Miracle-dz/UniFAR-FTUs huggingface dataset:
CaRE @ Home: Pilot Study of an Online Multidimensional Cancer Rehabilitation and Exercise Program for Cancer Survivors

Background: Although facility-based cancer rehabilitation and exercise programs exist, patients are often unable to attend due to distance, cost, and other competing obligations. There is a need for scalable remote interventions that can reach and serve a larger population. Methods: We conducted a mixed methods pilot study to assess the feasibility, acceptability and impact of CaRE@Home: an 8-week online multidimensional cancer rehabilitation and exercise program. Feasibility and acceptability data were captured by attendance and adherence metrics and through qualitative interviews. Preliminary estimates of the effects of CaRE@Home on patient-reported and physically measured outcomes were calculated. Results: A total of n = 35 participated in the study. Recruitment (64%), retention (83%), and adherence (80%) rates, along with qualitative findings, support the feasibility of the CaRE@Home intervention. Acceptability was also high, and participants provided useful feedback for program improvements. Disability (WHODAS 2.0) scores significantly decreased from baseline (T1) to immediately post-intervention (T2) and three months post-intervention (T3) (p = 0.03 and p = 0.008). Physical activity (GSLTPAQ) levels significantly increased for both Total LSI (p = 0.007 and p = 0.0002) and moderate to strenuous LSI (p = 0.003 and p = 0.002) from baseline to T2 and T3. Work productivity (iPCQ) increased from T1 to T3 (p = 0.026). There was a significant increase in six minute walk distance from baseline to T2 and T3 (p < 0.001 and p = 0.010) and in grip strength from baseline to T2 and T3 (p = 0.003 and p < 0.001). Conclusions: Results indicate that the CaRE@Home program is a feasible and acceptable cancer rehabilitation program that may help cancer survivors regain functional ability and decrease disability. In order to confirm these findings, a controlled trial is required.
Long-term cocaine use is associated with increased coronary plaque burden – a pilot study

ABSTRACT Background: There is a lack of research regarding whether prolonged use of cocaine would lead to increase of coronary plaque burden. Objectives: To study the effects of cocaine use on the coronary artery plaque volume. We hypothesize the longer the cocaine use, the greater the plaque burden. Methods: We used coronary computed tomography angiography to evaluate plaque volumes. The study included chronic (N = 33 with 27 HIV+) and non-cocaine users (N = 15 with 12 HIV+). Chronic cocaine use was defined as use by any route for at least 6 months, administered at least 4 times/month. The Student’s t-test was used to compare the plaque volumes between chronic and non-cocaine users. Multivariable regression analysis adjusted for age, sex, body mass index, HIV status, cigarette smoking, diabetes, and total cholesterol was performed to determine the relationship between years of cocaine use and plaque volumes. Results: The total plaque volumes between groups showed no difference (p = .065). However, the total left anterior descending artery (LAD) plaque volume in the chronic cocaine group was significantly higher than that in the non-cocaine group (p = .047). For each year increase in cocaine use, total plaque volume and total LAD plaque volume increased by 7.23 mm3 (p = .013) and 4.56 mm3 (p = .001), respectively. In the multivariable analyses, both total plaque volume and total LAD plaque volume were significantly associated with years of cocaine use (p = .039 and 0.013, respectively). Conclusion: Prolonged cocaine use accelerates the development of sub-clinical atherosclerosis.
Mineral Contents in Edible Mushrooms

野生食用キノコ24種27点と栽培種5種6点について,K, Na, Ca, Mg, P, Fe, Cu, Zn, Mn, Cd, Pb, ASおよびHgの13種元素の含有量を測定した. これらのキノコは主に東京,東北地方,中部地方において,1979年および1980年に採取または購入したものである. 元素の含有量は乾燥重量当たりで以下にご示す範囲であった. K: 1.1～5.3%, Na: 11～267mg/100g, Ca: 5～342mg/100g, Mg: 59～299mg/100g, P: 59～1938mg/100g, Fe: 4.9～419.4mg/100g, Cu: 0.2～23.2mg/100g, Zn: 0.4～16.3mg/100g, Mn: 0.8～10.2mg/100g, Cd:0.0～38.8ppm, Pb: 0.0～17.1ppm, AS:0.0～18.0ppm, Hg: 0.0～6.3ppm. キノコの種の違いによって無機質含有量に著しい差がみられた. 同種間では無機質量にいくつかの特徴が認められた.
Autologous noncultured, nontrypsinized melanocyte‐keratinocyte graft homogenized in plasma gel followed by narrow‐band ultraviolet B therapy for stable vitiligo: A novel technique

There is a continuous need for modifications of epidermal cell grafting techniques treating stable vitiligo to make it easier, more economic, and with better outcome. To evaluate the efficacy and safety of noncultured, nontrypsinized epidermal cell graft homogenized with plasma gel, followed by narrow band‐ultraviolet B (NB‐UVB) in treatment of stable vitiligo, about 40 patients with stable vitiligo underwent harvesting of epidermal cells from the donor site by dermabrasion then the harvested cells were prepared, homogenized with autologous plasma gel, and applied to the abraded recipient, followed by 16 NB‐UVB sessions after complete healing. Patches within the same anatomical site received only NB‐UVB as controls. The percentage of improvement ranged from 23.33% to 100% with more than 75% uniform, homogenous repigmentation in 65% of patients. Donor site healed completely with normal skin. Noncultured, nontrypsinized epidermal cell grafting technique homogenized with plasma gel followed by NB‐UVB sessions gave positive responses and was well tolerated in different body sites in stable resistant vitiligo.
A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource Settings

Quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay is the gold standard recommended to test for acute SARS-CoV-2 infection. However, it generally requires expensive equipment such as RNA isolation instruments and real-time PCR thermal cyclers. As a pandemic, COVID-19 has spread indiscriminately, and many low resource settings and developing countries do not have the means for fast and accurate COVID-19 detection to control the outbreak. Additionally, long assay times, in part caused by slow sample preparation steps, have created a large backlog when testing patient samples suspected of COVID-19. With many PCR-based molecular assays including an extraction step, this can take a significant amount of time and labor, especially if the extraction is performed manually. Using COVID-19 clinical specimens, we have collected evidence that the RT-qPCR assay can feasibly be performed directly on patient sample material in virus transport medium (VTM) without an RNA extraction step, while still producing sensitive test results. If RNA extraction steps can be omitted without significantly affecting clinical sensitivity, the turn-around time of COVID-19 tests, and the backlog we currently experience can be reduced drastically. Furthermore, our data suggest that rapid RT-PCR can be implemented for sensitive and specific molecular diagnosis of COVID-19 in locations where sophisticated laboratory instruments are not available. Our USD 300 set up achieved rapid RT-PCR using thin-walled PCR tubes and a water bath setup using sous vide immersion heaters, a Raspberry Pi computer, and a single servo motor that can process up to 96 samples at a time. Using COVID-19 positive clinical specimens, we demonstrated that RT-PCR assays can be performed in as little as 12 min using untreated samples, heat-inactivated samples, or extracted RNA templates with our low-cost water bath setup. These findings can help rapid COVID-19 testing to become more accessible and attainable across the globe.
Species variation in water relations and xylem vulnerability to cavitation at a forest-woodland ecotone

Xylem vulnerability to cavitation and response of water potential (), stomatal conductance (g s), and net photosynthesis (P n) to drought are potentially important mechanisms of drought resistance. We compared , g s, Pn, and cavitation vulnerability of shoot and root xylem among co-occurring ponderosa pine (Pinus ponderosa var. scopulorum Dougl. Ex Laws.), pinyon pine (Pinus edulis Engelm.), and Utah juniper (Juniperus osteosperma (Torr.) Little) at a forest-woodland ecotonal site in northern Arizona to elucidate drought resistance mechanisms of these species. Juniper shoots partly regulated during drought via stomatal closure, but regulation was weaker than that for ponderosa and pinyon pines, which had similar water relations and P n responses to drought. Midday g s and Pn during summer drought were positive for juniper (gs 14.3 mmol m 2 s 1 , Pn 1.23 mol m 2 s 1 ) but near zero for ponderosa (gs 0.7 mmol m 2 s 1 , Pn 0.02 mol m 2 s 1 ) and pinyon (gs 1.5 mmol m 2 s 1 , Pn 0.18 mol m 2 s 1 ) pines. Cavitation vulnerability of shoots and roots was lower for juniper than for both pines. The water potential inducing 50% loss in xylem hydraulic conductivity ( 50) for juniper was 5.0 MPa more negative for shoots and 3.9 MPa more negative for roots compared with the respective tissues of the pine species. Pinyon pine ( 50 2.71 MPa) was slightly more vulnerable to cavitation than ponderosa pine ( 50 3.42 MPa) for shoots, whereas root vulnerability was similar for both pines ( 50 1.69 MPa for pinyon; 1.98 MPa for ponderosa). Roots of all species were more vulnerable to cavitation than shoots. Our results show an important role of cavitation vulnerability in the greater drought resistance of Utah juniper than pinyon and ponderosa pines but not for the presumed greater drought resistance of pinyon pine than ponderosa pine. FOR . S CI. 59(5):524-535.
Dynamics of endoreduplication in developing barley seeds.

Seeds are complex biological systems comprising three genetically distinct tissues: embryo, endosperm, and maternal tissues (including seed coats and pericarp) nested inside one another. Cereal grains represent a special type of seeds with the largest part formed by endosperm, a specialized triploid tissue ensuring embryo protection and nourishment. We investigated the dynamics of DNA amount changes of the three major seed tissues from the time of pollination until dry seed. We show that cell cycle is under strict developmental control in different seed compartments. After an initial wave of active cell division, cells switch to endocycle and most endoreduplication events are observed in endosperm and seed maternal tissues. Using different barley cultivars we show that there is natural variation in the kinetics of this process. During the terminal stages of seed development, specific and selective loss of endoreduplicated nuclei occurs in the endosperm. This is accompanied by reduced stability of nuclear genome, progressive loss of cell viability and finally programmed cell death. In summary, our study shows, that endopolyploidization and cell death are linked phenomena that frame the barley grain development.
Biodegradation of Total Petroleum Hydrocarbon by Aerobic Heterotrophic Bacteria Isolated from Crude Oil Contaminated Brackish Waters of Bodo Creek

Bodo creek is located in Gokana Local Government Area of Rivers state and is characterised by brackish water which is heavily contaminated with crude oil. Water samples collected from the creek were taken to Environmental Microbiology Laboratory of University of Portharcourt for isolation of aerobic heterotrophic bacteria using serial dilution spread plate technique. Isolation of morphologically distinct pure cultures was done and the isolates obtained were identified molecularly on the basis of 16S rRNA gene sequence analysis. 16S rRNA sequencing for identification of the isolates generated sequences ranging from 500 bp to 1700 bp and a 250 bp size PCR amplified fragment. The nucleotide sequence of the 16S rRNA gene was compared with published 16S rRNA sequences using BLAST search at the data base of National Centre for Biotechnology Information (NCBI). Biodegradation of petroleum hydrocarbon test on the isolates was conducted using Bony light crude oil obtained from Shell Petroleum Development Company and monitored using GC-FID (Agilent 6890 model) for 49 days. The initial quantity of TPH was 24091 and 6706 mg/l on day 0 for aerobic heterotrophic treatment and crude oil contaminated control and decreased progressively to 212.8 and 1174 mg/l respectively on day 49 indicating biodegradation in the treatment and control. Loss of TPH was statistically significant using one way analysis of variance (ANOVA) p<0.05 with time. Model for predicting TPH loss was developed and rate of biodegradation of the isolates determined using Trend line method of Microsoft Excel, 2010. The growth of bacteria cells increased progressively with decrease in TPH implying that the bacteria were responsible for biodegradation. Further application of bioremediation strategies in Bodo creek for biostimulation of the crude oil biodegrading bacteria for reclamation and restoration of an efficient ecosystem structure and function is suggested.
Retinal defocus and form‐deprivation induced regional differential gene expression of bone morphogenetic proteins in chick retinal pigment epithelium

We previously reported bidirectional gene expression regulation of the Bone Morphogenetic Proteins (BMP2, 4, and 7) in chick retinal pigment epithelium (RPE) in response to imposed optical defocus and form‐deprivation (FD). This study investigated whether there are local (regional) differences in these effects. 19‐day old White‐Leghorn chicks wore monocular +10 or − 10 D lenses, or diffusers (FD) for 2 or 48 hr, after which RPE samples were collected from both eyes, from a central circular zone (3 mm radius), and 3 mm wide annular mid‐peripheral and peripheral zones in all cases. BMP2, 4, and 7 gene expression levels in RPE from treated and fellow control eyes were compared as well as differences across zones. With the +10 D lens, increased expression of both BMP2 and BMP4 genes was observed in central and mid‐peripheral zones but not the peripheral zone after 2 and 48 hr. In contrast, with the −10 D lens BMP2 gene expression was significantly decreased in all three zones after 2 and 48 hr. Similar patterns of BMP2 gene expression were observed in all three zones after 48 hr of FD. Smaller changes were recorded for BMP4 and BMP7 gene expression for both myopia‐inducing treatments. That optical defocus‐ and FD‐induced changes in BMP gene expression in chick RPE show treatment‐dependent local (regional) differences suggest important differences in the nature and contributions of local retinal and underlying RPE regions to eye growth regulation.
Cetaceans and tuna purse seine fisheries in the Atlantic and Indian Oceans: interactions but few mortalities

Fisheries bycatch is considered to be one of the most significant causes of mortality for many marine species, including vulnerable megafauna. In the open ocean, tuna purse seiners are known to use several cetacean species to detect tuna schools. This exposes the cetaceans to encirclement which can lead to incidental injury or death. While interactions between fishers and cetaceans have been well documented in the eastern tropical Pacific Ocean, little is known about these interactions and potential mortalities in the tropical Atlantic and Indian Oceans. Here, we provide the first quantification of these interactions in both oceans by analyzing a large database of captain's logbooks (1980 to 2011) and observations collected by onboard scientific observers (1995 to 2011). Distribution maps of sightings per unit effort highlighted main areas of relatively high co-occurrence: east of the Seychelles (December to March), the Mozambique Channel (April to May) and the offshore waters of Gabon (April to September). The percentage of cetacean- associated fishing sets was around 3% in both oceans and datasets whereas 0.6% of sets had cetaceans encircled. Of the 194 cetaceans encircled in a purse seine net (122 baleen whales, 72 delphinids), immediate apparent survival rates were high (Atlantic: 92%, Indian: 100%). Among recorded mortalities, 8 involved pantropical spotted dolphins Stenella attenuata and 3 involved humpback whales Megaptera novaeangliae. These high survival rates suggest that setting nets close to cetaceans has a low immediate apparent impact on the species involved. Our findings will contribute to the development of an ecosystem approach to managing fisheries and accurate cetacean conservation measures.
Effects of a Workplace Violence Intervention on Hospital Employee Perceptions of Organizational Safety.

OBJECTIVE To examine hospital employee perceptions of organizational safety one-year following a workplace violence intervention and to evaluate perceptions based on violence exposure status. METHODS In 2014, 343 employees across 41 hospital units (N = 21 control units, N = 20 intervention units) completed a questionnaire capturing organizational safety perceptions and violence exposure. RESULTS Intervention unit employees reported more positive perceptions of organizational safety compared to control unit employees. However, intervention group employees who experienced patient-to-worker violence (Type II) had significantly more positive perceptions than those who experienced worker-to-worker violence (Type III). CONCLUSIONS Organizational safety perceptions improved following a violence-prevention intervention, especially among employees who reported Type II violence. Certain employees report worsened safety perceptions. Hospitals and units that implement violence prevention interventions should strive to address all types of violent behavior.
Effects of gp120 Inner Domain (ID2) Immunogen Doses on Elicitation of Anti-HIV-1 Functional Fc-Effector Response to C1/C2 (Cluster A) Epitopes in Mice

Fc-mediated effector functions of antibodies, including antibody-dependent cytotoxicity (ADCC), have been shown to contribute to vaccine-induced protection from HIV-1 infection, especially those directed against non-neutralizing, CD4 inducible (CD4i) epitopes within the gp120 constant 1 and 2 regions (C1/C2 or Cluster A epitopes). However, recent passive immunization studies have not been able to definitively confirm roles for these antibodies in HIV-1 prevention mostly due to the complications of cross-species Fc–FcR interactions and suboptimal dosing strategies. Here, we use our stabilized gp120 Inner domain (ID2) immunogen that displays the Cluster A epitopes within a minimal structural unit of HIV-1 Env to investigate an immunization protocol that induces a fine-tuned antibody repertoire capable of an effective Fc-effector response. This includes the generation of isotypes and the enhanced antibody specificity known to be vital for maximal Fc-effector activities, while minimizing the induction of isotypes know to be detrimental for these functions. Although our studies were done in in BALB/c mice we conclude that when optimally titrated for the species of interest, ID2 with GLA-SE adjuvant will elicit high titers of antibodies targeting the Cluster A region with potent Fc-mediated effector functions, making it a valuable immunogen candidate for testing an exclusive role of non-neutralizing antibody response in HIV-1 protection in vaccine settings.
Mindfulness‐based arts interventions for cancer care: A systematic review of the effects on wellbeing and fatigue

Upon receiving a cancer diagnosis, life irrevocably changes and complex experiences of emotional distress often occur. There is a growing interest in mindfulness‐based arts interventions (MBAIs) to ameliorate the distress many patients experience. Our review objective was to synthesize the evidence on the effectiveness of MBAIs on psychological wellbeing and fatigue.
Three-Dimensional Culture System of Cancer Cells Combined with Biomaterials for Drug Screening

Simple Summary For the research and development of drug discovery, it is of prime importance to construct the three-dimensional (3D) tissue models in vitro. To this end, the enhancement design of cell function and activity by making use of biomaterials is essential. In this review, 3D culture systems of cancer cells combined with several biomaterials for anticancer drug screening are introduced. Abstract Anticancer drug screening is one of the most important research and development processes to develop new drugs for cancer treatment. However, there is a problem resulting in gaps between the in vitro drug screening and preclinical or clinical study. This is mainly because the condition of cancer cell culture is quite different from that in vivo. As a trial to mimic the in vivo cancer environment, there has been some research on a three-dimensional (3D) culture system by making use of biomaterials. The 3D culture technologies enable us to give cancer cells an in vitro environment close to the in vivo condition. Cancer cells modified to replicate the in vivo cancer environment will promote the biological research or drug discovery of cancers. This review introduces the in vitro research of 3D cell culture systems with biomaterials in addition to a brief summary of the cancer environment.
Efficacy and Safety of Anti-Interleukin-17A Monoclonal Antibody Secukinumab in Treatment of Ankylosing Spondylitis: A Meta-Analysis.

Different signaling pathways have been studied in ankylosing spondylitis. New treatment options such as secukinumab could have an important role inhibiting the release of proinflammatory cytokine IL-17. The aim of this study was to compare the efficacy and safety of secukinumab in ankylosing spondylitis. A systematic review was conducted using MEDLINE and EMBASE databases to identify randomized clinical trials (RCTs) that assess the role of secukinumab in ankylosing spondylitis. The variables were safety (total adverse events, serious adverse events, headache, nasopharyngitis, cough, deaths, discontinuation due to adverse events, candida, neutropenia, and diarrhea) and efficacy based on quality-of-life scores (ASAS 20, ASAS 40, ASAS 5/6, ASASPR). Three RCTs (770 patients) that compare secukinumab with placebo were included in the study. There were significant differences in the quality-of-life scores in favor of the secukinumab group (p < 0.05). Regarding the adverse events, there were higher rates of any adverse events in the secukinumab group (p < 0.05). Also, the secukinumab group showed a higher rate of nasopharyngitis and diarrhea (p < 0.05). The use of secukinumab in ankylosing spondylitis increased the quality of life and had more adverse events rate compared with placebo.
Moisture availability mediates the relationship between terrestrial gross primary production and solar‐induced chlorophyll fluorescence: Insights from global‐scale variations

Effective use of solar‐induced chlorophyll fluorescence (SIF) to estimate and monitor gross primary production (GPP) in terrestrial ecosystems requires a comprehensive understanding and quantification of the relationship between SIF and GPP. To date, this understanding is incomplete and somewhat controversial in the literature. Here we derived the GPP/SIF ratio from multiple data sources as a diagnostic metric to explore its global‐scale patterns of spatial variation and potential climatic dependence. We found that the growing season GPP/SIF ratio varied substantially across global land surfaces, with the highest ratios consistently found in boreal regions. Spatial variation in GPP/SIF was strongly modulated by climate variables. The most striking pattern was a consistent decrease in GPP/SIF from cold‐and‐wet climates to hot‐and‐dry climates. We propose that the reduction in GPP/SIF with decreasing moisture availability may be related to stomatal responses to aridity. Furthermore, we show that GPP/SIF can be empirically modeled from climate variables using a machine learning (random forest) framework, which can improve the modeling of ecosystem production and quantify its uncertainty in global terrestrial biosphere models. Our results point to the need for targeted field and experimental studies to better understand the patterns observed and to improve the modeling of the relationship between SIF and GPP over broad scales.