Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Screening of Extended Spectrum β -lactamases in Klebsiella pneumoniae isolated from Clinical Sources

Klebsiella pneumoniae is a major pathogen responsiple for nosocomial infections in world hospitals . One of the causes for the drug resistance in the Klebsiella pneumoniae is the production of ESBls enzymes.This research documented TEM , SHV , and CTX-M for the ﬁrst time among Klebsiella pneumoniae in Al-Anbar hospitals of Iraqi. All studied clinical isolates of K. pneumoniae (30) resistant- beta lactam from 100 isolates were collected from different clinicals sources such as (burned, wounds, sputum,and urine samples). The susceptibility to different antibiotics was tested by VITEK-2 system. The bla TEM , SHV , and CTX-M genes were detected by conventional PCR and the result showed 30/30 (100%) strains harbored these genes. This results showed the coexistence of these genes in one strains of K. pneumoniae, while indicated widespread ESBLs in Anbar, Iraq.
Supplementary Material: Cross-Modal 3D Shape Generation and Manipulation

The following describes the content in each section in the supplementary material. – § 1 describes additional implementation details. – § 2 presents ablation study of the proposed model. – § 3 illustrates more details of our baselines including encoder-decoder networks [4] and EditNeRF [8]. – § 4 details our experimental settings (e.g., datasets, evaluations). – § 5 provides detailed analysis on the limitations of the proposed model. – § 6 provides more color editing results with diverse color scribbles.
The resolvent cocycle in twisted cyclic cohomology and a local index formula for the Podle's sphere

We continue the investigation of twisted homology theories in the context of dimen- sion drop phenomena. This work unifies previous equivariant index calculations in twisted cyclic cohomology. We do this by proving the existence of the resolvent cocycle, a finitely summable analogue of the JLO cocycle, under weaker smoothness hypotheses and in the more general setting of 'modular' spectral triples. As an application we show that using our twisted resolvent cocycle, we can obtain a local index formula for the Podles sphere. The resulting twisted cyclic cocycle has non-vanishing Hochschild class which is in dimension 2.
Circadian acclimatization of performance, sleep, and 6-sulfatoxymelatonin using multiple phase shifting stimuli

Misalignment between the environment and one’s circadian system is a common phenomenon (e.g., jet lag) which can have myriad negative effects on physical and mental health, mental and physiological performance, and sleep. Absent any intervention, the circadian system adjusts only 0.5-1.0 h per day to a shifted light-dark and sleep-wake schedule. Bright light facilitates circadian adjustment, but in field studies, bright light is only modestly better than no stimulus. Evidence indicates that exercise and melatonin can be combined with bright light to elicit larger shifts but no study has combined all of these stimuli or administered them at the times that are known to elicit the largest effects on the circadian system. The aims of this study are to compare the effects of different treatments on circadian adjustment to simulated jet lag in a laboratory. Following 2 weeks of home recording, 36 adults will spend 6.5 consecutive days in the laboratory. Following an 8 h period of baseline sleep recording on the participant’s usual sleep schedule on Night 1 (e.g., 0000-0800 h), participants will undergo a 26 h circadian assessment protocol involving 2 h wake intervals in dim light and 1 h of sleep in darkness, repeated throughout the 26 h. During this protocol, all urine voidings will be collected; mood, sleepiness, psychomotor vigilance, and pain sensitivity will be assessed every 3 h, forehead temperature will be assessed every 90 min, and anaerobic performance (Wingate test) will be tested every 6 h. Following, the circadian assessment protocol, the participant’s sleep-wake and light dark schedule will be delayed by 8 h compared with baseline (e.g., 0800-1400 h), analogous to travelling 8 times zones westward. This shifted schedule will be maintained for 3 days. During the 3 days on the delayed schedule, participants will be randomized to one of 3 treatments: (1) Dim Red Light + Placebo Capsules, (2) Bright Light Alone, (3) Bright Light + Exercise + Melatonin. During the final 26 h, all conditions and measures of the baseline circadian protocol will be repeated. Acclimatization will be defined by shifts in circadian rhythms of aMT6s, psychomotor vigilance, Wingate Anaerobic performance, mood, and sleepiness, and less impairments in these measures during the shifted schedule compared with baseline. We posit that Bright Light Alone and Bright Light + Exercise + Melatonin will elicit greater shifts in circadian rhythms and less impairments in sleep, mood, performance, and sleepiness compared with Dim Red Light + Placebo Capsules. We also posit that Bright Light + Exercise + Melatonin will elicit greater shifts and less impairments than Bright Light Alone.
Mobility and freedom of movement: A novel out-of-hospital treatment for pediatric patients with terminal cardiac insufficiency and a ventricular assist device

Background Due to rapid medical and technological progress, more and more pediatric patients with terminal cardiac insufficiency are being implanted with a ventricular assist device as a bridge to transplant without legal approval for hospital discharge. EXCOR® Active is a recently developed mobile driving unit for the EXCOR® ventricular assist device (EXCOR® VAD) with a long-lasting battery life that can manage small blood pumps, offering improved mobility for pediatric patients. This study strives to elaborate the requirements necessary for a safe home healthcare environment (HHE) for pediatric patients on EXCOR® VAD powered by the EXCOR® Active driving unit. Materials and methods Patient- and device-related preconditions (medical, ethical, psychological, technical, structural, organizational) were analyzed with regard to feasibility and safety in three individual patient cases. Included were pediatric patients with terminal cardiac insufficiency in a stable medical condition receiving in-hospital treatment with a univentricular or biventricular EXCOR® VAD powered by EXCOR® Active. Analysis was single-center, data was obtained 05/2020–02/2022. Results A total of three patients on EXCOR® VAD were identified for HHE treatment with the EXCOR® Active driving unit. Switch was performed safely and increased mobility led to improved psychomotor development and improved quality of life. No complications directly related to HHE-treatment occurred. One patient recently underwent an orthotopic heart transplant, one patient remains in HHE, and one patient died due to a complication not related to the HHE. Ethical approval for off-label use was obtained and patients and parents were given the required technical training and psychological support. Caregivers and medical professionals involved in the patients’ care at home were briefed intensely. Remote consultations were implemented and interdisciplinary in-hospital checks reduced to a long-term 4-week-scheme. Conclusion While it is challenging to discharge pediatric patients being treated with a paracorporeal ventricular assist device (EXCOR® VAD) from hospital, it is feasible and can be managed safely with the novel driving unit EXCOR® Active. A HHE may help to improve patients’ psychomotor development, offer normalized social contacts and strengthen both patients’ and parents’ physical and mental resources. Legal approval and another study with a larger sample size are warranted.
Construction and validation of a prediction model of extrahepatic metastasis for hepatocellular carcinoma based on common clinically available data

Objective This study aimed to investigate the clinical characteristics and risk factors of patients with hepatocellular carcinoma (HCC) with extrahepatic metastases (EHM) and to establish an effective predictive nomogram. Methods Clinical and pathological data from 607 patients with hepatocellular carcinoma admitted to the Affiliated Hospital of Qinghai University between 1 January 2015 and 31 May 2018 were documented, as well as demographics, clinical pathological characteristics, and tumor-related parameters to clarify clinical risk factors for HCC EHM. These risks were selected to build an R-based clinical prediction model. The predictive accuracy and discriminating ability of the model were determined by the concordance index (C-index) and the calibration curve. The results were validated with a bootstrap resample and 151 patients from 1 June 2018 to 31 December 2019 at the same facility. Results In multivariate analysis, independent factors for EHM were neutrophils, prothrombin time, tumor number, and size, all of which were selected in the model. The C-index in the EHM prediction model was 0.672 and in the validation cohort was 0.694. In the training cohort and the validation cohort, the calibration curve for the probability of EHM showed good agreement between the nomogram prediction and the actual observation. Conclusion The extrahepatic metastasis prediction model of hepatocellular carcinoma constructed in this study has some evaluation capability.
Bias correction of ERA-Interim reanalysis temperature for the Qilian Mountains of China

Air temperature is the primary indicator of climate change. Reanalysis temperature products are important datasets for temperature estimates over high-elevation areas with few meteorological stations. However, they contain biases in observations, so a bias correction is required to enhance the accuracy of modeling predictions. In this study, we used the temperature lapse-rate method to correct ERA-Interim reanalysis-temperature data in the Qilian Mountains of China from 1979 to 2017. These temperature lapse rates were based on observations (ΓObs) and on model internal vertical lapse rates derived from different ERA-Interim pressure levels (ΓERA). The results showed that the temperature lapse rates in warm periods were larger than those in cold periods. Both the original and corrected ERA-Interim temperature can significantly capture the warming trend exhibited by observations. In general, the temperature lapse rate method was reliable for correcting ERA-interim reanalysis-temperature data. Although ΓObs performed best in bias correction, it depends heavily on the density of ground observation stations and is not appropriate for remote areas with a low data coverage. Correction methods based on ΓERA were shown to be reliable for bias correction, and will be especially applicable to mountainous areas with few observation stations. Our results contribute to the improvement of quality of data products and enhance the accuracy of modeling of climate change effects and risks to the environment and human health.
Effects of swift heavy ion irradiation and annealing on the microstructure and recrystallizationof SiC pre-implanted with Sr ions

Polycrystalline SiC wafers were implanted with 360 keV strontium (Sr) ions at room temperature (RT)to a fluence of 2 × 1016 cm−2. Some of the implanted samples were irradiated with xenon (Xe) ions of 167 MeV to a fluence of 3.4 × 1014 cm−2 and 8.4 × 1014 cm−2at RT. The as-implanted and implanted then irradiated samples were vacuum annealed (isochronally) at temperatures ranging from 1,100 to 1,400°C in steps of 100°C for 5 h. Annealing induced modification of the microstructure of the implanted and swift heavy ions (SHIs) irradiated SiC was studied by Raman spectroscopy, scanning electron microscopy (SEM) and backscattering spectrometry (RBS). Sr ions bombardment caused formation of an amorphous layer in SiC, while irradiation by Xe ions led to partial recrystallization of the amorphized layer. After annealing at 1,100°C, the samples with low Sr retained ratio showed full recrystallization, while the samples with high Sr retained ratio showed poor recrystallization. This suggests that the presence of Sr within the implanted region inhibited the recrystallization of SiC. Annealing of the as-implanted samples at temperatures from 1,100°C and 1,200°Cresulted in larger average crystal size compared to the SHIsirradiated samples annealed in the same temperature range. The difference in the average crystal sizes between the as-implanted and SHIs irradiated samples was due to the differences in the nucleation rate per amorphous area in the two samples. Ramanspectroscopy results showedthat the intensity of the LO mode of SiC increases with increasing crystal size. However, several factors such as pores and defects in SiC play a role in the decrease of the LO mode intensity of SiC (even if the average crystal size is large).
Lowering COVID-19 vaccine hesitancy among immigrants in Norway: Opinions and suggestions by immigrants

Even though COVID-19 vaccine has been proved effective, vaccine uptake and coverage has been and still is a great concern across different immigrant groups. Vaccine hesitancy remains a barrier to accept the vaccine among immigrants across the globe—including Norway—despite higher rates of hospitalizations and deaths. This study aimed to explore the opinions and suggestions of immigrants on how to lower the COVID-19 vaccine hesitancy among immigrants in Norway. Qualitative interviews were conducted with 88 persons with different immigrant background. Data was analyzed using framework analysis, utilizing “3Cs model of vaccine hesitancy” as a theoretical framework. The analysis yielded five main themes related to factors that may lower the vaccine hesitancy among immigrants in Norway: (1) Effective cultural communication, (2) Vaccine advocacy through community engagement, (3) Motivating factors, (4) Collaborative efforts via government and healthcare, and (5) Incentives for vaccination. This study enhanced our understanding of factors that according to immigrants themselves may lower the vaccine hesitancy. The insights obtained in this study can contribute to a better understanding of the current status of vaccine uptake among immigrants and can further give directions on how to improve vaccine uptake in these groups in Norway.
Processing pro-drop features in heritage Turkish

Previous studies have reported that null subject is not completely lost in heritage speakers, but there is an increase in the production and acceptance of overt subjects. Turkish is a pro-drop language and as a typical feature of pro-drop languages, it requires obligatory verb agreement marking for sentences with null subjects. However, Turkish subject-verb agreement marking is an example of optional agreement in which the 3rd person plural subject has optionality and can be used with singular verb forms under certain conditions. The current study investigates the reading times (RTs) of plural-marked and unmarked verbs in sentences with overt and null subjects during real time sentence processing in comparison to non-heritage speakers of Turkish via a self-paced reading experiment. Significant differences were observed between the heritage and non-heritage speakers of Turkish indicating both quantitative and qualitative real-time processing differences between the two groups. These differences suggest that Turkish heritage speakers need more time to integrate the information in real time processing.
The effects of dendritic cell-based vaccines in the tumor microenvironment: Impact on myeloid-derived suppressor cells

Dendritic cells (DCs) are a heterogenous population of professional antigen presenting cells whose main role is diminished in a variety of malignancies, including cancer, leading to ineffective immune responses. Those mechanisms are inhibited due to the immunosuppressive conditions found in the tumor microenvironment (TME), where myeloid-derived suppressor cells (MDSCs), a heterogeneous population of immature myeloid cells known to play a key role in tumor immunoevasion by inhibiting T-cell responses, are extremely accumulated. In addition, it has been demonstrated that MDSCs not only suppress DC functions, but also their maturation and development within the myeloid linage. Considering that an increased number of DCs as well as the improvement in their functions boost antitumor immunity, DC-based vaccines were developed two decades ago, and promising results have been obtained throughout these years. Therefore, the remodeling of the TME promoted by DC vaccination has also been explored. Here, we aim to review the effectiveness of different DCs-based vaccines in murine models and cancer patients, either alone or synergistically combined with other treatments, being especially focused on their effect on the MDSC population.
Comprehensive analyses of the microRNA–messenger RNA–transcription factor regulatory network in mouse and human renal fibrosis

Objective: The aim of this study was to construct a microRNA (miRNA)–messenger RNA (mRNA)–transcription factor (TF) regulatory network and explore underlying molecular mechanisms, effective biomarkers, and drugs in renal fibrosis (RF). Methods: A total of six datasets were downloaded from Gene Expression Omnibus. “Limma” and “DESeq2” packages in R software and GEO2R were applied to identify the differentially expressed miRNAs and mRNAs (DEmiRNAs and DEmRNAs, respectively). The determination and verification of DEmiRNAs and DEmRNAs were performed through the integrated analysis of datasets from five mouse 7 days of unilateral ureteral obstruction datasets and one human chronic kidney disease dataset and the Human Protein Atlas (http://www.proteinatlas.org). Target mRNAs of DEmiRNAs and TFs were predicted by prediction databases and the iRegulon plugin in Cytoscape, respectively. A protein–protein interaction network was constructed using STRING, Cytoscape v3.9. 1, and CytoNCA. Functional enrichment analysis was performed by DIANA-miRPath v3.0 and R package “clusterProfiler. ” A miRNA–mRNA–TF network was established using Cytoscape. Receiver operating characteristic (ROC) curve analysis was used to examine the diagnostic value of the key hub genes. Finally, the Comparative Toxicogenomics Database and Drug-Gene Interaction database were applied to identify potential drugs. Results: Here, 4 DEmiRNAs and 11 hub genes were determined and confirmed in five mouse datasets, of which Bckdha and Vegfa were further verified in one human dataset and HPA, respectively. Moreover, Bckdha and Vegfa were also predicted by miR-125a-3p and miR-199a-5p, respectively, in humans as in mice. The sequences of miR-125a-3p and miR-199a-5p in mice were identical to those in humans. A total of 6 TFs were predicted to regulate Bckdha and Vegfa across mice and humans; then, a miRNA–mRNA–TF regulatory network was built. Subsequently, ROC curve analysis showed that the area under the curve value of Vegfa was 0.825 (p = 0.002). Finally, enalapril was identified to target Vegfa for RF therapy. Conclusion: Pax2, Pax5, Sp1, Sp2, Sp3, and Sp4 together with Bckdha-dependent miR-125a-3p/Vegfa-dependent miR-199a-5p formed a co-regulatory network enabling Bckdha/Vegfa to be tightly controlled in the underlying pathogenesis of RF across mice and humans. Vegfa could act as a potential novel diagnostic marker and might be targeted by enalapril for RF therapy.
NETosis is critical in patients with severe community-acquired pneumonia

Pneumonia is the fourth leading cause of death globally, and the reason for the high mortality rate of patients with severe community-acquired pneumonia (SCAP) remains elusive. Corticosteroid treatment reduces mortality in adults with SCAP but can cause numerous adverse events. Therefore, novel therapeutic targets need to be explored and new adjunctive immune drugs are urgently required. We analyzed the transcriptome data of peripheral blood leukocytes from patients with SCAP and healthy controls from three perspectives: differentially expressed genes, predicted functions of differentially expressed long non-coding RNAs, and transcriptional read-through. We discovered that the NETosis pathway was top-ranked in patients with SCAP caused by diverse kinds of pathogens. This provides a potential therapeutic strategy for treating patients. Furthermore, we calculated the correlation between the expression of genes involved in NETosis and the ratio of arterial oxygen partial pressure to fractional inspired oxygen. We identified four novel potential therapeutic targets for NETosis in patients with SCAP, including H4C15, H3-5, DNASE1, and PRKCB. In addition, a higher occurrence of transcriptional read-through is associated with a worse outcome in patients with SCAP, which probably can explain the high mortality rate of patients with SCAP.
Barriers and enablers to accessing support services offered by staff wellbeing hubs: A qualitative study

Background International efforts have been made to develop appropriate interventions to support the mental health needs of healthcare professionals in response to COVID-19. However, fewer staff have accessed these than expected, despite experiencing elevated levels of mental distress since the onset of the pandemic. Consequently, we aimed to examine the barriers and enablers for healthcare professionals in accessing interventions offered by a Staff Mental Health and Wellbeing Hub. Methods Twenty-five semi-structured interviews were conducted with healthcare, social care and voluntary, community and social enterprise (VCSE) sector staff. Data were analysed using thematic analysis. Results Four key themes were identified: (1) Environment and Atmosphere in the Workplace; (2) The Impacts of COVID-19; (3) Confidentiality; and (4) Awareness and Communication of Resources. Organisational environments were perceived as an important enabler of accessing the hub services for mental health and wellbeing support. This included the importance of recognising and responding to the ongoing pressures of COVID-19- specific challenges. Ensuring and communicating aspects of confidentiality, and ensuring clear and consistent communication of the benefits of the Hub may encourage help-seeking for mental health challenges among healthcare professionals. Discussion Our findings highlight important considerations to increase uptake and engagement with services to support the mental health and wellbeing of healthcare professionals and associated staff and volunteers. Organisations aiming to increase employee uptake of these services should regularly circulate consistent and clear emails about what these services offer, provide training and information for managers so they can support staff to access these services and ensure access is confidential.