Miracle-dz/UniFAR-FTUs huggingface dataset:
Profiling of Human Gut Virome with Oxford Nanopore Technology

Human gut virome play critical roles in maintaining gut microbial composition and functionality, as well as host physiology and immunology. Yet, there are insufficient amount of studies on this topic mainly due to methodological limitations, including enrichment of viruses (phages and host viruses) as well as short read-length from current sequencing technology. Here we developed a full working protocol for analyzing human gut virome using physical enrichment, reverse transcription and random amplification, and eventually the state-of-art single-molecule real-time sequencing (SMRT) platform of Oxford Nanopore Technology (ONT). We demonstrate that sequencing viral DNA directly, or viral DNA/cDNA after amplification using ONT achieves much longer reads and provides more information regarding virome diversity, many of the virome sequences do not have match in current databases. Moreover, direct DNA sequencing of virome provides first insights into the epigenetic modifications on phages, where signals of methylations can be directly detected. Our study demonstrates that progressing sequencing technology and bioinformatic improvements will bring more knowledge into virome composition, diversity and potentially their important functions. Highlights Virus-like particles were enriched from human stool samples; Viral nucleotides were sequenced with Oxford Nanopore Technology with and without amplification; Gut virome in humans showed highly individualized composition; Novel sequences and contigs were found to be the majority in the resulted sequences; Epigenetic modifications were detected directly on virus genomes.
Comparative Analysis of Constraint Handling Techniques for Constrained Combinatorial Testing

Constraints depict the dependency relationships between parameters in a software system under test. Because almost all systems are constrained in some way, techniques that adequately cater for constraints have become a crucial factor for adoption, deployment and exploitation of Combinatorial Testing (CT). Currently, despite a variety of different constraint handling techniques available, the relationship between these techniques and the generation algorithms that use them remains unknown, yielding an important gap and pressing concern in the literature of constrained combination testing. In this article, we present a comparative empirical study to investigate the impact of four common constraint handling techniques on the efficiency of six representative (greedy and search-based) test suite generation algorithms. The results reveal that the Verify technique implemented with the Minimal Forbidden Tuple (MFT) approach is the fastest, while the Replace technique is promising for producing the smallest constrained covering arrays, especially for algorithms that construct test cases one-at-a-time. The results also show that there is an interplay between efficiency of the constraint handler and the test suite generation algorithm into which it is developed.
The Utilization of Mobile Learning in Junior High School Physics Science Learning in Rural Area of Sikka, East Nusa Tenggara, Indonesia

: People are the widely used mobile technology for their activities in various sectors. This technology also is widely considered for use in education; included in science learning. On the other hand, almost all people in many regions in Indonesia are connected through the internet network. However, the use of mobile technology for learning is still the islands around the Flores area. This study aims to determine how high school students respond after attending mobile learning. This research is a quantitative descriptive study with a sample of 58 students from three different schools at Sikka of East Nusa Tenggara in Indonesia. Students are involved in mobile learning in material physics within two weeks. Learning active ies include reading learning subjects, practicing questions, discussion in forums, posting ideas, quizzes, and final examinations. During this learning, the teacher used Edmodo, like a learning management system. Students filled the questionnaire measuring their response to the use of technology. The USE questionnaire was used to measure this response. The results of this study indicate that students in this region have the right level of acceptance of mobile learning. As a follow-up, it needs to be promoted by local government policies on schools for this mobile learning.
Daylight photodynamic therapy: clinical and aesthetic benefit with repeated sessions for facial actinic keratoses

Daylight photodynamic therapy (daylight-PDT) has been used in the treatment of facial and scalp actinic keratoses (AKs) as an alternative of similar efficacy but with greater tolerance than the conventional form of the procedure (c-PDT). Twelve patients performed 2 sessions of daylight-PDT with 30-day intervals without major adverse events. Improvement of AKs and photorejuvenation were observed. Repeated sessions of daylight-PDT are well tolerated and may increase clinical and aesthetic benefit in patients with face and scalp AKs.
Estado de salud bucal en pacientes discapacitados del área de salud de Espartaco, municipio Palmira

Fundamento: las personas discapacitadas presentan condiciones que limitan sus posibilidades para desarrollar la vida cotidiana y por consiguiente requieren de una atencion de salud integral; sin embargo se considera que a nivel mundial la mayoria de esta poblacion no recibe atencion bucodental acorde a sus requerimientos. Objetivo: determinar el estado de salud bucal en pacientes discapacitados. Metodos: estudio de serie de casos realizado de junio a septiembre de 2015, sobre 88 discapacitados que cumplieron con los criterios de inclusion. Se analizo: edad, sexo, nivel de escolaridad, ocupacion, clasificacion epidemiologica en cuanto a salud bucal, problemas de salud bucal, enfermedades cronicas, factores de riesgo, tratamientos realizados en atencion primaria y secundaria. Resultados: predomino el sexo masculino sobre el femenino y el grupo de edades de 35 a 59 anos; prevalecieron los tecnicos medios y los de nivel preuniversitario; el 38, 6 % poseia empleo. Fueron mas numerosas las discapacidades mentales. El 72, 2 % padecia de hipertension arterial. La clasificacion epidemiologica del estado de salud bucal mostro que 54, 5 % estaban enfermos; 19, 3 % sanos con riesgo y 26,1 % deficientes discapacitados. Dentro de los factores de riesgo sobresalieron los antecedentes de caries y cepillado incorrecto. En atencion primaria predominaron las restauraciones (47,7 % ) y en la atencion secundaria la ortodoncia (13,6 %). Conclusiones: la salud bucal en el grupo estudiado fue deficiente, lo que pudiera estar dado porque tanto pacientes como familiares dan mas importancia a la condicion fisica como discapacitado que a la salud bucal.
Hepatic Arterial Infusion Chemotherapy with Cisplatin for TACE-Refractory or -Ineligible Hepatocellular Carcinoma with Child-Pugh Score of 8 or Above

Background: This study aimed to retrospectively assess the safety and usefulness of hepatic arterial infusion chemotherapy (HAIC) with cisplatin in patients with hepatocellular carcinoma (HCC) and Child-Pugh (C-P) score ≥ 8, who were refractory to or ineligible for transcatheter arterial chemoembolization (TACE). Methods: In this study, 28 cisplatin-naive patients with HCC, C-P score ≥ 8, and no evidence of extrahepatic lesions were treated using HAIC with cisplatin between July 2004 and July 2013. Results: Of 28 patients, 10 were refractory to TACE and 18 ineligible for TACE. In terms of C-P score, 17 patients had a score of 8, 6 a score of 9, and 5 a score of 10. The injected dose of cisplatin was reduced in 64.3% of cases. The overall response rate was 10.7%, with a disease control rate of 35.7%. Overall, median survival time (MST) and progression-free survival were 186 and 80 days, respectively. In patients with macroscopic vascular invasion (MVI-positive; n = 9), these values decreased to 161 and 72 days, respectively; while they increased to 341 and 87 days, respectively, in MVI-negative patients (n = 19). Patients achieving partial response (PR) and stable disease (SD) status as well as those achieving SD status showed significantly better survival than patients with progressive disease (PD status): PR + SD vs. PD: MST = 447 vs. 123 days, P < 0.001; SD vs. PD: MST = 447 vs. 123 days, P = 0.001). No serious adverse event or treatment-related death occurred. Conclusions: HAIC with cisplatin can be safely administered in patients with HCC and C-P score ≥ 8, who are TACE-refractory or -ineligible. An extended survival time is expected when the treatment outcome is either SD or more favorable.
A prospective study to determine the application of site, ischemia, neuropathy, bacterial infection and depth scoring in the outcome and management of diabetic foot ulcers

Foot ulcers are common complication of diabetes and represent a major source of morbidity. During the year 2014 the number of cases of diabetes worldwide was estimated to be 422 million with an estimated 1.5 million deaths due to high blood glucose. The diabetic foot has increased from 0.7% in the 1980-88 period to more than 2.7% in the years 1998-2004. Lower limb amputations are at least 10 times more common in diabetics than nondiabetics.
Free vibration of microscale frameworks using modified couple stress and a combination of Rayleigh–Love and Timoshenko theories

A model is proposed for investigating the size-dependent frequency response of arbitrarily oriented microscale frames used in the build-up of lattice structures with micro unit cells. The model employs the Rayleigh–Love, the Timoshenko and the modified couple stress theories to overcome the weaknesses of the conventional theories. Descriptions of the model and finite element implementation are presented. Predictions from the reduced forms of the model agree with published results. The frequency analyses of different microscale frames reveal the influence of material lengthscale, dead weight, lateral inertia and orientation angles. For small aspect ratios, neglecting the lateral inertia effect incurs a substantial error in predicting the frequencies of higher modes, but only marginally affects the lower modes. The resonant frequencies exhibit a sharp drop in the presence of dead weight, but it increases in the presence of material lengthscale.
A COMPARATIVE RANDOMISED CONTROLLED PARALLEL GROUP STUDY OF MATERNAL, FETAL AND NEONATAL OUTCOMES OF LABETALOL VERSUS METHYLDOPA IN THE TREATMENT OF NEW ONSET HYPERTENSION DURING PREGNANCY

Hypertensive disorders in pregnancy remain one of the major causes of maternal and perinatal mortality in developing as well as developed countries and can result in hospital admission, preeclampsia and poss ible premature delivery besides other complications to the fetus and neonate. Antihypertensive drugs are often used to lower blood pressure to prevent this progression to adverse outcomes for the mother and the fetus. Hence, we wanted to compare the maternal, fetal and neonatal outcomes of antihypertensive drugs � labetalol versus methyldopa in pregnancy induced hypertension (PIH) in an Indian population. We carried out a prospective randomised controlled parallel group study on 90 outpatients as well as inpatients of the antenatal ward of Obstetrics and Gynaecology department of our tertiary care teaching hospital. Pregnant patients (20�40 weeks gestational age) newly diagno sed with blood pressure of ≥140/90mmHg and singleton pregnancies with vertex presentation were included in the study. 45 patients each were randomised to either of the two treatment arms - oral methyldopa or oral/IV labetalol. Difference in maternal and fetal/neonatal outcome measurements preand posttreatment (on 8 th day) were analysed by applying paired't' test. For inter group analysis, we applied chisquare test, u sing Epi Info statistical software version 3.3. A Pvalue < 0.05 was regarded as significant with 95% confidence limits. Significantly better maternal outcomes on proteinuria, blood urea, platelet count were seen with labetalol than with methyldopa, but not for rates of spontaneous labour and normal vaginal delivery which were higher with methyldopa. Hypertensive crisis and eclampsia occurred in both groups, without significant difference between groups. There was no significant difference between groups for any of the fetal and neonatal outcomesstillbirth, death in first week , birth weight, preterm delivery, Apgar score, need for in tensive neonatal care, jaundice, hypoglycaemia and neonatal bradycardia, but incidence of RDS was significantly lower in labetalol group. So, labetalol is comparable to methyldopa in treating and preventing adverse maternal, fetal and neonatal outcomes in new onset hypertension during pregnancy.
Evaluation of the Socio-demographic and Psychiatric Features of the Adolescents who were Referred to a Child Psychiatry Clinic for the Forensic Evaluation for Early Marriage

1Marmara Üniversitesi Pendik Eğitim ve Araştırma Hastanesi, Çocuk ve Ergen Ruh Sağlığı ve Hastalıkları Kliniği, İstanbul, Türkiye 2Balıkesir Atatürk Şehir Hastanesi, Çocuk ve Ergen Ruh Sağlığı ve Hastalıkları Kliniği, Balıkesir, Türkiye 3Marmara Üniversitesi Tıp Fakültesi, Çocuk ve Ergen Ruh Sağlığı ve Hastalıkları Anabilim Dalı, İstanbul, Türkiye Onur Tuğçe Poyraz Fındık1, Burcu Gökalp Özcan2, Ayşe Rodopman Arman3, Funda Gümüştaş1 Erken Evlendirilmeleri için Adli Rapor Talebi ile Çocuk Psikiyatri Polikliniğine Yönlendirilen Ergenlerin Sosyodemografik ve Ruhsal Özelliklerinin Değerlendirilmesi
Comparison of language skills of students with and without reading difficulties

The aim of this study was to examine the language skills of students with and without reading difficulties (RD) comparatively. For this purpose, a comparative descriptive research methodology was utilised with a participant group comprised of 52 elementary school 3 rd grade students with and without RD. The results of this study revealed that students with difficulties in reading fluency were less successful in phonological processing than their peers with no difficulty in reading fluency. Also, students who had difficulties in reading comprehension were less successful than their peers with no difficulties in oral language assessments. In summary, it was recognised that students who experienced difficulties in reading also possessed difficulties in language skills. As a result, the assessment of both reading skills and language skills is critical for the identification of RD as well as for conducting appropriate intervention programs to address these difficulties.
A national survey of academic articles reading and retrieving of laboratory professionals

Background: The laboratory medicine is usually an important hub between the patient and health care system in aspects of diagnosis and monitory of diseases. With so much attention drawn toward the provision of quality laboratory services, it becomes imperative to assess the knowledge of laboratory professionals. This work was therefore aimed at understanding the learning and working situation of laboratory professionals through the investigation of article reading and retrieving. Methods: The questionnaire designed by “Questionnaire Star” was launched for a month. The survey was conducted among the national laboratory professionals by WeChat from September 27, 2016 to October 27, 2016. The outcome was retrieved and analyzed by “Questionnaire Star” again. Results: There are 883 questionnaire replies across more than 30 districts in the survey. The responders are the laboratory professionals from different positions including the university students, the postgraduate students, the staffs working at hospital, teachers, retired staffs and so on. When talking about the frequency of literature reading, 59.3% of them chose “monthly or yearly”, 25.8% of them chose “weekly”, 9.1% of the repliers preferred to “daily” and 5.9% never do it. As regards the frequency of consulting academic databases, 37.9% of responders accessed scientific databases weekly and 33.2% accessed monthly, followed were yearly (14.2%), daily (8.1%) and never (6.1%). In addition, we found that 55.4% of the responders never published articles and 40.3% whose article publication numbers were less than 5 every year. Of course, the impact factors of those publications were less than 5 (89.1%) mostly. Conclusions: The articles reading and retrieving situation of laboratory professionals were simply found by this survey. And it will have a positive influence on the improvement of their specialty learning.
Possible Protective Effect of Melatonin on Cisplatin-Induced Testicular Toxicity in Adult Albino Rats. A Histological and Immunohistochemical Study.

ABSTRACT:Introduction: Chemotherapy may result in temporary or permanent gonadal toxicity in male patients. Loss of fertility potential can be devastating to patients, especially, during the child-bearing period. Aim of Work: The present study was planned to investigate the possible protective effect of melatonin, in a rat model of cisplatin-induced testicular toxicity. Materials and Methods: Forty five adult male albino rats (80-200 grams each) were divided into four groups; Group I Control (n=15) received 0.9% sodium chloride and/or distilled water as a vehicle. Group II (n=10) were injected intraperitoneally (I.P.) with a single dose 7 mg/kg of cisplatin& were sacrificed after 10 days. Group III (n=10) received melatonin daily orally at a dose of 8 mg/kg/day for 10 days. Group IV (n=10); oral melatonin administration started 5 days before the single I.P. injection of cisplatin, followed by continuation of melatonin for further 10 days. Testicular sections were subjected to HE confirming the anti-oxidant potential of melatonin. Conclusion: Our ﬁndings provide experimental evidence ensuring the protective effect of melatonin, when administered prior to, and concomitant with the chemotherapeutic agent cisplatin. Meanwhile, melatonin administered alone seemed to induce injury to the testis.Keywords: Cisplatin, rat testis, melatonin, iNOS, AR.Corresponding Author : Khaled Ragab Abd El-Hakim , MD, Histology Department , Faculty of medicine , Misr University for Science and Technology, Cairo, Egypt, Tel: +201223313283, E-mail: khaled_rag@yahoo.com.
Antibiotic use for the Prevention and Treatment of Rheumatic Fever and Rheumatic Heart Disease in Children

Summary_________________________________________________________________________ 3 Introduction ______________________________________________________________________ 3 Background and Disease Burden of Rheumatic Heart Disease _____________________________ 4 Current Guidelines________________________________________________________________ 5 Discussion ________________________________________________________________________ 7 Primordial and Primary Prevention __________________________________________________ 7 Antibiotic Treatment of Acute Rheumatic Fever _________________________________________ 7 Secondary Prophylaxis (Prevention)__________________________________________________ 7 Recent publications supporting this regimen____________________________________________ 9 Unanswered Questions ____________________________________________________________ 9 Conclusion ______________________________________________________________________ 10 References_______________________________________________________________________ 11 Appendix________________________________________________________________________ 14
Composition de la poudre des feuilles sèches de moringa oleifera dans trois régions du Niger

In Niger, the acute and chronic malnutrition constitutes a public health problem among children aged from six to fifty-nine months. The use of local foods was identified in the national protocol of malnutrition management as an alternative for ready to use therapeutic foods that are currently used. Among these local foods, the powder of dried leaves of Moringa oleifera was analyzed in order to know the composition of the powder of dried leaves of Moringa oleifera produced in Niger compared to the available data. To achieve this, four samples from the three main Moringa production regions in Niger namely Tillaberi, Niamey both located in the river basin and southern Maradi of the Sahel-Sudanese zone, were analyzed. The chromatographic analysis hasbeen made in the laboratory of the International Crops Research Institute for the Semi- Arid Tropics. The nitrogen extraction technique was done using H 2 SO 4 digestion + salicylic acid + H 2 O 2 and selenium. It was measured using the calorimetry auto analyzer, based the Bertholet reaction. Total phosphorous, iron, zinc, potassium, calcium, magnesium, sodium and copper was also extracted, using the same method. Phosphorus was measured by calorimetry using the method of molybdo-phosphate reduced to ascorbic acid. The other elements were measured using atomic spectroscopy absorption. The results correspond to the average of two parallel tests. Five standards enabled to make the calibration and a control sample was analyzed in the same conditions. The test is valid if the regression is about 0,9950 - 0,9999. The powder of dried leaves of Moringa produced in Niger turned out be rich in proteins with an average of 24, 8 % (Confidence Interval at 95%: 19, 34-30, 24) with values going from 21,58% in Tillaberi to 28,72% in Niamey and in micronutrients. According to regions, the composition varies for iron between 51,9 and 55,12 mg/100g, 0,45 and 1,58 mg/100g for zinc, 1192,5and 1957,5 mg/100g for calcium, 414,37 and 714,37 mg/100g for magnesium,1587 and 2037 mg/100g for potassium, 207,75 and 326,25 mg/100g for sodium, 32 and 61 mg/100g for phosphorus. These results are close to those revealed by earlier studies. Key words: Moringa oleifera , composition, powder, dry, leaves, different, regions, Niger
Tourist attractiveness: measuring residents’ perception of tourists

Abstract To date, tourism research has primarily explored resident perception and attitude in terms of tourism impact and development. Comparatively, resident perception of tourists themselves has attracted less attention from tourism researchers despite the fact host communities see the benefits of tourism through positive relationships with tourists. Moreover, studies on the resident-tourist relationship have largely been interested in the perspective of tourists, considering tourists as customers who bring economic benefits to a destination, while seeing residents as beneficiaries of the tourists’ expenditures during their trip. The skewed approach has amplified the incomplete understanding of the resident-tourist relationship, disregarding the importance of reciprocity and the merchandized purpose of the exchanges in this relationship. As a starting point to study the residents' perspectives toward the resident-tourist relationship, this study explored and operated the concept of tourist attractiveness through a mixed-methods approach using a case study of Jeju Island, South Korea. As a result, this study found that tourist attractiveness can be measured using five sub-dimensions: tourist compatibility, tourist financial capacity, tourist responsibility, positive tourist influence, and negative tourist influence.