Miracle-dz/UniFAR-FTUs huggingface dataset:
Olig2-Induced Neural Stem Cell Differentiation Involves Downregulation of Wnt Signaling and Induction of Dickkopf-1 Expression

Understanding stem cell-differentiation at the molecular level is important for clinical applications of stem cells and for finding new therapeutic approaches in the context of cancer stem cells. To investigate genome-wide changes involved in differentiation, we have used immortalized neural stem cell (NSC) line (HB1.F3) and Olig2-induced NSC differentiation model (F3.Olig2). Using microarray analysis, we revealed that Olig2-induced NSC differentiation involves downregulation of Wnt pathway, which was further confirmed by TOPflash/FOPflash reporter assay, RT-PCR analysis, immunoblots, and immunocytochemistry. Furthermore, we found that Olig2-induced differentiation induces the expression of Dickkopf-1(Dkk1), a potent antagonist of Wnt signaling. Dkk1 treatment blocked Wnt signaling in HB1.F3 in a dosage-dependent manner, and induced differentiation into astrocytes, oligodendrocytes, and neurons. Our results support cancer stem cell hypothesis which implies that signaling pathway for self-renewal and proliferation of stem cells is maintained till the late stage of differentiation. In our proposed model, Dkk1 may play an important role in downregulating self-renewal and proliferation pathway of stem cells at the late stage of differentiation, and its failure may lead to carcinogenesis.
Applying new scheduling theory to static priority pre-emptive scheduling

The paper presents exact schedulability analyses for real-time systems scheduled at runtime with a static priority pre-emptive dispatcher. The tasks to be scheduled are allowed to experience internal blocking (from other tasks with which they share resources) and (with certain restrictions) to release jitter, such as waiting for a message to arrive. The analysis presented is more general than that previously published and subsumes, for example, techniques based on the Rate Monotonic approach. In addition to presenting the relevant theory, an existing avionics case study is described and analysed. The predictions that follow from this analysis are seen to be in close agreement with the behaviour exhibited during simulation studies.
Signals in Macaque Striate Cortical Neurons that Support the Perception of Glass Patterns

Glass patterns are texture stimuli made by pairing randomly placed dots with partners at specific offsets. The strong percept of global form that arises from the sparse local orientation cues has made these patterns the subject of psychophysical investigations, yet neuronal responses to Glass patterns have not been studied. We measured the responses of neurons in macaque striate cortex (V1) to dynamic, translational Glass patterns as a function of dot separation and dot-pair orientation. Responses were selective, but were on average more than an order of magnitude weaker than responses to sinusoidal gratings. Response and selectivity were greatest when the dot-pair orientation matched that of the preferred grating and when dot separation was between one-quarter and one-half of the spatial period of the optimal grating; changing the dot-pair separation or inverting the contrast of one of the dots radically changed the orientation selectivity. We computed the expected responses for a receptive field model to translational Glass patterns and found that the complexity of our V1 tuning curves could be understood in terms of the responses of linear filters to pairs of dots. This modeling connects our understanding of V1 receptive fields as rectified, quasi-linear filters with results from psychophysical studies of Glass patterns. Our results provide a basis for studying how subsequent visual areas integrate weak, local signals into global form percepts.
Overexpression of the Embryonic-Lethal Abnormal Vision-like Protein HuR in Ovarian Carcinoma Is a Prognostic Factor and Is Associated with Increased Cyclooxygenase 2 Expression

The human embryonic-lethal abnormal vision-like protein HuR is involved in the regulation of mRNA turnover and serves as a shuttling protein between the nucleus and the cytoplasm that stabilizes mRNAs containing adenine- and uridine-rich elements in their 3′ untranslated region. We have shown recently that expression of cyclooxygenase (COX)-2 is related to poor prognosis in ovarian carcinoma. Other studies have shown that the COX-2 mRNA contains an adenine- and uridine-rich element and is stabilized by HuR. In this study, we investigated the expression and cellular distribution of HuR in 83 primary ovarian carcinomas, 16 borderline tumors of the ovary, 3 normal ovaries, and 9 ovarian carcinoma cell lines. Expression of HuR was detected in all cell lines on the mRNA and protein level and showed a predominantly nuclear staining in OVCAR-3 cells by confocal microscopy. In an immunohistochemical evaluation of human ovarian carcinomas, HuR showed a nuclear expression in 81% of tumors. In addition, a cytoplasmic expression of HuR was observed in a subgroup of 45% of ovarian carcinomas. Nuclear as well as cytoplasmic expression of HuR was significantly increased in ovarian carcinomas compared with borderline tumors or normal ovaries. In univariate analysis, a significant association between cytoplasmic HuR expression and increased COX-2 expression (P = 0.025) as well as between histological grade (P = 0.008) and mitotic activity (P = 0.002) was observed, although nuclear expression of HuR was not correlated with COX-2 expression or other clinicopathological parameters. In Kaplan-Meier survival analysis, increased cytoplasmic expression of HuR was a significant prognostic indicator for progression-free survival (P = 0.03) as well as overall survival (P = 0.007). In multivariate analysis using the Cox regression model, cytoplasmic expression of HuR was an independent prognostic parameter for reduced overall survival with a relative risk of 2.62 (95% confidence interval, 1.32–5.19). Our results suggest that there is a dysregulation of cellular distribution of the mRNA stability factor HuR in a subset of invasive ovarian carcinomas. This dysregulation appears to result in an increased expression of COX-2, an increased proliferative rate, and may lead to a reduced survival time. Additional studies are required to analyze the downstream effects of increased cytoplasmic expression of HuR. In addition, it would be interesting to investigate the prognostic role of increased cytoplasmic expression of HuR in prospective studies.
Darwin phones: the evolution of sensing and inference on mobile phones

We present Darwin, an enabling technology for mobile phone sensing that combines collaborative sensing and classification techniques to reason about human behavior and context on mobile phones. Darwin advances mobile phone sensing through the deployment of efficient but sophisticated machine learning techniques specifically designed to run directly on sensor-enabled mobile phones (i.e., smartphones). Darwin tackles three key sensing and inference challenges that are barriers to mass-scale adoption of mobile phone sensing applications: (i) the human-burden of training classifiers, (ii) the ability to perform reliably in different environments (e.g., indoor, outdoor) and (iii) the ability to scale to a large number of phones without jeopardizing the "phone experience" (e.g., usability and battery lifetime). Darwin is a collaborative reasoning framework built on three concepts: classifier/model evolution, model pooling, and collaborative inference. To the best of our knowledge Darwin is the first system that applies distributed machine learning techniques and collaborative inference concepts to mobile phones. We implement the Darwin system on the Nokia N97 and Apple iPhone. While Darwin represents a general framework applicable to a wide variety of emerging mobile sensing applications, we implement a speaker recognition application and an augmented reality application to evaluate the benefits of Darwin. We show experimental results from eight individuals carrying Nokia N97s and demonstrate that Darwin improves the reliability and scalability of the proof-of-concept speaker recognition application without additional burden to users.
Smooth pursuit eye movements and neuropsychological impairments in schizophrenia

Smooth pursuit eye movements (SPEM) and neuropsychological performance were examined in a sample of 29 drug‐treated schizophrenic patients and 22 healthy controls. Patients had impairment in SPEM as well as in a wide range of neuropsychological tests (Wechsler Adult Intelligence Scale, Wisconsin Card Sorting Test. Finger Tapping, Reaction time, Selective attention, Trail‐Making and Simultaneous Capacity). Performance indices were more affected than strategy‐executive indices. Drug type (clozapine vs typical) and dose (chlorpromazine units) were not related to neuropsychological impairment among the patients. Indices from the whole range of tests accurately predicted a subjects’ group identity (patient vs control). Impaired SPEM was predicted more accurately by tests assessing frontal functions. Seven patients, all men, had only marginal neuropsychological impairments.
Halofuginone inhibits TGF-β/BMP signaling and in combination with zoledronic acid enhances inhibition of breast cancer bone metastasis

More efficient therapies that target multiple molecular mechanisms are needed for the treatment of incurable bone metastases. Halofuginone is a plant alkaloid-derivative with antiangiogenic and antiproliferative effects. Here we demonstrate that halofuginone is an effective therapy for the treatment of bone metastases, through multiple actions that include inhibition of TGFβ and BMP-signaling. Halofuginone blocked TGF-β-signaling in MDA-MB-231 and PC3 cells showed by inhibition of TGF-β–induced Smad-reporter, phosphorylation of Smad-proteins, and expression of TGF-β-regulated metastatic genes. Halofuginone increased inhibitory Smad7-mRNA and reduced TGF-β-receptor II protein. Proline supplementation but not Smad7-knockdown reversed halofuginone-inhibition of TGF-β-signaling. Halofuginone also decreased BMP-signaling. Treatment of MDA-MB-231 and PC3 cells with halofuginone reduced the BMP-Smad-reporter (BRE)4, Smad1/5/8-phosphorylation and mRNA of the BMP-regulated gene Id-1. Halofuginone decreased immunostaining of phospho-Smad2/3 and phospho-Smad1/5/8 in cancer cells in vivo. Furthermore, halofuginone decreased tumor-take and growth of orthotopic-tumors. Mice with breast or prostate bone metastases treated with halofuginone had significantly less osteolysis than control mice. Combined treatment with halofuginone and zoledronic-acid significantly reduced osteolytic area more than either treatment alone. Thus, halofuginone reduces breast and prostate cancer bone metastases in mice and combined with treatment currently approved by the FDA is an effective treatment for this devastating complication of breast and prostate-cancer.
Short Hairpin RNA Silencing of PHD-2 Improves Neovascularization and Functional Outcomes in Diabetic Wounds and Ischemic Limbs

The transcription factor hypoxia-inducible factor 1-alpha (HIF-1α) is responsible for the downstream expression of over 60 genes that regulate cell survival and metabolism in hypoxic conditions as well as those that enhance angiogenesis to alleviate hypoxia. However, under normoxic conditions, HIF-1α is hydroxylated by prolyl hydroxylase 2, and subsequently degraded, with a biological half-life of less than five minutes. Here we investigated the therapeutic potential of inhibiting HIF-1α degradation through short hairpin RNA silencing of PHD-2 in the setting of diabetic wounds and limb ischemia. Treatment of diabetic mouse fibroblasts with shPHD-2 in vitro resulted in decreased levels of PHD-2 transcript demonstrated by qRT-PCR, higher levels of HIF-1α as measured by western blot, and higher expression of the downstream angiogenic genes SDF-1 and VEGFα, as measured by qRT-PCR. In vivo, shPHD-2 accelerated healing of full thickness excisional wounds in diabetic mice compared to shScr control, (14.33 ± 0.45 days vs. 19 ± 0.33 days) and was associated with an increased vascular density. Delivery of shPHD-2 also resulted in improved perfusion of ischemic hind limbs compared to shScr, prevention of distal digit tip necrosis, and increased survival of muscle tissue. Knockdown of PHD-2 through shRNA treatment has the potential to stimulate angiogenesis through overexpression of HIF-1α and upregulation of pro-angiogenic genes downstream of HIF-1α, and may represent a viable, non-viral approach to gene therapy for ischemia related applications.
17.6-Gbps universal filtered multi-carrier encoding of GaN blue LD for visible light communication

TO-can packed GaN LD connected with impedance-matched transmission-line circuit board enhances the UFMC additive 16-QAM OFDM bandwidth to enable 17.6-Gbps VLC at 4.4-bit/s/Hz spectral-usage efficiency with suppressed inter-OFDM-carrier interference and improved BER of 1.07×10−4.
Left-Corner Parsing and Psychological Plausibility

It is well known that even extremely limited centerembedding causes people to have difficulty in comprehension, but that left- and right-branching constructions produce no such effect. If the difficulty in comprehension is taken to be a result of processing load, as is widely assumed, then measuring the processing load induced by a parsing strategy on these constructions may help determine its plausibility as a psychological model. On this basis, it has been argued [AJ91, JL83] that by identifying processing load with space utilization, we can rule out both top-down and bottom-up parsing as viable candidates for the human sentence processing mechanism, and that left-corner parsing represents a plausible alternative.Examining their arguments in detail, we find difficulties with each presentation. In this paper we revise the argument and validate its central claim. In so doing, we discover that the key distinction between the parsing methods is not the form of prediction (top-down vs. bottom-up vs. left-corner), but rather the ability to instantiate the operation of composition.
Dynamics of the Flow Exchanges between Matrix and Conduits in Karstified Watersheds at Multiple Temporal Scales

The focus of this paper is to investigate the ability to assess the flow exchanges between the matrix and the conduits in two karstified watersheds (Aliou and Baget, Ariège, France) using the KarstMod modeling platform. The modeling is applied using hourly and daily time series. First, the flow dynamics between the conduit and the surrounding matrix are described on a rainfall event scale (i.e., a few days). The model allows us to describe a physical reality concerning the flow reversal between matrix and conduit when there is a significant rainfall event. Then, the long-term trends (i.e., inter-annual) in the matrix water level are evidenced using the moving average over shifting horizon method (MASH). The mean water level in the matrix dropped about 10% to 15% since the late 1960s. Also, the matrix recharge has been delayed from February in the late 1960s to April since the 1990s. Moreover, the contribution of the matrix in the total spring flow is estimated though mass balance. It is estimated that the annual matrix contribution in the total spring flow is about 3% and it can increase to up to 25% during periods with low rainfall.
On the performance of cloud radio access networks using Matérn hard-core point processes

In this paper, the performance of a cloud radio access network (CRAN) is analysed, which consists of multiple randomly distributed remote radio heads (RRHs) and a macro base station (MBS). Different from previous works on CRAN where Poisson Point Process (PPP) is used to model spatial distribution of RRHs, a more realistic Matern Hard-core point process (MHCPP) model is adopted in this work. To compare system performance of CRAN when different transmission strategies are used, two RRH selection schemes are adopted including 1) the best RRH selection (BRS) and 2) all RRHs participation (ARP). Considering downlink transmission, the outage probability and system throughput of CRAN are analytically characterized. The presented results demonstrate that compared to PPP model, the presence of hard-core distance will increase outage probability. Furthermore, the BRS scheme is more energy-efficient than the ARP scheme. Moreover, it is shown that the hard-core distance has a more significant impact on systems with higher intensity of PPP distributed candidate points and in large hard-core distance regime increasing the intensity of candidate points can only provide a small improvement in outage performance.
Modeling Age Progression in Young Faces

We propose a craniofacial growth model that characterizes growth related shape variations observed in human faces during formative years. The model draws inspiration from the ‘revised’ cardioidal strain transformation model proposed in psychophysical studies related to craniofacial growth. The model takes into account anthropometric evidences collected on facial growth and hence is in accordance with the observed growth patterns in human faces across years. We characterize facial growth by means of growth parameters defined over facial landmarks often used in anthropometric studies. We illustrate how the age-based anthropometric constraints on facial proportions translate into linear and non-linear constraints on facial growth parameters and propose methods to compute the optimal growth parameters. The proposed craniofacial growth model can be used to predict one’s appearance across years and to perform face recognition across age progression. This is demonstrated on a database of age separated face images of individuals under 18 years of age.
Impact of Atrial Fibrillation on Exercise Capacity in Heart Failure With Preserved Ejection Fraction: A RELAX Trial Ancillary Study

Background— Atrial fibrillation (AF) is common among patients with heart failure and preserved ejection fraction (HFpEF), but its clinical profile and impact on exercise capacity remain unclear. RELAX (Phosphodiesterase-5 Inhibition to Improve Clinical Status and Exercise Capacity in HFpEF) was a multicenter randomized trial testing the impact of sildenafil on peak VO2 in stable outpatients with chronic HFpEF. We sought to compare clinical features and exercise capacity among patients with HFpEF who were in sinus rhythm (SR) or AF. Methods and Results— RELAX enrolled 216 patients with HFpEF, of whom 79 (37%) were in AF, 124 (57%) in SR, and 13 in other rhythms. Participants underwent baseline cardiopulmonary exercise testing, echocardiogram, biomarker assessment, and rhythm status assessment before randomization. Patients with AF were older than those in SR but had similar symptom severity, comorbidities, and renal function. &bgr;-blocker use and chronotropic indices were also similar. Despite comparable left ventricular size and mass, AF was associated with worse systolic (lower EF, stroke volume, and cardiac index) and diastolic (shorter deceleration time and larger left atria) function compared with SR. Pulmonary artery systolic pressure was higher in AF. Patients with AF had higher N-terminal pro-B-type natriuretic peptide, aldosterone, endothelin-1, troponin I, and C-telopeptide for type I collagen levels, suggesting more severe neurohumoral activation, myocyte necrosis, and fibrosis. Peak VO2 was lower in AF, even after adjustment for age, sex, and chronotropic response, and VE/VCO2 was higher. Conclusions— AF identifies an HFpEF cohort with more advanced disease and significantly reduced exercise capacity. These data suggest that evaluation of the impact of different rate or rhythm control strategies on exercise tolerance in patients with HFpEF and AF is warranted. Clinical Trial Registration— URL: http://www.clinicaltrials. gov. Unique identifier: NCT00763867.
Case study: visual analysis of complex, time-dependent simulation results of a diesel exhaust system

In previous work we have presented visualization techniques that provide engineers with a high degree of interactivity and flexibility for analyzing large, time-dependent, and high-dimensional data sets resulting from CFD (computational fluid dynamics) simulations. In this case study we apply our techniques in the fields of the automotive engineering industry and demonstrate how users benefit from using them during their routine analysis, as well as for exploring new phenomena. For coping with some of the special requirements in this application, we adapted and extended parts of the system. A comparison of two related cases of a diesel exhaust system is presented, and some important questions about these cases are addressed.
Health of the nation.

used in terms of mmoUl. Mans! vears ago I was taught that the best oral rehydration solution was one that was made up of eight tablespoons of sugar and half a teaspoon of salt in one litre of water. Three glasses of this mixture were to be given after each stool. Does this simple, plain, and uncomplicated oral mixture, which could be understood by anyone, correspond to the glucose-electrolyte solution, measured in mmol/l, mentioned in the article?
Petteri Mannersalo Gaussian and multifractal processes in teletraffic theory

In this thesis, we consider two classes of stochastic models which both capture some of the essential properties of teletraffic. Teletraffic has two time regimes where profoundly different behavior and characteristics are seen. When traffic traces are observed at coarse resolutions, properties like self-similarity and longrange dependence are visible. In small time-scales, traffic exhibits complex scaling laws with much more spiky bursts than in coarser resolutions. The main part of the thesis is devoted to a large time-scale analysis by considering Gaussian processes and queueing systems with Gaussian input. In order to understand the small time-scale dynamics, first steps are taken towards general multifractal models offering a suitable basis for short time-scale teletraffic modeling. The family of Gaussian processes with stationary increments serves as the traffic model for large time-scales. First, we introduce a fast and accurate simulation algorithm, which can be used to generate long approximate Gaussian traces. Moreover, the algorithm is also modified to run on-the-fly. Then approximate queue length distributions for ordinary, priority and generalized processor sharing queues are derived using a most probable path approach. Simulation studies show that the performance formulae appear to be quite accurate over the full range of buffer levels. Finally, we construct a semi-stationary predictor, which uses a constant variance function and mean rate estimation based on a moving average method. Moreover, we show that measuring the past of a process by geometrically increasing intervals is a good engineering solution and a much better way than equally spaced measurements. We introduce a family of multifractal processes which belongs to the framework of T-martingales and multiplicative chaos introduced by Kahane. The family has many desirable properties like stationarity of increments, concave multifractal spectra and simple construction. We derive, for example, conditions for nondegeneracy, establish a power law for the moments and obtain a formula for the multifractal spectrum.