Miracle-dz/UniFAR-FTUs huggingface dataset:
SARS-CoV-2-Specific CD8+ T-Cells in Blood but Not in the Lungs of Vaccinated K18-hACE2 Mice after Infection

Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 enters the host by infecting nasal ciliated cells. Then, the virus can spread towards the oropharyngeal cavity and the pulmonary tissues. The antiviral adaptive immunity is promptly induced in response to the virus’s detection, with virus-specific T-lymphocytes appearing before antiviral antibodies. Both the breadth and potency of antiviral CD8+ T-cell immunity have a key role in containing viral spread and disease severity. Current anti-SARS-CoV-2 vaccines do not impede the virus’s replication in the upper respiratory tract, and there is consensus on the fact that the best potency of the antiviral immune response in both blood and the upper respiratory tract can be reached upon infection in vaccinees (i.e., breakthrough infection). However, whether the antiviral CD8+ T-cells developing in response to the breakthrough infection in the upper respiratory tract diffuse to the lungs is also still largely unknown. To fill the gap, we checked the CD8+ T-cell immunity elicited after infection of K18-hACE2 transgenic mice both at 3 weeks and 3 months after anti-spike vaccination. Virus-specific CD8+ T-cell immunity was monitored in both blood and the lungs before and after infection. By investigating the de novo generation of the CD8+ T-cells specific for SARS-CoV-2 viral proteins, we found that both membrane (M) and/or nucleocapsid (N)-specific CD8+ T-cells were induced at comparable levels in the blood of both unvaccinated and vaccinated mice. Conversely, N-specific CD8+ T-cells were readily found in the lungs of the control mice but were either rare or absent in those of vaccinated mice. These results support the idea that the hybrid cell immunity developing after asymptomatic/mild breakthrough infection strengthens the antiviral cell immunity in the lungs only marginally, implying that the direct exposition of viral antigens is required for the induction of an efficient antiviral cell immunity in the lungs.
Genotypic differences in root architecture and physiological characteristics in mango (Mangifera indica) under drought

An experiment was conducted at the research farm of ICAR-Indian Agricultural Research Institute, New Delhi during 2020 and 2021 to study the genotypic differences in root architecture and physiological characteristics in mango (Mangifera indica L.) under drought. One-year-old polyembryonic 7 mango rootstock genotypes were exposed to normal irrigation and drought conditions for 24 days. The drought-induced increased total plant weight and dry weight in Kurukkan. The shoot dry weight decreased in all genotypes ranging from 5.13% in OLP-Z-6/1 to 81.82% in Kurukkan. There was a lesser reduction in membrane stability index in Kurukkan and K-5, stomata count in Kurukkan, Olour and OPK-3-7/12 at the end of the drought period. Root surface area increased under drought in Kurukkan, while it decreased more in K-5. Correlation studies confirmed a strong positive relationship between relative water content (RWC) and membrane stability index (MSI), number of stomata (SC), number of leaves (NL), root tips (RT) and root forks (NRF); MSI and number of root tips (NRT); dry weight of the whole plant and root surface area (RSA); the dry weight of roots and root volume (RV), RSA, and plant height (Ph); root length (RL) and RSA, RT, and NRF. Kurukkan was identified as drought-tolerant based on a higher SC, RWC, RV, RSA, dry mass, NRT and NRF. Results indicated that the root indices offer a promising strategy for the screening of drought-tolerant mango genotypes.
Study of the diatoms of Reghaia Lake, northern Algeria

En raison de leur importante capacite de reponse aux changements environnementaux, les diatomees sont depuis longtemps utilisees comme indicateurs biologiques de la qualite des milieux aquatiques. Cette flore a ete tres peu etudiee en Algerie et en Afrique du Nord ; pour cela l’objectif de cette etude est de decrire les variabilites spatio-temporelles des diatomees a l’echelle du lac de Reghaia, qui se deverse dans la Mediterranee, et les principaux facteurs abiotiques responsables de ces variabilites. D’octobre 2007 a septembre 2008, des etudes hydrobiologiques ont ete entreprises dans ce lac. L'evolution mensuelle des parametres physico-chimiques permet de classer cette eau dans la qualite mediocre. Les elements nutritifs (PO4 et NH4), la temperature et l’oxygene marquent des fluctuations importantes et influent directement sur la repartition des diatomees. Nos resultats ont montre que les communautes diatomiques planctoniques recoltees se composent de 24 especes, dont 10 ont ete signalees pour la premiere fois en Algerie. L’espece Cyclotella ocellata est la plus abondante avec des frequences mensuelles qui varient de 4,7 % de la communaute diatomique totale (au mois de mars) a 97 % (au mois d’aout). Quant a la variabilite spatiale, les stations de l’amont sont caracterisees par la dominance des especes polluo-resistantes, ce qui est le temoin de la forte charge polluante affectant ces zones. Ces resultats montrent que les assemblages de diatomees sont influences par les perturbations anthropiques et suggerent qu’ils peuvent etre utilises comme indicateurs de la qualite de l’environnement
JD-QTZ-BEARING METAPLAGIOGRANITE FROM THE MONVISO META-OPHIOLITE (WESTERN ALPS)

In the Basal Serpentinite Unit of the Monviso meta-ophiolite (Val Varaita, Western Alps), a large jadeite-quartz-bearing metaplagiogranite body has been found, which shows magmatic contacts with eclogitic Pl-Cpx-FeTi oxide metagabbro. Primary texture and magmatic minerals in the metaplagiogranite are mostly obliterated by multistage Alpine re-equilibrations. The highest-pressure assemblage consisted of jadeite, quartz, phengite and rutile. Garnet and zoned ferroglaucophane developed immediately after the peak assemblage, and were followed by aegirine-rich clinopyroxene. Calculated phase compatibilities and phengite geobarometry indicate T = 575°C and P = 1.95 GPa as minimal temperature and pressure of formation for the peak assemblage in the metaplagiogranite, in agreement with garnet-clinopyroxene geothermometry from the associated FeTi-oxide metagabbro which yields T = 545±35°C at P = 2.0 GPa. Major, trace, and REE data are typical of oceanic plagiogranite, and similar to those of metaplagiogranite from other meta-ophiolite units of the Western Alps, Northern Apennines and Corsica. Petrological and preliminary geochemical data suggest that the Monviso plagiogranite likely represents late stages of the plutonic activity in this sector of the Western Tethys, and could have formed by fractional crystallization of ocean-floor tholeiite magmas in Late Jurassic times.
Multimode microwave circuit optomechanics as a platform to study coupled quantum harmonic oscillators

Harmonic oscillators might be one of the most fundamental entities described by physics. Yet they stay relevant in recent research. The topological properties associated with exceptional points that can occur when two modes interact have generated much interest in recent years. Harmonic oscillators are also at the heart of new quantum technological applications: the long lifetime of high-Q resonators make them advantageous as quantum memories, and they are employed for narrowband processing of quantum signals, as in Josephson parametric amplifiers. The goal of this thesis is to explore different fundamental regimes of two coupled harmonic oscillators using cavity optomechanics as the experimen- tal platform. With consistent progress in attaining ever increasing Q factors, mechanical and electromagnetic resonators realize near-ideal harmonic oscillators. By parametrically modulating the nonlinear optomechanical interaction between them, an effective linear coupling is achieved, which is tunable in strength and in the relative frequencies of the two modes. Thus cavity optomechanics provides a framework with excellent control over system parameters for the study of two coupled harmonic modes. The specific optomechanical implementation employed are superconducting circuits with the vibrating top plate of a capacitor as the mechanical element. Multimode optomechanical circuits are realized, with two microwave modes interacting with one or two mechanical oscillators. The supplementary modes serve either as intermediaries in the relation of the two modes of interest, or as auxiliaries used to control a parameter of the system. Three main experimental results are achieved. First, an auxiliary microwave mode allows the engineering of the effective dissipation rate of a mechanical oscillator. The latter then acts as a reservoir for the main microwave mode with which it interacts. The microwave mode susceptibility can be tuned, resulting in an instability akin to that of a maser and in resonant amplification of incoming microwave signals with an added noise close to the quantum minimum. Second, we study the conditions for a nonreciprocal interaction between two microwave modes, when the information flows in one direction but not in the other. The two modes interact through two mechanical oscillators, leading to frequency conversion between the two cavities. Dissipation in the mechanical modes is essential to the scheme in two ways: it provides a reciprocal phase necessary for the interference and eliminates the unwanted signals. Third, level attraction between a microwave and a mechanical mode is demonstrated, where the eigenfrequencies of the system are drawn closer as the result of interaction, rather distancing themselves as in the more usual case of level repulsion. The phenomenon is theoretically connected to exceptional points, and a general classification of the possible regimes of interaction between two harmonic modes is exposed, including level repulsion and attraction as special cases.
Dissemination of Class A Cephalosporinases and Class D Carbapenemases in Escherichia coli Isolates From a Tertiary Hospital in Sudan

Introduction The high prevalence of urinary tract infections (UTIs) and rising resistance to beta-lactam antibiotics, which is a global therapeutic concern, are caused by Escherichia coli (E. coli) extended-spectrum beta-lactamases (ESBLs) producers. It is unclear how E. coli that produces ESBLs spreads throughout Gezira state, Sudan. The study aimed to evaluate the dissemination of class A and class D resistance genes among E. coli and to recognize the antibacterial activity of the locally used cephalosporins and carbapenems. Methods One hundred and fifteen isolates of uropathogenic E. coli were collected from patients who attended a tertiary hospital. The isolates were identified using colony morphology, gram staining, and biochemical tests and checked for 16S rRNA using PCR. The multidrug-resistant (MDR) testing was conducted using agar disk diffusion. Finally, the class A and D resistance genes were analyzed by multiplex PCR. Results The study enrolled 200 patients with UTIs. E. coli isolates were found in 115 (57.5%) urine specimens examined, and 60 (52.2%) of them produced resistance to most locally used antibiotics. The antibiotic resistance pattern was higher against cefepime (100%), ceftizoxime (90%), cefuroxime (81.7%), and ceftriaxone (81.7%) and had lower activity against meropenem (13.3%). The genotypic characterization of class A cephalosporinases was 85% for blaCTX-M, 70% for blaSHV, and 33.3% for blaTEM, while for class D carbapenemases, it was 10% for both blaOXA-23 and blaOXA-51. Conclusion The considerable antibiotic resistance to the cephalosporins and meropenem and the increased predominance of the blaCTX-M and blaSHV genes are serious concerns for the health authorities. Meropenem could still be used as the drug of choice for ESBL-producing E. coli.
Novel Double Hybrid-Type Bone Cements Based on Calcium Phosphates, Chitosan and Citrus Pectin

In this work, the influence of the liquid phase composition on the physicochemical properties of double hybrid-type bone substitutes was investigated. The solid phase of obtained biomicroconcretes was composed of highly reactive α-tricalcium phosphate powder (α-TCP) and hybrid hydroxyapatite/chitosan granules (HA/CTS). Various combinations of disodium phosphate (Na2HPO4) solution and citrus pectin gel were used as liquid phases. The novelty of this study is the development of double-hybrid materials with a dual setting system. The double hybrid phenomenon is due to the interactions between polycationic polymer (chitosan in hybrid granules) and polyanionic polymer (citrus pectin). The chemical and phase composition (FTIR, XRD), setting times (Gillmore needles), injectability, mechanical strength, microstructure (SEM) and chemical stability in vitro were studied. The setting times of obtained materials ranged from 4.5 to 30.5 min for initial and from 7.5 to 55.5 min for final setting times. The compressive strength varied from 5.75 to 13.24 MPa. By incorporating citrus pectin into the liquid phase of the materials, not only did it enhance their physicochemical properties, but it also resulted in the development of fully injectable materials featuring a dual setting system. It has been shown that the properties of materials can be controlled by using the appropriate ratio of citrus pectin in the liquid phase.
Boron-induced microstructural manipulation of titanium and titanium alloys in additive manufacturing

ABSTRACT While the role of boron (B) has been thoroughly clarified in titanium (Ti) castings, the microstructural changes triggered in additive manufacturing (AM) are still the subject of debate in the literature. Many contributions have confirmed the B-induced microstructural refinement in Ti-based AM parts. The formation of TiB in titanium matrix composites (TMCs) may increase strength. In some cases, B may also promote the columnar-to-equiaxed transition, thus mitigating the anisotropic effects associated with the strong epitaxial growth of unidirectional columnar grains typical of AM. However, as critically discussed in this review, some pitfalls remain. Due to fast cooling, the microstructural evolution in AM may deviate from equilibrium, leading to a shift of the Ti-B eutectic point and to the formation of out-of-equilibrium phases. Additionally, the growth of TiB may undermine the ductility and the crack propagation resistance of AM parts, which calls for appropriate remediation strategies.
Deciphering the binding mechanism of an anti-cancer phytochemical plumbagin with calf thymus DNA using biophysical and in silico techniques

Plumbagin (PLM), a plant derivative, is well known for a wide range of therapeutic effects in humans including anti-cancer, anti-inflammatory, anti-oxidant, and anti-microbial. Cytotoxic and genotoxic potential of this phytochemical has been studied which demands further insight. DNA being a major target for several drugs was taken to study against PLM to understand its effects on the cellular system. UV-Vis spectroscopy has indicated the binding of PLM to ctDNA and dye displacement assays have confirmed the formation of PLM-ctDNA complex. The insignificant changes in circular dichroism spectra suggested that PLM is not affecting the structural makeup of the ctDNA, hence the binding could be peripheral and not intercalating. Further, the relative viscosity and minimal change in melting temperature upon the complex formation supported this finding and confirmed the groove binding of PLM. Molecular docking analysis and simulation studies also show PLM as a minor groove binder to DNA and provide details on the interaction dynamics of PLM-DNA complex. Docking followed by a 100 ns simulation reveals the negative Gibbs free energy change (∆G = −6.6 kcal mol−1), and the formation of a stable complex. The PLM- DNA complex with stable dynamics was further supported by different parameters including RMSD, RMSF, SASA, Rg, and the energy profile of interaction. This study provides an insight into the cytotoxic and genotoxic mechanism of PLM which can be a crucial step forward to exploit its therapeutic potential against several diseases including cancer.
B-Type Fumonisins in Post-Fermented Tea: Occurrence and Consumer Dietary Exposure in Guangxi, China

Post-fermented tea (PFT), a commonly consumed beverage worldwide, is characterized by the rapid growth of its microbial groups and the substantial changes they undergo. Consequently, PFT may contain mycotoxins such as B-type fumonisins (FBs). This study aimed to assess the intake of FBs through the consumption of PFT among consumers in Guangxi, China. A novel quantitative method using high-performance liquid chromatography-mass spectrometry was used to determine the FB concentration in PFT products. Additionally, a PFT consumption survey was conducted using a face-to-face questionnaire, recording their body weight and PFT consumption patterns based on a three-day dietary recall method. Finally, hazard index was calculated to estimate the health risk of FBs from the consumption of PFT products in Guangxi. The results revealed that the occurrence of FBs in PFT was 20% (24/120), with a concentration ranging from 2.14 to 18.28 μg/kg. The results of the survey showed that the average daily consumption of PFT by consumers was 9.19 ± 11.14 g. The deterministic risk assessment revealed that only 0.026% of the provisional maximum tolerable daily intake of FBs was consumed through PFT, indicating that FB contamination in PFT is not a public health risk.
Comparative analysis of accuracy between fine-needle aspiration biopsy and postoperative histopathology for detecting large thyroid nodules: A retrospective observational study

To avoid unnecessary surgeries, ultrasound-guided fine-needle aspiration biopsy (FNAB) is an effective and reliable procedure for the preoperative evaluation of thyroid nodules. However, there have been only a limited number of studies exploring the ability of preoperative FNAB to distinguish malignancy compared to postoperative histopathology in thyroid nodules larger than 4 cm. The aim of this study was to investigate the diagnostic accuracy of FNAB compared to postoperative histopathology in distinguishing malignancy in thyroid nodules larger than 4 cm. A single-center retrospective observational study was conducted at Dr. Zainoel Abidin Hospital, Banda Aceh, Indonesia, between January 2014 and December 2018. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were calculated. A total of 83 patients were included in the study. The results showed that preoperative FNAB may have the ability to distinguish malignancy compared to postoperative histopathology. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were 42.85%, 98.38%, 90.00%, 83.56%, and 84.33%, respectively. These data suggested that ultrasound-guided preoperative FNAB is a reliable diagnostic tool in the preoperative evaluation of thyroid nodules larger than 4 cm, but it has limited capability in distinguishing malignancies. In conclusion, although FNAB may be useful in reducing unnecessary surgeries, histopathology remains the preferred method for confirming malignancy in thyroid nodules.
Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays

Epilepsy severely impairs the cognitive behavior of patients. It remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex (MEC), a region known for its involvement in spatial cognition. To explore this neural mechanism, we recorded the spikes and local field potentials from MEC neurons in lithium–pilocarpine-induced epileptic rats using self-designed microelectrode arrays. Through the open field test, we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of epilepsy. Meanwhile, we analyzed theta oscillations and theta modulation in both excitatory and inhibitory neurons. Furthermore, we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic rats. After the epilepsy modeling, the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period (1 to 5 d). Subsequently, the firing characteristics and theta oscillations were impaired. In the chronic period (>10 d), the performance in the novel object experiment deteriorated. In conclusion, our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency, suggesting the potential use of these changes as a “functional biomarker” for predicting cognitive impairment caused by epilepsy.
The Influence of Perceived Effective Sanctions on Customer’s Initial Trusting Beliefs and Initial Trusting Intention towards Unfamiliar Web Retailers

Past studies have conceptualized initial trust in diverse ways. Although many have examined the effect of perceived effective sanctions on initial trust, the outcomes are still unclear, and more aspects remain untapped. As such, this study assesses the effect of perceived effective sanctions on customers’ initial trusting beliefs and initial trusting intentions towards unfamiliar web retailers. Data collected from a survey were statistically analyzed using the Structural Equation Modelling (SEM) technique. As a result, perceived effective sanctions displayed a significantly positive effect on both initial trusting beliefs and intention. This study enhances the understanding of initial trust and its association with perceived effective sanctions. It also offers further understanding of the effect of the construct on initial trust within the under-researched area in Malaysia. Practically, this study facilitates less reputable web retailers in devising effective strategies to build initial trust amongst consumers.