Miracle-dz/UniFAR-FTUs huggingface dataset:
Enteric methane, lactation performances, digestibility, and metabolism of nitrogen and energy of Holsteins and Jerseys fed 2 levels of forage fiber from alfalfa silage or corn silage.

Our objective was to determine the effects of replacing alfalfa silage (AS) neutral detergent fiber (NDF) with corn silage (CS) NDF at 2 levels of forage NDF (FNDF) on enteric methane (CH4), lactation performance, ruminal fluid characteristics, digestibility, and metabolism of N and energy in Holstein and Jersey cows. Twelve Holstein and 12 Jersey cows (all primiparous and mid-lactation) were used in a triplicated split-plot 4 × 4 Latin square experiment, where breed and diet formed the main and subplots, respectively. The 4 iso-nitrogenous and iso-starch dietary treatments were arranged as a 2 × 2 factorial with 2 levels of FNDF [19 (low FNDF, LF) and 24% (high FNDF, HF) of dry matter] and 2 sources of FNDF (70:30 and 30:70 ratio of AS NDF to CS NDF). Soyhull (non-forge NDF) and corn grain were respectively used to keep dietary NDF and starch content similar across diets. Total collection of feces and urine over 3 d was performed on 8 cows (1 Latin square from each breed). The difference in dry matter intake (DMI) between Holsteins and Jerseys was greater when fed AS than CS. Compared with Jerseys, Holstein cows had greater body weight (48%), DMI (34%), fat- and protein-corrected milk (FPCM; 31%) and CH4 production (22%; 471 vs. 385 g/d). However, breed did not affect CH4 intensity (g/kg of FPCM) or yield (g/kg of DMI), nutrient digestibility, and N partitioning. Compared with HF, LF-fed cows had greater DMI (10%), N intake (8%), and FPCM (5%), but they were 5% less efficient (both FPCM/DMI and milk N/intake N). Compared with HF, LF-fed cows excreted 11 and 17% less urinary N (g/d and % of N intake, respectively). In spite of lower (2.5%) acetate and higher (10%) propionate (mol/100 mol ruminal volatile fatty acids) LF-fed cows had greater (6%) CH4 production (g/d) than did HF-fed cows, most likely due to increased DMI, as affected mainly by the soyhulls. Compared with AS, CS-fed cows had greater DMI (7%) and FPCM (4%), but they were less efficient (5%), and CH4 yield (g/kg of DMI) was reduced by 8%. In addition, per unit of gross energy intake, CS-fed cows lost less urinary energy (15%) and CH energy (11%) than did AS-fed cows. We concluded that, in contrast to level and source of FNDF, breed did not affect digestive and metabolic efficiencies, and, furthermore, neither breed nor dietary treatments affected CH4 intensity. The tradeoff between CH4 and N losses may have implications in future studies assessing the environmental effects of milk production when approached from a whole-farm perspective.
α‐Synuclein induces unfolded protein response via distinct signaling pathway independent of er‐membrane kinases

Abastract Parkinson's disease (PD) is a neurodegenerative disease caused by selective degeneration of dopaminergic neurons in the substantia nigra. Mutations in α‐synuclein have been causally linked to the pathogenesis of hereditary PD. In addition, it is a major component of Lewy body found in the brains of sporadic cases as well. In the present study, we examined whether overexpression of wild type or PD‐related mutant α‐synuclein induces unfolded protein response (UPR) and triggers the known signaling pathway of the resulting endoplasmic reticulum (ER) stress in SH‐SY5Y cells. Overexpression of wild type, A30P, and A53T α‐synuclein all induced XBP‐1 mRNA splicing, one of the late stage UPR events. However, activation of ER membrane kinases and upregulation of ER or cytoplsmic chaperones were not detected when α‐synuclein was overexpressed. However, basal level of cytoplsmic calcium was elevated in α‐synuclein‐expressing cells. Our observation suggests that overexpression of α‐synuclein induces UPR independent of the known ER membrane kinase‐mediated signaling pathway and induces ER stress by disturbing calcium homeostasis.
Seleção simultânea para porte reduzido e alta produção de látex em seringueira

Goals of this work were to evaluate genetic variability and potential of successful simultaneous selection for short stature and high rubber yield, considering relational variables: rubber yield per height unit (PBA) and rubber yield per plant basal area (PBB), both as new traits in rubber tree genetic improvement. Selection based on these variables allows identification of new genetic material suitable for high density plantations. Twenty-two half-sib progenies of half-sibs were established in Votuporanga. The trial was carried out with 22 treatments with five replications each and 10 plants per plot, planted in single lines at density of 3 x 2m. Plots were randomly distributed. At three year old age, progenies were evaluated and showed that a combination of those two traits increased genetic variability available for selection. Results also demonstrated that it is possible to have an efficient genetic improvement for simultaneously increasing rubber yield while reducing plant height. Therefore, it is possible to improve these two traits (PB and PBB) simultaneously, without losses on the main traits (PB). High correlations between PBB and PBA (0.842) and between PBB and height (-0.578) show that selection based on PBB will lead to height reduction. The type of selection proved itself efficient for increasing rubber yield while substantially reducing plant height and less reducing trunk perimeter. All these effects are desirable in a rubber tree genetic improvement program aiming at selection of genotypes suitable for higher density plantations.
Biodiversity Hotspots in the Mediterranean Basin: Setting Global Conservation Priorities

Due to the current species extinction crisis, there is an urgent need to identify the most threatened areas of exceptionally high biodiversity and rates of endemism (i.e., "hotspots"; Mittermeier et al. 1998; Myers 1988; Reid 1998). Conservation strategies represent a crucial issue in the mediterranean biome because this area, which represents only 2% of the world's surface, houses 20% of the world's total floristic richness (Medail & Quezel 1997). Myers initially (1988, 1990) defined 14 hotspots in the tropical biome and four in mediterranean bioclimates (southwestern Australia, Cape Region of South Africa, California, and part of Chile). Like the four other mediterranean areas, the Mediterranean Basin is one of the world's major centers for plant diversity, where 10% of the world's higher plants can be found in an area representing only 1.6% of the Earth's surface (Medail & Quezel 1997). The prominent role played by these areas as reservoirs for plant biodiversity has been emphasized by Myers (1990). He hesitated, however, to group the whole Mediterranean Basin into one single hotspot because it covers such a large surface area, and insufficient data were available for certain regions. In this context, Medail and Quezel (1997) performed a global survey of plant richness and endemism to more precisely define hotspots in the Mediterranean Basin; they identified 10 hotspots. Three main approaches, however, have been taken in recent studies performed by international conservation organizations to define priority conservation areas in the Mediterranean Basin.
Improved osteogenic differentiation of human amniotic mesenchymal stem cells on gradient nanostructured Ti surface.

Titanium (Ti) and Ti-based alloys are widely used in the manufacture of dental and orthopedic implants. But how to improve their osteogenic differentiation ability is still a key issue to be resolved. In this study, gradient nanostructured surface (GNS) samples were prepared by surface mechanical grinding treatment (SMAT), and coarse-grained (CG) samples were obtained by recrystallization annealing, making sure that the two kinds of specimens had similar roughness. Then human amniotic mesenchymal stem cells (hAMSCs) were co-cultured with the two kinds of Ti to investigate the material effects on the cellular functions. The results demonstrated that the grains with size ~56 nm were formed on the surface of the GNS Ti, and the grain size gradually increases from the sample surface to interior. Compared to the CG samples, the GNS ones could make the adhesion effect of the hAMSCs better, and promote the cell proliferation and osteogenic differentiation more significantly, the preliminary mechanism of which might be due to their specific nanostructure, the thicker oxide layer formed on their surface and the enhanced hardness. Our results indicated that the gradient nanostructured Ti materials could enhance both osteogenic differentiation and mechanical properties, which may possess broader applications in bone tissue engineering and clinical implanting. This article is protected by copyright. All rights reserved.
New Records of Benthic Marine Algae from Mauritius (Indian Ocean)

This report contains nineteen new records of marine algae from Mauritius (Indian Ocean). Data concerning ecological distribution and biogeography of the species is presented, with some descriptions and figures of the most interesting plants: Chondria armata Okamura, Chondria dangeardii Dawson, Eupogodon pilosus (Weber-van Bosse) Silva, Tolypiocladia calodictyon (Harvey ex Kützing) Silva, and Ulva reticulata Forsskäl.
Contributions to a chromosome atlas of the New Zealand flora—37. Miscellaneous families

Abstract Documented chromosome numbers are reported for 156 taxa (117 species, 3 subspecies, 8 varieties, 3 forma, 1 hybrid, and 24 of uncertain rank) from 24 families (54 genera) of angiosperms. Of these, 122 are for previously uncounted New Zealand taxa. Many of the counts are from poorly investigated families and genera, or from nationally uncommon and threatened, or newly described plants. In the majority of genera the numbers follow established patterns. Counts have now been obtained for all the New Zealand representatives of the Arecaceae and Urticaceae.
Recent developments in the systematics of the Gigartinaceae (Gigartinales, Rhodophyta) based on rbcL sequence analysis and morphological evidence

The phylogenetic systematics of the Gigartinaceae is discussed for seven genera and three undescribed generic lineages and 65 taxa representing 62 species based on an analysis of rbcL sequences and morphological evidence. An examination of rbcL trees resulting from analyses of these taxa identifies seven lineages: (i) ‘Gigartina’ alveata; (ii) Rhodoglossum/Gigartina; (iii) Chondracanthus; (iv) Ostiophyllum; (v) Sarcothalia; (vi) ‘Gigartina’ skottsbergii; and (vii) a large clade containing Iridaea/‘Sarcothalia’, Mazzaella and Chondrus. These lineages and Chondrus are strongly supported; however, two groups, Iridaea/‘Sarcothalia’ and Mazzaella, receive no bootstrap support. The morphology of the female reproductive system is investigated with the aid of computer‐generated, color‐coded tracings of photographs of cystocarps seen in cross section at different developmental stages. Seven basic cystocarp types were found which corresponded to species groups seen in rbcL trees. These were: (i) a ‘Gigartina’ alveata group in which the carposporangia‐bearing filaments develop apomictically from gametophytic cells; (ii) a Rhodoglossum/Gigartina group in which gonimoblast filaments penetrate the surrounding envelope fusing progessively with envelope cells; (iii) a Chondracanthus group in which gonimoblast filaments penetrate the envelope but fuse with envelope cells only at late developmental stages; (iv) a Sarcothalia group in which the gonimoblast filaments displace an envelope composed mainly of secondary gametophytic filaments and link to envelope cells by terminal tubular gonimoblast cells; (v) an Iridaea group similar to the Sarcothalia group, but with an envelope composed of a mixture of medullary cells and secondary gametophytic filaments; (vi) a Mazzaella group that lacks a true envelope and in which gonimoblast filaments connect to modified gametophytic cells by means of terminal tubular cells; (vii) a Chondrus group in which gonimoblast filaments penetrate the medulla and link to modified medullary cells by means of conjunctor cells forming secondary pit connections. The further separation of these groups into genera is based largely on tetrasporangial characters.
Similar Clinical Outcomes of Early and Late Anti-TNF Initiation for Ulcerative Colitis: A Nationwide Population-Based Study

Purpose The optimal timing of anti-tumor necrosis factor (anti-TNF) initiation in patients with ulcerative colitis (UC) remains unclear. Very little is known about the clinical outcomes after the early versus late initiation of anti-TNF therapy, especially in Asian UC patients. Here we aimed to assess whether earlier anti-TNF treatment initiation results in favorable clinical outcomes in Korean UC patients. Materials and Methods Using the Korean National Health Insurance claims database, we studied patients who were diagnosed with UC and received anti-TNF therapy for more than 6 months between 2010 and 2016. Using a Cox proportional hazard model, clinical outcomes including colectomy, UC-related emergency room (ER) visits, UC-related hospitalizations, and the need for corticosteroids were compared between early (≤2 years of diagnosis) and late (>2 years of diagnosis) initiators of anti-TNF therapy. Results Among 17167 UC patients, 698 patients who received anti-TNF therapy for more than 6 months were included (420 infliximab, 242 adalimumab, and 36 golimumab). Of the 698 patients, 299 (42.8%) initiated anti-TNF therapy within 2 years of diagnosis. There were no significant differences in the risk of colectomy [adjusted hazard ratio (aHR), 0.41; 95% confidence interval (CI), 0.04–3.90], ER visits (aHR, 0.98; 95% CI, 0.50–1.92), hospitalization (aHR, 0.76; 95% CI, 0.57–1.01), and corticosteroid use (aHR, 1.04; 95% CI, 0.71–1.50) between early and late initiators of anti-TNF therapy. Conclusion Patients receiving early anti-TNF therapy had similar clinical outcomes to those of late initiators, suggesting that early anti-TNF therapy initiation offers little benefit in patients with UC.
The WWOX gene in brain development and pathology

Shortly after its discovery in 2000, WWOX was hailed as a tumor suppressor gene. In subsequent years of research, this function was confirmed indisputably. Majority of tumors show high rate of loss of heterozygosity and decreased expression of WWOX. Nevertheless, over the years, the range of its known functions, at the cellular, organ and system levels, has expanded to include metabolism and endocrine system control and CNS differentiation and functioning. Despite of its function as a tumor suppressor gene, WWOX genetic alternations were found in a number of metabolic and neural diseases. A lack of WWOX protein as a consequence of germline mutations results in brain development disturbances and malfunctions. Impact statement WW domain-containing oxidoreductase encoded by the WWOX gene is a transcription regulator and a key player in a number of cellular and biological processes such as tumor suppression, cell proliferation, apoptosis induction, steroid metabolism, and central nervous system development. This review provides a comprehensive summary of currently known roles and discusses the importance of WWOX gene for CNS development and functioning.
Morphological Plasticity by Crop Plants and Their Potassium Use Efficiency

Abstract A decrease in the use of fertilizers and a decrease in soil fertility direct the research focus towards the ability of the crops to adapt to low potassium (K) conditions and to their ability to utilize sparingly soluble K sources. Pea (Pisum sativum), red clover (Trifolium pratense), lucerne (Medicago sativa L.), barley (Hordium vulgare), rye (Secale cerale), perennial ryegrass (Lolium perenne L.), and oilseed rape (Brassica napus oliefera) were grown in thin layers of soils to study their root morphological plasticity and ability to utilize different K pools at 26 and 60 mg exchangeable K kg−1 soil. The legumes (pea, red clover, lucerne) accumulated larger amounts of nitrogen but lower amounts of K than rye, ryegrass, barley, and oilseed rape. The differences in K accumulation correlated with root hair length (R 2 = 0.50). Rye had an outstanding root surface that in total as well as per unit root dry matter was twice that of the other crops. The ranking in decreasing order was rye, ryegrass, oilseed rape, lucerne, barley, pea, and red clover. This ranking was unaffected (P > 0.05) by the initial soil K content although the root surface in all cases increased when grown in soil low on K compared to soils with a better K supply. This increase was achieved by increasing root hair length as root hair density was similar (P > 0.05) for all crops and soils. Efficient use of sparingly soluble K pools was important for all investigated crops. In conclusion, crops modify their root hair length as response to low K conditions and thereby maintain the uptake from sparingly soluble K sources.
Malignant gastric outlet obstruction: Which is the best therapeutic option?

Malignant gastric outlet obstruction (MGOO) is a clinical condition characterized by the mechanical obstruction of the pylorus or the duodenum due to tumor compression/infiltration, with consequent reduction or impossibility of an adequate oral intake. MGOO is mainly secondary to advanced pancreatic or gastric cancers, and significantly impacts on patients’ survival and quality of life. Patients suffering from this condition often present with intractable vomiting and severe malnutrition, which further compromise therapeutic chances. Currently, palliative strategies are based primarily on surgical gastrojejunostomy and endoscopic enteral stenting with self-expanding metal stents. Several studies have shown that surgical approach has the advantage of a more durable relief of symptoms and the need of fewer re-interventions, at the cost of higher procedure-related risks and longer hospital stay. On the other hand, enteral stenting provides rapid clinical improvement, but have the limit of higher stent dysfunction rate due to tumor ingrowth and a subsequent need of frequent re-interventions. Recently, a third way has come from interventional endoscopic ultrasound, through the development of endoscopic ultrasound-guided gastroenterostomy technique with lumen-apposing metal stent. This new technique may ideally encompass the minimal invasiveness of an endoscopic procedure and the long-lasting effect of the surgical gastrojejunostomy, and brought encouraging results so far, even if prospective comparative trial are still lacking. In this Review, we described technical aspects and clinical outcomes of the above-cited therapeutic approaches, and discussed the open questions about the current management of MGOO.
Statistical Methods for Estimating Normal Blood Chemistry Ranges and Variance in Rainbow Trout (Salmo gairdneri), Shasta Strain

Gaussian and nonparametric (percentile estimate and tolerance interval) statistical methods were used to estimate normal ranges for blood chemistry (bicarbonate, bilirubin, calcium, hematocrit, hemoglobin, magnesium, mean cell hemoglobin concentration, osmolality, inorganic phosphorus, and pH for juvenile rainbow (Salmo gairdneri, Shasta strain) trout held under defined environmental conditions. The percentile estimate and Gaussian methods gave similar normal ranges, whereas the tolerance interval method gave consistently wider ranges for all blood variables except hemoglobin. If the underlying frequency distribution is unknown, the percentile estimate procedure would be the method of choice.
Socio-economic aspects of the tiger shark diving industry within the Aliwal Shoal Marine Protected Area, South Africa

Understanding socio-economic aspects of the tiger shark Galeocerdo cuvier diving industry, including information on participant expectations, experiences and expenditure, is necessary for the effective management of the Aliwal Shoal Marine Protected Area on the east coast of South Africa. Between January and December 2007, a total of 2 133 tiger shark dives was conducted by 1 065 divers (95% CI = 946-1 198). Data were collected by means of the administration of a semi-structured survey questionnaire to 197 (18.6%) dive participants. Respondents indicated that the direct value of tiger shark diving to the Aliwal Shoal region was R12 405 274 (95% CI = R10 777 324-14 228 541). A total of 1 000 Monte Carlo simulations was used to estimate confidence intervals. On a ranking from one (poor) to five (excellent), the average participant response to overall quality of dive and standard of dive operator was 4.6 and 4.7 respectively. The majority of divers (98.0%) observed a tiger shark, at an average of four per dive. Although tiger sharks approached to an average distance of 1.6 m from divers, the majority (95.9%) felt safe and enjoyed the experience. The majority of interviewees (88.5%) supported the use of chumming for a closer ‘tiger shark experience’.
Molecular clocks, biogeography and species diversity in Herichthys with evaluation of the role of Punta del Morro as a vicariant brake along the Mexican Transition Zone in the context of local and global time frame of cichlid diversification

Using molecular dated phylogenies and biogeographic reconstructions, the species diversity, biogeography and time frame of evolution of the genus Herichthys were evaluated. In particular, we test the role of Punta del Morro (PdM) as a vicariant brake along the Mexican Transition Zone in the context of local and global time frame of cichlid diversification using several sets of calibrations. Species diversity in Herichthys is complex and the here employed dating methods suggest young age and rapid divergence for many species while species delimitation methods did not resolve these young species including both sympatric species pairs. Based on our molecular clock dating analyses, Herichthys has colonized its present distribution area significantly prior to the suggested vicariance by PdM (10–17.1 Ma vs. 5 to 7.5 Ma). The PdM constraint is in conflict with all other paleogeographic and fossil constraints including novel ones introduced in this study that are, however, congruent among each other. Our study demonstrates that any cichlid datings significantly older or younger than the bounds presented by our analyses and discussion have to be taken as highly questionable from the point of view of Middle American paleogeography and cichlid biogeography unless we allow the option that cichlid biogeography is completely independent from ecological and geological constraints.
Lack of association between the GNB3 rs5443, HIF1A rs11549465 polymorphisms, physiological and functional characteristics

The aim was to examine the association between the hypoxia‐inducible factor‐1α (HIF1A) gene and the guanine nucleotide binding protein beta polypeptide 3 (GNB3) gene polymorphisms and the endurance/power athlete status and relative aerobic capacity. Another goal of this study was to reveal the connection between GNB3, blood pressure (BP), body composition and body mass index (BMI). Two hundred thirty‐eight people participated in this study: 148 elite athletes (men = 107, women = 41) and 90 controls (men = 51, women = 39). The athletes were divided into two groups: the power and the endurance athletes. BMI and body fat percentage (fat%) were calculated. Fifty of the athletes underwent an incremental treadmill test to exhaustion; BP was monitored before and after the test. There were differences in the genotype frequencies of HIF1A between the endurance and the control group (ProPro: 64% vs.79%, ProSer: 27% vs.19%, SerSer: 9% vs. 2%; p = .0351); in the allele prevalences among the three groups (Pro: 87% vs. 77% vs. 88%; Ser: 13% vs. 23% vs. 12%; p = .0103) and between the endurance and control group (p = .0049) as well. The GNB3 allele proportions differed in the three groups (C: 74% vs. 61% vs. 71%, T: 26% vs. 39% vs. 29%; p = .0436). There were no connections between the genotypes and the relative aerobic capacity and neither between GNB3 genotypes and BP, BMI and fat%. The connection of GNB3 T allele to the endurance performance still remained contradictable.