Miracle-dz/UniFAR-FTUs huggingface dataset:
A dual synergistic effect of titanium and curcumin co-embedded on extracellular matrix hydrogels of decellularized bone: Potential application in osteoblastic differentiation of adipose-derived mesenchymal stem cells

Abstract This research aims to design and fabricate a novel hydrogel-based composite as a functional biomimetic and biocompatible scaffold for amended osteoblastic differentiation of adipose-derived mesenchymal stem cells (ADMSCs). The extracellular matrix (ECM) hydrogel is an ideal scaffold in tissue engineering in terms of its structure mimics natural tissue. In this study, the fresh bovine femur was demineralized and decellularized; next, ECM hydrogel was obtained by digesting these matrices. Then, TiO2 and curcumin-loaded hydrogel (Hy/Ti/Cur) was fabricated besides TiO2-loaded hydrogel (Hy/Ti) and curcumin-loaded hydrogel (Hy/Cur). Comparing the scanning electron microscopy (SEM) images of the pure network hydrogel and the rough morphology of Hy/Ti/Cur revealed that curcumin and titanium dioxide were successfully loaded into the hydrogel. In addition, FTIR spectroscopy and X-ray diffraction (XRD) validated these findings. The findings of the hydrogels’ swelling test indicated the favourable impact of curcumin and titanium dioxide in hydrogels, which enhances water absorption capacity. Our results showed that the hydrogels were cytocompatible, and the cell viability on the hydrogels was elevated compared to the control. The synergistic effect of TiO2 and Cur co-embedded on ECM hydrogel (Hy/Ti/Cur) stimulates bone differentiation markers, such as Runt-related transcription factor 2 (RUNX-2) and osteocalcin (OCN) in ADMSCs cultured in normal and osteogenic medium. Moreover, Alkaline Phosphatase (ALP) activity and calcium deposition of ADMSCs cultured on engineered hydrogels were increased. These experiments showed that newly fabricated hydrogel has the potential to induce osteogenesis, which is recommended as an attractive scaffold in bone tissue engineering.
Association between physician's case volume in prehospital advanced trauma care and 30-day mortality: A registry-based analysis of 4,032 patients

New study suggests that organizing advanced prehospital trauma care in a way that ensures physicians encounter trauma patients frequently might improve patient survival. BACKGROUND Seriously injured patients may benefit from prehospital interventions provided by a critical care physician. The relationship between case volume and outcome has been established in trauma teams in hospitals, as well as in prehospital advanced airway management. In this study, we aimed to assess if a volume-outcome relationship exists in prehospital advanced trauma care. METHODS We performed a retrospective cohort study using the national helicopter emergency medical services database, including trauma patients escorted from scene to hospital by a helicopter emergency medical services physician during January 1, 2013, to August 31, 2019. In addition, similar cases during 2012 were used to determine case volumes. We performed a multivariate logistic regression analysis, with 30-day mortality as the outcome. Age, sex, Glasgow Coma Scale, shock index, mechanism of injury, time interval from alarm to the patient and duration of transport, level of receiving hospital, and physician's trauma case volume were used as covariates. On-scene times, interventions performed, and status at hospital arrival were assessed in patients who were grouped according to physician's case volume. RESULTS In total, 4,032 escorted trauma patients were included in the study. The median age was 40.2 (22.9–59.3) years, and 3,032 (75.2%) were male. Within 30 days, 498 (13.2%) of these patients had died. In the highest case volume group, advanced interventions were performed more often, and patients were less often hypotensive at handover. Data for multivariate analysis were available for 3,167 (78.5%) of the patients. Higher case volume was independently associated with lower mortality (odds ratio, 0.59; 95% confidence interval, 0.38–0.89). CONCLUSION When a prehospital physician's case volume is higher in high-risk prehospital trauma, this seems to be associated with more active practice patterns and significantly lower 30-day mortality. The quality of prehospital critical care could be increased by ensuring sufficient case volume for the providers of such care. LEVEL OF EVIDENCE Prognostic and Epidemiologic; Level III.
De novo assembly and annotation of the CHOZN® GS−/− genome supports high‐throughput genome‐scale screening

Chinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial‐scale production processes and media, the CHO cell has remained largely unchanged. Due to the cost and complexity of whole‐genome sequencing and gene‐editing it has been difficult to obtain the tools necessary to improve the CHO cell line. With the advent of next‐generation sequencing and the discovery of the CRISPR/Cas9 system it has become more cost effective to sequence and manipulate the CHO genome. Here, we provide a comprehensive de novo assembly and annotation of the CHO‐K1 based CHOZN® GS−/− genome. Using this platform, we designed, built, and confirmed the functionality of a whole genome CRISPR guide RNA library that will allow the bioprocessing community to design a more robust CHO cell line leading to the production of life saving medications in a more cost‐effective manner.
Multi3: multi-templates siamese network with multi-peaks detection and multi-features refinement for target tracking in ultrasound image sequences

Objective. Radiation therapy requires a precise target location. However, respiratory motion increases the uncertainties of the target location. Accurate and robust tracking is significant for improving operation accuracy. Approach. In this work, we propose a tracking framework Multi3, including a multi-templates Siamese network, multi-peaks detection and multi-features refinement, for target tracking in ultrasound sequences. Specifically, we use two templates to provide the location and deformation of the target for robust tracking. Multi-peaks detection is applied to extend the set of potential target locations, and multi-features refinement is designed to select an appropriate location as the tracking result by quality assessment. Main results. The proposed Multi3 is evaluated on a public dataset, i.e. MICCAI 2015 challenge on liver ultrasound tracking (CLUST), and our clinical dataset provided by the Chinese People’s Liberation Army General Hospital. Experimental results show that Multi3 achieves accurate and robust tracking in ultrasound sequences (0.75 ± 0.62 mm and 0.51 ± 0.32 mm tracking errors in two datasets). Significance. The proposed Multi3 is the most robust method on the CLUST 2D benchmark set, exhibiting potential in clinical practice.
Imposter Syndrome Among Surgeons Is Associated With Intolerance of Uncertainty and Lower Confidence in Problem Solving

Abstract Background Feelings of imposter syndrome (inadequacy or incompetence) are common among physicians and are associated with diminished joy in practice. Identification of modifiable factors associated with feelings of imposter syndrome might inform strategies to ameliorate them. To this point, though, no such factors have been identified. Question/purpose Are intolerance of uncertainty and confidence in problem-solving skills independently associated with feelings of imposter syndrome after accounting for other factors? Methods This survey-based experiment measured the relationship between feelings of imposter syndrome, intolerance of uncertainty, and confidence in problem-solving skills among musculoskeletal specialist surgeons. Approximately 200 surgeons who actively participate in the Science of Variation Group, a collaboration of mainly orthopaedic surgeons specializing in upper extremity illnesses primarily across Europe and North America, were invited to this survey-based experiment. One hundred two surgeons completed questionnaires measuring feelings of imposter syndrome (an adaptation of the Clance Imposter Phenomenon Scale), tolerance of uncertainty (the Intolerance of Uncertainty Scale-12), and confidence in problem-solving skills (the Personal Optimism and Self-Efficacy Optimism questionnaire), as well as basic demographics. The participants were characteristic of other Science of Variation Group experiments: the mean age was 52 ± 5 years, with 89% (91 of 102) being men, most self-reported White race (81% [83 of 102]), largely subspecializing in hand and/or wrist surgery (73% [74 of 102]), and with just over half of the group (54% [55 of 102]) having greater than 11 years of experience. We sought to identify factors associated with greater feelings of imposter syndrome in a multivariable statistical model. Results Accounting for potential confounding factors such as years of experience or supervision of trainees in the multivariable linear regression analysis, greater feelings of imposter syndrome were modestly associated with higher intolerance of uncertainty (regression coefficient [β] 0.34 [95% confidence interval (CI) 0.16 to 0.51]; p < 0.01) and with lower confidence in problem-solving skills (β -0.70 [95% CI -1.0 to -0.35]; p < 0.01). Conclusion The finding that feelings of imposter syndrome may be modestly to notably associated with modifiable factors, such as difficulty managing uncertainty and lack of confidence in problem-solving, spark coaching opportunities to support and sustain a surgeon’s mindset, which may lead to increased comfort and joy at work. Clinical Relevance Beginning with premedical coursework and throughout medical training and continuing medical education, future studies can address the impact of learning and practicing tactics that increase comfort with uncertainty and greater confidence in problem solving on limiting feelings of imposter syndrome.
Analysis of Cross-sectional and Longitudinal HLA and Anti-viral Responses After COVID Infection in Renal Allograft Recipients: Differences and Correlates

Background. Characterization of anti-HLA versus anti-severe acute respiratory syndrome coronavirus 2 (anti–SARS-CoV-2) immune globulin isotypes in organ transplant recipients after coronavirus disease 2019 (COVID-19) infection has not been reported. We aimed to determine changes in anti-HLA antibodies in renal transplant patients with COVID-19 and compare the immunoglobulin and epitope-binding pattern versus anti–SARS-CoV-2 antibodies. Methods. This is a cross-sectional study of 46 kidney transplant recipients including 21 with longitudinal sampling. Using a semi-quantitative multiplex assay, we determined immunoglobulin (Ig) M, IgA, IgG, and IgG1-2-3-4 antibodies against Class I and Class II HLA, and 5 SARS-CoV-2 epitopes including the nucleocapsid protein and multiple regions of the spike protein. Results. Fourteen of 46 (30%) patients had donor-specific anti-HLA antibodies (donor-specific antibody [DSA]), 12 (26%) had non-DSA anti-HLA antibodies and 45 (98%) had anti–SARS-CoV-2 antibodies. Most DSAs targeted HLA-DQ (71%), with a dominant IgG isotype and IgG1 subtype prevalence (93%), and/or IgG3 (64%), followed by IgG2 (36%). Comparatively, there was a higher prevalence of IgA (85% versus 14%, P = 0.0001) and IgM (87%, versus 36%, P = 0.001) in the anti–SARS-CoV-2 antibody profile, when compared to DSAs, respectively. Anti–SARS-CoV-2 antibody profile was characterized by increased prevalence of IgM and IgA, when compared to DSAs. The median calculated panel reactive antibody before COVID-19 diagnosis (24%) tended to decrease after COVID-19 diagnosis (10%) but it was not statistically significant (P = 0.1). Conclusions. Anti-HLA antibody strength and calculated panel reactive antibody in kidney transplant recipients after COVID-19 do not significantly increase after infection. Although the IgG isotype was the dominant form in both HLA and SARS-CoV-2 antigens, the alloimmune response had a low IgA pattern, whereas anti–SARS-CoV-2 antibodies were high IgA/IgM.
Yinjia pill inhibits persistent Chlamydia trachomatis infection

To the Editor: Chlamydia trachomatis (C. trachomatis) is obligate intracellular pathogens, often resulting in development of chronic disease and tissue damage as a consequence of the host inflammatory immune response as well as persistent infections, which is associated with the activation of mitogen-activated protein kinase (MAPK) and extracellular regulated protein kinases (ERK) mediated by Toll-like receptors (TLRs) and myeloid differentiation primary response gene 88 (MyD88). Furthermore, C. trachomatis has evolved the ability to block the apoptosis of its host cells involving the interaction of autophagy and apoptosis. Novel strategies to prevent and treat C. trachomatis infection effectively are needed.
The Remarkable Underlying Ground States of Cuprate Superconductors

Cuprates exhibit exceptionally strong superconductivity. To understand why, it is essential to elucidate the nature of the electronic interactions that cause pairing. Superconductivity occurs on the backdrop of several underlying electronic phases, including a doped Mott insulator at low doping, a strange metal at high doping, and an enigmatic pseudogap phase in between—inside which a phase of charge-density wave order appears. In this article, we shed light on the nature of these remarkable phases by focusing on the limit as [Formula: see text], where experimental signatures and theoretical statements become sharper. We therefore survey the ground-state properties of cuprates once superconductivity has been removed by the application of a magnetic field and distill their key universal features.
Feature-based Image Comparison and Its Application in Wireless Visual Sensor Networks

This dissertation studies the feature-based image comparison method and its application in Wireless Visual Sensor Networks. Wireless Visual Sensor Networks (WVSNs), formed by a large number of low-cost, small-size visual sensor nodes, represent a new trend in surveillance and monitoring practices. Although each single sensor has very limited capability in sensing, processing and transmission, by working together they can achieve various high level tasks. Sensor collaboration is essential to WVSNs and normally performed among sensors having similar measurements, which are called neighbor sensors. The directional sensing characteristics of imagers and the presence of visual occlusion present unique challenges to neighborhood formation, as geographically-close neighbors might not monitor similar scenes. Besides, the energy resource on the WVSNs is also very tight, with wireless communication and complicated computation consuming most energy in WVSNs. Therefore the feature-based image comparison method has been proposed, which directly compares the captured image from each visual sensor in an economical way in terms of both the computational cost and the transmission overhead. The feature-based image comparison method compares different images and aims to find similar image pairs using a set of local features from each image. The image feature is a numerical representation of the raw image and can be more compact in terms of the data volume than the raw image. The feature-based image comparison contains three steps: feature detection, descriptor calculation and feature comparison. For the step of feature detection, the dissertation proposes two computationally efficient corner detectors. The first detector is based on the Discrete Wavelet Transform that provides multi-scale corner point detection and the scale selection is achieved efficiently
Health benefits of virgin coconut oil

Introduction Virgin coconut oil is extracted directly from fresh coconut meat. There are several techniques being used which can be classified generally into: (1) drying the freshly grated coconut meat at low temperature, no higher than 60C, followed by pressing to extract the oil, (2) by extracting the coconut milk from the freshly grated coconut meat, followed by the addition of enzyme or aging for several hours, or by mechanical process using continuous centrifuge. To safeguard the quality of virgin coconut oil (VCO), it is important that moisture level must be kept at the minimum. Philippine National Standard for Virgin Coconut Oil specifies moisture and volatile matters content at 0.2% maximum. Additionally, it describes the oil as colorless, sediment free, with natural fresh coconut scent, and free from rancid odor or taste. Coconut oil, although the most stable oil being highly saturated, still
The number of subspaces occurring in the p-adic subspace theorem in diophantine approximation.

Unfortunately the underlying method of Thue-Siegel-Roth is ineffective in the sense that it does not provide bounds for the sizes of χ and y. However it does give explicit upper bounds for the number of such approximations. In fact in a paper by Davenport and Roth [3] it was shown that this number is under some bound which depends only upon d, δ and the height //0 = /f0(a), the maximum modulus of the coefficients of the minimal defining polynomial of α over Z. Bombieri and van der Poorten [1] and independently Luckhardt [6] using the modified proof of Roth's Theorem s presented by Esnault and Viehweg [4] obtained much better bounds.
OBSERVATIONS OF TYPE Ia SUPERNOVA 2014J WITH FLITECAM ON SOFIA

We present medium-resolution near-infrared (NIR) spectra, covering 1.1–3.4 μm, of the normal Type Ia supernova (SN Ia) SN 2014J in M82 obtained with the FLITECAM instrument on board Stratospheric Observatory for Infrared Astronomy (SOFIA) between 17 and 26 days after maximum B light. Our 2.8–3.4 μm spectra may be the first ∼3 μm spectra of an SN Ia ever published. The spectra spanning the 1.5–2.7 μm range are characterized by a strong emission feature at ∼1.77 μm with a FWHM of ∼11,000–13,000 km s−1. We compare the observed FLITECAM spectra to the recent non-LTE delayed detonation models of Dessart et al. and find that the models agree with the spectra remarkably well in the 1.5–2.7 μm wavelength range. Based on this comparison we identify the ∼1.77 μm emission peak as a blend of permitted lines of Co ii. Other features seen in the 2.0–2.5 μm spectra are also identified as emission from permitted transitions of Co ii. However, the models are not as successful at reproducing the spectra in the 1.1–1.4 μm range or between 2.8 and 3.4 μm. These observations demonstrate the promise of SOFIA, which allows access to wavelength regions inaccessible from the ground, and serve to draw attention to the usefulness of the regions between the standard ground-based NIR passbands for constraining SN models.
BOUNDARY LAYER TRANSITION ON CONES AND SPHERES AT SUPERSONIC SPEEDS-EFFECTS OF ROUGHNESS AND COOLING,

Abstract : Results of experiments on boundary layer transition as affected by single roughness elements on cones and spheres in supersonic flow are reviewed and analyzed. Results for the cone includes cooling, whereupon it is found that cooling decreases transition roughness size linearly with wall temperature. The sphere was uncooled, however the curvature of the surface had to be taken into account to collapse the data for various sized roughness elements. Relationships are presented which correlate roughness, Mach number, cooling, and body curvature effects on transition. (Author)
Isolated spectral points

The paper studies isolated spectral points of elements of Banach algebras and of bounded linear operators in terms of the existence of idempotents, and gives an elementary characterization of spectral idempotents. It is shown that 0 is isolated in the spectrum of a bounded linear operator T if the (not necessarily closed) space M = {x: lim, JHTn XII/n 0} is nonzero and complemented by a closed subspace N satisfying TN C N C TX.
Students' Facebook Usage and Academic Achievement: A Case Study of Private University in Thailand.

The objective of this study was to determine if the time spent on Facebook and the purpose for which Facebook was used had any impact on the academic achievement of the students. This exploratory research used a questionnaire to collect data from 251 undergraduate students at a private university in Bangkok, Thailand. Data were analyzed using Multiple Regression Analysis to the conclusion that students used Facebook on an average of one hour and thirty minutes, for the purposes of communication and collaboration; that the number of hours spent on Facebook had no effect on their academic achievement; that using Facebook for communication impaired academic achievement; and, that using Facebook for collaboration did not impair academic achievement. The study has also found that time management is a key predictor in the determination of the students’ academic achievement.
Canonical bases of higher-level q-deformed Fock spaces

We define canonical bases of the higher-level q-deformed Fock space modules of the affine Lie algebra sl(n)^. This generalizes the result of Leclerc and Thibon for the case of level 1. We express the transition matrices between the canonical bases and the natural bases of the Fock spaces in terms of affine Kazhdan-Lusztig polynomials.
A 3 pc SCALE JET-DRIVEN OUTFLOW FROM SGR A*

The compact radio source Sgr A* is coincident with a 4 × 106 M☉ black hole at the dynamical center of the Galaxy and is surrounded by dense orbiting ionized and molecular gas. We present high-resolution radio continuum images of the central 3′ and report a faint continuous linear structure centered on Sgr A* with a P.A. ∼ 60°. The extension of this feature appears to be terminated symmetrically by two linearly polarized structures at 8.4 GHz, ∼75″ from Sgr A*. A number of weak blobs of radio emission with X-ray counterparts are detected along the axis of the linear structure. The linear structure is best characterized by a mildly relativistic jet from Sgr A* with an outflow rate 10−6 M☉ yr−1. The near and far sides of the jet are interacting with orbiting ionized and molecular gas over the last 1–3 hundred years and are responsible for a 2″ hole, the “minicavity,” characterized by disturbed kinematics, enhanced Fe ii/iii line emission, and diffuse X-ray gas. The estimated kinetic luminosity of the outflow is ∼1.2 × 1041 erg s−1, so the interaction with the bar may be responsible for the Galactic center X-ray flash inferred to be responsible for much of the fluorescent Fe Kα line emission from the inner 100 pc of the Galaxy.
Shoulder Surfing through the Social Lens: A Longitudinal Investigation & Insights from an Exploratory Diary Study

Shoulder surfing is a prevailing threat when accessing information on personal devices like smartphones. Adequate mitigation requires studying shoulder surfing occurrences in people’s daily lives. In this paper, we confirm and extend previous research findings on shoulder surfing occurrences using a new method; a one-month diary study (N=23). Our results provide evidence of shoulder surfing in public and private environments. Content-based shoulder surfing happens more frequently than authentication-based shoulder surfing. Participants experienced shoulder surfing at least twice during the study period and considered the closeness of relationships with the shoulder surfers when deciding how to respond to shoulder surfing incidents. Participants preferred unobtrusive alerting mechanisms over mitigation mechanisms for protection against shoulder surfing. Our work advocates moving away from one-size-fits-all privacy solutions and supports the design of user-centred shoulder surfing mitigation methods that consider social aspects. We conclude with directions for future research to assist security researchers and practitioners.