Miracle-dz/UniFAR-FTUs huggingface dataset:
Longitudinal differences in iron deposition in periaqueductal gray matter and anterior cingulate cortex are associated with response to erenumab in migraine

Objectives The objective of this longitudinal study was to determine whether brain iron accumulation, measured using magnetic resonance imaging magnetic transverse relaxation rates (T2*), is associated with response to erenumab for the treatment of migraine. Methods Participants (n = 28) with migraine, diagnosed using international classification of headache disorders 3rd edition criteria, were eligible if they had six to 25 migraine days during a four-week headache diary run-in phase. Participants received two treatments with 140 mg erenumab, one immediately following the pre-treatment run-in phase and a second treatment four weeks later. T2* data were collected immediately following the pre-treatment phase, and at two weeks and eight weeks following the first erenumab treatment. Patients were classified as erenumab responders if their migraine-day frequency at five-to-eight weeks post-initial treatment was reduced by at least 50% compared to the pre-treatment run-in phase. A longitudinal Sandwich estimator approach was used to compare longitudinal group differences (responders vs non-responders) in T2* values, associated with iron accumulation. Group visit effects were calculated with a significance threshold of p = 0.005 and cluster forming threshold of 250 voxels. T2* values of 19 healthy controls were used for a reference. The average of each significant region was compared between groups and visits with Bonferroni corrections for multiple comparisons with significance defined as p < 0.05. Results Pre- and post-treatment longitudinal imaging data were available from 28 participants with migraine for a total of 79 quantitative T2* images. Average subject age was 42 ± 13 years (25 female, three male). Of the 28 subjects studied, 53.6% were erenumab responders. Comparing longitudinal T2* between erenumab responders vs non-responders yielded two comparisons which survived the significance threshold of p < 0.05 after correction for multiple comparisons: the difference at eight weeks between the erenumab-responders and non-responders in the periaqueductal gray (mean ± standard error; responders 43 ± 1 ms vs non-responders 32.5 ± 1 ms, p = 0.002) and the anterior cingulate cortex (mean ± standard error; responders 50 ± 1 ms vs non-responders 40 ± 1 ms, p = 0.01). Conclusions Erenumab response is associated with higher T2* in the periaqueductal gray and anterior cingulate cortex, regions that participate in pain processing and modulation. T2* differences between erenumab responders vs non-responders, a measure of brain iron accumulation, are seen at eight weeks post-treatment. Less iron accumulation in the periaqueductal gray and anterior cingulate cortex might play a role in the therapeutic mechanisms of migraine reduction associated with erenumab.
Computational design of multiaxial tests for anisotropic material characterization

Although anisotropic materials provide more capabilities for mission‐ and application‐tailored design and functional flexibility to final structures than regular isotropic materials, the directional behavior of the anisotropic materials further complicates their inelastic and damage behavior. Such a non‐linear behavior can be effectively observed and characterized by multiaxial testing, but how to design a multiaxial test for material characterization given a specimen remains an untouched issue. This paper presents a methodology that numerically designs the loading path of a multiaxial testing machine to characterize anisotropic materials. The multiaxial test must be able to exhibit quantities used to characterize materials as distinctly as possible. The proposed methodology formulates distinguishability and uniqueness as such quantities by first analyzing the specimen on a continuum basis with finite element method and then applying singular value decomposition. Associating the distinguishability and uniqueness with the informativeness of the loading path, the design problem is formulated such that an effective loading path can be found efficiently by a standard optimization method. Numerical examples first investigate the validity of the distinguishability and the uniqueness as performance measures to evaluate loading paths. The efficacy of the proposed methodology has been then confirmed by analyzing it with and applying it to design problems. Copyright © 2007 John Wiley & Sons, Ltd.
Single-lead VDD pacing: a literature review on short-term and long-term performance

ABSTRACT Introduction VDD pacing system was introduced more than 30 years ago. Its use is considered by the 2021 European Society of Cardiology guidelines on cardiac pacing as a potential alternative to dual chambers system for patients with atrioventricular block and normal sinus node function. Areas covered In this article, we performed a narrative review of current literature in order to identify the strengths and weaknesses of this pacing system. VDD system allows the maintenance of AV synchronous pacing and its hemodynamic advantages. Some disadvantages may be related to the non-negligible incidence of atrial undersensing and the possible subsequent need for upgrade to DDD system. On the other hand, shorter implantation time and lower complications rate may be advantages. Expert opinion In the modern pacing era, VDD pacing system struggles to find its own space. However, it may still be considered as a valuable alternative to a dual-chamber pacemaker for selected patients, in specific clinical scenarios.
Combined effects of high temperature and pesticide mixture exposure on free-swimming behaviors and hepatic cytochrome P450 1A expression in goldfish, Carassius auratus

ABSTRACT The synergy between multiple compounds and other stressors, including heat, creates volatility and greater unpredictability than standard single-chemical toxicity testing, especially in the case of pesticides and metabolites which might contain several noxious ingredients resulting in adverse ecological effects. To address this, the aim of this study was to examine the dose- and time-dependent effects of low- and high-dose pesticide mixture (metalachlor, linuron, isoproturon, tebucanazole, aclonifen, atrazine, pendimethalin, azinphos-methyl) and heat stress co-exposure (22°C control/32°C treatment for 4-week) on free-swimming behaviors and cumulative actionless time (CAT) of goldfish. Behavioral analysis showed a dose- and time-dependent decrease in distance swam, as well as a subsequent increase in CAT. Vertical and horizontal spatial behavioral use were affected under heat and pesticides co-exposure conditions. In 3- and 4-week(s) exposure groups, horizontal spatial behavioral use demonstrated elevated time spent in the lower third of the aquarium. Similarly, during 3- and 4-week(s) exposure (32°C control and 32°C high doses) vertical spatial behavioral use was found to increase time spent in the outermost edges of the aquarium. In all treatment groups, the final condition factor (KM) showed significant attenuation when compared to the initial KM. However, there was an unclear relationship between heat/pesticide co-exposure and growth most notably in 32°C high-dose groups. In addition, the expression of hepatic cytochrome P450 1A mRNA was significantly higher in pesticide-exposed groups. Taken together, data demonstrated that co-exposure with low- or high-dose pesticide mixture and heat stress significantly impacted natural swimming patterns, which over time might result in the broader population and ecological effects.
Comparable humoral and cellular immunity against Omicron variant BA.4/5 of once‐boosted BA.1/2 convalescents and twice‐boosted COVID‐19‐naïve individuals

The fourth vaccination dose confers additional protective immunity against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection in individuals with no prior coronavirus disease‐19 (COVID‐19). However, its immunological benefit against currently circulating BA.4/5 is unclear in individuals who have received a booster shot and been infected with Omicron variant BA.1/2. We analyzed immune responses in whom had been boosted once and did not have COVID‐19 (n = 16), boosted once and had COVID‐19 when BA.1/2 was dominant in Korea (Hybrid‐6M group, n = 27), and boosted twice and did not have COVID‐19 (Vx4 group, n = 15). Antibody binding activities against RBDo BA.1 and RBDo BA.4/5, antigen‐specific memory CD4+ and CD8+ T‐cell responses against BA.4/5, and B‐cell responses against SARS‐CoV‐2 wild‐type did not differ statistically between the Hybrid‐6M and Vx4 groups. The humoral and cellular immune responses of the Hybrid‐6M group against BA.4/5 were comparable to those of the Vx4 group. Individuals who had been boosted and had an Omicron infection in early 2022 may not have high priority for an additional vaccination.
Diagnosis of echinococcosis by detecting circulating cell-free DNA and miRNA

ABSTRACT Introduction Diagnosis of echinococcosis is difficult and usually performed based on clinical findings, imaging, and serological test. However, all of them have limitations, especially in follow-up approaches. Areas covered Detection of cell-free DNA (cfDNA) and micro-RNA (miRNA) is currently a hot topic for diagnosis of echinococcosis diseases. For detecting cell-free DNA in echinococcosis patient’s samples such as sera, some techniques are based on next-generation sequencing (NGS), DNA-deep sequencing, some are based on PCR-based methods, and a few works related to the detection of miRNA for the diagnosis of human echinococcosis. Expert opinion In the detection of cell-free DNA in echinococcosis patient’ samples, NGS and DNA-deep sequencing have shown high level of sensitivity, but are not suitable for routine clinical examination as they are expensive and inaccessible in the majority of endemic areas. However, PCR-based methods have shown a sensitivity of about 20–25%. To improve the sensitivity of these tests, improving the DNA extraction method, designing appropriate primers for detecting short-length fragments of circulating DNA, using a higher volume of a serum sample, and application of more sensitive PCR methods are recommended. In the field of miRNA detection, further works are recommended.
Need for affect, need for cognition, and the desire for independence

The last decade has witnessed a significant rise in European secessionist movements leading to considerable political turmoil (e.g., protest, repression, imprisonment of political leaders). While scholars have identified a number of economic and political factors that influence the support for secessionist movements, fewer studies have focused on its psychological roots. Using evidence from Catalonia, this paper investigates the role of two fundamental individual traits, Need for Affect (NFA) and Need for Cognition (NFC), in influencing the support for Catalan independence. It analyzes a large representative sample of adult Catalans in 2013, during the peak of the secessionist movement, to examine the influence of NFA and NFC, and their interaction, on the intention to vote in favor of seceding from Spain. Results indicate that individuals who have high levels in NFA and those who have high levels of NFA and low levels of NFC are more likely to support independence. In other words, individuals who have low levels of both NFA and NFC have the lowest support for independence. In conclusion, these findings highlight the importance of considering individual differences in psychological motives in order to fully understand support for secessionist movements.
On Finite Sample Distributions of the Empirical Likelihood Estimator and the GMM Estimator

The distributions of the Maximum Empirical Likelihood (MEL) estimator and the Generalized Method o Moments (GMM) estimator for the coe cient o one endogenous variable in a linear structural equation are evaluated numerically.Tables and gures are given for enough values of the parameters to cover most of interest.Comparisons of the distributions of the MEL estimator and the GMM estimator with their asymptotic expansions are made.
Center-to-limb variation of scattering polarization in molecular solar lines: Observations and modeling

We present center-to-limb measurements of the scattering polarization observed in molecular lines of C 2 and MgH in the spectral range between 515.7 and 516.1 nm, together with a radiative transfer model for the formation of these lines. The observations were performed in July 2000 with THEMIS inside the south polar limb. We were able to measure the polarization at distances between 1 arcsec and 50 arcsec from the solar limb. The lines appear as very weak absorption features in the intensity spectrum but their linear polarization clearly dominates in the polarization spectrum. We introduce here a simple radiative transfer model which allows to interpret the observed center-to-limb variations of both the intensity and linear polarization. The basic assumption is that molecular lines are formed higher in the photosphere than the continuous photospheric radiation. Molecules are thus illuminated by the polarized continuum photospheric radiation field. We account for a possible Hanle eect due to weak unresolved magnetic fields but we neglect depolarizing collisions. The model depends on four parameters which are determined by fitting both the intensity and polarization in 9 molecular lines of the observed spectral domain. Making use of the dierential Hanle eect in the dierent lines of C2 we show that the C2 lines are aected by the Hanle eect due to a weak unresolved magnetic field. Its mean strength is on the order of 15 Gauss in the upper photosphere and increases to values on the order of 50 Gauss at larger depths. These results are in good agreement with those derived previously (Faurobert et al. 2001) from the linear polarization of the SrI 460.7 nm line which was observed simultaneously. Such a weak field has almost no eect on the MgH lines.
High occurrence of Enterococcus faecalis, Enterococcus faecium, and Vagococcus lutrae harbouring oxazolidinone resistance genes in raw meat-based diets for companion animals – a public health issue, Switzerland, September 2018 to May 2020

Introduction Enterococci harbouring genes encoding resistance to florfenicol and the oxazolidinone antimicrobial linezolid have emerged among food-producing animals and meat thereof, but few studies have analysed their occurrence in raw meat-based diets (RMBDs) for pets. Aim We aimed to examine how far RMBDs may represent a source of bacteria with oxazolidinone resistance genes. Methods Fifty-nine samples of different types of RMBDs from 10 suppliers (three based in Germany, seven in Switzerland) were screened for florfenicol-resistant Gram-positive bacteria using a selective culture medium. Isolates were phenotypically and genotypically characterised. Results A total of 27 Enterococcus faecalis, Enterococcus faecium, and Vagococcus lutrae isolates were obtained from 24 of the 59 samples. The optrA, poxtA, and cfr genes were identified in 24/27, 6/27 and 5/27 isolates, respectively. Chloramphenicol and linezolid minimum inhibitory concentrations (MICs) ranged from 24.0 mg/L–256.0 mg/L, and 1.5 mg/L–8.0 mg/L, respectively. According to the Clinical and Laboratory Standards Institute (CLSI) breakpoints, 26 of 27 isolates were resistant to chloramphenicol (MICs ≥ 32 mg/L), and two were resistant to linezolid (MICs ≥ 8 mg/L). Multilocus sequence typing analysis of the 17 E. faecalis isolates identified 10 different sequence types (ST)s, with ST593 (n = 4 isolates) and ST207 (n = 2 isolates) occurring more than once, and two novel STs (n = 2 isolates). E. faecium isolates belonged to four different STs (168, 264, 822, and 1846). Conclusion The high occurrence in our sample of Gram-positive bacteria harbouring genes encoding resistance to the critical antimicrobial linezolid is of concern since such bacteria may spread from companion animals to humans upon close contact between pets and their owners.
Comparison of magnetic resonance imaging findings among sciatica patients classified as centralizers or non-centralizers

ABSTRACT Objective To compare if the degenerative findings from MRI differ between the sciatica patients classified as centralizers (CEN) and non-centralizers (Non-CEN) according to the McKenzie Method of mechanical diagnosis and therapy. Study Design A cross-sectional study. Methods Patients (N = 100) referred to a spine clinic of a single tertiary hospital for specialist consultation for sciatica. The McKenzie-based assessment was performed by the mechanical diagnosis and therapy-trained physiotherapists. Clinical data and prevalence of lumbar MRI findings were compared between the groups. Results There was no significant difference in leg pain intensity between the groups. The Non-CEN had significantly more intense back pain, mean 56 (SD 30) and were more disabled 44 (SD 15) compared to the CEN mean 41 (SD 25) and mean 31 (11), measured with a visual analogue scale (0–100), and the Oswestry Disability Index (0–100), respectively. The CEN had more severe degenerative findings on MRI than the Non-CEN: vertebral end-plate changes were 63% and 43%; mean Pfirrmann’s disc degeneration lumbar summary score was 12.8, and 10.6; and severity score of total damage was 12.0 and 10.1, respectively. There were differences neither in disc contour changes nor nerve root stenosis on MRI. Conclusions Sciatica patients classified as non-centralizers had significantly more severe back pain, and were significantly more disabled than centralizers, who instead had more severe degenerative findings on MRI. Thus, classification to non-centralizers by the McKenzie method seems not predict higher incidence of degenerative findings on MRI compared to centralizers.
Acceptability and retention of the key population‐led HIV treatment service for men who have sex with men and transgender women living with HIV in Thailand

In Thailand, where the HIV epidemic is concentrated among key populations (KPs), particularly men who have sex with men (MSM) and transgender women (TGW), an HIV service delivery model tailored to KPs was piloted. This study evaluated the acceptability and retention of clients who accepted and declined the KP‐led HIV treatment service.
Revisiting marine redox conditions during the Ediacaran Shuram carbon isotope excursion

The Neoproterozoic carbonate record contains multiple carbon isotope anomalies, which are the subject of intense debate. The largest of these anomalies, the Shuram excursion (SE), occurred in the mid‐Ediacaran (~574–567 Ma). Accurately reconstructing marine redox landscape is a clear path toward making sense of the mechanism that drives this δ13C anomaly. Here, we report new uranium isotopic data from the shallow‐marine carbonates of the Wonoka Formation, Flinders Ranges, South Australia, where the SE is well preserved. Our data indicate that the δ238U trend during the SE is highly reproducible across globally disparate sections from different depositional settings. Previously, it was proposed that the positive shift of δ238U values during the SE suggests an extensive, near‐modern level of marine oxygenation. However, recent publications suggest that the fractionation of uranium isotopes in ferruginous and anoxic conditions is comparable, opening up the possibility of non‐unique interpretations of the carbonate uranium isotopic record. Here, we build on this idea by investigating the SE in conjunction with additional geochemical proxies. Using a revised uranium isotope mass balance model and an inverse stochastic carbon cycle model, we reevaluate models for δ13C and δ238U trends during the SE. We suggest that global seawater δ238U values during the SE could be explained by an expansion of ferruginous conditions and do not require a near‐modern level of oxygenation during the mid‐Ediacaran.
Severity analysis of single-vehicle left and right run-off-road crashes using a random parameter ordered logit model

Abstract Objectives Single vehicle (SV) run-off-road crashes are a major cause of severe injury and fatality. Such crashes can result in different levels of severity depending on the direction (i.e., left or right) in which the vehicle runs off the road. This paper investigated the factors contributing to the crash severities of right run-off-road (R-ROR) and left run-off-road (L-ROR) SV crashes. Methods The study used SV crash data from the City of Charlotte, North Carolina, covering 2014 to 2017. Two separate random parameter ordered logit (RPOL) models were developed to estimate the contributing factors to R-ROR and L-ROR SV crash severities. The impact of the explanatory variables on the crash severity outcomes was quantified using the models’ direct pseudo-elasticities. Results The model results showed that male drivers, Driving Under Influence (DUI), motorcycles, and dry road surfaces were significant contributing factors to R-ROR and L-ROR SV crash severities. Specifically for the R-ROR model, speeding, reckless driving, 1–2 lanes, and older drivers increased crash severity. For the L-ROR model, phone distraction, crossed centerline/median, 3–4 lanes, rain, and dark unlighted roadway increased crash severity. Conclusions Based on the estimated parameters for the common significant variables in the two models, it was inferred that L-ROR SV crashes are more likely to result in severe crashes compared to R-ROR SV crashes. Hence, this study contributes to the literature on ROR SV crashes by providing additional insight into contextual factors influencing ROR crash severity for more effective countermeasures.
Touchscreen-Based Cognitive Training Alters Functional Connectivity Patterns in Aged But Not Young Male Rats

Abstract Age-related cognitive decline is related to cellular and systems-level disruptions across multiple brain regions. Because age-related cellular changes within different structures do not show the same patterns of dysfunction, interventions aimed at optimizing function of large-scale brain networks may show greater efficacy at improving cognitive outcomes in older adults than traditional pharmacotherapies. The current study aimed to leverage a preclinical rat model of aging to determine whether cognitive training in young and aged male rats with a computerized paired-associates learning (PAL) task resulted in changes in global resting-state functional connectivity. Moreover, seed-based functional connectivity was used to examine resting state connectivity of cortical areas involved in object-location associative memory and vulnerable in old age, namely the medial temporal lobe (MTL; hippocampal cortex and perirhinal cortex), retrosplenial cortex (RSC), and frontal cortical areas (prelimbic and infralimbic cortices). There was an age-related increase in global functional connectivity between baseline and post-training resting state scans in aged, cognitively trained rats. This change in connectivity following cognitive training was not observed in young animals, or rats that traversed a track for a reward between scan sessions. Relatedly, an increase in connectivity between perirhinal and prelimbic cortices, as well as reduced reciprocal connectivity within the RSC, was found in aged rats that underwent cognitive training, but not the other groups. Subnetwork activation was associated with task performance across age groups. Greater global functional connectivity and connectivity between task-relevant brain regions may elucidate compensatory mechanisms that can be engaged by cognitive training.