Miracle-dz/UniFAR-FTUs huggingface dataset:
Chemically speciated mass size distribution, particle density, shape and origin of non-refractory PM<sub>1</sub> measured at a rural background site in central Europe

Abstract. Seasonal variability of non-refractory PM1 (NR-PM1) was studied at a rural background site (National Atmospheric Observatory Košetice – NAOK) in the Czech Republic to investigate the effect of regional and long-range atmospheric transport in central Europe. NR-PM1 measurements were performed by compact time-of-flight aerosol mass spectrometry (C-ToF-AMS), and the chemically speciated mass size distributions, density, shape, and origin were discussed. Average PM1 concentrations, calculated as the sum of the NR-PM1 and the equivalent black carbon (eBC) concentrations measured by an aethalometer (AE), were 8.58 ± 3.70 µg m−3 in summer and 10.08 ± 8.04 µg m−3 in winter. Organics were dominant during both campaigns (summer/winter: 4.97 ± 2.92/4.55 ± 4.40 µg m−3), followed by SO42-in summer (1.68 ± 0.81/1.36 ± 1.38 µg m−3) and NO3- in winter (0.67 ± 0.38/2.03 ± 1.71 µg m−3). The accumulation mode dominated the average mass size distribution during both seasons, with larger particles of all species measured in winter (mode diameters: Org: 334/413 nm, NO3-: 377/501 nm, SO42-: 400/547 nm, and NH4+: 489/515 nm) indicating regional and long-range transport. However, since the winter aerosols were less oxidized than the summer aerosols (comparing fragments f44 and f43), the importance of local sources in the cold part of the year was still enough to be considered. Although aged continental air masses from the south-east (SE) were rare in summer (7 %), they were related to the highest concentrations of PM1, eBC, and all NR-PM1 species, especially SO42- and NH4+. In winter, slow continental air masses from the south-west (SW) (44 %) were linked to inversion conditions over central Europe and were associated with the highest concentrations among all NR-PM1 species as well as PM1 and eBC. Average PM1 material density (ρm) corresponded to higher inorganic contents in both seasons (summer: ∼ 1.30 g cm−3 and winter: ∼ 1.40 g cm−3). During episodes of higher mass concentrations ρm ranged from 1.30–1.40 g cm−3 in summer and from 1.30–1.50 g cm−3 in winter. The dynamic shape factors (χ) decreased slightly with particle mobility diameter (Dm) in both seasons. This study provides insights into the seasonal effects and air mass variability on aerosol particles, focusing on episodes of high mass and number concentrations measured at a central European rural background site.
Can Individuals with Suboptimal Antibody Responses to Conventional Antiviral Vaccines Acquire Adequate Antibodies from SARS-CoV-2 mRNA Vaccination?

In Japan, healthcare workers (HCWs) are vaccinated against measles, rubella, chickenpox, mumps, and hepatitis B to prevent nosocomial infection; however, some do not produce sufficient antibodies (“suboptimal responders”). This study compared immune responses to a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 mRNA) vaccine among HCWs with normal and suboptimal responses to conventional vaccines. In this prospective cohort study, 50 HCWs received two doses of BNT162b2 mRNA vaccine 3 weeks apart. SARS-CoV-2 anti-spike antibodies were measured 11 times, starting before the first vaccination and ending 5 months after the second vaccination. Antibody titers of four suboptimal and 46 normal responders were compared. SARS-CoV-2 neutralizing antibody activity was measured twice in suboptimal responders, 1 week/1 month and 5 months after the second vaccination. The SARS-CoV-2 anti-spike antibody was detectable in the samples from suboptimal and normal responders at each timepoint after vaccination. Suboptimal responders exhibited SARS-CoV-2 neutralizing antibody activity 1 week/1 month as well as 5 months after the second vaccination; however, activity was slightly reduced at 5 months. Our findings show that suboptimal responders do acquire adequate SARS-CoV-2 anti-spike and SARS-CoV-2 neutralizing antibodies from vaccination to prevent SARS-CoV-2. SARS-CoV-2 mRNA vaccines should thus be recommended for both normal and suboptimal responders to conventional vaccines.
Prevalence of Tuberculosis, Rifampicin Resistant Tuberculosis and Associated Risk Factors in Presumptive Tuberculosis Patients Attending Some Hospitals in Kaduna, Nigeria

The accurate diagnosis of tuberculosis (TB), detection of drug‐resistant TB and knowledge of the associated risk factors can reduce transmission of TB in the community. Therefore this study was carried out to determine the prevalence of Mycobaterium tuberculosis(MTB) and its resistance to Rifampicin using GeneXpert in presumptive tuberculosis patients attending some hospitals in Kaduna, Nigeria. A total of 198 sputum samples were collected and screened from participants who had been administered questionnaires using Ziehl Neelsen Acid Fast Bacilli (ZN AFB) method and GeneXpert molecular technique. Data obtained showed that using the Zn AFB staining technique, MTB was seen in 65 sputum test results but with the GeneXpert technique, MTB was further detected in 15 sputum sample, bringing the total to 80, thus confirming that GeneXpert was more sensitive in detecting TB compared to the ZN AFB method. The overall prevalence of TB and Rifampicin resistant TB (RRTB) was 40.4% and 1.25% respectively. A higher prevalence rate was reported among male subjects (47.2%), age group 31‐40 years (50.9%), HIV positive subjects (54.5%), those that smoke cigarettes (72.2%) and those that has previous contact with someone living with TB infection (82.4%). Assay for statistical significance showed that independent variables that were significantly associated with the rate of occurrence of TB (p<0.05 at 95% C.I) were Age, Gender, HIV Status, smoking behaviour and previous contact with someone having TB. The only RRTB case detected was a male who has had a previous contact with someone living with TB infection. This study indicates a low burden of rifampicin resistant tuberculosis among the participants. It is thus concluded that rapid detection of TB and Rifampicin resistant strains using GeneXpert and other molecular techniques for TB diagnosis in clinical settings is key to early access to the right therapy; it will also improve treatment outcomes and decrease transmission rates.
siRNA against CD40 delivered via a fungal recognition receptor ameliorates murine acute graft‐versus‐host disease

Acute graft‐versus‐host disease (aGvHD) remains a major threat to a successful outcome after allogeneic hematopoietic stem cell transplantation (HSCT). Although antibody‐based targeting of the CD40/CD40 ligand costimulatory pathway can prevent aGvHD, side effects hampered their clinical application, prompting a need for other ways to interfere with this important dendritic T‐cell costimulatory pathway. Here, we used small interfering RNA (siRNA) complexed with β‐glucan allowing the binding and uptake of the siRNA/β‐glucan complex (siCD40/schizophyllan [SPG]; chemical modifications called NJA‐312, NJA‐302, and NJA‐515) into Dectin1+ cells, which recognize this pathogen‐associated molecular pattern receptor. aGvHD was induced by the transplantation of splenocytes and bone marrow cells from C57BL/6J into CBF1 mice. Splenic dendritic cells retained Dectin1 expression after HSCT but showed lower expression after irradiation. The administration of siCD40/SPG, NJA‐312, and NJA‐302 ameliorated aGvHD‐mediated lethality and tissue damage of spleen and liver, but not skin. Multiple NJA‐312high injections prevented aGvHD but resulted in early weight loss in allogeneic HSCT mice. In addition, NJA‐312 treatment caused delayed initial donor T and B‐cell recovery but resulted in stable chimerism in surviving mice. Mechanistically, NJA‐312 reduced organ damage by suppressing CCR2+, F4/80+, and IL17A‐expressing cell accumulation in spleen, liver, and thymus but not the skin of mice with aGvHD. Our work demonstrates that siRNA targeting of CD40 delivered via the PAMP‐recognizing lectin Dectin1 changes the immunological niche, suppresses organ‐specific murine aGvHD, and induces immune tolerance after organ transplantation. Our work charts future directions for therapeutic interventions to modulate tissue‐specific immune reactions using Pathogen‐associated molecular pattern (PAMP) molecules like 1,3‐β‐glucan for cell delivery of siRNA.
Acridine Based N-Acylhydrazone Derivatives as Potential Anticancer Agents: Synthesis, Characterization and ctDNA/HSA Spectroscopic Binding Properties

A series of novel acridine N-acylhydrazone derivatives have been synthesized as potential topoisomerase I/II inhibitors, and their binding (calf thymus DNA—ctDNA and human serum albumin—HSA) and biological activities as potential anticancer agents on proliferation of A549 and CCD-18Co have been evaluated. The acridine-DNA complex 3b (-F) displayed the highest Kb value (Kb = 3.18 × 103 M−1). The HSA-derivatives interactions were studied by fluorescence quenching spectra. This method was used for the calculation of characteristic binding parameters. In the presence of warfarin, the binding constant values were found to decrease (KSV = 2.26 M−1, Kb = 2.54 M−1), suggesting that derivative 3a could bind to HSA at Sudlow site I. The effect of tested derivatives on metabolic activity of A549 cells evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT assay decreased as follows 3b(-F) > 3a(-H) > 3c(-Cl) > 3d(-Br). The derivatives 3c and 3d in vitro act as potential dual inhibitors of hTopo I and II with a partial effect on the metabolic activity of cancer cells A594. The acridine-benzohydrazides 3a and 3c reduced the clonogenic ability of A549 cells by 72% or 74%, respectively. The general results of the study suggest that the novel compounds show potential for future development as anticancer agents.
Relativistic Electron Precipitation Driven by Nonlinear Resonance With Whistler‐Mode Waves

Electron losses from the outer radiation belt are typically attributed to resonant electron scattering by whistler‐mode waves. Although the quasi‐linear diffusive regime of such scattering is well understood, the observed waves are often quite intense and in the nonlinear regime of resonant wave‐particle interaction. Such nonlinear resonant interactions are still being actively studied due to their potential for driving fast precipitation. However, direct observations of nonlinear resonance of whistler‐mode waves with electron distributions are scarce. Here, we present evidence for such resonance with high‐resolution electron energy and pitch angle spectra acquired at low‐altitudes by the dual Electron Losses and Fields INvestgation (ELFIN) CubeSats combined with conjugate measurements of equatorial plasma parameters, wave properties, and electron energy spectra by the Time History of Events and Macroscale Interactions during Substorms and Magnetospheric MultiScale missions. ELFIN has obtained numerous conjunction events exhibiting whistler wave driven precipitation; in this study, we present two such events which epitomize signatures of nonlinear resonant scattering. A test particle simulation of electron interactions with intense whistler‐mode waves prescribed at the equator is employed to directly compare modeled precipitation spectra with ELFIN observations. We show that the observed precipitating spectra match expectations to within observational uncertainties of wave amplitude for reasonable assumptions of wave power distribution along the magnetic field line. These results indicate the importance of nonlinear resonant effects when describing intense precipitation patterns of energetic electrons and open the possibility of remotely investigating equatorial wave properties using just properties of precipitation energy and pitch angle spectra.
Effects of Salt Stress on Transcriptional and Physiological Responses in Barley Leaves with Contrasting Salt Tolerance

Salt stress negatively impacts crop production worldwide. Genetic diversity among barley (Hordeum vulgare) landraces adapted to adverse conditions should provide a valuable reservoir of tolerance genes for breeding programs. To identify molecular and biochemical differences between barley genotypes, transcriptomic and antioxidant enzyme profiles along with several morpho-physiological features were compared between salt-tolerant (Boulifa) and salt-sensitive (Testour) genotypes subjected to salt stress. Decreases in biomass, photosynthetic parameters, and relative water content were low in Boulifa compared to Testour. Boulifa had better antioxidant protection against salt stress than Testour, with greater antioxidant enzymes activities including catalase, superoxide dismutase, and guaiacol peroxidase. Transcriptome assembly for both genotypes revealed greater accumulation of differentially expressed transcripts in Testour compared to Boulifa, emphasizing the elevated transcriptional response in Testour following salt exposure. Various salt-responsive genes, including the antioxidant catalase 3, the osmoprotectant betaine aldehyde dehydrogenase 2, and the transcription factors MYB20 and MYB41, were induced only in Boulifa. By contrast, several genes associated with photosystems I and II, and light receptor chlorophylls A and B, were more repressed in Testour. Co-expression network analysis identified specific gene modules correlating with differences in genotypes and morpho-physiological traits. Overall, salinity-induced differential transcript accumulation underlies the differential morpho-physiological response in both genotypes and could be important for breeding salt tolerance in barley.
Clinical Evaluation of the Optical Filter for Autofluorescence Glasses for Oral Cancer Curing Light Exposed (GOCCLES®) in the Management of Potentially Premalignant Disorders: A Retrospective Study

Background: Any oral potentially malignant disorders (OPMDs) must be regularly monitored through clinical examination to detect any possible malignant transformation. Conventional intraoral exams, however, can be difficult because these conditions may resemble benign lesions. For this reason, several non-invasive diagnostic technologies have been developed to help the clinician in detecting and distinguishing between cancerous and benign lesions. Epithelial dysplasia can be considered the most important predictor of malignant evolution. Therefore, in this study we aim to evaluate the ability of an optical filter for autofluorescence Glasses for Oral Cancer Curing Light Exposed (GOCCLES®) and of toluidine blue staining in identifying dysplastic areas in patients with OPMDs. Methods: In this retrospective study, medical records, photographs and videos of 25 patients with oral lesions were analyzed. Forty-two biopsy samples in 25 patients with OPMDs and at least one suspicious oral mucosa lesion that were evaluated in white light, autofluorescence with optical filter GOCCLES®, toluidine blue staining and then biopsied with histopathological analysis were analyzed. Results: The sensitivity and specificity for the autofluorescence evaluation with GOCCLES® for identifying dysplasia or carcinoma were 66% and 48%, respectively. The positive and negative predictive values were 34% and 77%, respectively, and the accuracy was 53%. The sensitivity and specificity for toluidine blue staining were 91% and 68%, respectively. The positive and negative predictive values were 55% and 95%, respectively, and the accuracy was 75%. Conclusions: The optical filter for autofluorescence (GOCCLES®) and toluidine blue staining are simple, inexpensive, rapid and non-invasive procedures that can assist the clinician in distinguishing OPMDs from healthy mucosa but they are not able to distinguish benign and malignant lesions.
Gender, Police Culture, and Structured Ambivalence: Navigating ‘Fit' with the Brotherhood, Boys’ Club, and Sisterhood

Women are increasingly represented in policing; however, inclusion alone will not eradicate existing structural and cultural barriers to meaningful change. Insights from interviews with ninety-one Canadian women police of varied rank and tenure, demonstrate women’s experiences of structured ambivalence as they strategically deploy and resist gendered policing narratives of the Brotherhood, Boys’ Club, and Sisterhood to negotiate their own ‘fit. ’ In this way, they both challenge and reinforce gendered boundaries that create barriers to meaningful transformation. These findings demonstrate the need for change initiatives to address the complex and ever-shifting role of gender in policing organizations.
Data Collection for Dilute Protein Solutions via a Neutron Backscattering Spectrometer

Understanding protein functions requires not only static but also dynamic structural information. Incoherent quasi-elastic neutron scattering (QENS), which utilizes the highly incoherent scattering ability of hydrogen, is a powerful technique for revealing the dynamics of proteins in deuterium oxide (D2O) buffer solutions. The background scattering of sample cells suitable for aqueous protein solution samples, conducted with a neutron backscattering spectrometer, was evaluated. It was found that the scattering intensity of an aluminum sample cell coated with boehmite using D2O was lower than that of a sample cell coated with regular water (H2O). The D2O-Boehmite coated cell was used for the QENS measurement of a 0.8 wt. % aqueous solution of an intrinsically disordered protein in an intrinsically disordered region of a helicase-associated endonuclease for a fork-structured type of DNA. The cell was inert against aqueous samples at 283–363 K. In addition, meticulous attention to cells with small individual weight differences and the positional reproducibility of the sample cell relative to the spectrometer neutron beam position enabled the accurate subtraction of the scattering profiles of the D2O buffer and the sample container. Consequently, high-quality information on protein dynamics could be extracted from dilute protein solutions.
Relationship of Food Acceptance With Energy And Protein Consumption Levels of Santriwati

ABSTRACT Adolescent nutritional intake needs considered, especially those school in boarding, so that it can affect their food intake. Research objective was to determine relationship between food acceptance and level of energy and protein consumption of students. This study used a cross sectional study with quantitative approach. Population and research sample were students of VII and VIII grade Ummahatul Mukminin Islamic boarding school. Data were processed using SPSS version 25.0 for Windows. Results showed the students acceptance of food served was in moderate category, the average energy requirement students was 1754.58 kcal/day, while protein needs of 51.79 g/day, most of the energy and protein consumption levels of students were in normal category, All of the students energy and protein availability in normal category, students consumption level of energy and protein needs in weight deficit category, level availability of santriwati's energy and protein needs in deficit category. The conclusion of study is a significant relationship between food acceptance and level of energy and protein consumption students. Keywords : Acceptability, Level of Energy and Protein Consumption, Santriwati
Plasma Aldo-Keto Reductase Family 1 Member B10 as a Biomarker Performs Well in the Diagnosis of Nonalcoholic Steatohepatitis and Fibrosis

We found several blood biomarkers through computational secretome analyses, including aldo-keto reductase family 1 member B10 (AKR1B10), which reflected the progression of nonalcoholic fatty liver disease (NAFLD). After confirming that hepatic AKR1B10 reflected the progression of NAFLD in a subgroup with NAFLD, we evaluated the diagnostic accuracy of plasma AKR1B10 and other biomarkers for the diagnosis of nonalcoholic steatohepatitis (NASH) and fibrosis in replication cohort. We enrolled healthy control subjects and patients with biopsy-proven NAFLD (n = 102) and evaluated the performance of various diagnostic markers. Plasma AKR1B10 performed well in the diagnosis of NASH with an area under the receiver operating characteristic (AUROC) curve of 0.834 and a cutoff value of 1078.2 pg/mL, as well as advanced fibrosis (AUROC curve value of 0.914 and cutoff level 1078.2 pg/mL), with further improvement in combination with C3. When we monitored a subgroup of obese patients who underwent bariatric surgery (n = 35), plasma AKR1B10 decreased dramatically, and 40.0% of patients with NASH at baseline showed a decrease in plasma AKR1B10 levels to below the cutoff level after the surgery. In an independent validation study, we proved that plasma AKR1B10 was a specific biomarker of NAFLD progression across varying degrees of renal dysfunction. Despite perfect correlation between plasma and serum levels of AKR1B10 in paired sample analysis, its serum level was 1.4-fold higher than that in plasma. Plasma AKR1B10 alone and in combination with C3 could be a useful noninvasive biomarker for the diagnosis of NASH and hepatic fibrosis.
Combining implementation intentions and monetary incentives to reduce alcohol use: a failed generalization to a public bar context

ABSTRACT Background Implementation intentions have been demonstrated to reduce alcohol use in retrospective self-report measures. It remains unclear, however, whether they can moderate drinking in a challenging naturalistic context. We examined this by studying the effects of implementation intentions on alcohol use in a bar. Methods One hundred and twenty-one participants visiting a local bar were randomized over three conditions: control, motivational trigger, and motivational trigger plus implementation intentions. Those in the motivational trigger group were motivated to reduce drinking, and the combined intervention group made additional alcohol-related implementation intentions. We assessed participants’ pre- and post-intervention breath alcohol concentrations, their post-intervention drinking behavior over one hour, and measures of trait impulsivity and reward sensitivity. To predict post-intervention drinking, multiple hierarchical regression was used. Results Neither the motivational trigger nor the added implementation intentions reduced drinking. Across treatment groups, trait impulsivity predicted alcohol consumption. Conclusions The present study showed that motivational trigger and implementation intentions failed to reduce alcohol use in a naturalistic setting. Potential reasons, implications, and limitations are discussed. This first attempt demonstrates that implementation intentions aimed at moderation are not as easily implemented in a public high-risk context.
Evaluation of the Efficacy of Topically Applied Melatonin Gel as Adjunctive Therapy in Chronic Periodontitis; Randomized Control Trail

Aim: This study was conducted to assess the benefit of locally delivered melatonin gel as adjunctive to scaling and root planning (SRP) in the treatment of chronic periodontitis. Subjects and Methods: Forty chronic periodontitis selected patients ,divided into two groups. Group I: included 20 chronic periodontitis patients, treated by conventional periodontal therapy SRP combined with intra- pocket application of Melatonin gel once weekly for 1 month begin application at the second week after initial therapy. Group II: included 20 chronic periodontitis patients, treated by SRP combined with the injection of placebo, weekly for one month. The periodontal parameters were recorded at baseline, 1week, 1and 3 months.Gingival crevicular fluid (GCF) samples were collected and a quantitative measurement of receptor activator nuclear K ligand (RANKL) was carried out by using Enzyme-Linked Immunosorbent Assay (ELISA) at baseline, 1week, 1 and 3 months. Results: We found noticeable significant reduction in all clinical parameter and in the amount of GCF RANKL in group I in all evaluation period at 1 ,3-month over group II. Conclusion: The results proved the benefit of Melatonin as a promising adjunctive therapy in improving periodontal parameters. However, further long-term studies with large sample size are needed.