Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of collagen gene expression by transformed human fibroblasts : decreased type I and type III collagen RNA transcription

The regulation of collagen gene expression in normal diploid human fetal fibroblasts (KMS-6 cells), and fibroblasts immortally transformed by treatment of KMS-6 with Co-60 gamma rays (KMST-6 cells) was compared to that of ones tumorigenically transformed by treatment of KMST-6 cells with Harvey murine sarcoma virus (KMST-6-Ras cells). Synthesized collagenous protein decreased to approximately 30% of that of normal fetal fibroblasts in both transformed cell lines, and the relative rate of collagen synthesis to total protein synthesis decreased about sixfold in KMST-6 cells and twelvefold in KMST-6-Ras cells. The m-RNA levels of type I collagen in both of these cell lines decreased to approximately 20% of that of the control fibroblasts, whereas type III collagen m-RNA levels decreased to only 9% of that of the control. The copy number of the collagen gene in both transformed cell lines was unaltered. The transcriptional rates of collagen alpha 1(I) and collagen alpha 1(III) in both cell lines decreased to 20% and 7% respectively of that of control. These data indicate that collagen synthesis was reduced at the transcriptional level in these transformed human fibroblasts.
Genotype by environment interaction for carcass traits and intramuscular fat content in heavy Iberian pigs fattened in two different free-range systems

Genotype by environment interaction (G×E) is a potential source of reduced efficiency in genetic improvement programs in livestock. The objective of the current work consisted of checking the existence of G×E interaction in carcass traits and in intramuscular fat content (IMF) in Iberian pigs fattened in two free-range systems. Genetic component and estimated breeding values (EBV) of the percentage of hams, shoulders and loins and IMF in loin were obtained from records of 4,348 and 1,818 pigs fattened in campo (C) and montanera (M) systems, respectively. A multitrait model where the performances of each system are considered as different traits was implemented. Three selection indexes were built with different treatments about the quality trait, two of them based in the optimal trait theory. The Pearson correlation between EBV and indexes and the Spearman correlation between the rankings of progenies of 21 boars fattened in both systems were calculated. Heritability results were different in both systems (h2 range from 0.43 to 0.66 and from 0.24 to 0.33 in C and M system, respectively) and genetic correlation of same traits expressed in the two systems also pointed out to a weak G×E interaction (0.64, 0.67 and 0.66 in hams, shoulders and IMF, respectively). Pearson and Spearman correlations were always significantly different to 1. The obtained results advised to consider this G×E interaction in the analysis model of a breeding program focused on free range production system and to include IMF in the index selection assuming an optimum range for this quality trait, in order to avoid negative effects of selection for carcass performances.
Magic and Politics

This essay introduces a special issue of Nova Religio on magic and politics in the United States in the aftermath of the 2016 presidential election. The articles in this issue address a gap in the literature examining intersections of religion, magic, and politics in contemporary North America. They approach political magic as an essentially religious phenomenon, in that it deals with the spirit world and attempts to motivate human behavior through the use of symbols. Covering a range of practices from the far right to the far left, the articles argue against prevailing scholarly treatments of the use of esoteric technologies as a predominantly right-wing phenomenon, showing how they have also been operationalized by the left in recent history. They showcase the creativity of magic as a form of human cultural expression, and demonstrate how magic coexists with rationality in contemporary western settings.
MICROBIOLOGICAL STUDY OF ARZÚA CHEESE (NW SPAIN) THROUGHOUT CHEESEMAKING AND RIPENING

Changes during production and ripening in the microbial flora of 11 batches of Arzua, a soft cheese made from raw cow's milk, were investigated. The following microbial groups were counted on the surface and interior of the cheese: total viable count (TVC), lactic acid bacteria (LAB), halotolerant flora, enterococci, proteolytic enterococci, staphylococci, Staphylococcus aureus, Enterobacteriaceae, faecal coliforms, molds, yeasts, Listeria spp. and (in milk) Brucella spp. pH and water activity were also determined. TVC and LAB were, generally, more than 9 log (cfu/g). Enterococci counts increased gradually, reaching values in excess of 6 log units. Halotolerant flora and staphylococci remained practically constant throughout ripening, at 6–8 and 5–7 log units, respectively. Maximum Enterobacteriaceae and faecal coliform counts exceeded 7 and 6 log units, respectively. Brucella spp. were not detected in any of the milk samples. Listeria spp. were detected in four batches, and Listeria monocytogenes in two.
Using a Brain Based Learning Program for Improving Preparatory Stage EFL Students' Vocabulary Retention

The study attempted to investigate the effect of using a brain based learning program for improving preparatory stage EFL students' vocabulary retention. Sixty students from El-Shaheed Ahmad Ibrahiem preparatory school, Minia Educational Directorate, Minia governorate were randomly selected. The study adopted the quasi experimental research design as sixty participants were divided into two groups. The treatment group (n= 30) was taught by using the brain based learning program (BBLP), while the non-treatment group (n=30) was using the regular way of instruction. A vocabulary retention test was developed by the researcher and used in the study. Analysis of the collected data indicated that the participants of the treatment group significantly outperformed the non-treatment one in the post-performance of the vocabulary retention. It was concluded that the brain based learning program helped first year preparatory school EFL students to improve their vocabulary retention.
The Action of Ipecacuanha Alkaloids on Entamoeba histolytica and some other Entozoic Amoebae in Culture

It is now recognized, by all competent observers, that emetine is a specific therapeutic agent for the treatment of amoebic dysentery; but although it is known that a thorough course of emetine, in some form, will eradicate an infection with Entamoeba histolytica from most human beings, there is still complete ignorance as to how the cure is effected. The obvious hypothesis, that emetine is a specific poison for E. histolytica, has not hitherto been supported by adequate experimental evidence. It is true that observers have described experiments in which the alkaloid, in high dilution, has apparently killed various amoebae in vitro; but these experiments either ignored the peculiarly specific action of emetine in vivo on E. histolytica, or were carried out without adequate controls.
Glucose antimetabolite 2-Deoxy-D-Glucose and its derivative as promising candidates for tackling COVID-19: Insights derived from in silico docking and molecular simulations

A novel respiratory pathogen, SARS-CoV-2 has recently received worldwide attention and has been declared a public health emergency of global concern. Entry of SARS-CoV-2 is mediated through the viral spike glycoprotein (S2). Afterwards, the virus gets hold of the host cell machinery by employing the use of viral main protease 3CLpro and NSP15 endoribonuclease. In the present in silico study, active site mapping of the viral virulence factors was rendered by means of DoG Site Scorer. The possibility of repurposing of 2-deoxy-D-glucose (2-DG), a radio-chemo-modifier drug used for optimizing cancer therapy, and one of its derivative (1, 3, 4, 6-Tetra-O-acetyl-2-deoxy-D-glucopyranose, has been investigated by conducting ligand-receptor docking. Binding pose depictions of ligands and viral receptors were assessed by employing molecular dynamics analysis. Molinspiration and Toxicity Estimation Software tools were used to assess the drug likeliness, bioactivity indices and ADMETox values. 2-DG can dock efficiently with viral main protease 3CLpro as well as NSP15 endoribonuclease, thus efficiently inactivating these viral receptors leading to incapacitation of the SARS-CoV-2 virus. Such incapacitation was possible by means of formation of a hydrogen bond between 2-DG and proline residues of viral protease. The 2-DG derivative formed a hydrogen bond with the glutamine amino acid residues of the viral spike glycoprotein. The present in silico study supports the potential benefits of using 2-DG and its glucopyranose derivative as repurposed drugs/prodrugs for mitigating the novel COVID-19 infection. Since both these moieties present no signs of serious toxicity, further empirical studies on model systems and human clinical trials to ascertain effective dose-response are warranted and should be urgently initiated.
Functional Properties of Guar Seed (Cyamopsis tetragonoloba) Meal Detoxified by Different Methods

Functional properties of guar meal detoxified by autoclaving, extraction with aqueous ethanol, methanol, isopropanol or dilute hydrochloric acid were compared with those of defatted soy meal. Acid washed and the autoclaved meals gave low nitrogen solubility (NS) at pH 7.0. Detoxified guar meals had greater water absorption capacity (WAC) than the raw meal, while defatted soy meal was far superior to defatted guar meal. Fat emulsification capacity (EC) showed a similar trend as NS and fat absorption capacity (FAC) inversely correlated with the bulk density of samples. Autoclaved guar meal had higher EC and FAC than the alcohol or acid extracted meals. The foam capacity (FC) and foam stability (FS) of the 80% isopropanol extracted guar meal were the highest among the samples.
Texture Analysis of Apparent Diffusion Coefficient Maps in Cervical Carcinoma: Correlation with Histopathologic Findings and Prognosis.

Purpose To determine the feasibility of texture analysis of apparent diffusion coefficient (ADC) maps and to assess the performance of texture analysis and ADC to predict histologic grade, parametrial invasion, lymph node metastasis, International Federation of Gynecology and Obstetrics (FIGO) stage, recurrence, and recurrence-free survival (RFS) in patients with cervical carcinoma. Materials and Methods This retrospective study included 58 patients with cervical carcinoma who were examined with a 1.5-T MRI system and diffusion-weighted imaging with b values of 0 and 1000 sec/mm2. Software with volumes of interest on ADC maps was used to extract 45 texture features, including higher-order texture features. Receiver operating characteristic (ROC) analysis was performed to compare the diagnostic performance of ADC map random forest models and of ADC values. Dunnett test, Spearman rank correlation coefficient, Kaplan-Meier analyses, log-rank test, and Cox proportional hazards regression analyses were also used for statistical analyses. Results The ADC map random forest models showed a significantly larger area under the ROC curve (AUC) than the AUC of ADC values for predicting high-grade cervical carcinoma (P = .0036), but not for parametrial invasion, lymph node metastasis, stages III-IV, and recurrence (P = .0602, .3176, .0924, and .5633, respectively). The random forest models predicted that the mean RFS rates were significantly shorter for high-grade cervical carcinomas, parametrial invasion, lymph node metastasis, stages III-IV, and recurrence (P = .0405, < .0001, .0344, .0001, and .0015, respectively); the random forest models for parametrial invasion and stages III-IV were more useful than ADC values (P = .0018) for predicting RFS. Conclusion The ADC map random forest models were more useful for noninvasively evaluating histologic grade, parametrial invasion, lymph node metastasis, FIGO stage, and recurrence and for predicting RFS in patients with cervical carcinoma than were ADC values.Keywords: Comparative Studies, Genital/Reproductive, MR-Diffusion Weighted Imaging, MR-Imaging, Neoplasms-Primary, Pathology, Pelvis, Tissue Characterization, UterusSupplemental material is available for this article. © RSNA, 2020See also the commentary by Reinhold and Nougaret in this issue.
Interacting effects of interrupted humid periods and light on infection of wheat leaves by Mycosphaerella graminicola (Septoria tritici)

Septoria tritici conidia require a long period at 100%, humidity to infect wheat plants successfully. When periods of 100% relative humidity were interrupted by up to 48 h at 75% relative humidity, infection by S. tritici on winter wheat cvs Longbow and Avalon was only slightly reduced. Breaks at 50% relative humidity had larger effects, but still allowed infection to occur. Infection was reduced more by a break in humidity which began in the light, but previous and subsequent light regimes strongly influenced the final outcome in all cases. When a wet period was interrupted twice, the two dry breaks interacted strongly under some circumstances; in these cases the probability of infection was much greater than expected from multiplying the effects of the single breaks. Under other conditions two breaks had more nearly multiplicative effects, or interacted to reduce the probability of infection below that expected from the individual breaks. There was evidence that light reduced the probability of successful infection earls in the infection process, but stimulated it later. The data were consistent with infection occurring faster on cv. Longbow, but otherwise being affected in qualitatively similar ways on both cultivars. The complexity of the effects and their interactions suggests that it will not be possible to find a simple set of infection conditions to apply in the field. The data rule out a number of simple mathematical models of the infection process.
The Physico-chemical and Sensory Characteristics of Kakdoogi Containing Hydrangea serrata Seringe Extract

To evaluate the effect of Hydrangea serrata Seringe extract on kakdoogi(Korean radish kimchi), the kakdoogi was prepared, after supplementation, with Hydrangea serrata Seringe extract(0, 1.25, 2.5 and 5%(v/w)). The quality characteristics of the kakdoogi were investigated during 7 days of fermentation at . The proximate components of the Hydrangea serrata Seringe leaves were 3.48% crude protein, 6.45% crude lipid, 7.98% crude fiber and 9.32% crude ash. The pH values in the samples with the extract were higher than that of both the control group and the sugar group. The total acidity levels in the sugar group was higher than that of other groups. The sugar group showed higher L value(lightness) than that of kakdoogi fermented with the extract. A value(redness) and B value(yellowness) of the supplemented kakdoogi was lower than that of the control and the sugar group on day 7. The hardness of samples with the extract was higher than that of the sugar group. And the hardness of the groups with the extract was not reduced during the fermentation period. The control group showed higher salinity than the other groups by day 4. The samples with the extract were sweeter than the control group on day 0 then the difference disappeared after day 2. Sourness of all the groups increased during the fermentation period. However the extract did not affect the hardness or overall acceptability of the kakdoogi during fermentation.
Dinophysis - a planktonic dinoflagellate genus which can act both as a prey and a predator of a ciliate

Heterotrophic members of the marine plankton dinoflagellate genus Dinophysis are specialized predators, whose food includes the prostomatid ciliate Tiarina fusus. This ciliate differs from most planktonic ciliates in its ability to ingest prey of its own size including autotrophic Dinophysis spp. However, when trying to catch a heterotrophic Dinophy- sis sp. , the ciliate is trapped instead by the dinoflagellate and emptied via a feeding tube (peduncle), which originates from the flagellar pore of the dinoflagellate. The specific predation on a ciliate by a heterotrophic dinoflagellate represents a new trophic link in the marine planktonic food web. The existence of colourl.ess thecate dinoflagellates in the marine pelagial has been recognized among taxo- nomists for more than a century (Gaines & Elbrachter 1987). In spite of this, few ecological studies dealing with the marine planktonic food webs have included them in the protozoan plankton even though they may make up a substantial part of the biomass of this group (Smetacek 1981). Knowledge about their trophic role in
Antiulcer Activity of Salvadora persica on Experimental ASA-Induced Ulcer in Rats: Ultrastructural Modifications

The lyophilized decoction of Salvadora persica L. roots possesses a significant protective effect on ulceration induced by ethanol, indomethacin and cold restraint stress in rats. In this work, we study the effect of chronic intragastric administration of S. persica decoction on experimental acetylsalicylic acid (ASA)-induced ulcer in rats. The ulcer index significantly decreased (U.I. 0.9 ± 1.6; P < 0.05) after treatment with a lyophilized decoction of S. persica (500 mg/kg, os), once daily for seven days, with respect to controls (U.I. 11.4 ± 2.3). The modification of gastric mucosa was observed by transmission electronic microscopy (TEM) confirming this result. In fact, in treated rats, the mucosa recovered to normal distribution. After S. persica treatment, some changes were detected in profiles of various cytoplasm organelles of parietal cells. Particularly, the intracellular canaliculi show an enlarged lumen with an increase in the number and length of microvilli. These morphological features of parietal cells after S. persica treatment suggest that the cells tend to recover a moderate secretory activity, even if the drug still discloses its inhibitory effect. Moreover, S. persica decoction possesses significant antiinflammatory activity.
Combining a regional climate model with a phytoplankton community model to predict future changes in phytoplankton in lakes

Summary 1. Linking a regional climate model (RCM) configured for contemporary atmospheric greenhouse gas concentrations, with a phytoplankton community model (PROTECH) produced realistic simulations of 20 years of recent phytoplankton data from Bassenthwaite Lake, in the North-West of England. 2. Meteorological drivers were derived from the RCM to represent a future climate scenario involving a 1% per annum compound increase in atmospheric CO2 concentrations until 2100. Using these drivers, PROTECH was run for another 20 year period representing the last two decades of the 21st century. 3. Comparison of these present and future simulations revealed likely impacts on the current seasonal phytoplankton development. Under future climate conditions, the simulated spring bloom showed an increase in cyanobacteria dominance caused by greater success of Planktothrix. Also, the summer cyanobacteria bloom declined earlier because of nutrient limitation caused by the increased spring growth. Overall productivity in the lake did not change. 4. Analysis showed that these predicted changes were driven by changes in water temperature, which were in turn triggered by the higher air temperatures predicted by the RCM.
Effect of Sulfur Dioxide Inhalation on Lung Microbiota in Rat Model

Purpose: To investigate the effect of sulfur dioxide on the lung microbiota of healthy rats. Methods Fifteen male rats were randomly divided into high dose and low dose exposure group and control group. After 7 days of SO2 exposure, the lung tissues were obtained and the lung microbiota was identified by Illumina high-throughput sequencing. Results The microbial community of lung microbiota was significantly alternated in the exposure group and the dominant phylum changed from Firmicutes to Proteobacteria. In addition, the SO2 exposure caused the bronchial wall thickening and a large number of inflammatory cell infiltration in the lungs of rats in exposure groups. Conclusions The results suggest that SO2 can significantly alter the lung microbiota and pathological structure of the lungs.
Decomposition of the herbicide EPTC in soils with a history of previous EPTC applications

Abstract The degradation of the herbicide EPTC (S-ethyl N, N-dipropylthiocarbamate) in 3 soils, with and without, previous field exposure to this herbicide was investigated. The influence of storage of soil (12 months) on the subsequent breakdown of EPTC was investigated. Soils with added EPTC were incubated (22°C), and the fate of EPTC was followed using gas liquid chromatogaphy. The herbicide degraded more rapidly in soils with 3 and 4 years of previous' exposure to annual applications of EPTC (6kg/ha) than in those soils without any previous exposure. This more rapid decomposition was evident after storage of soils for I year at ambient temperatures. Microorganisms were responsible for EPTC degradation in these soils. Some of the implications of this phenomenon on soil ecology are discussed.
Role of ghrelin gene-derived peptides in the control of energy balance and cardiometabolic homeostasis

The human GHRL gene encodes a 117-aminoacid peptide, preproghrelin that is proteolytically processed to yield two peptides: ghrelin and obestatin. Ghrelin is secreted to the bloodstream in two major forms, acylated ghrelin (with an n-octanoylation in the third serine residue) and desacyl ghrelin (the form lacking n-octanoylation). Ghrelin acylation, promoted by ghrelin O-acyltransferase (GOAT), is essential for binding to the growth hormone (GH) secretagogue receptor (GHS-R) 1a and for the main endocrine functions of acylated ghrelin, including the stimulation of GH release, induction of food intake and stimulation of adipogenesis. Although devoid of binding to GHS-R 1a, desacyl ghrelin also displays orexigenic and adipogenic actions, whereas the role of obestatin in the regulation of energy balance remains unclear. The discovery of the widespread distribution of ghrelin and its receptor in the cardiovascular system opened a new research field in the role of ghrelin in the control of blood pressure and myocardial function. Indeed, ghrelin inhibits the apoptosis of cardiomyocytes and endothelial cells, ameliorates left ventricular function and reduces fibrosis after myocardial injury in experimental models. In humans, ghrelin improves endothelial function by increasing nitric oxide (NO) bioavailability, normalizes the altered balance between endothelin-1 and NO in patients with metabolic syndrome and exerts performance-enhancing effects on myocardial function of patients with chronic heart failure. This review focuses on advances in cardiometabolic effects of ghrelin gene-derived products in rodents and humans, and the possible role of ghrelin as a therapeutic molecule for treating cardiometabolic diseases. Adipobiology 2011; 3: 21-30.
La Sporogenèse chez la Coccidie Aggregata eberthi. Etude en Microscopic Electronique

SYNOPSIS. The structure of the oocyst and formation of sporozoites of Aggregata eberthi were studied with the electron microscope. After penetration of the microgamete, a cyst wall containing fine projections is formed beneath the “anhist” layer which is pushed away. The cytoplasm is retracted beneath the cyst wall and is irregular in outline. Lipid inclusions are abundant, while paraglycogen is less so. Vacuoles present in the early stages of development may be instrumental in elaboration of the cyst wall. Granulations appear in the early oocyst cytoplasm and form large compact masses in the sporoblasts, assuming a crystalline appearance (crystalloid) in the sporozoites. The sporoblasts are separated by the coalescence of vesicles. Each sporoblast is surrounded by an epispore and a striated endospore which is perforated by the “dehiscence device. ” Three sporozoites of classical structure are formed in each sporoblast.