Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of genomic alterations with cisplatin resistance (cisR) in advanced germ cell tumors (aGCT)

4510 Background: 20% of patients (pts) with aGCT progress after initial chemotherapy (chemo), displaying at least partial cisR. We sought to characterize the genetic alterations in aGCT and identify markers of cisR. Methods: aGCT pts with available tumor tissue (except pure teratoma or malignant transformation) were eligible. Cisplatin sensitive (cisS) pts had a CR to chemo +/- surgery (if pathology = necrosis or teratoma) or PR + negative tumor markers lasting one year or more. CisR pts had non-teratoma GCT at post-chemo surgery or at relapse. Cis status was inevaluable (IE) if followup (f/u) was too short (without progression) or standard initial chemo was not given. Whole exome sequencing (WES) in 19 pts (10 cisR, 9 cisS) identified several mutations and copy number changes, which we confirmed by targeted exon-capture sequencing of 341 cancer-related genes (MSK-IMPACT). MSK-IMPACT was then preformed for 69 additional pts. Results on all 88 pts were analyzed for associations with clinicopathologic featu.
Effect of pre-treatments on the germination of jasmin box (Phillyrea latifolia) seeds in Greece

The major problem in seedlings production of jasmin box (Phillyrea latifolia) is the poor and irregular germination due to seed dormancy. The objective of the present study was to evaluate methods of seed pre-treatments to increase germination. The effect of different solutions of KOH and NaOH, wood ash lye, GA3 and cold or warm stratification on germination were evaluated. Sets of four replicates of 25 seeds were soaked for 24 hours in KOH and NaOH solutions of 0.2, 0.5, 1 and 2 %, in wood ash lye solutions of 33, 66, 133, 166, 532 g L-1 and in GA3 solutions of 250, 500, 1000, 2,000, 3,000 mg L-1. A control set of seeds was imbibed in water for 48 hours. All sets went through zero, two, four and six months of cold (2-4 °C) or warm (15 °C) stratification. The seeds were incubated in petri dishes filled with moistened sand, at two alternating temperatures (20/15 and 30/20 °C) with a light period of 16 hours. From the results we conclude that: Chemical pre-treatments with KOH and ash lye and warm stratification increased germination to 96 %. Treatment with water and warm stratification also increased germination to 79 %. Treatment with 250 mg L-1 GA3 and six months warm stratification increased germination to 70 % while higher concentrations had a negative effect. Cold stratification did not break dormancy. Optimum germination temperature in a growth chamber was 15-20 °C, while temperature at 20-30 °C inhibited germination.
Bacteriological and antibiogram of AmpC producing Enterobacteriaceae isolated from abattoir.

Owing to the continued threat of antimicrobial resistance, it is critical that farmers and clinicians take the necessary steps to use antibiotics rationally in their respective practices, and only opt for it when all other options must have been exhausted. AmpC enzymes mediate bacteria resistance to the cephamycins such as cefotetan and cefoxitin which are important antibiotics used clinically to treat and manage bacterial related infections. This study phenotypically evaluated the prevalence and antibiogram of Enterobacteriaceae that produced AmpC enzymes. Fifty (50) anal swab samples from the anal region of cows in an abattoir in Abakaliki, Nigeria were bacteriologically analyzed. The isolation and identification of bacteria isolates were carried out using standard microbiology techniques. Antibiogram was evaluated using disk diffusion technique while AmpC enzyme production was detected using the ceftazidime imipenem antagonism test. Our results show that a total of 7 (14%) E. coli and 12 (24%) isolates of Klebsiella species was isolated. The antibiogram results showed that the isolated Klebsiella species and E. coli isolates exhibited reduced susceptibility to cloxacillin (100%), ertapenem (83.3%), cefoxitin (66.7%) and ceftazidime (58.3%) for Klebsiella species; and cefoxitin (71.4%), ertapenem (28.6%) and ceftazidime (28.6%) for isolates of E. coli. AmpC enzyme production was detected in 5 (71%) isolates of E. coli and 9 (75%) isolates of Klebsiella species. The AmpC producing E. coli and Klebsiella species were multiply resistant to over 4 antibiotics found in the class: fluoroquinolones, carbapenems, aminoglycosides and penicillins. Antibiotic usage in animal husbandry allows drug resistant bacteria such as those that produce AmpC enzymes to evolve and spread. This has implication for the general public since AmpC producing bacteria are notably resistant to 2nd generation cephalosporins which are used clinically for the treatment of serious bacterial infections. Continuous monitoring of the antibiotic resistance profile in abattoir and poultry isolates is recommended as a panacea to contain the problem of antibiotic resistance in the non-hospital environment.
Identification of The Dimensions of Bank 4.0 Experiential Quality Based on Millennial Customer Perceptions

The development of the Bank 4.0 industry that is reflected through increased experiential quality, which is started with the identification of the dimensions of Bank 4.0 experiential quality is getting popularity for millenial. This research attempt to discover the dimensions of Bank 4.0 experiential quality based on perceptions of millennial customers. Bu surveying 673 millenials and adjusted to the characters of Bank 4.0 and millennial generation this study aims to theoretical and empirically testing the hyotehesis by using SMPLS. Results revelaed that functional quality, convenience, innovations, trust, value, risk mitigation, and security have been empirically proven as dimensions of Bank 4.0 experiential quality based on millennial customer perception.This research was only conducted in the city of Malang, so that research with larger demographic and geographic data sets is needed in order to describe the perceptions of millennial customers more accurately. Further research can be developed with new dimensions that may emerge along with the development of Bank 4.0 in both commercial banks and fintech companies.
Identification of drought stress tolerance in wild species germplasm of rice based on leaf and root morphology

Abstract Drought is the major abiotic constraint to the rice production in the rain-fed areas across Asia and sub-Saharan Africa. Wild species of Oryza offer a wide spectrum of adaptive traits and can serve as potential donors of biotic and abiotic stress tolerance. At the Punjab Agricultural University, we are maintaining an active collection of 1630 accessions of wild species germplasm (AA, CC, BBCC and CCDD) of rice. These accessions were screened to assess genetic variation for drought tolerance under field conditions. Severe water stress was imposed at the late vegetative stage by withholding water initially for 25 d and then extended further to 35 d during kharif season in the years 2013–14 and 2015–16. The tolerance score for drought stress was based on the extent of leaf rolling and leaf drying. Based on the 2 years’ data, seven accessions from Oryza rufipogon, four from Oryza longistaminata and one each from Oryza officinalis and Oryza latifolia were found tolerant to drought stress. These selected accessions were further phenotype for root morphology. The average root length among the selected accessions ranges between 36 and 80 cm and the number of primary roots vary from 30 to 87 cm. The O. rufipogon accession IRGC 106433, O. longistaminata accession IRGC 92656A, O. officinalis accession IRGC 101152 and O. latifolia accession IRGC 80769 showed approximately 2–2.5 times longer root length and number than the indica rice elite cultivar PR121. The results indicated potentiality of selected wild species germplasm for conferring drought tolerance to the elite cultivars.
The Impact of COVID‐19 on Consumer Food Waste

Abstract Perhaps no phenomenon has so quickly and radically altered household production parameters and daily food patterns as the onset of the COVID-19 pandemic We contemplate the immediate and longer-term implications of this public health crisis on the amount of food wasted by consumers We conclude that the pandemic and its aftermath may improve household skills and management practices in a manner that reduces day-to-day household food waste However, pandemic-driven disruptions may induce larger intermittent purges of food due to changes in work patterns and foodservice and food retailing availability We recommend several steps to reduce waste as the pandemic unfolds
Morphological and Biochemical Properties Useful for Identification of Erwinia chrysanthemi

Rhizome rot or tip over disease of banana incited by Erwinia chrysanthemi is a serious disease causing rotting of newly planted rhizomes with failure to sprout, stunting and yellowing of young plants. It is a major constraint for commercial cultivation of banana. Several workers have reported in the fast from India. Edward et al., (1973) noticed „Tip over‟ or „bacterial rot‟ in a small piece of land with alluvial soil located close to Yamuna River for the first time on variety Basarai banana in 1968 from India, and isolated a strain of Erwinia carotovora from rotting parts of rhizome. Chattopadhyay and Mukherjee (1986) reported the pseudostem of banana caused by Erwinia chrysanthemi pv. paradisiaca on Gaint Governor in West Bengal. Lakshmanan and Mohan (1986) observed tip over disease or head rot caused by Erwinia carotovora in Tamil Nadu in North Arcot and Trichy districts. Dickey and Victoria (1980) reported that, the strain from M. paradisiaca in Colambia was very similar to E. chrysanthemi on the basis of biochemical and physiological properties. Snehalatharani and Khan, (2010) reported the bacterium showing positive for pectate degradation on CVP medium, growth at 36 0 C and 39 0 C, catalose production and negative for indole production. Further they gave positive result for the production of acid from various agars such as lactose, trehalose, maltose and cellobiose and soft rot of inoculated potato slices. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 10 (2017) pp. 343-348 Journal homepage: http://www.ijcmas. com
The concept of prime number and the strategies used in explaining prime numbers

The teaching of mathematics does not only require the teacher to have knowledge about the subject, but the teacher also needs mathematical knowledge that is useful for the teaching and explaining thereof, as the teacher’s knowledge effects the students’ knowledge. A teacher should use appropriate mathematical explanation to be understood well by her/his students. In the study reported on here we investigated how prospective mathematics teachers defined the concept of prime number and which strategies they employed to explain the concept. The study was a descriptive survey within qualitative research. Forty-eight participants took part in the study and all completed the abstract algebra courses where they learned about the concept in question. The data collection tool was a form comprising 3 open-ended questions challenging what the concept of prime number was and how this concept could be explained to secondary/high school students. The data were analysed and the results show that the preservice teachers experienced great difficulty in defining the concept of prime number and that they used rules to explain prime numbers. Keywords: explanatory strategies; prime number; prospective mathematics teachers
ACADEMICS ‘STAYING ON’ POST RETIREMENT AGE IN ENGLISH UNIVERSITY DEPARTMENTS OF EDUCATION: OPPORTUNITIES, THREATS AND EMPLOYMENT POLICIES

ABSTRACT In the UK, a default retirement age no longer exists and more people choose to ‘stay on’ in their academic posts. ‘Staying on’ poses opportunities and threats in the academic labour market. Older academics can make a positive contribution to their institution through their expertise and experience. By continuing to work, paying tax and keeping healthy, they may directly and indirectly reduce social health and welfare costs. Alternatively, in a context where academic jobs may be decreasing, older workers may be positioned as limiting the employment and promotion opportunities for younger colleagues by staying on. Drawing on twelve in-depth semi-structured interviews with academics who have stayed working in university education departments, this paper explores these issues alongside policy-related questions about employment in the sector.
Female Entrepreneurs in the Hospitality Industry: A Panel Causality Analysis of EU Countries

This study investigates the determinants of female entrepreneurship (FE) in the tourism and hospitality industry in 30 European countries. Based on annual observations for the period 2006–2016 and using panel causality techniques, the study explores the impact of tourism receipts, GDP per capita, the Global Entrepreneurial Index (GEI), policies related to institutional and cultural issues, childcare and gender parity related to skills and entrepreneurship on FE. The results of robust second-generation panel methods indicate the existence of long-run cointegration among FE, GDP, tourism receipts and other variables of interest. The long-run elasticities of tourism receipts, GDP, GEI, childcare and the female–male ratio of access to human capital formation in terms of training obtained from the panel fully modified ordinary least squares (FMOLS), panel dynamic ordinary least squares (DOLS) and the pooled mean group estimator (PMG) estimation are significant in the context of FE. The study concludes that pivotal policy interventions are needed to ensure that family policies and procedures, social strategies and tax structures do not discriminate female entrepreneurs. JEL: C32, C33, J16, O52, Z30
MYRICETIN ISOLATED FROM TURBINARIA ORNATA AMELIORATES ROTENONE INDUCED PARKINSONISM IN DROSOPHILA MELANOGASTER

Objective : Parkinson’s disease (PD) is a neurodegenerative disorder which affects the elderly population. Free radicals overproduction, oxidative stress, apoptosis, inflammation and abnormalities in mitochondria are critical mediators of the neuronal degeneration. In the present study neuroprotective activity of myricetin, a flavonoid isolated from brown seaweed Turbinaria ornata have been investigated in rotenone induced experimental PD models of Drosophila melanogaster. Methods : Male fruit flies ( Drosophila melanogaster ) were fed with an effective dose of 0.1% myricetin three hours before to the treatment with 500 µM of Rotenone (LD 50) for seven days and on 8 th day through behavioral analysis the neuroprotective effect of myricetin was investigated for motor coordination in fruit flies. Lipid peroxidation was analyzed by estimating the levels of TBARS. Oxidative stress was determined by estimating the activities of enzymatic antioxidants superoxide dismutase, catalase, and glutathione peroxidase along with the level of reduced glutathione. Dopamine level was estimated in HPLC column detected at 280 nm with UV detectors and degree of apoptosis was studied apoptotic marker Bcl-2, Bax, caspases-3 and 9, cytochrome c and β-actin expressions in the whole body homogenate of fruit flies of experimental groups homogenized in 500μL of 0.1 M phosphate buffers (ice cold, pH, 7.4) containing 1 mmol EDTA. Results : Myricetin maintains the positive behavioral patterns against motor impairments due to the rotenone toxicity, it creates a balance in oxidant and antioxidant status, reduces the oxidative stress and inhibits apoptosis to retard neurodegeneration and maintains the dopamine level with a significant (p<0.05) difference compared to the rotenone treated group. Conclusion : The flavonoid myricetin by reducing the oxidative stress, maintaining the enzymatic antioxidants status and by inhibiting apoptosis prevents the degeneration of dopaminergic neurons. The dopaminergic neurons prevention reduces the depletion of dopamine and thereby promotes the muscular coordination and psychological well being of fruit flies of experimental group. Further in depth molecular level studies are in need to explore the preventive mechanisms of myricetin in Parkinson’s disease.
Abyssal Pathways and the Double Silica Maximum in the Northeast Pacific Basin

This study examines causes of the double silica maximum in the deep interior Northeast Pacific Basin using a stochastic Lagrangian tracer model based on steady‐state advective fields and diapycnal diffusion established by a hydrographic inverse method that conserves potential vorticity and salinity. Lateral diffusion, unresolved by the inverse model, is adjusted for overall agreement with radiocarbon distribution. The double silica maximum in vertical profiles arises from an eastern‐intensified single maximum in the North Pacific Deep Water along the northern domain boundary (originating in the western Pacific), and a strong subarctic bottom source supplying silica to Upper Circumpolar Deep Water density surfaces that successively intersect the seafloor over a broad area east of 150°W, associated geostrophically with southward flow. The existence of the double silica maximum requires weak diapycnal transport in the deep interior, with broader implications for the conceptual picture of meridional overturning circulation in the North Pacific.
NK Cell Subsets in Health and Disease: New Developments

Natural Killer (NK) cells were discovered ca 1975, as the first group of lymphoid cells that were neither T cells nor B cells. Since then, the dissection of the biology of NK cells has been growing exponentially with many seminal discoveries from the identification of MHC class I-specific inhibitory receptors to the discovery of receptor-ligand pairs involved in NK cell activation and to the manipulation of NK cells in cancer. In this research topic, we asked a group of thought leaders in NK cell biology to review recent advances in their origins and biology, and their roles in cancer, infection and inflammation. Together, these 25 articles provide a timely survey of NK cells as critical immunologic components of health and disease. They will hopefully prompt further dialogue and developments in basic and translational immunology.
Designing environmental DNA surveys in complex aquatic systems: Backpack sampling for rare amphibians in Sierra Nevada meadows

1. Surveys for environmental DNA (eDNA) can provide an efficient and effective means of detecting aquatic organisms in various types of aquatic systems. 2. In the summer of 2017, the efficacy of a new, integrated eDNA backpack sampler to detect two native amphibians (Rana sierrae and R. cascadae) at risk was tested in complex mountain meadows in California. Samples were collected at 65 locations in 15 meadows where the target species were known to be present or were historically present. 3. Collection and preservation of individual samples took less than 10 min on average. Environmental DNA analysis methods detected each species at all meadows with visual detections (N = 11) except one with one frog seen away from sampling sites. Bayesian multi‐scale occupancy modelling indicated that conditional detection probabilities at the sample level ranged from 0.30 (CL 0.07–0.65) at meadow heads where no frogs were observed during visual surveys to 0.93 (CL 0.77–1.00) at the meadow foot with at least one frog observed in the vicinity. 4. Compared with visual surveys, eDNA methods more frequently detected amphibians at the sampling‐location scale. The improvement in detection using eDNA methods was most pronounced for samples collected at the downstream ends of meadows where water converges, where eDNA methods detected target species at 10 of 11 occupied meadows. 5. These results suggest that the addition of eDNA sampling to visual surveys in mountain meadows will improve survey accuracy and increase the probability of detecting rare frogs.
How low is too low? Postwash total motile sperm count effect on pregnancy outcomes in intrauterine insemination

Abstract Intrauterine insemination (IUI) is a frequently used method to treat couples with infertility. There is evidence of decreased pregnancy rates with a total motile sperm count (TMSC) of less than 10 million, yet there remains to be a consensus on semen parameters for which to recommend IUI in the infertile population. The aim of this study was to determine a minimum threshold of TMSC on semen analysis to offer IUI cycles. This is a retrospective cohort study of all IUI cycles at a private practice infertility centre over four years. Our primary outcome of interest was the presence of clinical pregnancy after each cycle. A total of 999 women underwent 2,169 IUI cycles. The overall clinical pregnancy rate was 19.8% per cycle. During the first IUI each woman underwent, there was an increase in clinical pregnancy with increasing TMSC (OR 0.44) for TMSC ≤1 M to (OR 0.99) for TMSC 6-10 M, compared to TMSC >10 M. Among all IUI with a TMSC between 6 and 10 M, pregnancy outcomes improved with morphology >4% (OR 0.84), compared to morphology <4% (OR 0.25), relative to TMSC >10 M. Using receiver operating characteristic curves, we did not identify a TMSC threshold to offer IUI, although there was a positive correlation between TMSC and IUI success.
Promising Practices for Improving Identification of English Learners for Gifted and Talented Programs

The underrepresentation of English learners (ELs) in gifted and talented programs is a societal and research problem that merits investigation. Three state departments of education and their state directors of gifted programs supported our access to 16 schools across nine districts. In these three states with gifted identification and programming mandates, ELs were proportionally represented in gifted and talented programs in the 16 schools we visited. Interview data from 225 participants revealed four themes: adopting universal screening procedures, creating alternative pathways to identification, establishing a web of communication, and using professional learning as a lever for change.
Photosynthesis and nutrient-use efficiency in response to N and P addition in three dominant grassland species on the semiarid Loess Plateau

Understanding the ecophysiological and nutrient-use strategies of dominant species is important for clarifying plant growth and an ecological process in their community under unbalanced N and P inputs. This study investigated effects of N and P addition [main plot: 0, 25, 50, and 100 kg(N) ha-1 year-1; subplot: 0, 20, 40, and 80 kg(P) ha-1 year-1] on leaf N and P contents (LNCa and LPCa), photosynthetic capacity (PNmax) and photosynthetic N- and P-use efficiencies (PNUE and PPUE) in three species (Stipa bungeana, Bothriochloa ischaemum, and Lespedeza davurica) in a semiarid grassland in China. At the assessing time, N addition alone significantly increased LNCa and LNCa/LPCa ratio of Stipa bungeana (C3 grass) only, while PNmax increased significantly in all three species. Under N addition, P addition caused significantly lower LNCa, but higher PNUE and PNmax in Bothriochloa ischaemum (C4 grass) and Stipa bungeana. The LNCa, PNmax, and PNUE of Lespedeza davurica (C3 legume) increased significantly after P addition regardless of N application. The LNCa/LPCa for optimum PNUE changed with species. The PNmax and PNUE of Bothriochloa ischaemum and Stipa bungeana had a peak when LNCa/LPCa attained ~ 11 and ~ 20. Lespedeza davurica tended to retain greater PNmax and PNUE at lower LNCa/LPCa. Our results indicated that the C3 grass was more sensitive to N and P addition than the C4 grass and the C3 legume in terms of leaf N and P contents and PNmax. Such interspecific variations of nutrient use in response to N and/or P addition are favorable for maintaining the diversity and the stability of the grassland community.