Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic Context and Biochemical Characterization of the IMP-18 Metallo- (cid:1) -Lactamase Identiﬁed in a Pseudomonas aeruginosa Isolate from the United States (cid:1)

The production of metallo- (cid:1) -lactamase (MBL) is an important mechanism of resistance to (cid:1) -lactam antibiotics, including carbapenems. Despite the discovery and emergence of many acquired metallo- (cid:1) -lacta-mases, IMP-type determinants (now counting at least 27 variants) remain the most prevalent in some geographical areas. In Asian countries, and notably Japan, IMP-1 and its closely related variants are most widespread. Some other variants have been detected in other countries and show either an endemic (e.g., IMP-13 in Italy) or sporadic (e.g., IMP-12 in Italy or IMP-18 in the United States) occurrence. The IMP-18-producing Pseudomonas aeruginosa strain PS 297 from the southwestern United States carried at least two class 1 integrons. One was identical to In 51 , while the other, named In 133 and carrying the bla IMP-18 gene cassette in the third position, showed an original array of ﬁve gene cassettes, including aacA7 , qacF , aadA1 , and an unknown open reading frame (ORF). Interestingly. In 133 differed signiﬁcantly from In 96 , the bla IMP-18 - carrying integron identiﬁed in a P. aeruginosa isolate from Mexico. The meropenem and ertapenem MIC values were much lower for Escherichia coli strains producing IMP-18 (0.06 and 0.12 (cid:2) g/ml, respectively) than for strains producing IMP-1 (2 (cid:2) g/ml for each). Kinetic data obtained with the puriﬁed enzyme revealed lower turnover rates of IMP-18 than of other IMP-type enzymes with most substrates.
Improving Hyperlocal Air Quality in Cities Impact of vegetation on pollutants concentration at pedestrian level

: Recent estimates published by WHO report that in 2016 outdoor air pollution caused 4.2 million premature deaths worldwide and urged urban planners, policymakers, and environmentalist to make health and wellbeing their number one priority when designing cities. In view of this, the current paper explores the effectiveness of trees and vegetation in dispersing air pollutants at pedestrian level by administering detailed fieldwork and spot measurement of both pollutants and microclimatic parameters close to one of the most polluted roads in London (Euston Road); followed by modelling a variety of real-life scenarios by using computational simulation application for validation and prediction. Whilst many studies agree in general on the mitigation of urban air pollutants by vegetation, the result of the current study contradicts this common understanding and demonstrates drastic increases in the concentration of particulate matters in the vicinity of trees. The results highlight that trees reduce wind velocity and air movement, causing pollutants to trap inside urban canyons. Therefore, planting more trees does not necessarily mean less pollution, at least locally. Instead, to alleviate air quality problems, more attention should be given to vegetation configuration, type, scale and most importantly, their locations and distributions within active urban pockets.
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: Objective: Primary endocrine therapy (PET) is an alternative to surgery for oestrogen receptor positive operable breast cancer in some older women. However the decision to offer PET involves complex trade-offs and is influenced by both patient choice and healthcare professional (HCP) preference. This study aimed to compare the views of patients and HCPs about this decision and explore decision making (DM) preferences and whether these are taken into account during consultations. Methods: This multicentre, UK, mixed methods study had 3 components: (1) questionnaires to older women undergoing counselling about breast cancer treatment options which assessed their DM preferences and realities; (2) qualitative interviews with older women with operable breast cancer offered a choice of either surgery or PET and (3) qualitative interviews with HCPs (both of which focused on DM preferences in this setting). Results: Thirty-three patients and 34 HCPs were interviewed. A range of opinions about patient involvement in DM were identified. Patients indicated varying preferences for DM involvement which were variably taken into account by HCPs. These qualitative findings were broadly supported by the questionnaire results. Most patients (536/729; 73.5%) achieved their preferred DM style, however the remainder felt that their DM preferences had not been taken into consideration. Conclusions: These results suggest that whilst many older women achieve their desired level of DM engagement, some do not, raising the possibility that they may be making choices which are not concordant with their treatment preferences.
Coordinate Regulation of TPL-2 and NF- (cid:1) B Signaling in Macrophages by NF- (cid:1) B1 p105

The role of I (cid:1) B kinase (IKK)-induced proteolysis of NF- (cid:1) B1 p105 in innate immune signaling was investigated using macrophages from Nfkb1 SSAA/SSAA mice, in which the IKK target serines on p105 are mutated to alanines. We found that the IKK/p105 signaling pathway was essential for TPL-2 kinase activation of extracellular signal-regulated kinase (ERK) mitogen-activate protein (MAP) kinase and modulated the activation of NF- (cid:1) B. The Nfkb1 SSAA mutation prevented the agonist-induced release of TPL-2 from its inhibitor p105, which blocked activation of ERK by lipopolysaccharide (LPS), tumor necrosis factor (TNF), CpG, tripalmitoyl-Cys-Ser-Lys (Pam 3 CSK), poly(I · C), ﬂagellin, and R848. The Nfkb1 SSAA mutation also prevented LPS-induced processing of p105 to p50 and reduced p50 levels, in addition to decreasing the nuclear translocation of RelA and cRel. Reduced p50 in Nfkb1 SSAA/SSAA macrophages signiﬁcantly decreased LPS induction of the I (cid:1) B (cid:2) -regulated Il6 and Csf2 genes. LPS upregulation of Il12a and Il12b mRNAs was also impaired although speciﬁc blockade of TPL-2 signaling increased expression of these genes at late time points. Activation of TPL-2/ERK signaling by IKK-induced p105 proteolysis, therefore, induced phosphorylation in p105) suggested that LPS activation of TPL-2 MEK activity and from p105 IKK of p105. this, ret-roviral of FL-p105 S927A,S932A in Nfkb1 macrophages rescue LPS activation of ERK, in to WT FL-p105 these results suggested a role for IKK TPL-2/ERK signaling that p105 IKK of knock-in mice SSAA S935A p105) phosphorylated IKK. In the study, we used these mice to investigate the function of IKK-induced NF- B1 p105 proteolysis in NF- (cid:1) B and TPL-2/ERK activation in macrophages during an innate immune The Nfkb1 SSAA p105 p50 p105
Liver X receptor (cid:2) induces 17 (cid:3) -hydroxysteroid dehydrogenase-13 expression through SREBP-1c

Liver X receptor (cid:2) induces 17 (cid:3) -hydroxysteroid dehydrogenase-13 expression through SREBP-1c. E357–E367, X receptors, including LXR (cid:2) and LXR (cid:3) , are known to be master regulators of liver lipid metabolism. Activation of LXR (cid:2) increases hepatic lipid storage in lipid droplets (LDs). 17 (cid:3) -Hydroxysteroid dehydrogenase-13 (17 (cid:3) -HSD13), a recently tivity was normalized to the (cid:3) -galactosidase activity as measured by a kit. Chromatin immunoprecipitation assay. Chromatin immunoprecipitation (ChIP) assay was carried out by using the Chromatin Immuno- precipitation Kit (Thermo). HepG2 cells were treated with T0901317 or DMSO for 24 h, and ChIP assay was performed. 3 (cid:9) 10 5 cells were used for each ChIP assay. Anti-SREBP-1 antibody and normal mouse IgG (negative control) were used for immunoprecipitation of protein-DNA complexes. Precipitated DNA was analyzed by PCR using the following primers: 5-TATTTGAGGTGTTTCAAACC-3 (sense) and 5-TCACAGAGCTCTAGGGAAGG-3 (antisense). Ampliﬁcation products were then examined by electrophoresis.
Remarks on a recent result by Paul Skoufranis

We give a different proof of Paul Skoufranis's very recent result showing that the strong convergence of possibly non-commutative random variables $X^{(k)}\to X$ is stable under reduced free product with a fixed non-commutative random variable $Y$. In fact we obtain a more general fact: assuming that the families $X^{(k)}={X_i^{(k)}}$ and $Y^{(k)}={Y_j^{(k)}}$ are *-free as well as their limits (in moments) $X ={X_i}$ and $Y ={Y_j}$, the strong convergences $X^{(k)}\to X$ and $Y^{(k)}\to Y$ imply that of ${X^{(k)},Y^{(k)}}$ to ${X,Y}$. Phrased in more striking language: the reduced free product is "continuous" with respect to strong convergence. The analogue for weak convergence (i.e. convergence of all moments) is obvious.
Holographic Duals of D = 3 N = 4 Superconformal Field Theories

We ﬁnd the warped AdS 4 (cid:110) K type-IIB supergravity solutions holographically dual to a large family of three dimensional N = 4 superconformal ﬁeld theories labeled by a pair ( ρ, ˆ ρ ) of partitions of N . These superconformal theories arise as renormalization group ﬁxed points of three dimensional mirror symmetric quiver gauge theories, denoted by T ρ ˆ ρ ( SU ( N )) and T ˆ ρρ ( SU ( N )) respectively. We give a supergravity derivation of the conjectured ﬁeld theory constraints that must be satisﬁed in order for these gauge theories to ﬂow to a non-trivial supersymmetric ﬁxed point in the infrared. The exotic global symmetries of these superconformal ﬁeld theories are precisely realized in our explicit supergravity description.
insects Effect of Temperature on Heart Rate for Lucilia sericata (syn Phaenicia sericata ) and Drosophila melanogaster with Altered Expression of the TrpA1 Receptors

Simple Summary: Thermal receptors detect temperature changes and can alter the activity of the cells. A subtype referred to as TrpA1 responds to increased temperature and increases expression in the heart of mammals when the heart is injured or is reduced in oxygen. It is not known if this is beneﬁcial or detrimental to the heart. Thus, we examined the effect on heart activity of altering heart expression of TrpA1 in larval fruit ﬂies at varied temperatures. Hearts of normal larvae stopped beating at 37 ◦ C but hearts expressing high levels of TrpA1 stopped beating at 30 ◦ C. In contrast, unmodiﬁed larvae of a blowﬂy species that grows at higher temperatures showed increased heart rate with increased temperature to 37 ◦ C. It is not known if blowﬂies alter their expression of the thermal receptors. Thermal receptors can also be activated by physical stretch. Thus, it is possible an increase in expression in mammalian hearts within a narrow temperature range could be helpful in maintaining heart rate, as activation of TrpA1 receptors may be modulated by the stretching and relaxing of the heart itself. More research is needed in examining the function of TrpA1 receptors in mammalian hearts. Abstract: The transient receptor potential (TrpA—ankyrin) receptor has been linked to pathological conditions in cardiac function in mammals. To better understand the function of the TrpA1 in regulation of the heart, a Drosophila melanogaster model was used to express TrpA1 in heart and body wall muscles. Heartbeat of in intact larvae as well as hearts in situ, devoid of hormonal and neural input, indicate that strong over-expression of TrpA1 in larvae at 30 or 37 ◦ C stopped the heart from beating, but in a diastolic state. Cardiac function recovered upon cooling after short exposure to high temperature. Parental control larvae (UAS-TrpA1) increased heart rate transiently at 30 and 37 ◦ C but slowed at 37 ◦ C within 3 min for in-situ preparations, while in-vivo larvae maintained a constant heart rate. The in-situ preparations maintained an elevated rate at 30 ◦ C. The heartbeat in the TrpA1-expressing strains could not be revived at 37 ◦ C with serotonin. Thus, TrpA1 activation may have allowed enough Ca 2+ inﬂux to activate K( Ca ) channels into a form of diastolic stasis. TrpA1 activation in body wall muscle conﬁrmed a depolarization of membrane. In contrast, blowﬂy Lucilia sericata (syn Phaenicia sericata ) larvae increased heartbeat at 30 and 37 ◦ C, demonstrating greater cardiac thermotolerance.
Symptomatic and Asymptomatic Viral Recrudescence in Solid-Organ Transplant Recipients and Its Relationship with the Antigen-Speciﬁc CD8 (cid:1) T-Cell Response (cid:1)

Using ex vivo antigen-speciﬁc T-cell analysis, we found that symptomatic cytomegalovirus recrudescence in transplant recipients was coincident with reduced expression of gamma interferon (IFN- (cid:1) ) by virus-speciﬁc CD8 (cid:2) T cells and an up-regulation of CD38 expression on these T cells, although there was no signiﬁcant change in the absolute number of virus-speciﬁc cells (as assessed by major histocompatibility complex-peptide multimers). In contrast, HLA class I-matched transplant patients with asymptomatic viral recrudescence showed increased expansion of antigen-speciﬁc T cells and highly stable IFN- (cid:1) expression
Depression and anxiety during the COVID-19 pandemic in Saudi Arabia: a cross-1 study

25 Aims: The emergence of the COVID-19 global pandemic, with a high transmission and mortality 26 rate, has created an extraordinary crisis worldwide. Such an unusual situation may have an 27 undesirable impact on the mental health of individuals which, in turn, may influence their 28 outcomes. This study aimed to explore the influence of the COVID-19 pandemic on the 29 psychological disposition of residents of the Kingdom of Saudi Arabia. 30 Methods: A cross-sectional study using an online survey was conducted in Saudi Arabia 31 between 27 March and 27 April 2020. The Patient Health Questionnaire (PHQ-9) and 32 Generalised Anxiety Disorder-7 (GAD-7) were used to assess depression and anxiety. Logistic 33 regression analysis was used to identify predictors of these. 34 Results: A total of 2,081 individuals participated in the study. The prevalence of depression and 35 anxiety among the study participants was 9.4% and 7.3%, respectively. Non-Saudi residents, 36 individuals aged 50 years and above, divorced people, retired people, university students, and 37 those with an income between 2,000 and 10,000 SR were at higher risk of developing 38 depression. Saudi individuals, married people, the unemployed, and those with a high income (> 39 10,000 RS) were at higher risk of developing anxiety. 40 Conclusion: We found that there is a wide range of Saudi residents who are at higher risk of 41 developing mental illness during the current COVID-19 pandemic. Policymakers and mental 42 healthcare providers are advised to provide continuous monitoring of the psychological 43 consequences during this pandemic and provide the required health support.
Dietary glycemic index, dietary glycemic load, blood lipids, and C-reactive protein share how this access benefits you. Your story matters

Carbohydrate quantity and quality may influence risk of cardiovascular disease through blood lipid concentrations and inflammation. We measured dietary glycemic index (GI) and dietary glycemic load (GL) among 18,137 healthy women ≥ 45 years old without diagnosed diabetes using a food-frequency questionnaire. We assayed fasting total, HDL, and LDL cholesterol, LDL:HDL cholesterol ratio, triacylglycerols (TG), and C-reactive protein (CRP). We evaluated associations with dietary GI and GL using a cross-sectional design, adjusting for age, body mass index, lifestyle factors, and other dietary factors. Dietary GI was significantly associated with HDL and LDL cholesterol, LDL:HDL cholesterol ratio, TG, and CRP (comparing bottom quintile difference in HDL cholesterol = -2.6 mg/dL, LDL cholesterol = 2.2 mg/dL, LDL:HDL cholesterol ratio 0.16, TG 12 mg/dL, and CRP 0.21 mg/L). Dietary GL was associated with HDL cholesterol, LDL:HDL cholesterol and TG
OPTICALLY STIMULATED LUMINESCENCE DATING USING QUARTZ: REMARKS AND A PLEA FOR FAIRNESS

The optical stimulated luminescence (OSL) dating is a steadily developed method in the fields of archaeology, geoarchaeology and earth sciences. The trapped electrons in lattice defects of suitable minerals during irradi-ation by natural radioisotopes throughout the time and the emitted luminescent light after agitation by optical radiation in the lab (or by daylight), are physical processes which determine the total equivalent (to a laboratory dose) dose (in Grays). The intentional or accidental light exposure to light and the thermal agitation sets luminescence clock to zero for a mineral within a material to be dated. Excellent publications (either articles or books) and reviews on the OSL dating have been made. The objective coverage of essential novel applications and basic research accompanied by an ethic unbiased pace is a prerequisite for scientific integrity of every publication. A recent Nature Reviews Method Primers on OSL dating of quartz was the stimulus for the present constructive and supplementary information and serves also as a plea for fairness.
1-Neonatal antibiotic use at a district and teaching hospital in Rwanda – a retrospective descriptive study

INTRODUCTION : Infection remains a significant cause of death in the neonatal period. Antibiotics are lifesaving in genuine infection but have been shown to be detrimental if overused in neonates who have no evidence of infection. This study aims to describe baseline length and choice of antibiotic used in a district and referral teaching hospital neonatal unit in Kigali, Rwanda. METHODS: A retrospective, descriptive chart review was conducted among neonates admitted from January 2015 to December 2017 to Neonatology Units at Muhima District Hospital (MHD) and University Teaching Hospital of Kigali (UTHK). RESULTS: Convenience sampling was used to identify 178 neonates who were enrolled from MDH (n=112) and UTHK (n=66). 88% of neonates received antibiotics, for a median of 6 days. Neonates spent a mean of 72% of their admission-period on intravenous antibiotics. The most common first-line antibiotics were ampicillin (100%) and gentamicin (97%). Blood cultures were ordered in 70 cases (41.2%) and a positive culture was found in 16 cases, with Klebsiella species and Staphylococcus aureus the only organisms cultured. CONCLUSION: Infection remains a significant problem for neonates. With increasing challenges from antibiotic resistance, the results of this study demonstrate the need for antibiotic stewardship programs in Rwandan Neonatology Units.
Multiple metabolisms constrain the anaerobic nitrite budget in the Eastern Tropical South Paci ﬁ c Global Biogeochemical Cycles

The Eastern Tropical South Paci ﬁ c is one of the three major oxygen de ﬁ cient zones (ODZs) in the global ocean and is responsible for approximately one third of marine water column nitrogen loss. It is the best studied of the ODZs and, like the others, features a broad nitrite maximum across the low oxygen layer. How the microbial processes that produce and consume nitrite in anoxic waters interact to sustain this feature is unknown. Here we used 15 N-tracer experiments to disentangle ﬁ ve of the biologically mediated processes that control the nitrite pool, including a high-resolution pro ﬁ le of nitrogen loss rates. Nitrate reduction to nitrite likely depended on organic matter ﬂ uxes, but the organic matter did not drive detectable rates of denitri ﬁ cation to N 2 . However, multiple lines of evidence show that denitri ﬁ cation is important in shaping the biogeochemistry of this ODZ. Signi ﬁ cant rates of anaerobic nitrite oxidation at the ODZ boundaries were also measured. Iodate was a potential oxidant that could support part of this nitrite consumption pathway. We additionally observed N 2 production from labeled cyanate and postulate that anammox bacteria have the ability to harness cyanate as another form of reduced nitrogen rather than relying solely on ammoni ﬁ cation of complex organic matter. The balance of the ﬁ ve anaerobic rates measured — anammox, denitri ﬁ cation, nitrate reduction, nitrite oxidation, and dissimilatory nitrite reduction to ammonium — is suf ﬁ cient to reproduce broadly the observed nitrite and nitrate pro ﬁ les in a simple one-dimensional model but requires an additional source of reduced nitrogen to the deeper ODZ to avoid ammonium overconsumption.
CLEP_A_242080 527..535

Clinical Research Institute, American University of Beirut, Beirut, Lebanon; Wolfson Palliative Care Research Centre, Hull York Medical School, University of Hull, Hull, UK; Department of Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, Canada; Pharmaceutical Sciences Post Graduate Course, University of Sorocaba, UNISO, Sorocaba, Sao Paulo, Brazil; Department of Medicine, University of Toronto, Toronto, Ontario, Canada; Chinese Evidence-Based Medicine Center, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; Departments of Medicine and Biomedical & Health Informatics, University of Missouri-Kansas City, Kansas City, MO, USA; Department of Evaluative Clinical Sciences, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada; Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Department of Anesthesia, McMaster University, Hamilton, Canada; The Michael G. DeGroote National Pain Centre, McMaster University, Hamilton, Canada; Chronic Pain Centre of Excellence for Canadian Veterans, Hamilton, Canada; Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Medicine, McMaster University, Hamilton, Canada Background: How systematic review authors address missing data among eligible primary
Evaluation of Factors Inﬂuencing the Inclusion of Indigenous Plants for Food Security among Rural Households in the North West Province of South Africa

: Underutilised indigenous plants can support and strengthen the existing food system, as they are considered as socio-economically and environmentally appropriate. These plants generally adapt to marginal conditions, which is essential for a resilient agriculture and sustainable food systems. The current study relied on food security and indigenous plants data collected from some selected rural households from the North West Province of South Africa. The utilised data were collected through a multi-stage sampling technique with the aid of a pre-tested semi-structured questionnaire, while descriptive methods Foster–Greer–Thorbecke (FGT) and binary logistic regression were used for data analysis. The models produced a good ﬁt for the data, and the computed F-value was statistically signiﬁcant ( p < 0.01). The study examined socio-economic and food security status based on the knowledge and the perception of indigenous plants by the households. The incidence of food insecurity ( θ 0 ) was 0.4060, indicating that 40.6% of the participants were food insecure while 59.4% were food secured. Binary logistic regression results indicate that factors such as age, gender, educational attainment, inclusion of indigenous plants in diet, food expenditure, and access in the study area impacted results. It was also evident that the participants had considerable knowledge of indigenous plants. However, these indigenous plants were not cultivated or included in the diet by the majority of the participants. The formulation of appropriate holistic policies that support the incorporation of the indigenous plants into the food system is recommended.