Miracle-dz/UniFAR-FTUs huggingface dataset:
A roadmap for sole Cherenkov radiators with SiPMs in TOF-PET

Time of flight positron emission tomography can strongly benefit from a very accurate time estimator given by Cherenkov radiation, which is produced upon a 511 keV positron–electron annihilation gamma interaction in heavy inorganic scintillators. While time resolution in the order of 30 ps full width at half maximum (FWHM) has been reported using MCP-PMTs and black painted Cherenkov radiators, such solutions have several disadvantages, like high cost and low detection efficiency of nowadays available MCP-PMTs. On the other hand, silicon photomultipliers (SiPMs) are not limited by those obstacles and provide high photon detection efficiency with a decent time response. Timing performance of PbF2 crystals of various lengths and surface conditions coupled to SiPMs was evaluated against a reference detector with an optimized test setup using high-frequency readout and novel time walk correction, with special attention on the intrinsic limits for one detected Cherenkov photon only. The average number of detected Cherenkov photons largely depends on the crystal surface state, resulting in a tradeoff between low photon time spread, thus good timing performance, and sensitivity. An intrinsic Cherenkov photon yield of 16.5 ± 3.3 was calculated for 2 × 2 × 3 mm3 sized PbF2 crystals upon 511 keV γ-deposition. After time walk correction based on the slew rate of the signal, assuming two identical detector arms in coincidence, and using all events, a time resolution of 215 ps FWHM (142 ps FWHM) was obtained for 2 × 2 × 20 mm3 (2 × 2 × 3 mm3) sized PbF2 crystals, compared to 261 ps (190 ps) without correction. Selecting on one detected photon only, a single photon coincidence time resolution of 113 ps FWHM for black painted and 166 ps for Teflon wrapped crystals was measured for 3 mm length, compared to 145 ps (black) and 263 ps (Teflon) for 20 mm length.
Maternal blood pressure trajectories and associations with gestational age at birth: a functional data analytic approach

Supplemental Digital Content is available in the text Background: Research has revealed group-level differences in maternal blood pressure trajectories across pregnancy. These trajectories are typically constructed using clinical blood pressure data and multivariate statistical methods that are prone to bias and ignore the functional, dynamic process underlying a single blood pressure observation. The aim of this study was to use functional data analysis to explore blood pressure variation across pregnancy, and multivariate methods to examine whether trajectories are related to gestational age at birth. Methods: Clinical blood pressure observations were available from 370 women who participated in a longitudinal pregnancy cohort study conducted in Montreal, Quebec, Canada. Functional data analysis was used to smooth blood pressure data and then to conduct a functional principal component analysis to examine predominant modes of variation. Results: Three eigenfunctions explained greater than 95% of the total variance in blood pressure. The first accounted for approximately 80% of the variance and was characterized by a prolonged-decrease trajectory in blood pressure; the second explained 10% of the variance and captured a late-increase trajectory; and the third accounted for approximately 7% of the variance and captured a mid-decrease trajectory. The prolonged-decrease trajectory of blood pressure was associated with older, and late-increase with younger gestational age at birth. Conclusion: Functional data analysis is a useful method to model repeated maternal blood pressure observations and many other time-related cardiovascular processes. Results add to previous research investigating blood pressure trajectories across pregnancy through identification of additional, potentially clinically important modes of variation that are associated with gestational age at birth.
GIS-Based Analysis Framework to Identify the Determinants of COVID-19 Incidence and Fatality in Africa

Corona virus diseases 2019 (COVID-19) pandemic is an extraordinary threat with significant implications in all aspects of human life; therefore, it represents the most immediate challenge for the countries all over the world. This study, hence, is intended to identify the best GIS-based model that can explore, quantify, and model the determinants of COVID-19 incidence and fatality. For this purpose, geospatial models were developed to estimate COVID-19 incidence and fatality rates in Africa, up to 16th of August 2020 at the national level. The models involved Ordinary Least Squares (OLS) and Geographically Weighted Regression (GWR) analysis using ArcGIS. Spatial autocorrelation analysis recorded a positive spatial autocorrelation in COVID-19 incidence (Moran index 0.16, P = 0.1) and fatality (Moran index 0.26, P = 0.01) rates within different African countries. GWR model had higher R2 than OLS for prediction of incidence and mortality (58% vs 45% and 55% vs 53%). The main predictors of COVID-19 incidence rate were overcrowding, health expenditure, HIV infections, air pollution, and BCG vaccination (mean β = 3.10, 1.66, 0.01, 3.79, and −66.60 respectively, P < 0.05). The main determinants of COVID-19 fatality were prevalence of bronchial asthma, tobacco use, poverty, aging, and cardiovascular diseases fatality (mean β = 0.00162, 0.00004, −0.00025, −0.00144, and −0.00027 respectively, P < 0.05). Application of the suggested model can assist in guiding intervention strategies, particularly at the local and community level whenever the data on COVID-19 cases and predictors variables are available.
SLC-30A9 is required for Zn2+ homeostasis, Zn2+ mobilization, and mitochondrial health

Significance Zinc plays important roles in numerous cellular processes. Deficiency or excess of Zn2+ leads to many diseases. Zn2+ concentration at various cellular compartments is regulated. Imbalance of Zn2+ in mitochondria has been linked to neurodegeneration. However, little is known about how mitochondrial Zn2+ is regulated. We find that SLC-30A9 is required for Zn2+ export from mitochondria in both Caenorhabditis elegans and human cells. Loss of slc-30a9 leads to excessive Zn2+ accumulation in mitochondria, severe mitochondrial swelling, compromised mitochondrial metabolic function, reductive stress, and induction of the mitochondrial stress response. SLC-30A9 is also essential for organismal fertility and sperm activation. In neurons, slc-30a9 mutations cause dramatically reduced mitochondria in neurites, providing a potential mechanism for the Birk–Landau–Perez cerebrorenal syndrome. The trace element zinc is essential for many aspects of physiology. The mitochondrion is a major Zn2+ store, and excessive mitochondrial Zn2+ is linked to neurodegeneration. How mitochondria maintain their Zn2+ homeostasis is unknown. Here, we find that the SLC-30A9 transporter localizes on mitochondria and is required for export of Zn2+ from mitochondria in both Caenorhabditis elegans and human cells. Loss of slc-30a9 leads to elevated Zn2+ levels in mitochondria, a severely swollen mitochondrial matrix in many tissues, compromised mitochondrial metabolic function, reductive stress, and induction of the mitochondrial stress response. SLC-30A9 is also essential for organismal fertility and sperm activation in C. elegans, during which Zn2+ exits from mitochondria and acts as an activation signal. In slc-30a9–deficient neurons, misshapen mitochondria show reduced distribution in axons and dendrites, providing a potential mechanism for the Birk–Landau–Perez cerebrorenal syndrome where an SLC30A9 mutation was found.
A comprehensive transcriptomic analysis of alternate interferon signaling pathways in peripheral blood mononuclear cells in rheumatoid arthritis

Interferon (IFN) signaling pathways play crucial roles in the pathogenesis of rheumatoid arthritis (RA). Prior studies have mainly studied mixed alterations in the IFN signaling pathway in RA, but these studies have not been sufficient to elucidate how imbalanced IFN signaling subtly influences immune cells. Single-cell RNA (scRNA) sequencing makes it possible to better understand the alternations in the interferon signaling pathways in RA. In the present study, we found that IFN signaling pathways were activated in natural killer (NK) cells, monocytes, T cells, B cells, and most immune cell subclasses in RA. We then explored and analyzed the connections between abnormal IFN signaling pathways and cellular functional changes in RA. Single-Cell rEgulatory Network Inference and Clustering (SCENIC) analysis and gene regulatory network (GRN) construction were also performed to identify key transcription factors in RA. Finally, we also investigated altered IFN signaling pathways in multiple RA peripheral blood samples, which indicated that abnormal IFN signaling pathways were universally observed in RA. Our study contributes to a better understanding of the delicate and precise regulation of IFN signaling in the immune system in RA. Furthermore, common alternations in IFN signaling pathway-related transcription factors could help to identify novel therapeutic targets for RA treatment.
Isolation and Characterization of Nickel-Tolerant Trichoderma Strains from Marine and Terrestrial Environments

Nickel contamination is a serious environmental issue that requires immediate action. In this study, 23 strains of Trichoderma were isolated from terrestrial and marine environments and identified using a polyphasic approach of morphological characterization and ITS gene sequence analysis. The Trichoderma strains were tested for their tolerance and biosorption of nickel. Our results showed the growth of all Trichoderma strains on Trichoderma Selective Medium (TSM) with 50–1200-ppm nickel, indicating their tolerance of this heavy metal even at a relatively high concentration. Six Trichoderma strains (three isolated from terrestrial substrates and three from marine substates) had the highest radial growth on TSM with 50-ppm Ni. Among these fungal isolates, Trichoderma asperellum (S03) isolated from soil exhibited the best growth after 2 days of incubation. For the biosorption of nickel, the accumulation or uptake efficiency by the six selected Trichoderma was determined in Potato Dextrose Broth (PDB) supplemented with 50-ppm Ni using a Flame Atomic Absorption Spectrophotometer (AAS). The percent uptake efficiency of the three strains of T. asperellum (S03, S08, and LL14) was computed to be up to 66%, while Trichoderma virens (SG18 and SF22) and Trichoderma inhamatum (MW25) achieved up to 68% uptake efficiency. Observation of the Trichoderma strains with Scanning Electron Microscopy (SEM) before and after the absorption of nickel showed very minimal damage on the hyphal and conidial surface morphology, but changes in the colonial characteristics were observed. Our study highlighted the potential of terrestrial and marine strains of Trichoderma for the bioremediation of nickel pollution.
Longitudinal Outcomes in Adolescents After Referral for Metabolic and Bariatric Surgery

Supplemental Digital Content is available in the text ABSTRACT Background: Metabolic and bariatric surgery (MBS) can be a well tolerated and effective treatment option for severe obesity in adolescents. We compared outcomes for adolescents that did and did not proceed to surgery. Methods: A single-center longitudinal study (2015–2020). Patients were identified as LSG if they completed laparoscopic sleeve gastrectomy within 6 months of initial visit and NoLSG if they did not. Chi-square, Fisher exact, nonparametric Kruskal-Wallis tests, and Linear Mixed Models (LMM) were used to compare outcomes over 2 years. Results: Three hundred fifty-two adolescents were referred with a mean age of 15.6 ± 1.4, 69% girls, 38% Hispanic, and 78% had noncommercial insurance. The median baseline weight was 135 kg and body mass index (BMI) was 48 kg/m2; 42% had a BMI >50. Seventy-nine (22%) underwent LSG whereas 273 (78%) did not complete MBS primarily because of lack of interest. LSG patients had 21% total weight loss and 22% total BMI loss at 24 months whereas NoLSG patients had 4% total weight gain and 3% BMI gain (P < 0.01). Obesity-associated conditions improved in the LSG group (P < 0.01). Follow-up in both groups was poor (≤30% at 24 months). Patients with public insurance and those with BMI from 50 to 59.9 kg/m2 were high performing LSG patients. Conclusions: A minority (22%) of adolescents referred for MBS proceeded to surgery, despite its demonstrated efficacy and safety in adolescence. Those that did not undergo surgery continued to gain weight. Further research is needed to understand patient preferences or concerns related to MBS utilization during adolescence.
Normative intercapillary distance and vessel density data in the temporal retina assessed by wide-field spectral-domain optical coherence tomography angiography

A limitation of conventional optical coherence tomography angiography (OCTA) is the limited field of view normally used in data acquisition. As the technology improves, larger fields of view that capture information away from the macular are being explored in order to provide an enhanced ability to detect pathology. However, normative measurements for important OCTA metrics like vessel density and intercapillary distance are not currently well-characterized in the peripheral retina. In this prospective study, we measured vessel density and intercapillary distance of the superficial vascular complex, ganglion cell layer plexus, and deep capillary plexus in montaged macular/temporal scans from 53 (33 men) healthy volunteers. Vessel density and intercapillary distance were also compared across different regions of the retina, including along arcs at separate distance from the fovea. Compared to the central macular region, the temporal retina had significantly lower vessel density, decreased thickness, and greater intercapillary distance in the superficial vascular complex, GCLP ganglion cell layer plexus, and deep capillary plexus (Wilcoxon rank sum test P < 0.001), with each of the plexuses examined here showing a general decrease in vessel density and an increase in intercapillary distance towards the temporal region. No significant difference was noted comparing corresponding vessel density and intercapillary distance regions above and below the macula, and multiple linear regression showed that age and intraocular pressure were not associated with vessel density and intercapillary distance in most models. Repeatability analysis reported as intraclass correlation coefficients demonstrated moderate to excellent reliability of vessel density and intercapillary distance in all OCTA layers. These results should help provide an enhanced baseline to help identify vascular pathology in the peripheral retina.
Effects of Different Exercise Interventions on Cardiac Autonomic Control and Secondary Health Factors in Middle-Aged Adults: A Systematic Review

This systematic review was conducted in accordance with the PRISMA guidelines to summarize the existing literature on the effects of different exercise interventions on cardiac autonomic control and secondary health factors. Resting heart rate variability (HRV) was used as indicator of cardiac autonomic control. Secondary factors were related to factors that contribute to cardiovascular health. Studies examining the effects of endurance, resistance, multimodal, or coordinative training interventions in healthy participants aged between 45 and 60 years old on average were considered. The methodological quality of the studies was examined using two assessment scales (TESTEX and STARDHRV). PROSPERO registration number: CRD42020206606. The literature review retrieved eight studies fulfilling all inclusion criteria. Cardiac autonomic control and cardiovascular health improved after endurance and multimodal interventions. Resistance training had no significant impact on HRV or any secondary health factor. Coordinative exercise interventions showed inconclusive results regarding HRV but showed significant improvements in secondary health factors. The quality assessment tools revealed some methodological and reporting deficits. Despite the small number of studies, we suggest endurance and multimodal interventions including aerobic exercises for the enhancement of cardiac autonomic control and the reduction of cardiovascular risk in middle-aged adults. Further studies need to be conducted to examine the long-term effects of exercise in the midlife period.
Personalized approach for type 2 diabetes pharmacotherapy: where are we and where do we need to be?

ABSTRACT Introduction Cluster analysis has identified distinct groups of type 2 diabetes (T2D) subjects with distinct metabolic characteristics. Thus, personalizing pharmacologic therapy to individual phenotypic and pathophysiologic characteristics has potential to improve metabolic control and reduce risk of microvascular and macrovascular complications. Areas covered The authors review the classification of T2D, genetic markers, pathophysiology and natural history of T2D, the ABCDE approach to therapy, the ADA/EASD stepwise approach to therapy, available antidiabetic agents, and provide a more rational therapeutic approach based upon pathophysiology and cardiovascular and renal outcome trials. Expert opinion Although insulin resistance is the earliest detectable abnormality, overt T2D does not occur in the absence of progressive beta cell failure. Because of the complex etiology of T2D (Ominous Octet), initiation of therapy with combined agents that (i) target both insulin resistance and beta cell dysfunction and (ii) prevent macrovascular, as well as microvascular, complications will be required. The ratio of C-peptide at 120 minutes (OGTT) to baseline C-peptide predicts with high sensitivity who will respond to metformin, the response to glucose-lowering agents and provides a useful tool to guide optimal glucose lowering therapy.
Antioxidant Activity of Phenolic and Alkaloid Fractions Accumulated in Artemisia Judaica and Artemisia Herba Alba

Genus Artemisia are desert plants spread in the Egypt and Saudi Arabia. The methanol extract of the aerial parts of Artemisia herba alba and Artemisia judaica grown in the arid zones in the Northern regions of Saudi Arabia and North Sinai, in Egypt were subjected to the primary phytochemical analysis which, showed the presence of phenolic compounds, flavonoids, tannins and sterols. The GC-MS analysis of the methanolic extract exhibited numerous compounds, 11 compounds were identified as phenolic compounds, ferulic acid, ursodeoxycholic acid and derivatives of cinnamic acid (ethyl cinnamate). The highest phenolics content in A.herba alba grown in Saudi Arabia was observed for methyl cinnamate, (8.2 %) and the lowest methyl ferulate (0.02 %) for A. judica grown in Egypt. The quantization analysis of GC-MS revealed high amount of phenolic acid in the plant grow in Saudi Arabia than the plant collected from Egypt. The radical scavenging activities of two plant were studied by 1,1-diphenyl -2-pycrylhydrazyl (DPPH), EC (50) values were determined for A.herba alba and A.judaica (0.122, and 0.56 mg/ml) growing in Saudi Arabia and A.herba alba and A. judaica (1.7 and 0. 67 mg/ml ) growing in Egypt. Total alkaloid content was analysed and some alkaloid compounds was identified by GC-MS, viz. dasycarpidan-1-methanol, acetate, pseudosolasodine diacetate and morphinan4,5epoxy3,6-diol. Certain compounds were rich in Saudi plant but not detected in plants grown in Egypt, which reflect the role of environment adaptation of plant by accumulation of only certain compounds.
Assessment of Knowledge, Attitudes, and Practices Regarding Dengue among Physicians: A Web-Based Cross-Sectional Survey

Dengue fever is one of the most important viral infections transmitted by Aedes mosquitoes and a major cause of morbidity and mortality globally. Accurate identification of cases and treatment of dengue patients at the early stages can reduce medical complications and dengue mortality rate. This survey aims to determine the knowledge, attitude, and practices (KAP) among physicians in dengue diagnosis and treatment. This study was conducted among physicians in Turkey as one nonendemic country and Bangladesh, India, and Malaysia as three dengue-endemic countries. The dosing frequencies, maximum doses, and contraindications in dengue fever were examined. The results found that physicians from Bangladesh, India, and Malaysia have higher KAP scores in dengue diagnosis and treatment compared to physicians in Turkey. This may be due to a lack of physician’s exposure to a dengue patient as Turkey is considered a nonendemic country. This assessment may help establish a guideline for intervention strategies among physicians to have successful treatment outcomes and reduce dengue mortality.
New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia

The host lymphocyte response is decisive in Pneumocystis pneumonia (PCP) pathophysiology but little is known of the specific roles of lymphocyte subpopulations in this fungal infection. Peripheral NK, NKT, B, TCD4+ and TCD8+ subpopulations were compared by immunophenotyping between 20 patients diagnosed with PCP (PCP(+)] and 20 uninfected immunosuppressed patients (PCP(−)). Among PCP(+) subjects, the lymphocyte populations were also compared between surviving and deceased patients. Low B cell count (<40 cells/µL) was more frequent in PCP(+) than in PCP(−) patients (p = 0.03), while there was no difference for the TCD4 count. Among the PCP(+) group, the 7 deceased patients had lower Th1 (p = 0.02) and Tc1 (p = 0.03) populations, higher Th2 response (p = 0.03), higher effector TCD8 (p < 0.01), lower central memory TCD8 (p = 0.04) and reduced NK cells (p = 0.02) compared with the 13 survivors. Th1/Th2 ratio < 17, CD8 Tc1 < 44%, effector TCD8 < 25%, central memory TCD8 < 4%, NK cells < 50 cells/µL and total lymphocytes < 0.75 G/L were associated with a higher risk of mortality (p = 0.003, p = 0.007, p = 0.0007, p = 0.004, p = 0.02 and p = 0.019, respectively). The traditional analysis of TCD4 and TCD8 populations may be insufficient in the context of PCP. It could be completed by using B cells to predict the risk of PCP, and by using lymphocyte subpopulations or total lymphocyte count, which are easy to obtain in all health care facilities, to evaluate PCP prognosis.
Physicochemical properties of the dissolved organic carbon can lead to different physiological responses of zebrafish (Danio rerio) under neutral and acidic conditions.

Previous studies have suggested that the capacity of natural dissolved organic carbon (DOC) molecules to interact with biological membranes is associated with their aromaticity (SAC340 ); origin (allochthonous versus autochthonous, FI); molecular weight (Abs254/365 ); and relative fluorescence of DOC moieties (PARAFAC analysis). These interactions may be especially important when fish are challenged by acidic waters, which are known to inhibit the active uptake of Na+ and Cl- , while stimulating diffusive ion losses in freshwater fishes. Therefore, zebrafish were acclimated (7 days, pH 7.0) to five natural DOC sources (10 mg C/L), two from the Amazon Basin and three from Canada, together with a "no-added DOC" control. After the acclimation, fish were challenged by exposure to acidic water (pH 4.0) for 3 h. Osmoregulatory parameters were measured at pH 7.0 and 4.0. Acclimation to the five DOC sources did not disturb Na+ , Cl- and ammonia net fluxes, but resulted in differential elevations in Na+ , K+ ATPase and v-type H+ ATPase activities in fish at pH 7.0. However, after transfer to pH.4.0, the control fish exhibited rapid increases in both enzymes. In contrast the DOC- acclimated animals exhibited unchanged (Na+ , K+ ATPase) or differentially increased (v-type H+ ATPase) activities. Na+ , Cl- and ammonia net fluxes remained unchanged in the control fish, but were differentially elevated in most of the DOC treatments at pH 4.0, relative to the same DOC treatments at pH 7.0. Correlations between the osmoregulatory data the DOCs properties highlight that the DOC properties drive different effects on gill physiology.