Miracle-dz/UniFAR-FTUs huggingface dataset:
Review of Technological Challenges in Personalised Medicine and Early Diagnosis of Neurodegenerative Disorders

Neurodegenerative disorders are characterised by progressive neuron loss in specific brain areas. The most common are Alzheimer’s disease and Parkinson’s disease; in both cases, diagnosis is based on clinical tests with limited capability to discriminate between similar neurodegenerative disorders and detect the early stages of the disease. It is common that by the time a patient is diagnosed with the disease, the level of neurodegeneration is already severe. Thus, it is critical to find new diagnostic methods that allow earlier and more accurate disease detection. This study reviews the methods available for the clinical diagnosis of neurodegenerative diseases and potentially interesting new technologies. Neuroimaging techniques are the most widely used in clinical practice, and new techniques such as magnetic resonance imaging (MRI) and positron emission tomography (PET) have significantly improved the diagnosis quality. Identifying biomarkers in peripheral samples such as blood or cerebrospinal fluid is a major focus of the current research on neurodegenerative diseases. The discovery of good markers could allow preventive screening to identify early or asymptomatic stages of the neurodegenerative process. These methods, in combination with artificial intelligence, could contribute to the generation of predictive models that will help clinicians in the early diagnosis, stratification, and prognostic assessment of patients, leading to improvements in patient treatment and quality of life.
Integrated metabolomic and transcriptomic analysis reveal the effect of mechanical stress on sugar metabolism in tea leaves (Camellia sinensis) post-harvest

Sugar metabolites not only act as the key compounds in tea plant response to stress but are also critical for tea quality formation during the post-harvest processing of tea leaves. However, the mechanisms by which sugar metabolites in post-harvest tea leaves respond to mechanical stress are unclear. In this study, we aimed to investigate the effects of mechanical stress on saccharide metabolites and related post-harvest tea genes. Withered (C15) and mechanically-stressed (V15) for 15 min Oolong tea leaves were used for metabolome and transcriptome sequencing analyses. We identified a total of 19 sugar metabolites, most of which increased in C15 and V15. A total of 69 genes related to sugar metabolism were identified using transcriptome analysis, most of which were down-regulated in C15 and V15. To further understand the relationship between the down-regulated genes and sugar metabolites, we analyzed the sucrose and starch, galactose, and glycolysis metabolic pathways, and found that several key genes of invertase (INV), α-amylase (AMY), β-amylase (BMY), aldose 1-epimerase (AEP), and α-galactosidase (AGAL) were down-regulated. This inhibited the hydrolysis of sugars and might have contributed to the enrichment of galactose and D-mannose in V15. Additionally, galactinol synthase (Gols), raffinose synthase (RS), hexokinase (HXK), 6-phosphofructokinase 1 (PFK-1), and pyruvate kinase (PK) genes were significantly upregulated in V15, promoting the accumulation of D-fructose-6-phosphate (D-Fru-6P), D-glucose-6-phosphate (D-glu-6P), and D-glucose. Transcriptome and metabolome association analysis showed that the glycolysis pathway was enhanced and the hydrolysis rate of sugars related to hemicellulose synthesis slowed in response to mechanical stress. In this study, we explored the role of sugar in the response of post-harvest tea leaves to mechanical stress by analyzing differences in the expression of sugar metabolites and related genes. Our results improve the understanding of post-harvest tea’s resistance to mechanical stress and the associated mechanism of sugar metabolism. The resulting treatment may be used to control the quality of Oolong tea.
Immersive Video Postprocessing for Efficient Video Coding

This paper describes two methods for increasing the efficiency of the MPEG Immersive Video (MIV) coding standard. The methods manipulate the MIV-formatted atlas videos by considering the coding principles of the widely used video encoders, enhancing the encoding efficiency for the immersive video content. The first method, patch average color modification removes the constant component of all YCbCr components of each patch within atlases, resulting in the reduction of the number and magnitude of edges within a texture atlas video. The second proposed method changes the dynamic range of the geometry (depth) atlas, adapting to the quality of input depth maps. Both methods proposed by the authors of this paper were included into the Test Model for MPEG Immersive Video (TMIV), which is the reference implementation of the MIV codec. Moreover, the metadata syntax relating to the first proposed method was adopted to the ISO/IEC 23090–12 standard.
A comparison of computed tomography imaging with histopathology in the sensitivity and correlation of evaluating coronary arterial calcification

Background The sensitivity and correlation of coronary computed tomography angiography (CTA) as compared with histopathology are unknown in evaluating coronary arterial calcification. In this study, we retrospectively evaluated qualitatively and quantitatively the sensitivity and correlation of coronary CTA compared with histopathology in assessing coronary arterial calcification. Methods This study was conducted on 12 randomly selected cadavers aged over 40 years at the time of death, and 53 segments of coronary arteries from these 12 cadavers were obtained from the Human Anatomy Laboratory of Tianjin Medical University. The artery segments were scanned using contrasted-enhanced dual-source computed tomography (DSCT) with an axial slice thickness of 0.6 mm. Coronary artery calcification in a coronary segment was defined as the presence of 1 or more voxels with a CT density >130 Hounsfield units. According to the arc of calcification in the cross section of the coronary artery wall, calcified plaques were divided into three categories: mild, moderate, and severe calcification. The coronary artery stenosis caused by calcified plaque was observed and calculated with multiplanar reconstruction (MPR), maximum density projection, volume rendering (VR), and cross-sectional reconstruction. After CT enhancement scanning, the coronary artery specimens were cut into 4-mm long segments and embedded in paraffin for pathological staining. Pathological classification and coronary artery stenosis measured with pathological analysis were used as comparison criteria. Results Histopathology detected 69 Vb-type plaques, while DSCT detected 57 calcified plaques. The sensitivity of CT for detecting mild, moderate, and severe calcified plaques were 88.3% [95% confidence interval (CI): 74.1–95.6%], 100% (95% CI: 69.8–100%), and 100% (95% CI: 73.2–100%), respectively. DSCT had a significant (P<0.001) correlation with histopathology in quantifying coronary artery stenosis caused by mild, moderate, and severe calcified plaques (R2=0.9278, R2=0.9158, R2=0.7923, respectively). Compared with histopathology, DSCT overestimated coronary artery stenosis caused by mild, moderate, and severe calcified plaques (3.2%±2.0%, 4.9%±4.7%, and 14.7%±8.2%, respectively; P<0.05). Conclusions DSCT contrast enhancement scanning can detect and characterize coronary artery calcification with a good correlation with histopathologic quantification of coronary artery stenosis caused by different types of calcified plaques, even though coronary CTA may overestimate the stenosis.
A systematic review of empirical studies on trust between universities and society

ABSTRACT The perception of universities as trustworthy institutions has been challenged by heightened public distrust in science and public institutions. In this context, understanding trust relationships between higher education institutions and society could provide vital intelligence to safeguard and grow the increasingly disrupted higher education sector. The article presents a systematic review of empirical studies that focus on trust in higher education institutions. It aims to map research trends and empirical gaps to provide insights into the state of research in this area. The review found that most research has examined trust within the university context, with significantly less attention on trust between the universities and the broader societies they serve. Within the latter, trust relationships with business and industry domains have received greater empirical attention than those with community and government. These findings have implications for purposeful and meaningful trust-based capacity and capability building at individual and institutional levels.
Determinants of Parents Taking Their Children for Scheduled Vaccinations during COVID-19 Pandemic in South Africa

Scheduled or routine childhood vaccinations are known for their effectiveness in eradicating fear for many life-threatening and disabling diseases and saving lives globally. This paper is aimed at assessing determinants of parents taking their children for scheduled vaccinations during the COVID-19 pandemic in South Africa. Data used for this paper were obtained from the Human Sciences Research Council’s (HSRC) COVID-19 Online Survey titled “One Year Later Survey”, which was conducted between 25 June and 11 October 2021 in South Africa. Multivariate logistic regression analysis was performed to achieve this study goal. Findings showed that just over half of parents (56.7%) reported taking their children for scheduled vaccinations across the country. Males were significantly less likely (aOR = 0.53 95% CI [0.45–0.61], p < 0.001) to have taken their children for scheduled vaccinations than females. Parents’ experiences and views were among key determinants of parents having taken their children for scheduled vaccinations in South Africa. Parents who had never taken influenza (flu) vaccines were significantly less likely (aOR = 0.33 [0.28–039], p < 0.001) to have taken their children for scheduled vaccinations than those who had taken flu vaccines. Parents who did not know anyone who had personally experienced serious side effects to any vaccine were significantly less likely (aOR = 0.77 [0.66–0.90], p = 0.001) to have taken their children for scheduled vaccinations than those who knew anyone who had experienced them. Parents who did not think vaccines were a good way to protect communities from disease were significantly less likely (aOR = 0.50 [0.33–0.77], p = 0.001) to have taken their children for scheduled vaccinations than those who thought vaccines were a good way to protect communities from disease. These findings are of significance especially during the time when the country is still struggling to reach a substantial proportion of its population vaccinated for COVID-19. Thus, these findings may be relevant in determining parents’ intentions to have their children receive the South African Department of Health recommended vaccines for their respective age group.
Symmetry Breaking and Bifurcations in the Periodic Orbit Theory. I

We derive an analytical trace formula for the level density of two-dimensional elliptic billiards using an improved stationary phase method. The result is a continuous function of the deformation parameter (eccentricity) through all bifurcation points of the short diameter orbit and its repetitions, and possesses the correct limit of circular billiard at zero eccentricity. Away from the circular limit and the bifurcations, it reduces to the usual (extended) Gutzwiller trace formula, which for the leading-order families of periodic orbits is identical to the result of Berry and Tabor. We show that the circular disk limit of the diameter-orbit contribution is also reached through contributions from closed (periodic and non-periodic) orbits of the hyperbolic type with an even number of reflections from the boundary. We obtain the Maslov indices depending on deformation and energy in terms of the phases of the complex error and Airy functions. We find enhancement of the amplitudes near the common bifurcation points of short-diameter and hyperbolic orbits. The calculated semiclassical level densities and shell energies are in good agreement with the quantum mechanical ones.
Heat Transfer in a Short Parallel-Plate Channel in the Transition Regime

Heat transfer in a parallel-plate channel with uniform wall heat flux and refrigerated by water is investigated experimentally for the laminar and transition hydrodynamic regimes. The dimensions of the channel were chosen such that entry effects are relevant for a significant part of the channel length. The influence of the following parameters has been studied: inlet Reynolds number, inlet temperature and wall heat flux. Experimental results are compared with predictions based on different empirical correlations for convection in ducts. This analysis shows that the correlation of Gnielinski provides a good agreement with the experimental results for Reynolds numbers between 5,000 and 10,000 at 20°C and under relatively low heat fluxes. Experiments performed at relatively low heat fluxes show that, in the entry region previous to the laminar-turbulent transition, the behavior of the flow is well described by the combined hydrodynamic and thermal entry solution obtained by Hwang and Fan (Finite Difference Analysis of Forced-Convection Heat Transfer in Inlet Region of a Flat Rectangular Duct, Appl. Sci. Res., vol. 13, pp. 401–422, 1964). The hydrodynamic and thermal entry effects for the experimental conditions in the present work are relevant for most of the test section. The location of the transition point is affected in the first instance by the local bulk Reynolds. On the other hand, the wall heat flux affects the fluid properties, i.e., the fluid viscosity, and therefore, it also affects the transition. It was found that for the transition regime, an increase of the wall heat flux produces lower values of the convection coefficient than the values predicted by the correlation of Gnielinski. This behavior, which seems to be associated to the variation of the fluid properties, is opposite to the increase of the convection coefficient observed for heating in the laminar and turbulent regimes for water and oils. The case of an inlet Reynolds number of 5,000 was studied in detail, and the influence of heat flux on the Nusselt number is addressed.
Detection and Identification of Mycoplasmopsis agassizii in Captive Tortoises with Different Clinical Signs in Italy

Simple Summary Some species of turtle and tortoise are vulnerable or declared threatened and pathogens such as Mycoplasmopsis agassizii can cause severe declines in their populations. Diagnosis and control of transmissible disease is the key for limiting the spread of pathogens in collection of animals. Sequencing and sharing sequences of mycoplasmas obtained from reptiles is important to improve diagnostic tests and to monitor the species affinity and the cross-species transmission potential of mycoplasmas. This study stresses the important role of practitioners in proper collection and sharing of data for appropriate analysis. The results obtained show that M. agasizii is detectable in different specimens and how is important to collect the proper sample to avoid false negative results. Not only animals with upper respiratory diseases should be considered as potentially infected, because M. agassizii sequences were found also in asymptomatic animals and in tortoises with stomatitis without respiratory signs. Coinfection with or infection due to other pathogens should be also considered. Abstract Mycoplasmopsis agassizii causes the Upper Respiratory Tract Disease (URTD) in tortoises. The severity of the disease usually ranges from mild to severe respiratory signs. Animals can recover, die, or become asymptomatic carriers and are source of infection for other tortoises. This study describes (i) the clinical history and the results obtained in ten years of diagnostic PCR activity for detecting M. agassizii in different species of captive tortoises in Italy, and (ii) the phylogenetic analysis of the 16S rRNA gene sequences of M. agassizii. A total of 26.0% out of 169 samples resulted positive by PCR and 32 out of 75 (42.7%) animals with symptoms were positive. Sequences ob-tained from the PCR products were conserved, differed from the sequence of the M. agassizii type strain PS6, and were identical to many M. agassizii sequences deposited in databases. In particular, the sequences were identical or very similar to sequences obtained previously from tortoises in It-aly. Since samples collected from different anatomical sites resulted positive, it is suggested that pools of conjunctival, nasal and oral swabs are tested for diagnostic purpose in both symptomatic and asymptomatic animals.
Cardiotoxicity of Electronic Cigarettes and Heat-Not-Burn Tobacco Products—A Problem for the Modern Pediatric Cardiologist

Electronic nicotine delivery systems (ENDS) have become increasingly popular among adolescents, either as an alternative to conventional cigarettes (CCs) or as a newly acquired recreational habit. Although considered by most users as a safer option for nicotine intake, these devices pose significant health risks, resulting in multisystem damage. Heat-not-burn products, which, unlike ENDS, contain tobacco, are also alternatives to CCs that consumers use based on the idea that their safety profile is superior to that of cigarettes. Recent studies in the USA and EU show that adolescents are particularly prone to using these devices. Pediatric cardiologists, as well as other healthcare professionals, should be aware of the complications that may arise from acute and chronic consumption of these substances, considering the cardiovascular damage they elicit. This article summarized the known data about the impact of ENDS on the cardiovascular system, with emphasis on the pathophysiological and molecular changes that herald the onset of systemic lesions alongside the clinical cardiovascular manifestations in this scenario.
Vapor Phase Ammonia Curing to Improve the Mechanical Properties of Antireflection Optical Coatings Designed for Power Laser Optics

Projects of inertial confinement fusion using lasers need numerous optical components whose coatings allow the increase in their transmission and their resistance to high laser fluence. A coating process based on the self-assembly of sol–gel silica nanoparticles and a post-treatment with ammonia vapor over the surfaces of the optical components (“ammonia curing process”) was developed and successfully optimized for industrial production. Manufacturing such antireflective coatings has clear advantages: (i) it is much cheaper than conventional top-down processes; (ii) it is well adapted to large-sized optical components and large-scale production; and (iii) it gives low optical losses in transmission and high resistances to laser fluence. The post-treatment was achieved by a simple exposition of optical components to room-temperature ammonia vapors. The resulting curing process induced strong optical and mechanical changes at the interface and was revealed to be of paramount importance since it reinforced the adhesion and abrasion resistance of the components so that the optical components could be handled easily. Here, we discuss how such coatings were characterized and how the initial thin nanoparticle film was transformed from a brittle film to a resistant coating from the ammonia curing process.
Type I Diabetes Pathoetiology and Pathophysiology: Roles of the Gut Microbiome, Pancreatic Cellular Interactions, and the ‘Bystander’ Activation of Memory CD8+ T Cells

Type 1 diabetes mellitus (T1DM) arises from the failure of pancreatic β-cells to produce adequate insulin, usually as a consequence of extensive pancreatic β-cell destruction. T1DM is classed as an immune-mediated condition. However, the processes that drive pancreatic β-cell apoptosis remain to be determined, resulting in a failure to prevent ongoing cellular destruction. Alteration in mitochondrial function is clearly the major pathophysiological process underpinning pancreatic β-cell loss in T1DM. As with many medical conditions, there is a growing interest in T1DM as to the role of the gut microbiome, including the interactions of gut bacteria with Candida albicans fungal infection. Gut dysbiosis and gut permeability are intimately associated with raised levels of circulating lipopolysaccharide and suppressed butyrate levels, which can act to dysregulate immune responses and systemic mitochondrial function. This manuscript reviews broad bodies of data on T1DM pathophysiology, highlighting the importance of alterations in the mitochondrial melatonergic pathway of pancreatic β-cells in driving mitochondrial dysfunction. The suppression of mitochondrial melatonin makes pancreatic β-cells susceptible to oxidative stress and dysfunctional mitophagy, partly mediated by the loss of melatonin’s induction of PTEN-induced kinase 1 (PINK1), thereby suppressing mitophagy and increasing autoimmune associated major histocompatibility complex (MHC)-1. The immediate precursor to melatonin, N-acetylserotonin (NAS), is a brain-derived neurotrophic factor (BDNF) mimic, via the activation of the BDNF receptor, TrkB. As both the full-length and truncated TrkB play powerful roles in pancreatic β-cell function and survival, NAS is another important aspect of the melatonergic pathway relevant to pancreatic β-cell destruction in T1DM. The incorporation of the mitochondrial melatonergic pathway in T1DM pathophysiology integrates wide bodies of previously disparate data on pancreatic intercellular processes. The suppression of Akkermansia muciniphila, Lactobacillus johnsonii, butyrate, and the shikimate pathway—including by bacteriophages—contributes to not only pancreatic β-cell apoptosis, but also to the bystander activation of CD8+ T cells, which increases their effector function and prevents their deselection in the thymus. The gut microbiome is therefore a significant determinant of the mitochondrial dysfunction driving pancreatic β-cell loss as well as ‘autoimmune’ effects derived from cytotoxic CD8+ T cells. This has significant future research and treatment implications.
Examining Regression Analysis Beyond the Mean of the Distribution using Quantile Regression: A Case Study of Modeling the Internal Bond of Medium Density Fiberboard using Multiple Linear Regression and Quantile Regression with an Example of Reliability Methods using R Software

I am submitting herewith a thesis written by Leslie Brooke Shaffer entitled “Examining Regression Analysis Beyond the Mean of the Distribution using Quantile Regression: A Case Study of Modeling the Internal Bond of Medium Density Fiberboard using Multiple Linear Regression and Quantile Regression with an Example of Reliability Methods using R Software. ” I have examined the final electronic copy of this thesis for form and content and recommend that it be accepted in partial fulfillment of requirements for the degree of Master of Science, with a major in Statistics.