Miracle-dz/UniFAR-FTUs huggingface dataset:
Evidence for high-density liquid water between 0.1 and 0.3 GPa near 150 K

Significance The two-liquid model is appealing to explain the anomalous nature of supercooled water. However, it is still contested whether or not the amorphous polymorphism at cryoconditions develops into a liquid–liquid transition at higher temperatures. While some work has been devoted to this question at ambient pressure conditions, there are barely any experiments at high-pressure conditions. Here we reveal a temperature window, in which bulk high-density liquid water (HDL) can be accessed prior to crystallization at 0.1–0.3 GPa based on path independence. By contrast, at higher pressures crystallization takes place from the amorphous solid state. This paves the way for future exploration of the liquid–liquid transition from HDL to low-density liquid water at thermodynamic equilibrium near 0.1 GPa. Thermal stability against crystallization upon isobaric heating at pressure 0.1 ≤ P ≤ 1.9 GPa is compared for five variants of high- (HDA) and very high-density amorphous ice (VHDA) with different preparation history. At 0.1–0.3 GPa expanded HDA (eHDA) and VHDA reach the same state before crystallization, which we infer to be the contested high-density liquid (HDL). Thus, 0.3 GPa sets the high-pressure limit for the possibility to observe HDL for timescales of minutes, hours, and longer. At P > 0.3 GPa the annealed amorphous ices no longer reach the same state before crystallization. Further examination of the results demonstrates that crystallization times are significantly affected both by the density of the amorphous matrix at the crystallization temperature Tx as well as by nanocrystalline domains remaining in unannealed HDA (uHDA) as a consequence of incomplete pressure-induced amorphization.
Impairment of Intestinal Monocarboxylate Transporter 6 Function and Expression in Diabetic Rats Induced by Combination of High-Fat Diet and Low Dose of Streptozocin: Involvement of Butyrate–Peroxisome Proliferator-Activated Receptor-γ Activation

Generally, diabetes remarkably alters the expression and function of intestinal drug transporters. Nateglinide and bumetanide are substrates of monocarboxylate transporter 6 (MCT6). We investigated whether diabetes down-regulated the function and expression of intestinal MCT6 and the possible mechanism in diabetic rats induced by a combination of high-fat diet and low-dose streptozocin. Our results indicated that diabetes significantly decreased the oral plasma exposure of nateglinide. The plasma peak concentration and area under curve in diabetic rats were 16.9% and 28.2% of control rats, respectively. Diabetes significantly decreased the protein and mRNA expressions of intestinal MCT6 and oligopeptide transporter 1 (PEPT1) but up-regulated peroxisome proliferator-activated receptor γ (PPARγ) protein level. Single-pass intestinal perfusion demonstrated that diabetes prominently decreased the absorption of nateglinide and bumetanide. The MCT6 inhibitor bumetanide, but not PEPT1 inhibitor glycylsarcosine, significantly inhibited intestinal absorption of nateglinide in rats. Coadministration with bumetanide remarkably decreased the oral plasma exposure of nateglinide in rats. High concentrations of butyrate were detected in the intestine of diabetic rats. In Caco-2 cells (a human colorectal adenocarcinoma cell line), bumetanide and MCT6 knockdown remarkably inhibited the uptake of nateglinide. Butyrate down-regulated the function and expression of MCT6 in a concentration-dependent manner but increased PPARγ expression. The decreased expressions of MCT6 by PPARγ agonist troglitazone or butyrate were reversed by both PPARγ knockdown and PPARγ antagonist 2-chloro-5-nitro-N-phenylbenzamide (GW9662). Four weeks of butyrate treatment significantly decreased the oral plasma concentrations of nateglinide in rats, accompanied by significantly higher intestinal PPARγ and lower MCT6 protein levels. In conclusion, diabetes impaired the expression and function of intestinal MCT6 partly via butyrate-mediated PPARγ activation, decreasing the oral plasma exposure of nateglinide.
Femoral and tibial alignments in chihuahuas with patellar luxation by radiograph: Angular values and intra- and inter-observer agreement of measurements

Measurement of fore and pelvic limb alignment in veterinary orthopedics is significant, as it is in human medicine. The establishment of reference ranges for alignments and comparing measured ranges in diseased animals to these reference values would allow veterinarians to specify the quantitative degree of an angular deformity, plan suitable treatments and evaluate treatment outcomes. Patellar luxation is a common orthopedic disease in small animal clinics. Severe grades of MPL may present with bone deformities or abnormal alignments; therefore, evaluation of the measurement methods of femoral and tibial alignment in dogs with different grades of patellar luxation to assess the accuracy and reliability of the measurements could be useful. In this retrospective study radiographs of 21 client-owned Chihuahuas that had been presented to the Small Animal Surgery of Vetmeduni Vienna from 2012–2016 with a diagnose of patellar luxation were selected. The measurements were performed on frontal, lateral and axial view radiographs to determine the femoral and tibial angles and to evaluate the intra- and inter-observer variabilities of the protocol. Radiographs of each dog were investigated by three observers. Intra-observer variability was based on measurements by each observer who repeated the protocol two times to evaluate repeatability. Inter-observer variability was based on the measurements between the three observers to evaluate the reproducibility of the protocol. The results of the study showed that 92.85% of inter-observer ICC (intra-class correlation coefficient) had high correlation, and the remaining 7.15% had good correlation. Intra-observer ICCs for measurements of the first observer were 28.57% high correlation and 50% good correlation. For the second observer, 100% high correlation was recorded, and for the third observer 71.42% high correlation and 14.28% good correlation was recorded. These results show that the selected methods have high correlation and could be used as a reliable method in veterinary orthopedics.
TriPepSVM: de novo prediction of RNA-binding proteins based on short amino acid motifs

Abstract In recent years, hundreds of novel RNA-binding proteins (RBPs) have been identified, leading to the discovery of novel RNA-binding domains. Furthermore, unstructured or disordered low-complexity regions of RBPs have been identified to play an important role in interactions with nucleic acids. However, these advances in understanding RBPs are limited mainly to eukaryotic species and we only have limited tools to faithfully predict RNA-binders in bacteria. Here, we describe a support vector machine-based method, called TriPepSVM, for the prediction of RNA-binding proteins. TriPepSVM applies string kernels to directly handle protein sequences using tri-peptide frequencies. Testing the method in human and bacteria, we find that several RBP-enriched tri-peptides occur more often in structurally disordered regions of RBPs. TriPepSVM outperforms existing applications, which consider classical structural features of RNA-binding or homology, in the task of RBP prediction in both human and bacteria. Finally, we predict 66 novel RBPs in Salmonella Typhimurium and validate the bacterial proteins ClpX, DnaJ and UbiG to associate with RNA in vivo.
Increased adherence to treatment guidelines in patients with urinary tract infection in primary care: A retrospective study

Background Urinary tract infection (UTI) is common in primary care and leads to a high number of antibiotic prescriptions. Antimicrobial resistance is a global health problem; better antimicrobial prescribing is one way to limit antimicrobial resistance. We aimed to describe the number of consultations for patients diagnosed with lower urinary tract infection (LUTI) and pyelonephritis and changes in prescribing of antibiotics to men and women with LUTI and pyelonephritis in Swedish PHC between the years 2008 and 2013. Methods We performed a descriptive study of changes in UTI diagnosis and antibiotic prescribing in UTI for the years 2008, 2010 and 2013. The Primary Care Record of Infections in Sweden, a database regarding diagnosis linked antibiotic prescribing in primary care, was analyzed concerning data for men and women of all ages regarding UTI visits and antibiotic prescribing. The results were analyzed in relation to current national guidelines. Results There was a variability in consultation incidence for LUTI with an increase between 2008 and 2010 and a decrease between 2010 and 2013, resulting in a slight rise in consultation incidence between 2008 and 2013. The use of recommended nitrofurantoin or pivmecillinam in LUTI in women increased from 54% in 2008 to 69% in 2013. Fluoroquinolones or trimethoprim were prescribed in 24% of LUTI cases in women in 2008 and in 7% of cases in 2013. Prescriptions of pivmecillinam or nitrofurantoin in male LUTI cases increased from 13% in 2008 to 31% in 2013. Fluoroquinolones or trimethoprim were prescribed in 54% of male LUTI cases in 2008 and 32% in 2013. Conclusions Swedish GPs seem to follow national guidelines in the treatment of LUTI in women. In male LUTI cases, the prescriptions of fluoroquinolones remain high and further research is needed to follow prescription patterns and enhance more prudent prescribing to this group of patients.
Laryngology Clinic: Solitary Tracheal Papilloma

A 60-year-old male was transferred to University of Illinois Health Services for further evaluation and management of stridor and dyspnea. He had been followed by his primary care provider and pulmonologist, but deteriorated clinically. He smoked 5 cigars annually and denied any illicit drug use, alcohol use, or occupational exposure to inhaled carcinogens. He had no history of asthma, allergies, or respiratory disorders, but he had been treated for gastroesophagela reflux disease and hypertension. Physical examination was notable for fixed, expiratory, nonpositional stridor, and mild respiratory distress. Computerized tomography with contrast of the neck showed a contrastenhancing, midline 2.7 2.1 3.5 cm endotracheal soft tissue mass causing greater than 90% obstruction of the tracheal lumen. It did not span to adjacent structures. No associated lymphadenopathy was noted. Surgical management began with an urgent tracheotomy and pan-endoscopy. The tracheotomy was performed low to bypass the mass. Biopsy specimens were collected after rigid bronchoscopy confirmed an irregularly shaped, papillomatous mass emanating from the posterior tracheal wall, and extending from the first tracheal ring superiorly to the third tracheal ring inferiorly (see Figure 1). These characteristics and the location of the mass raised suspicions for an aggressive lesion. So, we opted not to attempt complete removal during the initial case. Biopsies were read as squamous papillomas with high-grade squamous dysplasia, but negative for invasive carcinoma. A subtype screen was reported as low risk, 6, and 11. One specimen did reveal squamous cell carcinoma in situ. We returned to the operating room for a complete resection of the mass. Pathology reports remained negative for invasive carcinoma (see Figure 2). In the absence of a pathology specimen
Exploring the mechanism of crashes with automated vehicles using statistical modeling approaches

Autonomous Vehicles (AV) technology is emerging. Field tests on public roads have been on going in several states in the US as well as in Europe and Asia. During the US public road tests, crashes with AV involved happened, which becomes a concern to the public. Most previous studies on AV safety relied heavily on assessing drivers’ performance and behaviors in a simulation environment and developing automated driving system performance in a closed field environment. However, contributing factors and the mechanism of AV-related crashes have not been comprehensively and quantitatively investigated due to the lack of field AV crash data. By harnessing California’s Report of Traffic Collision Involving an Autonomous Vehicle Database, which includes the AV crash data from 2014 to 2018, this paper investigates by far the most current and complete AV crash database in the US using statistical modeling approaches that involve both ordinal logistic regression and CART classification tree. The quantitative analysis based on ordinal logistic regression and CART models has successfully explored the mechanism of AV-related crash, via both perspectives of crash severity and collision types. Particularly, the CART model reveals and visualize the hierarchical structure of the AV crash mechanism with knowledge of how these traffic, roadway, and environmental contributing factors can lead to crashes of various serveries and collision types. Statistical analysis results indicate that crash severity significantly increases if the AV is responsible for the crash. The highway is identified as the location where severe injuries are likely to happen. AV collision types are affected by whether the vehicle is on automated driving mode, whether the crashes involve pedestrians/cyclists, as well as the roadway environment. The method used in this research provides a proven approach to statistically analyze and understand AV safety issues. And this benefit is potential be even enhanced with an increasing sample size of AV-related crashes records in the future. The comprehensive knowledge obtained ultimately facilitates assessing and improving safety performance of automated vehicles.
Universidad, competencias cívico-sociales y mercado de trabajo

En general, la universidad actual tiene asumida como propia la mision de ofrecer una formacion integral que contribuya al desarrollo academico, pero tambien al profesional y social. Asi, cada vez es mayor la importancia otorgada a las competencias civico-sociales, dada su potencial contribucion a la hora de optimizar la transicion del alumnado universitario al mercado de trabajo. El objetivo del presente articulo es estudiar las propiedades psicometricas de una escala para evaluar competencias civico-sociales en la educacion superior y analizar si el ciclo y el area de conocimiento determinan su desarrollo en los estudiantes. Para ello, despues de subdividir aleatoriamente a la muestra (996 alumnos universitarios), se ha procedido a realizar un analisis factorial exploratorio y, a continuacion, confirmatorio. Posteriormente, se ha llevado a cabo un analisis multivariado de varianza 2 × 2 (ciclo × area de conocimiento), tomando como variables dependientes los factores previamente obtenidos. Ademas de una solucion satisfactoria de la escala, tanto en la estructura factorial como en los niveles de consistencia interna, los resultados obtenidos revelan diferencias en torno a las variables objeto de estudio. Mientras que, en general, los alumnos de primer ciclo muestran un alto nivel de desarrollo de estas competencias, no se constatan diferencias en cuanto al area de conocimiento. No obstante, en la interaccion de estas dos variables se evidencian efectos significativos, reflejando puntuaciones mas elevadas en el alumnado de primer ciclo de ciencias de la salud e ingenieria-arquitectura, y en el de segundo ciclo de ciencias sociales-juridicas y artes- humanidades. Se concluye enfatizando la necesidad de que las instituciones de educacion superior promuevan, en mayor medida, el desarrollo de competencias civico-sociales con estrategias metodologicas y programas ajustados a esta exigencia, orientados a la mejora de los niveles de participacion social y la transicion al empleo.
Effects of energy retrofits on Indoor Air Quality in multifamily buildings.

We assessed 45 multifamily buildings (240 apartments) from Finland and 20 (96 apartments) from Lithuania, out of which 37 buildings in Finland and 15 buildings in Lithuania underwent energy retrofits. Building characteristics, retrofit activities, and energy consumption data were collected, and Indoor Air Quality (IAQ) parameters, including carbon monoxide (CO), nitrogen dioxide (NO2 ), formaldehyde (CH2 O), selected volatile organic compounds (benzene, toluene, ethylbenzene, and xylenes (BTEX), radon, and microbial content in settled dust were measured before and after the retrofits. After the retrofits, heating energy consumption decreased by an average of 24% and 49% in Finnish and Lithuanian buildings, respectively. After the retrofits of Finnish buildings, there was a significant increase in BTEX concentrations (estimated mean increase of 2.5 µg/m3 ), whereas significant reductions were seen in fungal (0.6-log reduction in cells/m2 /d) and bacterial (0.6-log reduction in gram-positive and 0.9-log reduction in gram-negative bacterial cells/m2 /d) concentrations. In Lithuanian buildings, radon concentrations were significantly increased (estimated mean increase of 13.8 Bq/m3 ) after the retrofits. Mechanical ventilation was associated with significantly lower CH2 O concentrations in Finnish buildings. The results and recommendations presented in this paper can inform building retrofit studies and other programs and policies aimed to improve indoor environment and health.
Histological analysis of biocompatibility of different surgical adhesives in subcutaneous tissue

The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of cyanoacrylate‐based adhesives in rat subcutaneous tissues. In total, 60 male Wistar rats were used, and divided into four groups (n = 15): Group C (control, PVA‐polyvinyl alcohol sponge), Group NO (N‐butyl‐2‐octylcyanoacrylate), Group NH (n‐hexyl‐cyanoacrylate), and Group EC (Ethyl‐cyanoacrylate). The animals were sacrificed after time intervals of 7, 15, and 30 days and tissues were analyzed under optical microscope as regards the events of inflammatory infiltrate, edema, necrosis, granulation tissue, giant cells, young fibroblasts, and collagen formation. The results were statistically analyzed by the Kruskal–Wallis and Dunn tests (p < .05). Significant inflammatory infiltrate was shown for all the adhesives in the time intervals of 7 (p = .004) and 15 days (p = .003). In the time interval of 30 days, moderate inflammatory infiltrate was observed in Groups NH and EC, with significant difference from Control (p = .001). The quantity of collagen fibers in all the experimental groups showed significant difference compared with Control in the time intervals of 7 (p = .002) and 15 days (p = .001), at 30 days only Group EC showed a smaller quantity of collagen fibers in comparison with Control (p = .001). The hypothesis was rejected. The adhesive N‐butyl‐2‐octylcyanoacrylate had less influence on the inflammatory intensity of multinucleated giant cells. Ethyl‐cyanoacrylate demonstrated the lowest level of biocompatibility among the adhesives, but its use in clinical practice may be promising for coaptation of smaller edges of superficial tissue.
High-dimensional single-cell cartography reveals novel skeletal muscle resident cell populations

Adult tissue repair and regeneration require the activation of resident stem/progenitor cells that can self-renew and generate differentiated progeny. The regenerative capacity of skeletal muscle relies on muscle satellite cells (MuSCs) and their interplay with different cell types within the niche. Yet, our understanding of the cells that compose the skeletal muscle tissue is limited and molecular definitions of the principal cell types are lacking. Using a combined approach of single-cell RNA-sequencing and mass cytometry, we precisely mapped the different cell types in adult skeletal muscle tissue and highlighted previously overlooked populations. We identified known functional populations, characterized their gene signatures, and determined key markers. Among the ten main cell populations present in skeletal muscle, we found an unexpected complexity in the interstitial compartment and identified two new cell populations. One express the transcription factor Scleraxis and generate tenocyte-like cells. The second express smooth muscle and mesenchymal cell markers (SMMCs). While distinct from MuSCs, SMMCs are endowed with myogenic potential and promote MuSC engraftment following transplantation. Our high-dimensional single-cell atlas uncovers principles of an adult tissue composition and can be exploited to reveal unknown cellular sub-fractions that contribute to tissue regeneration.
Effect of Reduced Processing on Recovery of Foodborne Pathogens from Hot-Boned Broiler Meat and Skin.

Recovery of pathogens from breast meat, thigh meat and skin from scalded, defeathered but uneviscerated broiler carcasses with and without spray washing was compared to recovery from breast meat, thigh meat and skin from fully processed, chilled carcasses (controls). The incidence of coagulase-positive staphylococci was not significantly different on meat and skin from both uneviscerated carcasses with and without a spray washing compared to meat and skin from fully processed carcasses. The incidence of Clostridium perfringens was not significantly different on skin, breast and thigh meat for any of the sampling sources except that incidence on meat from control breasts was lower than on breast meat from uneviscerated carcasses without spray-washing; and incidence on meat from control thighs was lower than on meat from spray-washed, uneviscerated carcasses. Salmonella incidence was higher on both breast and thigh meat from fully processed control carcasses than from uneviscerated unwashed carcasses. When uneviscerated carcasses were spray-washed after defeathering, the incidence of Salmonella was not significantly different on breast meat, and significantly lower on thigh meat than on these meats from fully processed control carcasses. Skin from fully processed control carcasses had a higher incidence of Salmonella than did skin from uneviscerated, unwashed carcasses, but not skin from uneviscerated, spray-washed carcasses. Reducing the number of stages of processing significantly reduced the incidence of Salmonella but not of coagulase-positive staphylococci or Clostridium perfringens .