Miracle-dz/UniFAR-FTUs huggingface dataset:
Epidemiology of Gastrointestinal Parasitism in Pigs in Subtropical Hill Zone of Meghalaya

An epidemiological study on gastrointestinal parasitic infection s in pigs in a subtropical hill zone of Meghalaya was conducted from April, 2011 to March, 2012. The faecal samples of 2370 numbers of pigs were examined for the purpose and out of these, 806 (34.00%) were found positive for different gastrointestinal parasites either single or mixed infection s. The mean eggs per gram of faeces (EPG) were 1244.10 with higher load of infections during rainy season. The prevalence of different gastrointestinal parasites as recorded after microscopical examination of faeces were Ascaris suum (61.29%), Strongyle spp. (45.90%), Trichuris spp. (16.25%), Strongyloides spp. (9.30%) and Eimeria spp. (37.96%). The prevalence of infection was found more in unorganized pig farms as compared to organized pig farms which were recorded as 36.18% and 28.65% with mean EPG 1345.98 and 1056.12, respectively. The infection was higher in rainy (36.96%) and cool (36.44%) seasons as compared to cold (24.75%) and warm/hot (23.12%) seasons. The coproculture of positive samples revealed the presence of larvae of Oesophagostomum dentatum(82.54 %) and Strongyloides ransomi(17.45 %).
Effects of microbial evolution dominate those of experimental host-mediated indirect selection

Microbes ubiquitously inhabit animals and plants, often affecting their host’s phenotype. As a result, even in a constant genetic background, the host’s phenotype may evolve through indirect selection on the microbiome. ‘Microbiome engineering’ offers a promising novel approach for attaining desired host traits but has been attempted only a few times. Building on the known role of the microbiome on development in fruit flies, we attempted to evolve earlier-eclosing flies by selecting on microbes in the growth media. We carried out parallel evolution experiments in no- and high-sugar diets by transferring media associated with fast-developing fly lines over the course of four selection cycles. In each cycle, we used sterile eggs from the same inbred population, and assayed mean fly eclosion times. Ultimately, flies eclosed seven to twelve hours earlier, depending on the diet, but microbiome engineering had no effect relative to a random-selection control treatment. 16S rRNA gene sequencing showed that the microbiome did evolve, particularly in the no sugar diet, with an increase in Shannon diversity over time. Thus, while microbiome evolution did affect host eclosion times, these effects were incidental. Instead, any experimentally enforced selection effects were swamped by uncontrolled microbial evolution, likely resulting in its adaptation to the media. These results imply that selection on host phenotypes must be strong enough to overcome other selection pressures simultaneously operating on the microbiome. The independent evolutionary trajectories of the host and the microbiome may limit the extent to which indirect selection on the microbiome can ultimately affect host phenotype. Random-selection lines accounting for independent microbial evolution are essential for experimental microbiome engineering studies.
The first record of Monogenea (Plathelminthes) parasitic on char (Salmonidae: Salvelinus) from Kronotsky Lake (Kamchatka Peninsula), Russia

Monogeneans are recorded as parasites of fishes in Kronotsky Lake for the first time. Salmonchus sp. , Gyrodactylus birmani and Gyrodactylus sp. were found in char. Salmonchus sp. is similar to Salmonchus alaskensis (Price, 1937) and Salmonchus grumosus (Pugachev, 1984), but cannot be unambiguously identified as one of them. Gyrodactylus sp. is similar to Gyrodactylus brachymystacis Ergens, 1978 sensu You et al., 2006 in many features but different in details of the marginal hook sickle morphology. How to cite this article: Sokolov S.G. , Gordeev I.I. 2014. The first record of Monogenea (Plathelminthes) parasitic on char (Salmonidae: Salvelinus) from Kronotsky Lake (Kam- chatka Peninsula), Russia // Invert. Zool. Vol.11. No.2. P.353-359.
Foliar application of abscisic acid mitigates cadmium stress and increases food safety of cadmium-sensitive lettuce (Lactuca sativa L.) genotype

Cadmium (Cd2 +) is among the toxic non-essential heavy metals that adversely affect plants metabolic processes and the safety of produce. However, plant hormones can improve plant’s tolerance to various stresses. This study investigated the effect of exogenous abscisic acid (ABA) on the biochemical and physiological processes and food safety of cadmium (Cd2 +)-sensitive lettuce genotype (Lüsu). Seedlings were subjected to five treatments: [(i) Control (untreated plants), (ii) 100 µM CdCl2, (iii) 100 µM CdCl2+10 µg L−1 ABA (iv) 10 µg L−1 ABA, and (v) 0.01 g L−1 ABA-inhibitor (fluridone)] for fourteen days in hydroponic system. The 100 µM CdCl2 increased the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), decreased photosynthesis and plant biomass. Moreover, it decreased the contents of essential nutrients (except copper) in the leaves but increased the contents of toxic Cd2 + in the leaves and roots of the plants. Foliar application of fluridone (0.01 g L−1) also caused oxidative stress by increasing the contents of H2O2 and MDA. It also decreased the contents of nutrient elements in the leaves of the plants. However, exogenous ABA (10 µg L−1) mitigated the Cd2 +-induced stress, increased antioxidant enzymes activities, photosynthesis and plant biomass under CdCl2 treatment. Remarkably, exogenous ABA increased the contents of essential nutrient elements but decreased the Cd2 + content in leaves under the CdCl2 treatment. Our results have demonstrated that foliar application of ABA mitigates Cd2 + stress and increases the nutritional quality and food safety of Cd2 +-sensitive lettuce genotype under CdCl2 treatment.
Organ Weights and Growth Profiles in Bottlenose Dolphins (Tursiops truncatus) from the Northwestern Gulf of Mexico

Systematic necropsies were performed on 63 bottlenose dolphins (Tursiops truncatus), and data on organ mass, standard body length (SBL), body mass (BM), gender, sexual maturity, and age were measured and/or estimated. Animals were extremely fresh and recovered from along the Texas and Louisiana coastline in the northwestern Gulf of Mexico. Organ reference tables were established for this species to facilitate comparisons with other bottlenose dolphins and to provide a baseline for other cetacean species. Organs examined included lungs, adrenal glands, kidneys, testes, ovaries, heart, liver, pancreas, brain, pituitary, thyroid, thymus, and spleen. Individuals were separated into three size classes: 225 cm, based on SBL to further facilitate comparisons. Growth rates of length and mass were described using Gompertz nonlinear models as a function of gender. No sexual dimorphism was identified in BM or organ weights, and SBL was only significantly larger for older mature males. SBL and BM were strongly correlated with age when all animals were included in analyses, although this is not an accurate predictor of age, especially in older individuals. Organ weights were significantly correlated with both SBL (except thymus and spleen) and BM (except left ovary, spleen, and thymus). Age was significantly correlated with all organ weights (except thymus, thyroid, and ovaries). There were no significant differences in the weight of any paired organs (adrenal glands, kidneys, lungs, ovaries, testes), and all were significantly correlated with BM. These data on organ weights of bottlenose dolphins, when interpreted with SBL, BM, and age, are significant tools for pathologists and veterinarians interpreting animal health status.
Editorial Perspective: COVID‐19 pandemic‐related psychopathology in children and adolescents with mental illness

The coronavirus disease (COVID‐19) pandemic is likely to have negative health consequences way beyond those caused by the virus per se – including significant psychological distress. Children and adolescents who already live with a mental illness may be particularly vulnerable to the distress associated with the pandemic – due to, for example, fear of the virus as well as the significant societal changes launched to minimize spread of the virus (social distancing and quarantine). In this editorial perspective, we (a) provide data on COVID‐19 pandemic‐related psychopathology in children and adolescents from a large psychiatric treatment setting in Denmark, (b) give advice on how the likely harmful effects of the COVID‐19 pandemic on the mental health of children and adolescents may be minimized, and (c) propose six lines of research into pandemic‐related psychopathology with emphasis on children and adolescents. Finally, we underline the necessity of politicians, health authorities, and funding bodies supporting these research initiatives here and now.
North-east Victoria SGS National Experiment site: water and nutrient losses from grazing systems on contrasting soil types and levels of inputs

Water and nutrient losses, pasture and animal production were measured for a prime lamb enterprise at Maindample (rainfall 750 mm/year) and a wool enterprise at Ruffy (rainfall 671 mm/year) in north-east Victoria from 1998 to 2000. Each site comprised 3 paddock-scale treatments: control, unsown pasture receiving about 5 kg phosphorus (P)/ha.year; medium input, sown pasture (about 10 kg P/ha.year); and high input, sown pasture (≥22 kg P/ha.year). Sown pastures were based on phalaris (Phalaris aquatica L.) at Maindample and cocksfoot (Dactylis glomerata L.) at Ruffy, and sheep were set stocked. Pastures at Maindample created a larger soil water deficit (commonly about 120 mm) compared with Ruffy (about 70 mm) in summer. Maximum soil water deficit at Maindample occurred under the high input pasture and was about 30 mm greater than the medium or control treatments. Phalaris content ranged between about 200 and 1300 kg DM/ha, between 10 and 70% of total composition (commonly about 20%). In contrast, at Ruffy the control, which had 20-40% native grasses (Austrodanthonia and Microlaena) achieved the greatest soil water deficit, about 25 mm greater than for sown pastures. Cocksfoot persisted poorly at the acid soil at Ruffy. Small differences in green leaf area over summer (about 200 kg DM/ha) between treatments could generate the soil water deficit differences over the summer. The most striking result was the markedly different pathways of water loss between surface losses and deep drainage at the 2 sites, which was of greater consequence than the effect of pasture type. At Maindample, on average, 166 mm of water was lost annually (22% of rainfall) with 110 mm of this as surface runoff. At Ruffy, annual water loss was 128 mm (19% of rainfall) with 110 mm of this total water loss estimated as loss through deep drainage. Phosphorus losses were low in all treatments (≤1 kg P/ha.year), and nitrate-N (NO 3 -N) losses (7-12 kg N/ha.year) were comparable with previous work. Concentrations of P in water were highest from the high treatments at both sites, averaging 0.91 and 0.83 mg P/L in surface runoff from Maindample high and Ruffy high treatments, respectively. Average soil water NO 3 -N concentrations ranged from 3 to 26 mg N/L. Both P and N concentrations were higher than acceptable for aquatic health. Environmental risks in terms of water and nutrient losses could be either higher or lower for sown than unsown pastures, depending upon soil type, botanical stability, persistence and the soil water extracting ability of the pasture. Results indicated that better environmental outcomes could be achieved if soil types were targeted for particular land uses. High management skills are needed if grazing enterprises are to be both profitable and have lower off-site impacts.
Follow-Up in Bone Sarcoma Care: A Cross-Sectional European Study

Background Follow-up of high-grade bone sarcoma patients with repeated radiological imaging aims at early detection of recurrent disease or distant metastasis. Repeated radiological imaging does expose (mostly young) patients to ionising radiation. At this point, it is not known whether frequent follow-up increases overall survival. Additionally, frequent follow-up subjects patients and families to psychological stress. This study aims to assess follow-up procedures in terms of frequency and type of imaging modalities in bone tumour centres across Europe for comparison and improvement of knowledge as a first step towards a more uniform approach towards bone sarcoma follow-up. Methods Data were obtained through analysis of several follow-up protocols and a digital questionnaire returned by EMSOS members of bone tumour centres all across Europe. Results All participating bone tumour centres attained a minimum follow-up period of ten years. National guidelines revealed variations in follow-up intervals and use of repeated imaging with ionising radiation. A local and a chest X-ray were obtained at 47.6% of the responding clinics at every follow-up patient visit. Conclusions Variations were seen among European bone sarcoma centres with regards to follow-up intervals and use of repeated imaging. The majority of these expert centres follow existing international guidelines and find them sufficient as basis for a follow-up surveillance programme despite lack of evidence. Future research should aim towards evidence-based follow-up with focus on the effects of follow-up strategies on health outcomes, cost-effectiveness, and individualised follow-up algorithms.
Optimum Boiling Duration and Its Effect on Nutritional Quality and Acceptability of Mechanically Dehulled Unfermented Locust Bean Seeds

Most locust bean processing is still carried out locally in Africa. Dehulling is one of the major challenges encountered in traditional processing of locust bean seeds. Boiling time prior to dehulling is vital for nutritional status of locust bean. Hence, this study explored the influence of boiling duration before dehulling on the nutritional quality of mechanically dehulled locust bean seeds. The samples were subjected to four different boiling durations (1∼4 h) and the resulting effects on the overall acceptability, proximate composition, mineral content, and pH were evaluated. Locust beans boiled for 2 h and dehulled at the speed of 398 rpm gave the highest crude protein content, crude fiber, crude fat, and ash content. The pH ranged from 5.48 to 5.77, while boiling improved the mineral content ranging from 0.25∼0.48 mg/100 g (potassium), 16.80∼28.00 mg/100 g (calcium), 3.85∼6.73 mg/100 g (sodium), and 40.00∼52.40 mg/100 g (magnesium). The tedious labour during dehulling of locust bean seeds can be reduced at 398 rpm dehulling speed without adversely affecting the slightly acidic status and nutritional quality; thus enhancing quality and overall acceptability. Adoption of boiling raw locust bean seeds for 2 h under pressure prior to dehulling is a valuable procedure to eliminate long hours of boiling and tedious labour during local and industrial production.
Factors Influencing Success Rate of Intracytoplasmic Sperm Injection with Azoospermic Male Patients

Introduction Azoospermia affects about 1% of men, of whom up to 15% inquire about infertility treatment. Information about predictive factors for these couples is very limited. Patients, Materials and Methods We performed a retrospective analysis of the clinical records of 118 cycles of intracytoplasmic sperm injection treatment after testicular sperm extraction for male azoospermia carried out between January 2008 and October 2015. Of those, 66 were first, 35 second, and 17 third cycles. Statistical significance was set at p < 0.05. Predictive factors for successful pregnancy were evaluated and included male/female age, male/female body mass index, male/female nicotine use, and histological results of testes biopsies. Results Embryo quality and the number of embryos transferred were positively associated with pregnancy success (p = 0.003). Males whose partners conceived had a significantly lower body mass index than those whose partners did not conceive (p = 0.023). Neither female weight nor age nor smoking status of the male or female were significant factors. In cases with tubular atrophy ≥ SIGG grade 4 the chance of pregnancy was poor, irrespective of the existence of mature sperm and the number of cycles performed. Conclusion Overweight male patients should be advised about weight reduction prior to treatment, and counseling about success rates should include histological and sperm-positive biopsy results.
A Novel Connectome-based Electrophysiological Study of Subjective Cognitive Decline Related to Alzheimer’s Disease by Using Resting-state High-density EEG EGI GES 300

Aim: To investigate for the first time the brain network in the Alzheimer’s disease (AD) spectrum by implementing a high-density electroencephalography (HD-EEG - EGI GES 300) study with 256 channels in order to seek if the brain connectome can be effectively used to distinguish cognitive impairment in preclinical stages. Methods: Twenty participants with AD, 30 with mild cognitive impairment (MCI), 20 with subjective cognitive decline (SCD) and 22 healthy controls (HC) were examined with a detailed neuropsychological battery and 10 min resting state HD-EEG. We extracted correlation matrices by using Pearson correlation coefficients for each subject and constructed weighted undirected networks for calculating clustering coefficient (CC), strength (S) and betweenness centrality (BC) at global (256 electrodes) and local levels (29 parietal electrodes). Results: One-way ANOVA presented a statistically significant difference among the four groups at local level in CC [F (3, 88) = 4.76, p = 0.004] and S [F (3, 88) = 4.69, p = 0.004]. However, no statistically significant difference was found at a global level. According to the independent sample t-test, local CC was higher for HC [M (SD) = 0.79 (0.07)] compared with SCD [M (SD) = 0.72 (0.09)]; t (40) = 2.39, p = 0.02, MCI [M (SD) = 0.71 (0.09)]; t (50) = 0.41, p = 0.004 and AD [M (SD) = 0.68 (0.11)]; t (40) = 3.62, p = 0.001 as well, while BC showed an increase at a local level but a decrease at a global level as the disease progresses. These findings provide evidence that disruptions in brain networks in parietal organization may potentially represent a key factor in the ability to distinguish people at early stages of the AD continuum. Conclusions: The above findings reveal a dynamically disrupted network organization of preclinical stages, showing that SCD exhibits network disorganization with intermediate values between MCI and HC. Additionally, these pieces of evidence provide information on the usefulness of the 256 HD-EEG in network construction.
Outdoor recreation planning: a comprehensive approach to understanding use interaction.

Growing demands for outdoor recreation have led to increased levels of crowding, displacement, competition and antagonism among alternative recreational uses. Recreational activities interact with differing degrees of compatibility resulting in various outcomes. In this review, we critique the relevant literature on recreation conflict, use compatibility and additivity with a focus on the interaction that takes place among alternative recreational uses. Results suggest that the literature is replete with theoretical and empirical studies focused on recreational conflict, while more comprehensive interaction types remain less studied and, in general, lacking overarching conceptual and analytical approaches. Further, there exists a need to link comprehensive approaches that address recreational use interaction with empirical analyses and management-specific strategies that allow for proactive planned outcomes that are positive-sum. We forward an approach to recreation planning that focuses on managing for relative compatibilities that involves maximizing those uses thought to be complementary and supplementary, while segregating and/or regulating those uses that are competitive and antagonistic.
The Association between Serum Uric Acid and Bone Mineral Density in Older Adults

Objectives Uric acid has been found to be potentially protective in bone metabolism. We investigated the relationship between serum uric acid (sUA) and lumbar bone mineral density (BMD) among 4156 participants aged 60 years and over from the National Health and Nutrition Examination Survey (NHANES). Methods To estimate the association between sUA and lumbar BMD, multivariate logistic regression analyses were conducted. Fitted smoothing curves and generalized additive models were also performed. Results We found sUA positively correlated with lumbar BMD after adjusting for other confounders. On subgroup analyses, stratified by sex and race/ethnicity, the positive correlation of sUA with lumbar BMD remained in both men and women, as well as in whites and Mexican Americans, but not in blacks. In blacks, the association of sUA with lumbar BMD was an inverted U-shaped curve (inflection point: 7.5 mg/dL). Conclusions Our study revealed a positive relationship between sUA and lumbar BMD among most old adults. This association followed an inverted U-shaped curve among blacks.
Self-Medication among Medical and Nonmedical Students at the University of Gondar, Northwest Ethiopia: A Cross-Sectional Study

Despite having some benefits, self-medication increases risks such as unnecessary use of medication, extended duration of consumption, incorrect diagnosis, drug-drug interactions, and polypharmacy. Thus, the purpose of this study is to compare self-medication practice between medical and nonmedical students of the University of Gondar, Northwest Ethiopia. An institutional-based cross-sectional comparative study was conducted on medical and nonmedical students of the University of Gondar from March 25 to May 15, 2018. A comparative sample of 213 medical and 212 nonmedical students were enrolled in the study. Data were collected by physically visiting the students in their campuses, using a semistructured questionnaire. Of the participants with a history of medication use in the past 12 months, 64.5% practiced self-medication. The prevalence of self-medication was 59.7% among medical students and 69.0% among nonmedical students. “Knowing the treatment of the disease” was the most frequent reason behind self-medication. Analgesics/antipyretics were the most common categories of medications used, whereas headache was the predominant ailment for which the medications were used. Self-medication practice was found to be higher in the fifth year students and nonmedical students (p < 0.05). In conclusion, self-medication is common among students of the University of Gondar. Nonmedical students were more likely to have practiced self-medication as compared to medical students.
Genetic Variability Among Isolates of Plesiomonas shigelloides from Fish, Human Clinical Sources and Fresh Water, Determined by RAPD Typing

Plesiomonas shigelloides has been a suspected pathogen for the past 40 years. Random amplified polymorphic DNA (RAPD) analysis with two random primers was used to examine the genetic diversity among 26 strains of P. shigelloides: 10 from fresh water, 6 from fish, and 10 from human clinical sources. There was notable genetic variability among most of the isolates, and none of the isolates had the same composite RAPD profile. Our results indicate that most of the isolates from the same source grouped together and that the isolates from fish had a closer linkage to the human clinical isolates than did the freshwater isolates, suggesting that fish may be the more serious source for potential risk of infection. We also found that certain human clinical isolates had almost the same RAPD profiles as certain of the isolates from freshwater and fish, indicating that P. shigelloides from both sea food and freshwater should be considered potential pathogens.