Miracle-dz/UniFAR-FTUs huggingface dataset:
Anxiety, depression, and stress in response to the coronavirus disease-19 pandemic.

Objective The objective of the study was to determine the state of anxiety, depression, and stress present in the society during the development of the 2019 coronavirus pandemic. Methods Mixed methods study; a three-section questionnaire was developed which included sociodemographic, perceptions, emotions, and behaviors related to the 2019 coronavirus pandemic, and two emotional assessment psychometric tests. The proportions and confidence intervals of the variables were calculated and compared using the Chi-square test. Results More than 40% of the subjects presented some degree of anxiety and 41.3% depression; the proportion of stress was < 30%. Of the subjects who experienced anxiety, 18.6% also had moderate-to-very severe depression or stress. Conclusion There are emotional indicators derived from the 2019 coronavirus pandemic in almost half of the study population. The identification and timely treatment of these states could lessen the psychological impact due to 2019 coronavirus.
Multifaceted Roles of TRIM Proteins in Colorectal Carcinoma

Colorectal cancer (CRC) is one of the most frequently diagnosed tumor in humans and one of the most common causes of cancer-related death worldwide. The pathogenesis of CRC follows a multistage process which together with somatic gene mutations is mainly attributed to the dysregulation of signaling pathways critically involved in the maintenance of homeostasis of epithelial integrity in the intestine. A growing number of studies has highlighted the critical impact of members of the tripartite motif (TRIM) protein family on most types of human malignancies including CRC. In accordance, abundant expression of many TRIM proteins has been observed in CRC tissues and is frequently correlating with poor survival of patients. Notably, some TRIM members can act as tumor suppressors depending on the context and the type of cancer which has been assessed. Mechanistically, most cancer-related TRIMs have a critical impact on cell cycle control, apoptosis, epithelial–mesenchymal transition (EMT), metastasis, and inflammation mainly through directly interfering with diverse oncogenic signaling pathways. In addition, some recent publications have emphasized the emerging role of some TRIM members to act as transcription factors and RNA-stabilizing factors thus adding a further level of complexity to the pleiotropic biological activities of TRIM proteins. The current review focuses on oncogenic signaling processes targeted by different TRIMs and their particular role in the development of CRC. A better understanding of the crosstalk of TRIMs with these signaling pathways relevant for CRC development is an important prerequisite for the validation of TRIM proteins as novel biomarkers and as potential targets of future therapies for CRC.
A 10 year comparative study of caesarean deliveries using the Robson 10 group classification system in a university hospital in Austria

Objective The Robson ten group classification system is used as a global standard for assessing, monitoring and comparing caesarean delivery (CD) rates within and between maternity services. Our objective was to compare the changes of CD rates at our institution between the years 2008–2010 and 2017–2019 using the Robson ten group classification system. Study design Data was collected retrospectively and all women were classified using the obstetric concepts and parameters described in the Robson ten group classification system. Results During 2008–2010 7,832 deliveries were performed, increasing to 9,490 in 2017–2019. The CD rate also increased from 29.1% to 32.2% (p<.05) during this 10 year period. In both observed periods group 5 (single cephalic multiparous women at term with a previous CD) was the largest contributor to the overall CD rate accounting for 20.2% of all CD during 2008–2010 and increasing to 26.9% in 2017–2019 (p<.001). The overall size of group 5 also increased from 8.3% to 11.6% (p<.001). Furthermore, an increase in CD rate in group 7 (multiparous women with a single breech pregnancy, including women with a uterine scar) from 92.9% to 98.2% (p = .752) could be observed. In group 8 (women with multiple pregnancies, including women with a uterine scar) a slight shift towards vaginal delivery (VD) can be reported with CD rates decreasing from 82% to 79.2% (p = .784). There was no observed difference with CD rates in group 1 although the group size decreased from 29.4% in 2008–2010 to 24.2% in 2017–2019 (p<.001). The CD rate in group 10 experienced a slight elevation, in 2008–2010 46.2% were delivered per CD and in 2017–2019 48.8% (p = .553). The overall size of group 10 decreased, contributing 8.9% in 2008–2010 and 8% in 2017–2019 (p<.05) to the overall birthrate. Conclusion The biggest contributors to the CD rate in our hospital remain multiparous women at term with a previous CD. The CD rates, as well as the overall size of this group, keep rising, resulting in a need to establish more effective ways to motivate women with one previous CD towards vaginal birth after caesarean delivery (VBAC). Furthermore, the CD rate in preterm deliveries is increasing and approaching 50%. This illustrates the need to discuss whether CD is the appropriate mode of delivery in half of the preterm infants.
Evolution of the Epigenetic Landscape in Childhood B Acute Lymphoblastic Leukemia and Its Role in Drug Resistance

This study suggests a major role for epigenetic mechanisms in driving clonal evolution in B-ALL and identifies novel pathways associated with drug resistance. Although B-cell acute lymphoblastic leukemia (B-ALL) is the most common malignancy in children and while highly curable, it remains a leading cause of cancer-related mortality. The outgrowth of tumor subclones carrying mutations in genes responsible for resistance to therapy has led to a Darwinian model of clonal selection. Previous work has indicated that alterations in the epigenome might contribute to clonal selection, yet the extent to which the chromatin state is altered under the selective pressures of therapy is unknown. To address this, we performed chromatin immunoprecipitation, gene expression analysis, and enhanced reduced representation bisulfite sequencing on a cohort of paired diagnosis and relapse samples from individual patients who all but one relapsed within 36 months of initial diagnosis. The chromatin state at diagnosis varied widely among patients, while the majority of peaks remained stable between diagnosis and relapse. Yet a significant fraction was either lost or newly gained, with some patients showing few differences and others showing massive changes of the epigenetic state. Evolution of the epigenome was associated with pathways previously linked to therapy resistance as well as novel candidate pathways through alterations in pyrimidine biosynthesis and downregulation of polycomb repressive complex 2 targets. Three novel, relapse-specific superenhancers were shared by a majority of patients including one associated with S100A8, the top upregulated gene seen at relapse in childhood B-ALL. Overall, our results support a role of the epigenome in clonal evolution and uncover new candidate pathways associated with relapse. Significance: This study suggests a major role for epigenetic mechanisms in driving clonal evolution in B-ALL and identifies novel pathways associated with drug resistance.
Expectancy Violation Drives Memory Boost for Stressful Events

Stressful events are often vividly remembered. Although generally adaptive to survival, this emotional-memory enhancement may contribute to stress-related disorders. We tested here whether the enhanced memory for stressful events is due to the expectancy violation evoked by these events. Ninety-four men and women underwent a stressful or control episode. Critically, to manipulate the degree of expectancy violation, we gave participants either detailed or minimal information about the stressor. Although the subjective and hormonal stress responses were comparable in informed and uninformed participants, prior information about the stressor abolished the memory advantage for core features of the stressful event, tested 7 days later. Using functional near-infrared spectroscopy, we further linked the expectancy violation and memory formation under stress to the inferior temporal cortex. These data are the first to show that detailed information about an upcoming stressor and, by implication, a reduced expectancy violation attenuates the memory for stressful events.
AFLP LINKAGE MAP OF AN INTRASPECIFIC CROSS IN CHLAMYS FARRERI

Zhikong scallop, Chlamys farreri, is the most important species for sea-scallop breeding programs in China. The linkage maps of C. farreri were constructed with AFLP markers generated from 21 primer combinations. The polymorphism level of F1 progeny is 62.94%. Of the 783 polymorphic markers detected, 317 segregated in 1:1 ratios and 190 in 3:1 ratios, whereas the other 276 showed segregation distortion ( 0.05). Linkage analysis used the program MAPMAKER/EXP at LOD 2.5 and 0.30. The male map containing 94 markers in 19 linkage groups, was 1511.4 cM in total length and 66.56% in genome coverage. The female map containing 97 markers in 20 linkage groups, was 1610.2 cM in total length and 66.05% in genome coverage. The distribution of AFLP markers is relatively even in chromosomes of male and female map, because only few of clusters were observed.
In Vivo Products Inflammatory Response to Bacterial 12/15-Lipoxygenase Regulates the

The peritoneal macrophage (M (cid:1) ) is the site of greatest 12/15-lipoxygenase (12/15-LOX) expression in the mouse; however, its immunoregulatory role in this tissue has not been explored. Herein, we show that 12/15-LOX is expressed by 95% of resident peritoneal CD11b high cells, with the remaining 5% being 12/15-LOX (cid:2) . 12/15-LOX (cid:3) cells are phenotypically deﬁned by high F4/80, SR-A, and Siglec1 expression, and enhanced IL-10 and G-CSF generation. In contrast, 12/15-LOX (cid:2) cells are a dendritic cell population. Resident peritoneal M (cid:1) numbers were signiﬁcantly increased in 12/15-LOX (cid:2) / (cid:2) mice, suggesting alterations in migratory trafﬁcking or cell differentiation in vivo. In vitro, M (cid:1) from 12/15-LOX (cid:2) / (cid:2) mice exhibit multiple abnormalities in the regulation of cytokine/growth factor production both basally and after stimulation with Staphylococcus epidermidis cell-free supernatant. Resident adherent cells from 12/15-LOX (cid:2) / (cid:2) mice generate more IL-1, IL-3, GM-CSF, and IL-17, but less CCL5/RANTES than do cells from wild-type mice, while Staphylococcus epidermidis cell-free supernatant-elicited 12/15-LOX (cid:2) / (cid:2) adherent cells release less IL-12p40, IL-12p70, and RANTES, but more GM-CSF. This indicates a selective effect of 12/15-LOX on peritoneal cell cytokine production. In acute sterile peritonitis, 12/15-LOX (cid:3) cells and LOX products were cleared, then reappeared during the resolution phase. The peritoneal lavage of 12/15-LOX (cid:2) / (cid:2) mice showed elevated TGF- (cid:4) 1, along with increased immigration of monocytes/M (cid:1) , but decreases in several cytokines including RAN-TES/CCL5, MCP-1/CCL2, G-CSF, IL-12-p40, IL-17, and TNF- (cid:5) . No changes in neutrophil or lymphocyte numbers were seen. In summary, endogenous 12/15-LOX deﬁnes the resident M (cid:6) population and regulates both the recruitment of mono-cytes/M (cid:1) and cytokine response to bacterial products in vivo. The Journal of Immunology, 2008, 181: 6514–6524.
Failure Risk Analysis on Screw Compressor using Failure Mode and Effect Analysis (FMEA) Method

Screw compressor is a compressor that uses impeller media to compress air. This compressor is included in the type of rotary compressor. In the work process, the screw compressor is equipped with several supporting components. The screw compressor consists of 5 components, namely: intake filter, main motor, v-belt, screw compressor, and oil filter. To maintain the performance of the screw compressor, it requires a method that can predict the critical time in screw compressor components and can determine the impact of damage on an operation. In this study, qualitative analysis and quantitative analysis will be conducted to predict and determine the time and effect of screw compressor components failure. The method used in qualitative analysis is Failure Mode and Effect Analysis (FMEA) and Logic Tree Analysis (LTA) methods. While the quantitative analysis uses the reability function (Rt), hazard rate function (ht), and mean time to failure (MTTF) parameters. By using qualitative FMEA analysis, the repair priority order in the screw compressor is the oil filter, and oil filter is C category using LTA analysis, it means if the failure mode does not have an impact on safety or operational plant and only causes relatively small economic losses for repairs. While quantitative methods results recommended treatment time, intake filter must be maintained 25 27 days, oil filter must be maintained 28 29 days, V-belt must be maintained 105 125 days, the main motor must be maintained 330 335 days, and screw must be maintained 292 313 days.
Metabolomics in chronic pain research

Metabolomics deals with the identification and quantification of small molecules (metabolites) in biological samples. As metabolite levels can reflect normal or altered metabolic pathways, their measurement provides information to improve the understanding, diagnosis and management of diseases. Despite its immense potential, metabolomics applications to pain research have been sparse. This paper describes current metabolomics techniques, reviews published human metabolomics pain research and compares successful metabolomics research in other areas of medicine with the goal of highlighting opportunities offered by metabolomics to advance pain medicine.
Neurobehavior of newborn infants exposed prenatally to methadone and identification of a neurobehavioral profile linked to poorer neurodevelopmental outcomes at age 24 months

The abuse of prescription opioids and heroin by women of childbearing age over the past decade has resulted in a five-fold increase in the number of infants born opioid-dependent. Daily opioid substitution treatment with methadone is associated with less maternal illicit opioid use and improved antenatal care. However, research on the neurobehavioral effects of daily prenatal exposure to methadone on the infant is limited. Using the NICU Network Neurobehavioral Scale (NNNS), we compared the neurobehavior at birth of 86 infants born to opioid-dependent mothers receiving methadone treatment (MMT) with 103 infants unexposed to methadone. Generalized linear models, adjusted for covariates, showed methadone exposed infants had significantly poorer attention, regulation, and quality of movement. They were also significantly more excitable, more easily aroused, exhibited more non-optimal reflexes, hypertonicity, and total signs of stress abstinence. Maternal MMT was also associated with more indices of neonatal abstinence, including: CNS, visual, genitourinary (GI), and state. Latent profile analysis of the NNNS summary scores revealed four distinct neurobehavioral profiles with infants characterized by the most disturbed neurobehavior at birth having the poorest clinical outcomes at birth, and poorer cognitive and motor development at 24 months of age.
Clinico‐immunological outcomes of HCV‐cured cryoglobulinemia: Lower relapse rate with interferon‐based than interferon‐free therapy

Sustained virological response (SVR) obtained with interferon (IFN) or with direct‐acting antivirals (DAAs) is commonly followed by response of hepatitis C virus (HCV)‐associated mixed cryoglobulinemia vasculitis (MCV), but relapse of MCV despite SVR has been reported in several patients after DAAs and rarely after IFN. Since relapses could have been overlooked in studies with IFN, we retrospectively compared the outcomes of MCV in SVR patients treated with DAAs (n = 70) or IFN (n = 39) followed‐up, respectively, for 30.5 (range 11‐51) or 48 months. Groups were comparable for demographics and clinics and response rates of MCV were similar (92% and 86%); however, DAA‐treated patients less efficiently reduced cryoglobulins (P = .006) and circulating B‐cell clones (P = .004), and had more frequently relapses of MCV (18% vs 3%, P = .028) and need for rituximab therapy (P = .01). Although largely inferior on an intention‐to‐treat basis, IFN may be superior to DAAs on clinico‐immunological outcomes possibly owing to its antiproliferative activity.
Susceptibility of Protein Methionine Oxidation in Response to Hydrogen Peroxide Treatment–Ex Vivo versus In Vitro: A Computational Insight

Methionine oxidation plays a relevant role in cell signaling. Recently, we built a database containing thousands of proteins identified as sulfoxidation targets. Using this resource, we have now developed a computational approach aimed at characterizing the oxidation of human methionyl residues. We found that proteins oxidized in both cell-free preparations (in vitro) and inside living cells (ex vivo) were enriched in methionines and intrinsically disordered regions. However, proteins oxidized ex vivo tended to be larger and less abundant than those oxidized in vitro. Another distinctive feature was their subcellular localizations. Thus, nuclear and mitochondrial proteins were preferentially oxidized ex vivo but not in vitro. The nodes corresponding with ex vivo and in vitro oxidized proteins in a network based on gene ontology terms showed an assortative mixing suggesting that ex vivo oxidized proteins shared among them molecular functions and biological processes. This was further supported by the observation that proteins from the ex vivo set were co-regulated more often than expected by chance. We also investigated the sequence environment of oxidation sites. Glutamate and aspartate were overrepresented in these environments regardless the group. In contrast, tyrosine, tryptophan and histidine were clearly avoided but only in the environments of the ex vivo sites. A hypothetical mechanism of methionine oxidation accounts for these observations presented.
Functional tests for adults with asthma: validity, reliability, minimal detectable change, and feasibility

Abstract Objective To verify the validity, reliability, learning effect, Minimal Detectable Change (MDC), and feasibility of four functional tests (4-Meter Gait Speed [4MGS], Timed Up-and-Go [TUG], Sit-To-Stand [STS], and Short Physical Performance Battery [SPPB]) for adults with asthma. Methods In this cross-sectional study, fifty-two subjects with stable asthma underwent three sets of different functional tests protocols (4MGS, TUG, STS, SPPB) in a random order by two raters. For validation analysis, tests were compared with a sex-age matched control group without asthma and correlated with the Six Minute Walking Test (6MWT), and peripheral muscle strength, as well as with quality of life and asthma control questionnaires. Intra-rater and inter-rater reliability, MDC, and feasibility were verified. Results Adults with asthma presented worse results than controls in the functional tests, except for SPPB. All functional tests were significantly correlated with 6MWT (0.45 < r < 0.67) and peripheral muscle strength (0.32 < r < 0.63), but not with quality of life and asthma control (0.02 < r < 0.17). The tests presented good to excellent intra-rater Intraclass Correlation Coefficients (ICC ≥ 0.75 for all). In all tests, a considerable learning effect and variability of measurement was observed, therefore, the best of two measurements should be used. MDC ranged from 15 to 31% and all tests were performed in a short time, small space, and without clinical adverse events. Conclusion Different protocols of 4MGS, TUG, STS, and SPPB are valid, reliable, and feasible to assess the functional capacity of adults with asthma. These tests are quick and practical new alternatives for assessing functional capacity in this population.
Health news sharing is reflected in distributed reward-related brain activity

Abstract Neuroimaging has identified individual brain regions, but not yet whole-brain patterns, that correlate with the population impact of health messaging. We used neuroimaging to measure whole-brain responses to health news articles across two studies. Beyond activity in core reward value-related regions (ventral striatum, ventromedial prefrontal cortex), our approach leveraged whole-brain responses to each article, quantifying expression of a distributed pattern meta-analytically associated with reward valuation. The results indicated that expression of this whole-brain pattern was associated with population-level sharing of these articles beyond previously identified brain regions and self-report variables. Further, the efficacy of the meta-analytic pattern was not reducible to patterns within core reward value-related regions but rather depended on larger-scale patterns. Overall, this work shows that a reward-related pattern of whole-brain activity is related to health information sharing, advancing neuroscience models of the mechanisms underlying the spread of health information through a population.
Etude ethnobotanique sur les plantes médicinales spontanées poussant dans le versant nord de l’Atlas d’Azilal (Maroc)

Dans ce manuscrit, l’inventaire ainsi que les usages therapeutiques des plantes medicinales spontanees poussant dans le versant nord de l’Atlas d’Azilal (Maroc) ont ete repertories. 66 especes vegetales spontanees sont utilisees en phytotherapie dont 14 especes ont un usage frequent. Les Gymnospermes ne sont representees que par trois especes, tandis que les Angiospermes sont representees par 63 especes dont 55 sont des dicotyledones et huit sont des monocotyledones. Les familles vegetales, bien representees dans la region exploree, sont les Lamiaceae et les Asteraceae . A la base de l’enquete ethnobotanique, nous avons recense 25 plantes medicinales spontanees destinees au traitement des affections digestives, huit plantes pour traiter les troubles uro-genitaux, cinq especes sont connues pour leurs effets sur le systeme nerveux, 14 plantes sont utilisees contre les affections metaboliques, 11 plantes pour soigner les maladies de l’appareil respiratoire et 26 plantes sont employees pour les soins la peau et les cheveux.
BAX 335 hemophilia B gene therapy clinical trial results - potential impact of CpG sequences on gene expression.

Gene therapy has the potential to maintain therapeutic blood clotting factor IX (FIX) levels in patients with hemophilia B by delivering a functional human F9 gene into liver cells. This phase 1/2, open-label dose-escalation study investigated BAX 335 (AskBio009, AAV8.sc-TTR-FIXR338Lopt), an adeno-associated virus (AAV) serotype 8-based FIX Padua gene therapy, in patients with hemophilia B. This report focuses on 12-month interim analyses of safety, pharmacokinetics, effects on FIX activity, and immune responses for dosed participants. Eight adult males (\aged 20-69 years; range FIX activity, 0.5%-2.0%) received 1 of 3 BAX 335 IV doses: 2.0 × 1011; 1.0 × 1012; or 3.0 × 1012 vector genomes/kg. Three (37.5%) participants had 4 serious adverse events (SAEs), all considered unrelated to BAX 335. No SAE led to death. No clinical thrombosis, inhibitors, or other FIX Padua-directed immunity were reported. FIX expression was measurable in 7 of 8 participants; peak FIX activity displayed dose dependence (32.0%-58.5% in cohort 3). One participant achieved sustained therapeutic FIX activity of ~20%, without bleeding or replacement therapy, for 4 years; in others, FIX activity was not sustained beyond 5-11 weeks. In contrast to some previous studies, corticosteroid treatment did not stabilize FIX activity loss. We hypothesize that the loss of transgene expression could have been caused by stimulation of innate immune responses including CpG oligodeoxynucleotides introduced into the BAX 335 coding sequence by codon optimization. Registered at www.clinicaltrials. gov as NCT01687608.