Miracle-dz/UniFAR-FTUs huggingface dataset:
ASSOCIATION BETWEEN DRINKING WATER SOURCES AND DIARRHEA WITH MALNUTRITION AMONG KINDERGARTEN'S CHILDREN IN BAGHDAD CITY, IRAQ

ABSTRACT Malnutrition is associated with childhood diseases such as diarrhea or other severe illness. Children who suffer from repeated episodes of diarrhea are more likely to suffer from malnutrition. In addition, the source of drinking water is a very important factor affecting the nutritional status. The objective of this study was to find out the prevalence of malnutrition condition among children aged 3 to 5 years old and the factors associated with it. A cross sectional study using simple random sampling was conducted among 220 children aged 3 to 5 years old from four kindergartens in Baghdad city, Iraq. Nutritional assessment was carried out using the indicator weight-for-age z-score based on World Health Organization cut off points. Acute malnutrition was found in 18.2 % of the respondents. In this study 65 % of the respondents used tap water to drink as compared to 35 % who used sterilized bottles as source of drinking water. The environmental factors mainly the source of drinking water was significantly associated with child nutritional status (p=0.034). The prevalence of diarrhea and admissions to the hospital due to diarrhea were also significantly associated with malnutrition (p < 0.01, p <0.01) respectively. In conclusion, the effect of diarrhea on child nutritional status is very important and till now considered the major cause of child malnutrition. Shortage of safe drinking water in Iraq can lead to an increase in diarrhea cases and eventually leading to child malnutrition.
The effects of nursing intervention on the postoperative quality of life and early compliance of patients with cervical cancer

Objective: This study aimed to analyze the effects of nursing intervention on the postoperative quality of life and early compliance of patients with cervical cancer. Methods: A total of 96 patients undergoing cervical cancer surgery in our hospital from February 2017 to November 2018 were recruited for the study, in which 48 randomly-selected patients were nursed comprehensively as an observation group, and the other 48 patients were nursed routinely as a control group. The patient compliance in the two groups after the nursing was evaluated according to the eight-item Morisky Medication Adherence Scale (MMAS-8), and their quality of life was evaluated based on the MOS 36-Item Short-Form Health Survey (SF-36). The two groups were compared in terms of nursing satisfaction. A multivariate logistic regression analysis was adopted to analyze the risk factors for postoperative bladder dysfunction, and a receiver operating characteristic (ROC) curve was used to analyze the diagnostic values of age, surgical approach, and nursing pattern for postoperative bladder dysfunction. Results: The observation group got significantly higher MMAS-8 and SF-36 scores than the control group and showed significantly higher total nursing satisfaction than the control group (all P<0.05). A multivariate logistic regression analysis revealed that age, surgical approach, and nursing pattern were independent risk factors for postoperative bladder dysfunction. The ROC curves showed that the areas under the curve (AUC) for age, surgical approach, and nursing pattern were 0.669 (95% CI: 0.552-0.786), 0.635 (95% CI: 0.513-0.756), and 0.612 (95% CI: 0.513-0.756), respectively. Conclusion: A comprehensive nursing pattern can effectively improve the postoperative quality of life and compliance of patients with cervical cancer. Age, surgical approach, and nursing pattern are independent risk factors for postoperative bladder dysfunction, and we can predict the postoperative bladder dysfunction of patients based on those factors.
A phenomenological exploration of therapeutic experiences of the transpersonal.

This study explores therapists' experience of participation with the transpersonal in the therapeutic encounter, and considers this in the light of implications for therapy training. It incorporates a: quantitative study, based upon questionnaire results; qualitative study, based upon a phenomenological analysis of interviews with therapists; review of relevant literature; discussion of the study's integrated findings; conclusion; and discussion of how the study is understood as a whole, which considers the study's validity, implications for future research and personal reflections on conducting the study. Results indicate that incorporating spirituality into a therapeutic approach is important in terms of. eradicating the potential for damage to both clients' and therapists' relationships with the transpersonal; accommodating the possibility of participation with transpersonal processes, for in this lies greater scope for healing; and the accommodation of a wider range of client experience that includes the spiritual dimension. Therapists indicate that it is context that makes therapy transpersonal, and that this is shaped by therapists' individual transpersonal interests and their assimilation into the therapist's way of being. Key to what therapists bring are their encounters with and understandings of the nature of the transpersonal. These are multifarious, highlighting the need for training to avoid reducing transpersonal participation to a prescriptive set of skills. Training plays an important role in enhancing transpersonal interests, and if healing is the goal of therapy then trainings that foster humility, sincerity, honesty of intention, a reduction in self-centredness, the ability to wait, to `let go' and ̀ let be' are required. The findings highlight an understanding of spirituality that is based on being-in-theworld and interconnectedness, and that challenges self-centredness and self-interest. Since most therapists, possibly unknowingly, already participate in transpersonal processes, the long overdue need for open acknowledgement, dialogue and discussion about these significant experiences, events and processes that have been ignored and pathologised for too long, requires urgent attention.
May the Force Not Be With You During Culture: Eliminating Mechano-Associated Feedback During Culture Preserves Cultured Atrial and Pacemaker Cell Functions

Cultured cardiomyocytes have been shown to possess significant potential as a model for characterization of mechano-Ca2+, mechano-electric, and mechano-metabolic feedbacks in the heart. However, the majority of cultured cardiomyocytes exhibit impaired electrical, mechanical, biochemical, and metabolic functions. More specifically, the cells do not beat spontaneously (pacemaker cells) or beat at a rate far lower than their physiological counterparts and self-oscillate (atrial and ventricular cells) in culture. Thus, efforts are being invested in ensuring that cultured cardiomyocytes maintain the shape and function of freshly isolated cells. Elimination of contraction during culture has been shown to preserve the mechano-Ca2+, mechano-electric, and mechano-metabolic feedback loops of cultured cells. This review focuses on pacemaker cells, which reside in the sinoatrial node (SAN) and generate regular heartbeat through the initiation of the heart’s electrical, metabolic, and biochemical activities. In parallel, it places emphasis on atrial cells, which are responsible for bridging the electrical conductance from the SAN to the ventricle. The review provides a summary of the main mechanisms responsible for mechano-electrical, Ca2+, and metabolic feedback in pacemaker and atrial cells and of culture methods existing for both cell types. The work concludes with an explanation of how the elimination of mechano-electrical, mechano-Ca2+, and mechano-metabolic feedbacks during culture results in sustained cultured cell function.
Nitrogen Depletion Blocks Growth Stimulation Driven by the Expression of Nitric Oxide Synthase in Tobacco

Nitric oxide (NO) is a messenger molecule widespread studied in plant physiology. Latter evidence supports the lack of a NO-producing system involving a NO synthase (NOS) activity in higher plants. However, a NOS gene from the unicellular marine alga Ostreococcus tauri (OtNOS) was characterized in recent years. OtNOS is a genuine NOS, with similar spectroscopic fingerprints to mammalian NOSs and high NO producing capacity. We are interested in investigating whether OtNOS activity alters nitrogen metabolism and nitrogen availability, thus improving growth promotion conditions in tobacco. Tobacco plants were transformed with OtNOS under the constitutive CaMV 35S promoter. Transgenic tobacco plants expressing OtNOS accumulated higher NO levels compared to siblings transformed with the empty vector, and displayed accelerated growth in different media containing sufficient nitrogen availability. Under conditions of nitrogen scarcity, the growth promoting effect of the OtNOS expression is diluted in terms of total leaf area, protein content and seed production. It is proposed that OtNOS might possess a plant growth promoting effect through facilitating N remobilization and nitrate assimilation with potential to improve crop plants performance.
Why normalized gain should continue to be used in analyzing preinstruction and postinstruction scores on concept inventories

Recently, Nissen et al. argued in this journal for the use of Cohen’s d, in place of the more commonly used normalized gain, in the analysis of preinstruction and postinstruction scores on concept inventories used to measure the effectiveness of instruction. Their reason for advocating such a change is that they say normalized gains are “prescore biased. ” We provide five examples, including one cited by Nissen, that show no prescore bias when data are carefully analyzed, demonstrating that the problem with their analysis is omitted variable bias. We show that Cohen’s d is less informative than normalized gain when used as a single parameter measure of teaching effectiveness, even though, as Nissen points out, d is more widely used in other fields. We believe that physics education researchers should continue to use normalized gain to assess educational effectiveness of pedagogy. However, because different student populations can have significantly different responses to the same pedagogy, in any interpretation of normalized gain, it is important to consider a measure of the abilities of the students. In analyzing normalized gains for the Force Concept Inventory (FCI), average scores on either Lawson’s Test of Scientific Reasoning Ability or the SAT should be considered, because these scores are strongly correlated with normalized gain, indicating student abilities may have a greater impact on the gains achieved in a class than the specific pedagogy used.
HSD17B4, ACAA1, and PXMP4 in Peroxisome Pathway Are Down-Regulated and Have Clinical Significance in Non-small Cell Lung Cancer

To explore the potential functions and clinical significances of peroxisomes during lung cancer development and progression, we investigated the expressional profiles of peroxisome pathway genes and their correlations with clinical features in non-small cell lung cancer (NSCLC). The RNA-seq data of NSCLC including lung squamous carcinoma (LUSC) and lung adenocarcinoma (LUAD) patients with their clinical information were downloaded from The Cancer Genome Atlas (TCGA). Gene expression comparisons between tumor and normal samples were performed with edgeR package in R software and the results of the 83 peroxisome pathway genes were extracted. Through Venn diagram analysis, 38 common differentially expressed peroxisome pathway genes (C-DEPGs) in NSCLC were identified. Principal components analysis (PCA) was performed and the 38 C-DEPGs could discriminate NSCLC tumors from the non-tumor controls well. Through Kaplan-Meier survival and Cox regression analyses, 11 of the C-DEPGs were shown to have prognostic effects on NSCLC overall survival (OS) and were considered as key C-DEPGs (K-DEPGs). Through Oncomine, Human Protein Atlas (HPA) and the Clinical Proteomic Tumor Analysis Consortium (CPTAC), three K-DEPGs (HSD17B4, ACAA1, and PXMP4) were confirmed to be down-regulated in NSCLC at both mRNA and protein level. Their dy-regulation mechanisms were revealed through their correlations with their copy number variations and methylation status. Their potential functions in NSCLC were explored through their NSCLC-specific co-expression network analysis, their correlations with immune infiltrations, immunomodulator gene expressions, MKI67 expression and their associations with anti-cancer drug sensitivity. Our findings suggested that HSD17B4, ACAA1, and PXMP4 might be new markers for NSCLC diagnosis and prognosis and might provide new clues for NSCLC treatment.
The Chloroplast Ribonucleoprotein CP33B Quantitatively Binds the psbA mRNA

Chloroplast RNAs are stabilized and processed by a multitude of nuclear-encoded RNA binding proteins, often in response to external stimuli like light and temperature. A particularly interesting RNA based regulation occurs with the psbA mRNA, which shows light-dependent translation. Recently, the chloroplast ribonucleoprotein CP33B was identified as a ligand of the psbA mRNA. We here characterized the interaction of CP33B with chloroplast RNAs in greater detail using a combination of RIP-chip, quantitative dot-blot, and RNA-Bind-n-Seq experiments. We demonstrate that CP33B prefers psbA over all other chloroplast RNAs and associates with vast majority of the psbA transcript pool. The RNA sequence target motif determined in vitro does not fully explain CP33B’s preference for psbA, suggesting that there are other determinants of specificity in vivo.
Host membrane lipids are trafficked to membranes of intravacuolar bacterium Ehrlichia chaffeensis

Significance Biological membranes are essential for life. Although bacteria and eukaryotic cells have evolved to produce membranes of different compositions, several bacterial pathogens can hijack and utilize host-synthesized membrane lipids. Here, we show that an obligatory intracellular pathogen, Ehrlichia chaffeensis, deficient in biosynthesis of cholesterol and some glycerophospholipids, actively acquires host-derived membrane components within membrane-bound inclusions (vacuoles). The trafficking of host membrane components to Ehrlichia and membrane-bound inclusions appears to occur via endocytosis and autophagy induced by a bacteria-secreted protein. Numerous intraluminal vesicles were found in Ehrlichia inclusions that may function as a membrane reserve for rapid proliferation of Ehrlichia. Our findings provide insights into host membrane assimilation by an intracellular pathogen, which can be exploited for antibacterial therapy. Ehrlichia chaffeensis, a cholesterol-rich and cholesterol-dependent obligate intracellular bacterium, partially lacks genes for glycerophospholipid biosynthesis. We found here that E. chaffeensis is dependent on host glycerolipid biosynthesis, as an inhibitor of host long-chain acyl CoA synthetases, key enzymes for glycerolipid biosynthesis, significantly reduced bacterial proliferation. E. chaffeensis cannot synthesize phosphatidylcholine or cholesterol but encodes enzymes for phosphatidylethanolamine (PE) biosynthesis; however, exogenous NBD-phosphatidylcholine, Bodipy-PE, and TopFluor-cholesterol were rapidly trafficked to ehrlichiae in infected cells. DiI (3,3′-dioctadecylindocarbocyanine)-prelabeled host-cell membranes were unidirectionally trafficked to Ehrlichia inclusion and bacterial membranes, but DiI-prelabeled Ehrlichia membranes were not trafficked to host-cell membranes. The trafficking of host-cell membranes to Ehrlichia inclusions was dependent on both host endocytic and autophagic pathways, and bacterial protein synthesis, as the respective inhibitors blocked both infection and trafficking of DiI-labeled host membranes to Ehrlichia. In addition, DiI-labeled host-cell membranes were trafficked to autophagosomes induced by the E. chaffeensis type IV secretion system effector Etf-1, which traffic to and fuse with Ehrlichia inclusions. Cryosections of infected cells revealed numerous membranous vesicles inside inclusions, as well as multivesicular bodies docked on the inclusion surface, both of which were immunogold-labeled by a GFP-tagged 2×FYVE protein that binds to phosphatidylinositol 3-phosphate. Focused ion-beam scanning electron microscopy of infected cells validated numerous membranous structures inside bacteria-containing inclusions. Our results support the notion that Ehrlichia inclusions are amphisomes formed through fusion of early endosomes, multivesicular bodies, and early autophagosomes induced by Etf-1, and they provide host-cell glycerophospholipids and cholesterol that are necessary for bacterial proliferation.
Sleep Duration, Bedtime, and Myopia Progression in a 4-Year Follow-up of Chinese Children: The Anyang Childhood Eye Study

Purpose To investigate the relationship between sleep duration and bedtime with myopia progression and axial elongation during a 4-year follow-up in primary school children. Methods This study included 1887 children (aged 7.09 ± 0.41 years) who had cycloplegic refractions data at baseline and a fourth visit and 2209 children (aged 7.10 ± 0.41 years) for axial length. All children underwent comprehensive ophthalmologic examinations, including cycloplegic refraction and ocular biometry, and standardized questionnaires, including average night-time sleep duration (h/d) and bedtime (time to bed). Myopia was defined as spherical equivalent < –0.5 diopters. Results At the last follow-up, the mean myopia progression and axial elongation for all children were –1.89 ± 1.28 diopters and 1.22 ± 0.57 mm. After stratifying the sleep duration into tertile groups, myopia progression and axial elongation were slower in children with highest sleep duration tertile (P = 0.04 and P =0.014) in girls but not in boys, compared with the middle sleep duration tertile. However, after adjusting for potential confounders, no significant association was found for sleep duration with myopia progression and axial elongation for the children (P = 0.255 and P = 0.068), and the association with axial elongation was only of borderline significance in girls (P = 0.045). The bedtime was not associated with myopia progression and axial elongation in the regression analyses (P = 0.538; P = 0.801). Conclusions These results show that there was no significant association between sleep duration and bedtime with myopia progression and axial elongation among children. The findings in girls might be related to the earlier onset of puberty.
Erectile dysfunction and cardiovascular risk: a review of current findings

ABSTRACT Introduction: A large body of evidence has clearly documented that erectile dysfunction (ED) represents not only a complication of cardiovascular (CV) diseases (CVD) but often an early sign of forthcoming CVD. Areas covered: All the available data from meta-analyses evaluating the association between ED and CV risk were collected and discussed. Similarly, all available meta-analyses investigating the significance of ED as a possible early marker for major adverse cardiovascular events (MACE) were analyzed. In addition, data originally obtained in a Florence cohort, dealing with a large series of patients seeking medical care for sexual dysfunction, will be also reported. Expert opinion: Available evidence indicates that ED represents a risk factor of CV mortality and morbidity. Not only conventional CV risk factors but also unconventional ones, derived from a perturbation of the relational and intrapsychic domains of ED, might play a possible role in CV risk stratification of ED subjects. Finally, penile doppler ultrasound can give important information on CV risk, especially in younger and low risk subjects. The presence of ED should become an opportunity – for the patient and for the physician – to screen for the presence of comorbidities improving not only sexual health but, more importantly, men’s overall health.
Magnetoencephalography resting state connectivity patterns as indicatives of surgical outcome in epilepsy patients

Objective. Focal epilepsy is a disorder affecting several brain networks; however, epilepsy surgery usually targets a restricted region, the so-called epileptic focus. There is a growing interest in embedding resting state (RS) connectivity analysis into pre-surgical workup. Approach. In this retrospective study, we analyzed Magnetoencephalography (MEG) long-range RS functional connectivity patterns in patients with drug-resistant focal epilepsy. MEG recorded prior to surgery from seven seizure-free (Engel Ia) and five non seizure-free (Engel III or IV) patients were analyzed (minimum 2-years post-surgical follow-up). MEG segments without any detectable epileptic activity were source localized using wavelet-based Maximum Entropy on the Mean method. Amplitude envelope correlation in the theta (4–8 Hz), alpha (8–13 Hz), and beta (13–26 Hz) bands were used for assessing connectivity. Main results. For seizure-free patients, we found an isolated epileptic network characterized by weaker connections between the brain region where interictal epileptic discharges (IED) are generated and the rest of the cortex, when compared to connectivity between the corresponding contralateral homologous region and the rest of the cortex. Contrarily, non seizure-free patients exhibited a widespread RS epileptic network characterized by stronger connectivity between the IED generator and the rest of the cortex, in comparison to the contralateral region and the cortex. Differences between the two seizure outcome groups concerned mainly distant long-range connections and were found in the alpha-band. Significance. Importantly, these connectivity patterns suggest specific mechanisms describing the underlying organization of the epileptic network and were detectable at the individual patient level, supporting the prospect use of MEG connectivity patterns in epilepsy to predict post-surgical seizure outcome.
Food Sources of Dietary Potassium in the Adult Japanese Population: The International Study of Macro-/Micronutrients and Blood Pressure (INTERMAP)

A lower-than-recommended potassium intake is a well-established risk factor for increased blood pressure. Although the Japanese diet is associated with higher sodium intake and lower potassium intake, few studies have examined the source foods quantitatively. Studies on dietary patterns in association with potassium intake will be useful to provide dietary advice to increase potassium intake. Twenty-four-hour (hr) dietary recall data and 24-hr urinary potassium excretion data from Japanese participants (574 men and 571 women) in the International Study of Macro/Micronutrients and Blood Pressure (INTERMAP) were used to calculate food sources of potassium and compare food consumption patterns among quartiles of participants categorized according to 24-hr urinary potassium excretion per unit of body weight (UK/BW). The average potassium intake was 2791 mg/day per participant, and the major sources were vegetables and fruits (1262 mg/day), fish (333 mg/day), coffee and tea (206 mg/day), and milk and dairy products (200 mg/day). Participants in the higher UK/BW quartile consumed significantly more vegetables and fruits, fish, and milk and dairy products, and ate less rice and noodles. Conclusion: Advice to increase the intake of vegetables and fruits, fish, and milk may be useful to increase potassium intake in Japan.