Miracle-dz/UniFAR-FTUs huggingface dataset:
Pulmonary dendritic cells and CD8 T cells facilitate protection following influenza A virus vaccination and infection

Pulmonary dendritic cells and CD8 T cells facilitate protection following influenza A virus vaccination and infection. ii To my parents for their unending support iii Science and everyday life cannot and should not be separated. Science, for me, gives a partial explanation for life. In so far as it goes, it is based on fact, experience and experiment. Rosalind Franklin Letter to Ellis Franklin iv ACKNOWLEDGMENTS There are many people I would like to thank; without whom, the completion of this dissertation would not have been possible. I would first like to thank my advisor, Dr. Kevin Legge, for his patience, encouragement and guidance during my time in his laboratory. I would also like to thank the members of my dissertation committee; Dr. for their input and direction throughout this process. Further, I would like to thank the members of the Legge lab not only for their technical assistance, but also for their theoretical insight throughout the development of my project. I would specifically like to thank Legge lab members Dr. Ryan Langlois and Emma Hornick for their invaluable support; this endeavor would certainly not have been as successful or enjoyable without them. The studies presented within this dissertation could not have been completed without assistance and reagents from a number of individuals in the Department of Pathology, the Interdisciplinary Graduate Program in Immunology and at other institutions. In this regard, I would especially like to thank Dr. for their constant reassurance and understanding throughout the completion of my dissertation. v ABSTRACT The severe disease associated with seasonal epidemics of influenza A virus (IAV), as well as pandemic outbreaks, have highlighted the necessity for novel, broadly cross-reactive vaccination and therapeutic strategies against IAV. Our studies have focused on the contribution of IAV-specific CD8 T cells to mediating protection following IAV vaccination and infection as IAV-specific CD8 T cells are required for clearance of IAV. Further, IAV-specific CD8 T cells are typically cross-protective as they are generally directed at highly conserved areas of IAV. Recently, influenza virus-like particles (VLPs) have been developed from recombinant baculoviruses containing influenza proteins hemagglutinin (HA) and/or neuraminidase (NA) on the surface and matrix (M1) in the VLP core. Influenza VLPs induce potent antibody responses and have been shown to provide protection from morbidity and mortality during lethal homo-and hetero-subtypic IAV challenge. This suggests that conserved, VLP-induced CD8 T cell responses may also contribute to the overall …
Incidence of mastitis in Sahiwal cattle and Murrah buffaloes of a closed organized herd

The incidence of mastitis was investigated in Sahiwal and Murrah breeds maintained as a closed pedigreed herd in a research institute. The overall occurrence of mastitis in Sahiwal and Murrah was 26.43 and 18.91%, respectively. Least squares analysis revealed that breed, season of calving and milk yield had significant effect on the incidence of mastitis. The incidence was the highest among cows calved in summer, whereas it was the lowest in winter calvers in Sahiwal and autumn calvers in Murrah. High milk producers had significantly higher incidence than their low yielding counterparts. There was a nonsignificant trend of increase in incidence with the increase of parity order.
Effect of Withania somnifera on chlorpyrifos induced neurotoxicity in rats.

Withania somnifera, a medicinal herb having potential antioxidant activity, has been explored to ameliorate chlorpyrifos induced neurotoxicity in adult Wistar rats. The root extract of the plant was given i/p @ 25 mg/kg and 50 mg/kg daily for 14 days after i/p administration of chlorpyrifos @ 85 mg/kg. This study indicated no beneficial effect of the plant extract at given doses on chlorpyrifos-induced neurobehavioral alterations or on brain and plasma acetylcholinesterase inhibition. It rather aggravated these effects.
PERBAIKAN FASILITAS KERJA UNTUK MENGURANGI KELUHAN MUSKULOSKELETAL

Hand embroidery worker is one of the forms of informal sectors in Nagari Koto Gadang, Sumatera Barat which has not yet applied an ergonomic working facilities in its daily activity. There were 84% worker who complain of pain during work.The purpose of this experimental study with pretest-posttest with control group design was to analyze the influence of intervention of working facilities work posture to reduce musculoskeletal disorder in the hand embroidery workers in Nagari Koto Gadang, Sumatera Barat. The populations of this study was 36 embroidery workers and 26 of them were selected to be samples for this study. The technique of sampling was total sampling, where there were 10 respondents who do not meet the criteria of inclusion.The result of this research, before the working facility intervention, their work posture was on the high and medium levels. After working facility intervention, their work posture was improved to small level. The result of Wilcoxon test showed there were difference musculoskeletal disorder working (p=0.01)in the experiment group before and after working facility intervention. The hand embroidery workers are suggested to use the working facilities which have been ergonomically design, to do some relaxation when they are working, it’s needed to make an adjustable work (pamedangan) and using a lumbar brace to minimize the musculoskeletal disorder. Perajin sulaman tangan merupakan salah satu bentuk usaha sektor informal di Nagari Koto Gadang Sumatera Barat yang belum menerapkan fasilitas kerja(pamedangan) yang ergonomis dalam bekerja.Terdapat 84% perajin yang mengeluh sakit pinggang selama bekerja. Penelitian ini bertujuan untuk menganalisis pengaruh perbaikan fasilitas kerja untuk mengurangi keluhan muskuloskeletal pada perajin sulamantangan di Nagari Koto Gadang Sumatera Barat. Populasi dari penelitian ini adalah sebanyak 36 orang dengan jumlah sampel sebanyak 26 orang. Teknik pengambilan sampel yaitu total sampling, dimana terdapat 10 orang responden yang tidak memenuhi kriteria inklusi. Jenis penelitian ini adalah penelitian eksperimen sungguhan dengan bentuk rancangan Pretest – Postestwith Control Group. Hasil penelitian yang diperoleh yaitu sikap kerja sebelum intervensi fasilitaskerja berada pada level tindakan tinggi dan sedang. Sesudah intervensi fasilitas kerja,terjadi perbaikan sikap kerja menjadi level tindakan menjadi kecil. Hasil ujiWilcoxon menunjukkan adanya pengaruh perbedaan keluhanmuskuloskeletal pada saat bekerja (p=0,01)pada kelompok perlakuan sebelum dan sesudah intervensi fasilitas kerja. Perajin sulaman tangan disarankan agar dapat menggunakan fasilitas kerjayang telah didesain secara ergonomis, melakukan relaksasi selama bekerja, perlunyamembuat meja kerja (pamedangan) yang adjustable dan penahan lumbar agar dapatmengurangi keluhan muskoloskeletal.
Anemia in Hospitalized Patients: A Cross-Sectional Study on Different Erythrocyte Indices and their Relationships

Background: The prevalence of anemia varies according to age, gender and ethnicity. Moreover, its occurrence in the significant proportions of hospitalized patients is a matter of concern for the healthcare practitioners. Objective: The objective of the study was to determine the prevalence of anemia in hospitalized patients in relation to age, gender, and ethnicity and to assess the relationships of different erythrocyte indices with hemoglobin. Materials and Methods: In a hospital-based, cross-sectional study, the hematological test results of 485 patients were analyzed. Participants were categorized according to gender, age and ethnicities and diagnosed as anemic based on hemoglobin levels <12.0 g/dL in females and <13.0 g/dL in males. Erythrocyte indices like hemoglobin, erythrocyte count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were compared between the anemic and normal participants apart from different subgroups. Results: The prevalence of anemia in the hospitalized patients was 65.8%. It was greater in females as compared to males. Likewise, it was the highest in the highest quartile of age and Indo-Aryan ethnic group. Hemoglobin concentration was significantly lower in the female participants than in the males (p<0.001); significantly different across different age groups (quartile of age) (p<0.001). Hemoglobin was found to be significantly and positively correlated with erythrocyte count, hematocrit, mean corpuscular volume and mean corpuscular hemoglobin (p<0.001). Hematocrit followed by erythrocyte count were the most efficient predictors of the diagnosis of anemia. Moreover, RBC count/HCT with MCV and MCH together could significantly explain the variance in hemoglobin levels, after controlling for age and gender (p<0.001). Conclusion: Anemia was found to be significantly prevalent in hospitalized patients. Moreover, the erythrocyte indices like erythrocyte count, hematocrit and hemoglobin concentration were effective in characterizing anemia.
Status of reproductive performance in rural buffaloes artificially inseminated using deep frozen semen

Data on 6 762 artificral inserminations on 5 793 village buffaloes belonging to 2 650 farmers spread over 17 villages in Maharastra during June 1991 to September 1996 were analysed for studying reproductive performance in rural buffaloes artifucailly inseminated useing deep frozen semen. The average farmer herd size was of 2.19. The mean conception rate was observed to be 51. 84 +- 0.61%. The local buffalo breeds (Pandharpuri and Nagpuri) exhibited higher conception rate than Murrah or Mehsana breeds. Generally conception rate improved from first lactation (39.36+- 1.61%) to third lactation (56.25+-1.67%) and then declined. Parity, season of insemination, uterine horn tone and AI sequence had significant effect on conception rate. Presence of mature follicle on either right or left ovary, type of vaginal discharge and eve the thawing temperature of water did not affect the extent of conception rate. It was recommended that among other factors, farmer observation on vaginal dischage , vulva swelling, bellowing can be relied upon for improving conception rate.
An engineered minimal chromosomal passenger complex reveals a role for INC ENP/Sli15 spindle association in chromosome biorientation

kinase-activating Sli15/ INC ENP scaffold and the catalytic subunit Ipl1/Aurora B. Although native Sli15 is not oligomeric, artificial dimerization suppressed the biorientation defect and lethality associated with deletion of a majority of its microtubule-binding domain. Dimerization did not act through a physical clustering-based kinase activation mechanism but instead promoted spindle association, likely via a putative helical domain in Sli15 that is essential even when dimerized and is required to target kinetochore substrates. Based on the engineering and characterization of a minimal CPC, we suggest that spindle association is important for active Ipl1/Aurora B complexes to preferentially destabilize misattached kinetochores.
Shared decision making during antenatal counselling for anticipated extremely preterm birth.

Objectives To explore health care providers' (HCPs) perceptions of using shared decision making (SDM) and to identify facilitators of and barriers to its use with families facing the anticipated birth of an extremely preterm infant at 22+0 to 25+6 weeks gestational age. Study Design Qualitative descriptive study design: we conducted interviews with 25 HCPs involved in five cases at a tertiary care centre and completed qualitative content analysis of their responses. Results Nine facilitators and 16 barriers were identified. Facilitators included: a correct understanding of this process and how to apply it, a belief that parents should be the decision makers in these situations, and a positive outlook toward using SDM during antenatal counselling. Barriers included: HCPs' misunderstandings of how and when to apply SDM during antenatal counselling, challenges using the process for cases at the lower end of the gestational age range, fear of the negative emotions and stress parents face when making decisions, and HCPs' uncertainty about their ability to properly apply SDM. Conclusions This study identified facilitators and barriers to use of SDM during antenatal counselling for anticipated birth of extremely preterm infants that can be used to inform development of tailored strategies to facilitate future implementation of shared decision making in this area.
Does the British Heart Foundation PocketCPR training application improve confidence in bystanders performing CPR?

Objectives: Out-of-hospital cardiac arrest has poor prognosis and patients rarely survive unless they receive immediate cardiopulmonary resuscitation from bystanders. In 2012, the British Heart Foundation launched its PocketCPR training application to simplify bystander cardiopulmonary resuscitation training and overcome barriers to resuscitation. This study investigates whether the British Heart Foundation PocketCPR training application improves the confidence of bystanders who perform cardiopulmonary resuscitation during simulated resuscitation attempts. Methods: This is a mixed method study using a randomised crossover trial with questionnaire analysis. One hundred and twenty participants were randomised to either perform two minutes of cardiopulmonary resuscitation on a resuscitation manikin using the British Heart Foundation PocketCPR application or perform cardiopulmonary resuscitation without instruction. Participants completed a questionnaire to capture their confidence before completing the opposite arm of the study. Each participant then completed a second questionnaire to allow for comparison of levels of confidence. Results: Participants in this study were more confident in their overall performance of cardio-pulmonary resuscitation using the British Heart Foundation PocketCPR training application compared to performing cardiopulmonary resuscitation without instruction (mean confidence score (0–100): 50.41 with PocketCPR and 43.92 without (p = 0.026)). They were also more confident that the number of chest compressions in this study was correct (mean: 60.39 with PocketCPR vs. 46.10 without (p < 0.001)), and in the delivery of cardiopulmonary resuscitation without having recent cardiopulmonary resuscitation training (mean: 48.67 with PocketCPR vs. 39.79 without (p < 0.002)). Conclusion: The British Heart Foundation PocketCPR training application improved the confidence of bystanders performing cardiopulmonary resuscitation during simulated resuscitation attempts.
Renal function in β-thalassemia major receiving desferal versus deferasirox

Introduction: Deferasirox is a new oral iron chelating agent which has been administered in β-thalassemia major patients in last few years. There is some reports regarding nephrotoxicity of this agent; however, no comparative study has been conducted yet. Objectives: The aim of this study was to compare the prevalence of kidney dysfunction in β-thalassemia major patients receiving either desferal or deferasirox as iron chelating agents. Patients and Methods: In this cross-sectional study, adult patients with β-thalassemia major who received 25 mg/kg/d of desferal or 25 mg/kg/d of deferasirox were studied. We compared them for serum calcium (Ca), creatinine (Cr) levels and 24 hours urine collection for proportion of Ca and protein. Estimated glomerular filtration rate (eGFR) was calculated by Cockcroft-Gault formula. Results: Twenty-seven patients receiving desferal and 23 patients receiving deferasirox were evaluated. There was no significant difference of calciuria (P=0.19), glycosuria (P=0.508), mean 24-hour urine proteinuria (P=0.44), mean serum Cr (P=0.47), serum Ca level (P = 0.067) and mean eGFR (P=0.42) between two groups. Conclusion: There is no significant difference of hypercalciuria, glycosuria, mean eGFR, proteinuria, and also serum Cr between β-thalassemia major patients who received desferal or deferasirox.
Integration of Geoelectrical and Geotechnical Data for Soil Characterization in Parts of Owerri Metropolis Southeastern Nigeria

The increasing rate of building and construction projects in Owerri Metropolis given the topographic nature has necessitated proper evaluation of the foundation beds in relation to elevation, to assess the suitability of the soil for engineering structures. Consequently, an integrated geoelectric and geotechnical approach of investigating soil characteristics was adopted. Ten Vertical Electrical Soundings (VES) were carried out using the OHMEGA-500 resistivity metre to acquire data which were analysed using the advanced Geosciences Incorporation (AGI) 1D Software package. At each VES location, elevation and coordinates were measured and soil samples collected to a depth of 2m were grouped to form three composite samples according to the elevation of the study area namely high, medium and low elevations. The samples were then subjected to laboratory tests for geotechnical parameters. The topsoil is composed of sand and laterite with resistivity ranging from 41. 6Ωm to 7532Ωm and thickness of 3.2m. Elevation ranges from 140ft to 450ft. Geotechnical results revealed moisture content of 11.33 to 14.33%, liquid limit of 19.2 to 22.8 and plastic index (PI) of zero, showing none plastic soil. Grain size analysis revealed medium-dense sand with negligible gravel or silt. Compaction test result gave Maximum Dry Density (MDD) and Optimum Moisture Content (OMC) of 1.8 to 1.99 and 13 to 15% respectively. Triaxial test revealed the angle of internal friction of 18 to 27with cohesion values ranging from 20KN/mto 49KN/m indicating high strength to shearing and high tendency to withstand load. All measured parameters fall within recommended standards in spite of variation in elevation.
The Long Non-Coding RNA MALAT1 Enhances Ovarian Cancer Cell Stemness by Inhibiting YAP Translocation from Nucleus to Cytoplasm

Background The purpose of this work was to unearth the effects and underlying mechanism of long non-coding RNA (lncRNA) MALAT1 in ovarian cancer cell stemness. Material/Methods Western blot, quantitative polymerase chain reaction (qPCR) and sphere forming analysis were performed to evaluate the stem-like traits of cells and MALAT1-induced effects on ovarian cancer cell stemness. Cell viability was performed to evaluate MALAT1 role in the chemoresistance of ovarian cancer cells. RNA immunoprecipitation (RIP) and luciferase reporter analysis were constructed to investigate the underlying mechanisms. Results Here, qPCR assay showed that MALAT1 level was remarkably higher in non-adherent spheres formed by adherent ovarian cancer cells, as well as cisplatin-resistant ovarian cancer cells. Additionally, MALAT1 knockdown reduced ovarian cancer cell stemness, characterized as the decrease of sphere forming ability, expression of stemness regulatory masters, and attenuation of cisplatin resistance. Moreover, MALAT1 interacted with yes-associated protein (YAP), inhibited its nuclear-cytoplasm translocation, promoted YAP protein stability and expression and thus increased its activity. Notably, rescuing expression of YAP attenuated the inhibition of MALAT1 knockdown on ovarian cancer cell stemness. Conclusions In conclusion, these results demonstrate a MALAT1/YAP axis responsible for ovarian cancer cell stemness.
Skeletal, dentoalveolar, and periodontal changes of skeletally matured patients with maxillary deficiency treated with microimplant-assisted rapid palatal expansion appliances: A pilot study

Introduction: Microimplant-assisted rapid palatal expansion (MARPE) has recently been offered to adult patients for correcting maxillary transverse deficiency. However, there is limited information in the literature on the success of this appliance and its skeletal and dental effects on skeletally matured patients. The purpose of this study was to investigate the immediate skeletal, dentoalveolar, and periodontal response to MARPE appliance using cone-beam computed tomography in a skeletally matured patient as assessed by the cervical vertebral maturation method. Materials and Methods: Eight consecutively treated patients (2 females, 6 males; mean age of 21.9 ± 1.5 years) treated with a maxillary skeletal expander were included in the study. Measurements were taken before and after expansion to determine the amount of midpalatal suture opening, upper facial bony expansion, alveolar bone bending, dental tipping, and buccal bone thickness (BBT). Data were analyzed using a one-way ANOVA and matched-pair t-test (α = 0.05). Results: Midpalatal suture separation was found in 100% of the patients with no dislodged microimplants. Total maxillary expansion was attributed to 41% skeletal, 12% alveolar bone bending, and 48% dental tipping. Pattern of midpalatal suture opening was found to be parallel in both the coronal and axial planes. On average, the absolute dental tipping ranged from 4.17° to 4.96° and the BBT was reduced by an average of 39% measured at the premolars and molars. Conclusions: The MARPE appliance can be a clinically acceptable, nonsurgical treatment option for correcting mild to moderate maxillary transverse discrepancies for skeletally matured adult patients with a healthy periodontium.
UvA-DARE (Digital Academic Repository) Dyslexia and voxel-based morphometry: correlations between five behavioural measures of dyslexia and gray and white matter volumes Tamboer,

In voxel-based morphometry studies of dyslexia, the relation between causal theories of dyslexia and gray matter (GM) and white matter (WM) volume alterations is still under debate. Some alterations are consistently reported, but others failed to reach significance. We investigated GM alterations in a large sample of Dutch students (37 dyslexics and 57 nondyslexics) with two analyses: group differences in local GM and total GM and WM volume and correlations between GM and WM volumes and five behavioural measures. We found no significant group differences after corrections for multiple comparisons although total WM volume was lower in the group of dyslexics when age was partialled out. We presented an overview of uncorrected clusters of voxels (p<0.05, cluster size k>200) with reduced or increased GM volume. We found four significant correlations between factors of dyslexia representing various behavioural measures and the clusters found in the first analysis. In the whole sample, a factor related to performances in spelling correlated negatively with GM volume in the left posterior cerebellum. Within the group of dyslexics, a factor related to performances in Dutch–English rhyme words correlated positively with GM volume in the left and right caudate nucleus and negatively with increased total WM volume. Most of our findings were in accordance with previous reports. A relatively new finding was the involvement of the caudate nucleus. We confirmed the multiple cognitive nature of dyslexia and suggested that experience greatly influences anatomical alterations depending on various subtypes of dyslexia, especially in a student sample.