Miracle-dz/UniFAR-FTUs huggingface dataset:
Does 5-HTTLPR moderate the effect of the quality of environmental context on maternal sensitivity? Testing the differential susceptibility hypothesis

Evidence documenting associations between 5-HTTLPR and parenting behavior led to testing the hypothesis that this polymorphism moderates the effect of the quality of environmental context on maternal sensitivity. Participants were 210 Portuguese mothers and their preschool children, recruited from the community. An index reflecting the quality of the environmental context was derived based on nine markers (e.g. single parenthood; parental education, economic difficulties, family conflict, maternal psychopathology). Maternal sensitivity was measured observationally. Maternal saliva was collected with OraGene kits for genetic analysis. Results revealed a gene-X-environment interaction, such that short-allele homozygotes proved more sensitive to the family context than long-allele carriers (i.e. sL/LL), displaying the highest and lowest levels of maternal sensitivity, depending on, respectively, low and high quality levels of the environmental context. Because even mothers carrying the long allele evinced similar responsiveness to the environmental context, but to a lesser extent, findings proved consistent with the weak differential susceptibility model of person-X-context interaction. Results are discussed in light of prior and related gene-X-environment findings.
Increased performance of DNA metabarcoding of macroinvertebrates by taxonomic sorting

DNA-based identification through the use of metabarcoding has been proposed as the next step in the monitoring of biological communities, such as those assessed under the Water Framework Directive (WFD). Advances have been made in the field of metabarcoding, but challenges remain when using complex samples. Uneven biomass distributions, preferential amplification and reference database deficiencies can all lead to discrepancies between morphological and DNA-based taxa lists. The effects of different taxonomic groups on these issues remain understudied. By metabarcoding WFD monitoring samples, we analyzed six different taxonomic groups of freshwater organisms, both separately and combined. Identifications based on metabarcoding data were compared directly to morphological assessments performed under the WFD. The diversity of taxa for both morphological and DNA-based assessments was similar, although large differences were observed in some samples. The overlap between the two taxon lists was 56.8% on average across all taxa, and was highest for Crustacea, Heteroptera, and Coleoptera, and lowest for Annelida and Mollusca. Taxonomic sorting in six basic groups before DNA extraction and amplification improved taxon recovery by 46.5%. The impact on ecological quality ratio (EQR) scoring was considerable when replacing morphology with DNA-based identifications, but there was a high correlation when only replacing a single taxonomic group with molecular data. Different taxonomic groups provide their own challenges and benefits. Some groups might benefit from a more consistent and robust method of identification. Others present difficulties in molecular processing, due to uneven biomass distributions, large genetic diversity or shortcomings of the reference database. Sorting samples into basic taxonomic groups that require little taxonomic knowledge greatly improves the recovery of taxa with metabarcoding. Current standards for EQR monitoring may not be easily replaced completely with molecular strategies, but the effectiveness of molecular methods opens up the way for a paradigm shift in biomonitoring.
Cancer Hazard Identification Integrating Human Variability: The Case of Coumarin

Coumarin is a naturally occurring sweet-smelling benzopyrone that may be extracted from plants or synthesized for commercial uses. Its uses include as a flavoring agent, fragrance enhancer, and odor-masking additive. We reviewed and evaluated the scientific evidence on the carcinogenicity of coumarin, integrating information from carcinogenicity studies in animals with mechanistic and other relevant data, including data from toxicogenomic, genotoxicity, and metabolism studies, and studies of human variability of a key enzyme, CYP2A6. Increases in tumors were observed in multiple studies in rats and mice in multiple tissues. Our functional pathway analysis identified several common cancer-related biological processes/pathways affected by coumarin in rat liver following in vivo exposure and in human primary hepatocytes exposed in vitro. When coumarin 7-hydroxylation by CYP2A6 is compromised, this can lead to a shift in metabolism to the 3,4-epoxidation pathway and increased generation of electrophilic metabolites. Mechanistic data align with 3 key characteristics of carcinogens, namely formation of electrophilic metabolites, genotoxicity, and induction of oxidative stress. Considerations of metabolism, human variability in CYP2A6 activity, and coumarin hepatotoxicity in susceptible individuals provide additional support for carcinogenicity concern. Our analysis illustrates the importance of integrating information on human variability in the cancer hazard identification process.
Can High-Intensity Functional Suspension Training over Eight Weeks Improve Resting Blood Pressure and Quality of Life in Young Adults? A Randomized Controlled Trial

The present study examined the effects of a functional high-intensity suspension training (FunctionalHIIT) on resting blood pressure, psychological well-being as well as on upper body and core strength and cardiorespiratory fitness in moderately trained participants. Twenty healthy, moderately trained adults (10 males and 10 females; age: 36.2 ± 11.1 years, BMI: 23.9 ± 3.7) were randomly assigned to a FunctionalHIIT training group or passive control group (CON). FunctionalHIIT performed 16 sessions (2× week for eight weeks, 30 min per session), whereas CON maintained their habitual lifestyle using a physical activity log. Before and after FunctionalHIIT intervention, resting blood pressure and quality of life (short version of the WHO Quality of Life questionnaire (WHOQOL-BREF)) were assessed. Furthermore, maximum-repetition (leg press, chest press, pulldown, back extension) and trunk muscle strength (Bourban test) as well as cardiorespiratory fitness (Vameval test), were measured before and after the intervention. Both systolic and diastolic blood pressure and WHOQOL-BREF did not change significantly but both showed moderate training-induced effects (0.62 < standardized mean difference (SMD) < 0.82). Significant improvements in the FunctionalHIIT group were evident on leg press (p < 0.01), chest press (p < 0.05), and left side Bourban test (p < 0.05). Cardiorespiratory fitness did not reveal any time effects or time × group interactions. The present study revealed that eight weeks of FunctionalHIIT represents a potent stimulus to improve health-related parameters in young adults, whereas FunctionalHIIT was not sufficient to improve cardiorespiratory fitness.
Farmer Burnout in Canada

While farmers in several countries worldwide are reported to be at higher risk for poor mental health outcomes like chronic stress, depression, and anxiety, there is a paucity of research on burnout in farmers. This cross-sectional study used an online survey administered between September 2015 and February 2016 to investigate burnout (as measured by the Maslach Burnout Inventory–General Survey (MBI–GS)) amongst farmers in Canada. The specific objectives were to measure the three components of burnout (exhaustion, cynicism, and professional efficacy), and to explore potential associated risk factors, as well as to determine the prevalence of the different burnout profiles (engaged, ineffective, overextended, disengaged, and burnout). MBI–GS results were obtained from 1075 farmers. Approximately 70% of the study sample identified as male and 30% as female, and participants were from a variety of farming commodities. Scores for exhaustion, cynicism, and professional efficacy were all higher than international norms. While 43% of participants were classified as engaged, 44% were classified in the ineffective, overextended, or disengaged profiles (i.e., intermediate profiles on the engagement – burnout continuum), and 12% were classified in the burnout profile. Risk factor results highlighted the positive effects of farmer support from spouse/romantic partner, friends, and industry. Overall, the results from this study demonstrate cause for concern with respect to farmer burnout, suggest potential avenues for intervention, and serve as a call to action to better support farmers in Canada.
Foliar Moisture Content from the Spectral Signature for Wildfire Risk Assessments in Valparaíso-Chile

Fuel moisture content (FMC) proved to be one of the most relevant parameters for controlling fire behavior and risk, particularly at the wildland-urban interface (WUI). Data relating FMC to spectral indexes for different species are an important requirement identified by the wildfire safety community. In Valparaíso, the WUI is mainly composed of Eucalyptus Globulus and Pinus Radiata—commonly found in Mediterranean WUI areas—which represent the 97.51% of the forests plantation inventory. In this work we study the spectral signature of these species under different levels of FMC. In particular, we analyze the behavior of the spectral reflectance per each species at five dehydration stages, obtaining eighteen spectral indexes related to water content and, for Eucalyptus Globulus, the area of each leave—associated with the water content—is also computed. As the main outcome of this research, we provide a validated linear regression model associated with each spectral index and the fuel moisture content and moisture loss, per each species studied.
Standard Hot Pressing as a Possible Solution to Obtain Dense K0.5Na0.5NbO3 Ceramic Doped by Er2O3 and Yb2O3

In this study, the influence of the addition of rare earth oxides on the phase composition and density of KNN piezoelectric ceramics was investigated. The initial powders of Na2CO3 and K2CO3 were dried at 150 °C for 2 h. Then, a powder mixture for synthesis was prepared by adding a stoichiometric amount of Nb2O5 and 5 and 10 wt % overabundance of Na2CO3. All powders were mixed by ball-milling for 24 h and synthesized at 950 °C. The phase composition of the reaction bed was checked by means of X-ray diffraction (XRD). It had an appearance of tetragonal and monoclinic K0.5Na0. 5NbO3 (KNN) phases. Then, 1 and 2 wt % of Er2O3 and Yb2O3, were added to the mixture. Green samples of 25 mm diameter and 3 mm thickness were prepared and sintered by hot pressing at 1000 °C for 2 h under 25 MPa pressure. The final samples were investigated via scanning electron microscopy (SEM)-energy-dispersive X-ray spectroscopy (EDS), XRD, Rietveld, and ultrasonic methods. Phase analysis showed tetragonal and orthorhombic KNN phases, and a contamination of (K2CO3·1.5H2O) was present. The obtained KNN polycrystals had a relative density above 95%. Texturing of the material was confirmed as a result of hot pressing.
Parkinson's disease‐associated dyskinesia in countries with low access to levodopa‐sparing regimens

In their recent viewpoint publication “Should There Be Less Emphasis on Levodopa-Induced Dyskinesia in Parkinson’s Disease,” Chaudhuri et al presented convincing evidence to support the notion that the majority of patients with levodopainduced dyskinesias do not find these symptoms to be troublesome. Furthermore, they found that patients who do experience severe dyskinesias typically respond well to adjustments in dopaminergic therapy and/or the addition of amantadine. Although the authors focused on evidence from patients in developed countries, which are more likely to use agents other than levodopa, they acknowledged that the global burden of dyskinesia may remain a significant issue, especially “in countries where longer-acting therapies or levodopa sparing options may not be available. ” In an attempt to examine the hypothesis raised by the authors regarding the association between severe dyskinesia and the availability of agents other than levodopa, we first reviewed the results from the United States–based BioFIND cohort. In this cohort more than 50% of patients were on dopamine agonists, and approximately 40% experienced dyskinesias (Table 1). However, none of these patients endorsed symptoms of “troublesome dyskinesias” according to the criteria proposed by Chaudhuri et al based on the ALTHEA study (dyskinesias >75% of the waking day and with severe impact on daily function; Table 1). In addition, we report results from our cohort of patients from the Hospital de Clínicas de Porto Alegre (HCPA) in southern Brazil, which also consisted of patients with moderate to advanced PD. All patients who received care in this tertiary-care medical center were enrolled in the Sistema Único de Saúde (SUS), the nationally funded universal health care system. Standard levodopa is readily accessible through SUS; however, agents other than levodopa are often more difficult to obtain. Patients were included in the analysis if treatment information was available within 3 months of a formal neurologic evaluation (UPDRS). Patients were excluded if they were not on antiparkinsonian medications or had missing UPDRS data. Of the 222 patients included in the HCPA cohort, the average age standard deviation (SD) was 67 10.8 years, and the average disease duration SD was 9.1 5.1 years (Table 1). Although these parameters were comparable to the US-based cohort, fewer than 25% of the Brazilian patients were treated with dopamine agonists or amantadine. The overall prevalence of dyskinesias paralleled the US-based cohort, but Brazilian patients with dyskinesias were more than twice as likely to report dyskinesias impacting daily function (63.0%). Despite this difference, when considering the aforementioned markers of “troublesome dyskinesia,” the prevalence was still low. Only 16.0% of affected patients reported experiencing dyskinesias for >75% of the waking day, and 3.7% of affected patients reported dyskinesias with severe impact on daily activities and social interaction. There were no patients who endorsed both of these features of troublesome dyskinesias.
Incidence of Venous Thromboembolism in Patients with Newly Diagnosed Pancreatic Cancer and Factors Associated With Outcomes.

BACKGROUND & AIMS Pancreatic ductal adenocarcinoma (PDAC) is associated with the highest incidence of venous thromboembolism (VTE) of any cancer type. However, little is known about risk factors for VTE or its outcomes in patients with PDAC. METHODS We collected data from a prospective, observational study performed at multiple centers in France from May 2014 through November 2018 (the Base Clinico-Biologique de l'Adénocarcinome Pancréatique [BACAP] study) linked to a database of patients with a new diagnosis of PDAC of any stage. Data were collected from 731 patients at baseline and during clinical follow-up or in the event of symptoms. The primary endpoint was the onset of VTE during follow up. The secondary end points were progression-free survival (PFS) and overall survival (OS) times. RESULTS During a median follow-up of 19.3 months, 152 patients (20.79%) developed a VTE. The median time from PDAC diagnosis to the onset of VTE was 4.49 months. Cumulative incidence values of VTE were 8.07% (95% CI, 6.31-10.29) at 3 months and 19.21% (95% CI, 16.27-22.62) at 12 months. In multivariate analysis, PDAC primary tumor location (isthmus vs head, hazard ratio [HR], 2.06; 95% CI, 1.09-3.91; P=.027) and stage (locally advanced vs resectable or borderline HR, 1.66; 95% CI 1.10-2.51, P=.016 and metastatic vs resectable or borderline HR, 2.50; 95% CI, 1.64-3.79; P<.001) were independent risk factors for onset of VTE. Patients who developed VTE during follow up had shorter times of PFS (HR, 1.74; 95% CI, 1.19-2.54; P=.004) and OS (HR, 2.02; 95% CI, 1.57-2.60; P<.001). CONCLUSION In an analysis of data from the BACAP study, we found that frequent and early onset of VTE after diagnoses of PDAC are associated with significant decreases in times of PFS and OS. Studies are needed to determine whether primary prophylaxis of VTE in patients with PDAC will improve morbidity and mortality related to VTE.
High-Sensitivity C-Reactive Protein and Acute Kidney Injury in Patients with Acute Myocardial Infarction: A Prospective Observational Study

Background. Accumulating evidence suggests that inflammation plays a key role in acute kidney injury (AKI) pathogenesis. We explored the relationship between high-sensitivity C-reactive protein (hs-CRP) and AKI in acute myocardial infarction (AMI). Methods. We prospectively included 2,063 AMI patients in whom hs-CRP was measured at admission. AKI incidence and a clinical composite of in-hospital death, cardiogenic shock, and acute pulmonary edema were the study endpoints. Results. Two-hundred-thirty-four (11%) patients developed AKI. hs-CRP levels were higher in AKI patients (45 ± 87 vs. 16 ± 41 mg/L; p < 0.0001). The incidence and severity of AKI, as well as the rate of the composite endpoint, increased in parallel with hs-CRP quartiles (p for trend <0.0001 for all comparisons). A significant correlation was found between hs-CRP and the maximal increase of serum creatinine (R = 0.23; p < 0.0001). The AUC of hs-CRP for AKI prediction was 0.69 (p < 0.001). At reclassification analysis, addition of hs-CRP allowed to properly reclassify 14% of patients when added to creatinine and 8% of patients when added to a clinical model. Conclusions. In AMI, admission hs-CRP is closely associated with AKI development and severity, and with in-hospital outcomes. Future research should focus on whether prophylactic renal strategies in patients with high hs-CRP might prevent AKI and improve outcome.
Classic Bioelectrical Impedance Vector Reference Values for Assessing Body Composition in Male and Female Athletes

Bioimpedance standards are well established for the normal healthy population and in clinical settings, but they are not available for many sports categories. The aim of this study was to develop reference values for male and female athletes using classic bioimpedance vector analysis (BIVA). In this study, 1556 athletes engaged in different sports were evaluated during their off-season period. A tetrapolar bioelectrical impedance analyzer was used to determine measurements of resistance (R) and reactance (Xc). The classic BIVA procedure, which corrects bioelectrical values for body height, was applied, and fat-free mass, fat mass, and total body water were estimated. In order to verify the need for specific references, classic bioelectrical values were compared to the reference values for the general male and female populations. Additionally, athletes were divided into three groups: endurance, velocity/power, and team sports. In comparison with the general healthy male and female populations, the mean vectors of the athletes showed a shift to the left on the R–Xc graph. Considering the same set of modalities, BIVA confidence graphs showed that male and female endurance athletes presented lower body fluids, fat mass, and fat-free mass than other sets of modalities. This study provides BIVA reference values for an athletic population that can be used as a standard for assessing body composition in male and female athletes.
Whipworm Infection Promotes Bacterial Invasion, Intestinal Microbiota Imbalance, and Cellular Immunomodulation

Infections with Trichuris trichiura are among the most common causes of intestinal parasitism in children worldwide, and the diagnosis is based on microscopic egg identification in the chronic phase of the infection. During parasitism, the adult worm of the trichurid nematode maintains its anterior region inserted in the intestinal mucosa, which causes serious damage and which may open access for gut microorganisms through the intestinal tissue. ABSTRACT Infections with Trichuris trichiura are among the most common causes of intestinal parasitism in children worldwide, and the diagnosis is based on microscopic egg identification in the chronic phase of the infection. During parasitism, the adult worm of the trichurid nematode maintains its anterior region inserted in the intestinal mucosa, which causes serious damage and which may open access for gut microorganisms through the intestinal tissue. The immune-regulatory processes taking place during the evolution of the chronic infection are still not completely understood. By use of the Swiss Webster outbred mouse model, mice were infected with 200 eggs, and tolerance to the establishment of a chronic Trichuris muris infection was induced by the administration of a short pulse of dexamethasone during nematode early larval development. The infected mice presented weight loss, anemia, an imbalance of the microbiota, and intense immunological cell infiltration in the large intestine. It was found that mice have a mixed Th1/Th2/Th17 response, with differences being found among the different anatomical locations. After 45 days of infection, the parasitism induced changes in the microbiota composition and bacterial invasion of the large intestine epithelium. In addition, we describe that the excretory-secretory products from the nematode have anti-inflammatory effects on mouse macrophages cultured in vitro, suggesting that T. muris may modulate the immune response at the site of insertion of the worm inside mouse tissue. The data presented in this study suggest that the host immune state at 45 days postinfection with T. muris during the chronic phase of infection is the result of factors derived from the worm as well as alterations to the microbiota and bacterial invasion. Taken together, these results provide new information about the parasite-host-microbiota relationship and open new treatment possibilities.
A Comparative Study on the Diagnostic Utility of Corneal Confocal Microscopy and Tear Neuromediator Levels in Diabetic Peripheral Neuropathy

ABSTRACT Aims To determine the utility of corneal confocal microscopy and tear neuromediator analysis in the diagnosis of diabetic peripheral neuropathy (DPN) as a result of type 1 and type 2 diabetes. Methods Seventy individuals with either type 1 diabetes or type 2 diabetes (T1D/T2D) underwent corneal confocal microscopy to assess the corneal nerve morphology. The concentration of substance P and calcitonin gene-related peptide (CGRP) in tears was measured by enzyme-linked immunosorbent assay. Motor excitability studies were conducted on the median nerve to assess axonal ion channel function. Based on total neuropathy score (TNS), participants were stratified into DPN (DPN+ve; TNS ≥ 2; T1D, n = 19; T2D, n = 16) and without DPN (DPN–ve; TNS ≤ 1; T1D, n = 19; T2D, n = 16). Areas under the receiver operating characteristic curves (AUCs) were calculated to obtain specificity and sensitivity of the measures to diagnose DPN. Results In T1D, the concentration of substance P and confocal microscopy measures were significantly reduced (P < .010) in DPN+ve. Also, for the nerve excitability measures, mean peak response, percentage of threshold electrotonus at peak and after 90-100 ms, superexcitability and subexcitability were significantly reduced (P < .050) in DPN+ve. In T2D, except for inferior whorl length (P = .190), all other corneal confocal microscopy measures were significantly reduced (P < .010) in DPN+ve, but there was no difference in substance P concentration. For the diagnosis of DPN in T1D, the AUC for inferior whorl length (0.910), mean peak response (0.800) and concentration of substance P (0.770) were high and in T2D, the AUC for corneal nerve fiber length (0.809) and nerve fractal dimension (0.777) were high. Conclusion Corneal confocal microscopy parameters provide a better diagnostic ability to detect DPN in T1D and T2D than nerve excitability measures or concentrations of tear neuromediators. The concentration of substance P could also be useful in diagnosing DPN but for T1D only.