Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of MiR-9 promotes proliferation, migration and differentiation of human neural stem cells

Purpose: To investigate the effect of miR-9 on the proliferation, differentiation and migration of human neural stem cells (NSCs). Methods: The expression of miR-9 was investigated by quantitative real-time polymerase chain reaction (RT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK8) assay, while cell migration was studied by Transwell assay. The effect of miR-9 on differentiation of NSCs was investigated by western blot analysis of key differentiation marker proteins. Protein expression was determined by western blotting. Results: Transfection and over-expression of miR-9 in NSCs significantly enhanced the proliferation of NSCs (p < 0.05) in a time-dependent manner, as was evident from CCK8 assay data. MiR-9 overexpression caused down-regulation of Nestin and SOX-2, and up-regulation of Tuj-1 and MAP-2. The migration of NSCs was 37 % in the cells transfected with empty vector, compared to 68 % in the cells transfected with miR-9. This effect of miR-9 on cell migration was accompanied by up-regulation of matrix metallopeptidase 9 (MMP-9) and matrix metallopeptidase 2 (MMP-2). Conclusion: These results show that miR-9 promotes the proliferation, differentiation and migration of NSCs, and thus may be an important drug target for the generation of NSCs. Keywords: Neural stem cells, MicroRNA, Mir-9, Migration, Differentiation, Proliferation
A Reliable Method for Determining the Oxidation State of Manganese at the Microscale in Mn Oxides via Raman Spectroscopy

Manganese oxides are important geomaterials, widespread in terrestrial and Martian environments. Characterisation of the oxidation state of Mn is a central issue in science; this task has been addressed up to the present by X‐ray spectroscopy or diffraction techniques. The former, however, requires access to synchrotron facilities, while the latter does not provide crystal‐chemical information at the local scale. In this work, we compare a large set of Raman data from well‐characterised samples, already published by the same authors of this paper or as found in the literature. We show a clear correlation between the oxidation state of Mn and the wavenumber of peculiar bands; octahedrally co‐ordinated Mn2+ is recognised by a band around 530 cm−1, Mn3+ by a band around 580 cm−1 and Mn4+ by a band around 630 cm−1, while tetrahedrally co‐ordinated Mn2+ is recognisable by a band around 650 cm−1. Strongly distorted Mn3+ octahedra are indicated by the appearance of Jahn–Teller modes. Our method allows a reliable, easily accessible tool to characterise the oxidation states of Mn in oxides, also suitable for microscale mapping. It provides a robust analytical basis for the use of these minerals as redox indicators in geology/geochemistry, in exoplanetary research or for monitoring technological processes.
A Social-Belonging Intervention Benefits Higher Weight Students’ Weight Stability and Academic Achievement

Psychological interventions can narrow college achievement gaps between students from nonstigmatized and stigmatized groups. However, no intervention we know of has investigated effects for one highly stigmatized group: people of higher bodyweights. We analyzed data from a prematriculation social-belonging intervention trial at 22 colleges, which conveyed that adversity in the college transition is normative, temporary, and nondiagnostic of lack of belonging. Nine months postintervention, higher weight participants in a standard belonging treatment had higher first-year grade point averages (GPAs) than controls and maintained more stable weights, an indicator of physical well-being. Effects of a belonging treatment customized to specific colleges were directionally similar but nonsignificant. Exploratory analyses revealed that effects did not differ by race and that weight effects were driven by women. Together, results show that higher weight students contend with belonging concerns that contribute to a weight gap in GPA, but belonging interventions can raise GPA and promote healthy weight stability.
Estimating Phenotypic and Genotypic Path Coefficient, an Application on Wheat (Triticum aestivum L.) Genotypes

Wheat (Triticum aestivum L.) is one of the most important and strategically over the world. In Egypt, wheat is the main cereal crop used as stable food for urban and rural societies and the major source of straw for animal feeding. However, total wheat consumption has drastically increased due to over. Population growth by about 2.5% per year. Egypt imports about 45 % of its wheat requirement. This reflects size of the problem and the efforts needed to increase wheat population. Thus, increasing production per unit area appears to be one of the important factors for narrowing the gab between wheat production and consumption.
Relative contribution to the shark fin trade of Indo‐Pacific and Eastern Pacific pelagic thresher sharks

Fins from highly mobile shark species entering large shark fin trade hubs can originate from various geographical locations and stocks. Tracing fins from internationally regulated species to their starting point in the supply chain can have important implications for the monitoring and enforcement of international laws intended to protect threatened shark species. Here we use pelagic thresher shark Alopias pelagicus fin samples (N = 86) collected during a 4‐year period (2014–2017), and phylogenetic analyses, using the cytochrome oxidase I (COI), to determine the relative contribution of this species to the two largest shark fin trade hubs in the world, Hong Kong and Guangzhou, mainland China. Our results suggest that the Eastern Pacific is a major starting point of the international supply chain of pelagic thresher shark fins, contributing 84.9% of the analyzed market‐derived fin samples. We suggest that capacity building investments are needed in Eastern Pacific countries to increase the efficiency of border control inspections and improve the enforcement of international trade regulations for this threatened shark species. Our study highlights the utility of combining population genetics data and large‐scale market surveys to trace wildlife products back to the starting point in the supply chain and support the assessment of emerging management and conservation measures for exploited sharks.
Detachment of adhered enteropathogenic Escherichia coli cells from polythene fragments immersed in aquatic microcosm using Eucalyptus microcorys extract (Myrtaceae)

This study aimed to assess the effect of the concentration of an aqueous extract of Eucalyptus microcorys on the detachment of enteropathogenic E. coli cells previously adhered on polythene surface. The results showed that the extract causes a detachment of cells adhered and the importance of detachment depends on incubation duration of substrate in the plant extract solution. The higher value of detached cell abundance, after 3 hours duration of cell adhesion process (DCAP), was 8.72 (Ln (CFU/cm2)) and the percentage of detached cells reached value of 11.2% when the bacteria cells were harvested from the lag growth phase. For cells coming from exponential growth phase, after 9h of DCAP, the abundance of cells detached reached value of 8.95 (Ln (CFU/cm2)) and the higher percentage of detached cells was 68.8%. The values of 48.5% and 55.6% were noted when cells harvested from stationary and decline growth phases respectively. The cell detachment process observed could be due to the presence of some secondary metabolites such as flavonoids, triterpenoids, alkaloids, tannins which can disrupt physicochemical characteristics of bacteria surface. They seem to have a significant impact on exopolysaccharids matrix secreted by bacteria during the cell adhesion process. An overall significant difference (P<0.01) was observed between the mean abundances of detached cells for each extract concentrations at different growth stages.
Designing inquiry-based learning environments for quantum physics education in secondary schools

This paper describes the design process for a digital instructional sequence on introductory quantum physics for upper secondary education. Based on a collaboration between teachers and physics education researchers, this sequence incorporates relevant theoretical foundations from the field of science teaching to promote meaningful and conceptual learning of quantum physics. The sequence is composed of units, which are being developed using the Go-Lab ecosystem (www.golabz.eu), a free online platform for teachers to create digital inquiry-based lessons. So far, the sequence covers the photoelectric effect, wave-particle duality, and tunnelling phenomena. This paper focuses on the sequence’s first unit, addressing the photoelectric effect. The unit is used in this paper to exemplify the incorporation of the sequence’s theoretical foundations: digital inquiry-based learning with simulations, collaborative learning, and conceptual change. The unit was pilot-tested with 114 students in four Dutch high schools. Answers to multiple-choice and open-ended questions were collected through the Go-Lab platform. Analyses showed the unit’s potential to promote correct reasoning about the role of the intensity and frequency variables and revealed students’ difficulties in grasping light’s particle nature at the end of the lesson. These preliminary results are used to draw conclusions about how to improve this unit and inform the design of new instructional sequences.
Perceived Organizational Support and Employee Creativity: The Mediation Role of Calling

ABSTRACT The current study identified one of the factors that contribute to employee creativity. In addition, it examined the relationship among perceived organizational support, calling, and employee creativity. A total of 410 employees from 68 organizations were included in the sample. The short version of the Survey of Perceived Organizational Support, Calling and Vocation Questionnaire, and Person–Environment Fit Scale for Creativity was administered. Results confirmed that perceived organizational support, calling, and employee creativity are positively and significantly correlated. Perceived organizational support and calling were relevant contributors to employee creativity. The mediating role of calling (i.e., the purposeful work dimension of calling) between perceived organizational support and employee creativity was discussed. These findings may explain how perceived organizational support can affect employee creativity. In addition, they may help unlock employee creativity via perceived organizational support and calling.
Asymmetrical localization of Nup107-160 subcomplex components within the nuclear pore complex in fission yeast

The nuclear pore complex (NPC) forms a gateway for nucleocytoplasmic transport. The outer ring protein complex of the NPC (the Nup107-160 subcomplex in humans) is a key component for building the NPC. Nup107-160 subcomplexes are believed to be symmetrically localized on the nuclear and cytoplasmic sides of the NPC. However, in S. pombe immunoelectron and fluorescence microscopic analyses revealed that the homologous components of the human Nup107-160 subcomplex had an asymmetrical localization: constituent proteins spNup132 and spNup107 were present only on the nuclear side (designated the spNup132 subcomplex), while spNup131, spNup120, spNup85, spNup96, spNup37, spEly5 and spSeh1 were localized only on the cytoplasmic side (designated the spNup120 subcomplex), suggesting the complex was split into two pieces at the interface between spNup96 and spNup107. This contrasts with the symmetrical localization reported in other organisms. Fusion of spNup96 (cytoplasmic localization) with spNup107 (nuclear localization) caused cytoplasmic relocalization of spNup107. In this strain, half of the spNup132 proteins, which interact with spNup107, changed their localization to the cytoplasmic side of the NPC, leading to defects in mitotic and meiotic progression similar to an spNup132 deletion strain. These observations suggest the asymmetrical localization of the outer ring spNup132 and spNup120 subcomplexes of the NPC is necessary for normal cell cycle progression in fission yeast.
The Role of Health Workers and Community Leaders to Prevent Dengue Hemorrhagic Fever in Magetan, East Java

ABSTRACT Background: Dengue Hemorrhagic Fever (DHF) is still one of the major public health problems in Indonesia. With the increasing mobility and population density, the number of people and vast distribution area is increasing. Village of Tawanganom is one endemic region, for three consecutive years, there is an incidence of dengue. The incidence of dengue can be influenced by several aspects, including vectors, climate change, environment, mobility of people, and people's behavior. The participation of health workers and community leaders become important related behavior. These include the role of motivation, coordination, policy implementation, as well as healthy behaviors. Objective: This study aimed to determine the role of health workers and community leaders in response to the incidence of dengue in the Village Tawanganom. Methods: Using qualitative research with a phenomenological approach, as well as in-depth interviews carried out with supporting data, the number of informants were 13 informants. Consisting of health professionals that the holder of dengue program, Health Promotor, and village midwives, community leaders the Head of the village, RW, as well as health cadres. Results: The motivation of public figures came from the concern over them because of the many cases of DHF and personal experiences of informants and families. Health workers motivate people to do counseling, which was supported because of responsibility as health professionals. Coordination has been made with the relevant sectors. Reporting cases of executed massively and focused. Implementation of policies in the prevention of dengue fever has been carried out based dengue prevention program Magetan District Health Office. Healthy behavior is shown with dengue prevention measures such as 3M Plus, giving abate powder in the bathroom, as well as maintaining the cleanliness of the home environment. Conclusion: The motivation to do a public figure driven intrinsic motivation of the individuals themselves, while medical personnel with extrinsic motivation based on the responsibility as health workers to encourage people to do the prevention of dengue. Coordination has been carried out massively and regularly within the scope of cross-fertilization. Implementation of policies based on the program of the Health Service Magetan. Healthy behavior implemented preventive and promotive measures.
Encouraged or Discouraged? The Effect of Adverse Macroeconomic Conditions on School Leaving and Reentry

Existing research generally confirms a countercyclical education enrollment, whereby youths seek shelter in the educational system to avoid hardships in the labor market: the “discouraged worker” thesis. Alternatively, the “encouraged worker” thesis predicts that economic downturns steer individuals away from education because of higher opportunity costs. This study provides a formal test of these opposing theories using data from the United States compared with similar sources from the United Kingdom, Germany, and Sweden. I investigate whether macroeconomic stimuli—including recessions and youth unemployment fluctuations—matter for enrollment decisions. Analyses rely on 10 years of detailed individual-level panel data, consisting of birth cohorts across several decades. Across data sources, results show enrollment persistence in secondary education is stronger in response to economic downturns. These patterns differ sharply for tertiary-enrolled students and those who recently left higher education. Surprisingly, U.S. youths display an increased hazard of school leaving and a decreased hazard of educational reenrollment in response to adverse conditions. In contrast, European youths tend to make enrollment decisions supportive of discouraged-worker mechanisms or insensitivity to adverse conditions. The U.S.-specific encouraged-worker mechanism might be explained by the relative importance of market forces in one’s early career and the high costs of university attendance, which induces risk aversion with regard to educational investment. The discussion addresses the consequences for educational inequality.
Women’s Participation in Education and Politics: Evidence from the Selected OIC Countries

In this twenty-first century, it is crystal clear that education is the most powerful instrument for a nation’s progress and prosperity. Islam has also emphasized on educating both men and women since the beginning. So, Muslim countries should take initiatives to enhance participation of women in education. In reality, it is seen that women’s positions in the field of education and politics are very nominal compared to their male counterparts within Muslim countries. Situation is even worse in under-developed and non-democratic countries where the position of women and their participation in both the education and political sectors alongside men are visibly low. In this context, the purpose of this study is to explore the level of Muslim women’s participation in education and politics in some selected Muslim countries. Two South-Asian countries, Bangladesh and Pakistan, two south-east Asian countries, Malaysia and Indonesia, and the Middle East and North Africa (MENA) countries have been considered for this study. Thus, empowered women can take part in politics to resolve social issues. One of the findings of this study is that in democratic countries such as; Malaysia, Indonesia, and Bangladesh, women’s participation in both the educational and political sectors have been significantly improving over the years. Though economic conditions and social stigma, directly and indirectly, are still the barriers to enhance women’s education level as well as their participation in politics. Finally, the study highlights the obstacles that hinder women’s participation in both sectors and discusses the remedies to overcome the situation from an Islamic point of view.
A highly diverse coenagrionoid damselfly group (Odonata: Zygoptera: Burmacoenagrionidae fam. nov.) from mid-Cretaceous Burmese amber

The damselfly superfamily Coenagrionoidea is the largest zygopteran group, comprising three-fifths of all extant damselfly species. The Mesozoic fossil record of this superfamily is sparse, whilst it is relatively common in Burmese amber. A new coenagrionoid family, Burmacoenagrionidae Zheng et al., fam. nov., is established here based on four new species in three new genera: Burmacoenagrion pretiosus Zheng et al. gen. et sp. nov., Burmachistigma cheni Zheng et al. gen. et sp. nov., Electrocoenagrion elongatum Zheng et al. gen. et sp. nov. and Electrocoenagrion forficatum Zheng et al. gen. et sp. nov. The previously described damselfly genus, Burmagrion Möstel et al., 2017, is transferred to this family. Burmacoenagrionidae Zheng et al. fam. nov. has a long pterostigma covering 3–5 cells, pigmented wings and a sigmoidally curved RA and RP1 distal of the pterostigma, differing from other coenagrionoid damselflies. Until now, this is the most diverse damselfly family reported from Burmese amber, showing that the Coenagrionoidea were already highly diversified 100 million years ago. http://zoobank.org/urn:lsid:zoobank.org:pub:DAB9700E-2F21-4BC7-97A0-41569DA4898A
The Rana Plaza disaster seven years on: Transnational experiments and perhaps a new treaty?

This article examines several noteworthy initiatives that were implemented following the deadly 2013 Rana Plaza disaster in Bangladesh. They broke new ground in transnational labour law. The ILO-backed initiatives were largely successful but remain insufficient to achieve lasting change in the ready-made garment industry, where global brands’ supply chain buying practices constrain investment in occupational safety and health. A proposed United Nations treaty on business and human rights now seeks to enhance corporate accountability. Although promising, as part of a smart mix of multi-level public and private solutions, the treaty needs fine-tuning in the light of lessons learned from post-Rana Plaza experiments.
Cyber situation perception for Internet of Things systems based on zero‐day attack activities recognition within advanced persistent threat

With the development of the Internet of Things (IoT) technology, various attacks and threats have emerged. The advanced persistent threat (APT) refers to a class of advanced multiple‐steps attacks among diverse attack activities, which brings severe threats to the IoT systems ascribe to its pertinence, concealment, and permeability. However, the existing technologies and methods fail to timely recognize the APT attack activities (especially the zero‐day exploits) in a comprehensive scope. To address this problem, we propose a novel method of cyber situation perception for IoT systems, which based on zero‐day attack activity recognition within APT (CSPAPTM). Moreover, we also design an edge computing framework for applying CSPAPTM to the typical IoT systems. Specifically, we first provide a cyber situation perception ontology construction module for describing the APT attack activities. Then, a malicious C&C DNS mining method (MCCDRM) is proposed to control the APT malicious activity correlation analysis trigger, which can effectively decrease the computing overhead. Finally, we propose a zero‐day attack activity recognition method within APT (ZDAARA), which acts on system call instances to recognize the malicious activities, which cannot be detected by IDS. A relatively mature access control mechanism PO‐SAAC is also applied to our method. Through the coalescent of these methods, CSPAPTM can accomplish the cyber situation perception effectively by the zero‐day attack activities recognition in the IoT systems. The exhaustive experimental results demonstrate that the two kernel modules, that is, MCCDRM and ZDAARA in our CSPAPTM, can achieve both higher F1 score and acceptable false positive rate.
Cloning and analysis of reverse transcriptases from Ty1-copia retrotransposons in Camellia sinensis

ABSTRACT As mobile genetic elements, the diversity and activity of the Tyl-copia retrotransposons are key contributors to genome organisation and evolution, which have been investigated in many plants but little in the tea plant, Camellia sinensis. We selected a total of 12 varieties of tea plant distributed across a large geographical area and sequenced the reverse transcriptase (RT) sequences of Ty1-copia retrotransposons using degenerate primers. The sequences that were widespread among tea varieties, were approximately 260 bp in length and exhibited high heterogeneity among tea plants. The RT sequences from tea plants were similar to other known Ty1-copia retrotransposon RT sequences. Phylogenetic analysis showed that tea RT sequences were closely related to those from woody plants, such as pear, poplar and apple. In contrast, they were more distantly related to RT sequences from herbaceous plants, such as tomato and rice. Activity assay revealed that Ty1-copia retrotransposons are transcriptionally active during the normal development of tea plants. These results will support further research on Ty1-copia retrotransposons in C. sinensis.