Miracle-dz/UniFAR-FTUs huggingface dataset:
Monitoring the tacrolimus concentration in peripheral blood mononuclear cells of kidney transplant recipients

Tacrolimus is a critical dose drug and to avoid under‐ and overexposure, therapeutic drug monitoring is standard practice. However, rejection and drug‐related toxicity occur despite whole‐blood tacrolimus pre‐dose concentrations ([Tac]blood) being on target. Monitoring tacrolimus concentrations at the target site (within peripheral blood mononuclear cells; [Tac]cells) may better correlate with drug‐efficacy. The aim of this study was to (1) investigate the relationship between [Tac]blood and [Tac]cells, (2) identify factors affecting the tacrolimus distribution in cells and whole‐blood, and (3) study the relationship between [Tac]cells and clinical outcomes after kidney transplantation.
Monitoring the sentiment of cannabis-related tweets in the lead up to New Zealand's cannabis referendum.

INTRODUCTION AND AIMS In October 2020, New Zealanders will vote on whether cannabis should be legalised for recreational use. With this in mind, the aim of the present study is to gauge the views and opinions of the New Zealand population on cannabis via tweets. To achieve this, we conducted a sentiment analysis of all historic cannabis-related tweets and referendum-specific tweets written in New Zealand. DESIGN AND METHODS We used a Twitter-sponsored commercial platform to access all historic cannabis-related tweets written in New Zealand and used search terms to remove non-cannabis-related terms. Next, we used the platform's machine learning function to code the sentiment of tweets (i.e. positive/pro-cannabis, negative/anti-cannabis or neutral). RESULTS Between July 2009 and August 2020, 304 760 cannabis-related tweets were written in New Zealand. Overall, the tweets were predominantly positive (62.0%) and there was a higher proportion of positive tweets written in 2020 (65.3%) compared to negative or neutral tweets. Similarly, for referendum-specific tweets, the 2020 data reveal a generally positive view of cannabis (53.5%). DISCUSSION AND CONCLUSIONS Both cannabis-related, and referendum-specific tweets, suggest that Twitter users in New Zealand have a generally positive view of cannabis. Given the nature of Twitter, the current method will allow us to study whether views toward cannabis change as the referendum nears and capture any late swings in pro- or anti-cannabis sentiment (abcd-lab.shinyapps.io/cannabis_sentiment/).
Susceptibility Factors in Chronic Lung Inflammatory Responses to Engineered Nanomaterials

Engineered nanomaterials (ENMs) are products of the emerging nanotechnology industry and many different types of ENMs have been shown to cause chronic inflammation in the lungs of rodents after inhalation exposure, suggesting a risk to human health. Due to the increasing demand and use of ENMs in a variety of products, a careful evaluation of the risks to human health is urgently needed. An assessment of the immunotoxicity of ENMs should consider susceptibility factors including sex, pre-existing diseases, deficiency of specific genes encoding proteins involved in the innate or adaptive immune response, and co-exposures to other chemicals. This review will address evidence from experimental animal models that highlights some important issues of susceptibility to chronic lung inflammation and systemic immune dysfunction after pulmonary exposure to ENMs.
Home Range and Movements of the Bog Turtle (Clemmys muhlenbergii) in Maryland

We studied the home ranges and movements of 50 bog turtles (Clemmys muhlenbergii) at two sites in Harford County, Maryland, from April 1996 to August 1997. These sites differ in size, land use, and stage of vegetative succession. One site (HA406) was intensively studied 17 years ago. Using radiotelemetry, we located turtles two times a week during the active season. The 95% Adaptive Kernel home range esti- mator was used to determine home ranges. Individual turtle home ranges varied from 0.003 ha to 3.12 ha with considerable variation between sites and years. There were no significant differences in home range size or movements between sexes or seasons (mating vs. postmating), although males had larger home ranges during the mating season. Many turtles at site HA406 had home ranges far larger than the average home range reported in the earlier study. An expansion in turtle home ranges may suggest a decrease in site quality resulting from invasion of multiflora rose. Some turtles moved out of their wetlands and across barriers, a finding that demonstrates the importance of appropriate habitat in corridors that connect popu- lations.
Function of the SNARE Ykt6 on autophagosomes requires the Dsl1 complex and the Atg1 kinase complex

The mechanism and regulation of fusion between autophagosomes and lysosomes/vacuoles are still only partially understood in both yeast and mammals. In yeast, this fusion step requires SNARE proteins, the homotypic vacuole fusion and protein sorting (HOPS) tethering complex, the RAB7 GTPase Ypt7, and its guanine nucleotide exchange factor (GEF) Mon1‐Ccz1. We and others recently identified Ykt6 as the autophagosomal SNARE protein. However, it has not been resolved when and how lipid‐anchored Ykt6 is recruited onto autophagosomes. Here, we show that Ykt6 is recruited at an early stage of the formation of these carriers through a mechanism that depends on endoplasmic reticulum (ER)‐resident Dsl1 complex and COPII‐coated vesicles. Importantly, Ykt6 activity on autophagosomes is regulated by the Atg1 kinase complex, which inhibits Ykt6 through direct phosphorylation. Thus, our findings indicate that the Ykt6 pool on autophagosomal membranes is kept inactive by Atg1 phosphorylation, and once an autophagosome is ready to fuse with vacuole, Ykt6 dephosphorylation allows its engagement in the fusion event.
Identification of Core Genes Involved in the Progression of Cervical Cancer Using an Integrative mRNA Analysis

In spite of being a preventable disease, cervical cancer (CC) remains at high incidence, and it has a significant mortality rate. Although hijacking of the host cellular pathway is fundamental for developing a better understanding of the human papillomavirus (HPV) pathogenesis, a major obstacle is identifying the central molecular targets involved in HPV-driven CC. The aim of this study is to investigate transcriptomic patterns of HPV-infected and normal tissues to identify novel prognostic markers. Analyses of functional enrichment and interaction networks reveal that altered genes are mainly involved in cell cycle, DNA damage, and regulated cell-to-cell signaling. Analysis of The Cancer Genome Atlas (TCGA) data has suggested that patients with unfavorable prognostics are more likely to have DNA repair defects attributed, in most cases, to the presence of HPV. However, further studies are needed to fully unravel the molecular mechanisms of such genes involved in CC.
Bioavailability of Organic Matter in the Superficial Sediment of Guanabara Bay, Rio de Janeiro, Brazil Biodisponibilidade da Matéria Orgânica dos Sedimentos Superficiais da Baía de Guanabara, Rio de Janeiro, Brasil

Thirty superficial sediment samples were collected in Guanabara Bay in order to identify new trophic state and environmental quality descriptors for coastal systems. A biochemical approach was used for analyzing the quality and quantity of sedimentary organic matter and metabolic bacterial activity. The samples were analyzed for particle size; organic matter, protein, carbohydrate, lipid, biopolymeric carbon, and bioavailable carbon levels; and bacterial metabolic activity. The results show a homogeneous spatial distribution for the anaerobic bacteria web and for biopolymers (carbohydrates>lipids>protein). The NE area of the bay displayed sediment lipid levels above 1 mg/g, indicative of organic sewage input. Spatial distribution of the superficial sediments in relation to other variables was not significant (p>0.05). Biopolymers and labile organic matter showed a significant correlation with the average particle size of 80% of the fine particles. Despite the availability of labile organic matter, under the form of biopolymeric carbon, only 50% of the carbon was available to the trophic web. The bacterial consortia formed by sulfate reducing and denitrifying bacteria sustain the benthic trophic food web in Guanabara Bay.
Impacts of Dynamic LED Lighting on the Well-Being and Experience of Office Occupants

As a critical factor in the built environment, lighting presents considerable influence on occupants. Previous research across static lighting conditions has found that both illuminance and correlated color temperature (CCT) affect occupants’ physiological and psychological functioning. However, little research has been conducted on the non-visual impacts of dynamic lighting with daily variation in illuminance and CCT levels. The purpose of this study is to better understand the impact of dynamic lighting on office occupants’ health, well-being and experience at a living lab. Fifteen participants were recruited to work in three office modules for four months. Four lighting conditions were designed and implemented in this study, including two static lighting conditions and two dynamic lighting conditions with a specific predefined control scheme. A prototype lighting system with enhanced control capabilities was configured and implemented to ensure the desired lighting environment protocol. Both objective methods and subjective surveys were used to assess the behavioral and physiological outcomes of interest, including mental stress, sleep, productivity, satisfaction, mood, visual comfort and perceived naturalness. The results showed that the daytime behavioral impacts were either positive or mixed. Specifically, a significant alertness increase was observed in the afternoon, indicating a potential solution to reduce the natural feelings of sleepiness during the workday. There was also a marginal benefit for mood. The nighttime impacts include a significant decrease in perceived sleep quality and sleep time after subjects were exposed to dynamic lighting. No significant differences were observed for mental stress, productivity, visual comfort, or perceived naturalness. The findings present additional insights into the non-visual impacts of dynamic lighting and give recommendations for further investigations.
Plasma Macrophage Inhibitory Cytokine-1 as a Complement of Epstein-Barr Virus Related Markers in Identifying Nasopharyngeal Carcinoma

Background: We evaluated the diagnostic value of plasma Macrophage inhibitory cytokine-1 (MIC-1) in distinguishing patients with nasopharyngeal carcinoma (NPC) and explored its complementary role with widely used Epstein-Barr virus (EBV) related markers, EBV capsid antigen-specific IgA (VCA-IgA) and EBV copy number. Methods: ELISA was used to analyze the plasma MIC-1 levels in 190 NPC patients, 72 VCA-IgA-positive healthy donors (VP), and 219 normal subjects with negative VCA-IgA (VN). 10 pairs of plasma samples before and after radiotherapy were also included. Results: The plasma MIC-1 levels were significantly higher in NPC patients (Median: 678.39 ng/mL) than those in VN and VP (310.29 and 294.59, p < 0.001). Receiver operating characteristic (ROC) curves of the MIC-1 concentrations revealed that the area under the ROC curve (AUC) was 0.790 (95% confidence interval [CI]: 0.748-0.832), with a sensitivity of 63.7%, and a specificity of 85.9% respectively, for distinguishing NPC patients from the healthy donors. Similarly, between NPC and VP, ROC was 0.796 (0.738-0.853) with sensitivity of 63.7%, and specificity of 88.9%. In addition, between NPC and VN, ROC was 0.788(0.744-0.832) with sensitivity of 63.7%, and specificity of 84.9%. Further, we found that MIC-1 could complement VCA-IgA and EBV DNA markers, with a negative rate of 88.9% in VCA-IgA-positive healthy controls, and a positive rate of 59.0% in EBV DNA negative NPC patients, respectively. Also, the MIC-1 plasma concentration dropped significantly after radiotherapy (p = 0.027). Conclusions: MIC-1 can complement VCA-IgA titers and EBV DNA copy number tests in NPC detection, improve identification of EBV DNA-negative NPC patients, and distinguish NPC from VCA -IgA positive healthy controls.
Slope of Kidney Function and Its Association with Longitudinal Mortality and Cardiovascular Disease among Individuals with CKD.

BACKGROUND Slopes of eGFR have been associated with increased risks of death and cardiovascular events in a U-shaped fashion. Poor outcomes in individuals with rising eGFR are potentially attributable to sarcopenia, hemodilution, and other indicators of clinical deterioration. METHODS To investigate the association between eGFR slopes and risks of death or cardiovascular events, accounting for multiple confounders, we studied 2738 individuals with moderate to severe CKD participating in the multicenter Chronic Renal Insufficiency Cohort (CRIC) Study. We used linear, mixed-effects models to estimate slopes with up to four annual eGFR assessments, and Cox proportional hazards models to investigate the association between slopes and the risks of death and cardiovascular events. RESULTS Slopes of eGFR had a bell-shaped distribution (mean [SD], -1.5 [-2] ml/min per 1.73 m2 per year). Declines of eGFR that were steeper than the average decline associated with progressively increasing risks of death (hazard ratio [HR], 1.23; 95% confidence interval [95% CI], 1.09 to 1.39; for a slope 1 SD below the average) and cardiovascular events (HR, 1.19; 95% CI, 1.03 to 1.38). Rises of eGFR or declines lower than the average decline were not associated with the risk of death or cardiovascular events. CONCLUSIONS In a cohort of individuals with moderate to severe CKD, we observed steep declines of eGFR were associated with progressively increasing risks of death and cardiovascular events; however, we found no increased risks associated with eGFR improvement. These findings support the potential value of eGFR slopes in clinical assessment of adults with CKD.
Effect of some fungicides on mycelium growth of Fusarium avenaceum (Fr.) Sacc. pathogenic to chrysanthemum (Dendranthema grandiflora Tzvelev)

Ten fungicides were tested in vitro for their effectiveness to inhibit the linear growth of three isolates of Fusarium avenaceum of proven pathogenicity to chrysanthemum. The measurements were taken after 4 and 8 days of fungus growth in the presence of fungicides in three concentrations. The most effective in vitro proved to be the fungicides containing difenaconazole, carbendazim and flusilazol while the least effective were mancozeb, chlorothalonil and captan. The isolates of F. avenaceum differed in their susceptibility to tested fungicides.
The Evolution of Complex Es Observed by Multi Instruments Over Low‐Latitude China

A complex daytime sporadic E (Es) case with extremely high critical frequency (foEs) was observed over the low latitude of China on 19 May 2018. Simultaneous observational results from two very high frequency (VHF) radars, two ionosondes, and multiple Global Navigation Satellite System total electron content and scintillation receivers are analyzed to investigate the evolution of the complex Es occurrence, which consisted of a relatively weak ambient Es layer (foEs < 8 MHz) and band‐like strong Es structures (foEs > 17 MHz) drifting from higher latitude. The strong Es structures elongated more than 500 km in the northwest‐southeast direction, drifted southwestward at a speed of ~65 m/s. VHF radar backscatter echoes were generated when the strong Es structures passed the radar field of view, with different echo patterns due to different radar and antenna configurations. No VHF radar backscatter echo was associated with the ambient Es layer. The mechanisms responsible for the formations of the ambient Es layer and band‐like strong Es structures are addressed and discussed.
The Effect of Smartphones Use on Altering the Level of Attention and Learning Styles among Undergraduate Nursing Students in Kenya

Background: The use of cell phones can harm nursing students' academic performance. As such, reliance on the device for everyday activities has increased. Consequently, "nomophobia", defined as the fear of being without one's smartphone, has emerged as a new phobia among the users. Aim: This study was aimed at identifying the effects that long-term use of smartphone has among students cause on their level of attention and learning styles. Methodology: A quasi-experimental study was carried out among nursing students in two public universities located in western Kenya. The sample size comprised of 811 thirdand fourth-years nursing students with a control group (n = 349, 43. %) and an experimental group (n = 462, 57%). The experimental group left their cell phones, in sound or vibration mode, as they normally kept them, on a table located in the corner of the classroom. Data were analysed using SPSS v.25, and data presented using graphs and tables. The data was reliable at alpha . 824 and without outliers. Results: More female 54.87% than male was evidenced by the responses. Both groups showed higher-than-average levels of nomophobia at the beginning of the class. As for problematic smartphone use and attention, it was found that there were statistically significant differences in both cases (U = 1355.500, Z = −2.830, P = .005; U = 1449.000, Z = −2.363, P = .018, respectively), and the experimental group had higher average scores, as well as in those related to knowledge. Testing of the hypothesis was done and the null hypothesis was rejected (X2=19.609, P>0.001). Conclusion: A relationship has been found between nomophobia and problematic smartphone use. Students who do not have access to their cell phones during class perceive more problematic cell phone usage, in addition to paying closer attention during class. Recommendation: Because of this, it is important to an alternative way of reducing the nomophobia in order to enhance attention and learning styles among students. Future studies are recommended on lecturers not only the students the medical students but also in other departments of the university and expand to other universities across the country.
Expression of ACE2, the SARS-CoV-2 Receptor, in Lung Tissue of Patients With Type 2 Diabetes

Increased expression of pulmonary ACE2, the SARS-CoV-2 receptor, could contribute to increased infectivity of COVID-19 in patients with diabetes, but ACE2 expression has not been studied in lung tissue of subjects with diabetes. We therefore studied ACE2 mRNA and protein expression in lung tissue samples of subjects with and without diabetes that were collected between 2002 and 2020 from patients undergoing lobectomy for lung tumors. For RT-PCR analyses, samples from 15 subjects with diabetes were compared with 91 randomly chosen control samples. For immunohistochemical staining, samples from 26 subjects with diabetes were compared with 66 randomly chosen control samples. mRNA expression of ACE2 was measured by quantitative RT-PCR. Protein levels of ACE2 were visualized by immunohistochemistry on paraffin-embedded lung tissue samples and quantified in alveolar and bronchial epithelium. Pulmonary ACE2 mRNA expression was not different between subjects with or without diabetes. In contrast, protein levels of ACE2 were significantly increased in both alveolar tissue and bronchial epithelium of patients with diabetes compared with control subjects, independent of smoking, chronic obstructive pulmonary disease, BMI, renin-angiotensin-aldosterone system inhibitor use, and other potential confounders. To conclude, we show increased bronchial and alveolar ACE2 protein expression in patients with diabetes. Further research is needed to elucidate whether upregulation of ACE2 expression in airways and lungs has consequences on infectivity and clinical outcomes of COVID-19.
Absence of post-learning motor activity effects on memory for motor-related words

ABSTRACT Drawing a hypothesis from embodied theories of memory, van Dam, Rueschemeyer, Bekkering and Lindemann [(2013). Embodied grounding of memory: Toward the effects of motor execution on memory consolidation. The Quarterly Journal of Experimental Psychology, 66(12), 2310–2328] showed that recognition performance for action words could be modulated by actions performed during the retention interval, suggesting that motor actions during the retention interval affect memory consolidation. The results of 4 experiments from two different laboratories, designed to replicate and extend the van Dam et al. motor consolidation effect, are presented here. Two of the experiments (n = 30 and n = 44) exactly and independently replicated the experimental design and conditions of the original experiment. Yes/No recognition scores plus additional analysis of response times showed no motor consolidation effects. A third experiment (n = 44) manipulating type of processing during encoding also failed to find significant motor consolidation effects. Finally, a fourth experiment (n = 120) following a more standard reconsolidation paradigm, involving 24-hour intervals between learning and motor behaviour, and a 24-hour delayed test, also found null effects. The absence of effects of motor execution on memory consolidation is discussed in terms of the implications of these findings for the embodiment approach to cognition.
Comparing Evolutionary Algorithms for Deep Neural Networks

Genetic algorithms have long been successfully applied to optimize the weights of neural networks. The ge-netic concept of crossover, recombining components to form a stronger component, applies well to Deep Neural Networks. This paper elaborates on the performances of two automated evolutionary methods for optimizing deep learning architectures on the relevant and important tasks of image classiﬁcation and language modeling. The ﬁrst of these methods is based on evolving populations of chromosomes and crossing over the strongest amongst them, and the second is based on a hierarchical genetic representation scheme. Both of the algorithms required a substantial amount of computational power in the original literatures. Given the limited resources available, presented are the implementations of these algorithms at a relatively small scale for both tasks as well as results on the CIFAR-10 dataset, demonstrating classiﬁcation quality comparable to that of literature and setting the stage for the future work of merging these methodologies.
The Oxford Dodo. Part 2: from curiosity to icon and its role in displays, education and research

Abstract The Tradescant or Oxford Dodo has played an important role in exhibition and education throughout its 360-year history, and has been the subject of scientific research reflecting changing interests and techniques over this time. Due to confusion over its relationships, its placement in the classification systems continually changed, until the dissection in 1847 of the head and foot confirmed its columbid (pigeons and doves) affinities. Here, we describe the dissection of the head and foot and the Tradescant Dodo’s display history, from the late nineteenth century until the present day, and also its use in education. We discuss the importance of the Tradescant Dodo to Lewis Carroll, whose ‘Alice’s Adventures in Wonderland’ made the dodo aware to a worldwide audience. We further provide an overview of recent work including electron microscopic study of feathers, mDNA analysis, cytological investigation of the skin and measuring of the brain capacity, all of which have added to our knowledge of the evolution and ecology of this most extraordinary of birds. Research is ongoing; the Tradescant Dodo is presently subject to CT scanning and functional analysis. This is the second part of a two-part paper.