Miracle-dz/UniFAR-FTUs huggingface dataset:
Hepatitis C is associated with more adverse pregnancy outcomes than hepatitis B: A 7‐year national inpatient sample study

Prior international studies have shown mixed results regarding the association of hepatitis B and hepatitis C with adverse pregnancy outcomes. We performed an updated evaluation of the prevalence of associated adverse pregnancy outcomes and evaluated trends over time of diagnosis of chronic hepatitis B (HBV) and chronic hepatitis C (HCV) in pregnant women in a national database. All pregnant women with HBV and HCV were identified from the National Inpatient Sample database 2012 to 2018. Multivariate logistic regression analyses were performed to compare pregnancy‐related complications, including rates of preeclampsia/eclampsia, gestational diabetes, intrauterine growth restriction, antepartum/intrapartum hemorrhage, preterm labor, and Cesarean section. We evaluated all‐cause in‐hospital mortality, length of stay, and total cost of hospitalizations. A total of 28.7 million pregnancy‐related hospitalizations that met our eligibility criteria were identified, including 51,200 with HBV and 131,695 with HCV. In comparison with the uninfected controls, the HBV group was significantly more likely to develop gestational diabetes (12.94% vs. 6.94%, p < 0.001). The HCV group was more likely to have preterm labor (9.63% vs. 6.27%, p < 0.001), intrauterine growth restriction (6.04% vs. 2.89%, p < 0.001), longer length of stay (3.4 days vs. 2.7 days, p < 0.001), and higher hospitalization cost (15,052 dollars vs. 14,258 dollars, p < 0.001). These findings should inform counseling of women who are found to have HBV or HCV during pregnancy regarding the risk of adverse pregnancy outcomes and support the need for an interdisciplinary approach to optimize maternal and neonatal outcomes.
Development of a Monoclonal Antibody to Pig CD69 Reveals Early Activation of T Cells in Pig after PRRSV and ASFV Infection

The CD69 molecule, as an early activation marker of lymphocytes, is often used to assess the activation of cellular immunity. However, for pigs, an anti-pig CD69 antibody is not yet available for this purpose after infection or vaccination. In this study, a monoclonal antibody (mAb) against pig CD69 was produced by peptide immunization and hybridoma technique. One mAb (5F12) showed good reactivity with pig CD69 that was expressed in transfected-HEK-293T cells and on mitogen-activated porcine peripheral blood mononuclear cells (PBMCs) by indirect immunofluorescence assay and flow cytometry. This mAb did not cross-react with activated lymphocytes from mouse, bovine, and chicken. Epitope mapping showed that the epitope recognized by this mAb was located at amino acid residues 147–161 of pig CD69. By conjugating with fluorochrome, this mAb was used to detect the early activation of lymphocytes in PRRSV- and ASFV-infected pigs by flow cytometry. The results showed that PRRSV infection induced the dominant activation of CD4 T cells in mediastinal lymph nodes and CD8 T cells in the spleen at 14 days post-infection, in terms of CD69 expression. In an experiment on ASFV infection, we found that ASFV infection resulted in the early activation of NK cells, B cells, and distinct T cell subsets with variable magnitude in PBMCs, spleen, and submandibular lymph nodes. Our study revealed an early event of lymphocyte and T cell activation after PRRSV and ASFV infections and provides an important immunological tool for the in-depth analysis of cellular immune response in pigs after infection or vaccination.
Selecting Developmentally Competent Human Blastocysts: From Basic Morphological Assessment to Morphokinetics and Preimplantation Genetic Testing for Aneuploidy Iryna Scienti ﬁ c Journal of Biology

of PGT-A has also reduced the miscarriage rate and shortened the time to achieve a pregnancy Abstract The ability to select human embryo with the highest implantation potential remains one of the greatest challenges in the management of In Vitro Fertilization patients. Here, we review current methods for embryo selection including blastocyst static morphological grading, analysis of embryo morphokinetic parameters, and preimplantation genetic testing for aneuploidy, and their impact on the success of clinical outcomes. We also discuss emerging non-invasive cell-free embryonic DNA examination of spent embryo culture media. Other experimental methods, such as metabolomics, proteomics, cumulus cells or trophectoderm biopsy sample transcriptomics remain controversial and not yet suitable for routine clinical application. These are thus beyond the scope of this review. Traditionally, embryos with superior morphology have been selected for transfer, but these static parameters have been diﬃ cult to measure objectively and to use for prediction of blastocyst implantation potential. The introduction of time-lapse imaging system and morphokinetic markers to the laboratory practice allowed to study embryo cell dynamics during early preimplantation development, enhancing the evaluation of embryo quality. Nevertheless, morphokinetic annotations and predictive algorithms might vary between clinics because of diﬀ erent culture environments, stimulation protocols and embryo developmental hallmarks, which overall limit their prognostic value for assessing embryo viability. Moreover, the use of an objective but invasive technique of preimplantation genetic testing for embryo aneuploidy has been shown to improve the selection of embryos with the most potential to implant and produce a healthy pregnancy. However, not all euploid embryos implant, likely owing to embryonic, endometrial factors, or both. Thus, at present, the combination of blastocyst testing for chromosomal abnormalities, analysis of embryo morphokinetics and morphological blastocyst scoring , as integrated approaches , will help to select developmentally competent embryos to maximize chances of successful reproductive outcome. Furthermore, implementing artiﬁ cial intelligence to the ﬁ eld of Assisted Reproductive Technology may standardize the embryo selection process for a more reliable prediction of embryo quality and
Is there Correlation between Subclinical Hypothyroidism and Diabetic Nephropathy?

Background: Diabetic patients have higher prevalence of subclinical hypothyroidism which may be associated with diabetic complications. The aim of this study was to investigate the association of subclinical hypothyroidism with diabetic nephropathy (DN) in type 2 diabetic patients. Patients and Methods: A total of 150 type 2 diabetic patients were selected. Fasting blood sugar, HbA1C, lipid profile, creatinine, free T4, TSH and urinary albumin to creatinine ratio (UACR) were assessed for all patients. Patients with UACR of ≥30 mg/gr or glomerular filtration rate (GFR) <60 ml/min were defined as diabetic nephropathy (DN). Subclinical hypothyroidism (SCH) was defined as an elevated TSH >4 m IU/L and free thyroxine (FT4) level in normal range. Results: In our study 7.3% of all patients had subclinical hypothyroidism. No significant difference was found between patients with and without DN in TSH level. There was no significant difference in GFR and rate of albuminuria between subclinical hypothyroid and euthyroid patients. TSH was not correlated with albuminuria and GFR. Conclusion: Subclinical hypothyroidism is not associated with DN in type 2 diabetes.
CCIDE_A_270453 415..422

1Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, People’s Republic of China; 2Faculty of Dentistry, Mansoura University, Mansoura, Dakahlia, Egypt; 3Faculty of Dentistry, University of Otago, Dunedin, Otago, New Zealand; 4Division of Dentistry, University of Manchester, Manchester, UK Abstract: The paradigms of contemporary caries management have shifted to minimal intervention dentistry. Conservative restorative procedures are recommended to replace the complete removal of all carious tissues in the management of dental caries. This article reports two clinical cases of conservative restorations of proximal caries. Different conservative cavity designs and restorative techniques were performed in the two cases. Proximal caries in posterior teeth were prepared using the box-only preparation technique or the proximal tunnel preparation technique. The cavities were restored directly with resin composites. The advantages of using the box-only preparation with fissure sealant included the maximum conservation of tooth hard tissues and the prevention of unnecessary tissue removal in the occlusal surface. The tunnel preparation preserved the marginal ridge and protected the restored tooth from fracture. The filling of composites into the tunnel demonstrated a better marginal adaptation than other restorative materials. These two techniques allowed for the preservation of more healthy dental hard tissue compared with traditional techniques. Potential problems in the restoration included the high incidence of the inappropriate removal of dental hard tissue, damage of the vital pulp and the microleakage due to the polymerization shrinkage. The solutions to minimize the risk of these problems in the operative procedures were discussed.
Analysis of Reversible Switching Capacitive DAC Based Low Power SAR-ADC

This paper presents an analysis of Reversible Switching Capacitive Digital to Analog converter (RSC-DAC) based low power Successive Approximation Register Analog to Digital Converter (SAR-ADC). The proposed structure involves, the QVDC (Quantum Voltage Differential Comparator) constructed using Simple Transconductance Amplifier (STA) technique , the RSC-DAC switching energy reduced by 93% contrast to the standard Charge Redistribution Switching Capacitive DAC (CRSC-DAC) method, and the Successive Approximation Register(SAR) control logic is designed with D-FF based shift register. The QVDC comparator allows very small voltage comparison, and consumes low power and area effective. The linearity parameters such as Integral Nonlinearity, Differential Nonlinearity and parasitic effect of the capacitor of the RSC-DAC is analyzed and improved by the new approach is named as Adaptive Random Code Generation (ARCG) Technique. The above overall design is implemented by TANNER-EDA tool in 250nm CMOS technology, consumes 1.74mW power at 60MS/s. The INL and DNL of the proposed structure is +0.18/-0.12 LSB and +0.11/-0.05 LSB respectively.
Controlled Growth of the Non-centrosymmetric Zn(3-ptz)2 and Zn(Oh)(3-ptz) Metal-organic Frameworks

Non-centrosymmetric single-crystal metal-organic frameworks (MOF) are promising candidates for phase-matched nonlinear optical communication, but typical hydrothermal synthesis produces small crystals with relatively low transmittance and poor phase matching. We study the effect of the metal-to-ligand molar ratio and reaction pH on the hydro-thermal synthesis of the non-centrosymmetric Zn(3-ptz)2 and Zn(OH)(3-ptz) MOFs with in-situ ligand formation. In acidic environments, we find that decreasing the amount of ligand below the stoichiometric molar ratio 1:2 also produces highly transparent single-crystal octahedrons of Zn(3-ptz)2. In alkaline environments, we obtain long rod-like Zn(OH)(3-ptz) crystals whose length exceeds previous reports by up to four orders of magnitude. Potential applications of these results in the development of MOF-based nonlinear optical devices are discussed.
Understandability Metric for Web Services

--This work is concerned with developing a quality index for Web service understandability. A major reason for poor search results in Web service repositories is the lack of proper descriptions for existing services and as a result such services are not counted in search results. We present a mechanism to specify and measure Web service interface quality. The mechanism includes metrics for both the registration and operation phases. The evaluation results show significant enhancement in the discovery process as a result of using the proposed mechanism.
Bulletin UASMV Horticulture, 68(1)/2011

Transgenic plums of Prunus domestica L. transformed with the Plum pox virus coat protein gene (PPV-CP) were the subjects of three experiments undertaken in Romania. In the first experiment PPV-CP transgenic clones C2, C3, C4, C5, C6 and PT3 were evaluated for Sharka resistance under high natural infection pressure. Transgenic clone C5, subsequently named „HoneySweet‟, showed high resistance to PPV. None of the C5 trees became naturally infected by aphids for more than ten years. The resistance of C5 was based on post-transcriptional gene silencing (PTGS). In the second experiment we assessed the effect of two heterologous viruses (Prunus necrotic ringspot virus and Prune dwarf virus) on the efficacy and stability of PTGS mediated resistance to PPV displayed by the C5 plum. The engineered resistance to PPV in C5 transgenic plums was stable and was not suppressed by the presence of the assayed heterologous viruses. Some PPV-CP transgenic plum clones that are susceptible to PPV including C2, C3, C4 and PT3 display a constitutive transcription of PPV-CP sequence. In the third experiment we used these plants to assess the environmental safety issues related to potential hazards concerning the emergence of PPV variants. The serological and molecular variability of PPV detected in transgenic and in conventional plums revealed that the transgenic plums do not affect the diversity of indigenous PPV populations. We suggest that the safety and efficacy data developed in Romania and other European countries and in the United States of America over the last 15 years justifies an expansion of the field tests in Romania and in other countries that are experiencing the damages to plum production caused by PPV.
The Role of Types of Anesthesia on Maternal Blood Parameters among Women Underwent Cesarean Section

Background: As the rates of caesarean births have increased, the type of cesarean section has gained importance. Objective: To compare between maternal pre/postoperative hematological parameters (hemoglobin and hematocrit level), in addition to blood transfusion needed among pregnant women that underwent elective cesarean section under spinal and general anesthesia. Patients and Methods: This study was carried out on 110 women underwent cesarean section during the period from the 1 st of June 2013 to the 1 st of July 2015 at Department of Obstetrics and Gynecology in Al-Noo'man Teaching Hospital in Baghdad. The study participants were randomly divided into two equal groups according to the type of anesthesia performed (55 cases for general anesthesia group and 55 cases for spinal anesthesia group). The changes in the maternal blood parameters including hemoglobin and hematocrit values in addition to blood transfusion needed were reported and compared between the two groups in pre and post-operative cesarean sections. Results : The preoperative hemoglobin and hemaotocrit was insignificantly difference in the two groups. The mean hemoglobin and hematocrit concentrations were significantly reduced in women with general anesthesia compared to spinal anesthesia. Mean hemoglobin and hematocrit concentrations loss in spinal anesthesia group was significantly lower than in general anesthesia group. The requirement of blood transfusion after surgery was significantly higher among women undergone cesarean section under general anesthesia compared to those with spinal anesthesia. Conclusion : Spinal anesthesia is a better choice of anesthesia as it reduces the amount of blood loss (less reduction in hemoglobin and hematocrit concentrations) and requirement of the post-operative blood transfusion.
THE PREVALENCE OF LONELINESS AMONG UNIVERSITY STUDENTS FROM FIVE EUROPEAN COUNTRIES DURING THE COVID-19 PANDEMIC

At universities for students, the COVID-19 pandemic and the introduced anti-pan-demic measures turned out to be psycho-traumatic factors that increased the experience of loneliness. The purpose of the study was to investigate the prevalence of the phenomenon of loneliness among university students in five European countries, taking into account the variety of anti-COVID measures during the COVID-19 pandemic. Using the UCLA Loneliness Scale, questionnaires of 2316 students. In Russia, Poland and Ukraine a hard lockdown was introduced during the pandemic. Lithuania (in the first months) did not undertake severe restrictions, and for a long time the danger of the SARS-CoV-2 virus was not recognized in Belarus. The students in Lithuania and Belarus, 33 and 35 points. Students from Poland, Russia and Ukraine: 38, 37, 37 points, respectively. All respondents were
IMPACT OF SUPPLEMENTATION OF ZINC ON THE OUTCOME OF PREGNANCY IN FEMALES SUFFERING FROM GESTATIONAL DIABETES

: Objective: The aim of this research work is to interrogate the impacts of supplementation of the zinc on the outcome of pregnancy in the females from the gestation diabetes. Methodology : We divided the 60 females suffering from the gestational diabetes in their 32nd week of gestation to receive the twenty-five-milligram zinc sulfate orally every day as research or placebo as control. Then, the comparison of the both groups carried out about the needs of the insulin doses, macrosomia & pre-eclampsia. Results: The requirement of the insulin & alterations in dosage during investigation were very low in the group of patients in comparison with the group of healthy controls. Females in the group of zinc supplementation had very low percentage of the large neonates in comparison with the healthy controls. There were no important disparities between the members of both groups about the rate of occurrence of pre-eclampsia & cesarean sections. Conclusions: This research work showed that the supplementation of the zinc in pregnancy females suffering from the diabetes during pregnancy period could decrease the requirements of insulin and improve the control of the blood glucose among these patients & it may decrease the incidence of macrosomia.
Progressive To Acute Social And Psychosocial Introversion Features And Behaviors Toward Alzheimer's Process

Social relation and communicational behavior, personal losses and the capacity of coping with these were studied in 57 women and 49 men with Alzheimer's disease. As a comparison group, equal number normally aged women and men were studied, matched by age, labor profile, schooling, and other factors (matched pairs). All Alzheimer patients passed through a phase of de-motivation, and subsequent perceptive dysfunction. The social relationship and communication profiles were significantly less versatile and lower than in the matched controls. The capacity of coping with these losses was remarkably impaired in Alzheimer patients. Significant personal losses were similar in both groups. We believe that a progressive psychosocial introversion behavior, together with the coping deficit, is the situation where a de-motivation for life takes place, followed by a perception auto-blockade, that will trigger the gradual cessation of the natural reinforcing mechanisms by which the neuronal networks are maintained and then a progressive disintegration of the brain functions will develop.
ITS2 REGION-BASED MOLECULAR IDENTIFICATION OF FUNGAL PATHOGENS IN EQUINE CORNEAL ULCERATION

This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories. Conjunctival swabs from 12 horses with corneal ulcerations admitted to the Veterinary Teaching Hospital, College of Veterinary Medicine, Purdue University were collected for examinations. 14 conjunctival swabs were analyzed by the conventional culture method and by generic PCR using universal fungal primers to amplify the internal transcribed spacer 2 (ITS2) genetic region followed by DNA sequencing. The conventional culture method revealed that two samples exhibited fungal growth that identified as Aspergillus sp. and Penicillium sp. while three conjunctival samples contained bacterial growth which was identified as Staphylococcus intermedius , Staphylococcus epidermidis and Streptococcus zooepidemicus. Interestingly, PCR followed by DNA sequencing of the biological specimens on the swabs identified fungal pathogens in 9/14 samples and Ascomycete species in 3/14 samples. In 2/14 samples, no fungal pathogen was identified using PCR. Fungal pathogens detected included Aspergillus sp. in six eyes, Penicillium sp. in one eye, Fusarium sp. in one eye and Cladosporium sp. in one eye. Our findings demonstrate a limitation of the conventional culture method as a diagnostic tool as a fungal pathogen was detected in only two samples by this method as compared to nine samples using PCR and DNA sequencing. Therefore, PCR followed by DNA sequencing is able to identify a greater spectrum of agents including fastidious organisms or organisms present in lower numbers within mixed infections than can be identified by culture. In conclusion, PCR combined by DNA sequencing not only proved to be an effective and rapid method for the diagnosis of fungal keratitis, but was also a more sensitive diagnostic tool compared to the conventional culture method. Our findings demonstrate that PCR, in particular semi-nested PCR, is a promising tool for faster diagnoses of fungal keratitis in affected horses. addition, equipment and reagents necessary to perform the PCR are not readily available in all institutes, and the cost of the PCR is more expensive than the conventional culture method. However, as the present study demonstrates, PCR is a powerful tool that should be considered as a complimentary technique to use with culturing and can be used as an alternative diagnostic technique in cases where the causative pathogen cannot be identified by the routine culture method.
Genetic polymorphism of candidate genes in pig meat production

H-FABP, LEPR and MC5R genes were suggested as candidate genes for fat content in pig meat. The aim of this study was to detect genetic variation in the porcine H-FABP, LEPR and MC5R genes by PCR-RFLP method in a group of pigs. Genotyping of pigs was done by PCR-RFLP methods. We identified three genotypes in the set of pigs, HH (0.504), Hh (0.412) and hh (0.084) for H-FABP (HinfI). Allele H showed higher frequency than allele h (0.710 vs. 0.290). Three genotypes were identified for the H-FABP (HaeIII) gene (DD - 0.194, Dd - 0.494, dd - 0.312). The allele D (0.441) showed slightly lower frequency than allele d (0.559). All three genotypes were identified for LEPR (HpaII) in the group of pigs (AA – 0.137, AB - 0.314, BB – 0.549). Higher frequency of LEPR gene was confirmed for allele B (0.706), as compared with allele A (0.294). We identified two genotypes for MC5R (BsaHI) in the group of pigs (AA - 0.348 and AG - 0.652), genotype GG was not found. As conforms with genotype structure, we recognize a higher frequency of allele A (0.674) as compared with allele G (0.326).
Regularization Path Algorithms for Detecting Gene Interactions

In this study, we consider several regularization path algorithms with grouped variable selection for modeling gene-interactions. When tting with categorical factors, including the genotype measurements, we often dene a set of dummy variables that represent a single factor/interaction of factors. Yuan & Lin (2006) proposed the groupLars and the group-Lasso methods through which these groups of indicators can be selected simultaneously. Here we introduce another version of group-Lars. In addition, we propose a path-following algorithm for the group-Lasso method applied to generalized linear models. We then use all these path algorithms, which select the grouped variables in a smooth way, to identify gene-interactions aecting disease status in an example. We further compare their performances to that of L2 penalized logistic regression with forward stepwise variable selection discussed in Park & Hastie (2006b).