Miracle-dz/UniFAR-FTUs huggingface dataset:
Inflammation and lymphocyte infiltration are associated with shorter survival in patients with high-grade glioma

ABSTRACT Glioma represents a serious health burden in terms of morbidity and mortality. The prognostic significance of the lymphoid and myeloid infiltrates in glioma is not clearly determined. Moreover, the characterization of different leukocyte subsets in the tumor microenvironment relies mainly on immunohistochemistry observations, and data about their association with prognosis are contradictory. Here, we performed acomprehensive study of both the tumor-infiltrating and circulating immune compartments of patients with high-grade glioma. Nineteen tumor biopsies and 30 PBMC samples were analyzed by RNA sequencing. Validation was performed on The Cancer Genome Atlas (TCGA) RNA sequencing data from glioma and on additional 39 tumor biopsies analyzed by flow cytometry. We identified prognostic tumor and peripheral immune signatures, which associate increased inflammation, immune infiltration and activation with shorter overall survival in high-grade glioma patients. Importantly, we confirmed our observations by flow cytometry analysis and validated the tumor-signature using the TCGA dataset. In addition, both tumor genotype and grade associated with the degree of glioma immune infiltration. Unlike in the majority of cancers, lymphocyte infiltration at the tumor site is anegative prognostic factor in glioma, suggesting the ambivalent pro-tumorigenic role of immune responses in glioma.
Optimization of Sorghum Kafirin Extraction Conditions and Identification of Potential Bioactive Peptides

The interest in extracting kafirins (KAF), the main storage protein from sorghum grain has recently increased due to its gluten-free content and the significant scientific evidence showing the health benefits of the bioactive peptides from cereal grains in human diets. The objectives were to obtain the highest percentage of KAF extraction using amyloglucosidase as pretreatment to increase the extraction yield and predict the bioactive peptides in the KAF. In this study, pretreatments with amyloglucosidase increased the extraction yield of KAF compared with extraction methods using only ethanol and sodium metabisulfite. Two protein fragment sequences were identified from KAF extract and were evaluated for potential bioactive peptide using the BIOPEP-UWM database, which suggest that KAF proteins from white sorghum may be considered as good precursors of dipeptidyl peptidase-inhibitor, angiotensin-converting enzyme inhibitor, antioxidant and hypotensive peptides following chymotrypsin, thermolysin, and subtilisin and their combination. Average scores aligned using PeptideRanker confirmed KAF proteins' potential sources of bioactive peptides with over 5 peptides scored over 0.8. In addition, 31 unexplored peptide sequences that could have biological activity were identified. Our results suggest that KAF can be used in the peptide productions with potential biological activity and beyond.
Dysglycemia and Arthroplasty Outcomes: A Review

Arthroplasty (ART) is a common surgery and it is on the rise worldwide due to increasing longevity and osteoarthritis. The effects of perioperative hyperglycemia on the outcomes are largely unknown and the current review aimed to assess the impact of perioperative hyperglycemia on ART outcomes. The literature in PubMed and Google Scholar was searched for relevant articles published in the last ten years up to February 2020. The keywords knee ART, hip ART, diabetes mellitus (DM) impaired fasting glucose (IFG), impaired glucose tolerance (IGT), and impaired glycated hemoglobin (HbA1c)were used. Among the 113 articles retrieved, 34 full-texts were eligible, and only 21 studies (17 from the USA, three from Europe, and two from Asia) met the inclusion criteria for the systematic review. The authors' names, year of publication, country, type of study, number of patients, and duration of the study were reported. The studies reviewed showed high ART complication rates including infections, loosening, increasing severity and depth of infection, more pain, and higher costs with high perioperative hyperglycemia. The cut-off glycated hemoglobin values associated with complications ranged from 6.7 to >8.
Age-related mutational signature negatively associated with immune activity and survival outcome in triple-negative breast cancer

ABSTRACT Triple-negative breast cancer (TNBC) is characterized by broad genomic and transcriptional heterogeneity and results in a worse prognosis than other breast cancer types. Here, we integrated genomic and transcriptomic data combined with clinicopathologic information from 538 patients with TNBC and identified four novel significantly mutated genes (SMGs), namely, KDM6A, CD86, RBM47, and IFNGR1. A mutational signature (known as age-related signature 1) featured by enrichment of C > T mutations at NpCpG trinucleotides was associated with worse survival in TNBC (HR, 1.76 [95% CI, 1.07–2.90]; P = .025). We also analyzed gene transcriptomic profiles of TNBC samples and identified immune regulation-related gene pathways (e.g., antigen processing presentation and interferon signaling), and the cell cycle was significantly altered in samples with different signature 1 activity groups. The analysis further revealed that signature 1 was associated with decreased tumor immunogenicity and immunocyte infiltration in TNBC. This negative correlation was also observed in lung adenocarcinoma and prostate cancer samples. Furthermore, we found that patients with mutational signature 1 were markedly associated with decreased tumor mutation burden, even after controlling for age, grade, histological type, lymph node status, mutations in BRCA1/2 and ATR, and APOBEC and homologous recombination repair deficiency signatures (OR, 0.19 [95% CI, 0.11–0.32]; P < .001). Overall, this study identified previously unreported SMGs and re-annotated that age-related signature 1 was associated with a weakened immune microenvironment and predictive of poor survival in TNBC, offering opportunities to stratify patients into optimal treatment plans based on genomic subtyping.
Aberrant Expression of NCAPG is Associated with Prognosis and Progression of Gastric Cancer

Introduction Gastric cancer (GC), one of the most prevalent malignancies, is the third-leading cause of cancer-related deaths globally. The aim of this study is to investigate the involvement of non-structural maintenance of chromosomes condensin I complex subunit G (NCAPG) in the prognosis of GC. Methods Western blotting and immunostaining were employed to measure the NCAPG level in gastric tissues and cells. Kaplan–Meier analysis was applied to analyze the prognostic value of NCAPG in GC. RNA interference was applied to investigate the influence of the NCAPG silencing on GC cell growth and spread. Results NCAPG overexpression was associated with several clinicopathologic characteristics, including nodal status (P = 0.0378), distant metastasis (P = 0.0088), staging (P = 0.0230), vascular invasion (P = 0.0012), and disease-free survival (P = 0.004). Kaplan–Meier analysis revealed that NCAPG overexpression was positively correlated to poor GC patients disease-free and overall survival (P = 0.004 and P < 0.001, respectively). Univariate Cox regression analysis showed that the overexpression of NCAPG was a prognostic biomarker of GC (P = 0.005). In cultured GC cells, the knockdown of NCAPG suppressed cell proliferation, migration and invasion. Meanwhile, further studies revealed that the NCAPG silencing induces the G0/G1 cell cycle arrest and accordingly represses cell division. Finally, Western blotting showed that NCPAG knockdown dysregulated cell cycle- and epithelial–mesenchymal transition-related molecules. Conclusion Overall, the results reveal that NCAPG overexpression is a candidate prognostic biomarker and potential therapeutic target in GC.
Downregulation of Hsa_circ_0000735 Inhibits the Proliferation, Migration, Invasion, and Glycolysis in Non-small-cell Lung Cancer by Targeting miR-940/BMPER Axis

Background Lung cancer is the most commonly diagnosed cancer and the major cause of cancer-related deaths worldwide. The increasing studies have demonstrated that circular RNA (circRNA) was involved in the progression of various cancers, including non-small-cell lung cancer (NSCLC). This study was designed to assess the expression, roles and functional mechanisms of circ_0000735 in NSCLC. Materials and Methods The expression levels of circ_0000735, miR-940 and bone morphogenetic protein binding endothelial cell precursor-derived regulator (BMPER) were estimated by the real-time quantitative polymerase chain reaction (RT-qPCR). The biological behaviors of NSCLC cells such as proliferation, migration and invasion were analyzed by cell counting kit-8 (CCK-8), colony-forming assays and transwell assay, respectively. Furthermore, extracellular acid ratio and lactate production were tested to assess glycolysis levels of NSCLC cells. The interaction relationship among circ_0000735, BMPER and miR-940 was analyzed by bioinformatics database and dual-luciferase reporter assay. The protein expression level of BMPER was assessed by Western blot assay. Tumorigenesis assay was established to clarify the functional roles of circ_0000735 in vivo. Results Circ_0000735 was upregulated and significantly correlated with overall survival in patients with NSCLC. In addition, the loss-of-functional experiments revealed that knockdown of circ_0000735 repressed proliferation, migration, invasion and glycolysis of NSCLC cells and tumor growth in vivo, which was overturned by overexpression of BMPER. Similarly, overexpression of circ_0000735 enhanced proliferation, migration, invasion, and glycolysis of NSCLC cells. In addition, we also confirmed that overexpression of miR-940 impeded proliferation, migration, invasion, and glycolysis of NSCLC cells. Furthermore, overexpression of BMPER abolished si-circ_0000735 induced effects on NSCLC cells. Conclusion Circ_0000735 regulated proliferation, migration, invasion, and glycolysis in NSCLC cells by targeting miR-940/BMPER axis.
Intermittent Hypoxic Exposure Reduces Endothelial Dysfunction

Intermittent exposure to hypoxia (IHE) increases the production of reactive oxygen and nitrogen species as well as erythropoietin (EPO), which stimulates the adaptation to intense physical activity. However, several studies suggest a protective effect of moderate hypoxia in cardiovascular disease (CVD) events. The effects of intense physical activity with IHE on oxi-inflammatory mediators and their interaction with conventional CVD risk factors were investigated. Blood samples were collected from elite athletes (control n = 6, IHE n = 6) during a 6-day IHE cycle using hypoxicator GO2 altitude. IHE was held once a day, at least 2 hours after training. In serum, hydrogen peroxide (H2O2), nitric oxide (NO), 3-nitrotyrosine (3-Nitro), proinflammatory cytokines (IL-1β and TNFα), high sensitivity C-reactive protein (hsCRP), and heat shock protein 27 (HSP27) were determined by the commercial immunoenzyme (ELISA kits) or colorimetric methods. Serum erythropoietin (EPO) level was measured by ELISA kit every day of hypoxia. IHE was found to significantly increase H2O2, NO, and HSP27 but to decrease 3NT concentrations. The changes in 3NT and HSP27 following hypoxia proved to enhance NO bioavailability and endothelial function. In the present study, the oxi-inflammatory mediators IL-1β and hsCRP increased in IHE group but they did not exceed the reference values. The serum EPO level increased on the 3rd day of IHE, then decreased on 5th day of IHE, and correlated with NO/H2O2 ratio (rs = 0.640, P < 0.05). There were no changes in haematological markers contrary to lipoproteins such as low-density lipoprotein (LDL) and non-high-density lipoprotein (non-HDL) which showed a decreasing trend in response to hypoxic exposure. The study demonstrated that IHE combined with sports activity reduced a risk of endothelial dysfunction and atherogenesis in athletes even though the oxi-inflammatory processes were enhanced. Therefore, 6-day IHE seems to be a potential therapeutic and nonpharmacological method to reduce CVD risk, especially in elite athletes participating in strenuous training.
Neuroprotective and Neurodegenerative Aspects of Coffee and Its Active Ingredients in View of Scientific Literature

Coffee and its components have several neuroprotective properties that lower the risk of cognitive decline and other neurodegenerative diseases. This study reviews the mechanisms by which coffee and its respective compounds affect the brain and its pathologies. Many epidemiological studies in this literature review have shown coffee to reduce the risk of developing dementia, stroke, and Alzheimer's disease. It may also have a positive impact on the disease course of amyotrophic lateral sclerosis, Parkinson's disease, and depression. The optimal benefits achieved from coffee in these pathologies rely on higher daily doses. Most of its effects are attributed to caffeine by the antagonism of adenosine receptors in the central nervous system; however, other coffee constituents like chlorogenic acids have also shown much promise in therapeutic value. Existing research considers coffee to have great potential, but additional studies are still needed to clarify the mechanisms and actual causal relationships in certain neuropathologies.
The north american breeding bird survey.

The North American Breeding Bird Survey (BBS) occupies a critical role as the primary source of largescale, long-term population data for over 400 of North America’s breeding birds. Since its inception in 1966, the main emphasis of this multinational program has been to provide information on the distribution and trends of all bird species regularly encountered on these surveys. In addition to identifying at risk bird species and informing biologically sound avian conservation and management decisions, many peer reviewed articles appear in journals annually in which scientists use the publicly available dataset to evaluate diverse and contemporary topics such as climate change, disease tracking, and invasive species research. As this database grows so too does the potential for future research, which underscores the importance and value of this and similar long-term datasets.
An Experimental and Theoretical Investigation into the Formation of Ketene (H2CCO) and Ethynol (HCCOH) in Interstellar Analog Ices

The formation of isomers of C2H2O—ketene (H2CCO), ethynol (HCCOH), and oxirene (c-CHCHO)—was investigated in interstellar ice analogs composed of carbon monoxide and water. Using tunable photoionization time-of-flight mass spectrometry to selectively ionize the isomer of interest, ketene and ethynol were detected as reaction products, but oxirene remains elusive. These findings demonstrate that organic compounds that are precursors to complex organic molecules can form without an organic source of carbon. Furthermore, we report the first plausible detection of ethynol in astrophysically relevant ices. These investigations were supported by theoretical calculations describing reaction energies, pathways, ionization energies, and harmonic frequencies.
Activation of Bacteriophage Mu momTranscription by C Protein Does Not Require Specific Interaction with the Carboxyl-Terminal Region of the α or ς70Subunit of Escherichia coli RNA Polymerase

ABSTRACT Late in its growth cycle, transcription of the phage Mumom promoter (Pmom) is activated by the phage gene product, C, a site-specific DNA binding protein. In vitro transcription analyses showed that this activation does not require specific contacts between C and the carboxyl-terminal region of the α or ς70 subunit of Escherichia coliRNA polymerase. Unexpectedly, these results are in contrast to those known for another Mu-encoded transcriptional activator, Mor, which has a high degree of sequence identity with C and appears to interact with the carboxyl termini of both α and ς70.
Rewards of Kindness?

s International, 37, 7032. Curry, O. S. , Rowland, L. A. , Van Lissa, C. J., Zlotowitz, S. , McAlaney, J., & Whitehouse, H. (2018). Happy to help? A systematic review and meta-analysis of the effects of performing acts of kindness on the T hi s do cu m en t is co py ri gh te d by th e A m er ic an Ps yc ho lo gi ca l A ss oc ia tio n or on e of its al lie d pu bl is he rs . T hi s ar tic le is in te nd ed so le ly fo r th e pe rs on al us e of th e in di vi du al us er an d is no t to be di ss em in at ed br oa dl y. 27 PROSOCIALITY AND WELL-BEING
Secretion and Accumulation of Aβ by Brain Vascular Smooth Muscle Cells from AβPP‐Swedish Transgenic Mice

Alzheimer amyloid-β is deposited in the neuropil and in brain blood vessels in transgenic Tg2576 mice that overexpress human amyloid-β precursor protein (AβPP) containing the Swedish mutation (AβPP-Swe). Because the AβPP transgene in Tg2576 mice is placed behind the PrP promoter, all amyloid-β, including vascular amyloid, is considered to be of neuronal origin. We studied the expression of the transgenic AβPP in smooth muscle cells cultured from brain blood vessels from Tg2576 mice. We found that brain vascular smooth muscle cells overexpressed human AβPP-Swe approximately 4 times the physiological levels of mouse AβPP. The cultured cells secreted abundant Aβ1–40 and Aβ1–42 and formed intracellular Aβ-immunoreactive granules. The percentage of cells containing intracellular Aβ and the amount of intracellular Aβ were significantly higher in cultures obtained from 14-month-old than from 4-month-old mice, as tested on first or second passages. During cell senescence in culture, intracellular accumulation of Aβ and C-terminal fragments of AβPP increased in cells derived from both 4- and 14-month-old mice. Vascular muscle cells from Tg2576 mice appear to be a valuable model of the intracellular accumulation of Aβ. We suggest that vascular muscle cells may be involved in the production of cerebrovascular amyloid in Tg2576 mice.
The structure and properties of Napin-seed storage protein from rape (Brassica napus L.)

Purification to homogeneity of 2S albumin storage protein from rape seeds (napin) was achieved and its secondary structure and conformational stability were characterised by circular dichroism (CD), and high-sensitivity differential scanning calorimetry (HS-DSC). Deconvolution of the far-UV CD spectrum of napin revealed about 25% of α-helix and 38% of β-sheet structure at neutral and slightly acid pH. HS-DSC data show a single peak of protein denaturation with maximum at 101°C and 88°C at pH 6.0 and 3.0, respectively. This discloses very high stability of napin tertiary structure. The thermal denaturation of napin is irreversible duc to secondary processes such as hydrophobic aggregation of the unfolded protein. The deconvolution of the transition profile at pH 3.0 carried out with assumption that napin aggregation does not contribute to the transition parameters allows to distinguish two constituent transitions which may be attributed to two different domains in napin folding.
Mechanisms of axotomy-induced motoneuron apoptosis

Firstly, the endogenous expression of death promoting genes, Bax and Caspase-3 is higher and the expression of anti-apoptotic Bcl-2 is lower in neonatal motoneurons in comparison with adult motoneurons. The relative ratio of Bcl-2:Bax expression increases -10fold during postnatal development, which may contribute to the increased survival of adult motoneurons after axotomy. Secondly, activation of the cell death pathway requires mitochondrial involvement. The release of death-inducing factors from the mitochondria is required for the activation of caspases, the proteases which rapidly breakdown the cell. The Bax-mediated pathway and an i i i excitotoxic/calcium-mediated pathway converge at the mitochondria to induce the release of proapoptotic factors that activate caspases and breakdown the cell. Thirdly, application of trophic factors, B D N F or G D N F , rescues neonatal motoneurons from apoptosis by preventing loss of the survival-signaling pathway (maintaining high levels of Ak t phosphorylation) and preventing activation of the mitochondrial death pathway. This thesis provides support for the concept that neonatal motoneuron survival is dependent on target-derived trophic factors and provides insights into the injury-induced apoptotic pathway and the importance of trophic factor signaling as a repressor of cell death. T A B L E O F C O N T E N T S Abstract ii Table of
A review of the occurrence, toxicity, and biodegradation of condensed thiophenes found in petroleum

Condensed thiophenes comprise a significant portion of the organosulfur compounds in petroleum and in other products from fossil fuels. Dibenzothiophene (DBT) has served as a model compound in biodegradation studies for over two decades. However, until quite recently, few other organosulfur compounds were studied, and their fates in petroleum-contaminated environments are largely unknown. This paper presents a review of the types of organosulfur compounds found in petroleum and summarizes the scant literature on toxicity studies with condensed thiophenes. Reports on the biodegradation of benzothiophene, alkylbenzothiophenes, DBT, alkylDBTs, and naphthothiophenes are reviewed with a focus on the identification of metabolites detected in laboratory cultures. In addition, recent reports on quantitative studies with DBT and naphtho[2,1-b]thiophene indicate the existence of polar sulfur-containing metabolites that have escaped detection and identification. Key words: biodegradation, condensed thiophenes, diben.
A criança e a cultura lúdica

Toda uma escola de pensamento, retomando os grandes temas românticos inaugurados por Jean-Paul Richter e E. T. A. Hoffmann, vê no brincar o espaço da criação cultural por excelência. Deve-se a Winnicott a reativação de um pensamento segundo o qual o espaço lúdico vai permitir ao indivíduo criar e entreter uma relação aberta e positiva com a cultura: "Se brincar é essencial é porque é brincando que o paciente se mostra criativo". Brincar é visto como um mecanismo psicológico que garante ao sujeito manter uma certa distância em relação ao real, fiel, na concepção de Freud, que vê no brincar o modelo do princípio de prazer oposto ao princípio de realidade. Brincar torna-se o arquétipo de toda atividade cultural que, como a arte, não se limita a uma relação simples com o real.
Neurocognitive Deficit in Schizophrenia: A Quantitative Review of the Evidence

The neurocognitive literature on test performance in schizophrenia is reviewed quantitatively. The authors report 22 mean effect sizes from 204 studies to index schizophrenia versus control differences in global and selective verbal memory, nonverbal memory, bilateral and unilateral motor performance, visual and auditory attention, general intelligence, spatial ability, executive function, language, and interhemispheric tactile-transfer test performance. Moderate to large raw effect sizes (d > .60) were obtained for all 22 neurocognitive test variables, and none of the associated confidence intervals included zero. The results indicate that schizophrenia is characterized by a broadly based cognitive impairment, with varying degrees of deficit in all ability domains measured by standard clinical tests.