Miracle-dz/UniFAR-FTUs huggingface dataset:
A Special Phenotype of Aconidial Aspergillus niger SH2 and Its Mechanism of Formation via CRISPRi

The complex morphological structure of Aspergillus niger influences its production of proteins, metabolites, etc., making the genetic manipulation and clonal purification of this species increasingly difficult, especially in aconidial Aspergillus niger. In this study, we found that N-acetyl-D-glucosamine (GlcNAc) could induce the formation of spore-like propagules in the aconidial Aspergillus niger SH2 strain. The spore-like propagules possessed life activities such as drug resistance, genetic transformation, and germination. Transcriptomic analysis indicated that the spore-like propagules were resting conidia entering dormancy and becoming more tolerant to environmental stresses. The Dac1 gene and the metabolic pathway of GlcNAc converted to glycolysis are related to the formation of the spore-like propagules, as evidenced by the CRISPRi system, qPCR, and semi-quantitative RT-PCR. Moreover, a method based on the CRISPR-Cas9 tool to rapidly recycle screening tags and recover genes was suitable for Aspergillus niger SH2. To sum up, this suggests that the spore-like propagules are resting conidia and the mechanism of their formation is the metabolic pathway of GlcNAc converted to glycolysis, particularly the Dac1 gene. This study can improve our understanding of the critical factors involved in mechanisms of phenotypic change and provides a good model for researching phenotypic change in filamentous fungi.
Prevalence of metabolic syndrome in beta-thalassemia major adult patients in Tanta University Hospitals

Introduction: one of long-term complications of Beta-thalassemia major (β-TM) disease is metabolic syndrome, which has a significant influence on patients' quality of life. Objective: to investigate the prevalence of metabolic syndrome in β-thalassemia major patients in Tanta University Hospitals. Materials and methods: our study included two groups. Group I: consist of (100) adult beta-thalassemia major patients. Group II: consist of (100) sex and age matched healthy subjects as control group. Results: the present study revealed that 29% of our β-thalassemia major patients had metabolic syndrome while in control group it was 2.0%, and low high-density lipoprotein (HDL)was the most frequent positive criterion it was 86.0% in β-thalassemia major patients' group. Prevalence of hyperglycemia, abdominal obesity, hypertriglyceridemia, and hypertension in patients with thalassemia was 29, 41, 21and 7%, respectively. Prevalence of metabolic syndrome in female patient was 34.3% while in male beta thalassemia major patient it was 18.2%. the prevalence of metabolic syndrome in well chelated beta thalassemia major patient was 0.0%, while in poorly chelated patients it was 35.4%. Conclusions: this study revealed that 29% of β-thalassemia major group patients had International Diabetes Federation (IDF) criteria of metabolic syndrome and low HDL was the most frequent positive criterion.
The role of partnership practices in strengthening parental trust

ABSTRACT Partnerships between parents and preschools (PPP), including parental trust as a key element, are crucial for child development. This exploratory study examined the type and intensity of partnership activities to which parents were invited and their contributions to parental trust, as well as preschool and teacher characteristics that predicted these activities. A total of 891 teachers and 728 families from 162 German preschools participated in the study. Path analyses showed parents reported higher levels of trust in preschools that offered a greater number of partnership activities for participation and decision-making as well as for parental education. In addition, results indicate that preschool and teacher characteristics, such as ethnic composition and professional competencies, predicted the type and intensity of partnership activities. Consequently, training programmes should make use of these results to enhance preschool teachers’ competencies with regard to PPP, and thereby to strengthen parental trust.
Using Multi-Task Learning-Based Framework to Detect ST-Segment and J-Point Deviation From Holter

Artificial intelligence is increasingly being used on the clinical electrocardiogram workflows. Few electrocardiograms based on artificial intelligence algorithms have focused on detecting myocardial ischemia using long-term electrocardiogram data. A main reason for this is that interference signals generated from daily activities while wearing the Holter monitor lowered the ability of artificial intelligence to detect myocardial ischemia. In this study, an automatic system combining denoising and segmentation modules was developed to detect the deviation of the ST-segment and J point. We proposed a ECG Bidirectional Transformer network that applied in both denoising and segmentation tasks. The denoising model achieved RMSEde, SNRimp, and PRD values of 0.074, 10.006, and 16.327, respectively. The segmentation model achieved precision, sensitivity (recall), and F1-score of 96.00, 93.06, and 94.51%, respectively. The system’s ability to distinguish the depression and elevation of the ST-segment and J point was also verified by cardiologists as well. From our ECG dataset, 103 patients with ST-segment depression and 10 patients with ST-segment elevation were detected with positive predictive values of 80.6 and 60% respectively. Using Holter ECG and transformer-based deep neural networks, we can detect subtle ST-segment changes in noisy ECG signals. This system has the potential to improve the efficacy of daily medicine and to provide a broader population-level screening for asymptomatic myocardial ischemia.
STEAM (SCIENCE, TECHNOLOGY, ENGINEERING, ART, AND MATHEMATICS) EDUCATION AND TEACHERS’ PEDAGOGICAL DISCONTENTMENT LEVELS

Literature focused on the emerging implementation of STEAM (Science, Technology, Engineering, Art, and Mathematics) education within K-12 education programs in the USA emphasizes the need for researchers to address the potential for disrupting the pedagogical contentment of teachers assigned to teach in STEAM disciplines who have no backgrounds in the fields represented by STEAM and/or who have no prior teaching experience in the areas of STEAM. Research objectives for the current study focused on examining the influence of intensive professional development on K-12 teachers’ pedagogical discontentment levels relative
Impact of growth hormones on vegetative propagation of Elaeocarpus ganitrus Roxb.

Elaeocarpus ganitrus Roxb. commonly identified as ‘rudraksha’, belonging to Elaeocarpaceae possess a great religious, spiritual and materialistic significance which is placed under the threatened category in the tropical wet evergreen forests of North East India. Because of its over-exploitation, poor germination, low viability rate and hard seed coat, an attempt has been done for mass production of planting materials through cuttings and air layering’s. Cuttings trials were conducted during three different periods, mid - March, mid – July and mid – November by using Indol butyric acid and Napthalene acetic acid of 1000, 2000, 4000, 6000 ppm where air layering’s were done during mid - May by treating IBA and NAA solution of 500, 1000 and 2000 ppm in combination with para-hydroxybenzoic acid of 2000 ppm as synergist. Application of IBA and NAA of different concentration did not encourage the growth in the cuttings in all three periods. Air layering’s treated with NAA 1000 ppm+2000ppm para- hydroxy benzoic acid and IBA 500 ppm +2000ppm para- hydroxy benzoic acid resulted the highest and same value of callusing and rooting percent (70 and 66.67%), respectively while minimum callusing and rooting percentage (56.67 and 51.67%) was recorded in control. The present study focused on the mass production of planting materials in shorter duration with a reasonable cost for enhancing production as well as socio-economic conditions.
Des)controle de parâmetros clínicos e antropométricos em indivíduos com doença arterial coronária

This cross-sectional study evaluated the clinical and anthropometrical parameters of 100 individuals with coronary artery disease, who were assisted at preventive cardiology outpatient clinic at a public hospital in Salvador/BA. Data collection was performed through interviews and both clinical and laboratory evaluations. The results were analyzed as averages and percentages. Most participants were black men, aged 200 mg/dl, 36% was overweight, 28% were obese, 65% were in soma stage of hypertension, 65% had low HDL-C and 43% had high total cholesterol. It was observed that individuals with high cardiovascular risk and deficient socioeconomic conditions show a lack of control of several cardiovascular risk factors, thus demanding effective health care practices to control the illness.
Reproducibility and Accuracy of the Radiofrequency Echographic Multi-Spectrometry for Femoral Mineral Density Estimation and Discriminative Power of the Femoral Fragility Score in Patients with Primary and Disuse-Related Osteoporosis

We aimed to investigate the reproducibility and accuracy of Radiofrequency Echographic Multi-Spectrometry (REMS) for femoral BMD estimation and the reproducibility and discriminative power of the REMS-derived femoral fragility score. 175 patients with primary and disuse-related osteoporosis were recruited: one femoral Dual-energy X-ray Absorptiometry (DXA) scan and two femoral REMS scans were acquired. No significant test—retest differences were observed for all REMS-derived variables. The diagnostic concordance between DXA and REMS was 63% (Cohen’s kappa = 0.31) in patients with primary osteoporosis and 13% (Cohen’s kappa: −0.04) in patients with disuse-related osteoporosis. No significant difference was observed between REMS and DXA for either femoral neck BMD (mean difference between REMS and DXA: −0.015 g/cm2) or total femur BMD (mean difference: −0.004 g/cm2) in patients with primary osteoporosis. Significant differences between the two techniques were observed in patients with disuse-related osteoporosis (femoral neck BMD difference: 0.136 g/cm2; total femur BMD difference: 0.236 g/cm2). Statistically significant differences in the fragility score were obtained between the fractured and non-fractured patients for both populations. In conclusion, REMS showed excellent test-retest reproducibility, but the diagnostic concordance between DXA and REMS was between minimal and poor. Further studies are required to improve the REMS—derived estimation of femoral BMD.
Role of Education in Poverty Mitigation in Pakistan: Evidence from Southern Punjab

Poverty is one of the most common and challenging issues in the world, especially for developing economies. The development pace will never be speedier until this issue is resolved beforehand. In the case of Pakistan, poverty is still persistent and increasing due to multifactor. The current study endeavors to investigate the multi-dimensional relation between educational attainment level and its impact on household poverty in South Punjab, Pakistan. For this purpose 800 households are selected as a sample. Ordinary Least Square and Binary Logistic methods are used. It transpired from the outcomes that variables that have a positive impact on poverty include the size of the household and family system. On the other hand, age of the head of household, education of the head of household, natural log of assets of the household, employment status of the household head, spouse participation in income generation, area of residence of the household, foreign remittances and unemployment status are adversely affecting. This study is endowed with regional and divisional level comparisons. It emerged from the result that amongst the three, Dera Ghazi Khan (DG Khan) is the most vulnerable. Family size, family system, and employment status have adversely affected the economic face of this division. It is recommended that an effective family planning regime, along with improved education attainment level and urbanization of the rural areas of this vicinity be strongly focused on a priority basis.
Prognostic Nutritional Index in Hepatocellular Carcinoma Patients With Hepatitis B Following US-Guided Percutaneous Microwave Ablation: A Retrospective Study With 1,047 Patients

Objective Several studies have revealed that the prognostic nutritional index (PNI) was associated with survival in several cancers. However, the prognostic value of PNI in hepatocellular carcinoma (HCC) patients following ultrasound-guided percutaneous microwave ablation (US-PMWA) remains unknown, especially in patients with hepatitis B virus (HBV) infection. Therefore, the present study aimed to evaluate the potential prognostic value of PNI in these patients. Materials The medical records of 1,047 HCC patients with HBV infection following US-PMWA were retrospectively reviewed. The association between preoperative PNI and overall survival (OS), as well as other clinical characteristics of HCC, were analyzed using the Kaplan–Meier plot, log-rank test, multi-parameter Cox proportional hazards model, restricted cubic spline (RCS), and time-dependent receiver operating characteristic (ROC) curve analyses. Results Patients with a preoperative PNI more than 45 were verified to have better OS than patients with a PNI less than 45. In the multi-parameter Cox proportional hazards models, the log-transformed PNI was verified as an independent prognostic factor for OS. The result of the RCS analysis revealed that there was a nearly linear relationship between PNI and OS. The area under the time-dependent ROC curve for PNI in predicting OS was 0.56, which is relatively stable. Conclusion Preoperative PNI represents a convenient, noninvasive, and independent prognostic indicator in HCC patients with HBV infection following US-PMWA.
Dental manifestations in adult hypophosphatasia and their correlation with biomarkers

Hypophosphatasia (HPP) is a genetic condition with broad clinical manifestations caused by alkaline phosphatase (ALP) deficiency. Adults with HPP exhibit a wide spectrum of signs and symptoms. Dental manifestations including premature tooth loss are common. Much of the published literature reporting dental manifestations consists of case reports and series of symptomatic patients, likely biased towards more severe dental manifestations. The objective of this study was to systematically explore the dental manifestations among adults with HPP by conducting a comprehensive dental evaluation. To minimize bias, the study explored dental manifestations in an unselected cohort of adults with HPP. Participants were identified searching electronic health record (EHR) data from a rural health system to discover adults with persistent ALP deficiency. Heterozygotes with pathogenic (P), likely pathogenic (LP), or uncertain variants (VUS) in ALPL and at least one elevated ALP substrate were defined as adults with HPP and underwent genetic, dental, oral radiographic, and biomarker evaluation. Twenty‐seven participants completed the study. Premature tooth loss was present in 63% (17/27); 19% (5/27) were missing eight or more teeth. Statistically significant associations were found between premature permanent tooth loss and HPP biomarkers ALP (p = 0.049) and bone‐specific ALP (p = 0.006). Serum ALP (ρ = −0.43, p = 0.037) and bone‐specific ALP (ρ = −0.57, p = 0.004) were negatively correlated with number of teeth lost prematurely. As noted with tooth loss, periodontal breakdown was associated with bone‐specific ALP. An inverse association between periodontal breakdown and bone‐specific ALP was observed (p = 0.014). These findings suggest a role for ALP in maintenance of dentition.
Sales promotion tools, customer emotions and consumer buying responses in Ghana

Sales promotion is used by various organizations to encourage buying response among their customers. Although it is an effective tool in marketing, few studies have been conducted on sales promotion on buying response using customer emotions as a mediating factor. To fill these gap, the study pursued the formation of a model that explains how sales promotion links to customers' emotions and buying responses in Ghana. The model was validated by conducting a survey with a convenience sampling technique on 664 customers. The data collected were processed using a structural equation modeling approach. The result shows that a bonus pack, coupons, sample, price discount, and rebate have a relation with customer buying response. All the tools have an indirect relationship with the customer buying response except for the bonus pack. Besides, there is a relationship between sales promotion tools and customer emotion, and customer emotions have a strong relationship with customer buying response. The study suggested that managers must create new value for their product to build an emotional connection with the product.
Recommendations for Multimodality Cardiovascular Imaging of Patients with Hypertrophic Cardiomyopathy Endorsed by the American Society of Nuclear Cardiology , Society for Cardiovascular Magnetic Resonance , and Society of Cardiovascular Computed Tomography

Sherif F. Nagueh, MD, FASE, Chair,* S. Michelle Bierig, RDCS, FASE,* Matthew J. Budoff, MD, Milind Desai, MD,* Vasken Dilsizian, MD, Benjamin Eidem, MD, FASE,* Steven A. Goldstein, MD,* Judy Hung, MD, FASE,* Martin S. Maron, MD, Steve R. Ommen, MD,* and Anna Woo, MD,*Houston, Texas; St. Louis, Missouri; Los Angeles, California; Cleveland, Ohio; Baltimore, Maryland; Rochester, Minnesota; Washington, District of Columbia; Boston, Massachusetts; Toronto, Ontario, Canada
Morphometric descriptive report of scleral ossicle rings, by ultrasound and computed tomography, in three Testudines specimens

ABSTRACT: The scleral ossicle rings function has been related to mechanical protection, muscle fixation, support for eyeball shape and visual accommodation. There are few morphobiometric reports on these rings in different Testudines species, and we performed ultrasound (US) and computed tomography (CT) of the scleral ossicle rings in one green turtle (Chelonia mydas), one black-bellied slider (Trachemys dorbigni) and one red-footed tortoise (Chelonoidis carbonarius). The US and CT of the ossicle rings were performed for anatomical identification. The thickness, density, width, and diameters of each ring were measured. The US and CT of the scleral ossicle rings of three animals showed single and continuous circular structures, located in the anterior pole. These structures were easily observed in C. mydas, whose rings were the biggest, thickest and widest. The T. dorbigni CT presented decreased dimensions and the ossicles were the most difficult to identify. Bone density in the superior region was greater than in the inferior of each ring in all animals. Non-invasive imaging exams are good tools to study the anatomy of the ocular skeleton. The scleral ossicle rings of the three specimens presented general morphological similarities and CT enabled visualizing a greater number of details of the ring bone morphology.
Relationship between socio-demographic characteristics and the employees’ knowledge about occupational hazards in Al Nasiriya Heart Center, Iraq: 2021-2022

Background: Occupational hazards are defined as workplace issues that have likely to raise the hazard of our health, which can be categorized as biological and non-biological. Health care providers are challenged with a variety these hazards including physical, chemical, and psychological ones. Material and method: a cross-sectional study was conducted among participants in Al Nasiriya Heart center done by face-to-face interviews to find out the relation between socio-demographic characteristics and occupational hazards. A total sample 300 employee were included in the study. Result: The study found a significant relationship between age group, Radiological and psychological risks of occupational hazards (p.value of 0.004 and 0.030). In regards to the relationship between educational level and risk factors of occupational hazard. The study also found a significant relationship between the level of education and biological risk of occupational hazards (P.value 0.0010) with non-significant relation between occupational hazard and gender. Conclusions: Occupational hazards have emerged as a major concern among health-care providers in Al Nasiriya Heart Center, as they are exposed to a wide range of risks, including biological, chemical, physical, and psychosocial risks.
Managing the delivery of venous leg ulcer services: A willingness to pay study

There is widespread variation in venous leg ulcer (VLU) wound care contributing to inadequate service provision resulting in poor outcomes to patients. Little has been published on the perspectives of where treatments should be carried out. The aim of the study was to quantify respondents' preferences for the preferred place of treatment for VLU.
The Community Role in the Implementation of Coronavirus Disease-19 Control Policies in Indonesia

Background: The success of COVID-19 control in Indonesia comes to place with active community participation. Community's role in supporting the successful implementation of COVID-19 policy is critical to investigate. This study, therefore, aimed to determine community’s role in supporting the successful implementation of COVID-19 control policies.Method: This study is exploratory research with a cross-sectional approach. Seven Indonesian regencies as research locations were selected purposively. Quantitative data were collected from 2,010 positive-COVID-19 patients and their close contacts through a standardized self-administered questionnaire. In-depth interviews were employed with 79 health center officers. Content analysis was used to analyze the qualitative data, and descriptive analysis was for the quantitative data. Results: This study had a relatively balanced gender distribution. There were 26.9% at age of 6-19 years, and 80.9% were in close contact with COVID-19 patients. Most respondents were from primary health care centers, had a bachelor's degree, and had worked for less than five years. Their adherence to health protocols was lower than the group that was not exposed to COVID-19. Most respondents followed health protocols such as hygiene (92.9 %) and cough etiquette (91.5 %). However, COVID-19 quarantine compliance was still low (38.2%), and 90.8% of respondents mostly practiced self-isolation at home. COVID-19 control strategies in each region quite varied. A lack of public awareness and education, flexibility in policy implementation, and a poor reward and punishment system were barriers to COVID-19 control. The community's role in supporting COVID-19 control policies includes helping to communicate COVID-19 risks, assisting healthcare centers in monitoring COVID-19 self-isolation, providing and operating integrated isolation shelters for confirmed cases, and participating as a case tracer.