Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessing sleep behaviors in Latino children and adolescents: what is known, what are we missing, and how do we move forward?

Purpose of review Sleep health is a commonly overlooked component of pediatric cardiometabolic risk. Disparities in sleep duration and meeting of pediatric sleep guidelines have been well documented among at-risk populations in the United States, including Latinos. However, sleep research often fails to describe or account for contextual and cultural factors impacting the ability for Latino families to meet guidelines. The current review focuses on recent findings related to measurement of sleep duration, understanding of contextual factors that impact sleep hygiene, and interventions designed to increase sleep duration and quality among U.S. Latino families with infants, young children, and adolescents. Recent findings Ten studies focusing on sleep health in U.S. Latino children, using different study designs were identified. Overall, cross-sectional studies confirmed inadequate sleep among Latino children, intervention studies demonstrated promise of culturally-sensitive health behavior education for improving sleep in early childhood, and qualitative studies highlighted neighborhood and cultural factors that impact sleep quality. Implications for clinical practice and research Rather than new prevalence studies on adherence to sleep recommendations among Latino families, research focusing on adapting clinical guidelines to accommodate the realities of many Latino families (e.g., co-sleeping and bedsharing) will advance our knowledge. A shift towards objective measurement of the 24-h period as well as evaluating specific contextual barriers that make It challenging to meet sleep guidelines for Latino children is needed.
Interpretation of relative teleseismic P wave residuals

Relative teleseismic P wave residuals between Alaskan-Aleutian stations from deep focus (>500 km) Fiji-Tonga earthquakes are examined in detail. At large station separations the effects of hypocenter mislocation become important. When both sources and stations are widely separated, we observe effects produced by differences between travel time tables, by plate structure beneath the stations, by deep expression of the descending plate at the source, and/or by lateral variations in lower mantle structure. When either source or station separations are small, these effects are considerably reduced, so that relative residuals between stations can be resolved to within ±0.1 s. Average levels of relative residuals between Alaskan stations can be explained to first order by a plate structure beneath Alaska.
Subandean thrust and fold belt of northwestern Argentina: Geometry and timing of the Andean evolution

The Subandean ranges of northwestern Argentina are an active thin-skinned fold and thrust belt. The main detachment level in Silurian shales dips 2–3W, and all the major east-verging faults detach from it. Important intermediate detachment levels in the Devonian shales generate lift-off structures and the decoupling of the lower and upper structural levels. The Subandean thrust belt has a minimum shortening of about 60 km (36%) at about 2240 latitude. The deformation started at about 8.5–9 Ma with the uplift of the El Pescado range and the formation of an important back thrust at the Cinco Picachos range. Fault generation gets young to the east; the Pintascayo range uplift started at 7.6 Ma, while the Baja Orn range uplift began at about 6.9 Ma. These two ranges continued growing simultaneously until at least 4.7 Ma. At San Antonio range, fault movement began at approximately 4.4 Ma, and the Aguarage uplift started at about 2.7 Ma. An important stage dominated by out-of-sequence movements spans from about 4.5 Ma to present. For both proposed models of shortening, the Quaternary rates of shortening between 8 and 11 mm/yr coincide well with global positioning system results from the area. The hydrocarbon generation and migration is contemporaneous with the deformation, enhancing the possibilities of hydrocarbon entrapment in the area.
Analysis of predictive factors in children with high suspicion of pulmonary embolism

Since there is a limited number of predictive models designed for pediatric patients with pulmonary embolism (PE), healthcare providers have resorted to utilizing the Wells criteria and PE Rule‐out Criteria (PERC) that have been validated for use in adults. The objective of the study is to identify the predictive factors for PE and assess the effectiveness of the PERC, Wells, and pediatric‐specific PE (PPE) criteria.
Evaluating preschool linear growth velocities: an interim reference illustrated in Nepal

Abstract Objective: An annualised linear growth velocity (LGV) reference can identify groups of children at risk of growing poorly. As a single velocity reference for all preschool ages does not exist, we present an interim tool, derived from published, normative growth studies, for detecting growth faltering, illustrating its use in Nepali preschoolers. Design: The WHO Child Growth Velocity Standard was adapted to derive 12-month increments and conjoined to the Tanner-Whitehouse Height Velocity Reference data yielding contiguous preschool linear growth annualised velocities. Linear restricted cubic spline regressions were fit to generate sex-specific median and standard normal deviate velocities for ages 0 through 59 months. LGV Z-scores (LGVZ) were constructed, and growth faltering was defined as LGVZ < –2. Setting: Use of the reference was illustrated with data from Nepal’s Tarai region. Participants: Children contributing the existing growth references and a cohort of 4276 Nepali children assessed from 2013 to 2016. Results: Fitted, smoothed LGV reference curves displayed monotonically decreasing 12-month LGV, exemplified by male/female annual medians of 26·4/25·3, 12·1/12·7, 9·1/9·4, 7·7/7·8 and 7/7 cm/years, starting at 0, 12, 24, 36 and 48 months, respectively. Applying the referent, 31·1 %, 28·6 % and 29·3 % of Nepali children <6, 6–11 and 12–23 months of age, and ∼6 % of children 24–59 months, exhibited growth faltering. Under 24 months, faltering velocities were more prevalent in girls (34·4 %) than boys (25·3 %) (P < 0·05) but comparable (∼6 %) in older preschoolers. Conclusions: A LGV reference, concatenated from extant data, can identify preschool groups at-risk of growth faltering. Application and limitations are discussed.
ATR phosphorylates DHX9 at serine 321 to suppress R-loop accumulation upon genotoxic stress

Abstract Aberrant DNA/RNA hybrids (R-loops) formed during transcription and replication disturbances pose threats to genome stability. DHX9 is an RNA helicase involved in R-loop resolution, but how DHX9 is regulated in response to genotoxic stress remains unclear. Here we report that DHX9 is phosphorylated at S321 and S688, with S321 phosphorylation primarily induced by ATR after DNA damage. Phosphorylation of DHX9 at S321 promotes its interaction with γH2AX, BRCA1 and RPA, and is required for its association with R-loops under genotoxic stress. Inhibition of ATR or expression of the non-phosphorylatable DHX9S321A prevents DHX9 from interacting with RPA and R-loops, leading to the accumulation of stress-induced R-loops. Furthermore, depletion of RPA reduces the association between DHX9 and γH2AX, and in vitro binding analysis confirms a direct interaction between DHX9 and RPA. Notably, cells with the non-phosphorylatable DHX9S321A variant exhibit hypersensitivity to genotoxic stress, while those expressing the phosphomimetic DHX9S321D variant prevent R-loop accumulation and display resistance to DNA damage agents. In summary, we uncover a new mechanism by which ATR directly regulates DHX9 through phosphorylation to eliminate stress-induced R-loops.
If you’re happy and you know it: neural correlates of self-evaluated psychological health and well-being

Abstract Psychological health and well-being have important implications for individual and societal thriving. Research underscores the subjective nature of well-being, but how do individuals intuit this subjective sense of well-being in the moment? This pre-registered study addresses this question by examining the neural correlates of self-evaluated psychological health and their dynamic relationship with trial-level evaluations. Participants (N = 105) completed a self-evaluation task and made judgments about three facets of psychological health and positive functioning—self-oriented well-being, social well-being and ill-being. Consistent with pre-registered hypotheses, self-evaluation elicited activity in the default mode network, and there was strong spatial overlap among constructs. Trial-level analyses assessed whether and how activity in a priori regions of interest—perigenual anterior cingulate cortex (pgACC), ventromedial prefrontal cortex (vmPFC) and ventral striatum—were related to subjective evaluations. These regions explained additional variance in whether participants endorsed or rejected items but were differentially related to evaluations. Stronger activity in pgACC was associated with a higher probability of endorsement across constructs, whereas stronger activity in vmPFC was associated with a higher probability of endorsing ill-being items, but a lower probability of endorsing self-oriented and social well-being items. These results add nuance to neurocognitive accounts of self-evaluation and extend our understanding of the neurobiological basis of subjective psychological health and well-being.
MHC II–EGFP Knock‐in Mouse Model

The MHC II–EGFP knock‐in mouse model enables us to visualize and track MHC‐II‐expressing cells in vivo by expressing enhanced green fluorescent protein (EGFP) fused to the MHC class II molecule under the MHC II beta chain promoter. Using this model, we can easily identify MHC‐II‐expressing cells, including dendritic cells, B cells, macrophages, and ILC3s, which play a key role as antigen‐presenting cells (APCs) for CD4+ T cells. In addition, we can also precisely identify and analyze APC‐containing tissues and organs. Even after fixation, EGFP retains its fluorescence, so this model is suitable for immunofluorescence studies, facilitating an unbiased characterization of the histological context, especially with techniques such as light‐sheet fluorescence microscopy. Furthermore, the MHC II–EGFP knock‐in mouse model is valuable for studying the molecular mechanisms of MHC II gene regulation and expression by making it possible to correlate MHC II expression (MHC II–EGFP) with surface fraction through antibody detection, thereby shedding light on the intricate regulation of MHC II expression. Overall, this model is an essential asset for quantitative and systems immunological research, providing insights into immune cell dynamics and localization, with a tool for precise cell identification and with the ability to study MHC II gene regulation, thus furthering the understanding of immune responses and underlying mechanisms © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.
Seasonal Change of the Atmospheric Heat Budget over the Southern Ocean from ECMWF and ERBE Data

Abstract The seasonal variation of the zonally averaged atmospheric energy budget between 60° and 70°S was estimated. This region is predominantly within the seasonal sea ice zone of the Southern Ocean, including some parts of the Antarctic continent. In the Southern Ocean, seasonal sea ice extent exhibits large amplitudes and affects the surface heat exchange considerably. Seasonal variation of the energy budget and its relationship to the surface condition should be clarified as the basic variation. In spite of its importance, the data to estimate energy budgets are extremely sparse in this sea ice zone. Hence, the global objective analyses with forecasting models are mainly used as data for the present study. The surface energy flux is obtained as a remainder term in the energy budget of the total atmosphere, with the energy divergence and changes to the energy content of the atmospheric column derived from the European Centre for Medium-Range Weather Forecasts (ECMWF) objective analyses, and with the .
Fluctuations in Sustained Attention Explain Moment-to-Moment Shifts in Children’s Memory Formation

Why do children’s memories often differ from adults’ after the same experience? Whereas prior work has focused on children’s immature memory mechanisms to answer this question, here we focus on the costs of attentional lapses for learning. We track sustained attention and memory formation across time in 7- to 10-year-old children and adults (n = 120) to show that sustained attention causally shapes the fate of children’s individual memories. Moreover, children’s attention lapsed twice as frequently as adults’, and attention fluctuated with memory formation more closely in children than adults. In addition, although attentional lapses impaired memory for expected events in both children and adults, they impaired memory for unexpected events in children only. Our work reveals that sustained attention is an important cognitive factor that controls access to children’s long-term memory stores. Our work also raises the possibility that developmental differences in cognitive performance stem from developmental shifts in the ability to sustain attention.
Influence of gender and combined estrogen–progestin oral contraceptive on parotid saliva flow rate, pH, and electrolytes concentration

Abstract Objectives Endocrinal variations within an individual impact the electrolyte composition, pH, and flow‐rate (FR) of saliva. The aim of this study was to evaluate the gender‐specific differences and the effect of combined estrogen‐progestin oral contraceptives (COCs) on FR, pH, and electrolyte concentrations in the parotid saliva (PS) of a group of healthy adults. Material and Methods Stimulated PS was collected from 20 healthy adults using a Lashley cup; 11 males, 8 females, and 1 female undertaking combined contraceptive therapy (levonorgestrel/ethinyloestradiol 0.1 mg + 0.02 mg). FR and pH were recorded for each saliva sample. Electrolytes concentrations (Na+, Ca2+, K+, Mg2+) were measured using inductively coupled plasma optical emission spectrometer (ICP‐OES). Statistical analysis was performed, and the significance level was set at p < . 05. Results PS FR varied from 0.13 to 0.42 mL/min in females not taking any medication and from 0.08 to 0.5 mL/min in males not taking any medication. PS pH of females and males not taking any medication ranged from 6.23 to 7.50 and from 6.15 to 7.55. PS pH and FR of the female taking COCs were 6.5 and 0.1 mL/min. PS pH, FR, and electrolytes concentrations (Ca2+, Na+, K+, Mg2+) were not statistically significantly different between females and males not taking any medication. PS concentrations of Ca2+ and Na+ were significantly higher in the females taking COCs than in the females not taking any medication. Whereas, concentrations of K+ and Mg2+ did not differ significantly between the females taking COCs and the females not taking any medication. Conclusions There are no significant gender‐specific differences in PS flow rate, pH, and electrolyte concentrations of Na+, Ca2+, Mg2+, and K+. Combined hormonal oral contraceptive has a significant effect on PS flow rate, pH, Ca2+, and Na+ concentrations. Whereas the PS concentration of K+ and Mg2+ are not influenced by COCs. These results warrant further investigation.
Ultrasonic detection of vagus, accessory, and phrenic nerve atrophy in amyotrophic lateral sclerosis: Relation to impairment and mortality

In amyotrophic lateral sclerosis (ALS), phrenic nerve (PN) atrophy has been found, whereas there is controversy regarding vagus nerve (VN) atrophy. Here, we aimed to find out whether PN atrophy is related to respiratory function and 12‐month survival. Moreover, we investigated the relevance of VN and spinal accessory nerve (AN) atrophy in ALS.
Prognostic Value of Histopathological Thrombus Age in Large Vessel Occlusion-Related Stroke

Introduction: Acute mechanical thrombectomy (MT) is the preferred treatment for large vessel occlusion-related stroke. Histopathological research on the obtained occlusive embolic thrombus may provide information regarding the aetiology and pathology of the lesion to predict prognosis and propose possible future acute ischaemic stroke therapy. Methods: A total of 75 consecutive patients who presented to the Amphia Hospital with acute large vessel occlusion-related stroke and underwent MT were included in the study. The obtained thrombus materials were subjected to standard histopathological examination. Based on histological criteria, they were considered fresh (<1 day old) or old (>1 day old). Patients were followed for 2 years for documentation of all-cause mortality. Results: Thrombi were classified as fresh in 40 patients (53%) and as older in 35 patients (47%). Univariate Cox regression analysis showed that thrombus age, National Institutes of Health Stroke Scale at hospital admission, and patient age were associated with long-term mortality (p < 0.1). Multivariable Cox hazards and Kaplan-Meier analysis demonstrated that after extensive adjustment for clinical and procedural variables, thrombus age persisted in being independently associated with higher long-term mortality (hazard ratio: 3.34; p = 0.038, log-rank p = 0.013). Conclusion: In this study, older thromboemboli are responsible for almost half of acute large ischaemic strokes. Moreover, the presence of an old thrombus is an independent predictor of mortality in acute large vessel occlusion-related stroke. More research is warranted regarding future therapies based on thrombus composition.
The forkhead DNA-binding domain binds specific G2-rich RNA sequences

Abstract Transcription factors contain a DNA-binding domain ensuring specific recognition of DNA target sequences. The family of forkhead (FOX) transcription factors is composed of dozens of paralogs in mammals. The forkhead domain (FHD) is a segment of about 100 amino acids that binds an A-rich DNA sequence. Using DNA and RNA PCR-SELEX, we show that recombinant FOXL2 proteins, either wild-type or carrying the oncogenic variant C134W, recognize similar DNA-binding sites. This suggests that the oncogenic variant does not alter the intrinsic sequence-specificity of FOXL2. Most importantly, we show that FOXL2 binds G2-rich RNA sequences whereas it virtually fails to bind similar sequences in DNA chemistry. Interestingly, a statistically significant subset of genes responding to the knock-down of FOXL2/Foxl2 harbor such G2-rich sequences and are involved in crucial signaling pathways and cellular processes. In addition, we show that FOXA1, FOXO3a and chimeric FOXL2 proteins containing the FHD of the former are also able to interact with some of the preferred FOXL2-binding sequences. Our results point to an unexpected and novel characteristic of the forkhead domain, the biological relevance of which remains to be explored.
Natriuretic Peptide Receptor B Protects Against Atrial Fibrillation by Controlling Atrial cAMP Via Phosphodiesterase 2

BACKGROUND: β-AR (β-adrenergic receptor) stimulation regulates atrial electrophysiology and Ca2+ homeostasis via cAMP-dependent mechanisms; however, enhanced β-AR signaling can promote atrial fibrillation (AF). CNP (C-type natriuretic peptide) can also regulate atrial electrophysiology through the activation of NPR-B (natriuretic peptide receptor B) and cGMP-dependent signaling. Nevertheless, the role of NPR-B in regulating atrial electrophysiology, Ca2+ homeostasis, and atrial arrhythmogenesis is incompletely understood. METHODS: Studies were performed using atrial samples from human patients with AF or sinus rhythm and in wild-type and NPR-B-deficient (NPR-B+/−) mice. Studies were conducted in anesthetized mice by intracardiac electrophysiology, in isolated mouse atrial preparations using high-resolution optical mapping, in isolated mouse and human atrial myocytes using patch-clamping and Ca2+ imaging, and in mouse and human atrial tissues using molecular biology. RESULTS: Atrial NPR-B protein levels were reduced in patients with AF, and NPR-B+/− mice were more susceptible to AF. Atrial cGMP levels and PDE2 (phosphodiesterase 2) activity were reduced in NPR-B+/− mice leading to larger increases in atrial cAMP in the presence of the β-AR agonist isoproterenol. NPR-B+/− mice displayed larger increases in action potential duration and L-type Ca2+ current in the presence of isoproterenol. This resulted in the occurrence of spontaneous sarcoplasmic reticulum Ca2+ release events and delayed afterdepolarizations in NPR-B+/− atrial myocytes. Phosphorylation of the RyR2 (ryanodine receptor) and phospholamban was increased in NPR-B+/− atria in the presence of isoproterenol compared with the wildtypes. C-type natriuretic peptide inhibited isoproterenol-stimulated L-type Ca2+ current through PDE2 in mouse and human atrial myocytes. CONCLUSIONS: NPR-B protects against AF by preventing enhanced atrial responses to β-adrenergic receptor agonists.
Current and emerging biomarkers for ulcerative colitis

ABSTRACT Introduction Ulcerative colitis (UC) is a chronic illness requiring lifelong management that could be enhanced by personalizing care using biomarkers. Areas covered The main biomarker discovery modalities are reviewed, highlighting recent results across the spectrum of applications, including diagnostics (serum anti-αvβ6 antibodies achieving an area under the curve [AUC] = 0.99; serum oncostatin M AUC = 0.94), disease activity assessment (fecal calprotectin and serum trefoil factor 3: AUC > 0.90), prognostication of the need for treatment escalation (whole blood transcriptomic panels and CLEC5A/CDH2 ratio: AUC > 0.90), prediction of treatment response, and early identification of patients with subclinical disease. The use of established biomarkers is discussed, along with new evidence regarding autoantibodies, proteins, proteomic panels, transcriptomic signatures, deoxyribonucleic acid methylation patterns, and UC-specific glycomic and metabolic disturbances. Expert opinion Novel biomarkers will pave the way for optimized UC care. However, validation, simplification, and direct clinical translation of complex models may prove challenging. Currently, few candidates exist to assess key characteristics, such as UC susceptibility, histological disease activity, drug response, and long-term disease behavior. Further research will likely not only reveal new tools to tackle these issues but also contribute to understanding UC pathogenesis mechanisms.
Large‐scale margin collapse during Messinian early sea‐level drawdown: the SW Valencia trough, NW Mediterranean

In this study, detailed mapping of the ‘Messinian markers’ and examination of their geometrical relationships in the SW Valencia trough (Western Mediterranean) have revealed the style and depositional processes associated with emersion of continental margins during the Messinian Salinity Crisis (MSC). Based on multichannel seismic profiles and well data, this article evidences the existence of two Messinian depositional units in intermediate basins (Complex Unit and Upper Unit) and four main Messinian erosional surfaces (Margin Erosion Surface, Bottom Surface, Top (Erosion) Surface and Intermediate Surface). Results show that (1) initial rapid sea‐level drawdown and exposure of the shelf and upper slope of the Valencia margin induced large‐scale destabilization of the continental slope and deposition of large detrital bodies at the base‐of‐slope in the form of major mass‐transport deposits (MTD); (2) as sea level continued to drop, the development of the Margin Erosion Surface attained full development on the margins and eroded the clastic units (MTDs) deposited during initial drawdown. At the same time, a submarine drainage network formed in the deepwater Valencia trough; (3) persistent lowstand and restrictive conditions in the area resulted in deposition of the evaporites that form the Upper Unit in the SW Valencia trough.