Miracle-dz/UniFAR-FTUs huggingface dataset:
Deciphering the in vivo Dynamic Proteomics of Mesenchymal Stem Cells in Critical Limb Ischemia

Objective Regenerative therapy using mesenchymal stem cells (MSC) is a promising therapeutic method for critical limb ischemia (CLI). To understand how the cells are involved in the regenerative process of limb ischemia locally, we proposed a metabolic protein labeling method to label cell proteomes in situ and then decipher the proteome dynamics of MSCs in ischemic hind limb. Methods and Results In this study, we overexpressed mutant methionyl-tRNA synthetase (MetRS), which could utilize azidonorleucine (ANL) instead of methionine (Met) during protein synthesis in MSCs. Fluorescent non-canonical amino-acid tagging (FUNCAT) was performed to detect the utilization of ANL in mutant MSCs. Mice with hindlimb ischemia (HLI) or Sham surgery were treated with MetRSmut MSCs or PBS, followed by i.p. administration of ANL at days 0, 2 6, and 13 after surgery. FUNCAT was also performed in hindlimb tissue sections to demonstrate the incorporation of ANL in transplanted cells in situ. At days 1, 3, 7, and 14 after the surgery, laser doppler imaging were performed to detect the blood reperfusion of ischemic limbs. Ischemic tissues were also collected at these four time points for histological analysis including HE staining and vessel staining, and processed for click reaction based protein enrichment followed by mass spectrometry and bioinformatics analysis. The MetRSmut MSCs showed strong green signal in cell culture and in HLI muscles as well, indicating efficient incorporation of ANL in nascent protein synthesis. By 14 days post-treatment, MSCs significantly increased blood reperfusion and vessel density, while reducing inflammation in HLI model compared to PBS. Proteins enriched by click reaction were distinctive in the HLI group vs. the Sham group. 34, 31, 49, and 26 proteins were significantly up-regulated whereas 28, 32, 62, and 27 proteins were significantly down-regulated in HLI vs. Sham at days 1, 3, 7, and 14, respectively. The differentially expressed proteins were more pronounced in the pathways of apoptosis and energy metabolism. Conclusion In conclusion, mutant MetRS allows efficient and specific identification of dynamic cell proteomics in situ, which reflect the functions and adaptive changes of MSCs that may be leveraged to understand and improve stem cell therapy in critical limb ischemia.
Chlorogenic Acid Improves NAFLD by Regulating gut Microbiota and GLP-1

Our previous studies have shown that chlorogenic acid (CGA) could significantly improve acute and chronic liver injury through antioxidant and anti-inflammatory activities. However, its effect on non-alcoholic fatty liver disease (NAFLD) are not entirely clear. This study aims to explore the effect of CGA on NAFLD induced by high-fat diet (HFD) and whether it regulates the gut microbiota and Glucagon-like peptide-1 (GLP-1). NAFLD mice were established by HFD and treated with or without CGA. Serum transaminase, fasting blood glucose (FBG), blood lipids, insulin, GLP-1 and lipopolysaccharide (LPS) were detected. Liver histology was evaluated with Hematoxylin-eosin staining. Toll like receptor 4 (TLR4) signaling pathway was analyzed with western blot and inflammatory cytokines were detected with real-time PCR. The content of gut microbiota were determined with real-time PCR of the bacterial 16S rRNA gene. Expressions of intestine tight junctional protein were examined with immunohistochemistry. CGA could alleviate HFD-induced hepatic steatosis and inflammation, reduce serum transaminase, FBG and blood lipids, increase insulin sensitivity. CGA also could reverse HFD-induced activation of TLR4 signaling pathway and expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in liver. Meanwhile, CGA increased the content of Bifidobacterium and reduced the content of Escherichia coli in feces. Furthermore, CGA could increase the expression of tight junction proteins Occludin and zonula occludens-1 (ZO-1) in intestinal tissue. Moreover, CGA could the level of LPS and increased the level of GLP-1 in portal vein. These results indicated that CGA protected against HFD-induced hepatic steatosis and inflammation probably through its anti-inflammatory effects associated with regulation of gut microbiota and an increase of GLP-1 secretion and thus could be used as a potential drug for prevention and treatment of NAFLD.
Alterations of Growth and Focal Adhesion Molecules in Human Breast Cancer Cells Exposed to the Random Positioning Machine

In this study, we evaluated changes in focal adhesions (FAs) in two types of breast cancer cell (BCC) lines (differentiated MCF-7 and the triple-negative MDA-MB-231 cell line) exposed to simulated microgravity (s-μg) created by a random positioning machine (RPM) for 24 h. After exposure, the BCC changed their growth behavior and exhibited two phenotypes in RPM samples: one portion of the cells grew as a normal two-dimensional monolayer [adherent (AD) BCC], while the other portion formed three-dimensional (3D) multicellular spheroids (MCS). After 1 h and 30 min (MDA-MB-231) and 1 h 40 min (MCF-7), the MCS adhered completely to the slide flask bottom. After 2 h, MDA-MB-231 MCS cells started to migrate, and after 6 h, a large number of the cells had left the MCS and continued to grow in a scattered pattern, whereas MCF-7 cells were growing as a confluent monolayer after 6 h and 24 h. We investigated the genes associated with the cytoskeleton, the extracellular matrix and FAs. ACTB, TUBB, FN1, FAK1, and PXN gene expression patterns were not significantly changed in MDA-MB-231 cells, but we observed a down-regulation of LAMA3, ITGB1 mRNAs in AD cells and of ITGB1, TLN1 and VCL mRNAs in MDA-MB-231 MCS. RPM-exposed MCF-7 cells revealed a down-regulation in the gene expression of FAK1, PXN, TLN1, VCL and CDH1 in AD cells and PXN, TLN and CDH1 in MCS. An interaction analysis of the examined genes involved in 3D growth and adhesion indicated a central role of fibronectin, vinculin, and E-cadherin. Live cell imaging of eGFP-vinculin in MCF-7 cells confirmed these findings. β-catenin-transfected MCF-7 cells revealed a nuclear expression in 1g and RPM-AD cells. The target genes BCL9, MYC and JUN of the Wnt/β-catenin signaling pathway were differentially expressed in RPM-exposed MCF-7 cells. These findings suggest that vinculin and β-catenin are key mediators of BCC to form MCS during 24 h of RPM-exposure.
Neurofeedback Learning Is Skill Acquisition but Does Not Guarantee Treatment Benefit: Continuous-Time Analysis of Learning-Curves From a Clinical Trial for ADHD

Neurofeedback for attention deficit/hyperactivity disorder (ADHD) has long been studied as an alternative to medication, promising non-invasive treatment with minimal side-effects and sustained outcome. However, debate continues over the efficacy of neurofeedback, partly because existing evidence for efficacy is mixed and often non-specific, with unclear relationships between prognostic variables, patient performance when learning to self-regulate, and treatment outcomes. We report an extensive analysis on the understudied area of neurofeedback learning. Our data comes from a randomised controlled clinical trial in adults with ADHD (registered trial ISRCTN13915109; N = 23; 13:10 female:male; age 25–57). Patients were treated with either theta-beta ratio or sensorimotor-rhythm regimes for 40 one-hour sessions. We classify 11 learners vs 12 non-learners by the significance of random slopes in a linear mixed growth-curve model. We then analyse the predictors, outcomes, and processes of learners vs non-learners, using these groups as mutual controls. Significant predictive relationships were found in anxiety disorder (GAD), dissociative experience (DES), and behavioural inhibition (BIS) scores obtained during screening. Low DES, but high GAD and BIS, predicted positive learning. Patterns of behavioural outcomes from Test Of Variables of Attention, and symptoms from adult ADHD Self-Report Scale, suggested that learning itself is not required for positive outcomes. Finally, the learning process was analysed using structural-equations modelling with continuous-time data, estimating the short-term and sustained impact of each session on learning. A key finding is that our results support the conceptualisation of neurofeedback learning as skill acquisition, and not merely operant conditioning as originally proposed in the literature.
Multitrait genetic association analysis identifies 50 new risk loci for gastro-oesophageal reflux, seven new loci for Barrett’s oesophagus and provides insights into clinical heterogeneity in reflux diagnosis

Objective Gastro-oesophageal reflux disease (GERD) has heterogeneous aetiology primarily attributable to its symptom-based definitions. GERD genome-wide association studies (GWASs) have shown strong genetic overlaps with established risk factors such as obesity and depression. We hypothesised that the shared genetic architecture between GERD and these risk factors can be leveraged to (1) identify new GERD and Barrett’s oesophagus (BE) risk loci and (2) explore potentially heterogeneous pathways leading to GERD and oesophageal complications. Design We applied multitrait GWAS models combining GERD (78 707 cases; 288 734 controls) and genetically correlated traits including education attainment, depression and body mass index. We also used multitrait analysis to identify BE risk loci. Top hits were replicated in 23andMe (462 753 GERD cases, 24 099 BE cases, 1 484 025 controls). We additionally dissected the GERD loci into obesity-driven and depression-driven subgroups. These subgroups were investigated to determine how they relate to tissue-specific gene expression and to risk of serious oesophageal disease (BE and/or oesophageal adenocarcinoma, EA). Results We identified 88 loci associated with GERD, with 59 replicating in 23andMe after multiple testing corrections. Our BE analysis identified seven novel loci. Additionally we showed that only the obesity-driven GERD loci (but not the depression-driven loci) were associated with genes enriched in oesophageal tissues and successfully predicted BE/EA. Conclusion Our multitrait model identified many novel risk loci for GERD and BE. We present strong evidence for a genetic underpinning of disease heterogeneity in GERD and show that GERD loci associated with depressive symptoms are not strong predictors of BE/EA relative to obesity-driven GERD loci.
Mesoporous TiO_2 Spheres Prepared by an Acid Catalyzed Hydrolysis Method Using Nanocrystalline Cellulose as Template

Mesoporous nanosize TiO2 spheres(SP-TiO2) were prepared using natural polymer nanocrystalline cellulose(NCC) as template.Scanning electron microscopy,transmission electron microscopy,X-ray diffraction,ultraviolet-visible diffuse reflection spectroscopy,and N2 adsorption-desorption were used for the characterization of the SP-TiO2 photocatalyst.The results showed that SP-TiO2 was uniform in size with a diameter of 100-200 nm.SP-TiO2 was composed of smaller crystal particles(10-20 nm). The average mesoporous pore diameter of SP-TiO2 was 8.2-13.5 nm,which increased with increasing calcination temperature.A nano-scale reactor that was formed by bonding between the hydroxyl groups of NCC long-chain can inhibit the growth and aggregation of the TiO2 precursor,promote its self-assembly into spherical structure,and inhibit the phase transformation from anatase to rutile.SP-TiO2 prepared at 600 oC exhibited the highest activity with the phenol degradation percentage of 89%.
Single-phase convective heat transfer in microchannels

A comprehensive review is conducted on the investigations of the forced single-phase convective heat transfer in non-circular microchannels. The observations and results available in the open literature are inspected and compared for better understanding of the physical nature of the heat transfer performance and providing some lines of future research. There seems to be no unequivocal agreement in the understanding on the relative phenomena and the determination of the heat transfer coefficients in microchannels. The study on the interfacial phenomena and interaction at the interface will be the frontier in this area. Appropriate data reduction and the correlating parameters will be the cornerstone of comparability and evaluation for comprehensive investigations. The selection of correlating parameters will actually be the basis for the better understanding and description of new phenomena.
Anticancer Activity of 2-O-caffeoyl Alphitolic Acid Extracted from the Lichen, Usnea barbata 2017-KL-10

Colorectal cancer is one of the life-threatening ailments causing high mortality and morbidity worldwide. Despite the innovation in medical genetics, the prognosis for metastatic colorectal cancer in patients remains unsatisfactory. Recently, lichens have attracted the attention of researchers in the search for targets to fight against cancer. Lichens are considered mines of thousands of metabolites. Researchers have reported that lichen-derived metabolites demonstrated biological effects, such as anticancer, antiviral, anti-inflammatory, antibacterial, analgesic, antipyretic, antiproliferative, and cytotoxic, on various cell lines. However, the exploration of the biological activities of lichens’ metabolites is limited. Thus, the main objective of our study was to evaluate the anticancer effect of secondary metabolites isolated from lichen (Usnea barbata 2017-KL-10) on the human colorectal cancer cell line HCT116. In this study, 2OCAA exhibited concentration-dependent anticancer activities by suppressing antiapoptotic genes, such as MCL-1, and inducing apoptotic genes, such as BAX, TP53, and CDKN1A(p21). Moreover, 2OCAA inhibited the migration and invasion of colorectal cancer cells in a concentration-dependent manner. Taken together, these data suggest that 2OCAA is a better therapeutic candidate for colorectal cancer.
Crystal structure of 3-phenylquinoxalin-2(1H)-one, C14H10N2O

Abstract C14H10N2O, orthorhombic, Pna21 (no. 33), a = 17.6745(7) Å, b = 5.3858(2) Å, c = 22.4721(9) Å, V = 2139.1 Å3, Z = 8, Rgt(F) = 0.0386, wRref(F2) = 0.1092, T = 200 K.
An Assessment of the Energy Poverty and Gender Nexus towards Clean Energy Adoption in Rural South Africa

South Africa has about 2.5 million households without electricity access, most of which are located in rural areas and urban informal settlements. The nexus of energy poverty and gender is at play in the affected communities, as women and girls are culturally stereotyped with the task of collecting unclean fuels (e.g., firewood) and using these for their households’ energy demands. Therefore, this study prioritized rural women and girls as respondents in the provinces most affected by gendered energy poverty (GEP) in the country. The study was carried out in selected rural unelectrified areas of Limpopo, Mpumalanga, and KwaZulu-Natal provinces using structured interviews. The study revealed that GEP in the rural areas has exposed women and girls living there to security concerns, health hazards, premature death, domestic fire accidents, time poverty, income poverty, illiteracy, drudgery in households and farm tasks, etc., at different levels of severity. It also showed the effects of perceptions, age, income, and culture on the choice of energy use among the respondents. Mitigation strategies against GEP in rural South African communities through clean energy adoption are also proposed in this paper.
Running title: THE BRAIN ENDOCAST OF Riograndia riograndensis Academy Section: EARTH SCIENCES

Recently, the morphology and encephalization of the brain endocast of the Triassic non-mammaliaform probainognathian cynodont Riograndia guaibensis were studied. Here, we analyzed the brain endocast of an additional specimen of this species. The new endocast shows well-defi ned olfactory bulbs and a median sulcus dividing the hemispheres, traits that were not clearly observed in the fi rst studied specimen. Encephalization quotients were also calculated, revealing similar values to other nonmammaliaform cynodonts and lower than those of the fi rst analyzed specimen. The analyzed cranium is slightly larger than the fi rst studied one and may represent an advanced ontogenetic stage. Hence, these differences may be related to the intraspecifi c variation of this cynodont or alternatively, to the preservation of each specimen.
DNA Methylation in Genetic and Sporadic Forms of Neurodegeneration: Lessons from Alzheimer’s, Related Tauopathies and Genetic Tauopathies

Genetic and sporadic forms of tauopathies, the most prevalent of which is Alzheimer’s Disease, are a scourge of the aging society, and in the case of genetic forms, can also affect children and young adults. All tauopathies share ectopic expression, mislocalization, or aggregation of the microtubule associated protein TAU, encoded by the MAPT gene. As TAU is a neuronal protein widely expressed in the CNS, the overwhelming majority of tauopathies are neurological disorders. They are characterized by cognitive dysfunction often leading to dementia, and are frequently accompanied by movement abnormalities such as parkinsonism. Tauopathies can lead to severe neurological deficits and premature death. For some tauopathies there is a clear genetic cause and/or an epigenetic contribution. However, for several others the disease etiology is unclear, with few tauopathies being environmentally triggered. Here, we review current knowledge of tauopathies listing known genetic and important sporadic forms of these disease. Further, we discuss how DNA methylation as a major epigenetic mechanism emerges to be involved in the disease pathophysiology of Alzheimer’s, and related genetic and non-genetic tauopathies. Finally, we debate the application of epigenetic signatures in peripheral blood samples as diagnostic tools and usages of epigenetic therapy strategies for these diseases.
Does God Condone Assault? How Religious Commitment Impacts Trauma Following Sexual Abuse

Sexual assault has been defined as crimes that include attacks or attempted attacks generally involving unwanted sexual contact between victim and offender. After experiencing assault, survivors look for support and belief often turning to their religious communities. It has been found through prior research that the confession of assault has been received on an inconsistent reaction basis. Prior research has also found that religion is often used as a protective shield for perpetrators to hide behind and a roadblock for survivors. Prior research has also primarily focused on investigating reactions to assault in religious communities for either male female victims with very little research done to compare the two. The purpose of this study is to assess how positively or negatively religion impacts trauma following sexual assault for both men and women. College students were recruited to complete a series of self-report measures that includes measures of sexual experiences, religiosity and spirituality, PTSD, interpersonal support, and symptoms of anxiety, depression, and stress. Results were used to assess how their religious involvement impacts their trauma. Results of the study showed no significant effect of gender and rape status on religious involvement/spiritual involvement. Results also showed a significant effect of gender and rape status on PTSD. Thesis Mentor: ___________________________________ Dr. Brandon Weiss Honors Director: ___________________________________ Dr. Steven Engel April 2021 Department of Psychology Honors College Georgia Southern University DOES GOD CONDONE ASSAULT 2 To the man who gave me life, for inspiring me to change the world so there are less people like you in it.
Factors Related to Nurse’s Compliance in Administering Intravenous Injection

Background: Nursing care including administer intravenous injection must refer to the standard determined by the hospital. It is preventing medication error that could threaten patient's safety. This study aimed to identify factors related to nurse’s compliance in administering intravenous injection according to the standard operational procedures (SOP). Methods: This study was conducted in October 2018 at one of private hospital, Jakarta Utara. Research methods used a quantitative study with a crosssectional design was completed by 67 respondents. There were 67 respondents obtained by purposive sampling technique, with questionnaire and observation methods. Results: Univariate analysis that majority respondents were early adulthood 83.6%, female 89.6%, nurse’s functional of PK II 35.8%, and bachelor education level 79.1%, having good knowledge 88.1%, negative attitude 50.7%, high motivation 65.7%, compliance to SOP 61.2%. Bivariate statistical analysis found that attitudes and motivation were significantly related to nurses’ compliance in administering intravenous injection with p value < 0, 05. Conclusion: Nursing care must refer to SOP from hospital to increase patient safety and improve quality of health care.
No Evidence of Reduced Contrast Sensitivity in Migraine-with-Aura for Large, Narrowband, Centrally Presented Noise-Masked Stimuli

Individuals with migraine aura show differences in visual perception compared to control groups. Measures of contrast sensitivity have suggested that people with migraine aura are less able to exclude external visual noise, and that this relates to higher variability in neural processing. The current study compared contrast sensitivity in migraine with aura and control groups for narrow-band grating stimuli at 2 and 8 cycles/degree, masked by Gaussian white noise. We predicted that contrast sensitivity would be lower in the migraine with aura group at high noise levels. Contrast sensitivity was higher for the low spatial frequency stimuli, and decreased with the strength of the masking noise. We did not, however, find any evidence of reduced contrast sensitivity associated with migraine with aura. We propose alternative methods as a more targeted assessment of the role of neural noise and excitability as contributing factors to migraine aura.
Analysis of Translation of Audiovisual Humor in Iranian Series

In recent years, audiovisual translation (AVT) has become omnipresent in different parts of the world; however, dubbing humor restrictions may hamper the translation in that maintaining the humor in the target language (TL) requires a high level of knowledge. Although several studies have attempted to describe the distribution of translation strategies, few systematic attempts are made to analyze the employed AVT strategies in humor series. The present study analyzed the Persian humor expressions’ appropriacy and their translated versions in English through a qualitative study. To this aim, the dominant strategies used to render humor in the translated versions of 3 dubbed Iranian series (i.e., Shamsolemareh, Chimney, and Medical Building) were analyzed. Furthermore, the typologies of the employed audiovisual humor in the series were analyzed. Data analysis illustrated that clumsiness and parody were the most frequent humor typologies used in the series. The 3 strategies of omission, word-for-word translation, and free translation were the most frequent ones. In conclusion, the results indicated the failure to accommodate the Iranian series’ humorous level, preventing the target audience from appreciating humor. Findings can contribute to the improvement of the quality of humor translation.