Miracle-dz/UniFAR-FTUs huggingface dataset:
Allosteric stabilization of calcium and phosphoinositide dual binding engages three synaptotagmins in fast exocytosis

Synaptic communication relies on the fusion of synaptic vesicles with the plasma membrane, which leads to neurotransmitter release. This exocytosis is triggered by brief and local elevations of intracellular Ca2+ with remarkably high sensitivity. How this is molecularly achieved is unknown. While synaptotagmins confer the Ca2+ sensitivity of neurotransmitter exocytosis, biochemical measurements reported Ca2+ affinities too low to account for synaptic function. However, synaptotagmin’s Ca2+ affinity increases upon binding the plasma membrane phospholipid PI(4,5)P2 and, vice versa, Ca2+-binding increases synaptotagmin’s PI(4,5)P2 affinity, indicating a stabilization of the Ca2+/PI(4,5)P2 dual-bound syt. We here devise a molecular exocytosis model based on this positive allosteric stabilization and the assumptions that (1.) synaptotagmin Ca2+/PI(4,5)P2 dual binding lowers the energy barrier for vesicle fusion and that (2.) the effect of multiple synaptotagmins on the energy barrier is additive. The model, which relies on biochemically measured Ca2+/PI(4,5)P2 affinities and protein copy numbers, reproduced the steep Ca2+ dependency of neurotransmitter release. Our results indicate that each synaptotagmin dual binding Ca2+/PI(4,5)P2 lowers the energy barrier for vesicle fusion by 4.85 kBT and that allosteric stabilization of this state enables the synchronized engagement of three synaptotagmins for fast exocytosis. Furthermore, we show that mutations altering synaptotagmin’s allosteric properties may show dominant-negative effects, even though synaptotagmins act independently on the energy barrier, and that dynamic changes of local PI(4,5)P2 (e.g. upon vesicle movement) dramatically impact synaptic responses. We conclude that allosterically stabilized Ca2+/PI(4,5)P2 dual binding enables synaptotagmins to exert their coordinated function in neurotransmission.
Development of a mobile application for identification of grapevine 
(Vitis vinifera L.) cultivars via deep learning

Traditional vine variety identification methods usually rely on the sampling of vine leaves followed by physical, physiological, biochemical and molecular measurement, which are destructive, time-consuming, labor-intensive and require experienced grape phenotype analysts. To mitigate these problems, this study aimed to develop an application (App) running on Android client to identify the wine grape automatically and in real-time, which can help the growers to quickly obtain the variety information. Experimental results showed that all Convolutional Neural Network (CNN) classification algorithms could achieve an accuracy of over 94% for twenty-one categories on validation data, which proves the feasibility of using transfer deep learning to identify grape species in field environments. In particular, the classification model with the highest average accuracy was GoogLeNet (99.91%) with a learning rate of 0.001, mini-batch size of 32 and maximum number of epochs in 80. Testing results of the App on Android devices also confirmed these results. Keywords: deep learning, mobile phone, grapevine cultivar, vine leaf image, identification, Vitis vinifera L. DOI: 10.25165/j.ijabe. 20211405.6593 Citation: Liu Y X, Shen L, Su J Y, Lu N, Fang Y L, Liu F, et al. Development of a mobile application for identification of grapevine (Vitis vinifera L.) cultivars via deep learning. Int J Agric & Biol Eng, 2021; 14(5): 172–179.
Outbreak of Multidrug-Resistant OXA-232-Producing ST15 Klebsiella pneumoniae in a Teaching Hospital in Wenzhou, China

Background OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) has the potential to become the “third epidemic” of carbapenem-resistant Klebsiella strain after KPC-2 and NDM in China. We investigated the first outbreak of CRKP in the First Affiliated Hospital of Wenzhou Medical University. Methods We collected 610 clinical isolates of CRKP from the First Affiliated Hospital of Wenzhou Medical University between January 2019 and September 2020 and screened them by Polymerase Chain Reaction (PCR). The multilocus sequence typing and pulsed-field gel electrophoresis were used to determine the genetic relatedness of the strains. The antimicrobial susceptibility test was performed to determine the drug resistance of the clinical isolates. The molecular mechanism underlying carbapenem resistance was elucidated by performing PCR and conjugation experiments. The virulence potential of the strains was determined by the string test, detection of virulence-associated genes and capsular serotypes, and Galleria mellonella larval infection model. Results Between September 2019 and May 2020, 26 OXA-232-producing CRKP were obtained from 12 patients in our hospital. Ten patients were hospitalized in the intensive care units (ICU) and the overall mortality of the inpatients involved in the outbreak was 50% (6/12). Epidemiological investigations reported that all the OXA-232-producing CRKP strains belonged to the sequence type ST15 and can be clonally transmitted among the inpatients in the ICU. All the strains had low virulence and were resistant to commonly used clinical antibiotics except for ceftazidime/avibactam, colistin, and tigecycline. The OXA-232-producing CRKP was sensitive to triclosan and chlorhexidine, and its eradication from our hospital can be achieved by the use of disinfectants in the ICU. Conclusion In our study, OXA-232-producing CRKP isolates appeared to be clonally transmitted and the sequence type ST15 was responsible for the outbreak. Therefore, effective measurements for the infection control of CRKP are urgently needed to prevent its epidemic in the nearby region in the future.
Effect of Communication Skills Training Program for Head Nurses on Interpersonal Relations and Nurses' Organizational Commitment

Background : Nurses are the most precious assets of any health-care institution, thus it's critical to understand their behavior. In this regard, the present study was carried out in order to examine the effect of communication skills training program for head nurses on interpersonal relations and nurses' organizational commitment. This study was carried out at Zagazig University Hospitals, using quasi experimental design. A convenience sample of 50 head nurses and simple random sample of 221 staff nurse from the previously mentioned setting. Tools : A questionnaire sheet was used to collect data for this study that was composed of three parts; Organization Communication Questionnaire, Fundamental interpersonal relation orientation behavior questionnaires and organizational commitment scale. Results revealed that there was significant improvement in head nurses' communication skills mean score after the program implementation (P < 0.01). As well, there was statistically significant enhancement in their interpersonal relations with nurses mean score (P < 0.01). Additionally, there was a statistically significant improvement in nurses' organizational commitment after the program implementation (P < 0.01). Conclusion : communication skills training program for head nurses is effective in improving interpersonal relations and commitment of their nurses. Recommendation : hospital administration should establish training activities to offer nurses with key skills such as active listening, accurate feedback, and problem-solving techniques.
Natural compounds obtained from plants as alternatives for meat and meat products preservation

The purpose of this review is to demonstrate the findings on the use of natural compounds as preservatives in meat and meat products as a healthier and safer alternative compared to the use of synthetic preservatives. Due to its biological composition, meat and its derivatives are susceptible to spoilage, a process that can be delayed with the use of synthetic food preservatives. However, it is known that some products can trigger undesirable effects on consumer health. In recent years, there has been a greater demand for products obtained naturally or directly from a biological organism without laboratory interference in their composition, in order to reduce risks to the consumer. The most promising compounds belong to the groups of phenolic and flavonoid compounds, which have a proven, positive effect on oxidation and microbial development. Findings on the use of natural compounds as preservatives in meat and meat products demonstrate an alternative to increased shelf life without risking consumer health.
Investigating Emergency Nurses’ Awareness on the Differentiation Between Acute Methanol and Ethanol Intoxication

Background: Alcohol intoxication is among the leading and preventable causes of death, disability, and injury in numerous societies. Ethanol and methanol are the most commonly used types of alcohol. Increasing nurses’ awareness about the difference between intoxication with these two alcohol types will prevent the occurrence of dangerous and deadly complications of intoxication. We investigated the awareness of the nurses respecting the difference between acute methanol and ethanol intoxication in clinical manifestations, diagnosis, and treatment. Methods: A cross-sectional study was performed in 2020 on the emergency nurses from two educational hospitals. A total of 100 nurses participated in this study. The data collection tool was a researcher-made questionnaire, including demographic characteristics and questions concerning the identification and differentiation of acute methanol from ethanol intoxication. Moreover, the obtained data were analyzed using SPSS v. 20 at the significance level of P<0.05. Results: The present research results suggested that 21% of the examined nurses had a low level of awareness (<7) and 79% had a moderate level of awareness (8-13). The mean score of awareness was measured as 8.71 (range: 2-12). Nurses’ awareness was not related to age, gender, the duration of working experience, and educational level (P>0.05). Conclusion: The awareness of nurses regarding alcohol poisoning is moderate. Due to the importance of differentiating ethanol from methanol toxicity, considering the outcomes of the patients, nurses’ awareness should be increased.
Plum Drying Optimization: An Assessment Through Osmo-Dehydration

The aim of the study was to formulate the stable combination of osmotic agent for dehydration/drying of plum fruit based on physiochemical parameters, moisture, water activity and microbial load. The treatments were made DP0 (control), DP1 (40% glucose), DP2 (40% sucrose), DP3 (40% honey), DP4 (20% glucose + 20% sucrose), DP5 (20% glucose + 20% honey), DP6 (20% sucrose + 20% honey), DP7 (13.3% glucose + 13.3% sucrose +13.3% honey). The plum slices were immersed in these treatments for 24 hrs and were dried in cabinet drier at 600C. Results indicated that a significant increase (P≤0.01) was observed in TSS (from 54.38-65.68), acidity (from 1.12-1.58%) and reducing sugar (from 51.4761.49%). A significant decrease (P≤0.01) was examined in pH (from 3.70-2.94), ascorbic acid (from 11.62-8.34mg/100g), moisture (from 13.17 – 10.12 %), and non-reducing sugar (from 28.73-24.18%). The mean score of 10 judges showed a significant decrease (P≤0.01) in color (from 7.28-6.06), flavor (from 7.29-5.26), texture (from 7.76-7.24) and overall acceptability (from 6.98-5.92). the microbial load and water activity significant decreased from 2.191. 79 log cfu/g and 0.560. 50 respectively. Statistical analysis shows that storage interval and treatment had a significant (P≤0.01) effect on physicochemical and sensory properties of dried plum slices. The results indicated that simple drying process is not adequate and that the addition of osmotic agent and preservative would be an additional method to ensure safety, stability and quality of force dried fruit especially plum. The current study found DP1 followed by DP4 were of best quality and stable according to microbial load among treatments
The Effectiveness of a Computerized Simulation-Based Educational Program in Developing Reading and speaking skills for the 3rd Grade

This current study aims to investigate the effectiveness of a Computerized Simulation-Based Educational Program In Developing Reading and Speaking skills for the 3rd Grade Students in The English Language. This study attempted to answer the following questions: What is the effectiveness of a Computerized Simulation-Based Educational program on developing student's reading and speaking skills between the experimental group (which was studied using the computerized simulation-based educational program) and the control group (which was studied in the usual way)? Are there any statistical significant differences at (a<0.05) in the achievement between the students' performance in the two skills due to the Computerized Simulation-Based Educational program? The population of the study consisted of 365 students who are studying at the private schools in Al-Karak directorate and sample of the study consisted of (30) male and female of the third grade students and they were selected by the Intentional method. And it was divided into two groups; control group (15 students ) were taught in the usual way and experimental group (15 students) were taught with a Computerized Simulation-Based Educational program . The observation cards were constructed with the sub-skills of the three skills, pre/post tests were constructed and applied and a Computerized Simulation-Based Educational program where validity and reliability were ensured for the previous tools. The results of the study indicated that there were significant statistical differences at (α≤0.05) between the control and the experimental group in developing student's Reading, and Speaking skills in favor of the experimental group. In the light of the findings of this study, the researcher recommends the following : The Ministry of Education in Jordan should increase attention and focus on employing technology and computerized educational programs based on simulations in teaching different skills And the use of this technology in the classroom of schools and conducting more studies on other samples and other subjects.
EFFECT OF SOME MACRO AND MICRONUTRIENTS ON GROWTH, YIELD AND CHEMICAL CONSTITUENTS OF CHAMOMILE PLANTS (Matricaria chamomilla L.) GROWN UNDER NEWLY RECLAIMED SOIL CONDITIONS.

The present study was carried out in field during the growing seasons 2004/2005 and 2005/2006 to investigate the effect of macronutrients at the rates of N (75 and 100 kg /fed). P2O5 (32 and 50 kg./fed.) and K2O (24 and 36 kg./fed.) and the mixture of micronutrients (Fe, Mn and Zn at the rate of 2%) on growth, yield and chemical constituents of chamomile plants. The data obtained from the study show that the treated plants with the high rates of macronutrients (NPK) either alone or in combination with each others increased all the studied characters such as growth parameters (plant height, number of branches, fresh and dry weights of plant), yield (number of inflorescences/plant, fresh and dry weights of inflorescences/plant and dry yield /fed.) and some chemical constituents such as (N, P, K, Fe, Mn, Zn, pigments and total carbohydrates). Also yield of oil (oil percentage, oil yield/ plant and fedann) Generally, the maximum increase of the previous characters was obtained by the treatment which contain the high rate of NPK fertilizers (100, 50 and 36 kg/fed., respectively) either alone or in combination with spraying chamomile plants with micronutrients (Fe, Zn and Mn) at the concentration of 2%. Therefore, under newly reclaimed soil conditions prefer fertilization chamomile plants with (100, 50 and 36 kg/fed. from NPK fertilizers, respectively in combination with micronutrients (Fe, Zn and Mn) at the concentration 2% to obtain the good yield from the plants.
The Eruption Patterns of the Teeth of Nigerian Local Pigs (Sus scrofa): Profile of Deciduous Teeth

The pigs, domesticated about 6000 years ago, are highly prolific omnivores belonging to the Family Suidae and Order artiodactyla along with their other hooved counterparts. They qualify as models for various translational research studies (including dental studies) because of their anatomical and physiological similarities to humans. There is however a dearth of information as regards the deciduous dental eruption profile (sequence and timing of eruption) of the Nigerian local pigs (NLP). This study was designed to determine the deciduous eruption profile in the NLP to assist in the management of the breed and in a bid to making the NLP more obvious as suitable animal models in dental research. A total of 51 healthy piglets (from postnatal day 1) were used for this. Their oral cavities were examined every other day for 29 weeks for signs of dental eruption. Looking at Mandibular (Md) and Maxillary (Mx) eruptions for Incisors (I), Canines (C), Premolars (P) and Molars (M). The modular sequence in the NIP was Md/ Mx I3 (at birth), C (at birth) →Md I1→Md P3→MxP3 →MxI1 → Md I2 →MdP4 →MxP4 →MxP2→ Md I2 →MxI2. It was also observed that variations in eruption timing exist amongst individuals and sexes while variation in eruption sequence exists between NLP and various breeds of pigs based on the literature. The results of this study will be particularly important to farmers, and researchers into NLP especially those using pigs in Nigeria as a model for translation research.
An Examination of the Relationship Between Agricultural Incentives and Agricultural Added Value: Panel Simulated Equations System Analysis for New Industrialized Countries

Today, same as before, agriculture sector benefits from the incentives provided by the governments of underdeveloped and developing countries as well as the governments of developed countries in various ways. The protection and development of the sector is one of the most important factors in the background of the mentioned incentives. On the other hand, the fact that the agriculture sector has a strategic prescription for the continuation of the existence of the nations, increases the necessity of incentives offered to the sector one more time. The study analyzed the relationship between agricultural incentives and agricultural value added among 5 countries (Turkey, South Africa, Mexico, China and Brazil), which are the members of the group of newly industrialized countries for the years 2000 and 2016. In the analysis, the panel data simultaneous equations systems is used. The mutual relationship between agricultural incentives and agricultural value added was established with the help of the simultaneous equations method. According to the results of the analysis, the increase in the agricultural incentives has a positive effect on the agricultural value added. Giriş Tarım, insanlık tarihinin ilkel iktisadi aktivitelerinden en karmaşık iktisadi aktivitelerin gerçekleştirildiği günümüze kadar önemini korumuştur. Modern dünyanın ekonomik faaliyetlerinin henüz sektörel olarak ayrıştırılmadığı dönemlerde, tarımsal faaliyetler ekonomik yapının ve ticaretin ana unsurunu oluşturmuştur. Tarım sektörünün ekonomik aktivitelerin ana unsurunu oluşturması, her hangi bir ülkede tarım alanında uygulanan politikaların aynı zamanda o ülkenin ekonomi politikalarının da en önemli belirleyicisi olduğunu gözler önüne sermiştir (Sumner, Alston&Glauber,2010:403). Günümüze birçok ülke, bir yandan tarım sektörünün sahip olduğu stratejik önemden dolayı diğer yandan da ülke içi ekonomik dinamikler nedeniyle tarım sektörüne özendirme ve geliştirme amaçlı çeşitli teşvikler uygulamaktadır. Çeşitli amaçlar doğrultusunda tarım sektörüne uygulanan teşvikler ihracat teşvikleri, yurtiçi tarımsal ürün Mehmet Akyol Dr. Öğr. Üyesi, Gümüşihane Üniversitesi,İİBF, İktisat Bölümü, mehmet_akyol81@hotmail.com Article Type / Makale Türü Research Article / Araştırma Makalesi Anahtar Kelimeler Tarımsal teşvik, Tarımsal katma değer, Panel eşanlı denklemler
New Older Users’ Attitudes Toward Social Networking Sites and Loneliness: The Case of the Oldest-Old Residents in a Small Italian City

Older adults make little use of social networking sites (SNS). SNS has become essential for maintaining social contacts and countering loneliness in the current era marked by the Covid-19 pandemic. This study explores the attitudes of the oldest-old on SNS after attending a training course on SNS use. The study’s goals are to investigate their personal experiences, choices of use and to survey their views on the usefulness of SNS and its effects on mitigating loneliness for older people. The interviews were conducted in the context of the “Ageing in a Networked Society—Social Experiment Study. ” The participants, who were randomly selected for the course on SNS use, agreed to be interviewed during the post-intervention evaluation (N = 39). Results show SNS are mainly and productively used with relatives and friends. A positive view is reported for the potential impact of using SNS to counter loneliness, but mainly for socially isolated older individuals, while only a few find online contact futile. Intergenerational communication and a perspective of SNS as a leisure activity were identified as motivational factors for SNS use. Rare use or non-use are mainly related to privacy and security issues and technical difficulties. This is also the reason underlying the majority’s preference for WhatsApp over Facebook. These findings confirm the need for widespread SNS-focused online communication training interventions for seniors. On the speculative level, these results complement the existing literature by delving deeper into the perceptions of new older SNS users, a poorly studied segment of the population.
Maternal and neonatal serum lipid levels in preeclamptic females and its correlation with neonatal respiratory distress syndrome

Background: There have been biological exaggerations of lipid production among women with preeclampsia, but the function of serum lipids in the development of preeclampsia is unclear. In women with preeclampsis, high density lipoprotein (HDL) and LDL have been found to be lower in preeclampsia when plasma concentrations of triglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) have been increased. The current research aimed at evaluating maternal and newborn serum lipid levels and their association to neonatal respiratory distress syndrome in preeclamptic females. Methods: This is a case-control research that included two identical groups of preterm infants between 28-34 weeks, with preeclampsia as pre-existing status and normotensive moms. Results: In newborn moms with pre-term labour, RD was shown to be substantially greater than women with pre-eclampsia (P<0.05). We discovered that newborns with RD of mothers with pre-eclampsia and mother with child lipid profiles had higher maternal levels of total cholesterol, triglycerides and LDL, but lower levels of HDL, although this variation remained insignificant. The cord level of LDL and triglycerides in the RDS group was nevertheless lower. Conclusion: Maternal lipid levels are an indicator for RDS prediction. In pregnancies complicated by preeclampsia, higher maternal serum lipids have been found. In children and their mothers, RDS is accompanied with lipid changes.
Characterization of the complete chloroplast genome of Astragalus galactites (Fabaceae)

Abstract Astragalus galactites is a medicinal plant. The total plastome length of A. galactites is 126,117 bp. It contains a large single-copy region of 69,805 bp, two inverted repeat regions of 20,638 bp, and a small single-copy region of 15,036 bp. The cp genome contains 110 complete genes, including 75 protein-coding genes (75 PCGs), 4 ribosomal RNA genes (4 rRNAs), and 30 tRNA genes (30 tRNAs). The overall GC content of cp DNA is 33.9%, the corresponding values of the LSC, SSC, and IR regions are 33.0%, 30.4%, and 43.3% respectively. The phylogenetic tree shows that A. galactites has the closest relationship with A. laxmannii.