Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of Folic Acid in the Therapeutic Action of Nanostructured Porous Silica Functionalized with Organotin(IV) Compounds against Different Cancer Cell Lines

The synthesis, characterization and cytotoxic activity against different cancer cell lines of various mesoporous silica-based materials containing folate targeting moieties and a cytotoxic fragment based on a triphenyltin(IV) derivative have been studied. Two different mesoporous nanostructured silica systems have been used: firstly, micronic silica particles of the MSU-2 type and, secondly, mesoporous silica nanoparticles (MSNs) of about 80 nm. Both series of materials have been characterized by different methods, such as powder X-ray diffraction, X-ray fluorescence, absorption spectroscopy and microscopy. In addition, these systems have been tested against four different cancer cell lines, namely, OVCAR-3, DLD-1, A2780 and A431, in order to observe if the size of the silica-based systems and the quantity of incorporated folic acid influence their cytotoxic action. The results show that the materials are more active when the quantity of folic acid is higher, especially in those cells that overexpress folate receptors such as OVCAR-3 and DLD-1. In addition, the study of the potential modulation of the soluble folate receptor alpha (FOLR1) by treatment with the synthesized materials has been carried out using OVCAR-3, DLD-1, A2780 and A431 tumour cell lines. The results show that a relatively high concentration of folic acid functionalization of the nanostructured silica together with the incorporation of the cytotoxic tin fragment leads to an increase in the quantity of the soluble FOLR1 secreted by the tumour cells. In addition, the studies reported here show that this increase of the soluble FOLR1 occurs presumably by cutting the glycosyl-phosphatidylinositol anchor of membrane FR-α and by the release of intracellular FR-α. This study validates the potential use of a combination of mesoporous silica materials co-functionalized with folate targeting molecules and an organotin(IV) drug as a strategy for the therapeutic treatment of several cancer cells overexpressing folate receptors.
High-Intensity Interval Training Prescription for Combat-Sport Athletes.

CONTEXT Combat sports are composed of high-intensity actions (eg, attacks, defensive actions, and counterattacks in both grappling and striking situations depending on the specific sport) interspersed with low-intensity actions (eg, displacement without contact, stepping) or pauses (eg, referee stoppages), characterizing an intermittent activity. Therefore, high-intensity interval training (HIIT) is at the essence of combat-sport-specific training and is used as complementary training, as well. HIIT prescription can be improved by using intensity parameters derived from combat-sport-specific tests. Specifically, the assessment of physiological indexes (intensity associated with the maximal blood lactate steady state, maximal oxygen consumption, and maximal sprint) or of time-motion variables (high-intensity actions, low-intensity actions, and effort:pause ratio) is a key element for a better HIIT prescription because these parameters provide an individualization of the training loads imposed on these athletes. PURPOSE To present a proposal for HIIT prescription for combat-sport athletes, exemplifying with different HIIT protocols (HIIT short intervals, HIIT long intervals, repeated-sprint training, and sprint interval training) using combat-sport-specific actions and the parameters for the individualization of these protocols. CONCLUSIONS The use of combat-sport-specific tests is likely to improve HIIT prescription, allowing coaches and strength and conditioning professionals to elaborate HIIT short intervals, HIIT long intervals, repeated-sprint training, and sprint interval training protocols using combat-sport actions, providing more specificity and individualization for the training sessions.
Bisphosphonate-Based Molecules as Potential New Antiparasitic Drugs

Neglected tropical diseases such as Chagas disease and leishmaniasis affect millions of people around the world. Both diseases affect various parts of the globe and drugs traditionally used in therapy against these diseases have limitations, especially with regard to low efficacy and high toxicity. In this context, the class of bisphosphonate-based compounds has made significant advances regarding the chemical synthesis process as well as the pharmacological properties attributed to these compounds. Among this spectrum of pharmacological activity, bisphosphonate compounds with antiparasitic activity stand out, especially in the treatment of Chagas disease and leishmaniasis caused by Trypanosoma cruzi and Leishmania spp. , respectively. Some bisphosphonate compounds can inhibit the mevalonate pathway, an essential metabolic pathway, by interfering with the synthesis of ergosterol, a sterol responsible for the growth and viability of these parasites. Therefore, this review aims to present the information about the importance of these compounds as antiparasitic agents and as potential new drugs to treat Chagas disease and leishmaniasis.
Effects of tyrosine kinase inhibitor therapy on skin toxicity and skin-related quality of life in patients with lung cancer

Abstract Epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) therapy is the primary treatment option for patients with non-small cell lung cancer (NSCLC). However, one of the major adverse effects associated with this therapy is skin toxicity, which impacts the patient's quality of life. This study aimed to describe the severities and locations of skin toxicity, and to analyze their association with the quality of life in patients with advanced NSCLC who received EGFR-TKI therapy as first-line treatment. This cross-sectional and correlation study was conducted at a tertiary medical center in northern Taiwan between July 2015 and March 2016. Skin toxicity was assessed and graded using the National Cancer Institute Common Terminology Criteria for Adverse Events (version 4.03). The Skindex-16 scale was used to measure the skin disease-related quality of life. A total of 146 NSCLC patients who received EGFR-TKI therapy within the first 3 months of diagnosis were included in this study; 93.2% of these patients experienced skin toxicities. Approximately 70% of the patients developed xerosis and pruritus, while 50% had papulopustular eruptions and paronychia. The mean skin symptom impact score was 5.38 (standard deviation = 2.65). The skin-related quality of life varied widely among the participants but remained acceptable (mean score = 13.96, standard deviation = 16.55). Skin symptoms correlated significantly with poor quality of life (r = 0.50, P < .001). Younger patients and those treated with afatinib were the most affected, reporting the poorest quality of life. Patients who required EGFR-TKI dose reduction had experienced more severe skin symptoms than had patients who did not require it (7.35 vs 5.01, P < .001). Skin toxicity related to EGFR-TKI treatment impacts the quality of life in patients with NSCLC. During the treatment period, skin assessment and tailored management should be incorporated into the daily care plan.
Reponse des vignes champenoises aux temperatures negatives: effet d'un refroidissement controle sur les reserves glucidiques du complexe gemmaire avant et au cours du debourrement

Sugar concentrations were analyzed in grapevine (Vitis vinifera) buds at three different phenological stages (stage 1, dormant bud; stage 2, woolly bud; stage 5, green tip) and were submitted, under controlled conditions, to negative temperatures corresponding to the intracellular liquid crystallization points. In absence of any treatment, starch concentration diminished according to the phenological evolution, indicating its utilization during bud breaking. Starch and raffinose accumulated when controlled cooling was applied, except for stage 1 buds collected on March 6, 1993. Similarly, saccharose concentrations were reduced in bud complexes after temperature reductions. Fructose and glucose concentrations seemed to be less sensitive to thermal variations. The accumulation of starch observed at the end of a cold treatment could be partly explained by the inhibition of amylase activity under cold conditions. Keywords: bud, Vitis vinifera L. , phenological stage, thermal stress, sugars. [Journal translation]
Effect of Stress Management Counseling on Self-Efficacy and Continuity of Exclusive Breastfeeding.

Background: Self-efficacy is an important motivational factor that can be affected by physiological responses such as stress. Objective: The study aimed to determine the effect of stress management counseling on self-efficacy and continuity of exclusive breastfeeding in mothers. Materials and Methods: This randomized controlled trial was carried out on 46 pregnant women recruited at three Childbirth Preparation Centers of Zanjan (Iran) in 2018. The eligible women were allocated into two intervention and control groups according to the block design. Stress management counseling was carried out individually in four sessions, twice a week at 35 and 36 weeks of gestation. The control group only received routine cares. The self-efficacy and continuity of exclusive breastfeeding were measured monthly up to 4 months after childbirth. Results: Breastfeeding self-efficacy showed a statistically significant difference between the two study groups at 1 and 4 months after childbirth (p = 0.001). More women in the control group terminated exclusive breastfeeding compared to those in the intervention group (16 (72.7%) versus 8 (34.8%), p = 0.013). Conclusion: The results showed that integration of stress management counseling in breastfeeding education package can improve the self-efficacy and continuation of breastfeeding in mothers.
Spontaneous splenic rupture 1 week after laparoscopic appendectomy due to acute appendicitis

Spontaneous splenic rupture is a rare entity that requires high index of suspicion for diagnosis. Usually, it occurs due to underlying pathology that could be inflammatory, neoplastic or infectious. However, there are also cases of spontaneous splenic rupture in a normal-sized spleen without obvious pathologic process. In our case, the patient suffered a spontaneous splenic rupture 1 week after laparoscopic appendectomy due to acute appendicitis. Histopathologic examination revealed a normal-sized spleen without any obvious pathology. In our patient, we did not found any explanation for a spontaneous splenic rupture, besides her primary inflammatory condition.
Knowledge, Attitude and Practices towards the Tiger Mosquito Aedes Albopictus. A Questionnaire Based Survey in Lazio Region (Italy) before the 2017 Chikungunya Outbreak

The invasion of Aedes albopictus has played a major role in the resurgence of mosquito-borne diseases in Italy, generating the two largest chikungunya outbreaks in Europe (2007, 2017). Knowledge, attitude and practice (KAP) are important in order to prevent Aedes-borne disease transmission, yet so far they have not been assessed. To this scope we used multivariate logistic regression to investigate KAP of citizen-to-Aedes ecology and transmitted diseases. Data were collated by a structured questionnaire (18 questions) in 2016. Participants were selected in the Lazio region from members of native populations and two resident communities (RC) originating from the Indian subcontinent where Aedes-transmitted diseases are endemic. Results showed that compared to Italians, RC respondents had a higher knowledge and concern of Aedes-transmitted diseases (Odds Ratio = 2.61 (95%CI: 1.03–6.05); OR = 3.13 (2.15–4.65)) as well as their life cycles (OR = 2.49 (1.75–3.56); OR = 9.04 (6.22–13.66)). In contrast, they perceived a lower nuisance due to the presence of Ae. albopictus (OR = 0.2 (0.13–0.32); OR = 0.55 (0.38–0.78). These findings suggest that citizens in the Lazio region are not prepared to face a potential outbreak of arboviruses and further efforts should be made to increase knowledge, awareness and best practices.
Observations on the biology of the greentail prawn, Metapenaeus mastersii (Haswell) (Crustacea decapoda: Penaeidae)

The ecology, growth, and behaviour of Metapenaeus mastersii from the Brisbane River, Qld. , are described. Postlarval stages were found to favour a warm sheltered locality with abundant algal cover and salinity below 20%,. Salinity tolerance range of all stages was from almost fresh to sea-water, and activity was at a minimum when the average temperature fell to 16'C. Carapace length was found to be as reliable a measure of size as overall length or volume. Progression of the modes of length frequency distributions was unsatisfactory for finding growth rate, but showed a steady downstream movement as growth progressed. From the growth rate of captive prawns, age at sexual maturity was estimated as 12-15 months at 16 mm carapace length (males) and 20 mm (females) (76 and 95 mm overall). Males were less tolerant of low salinity than females, but did not migrate as far downstream. Fertilization occurred in the lower reaches of the Brisbane River, but spawning occurred outside. The length-volume relationship of prawns above 10 mm carapace length (48 mm overall) did not conform to the curve: volume = k × (1ength). This deviation was interpreted as due to change of growth characteristics during winter. A reversed respiratory current and other adaptations to burying in the mud, and a possible associated diurnal rhythm are described.
Coronavirus Disease (COVID-19): A Machine Learning Bibliometric Analysis.

BACKGROUND/AIM To evaluate the research trends in coronavirus disease (COVID-19). MATERIALS AND METHODS A bibliometric analysis was performed using a machine learning bibliometric methodology. Information regarding publication outputs, countries, institutions, journals, keywords, funding and citation counts was retrieved from Scopus database. RESULTS A total of 1883 eligible papers were returned. An exponential increase in the COVID-19 publications occurred in the last months. As expected, China produced the majority of articles, followed by the United States of America, the United Kingdom and Italy. There is greater collaboration between highly contributing authors and institutions. The "BMJ" published the highest number of papers (n=129) and "The Lancet" had the most citations (n=1439). The most ubiquitous topic was COVID-19 clinical features. CONCLUSION This bibliometric analysis presents the most influential references related to COVID-19 during this time and could be useful to improve understanding and management of COVID-19.
PII: S1044-0283(99)00013-7

This paper examines the risks and returns of Latin American stocks following American depository receipt (ADR) listings in U.S. equity markets and finds no systematic change in their volatility. This finding differs from previous results for ADR introduction on European and Asian stocks, although it is consistent with several prior findings on international stock listings. Importantly, it supports the predictions of Domowitz, Glen, and Madhavan’s 1998 model of international cross-listings. This model predicts that the effects of such listings will differ across stocks because the net effect is indicative of the specific trade-off for each individual stock between benefits of enhanced intermarket competition and costs stemming from the diversion of information-linked orders out of the domestic market.  2000 Elsevier Science Inc. All rights reserved. JEL classification: G15; G14
Investigation on the status of oral intake management measures during labor in China

Abstract The World Health Organization recommended that the oral intake of low-risk pregnant women during labor should not be restricted. Hospitals in different countries take different measures to manage the intake during labor, but it is not clear about the current situation of oral intake management measures in the hospital during labor in China. Thus, the objective of this study was to investigate the current situation of oral intake management measures during labor in China, so as not only provide references for developing appropriate midwifery technology training and formulating relevant policies, but also provide a basis for exploring and implementing better oral intake management measures in the future. A cross-sectional survey was conducted. From December 2017 to November 2018, the oral intake management measures of 1213 hospitals in 22 provinces, cities, and autonomous regions in China were investigated by a self-designed questionnaire. χ2 test was used for statistical analysis. Different hospitals in China have adopted different oral intake management measures. Among the 1213 hospitals, 939(77.4%) hospitals took measures to allow pregnant women to bring the easily digestible food, 813(67.0%) hospitals took measures to allow pregnant women to eat what she wanted to eat. Few hospitals provide pregnant women with oral nutrition solution or provide a suitable diet for pregnant women. Thirty-four (2.8%) hospitals still restrict pregnant women's fluid intake. Oral intake management measures that are more suitable for Chinese pregnant women should be explored to better ensure the women energy needs and they safely go through childbirth.
CASH vs STOCK PAYMENT IN MERGERS AND ACQUISITIONS (M&As): DETERMINANTS AND SHAREHOLDER VALUATION

The aim of this paper is to study the influence of the Merger and Acquisition (M&A) payment method decision on the acquiring shareholders’ M&A valuation, considering the relevance of the acquiring ownership structure and the legal and institutional environment, and the possibility that the payment method decision may be endogenous. The results show that the ownership structure and the legal and institutional environment influence both the choice of payment method and the acquiring shareholders’ valuation. High levels of ownership concentration in the acquiring firm or in countries with strong legal and institutional environments reduce the probability of cash paid M&As. Acquiring shareholders value cash paid M&As more in firms with high levels of ownership concentration because of higher managerial control. Also, it is higher in countries with a strong legal and institutional environment, where lower agency costs are expected, and cash payment is not associated with avoiding ownership dilution. JEL Classification: G30; G32; G34; F30.
Evaluation of Erythromycin as a Tool to Assess CYP3A Contribution of Low Clearance Compounds in a Long-Term Hepatocyte Culture

Long-term hepatocyte culture systems such as HepatoPac are well suited to evaluate the metabolic turnover of low clearance (CL) drugs because of their sustained metabolic capacity and longer-term viability. Erythromycin (ERY), a moderate, mechanism-based inhibitor of CYP3A, was evaluated as a tool in the HepatoPac model to assess contribution of CYP3A to the clearance of drug candidates. ERY inhibited CYP3A activity by 58% and 80% at 3 and 10 μM, respectively, for up to 72 hours. At 30 µM, ERY inhibited midazolam hydroxylation by >85% for the entire 144-hour duration of the incubation. Alprazolam CLint was inhibited 58% by 3 μM of ERY, 75% by 15 μM of ERY, 89% by 30 μM of ERY, and 94% by 60 μM of ERY. ERY (30 μM) did not markedly affect CLint of substrates for several other major cytochrome P450 isoforms evaluated and did not markedly inhibit uridine diphosphoglucuronosyl transferase (UGT) isoforms 1A1, 1A3, 1A4, 1A6, 1A9, 2B7, or 2B15 as assessed using recombinant UGTs. ERY only mildly increased CYP3A4 gene expression by 2.1-fold (14% of rifampicin induction) at 120 µM, indicating that at effective concentrations for inhibition of CYP3A activity (30–60 µM), arylhydrocarbon receptor, constitutive androstane receptor, and pregnane-X-receptor activation are not likely to markedly increase levels of other drug-metabolizing enzymes or transporters. ERY at concentrations up to 60 µM was not toxic for up to 6 days of incubation. Use of ERY to selectively inhibit CYP3A in high-functioning, long-term hepatocyte models such as HepatoPac can be a valuable strategy to evaluate the contribution of CYP3A metabolism to the overall clearance of slowly metabolized drug candidates. SIGNIFICANCE STATEMENT This work describes the use of erythromycin as a selective inhibitor of CYP3A to assess the contribution of CYP3A in the metabolism of compounds using long-term hepatocyte cultures.
Optimal control of plant root tip dynamics in soil

This paper aims to propose a novel approach to model the dynamics of objects that move within the soil, e.g. plants roots. One can assume that external forces are significant only at the tip of the roots, where the plant’s growth is actuated. We formulate an optimal control problem that minimises the energy spent by a growing root subject to physical constraints imposed by the surrounding soil at the tip. We study the motion strategy adopted by plant roots to facilitate penetration into the soil, which we hypothesize to be a circumnutation movement. By solving the proposed optimal control problem numerically, we validate the hypothesis that plant roots adopt a circumnutation motion pattern to reduce soil penetration resistance during growth. The proposed formalisation could be applied to replicate such a biological behaviour in robotic systems, to adopt the most efficient strategy for autonomous devices in soil exploration.
Prognostic value of postoperative serum carcinoembryonic antigen levels in colorectal cancer patients who smoke

Serum carcinoembryonic antigen (CEA) levels can help predict the prognosis of colorectal cancer patients. Accordingly, high preoperative CEA levels that is not restored after surgery are indicative of a worse outcome. On the other hand, smoking can increase serum CEA levels independently of the disease status. Thus, we aimed to evaluate the impact of smoking on the prognostic value of serum CEA levels. This retrospective cohort study included 273 patients who underwent curative resection for stage I–III colorectal adenocarcinoma at a single institution, between January 2010 and December 2017. Patients were grouped as follows: group A, normal preoperative and postoperative CEA levels (n = 152); group B, elevated preoperative CEA levels that returned to reference values after surgery (n = 69); and group C, elevated postoperative serum CEA levels (n = 52). Patients were also grouped according to their smoking history: group S (current smokers, n = 79) and group NS (never and former smokers, n = 194). Group A showed a higher 3-year disease-free survival (DFS) rate (84.9%) than groups B (75.4%) and C (62.0%) (p < 0.001). Postoperative serum CEA levels were significantly higher in the S group than in the NS group (2.6 vs. 3.1 ng/mL, p = 0.009), whereas preoperative levels were similar (3.8 vs. 4.1, p = 0.182). Further, smokers showed higher 3 year-DFS rates than nonsmokers in group C (83.3% vs. 43.9%, p = 0.029). This suggests that while elevated postoperative CEA levels are associated with lower DFS rates in never and former smokers, they are not associated with lower DFS rates in current smokers. We conclude that persistent smoking alters the prognostic value of postoperative serum CEA levels in colorectal cancer patients and that, consequently, alternative surveillance strategies need to be developed for colon cancer patients with smoking habits.