Miracle-dz/UniFAR-FTUs huggingface dataset:
Increased placental mitochondrial fusion in gestational diabetes mellitus: an adaptive mechanism to optimize feto-placental metabolic homeostasis?

Introduction Gestational diabetes mellitus (GDM), a common pregnancy disorder, increases the risk of fetal overgrowth and later metabolic morbidity in the offspring. The placenta likely mediates these sequelae, but the exact mechanisms remain elusive. Mitochondrial dynamics refers to the joining and division of these organelles, in attempts to maintain cellular homeostasis in stress conditions or alterations in oxygen and fuel availability. These remodeling processes are critical to optimize mitochondrial function, and their disturbances characterize diabetes and obesity. Methods and results Herein we show that placental mitochondrial dynamics are tilted toward fusion in GDM, as evidenced by transmission electron microscopy and changes in the expression of key mechanochemical enzymes such as OPA1 and active phosphorylated DRP1. In vitro experiments using choriocarcinoma JEG-3 cells demonstrated that increased exposure to insulin, which typifies GDM, promotes mitochondrial fusion. As placental ceramide induces mitochondrial fission in pre-eclampsia, we also examined ceramide content in GDM and control placentae and observed a reduction in placental ceramide enrichment in GDM, likely due to an insulin-dependent increase in ceramide-degrading ASAH1 expression. Conclusions Placental mitochondrial fusion is enhanced in GDM, possibly as a compensatory response to maternal and fetal metabolic derangements. Alterations in placental insulin exposure and sphingolipid metabolism are among potential contributing factors. Overall, our results suggest that GDM has profound impacts on placental mitochondrial dynamics and metabolism, with plausible implications for the short-term and long-term health of the offspring.
Prevalence and trends of hepatitis B virus, Hepatitis C virus, Human immunodeficiency virus 1, 2 and syphilis infections among blood donors in a regional transfusion center in Punjab, India: A 3 years study

Background: Accurate estimates of the risk of transfusion-transmitted infectious diseases are essential for monitoring the safety of blood supply and evaluating the potential effects of new screening tests. Objective: The aim was to determine changes over time in blood donor population infection rates of hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV-1, 2) and syphilis. Materials and Methods: Changes in rates of HBV, HCV, HIV-1, 2, and syphilis infections were evaluated by comparing yearly prevalence rates for blood donors over 3 years, that is, between January 1, 2012 and December 31, 2014. Serological tests were done according to the standard operating procedures and manufacturer's instructions and included the following: tests for hepatitis B surface antigen; antibodies to HCV and HIV-1, 2 and rapid plasma reagin test for syphilis. Results: Nearly 2.54 of the total screened blood donors were reactive for one of the four transfusion transmitted infections (TTIs) with higher prevalence in replacement (3%) than voluntary donors (2.3%) and in male (2.54%) than female (2.3%) donors. TTI tend to be more (54.7%) in younger population of 18–30 years. HCV infection is the most common of all TTI (50%). Conclusion: The rising prevalence rates of HIV; HBV; HCV and syphilis among different groups suggests that blood transfusion is still very unsafe in this community and emphasis should be laid on donor education and donor self-exclusion, implementation of strict donor screening criteria, pre-donation counseling, and more sensitive screening methods. Furthermore, donors with a history of sexually transmitted infections should be totally excluded from all donations.
The Evolving Role of Gut Microbiota in the Management of Irritable Bowel Syndrome: An Overview of the Current Knowledge

The intestinal microbiota is one of the most rapidly evolving areas in biology and medicine. Extensive research in the last decade has escalated our understanding of the role of the microbiota in the pathogenesis of several intestinal and extra-intestinal disorders. Marked by high prevalence, substantial morbidity, and enormous costs, irritable bowel syndrome (IBS) is an important chronic gastrointestinal disorder that is widely encountered by gastroenterologists. Despite advances in our understanding of its pathophysiology, curative interventions have yet to be discovered, and therapeutic approaches remain symptom-driven. Recently, accumulating evidence has enlightened the possible impact of an imbalanced gut microbiome in the pathogenesis of IBS. In fact, several studies have documented altered microbiota in patients, while others have shown that IBS severity was associated with a distinct microbiota signature. These findings may pave the way for the use of microbiota manipulation strategies as an attractive option for IBS management, and may have an essential role in efforts to reduce the societal and economic effects of this ever-growing disorder. In this review, we have outlined the results of the latest research on the association between microbiota and IBS and their implications for the clinical management of affected patients.
New Data, Old Story: Molecular Data Illuminate the Tribal Relationships among Rove Beetles of the Subfamily Staphylininae (Coleoptera: Staphylinidae)

The megadiverse subfamily Staphylininae traditionally belonged to the best-defined rove beetle taxa, but the advent of molecular phylogenetics in the last decade has brought turbulent changes to the group’s classification. Here, we reevaluate the internal relationships among the tribes of Staphylininae by implementing tree inference methods that suppress common sources of systematic error. In congruence with morphological data, and in contrast to some previous phylogenetic studies, we unambiguously recover Staphylininae and Paederinae as monophyletic in the traditional sense. We show that the recently proposed subfamily Platyprosopinae (Arrowinus and Platyprosopus) is a phylogenetic artefact and reinstate Arrowinus as a member of Arrowinini stat. res. and Platyprosopus as a member of Platyprosopini stat. res. We show that several recent changes to the internal classification of the subfamily are phylogenetically unjustified and systematically unnecessary. We, therefore, reestablish Platyprosopini, Staphylinini, and Xantholinini as tribes within Staphylininae (all stat. res.) and recognize Coomaniini as a tribe (stat. nov.) rather than subfamily. Consequently, the traditional ranks of the subtribes Acylophorina, Afroquediina, Amblyopinina, Antimerina, †Baltognathina, Cyrtoquediina, Erichsoniina, Hyptiomina, Indoquediina, Quediina, and Tanygnathinina are restored (all stat. res.). We review the current classification of Staphylininae and discuss sources of incongruence in multigene phylogenies.
Rif1 Functions in a Tissue-Specific Manner To Control Replication Timing Through Its PP1-Binding Motif

Replication initiation in eukaryotic cells occurs asynchronously throughout S phase, yielding early- and late-replicating regions of the genome, a process known as replication timing (RT). RT changes during development to ensure accurate genome duplication and maintain genome stability. To understand the relative contributions that cell lineage, cell cycle, and replication initiation regulators have on RT, we utilized the powerful developmental systems available in Drosophila melanogaster. We generated and compared RT profiles from mitotic cells of different tissues and from mitotic and endocycling cells of the same tissue. Our results demonstrate that cell lineage has the largest effect on RT, whereas switching from a mitotic to an endoreplicative cell cycle has little to no effect on RT. Additionally, we demonstrate that the RT differences we observed in all cases are largely independent of transcriptional differences. We also employed a genetic approach in these same cell types to understand the relative contribution the eukaryotic RT control factor, Rif1, has on RT control. Our results demonstrate that Rif1 can function in a tissue-specific manner to control RT. Importantly, the Protein Phosphatase 1 (PP1) binding motif of Rif1 is essential for Rif1 to regulate RT. Together, our data support a model in which the RT program is primarily driven by cell lineage and is further refined by Rif1/PP1 to ultimately generate tissue-specific RT programs.
Activation Effects of Carnosine- and Histidine-Containing Dipeptides on Human Carbonic Anhydrases: A Comprehensive Study

l-Carnosine (β-Ala-l-His) and several other histidine-containing peptides, including two N-methylated forms on the imidazole ring (l-anserine and l-balenine), two derivatives modified on the carboxyl function (carcinine and l-carnosinamide), two analogues differing in the length of the N-terminal residue (l-homocarnosine and Gly-l-His) and the N-acetyl derivatives, were investigated as activators of four isoforms of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1). The four human isoforms hCA I, II, VA and IX were activated in the low to high micromolar range, with a rather complex structure activity relationship. A performed computational study allowed us to rationalize these results and to propose a binding mode of these activators within the enzyme active site. Similarly to other CA activators, the here studied peptides could find relevant pharmacological applications such as in the management of CA deficiencies, for therapy memory and enhancing cognition or for artificial tissues engineering.
Back to the Breast: An Historical Overview of the Perceived Connections Between Sudden Infant Death Syndrome and Breastfeeding

In the late 19th century, physicians in the United States and Europe grew concerned about an increasingly visible subset of infant mortality: sudden infant death. Over the next 100 years, physicians worked variably to combat the problem, modifying and refining their conceptions of sudden infant mortality many times over the process. Physicians’ overlapping revisions of sudden infant mortality ultimately helped to produce the categorization of Sudden Infant Death Syndrome (SIDS), and their ensuing, fluctuating efforts to resolve this problem shed light on social and medical perceptions of the roles that biology, the environment, and infant care practices played in sudden infant death. SIDS’s official medical classification was a watershed; not only did the formal medical label establish its “authenticity” as a medical phenomenon, but the label also asserted the inexplicability of (at least some) sudden infant death episodes while simultaneously conveying that affected parents were deserving victims of a tragic loss. In the modern history of sudden infant death in the United States, breastfeeding, in particular, was understood variably as a possible cause for unnecessary infant mortality in the decades surrounding 1900; inconsequential to the occurrence of SIDS in the mid 1900s; and finally as an important and healthful way to reduce the risk for SIDS beginning in the late 1900s.
Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells

Resident/endogenous mesenchymal stromal cells function to promote the normal development, growth, and repair of tissues. Following premature birth, the effects of routine neonatal care (e.g. oxygen support and mechanical ventilation) on the biological properties of lung endogenous mesenchymal stromal cells is (L-MSCs) is poorly understood. New Zealand white preterm rabbits were randomized into the following groups: (i) sacrificed at birth (Fetal), (ii) spontaneously breathing with 50% O2 for 4 hours (SB), or (iii) mechanical ventilation with 50% O2 for 4h (MV). At time of necropsy, L-MSCs were isolated, characterized, and compared. L-MSCs isolated from the MV group had decreased differentiation capacity, ability to form stem cell colonies, and expressed less vascular endothelial growth factor mRNA. Compared to Fetal L-MSCs, 98 and 458 genes were differentially expressed in the L-MSCs derived from the SB and MV groups, respectively. Gene ontology analysis revealed these genes were involved in key regulatory processes including cell cycle, cell division, and angiogenesis. Furthermore, the L-MSCs from the SB and MV groups had smaller mitochondria, nuclear changes, and distended endoplasmic reticula. Short-term hyperoxia/mechanical ventilation after birth alters the biological properties of L-MSCs and stimulates genomic changes that may impact their reparative potential.
The Assessment of Psychosocial Work Conditions and Their Relationship to Well-Being: A Multi-Study Report

The aim of this multi-study report is to present a questionnaire that enables researchers and practitioners to assess and evaluate psychosocial risks related to well-being. In Study 1, we conducted a cross-sectional online-survey in 15 German companies from 2016 to 2017 to verify factor- and criterion-related validity. Data consisted of 1151 employee self-ratings. Exploratory and confirmatory factor analyses resulted in an eight-factor structure (CFI = 0.902, RMSEA = 0.058, and SRMR = 0.070). All scales held to excellent internal consistency values (α = 0.65–0.90) and were related significantly to well-being (r = 0.17–0.35, p < 0.001). A second, longitudinal study in 2018 showed satisfying convergent and discriminant validity (N = 293) to scales from KFZA and COPSOQ. Test-retest reliability (N = 73; α = 0.65–0.88, p < 0.05) was also good. The instrument provides incremental validity above existing instruments since it explains additional variance in well-being.
Facilitating Recovery of Daily Functioning in People With a Severe Mental Illness Who Need Longer-Term Intensive Psychiatric Services: Results From a Cluster Randomized Controlled Trial on Cognitive Adaptation Training Delivered by Nurses

Abstract Background Feasible and effective interventions to improve daily functioning in people with a severe mental illness (SMI), such as schizophrenia, in need of longer-term rehabilitation are scarce. Aims We assessed the effectiveness of Cognitive Adaptation Training (CAT), a compensatory intervention to improve daily functioning, modified into a nursing intervention. Method In this cluster randomized controlled trial, 12 nursing teams were randomized to CAT in addition to treatment as usual (CAT; n = 42) or TAU (n = 47). Daily functioning (primary outcome) was assessed every 3 months for 1 year. Additional follow-up assessments were performed for the CAT group in the second year. Secondary outcomes were assessed every 6 months. Data were analyzed using multilevel modeling. Results CAT participants improved significantly on daily functioning, executive functioning, and visual attention after 12 months compared to TAU. Improvements were maintained after 24 months. Improved executive functioning was related to improved daily functioning. Other secondary outcomes (quality of life, empowerment, negative symptoms) showed no significant effects. Conclusions As a nursing intervention, CAT leads to maintained improvements in daily functioning, and may improve executive functioning and visual attention in people with SMI in need of longer-term intensive psychiatric care. Given the paucity of evidence-based interventions in this population, CAT can become a valuable addition to recovery-oriented care.
Teamwork in the operating theatre : scientific principles of assessment and clinical applications

2 ACKNOWLEDGEMENTS . 4 DECLARATION. 5 PEER REVIEWED PUBLICATIONS AND PRESENTATIONS . 6 LIST OF TABLES . 13 LIST OF FIGURES . 16 THESIS AIMS . 18 CONTENTS. 19 1 PATIENT SAFETY & ADVERSE EVENTS IN THE OPERATING THEATRE. 27 1.1 Chapter Overview . 27 1.1 Patient Safety. 27 1.2 Improving Patient Safety: Conceptualisations of Adverse Events. 28 1.3 Patient Safety in the Operating Theatre . 34 1.3. 1 Magnitude, Nature and Preventability of Surgical Adverse Events . 34 1.4 Application of the Systems Approach to Surgical Outcomes and Adverse Events . 35 2 NON-TECHNICAL SKILLS, TEAMWORK AND SAFETY IN THE OPERATING THEATRE . 38 2.1 Chapter Overview . 38 2.2 Non-Technical Skills . 38 2.3 Non-Technical Skills in High-Risk Industries . 39 2.4 Teams and Teamwork . 42 2.4. 1 Team Leadership . 43 2.4. 2 Backup Behaviour . 44 2.4. 3 Mutual Performance Monitoring. 44 20 2.4. 4 Team Orientation . 45 2.4. 5 Adaptability . 45 2.4. 6 Coordinating Mechanisms . 45 2.5 Teamwork in the Operating Theatre . 46 2.6 Measuring Teamwork in the Operating Theatre . 48 2.6.
Inflamed target tissue provides a specific niche for highly expanded T-cell clones in early human autoimmune disease

Objective To profile quantitatively the T-cell repertoire in multiple joints and peripheral blood of patients with recent onset (early) or established rheumatoid arthritis (RA) using a novel next-generation sequencing protocol to identify potential autoreactive clones. Methods Synovium of patients with recent onset (early) RA (<6 months) (n=6) or established RA (>18 months) (n=6) was screened for T-cell clones by sequencing over 10 000 T-cell receptors (TCR) per sample. T cells from paired blood samples were analysed for comparison. From two patients synovial T cells were obtained from multiple inflamed joints. The degree of expansion of each individual clone was based on its unique CDR3 sequence frequency within a sample. Clones with a frequency of over 0.5% were considered to be highly expanded clones (HEC). Results In early RA synovium, the T-cell repertoire was dominated by 35 HEC (median, range 2–70) accounting for 56% of the TCR sequenced. The clonal dominance in the synovium was patient specific and significantly greater than in established RA (median of 11 HEC (range 5–24) in established RA synovium accounting for 9.8% of T cells; p<0.01). 34% (range 28–40%) of the most expanded T-cell clones were shared between different joints in the same patients, compared with only 4% (range 0–8%) between synovium and blood (p=0.01). Conclusions In RA, a systemic autoimmune disease, the inflamed synovium forms a niche for specific expanded T-cell clones, especially in early disease. This suggests that, at least in RA, autoreactive T cells should be addressed specifically in the inflamed tissue, preferably in the early phase of the disease.
Exploring the facebook experience: a new approach to usability

The focus of this paper is to explore social networking sites such as Facebook in order to understand their recent success and popularity. Recent developments within Web 2.0 have provided users with more freedom to create their own unique user experiences. The conflict between traditional usability methods and user experiences are addressed through carrying out a Heuristic Evaluation to assess how well Facebook complies with usability guidelines and by conducting a user study to unveil unique user experiences. The findings of this study calls for a more holistic method of evaluation that redefines usability to encompass the user experience in line with future technology.
Mitigating the Impact of HIV and AIDS on Education Supply, Demand and Quality

This chapter focuses on the relationship between HIV and education in countries with different levels of HIV prevalence. While HIV affects all education sectors, the chapter concentrates on issues in schools, with some attention to teacher training colleges.1 It surveys experience from sub-Saharan Africa and Asia and the Pacific, and the lessons learned from highand low-prevalence countries in those regions.
A Detailed Study of Seventy Cases of Non-Small Cell Carcinoma of Lung, Immunohistochemical Study and its Histo-Cytological Correlation

Aims and Objectives: The main aims of this study is, to subtype non-small cell lung carcinoma (NSCLC) on fine needle aspiration (FNA) and small biopsy specimen, using a minimal antibody panel, to evaluate the role of immune histochemical markers for accurate diagnosis of NSCLC & to correlate sensitivity of FNA and small biopsy specimen to sub classify NSCLC. Materials and Methods: It is a prospective study of total number of seventy cases of non-small cell lung carcinoma (NSCLC), diagnosed on immunohistochemistry (IHC) for the duration of January 2013 to February 2015. Patients’ clinical details, fine needle aspiration cytology reports, biopsy reports and IHC reports were used for the data. Results: The study results showed that, by using limited panel of IHC (TTF-1, P63, and Desmoglein 3), differentiation between adenocarcinoma and squamous cell carcinoma is possible. Sensitivity of sub typing adenocarcinoma and squamous cell carcinoma are 88% and 73% respectively. Sensitivity of sub typing adenocarcinoma and squamous cell carcinoma on biopsy are 85% and 70%. Conclusion: Sensitivity of sub typing adenocarcinoma and squamous cell carcinoma on biopsy are 82% and 100%, when only cytology and histopathological examination were taken into account. With the use of IHC, rare tumour like sarcomatoid carcinoma was also diagnosed. Hence, immunohistochemistry represents a complementary tool for the routine diagnosis of lung cancer and for the identification of the different histological types and prognostic factors. Sensitivity and accuracy of sub typing NSCLC are high with both guided aspiration and biopsy specimens.
An Educational Intervention to assess Knowledge Attitude Practice of pharmacovigilance among Health care professionals in an Indian tertiary care teaching hospital

Objective: To assess awareness of pharmacovigilance among the healthcare professionals and to evaluate the impact of an educational intervention for improving awareness of pharmacovigilance among physician, pharmacist and nurses in an Indian tertiary care teaching hospital. Material &Methods: A suitable self-administered Knowledge, attitude, practice (KAP) survey questionnaire was designed, validated using a method developed by Lynn M and survey was conducted among physician, nurses, pharmacist of kasturba hospital, where local hospital based ADR reporting system exist. An interactive educational intervention was designed for all participants of Pre-KAP questionnaire survey. The impact of effectiveness of educational intervention among health care professionals was evaluated by means of post-KAP questionnaire survey. The chi-square test and Oneway Analysis of variance was used for statistical calculations. Results: In our study a total of 255 health care professionals were responded and involved in the pre KAP and postKAP survey questionnaires. Health care professionals involved in the study were 85 Doctors, 85 Nurses and 85 pharmacists from each group. The overall response rates between pre intervention and post intervention was statistically significant between doctors, nurses and pharmacist (P value <0.001) shows that effectiveness of educational intervention for improving awareness of pharmacovigilance among physician, pharmacist and nurses. Conclusion: Imparting the knowledge and awareness of pharmacovigilance among the health care professionals by means of continues educational intervention would bring update knowledge of practice for drug safety into their every day clinical practice and also brings the adverse drug reactions (ADRs) reporting culture among them.