Miracle-dz/UniFAR-FTUs huggingface dataset:
Determining the Population Doubling Time of HepG2 and Huh-7 Cells and the Toxic Effect of Dimethyl Sulfoxide (DMSO)

Developing cell lines that carry promising qualities closest to human hepatocytes in drug studies is a dream of many research laboratories. About 90% of drugs are metabolised by the liver. Therefore, most drug discovery studies utilise hepatocytes to understand the basic mechanism of the drug’s breakdown. To assure hepatocytes survival, various solvents were tested for their cytotoxicity on the cell line. Since most drugs are weakly soluble in water, they can be dissolved in an aprotic solvent. To obtain accurate results, the requirements of these solvents are biocompatible and non-toxic to the cells. One of the most commonly used solvents is dimethyl sulfoxide (DMSO). This study aims to understand the cell characteristic of cells doubling time and investigate the toxic effect of DMSO using the cell proliferation measurement technique. The toxicity was measured on liver-derived cell lines HepG2 and Huh-7. Cell growth and morphology were observed with an inverted phase microscope while cell viability was counted using Vi-Call XR 2.03. Results showed that a doubling time of 32 hours would be best for treatments on both cells. A concentration of more than 0.40% DMSO has significant toxicity and caused inhibition of proliferation on both cell lines, resulting in low cell count and higher cell death. Ethanol and methanol were observed as good solvents since they have low toxicity. However, drugs that were only dissolved in a low concentration of DMSO (0.05-0.2%) gave the best result without any harmful effect on cell proliferation.
Gravity darkening in rotating stars

Context. Interpretation of interferometric observations of rapidly rotating stars requires a good model of their surface effective temperature. Until now, laws of the form Teff ∝ g βff have been used, but they are only valid for slowly rotating stars. Aims. We propose a simple model that can describe the latitudinal variations in the flux of rotating stars at any rotation rate. Methods. This model assumes that the energy flux is a divergence-free vector that is antiparallel to the effective gravity. Results. When mass distribution can be described by a Roche model, the latitudinal variations in the effective temperature only depend on a single parameter, namely the ratio of the equatorial velocity to the Keplerian velocity. We validate this model by comparing its predictions to those of the most realistic two-dimensional models of rotating stars issued from the ESTER code. The agreement is very good, as it is with the observations of two rapidly rotating stars, α Aql and α Leo. Conclusions. We suggest that as long as a gray atmosphere can be accepted, the inversion of data on flux distribution coming from interferometric observations of rotating stars uses such a model, which has just one free parameter.
The Event Horizon Telescope: exploring strong gravity and accretion physics

The Event Horizon Telescope (EHT), a global sub-millimeter wavelength very long baseline interferometry array, is now resolving the innermost regions around the supermassive black holes Sgr A* and M87. Using black hole images from both simple geometric models and relativistic magnetohydrodynamical accretion ow simulations, we perform a variety of experiments to assess the promise of the EHT for studying strong gravity and accretion physics during the stages of its development. We nd that (1) the addition of the LMT and ALMA along with upgraded instrumentation in the \Complete" stage of the EHT allow detection of the photon ring, a signature of Kerr strong gravity, for predicted values of its total ux; (2) the inclusion of coherently averaged closure phases in our analysis dramatically improves the precision of even the current array, allowing (3) signicantly tighter constraints on plausible accretion models and (4) detections of structural variability at the levels predicted by the models. While observations at 345 GHz circumvent problems due to interstellar electron scattering in line-of-sight to the galactic center, short baselines provided by CARMA and/or the LMT could be required in order to constrain the overall shape of the accretion ow. Given the systematic uncertainties in the underlying models, using the full complement of two observing frequencies (230 and 345 GHz) and sources (Sgr A* and M87) may be critical for achieving transformative science with the EHT experiment.
Histomorphometric Analysis of Residual Alveolar Ridge Preserved using Collagen Cell Occlusive Membrane Alone and Along with Demineralized Bone Matrix Following Tooth Extraction: A Randomized Control Trial

Context: The changes in the volume and dimensions of the alveolar bone after tooth extraction often lead to challenges in prosthetic rehabilitation of the same necessitating ridge preservation procedures (RPP). Aim: The aim of this randomized clinical trial was to evaluate and compare the dimensional and histomorphometric changes of the sites preserved using the collagen membrane with and without demineralized bone matrix (DMBM). Settings and Design: Interventional, parallel-design, double blinded, randomized controlled trail. Materials and Methods: A randomized controlled trial was designed with 45 participants having at least 2 teeth indicated for were enrolled in this study. The sites were randomly assigned to the control group (RPP using collagen membrane) and the test group (RPP using collagen membrane with DMBM). The clinical parameters assessed were alveolar bone width and alveolar bone height. Histomorphometric analysis was carried out on tissue trephined from the preserved sites to evaluate the percentage of bone and connective tissue (CT %) formed 8 months postRPP. Statistical Analysis Used: Shapiro − Wilk test and paired and unpaired t-test. Results: Horizontal resorption was significantly less in the test group (7.375 ± 1.64). Histomorphometry of these sites revealed a complete absence of residual graft particles, presence of trabecular bone, and a more mineralized matrix (63.256%) as compared to the control sites (46.833%). Conclusions: The use of DMBM along with the collagen membrane for RPP yielded better results both clinically and histomorphometically.
HUBBLE SPACE TELESCOPE OBSERVATIONS OF AN EXTRAORDINARY FLARE IN THE M87 JET

HST-1, a knot along the M87 jet located 0. ″85 from the nucleus of the galaxy, has experienced dramatic and unexpected flaring activity since early 2000. We present analysis of Hubble Space Telescope near-ultraviolet (NUV) imaging of the M87 jet from 1999 May to 2006 December that reveals that the NUV intensity of HST-1 has increased 90 times over its quiescent level and outshines the core of the galaxy. The NUV light curve that we derive is synchronous with the light curves derived in other wavebands. The correlation of X-ray and NUV light curves during the HST-1 flare confirms the synchrotron origin of the X-ray emission in the M87 jet. The outburst observed in HST-1 is at odds with the common definition of active galactic nucleus variability usually linked to blazars and originating in close proximity to the central black hole. In fact, the M87 jet is not aligned with our line of sight and HST-1 is located at one million Schwarzchild radii from the supermassive black hole in the core of the galaxy.
Importance of transverse dipoles in the stability of biaxial nematic phase: A Monte Carlo study

Monte Carlo simulation performed on a lattice system of biaxial molecules possessing D 2h symmetry and interacting with a second rank anisotropic dispersion potential yields three distinct macroscopic phases depending on the biaxiality of the constituent molecules. The phase diagram of such a system as a function of molecular biaxiality is greatly modified when a transverse dipole is considered to be associated with each molecule so that the symmetry is reduced to C 2v . Our results indicate the splitting of the Landau point, i.e. the point in the phase diagram where a direct transition from the isotropic phase to the biaxial nematic phase occurs, into a Landau line for a system of biaxial molecules with strong transverse dipoles. The width of the Landau line becomes maximum for an optimal value of the relative dipolar strength. The presence of transverse dipoles leads to the stabilization of the thermotropic biaxial nematic phase at higher temperature and for a range of values of molecular biaxiality. The structural properties in the uniaxial and biaxial phases are investigated by evaluating the first rank and second rank orientational correlation functions. The dipole-induced long-range order of the anti-ferroelectric structure in the biaxial nematic phase, is revealed.
Insight into the Prospects for Tumor Therapy Based on Photodynamic Immunotherapy

Malignancy is one of the common diseases with high mortality worldwide and the most important obstacle to improving the overall life expectancy of the population in the 21st century. Currently, single or combined treatments, including surgery, chemotherapy, and radiotherapy, are still the mainstream regimens for tumor treatment, but they all present significant side effects on normal tissues and organs, such as organ hypofunction, energy metabolism disorders, and various concurrent diseases. Based on this, theranostic measures for the highly selective killing of tumor cells have always been a hot area in cancer-related fields, among which photodynamic therapy (PDT) is expected to be an ideal candidate for practical clinical application due to its precise targeting and excellent safety performance, so-called PDT refers to a therapeutic method mainly composed of photosensitizers (PSs), laser light, and reactive oxygen species (ROS). Photoimmunotherapy (PIT), a combination of PDT and immunotherapy, can induce systemic antitumor immune responses and inhibit continuing growth and distant metastasis of residual tumor cells, demonstrating a promising application prospect. This article reviews the types of immune responses that occur in the host after PDT treatment, including innate and adaptive immunity. To further help PIT-related drugs improve their pharmacokinetic properties and bioavailability, we highlight the potential improvement of photodynamic immunotherapy from three aspects: immunostimulatory agents, tumor-associated antigens (TAAs) as well as different immune cells. Finally, we focus on recent advances in various strategies and shed light on their corresponding mechanisms of immune activation and possible clinical applications such as cancer vaccines. Having discovered the inherent potential of PDT and the mechanisms that PDT triggers host immune responses, a variety of immunotherapeutic strategies have been investigated in parallel with approaches to improve PDT efficiency. However, it remains to be further elucidated under what conditions the immune effect induced by PDT can achieve tumor immunosuppression and to what extent PDT-induced antitumor immunity will lead to complete tumor rejection. Currently, PIT presents several outstanding intractable challenges, such as the aggregation ability of PSs locally in tumors, deep tissue penetration ability of laser light, immune escape, and biological toxicity, and it is hoped that these issues raised will help to point out the direction of preclinical research on PIT and accelerate its transition to clinical practice.
Student difficulties with quantum states while translating state vectors in Dirac notation to wave functions in position and momentum representations

We administered written free-response and multiple-choice questions and conducted individual interviews to investigate the difficulties that upper-level undergraduate and graduate students have with quantum states while translating state vectors in Dirac notation to wave functions in position and momentum representations. We find that students share common difficulties with translating a state vector written in Dirac notation to the wave function in position or momentum representation.
Gravitational field of one uniformly moving extended body and N arbitrarily moving pointlike bodies in post-Minkowskian approximation

High precision astrometry, space missions and certain tests of General Relativity, require the knowledge of the metric tensor of the solar system, or more generally, of a gravitational system of N extended bodies. Presently, the metric of arbitrarily shaped, rotating, oscillating and arbitrarily moving N bodies of finite extension is only known for the case of slowly moving bodies in the post-Newtonian approximation, while the post-Minkowskian metric for arbitrarily moving celestial objects is known only for pointlike bodies with mass-monopoles and spin-dipoles. As one more step towards the aim of a global metric for a system of N arbitrarily shaped and arbitrarily moving massive bodies in post-Minkowskian approximation, two central issues are on the scope of our investigation. (i) We first consider one extended body with full multipole structure in uniform motion in some suitably chosen global reference system. For this problem a co-moving inertial system of coordinates can be introduced where the metric, outside the body, admits an expansion in terms of Damour–Iyer moments. A Poincaré transformation then yields the corresponding metric tensor in the global system in post-Minkowskian approximation. (ii) It will be argued why the global metric, exact to post-Minkowskian order, can be obtained by means of an instantaneous Poincaré transformation for the case of pointlike mass-monopoles and spin-dipoles in arbitrary motion.
THE ECLIPSING BINARY CEPHEID OGLE-LMC-CEP-0227 IN THE LARGE MAGELLANIC CLOUD: PULSATION MODELING OF LIGHT AND RADIAL VELOCITY CURVES

We performed a new and accurate fit of light and radial velocity curves of the Large Magellanic Cloud (LMC) Cepheid—OGLE-LMC-CEP-0227—belonging to a detached double-lined eclipsing binary system. We computed several sets of nonlinear, convective models covering a broad range in stellar mass, effective temperature, and chemical composition. The comparison between theory and observations indicates that current theoretical framework accounts for luminosity—V and I band—and radial velocity variations over the entire pulsation cycle. Predicted pulsation mass—M = 4.14 ± 0.06 M☉—and mean effective temperature—Te = 6100 ± 50 K—do agree with observed estimates with an accuracy better than 1σ. The same outcome applies, on average, to the luminosity amplitudes and to the mean radius. We find that the best-fit solution requires a chemical composition that is more metal-poor than typical LMC Cepheids (Z = 0.004 versus 0.008) and slightly helium enhanced (Y = 0.27 versus 0.25), but the sensitivity to He abundance is quite limited. Finally, the best-fit model reddening—E(V − I) = 0.171 ± 0.015 mag—and the true distance modulus corrected for the barycenter of the LMC—μ0, LMC = 18.50 ± 0.02 ± 0.10 (syst) mag—agree quite well with similar estimates in the recent literature.
Socio-Economic Constraints of Adopting New Cowpea Varieties in Three Agro-Ecological Zones in the Senegalese Peanut Basin

Socio-economic constraints like gender, education, age, and income significantly affect the adoption of improved agricultural technologies. The objective of this study was to determine socio-economic factors that affect the adoption of improved cowpea varieties in the Senegalese peanut basin. The study was conducted in three (Bambey, Kebemer, and Kaffrine) of six regions of the peanut basin based on regional importance of cowpea production and rainfall gradient. In each study region, ten villages were selected, and random sampling was used to select eight heads of agricultural households within each village. The questionnaires were administered to 240 randomly selected farmers across the three study regions, 7 communes, and 30 villages. Results showed most heads of households were middle-aged (52–54 years old), married (95–100%), illiterate (84%), and men (95–100%). Households were mostly agriculture dependent (87%), low literacy rates (26% least primary school), and large family sizes (average of 15 members). The median cowpea yields across the study area varied from 35–100 kg ha−1, well below the ~300–400 kg ha−1 average yields reported for Senegal and Sub-Saharan Africa. The majority of farmers (67%) in the study regions did not use improved varieties, and the main reasons were low seed availability (78.8%) and limited access to technical knowledge and information (76.3%), but only 5.8% indicated seed price as a barrier to improved variety adoption. Major uses of cowpea in the study area were for marketing, livestock feed, and human consumption. In Kaffrine, fodder production was the major (85%) criterion for cowpea variety selection, whereas in the north (Bambey and Kebemer), taste, maturity date, and grain yield were major selection criteria. Factors that had positive effect on the likelihood of using improved cowpea varieties include; access to extension services, membership in farmers’ organization, cowpea being the main crop of production, organic farming, market, and livestock-oriented production systems, access to farmland and credit, dependence on agriculture as the main source of household income, and education of head of household. We conclude that there is a critical need for training, access to improved seeds, awareness, and financial support to producers to increase the adoption of new improved cowpea varieties, yields, profitability, and nutritional security among smallholder farmers in the Senegalese peanut basin.
SPECTROSCOPIC OBSERVATIONS OF PLANETARY NEBULAE IN THE NORTHERN SPUR OF M31

We present spectroscopy of three planetary nebulae (PNe) in the Northern Spur of the Andromeda galaxy (M31) obtained with the Double Spectrograph on the 5.1 m Hale Telescope at the Palomar Observatory. The samples were selected from the observations of Merrett et al. Our purpose is to investigate the formation of the substructures of M31 using PNe as a tracer of chemical abundances. The [O iii] λ4363 line is detected in the spectra of two objects, enabling temperature determinations. Ionic abundances are derived from the observed collisionally excited lines, and elemental abundances of nitrogen, oxygen, neon, sulfur, and argon are estimated. We study the correlations between oxygen and the α-element abundance ratios using our sample and the M31 disk and bulge PNe from the literature. In one of the three PNe, we observed a relatively higher oxygen abundance compared to the disk sample of M31 at similar galactocentric distances. The results of at least one of the three Northern Spur PNe might be in line with the proposed possible origin of the Northern Spur substructure of M31, i.e., the Northern Spur is connected to the Southern Stream and both substructures comprise the tidal debris of the satellite galaxies of M31.
The chromosphere above sunspots at millimeter wavelengths

Aims. The aim of this paper is to demonstrate that millimeter wave data can be used to distinguish between various atmospheric models of sunspots, whose temperature structure in the upper photosphere and chromosphere has been the source of some controversy. Methods. We use observations of the temperature contrast (relative to the quiet Sun) above a sunspot umbra at 3.5 mm obtained with the Berkeley-Illinois-Maryland Array (BIMA), complemented by submm observations from Lindsey & Kopp (1995 )a nd 2 cm observations with the Very Large Array. These are compared with the umbral contrast calculated from various atmospheric models of sunspots. Results. Current mm and submm observational data suggest that the brightness observed at these wavelengths is low compared to the most widely used sunspot models. These data impose strong constraints on the temperature and density stratifications of the sunspot umbral atmosphere, in particular on the location and depth of the temperature minimum and the location of the transition region. Conclusions. A successful model that is in agreement with millimeter umbral brightness should have an extended and deep temperature minimum (below 3000 K). Better spatial resolution as well as better wavelength coverage are needed for a more complete determination of the chromospheric temperature stratification above sunspot umbrae.
Efficient data structures for masks on 2D grids

This article discusses various methods of representing and manipulating arbitrary coverage information in two dimensions, with a focus on space- and time-e ciency when processing such coverages, storing them on disk, and transmitting them between computers. While these considerations were originally motivated by the specific tasks of representing sky coverage and cross-matching catalogues of astronomical surveys, they can be profitably applied in many other situations as well.
An overview of the completed Canada-France-Hawaii Telescope Lensing Survey (CFHTLenS)

The Canada-France-Hawaii Telescope Legacy Survey (CFHTLS) represents the most powerful weak lensing survey carried out to date. The CFHTLenS (Canada-France-Hawaii Telescope Lensing Survey) team was formed in 2008 to analyse the data from the CFHTLS focussing on a rigorous treatment of systematic effects in shape measurements and photometric redshifts. Here we review the technical challenges that we faced in analysing these data and their solutions which set the current standard for weak lensing analyses. We also present some science highlights that were made possible by this effort including cosmic shear tomography, tests for modified gravity models, and the mapping of dark matter structures over unprecedentedly large scales. An outlook is given on current and future surveys that are analysed with the tools prepared for CFHTLenS. CFHTLenS represents the first and only weak lensing data set that has been made publicly available so far. We encourage other surveys to follow this example.
Shifting and persisting in the face of life stressors: consequences for adolescent health

Abstract The current study assessed whether greater use of shift-and persist strategies, which entail the reappraisal of stressors (shifting) and endurance through optimism and meaning-making (persist), buffered the associations between life stressors and adolescents’ psychological health (i.e. depressive symptoms and anxiety) and physical health and health behaviors (i.e. self-rated health and sleep quality). Survey data were drawn from a racially/ethnically and socioeconomically diverse sample of 750 9th grade adolescents (53% female). Path analysis revealed racial discrimination, neighborhood risk, and deportation exposure were linked to poorer psychological and physical health, while socioeconomic disadvantage was related to less anxiety but poorer physical health. Some evidence suggested that shift-and-persist may be protective-reactive, wherein shift-and-persist typically promoted well-being across health domains but more so when the life stressors were at low versus high levels. Shift-and-persist strategies promote both mental and physical health, but the promotive effects appear to be maximized when adolescents’ exposure to life stressors is minimal.
Current Concept Review: Risk Factors for Infection Following Open Fractures

Abstract Infection following open fracture is a significant source of morbidity and mortality. Therefore, a central tenet of treatment is to minimize the risk of infection. The initial risk of infection is determined by wound characteristics, such as size, soft tissue coverage, vascular injury, and contamination. While no consensus exists on optimal antibiotic regimen, early administration of prophylactic antibiotics, within an hour of injury, when possible, has been shown definitively to decrease the risk of infection. Infection risk is further reduced by early irrigation with normal saline and aggressive debridement of devitalized tissue. Patient factors that increase risk of infection following open fracture include diabetes mellitus, smoking, male gender, and lower extremity fracture.