Miracle-dz/UniFAR-FTUs huggingface dataset:
Arid4b is critical for mouse embryonic stem cell differentiation towards mesoderm and endoderm, linking epigenetics to pluripotency exit.

Distinct cell types emerge from embryonic stem cells through a precise and coordinated execution of gene expression programs during lineage commitment. This is established by the action of lineage specific transcription factors along with chromatin complexes. Numerous studies have focused on epigenetic factors that affect ESC self-renewal and pluripotency. However, the contribution of chromatin to lineage decisions at the exit from pluripotency has not been as extensively studied. Using a pooled epigenetic shRNA screen strategy, we identified chromatin related factors critical for differentiation towards mesodermal and endodermal lineages. Here we reveal a critical role for the chromatin protein, Arid4b. Arid4b-deficient mESCs are similar to wild-type mESCs in the expression of pluripotency factors and their self-renewal. However, Arid4b loss results in defects in upregulation of the meso/endodermal gene expression program. It was previously shown that Arid4b resides in a complex with Sin3a and Hdacs 1 and 2. We identified a physical and functional interaction of Arid4b with Hdac1 rather than Hdac2, suggesting functionally distinct Sin3a sub-complexes might regulate cell fate decisions Finally, we observed that Arid4b deficiency leads to increased H3K27me3 and reduced H3K27Ac level in key developmental gene loci, while a subset of genomic regions gain H3K27Ac marks. Our results demonstrate that epigenetic control through Arid4b plays a key role in the execution of lineage-specific gene expression programs at pluripotency exit.
Treatment Is More Than Prevention: Perceived Personal and Social Benefits of Undetectable = Untransmittable Messaging Among Sexual Minority Men Living with HIV.

Research suggests that the science of undetectable viral load (VL) status and HIV transmission-conveyed with the slogan "Undetectable = Untransmittable" or "U = U"-has gaps in acceptance despite robust scientific evidence. Nonetheless, growing acceptance of U = U creates conditions for a shift in the sociopolitical and personal implications of viral suppression. We conducted an online survey over a 23-month period in 2018 and 2019 among 30,361 adolescent and adult (aged 13-99) sexual minority men living with HIV (SMM-LHIV) across the United States. We examined the impact of U = U on self-image, potential for changing societal HIV stigma, whether SMM-LHIV had ever spoken with a provider about viral suppression and HIV transmission, and primary sources of hearing about U = U. Approximately 80% of SMM-LHIV reported that U = U was beneficial for their self-image and societal HIV stigma, 58.6% reported it made them feel "much better" about their own HIV status, and 40.6% reporting it had the potential to make HIV stigma "much better. " The most consistent factors associated with these beliefs centered around care engagement, particularly self-reported viral suppression and excellent antiretroviral therapy adherence. Two-thirds reported ever talking to a provider about VL and HIV transmission, although the primary sources for having heard about U = U were HIV and lesbian, gay, bisexual, transgender, and queer (LGBTQ) news media and personal profiles on networking apps. These findings demonstrate the significant personal and social importance of U = U for SMM-LHIV that go above-and-beyond the well-documented health benefits of viral suppression, suggesting that providers should consider routinely initiating conversations with patients around the multifaceted benefits (personal health, sexual safety and intimacy, increased self-image, and reduced social stigma) of viral suppression.
NEW SPECIES OF CA VERNICOLOUS CRABS OF THE GENUS SESARMOIDES FROM THE WESTERN PACIFIC, WITH A KEY TO THE GENUS (CRUSTACEA:DECAPODA:BRACHYURA:SESARMIDAE)

j ABSTRACT. -Five new species of cavemicolous sesarmid crabs of the genus Sesartnoides are described ' from Indonesia, Loyalty Islands, Philippines and Guam, viz., S. balicus, S. loyalty, S. sulu, S. boholano and S. guamensis. Diagnoses and detailed figures are provided for all species. The taxonomy of S. emdi Ng & Whitten, 1995, is also clarified, and a key to the 14 recognised species of Sesartnoides is provided.
Vegetation change in the grasslands and grassy woodlands of east-central Cape York Peninsula, Australia

The vegetation of 64 grassland and grassy woodland sites in east-central Cape York Peninsula, surveyed by CSIRO in 1966, was re-surveyed in 1995. While the original vegetation communities had persisted at most sites, a change in species dominance was recorded at 14% of sites. Melaleuca viridiflora (ti-tree) had invaded eight sites, and increased in abundance in at least 16 of the 35 sites in which it had occurred in 1966. This had led to four out of 13 grassland sites, and three out of four mixed evergreen sites being re-classified as ti-tree woodlands. Analysis of aerial photographs covering 415 km2 showed a 10% net loss of grasslands between 1969 and 1988. These changes are attributed to a reduction in the use of fire as a management tool since European settlement. Changes were also found in perennial grass composition consistent with grazing or over-grazing, particularly in the eucalypt/ironwood communities. A shift from Themeda triandra to Heteropogon contortus parallels earlier, grazing-related changes in southern Queensland. Evidence of a decline in Heteropogon triticeus and isolated losses of Sorghum plumosum are more suggestive of localized, unsustainable overgrazing. An increase in Sorghum plumosum in wet habitats appears to be associated with recent lack of burning. These changes suggest that further intensification of cattle grazing in the area may not be possible without significantly reducing both conservation values and pasture condition.
Health care utilization among Middle Eastern, Hispanic/Latino, and Asian immigrants in the United States: an application of Andersen’s behavioral model

ABSTRACT Objectives To examine whether Andersen’s model explains health care utilization among Middle Eastern immigrants and to examine gender and ethnic differences in health care utilization of Middle Eastern, Hispanic/Latino, and Asian immigrants in the United States. Method Using data from the 2000–2017 National Health Interview Surveys (NHIS), this study compares patterns of health care utilization among Middle Eastern (ME) immigrants to those among Hispanic/Latino and Asian immigrants in the U.S. Specifically, we use Andersen's Behavioral Model of Health Care Utilization to model the likelihood of seeing a doctor in the past 12 months for these three immigrant groups. Additionally, the current study emphasizes the predisposing factor of gender and its differences across and within these groups. Results Andersen's behavioral framework is partially suited to predict the likelihood of seeing a doctor in the past 12 months among ME immigrants. Immigrant women, regardless of their ethnicity, are significantly more likely than men to report seeing a doctor in the past 12 months. Moreover, the effects of the predisposing, need, and enabling characteristics for Hispanics and Asians are significantly different from those for ME immigrants. Additionally, although Asians are significantly less likely to utilize the health care system than ME immigrants, there are no significant differences comparing Hispanics/Latinos to ME immigrants. Furthermore, Andersen's behavioral framework explains ethnicity and gender differences particularly when comparing Asian men to ME men and Asian women to ME women. Conclusions This study highlights the importance of considering ethnicity and gender differences when examining health outcomes of immigrants.
Limit theory of isv-functions and its application for rough sets

In this paper, limit theory of set-valued functions defined on an interval (for short, isv-functions) is preliminarily established. Firstly, the concept of isv-functions is introduced. Secondly, limits of isv-functions are proposed and their properties are obtained. Thirdly, point-wise continuity of isv-functions and continuous isv-functions are discussed. Finally, an application of this theory for rough sets is given.
Inhibition of Chlorophyll Accumulation by Glyphosate

Glyphosate [N-(phosphonomethyl)glycine] significantly decreased the chlorophyll content of field-grown soybeans (Glycine max L. Merr. ‘Williams’) within 48 h after a 2.24-kg/ha treatment. In laboratory studies, 0.0001, 0.001, 0.01, 0.1, and 1 mM glyphosate reduced chlorophyll content of 7-day-old etiolated barley (Hordeum vulgare L. ‘Barsoy’) shoots 8, 12, 25, 49, and 77%, respectively, following 8 h dark incubation and 24 h illumination. Reduction of chlorophyll accumulation ranged from 6% with 0.001 mM glyphosate to 82% with 1.0 mM glyphosate. In studies with 8-day-old etiolated corn (Zea mays L. ‘Pioneer Brand 3535’) shoots, 0.1, 1.0, and 10.0 mM glyphosate decreased chlorophyll content of corn shoots 24, 42, and 50%, respectively, after 12 h of illumination. The rate of chlorophyll accumulation in corn shoots was significantly reduced 64% by 1.0 mM glyphosate over a 15-h illumination period. These rapid and substantial effects on chlorophyll accumulation suggest that interference with greening may be important in the mechanism of action of glyphosate.
Infection Control Practices and Outcomes of Endoscopy Units in the Lombardy Region of Italy

Supplemental Digital Content is available in the text. Goals: The present survey from the Italian Society of Digestive Endoscopy (SIED—Società Italiana di Endoscopia Digestiva) was aimed at reporting infection control practice and outcomes at Digestive Endoscopy Units in a high-incidence area. Background: Lombardy was the Italian region with the highest coronavirus disease-2019 (COVID-19) prevalence, at the end of March 2020 accounting for 20% of all worldwide deaths. Joint Gastro-Intestinal societies released recommendations for Endoscopy Units to reduce the risk of the contagion. However, there are few data from high-prevalence areas on adherence to these recommendations and on their efficacy. Methods: A survey was designed by the Lombardy section of SIED to analyze (a) changes in activity and organization, (b) adherence to recommendations, (c) rate of health care professionals’ (HCP) infection during the COVID-19 outbreak. Results: In total, 35/61 invited centers (57.4%) participated; most modified activities were according to recommendations and had filtering face piece 2/filtering face piece 3 and water-repellent gowns available, but few had negative-pressure rooms or provided telephonic follow-up; 15% of HCPs called in sick and 6% had confirmed COVID-19. There was a trend (P=0.07) toward different confirmed COVID-19 rates among endoscopists (7.9%), nurses (6.6%), intermediate-care technicians (3.4%), and administrative personnel (2.2%). There was no correlation between the rate of sick HCPs and COVID-19 incidence in the provinces and personal protective equipment availability and use, whereas an inverse correlation with hospital volume was found. Conclusions: Adherence to recommendations was rather good, though a minority were able to follow all recommendations. Confirmed COVID-19 seemed higher among endoscopists and nurses, suggesting that activities in the endoscopy rooms are at considerable viral spread risk.
Employer attitudes towards older workers in Australia

This research was concerned with the management of Australia’s labour supply, considering prospects for extending working lives against the backdrop of demographic change. Numerous factors have thrust the prolongation of Australians’ working lives to the forefront for policy-makers, researchers, non- government organisations and employers, including: the ageing of the Australian population; issues of social welfare, for example, warnings regarding the imminent retirement of so-called baby boomers expected to reduce available skilled workers, reduce labour force participation rates and raise dependency ratios; macroeconomic factors; and social justice factors, for example, discrimination against older workers in personnel decisions. Commentators argue the key policy challenges presented by population ageing are connected. The solution lies in increasing the engagement of older people in activity and dispelling the ageist views of an ageing population as inaccurate and counterproductive; this demographic shift should be viewed as an opportunity. Discrimination on the basis of age has been noted as an important barrier to labour force participation of older workers. Older workers’ labour market experiences have attracted research interest for decades. One facet has been employers’ attitudes. Eighteen studies have assessed employers’ attitudes towards older workers since initial work in the mid 1970s. The results of this research have been far from consistent in the demonstration of differences in employer attitudes across demographic and organisational characteristics and how these attitudes are associated with organisational practices. In the present study, 595 employers in medium and large organisations were surveyed on a range of workforce management issues. This study simultaneously assessed employer attitudes towards workers in age groups spanning a working life. Attitudes towards older workers were compared across organisational and individual characteristics and their association with organisational practices were assessed. Employers’ responses indicated positive and negative attitudes toward older workers that were interpreted overall as suggesting specific approaches to remove attitudinal barriers hindering the prolongation of working lives.
Quetiapine withdrawal: A systematic review

Objective: Withdrawal from psychoactive medication such as quetiapine is a well-documented phenomenon. Despite the extensive use of quetiapine, there have been few studies into the presence of discontinuation symptoms. We therefore performed a systematic review of published literature for evidence of quetiapine withdrawal or symptoms associated with discontinuation. Methods: We searched PubMed, Embase, CINAHL, Medline, Web of Science, PsycINFO for articles containing the terms ‘Quetiapine’ AND ‘withdraw$’ OR ‘discontinue$’. We included all study types that reported on somatic withdrawal symptoms and had no language restrictions. We excluded studies where there was withdrawal from multiple medications or any other psychoactive substance, or where the only symptoms were psychological such as rebound psychosis or craving. Results: We included 13 papers, all of which were individual case reports. The quality of the individual case reports was sub-optimal, as assessed by the CARE Case Report Guidelines. There was an association between rapid cessation of quetiapine and onset of somatic symptoms such as nausea, vomiting, agitation, restlessness, diaphoresis, irritability, anxiety, dysphoria, sleep disturbance, insomnia, tachycardia, hypertension and dizziness. Three studies also reported the onset of a withdrawal dyskinesia characterised by abnormal choreiform movements as well as confusion and speech disturbance in some cases. However, these findings were limited by the number and quality of case reports identified. Conclusion: Discontinuation symptoms are an uncommon side effect of quetiapine cessation, which may have clinical implications. Clinicians should therefore be alert to the possibility of quetiapine withdrawal in individuals who present with somatic symptoms or choreiform movements. However, large prospective studies are required to clarify this association.
Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors

Background Tertiary lymphoid structures (TLS) are associated with favorable survival and play a critical role in most solid tumors. However, investigations of TLS are lacking in patients with grade 1 or grade 2 (G1/G2) non-functional pancreatic neuroendocrine tumors (NF-PanNETs). This study aimed to investigate the presence, cellular composition, association with tumor-infiltrating immune cells, and prognostic value of TLS in G1/G2 NF-PanNETs. Methods Tumor tissues from a 182-patient Fudan cohort and a 125-patient external validation set were assessed by H&E staining, immunohistochemistry, and/or multispectral fluorescent immunohistochemistry. Results TLS were identified in more than one-third of patients with G1/G2 NF-PanNETs and were located peritumorally, either just outside the tumor tissue or in the stromal area. TLS were mainly composed of B-cell follicles with germinal centers and T-cell zones with dendritic cells. Kaplan-Meier analyses showed that the presence of TLS correlated with both longer recurrence-free survival (RFS, p<0.001) and overall survival (OS, p=0.001), but the number of TLS had no prognostic significance. Multivariate Cox-regression analyses demonstrated that the presence of TLS, WHO classification, and 8th edition American Joint Committee on Cancer (AJCC8th) tumor-node-metastasis (TNM) stage were independent prognostic factors for RFS (p=0.004, p=0.001, and p<0.001, respectively) and OS (p=0.009, p=0.008, and p=0.019, respectively). These results were confirmed using an external validation set. Finally, a nomogram incorporating the presence of TLS was constructed to predict the probability of 5-year RFS of resected G1/G2 NF-PanNETs, which improved on the current WHO classification and AJCC8th TNM stage. Conclusions The presence of TLS is an independent and favorable predictor of resected G1/G2 NF-PanNETs, which may play a role in cancer immunobiology.
How we use molecular minimal residual disease (MRD) testing in acute myeloid leukaemia (AML)

In recent years there have been major advances in the use of molecular diagnostic and monitoring techniques for patients with acute myeloid leukaemia (AML). Coupled with the simultaneous explosion of new therapeutic agents, this has sown the seeds for significant improvements to treatment algorithms. Here we show, using a selection of real‐life examples, how molecular monitoring can be used to refine clinical decision‐making and to personalise treatment in patients with AML with nucleophosmin (NPM1) mutations, core binding factor translocations and other fusion genes. For each case we review the established evidence base and provide practical recommendations where evidence is lacking or conflicting. Finally, we review important technical considerations that clinicians should be aware of in order to safely exploit these technologies as they undergo widespread implementation.
The effects of the mesomorphic ideal on men's body image, mood, self-esteem and muscle-building behaviour: Mechanisms of social comparison and body image investment

The purpose of this study was to determine whether individual differences is social comparison and body image investment moderate the relationship between media exposure to the mesomorphic ideal and male body image, self-esteem, mood and musclebuilding behaviour, as has been shown in women who are exposed to the thin media ideal (Dittmar & Howard, 2004). It was hypothesized that men with high social comparison tendencies as well as high body image investment would experience greater body dissatisfaction, lower self-esteem, negative mood, and greater muscle-building behaviour following exposure to mesomorphic images. Sixty-nine male undergraduate students participated. The results indicated that men who were less invested in their appearance and had a low social comparison tendency reported greater body dissatisfaction and lower appearance self-esteem after viewing mesomorphic images compared to viewing average images, whereas men high on these individual differences remained relatively unaffected. Furthermore, men who viewed mesomorphic images chose a heavier dumbbell to engage in bicep curls than did men who viewed average images.
Cloning,Sequencing and Evolution Analysis of IFN-γ,IL-2 and IL-4 Gene in Chinese White Rabbit

With reverse transcription polymerase chain reaction(RT-PCR),IFN-γ,IL-2 and IL-4 gene were gained from total RNA which was extracted from ConA-stimulated-cultured peripheral blood lymphocytes of Chinese white rabbit.The sequence analysis revealed that:①Compared with one published sequence(sequence number:AB010386),the homology of nucleotide sequence and amino acid sequence of IFN-γgene(sequence number:DQ852341) were both 100%;compared with other mammals in Genbank,including dog,cat,human,pig,cow,horse,goat,giant panda,rat,and so on,the homology of nucleotide sequence was between 63.0%(Rat) and 77.6%(Human),the homology of amino acid sequence was between 41.7%(Rat) and 65.7%(Human);②Compared with one published sequence(sequence number:AF068057),the homology of nucleotide sequence and amino acid sequence of IL-2 gene(sequence number:DQ852342) were 99.6% and 100% respectively;compared with other mammals in Genbank,including dog,cat,human,pig,cow,horse,goat,giant panda,rat,and so on,the homology of nucleotide sequence was between 64.8%(Rat) and 86.0%(Human),the homology of amino acid sequence was between 55.7%(Rat) and 80.1%(Human);③Compared with one published sequence(sequence number:AF169169),the homology of nucleotide sequence and amino acid sequence of IL-4 gene(sequence number:DQ852343) were both 100%;compared with other mammals in Genbank,including dog,cat,human,pig,cow,horse,goat,giant panda,rat,and so on,the homology of nucleotide sequence was between 57.0%(Giant panda) and 69.8%(Human),the homology of amino acid sequence was between 43.0%(Rat) and 53.6%(Human). Phylogenetic analysis based on the nucleotide sequences of the IFN-γ,IL-2 and IL-4 gene respectively showed that rabbit had more closed genetic relation with human,but the farthest with rat.It was consistent with the orthodox taxonomic results,rabbit and rat were belonged to Lagomorpha and Rodentia respectively.
Heme Deficiency is Associated with Senescence and Causes Suppression of NMDA Receptor Subunits Expression in

Heme is a crucial component of many pharmacological and toxicological processes and studies have suggested that heme deficiency may play a role in cellular ageing. A model of ageing neurons was established using prolonged cultures of BALB/c mouse primary cortical neurons. Aged neurons displayed a senescent phenotype and a marked up-regulation of cathepsin-L expression. Down-regulation of the candidate neuron-specific genes for Nmethyl-D-aspartate (NMDA) receptor subunits (NMDAζ1 and ε2) and neurofilament light peptide (NF-L) were found to be characteristic of the aging process as reported in vivo (Magnusson et al., 2002; Ossowska et al., 2001). In contrast, the genes for the controlling enzymes of heme synthesis and degradation (5-aminolevulinate synthase 1 and heme oxygenase 1 respectively) were up-regulated implicating depletion of a regulatory heme pool. Inhibition of heme synthesis (by 70-80%) at different enzymic steps by succinyl acetone (SA) and N-methylprotoporphyrin IX (NMP) resulted in the earlier lowered expression of NMDAζ1 and ε2 and NF-L. Exogenous hemin added to heme-depleted cells rescued the expression of these neuron-specific genes. Culture of cortical neurons from BALB/c Fech mutant mice demonstrating depressed heme synthesis showed premature senescence and reduced expression of NMDAζ1 and ε2 receptor subunits and NF-L compared with wild type cells. Our findings suggest that reduced availability of heme in neurons associated with senescence may have significant effects on synaptic function. This article has not been copyedited and formatted. The final version may differ from this version. Molecular Pharmacology Fast Forward. Published on November 23, 2005 as DOI: 10.1124/mol.105. 016675 at A PE T Jornals on Jauary 6, 2021 m oharm . aspeurnals.org D ow nladed from