Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of HIV-1 Vif in viral replication : translational regulation of APOBEC3G and RNA chaperone activity

The HIV-1 viral infectivity factor (Vif) is a small basic protein essential for viral fitness and pathogenicity. Vif allows productive infection of non-permissive cells (including most natural HIV-1 targets) by counteracting cellular cytosine deaminases APOBEC3G (A3G) and A3F by different mechanisms and thus preventing its incorporation into viral particles. The Vif-induced degradation of A3G through the proteasome pathway has been extensively studied, but little is known about the translational repression of A3G mRNA by Vif. After cellular co-transfection of A3G mRNA constructs mutated in their untranslated regions (UTRs) in presence or absence of Vif, and in conditions where the proteasome-induced degradation of A3G was inhibited, we show that the 5’-UTR of A3G mRNA is crucial for the translational inhibition by Vif. The core binding factor, CBF-, required to stabilize the Vif/A3G complex is dispensable for this specific repression. According to our previous secondary structural model of the 5’-UTR, the two distal stem-loop structures are sufficient for a complete translational inhibition of A3G. We show that residue K26 of Vif is critical for A3G neutralization, both for its proteasome-induced degradation and translation inhibition of itsmRNA. Interestingly, we observe a strict correlation between the cellular reduction of A3G through translation inhibition and the quantity of A3G incorporated into viral particles. Both mechanisms account for about 50% decrease of A3G in cell. Thus, we showed for the first time that A3G mRNA translational inhibition by Vif is a 5’-UTR mRNA-dependent mechanism, and that any of these two mechanisms, degradation or translation, is sufficient to restore viral infectivity. Regulating the translation of A3G could thus be considered as a new target to restore a functional expression of A3G and viral restriction.
DESIGNING A DIGITAL TEACHING MODULE BASED ON MATHEMATICAL COMMUNICATION IN RELATION AND FUNCTION

The purpose of this study is to design a digital module based on mathematical communication skills. This a development research which is carried out to determine the use of poor learning media and students’ low comprehensive skills in understanding mathematical topics associated with relations and functions. One of the solutions used to overcome this problem is by designing a digital teaching module using media. The research and development method consisting of Analysis, Design, Development, implementation, and Evaluation (ADDIE), were used to carry out this study. The results showed that the digital module is highly valid with a total expert validation of 95.1% and in the very good category. Also, the students' response to the digital module is in the very good category, with a total response criterion of 89.8%. Therefore, the designed digital module has the ability to improve students' independence in learning because its use is not limited to classrooms.
Masking and Inpainting: A Two-Stage Speech Enhancement Approach for Low SNR and Non-Stationary Noise

Currently, low signal-to-noise ratio (SNR) and non-stationary noise cause severe performance degradation for most of speech enhancement models. For better speech enhancement at the above scenarios, this paper proposes a two-stage approach that consists of binary masking and spectrogram inpainting. In the binary masking stage, we first obtain binary mask by hardening soft mask and then use it to remove time-frequency points that are dominated by severe noise. In the spectrogram inpainting stage, we use a CNN with partial convolution to perform inpainting on the masked spectrogram from the previous stage. We compared our approach with two powerful baselines, including Wave-U-Net and CRN, on a low SNR dataset containing lots of non-stationary noises. The experimental results show that our approach outperformed the baselines and achieved the state-of-the-art performance.
PENGARUH RELIGIUSITAS, KELOMPOK REFERENSI DAN PENGETAHUAN TERHADAP BANK SYARIAH PADA NASABAH BANK SYARIAH DKI JAKARTA

A Moeslem must be obidient with the law contained in the Qur’an and Hadith, including within the law of muamalah or in the other words using financial service. Fatwa MUI No. 1 tahun 2004 was already explained that interest in conventional financial institution these day were considered haram because it has the same practice as riba nasi’ah and we can’t use conventional financial institution if we had a sharia financial institution near our home that we reach easily. Intention to use sharia banking were closely related to religiosity and knowledge about sharia banking, also reference group because sharia banking havent’do much about doing commercial in any form of media. The method used in this study is multiple linear regression analyst, t test, F test at level of significant 5% and performed with SPSS 21. The result of this study showed that Reliogisity, Reference Group, and Knowledge About Sharia Banking has positive effect on consumer intention to use sharia banking partially. Also Reliogisity, Reference Group, and Knowledge About Sharia Banking has positive effect on consumer intention to use sharia banking simultaneously.
Protein-hormone interactions patterning the gynoecium

The gynoecium is among the most intricately patterned organs of the plant, comprisingdifferent tissue sub-structures, all with the purpose of facilitating propagation to the next generation. It is therefore representative of the complexity involved in the initiation and establishment of organ patterning and presents a unique model to study the mechanisms coordinating development. As with all other organs, the interplay between genetic and hormonal factors specifies carpel development. However, although much is known about the genetic components involved in carpel development, our understanding of hormonal regulation and the cross-talk between these two pathways is limited. Thus, the aim of this thesis has been to address this issue by obtaining an integrated view of the genetic and hormonal regulatory mechanisms which act to coordinate gynoecium development. It has done so using broadly two approaches: first, by characterising the transcription factor interactions which pattern the carpel, and second, by elucidating the cross-talk between these interactions and the plant hormone auxin. Further, it has also studied the role of auxin in carpel morphogenesis. Observations from this research have uncovered a novel auxin co-receptor complex formed by the transcription factors IND and ETT. The co-receptor binds the IAA molecule directly and exhibits specificity for IAA over the synthetic analogues NAA and 2,4-D. This coreceptor functions to coordinate the development of the style and stigmatic tissues of the carpel, possibly via the regulation of PID kinase. Further, this work has also identified novel roles in protein-protein dimerisation for the domains involved in this interaction. Analyses also indicate that this novel auxin signalling pathway may also be conserved in the Brassicaceae through the ETT orthologues in this family. Finally, this project has analysed how ETT’s role as an auxin receptor could be translated into precise spatiotemporal regulation of its target genes to specify the boundaries necessary for gynoecium patterning. Together, the results from this work have posed new questions as to the signalling mechanisms through which auxin coordinates its varied and numerous functions in plants.
Efficient ZVT cell for interleaved DC–DC converters

Interleaved DC–DC converters are extensively employed in high-power applications to reduce input/output current ripple, elements current stress, and improve reliability. In order to eliminate the switching losses in interleaved converters, zero voltage transition (ZVT) technique can be used. This study presents a new compact ZVT cell appropriate for interleaved converters. This cell employs a low number of elements while imposing no extra voltage and current stresses on the converter main semiconductors. The proposed cell is applied to an interleaved boost converter to achieve full soft switching feature. The resulting ZVT interleaved boost converter is completely analysed and its design procedure is provided. Also, the converter is compared with the latest prominent counterparts presented in literature. Besides, its operation and theoretical analysis are validated by a 200 W and 100 kHz prototype. Finally, the proposed ZVT cell is applied to other types of interleaved converters and a family of ZVT interleaved converters is introduced.
Studies of the Electronic, Optical, and Thermodynamic Properties for Metal-Doped LiH Crystals by First Principle Calculations

Abstract Hydrogen as a clean and abundant energy source with high energy density is considered as a promising solution to future energy crisis, although storage of hydrogen is still challenging. Lithium hydride can be an alternative for hydrogen storage because of its small volume and high storage capacities, although this material is unsuitable as hydrogen reservoir because of its high dehydriding temperature. The density functional theory calculations based on the first principle are applied to study the physical properties of LiH without and with different metal M (M=Al, Fe, and Ru). The M-substituted systems exhibit lower dehydriding temperatures than the pure LiH, and Li1−xAlxH may be the most suitable candidate for hydrogen reservoir owing to the high hydrogen content and low dehydriding temperature. The stability and thermodynamic properties for hydrogen storage are discussed for these systems. The kinetics and the optical activity in the visible and infrared regions are enhanced by the metal dopants, characterized by the M impurity bands in the band gaps of the doped systems.
Distribution and dynamics of endoplasmic reticulum-plasma membrane junctions in pancreatic acinar and pancreatic cancer cells

Endoplasmic reticulum (ER)-plasma membrane (PM) junctions are found in many different cell types. They function as important calcium (Ca2+) signalling structures, as sites of store-operated calcium entry (SOCE) and play many other crucial roles. Their distribution in polarised migrating pancreatic cancer cells, and in the polarised pancreatic acinar cells, was the subject of this investigation. In migrating cells, the specific spatial pattern of Ca2+ signalling has been shown to be of paramount importance, but is produced by an unknown mechanism that I aimed to investigate. In acinar cells, Ca2+ influx (via the SOCE complex) occurs basolaterally, whilst Ca2+ signals are generated apically, physically separating the two processes. The presence of the Ca2+ influx channel normally present in SOCE complexes (Orai1) in the apical region therefore, is a conundrum this investigation set out to untangle. I found that ER-PM junctions and SOCE complexes localise to the leading edge of migrating cells and are ideally positioned for Ca2+-dependent regulation of actin dynamics and focal adhesion turnover – processes crucial to migration. The appearance of junctions at the leading edge was saltatory, suggesting that it develops as a result of the formation of new junctions, not from a relocation of junctions from a more interior position. I also saw long distance translocation and dissolution of junctions in the retracting tails of migrating cells, in a manner similar to the dynamics of focal adhesions. In pancreatic acinar cells, PM-localised Orai1 was found on basal and lateral membranes, whilst apical Orai1 was found to be absent from the PM and instead in a structure just beneath it, co-localising with ER-located Ca2+-release channels.
Productive performance, egg-related indices, blood profiles, and interferon-Ɣ gene expression of laying Japanese quails fed on Tenebrio molitor larva meal as a replacement for fish meal

Abstract The present experiment was conducted to investigate effects of Tenebrio molitor (TM) as a replacement for fish meal on egg productive and qualitative traits, serum biochemical parameters, haematology and interferon-ɣ gene expression in laying quails. A total of 250 laying Japanese quails (Coturnix japonica), at 74 days of age were randomly allotted to five dietary treatments in a completely randomised design during a five-week period. Experimental treatments consisted of a basal diet (CTL) or basal diet supplemented with 25% (7.5 g/kg; TM25), 50% (15 g/kg; TM50), 75% (22.5 g/kg; TM75) and 100% (30 g/kg; TM100) TM larvae meal in replace of fish meal. Total replacement of fish meal with TM larva meal significantly increased daily feed intake (DFI) of laying quails compared to CTL (p < .05) but MT25, MT50 and MT75 decreased DFI in comparison to CTL (p < .05). Feed conversion ratio of quails fed with insect larva meal was not different from those received CTL. Serum cholesterol concentration was lower in quails of M75 and M100 groups than those fed on the other dietary treatments (p < .05). Haematological traits and interferon-ɣ gene expression of quails were not affected by dietary supplemental TM larva meal. It was concluded that dietary inclusion of TM larva meal can be used as replacement for fish meal in diet of laying quails up to 30 g/kg. Dietary supplementation of TM larva meal did not have any adverse effect on health status of laying quails. Highlights Dietary inclusion of TM larva meal can be used as replacement for fish meal in diet of laying quails. Dietary supplementation of TM larva meal did not have any adverse effect on health status of laying quails. Serum cholesterol concentration decreased by dietary supplementation of TM larva meal in laying quails.
A Contrastive Analysis of Conditionals in English and Moroccan Arabic: Potential problems when learning English conditionals by Moroccan EFL learners

： Learning to handle hypothetical situations in a new language is always difficult (Catford, et al., 1974). This rule holds true for Moroccan Arabic (henceforth MA) speakers learning English because grammatical devices in the two languages differ in almost all equivalent situations. For instance, while English verb forms are used to indicate tense in conditional sentences, MA uses them to indicate aspect. Adopting the typology of conditional constructions suggested by Dancygier (1999) and Dancygier & Sweetser (2005), this study provides a contrastive analysis of conditionals in English and MA to predict the possible errors EFL/ESL learners are likely to make while learning English. The analysis shows that the main discrepancy between English conditionals and MA conditionals lies in the verb form used by the two systems. Accordingly, if EFL/ESL learners are influenced by verb form in their L1, they are likely to face some challenges while learning English conditionals. That is, they are likely to use the past tense in the protases of English predictive conditionals and generic conditionals since the perfective form of the verb is used in the protases of these two types in MA. Concerning the protases of English non-predictive conditionals, Moroccan EFL/ESL learners are likely to use either the past tense or the present tense since both the perfective and the imperfective forms of the verb are possible in the protases of MA non-predictive conditionals. However, due to the fact that the perfective form is the prototypical form in the protases of conditionals in MA, EFL/ESL learners are likely to use the past tense more EFL learners apodoses of English conditionals since the prevalent form in the apodoses of MA conditionals is the imperfective.
Resistncia do cafeeiro a nematides: I - Testes em prognies e hbridos, para Meloidogyne exigua

Procurou-se identificar fontes de resistencia ao nematoide Meloidogyne exigua em cafeeiros derivados de hibridos interespecificos e em progenies de cafe arabica oriundas da Etiopia, em experimentos estabelecidos em duas localidades. A infestacao foi feita por dois processos usados com frequencia nesse tipo de trabalho. As avaliacoes do grau de ataque foram feitas quatro e 12 meses apos a infestacao, em Campinas e Ribeirao Preto, respectivamente, adotando-se escala variando de zero, para ausencia de galhas, a cinco pontos para grande intensidade de ataque. Verificou-se que a leitura feita aos quatro meses e tao eficiente quanto aos 12 meses, indicando a possibilidade de abreviar consideravelmente a duracao dessas avaliacoes. Notou-se, no experimento de Ribeirao Preto, uma reducao de aproximadamente 8% no crescimento das mudas infestadas e verificou-se, tambem, uma reducao de 11,5% e 12% nos pesos verde e seco das plantas, devido ao ataque do nematoide. De 1.692 plantas examinadas nos dois experimentos, selecionaram-se 106 (6,3%), caracterizadas pela ausencia de galhas. As populacoes derivadas dos hibridos entre Coffea arabica e C. canephora revelaram-se mais promissoras, contribuindo com maior numero de plantas resistentes. As plantas do experimento instalado em Campinas foram inoculadas tambem com a raca II de Hemileia vastatrix para o estudo da resistencia conjunta aos dois patogenos. Essas selecoes, em numero de 38, representam valioso material para o programa de melhoramento do cafeeiro.
The Relationship between Human Resources Practices and Organizational Performance in Pharmaceutical Industry of Thailand

Effective human resource management practices strongly influence employee performance by increasing employee productivity. Similarly, in developing countries proved that human resource is one of the key factors which contributes towards better employee performance and successful organization. High-quality employees provide a higher return on investment compare to the lower quality employees. Employees with the high potential could fill up the most valuable strategic positions in the organization in future and help in differentiation to gain competitive advantages. Additionally, talented human resources in the organization with high capabilities, knowledge and skills become the underlying source of learning a competitive advantage in the international marketplace. This study collected 320 questionnaires from the employees of the pharmaceutical industry using simple random sampling and after data screening 312 data utilized for further analysis. Structural equation modelling utilized to investigate human resource management practices and their effect on organization performance in the pharmaceutical industry in Thailand. The findings revealed contrary result in Thailand pharmaceutical industry that ethical climate does not have significant impacts on organizational performance. Therefore, it is important to look back the facts why this happens to observe them again with different industries in Thailand with related constructs.
Expression of the genes for arylamine N-acetyltransferases in mice

Arylamine N-acetyltransferases (NATs) are polymorphic enzymes involved in the metabolism of arylamine and hydrazine xenobiotics. Murine NAT isoenzymes are encoded by three Nat genes. Only Nat2 was previously known to be polymorphic, a single nucleotide substitution causing the slow acetylator phenotype in the A/J strain. The present study (Chapter 3) describes novel polymorphisms in all three Nat genes of the wild-derived inbred strains Mus spretus and Mus musculus castaneus . Functional analysis of hepatic and heterologously expressed NAT variants from the two strains demonstrated that M. m. castaneus is a fast and M. spretus a slow acetylator. A previously isolated 14.3kb Nat -positive mouse genomic clone of 129/Ola strain origin (clone A) was sequenced (Chapter 4). A 8.6kb Hind III fragment, containing the entire Nat2 coding region, was subcloned from clone A and used to generate a targeting construct for the production of Nat2 knock-out mice. Probes and appropriate PCR methodologies were developed for screening of embryonic stem cells for targeted incorporation of the construct (Chapter 4). Computational analysis of clone A sequence revealed a number of important elements around the Nat2 gene, including four microsatellite markers which were found to be polymorphic among different mouse strains. Combined with preliminary physical mapping work (Chapter 4), these markers will assist accurate localisation of the Nat genes on mouse chromosome 8. A non-coding exon was mapped 6.4-6.1kb upstream of the intronless Nat2 coding region. A functional polyadenylation signal was also identified 0.45kb downstream of the mouse Nat2 coding region. The genomic structure of other genes for mammalian NAT was also analysed, by comparison of ESTs and genomic sequences deposited in electronic databases. Reverse transcription PCR confirmed that Nat2 is expressed in many tissues, while Nat1 and Nat3 are expressed in the liver and spleen, respectively. The Nat2 transcript was also detected in mouse embryonic stem cells, suggesting a possible involvement of murine NAT2 early in development (Chapter 5). The elements constituting the core promoter of Nat2 were characterised, and a preliminary search for other transcriptional regulatory sequences was carried out, using reporter gene assays and electrophoretic mobility shift assays (Chapter 6).
First Clinical Study Using HCV Protease Inhibitor Danoprevir to Treat Naive and Experienced COVID-19 Patients

As coronavirus disease 2019 (COVID-19) outbreak, caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), started in China in January, 2020, repurposing approved drugs is emerging as important therapeutic options. We reported here the first clinical study using hepatitis C virus (HCV) protease inhibitor, danoprevir, to treat COVID-19 patients. Danoprevir (Ganovo) is a potent HCV protease (NS3/4A) inhibitor (IC50 = 0.29 nM), which was approved and marketed in China since 2018 to treat chronic hepatitis C patients. Ritonavir is a CYP3A4 inhibitor to enhance plasma concentration of danoprevir while it also acts as a human immunodeficiency virus (HIV) protease inhibitor at high doses. The chymotrypsin-like protease of SARS-CoV-2 shares structure similarity with HCV and HIV proteases. In the current clinical study (NCT04291729) conducted at the Nineth Hospital of Nanchang, we evaluated therapeutic effects of danoprevir, boosted by ritonavir, on treatment naive and experienced COVID-19 patients. The data from this small-sample clinical study showed that danoprevir boosted by ritonavir is safe and well tolerated in all patients. After 4 to 12-day treatment of danoprevir boosted by ritonavir, all eleven patients enrolled, two naive and nine experienced, were discharged from the hospital as they met all four conditions as follows: (1) normal body temperature for at least 3 days; (2) significantly improved respiratory symptoms; (3) lung imaging shows obvious absorption and recovery of acute exudative lesion; and (4) two consecutive RT-PCR negative tests of SARS-CoV-2 nucleotide acid (respiratory track sampling with interval at least one day). Our findings suggest that repurposing danoprevir for COVID-19 is a promising therapeutic option.