Miracle-dz/UniFAR-FTUs huggingface dataset:
Antimicrobial resistance and genotyping of Pseudomonas aeruginosa isolated from the ear canals of dogs in Japan

The strong bond between dogs and their owners creates a close association that could result in the transfer of antibiotic-resistant bacteria from canines to humans, potentially leading to the spread of antimicrobial resistance genes. Pseudomonas aeruginosa, a common causative agent of persistent ear infections in dogs, is often resistant to multiple antibiotics. Assessing the antimicrobial resistance profile and genotype of P. aeruginosa is crucial for the appropriate use of veterinary pharmaceuticals. However, in recent years, few studies have been conducted on this bacterium in Japan. We determined the antimicrobial resistance profile and genotype of P. aeruginosa isolated from the ear canal of dogs in Japan in 2020. Analysis of antimicrobial resistance using disk diffusion tests indicated a high frequency of resistance to most antimicrobial agents. Particularly, 29 isolates from the ear canals of the 29 affected dogs (100%) were resistant to cefovecin, cefpodoxime, and florfenicol; however, they were susceptible to cefepime and piperacillin/tazobactam. Only 3.4, 10.3, and 10.3% of the isolates were resistant to ceftazidime, tobramycin, and gentamicin, respectively. Furthermore, upon analyzing the population structure using multilocus sequence typing, a considerably large clonal complex was not observed in the tested isolates. Three isolates, namely ST3881, ST1646, and ST532, were clonally related to the clinically isolated sequence types in Japan (such as ST1831, ST1413, ST1812, and ST1849), which is indicative of dog-to-human transmission. Considering the variation in antibiotic resistance compared to that reported by previous studies and the potential risk of dog-to-human transmission, we believe that the survey for antimicrobial resistance profile and population structure should be continued regularly. However, the prevalence of multidrug-resistant P. aeruginosa in dogs in Japan is not a crisis.
MOLECULAR SYSTEMATIC STUDY OF TWO SOLANACEOUS GENERA DATURA L. AND BRUGMANSIA PERS. BASED ON ITS SEQUENCES OF NRDNA

Dhanya C. 1 , Shabir A. Rather 2 and Devipriya V 3 . 1. Research & PG Department of Botany, SN College, Kollam, Kerala, South India, 2. Department of Botany, Delhi University, New Delhi, India, 3. Department of Botany, SN College, Chempazhanthy, Kerala, South India. Manuscript Info Abstract . Manuscript History Received: 13 August 2018 Final Accepted: 15 September 2018 Published: October 2018 Phylogenetic analysis was performed based on ITS 1 and 2 sequences to determine monophyly of Datura and Brugmansia and to understand their relationships. The results support the splitting of Datura and Brugmansia into two distinct genera (BS 100 & 91). D. ceratocaula earlier considered as the connecting link between the two genera, is nested within the Datura clade (BS 57) as a distinct species. Although the three conventional sections of Datura viz. Dutra, Ceratocaulis & Datura (Stramonium) stand well supported (BS 100, 100 & 83), D. kymatocarpa, D. leichhardtii and D. pruinosa appear shifted from Section Dutra to Section Datura, corroborating earlier observations. D. discolor remains nested within the Section Dutra, refuting earlier suggestions as intermediary or ancestral role. But the divergence of D. discolor from the remaining taxa within the section Dutra as a clearly supported subclade (BS 100) may be suggestive of the subdivision of Section Dutra into two subsections. This subdivision of section Dutra however remains to be appraised using cladistical studies incorporating both molecular and morphological data. The infrageneric grouping of Brugmansia into cold and warm groups viz. sections Sphaecarpium and Brugmansia exclusively on the basis of fruit shape and habitat lacks adequate support in the present analysis. But fruit shape appears not consistent as the warm climatic inhabitant B. aurea with trumpet shaped flowers sports egg-shaped fruits. Hence we propose subdivision of the genus into two sections on the basis of the more stable corolline form, and the proposition is well supported by molecular data viz. section Brugmansia (with trumpet and/or funnel shaped flowers, with fruits fusiform or egg-shaped) and section Sanguinea (with tubular flowers and consistently egg-shaped or short oval fruits).
OX40L-Armed Oncolytic Virus Boosts T-cell Response and Remodels Tumor Microenvironment for Pancreatic Cancer Treatment

Rationale: The resistance of pancreatic ductal adenocarcinoma (PDAC) to immunotherapies is caused by the immunosuppressive tumor microenvironment (TME) and dense extracellular matrix. Currently, the efficacy of an isolated strategy targeting stromal desmoplasia or immune cells has been met with limited success in the treatment of pancreatic cancer. Oncolytic virus (OV) therapy can remodel the TME and damage tumor cells either by directly killing them or by enhancing the anti-tumor immune response, which holds promise for the treatment of PDAC. This study aimed to investigate the therapeutic effect of OX40L-armed OV on PDAC and to elucidate the underlying mechanisms. Methods: Murine OX40L was inserted into herpes simplex virus-1 (HSV-1) to construct OV-mOX40L. Its expression and function were assessed using reporter cells, cytopathic effect, and immunogenic cell death assays. The efficacy of OV-mOX40L was then evaluated in a KPC syngeneic mouse model. Tumor-infiltrating immune and stromal cells were analyzed using flow cytometry and single-cell RNA sequencing to gain insight into the mechanisms of oncolytic virotherapy. Results: OV-mOX40L treatment delayed tumor growth in KPC tumor-bearing C57BL/6 mice. It also boosted the tumor-infiltrating CD4+ T cell response, mitigated cytotoxic T lymphocyte (CTL) exhaustion, and reduced the number of regulatory T cells. The treatment of OV-mOX40L reprogrammed macrophages and neutrophils to a more pro-inflammatory anti-tumor state. In addition, the number of myofibroblastic cancer-associated fibroblasts (CAF) was reduced after treatment. Based on single-cell sequencing analysis, OV-mOX40L, in combination with anti-IL6 and anti-PD-1, significantly extended the lifespan of PDAC mice. Conclusion: OV-mOX40L converted the immunosuppressive tumor immune microenvironment to a more activated state, remodeled the stromal matrix, and enhanced T cell response. OV-mOX40L significantly prolonged the survival of PDAC mice, either as a monotherapy or in combination with synergistic antibodies. Thus, this study provides a multimodal therapeutic strategy for pancreatic cancer treatment.
THE PROBABLE RELATION BETWEEN TOXOPLASMA GONDII AND DIABETES MELLITUS

Present study aims to determine probable relation between Toxoplasma gondii infection and diabetes mellitus by investigating serum levels of insulin and anti-Toxoplasma antibodies through ELISA among diabetic and non-diabetic persons in Lahore, Pakistan. Results showed that insulin level was higher in subjects positive for Toxoplasma infection as compared to Toxoplasma free one’s (p<0.05). The analysis revealed that Toxoplasma infection was more prevalent in diabetic patients (p<0.05) than non-diabetic individuals. Results also showed that insulin level was higher in persons with chronic infection. Gender-wise comparison revealed that in males, insulin level had significant association with Toxoplasma infection (p<0.05). It was concluded that participants who had Toxoplasma infection had higher insulin level that showed the probable relation of infection with diabetes.
Lignin, the Lignification Process, and Advanced, Lignin-Based Materials

At a time when environmental considerations are increasingly pushing for the application of circular economy concepts in materials science, lignin stands out as an under-used but promising and environmentally benign building block. This review focuses (A) on understanding what we mean with lignin, i.e., where it can be found and how it is produced in plants, devoting particular attention to the identity of lignols (including ferulates that are instrumental for integrating lignin with cell wall polysaccharides) and to the details of their coupling reactions and (B) on providing an overview how lignin can actually be employed as a component of materials in healthcare and energy applications, finally paying specific attention to the use of lignin in the development of organic shape-memory materials.
Gemfibrozil-trans-cinnamic acid co-crystal: Synthesis, characterization, in vitro solubility and cell viability studies

The co-crystallization is a technic used to improve drug properties, mainly for drugs belonging to classes II and IV of the Biopharmaceutical Classification System like gemfibrozil (GEM). This study aims to synthesize a new co-crystal of GEM and trans-cinnamic acid (TCA) through the mechanochemistry method, characterize it using powder X-ray diffraction, Fourier-transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis, and determine the equilibrium solubility and cytotoxicity of the new crystalline form. The characterization techniques indicated that the cocrystal was successfully formed, especially because they revealed a unique X-ray pattern and a single endothermic event, different from the isolated substances. The concomitant use of GEM and TCA was shown to be safe at concentrations up to 100 M in models using Caco-2 cells. The formation of the GEM: TCA co-crystal was successful, and the new co-crystal was not cytotoxic at the concentrations analyzed.
The impact of CSR and corporate ability on purchase intention in e-commerce websites: an examination across genders

ABSTRACT This study aims to investigate the impact of corporate social responsibility (CSR) and corporate ability (CA) on customer purchase intention on e-commerce websites. Also, the effect of trust as a mediator between CSR-purchase intention and CA-purchase intention relationships is examined. Finally, the role of gender as a moderator in the relationships between model variables is explored. This research is a type of Structural Equation Model (SEM), and data were collected from customers of e-commerce websites who made online purchases at least five times a year. The results reveal the positive effect of CA and negative effect of CSR for e-commerce websites in promoting purchase intention of their customers. Additionally, gender moderates the relationship between CSR-purchase intention and CA-purchase intention. The finding can be useful to startup companies that operate in the field of e-commerce. Further, online vendors can improve the quality of their service using the findings of this research.
Turbulence Measurements from Compliant Moorings. Part II: Motion Correction

AbstractAcoustic Doppler velocimeters (ADVs) are a valuable tool for making high-precision measurements of turbulence, and moorings are a convenient and ubiquitous platform for making many kinds of measurements in the ocean. However, because of concerns that mooring motion can contaminate turbulence measurements and that acoustic Doppler profilers make middepth velocity measurements relatively easy, ADVs are not frequently deployed from moorings. This work demonstrates that inertial motion measurements can be used to reduce motion contamination from moored ADV velocity measurements. Three distinct mooring platforms were deployed in a tidal channel with inertial-motion-sensor-equipped ADVs. In each case, motion correction based on the inertial measurements reduces mooring motion contamination of velocity measurements. The spectra from these measurements are consistent with other measurements in tidal channels and have an slope at high frequencies—consistent with Kolmogorov’s theory of isotropic turbulence.
The Role of S100A6 in Human Diseases: Molecular Mechanisms and Therapeutic Potential

S100A6, also known as calcyclin, is a low-molecular-weight Ca2+-binding protein from the S100 family that contains two EF-hands. S100A6 is expressed in a variety of mammalian cells and tissues. It is also expressed in lung, colorectal, pancreatic, and liver cancers, as well as other cancers such as melanoma. S100A6 has many molecular functions related to cell proliferation, the cell cycle, cell differentiation, and the cytoskeleton. It is not only involved in tumor invasion, proliferation, and migration, but also the pathogenesis of other non-neoplastic diseases. In this review, we focus on the molecular mechanisms and potential therapeutic targets of S100A6 in tumors, nervous system diseases, leukemia, endometriosis, cardiovascular disease, osteoarthritis, and other related diseases.
Effect of Team Characteristics, Organizational Justice, and Work Ethic on Teacher Job Performance

The objective of the study was to examine the effect of team characteristics, organizational fairness, and work ethic on the performance of teachers of elementary school in East Jakarta. The research method is quantitative with the survey. The sample of the study was 209 teachers drawn with simple random technics. Data analysis uses descriptive statistics and path analysis. The results conclude: there is a positive direct effect of team characteristics, organizational fairness, and work ethic on teacher performance; there is a positive direct effect of team characteristics and organizational fairness on teacher work ethics; And there is a direct influence of team characteristics directly positive effect on organizational justice. In conclusion, team characteristics, organizational justice, and work ethics have a direct and positive impact on the performance of teachers of Elementary School in East Jakarta. There are suggestions that team characteristics, organizational fairness, and work ethics need to be improved in an effort to improve teacher performance
Comparison of viscous budesonide and fluticasone in the treatment of patients with eosinophilic esophagitis: a systematic review and meta-analysis

Background Steroids are an important pharmacologic treatment in patients with eosinophilic esophagitis (EoE). Fluticasone and budesonide are the 2 main steroid medications used in EOE treatment, but current United States (US) guidelines do not recommend one agent over the other. In this study, we conducted a meta-analysis to compare important patient outcomes when both agents are used. Methods A comprehensive search of MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, and Scopus was performed from each database’s inception to March 29th, 2023. Two independent reviewers systematically identified trials that compared the effect of budesonide vs. fluticasone in the management of EoE. A meta-analysis was performed using a fixed-effects model. The primary outcome was the histologic response (defined as an eosinophil count <15 per high-power field) which reflects the response to treatment. Results Three studies met our inclusion criteria and were included in the analysis, with a total of 272 patients. All studies were carried out in the US and 1 was a randomized controlled trial. Our meta-analysis showed no statistically significant difference with the use of budesonide compared to fluticasone in achieving a histologic response (odds ratio 1.29, 95% confidence interval 0.77-2.14; P=0.34; I2=0%). Conclusion Our systematic review and meta-analysis indicated no difference between budesonide and fluticasone in achieving a histologic response in patients with EoE.
Raw Jackfruit (Artocarpus heterophyllus) Seedmeal: Effect on Growth Performance, Organ Weight, Carcass Yield, and Economic Production of Guinea Fowl Keets

Ensuring food security and sustainable agriculture is vital as the global population expands, leading to increased protein demand. However, competitive demand for feed ingredients such as maize and soybeans has driven up costs. As a result, alternative feed sources, including Jackfruit by-products like seeds, have been explored. This study aimed to assess the effects of incorporating raw jackfruit seedmeal (JSM) into the diet of guinea fowl keets, specifically regarding growth performance, carcass yield, organ weights, and economic production. The seven-week study involved 120 keets divided into different treatments, each replicated three times. Treatments 2, 3, and 4 included JSM at 10%, 15%, and 20%, respectively, while treatment one served as the control without JSM. Increasing the level of JSM resulted in a consistent decline in all relevant indicators, as observed through proximate analysis. Treatment four, which included 20% JSM, exhibited the best performance in terms of final weight (745g) and daily weight gain (15g). Notably, the gizzard was the most significant parameter among carcass features, with treatment four showing the highest value (3.95%), while the control diet had the lowest value (2.83%). Diets containing 15% and 20% JSM had the most favorable outcomes in terms of feed cost per weight gain, with the 20% JSM diet demonstrating the highest gross margin and revenue. Importantly, dietary inclusion of JSM did not significantly impact the performance of guinea fowl keets, and incorporating 20% JSM into their diet resulted in a significant increase in productivity and feed utilization.
RESOURCE USE EFFICIENCY OF WHEAT SEED PRODUCTION: A CASE FROM CHITWAN, NEPAL

Wheat is staple food for more than thirty percent of the world population and is also a rich source of minerals, proteins, vitamins and dietary fibers. Quality seed is the major reason behind the low productivity of wheat in Nepal. The study was design to investigate the cost, return, and resource use efficiency of wheat seed production in Chitwan district of Nepal. The study used 160 randomly sampled sample of wheat seed producer. Cobb-Douglas production function and benefit cost ratio were used to estimate the resource use efficiency during wheat seed production through data collected using semi-structured interview schedule to meet the objective. The average total cost of wheat seed production with subsidy was NRs. 59440 per hectare and average total cost of wheat seed production without subsidy was NRs. 60731 per hectare. The yield in study area was found 2771.02 kg. The return, profit with subsidy and profit without subsidy from wheat seed production was NRs. 69275.49, NRs. 9835.04 and NRs. 8544.46 in the study area respectively. The benefit cost ratio of wheat seed production in the study area with subsidy was found 1.31 and 1.28 without subsidy. The wheat seed production was found increasing return to scale (RTS = 1.106) in the study area. Seed and Machine were underutilized and other inputs like Farmyard Manure (FYM), chemical fertilizer, labor and other cost were over utilized while producing wheat seed in Chitwan district. The study revealed we can increase profit by optimization of input uses.
Think-Pair-Share in the Development of Grade 7 Student Multiply and Divide Decimal Mathematical Achievement

In this study, the effectiveness of a learning management plan using the think-pair-share technique on students' learning of decimal multiplication and division was investigated. The quasi-experimental study involved 42 grade 7 students in a public school in Thailand, who were simple random sampling. The study used a learning management plan, a learning achievement test, and a scoring rubric as instruments and employed the effectiveness index and a paired samples t-test to analyze the results. The results showed that the think-pair-share technique was effective in improving the student’s understanding of decimal multiplication and division and that the students demonstrated learning outcomes on most topics and met the requirements on the post-test. The study also found that there was a significant improvement in the student’s scores on the pre-test and post-test.
Functionalized Akiyama tips for magnetic force microscopy measurements

In this work we have used focused electron beam induced deposition of cobalt to functionalize atomic force microscopy Akiyama tips for application in magnetic force microscopy. The grown tips have a content of ≈90 % Co after exposure to ambient air. The magnetic tips were characterized using energy dispersive x-ray spectroscopy and scanning electron microscopy. In order to investigate the magnetic properties, current loops were prepared by electron beam lithography. Measurements at room temperature as well as 4.2 K were carried out and the coercive field of ≈6.8 mT of the Co tip was estimated by applying several external fields in the opposite direction of the tip magnetization. Magnetic Akiyama tips open new possibilities for wide-range temperature magnetic force microscopy measurements.
Standardization of Indian cultivars of Brassica spp. for characterization of Xanthomonas campestris pv. campestris races causing black rot disease of crucifer crops

Xanthomonas campestris pv. Campestris (Xcc) causing black rot disease of crucifer crops, is a serious worldwide problem resulting in >50% loss under environmental conditions favorable to the pathogen. For the characterization of Xanthomonas campestris pv. campestris races to standardize the Indian cultivars of Brassica spp. total of 184 representatives lines of the family crucifereae from of five economically important Brassica species, i.e. B. carinata, B. juncea, B. napus, B. oleracea, and B. rapa grown in India were inoculated by 75 strains of Xcc, belonging to three races 1, 4 and 6 in 2012-13 and 2013-14. Disease reaction of these cultivars was compared with the standard differential hosts of crucifer for disease incidence and diseases severity. Indian cultivars of B. juncea (Pusa Bold, Pusa Varuna, Pusa Vijay, Pusa Mustard-21, Pusa Mustard-25, Pusa Sag), B. olerecea var. botrytis (Pusa Sukti), B. olerecea var. capitata (KGMR) and B. rapa (Pusa Swarnima) were at par with the standard differentials of respective Brassica species and thus could be suitable to useas alternative differential hosts for characterization of Xcc races. 20 cultivars of Brassica spp. grown in India were further validated as differential crucifer host to characterize the races of Xcc on the molecular basis using four set of primers, viz. CAPS (ACS2) CAPS (BTPT) and CAPS (NDPK3) and among them, Br 019305 primer amplified at 906 bp in all cultivars, while primer CAPS (ACS2), CAPS (BTPT) and CAPS (NDPK3) primers were amplified multiple bands. Combined phylogenetic UPGMA study at 75% similarity coefficient, 19/20 accessions were divided into three groups and distinguished resistant and susceptible cultivars against black rot disease.
Does Learning English Contribute to Ensuring Patient Safety? Egyptian Cardiac Nurses Perceptions

Background: The effect of English is indisputable in the profession of nursing, where English is increasingly becoming the medium of instruction and communication. Therefore, the principal goal of the study was to examine the perceptions of Egyptian nurses in reference to the English language effect on patient safety. Purpose: The study was implemented in a cardiothoracic Centre that provides free-of-charge care for patients with heart diseases. Design and methods: In this pursuit, a mixed-method approach was employed as two data collection instruments were constructed and utilized following the review of literature and based on the patient international safety goals recommended by the Joint Commission International (JCI). The first instrument is a 39-item questionnaire and the second is a semi-structured interview. The number of nurses participated in the study was (n=94); 86 nurses participated in the questionnaire whereas eight expert nurses were recruited for the semi-structured interview. Results: The results revealed that the English language is an essential element in the process of ensuring patient safety for Egyptian nurses and its effect is revealed through the international safety goals. Based on the study, nurses are advised to be proficient in using the English language so as to provide the best possible care for cardiac patients.