Miracle-dz/UniFAR-FTUs huggingface dataset:
AZFa candidate gene UTY and its X homologue UTX are expressed in human germ cells

Summary The Ubiquitous Transcribed Y (UTY a.k.a. KDM6C) AZFa candidate gene on the human Y chromosome and its paralog on the X chromosome, UTX (a.k.a. KDM6A), encode a histone lysine demethylase removing chromatin H3K27 methylation marks at genes transcriptional start sites for activation. Both proteins harbour the conserved Jumonji C (JmjC) domain, functional in chromatin metabolism, and an extended N-terminal tetratricopeptide repeat (TPR) block involved in specific protein interactions. Specific antisera for human UTY and UTX proteins were developed to distinguish the expression of both proteins in human germ cells by immunohistochemical experiments on appropriate tissue sections. In the male germ line, UTY was expressed in the fraction of A spermatogonia located at the basal membrane, probably including spermatogonia stem cells. UTX expression was more spread in all spermatogonia and in early spermatids. In female germ line, UTX expression was found in the primordial germ cells of the ovary. UTY was also expressed during fetal male germ cell development, whereas UTX expression was visible only at distinct gestation weeks. Based on these results and the conserved neighboured location of UTY and DDX3Y in Yq11 found in mammals of distinct lineages, we conclude that UTY, such as DDX3Y, is part of the Azoospermia factor a (AZFa) locus functioning in human spermatogonia to support the balance of their proliferation-differentiation rate before meiosis. Comparable UTY and DDX3Y expression was also found in gonadoblastoma and dysgerminoma cells found in germ cell nests of the dysgenetic gonads of individuals with disorders of sexual development and a Y chromosome in karyotype (DSD-XY). This confirms that AZFa overlaps with GBY, the Gonadoblastoma susceptibility Y locus, and includes the UTY gene. Lay summary AZFa Y genes are involved in human male germ cells development and support gonadoblastoma (germ cell tumour precursor cells) in the aberrant germ cells of the gonads of females with genetic disorders of sexual development. The AZFa UTY gene on the male Y chromosome is equivalent to UTX on the female X chromosome. These genes are involved in removing gene regulators to enable activation of other genes (i.e. removal of histone methylation known as epigenetic modifications). We wanted to learn the function of UTY and UTX in developing sperm and eggs in human tissues and developed specific antibodies to detect both proteins made by these genes. Both UTY and UTX proteins were detected in adult and fetal sperm precursor cells (spermatogonia). UTX was detected in egg precursor cells (primordial germ cells). UTY was detected in gonadoblastoma and dysgerminoma tumour cells (germ cell tumours originating from genetic disorders of sexual development due to having a Y chromosome). Based on our study, we conclude that UTY is not only part of AZFa, but also of GBY the overlapping gonadoblastoma susceptibility Y region.
DOES THE SENSE OF RELATIONAL ENTITLEMENT MEDIATE THE ASSOCIATION BETWEEN NARCISSISM AND COUPLE CONFLICT?

Previous literature pointed out that narcissism affects the quality of interpersonal relationships. It has a negative impact on the functioning of the romantic dyads, determining higher levels of conflict and the use of maladaptive conflict resolution styles. However, the mechanisms linking narcissism and couple conflict are not sufficiently explored. This study expands the literature by examining the mediating role of the sense of relational entitlement. Participants in this study were 493 individuals (52.1 % women, M age = 22.39 years old) who were in a romantic relationship during the study (M relationship length = 26 months). The results indicated that some forms of relational entitlement mediated the link between narcissism and couple conflict. A person’s narcissism was related to their level of couple conflict, excessive and assertive entitlement. Both types of entitlement were related to conflict but in opposite directions. Higher excessive entitlement was associated with higher conflict, while higher assertive entitlement was associated with lower conflict. The indirect effects through both types of entitlement were significant. Restricted entitlement was not associated with narcissism or couple conflict. This study showed that although narcissism can lead to higher levels of relational entitlement and conflict, not all forms of entitlement negatively contributed to couple conflict.
Evaluation of SARS-CoV-2 spike antibody levels among Sputnik V first dose vaccinated people in Pakistan: formulation of national anti-COVID-19 mass vaccination strategy.

Rapid ramp up of immune responses against SARS-CoV-2 during pandemic enables adequate prevention and treatment for COVID-19. Estimating levels of SARS-CoV-2 spike protein antibodies post vaccination is crucial for designing mass-vaccination strategies. The aim of this study was to evaluate effectiveness of Sputnik V first dose in Pakistan. A cross-sectional study of 2000 participants was conducted for examining Gam-COVID-Vac or Sputnik V first dose effects at 21st days post administration at Islamabad Diagnostic Center, Islamabad, Pakistan. From 100 real-time PCR negative (SARS-CoV-2 RNA) individuals, samples were collected and analyzed for antibodies to the SARS-CoV-2 spike protein using Electro-chemiluminescence immunoassay (ECLIA) (Elecsys # 09289267190 Roche, USA). 85% of the participants showed strong positive results with SARS-CoV-2 spike protein antibodies > 1.5 AU/ml. The individuals with antibody titer > 250 AU/ml were 34.9%. Among those with antibody levels > 250 AU/ml 52% had previous history of COVID-19 infection. While participants with > 100 AU/ml of antibodies were 12.7%. However 9.5% showed antibody titer of > 25 AU/ml. 27% of participants had antibody titers of > 1.5–2.5 AU/ml. While antibody titers of < 1.5 AU/ml were observed among 15.9% of participants. Majority of the individuals represented significantly high antibody titers against SARS-CoV-2 even before second booster dose of Ad5 based Sputnik V vaccine. Continuous monitoring of antibody levels among COVID-19 vaccinated populations are deemed to assess humoral immunity status against SARS-CoV-2 infections.
Assessing the impediments to e-learning utilization by higher institution students

This study assessed the impediments to e-learning utilization by higher institution students in Katsina State, Nigeria. Survey type of descriptive research design was used in the study. There were 381 students randomly selected in four higher institutions. A questionnaire with test-retest reliability coefficient of 0.88 was used to gather data from the respondents. The researchers used Frequencies, percentages, means and t-test to analyze the data collected for the study. Findings revealed that all the fifteen impediments investigated in this study affect the e-learning utilization by higher institution students in Katsina State, Nigeria in varying degrees. The foremost factor is money to purchase e-learning resources followed by limited electricity supply. Moreover, significant difference was not found in the impediments to e-learning utilization by higher institution students on the basis of gender (t=-.796, Df=397, P>0.05) but significant difference was found in the factors affecting the use of e-learning by students’ of Universities and Colleges of Education (t=-2.969, P<0.05). It was recommended that all the identified impediments affecting the e-learning utilization by higher institution students in Katsina State, Nigeria should be addressed by the government.
Developing Family Satisfaction with Care in Adult Critical Care Public Hospital Terengganu, Malaysia

In admissions to the intensive care unit (ICU), there is a high possibility of a life-threatening condition and possible emotional distress for family members. When the family is distressed and hospitalized, a significant level of stress and anxiety will be generated among family members, thereby decreasing their ability to make responsible decisions. As a result, the family members need full and up-to-date details, helping them to retain hope, and this contributes to lower stress levels. While there is growing evidence of the effectiveness of shared decision-making for family members who are directly involved in decisions, particularly regarding shared decision-making in the Malaysian context, there is less evidence that supported decisions help overall outcome. This study aims to developing the family satisfaction with decision making in the Intensive Care Unit (FS-ICU)-33 Malay language version of family member’s satisfaction with care and decision making during their stay at the intensive care units. A quantitative, cross-sectional validation study and purposive sampling was conducted from 1st November 2017 and 10 October 2018 to January 2020 among 208 of family members. The family members of the ICU patients involved in this study had an excellent satisfaction level with service care. Higher satisfaction in ICU care resulting in higher decision-making satisfaction and vice versa.
Evaluation of the Demand-Driven Agricultural Extension Curricula among Alumni from Selected Universities in Nigeria

The study assessed demand-driven agricultural extension curricula by alumni from universities in Nigeria partnering with Sasakawa Africa Fund for Extension Education (SAFE) on such curriculum for at least seven years. Using a cross-sectional research design and cluster sampling technique, 227 alumni were randomly selected from a population of 520. Data were collected through structured questionnaire on socio-economic characteristics, mode of study, areas of specialization operationalized as close-ended questions and rating of programme components on a 5 -point Likert type scale of poor (1) fair (2) good (3) very good (4) excellent (5) with 70 items. Data entry and analysis were done with SPSS 25 using percentages, mean score and multiple regression analysis. Adult education approaches, supervised enterprise projects, students’ supervision, academic advice, information sharing, and participation in research projects had highest positive rating; while fund raising, scholarship, opportunity for enrolling and be part of a professional scientific network, linkage & involvement with Sasakawa Africa Association (SAA) field activities, laboratories and variety of subjects offered had highest negative rating. Socio-economic characteristics of alumni were significantly correlated and explained 49 percent of the variation in programme rating. The study recommends that there is need to review the curricula covered in this study and mechanisms for compliance with the Sasakawa methodology should be reinvigorated. Keywords: alumni, demand-driven, agricultural extension, university curricula.
Subjective Well-being, Income, and Ethnicity in Slovakia

ABSTRACT This paper utilizes two measures of subjective well-being to test a hypothesis that a marginal increase in subjective well-being associated with a marginal increase in income is larger for poorer than for richer populations. This hypothesis is examined in the setting of Slovak Roma, who are poor in comparison to the non-Roma population. The results suggest that the correlation between income and satisfaction is greater for the lower-income group (the Roma) than for the higher-income group (majority population). However, the correlation between income and emotional well-being does not significantly differ between the two groups.
The Effect of Business Literation on SMEs Performance with Mediation of Risk Management and Entrepreneurial Self Efficacy

Literacy is the main prerequisite for intellectual growth and the ability to learn to generate new knowledge. This study aims to analyze the effect of business literacy on the performance of SMEs, either directly or indirectly by including the Entrepreneurial Self-Efficacy (ESE) and Enterprise Risk Management (ERM) variables as mediation and moderation variables, respectively. The approach of this article is thought or conceptual based on intellectualization and reflects the prospects and impact of digital marketing. This study uses a critical system to analytically review digital marketing literature and relate it to research results. The data analysis technique used is descriptive. Based on the results of tracking and analysis, there has never been any previous research that examines business literacy, ESE, ERM, and SME performance in one model. The implication of social justice in the field of business literacy is through the existence of a business application system that is socialized to business people. Business information can enhance or even suppress the effects of entrepreneurial self-efficacy. ERM as a planned action is a control activity, which can be perceived not as an obstacle to gaining profits, but to get the expected performance results.
Antimicrobial, Antioxidant and Antiproliferative Properties of the Leaves of Senna siamea

The aim of this study was to investigate the antimicrobial, antioxidant and antiproliferative activities of leaves of Senna siamea. A sequential solvent extraction procedure was used in extracting powdered Senna leaves, starting with hexane, ethylacetate, ethanol and distilled water, respectively. Dry extracts obtained were tested at various concentrations against Pseudomonas aeruginosa, staphylococcus aureus, Salmonella typhi and Candida albicans. Antioxidant (Qualitative and quantitative) and antiproliferative tests were carried out on samples of the dry extracts. Ethylacetate extract of Senna siamea showed the highest antibacterial activity against S. typhi (zone of inhibition 15.0 mm) at concentration of 500mg/ml. The aqueous extract had the highest antioxidant activity, evidenced by a low IC50 value of 12. 89µg/ml. Antiproliferative activity was determined by calculating the percentage inhibition of germination of Sorghum bicolor L. seeds under appropriate conditions. Plants that inhibit seed germination may inhibit tumour growth. At 24 hours, the plant showed strong antiproliferative activity as evidenced by the high percentage inhibition. Ethanolic extract had the highest antiproliferative activity (90%) at 5000µg/ml, followed by the aqueous extract (88.5%). However, at 48 hours, all organic extracts showed very weak antiproliferative activity as evidenced by the low % inhibition. Only the aqueous extract showed considerable antiproliferative activity at 5000µg/ml (45%). This implies that the aqueous extract has considerable antiproliferative activity, hence, it may be a promising anticancer drug or has components that have anticancer properties. This shows that Senna leaves could serve as source of bioactive compounds with potential antimicrobial, antioxidant and anticancer properties.
Modelling Selectional Super-Flexibility

The selectional ﬂexibility of some attitude verbs (e.g. know , realize , report ) between declarative and interrogative complements has been the subject of much recent work in formal semantics. However, little attention has been paid to verbs (e.g. see , remember , observe ) that embed an even wider variety of complements (incl. subject-controlled gerundive small clauses and concrete object-denoting DPs). Since the familiar types of some of these complements resist an embedding in the type for questions [ = sets of propositions], these verbs challenge Theiler, Roelofsen & Aloni’s (2018) uniform interpretation strategy for the complements of declarative/interrogative-neutral verbs. The present paper answers this challenge by uniformly interpreting the different complements of selectionally super-ﬂexible verbs like remember in a generalized type for questions, viz. as parametrized centered questions . It shows that the resulting semantics captures the intuitive entailment pattern of these verbs.
Low-level overexpression of wild type TDP-43 causes late-onset, progressive neurodegeneration and paralysis in mice

Modestly increased expression of transactive response DNA binding protein (TDP-43) gene have been reported in amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and other neuromuscular diseases. However, whether this modest elevation triggers neurodegeneration is not known. Although high levels of TDP-43 overexpression have been modeled in mice and shown to cause early death, models with low-level overexpression that mimic the human condition have not been established. In this study, transgenic mice overexpressing wild type TDP-43 at less than 60% above the endogenous CNS levels were constructed, and their phenotypes analyzed by a variety of techniques, including biochemical, molecular, histological, behavioral techniques and electromyography. The TDP-43 transgene was expressed in neurons, astrocytes, and oligodendrocytes in the cortex and predominantly in astrocytes and oligodendrocytes in the spinal cord. The mice developed a reproducible progressive weakness ending in paralysis in mid-life. Detailed analysis showed ∼30% loss of large pyramidal neurons in the layer V motor cortex; in the spinal cord, severe demyelination was accompanied by oligodendrocyte injury, protein aggregation, astrogliosis and microgliosis, and elevation of neuroinflammation. Surprisingly, there was no loss of lower motor neurons in the lumbar spinal cord despite the complete paralysis of the hindlimbs. However, denervation was detected at the neuromuscular junction. These results demonstrate that low-level TDP-43 overexpression can cause diverse aspects of ALS, including late-onset and progressive motor dysfunction, neuroinflammation, and neurodegeneration. Our findings suggest that persistent modest elevations in TDP-43 expression can lead to ALS and other neurological disorders involving TDP-43 proteinopathy. Because of the predictable and progressive clinical paralytic phenotype, this transgenic mouse model will be useful in preclinical trial of therapeutics targeting neurological disorders associated with elevated levels of TDP-43.
Data augmentation with occluded facial features for age and gender estimation

Funding Information Ministry of Science and Technology; 106‐2221‐E‐011‐153‐MY2 Abstract Here, the feature occlusion, a data augmentation method that simulates real‐life challenges on the main features of the human face for age and gender recognition is proposed. Previous methods achieved promising results on constrained data sets with strict environmental settings, but the results on unconstrained data sets are still far from perfect. The proposed method adopted three simple occlusion techniques, blackout, random brightness, and blur, and each simulates a different kind of challenge that would be encountered in real‐world applications. A modified cross‐entropy loss that gives less penalty to the age predictions that land on the adjacent classes of the ground truth class is also proposed. The effectiveness of our proposed method is verified by implementing the augmentation method and modified cross‐entropy loss on two different convolution neural networks, the slightly modified AdienceNet and the slightly modified VGG16, to perform age and gender classification. The proposed augmentation system improves the age and gender classification accuracy of the slightly modified AdienceNet network by 6.62% and 6.53% on the Adience data set, respectively. The proposed augmentation system also improves the age and gender classification accuracy of the slightly modified VGG16 network by 6.20% and 6.31% on the Adience data set, respectively.
School burnout among Chinese high school students: the role of teacher-student relationships and personal resources

Abstract This study addresses the high level of academic demands and workload in Chinese high schools and aims to identify protective factors against students’ negative emotional responses. Using the well-established Job Demands-Resources Model as a guiding framework, we investigated the relationship between workload, perceived academic demands, teacher-student relationships, personal resources (self-efficacy, self-esteem, and optimism), and school burnout symptoms (emotional exhaustion and cynicism). Data analyses were based on self-reports from 1,083 Chinese students. The results of structural equation modelling showed that workload and academic demands were positively related to burnout. Among the resources, optimism was negatively related to emotional exhaustion and cynicism, whereas self-efficacy was only negatively related to cynicism. Surprisingly, self-esteem was positively related to cynicism after controlling for the other two personal resources. Our findings underline the potentially protective effect of students’ self-efficacy, dispositional optimism, and self-esteem (at least at the bivariate level) against school burnout.
Influence of competitive intensity on stadium attendance. An analysis of the French football Ligue 1 over the period 2009-2019 through a visualization system

ABSTRACT The relationship between competitive balance or intensity and venue attendance has been extensively tested in the literature. These studies have used complex metrics and econometric testing. This does not favour their understanding and application by managers. This article investigates, over a 10 years period (2009–2019), the influence of competitive intensity on stadium attendance for the French men’s football Ligue 1 through a visualization approach. The use of this method provides visual information via a single chart per season about the influence of competitive intensity on attendance, depending on the moment in the season and the sporting stakes. This means that managers can look at relevant information in one single chart. The article shows the positive influence on attendance of competitive intensity in relation to the different sporting stakes, consistent with previous studies. The visualization approach makes the results easy to grasp for managers, facilitating the identification of managerial implications.