Miracle-dz/UniFAR-FTUs huggingface dataset:
Mediation analysis of circadian preferences and other behavioral and sociodemographic factors associated with subjective well-being in undergraduate students

ABSTRACT Undergraduate students are usually subjected to a routine with constant pressure, stress, circadian misalignment, and sleep irregularity that impairs their subjective well-being. Recent evidence suggests that circadian preference is also a risk factor for impaired mental health and factors related to subjective well-being. This study aimed to identify the sociodemographic factors associated with subjective well-being and describe the mediating behavioral variables. Between September 2018 and March 2021, 615 Brazilian students enrolled in higher educational institutions completed an electronic form containing questionnaires on subjective well-being, sociodemographic, and behavioral-related factors (convenience sample). A statistical mediation model was applied to describe how these variables influence subjective well-being. We observed that Morningness (p < .001), identification with the male gender (p = .010), not working while studying (p = .048), and the practice of Pilates/yoga (p = .028) were associated with greater subjective well-being. Except for employment status, no direct effects were observed, which reinforces the need to consider a multidimensional approach. The relationship between subjective well-being and sociodemographic factors exists only in the presence of behavioral mediators, specifically perceived stress, daytime sleepiness, symptoms of depression, sleep quality, and positive and negative affects. Future work should investigate in more detail the impact of sleep, stress, and circadian preferences on this relationship. GRAPHICAL ABSTRACT
Salmonella effector SopF regulates PANoptosis of intestinal epithelial cells to aggravate systemic infection

ABSTRACT SopF, a newly discovered effector secreted by Salmonella pathogenicity island-1 type III secretion system (T3SS1), was reported to target phosphoinositide on host cell membrane and aggravate systemic infection, while its functional relevance and underlying mechanisms have yet to be elucidated. PANoptosis (pyroptosis, apoptosis, and necroptosis) of intestinal epithelial cells (IECs) has been characterized as a pivotal host defense to limit the dissemination of foodborne pathogens, whereas the effect of SopF on IECs PANoptosis induced by Salmonella is rather limited. Here, we show that SopF can attenuate intestinal inflammation and suppress IECs expulsion to promote bacterial dissemination in mice infected with Salmonella enterica serovar Typhimurium (S. Typhimurium). We revealed that SopF could activate phosphoinositide-dependent protein kinase-1 (PDK1) to phosphorylate p90 ribosomal S6 kinase (RSK) which down-regulated Caspase-8 activation. Caspase-8 inactivated by SopF resulted in inhibition of pyroptosis and apoptosis, but promotion of necroptosis. The administration of both AR-12 (PDK1 inhibitor) and BI-D1870 (RSK inhibitor) potentially overcame Caspase-8 blockade and subverted PANoptosis challenged by SopF. Collectively, these findings demonstrate that this virulence strategy elicited by SopF aggregates systemic infection via modulating IEC PANoptosis through PDK1-RSK signaling, which throws light on novel functions of bacterial effectors, as well as a mechanism employed by pathogens to counteract host immune defense.
Development and validation of a predictive nomogram for sarcopenia among older people in China

To the Editor: Sarcopenia is defined as age-related loss of gout, or amputation were excluded from this research. skeletalmusclemass and strength,with an incidenceof 5%to 13% inpersons aged>60years. Sarcopenia is considered a major contributor of frailty in the elderly, and frailty is reported tobeassociatedwithpooroutcomesofolderpeople. Sarcopenia can also increase the risk of falls and fractures, leading to lower quality of life, other related diseases, and even death. Thus, early screening of sarcopenia is recommended for older people. The universally accepted diagnostic criteria of sarcopenia include the measurement of muscle mass, muscle strength, and muscle function. Among the muscle mass measurement methods, magnetic resonance imaging, computed tomography, and dual-energy X-ray absorptiometry were difficult to popularize because of the high cost or radiation exposure. Bioelectrical impedance analysis (BIA) is a non-invasive, portable, and economical method;however, its results canbeaffectedbyvarious factors such as hydration status, food intake, and fat distribution. The above deficiencies limit its use in the screening of sarcopenia; thus, a multi-parameter screening tool based on easy-to-get and reliable clinical factors is urgently needed.
Effectiveness of Bedaquiline Use beyond Six Months in Patients with Multidrug-Resistant Tuberculosis

Rationale Current recommendations for the treatment of rifampicin- and multidrug-resistant tuberculosis include bedaquiline (BDQ) used for 6 months or longer. Evidence is needed to inform the optimal duration of BDQ. Objectives We emulated a target trial to estimate the effect of three BDQ duration treatment strategies (6, 7–11, and ⩾12 mo) on the probability of successful treatment among patients receiving a longer individualized regimen for multidrug-resistant tuberculosis. Methods To estimate the probability of successful treatment, we implemented a three-step approach comprising cloning, censoring, and inverse probability weighting. Measurements and Main Results The 1,468 eligible individuals received a median of 4 (interquartile range, 4–5) likely effective drugs. In 87.1% and 77.7% of participants, this included linezolid and clofazimine, respectively. The adjusted probability of successful treatment was 0.85 (95% confidence interval [CI], 0.81–0.88) for 6 months of BDQ, 0.77 (95% CI, 0.73–0.81) for 7–11 months, and 0.86 (95% CI, 0.83–0.88) for ⩾12 months. Compared with 6 months of BDQ, the ratio of treatment success was 0.91 (95% CI, 0.85–0.96) for 7–11 months and 1.01 (95% CI, 0.96–1.06) for ⩾12 months. Naive analyses that did not account for bias revealed a higher probability of successful treatment with ⩾12 months (ratio, 1.09 [95% CI, 1.05–1.14]). Conclusions BDQ use beyond 6 months did not increase the probability of successful treatment among patients receiving longer regimens that commonly included new and repurposed drugs. When not properly accounted for, immortal person–time bias can influence estimates of the effects of treatment duration. Future analyses should explore the effect of treatment duration of BDQ and other drugs in subgroups with advanced disease and/or receiving less potent regimens.
A static paradigm based on illusion-induced VEP for brain-computer interfaces

Objective. Visual evoked potentials (VEPs) have been commonly applied in brain-computer interfaces (BCIs) due to their satisfactory classification performance recently. However, most existing methods with flickering or oscillating stimuli will induce visual fatigue under long-term training, thus restricting the implementation of VEP-based BCIs. To address this issue, a novel paradigm adopting static motion illusion based on illusion-induced visual evoked potential is proposed for BCIs to enhance visual experience and practicality. Approach. This study explored the responses to baseline and illusion tasks including the rotating-tilted-lines illusion and rotating-snakes illusion. The distinguishable features were examined between different illusions by analyzing the event-related potentials and amplitude modulation of evoked oscillatory responses. Main results. The illusion stimuli elicited VEPs in an early time window encompassing a negative component (N1) from 110 to 200 ms and a positive component (P2) between 210 and 300 ms. Based on the feature analysis, a filter bank was designed to extract discriminative signals. The task-related component analysis was used to evaluate the binary classification task performance of the proposed method. Then the highest accuracy of 86.67% was achieved with a data length of 0.6 s. Significance. The results of this study demonstrate that the static motion illusion paradigm has the feasibility of implementation and is promising for VEP-based BCI applications.
Emotional Intelligence and Teachers’ Work Engagement: The Mediating and Moderating Role of Perceived Stress

Abstract A growing number of studies have tested the relationship between personal resources (e.g. emotional intelligence) and indicators of occupational well-being, including work engagement. However, few have examined health-related factors moderating or mediating the pathway from emotional intelligence to work engagement. A better knowledge of this area would contribute substantially to the design of effective intervention strategies. The present study’s main goal was to test the mediating and moderating role of perceived stress in the association between emotional intelligence and work engagement. The participants comprised 1166 Spanish teaching professionals (744 of whom were female and 537 worked as secondary teachers; Mage = 44.28 years). The results showed that perceived stress partially mediated the link between emotional intelligence and work engagement. Moreover, the positive relationship between emotional intelligence and work engagement was strengthened among individuals who scored high in perceived stress. The results suggest that multifaceted interventions targeting stress management and emotional intelligence development may facilitate engagement in emotionally demanding occupations such as teaching.
A nudging scheme to assimilate satellite brightness temperature in a meteorological model: Impact on representation of African mesoscale convective systems

The study presents the implementation of a nudging procedure in a mesoscale meteorological model, and its evaluation in improving the representation of mesoscale convective systems in the region of West Africa. The generation and development of convection are related to processes with different spatial scales, making it often difficult for meteorological models to reproduce their dynamics.
How Work Pattern Transformation May Affect the Mental Health of Older Workers Faced With Unmet Health Care Needs During the Pandemic? A Comparative Investigation of Different Work Models

The mental health of older people fully or partially involved in on-site work should be focused, especially when they are faced with overwhelming strain on the health system during the pandemic. Purpose This study compares the relation between unmet health care needs and mental health of older people with different work patterns. Methods This study uses data of Survey of Health, Aging and Retirement in Europe Corona survey (n = 51,632 to 51,731). Results The unmet health care need results in depression/sadness during the pandemic (0.304, P < 0.01). Besides, such problem is more salient in workers than the nonemployed population (0.066, P < 0.01 for workers; 0.058, P < 0.01 for the nonemployed) and more outstanding in those working on site and with hybrid work model compared with the telecommuters (0.264, P < 0.01 for telecommuters; 0.378, P < 0.01 for on-site workers; 0.437, P < 0.01 for hybrid work model). Conclusions Policymakers should focus on mental health of older people especially for those fully or partially involved in on-site work, when common health care need can be crowded out for limited health care resources during the pandemic.
Honokiol attenuates mitochondrial fission and cell apoptosis by activating Sirt3 in intracerebral hemorrhage

Abstract Background: Sirtuin-3 (Sirt3) has been documented to protect against mitochondrial dysfunction and apoptosis. Honokiol (HKL) is a Sirt3 pharmacological activator with reported neuroprotective effects in multiple neurological disorders. The present study aimed to explore the neuroprotective effects of HKL and the role of Sirt3 following intracerebral hemorrhage (ICH). Methods: An in vivo ICH model in rats was established by injecting autologous blood into the right basal ganglia. PC12 cells were stimulated with hemin. For the in vivo investigation, the modified Neurological Severity Scores and the Morris water maze test were performed to assess neurological deficits. Hematoxylin–Eosin and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining were employed to evaluate the histopathology and apoptosis. Immunohistochemical staining was used to investigate the expression of Sirt3. Adenosine triphosphate (ATP) levels were quantified to assess mitochondrial dysfunction. Cell counting kit-8, lactate dehydrogenase assay, and flow cytometry were used to analyze cell vitality and apoptosis in vitro. Immunofluorescence staining was performed to observe mitochondrial morphology and dynamin-related protein 1 (Drp1) localization to mitochondria. Western blot was applied to quantify the expression of Sirt3, Bax, Bcl-2, cleaved-caspase-3, Drp1, phosphorylation of Drp1 at serine-616, and phosphorylation of Drp1 at serine-637 in vivo and in vitro. Results: HKL treatment alleviated neurological deficits, attenuated the histopathological damage and cell apoptosis, and restored the decreased ATP levels in ICH rats. HKL improved cell survival rate, reduced cell apoptosis, and inhibited mitochondrial fission in PC12 cells. Moreover, both in vivo and in vitro models showed increased phosphorylation of Drp1 at Ser616, and reduced phosphorylation of Drp1 at Ser637. Meanwhile, immunofluorescence co-localization analysis revealed that hemin increased the overlap of Drp1 and mitochondria in PC12 cells. The phosphorylation and mitochondrial translocation of Drp1 were effectively reversed by HKL treatment. Importantly, the selective Sirt3 inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine suppressed these effects. Conclusion: Our findings demonstrated that HKL ameliorated ICH-induced apoptosis and mitochondrial fission by Sirt3, suggesting that HKL has immense prospects for the treatment of ICH.
Value-modulated attentional capture is augmented by win-related sensory cues

Attentional prioritisation of stimuli in the environment plays an important role in overt choice. Previous research shows that prioritisation is influenced by the magnitude of paired rewards, in that stimuli signalling high-value rewards are more likely to capture attention than stimuli signalling low-value rewards; and this attentional bias has been proposed to play a role in addictive and compulsive behaviours. A separate line of research has shown that win-related sensory cues can bias overt choices. However, the role that these cues play in attentional selection is yet to be investigated. Participants in this study completed a visual search task in which they responded to a target shape in order to earn reward. The colour of a distractor signalled the magnitude of reward and type of feedback on each trial. Participants were slower to respond to the target when the distractor signalled high reward compared to when the distractor signalled low reward, suggesting that the high-reward distractors had increased attentional priority. Critically, the magnitude of this reward-related attentional bias was further increased for a high-reward distractor with post-trial feedback accompanied by win-related sensory cues. Participants also demonstrated an overt choice preference for the distractor that was associated with win-related sensory cues. These findings demonstrate that stimuli paired with win-related sensory cues are prioritised by the attention system over stimuli with equivalent physical salience and learned value. This attentional prioritisation may have downstream implications for overt choices, especially in gambling contexts where win-related sensory cues are common.
Evidence of structural rearrangements in ESBL-positive pESI(like) megaplasmids of S.Infantis

Abstract The increasing prevalence of pESI(like)-positive, multidrug-resistant (MDR) S. Infantis in Europe is a cause of major concern. As previously demonstrated, the pESI(like) megaplasmid is not only a carrier of antimicrobial resistant (AMR) genes (at least tet, dfr, and sul genes), but also harbours several virulence and fitness genes, and toxin/antitoxin systems that enhance its persistence in the S. Infantis host. In this study, five prototype pESI(like) plasmids, of either CTX-M-1 or CTX-M-65 ESBL-producing strains, were long-read sequenced using Oxford Nanopore Technology (ONT), and their complete sequences were resolved. Comparison of the structure and gene content of the five sequenced plasmids, and further comparison with previously published pESI(like) sequences, indicated that although the sequence of such pESI(like) ‘mosaic’ plasmids remains almost identical, their structures appear different and composed of regions inserted or transposed after different events. The results obtained in this study are essential to better understand the plasticity and the evolution of the pESI(like) megaplasmid, and therefore to better address risk management options and policy decisions to fight against AMR and MDR in Salmonella and other food-borne pathogens. Graphical representation of the pESI-like plasmid complete sequence (ID 12037823/11). Block colours indicate the function of the genes: red: repB gene; pink: class I integrons (IntI); yellow; mobile elements; blue: resistance genes; green: toxin/anti-toxin systems; grey: mer operon; light green: genes involve in conjugation.
Deficiency of the minor spliceosome component U4atac snRNA secondarily results in ciliary defects in human and zebrafish

Significance Introns must be removed from precursor messenger RNAs for proper eukaryotic gene expression. All major eukaryotic taxa have two types of introns, the major (>99%) and minor (<1%) introns, and two different ribonucleoprotein complexes to excise them, the major and minor spliceosomes. Biallelic mutations of RNU4ATAC, transcribed into the minor spliceosome component U4atac snRNA, were previously identified in patients presenting with three rare genetic syndromes combining variable intensity of microcephaly, growth restriction, bone anomalies, and immunodeficiency. Here, we shed light on a physiopathological mechanism underlying some RNU4ATAC-associated developmental anomalies by reporting biallelic RNU4ATAC mutations in five patients suspected of Joubert syndrome, a well-known ciliopathy. This finding is sustained by cellular and zebrafish models, thus linking minor splicing and primary cilium.
Racial Differences in C-Reactive Protein, Depression Symptoms, and Social Relationships in Older Adults: A Moderated Network Analysis

Introduction We introduce moderated network analysis as an integrative approach to assess the moderation effects of race on the relationship between C-reactive protein (CRP) and depression symptoms in older adults. This study further explores how the observed relationships differ adjusting for social relationships. Methods This secondary analysis of cross-sectional data from the National Social Life, Health, and Aging Project (2010–2011) includes 2,880 older adults. We used different depression symptom domains (depressed affect, low positive affect, somatic symptoms, and interpersonal problems) from the Center for Epidemiologic Studies-Depression Scale. Social relationships were assessed with measures of social integration, social support, and social strain. The moderated networks were constructed using the R-package mgm. The racial moderator was coded as White/African American racial groups. Results In the moderated networks of CRP and depression symptoms, CRP–“interpersonal problems” edge was present only among African Americans. CRP–“somatic symptoms” edge was present in both racial groups with equal edge weights. After adjusting for social relationships, the aforementioned patterns remained the same, but the edge weights were attenuated. We additionally observed CRP–social strain and social integration–“depressed affect” edges only in African Americans. Discussion Race may moderate the relationship between the CRP and depression symptoms in older adults and social relationships might be important covariates to consider while analyzing them. This study as an initiation point; future network investigations would benefit from leveraging more contemporary cohorts of older adults, gaining a large sample size with diverse racial/ethnic backgrounds, and important covariates. Several important methodological issues of the current study are addressed.
Goodness-of-fit test based on correcting moments of modified entropy estimator

In this paper, we first propose a new estimator of entropy for continuous random variables. Our estimator is obtained by correcting the coefficients of Vasicek's [A test for normality based on sample entropy, J. R. Statist. Soc. Ser. B 38 (1976), pp. 54–59] entropy estimator. We prove the consistency of our estimator. Monte Carlo studies show that our estimator is better than the entropy estimators proposed by Vasicek, Ebrahimi et al. [Two measures of sample entropy, Stat. Probab. Lett. 20 (1994), pp. 225–234] and Correa [A new estimator of entropy, Commun. Stat. Theory Methods 24 (1995), pp. 2439–2449] in terms of root mean square error. We then derive the non-parametric distribution function corresponding to our proposed entropy estimator as a piece-wise uniform distribution. We also introduce goodness-of-fit tests for testing exponentiality and normality based on the said distribution and compare its performance with their leading competitors.
Comment on “Arrival time in quantum mechanics” and “Time of arrival in quantum mechanics”

Contrary to claims contained in papers by Grot, Rovelli, and Tate [Phys. Rev. A 54, 4676 1996)] and Delgado and Muga [Phys. Rev. A 56, 3425 (1997)], the ``time operator,'' which I have constructed [Rep. Math. Phys. 6, 361 (1974)] in an axiomatic way, is a self-adjoint operator existing in a usual Hilbert space of (nonrelativistic or relativistic) quantum mechanics.
Graupel and Hail Terminal Velocities: Does a “Supercritical” Reynolds Number Apply?

AbstractThis study characterizes the terminal velocities of heavily rimed ice crystals and aggregates, graupel, and hail using a combination of recent drag coefficient and particle bulk density observations. Based on a nondimensional Reynolds number (Re)–Best number (X) approach that applies to atmospheric temperatures and pressures where these particles develop and fall, the authors develop a relationship that spans a wide range of particle sizes. The Re–X relationship can be used to derive the terminal velocities of rimed particles for many applications. Earlier observations suggest that a “supercritical” Reynolds number is reached where the drag coefficient for large spherical ice—hail—drops precipitously and the terminal velocities increase rapidly. The authors draw on observations and model simulations for slightly roughened large ice particles that suggest that the critical Reynolds number is dampened and that the rapid increase in the terminal velocity of smooth spherical ice particles rarely occur.