Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression Pattern of Purinergic Signaling Components in Colorectal Cancer Cells and Differential Cellular Outcomes Induced by Extracellular ATP and Adenosine

The purine nucleotide adenosine triphosphate (ATP) is known for its fundamental role in cellular bioenergetics. However, in the last decades, different works have described emerging functions for ATP, such as that of a danger signaling molecule acting in the extracellular space on both tumor and stromal compartments. Beside its role in immune cell signaling, several studies have shown that high concentrations of extracellular ATP can directly or indirectly act on cancer cells. Accordingly, it has been reported that purinergic receptors are widely expressed in tumor cells. However, their expression pattern is often associated with contradictory cellular outcomes. In this work, we first investigated gene expression profiles through “RNA-Sequencing” (RNA Seq) technology in four colorectal cancer (CRC) cell lines (HT29, LS513, LS174T, HCT116). Our results demonstrate that CRC cells mostly express the A2B, P2X4, P2Y1, P2Y2 and P2Y11 purinergic receptors. Among these, the P2Y1 and P2Y2 coding genes are markedly overexpressed in all CRC cells compared to the HCEC-1CT normal-like colonic cells. We then explored the cellular outcomes induced by extracellular ATP and adenosine. Our results show that in terms of cell death induction extracellular ATP is consistently more active than adenosine against CRC, while neither compound affected normal-like colonic cell survival. Intriguingly, while for the P2Y2 receptor pharmacological inhibition completely abolished the rise in cytoplasmic Ca2+ observed after ATP exposure in all CRC cell lines, Ca2+ mobilization only impacted the cellular outcome for HT29. In contrast, non-selective phosphodiesterase inhibition completely abolished the effects of extracellular ATP on CRC cells, suggesting that cAMP and/or cGMP levels might determine cellular outcome. Altogether, our study provides novel insights into the characterization of purinergic signaling in CRC.
Structural, Theoretical and Biological Studies of (Z)-3-Amino-N-(3-Amino Pyrazine-2-Carbonyl) Pyrazine-2-Carbohydrazonic Acid (APA; L) and Its Cu2+, Co2+, Pt4+ and Pd2+ Chelates

New chelates derived from the novel ligand, (Z)-3-amino-N-(3-amino pyrazine-2-carbonyl)pyrazine-2-carbohydrazonic acid (APA, L), with Cu 2+ , Co 2+ , Pt 4+ and Pd 2+ salts were investigated. The results suggest that APA acts as mononegative tridentate in the case of Cu 2+ , binegative tetradentate in the case of Co 2+ and as mononegative bidentate towards Pt 4+ and Pd 2+ chelates. The results of the corrected µ eff . and spectral suggest the structures of the isolated chelates. The results of the corrected µ eff . and spectral suggest the geometries of the isolated chelates. Molecular modeling is deduced and chemical reactivity, energy components for chelates and also MEP for APA is illustrated. In Vitro, the SOD and radical scavengers like activity of the synthesized compounds Hep G2 liver cancer cells and cytotoxic activity were checked. Metal chelates show potent anti-oxidative activity. The results of cytotoxic activity assay against hepatocellular carcinoma cell line Hep G2 confirmed that Pt 4+ complex has the highest value, while APA, Cu 2+ , Co 2+ and Pd 2+ chelates have no significant cytotoxic activity.
Gradient Relative Permittivity Superstrate for Decoupling of Two Closely Located Dual-Polarized Slot Antennas

A simple decoupling method between two closely located dual-polarized slot antennas is proposed in this communication. Based on substrate integrated waveguide (SIW), the dual-polarized antenna is realized using cross-shaped slot and U-shaped feed line, and two identical elements are closely located in edge-to-edge space of only <inline-formula> <tex-math notation="LaTeX">$0.031\lambda _{0}$ </tex-math></inline-formula> to form the multiple-input–multiple-output (MIMO) array, where <inline-formula> <tex-math notation="LaTeX">$\lambda _{0}$ </tex-math></inline-formula> is the free-space wavelength at the center operating frequency. Wideband characteristics, high dual-polarized isolation, and low envelope correlation coefficient (ECC) are realized by introducing a superstrate with gradient relative permittivity (GRP) above the array. The GRP is obtained using drilling hole arrays on low-cost FR-4 materials. To validate the proposed design, the antenna array is fabricated and measured. The measured result shows that 13 and 16 dB isolation enhancements are provided for two orthogonally polarized fields, simultaneously. Moreover, the measured operating bandwidth is from 8 to 10.35 GHz (about 25.6%, the isolation is more than 20 dB), and the ECC between two antennas is less than −52 dB for <inline-formula> <tex-math notation="LaTeX">$E_{1}$ </tex-math></inline-formula>-pol and −62.4 dB for <inline-formula> <tex-math notation="LaTeX">$E_{2}$ </tex-math></inline-formula>-pol over entire operating band. The proposed method has the potential to be used in MIMO wireless systems.
Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2

Systemic symptoms have often been observed in patients with coronavirus disease 2019 (COVID-19) in addition to pneumonia, however, the details are still unclear due to the lack of an appropriate animal model. In this study, we investigated and compared blood coagulation abnormalities and tissue damage between male Syrian hamsters of 9 (young) and over 36 (aged) weeks old after intranasal infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite similar levels of viral replication and inflammatory responses in the lungs of both age groups, aged but not young hamsters showed significant prolongation of prothrombin time and prominent acute kidney damage. Moreover, aged hamsters demonstrated increased intravascular coagulation time-dependently in the lungs, suggesting that consumption of coagulation factors causes prothrombin time prolongation. Furthermore, proximal urinary tract damage and mesangial matrix expansion were observed in the kidneys of the aged hamsters at early and later disease stages, respectively. Given that the severity and mortality of COVID-19 are higher in elderly human patients, the effect of aging on pathogenesis needs to be understood and should be considered for the selection of animal models. We, thus, propose that the aged hamster is a good small animal model for COVID-19 research.
Intercomparison of gridded precipitation datasets for prospective hydrological applications in Sri Lanka

The aim of this study was to assess the application of gridded precipitation datasets for applications in Sri Lanka and to identify suitable products that can be used for hydrological applications in data sparse regions. Here, nine global datasets were considered for this study, and after initial screening, four datasets, such as Integrated Multi‐Satellite Retrievals for GPM (IMERG) early run calibrated, Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS), European Center for Medium range Weather Forecasting (ECMWF)—ReAnalysis (ERA5) and Indian Monsoon Data Assimilation and Reanalysis (IMDAA), were selected for detailed comparison. These data products were analysed at monthly, seasonal and annual time scale for whole Sri Lanka and also for three distinguished climate zones (wet, intermediate and dry) for the period 2001–2015. The datasets were further evaluated based on their ability to capture the extreme events, based on recent designated dry year and wet year. All four datasets represent the annual and monthly climatology of Sri Lanka well; however, discrepancies are seen at seasonal scale. CHIRPS had the best performance in the wet zone and ERA5 in dry and intermediate zones. Although all datasets distinctly identified the wet year and dry year with respect to the normal average, the interannual variability was explicitly identified by CHIRPS and ERA5. Therefore, for replicating the national and zonal climatology, both ERA5 (dry and intermediate zones) and CHIRPS (wet zone) are suitable. With the perspective of hydrological applications, both datasets can be used in development of hydrological models for applications such as flood forecasting, reservoir management and climate change impact assessment as an alternative to observed rainfall data in data scarce regions.
Twenty-Five Years of Domoic Acid Monitoring in Galicia (NW Spain): Spatial, Temporal, and Interspecific Variations

Prevalence, impact on shellfish resources and interspecific, spatial, and temporal variabilities of domoic acid (DA) in bivalves from Galicia (NW Spain) have been studied based on more than 25 years of monitoring data. The maximum prevalence (samples in which DA was detected) (100%) and incidence (samples with DA levels above the regulatory limit) (97.4%) were recorded in Pecten maximus, and the minimum ones in Mytilus galloprovincialis (12.6 and 1.1%, respectively). The maximum DA concentrations were 663.9 mg kg−1 in P. maximus and 316 mg kg−1 in Venerupis corrugata. After excluding scallop P. maximus data, DA was found (prevalence) in 13.3% of bivalve samples, with 1.3% being over the regulatory limit. In general, the prevalence of this toxin decreased towards the North but not the magnitude of its episodes. The seasonal distribution was characterized by two maxima, in spring and autumn, with the later decreasing in intensity towards the north. DA levels decreased slightly over the studied period, although this decreasing trend was not linear. A cyclic pattern was observed in the interannual variability, with cycles of 4 and 11 years. Intoxication and detoxification rates were slower than those expected from laboratory experiments, suggesting the supply of DA during these phases plays an important role.
Finnish e-health services intended for citizens – national and regional development

Electronic health care (e-health) services intended for Finnish citizens have been recently developed nationally, regionally, and locally through several projects and programs. This study aimed to investigate the development and availability of e-health services for Finnish citizens in specialized and primary health care and private medical service providers from 2011 to 2020. In addition, the differences between the availability of services in different sectors and regional differences between hospital districts were investigated. Data were collected using web-based questionnaires in 2011, 2014, 2017, and 2020 from “Use of information and communication technology surveys in Finnish health care”. This study covers all 21 hospital districts, nearly all primary health care centers, and a sample of private medical service providers. Quantitative data were analyzed using SPSS software (version 25). The availability of an e-health service was calculated as a percentage of all respondents in each sector. The results of this study show that public and private health care organizations extensively offered health care services on their websites. Almost all organizations had information on well-being, provided services, contact methods, and locations, as well as options to send electronic feedback. Remote consultation, electronic appointment booking services, and telephone counseling were also extensively offered. This study revealed that the volume of e-health services increased from 2011 to 2020. For instance, remote consultation services and information exchange through encrypted email increased rapidly during follow-up periods in all service sectors. Comparing service sectors revealed that specialized health care covers e-health services more extensively than do primary health care and private service providers. According to this study, there are also clear differences in the availability of services between hospital districts and no hospital district offered all studied services. These results suggest the need to clarify national and regional development responsibilities and standardize the availability of e-health services within and between hospital districts.
Applying the Theory of Planned Behavior to Healthy Eating Intention among Malaysian Adults

Despite the rising awareness on practicing healthy eating habits, statistics show the severity of the health state of Malaysian population. This indicates that most people may not have transformed their awareness into healthier eating behaviors. In response to this discrepancy between awareness and behavior, it is crucial to develop some basis for understanding the intention towards healthy eating as intention is believed to lead to performing desired behaviors. Therefore, this study aimed to identify the significant factors influencing Malaysians’ healthy eating intention with the application of Theory of Planned Behavior (TPB). A sample of 1,719 adults participated in this study. The data were analyzed using multiple regression analysis. All exogenous con structs consisting of attitude (β = 0.570), perceived behavioral control (β = 0.244), and subjective norm (β = 0.082) have positive and significant relationship with healthy eating intention. The model explained 56.7% of the variance in healthy eating intention with attitude being the most influential predictor. The findings confirm the applicability of TPB and provide indispensable insights in understanding the factors influencing the healthy eating intention among Malaysian adults. These findings can be used further to develop effective intervention programs in promoting healthier eating behaviors among the population.
Effect of Enterococcus faecium AL41 (CCM8558) and Its Enterocin M on the Physicochemical Properties and Mineral Content of Rabbit Meat

Improving rabbit meat quality using natural substances has become an area of research activity in rabbit nutrition due to stabilization of husbandry health and economy. The present study evaluates the effect of bacteriocin-producing, beneficial strain Enterococcus faecium AL41/CCM8558 and its enterocin M (EntM) on the quality and mineral content of rabbit meat. Seventy-two Hycole rabbits (aged 35 days) were divided into EG1 (CCM8558 strain; 1.0 × 109 CFU/mL; 500 µL/animal/d), EG2 (EntM; 50 µL/animal/d), and control group (CG). The additives were administrated in drinking water for 21 days. Significant increase in meat phosphorus (EG1: p < 0.05; EG2: p < 0.0001) and iron (EG1, EG2: p < 0.001) contents was noted; sodium and zinc levels were only slightly higher in experimental groups compared with control data. The calcium (EG1, EG2: p < 0.001), potassium, and copper (EG1: p < 0.01) concentrations were reduced. The treatment did not have a negative influence on physicochemical traits of rabbit meat. Based on these results, we conclude that diet supplementation with beneficial strain E. faecium CCM8558 and its EntM could enhance the quality and mineral content of rabbit meat, with the focus on its iron and phosphorus contents.
Development methodologies for ontology‐based knowledge management systems: A review

Knowledge management systems (KMS) are computer‐based systems highly valued in business organizations because they support knowledge management (KM) processes. Most KMS have been developed using non‐intelligent computer technology—that is, DMS, CMS, DBMS, and CIS—, and thus, they cannot provide advanced capabilities. Consequently, enhanced KMS using intelligent technologies of ontologies with inference engines—called ontology‐based knowledge management systems (OKMS)—have been proposed in the last three decades. Nowadays, however, the implementation of OKMS in real‐world settings is still scarce. Lack of comprehensive and systematic development methodologies including Project Management and Technical Systems Engineering processes—as the Systems and Software Systems Engineering standards propose—have been suggested as a factor that inhibits OKMS implementations. In this study, we review the OKMS literature (1990–2021 period)—from six seminal studies located using a research search engine—to assess OKMS development methodologies that can be considered comprehensive and systematic. Five methodologies were identified and assessed using an evaluation subset from the ISO/IEC/IEEE 15288:2015 Systems and Software Engineering standard. Two of them—CommonKADS and NeON—were found with a high comprehensive and systematic level and both are suggested for organizations interested in OKMS implementations, but none of them qualified as agile, which is a current development approach for systems and software systems. Hence, further empirical research toward the realization of comprehensive and systematic OKMSs development methodologies, including agile versions, is suggested for fostering the implementation of OKMS in real‐world settings.
Facial Expression Recognition With Visual Transformers and Attentional Selective Fusion

Facial Expression Recognition (FER) in the wild is extremely challenging due to occlusions, variant head poses, face deformation and motion blur under unconstrained conditions. Although substantial progresses have been made in automatic FER in the past few decades, previous studies were mainly designed for lab-controlled FER. Real-world occlusions, variant head poses and other issues definitely increase the difficulty of FER on account of these information-deficient regions and complex backgrounds. Different from previous pure CNNs based methods, we argue that it is feasible and practical to translate facial images into sequences of visual words and perform expression recognition from a global perspective. Therefore, we propose the Visual Transformers with Feature Fusion (VTFF) to tackle FER in the wild by two main steps. First, we propose the attentional selective fusion (ASF) for leveraging two kinds of feature maps generated by two-branch CNNs. The ASF captures discriminative information by fusing multiple features with the global-local attention. The fused feature maps are then flattened and projected into sequences of visual words. Second, inspired by the success of Transformers in natural language processing, we propose to model relationships between these visual words with the global self-attention. The proposed method is evaluated on three public in-the-wild facial expression datasets (RAF-DB, FERPlus and AffectNet). Under the same settings, extensive experiments demonstrate that our method shows superior performance over other methods, setting new state of the art on RAF-DB with 88.14%, FERPlus with 88.81% and AffectNet with 61.85%. The cross-dataset evaluation on CK+ shows the promising generalization capability of the proposed method.
Association between Milk Intake and Incident Stroke among Japanese Community Dwellers: The Iwate-KENCO Study

We aimed to evaluate the association between the milk consumption and incident stroke in a Japanese population, where milk consumption is lower than that of Western countries. In total, 14,121 participants (4253 men and 9868 women) aged 40–69 years, free from cardiovascular diseases (CVD) were prospectively followed for 10.7 years. Participants were categorized into four groups according to the milk intake frequency obtained from a brief-type self-administered diet questionnaire. The adjusted HRs of total stroke, ischemic stroke and haemorrhagic stroke associated with milk intake frequency were calculated using the Cox proportional hazards model. During the follow-up, 478 stroke cases were detected (208 men and 270 women). Compared to women with a milk intake of <2 cups/week, those with an intake of 7 to <12 cups/week had a significantly low risk of ischemic stroke in a model adjusting CVD risk factors; the HR (95% CI) was 0.53 (0.32–0.88). No significant associations were found in men. This study suggested that milk intake of 7 to <12 cups/week decreased the risk of ischemic stroke in Japanese women. Milk intake of about 1 to <2 cups/day may be effective in the primary prevention of ischemic stroke in a population with low milk intake.
The Initial Human Atherosclerotic Lesion and Lipoprotein Modification—A Deep Connection

Atherosclerosis research typically focuses on the evolution of intermediate or advanced atherosclerotic lesions rather than on prelesional stages of atherogenesis. Yet these early events may provide decisive leads on the triggers of the pathologic process, before lesions become clinically overt. Thereby, it is mandatory to consider extracellular lipoprotein deposition at this stage as the prerequisite of foam cell formation leading to a remarkable accumulation of LDL (Low Density Lipoproteins). As progression of atherosclerosis displays the characteristic features of a chronic inflammatory process on the one hand and native LDL lacks inflammatory properties on the other hand, the lipoprotein must undergo biochemical modification to become atherogenic. During the last 25 years, evidence was accumulated in support of a different concept on atherogenesis proposing that modification of native LDL occurs through the action of ubiquitous hydrolytic enzymes (enzymatically modified LDL or eLDL) rather than oxidation and contending that the physiological events leading to macrophage uptake and reverse transport of eLDL first occur without inflammation (initiation with reversion). Preventing or reversing initial atherosclerotic lesions would avoid the later stages and therefore prevent clinical manifestations. This concept is in accordance with the response to retention hypothesis directly supporting the strategy of lowering plasma levels of atherogenic lipoproteins as the most successful therapy for atherosclerosis and its sequelae. Apart from but unquestionable closely related to this concept, there are several other hypotheses on atherosclerotic lesion initiation favoring an initiating role of the immune system (‘vascular-associated lymphoid tissue’ (VALT)), defining foam cell formation as a variant of lysosomal storage disease, relating to the concept of the inflammasome with crystalline cholesterol and/or mitochondrial DAMPs (damage-associated molecular patterns) being mandatory in driving arterial inflammation and, last but not least, pointing to miRNAs (micro RNAs) as pivotal players. However, direct anti-inflammatory therapies may prove successful as adjuvant components but will likely never be used in the absence of strategies to lower plasma levels of atherogenic lipoproteins, the key point of the perception that atherosclerosis is not simply an inevitable result of senescence. In particular, given the importance of chemical modifications for lipoprotein atherogenicity, regulation of the enzymes involved might be a tempting target for pharmacological research.