Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterisation of miRNA Expression in Dental Pulp Cells during Epigenetically-Driven Reparative Processes

Within regenerative endodontics, exciting opportunities exist for the development of next-generation targeted biomaterials that harness epigenetic machinery, including microRNAs (miRNAs), histone acetylation, and DNA methylation, which are used to control pulpitis and to stimulate repair. Although histone deacetylase inhibitors (HDACi) and DNA methyltransferase inhibitors (DNMTi) induce mineralisation in dental pulp cell (DPC) populations, their interaction with miRNAs during DPC mineralisation is not known. Here, small RNA sequencing and bioinformatic analysis were used to establish a miRNA expression profile for mineralising DPCs in culture. Additionally, the effects of a HDACi, suberoylanilide hydroxamic acid (SAHA), and a DNMTi, 5-aza-2′-deoxycytidine (5-AZA-CdR), on miRNA expression, as well as DPC mineralisation and proliferation, were analysed. Both inhibitors increased mineralisation. However, they reduced cell growth. Epigenetically-enhanced mineralisation was accompanied by widespread changes in miRNA expression. Bioinformatic analysis identified many differentially expressed mature miRNAs that were suggested to have roles in mineralisation and stem cell differentiation, including regulation of the Wnt and MAPK pathways. Selected candidate miRNAs were demonstrated by qRT-PCR to be differentially regulated at various time points in mineralising DPC cultures treated with SAHA or 5-AZA-CdR. These data validated the RNA sequencing analysis and highlighted an increased and dynamic interaction between miRNA and epigenetic modifiers during the DPC reparative processes.
Consequences of Recent Crises on the FLW Consumer Behaviour: A National Wide Representative Research—The Case of Romania

Research on food loss and waste (FLW) is quite limited in emerging countries, such as Romania, as the phenomenon, its consequences, and implications are not yet properly understood by both policy makers and consumers. Therefore, the aim of this paper is to conduct representative research in Romania to identify the main clusters of consumers depending on their food waste behaviour. By means of cluster analysis, we highlight the main consumer typologies in Romania, regarding their food waste behaviour. The main findings reveal the presence of three distinct segments of consumer typologies based on their food waste behaviour, including low-income young wasters, conscious middle-age wasters, and well-educated mature non-wasters. This study highlights the need for targeted interventions that consider the unique characteristics and behaviours of each segment to effectively reduce FLW at the household level. Overall, this paper provides important insights for academia and for policymakers in the field of FLW management. The food loss and waste behaviour has significant economic, social, and environmental impacts, and reducing it requires a common effort from all stakeholders. Reducing food waste presents challenges, but also presents an opportunity to improve economic, social, and environmental outcomes.
Forward and Backward Walking: Multifactorial Characterization of Gait Parameters

Although extensive literature exists on forward and backward walking, a comprehensive assessment of gait parameters on a wide and homogenous population is missing. Thus, the purpose of this study is to analyse the differences between the two gait typologies on a relatively large sample. Twenty-four healthy young adults participated in this study. By means of a marker-based optoelectronic system and force platforms, differences between forward and backward walking were outlined in terms of kinematics and kinetics. Statistically, significant differences were observed in most of the spatial–temporal parameters, evidencing some adaptation mechanisms in backward walking. Differently from the ankle joint, the hip and knee range of motion was significantly reduced when switching from forward to backward walking. In terms of kinetics, hip and ankle moment patterns for forward and backward walking were approximately mirrored images of each other. Moreover, joint powers appeared drastically reduced during reversed gait. Specifically, valuable differences in terms of produced and absorbed joint powers between forward and backward walking were pointed out. The outcomes of this study could represent a useful reference data for future investigation evaluating the efficacy of backward walking as a rehabilitation tool for pathological subjects.
Physics of alumimum particle combustion at zero-gravity

Experimental set up for studying alunlinum particle combustion consists of a free-falling platform with the combustion chamber, ruby laser for particle ignition, apparatus for combustion parameters registrations and control system. Combustion of single aluminum particles of 185-35011~ in diameter was studied at pressures l-70atm in atmospheres Oz+Nz, CO2 and Oz+Ar under the condition of zero-gravity. The following parameters were obtained: times of particle combustion, values of aluminum oxide accumulated on burning particle surface, sizes of combustion zone, some characteristics of condensed products. It was found that amount of accumulated aluminum oxide depends on gas content and significantly increases when pressure increases. It was obtained that the aluminum oxide is porous for gases Oz+Nz and CO 2. Experiments showed that time of particle combustion does not depends on pressure and depends on initial alumunum particle diameter d as d1T5 in atmospheres Oz+Nz and CO* and as d2 in atmosphere Oz+Ar. Size distributions of high dispersed fraction of aluminum oxide in combustion products were obtained. The obtained data can be useful in modelling of aluminum particle combustion.
Sugammadex and blood loss during cervical spine fusion surgery

Background and Aims: Sugammadex (SUG) has been associated with changes in coagulation studies. Most reports have concluded a lack of clinical significance based on surgical blood loss with SUG use at the end of surgery. Previous reports have not measured its use intraoperatively during ongoing blood loss. Our hypothesis was that the use of SUG intraoperatively may increase bleeding. Material and Methods: This was a single site retrospective study. Inclusion criteria were patients undergoing a primary posterior cervical spine fusion, aged over 18 years, between July 2015 and June 2021. The primary outcomes compared were intraoperative estimated blood loss (EBL) and postoperative drain output (PDO) between patients receiving SUG, neostigmine (NEO) and no NMB reversal agent. The objective was to determine if there was a difference in primary endpoints between patients administered SUG, NEO or no paralytic reversal agent. Primary endpoints were compared using analysis of variance with a P value of 0.05 used to determine statistical significance. Groups were compared using the Chi-squared test, rank sum or student's t test. A logistic regression model was constructed to account for differences between the groups. Results: There was no difference in median EBL or PDO between groups. The use of SUG was not associated with an increase in odds for >500 milliliters (ml) of EBL. Increasing duration of surgery and chronic kidney disease were both associated with an increased risk for EBL >500 ml. Conclusion: Intraoperative use of SUG was not associated with increased bleeding. Any coagulation laboratory abnormalities previously noted did not appear to have an associated clinical significance.
ECG-Free Heartbeat Detection in Seismocardiography Signals via Template Matching

Cardiac monitoring can be performed by means of an accelerometer attached to a subject’s chest, which produces the Seismocardiography (SCG) signal. Detection of SCG heartbeats is commonly carried out by taking advantage of a simultaneous electrocardiogram (ECG). SCG-based long-term monitoring would certainly be less obtrusive and easier to implement without an ECG. Few studies have addressed this issue using a variety of complex approaches. This study proposes a novel approach to ECG-free heartbeat detection in SCG signals via template matching, based on normalized cross-correlation as heartbeats similarity measure. The algorithm was tested on the SCG signals acquired from 77 patients with valvular heart diseases, available from a public database. The performance of the proposed approach was assessed in terms of sensitivity and positive predictive value (PPV) of the heartbeat detection and accuracy of inter-beat intervals measurement. Sensitivity and PPV of 96% and 97%, respectively, were obtained by considering templates that included both systolic and diastolic complexes. Regression, correlation, and Bland–Altman analyses carried out on inter-beat intervals reported slope and intercept of 0.997 and 2.8 ms (R2 > 0.999), as well as non-significant bias and limits of agreement of ±7.8 ms. The results are comparable or superior to those achieved by far more complex algorithms, also based on artificial intelligence. The low computational burden of the proposed approach makes it suitable for direct implementation in wearable devices.
Ultrasound guided erector spinae plane block versus thoracic epidural analgesia in traumatic flail chest, a prospective randomized trial

Background and Aims: Pain contributes to flail chest morbidities. The aim of this study was to compare the analgesic effects of ultrasound-guided erector spinae plane block (ESPB) with thoracic epidural analgesia (TEA) in patients with traumatic flail chest. Material and Methods: Sixty patients aged 18 – 60 years, ASA I-II, with unilateral flail chest were allocated into TEA group with a loading dose of 6 ml bupivacaine 0.25% and 2 μg/ml fentanyl and ESPB group with a loading dose of 20 ml bupivacaine 0.25% and 2 μg/ml fentanyl. This was followed by continuous infusion of 6 ml/hour bupivacaine 0.125% and 2 μg/ml fentanyl in both groups for 4 days. Pain scores at rest and on coughing, rescue analgesic consumption, PaO2/FIO2 ratio, PaCO2, pulmonary functions and adverse events were recorded. Results: In both groups, Visual Analog Scale (VAS) scores at rest and on coughing were significantly decreased after block initiation as compared to pre-block value. At all-time points, VAS scores at rest and on coughing were insignificantly different between both groups. PaO2/FIO2 ratio, forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV1) were significantly increased and respiratory rate, PaCO2, were significantly decreased as compared to pre-block values of the same group without significant difference between both groups. The incidence of hypotension was significantly higher in TEA group than ESPB group. Conclusions: ESPB can achieve adequate analgesia in traumatic flail chest equivalent to that of TEA with significant improvement of arterial oxygenation and pulmonary functions and without serious adverse effects.
Contrast-Enhanced Ultrasonography (CEUS) in Imaging of the Reproductive System in Dogs: A Literature Review

Simple Summary Contrast-enhanced ultrasound (CEUS) has been widely applied for reproductive imaging in humans and animals. This structured literature review aims to analyze the usefulness of CEUS in canine reproduction. Articles from 1990 to 2022 about CEUS in canine testicles, prostate, uterus, placenta, and mammary glands were searched on PubMed and Scopus. Thirty-six total results were found. The analysis of these works enlightened the usefulness of CEUS in testicular abnormalities and neoplastic lesions, except for characterizing tumors. CEUS in dogs was studied in animal models for human prostatic cancer treatment, while in veterinary medicine it was used to study prostatic vascularization and to assess prostatic diseases, showing good specificity for adenocarcinomas. CEUS differentiated the follicular phases in ovaries. In CEH-pyometra syndrome, it differentiated endometrium and cysts, and highlighted angiogenesis. CEUS was shown to be safe in pregnant dogs and was able to assess normal and abnormal fetal–maternal blood flow and placental dysfunction. In normal mammary glands, CEUS showed vascularization only in diestrus, with differences between mammary glands. CEUS was not specific for neoplastic versus non-neoplastic masses and for benign tumors, except for complex carcinomas and neoplastic vascularization. Works on CEUS showed its usefulness in several pathologies as a non-invasive, reliable diagnostic tool. Abstract The use of contrast-enhanced ultrasound (CEUS) has been widely reported for reproductive imaging in humans and animals. This review aims to analyze the utility of CEUS in characterizing canine reproductive physiology and pathologies. In September 2022, a search for articles about CEUS in canine testicles, prostate, uterus, placenta, and mammary glands was conducted on PubMed and Scopus from 1990 to 2022, showing 36 total results. CEUS differentiated testicular abnormalities and neoplastic lesions, but it could not characterize tumors. In prostatic diseases, CEUS in dogs was widely studied in animal models for prostatic cancer treatment. In veterinary medicine, this diagnostic tool could distinguish prostatic adenocarcinomas. In ovaries, CEUS differentiated the follicular phases. In CEH-pyometra syndrome, it showed a different enhancement between endometrium and cysts, and highlighted angiogenesis. CEUS was shown to be safe in pregnant dogs and was able to assess normal and abnormal fetal–maternal blood flow and placental dysfunction. In normal mammary glands, CEUS showed vascularization only in diestrus, with differences between mammary glands. CEUS was not specific for neoplastic versus non-neoplastic masses and for benign tumors, except for complex carcinomas and neoplastic vascularization. Works on CEUS showed its usefulness in a wide spectrum of pathologies of this non-invasive, reliable diagnostic procedure.
Update on persistent acute kidney injury in critical illnesses

ABSTRACT Acute kidney injury (AKI) affects about half of patients admitted to the intensive care unit (ICU), and worsens their short- and long-term outcomes. Apparently self-limiting AKI episodes initiate a progression toward chronic kidney disease (CKD) through cellular and molecular mechanisms that are yet to be explained. In particular, persistent AKI, defined in 2016 by the Acute Dialysis Quality Initiative as an AKI which lasts more than 48 h from its onset, has been correlated with higher morbidity and mortality, and with a higher progression to acute kidney disease (AKD) and CKD than transient AKI (i.e. AKI with a reversal within 48 h). This classification has been also used in the setting of solid organ transplantation, demonstrating similar outcomes. Due to its incidence and poor prognosis and because prompt interventions seem to change its course, persistent AKI should be recognized early and followed-up also after its recovery. However, while AKI and CKD are well-described syndromes, persistent AKI and AKD are relatively new entities. The purpose of this review is to highlight the key phases of persistent AKI in ICU patients in terms of both clinical and mechanistic features in order to offer to clinicians and researchers an updated basis from which to start improving patients’ care and direct future research.
Reservoirs of Staphylococcus spp. and Streptococcus spp. Associated with Intramammary Infections of Dairy Cows

To design cost-effective prevention strategies against mastitis in dairy cow farms, knowledge about infection pathways of causative pathogens is necessary. Therefore, we investigated the reservoirs of bacterial strains causing intramammary infections in one dairy cow herd. Quarter foremilk samples (n = 8056) and milking- and housing-related samples (n = 251; from drinking troughs, bedding material, walking areas, cow brushes, fly traps, milking liners, and milker gloves), were collected and examined using culture-based methods. Species were identified with MALDI-TOF MS, and selected Staphylococcus and Streptococcus spp. typed with randomly amplified polymorphic DNA-PCR. Staphylococci were isolated from all and streptococci from most investigated locations. However, only for Staphylococcus aureus, matching strain types (n = 2) were isolated from milk and milking-related samples (milking liners and milker gloves). Staphylococcus epidermidis and Staphylococcus haemolyticus showed a large genetic diversity without any matches of strain types from milk and other samples. Streptococcus uberis was the only Streptococcus spp. isolated from milk and milking- or housing-related samples. However, no matching strains were found. This study underlines the importance of measures preventing the spread of Staphylococcus aureus between quarters during milking.
Continuous Vital Signs Monitoring with a Wireless Device on a General Ward: A Survey to Explore Nurses’ Experiences in a Post-Implementation Period

Background: Nurse engagement, perceived need and usefulness affect healthcare technology use, acceptance and improvements in quality, safety and accessibility of healthcare. Nurses’ opinions regarding continuous monitoring appear to be positive. However, facilitators and barriers were little studied. This study explored nurses’ post-implementation experiences of the facilitators and barriers to continuously monitoring patients’ vital signs using a wireless device on general hospital wards. Methods: This study employed a cross-sectional survey. Vocational and registered nurses from three general wards in a Dutch tertiary university hospital participated in a survey comprising open and closed questions. The data were analysed using thematic analysis and descriptive statistics. Results: Fifty-eight nurses (51.3%) completed the survey. Barriers and facilitators were identified under four key themes: (1) timely signalling and early action, (2) time savings and time consumption, (3) patient comfort and satisfaction and (4) preconditions. Conclusions: According to nurses, early detection and intervention for deteriorating patients facilitate the use and acceptance of continuously monitoring vital signs. Barriers primarily concern difficulties connecting patients correctly to the devices and system.
Aeroacoustic Scattering via the Equivalent Source Method

A computational method for predicting the scattering of incident engine noise by an airframe in a uniform flow field has been developed. The method is based on the equations of time harmonic, linearized acoustics and employs the equivalent source method for solving an exterior Helmholtz equation boundary value problem. Details of the governing equation development and numerical solution process are presented. The solution methodology has been implemented in a computer program, called the Fast Scattering Code, which accepts user defined operating conditions and aircraft component geometries and simulates the scattered acoustic pressure and/or velocity fields at user specified locations. The code features fast numerical calculations that can be performed on personal computers or workstations for moderate excitation frequencies. Several aeroacoustics scattering cases involving model nacelle, wing, and airframe components are presented to demonstrate the program capabilities.
Multi-Agent Reinforcement Learning-Based Decision Making for Twin-Vehicles Cooperative Driving in Stochastic Dynamic Highway Environments

In the past decade, reinforcement learning (RL) has achieved encouraging results in autonomous driving, especially in well-structured and regulated highway environments. However, few researches pay attention to RL-based multiple-vehicles cooperative driving, which is much more challenging because of dynamic real-time interactions and transient scenarios. This article proposes a Multi-Agent Reinforcement Learning (MARL) based twin-vehicles cooperative driving decision making method which achieves the generalization adaptation of the RL method in highly dynamic highway environments and enhances the flexibility and effectiveness of collaborative decision making system. The proposed fair cooperative MARL method pays equal attention to the individual intelligence and the cooperative performance, and employs a stable estimation method to reduce the propagation of overestimated joint $Q$-values between agents. Thus, the twin-vehicles system strikes a balance between maintaining formation and free overtaking in dynamic highway environments, to intelligently adapt to different scenarios, such as heavy traffic, loose traffic, even some emergency. Targeted experiments show that our method has strong cooperative performance, also further increases the possibility of creating a harmonious driving environment.
Zircon and Apatite U‐Pb Constraints on the Tectonic Affinity and Metamorphic History of the Blueschist‐Facies Ambelakia Unit, Mt. Ossa, Greece

In the Aegean region, the Cycladic Blueschist Unit (CBU) and the Ambelakia unit (Mt. Ossa, Thessaly, Greece) represent early Cenozoic subduction‐related HP‐LT metamorphic complexes exhumed in the back‐arc of the Hellenic subduction zone. The Ambelakia unit has been linked to the classic CBU in the Cyclades; however, the tectonic affinity, structural position, nature of the protolith, or timing of metamorphism for these rocks in eastern Thessaly remains largely unresolved. This study provides detailed new insights into both the provenance, protolith age, and tectonic affinity of the Pelagonian, Ambelakia, and Olympos‐Ossa tectonic units by integrating U‐Pb detrital zircon (DZ) and detrital apatite (DA) data in a structural context. DZ results suggest the existence of distinct metasedimentary units, spanning in depositional age from Carboniferous to Late Cretaceous, with provenance signatures that support a correlation with the classic CBU and strengthen the argument for a similar pre‐subduction tectonic relationship. Depth‐profiling analysis reveals metamorphic rims that record HP‐LT metamorphism in the Ossa Ambelakia during the Paleocene‐Eocene. Apatite U‐Pb data from the metasedimentary units preserve a detrital signature similar to the DZ signatures, indicating that apatites were not reset (<450 C) nor recrystallized during subduction metamorphism. These new data suggest that the Ambelakia unit is a lateral equivalent of the CBU subduction complex; however, this unit experienced distinctly lower pressure and temperature conditions during metamorphism than the along‐strike classic CBU.
Immunopathology of Extracellular Vesicles in Macrophage and Glioma Cross-Talk

Glioblastomas (GBM) are a devastating disease with extremely poor clinical outcomes. Resident (microglia) and infiltrating macrophages are a substantial component of the tumor environment. In GBM and other cancers, tumor-derived extracellular vesicles (EVs) suppress macrophage inflammatory responses, impairing their ability to identify and phagocytose cancerous tissues. Furthermore, these macrophages then begin to produce EVs that support tumor growth and migration. This cross-talk between macrophages/microglia and gliomas is a significant contributor to GBM pathophysiology. Here, we review the mechanisms through which GBM-derived EVs impair macrophage function, how subsequent macrophage-derived EVs support tumor growth, and the current therapeutic approaches to target GBM/macrophage EV crosstalk.