Miracle-dz/UniFAR-FTUs huggingface dataset:
The Role of Information Technology Mindfulness in the Postadoption Stage of Using Personal Health Devices: Cross-Sectional Questionnaire Study in Mobile Health

Background Although personal health devices (for example, smartwatches, fitness trackers and intelligent bracelets) offer great potential to monitor personal fitness and health parameters, many users discontinue using them after a few months. Thus, it is critical to study the postadoption behaviors of current users to enhance their engagement with personal health devices and use behaviors. However, there is little empirical research on the factors affecting users’ engagement in beneficial use behaviors. Mindfulness and identity are not new topics, but the applications of these concepts in the field of information systems are emerging themes. Information technology (IT) mindfulness has been conceptualized in previous studies; however, little is known about the antecedents and consequences of IT mindfulness in the mobile health (mHealth) context. Objective The main aim of this study is to explore both IT identity and IT mindfulness to develop a new ground for research in the domain of mHealth postadoption. Thus, we aim to explain why users should be fully mindful of their engagement with PHDs and what could be the consequences and implications. Methods This study proposes that IT mindfulness can play an important role in improving the use behaviors of users. Through a web-based survey with 450 current users of a personal health device, this paper tests the relationship between IT identity and IT mindfulness in the postadoption stage of using personal health devices. Results We found that IT identity significantly shapes IT mindfulness associated with PHDs. Moreover, the IT identity–IT mindfulness relationship is negatively moderated by individuals’ perceived health status (P=.003). Finally, the results of this study show that IT mindfulness can significantly predict automatic use behaviors (eg, continued intention to use), active use behaviors (eg, feature use and enhanced use behaviors), and commitment behaviors in using personal health devices (eg, positive word-of-mouth intention). Conclusions The findings of this study provide implications for both research and practice. This study can contribute to our current understanding of IT mindfulness by developing and empirically testing a research model that explains the determinants and outcomes of the IT mindfulness construct in the context of personal health devices. The results imply that IT mindfulness significantly helps individuals express their alertness, awareness, openness, and orientation in the present in their postadoption interactions with smart devices used for health care purposes. Finally, our findings may assist practitioners and IT developers in designing mindfulness-supporting PHDs. Owing to the impact of IT mindfulness on postadoption behaviors, its 4 dimensions could be used for developing PHD technologies. Moreover, PHD developers may need to direct their efforts toward increasing IT mindfulness by reinforcing IT identity to serve and retain a wide range of target users.
The HIV-1 latent reservoir is largely sensitive to circulating T cells

HIV-1-specific CD8+ T cells are an important component of HIV-1 curative strategies. Viral variants in the HIV-1 reservoir may limit the capacity of T cells to detect and clear virus-infected cells. We investigated the patterns of T cell escape variants in the replication-competent reservoir of 25 persons living with HIV-1 (PLWH) durably suppressed on antiretroviral therapy (ART). We identified all reactive T cell epitopes in the HIV-1 proteome for each participant and sequenced HIV-1 outgrowth viruses from resting CD4+ T cells. All non-synonymous mutations in reactive T cell epitopes were tested for their effect on the size of the T cell response, with a≥50% loss defined as an escape mutation. The majority (68%) of T cell epitopes harbored no detectable escape mutations. These findings suggest that circulating T cells in PLWH on ART could contribute to control of rebound and could be targeted for boosting in curative strategies.
Undetectable viral RNA in oocytes from SARS-CoV-2 positive women

Abstract A central concern for the safe provision of ART during the current coronavirus disease 2019 (CODIV-19) pandemic is the possibility of vertical transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection through gametes and preimplantation embryos. Unfortunately, data on SARS-CoV-2 viral presence in oocytes of infected individuals are not available to date. We describe the case of two women who underwent controlled ovarian stimulation and tested positive to SARS-CoV-2 infection by PCR on the day of oocyte collection. The viral RNA for gene N was undetectable in all the oocytes analyzed from the two women.
Effect of different vermicomposts under integrated nutrient management on soil fertility and productivity of rice

Studies on effect of different vermicomposts under integrated nutrient management (INM) on soil fertility status, soil humic substances, grain yield and benefit cost ratio in rice were conducted at Anakapalle during wet season 2009 and 2010. Rice was grown with 12 treatments, consisting of INM practices (where 50% or 75% recommended dose of fertilizer (RDF) was integrated with vermicomposts prepared from sugarcane trash, weeds, vegetable waste and rice straw) and certified organic manures. The data indicated that, there was no change in the pH and EC of the soil, whereas the values of available macro and micro nutrients (N, P, K, Zn, Fe, Cu and Mn) were higher with INM practices, specially when vermi compost prepared from vegetable waste was applied. A distinct decrease was noticed in all the values, when crop was raised without any external supplement of nutrients. Application of vermicomposts enhanced the humic and fulvic acid content in the soil over initial value. Significantly higher grain yield was recorded in 75% RDF + vegetable market waste vermicompost @2.5 t ha−1 and it was on par with 50% Prathista organic manures + 50% chemical fertilizers. The treatment with 100% Prathista organic manures recorded highest (BCR = 2.92).
Differential protein expression in compatible and incompatible pollen-pistil interactions in almond [Prunus dulcis (Miller) D. A. Webb] by 2D-DIGE and HPLC-MS/MS

Summary Self-incompatibility (SI) in almond [Prunus dulcis (Miller) D.A. Webb] is of the gametophytic-type and is controlled by the S-locus, which is highly polymorphic and includes two tightly-linked genes expressed in the pistil (as an SRNase) and in the pollen [as an S haplotype-specific F-box (SFB) protein]. Experimental evidence indicates that other proteins are also needed for the functioning of self-incompatibility. However, to date, S-RNase and SFB are the only components of the SI system identified in almond. Moreover, the cause(s) of a lack of S-RNase activity in selfcompatible almonds remain(s) unclear. This work aimed to identify the other proteins involved in the self- (in)compatible response in almond by comparative quantitative analysis of the differential expression of proteins following incompatible and compatible pollinations. Four self-incompatible almond cultivars were self-pollinated or cross-pollinated, and a homozygous self-compatible almond selection was self-pollinated. Proteins were extracted from the pollinated pistils and analysed by two-dimensional difference gel electrophoresis (2D-DIGE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Searches were performed against the NCBI database using the MASCOT and SEQUEST search engines and Proteome Discoverer v1.4 software. A total of 43 proteins, some with a key role in pollen-pistil interactions, were identified as being differentially expressed following incompatible or compatible pollination. These results provide the first profiles of differential protein expression during self-(in)compatibility in almond.
The effect of deep inspiration breath-hold technique on left anterior descending coronary artery and heart dose in left breast irradiation

Purpose To determine the effect of the deep inspiration breath-hold (DIBH) technique on left anterior descending coronary artery (LAD) region and heart dose in left breast cancer irradiation. Materials and Methods Twenty-five left breast cancer patients who previously received breast-conserving surgery underwent computed tomography (CT) simulation with both free-breathing (FB) and DIBH techniques and four radiation treatment plans. The plan comprised the following with both the FB and DIBH techniques: whole breast (WB), and WB with internal mammary lymph nodes (WB+IMNs). The prescription dose was 50 Gy in 25 fractions. The doses to the LAD region, heart and lungs were compared. Moreover, in-field maximum heart distance (maxHD) and breast volume were analyzed for correlations with the mean heart dose (MHD). Results In the WB plan with DIBH vs. FB techniques, the mean radiation doses to the LAD region, MHD, and the left lung V20 were 11.48 Gy vs. 19.84 Gy (p < 0.0001), 2.95 Gy vs. 5.38 Gy (p < 0.0001), and 19.72% vs. 22.73% (p = 0.0045), respectively. In the WB+IMNs plan, the corresponding values were 23.88 Gy vs. 31.98 Gy (p < 0.0001), 6.43 Gy vs. 10.24 Gy (p < 0.0001), and 29.31% vs. 32.1% (p = 0.0009), respectively. MHD correlated with maxHD (r = 0.925) and breast volume (r = 0.6). Conclusion The use of the DIBH technique in left breast cancer irradiation effectively reduces the radiation doses to the LAD region, heart and lungs. MHD is associated with maxHD and breast size.
Two New Indole Alkaloids from Toad Venom of Bufo bufo gargarizans

Two new indole alkaloids, Bufotenidine B (2) and Bufocarboline A (6), along with seven known indole alkaloids (1, 3–5, and 7–9) and three organic acids (10–12), were isolated from the water extract of toad venom. The structures of the new alkaloids were elucidated by extensive spectroscopic methods. The absolute configurations of 4, 6, and 8 were determined for the first time by electronic circular dichroism (ECD) calculations. The cytotoxic activity of all compounds was tested against human malignant melanoma cells A375 by the MTT method, and no antitumor activity was observed.
The Effects of Long-Term Magnesium Creatine Chelate Supplementation on Repeated Sprint Ability (RAST) in Elite Soccer Players

Aim: The aim of the study was to evaluate the effects of 16 weeks of a low dose of magnesium creatine chelate supplementation on repeated sprint ability test (RAST) results in elite soccer players. Materials: Twenty well-trained soccer players participated in the study. The players were divided randomly into two groups: the supplemented group (SG = 10) and placebo group (PG = 10). Out of the 20 subjects selected for the study, 16 (SG = 8, PG = 8) completed the entire experiment. The SG ingested a single dose of 5500 mg of magnesium creatine chelate (MgCr-C), in 4 capsules per day, which was 0.07 g/kg/d. The PG received an identical 4 capsules containing corn starch. Before and after the study, the RAST was performed. In the RAST, total time (TT), first and sixth 35 m sprint length (s), average power (AP) and max power (MP) were measured. Additionally, before and after the test, lactate LA (mmol/L) and acid–base equilibrium pH (-log(H+)), bicarbonates HCO3− (mmol/L) were evaluated. Also, in serum at rest, creatinine (mg/dL) concentration was measured. Results: After the study, significantly better results in TT, AP and MP were observed in the SG. No significant changes in the RAST results were observed in the PG. After the study, significant changes in the first 35 m sprint, as well as the sixth 35 m sprint results were registered in the SG, while insignificant changes occurred in the PG. A significantly higher creatinine concentration was observed. Also, a higher post-RAST concentration of LA, HCO3− and lower values of pH were observed in April, May and June compared with baseline values. Conclusions: The long timeframe, i.e., 16 weeks, of the low dose of magnesium creatine chelate supplementation improved the RAST results in the SG. Despite the long period of MgCr-C supplementation, in the end of the study, the creatinine level in the SG reached higher but still reference values.
Matrix-Bound Nanovesicles: The Effects of Isolation Method Upon Yield, Purity and Function.

Identification of matrix bound nanovesicles (MBV) as ubiquitous components of the extracellular matrix (ECM) raises questions regarding their biologic functions and their potential theranostic application. Unlike liquid-phase extracellular vesicles (e.g., exosomes), MBV are tightly bound to the ECM which makes their isolation and harvesting more challenging. The indiscriminate use of different methods to harvest MBV can alter or disrupt their structural and/or functional integrity. The objective of the present study was to compare the effect of various MBV harvesting methods upon yield, purity, and biologic activity. Combinations of four methods to solubilize the ECM (collagenase, liberase, or proteinase K, and non-enzymatic elution with potassium chloride) and four isolation methods (ultracentrifugation, ultrafiltration, density barrier, and size exclusion chromatography) were used to isolate MBV from urinary bladder derived ECM. All combinations of solubilization and isolation methods allowed for the harvesting of MBV, however distinct differences were noted. The highest yield, purity, cellular uptake, and biologic activity were seen with MBV isolated by a combination of liberase or collagenase followed by size exclusion chromatography. The combination of proteinase K and ultrafiltration was shown to have detrimental effects on bioactivity. The results show the importance of selecting appropriate MBV harvesting methods for the characterization and evaluation of MBV and for analysis of their potential theranostic application.
Multigenerational and transgenerational effects of environmentally relevant concentrations of endocrine disruptors in an estuarine fish model.

Many pollutants cause endocrine disruption in aquatic organisms. While studies of the direct effects of toxicants on exposed organisms are commonplace, little is known about the potential for toxicant exposures in a parental (F0) generation to affect unexposed F1 or F2 generations (multigenerational and transgenerational effects, respectively), particularly in estuarine fishes. To investigate this possibility, we exposed Inland silversides (Menidia beryllina) to environmentally relevant (low ng/L) concentrations of ethinylestradiol, bifenthrin, trenbolone, and levonorgestrel from 8 hpf to 21 dph. We then measured development, immune response, reproduction, gene expression, and DNA methylation for two subsequent generations following the exposure. Larval exposure to each compound resulted in negative effects in the F0 and F1 generations, and for ethinylestradiol and levonorgestrel, the F2 also. The specific endpoints that were responsive to exposure in each generation varied, but included increased incidence of larval deformities, reduced larval growth and survival, impaired immune function, skewed sex ratios, ovarian atresia, reduced egg production, and altered gene expression. Additionally, exposed fish exhibited differences in DNA methylation in selected genes, across all three generations, indicating epigenetic transfer of effects. These findings suggest that assessments across multiple generations are key to determining the full magnitude of adverse effects from contaminant exposure in early life.
Analysis of Arabidopsis TPK2 and KCO3 reveals structural properties required for K+ channel function

ABSTRACT Arabidopsis thaliana contains five tandem-pore domain potassium channels, TPK1-TPK5 and the related one-pore domain potassium channel, KCO3. Although KCO3 is unlikely to be an active channel, it still has a physiological role in plant cells. TPK2 is most similar to KCO3 and both are localized to the tonoplast. However, their function remains poorly understood. Here, taking advantage of the similarities between TPK2 and KCO3, we evaluated Ca2+ binding to the EF hands in TPK2, and the elements of KCO3 required for K+ channel activity. Presence of both EF-hand motifs in TPK2 resulted in Ca2+ binding, but EF1 or EF2 alone failed to interact with Ca2+. The EF hands were not required for K+ transport activity. EF1 contains two cysteines separated by two amino acids. Replacement of both cysteines with serines in TPK2 increased Ca2+ binding. We generated a two-pore domain chimeric K+ channel by replacing the missing pore region in KCO3 with a pore domain of TPK2. Alternatively, we generated two versions of simple one-pore domain K+ channels by removal of an extra region from KCO3. The chimera and one of the simple one-pore variants were functional channels. This strongly suggests that KCO3 is not a pseudogene and KCO3 retains components required for the formation of a functional K+ channel and oligomerization. Our results contribute to our understanding of the structural properties required for K+ channel activity.
Using genetic evaluation to guide conservation of remnant Juniperus communis (Cupressaceae) populations.

Many critically endangered plant species exist in small, genetically depauperate or inbred populations, making assisted gene flow interventions necessary for long-term population viability. However, before such interventions are implemented, conservation practitioners must consider the genetic and demographic status of extant populations, which are strongly affected by species' life-history traits. In north-western Europe, Juniperus communis, a dioecious, wind-pollinated and bird-dispersed gymnosperm, has been declining for the past century and largely exists in small, isolated and senescent populations. To provide useful recommendations for a recovery plan involving translocation of plants, we investigated genetic diversity and structure of populations in Belgium using four microsatellite and five plastid single-nucleotide polymorphism (SNP) markers. We detected no clonality in the populations, suggesting predominantly sexual reproduction. Populations exhibited high genetic diversity (He = 0.367-0.563) and low to moderate genetic differentiation (FST ≤ 0.133), with no clear geographic structure. Highly positive inbreeding coefficients (FIS = 0.221-0.507) were explained by null alleles, population substructuring, and biparental inbreeding. No isolation by distance was observed among distant populations, but isolation at close geographic proximity was found. Patterns were consistent with high historical gene flow through pollen and seed dispersal at both short and long distances. We also tested four pre-germination treatments among populations to improve germination rates; however, germination rates remained low and only cold-stratification treatments induced germination in some populations. To bolster population regeneration, introductions of cuttings from several source populations are recommended, in combination with in situ management practices that improve seedling survival and with ex situ propagation.
Natriuretic peptides relax human intrarenal arteries through natriuretic peptide receptor type‐A recapitulated by soluble guanylyl cyclase agonists

Natriuretic peptides, BNP and ANP increase renal blood flow in experimental animals. The signalling pathway in human kidney vasculature is unknown. It was hypothesized that BNP and ANP cause endothelium‐independent relaxation of human intrarenal arteries by vascular natriuretic peptide receptor‐A, but not ‐B and ‐C, which is mimicked by agonists of soluble guanylyl cyclase sGC.
The impact of preoperative MRI-based apparent diffusion coefficients on local recurrence and outcome in patients with cerebral metastases

Abstract Background Surgery of single cerebral metastases is standard but frequently fails to achieve local tumour control. Reliable predictors for local tumour progression and overall survival are unknown. MRI-based apparent diffusion coefficients (ADC) correlate with tumour cellularity and invasion. The present study analysed a potential relation between the MRI based apparent diffusion coefficients local recurrence and outcome in patients with brain metastases. Methods A retrospective analysis was performed for patients with cerebral metastases and complete surgical resection evaluated by an early postoperative MRI < 72h. Minimal ADC and mean ADC were assessed in preoperative 1,5T-MRI scans by placing regions of interests in the tumour and the peritumoural tissue. Results Analysis of the relation between ADC values, local progression and outcome was performed in 86 patients with a mean age of 59 years (range 33–83 years). Primary site was NSCLC in 37.2% of all cases. Despite complete resection 33.7% of all patients suffered from local in-brain-progression. There were no significant differences in ADC values in groups based on histology. In the present cohort, the mean ADCmin and the mean ADCmean within the metastasis did not differ significantly between patients with and without a later local in-brain progression (634 × 10−6 vs. 661 × 10−6 mm2/s and 1324 × 10−6 vs. 1361 × 10−6 mm2/s; 1100 × 10−6 vs. 1054 × 10−6 mm2/s; each p > 0.05). Mean ADC values did not correlate significantly with PFS and OAS. Conclusion In the present study analysed ADC values had no significant impact on local in brain progression and survival parameters.
Randomized Controlled Trial of Scrotal Versus Inguinal Orchidopexy on Postoperative Pain.

INTRODUCTION AND OBJECTIVE To compare the impact of orchidopexy approach(scrotal[SO]vs.inguinal[IO]) on analgesic requirements, postoperative pain scores and complication rates. METHODS A superiority RCT including boys 10-95 months of age at surgery, diagnosed with palpable undescended testis(UDT) was conducted. Patients with non-palpable or bilateral UDT, previous inguinal surgery on the ipsilateral side and concurrent procedures were excluded. Block randomization with 1:1 allocation ratio and a standardized anesthesia protocol were employed. The primary outcome was postoperative pain and analgesic use in hospital and at home using validated pain scales(FLACC,CHEOPS,PPPM,TPPPS). Secondary outcomes included operative time(OpT), conversion and success rates, and complications. An intention to treat protocol(ITT) was followed. RESULTS We enrolled 173 patients, 12 withdrew. Of the161 patients who completed follow-up, 80 had SO and 81 IO. In-hospital use of ibuprofen(p=0.02) and acetaminophen(p<0.01), as well as FLACC(p<0.01) and CHEOPS(p=0.04) pain scores were slightly higher in IO patients. No difference in mean OpT and median at-home administration of analgesic was noted. The conversion rate was 24%(19/80). Of these, 13(68%) had canalicular testes. The overall complication rate was 4%(6/161):1 testicular atrophy, 3 re-ascents, 2 wound infections. Of these, 5 underwent SO and 1 had IO(wound infection). CONCLUSIONS Even though in-hospital mean postoperative pain scores and analgesic consumption were slightly lower for SO patients, the pain levels were mild across all scales. Median at-home analgesic use and pain scores were similar for both groups, as well as OpT and complication rates. SO is an effective alternative to IO for low-lying UDT, as 68%of those that needed conversion were canalicular testes.