Miracle-dz/UniFAR-FTUs huggingface dataset:
Saliva—Friend and Foe in the COVID-19 Outbreak

The coronavirus disease 2019 (COVID-19) outbreak, caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global ongoing pandemic. Timely, accurate and non-invasive SARS-CoV-2 detection in both symptomatic and asymptomatic patients, as well as determination of their immune status, will facilitate effective large-scale pandemic control measures to prevent the spread of COVID-19. Saliva is a biofluid whose anatomical source and location is of particularly strategic relevance to COVID-19 transmission and monitoring. This review focuses on the role of saliva as both a foe (a common mode of viral transmission via salivary droplets and potentially aerosols) and a friend (as a non-invasive diagnostic tool for viral detection and immune status surveillance) in combating COVID-19.
Ovary and ovulation: DNA fragmentation of oocytes in aged mice

^To whom correspondence should be addressed To investigate whether female fertility decreases with age due to poor oocyte quality, we examined the presence of DNA fragmentation in ovulated oocytes from young, mature and aged mice. Oocytes from three age groups of female mice (7-8, 20-24 and 40-^48 weeks) were retrieved from the oviducts 15 h after human chorionic gonadotrophin (HCG) injection. Oocytes from each mouse were incubated in a CO2 incubator for 0-60 h in human tubal fluid (HTF). After incubation, each oocyte was stained with the terminal deoxynucleotidyl transferase-raediated dUDP nick-end labelling (TUNEL) method. The rate of DNA fragmentation (interpreted as apoptotic changes) was significantly higher for oocytes from aged mice, and the fertilization rate was significantly lower, compared with oocytes from young and mature mice. Our results suggest that DNA fragmentation of oocytes might be one of the reasons for poor oocyte quality and lower fertility hi the aged group.
Instigation of indigenous thermophilic bacterial consortia for enhanced oil recovery from high temperature oil reservoirs

The purpose of the study involves the development of an anaerobic, thermophilic microbial consortium TERIK from the high temperature reservoir of Gujarat for enhance oil recovery. To isolate indigenous microbial consortia, anaerobic baltch media were prepared and inoculated with the formation water; incubated at 65°C for 10 days. Further, the microbial metabolites were analyzed by gas chromatography, FTIR and surface tension. The efficiency of isolated consortia towards enhancing oil recovery was analyzed through core flood assay. The novelty of studied consortia was that, it produces biomass (600 mg/l), bio-surfactant (325 mg/l), and volatile fatty acids (250 mg/l) at 65°C in the span of 10 days, that are adequate to alter the surface tension (70 to 34 mNm -1) and sweep efficiency of zones facilitating the displacement of oil. TERIK was identified as Clostridium sp. The FTIR spectra of biosurfactant indicate the presence of N-H stretch, amides and polysaccharide. A core flooding assay was designed to explore the potential of TERIK towards enhancing oil recovery. The results showed an effective reduction in permeability at residual oil saturation from 2.14 ± 0.1 to 1.39 ± 0.05 mD and 19% incremental oil recovery.
Risk Response for Municipal Solid Waste Crisis Using Ontology-Based Reasoning

Many cities in the world are besieged by municipal solid waste (MSW). MSW not only pollutes the ecological environment but can even induce a series of public safety crises. Risk response for MSW needs novel changes. This paper innovatively adopts the ideas and methods of semantic web ontology to build an ontology-based reasoning system for MSW risk response. Through the integration of crisis information and case resources in the field of MSW, combined with the reasoning ability of Semantic Web Rule Language (SWRL), a system of rule reasoning for risk transformation is constructed. Knowledge extraction and integration of MSW risk response can effectively excavate semantic correlation of crisis information along with key transformation points in the process of crisis evolution through rule reasoning. The results show that rule reasoning of transformation can effectively improve intelligent decision-making regarding MSW risk response.
Anaerobic Power Assessment in Athletes: Are Cycling and Vertical Jump Tests Interchangeable?

Regularly assessing anaerobic power is important for athletes from sports with an explosive strength component. Understanding the differences and overlap between different assessment methods might help coaches or smaller-scale testing facilities maximize financial and temporal resources. Therefore, this study investigated the degree to which cycling sprint and vertical jump tests are interchangeable for determining peak mechanical leg power output in strength-trained athletes. Professional skiers (n = 19) performed unloaded squat jumps (SJ) and other jump forms on a force plate and a six-second cycling sprint (6sCS) test on an ergometer on six occasions over two years. Along with cross-sectional correlations between cycling and jumping power, correlations between longitudinal percent changes and agreement between magnitude-based inferences about individual changes were assessed. Among the tested jump forms, SJ reflected 6sCS best. However, despite extremely large cross-sectional correlation coefficients (0.92) between 6sCS and SJ, and moderate (Pearson’s r = 0.32 for 6sCS with SJ over one-year time spans) to large (r = 0.68 over shorter time spans) correlation coefficients on percent changes, magnitude-based inferences agreed in only around 50% of cases. Thus, for making qualitative assessments about the development of anaerobic power over time in athletes, cycling sprint and squat jump tests are not interchangeable. Rather, we recommend employing the test form that best reflects athletes’ strength and conditioning training.
Adult Stem Cell-Derived Extracellular Vesicles in Cancer Treatment: Opportunities and Challenges

Adult stem cells (SCs) participate in tissue repair and homeostasis regulation. The relative ease of SC handling and their therapeutic effect has made of these cell popular candidates for cellular therapy. However, several problems interfere with their clinical application in cancer treatment, like safety issues, unpredictable pro-tumour effects, and tissue entrapment. Therefore cell-free therapies that exhibit SC properties are being investigated. It is now well known that adult SCs exhibit their therapeutic effect via paracrine mechanisms. In addition to secretory proteins, SCs also release extracellular vesicles (EV) that deliver their contents to the target cells. Cancer treatment is one of the most promising applications of SC-EVs. Moreover, SC-EVs could be modified to improve targeted drug delivery. The aim of the review is to summarise current knowledge of adult SC-EV application in cancer treatment and to emphasise future opportunities and challenges in cancer treatment.
Reconstructing the Pleistocene geography of the Aphelocoma jays (Corvidae)

Understanding historical distributions of species and evolving lineages has been a topic of considerable interest, yet methods used to date have not provided detailed, quantitative distributional hypotheses. Here, we present a technique based on models of species’ ecological niches and Pleistocene climate reconstructions that provides such hypotheses, providing the example of reconstructions for the Aphelocoma jays. We demonstrate in general a greater degree than expected of stability in jay species’ distributional areas back through at least the most recent glaciation event, and that existing patterns of genetic differentiation may date to before the Late Pleistocene glaciations. More generally, the method offers the potential for reconstructing historical distributions of species or lineages, and providing a detailed geographic framework for addressing many biogeographic and systematic questions.
Accuracy of Distance Recordings in Eight Positioning-Enabled Sport Watches: Instrument Validation Study

Background Elite athletes and recreational runners rely on the accuracy of global navigation satellite system (GNSS)–enabled sport watches to monitor and regulate training activities. However, there is a lack of scientific evidence regarding the accuracy of such sport watches. Objective The aim was to investigate the accuracy of the recorded distances obtained by eight commercially available sport watches by Apple, Coros, Garmin, Polar, and Suunto when assessed in different areas and at different speeds. Furthermore, potential parameters that affect the measurement quality were evaluated. Methods Altogether, 3 × 12 measurements in urban, forest, and track and field areas were obtained while walking, running, and cycling under various outdoor conditions. Results The selected reference distances ranged from 404.0 m to 4296.9 m. For all the measurement areas combined, the recorded systematic errors (±limits of agreements) ranged between 3.7 (±195.6) m and –101.0 (±231.3) m, and the mean absolute percentage errors ranged from 3.2% to 6.1%. Only the GNSS receivers from Polar showed overall errors <5%. Generally, the recorded distances were significantly underestimated (all P values <.04) and less accurate in the urban and forest areas, whereas they were overestimated but with good accuracy in 75% (6/8) of the sport watches in the track and field area. Furthermore, the data assessed during running showed significantly higher error rates in most devices compared with the walking and cycling activities. Conclusions The recorded distances might be underestimated by up to 9%. However, the use of all investigated sport watches can be recommended, especially for distance recordings in open areas.
Location-Specific Social Norms and Personal Approval of Alcohol Use are Associated with Drinking Behaviors in College Students

Abstract Background: Prior research on risky alcohol use points to drinking norms as predictors of drinking behavior. Most research to date has only explored global (versus context-specific) drinking norms as they relate to general drinking behavior. To better understand risky drinking behavior in students, how norms may vary across drinking environments should be considered. Objectives: We sought to explore differences in drinking norms (descriptive and injunctive), personal approval, and alcohol use across specific drinking locations and how these location-specific predictors combine to best predict alcohol consumption in home/dorm locations, bars, and parties. Methods: College student drinkers (N = 115, 76% female) participated in an anonymous online cross-sectional survey in 2015–2016 assessing personal and perceived drinking experiences and attitudes across various locations. Results: Alcohol use, descriptive norms of alcohol use, and injunctive norms of alcohol use (but not personal approval) varied across location. In addition, location-specific descriptive norms were associated with alcohol use in each drinking location, whereas location specific personal approval was associated with alcohol use only at home/dorm and bar locations. Furthermore, descriptive norms and personal approval of drinking in a given location predicted alcohol use in that same location, while norms or approval for other locations did not. Conclusion/Importance: Results highlight the importance of specificity of perceived drinking norms and personal approval for predicting location-specific alcohol use. These findings have implications for interventions, which may benefit from discussions of students’ preferred drinking locations and providing location-specific normative feedback.
A Reference List of Phenolic Compounds (Including Stilbenes) in Grapevine (Vitis vinifera L.) Roots, Woods, Canes, Stems, and Leaves

Due to their biological activities, both in plants and in humans, there is a great interest in finding natural sources of phenolic compounds or ways to artificially manipulate their levels. During the last decade, a significant amount of these compounds has been reported in the vegetative organs of the vine plant. In the roots, woods, canes, stems, and leaves, at least 183 phenolic compounds have been identified, including 78 stilbenes (23 monomers, 30 dimers, 8 trimers, 16 tetramers, and 1 hexamer), 15 hydroxycinnamic acids, 9 hydroxybenzoic acids, 17 flavan-3-ols (of which 9 are proanthocyanidins), 14 anthocyanins, 8 flavanones, 35 flavonols, 2 flavones, and 5 coumarins. There is great variability in the distribution of these chemicals along the vine plant, with leaves and stems/canes having flavonols (83.43% of total phenolic levels) and flavan-3-ols (61.63%) as their main compounds, respectively. In light of the pattern described from the same organs, quercetin-3-O-glucuronide, quercetin-3-O-galactoside, quercetin-3-O-glucoside, and caftaric acid are the main flavonols and hydroxycinnamic acids in the leaves; the most commonly represented flavan-3-ols and flavonols in the stems and canes are catechin, epicatechin, procyanidin B1, and quercetin-3-O-galactoside. The main stilbenes (trans-ε-viniferin, trans-resveratrol, isohopeaphenol/hopeaphenol, vitisin B, and ampelopsins) accumulate primarily in the woods, followed by the roots, the canes, and the stems, whereas the leaves, which are more exposed to environmental stresses, have a low concentration of these compounds. Data provided in this review could be used as (i) a metabolomic tool for screening in targeted and untargeted analyses and (ii) a reference list in studies aimed at finding ways to induce naturally occurring polyphenols on an industrial scale for pant and human disease control.
Biomechanics of graded running: Part I ‐ Stride parameters, external forces, muscle activations

Biomechanical alterations with graded running have only been partially quantified, and the potential interactions with running speed remain unclear. We measured spatiotemporal parameters, ground reaction forces, and leg muscle activations (EMG) in nineteen adults (10F/9M) running on an instrumented treadmills at 2.50, 3.33, and 4.17 m·s−1 and 0, ±5°, and ±10°. Step frequency illustrated a significant speed × grade interaction (P < .001) and was highest (+3%) at the steepest grade (+10°) and fastest speed (4.17 m·s−1) when compared to level running (LR) at the same speed. Significant interaction was also observed for ground reaction forces (all P ≤ .047). Peak ground reaction forces in the normal direction increased with running speed during downhill running (DR) only (+9% at −10° and 4.17 m·s−1). Impulse in the normal direction decreased at fastest speed and steepest DR (−9%) and uphill running (UR) (−17%) grades. Average normal loading rate increased and decreased at fastest speed and steepest DR (+52%) and UR (−28%) grades, respectively. Negative parallel impulse increased and decreased at fastest speed and steepest DR (+166%) and UR (−90%), respectively. Positive parallel impulse decreased and increased at fastest speed and steepest DR (−75%) and UR (+111%), respectively. EMG showed comparable u‐shaped curves across the grades investigated, although only a change in vastus lateralis and tibilias anterior activity was detectable at the steepest grades and fastest speed. Overall, running grade and speed significantly influences spatiotemporal parameters, ground reaction forces, and muscle activations.
Activation of Complement System in Henoch-Schönlein Purpura Nephritis

Abstract Introduction: We studied the association between Henoch-Schönlein purpura nephritis (HSPN) and complement system activation. Methods: We retrospectively reviewed the pathologic findings and medical records of 35 children and 12 adults with HSPN and compared the differences according to C4d positivity in three groups consisting of total 47 patients, 35 pediatric and 12 adult patients, respectively. C4d staining of renal biopsy was additionally performed at the time of diagnosis or retrospectively using archival biopsy material. Results: The overall rate of C4d positivity was 53.2%: 20 (57.1%) of the 35 children and five (41.7%) of the 12 adults. Among the groups there was no significant difference in the severity of proteinuria, renal function, presence of crescents or mesangial proliferation stratified by C4d positivity, unlike IgA nephropathy. Conclusions: We suggest that the activation of complement system is not correlated with the clinical or pathological severity of HSPN.
Perceived Control Predicts Symptom Status in Patients With Heart Failure.

BACKGROUND Symptom status is an important indicator of disease progression in patients with heart failure (HF). Perceived control is a target of most self-care interventions and is associated with better outcomes in HF; however, little is known about the relationship between perceived control and symptom status in patients with HF. OBJECTIVE The aims of this study were to (1) determine the relationship of perceived control to HF symptom status and (2) examine the associations of perceived control to self-care and of self-care to symptom status. METHODS A total of 115 patients with HF were included. Data on symptom status (Memorial Symptom Assessment Scale-HF), perceived control (Control Attitudes Scale-Revised), and self-care (Self-Care of Heart Failure Index) were collected. Other covariates included were age, gender, New York Heart Association class, comorbidity burden, and depressive symptoms. Multiple regression analyses were performed to analyze the data. RESULTS Lower perceived control predicted worse symptom status after controlling for covariates (P = .009). Other covariates predictive of worse symptom status were younger age, New York Heart Association class III/IV, and higher levels of depressive symptoms. Higher levels of perceived control were associated with better self-care (P = .044). Better self-care was associated with better symptom status (P = .038). CONCLUSIONS Lower levels of perceived control were independently associated with worse symptom status in patients with HF. Intervention strategies targeting perceived control should be tested to determine whether they could improve symptom status.
All-Optical Formation and Manipulation of Microbubbles on a Porous Gold Nanofilm

Microbubble generation and manipulation in aqueous environments are techniques that have attracted considerable attention for their microfluidic and biological applications. Ultrasonic and hydrodynamic methods are commonly used to form and manipulate microbubbles, but these methods are limited by the relatively low precision of the microbubble sizes and locations. Here, we report an all-optical method for generation and manipulation of microbubbles with ~100 nm precision by using “hot spots” on a porous gold nanofilm under the illumination of near-infrared focused laser beam. The microbubble diameter ranged from 700 nm to 100 μm, with a standard deviation of 100 nm. The microbubbles were patterned into two-dimensional arrays, with an average location deviation of 90 nm. By moving the laser beam, the microbubbles could be manipulated to a desired region. This work provides a controllable way to form and manipulate microbubbles with ~100 nm precision, which is expected to have applications in optofluidic and plasmonic devices.
Lasers in Transurethral Enucleation of the Prostate—Do We Really Need Them

The acronym EEP, coding for transurethral Endoscopic Enucleation of the Prostate, was introduced in 2016 by the European Association of Urology (EAU) guidelines panel on management of non-neurogenic male lower urinary tract symptoms (LUTS) and benign prostatic obstruction (BPO). Since then, a laser-based treatment, Holmium Laser Enucleation of the Prostate (HoLEP), and the current-based treatment of bipolar enucleation of the prostate (BipoLEP) are equally appreciated as valuable options for the management of benign prostatic obstruction (BPO). This was mainly inspired by the results of two meta-analyses on randomized controlled trials, comparing open prostatectomy with either Holmium Laser Enucleation of the Prostate (HoLEP) or bipolar enucleation of the prostate (BipoLEP). Prior to that, HoLEP was embraced as the only valid option for transurethral enucleation, although evidence for equivalence existed as early as 2006, but was not recognized due to a plethora of acronyms for bipolar energy-based treatments and practiced HoLEP-centrism. On the other hand, the academic discourse focused on different (other) laser approaches that came up, led by Thulium:Yttrium-Aluminum-Garnet (Tm:YAG) Vapoenucleation (ThuVEP) in 2009 and, finally, transurethral anatomical enucleation with Tm:YAG support (thulium laser enucleation of the prostate, ThuLEP) in 2010. Initially, the discourse on lasers focused on the different properties of lasers rather than technique or surgical anatomy, respectively. In and after 2016, the discussion ultimately moved towards surgical technique and accepting anatomical preparation as the common of all EEP techniques (AEEP). Since then, the unspoken question has been raised, whether lasers are still necessary to perform EEP in light of existing evidence, given the total cost of ownership (TCO) for these generators. This article weighs the current evidence and comes to the conclusion that no evidence of superiority of one modality over another exists with regard to any endpoint. Therefore, in the sense of critical importance, AEEP can be safely and effectively performed without laser technologies and without compromise.
How to optimise urinary continence in anatomical endoscopic enucleation of the prostate?

The traditional transurethral resection of the prostate (TURP) is considered as gold‐standard surgical treatment to relieve symptoms resulting from bladder outlet obstruction by prostate enlargement. However, with the advances of novel laser technologies and more experienced surgeon conquering the steep learning curve, anatomical endoscopic enucleation of prostate (AEEP) has become a more popular alternative surgical technique. Although AEEP has compatible functional outcome, less blood loss, shorter catheterisation duration and hospital stay, the risk of post‐operative urinary incontinence (UI) is often an issue of concern. In this review, we focus on discussion about risk factors related to increased incidence of UI, some surgical tips to avoid damaging external urinary sphincter and treatment strategies to facilitate recovery of urinary continence after surgery.
Timing of Initiation of Renal Replacement Therapy in Sepsis-Associated Acute Kidney Injury

Sepsis-associated acute kidney injury (SA-AKI) is a major issue in medical, surgical and intensive care settings and is an independent risk factor for increased mortality, as well as hospital length of stay and cost. SA-AKI encompasses a proper pathophysiology where renal and systemic inflammation play an essential role, surpassing the classic concept of acute tubular necrosis. No specific treatment has been defined yet, and renal replacement therapy (RRT) remains the cornerstone supportive therapy for the most severe cases. The timing to start RRT, however, remains controversial, with early and late strategies providing conflicting results. This article provides a comprehensive review on the available evidence on the timing to start RRT in patients with SA-AKI.