.
Model of Acute Liver Failure in an Isolated Perfused Porcine Liver—Challenges and Lessons Learned

Acute liver failure (ALF) is a rare but devastating disease associated with substantial morbidity and a mortality rate of almost 45%. Medical treatments, apart from supportive care, are limited and liver transplantation may be the only rescue option. Large animal models, which most closely represent human disease, can be logistically and technically cumbersome, expensive and pose ethical challenges. The development of isolated organ perfusion technologies, originally intended for preservation before transplantation, offers a new platform for experimental models of liver disease, such as ALF. In this study, female domestic swine underwent hepatectomy, followed by perfusion of the isolated liver on a normothermic machine perfusion device. Five control livers were perfused for 24 h at 37 °C, while receiving supplemental oxygen and nutrition. Six livers received toxic doses of acetaminophen given over 12 h, titrated to methemoglobin levels. Perfusate was sampled every 4 h for measurement of biochemical markers of injury (e.g., aspartate aminotransferase [AST], alanine aminotransferase [ALT]). Liver biopsies were taken at the beginning, middle, and end of perfusion for histological assessment. Acetaminophen-treated livers received a median dose of 8.93 g (8.21–9.75 g) of acetaminophen, achieving a peak acetaminophen level of 3780 µmol/L (3189–3913 µmol/L). Peak values of ALT (76 vs. 105 U/L; p = 0.429) and AST (3576 vs. 4712 U/L; p = 0.429) were not significantly different between groups. However, by the end of perfusion, histology scores were significantly worse in the acetaminophen treated group (p = 0.016). All acetaminophen treated livers developed significant methemoglobinemia, with a peak methemoglobin level of 19.3%, compared to 2.0% for control livers (p = 0.004). The development of a model of ALF in the ex vivo setting was confounded by the development of toxic methemoglobinemia. Further attempts using alternative agents or dosing strategies may be warranted to explore this setting as a model of liver disease.
Systematic Review and Meta-Analysis on the Sensitivity and Specificity of 13C/14C-Urea Breath Tests in the Diagnosis of Helicobacter pylori Infection

Helicobacter pylori (H. pylori) continues to be a major health problem worldwide, causing considerable morbidity and mortality due to peptic ulcer disease and gastric cancer. The aim of the present systematic review and meta-analysis was to determine the sensitivity and specificity of 13C/14C-urea breath tests in the diagnosis of H. pylori infection. A PRISMA systematic search appraisal and meta-analysis were conducted. A systematic literature search of PubMed, Web of Science, EMBASE, Scopus, and Google Scholar was conducted up to August 2022. Generic, methodological and statistical data were extracted from the eligible studies, which reported the sensitivity and specificity of 13C/14C-urea breath tests in the diagnosis of H. pylori infection. A random effect meta-analysis was conducted on crude sensitivity and specificity of 13C/14C-urea breath test rates. Heterogeneity was assessed by Cochran’s Q and I2 tests. The literature search yielded a total of 5267 studies. Of them, 41 articles were included in the final analysis, with a sample size ranging from 50 to 21857. The sensitivity and specificity of 13C/14C-urea breath tests in the diagnosis of H. pylori infection ranged between 64–100% and 60.5–100%, respectively. The current meta-analysis showed that the sensitivity points of estimate were 92.5% and 87.6%, according to the fixed and random models, respectively. In addition, the specificity points of estimate were 89.9% and 84.8%, according to the fixed and random models, respectively. There was high heterogeneity among the studies (I2 = 98.128 and 98.516 for the sensitivity and specificity, respectively, p-value < 0.001). The 13C/14C-urea breath tests are highly sensitive and specific for the diagnosis of H. pylori infection.
INVESTIGATIONS OF PROTOSTELLAR OUTFLOW LAUNCHING AND GAS ENTRAINMENT: HYDRODYNAMIC SIMULATIONS AND MOLECULAR EMISSION

We investigate protostellar outflow evolution, gas entrainment, and star formation efficiency using radiation-hydrodynamic simulations of isolated, turbulent low-mass cores. We adopt an X-wind launching model, in which the outflow rate is coupled to the instantaneous protostellar accretion rate and evolution. We vary the outflow collimation angle from θ = 0.01–0.1 and find that even well-collimated outflows effectively sweep up and entrain significant core mass. The Stage 0 lifetime ranges from 0.14–0.19 Myr, which is similar to the observed Class 0 lifetime. The star formation efficiency of the cores spans 0.41–0.51. In all cases, the outflows drive strong turbulence in the surrounding material. Although the initial core turbulence is purely solenoidal by construction, the simulations converge to approximate equipartition between solenoidal and compressive motions due to a combination of outflow driving and collapse. When compared to simulation of a cluster of protostars, which is not gravitationally centrally condensed, we find that the outflows drive motions that are mainly solenoidal. The final turbulent velocity dispersion is about twice the initial value of the cores, indicating that an individual outflow is easily able to replenish turbulent motions on sub-parsec scales. We post-process the simulations to produce synthetic molecular line emission maps of 12CO, 13CO, and C18O and evaluate how well these tracers reproduce the underlying mass and velocity structure.
A STELLAR WIND ORIGIN FOR THE G2 CLOUD: THREE-DIMENSIONAL NUMERICAL SIMULATIONS

We present three-dimensional, adaptive mesh refinement simulations of G2, a cloud of gas moving in a highly eccentric orbit toward the galactic center. We assume that G2 originates from a stellar wind interacting with the environment of the Sgr A* black hole. The stellar wind forms a cometary bubble which becomes increasingly elongated as the star approaches periastron. A few months after periastron passage, streams of material begin to accrete on the central black hole with accretion rates M☉ yr−1. Predicted Brγ emission maps and position–velocity diagrams show an elongated emission resembling recent observations of G2. A large increase in luminosity is predicted by the emission coming from the shocked wind region during periastron passage. The observations, showing a constant Brγ luminosity, remain puzzling, and are explained here assuming that the emission is dominated by the free-wind region. The observed Brγ luminosity (∼8 × 1030 erg s−1) is reproduced by a model with a vw = 50 km s−1 wind velocity and a 10−7 M☉ yr−1 mass-loss rate if the emission comes from the shocked wind. A faster and less dense wind reproduces the Brγ luminosity if the emission comes from the inner, free-wind region. The extended cometary wind bubble, largely destroyed by the tidal interaction with the black hole, reforms a few years after periastron passage. As a result, the Brγ emission is more compact after periastron passage.
Cosmological dynamics and dark energy from nonlocal infrared modifications of gravity

We study the cosmological dynamics of a recently proposed infrared modification of the Einstein equations, based on the introduction of a nonlocal term constructed with m2gμν□-1R, where m is a mass parameter. The theory generates automatically a dynamical dark energy component, that can reproduce the observed value of the dark energy density without introducing a cosmological constant. Fixing m so to reproduce the observed value ΩDE≃0.68, and writing wDE(a) = w0+(1-a)wa, the model provides a neat prediction for the equation of state parameters of dark energy, w0≃-1.042 and wa≃-0.020, and more generally provides a pure prediction for wDE as a function of redshift. We show that, because of some freedom in the definition of □-1, one can extend the construction so to define a more general family of nonlocal models. However, in a first approximation this turns out to be equivalent to adding an explicit cosmological constant term on top of the dynamical dark energy component. This leads to an extended model with two parameters, ΩΛ and m. Even in this extension the EOS parameter w0 is always on the phantom side, in the range -1.33 ≲w0≤-1, and there is a prediction for the relation between w0 and wa.
The Analysis of Barriers to the Use of Physics Practicum Applications

This research aims to analyze the conflicts or obstacles faced by students and physics teachers in carrying out physics practicum activities at SMP Negeri 5 Jambi City. This Research Means Narrative Research Using Mix Method. The sample in this study was 35 students and two subject physics teachers. Data Collection Using Angel Teachers and Students About Physics Practicum Applications And Interview Guide Sheets To Teachers. This study uses narrative statistical analysis techniques for quantitative data and uses the example of Miles and Huberman for qualitative data. The research results based on the questionnaire of students and teachers showed that the implementation and assessment of the physics practicum and student motivation were in a suitable category, while the teacher's motivation was in a bad category. Then from what will happen in the interview, problems are obtained in the implementation of the physics practicum, namely the laboratory room, which is still used as a shared room, not enough time to carry out the practicum, the lack of teacher motivation, and the number of practicum senses that are less evenly distributed.
THE MASS OF THE BLACK HOLE IN THE X-RAY BINARY NOVA MUSCAE 1991

The optical counterpart of the black hole soft X-ray transient Nova Muscae 1991 has brightened by ΔV ≈ 0.8 mag since its return to quiescence 23 yr ago. We present the first clear evidence that the brightening of soft X-ray transients in quiescence occurs at a nearly linear rate. This discovery, and our precise determination of the disk component of emission obtained using our simultaneous photometric and spectroscopic data, has allowed us to identify and accurately model archival ellipsoidal light curves of the highest quality. The simultaneity, and the strong constraint it provides on the component of disk emission, is a key element of our work. Based on our analysis of the light curves, and our earlier measurements of the mass function and mass ratio, we have obtained for Nova Muscae 1991 the first accurate estimates of its systemic inclination deg, and black hole mass . Based on our determination of the radius of the secondary, we estimate the distance to be kpc. We discuss the implications of our work for future dynamical studies of black hole soft X-ray transients.
FACTORS AFFECTING ACCEPTANCE OF AUDITOR'S DYSFUNCTIONAL BEHAVIOR AND CONSEQUENCES ON THE QUALITY OF AUDIT RESULTS AT PUBLIC ACCOUNTING OFFICES IN BALI

This study aims to analyze work stress, time budget pressure, locus of control and auditor personality type on auditor dysfunctional behavior and its consequences on the quality of audit results. The theories used in this research are Theory of Attitudinal Change. The independent variables of this research are work stress, time budget pressure, locus of control and personality type. The dependent variable is the quality of the audit results. Also, the mediating variable is auditor dysfunctional behavior. This research was conducted at a registered public accounting firm in Bali using a questionnaire distributed to auditors who at least become team leaders in audit assignments. The data analysis technique used is the Partial Least Square (PLS) approach. The results showed that audit time budget pressure and locus of control had a positive effect on work stress. Furthermore, work stress, audit time budget pressure and locus of control have a positive effect on audit quality reduction behavior. The results also show that work stress, time budget pressure, external locus of control and personality type have a positive effect on auditor dysfunctional behavior. Furthermore, the dysfunctional behavior of auditors has a negative effect on the quality of audit results.
EVALUATION OF MELATONIN AND HYALURONIC ACID IN MAXILLARY SINUS AUGMENTATION (A RANDOMIZED CONTROLLED CLINICAL TRIAL)

INTRODUCTION: Rehabilitation of maxillary posterior region with endosseous implants is complicated in the presence of insufficient residual alveolar ridge and maxillary sinus pneumatization. New bone formation without any bone grafting materials has achieved promising results in sinus augmentation. Melatonin and hyaluronic acid suggest satisfying results in enhancing bone defect repair, osseointegration of dental implants. OBJECTIVES: To evaluate the effectiveness of melatonin and hyaluronic acid combination on bone regeneration in maxillary sinus augmentation. MATERIALS AND METHODS: 16 patients with missed maxillary posterior teeth indicated for sinus augmentation were divided equally into; group I, received melatonin and hyaluronic acid after sinus membrane elevation with simultaneous implant placement, and group II, did not receive any materials after the procedure. Clinical evaluation were done for pain, swelling, complications, and implant stability and radiographically for vertical bone height gain, bone density, and marginal bone level. RESULTS: There was a significant increase in bone density on the 1st and 6th months postoperatively in each group and between both groups ( p ≤ 0.05 ). Regarding vertical bone height gain and implant stability, there was significant increase in each group, whereas the increase was insignificant between both groups. Marginal bone level difference between both groups was insignificant. Sinus membrane perforation occurred in one case only which did not affect the procedure completion; and there was no failure or complications along the follow up periods. CONCLUSION: Melatonin and hyaluronic acid could have a considerable properties regarding bone density in sinus augmentation without complications. ___________________________________________________________________________
An Investigation of the Relationship between First Year Secondary Stage Students’ Oral Performance and their Productive Classroom Behavior

This study investigates the relationship between first year secondary stage students’ oral performance and their productive classroom behavior. The study sample were 'sixty' first year secondary stage students at Governorate, Egypt . The study sample’s oral performance was measured by an oral performance test; their productive classroom behavior was measured by using a productive classroom behavior scale. Both instruments were prepared by the researcher. Findings showed a statistically significant correlation between the scores obtained from the oral performance of all participants and the productive classroom behavior scale as a whole and with regard to its four dimensions. There was a direct correlation between the study sample's oral-performance (who obtained the highest scores) and the productive classroom behavior regarding the attention , discipline , and participation dimensions except for the turn-management dimension. Also, there was no significant correlation between the scores obtained from the administration of the study sample's oral performance (who obtained the lowest scores) and the productive classroom behavior scale regarding its four dimensions.