Miracle-dz/UniFAR-FTUs huggingface dataset:
Modulatory effect of myricitrin against chromosome instability and cytostasis induced by bleomycin and oxaliplatin in CHO-K1 cells

Abstract Myricitrin (MYR), a flavonol consumed in the leaves and fruits of plants of the Myrtaceae family, presents anti-proliferative, anti-inflammatory, anti-diabetic, and antioxidant properties in humans. However, there are few studies regarding the cyto-genotoxicity and the chemopreventive potential of MYR. Using the in vitro Micronucleus test, the cytostasis, mutagenicity, and modulatory effect of MYR in CHO-K1 cells were assessed. The concentrations of 39 and 78 µg/mL (p < 0.001.) of MYR decrease the cytokinesis-block proliferation index (CBPI) in the short exposure treatment (4 h), while in the extended treatment (24 h), concentrations of 4.8, 9.7, 19.5, 39 and 78 µg/mL (p < 0.001.) decreased the CBPI. MYR associated with oxaliplatin decreased CBPI at all tested concentrations in the pre-(p < 0.001) and post-treatments (p < 0.001), but there was no decrease when associated with bleomycin. As for chromosome instability, MYR did not increase the frequency of micronuclei (MNi), nucleoplasmic bridges (NPBs), or nuclear buds (NBUDs) in the 4 h exposure time, however, in the 24 h treatment, MYR increased the frequency of MNi and NPBs at concentration 19.5 µg/mL (p < 0.001). As for the modulatory effect, MYR associated with bleomycin decreased the frequency of MNi, NPBs, and NBUDs at all concentrations in the pretreatment (MNi and NPBs p < 0.001, NBUDs p < 0.05) and simultaneously (MNi, NPBs and NBUDs p < 0.001). When associated with oxaliplatin, the simultaneous treatment decreased the frequency of MNi (p < 0.001) and NBUDs (p < 0.01) at all concentrations, however, in the post-treatment, MYR increased MNi (p < 0.001) and NPBs p < 0.05) in CHO-K1 cells, when compared to oxaliplatin alone. The results demonstrated that MYR could modulate the mutagenic and cytostatic actions of bleomycin and oxaliplatin, demonstrating distinct behaviors, depending on the mechanism of action of the chemotherapeutic agent. Graphical Abstract
Flexible Buprenorphine/Naloxone Model of Care for Reducing Opioid Use in Individuals With Prescription-Type Opioid Use Disorder: An Open-Label, Pragmatic, Noninferiority Randomized Controlled Trial.

OBJECTIVE Extensive exposure to prescription-type opioids has resulted in major harm worldwide, calling for better-adapted approaches to opioid agonist therapy. The authors aimed to determine whether flexible take-home buprenorphine/naloxone is as effective as supervised methadone in reducing opioid use in prescription-type opioid consumers with opioid use disorder. METHODS This seven-site, pan-Canadian, 24-week, pragmatic, open-label, noninferiority, two-arm parallel randomized controlled trial involved treatment-seeking adults with prescription-type opioid use disorder. Participants were randomized in a 1:1 ratio to treatment with sublingual buprenorphine/naloxone (target dosage, 8 mg/2 mg to 24 mg/6 mg per day; flexible take-home dosing) or oral methadone (≈60-120 mg/day; closely supervised). The primary outcome was the proportion of opioid-free urine drug screens over 24 weeks (noninferiority margin, 15%). All randomized participants were analyzed, excluding one who died shortly after randomization, for the primary analysis (modified intention-to-treat analysis). RESULTS Of 272 participants recruited (mean age, 39 years [SD=11]; 34.2% female), 138 were randomized to buprenorphine/naloxone and 134 to methadone. The mean proportion of opioid-free urine drug screens was 24.0% (SD=34.4) in the buprenorphine/naloxone group and 18.5% (SD=30.5) in the methadone group, with a 5.6% adjusted mean difference (95% CI=-0.3, +∞). Participants in the buprenorphine/naloxone group had 0.47 times the odds (95% CI=0.24, 0.90) of being retained in the assigned treatment compared with those in the methadone group. Overall, 24 drug-related adverse events were reported (12 in the buprenorphine/naloxone group [N=8/138; 5.7%] and 12 in the methadone group [N=12/134; 9.0%]) and mostly included withdrawal, hypogonadism, and overdose. CONCLUSIONS The buprenorphine/naloxone flexible model of care was safe and noninferior to methadone in reducing opioid use among people with prescription-type opioid use disorder. This flexibility could help expand access to opioid agonist therapy and reduce harms in the context of the opioid overdose crisis.
Prediction Accuracy Between Terminally Ill Patients’ Survival Length and the Estimations Made From Different Medical Staff, a Prospective Cohort Study

Background: Previous reports suggested the clinical predictions of survival (CPS) and prognostic scores had similar accuracy in patients with days to weeks of life. Objective: We aimed to evaluate and compare the accuracy of CPS by attending physicians, residents, and nurses in an acute palliative care unit at a medical center. Methods: This was a 1-year prospective cohort study. Survival prediction was made within 3 days after patients’ admission and re-evaluated every week until patients’ discharge or death. Associated factors of accurate survival predictions were also explored by multivariate logistic regression. Results: A total of 179 inpatients were recruited and 115 of them were included in this analysis. The mean age of participants was 72.9 years and the average length of actual survival was 11.5 ± 12.0 days. For patients with survival within 30 days, the medical staff tended to overestimate their life span. The predictions made by physicians and nurses showed much closer to actual survival length through repeated estimations. Patients with metastatic cancer (odds ratio: OR 2.77, 95% CI 1.23-6.22) or cognitive impairment (OR 2.39, 95% CI 1.12-5.11) had higher associations with accurate CPS. Poor performance status of ECOG (OR 1.82, 95% CI 1.09-3.02) and dysphagia (OR 2.01, 95% CI 1.07-3.77) were significant predictors for accurate CPS in patients with the survival of less than 2 weeks. Conclusions: The accuracy of CPS between different medical staff did not reveal significant differences in the study. The importance of re-evaluation for patients’ survival length in clinical practice is worthy of attention.
Acceleration and deceleration demands during training sessions in football: a systematic review

ABSTRACT Purpose The aim of this review is to summarize the current scientific knowledge about acceleration and deceleration demands during football training. Methods A systematic search of three electronic databases (PubMed, SPORTDiscus, Web of Science) was performed to identify peer-reviewed relevant English-language articles, following PRISMA guidelines. Results All acceleration and deceleration data were analyzed and organized into four categories: i) training drills variables (i.e. manipulated drills variables such as number of players in small-sided games), ii) training exercises (i.e. different drills such small games or circuit training), iii) players’ positions (i.e. demands for each playing position) and iv) training schedule (i.e. training sessions presented as microcycles, season sections or full season). Full-text articles of 42 studies were included in the final analysis. Players’ level included: amateur, youth, semi-professional, professional and elite players. All playing positions were considered, including goalkeepers. Six different global position systems brands were used, with the majority measuring data at 10 Hz. Different thresholds and intensities were used in several papers. Lower acceleration and deceleration intensities occurred more often than higher intensities in all four categories. Conclusion Different exercises elicit different demands and small-sided games presented higher acceleration and deceleration demands than circuit training and other running based drills. Furthermore, manipulating drills variables, as reducing or increasing number of players in small-sided games increase or decrease demands, respectively. Additionally, wide playing positions, such as fullbacks, are generally exposed to higher acceleration and deceleration demands. From a planning point of view, acceleration and deceleration demands decrease as match day approaches.
The Effectiveness of A Slow-Paced Diaphragmatic Breathing Exercise in Children’s Daily Life: A Micro-Randomized Trial

ABSTRACT Objective Breathing exercises have been proposed as an effective intervention to improve subjective well-being and manage anxiety symptoms. As they are comparatively easy to learn and to implement, breathing exercises may be particularly beneficial for children. Although breathing exercises are ultimately supposed to provide salutary effects in individuals’ everyday lives, immediate effects of breathing exercises in naturalistic contexts have received limited empirical attention. The purpose of this study was to examine immediate effects of slow-paced diaphragmatic breathing on negative affect as well as on relaxation in an ecologically valid setting. To that end, we conducted a micro-randomized trial in children’s daily life. Method On each of 15 days, children (N = 171, aged 9–13 years, 54% female) were randomized to different conditions: performing a video-guided slow-paced diaphragmatic breathing exercise (experimental condition), watching a different video (active control condition), or a passive control condition. Results The breathing exercise had no immediate effects on negative affect or relaxation compared to both control conditions. However, in situations when children reported higher levels of worries than usual, relaxation was higher when children performed the breathing exercise compared to the passive control condition. Compared to the active control condition, the breathing exercise did not result in higher levels of relaxation in situations when children worried more than normally. Conclusions Findings highlight that context-specific factors can modulate the effectiveness of breathing exercises and should be taken into account to tailor interventions to individuals’ needs.
Lattice Quantum ChromoDynamics with approximately chiral fermions

In this work we present Lattice QCD results obtained by approximately chiral fermions. We use the CI fermions in the quenched approximation to investigate the excited baryon spectrum and to search for the Theta^+ pentaquark on the lattice. Furthermore we developed an algorithm for dynamical simulations using the FP action. Using FP fermions we calculate some LECs of chiral perturbation theory applying the epsilon expansion.
Design, Synthesis and Anti‐Tumor Activity Evaluation of Novel 3,4‐(Methylenedioxy)cinnamic Acid Amide‐Dithiocarbamate Derivatives

The fragments, 3,4‐(methylenedioxy)cinnamic acid amide and dithiocarbamates, have received increasing attention because of their multiple pharmacological activities in recent years, especially in anti‐tumor. We synthesized 17 novel 3,4‐(methylenedioxy)cinnamic acid amide‐dithiocarbamate derivatives based on the principle of pharmacophore assembly and discovered that compound 4a7 displayed the most potent antiproliferative activity against HeLa cells with IC50 value of 1.01 μM. Further mechanistic studies revealed that 4a7 triggered apoptosis in HeLa cells via activating mitochondria‐mediated intrinsic pathways and effectively inhibited colony formation. Also, 4a7 had the ability to arrest cell cycle in the G2/M phase as well as to inhibit the migration in HeLa cells. More importantly, acute toxicity experiments showed that 4a7 had good safety in vivo. All the results suggested that compound 4a7 might serve as a promising lead compound that merited further attention in future anti‐tumor drug discovery.
African American Gatekeepers or the Black Church?: Using Modified Grounded Theory to Explore the Debate on Black Homophobia

ABSTRACT In a comparison of attitudes concerning same-sex relationships and inclusive policies for lesbian, gay, bisexual, transgender, and intersex (LGBTI) persons, African Americans are more likely to possess stronger opposition than Whites. The default agent in explaining disapproving attitudes is the Black Church. However, observations from 2017 expert interviews, part of a study on causes of Black homophobia, revealed that varying experts do not affirm the Black Church as the primary actor in sustaining these attitudes. Based on this observation, this study theorizes that attitudes considered homophobic are primarily sustained by actors distinct and separate from the Black Church. This study employs a modified grounded theory to explore themes toward the creation of an expert-driven narrative that disapproval of same-sex relationships and opposition to LGBTI policies are attitudes sustained by Black gatekeepers, as they see same-sex relationships and policy as oppositional to the Black identity and Black sociopolitical progress.
Genetic diversity of two Portuguese populations of the pullet carpet shell , based on RAPD markers: contribution to a sustainable restocking program

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Genetic diversity of two Portuguese populations of the pullet carpet shell , based on RAPD markers: contribution to a sustainable restocking program Sandra Joaquim, Jorge Pereira, Alexandra Leitão, Domitília Matias, Raquel Chaves, Henrique Guedes-Pinto, Luís Chícharo, Miguel Gaspar
ReviewCurrent State and Perspectives in Population Genomics of the Common BeanGaia Cortinovis

Population genomics integrates advances in sequencing technologies, bioinformatics tools, statistical methods and software into research on evolutionary and population genetics. Its application has provided novel approaches that have significantly advanced our understanding of new and long-standing questions in evolutionary processes. This has allowed the disentangling of locus-specific effects from genome-wide effects and has shed light on the genomic basis of fitness, local adaptation and phenotypes. “-Omics” tools have provided a comprehensive genome-wide view of the action of evolution. The specific features of the Phaseolus genus have made it a unique example for the study of crop evolution. The well-documented history of multiple domestications in Phaseolus vulgaris L. (common bean) and its further adaptation to different environments have provided the opportunity to investigate evolutionary issues, such as convergent evolution in the same species across different domestication events. Moreover, the availability of the P. vulgaris reference genome now allows adaptive variations to be easily mapped across the entire genome. Here, we provide an overview of the most significant outcomes obtained in common bean through the use of different computational tools for analysis of population genomics data.
Site-specific glycations of Aβ1–42 affect fibril formation and are neurotoxic

Amyloid β1–42 (Aβ1–42) peptide is involved in Alzheimer’s disease (AD) pathogenesis and is prone to glycation, an irreversible process resulting in proteins with accumulated advanced glycated end products (AGEs). Nε(carboxyethyl)lysine (CEL) is a common ADassociated AGE, occurring at either Lys-16 or Lys-28 of Aβ1–42. Methylglyoxal is commonly used for the unspecific glycation of Aβ1–42, which results in a complex mixture of AGEmodified peptides and makes interpretation of a causative AGE at a specific amino acid residue difficult. Here, we addressed this challenge by chemically synthesizing defined CEL glycations on Aβ1–42 at Lys-16 (Aβ-CEL16), Lys-28 (AβCEL28), and both Lys-16 and -28 (AβCEL16&28). We demonstrate that the double CEL glycation at Lys-16/28 of Aβ1–42 had the most profound impact on amyloid fibril formation. In silico predictions indicated that AβCEL16&28 had a substantially decreased freeenergy change, contributing to fibril destabilization, and a decreased aggregation rate. Single CEL glycations at Lys-28 had the least impact on fibril formation, and Lys-16 CEL glycations delayed fibril formation. We also tested these peptides for neuronal toxicity and impact on mitochondrial function in a retinoic acid–differentiated SH-SY5Y human neuroblastoma cell line and found that only AβCEL16 and Aβ-CEL28 are neurotoxic, possibly through a non-mitochondrial pathway, whereas Aβ-CEL16&28 is not neurotoxic. Interestingly, Aβ-CEL16&28 depolarized the mitochondrial membrane potential, and Aβ-CEL16 increased mitochondrial respiration at complex II, possibly indicating mitophagy or an alternative metabolic route, respectively. In summary, our results provide insights relevant for potential therapeutic approaches against neurotoxic CEL glycated Aβ1–42. Aβ1–42 peptides are implicated in the pathogenesis of Alzheimer’s disease (AD). Aβ1– 42 peptides are released extracellularly after being enzymatically processed from the amyloid http://www.jbc. org/cgi/doi/10.1074/jbc.RA118.006846 The latest version is at JBC Papers in Press. Published on April 17, 2019 as Manuscript RA118.006846 by gest on A ril 7, 2019 hp://w w w . jb.org/ D ow nladed from
PPA_A_265958 1477..1492

1The School of Health and Biomedical Sciences, RMIT University, Melbourne, VIC, Australia; 2School of Science, Psychology and Sport, Federation University, Melbourne, VIC, Australia; 3Institute for Breathing and Sleep, Austin Health, Melbourne, VIC, Australia Objective: The purpose of this review was to evaluate the modalities (e.g., face-to-face, telephone or electronic) of pharmacist health coaching providing the greatest improvement in patient outcomes, to enable a more comprehensive evaluation to be done and quality decision-making around health coaching modalities to be undertaken by pharmacists. Methods: This systematic review followed the PRISMA guidelines. CINHAL, EMBASE, PubMed, PsychINFO and SCOPUS were searched (2000–2019). Included articles were reviewed for the modality used to health coach, the training provided, and the outcomes. Results: Twelve papers met the eligibility criteria. A majority of studies included involved a combination of modalities of pharmacist health coaching. Four papers referred to face-toface sessions, and one study used telephone coaching. In each paper, coaching led to an improvement in clinical and non-clinical health outcomes. Conclusion: The training provided to health coaches varied and in some cases was not reported. Inconsistencies in reports led to difficulties when comparing study outcomes. Therefore, conclusions about the modality providing the greatest improvement in patient outcomes and the most pragmatic health coaching modality are not possible. Studies that document the training, the modality, the outcomes and the cost benefits of coaching by pharmacists are warranted to enable a more comprehensive evaluation to be done and quality decision-making around health coaching modalities to be undertaken by pharmacists.
PRBM_A_326660 2035..2043

1Convergence Innovation Support Center, Gangwon Technopark, Chuncheon, South Korea; 2Department of Health Policy and Management, Sangji University, Wonju, South Korea Purpose: This study identified the relationships between perceived household economic status and household economic downturn due to COVID-19 and adolescent depressive symptoms and suicidal ideation. Methods: Participants for this study were extracted from the 13th Korea Youth Risk Behavior Web-Based Survey, conducted from August to November 2020. The participants comprised 54,948 middle and high school students selected by stratified random cluster sampling. Results: The prevalence rates of depressive symptoms and suicidal ideation were 25.2% and 10.9%, respectively. Multivariate logistic regression analysis showed that lower perceived household economic status significantly predicted higher prevalence of depressive symptoms and suicidal ideation. Participants who perceived that their household economic status had declined because of COVID-19 were more likely to have experienced depression and suicidal ideation. These results were similar regardless of the participants’ perceptions of household economic status. Conclusion: This study found that in the ongoing pandemic, there is a need for an active mental health promotion program for adolescents from low-income households, especially those who experienced a recent decline in the household economy.
Cognitive ability in male and female medical students

In matriculation examinations at age 16, girls achieved greater score than boys. It is still uncertain whether gender gap in examination achievement reflects sex difference in fundamental cognitive domains or not. This cross sectional comparative study was undertaken to determine the cognitive ability in 62 male and 62 female medical students between 16-22 years of age attending in University of Medicine (2) Yangon with body mass index within 18.5-24.9 kg/m. Blood haemoglobin concentration was determined by cyamet-haemoglobin method. Serum ferritin concentration was measured by enzyme linked immunoassay method. Cognitive ability, i.e. four main cognitive domains; attention, memory and learning is assessed by using General Memory Scale, and reasoning is assessed by Raven’s Standard Progressive Matrices. Blood haemoglobin concentration and serum ferritin concerntration were 14.17 ± 1.04 g/dl, 61.78 ± 35.24 μg/L in male and 12.85 ± 1.09 g/dl, 38.77 ± 25.79 μg/L in female, respectively. They are within normal range. General memory score for male was 60.38 ± 10.02 and for female was 58.30 ± 8.01 (P = 0.206). Raven’s progressive matrices score were 41.62 ± 7.35 and 43.62 ± 7.35 in male and female, respectively (P = 0.133). Mental control score for male was 5.09 ± 1.76 and for female was 5.08 ± 1.48 (P = 0.956). Logical memory score for male was 18.56 ± 5.35 and female was 18.19 ± 5.13 (P = 0.694), Digit span score for male was 13.37 ± 1.42 and female was 13.20 ± 1.26 (P = 0.508), Visual memory score for male was 5.62 ± 0.46 and female was 5.58 ± 0.42 (P = 0.619), Paired associative learning score for male was 17.34 ± 6.12 and female was 16.24 ± 5.34 (P = 0.286). There were no significant differences in score of mental control, logical memory, digit span, visual memory and paired associative learning, General Memory Score and Raven’s standard progressive matrices. These results showed no significant difference in cognitive ability between male and female medical students.