Miracle-dz/UniFAR-FTUs huggingface dataset:
Scavenger Receptor Class B Is Required for Hepatitis C Virus Uptake and Cross-Presentation by Human Dendritic Cells (cid:1)

Class B scavenger receptors (SR-Bs) bind lipoproteins and play an important role in lipid metabolism. Most recently, SR-B type I (SR-BI) and its splicing variant SR-BII have been found to mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells. In this study, we demonstrate that SR-BI is a key host factor required for hepatitis C virus (HCV) uptake and cross-presentation by human dendritic cells (DCs). Whereas monocytes and T and B cells were characterized by very low or undetectable SR-BI expression levels, human DCs demonstrated a high level of cell surface expression of SR-BI similar to that of primary human hepatocytes. Antibodies targeting the extracellular loop of SR-BI efﬁciently inhibited HCV-like particle bind- ing, uptake, and cross-presentation by human DCs. Moreover, human high-density lipoprotein speciﬁcally modulated HCV-like particle binding to DCs, indicating an interplay of HCV with the lipid transfer function of SR-BI in DCs. Finally, we demonstrate that anti-SR-BI antibodies inhibit the uptake of cell culture-derived HCV (HCVcc) in DCs. In conclusion, these ﬁndings identify a novel function of SR-BI for viral antigen uptake and recognition and may have an important impact on the design of HCV vaccines and immunotherapeutic approaches aiming at the induction of efﬁcient antiviral immune responses. instructions. To obtain monocyte-derived DCs, CD14 (cid:1) cells were cultured in the pres- ence of interleukin-4 (1,000 U/ml) and granulocyte-macrophage colony-stimu-lating factor (800 U/ml) (CellGenix, Freiburg, Germany). Immature DCs were collected on day 5 and assessed by cytoﬂuorimetric cell surface phenotyping using anti-human CD80-, CD83-, CD86-, and HLA-DR–phycoerythrin (PE)- conjugated antibodies. To study whether IgG puriﬁed from anti-SR-BI or control serum induces DC maturation, immature DCs were exposed to anti-SR-BI IgG or control IgG (50 (cid:3) g/ml) for 16 h. DC maturation was analyzed by ﬂow cytometry of CD80, CD83, CD86, and HLA-DR surface expression. SR expression. For the analysis of SR expression, cells (1 (cid:4) 10 5 cells/100 (cid:3) l) were incubated with antibodies directed against SR-BI, LOX-1, CD36, antigal- actosidase antibody (control IgG), or preimmune serum (control serum). Subsequently, cells were incubated with PE-conjugated anti-mouse IgG and analyzed by ﬂuorescence-activated cell sorter (FACS) as recently described (5). For the FACS analysis of SR-BI and SR-BII, cells were permeabilized with 0.05 to 0.1% saponin prior to incubation with rabbit polyclonal anti-SR-BI/II serum and allophycocyanin-conjugated anti-rabbit IgG. To demonstrate the speciﬁcity of polyclonal anti-SR-BI serum, CHO cells were transfected with pcDNA3 (control vector) or pcDNA-SR-BI by using liposome-mediated gene transfer (Lipo-fectamine; Invitrogen,
PII: S1010-7940(01)00801-6

Objective: Transmyocardial laser revascularization (TMLR) is used to treat patients with refractory angina considered unsuitable for conventional forms of revascularization. Using patient speci®c data from a single centre UK randomised-controlled trial, we aimed to determine whether, from a UK National Health Service (NHS) perspective, TMLR plus standard medical management is cost-effective when compared with standard medical management alone. Methods: One hundred and eighty-eight patients assessed as having refractory angina, and not suitable for conventional forms of revascularization were randomized to receive TMLR and medical management (94) or medical management alone (94). Costs to the UK NHS of TMLR (where appropriate), and all secondary sector health care contacts and cardiacrelated medication in the 12 months following randomization, were collected. Patient utility as measured using the EuroQol EQ-5D questionnaire was combined with 12-month survival data to generate quality adjusted life years (QALYs). Results: The mean cost per patient over the year from hospitalization for TMLR was £11,470 and for medical management alone was £2586, giving a cost difference of £8901 (95% con®dence interval (CI) £7502±£10,008: P , 0:0001). The mean QALY difference, in favour of TMLR was 0.039 (95% CI 20.033 to 0.113: P  0:268). This gives an incremental cost per QALY of over £228,000. Analysis of stochastic uncertainty and of sensitivity to gross changes in key parameters consistently produces very high costs per QALY. Conclusions: The policy implications are clear: for such patients TMLR is an inef®cient use of UK health service resources. This conclusion would not be changed by considerable improvements in effectiveness or reductions in cost. q 2001 Elsevier Science B.V. All rights reserved.
Receptor Promoter by Glucocorticoid a Receptor Transcriptional Regulation of the Mouse IL-7

Expression of the IL-7R (cid:1) -chain (IL-7R (cid:1) ) is strictly regulated during the development and maturation of lymphocytes. Glucocorticoids (GC) have pleiotypic effects on the growth and function of lymphocytes. Although GC have been reported to induce the transcription of IL-7R (cid:1) gene in human T cells, its molecular mechanism is largely unknown. In this study, we show that GC up-regulate the levels of IL-7R (cid:1) mRNA and protein in mouse T cells. This effect does not require protein synthesis de novo, because protein synthesis inhibitors do not block the process. Mouse IL-7R (cid:1) promoter has striking homology with human and rat, containing consensus motifs of Ikaros, PU.1, and Runx1 transcription factors. In addition, a conserved noncoding sequence (CNS) of (cid:1) 270 bp was found 3.6-kb upstream of the promoter, which was designated as CNS-1. A GC receptor (GR) motif is present in the CNS-1 region. Importantly, we show by reporter assay that the IL-7R (cid:1) promoter has speciﬁc transcription activity in T cells. This activity highly depends on the PU.1 motif. Furthermore, GC treatment augments the transcriptional activity through the GR motif in the CNS-1 region. We also demonstrate that GR binds to the GR motif by EMSA. In addition, by chromatin immunoprecipitation assay, we show that GR is rapidly recruited to endogenous CNS-1 chromatin after GC stimulation. These results demonstrate that GR binds to the GR motif in the CNS-1 region after GC stimulation and then activates the transcription of the IL-7R (cid:1) promoter. Thus, this study identiﬁes the IL-7R (cid:1) CNS-1 region as a IL-7R by GC, we dissected the mouse IL-7R (cid:2) promoter and characterized the molecular mechanism of transcription of the IL-7R (cid:2) gene. In this study, we demonstrated that GC positively induce the expression of IL-7R (cid:2) in mouse T cells and that ligand-activated GR binds to a GR motif in a conserved noncoding sequence (CNS) upstream of the IL-7R (cid:2) promoter, which was designated as CNS-1, and then activates the transcription of the IL-7R (cid:2) promoter. Thus, this study explores the molecular mechanism of IL-7R (cid:2) induction by GC and demonstrates that the CNS-1 region is a GC-responsive element of the IL-7R (cid:2) locus. results, taken together, indicate that GR protein binds to the GR motif in the IL-7R (cid:2) CNS-1 region in vitro.
using High-resolution correlations of strata within a sand-rich clinothem using grain fabric data, offshore New Jersey, USA

Trajectories of successive clinoform rollovers are widely applied to predict patterns of spatio-temporal sand distribution. However, the detailed internal architecture of individual clinothems is rarely documented. Understanding the textural complexities of complete topset-foreset-bottomset clinothem sequences is a key factor in understanding how and when sediment is transferred basinward. This study used high-resolution, core-based analyses of 267 samples from three research boreholes from quasi-coeval topset, foreset, and bottomset deposits of a single Miocene intrashelf clinothem recovered during Integrated Ocean Drilling Program (IODP) Expedition 313, offshore New Jersey, USA. Topset deposits were subdivided into three sedimentary packages based on grain character and facies analysis, consisting of upper and lower river-dominated topset process-regime packages separated by a middle wave- and storm-dominated process-regime package. Temporal variability in topset process regime exerts a quantifiable effect on grain character across the complete depositional profile, which was used here to correlate topset deposits with time-equivalent sedimentary packages in foreset and bottomset positions. River-dominated sedimentary packages have higher sand-to-mud ratios; however, the grain character of river-dominated sedimentary packages is texturally less mature than that of wave- and storm-dominated deposits. Differences in grain character between packages dominated by different process regimes increase basinward. The novel use of quantitative grain-character data allows intraclinothem time lines to be established at a higher resolution than is possible using chronostratigraphic techniques. Additionally, stratigraphic changes in grain character were used to refine the placement of the basal sequence boundary. These results challenge the idea that clinoform trajectories and stacking patterns are sufficient to describe spatio-temporal sand-body evolution across successive clinothems.
In Vitro Evaluation of Effect of Different Essential Oils in Management of Post-Harvest Fruit Rot of Banana (Musa Paradisiaca) Caused by Colletotrichum spp

A study was conducted to evaluate the in vitro efficacy of different essential oils in the management of post-harvest fruit rot of banana. It was carried out in completely randomized design (CRD) with three replications and six treatments at Nepal Plant Diseases and Agro Associates (NPDA). The each of six treatments: cinnamon oil (Cinnamomum verum), mustard oil (Brassica oleracea), castor oil (Ricinus communis), neem oil (Azadirachta indica), coconut oil (Cocos nucifera) and control were used at concentrations 200ppm, 500ppm and 1000ppm respectively. The radial growth of mycelium (mm) and percent growth inhibition (%) of Colletotrichum spp. was recorded. The decrease in the radial growth and increase in the percent growth inhibition was found in all the treatments except control as their concentration was increased, in which the lowest radial growth and the highest percent growth inhibition was found at 1000 ppm concentration. At 1000 ppm concentration, cinnamon oil shows the lowest radial growth and the highest percent growth inhibition (1.67mm and 98.15%) followed by mustard oil (54.00mm and 40.00%), neem oil (55.17mm and 38.70%), castor oil (55.83mm and 37.96%), coconut oil (61.17mm and 32.04%) and control (90mm and 0.00%) respectively.Thus, cinnamon oil is considered as a good essential oil in regards to a management of post-harvest disease of banana. Int. J. Appl. Sci. Biotechnol. Vol 9(3): 187-192.
I nvestigating the status of successful aging based on selection, optimization and compensation model and its relationship with some demographic variables in elderly population of Shiraz, southwest of Iran, 2018

and mean score of optimization and compensation (P value = 0.008). The number of children was also significantly correlated with change-based selection and overall successful aging (P value = 0.007 and P value = 0.043; respectively). Conclusion : Based on the results, the studied elderly people accounted for more than half of the score of successful aging. Successful aging scores were significantly correlated with variables such as age, level of education, marital status, and number of children. Hence, it is recom-mended to pay more attention to these variables in future research and planning.
PRBM_A_317389 1091..1099

Department of Education, Shandong Women’s University, Jinan, Shandong Province, People’s Republic of China Background: Previous studies have suggested that family communication quality is an important factor that can influence adolescents’ life satisfaction. However, relatively few studies have examined this relationship in the Chinese cultural context or explored the potential mechanisms underlying this relationship. Therefore, the purpose of this study was to investigate whether family communication quality is closely linked to Chinese adolescents’ life satisfaction and whether this link was mediated by autonomy and future orientation. Methods: This study recruited 442 Chinese college students (67.19% girls) as participants. Based on a longitudinal design, the participants reported family communication quality at Time 1 and reported autonomy, future orientation, and life satisfaction at Time 2. Results: The results of correlation analysis showed the existence of a positive correlation between family communication quality, autonomy, future orientation, and life satisfaction. The mediation models showed family communication quality predicted adolescents’ life satisfaction through the mediating effects of autonomy and future orientation. Furthermore, autonomy and future orientation not only independently mediated the relationship between family communication quality and life satisfaction but sequentially mediated this relationship. Conclusion: Autonomy and future orientation mediated the relationship between family communication quality and life satisfaction. The findings of the present study highlight the significance of elevating family communication quality and promoting adolescents’ development of autonomy and future orientation to enhance their life satisfaction.
Title: SOCIAL TOURISM AND SELF-EFFICACY Subtitle: Exploring links between tourism participation, job-seeking and unemployment

: Social tourism is assumed to provide important psychological benefits for economically and socially disadvantaged populations. This study examines empirically whether these individual benefits are associated with socioeconomic benefits to society by focusing on unemployed individuals. Psychological benefits are addressed in terms of self-efficacy, and socioeconomic benefits, in terms of job-search behaviour. Findings from mixed-methods data reveal that holidays create enabling environments, which bring about positive changes in participants’ self-efficacy, contributing to positive effects on their job-search behaviour. Positive effects are also identified with regard to behaviours towards alternative paths to employment, such as volunteering. Given that these behavioural changes comprise major determinants of reemployment, it is suggested that social tourism may hold potential for incorporation into existing unemployment policies.
ProductionDevelopment from Type 2 to Type 1 Cytokine T Cell /low - Peripheral Immature CD2

Immature myeloid and NK cells exist, and undergo cytokine-induced differentiation, in the periphery. In this study, we show that also immature CD2 (cid:1) /low T cells exist in peripheral blood. These cells produce the type 2 cytokines IL-13, IL-4, and IL-5, but not IFN- (cid:2) or IL-10, and, upon culture with IL-12- and TCR-mediated stimuli, differentiate to IL-13 (cid:3) IFN- (cid:2) (cid:3) cells producing high IL-2 levels, and ﬁnally IL-13 (cid:1) IFN- (cid:2) (cid:3) cells. The monokine combination IL-12, IL-18, and IFN- (cid:4) substitutes for TCR-mediated stimulation to induce the same differentiation process in both immature CD2 (cid:1) /low and primary mature CD2 (cid:3) IL-13 (cid:3) (cid:5) cells. IFN- (cid:4) is needed to maintain high level IL-2 production, which is conﬁned to type 2 cytokine-producing cells and lost in the IFN- (cid:2) (cid:3) ones. Upon TCR-mediated stimulation, IFN- (cid:2) (cid:3) cells are then induced to produce IL-10 as they undergo apoptosis. These data indicate that peripheral type 2 cytokine (cid:3) T cells are immature cells that can differentiate to effector IFN- (cid:2) (cid:3) cells following a linear monokine-regulated pathway identical with that previously described for NK cells. They deﬁne the cellular bases to support that cell-mediated immune responses are regulated not only via Ag-induced activation of mature effector cells, but also via bystander monokine-induced maturation of immature T cells. The Journal of Immunology, 2002, 169: 3061–3068.
DIVISION OF THE HUMANITIES AND SOCIAL SCIENCES

This paper examines the problem of identifying cost-effective solutions to problems characterized by multiple objectives. The traditional approach has been to minimize costs subject to feasibility constraints and a set of targets. This is compared with a multiobjective programming approach which treats the objectives as choice variables and cost as a parameter. To illustrate the two approaches, the problem of achieving environmental objectives is analyzed. The comparison reveals that the traditional cost-minimizing approach can generate solutions which are inefficient, in the sense that greater emission reductions could have been attained at the same cost. Because the solution sets to the two problems may differ, conditions are derived under which the two approaches yield a similar set of results.
antioxidants Anti-Sarcopenic Obesity Effects of Lonicera caerulea Extract in High-Fat Diet-Fed Mice

: Sarcopenic obesity is a combination of sarcopenia and obesity. Although several herbal extracts showed improvement on sarcopenia and obesity, respectively, there are few studies on sarcopenic obesity. Lonicera caerulea (honeysuckle berry, HB) can ameliorate metabolic disorders including obesity. However, its effects on sarcopenic obesity have not been reported yet. Thus, the aim of this study was to investigate whether HB extract might have any beneﬁcial effects on sarcopenic obesity in high-fat diet-induced mice. Forty-eight mice were classiﬁed into six groups and treated for eight weeks: (1) NC, normal diet control; (2) HC, high-fat diet control; (3) PC, high-fat diet with orlistat; (4) HB100, high-fat diet with HB extract at 100 mg/kg; (5) HB200, high-fat diet with HB extract at 200 mg/kg; and (6) HB400, high-fat diet with HB extract at 400 mg/kg. Body weight, fat accumulation, muscle mass, muscle strength, and mRNA expression of muscle atrophy were monitored. Compared with the HC group, HB administration showed anti-obesity properties. It reduced body weight gain and modulated serum biochemical parameters and tissue antioxidant enzymes. HB also increased muscle strength and muscle mass of hind legs. In addition, it decreased mRNA expression levels of Atrogin1 and MuRF1 as markers of muscle atrophy but increased PGC1 α and SIRT1 as markers of muscle growth. These results suggest that HB might be effective in preventing sarcopenia associated with obesity.
Barré Syndrome - ecovery from Guillain and Regulated Cytokines Correlates with R Expression of Early Growth Response Gene-2

Guillain–Barre´ syndrome (GBS) is an immune-mediated peripheral neuropathy. The goal of this research was the identiﬁcation of biomarkers associated with recovery from GBS. In this study, we compared the transcriptome of PBMCs from a GBS patient and her healthy twin to discover possible correlates of disease progression and recovery. The study was then extended using GBS and spinal cord injury unrelated patients with similar medications and healthy individuals. The early growth response gene-2 (EGR2) was upregulated in GBS patients during disease recovery. The results provided evidence for the implication of EGR2 in GBS and suggested a role for EGR2 in the regulation of IL-17, IL-22, IL-28A, and TNF- b cytokines in GBS patients. These results identiﬁed biomarkers associated with GBS recovery and suggested that EGR2 overexpression has a pivotal role in the downregulation of cytokines implicated in the pathophysiology of this acute neuropathy. The Journal of Immunology , 2016, 196: 000–000.
SSICC: Sharing Sensitive Information in a Cloud-of-Clouds

—The need to share and manipulate sensitive data is a challenge for most content providers using the cloud for storage. The objective of this work is to propose an architecture to ensure a distributed access control to secure sharing of sensitive electronic documents in the cloud. This paper will explain the architecture of the model, details of the protocols, implementation, and analysis on security, usability, and performance. The main features of the proposed model are: the use of Identity-Based Encryption, byzantine fault tolerance, reliable integrity check, secure user revocation, low complexity in the management of cryptographic keys and secure sharing of sensitive electronic documents.
Potency Differences for D -Phe-Cys-Tyr- D -Trp-Arg-Thr-Pen-Thr-NH 2 as an Antagonist of Peptide and Alkaloid (cid:1) -Agonists in an Antinociception Assay

D -Phe-Cys-Tyr- D -Trp-Arg-Thr-Pen-Thr-NH 2 (CTAP) is a pep- tide antagonist that demonstrates potent and selective affinity for (cid:1) -opioid receptors in radioligand binding assays and in vitro bioassays. However, previous studies indicate that CTAP may possess unusual pharmacology under certain conditions. Therefore, CTAP was evaluated as an antagonist of the antino- ciceptive effects of a range of structurally diverse high- and low-efficacy peptide and alkaloid opioid agonists and com- pared with the traditional antagonist naltrexone. Male Sprague-Dawley rats ( N (cid:1) 227) were loosely restrained and the latency for tail withdrawal from 55°C water was measured. Morphine s.c. and i.c. v. , buprenorphine s.c. , etorphine s.c. and i.c. v. , [ N -Me-Phe 3 , D -Pro 4 ]-morphiceptin and [ D -Ala 2 , N -Me-Phe 4 ,Gly 5 -ol]-enkephalin produced antinociceptive effects. CTAP was at least 10-fold more potent than naltrexone as an antagonist of the antinociceptive effects of all five agonists. High doses of CTAP produced a noncompetitive antagonism of etorphine s.c. and morphine s.c. suggesting that CTAP may interact with additional