Miracle-dz/UniFAR-FTUs huggingface dataset:
Understanding Restrictive Versus Liberal Fluid Therapy for Major Abdominal Surgery Trial Results: Did Liberal Fluids Associate With Increased Endothelial Injury Markers?

Supplemental Digital Content is available in the text. Objectives: Liberal fluid strategies in critically ill patients are associated with harm, thought to be due to endothelial and glycocalyx injury. As the restrictive versus liberal fluid therapy for major abdominal surgery trial not only failed to report survival benefit with restrictive fluids but was associated with a higher rate of acute kidney injury, we hypothesized that factors other than endothelial and glycocalyx injury were likely to account for these findings. Consequently, we measured injury biomarkers in a cohort of the restrictive versus liberal fluid therapy for major abdominal surgery trial. Design: The restrictive versus liberal fluid therapy for major abdominal surgery trial was an international, randomized, assessor-blinded trial comparing restrictive with liberal IV fluid regimens that represented traditional care in patients undergoing major abdominal surgery. Setting and Patients: Cohort of restrictive versus liberal fluid therapy for major abdominal surgery bloods was collected at a single major site (161 patients) prior to, day 1 and day 3 after surgery. Intervention: Bloods were blindly and randomly batch analyzed for plasma markers of endothelial/glycocalyx injury—angiopoietin-1, angiopoietin-2, soluble tyrosine-protein kinase-2 receptor, soluble intracellular adhesion molecule-1, syndecan, and tumor necrosis factor-α. Data were examined as restrictive versus liberal enrollment groups and high versus low (± 5,000 mL) fluid groups. Differences were examined by linear mixed modeling. Measurement and Main Results: There were no significant differences in any biomarkers between the restrictive (n = 75) and liberal (n = 86) groups. When examined as low (n = 81) and high (n = 79) fluid groups, plasma angiopoietin-2 (p = 0.009) and soluble intracellular adhesion molecule-1 (p = 0.01) were elevated in the high fluid group. There were no differences in other biomarkers. Conclusions: Although these results are consistent with previous findings of vascular injury following liberal fluid therapy, they suggest alternative mechanisms underlie the clinical outcomes from restrictive versus liberal fluid therapy for major abdominal surgery study. ClinicalTrials.gov identifier: NCT01424150.
INVESTIGATION OF ACID DEPOSITION EFFECTS ON SOUTHERN APPALACHIAN RED SPRUCE (Picea rubens) BY DETERMINATION OF CALCIUM, MAGNESIUM, AND ALUMINUM IN FOLIAGE AND SURROUNDING SOIL USING ICP-OES

Red spruce (Picea rubens) are conifers found at high elevations (above 1370 m) sites in the Southern Appalachian Mountains. A decline of red spruce forests in the Southern Appalachian Mountains caused by atmospheric acid deposition has been reported since the 1970s. Acid deposition leaches essential nutrients (calcium and magnesium) out of the soil and increases the availability of toxic metals (aluminum) to plants. Acid deposition effects on red spruce forests were investigated by determining aluminum, calcium, and magnesium in foliage and surrounding soils using inductively coupled plasma optical emission spectrometry (ICP-OES). There was some correlation in nutrient or toxic metal concentrations found in the foliage or surrounding soils of red spruce trees with respect to elevation and geography of red spruce forests located in the Southern Appalachian Mountains. The majority of the results indicated that foliar nutrient or toxic metal concentrations from red spruce trees did not correlate with soil metal concentrations. Life stage of red spruces trees were independent of foliar or soil metal concentrations. A previously developed model using soil calcium/aluminum molar ratios suggested that almost all sample sites located in the Southern Appalachian Mountains are at high risk of adverse forests health effects. A comparison of red spruce sapling foliar calcium/aluminum ratios at Clingman's Dome, North Carolina/Tennessee, suggested a possible improvement since the 1980s in red spruce forest health. A comparison of sapling red spruce foliar calcium and magnesium concentrations with previous studies, which spanned 40 years, at Richland Balsam, North Carolina, suggested an improvement in red spruce health since 1994.
Prevalence of Microalbuminuria in Type 2 Diabetes Mellitus

Objective: The presence of albumin in the urine is a marker of glomerular involvement in type 2 diabetes mellitus (T2DM), depicting diabetic nephropathy. Strict glycemic control can prevent and delay the occurrence of microalbuminuria and other diabetic complications. Therefore, we conducted a study to report the prevalence of microalbuminuria in type 2 diabetics along with its association with diabetic control. Methods: A total of 133 patients with T2DM were consecutively included and their co-morbidities, body mass index, mode of treatment of diabetes (oral hypoglycemic drugs and/or insulin), duration since diagnosis of T2DM, and hemoglobin A1c (HbA1c) levels were recorded. A morning, mid-stream urine sample was collected and a urine spot for albumin:creatinine ratio (UACR) was assessed. Descriptive and analytic statistics were drawn with different variables and UACR values. Results: The mean age of the participants was 54.5 ± 10.3 years which included 60.9% males and 39.1% females. The overall incidence of diabetic nephropathy was 30.1%, with 25.6% having microalbuminuria and 4.5% having macroalbuminuria. Pearson correlation test was used to compare UACR and duration of diabetes (p=0.034) and HbA1c (p=0.001). Conclusion: UACR was higher in patients with uncontrolled T2DM (in terms of higher HbA1c value) and with a longer duration since diagnosis. We recommend that UACR should be inculcated in routine practice, annually, for all patients with T2DM for gauging the development of underlying renal involvement and prompt management.
Small-angle neutron scattering from hexadecyltrimethylammonium bromide micelles in aqueous solutions

Small-angle neutron scattering measurements have been carried out at 50/sup 0/C as a function of external contrast (different ratios of H/sub 2/O to D/sub 2/O) on 0.12 M solutions of hexadecyltrimethylammonium bromide, with the methyls of the ammonium group protiated and deuterated. The results indicate that most of the surfactant hydrocarbon is in a micellar core penetrated by little, if any, solvent. The micelles are described adequately by a dispersion of monodisperse prolate ellipsoids. There is a significant effect on the size of the aggregates by the isotopic composition of the solvent.
Prenatal diagnosis of fetal congenital mesoblastic nephroma by ultrasonography combined with MR imaging

Abstract Rationale: Fetal congenital mesoblastic nephroma (CMN) is a rare renal tumor, characterized by polyhydramnios, premature birth, and neonatal hypertension. In the prenatal stage, it is particularly difficult to diagnose CMN either by ultrasonography or magnetic resonance imaging (MRI). Thus, CMN is frequently detected in the third trimester in the clinical scenario. Patient concerns: A 29-year-old G2P0 pregnant woman took routine prenatal examinations in our hospital. The fetal right kidney abnormality was not observed after 2 systematical ultrasonic examinations (at 24 and 31 weeks of gestation respectively), and only an increase was noticed in the amniotic fluid index (from 19.3 to 20.8 cm). Diagnosis: CMN was detected by antenatal ultrasonography and MRI as a fetal right renal mass at 35 weeks of gestation in our hospital. Interventions: The pregnant woman was admitted at a gestational age of 38 weeks and 5 days due to alterations in renal function. Further, the pregnant woman was administered with “oxytocin” to promote delivery, and the neonate underwent a right nephrectomy on the 9th day after birth. Outcomes: The pathological examination confirmed a cellular type of right CMN. The neonate recovered well after operation without adjuvant treatment. During 6 months of follow-up, the neonate grew well and showed no signs of recurrence or metastasis. Conclusion: Polyhydramnios detected during prenatal examination required attention due to the risk of malformation of fetal urinary system. Prenatal ultrasonography combined with MRI could not only clearly identify the origin of the tumor, but also distinguish the correlation between the tumor and adjacent structures, thereby leading to early diagnosis and favorable prognosis.
Records of ctenophores from South Africa

Although ctenophores can be conspicuous components of the plankton in coastal marine ecosystems, only six species have been formally described from around South Africa. Using photographs from local community scientists, we add a further three species (Cestum veneris, Beroe forskalii? , Ocyropsis maculata? ) and six morphospecies to the regional fauna. These additions suggest that South Africa has a ctenophore fauna that is amongst the most diverse, globally; an observation in agreement with information from other taxa. Tips on how community scientists can improve their photographic contributions to understanding ctenophore diversity are provided.
High molecular prevalence of HPV and other sexually transmitted infections in a population of asymptomatic women who work or study at a Brazilian university

ABSTRACT Sexually transmitted infections (STIs) represent a global health problem with variable prevalence depending on the geographical region and the type of population. Human papillomavirus (HPV) encompasses widespread virus types related to cervical carcinogenesis. The present study investigated the molecular prevalence of HPV and seven other important STIs in asymptomatic women working or studying at a Brazilian university. A secondary aim was to assess cytological abnormalities associated with HPV and other STIs coinfections. We recruited 210 women from a Brazilian university. HPV was detected using a single-round polymerase chain reaction (sPCR) followed by a viral genotyping by restriction fragment length polymorphism (RFLP-PCR). The presence of seven STIs: Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, herpes simplex virus (HSV)-1 and HSV-2 was detected by multiplex PCR (M-PCR). Furthermore, cytological findings and epidemiological characteristics were evaluated.The mean age of the participants was 27.1 years old. HPV prevalence was 33.8%, and HPV16 was the most frequently detected papillomavirus genotype. Moreover, multiple HPV infections were common (42.2%). We detected at least one STI agent in 11.4% of the tested women, most frequently C. trachomatis (6.7%). Among HPV-positive women, 14.1% were coinfected with other STI agents. Cytological abnormalities were observed in 9.5% of smears, and HPV-DNA, high-risk HPV (HR-HPV), HPV16 and HPV multiple infections were associated with abnormal cytological findings. There was a high prevalence of HPV, and C. trachomatis was the most prevalent STI agent, with low rates of cytological abnormalities. These findings highlight the need of timely STI diagnosis in young asymptomatic women and of a public policy design for STI prevention.
Correlation Between Facial Skin Microbiota and Skin Barriers in a Chinese Female Population with Sensitive Skin

Background and Aim The association of microbiota changes with sensitive skin remains controversial until now. Although a strong correlation is detected between skin microbiota distribution and biophysical parameters, there is little knowledge on the link between sensitive skin and skin microbiota in Chinese women. This study aimed to unravel the correlation between facial skin microbiota distribution and skin barriers in Chinese women with sensitive skin. Materials and Methods In total, 34 volunteers were enrolled, including 24 subjects with sensitive skin (SS group) and 10 subjects with non-sensitive skin (NS group). The cuticle moisture content, transepidermal water loss (TEWL), and facial skin sebum secretion were measured, and the facial skin surface morphology was evaluated. Sensitive skin samples were collected from the facial (SS-F group) and chest skin of subjects in the SS group (SS-C group), while non-sensitive skin samples were collected from the facial skin of subjects in the NS group (NS-F group). All skin samples were subjected to 16S rRNA sequencing. Results 16S rRNA sequencing detected Actinobacteria, Firmicutes, and Proteobacteria as the three most common microbiota phyla and Propionibacterium, Paracoccus, and Corynebacterium as the three most common microbiota genera, and there were no significant differences in the relative frequency of Actinobacteria, Firmicutes, or Proteobacteria, or Propionibacterium, Paracoccus, or Corynebacterium among the SS-F, SS-C, and NS-F groups (P>0.05). We detected no significant difference in the diversity of bacterial communities among the SS-F, SS-C, and NS-F groups; however, the Shannon’s diversity index was significantly higher in the NS-F group than in the SS-C group. In addition, Spearman correlation analysis showed a correlation between the microbiota genera and skin physiological parameters (P<0.05). Conclusion This study preliminarily unravels the skin microbiota of sensitive skin using a high-throughput tool, and there are no microbiota genera with strong associations with skin physiological parameters.
Effect of Simultaneous Integrated Boost Intensity Modulated Radiation Therapy (SIB-IMRT) and Non-Operative Strategy on Outcomes of Distal Rectal Cancer Patients with Clinically Positive Lateral Pelvic Lymph Node

Background We aimed to analyze the effect of simultaneous integrated boost intensity modulated radiation therapy (SIB-IMRT) and non-operative treatment on the clinical outcomes of distal rectal cancer patients exhibiting clinically positive lateral pelvic lymph nodes (LPLNs). Methods We reviewed the medical records of patients diagnosed as having distal rectal adenocarcinoma with clinically positive LPLNs (≥7 mm, with irregular borders or mixed signal intensity) using primary pelvic magnetic resonance imaging (MRI). These patients had received SIB-IMRT-based neoadjuvant chemoradiotherapy (NCRT) and non-operative treatment according to the heterogeneity of the disease or personal preference. Chi-square tests were used to compare data between the two groups. Progression-free survival (PFS) and local regrowth were evaluated using the Kaplan-Meier method. Results Between 2016 and 2019, we analyzed 75 patients diagnosed as having clinically positive LPLNs using primary MRI. SIB-IMRT was delivered to the planning positive LPLNs (PGTVn) at a total dose of 56–60 Gy. After NCRT, 23 patients underwent non-operative treatment. Among these patients, the median short axis of LPLNs was 8 mm (range: 7–21 mm). Fifteen patients were categorized into the mesorectal fascia (MRF)-positive group. The median follow-up duration for these patients was 19.8 months, and no patient exhibited LPLN regrowth. The 2-year PFS rate was 85.6% for non-operative patients, 74.6% for operative patients, and 90.0% for the pathological complete response (pCR) subgroup. Eighteen patients who underwent non-operative treatment were included in the clinical complete response (cCR) subgroup. The 2-year PFS and local regrowth rates in this group were similar to those in patients with clinically negative LPLN who achieved cCR. During NCRT, 21 (28.0%) patients experienced grade 2–3 acute reversible toxicity. Conclusions SIB-IMRT could eliminate metastases in LPLNs in a safe and effective manner, and non-operative strategies may be promising for cCR patients.
Comparison of Liquid-Based Cytology and Conventional Papanicolaou Smear for Cervical Cancer Screening: An Experience From Pakistan

Introduction After the introduction of cervical cancer screening program with cervical cytology, a marked decline in deaths secondary to cervical cancer was observed in developed countries. Two methods are used for cervical cytology. The first one is the conventional Papanicolaou (PAP) and the second one is liquid-based cytology (LBC). Although various studies in western countries established the role of LBC in cervical cancer screening, no large-scale study was conducted in our population to compare the two techniques for cervical cancer screening. Therefore, in this study, we compared the diagnostic utility of these two techniques for detecting cervical epithelial lesions. Methods A total of 3,929 patients, who presented to the Gynecology Clinic, Liaquat National Hospital, for cervical cancer screening from January 2015 until December 2019, over a period of five years, were included in the study. A total of 1,503 specimens were prepared by LBC, and 2,426 specimens were prepared by a conventional PAP smear method. All smears were interpreted using the Bethesda System of Reporting Cytopathology. Results The mean age of the patients was 39.46±11.14 years. For cytological evaluation, 98.7% of specimens were adequate. The inadequacy rate was 1.3% for conventional PAP smear and 1.2% for LBC. While 97.2% of specimens were reported as negative for intraepithelial lesion or malignancy, 1.1% of specimens showed squamous epithelial lesions. There was a significant difference in the detection rate of squamous epithelial lesions using the two techniques. The detection rate of squamous intraepithelial lesions using LBC was 2.1%, which was higher than that of the conventional PAP smear (0.6%). The detection rates of glandular lesions using LBC and conventional PAP smear were 0.5% and 0.2%, respectively. Conclusion We found a higher disease detection rate of squamous epithelial lesions using LBC compared to conventional PAP smear. Therefore, we recommend a widespread use of LBC for mass cervical cancer screening in our population.
Anticancer Activity of Kefir on Glioblastoma Cancer Cell as a New Treatment

Kefir drink is one of the most important probiotic products, which is made using kefir microorganisms in fermenting the milk. Numerous investigation have been accomplished in the field of the therapeutic property of probiotic products. In the present study, we assessed the cytotoxic effect of kefir on the rate of growth and increase of glioblastoma cancer cell as the most severe form of brain tumors. In this experimental study, we used a U87 cancer cell line (glioblastoma). The interaction between cancer cells and different concentrations of kefir drink and supernatants at 24 and 48 hours was considered. The cell cytotoxicity of kefir and sedimentation of cell lysate and extract of kefir was assessed using the MTT test after 24 and 48 hours. The result of the MTT test, treatment of the cells with the 48-hour fermented drink, demonstrated the most cell cytotoxicity in comparison with the control group. Results showed that the toxicity effect in all groups was dose-dependent, and by increasing the concentration, cell survival decreased noticeably. The results indicated that the supernatant of fermented kefir drink as a probiotic product has more toxicity and lethality effect on the glioblastoma cancer cell. This product can be utilized as a replacement or a complementary therapy of cancer.
Beneficial Effects of Algerian Green Alga Ulva lactuca and Its Hydroethanolic Extract on Insulin Resistance and Cholesterol Reverse Transport in High-Fat/Streptozotocin Diabetic Rats

The aim of this study was to evaluate the impact of the green algae Ulva lactuca and its hydroethanolic extract on insulin resistance and cholesterol reverse transport in type 2 diabetic (T2D) rats. Rats had T2D induced by a high-fat diet (HFD) for 5 weeks followed by intraperitoneal injection of streptozotocin. Diabetic rats were divided into three groups and were fed a HFD in the presence or absence of 1% alga (HFD-Alg) or 1% of its hydroethanolic extract (HFD-Ext), for 4 weeks. The control group consumed 20% casein combined with 5% lipids. Hyperglycemia, insulin resistance, hypercholesterolemia, and hypertriglyceridemia were noted in HFD rats vs control rats. Whole alga and its extract decreased these parameters vs the HFD. Moreover, fecal total cholesterol and triacylglycerols levels were lowered in HFD group vs C group, but were increased with HFD-Alg vs HFD. Compared with the Control, the HFD group had decreased lecithin:cholesterol acyltransferase (LCAT) activity, apolipoprotein A-I (ApoA-I), high-density lipoprotein (HDL3)-phospholipids (PL), and HDL2-cholesteryl ester (CE) levels, but increased HDL3-unesterified cholesterol (UC) levels. Furthermore, compared with the HFD group, the HFD-Alg and HFD-Ext groups had increased LCAT activity, ApoA-I, HDL3-PL, and HDL2-CE levels and decreased HDL3-UC levels. In addition, in the HFD-Ext group, LCAT activity and ApoA-1 levels were decreased vs the HFD-Alg whereas HDL3-UC levels were increased. In conclusion, these results indicate that U. lactuca and its hydroethanolic extract have curative effect on T2D. Therefore, this alga could be considered a functional food supplement for the treatment and prevention of diabetes.
Identification of the Range of Nursing Skills Used to Provide Social Support for Mothers of Preterm Infants in Neonatal Intensive Care

Background Hospitalization of preterm infants in neonatal intensive care units (NICUs) is a stressful experience for parents. Iranian NICUs do not have specified levels of care, nor do they integrate supportive methods of parent support such as family-centered care approaches. This study investigated the range and types of neonatal nursing support, as perceived by mothers of preterm infants, and its association with mothers' satisfaction with infant care in the NICU. Methods This is a descriptive, correlational study of mothers of preterm infants who were hospitalized in three different NICUs in Iran. A convenience sampling method was used. Data were collected using three questionnaires that identified (i) demographic information; (ii) social support available; and (iii) parent satisfaction with infant care. Results Mothers (N = 110) generally rated the support from nurses as being moderate. Correlation analysis identified a moderate association of neonatal nurse social support domains for affirmational (r = 0.44) and concrete aid (r = 0.41), a moderately strong association for affectional support (r = 0.64), and total social support (r = 0.60) with mothers' satisfaction. Conclusion There were positive associations between social support from nurses and mothers' satisfaction with the care of their infants. Therefore, planning to promote and create opportunities for neonatal nurses to support mothers in NICU is important to promote increased maternal satisfaction in infant care.