Miracle-dz/UniFAR-FTUs huggingface dataset:
A Retrospective and Prospective Cohort Study Comparing Pediatric Patients With Cleft Lip and Palate From the United States and Guatemala

Orofacial clefts (OFC) remain among the most prevalent congenital abnormalities worldwide. In the United States in 2010 to 2014, 16.2 of 10,000 live births are born with OFC compared with 23.6 of 10,000 in Alta Verapaz, Guatemala in 2012. Demographics and cleft severity scores were retrospectively gathered from 514 patients with isolated OFC at the Children’s Hospital of Philadelphia scheduled for surgery from 2012 to 2019 and from 115 patients seen during surgical mission trips to Guatemala City from 2017 to 2020. Risk factors were also gathered prospectively from Guatemalan families. The Guatemalan cohort had a significantly lower prevalence of cleft palate only compared with the US cohort, which may be a result of greater cleft severity in the population or poor screening and subsequent increased mortality of untreated cleft palate. Of those with lip involvement, Guatemalan patients were significantly more likely to have complete cleft lip, associated cleft palate, and right-sided and bilateral clefts, demonstrating an increased severity of Guatemalan cleft phenotype. Primary palate and lip repair for the Guatemalan cohort occurred at a significantly older age than that of the US cohort, placing Guatemalan patients at increased risk for long-term complications such as communication difficulties. Potential OFC risk factors identified in the Guatemalan cohort included maternal cooking-fire and agricultural chemical exposure, poor prenatal vitamin intake, poverty, and risk factors related to primarily corn-based diets. OFC patients who primarily rely on surgical missions for cleft care would likely benefit from more comprehensive screening and investigation into risk factors for more severe OFC phenotypes.
Tidal Disruption Events and stellar-mass black holes in OGLE and Gaia surveys

Abstract Tidal Disruption Events (TDE) allow to probe the super massive black holes (SMBH) in the cores of galaxies and could be a source of black hole mass growth. We present the search for candidates for TDEs conducted within OGLE and Gaia surveys. Our preliminary results indicate that TDEs can occur in cores of galaxies exhibiting different levels of activity, from quiescent, through weak-AGNs to highly active QSOs. We also present how Gaia can help study the mass distribution of Milky Way single black holes via microlensing.
Poor Outcome in Postpartum Breast Cancer Patients Is Associated with Distinct Molecular and Immunologic Features

Abstract Purpose: Patients with postpartum breast cancer diagnosed after cessation of breastfeeding (postweaning, PP-BCPW) have a particularly poor prognosis compared with patients diagnosed during lactation (PP-BCDL), or to pregnant (Pr-BC) and nulliparous (NP-BC) patients, regardless of standard prognostic characteristics. Animal studies point to a role of the involution process in stimulation of tumor growth in the mammary gland. However, in women, the molecular mechanisms that underlie this poor prognosis of patients with PP-BCPW remain vastly underexplored, due to of lack of adequate patient numbers and outcome data. Experimental Design: We explored whether distinct prognostic features, common to all breast cancer molecular subtypes, exist in postpartum tumor tissue. Using detailed breastfeeding data, we delineated the postweaning period in PP-BC as a surrogate for mammary gland involution and performed whole transcriptome sequencing, immunohistochemical, and (multiplex) immunofluorescent analyses on tumor tissue of patients with PP-BCPW, PP-BCDL, Pr-BC, and NP-BC. Results: We found that patients with PP-BCPW having a low expression level of an immunoglobulin gene signature, but high infiltration of plasma B cells, have an increased risk for metastasis and death. Although PP-BCPW tumor tissue was also characterized by an increase in CD8+ cytotoxic T cells and reduced distance among these cell types, these parameters were not associated with differential clinical outcomes among groups. Conclusions: These data point to the importance of plasma B cells in the postweaning mammary tumor microenvironment regarding the poor prognosis of PP-BCPW patients. Future prospective and in-depth research needs to further explore the role of B-cell immunobiology in this specific group of young patients with breast cancer.
Measuring chemical exchange saturation transfer exchange rates in the human brain using a particle swarm optimisation algorithm

The z‐spectrum contains many pools with different exchange rates and T2 values, which can make it difficult to interpret in vivo data and complicates the design of experiments aimed at providing sensitivity to one pool. This work aims to characterise the main pools observable with MRI at 7T in the human brain. To achieve this, we acquired z‐spectra at multiple saturation powers in the human brain at 7T. We used simulations to optimise the use of particle swarm optimisation (PSO) to fit these data, validating this approach using further simulations and creatine phantoms. We then used the PSO to fit data from grey and white matter for the pool size, exchange rate, and T2 of five proton pools (magnetisation transfer, amides, amines, nuclear Overhauser enhancement NOE−3.5ppm and NOE−1.7ppm in addition to water). We then devised an approach for using PSO to fit z‐spectra while limiting the computational burden, and we investigated the sensitivity of the fit to T2 and k for three overlapping pools. We used this to measure the exchange rate of creatine and to show that it varied with temperature, as expected. In the brain we measured a significantly larger pool size in white matter than in grey matter for the magnetisation transfer pool and the NOE−3.5ppm pool. For all other parameters we found no significant difference between grey and white matter. We showed that PSO can be used to fit z‐spectra acquired at a range of B1 to provide information about peak position, amplitude, exchange rate, and T2 in vivo in the human brain. These data could provide more sensitivity to change in some clinical conditions and will also provide key information for further experimental design.
Isoflurane Conditioning‐Induced Delayed Cerebral Ischemia Protection in Subarachnoid Hemorrhage—Role of Inducible Nitric Oxide Synthase

Background Recent evidence implicates inflammation as a key driver in delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage (SAH). Inducible nitric oxide synthase (iNOS) is one of the known major mediators of inflammation. We previously showed that an inhalational anesthetic, isoflurane, provides strong protection against delayed cerebral ischemia after SAH. Our current study aims to define the role of iNOS in isoflurane conditioning‐induced protection against delayed cerebral ischemia in a mouse model of SAH. Methods and Results The experiments used 10‐ to 14‐week‐old male wild‐type (C57BL/6) and iNOS global knockout mice. Anesthetic conditioning was initiated 1 hour after SAH with isoflurane 2% for 1 hour. Isoflurane‐induced changes in iNOS expression were measured. N‐(3‐(aminomethyl) benzyl) acetamidine, a highly selective iNOS inhibitor, was injected intraperitoneally immediately after SAH and then daily. Vasospasm, microvessel thrombosis, and neurological assessment was performed. Data were analyzed by 1‐way ANOVA and 2‐way repeated measures ANOVA followed by Student Newman Keuls comparison test. Statistical significance was set at P<0.05. Isoflurane conditioning downregulated iNOS expression in naïve and SAH mice. N‐(3‐(aminomethyl) benzyl) acetamidine attenuated large artery vasospasm and microvessel thrombosis and improved neurological deficits in wild‐type animals. iNOS knockout mice were significantly resistant to vasospasm, microvessel thrombosis, and neurological deficits induced by SAH. Combining isoflurane with N‐(3‐(aminomethyl) benzyl) acetamidine did not offer extra protection, nor did treating iNOS knockout mice with isoflurane. Conclusions Isoflurane conditioning‐induced delayed cerebral ischemia protection appears to be mediated by downregulating iNOS. iNOS is a potential therapeutic target to improve outcomes after SAH.
METTL3‐mediated m6A modification of pri‐miR‐148a‐3p affects prostate cancer progression by regulating TXNIP

Prostate cancer (PCa) severely affects men's health worldwide. The mechanism of methyltransferase‐like 3 (METTL3) in affecting PCa development by regulating miR‐148a‐3p expression via N6‐methyladenosine (m6A) modification was investigated.
Encouraging adherence in adolescents with asthma using financial incentives: An RCT

Medication adherence in adolescents remains a significant management challenge and innovative strategies are needed to improve medication adherence. Financial incentives have been used to improve outcomes for health behaviors among adults, but have not been well‐studied among adolescents. The objective of this study was to test if a modest financial incentive improved medication adherence in adolescents with asthma compared with a control group.
Association of Depressive Symptom Trajectory With Physical Activity Collected by mHealth Devices in the Electronic Framingham Heart Study: Cohort Study

Background Few studies have examined the association between depressive symptom trajectories and physical activity collected by mobile health (mHealth) devices. Objective We aimed to investigate if antecedent depressive symptom trajectories predict subsequent physical activity among participants in the electronic Framingham Heart Study (eFHS). Methods We performed group-based multi-trajectory modeling to construct depressive symptom trajectory groups using both depressive symptoms (Center for Epidemiological Studies-Depression [CES-D] scores) and antidepressant medication use in eFHS participants who attended 3 Framingham Heart Study research exams over 14 years. At the third exam, eFHS participants were instructed to use a smartphone app for submitting physical activity index (PAI) surveys. In addition, they were provided with a study smartwatch to track their daily step counts. We performed linear mixed models to examine the association between depressive symptom trajectories and physical activity including app-based PAI and smartwatch-collected step counts over a 1-year follow-up adjusting for age, sex, wear hour, BMI, smoking status, and other health variables. Results We identified 3 depressive symptom trajectory groups from 722 eFHS participants (mean age 53, SD 8.5 years; n=432, 60% women). The low symptom group (n=570; mean follow-up 287, SD 109 days) consisted of participants with consistently low CES-D scores, and a small proportion reported antidepressant use. The moderate symptom group (n=71; mean follow-up 280, SD 118 days) included participants with intermediate CES-D scores, who showed the highest and increasing likelihood of reporting antidepressant use across 3 exams. The high symptom group (n=81; mean follow-up 252, SD 116 days) comprised participants with the highest CES-D scores, and the proportion of antidepressant use fell between the other 2 groups. Compared to the low symptom group, the high symptom group had decreased PAI (mean difference –1.09, 95% CI –2.16 to –0.01) and the moderate symptom group walked fewer daily steps (823 fewer, 95% CI –1421 to –226) during the 1-year follow-up. Conclusions Antecedent depressive symptoms or antidepressant medication use was associated with lower subsequent physical activity collected by mHealth devices in eFHS. Future investigation of interventions to improve mood including via mHealth technologies to help promote people’s daily physical activity is needed.
Numerical simulation and multiobjective optimization of a microchannel heat sink with arc-shaped grooves and ribs

ABSTRACT In order to obtain the optimal structure size of a microchannel heat sink (MCHS) with arc-shaped grooves and ribs according to the actual demand, multiple parameters that influence the performance of the microchannel are analyzed by combining the multi-objective evolutionary algorithm (MOEA) with computational fluid dynamics (CFD). The design variables include the relative groove height, relative rib height and relative rib width, and the two objective functions are to minimize the total thermal resistance and pumping power in constant volume flow rate. The influences of the design variables on the two objective functions are analyzed by CFD firstly. The results show that each design variable has a different impact on the two functions. The competitive relationship between the two objective functions is depicted in plots of the Pareto front obtained by MOEA. Pareto sensitivity analysis is carried out to find that the relative rib height has the most significant impact on the two objective functions.
Semantic externalism without thought experiments

1. Semantic externalism (henceforth: externalism) is the thesis that the contents of intentional states (such as beliefs) and speech acts (such as assertions) are not determined by the way the subjects of those states or acts are internally. Externalism is a widely accepted but not entirely uncontroversial thesis. Among widely accepted but not entirely uncontroversial theses in philosophy, externalism is notable for owing the assent it commands almost entirely to thought experiments—especially to Putnam’s (1975) ‘Twin Earth’ thought experiment. The latter is arguably—along with Gettier’s (1963) refutation of the justified true belief (JTB) analysis of knowledge— one of the two most influential thought experiments in the history of analytic philosophy. For this reason, like the view that knowledge is not JTB, one might treat externalism as a test case: is thought experimentation required for establishing the view, or are there some widely accepted general principles from which the view can be derived purely by deduction? Recently, Williamson (2013, 2015) has argued that the latter is the case for the view that knowledge is not JTB: the existence of counterexamples to the JTB analysis of knowledge can be shown using certain natural models for epistemic logic. This paper argues for a similar conclusion about externalism: it is a deductive consequence of a pair of widely accepted general principles whose relevance to the issue has so far gone unnoticed. To avoid any misunderstandings, I should say something about my motives for engaging in this intellectual exercise. A good deal of recent metaphilosophy exhibits hostility towards thought experimentation, as well as towards certain less clearly demarcated practices in the vicinity, variously described as ‘appealing to intuitions’, ‘using intuitions as evidence’, making ‘judgments about cases’, and the like. This paper has no such agenda. I believe that thought experiments can be used to acquire knowledge of important philosophical truths, and that various important philosophical
Valency patterns of manner of speaking verbs in Croatian

Abstract Manner of speaking verbs denote the transfer of a message through speech, emphasizing the volume, intensity, comprehensibility, psychophysical condition of the speaker, and/or the impression that the speaker leaves on the hearer. In this article, verbs are semantically divided into four subclasses: 1. Verbs with emphasis on volume, 2. verbs of incomprehensible speaking, 3. verbs of meaningless speaking and complaining, and 4. verbs with emphasis on emotional component. Their syntactic peculiarities have been extensively researched in English, while no special attention has been paid to these verbs in Croatian. It is stated that in Croatian they are monovalent verbs. However, these verbs can be bivalent, and even trivalent. The recipient can be expressed by a dative complement within all four semantic subclasses. With the verbs of loud speaking and verbs with negative emotions, it can be expressed by a prepositional complement na ‘at’ + accusative and za ‘after’ + instrumental. The theme can be expressed by a quotation and a clausal complement, a prepositional complement o ‘about’ + locative, an accusative complement, sometimes a prepositional complement protiv ‘against’ + genitive, za ‘for’ + accusative, and with fewer verbs with prepositional phrases za ‘for’ + instrumental or nad ‘over’ + instrumental. Interestingly, there are certain restrictions for the complements’ combination within the same clause, which are described in more detail in the article.
Propagation path of a flowering cherry (Cerasus × yedoensis) cultivar ‘Somei-Yoshino’ traced by somatic mutations

Flowering cherry cultivar ‘Somei-Yoshino’ (Cerasus × yedoensis) has been clonally propagated and spread all around the world including Japan. ‘Somei-Yoshino’ is thought to be an interspecific hybrid derived from C. spachiana and C. speciosa; however, its origin is unclear. Since somatic mutations are randomly induced in genomes and stably transmitted through generations, we aimed to identify somatic mutations in the genome of ‘Somei-Yoshino’ to trace its propagation path. A total of 46 ‘Somei-Yoshino’ clones were collected from all over Japan and subjected to whole-genome sequencing. The results revealed 684 single nucleotide variants, of which 71 were found in more than two clones. Clustering analysis of the 46 clones using these 71 variants revealed six groups, four of which contained 40 of the 46 clones. In addition, because each of the four clones closely planted in Ueno Park, Tokyo, Japan, clustered into the four different groups, we considered that these four clones could be the ancestors of the ‘Somei-Yoshino’ clones found in Japan. Furthermore, based on the comparison of mutant alleles with the genomes of Cerasus species, one of the four trees was concluded as the closest to the origin. Here, we propose that the origin of ‘Somei-Yoshino’ is a chimera derived from at least four somatic mutants.
Multidimensional super-resolution microscopy unveils nanoscale surface aggregates in the aging of FUS condensates

The intracellular liquid-liquid phase separation (LLPS) of biomolecules gives rise to condensates that act as membrane-less organelles with vital functions. FUS, an RNA-binding protein, natively forms condensates through LLPS and further provides a model system for the often disease-linked liquid-to-solid transition of biomolecular condensates during aging. However, the mechanism of such maturation processes, as well as the structural and physical properties of the system, remain unclear, partly attributable to difficulties in resolving the internal structures of the micrometer-sized condensates with diffraction-limited optical microscopy. Harnessing a set of multidimensional super-resolution microscopy tools that uniquely map out local physicochemical parameters through single-molecule spectroscopy, here we uncover nanoscale heterogeneities in the aging process of FUS condensates. Through spectrally resolved single-molecule localization microscopy (SR-SMLM) with a solvatochromic dye, we unveil distinct hydrophobic nanodomains at the condensate surface. Through SMLM with a fluorogenic amyloid probe, we identify these nanodomains as amyloid aggregates. Through single-molecule displacement/diffusivity mapping (SMdM), we show that such nanoaggregates drastically impede local diffusion. Notably, upon aging or mechanical shears, these nanoaggregates progressively expand on the condensate surface, thus leading to a growing low-diffusivity shell while leaving the condensate interior diffusion-permitting. Together, beyond uncovering fascinating nanoscale structural arrangements and aging mechanisms in the single-component FUS condensates, the demonstrated synergy of multidimensional super-resolution approaches in this study opens new paths for understanding LLPS systems.
Upscaled Synthesis of n‐ and p‐Type Thermoelectric Skutterudite Single Legs by Gas Atomization and Current‐Assisted Sintering

CoSb3‐based Skutterudites are among the best materials for thermoelectric generator (TEG) applications in the intermediate temperature range up to 500 °C. Synthesis of these materials is usually performed on a laboratory scale in materials research. In order to be suitable for an industrial low cost production of TEG technologies capable of delivering large amounts of thermoelectric (TE) materials are needed. A process mastering this challenge is gas atomization, which has been adapted to the requirements of TE materials, in particular CoSb3‐based Skutterudites . It is found that despite rapid solidification taking place in the atomization process the produced powder material contains only traces of the target Skutterudite phase. Microstructure investigation shows a very fine dispersion on the micrometer scale of CoSb, CoSb2, and Sb phases in the atomized particles, making diffusion paths for the formation of the Skutterudite phase short. This allows the use of short‐term heat treatment to achieve almost single phase material of high functional homogeneity. Different thermal post‐treatments are evaluated leading to a content of >98% of the Skutterudite phase in large ingots. Doping and filling by varying the starting composition is applied to tune the materials to n‐ and p‐type conduction, respectively, and led to an increase of their thermoelectric figure of merit ZT up to values of 0.9 and 0.72 for n‐ and p‐type material, respectively.
Effects of ocean acidification and high temperatures on the bryozoan<i>Myriapora truncata</i>at natural CO<sub>2</sub>vents

There are serious concerns that ocean acidiﬁcation will combine with the effects of global warming to cause major shifts in marine ecosystems, but there is a lack of ﬁeld data on the combined ecological effects of these changes due to the difﬁculty of creating large-scale, long-term exposures to elevated CO 2 and temperature. Here we report the ﬁrst coastal transplant experiment designed to investigate the effects of naturally acidiﬁed seawater on the rates of net calciﬁcation and dissolution of the branched calcitic bryozoan Myriapora truncata (Pallas, 1766). Colonies were transplanted to normal (pH 8.1), high (mean pH 7.66, minimum value 7.33) and extremely high CO 2 conditions (mean pH 7.43, minimum value 6.83) at gas vents off Ischia Island (Tyrrhenian Sea, Italy). The net calciﬁcation rates of live colonies and
arteriography for evaluation of cervical carotid Assessment of MR angiography versus

PURPOSE: To assess the accuracy of MR angiography alone in screening for vascular stenosis of the common carotid bifurcation. METHODS: Two hundred two common carotid bifurcations in 101 patients were evaluated with MR angiography and selective contrast arteriography. A two dimensional time-of-flight pulse sequence was used to obtain sequential transverse images through the common carotid bifurcations. These images were reprojected with a maximum intensity pixel ray-tracing algorithm. Both examinations were blindly graded as either normal or mildly stenotic (0%-29% ), moderately stenotic (30%-69% ), severely stenotic (70%-99% ), or occluded. RESULTS: Of the 202 common carotid bifurcations, 119 were classified as normal-mild stenosis by contrast arteriography. In this category, MR angiography correctly identified 114 of these as normal to mild stenosis. Among 21 common carotid bifurcations graded as moderate stenosis by arteriography, 15 were correctly graded as moderate by MR angiography. Among 45 common carotid bifurcations graded as severe stenosis by arteriography, 41 of these were correctly graded as severe by MR angiography. There were 17 complete occlusions which were all correctly graded by MR angiog raphy. CONCLUSION: MR angiography with its high rate of agreement with contrast arteriography can be regarded as an accurate screening method of the common carotid bifurcation.