Miracle-dz/UniFAR-FTUs huggingface dataset:
Individual differences in sadness coherence: Associations with dispositional affect and age.

The current laboratory-based study examined individual differences in sadness coherence (i.e., coherence between objectively coded sad facial expressions and heart rate in response to a sad film clip) and associations with dispositional affect (i.e., positive and negative affect, extraversion, neuroticism) and age in a sample of younger and older adults. Results showed that (a) greater sadness coherence was associated with lower dispositional negative affect (i.e., greater positive to negative affect ratio; lower neuroticism) and (b) older adults had greater sadness coherence than younger adults. Findings remained stable when controlling for covariates. Results were specific to coherence characterized by an inverse association between heart rate and facial expressions of sadness (i.e., did not emerge for absolute changes in heart rate or skin conductance), specific to sad facial expressions (i.e., did not emerge for happy facial expressions), specific to stimulus (i.e., did not emerge for sadness coherence in response to a happy film clip), generalized across overall levels of emotional responding (i.e., sad facial expressions; heart rate reactivity), and remained stable when controlling for expressive suppression. These findings demonstrate that individuals who exhibit greater sadness coherence experience more favorable dispositional affect, consistent with evolutionary-functionalist models of emotion, and that sadness coherence is higher in late life, consistent with developmental accounts of heightened reactivity to loss in late life. (PsycINFO Database Record (c) 2020 APA, all rights reserved).
Bariatric Surgery in Patients With Obesity and Latent Autoimmune Diabetes in Adults (LADA)

Bariatric surgery induces weight loss and restores glucose metabolism in patients with obesity and type 2 diabetes (T2D) (1). In patients with autoimmune diabetes that eventually results in β-cell failure, the glycemic effects of bariatric surgery are limited (2). Adult patients with latent autoimmune diabetes in adults (LADA) have a slower decline in β-cell function and lower requirement for insulin therapy than patients with classical young-onset type 1 diabetes (T1D) (3). They are often misdiagnosed as having T2D and might have unrealistic expectations of diabetes remission after bariatric surgery with additional risk for development of postoperative diabetic ketoacidosis (DKA) (4). The safety and efficacy of bariatric surgery in patients with obesity and LADA have not been characterized. We retrospectively analyzed medical records of patients with obesity and LADA who underwent bariatric surgery at the Cleveland Clinic Health System in the U.S. LADA status was defined as adult-onset diabetes with presence of GAD antibodies. We compared pre- and postsurgery BMI, glycemic control (HbA1c), daily insulin dose, and blood pressure and lipid profile using a paired Student t test, with a P value of 0.05 considered significant. Between October 2006 and March 2018, nine female patients underwent laparoscopic bariatric surgery (five Roux-en-Y gastric bypass and four sleeve gastrectomy) and one male patient underwent sleeve gastrectomy. The median age at …
The determinants of maternal homocysteine in pregnancy: findings from the Ottawa and Kingston Birth Cohort

Abstract Objective: Observational studies have linked elevated homocysteine to vascular conditions. Folate intake has been associated with lower homocysteine concentration, although randomised controlled trials of folic acid supplementation to decrease the incidence of vascular conditions have been inconclusive. We investigated determinants of maternal homocysteine during pregnancy, particularly in a folic acid-fortified population. Design: Data were from the Ottawa and Kingston Birth Cohort of 8085 participants. We used multivariable regression analyses to identify factors associated with maternal homocysteine, adjusted for gestational age at bloodwork. Continuous factors were modelled using restricted cubic splines. A subgroup analysis examined the modifying effect of MTHFR 677C>T genotype on folate, in determining homocysteine concentration. Setting: Participants were recruited in Ottawa and Kingston, Canada, from 2002 to 2009. Participants: Women were recruited when presenting for prenatal care in the early second trimester. Results: In 7587 participants, factors significantly associated with higher homocysteine concentration were nulliparous, smoking and chronic hypertension, while factors significantly associated with lower homocysteine concentration were non-Caucasian race, history of a placenta-mediated complication and folic acid supplementation. Maternal age and BMI demonstrated U-shaped associations. Folic acid supplementation of >1 mg/d during pregnancy did not substantially increase folate concentration. In the subgroup analysis, MTHFR 677C>T modified the effect of folate status on homocysteine concentration. Conclusions: We identified determinants of maternal homocysteine relevant to the lowering of homocysteine in the post-folic acid fortification era, characterised by folate-replete populations. A focus on periconceptional folic acid supplementation and improving health status may form an effective approach to lower homocysteine.
The RNA-seq transcriptomic analysis reveals genes mediating salt tolerance through rapid triggering of ion transporters in a mutant barley

Considering the complex nature of salinity tolerance mechanisms, the use of isogenic lines or mutants possessing the same genetic background albeit different tolerance to salinity is a suitable method for reduction of analytical complexity to study these mechanisms. In the present study, whole transcriptome analysis was evaluated using RNA-seq method between a salt-tolerant mutant line “M4-73-30” and its wild-type “Zarjou” cultivar at seedling stage after six hours of exposure to salt stress (300 mM NaCl). Transcriptome sequencing yielded 20 million reads for each genotype. A total number of 7116 transcripts with differential expression were identified, 1586 and 1479 of which were obtained with significantly increased expression in the mutant and the wild-type, respectively. In addition, the families of WRKY, ERF, AP2/EREBP, NAC, CTR/DRE, AP2/ERF, MAD, MIKC, HSF, and bZIP were identified as the important transcription factors with specific expression in the mutant genotype. The RNA-seq results were confirmed at several time points using qRT-PCR for some important salt-responsive genes. In general, the results revealed that the mutant accumulated higher levels of sodium ion in the root and decreased its transfer to the shoot. Also, the mutant increased the amount of potassium ion leading to the maintenance a high ratio [K+]/[Na+] in the shoot compared to its wild-type via fast stomata closure and consequently transpiration reduction under the salt stress. Moreover, a reduction in photosynthesis and respiration was observed in the mutant, resulting in utilization of the stored energy and the carbon for maintaining the plant tissues, which is considered as a mechanism of salt tolerance in plants. Up-regulation of catalase, peroxidase, and ascorbate peroxidase genes has resulted in higher accumulation of H2O2 in the wild-type compared to the mutant. Therefore, the wild-type initiated rapid ROS signals which led to less oxidative scavenging in comparison with the mutant. The mutant increased expression in the ion transporters and the channels related to the salinity to maintain the ion homeostasis. In overall, the results demonstrated that the mutant responded better to the salt stress under both osmotic and ionic stress phases and lower damage was observed in the mutant compared to its wild-type under the salt stress.
High‐throughput analysis revealed the unique immunoglobulin gene rearrangements in plasmacytoma‐like post‐transplant lymphoproliferative disorder

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Transition of care of stable ischaemic heart disease patients from tertiary to primary care with telemedicine support: Randomized noninferiority clinical trial

Introduction Evidence of telehealth for chronic disease management is scarce and contradictory. Objectives We aimed to evaluate the safety and efficacy of teleconsultations as support in the care transition of patients with stable coronary artery disease (CAD) from tertiary to primary care. Methods A randomized noninferiority clinical trial was undertaken in patients with CAD from a tertiary hospital in a middle-income country. Patients with functional angina class 1 or 2 and meeting discharge criteria were randomized to remain in the cardiology outpatient clinic for 12 months (control group, CG) or continue follow-up in a primary care unit with clinical support via telemedicine (intervention group, IG). The primary outcome was the maintenance of the functional angina class after 12 months. Secondary outcomes included control of risk factors and clinical outcomes. Results In total 271 patients (mean age, 66 years) were included; 81.1% and 91% of the IG and CG, respectively, maintained stable angina symptoms, thus noninferiority could not be shown between the groups. Regarding emergency room visits at 1 year, the IG (7.6%) was noninferior to the CG (6.0%) (absolute difference, 1.6%; noninferiority margin (NIM), –4.8% to 8.2%). For control of risk factors, 30.7% and 29.6% of the IG and CG, respectively, had blood pressure <130/80 mmHg (absolute difference, 1.1%; NIM, –10.5% to 12.8%), and 48.9% and 33.3% of diabetic patients in the IG and CG, respectively, had glycated haemoglobin <7% (absolute difference, 15.6%; NIM, –6.8% to 36%). Conclusions In our study, the difference in the patients’ angina functional class did not result in greater seeking of emergency care, supporting that discharge from the outpatient clinic with telemedicine is safe for patients with stable CAD treated at the tertiary level. The control of risk factors in these patients was noninferior to patients followed up in primary care. ClinicalTrials.gov (NCT02489565).
Changes in renal function after switching from TDF to TAF in HIV-infected individuals: a prospective cohort study.

BACKGROUND Replacing tenofovir disoproxil fumarate (TDF) with tenofovir alafenamide (TAF) improves renal tubular markers in HIV-infected individuals, but the impact on estimated glomerular filtration rate (eGFR) remains unclear. METHODS We included all participants from the Swiss HIV Cohort Study who switched from a TDF to a TAF-containing antiretroviral regimen or continued TDF. We estimated changes in eGFR and urine protein-to-creatinine ratio (UPCR) after 18 months using mixed-effect models. RESULTS Of 3'520 participants (26.6% women, median age 50 years), 2'404 (68.5%) switched to TAF. Prior to switch, 1'664 (47.3%) had an eGFR <90 mL/min, and 1'087 (30.9%) a UPCR ≥15 mg/mmol. In patients with a baseline eGFR ≥90 mL/min, eGFR decreased with the use of TDF and TAF (-1.7 ml/min). Switching to TAF was associated with increases in eGFR of 1.5 mL/min (95% CI 0.5-2.5) if the baseline eGFR was 60-89 mL/min, and 4.1 mL/min (95% CI 1.6-6.6) if <60 mL/min. In contrast, eGFR decreased by 5.8 mL/min (95% CI 2.3-9.3) with the continued use of TDF in individuals with a baseline eGFR <60 mL/min. UPCR decreased after replacing TDF by TAF, independent of baseline eGFR. CONCLUSIONS Switching from TDF to TAF improves eGFR and proteinuria in patients with renal dysfunction.
Time course regulatory analysis based on paired expression and chromatin accessibility data

A time course experiment is a widely used design in the study of cellular processes such as differentiation or response to stimuli. In this paper, we propose time course regulatory analysis (TimeReg) as a method for the analysis of gene regulatory networks based on paired gene expression and chromatin accessibility data from a time course. TimeReg can be used to prioritize regulatory elements, to extract core regulatory modules at each time point, to identify key regulators driving changes of the cellular state, and to causally connect the modules across different time points. We applied the method to analyze paired chromatin accessibility and gene expression data from a retinoic acid (RA)–induced mouse embryonic stem cells (mESCs) differentiation experiment. The analysis identified 57,048 novel regulatory elements regulating cerebellar development, synapse assembly, and hindbrain morphogenesis, which substantially extended our knowledge of cis-regulatory elements during differentiation. Using single-cell RNA-seq data, we showed that the core regulatory modules can reflect the properties of different subpopulations of cells. Finally, the driver regulators are shown to be important in clarifying the relations between modules across adjacent time points. As a second example, our method on Ascl1-induced direct reprogramming from fibroblast to neuron time course data identified Id1/2 as driver regulators of early stage of reprogramming.
The Clinical Characteristics of Fractures in Pediatric Patients Exposed to Proton Pump Inhibitors.

OBJECTIVES There are increasing concerns regarding proton pump inhibitor (PPI) use and the risk of fractures in adults. Few studies have evaluated this risk among pediatric patients. This study examined fractures and fracture location among pediatric patients exposed to PPI compared with those without documented exposure. STUDY DESIGN Encounters for patients 6 months to 15.5 years were identified between July 1, 2011 to December 31, 2015 in the Pediatric Hospital Information System database. Exclusion criteria was applied for chronic illnesses, conditions or medications predisposing to fracture. Encounters were classified as PPI encounters if a charge for PPI was documented. PPI encounters were propensity matched to non-PPI encounters. Following initial encounter, patients were evaluated over a 2-year period for hospitalizations resulting from fracture. RESULTS There was a statistically significant higher rate of fractures among the PPI-exposed group (1.4% vs 1.2%, P = 0.019). Adjusting for remaining differences in sex, race, encounter type, payer, and resource intensity after matching, the difference remained statistically significant (P = 0.017) with an adjusted odds ratio (95% CI) of 1.2 (1.0--1.4). Upper extremity was the most common location for fracture; however, the PPI cohort was more likely to suffer from lower extremity, rib, and spinal fractures (P = 0.01). CONCLUSIONS This study suggests an increased risk of fracture among pediatric patients taking PPI. Among patients hospitalized with a fracture, those with PPI exposure had a higher rate of lower extremity, rib, and spine fractures compared with controls. This appeared to be a class effect not related to individual PPI agent.
Combined maternal exposure to cypermethrin and stress affect embryonic brain and placental outcomes in mice.

Prenatal exposure to cypermethrin is a risk factor for adverse neurodevelopmental outcomes in children. In addition, maternal psychological stress during pregnancy has significant effects on fetal neurodevelopment and may influence end-stage toxicity to offspring by altering maternal xenobiotic metabolism. As such, this study examined effects of maternal exposure to alpha-cypermethrin and stress, alone and in combination, on offspring development; with a focus on fetal neurotoxicity. CD1 mouse dams were administered 10mg/kg alpha-cypermethrin or corn oil vehicle via oral gavage from E11-E14. In addition, dams from each treatment were subjected to a standard model of restraint stress from E12-E14. Cypermethrin treatment impaired fetal growth, reduced fetal forebrain volume, and increased ventral forebrain proliferative zone volume, the latter effects driven by combined exposure with stress. Cypermethrin also impaired migration of GABAergic progenitors, with different transcriptional changes alone and in combination with stress. Stress and cypermethrin also interacted in effects on embryonic microglia morphology. In addition, levels of cypermethrin were elevated in the serum of stressed dams, which was accompanied by interacting effects of cypermethrin and stress on hepatic expression of cytochrome p450 enzymes. Levels of cypermethrin in amniotic fluid were below the limit of quantitation, suggesting minimal transfer to fetal circulation. Despite this, cypermethrin increased placental malondialdehyde levels and increased placental expression of genes responsive to oxidative stress, effects significantly modified by stress exposure. These findings suggest a role for interaction between maternal exposures to cypermethrin and stress on offspring neurodevelopment, involving indirect mechanisms in the placenta and maternal liver.
The influence of reward magnitude on stimulus memory and stimulus generalization in categorization decisions.

Reward magnitude is a central concept in most theories of preferential decision making and learning. However, it is unknown whether variable rewards also influence cognitive processes when learning how to make accurate decisions (e.g., sorting healthy and unhealthy food differing in appeal). To test this, we conducted 3 studies. Participants learned to classify objects with 3 feature dimensions into two categories before solving a transfer task with novel objects. During learning, we rewarded all correct decisions, but specific category exemplars yielded a 10 times higher reward (high vs. low). Counterintuitively, categorization performance did not increase for high-reward stimuli, compared with an equal-reward baseline condition. Instead, performance decreased reliably for low-reward stimuli. To analyze the influence of reward magnitude on category generalization, we implemented an exemplar-categorization model and a cue-weighting model using a Bayesian modeling approach. We tested whether reward magnitude affects (a) the availability of exemplars in memory, (b) their psychological similarity to the stimulus, or (c) attention to stimulus features. In all studies, the evidence favored the hypothesis that reward magnitude affects the similarity gradients of high-reward exemplars compared with the equal-reward baseline. The results from additional reward-judgment tasks (Studies 2 and 3) strongly suggest that the cognitive processes of reward-value generalization parallel those of category generalization. Overall, the studies provide insights highlighting the need for integrating reward- and category-learning theories. (PsycINFO Database Record (c) 2020 APA, all rights reserved).
Evaluation of diode laser application on chemical analysis and surface microhardness of white spots enamel lesions with two remineralizing agents

Background To investigate the effect of diode laser application and two commercial remineralizing agents on the remineralization and surface microhardness of white spot enamel lesions. Material and Methods Sixty specimens were prepared then equally divided into six groups (n=10/group), according to the diode laser and the two commercial remineralizing agents applied to demineralized enamel surfaces (APF gel and sodium fluoride NaF mousse) with or without diode Laser application as follows; Group A; control, Group B; diode Laser application, Group C; APF gel application, Group D; NaF mousse application, Group E; APF gel application + diode Laser, Group F; NaF mousse application+ diode Laser. Then the teeth were investigated for their Ca, P & F ions content and surface microhardness. One-way ANOVA followed by Tukey’s (HSD) post hoc test were used for statistical analysis. Results Ca ion wt% showed no statistically significant difference between tested groups, with the highest mean value recorded with Group C. P ion wt%, showed a statistically significant difference between Groups A and C, and the highest mean value was recorded for Group A. The highest F ion wt% was recorded for Group C, while the lowest was recorded for both A and B groups. The highest significant microhardness mean values was recorded for Group E, while the lowest was recorded for Group A. Conclusions Diode Laser treatment of the demineralized enamel surface had a positive influence on the chemistry and surface microhardness and it may represent a promising adjunct for enamel surface remineralization. Key words:Diode Laser application, chemical analysis, surface microhardness, remineralizing agents, white spot lesions.