Miracle-dz/UniFAR-FTUs huggingface dataset:
Polymorphisms within the SARS-CoV-2 Human Receptor Genes Associate with Variable Disease Outcomes across Ethnicities

The contribution of human genes to the variability of disease outcomes has been shown to be important across infectious diseases. Studies have shown mutations within specific human genes are associated with variable COVID-19 outcomes. We focused on the SARS-CoV-2 receptors/co-receptors to identify the role of specific polymorphisms within ACE2, TMPRSS2, NRP1 and CD147. Polymorphisms within ACE2 (rs2285666), TMPRSS2 (rs12329760), CD147 (rs8259) and NRP1 (rs10080) have been shown to associate with COVID-19 severity. Using cryopreserved samples from COVID-19-positive African, European and South Asian individuals within South Africa, we determined genotype frequencies. The genetic variant rs2285666 was associated with COVID-19 severity with an ethnic bias. African individuals with a CC genotype demonstrate more severe COVID-19 outcomes (OR = 7.5; 95% CI 1.164–80.89; p = 0.024) compared with those with a TT genotype. The expressions of ACE2 and SARS-CoV-2 viral load were measured using droplet digital PCR. Our results demonstrate rs2285666 and rs10080 were significantly associated with increased SARS-CoV-2 viral load and worse outcomes in certain ethnicities. This study demonstrates two important findings. Firstly, SARS-CoV-2 viral load is significantly lower in Africans compared with individuals of European and South Asian descent (p = 0.0002 and p < 0.0001). Secondly, SARS-CoV-2 viral load associates with specific SARS-CoV-2 receptor variants. A limited number of studies have examined the receptor/co-receptor genes within Africa. This study investigated genetic variants within the SARS-CoV-2 receptor/co-receptor genes and their association with COVID-19 severity and SARS-CoV-2 viral load across different ethnicities. We provide a genetic basis for differences in COVID-19 severity across ethnic groups in South Africa, further highlighting the importance of further investigation to determine potential therapeutic targets and to guide vaccination strategies that may prioritize specific genotypes.
Mitigating Human Impacts on Wild Animal Welfare

Simple Summary This paper considers examples of how humans negatively affect the welfare of wild animals, how such impacts might be reduced and what additional research is needed, including examples from biodiversity conservation, wildlife management, wildlife tourism and wildlife trade. Further, it discusses the relationship between wild animal welfare and biodiversity conservation, including consideration of various ethical viewpoints. Abstract Human activities negatively impact the welfare of wild vertebrates in many different contexts globally, and countless individual animals are affected. Growing concern for wild animal welfare, especially in relation to conservation, is evident. While research on wild animal welfare lags behind that focused on captive animals, minimising human-induced harm to wild animals is a key principle. This study examines examples of negative anthropogenic impacts on wild animal welfare, how these may be mitigated and what further research is required, including examples from wildlife management, biodiversity conservation, wildlife tourism and wildlife trade. Further, it discusses the relationship between animal welfare and biodiversity conservation, and synergies that may be achieved between these. Ultimately, it is discussed how the welfare of wild animals may be balanced with other priorities to ensure that welfare is afforded due consideration in interactions between people and wildlife.
International Federation for Emergency Medicine Consensus Statement: Sonography in hypotension and cardiac arrest (SHoC): An international consensus on the use of point of care ultrasound for undifferentiated hypotension and during cardiac arrest.

Introduction The International Federation for Emergency Medicine (IFEM) Ultrasound Special Interest Group (USIG) was tasked with development of a hierarchical consensus approach to the use of point of care ultrasound (PoCUS) in patients with hypotension and cardiac arrest. METHODS The IFEM USIG invited 24 recognized international leaders in PoCUS from emergency medicine and critical care to form an expert panel to develop the sonography in hypotension and cardiac arrest (SHoC) protocol. The panel was provided with reported disease incidence, along with a list of recommended PoCUS views from previously published protocols and guidelines. Using a modified Delphi methodology the panel was tasked with integrating the disease incidence, their clinical experience and their knowledge of the medical literature to evaluate what role each view should play in the proposed SHoC protocol. RESULTS Consensus on the SHoC protocols for hypotension and cardiac arrest was reached after three rounds of the modified Delphi process. The final SHoC protocol and operator checklist received over 80% consensus approval. The IFEM-approved final protocol, recommend Core, Supplementary, and Additional PoCUS views. SHoC-hypotension core views consist of cardiac, lung, and inferior vena vaca (IVC) views, with supplementary cardiac views, and additional views when clinically indicated. Subxiphoid or parasternal cardiac views, minimizing pauses in chest compressions, are recommended as core views for SHoC-cardiac arrest; supplementary views are lung and IVC, with additional views when clinically indicated. Both protocols recommend use of the "4 F" approach: fluid, form, function, filling. CONCLUSION An international consensus on sonography in hypotension and cardiac arrest is presented. Future prospective validation is required.
C500 variants conveying complete mucosal immunity against fatal infections of pigs with Salmonella enterica serovar Choleraesuis C78-1 or F18+ Shiga toxin-producing Escherichia coli

Salmonella enterica serovar Choleraesuis (S. Choleraesuis) C500 strain is a live, attenuated vaccine strain that has been used in China for over 40 years to prevent piglet paratyphoid. However, this vaccine is limited by its toxicity and does not offer protection against diseases caused by F18+ Shiga toxin-producing Escherichia coli (STEC), which accounts for substantial economic losses in the swine industry. We recently generated a less toxic derivative of C500 strain with both asd and crp deletion (S. Choleraesuis C520) and assessed its efficacy in mice. In addition, we demonstrate that C520 is also less toxic in pigs and is effective in protecting pigs against S. Choleraesuis when administered orally. To develop a vaccine with a broader range of protection, we prepared a variant of C520 (S. Choleraesuis C522), which expresses rSF, a fusion protein comprised of the fimbriae adhesin domain FedF and the Shiga toxin-producing IIe B domain antigen. For comparison, we also prepared a control vector strain (S. Choleraesuis C521). After oral vaccination of pigs, these strains contributed to persistent colonization of the intestinal mucosa and lymphoid tissues and elicited both cytokine expression and humoral immune responses. Furthermore, oral immunization with C522 elicited both S. Choleraesuis and rSF-specific immunoglobulin G (IgG) and IgA antibodies in the sera and gut mucosa, respectively. To further evaluate the feasibility and efficacy of these strains as mucosal delivery vectors via oral vaccination, we evaluated their protective efficacy against fatal infection with S. Choleraesuis C78-1, as well as the F18+ Shiga toxin-producing Escherichia coli field strain Ee, which elicits acute edema disease. C521 conferred complete protection against fatal infection with C78-1; and C522 conferred complete protection against fatal infection with both C78-1 and Ee. Our results suggest that C520, C521, and C522 are competent to provide complete mucosal immune protection against fatal infection with S. Choleraesuis in swine and that C522 equally qualifies as an oral vaccine vector for protection against F18+ Shiga toxin-producing Escherichia coli.
Maternal factors and one-year-olds’ screen time: A cross-sectional study using birth cohort data from the Japan Environment and Children’s Study (JECS)

ABSTRACT Japanese studies have reported that one-year-olds have the longest media exposure among preschoolers. This cross-sectional study assesses associations between the screen time of 12–17-month-old children and their mothers’ characteristics. Using data from the Japan Environment and Children’s Study (JECS), we analyzed 86,938 singletons whose primary caregivers were their mothers. Screen time was defined as time that mothers allowed their children to watch TV, DVDs, and/or other media, categorized into two groups, “less-than-4-hours” and “4-hours-or-more per day. ” The independent variables were children’s sex, mothers’ age, educational attainment, media usage, employment status, frequency of outings, and the existence of caregivers other than the mother. We considered additional factors including scores on the Kessler Psychological Distress Scale ;(K6) and Mother-to-Infant Bonding Scale, Japanese version (MIBS-J). Ninety percent of one-year-olds viewed media content. A multivariate regression analysis revealed that the mother’s media usage was the strongest predictor that a one-year-old would have long screen time (>4 hours/day). A mother’s higher educational attainment and employment status predicted a decreased likelihood of a child’s long screen time. MIBS-J (anger and rejection and impaired bonding) showed very a slight association, and the child’s sex, mother’s age, and K6 had no association with the child’s screen-time. Impact summary Prior State of Knowledge: International guidelines recommend no screen time for one-year-olds. However, Japanese studies have reported that one-year-olds have the longest screen time among preschoolers. Systematic reviews have indicated that a child’s characteristics, family and social context, or ethnicity are factors that influence a child’s screen time. Novel Contributions: This large-scale, Japanese survey targeting only one-year-olds contributes to knowledge of the possible predictors of children’s long screen time. Some findings were similar to previous studies, and some were different. The results differed greatly from international recommendations for the screen time for one-year-olds. Practical Implications: The findings indicate the need for policymakers to develop practical guidelines for promoting rather than prohibiting behavioral changes in the screen time of children. They also suggest the possibility for mothers to reduce the screen time of their children by controlling their own media exposure.
Invisible Users: Youth in the Internet Cafés of Urban Ghana

The urban youth frequenting the Internet cafes of Accra, Ghana, who are decidedly not members of their country's elite, use the Internet largely as a way to orchestrate encounters across distance and amass foreign ties--activities once limited to the wealthy, university-educated classes. The Internet, accessed on second-hand computers (castoffs from the United States and Europe), has become for these youths a means of enacting a more cosmopolitan self. In Invisible Users, Jenna Burrell offers a richly observed account of how these Internet enthusiasts have adopted, and adapted to their own priorities, a technological system that was not designed with them in mind. Burrell describes the material space of the urban Internet cafe and the virtual space of push and pull between young Ghanaians and the foreigners they encounter online; the region's famous 419 scam strategies and the rumors of "big gains" that fuel them; the influential role of churches and theories about how the supernatural operates through the network; and development rhetoric about digital technologies and the future viability of African Internet cafes in the region. Burrell, integrating concepts from science and technology studies and African studies with empirical findings from her own field work in Ghana, captures the interpretive flexibility of technology by users in the margins but also highlights how their invisibility puts limits on their full inclusion into a global network society.
Effect of Dry and Wet Storage on Post Harvest Life and Flower Quality in Cut Tulip cv. Cassini

Experiments were conducted during 2002-03 and 2003-04 to study the influence of storage methods and duration on post harvest quality of cut tulip cv. Cassini. Cut tulips cv. Cassini stored either dry or wet at 4°C for 0,2,4,6 and 8 days showed that days to flower opening was the lowest in those kept under wet storage for 6 and 8 days. Flower opening was better with 0.2 and 4 days of dry or wet storage whereas flowers stored dry for 8 days did not open at all. Flower size and vase life decreased with the increase in storage period. Larger flowers were obtained with dry and wet storage of 0 and 2 days whereas higher vase life was obtained with zero days of wet and dry storage and 4 and 6 days of wet storage.
The search for linguistically coherent accents

Linguistic research refers to many related accents in Southeast England: Standard Southern British English (SSBE), Received Pronunciation (RP), Estuary English (EE), Cockney and Multicultural London English (MLE). However, there is inconsistency and imprecision in the demarcation of these accents based on linguistic and social factors. This paper delineates accents in Southeast England based on patterns of linguistic co-variation which we then relate to social predictors. We applied functional Principal Component Analysis to F1 and F2 measurements for diphthongs extracted from wordlist and passage productions for 193 young, south-eastern speakers. Principal Components were entered into a clustering analysis that identified patterns of linguistic co-occurrence. Three clusters emerge, broadly aligning with SSBE, MLE and EE for both linguistic and social factors. We illustrate the linguistic centre of gravity of the three diphthong systems for use as reference points in future research, and we discuss the need to make explicit how accents are defined.
Maltodextrin as a Drying Adjuvant in the Lyophilization of Tropical Red Fruit Blend

Guava, pitanga and acerola are known for their vitamin content and high levels of bioactive compounds. Thus, the preparation of combinations of these fruits comprises a blend with high nutraceutical potential, yielding a strong and attractive pigmentation material. In this study, the influence of different proportions of maltodextrin on the lyophilization of a blend of guava, acerola and pitanga was evaluated considering not only the physicochemical, physical and colorimetric parameters but also the bioactive compounds in the obtained powders. The blend was formulated from the mixture and homogenization of the three pulps in a ratio of 1:1:1 (m/m), then maltodextrin was added to the blend, resulting in four formulations: blend without adjuvant (BL0), and the others containing 10% (BL10), 20% (BL20) and 30% (BL30) maltodextrin. The formulations were lyophilized and disintegrated to obtain powders. The powders were characterized in terms of water content, water activity, pH, total titratable acidity, ash, total and reducing sugars, ascorbic acid, total phenolic content, flavonoids, anthocyanins, carotenoids, lycopene, color parameters, Hausner factor, Carr index, angle of repose, solubility, wettability and porosity. All evaluated powders showed high levels of bioactive compounds and the increase in maltodextrin concentration promoted positive effects, such as reductions in water content, water activity and porosity and improved flow, cohesiveness and solubility characteristics.
EFFICIENT SEMIPARAMETRIC ESTIMATION OF QUANTILE TREATMENT EFFECTS

This paper develops estimators for quantile treatment effects under the identifying restriction that selection to treatment is based on observable characteristics. Identification is achieved without requiring computation of the conditional quantiles of the potential outcomes. Instead, the identification results for the marginal quantiles lead to an estimation procedure for the quantile treatment effect parameters that has two steps: nonparametric estimation of the propensity score and computation of the difference between the solutions of two separate minimization problems. Root-N consistency, asymptotic normality, and achievement of the semiparametric efficiency bound are shown for that estimator. A consistent estimation procedure for the variance is also presented. Finally, the method developed here is applied to evaluation of a job training program and to a Monte Carlo exercise. Results from the empirical application indicate that the method works relatively well even for a data set with limited overlap between treated and controls in the support of covariates. The Monte Carlo study shows that, for a relatively small sample size, the method produces estimates with good precision and low bias, especially for middle quantiles.
Remarkable response to PD-1 inhibitor in a patient with extensive-stage small cell lung cancer: a case report and literature review

We report a case of a 59-year-old male diagnosed with extensive-stage small cell lung cancer (SCLC). He received first-line platinum doublet chemotherapy and second-line topotecan-based regimen, but experienced disease progression after each line of therapy. He was then treated with Sintilimab, a PD-1 inhibitor, in combination with nab-paclitaxel in the third-line setting, which resulted in significant tumor shrinkage. Restaging scans showed a partial response per RECIST criteria with 62% reduction in tumor burden. This case highlights the application and efficacy of immune checkpoint inhibitors in extensive-stage SCLC.
How did online learning impact the academic performance of graduate students amid the Covid-19 pandemic?: A case study of the United Arab Emirates

Covid-19 has changed the landscape of education forever. Online education has become the new normal for many graduate students with the pandemic outbreak. While online education is more cost-effective, there is little research on how online education has impacted graduate students academically. This study aimed to assess graduate students' academic experiences who took all their courses online. A total of 240 master's and Ph.D. students participated in a questionnaire survey and shared their thoughts on the significance of online education in their academic learning and achievement. The findings revealed that the students perceived strong positive perceptions in terms of engagement, ease of communication, academic learning, and academic achievement as a result of online education. Exploratory Factor Analysis extracted four factors contributing more than 60% variation to online education's overall impact on students. To conclude, online learning provides greater flexibility and convenience, allowing students to study at their own pace and in their own time. Additionally, online learning can be tailored to the individual needs of each student, providing a more personalized learning experience.
Metabolic Profiling in Tuberous Roots of Ranunculus asiaticus L. as Influenced by Vernalization Procedure

Ranunculus asiaticus L. is an ornamental geophyte. In commercial practice, it is mainly propagated by rehydrated tuberous roots. Vernalization before planting is a common practice to overcome the natural dormancy of tuberous roots; however, little is known about the mechanisms underlying the plant’s response to low temperatures. We investigated the influence of three preparation procedures of tuberous roots, only rehydration (control, C), and rehydration plus vernalization at 3.5 °C for 2 weeks (V2) and for 4 weeks (V4), on plant growth, leaf photosynthesis, flowering, and metabolism in plants of two hybrids, MBO (early flowering, pale orange flower) and MDR (medium earliness, bright orange flower), grown in pots in an unheated greenhouse. We reported the responses observed in the aerial part in a previous article in this journal. In this paper, we show changes in the underground organs in carbohydrate, amino acids, polyphenols, and protein levels throughout the growing cycle in the different plant stages: pre-planting, vegetative growth, and flowering. The metabolic profile revealed that the two hybrids had different responses to the root preparation procedure. In particular, MBO synthesized GABA and alanine after 2 weeks and sucrose after 4 weeks of vernalization. In contrast, MDR was more sensitive to vernalization; in fact, a higher synthesis of polyphenols was observed. However, both hybrids synthesized metabolites that could withstand exposure to low temperatures.
The Relationship Between Perceived Human Resource Management Practices and Turnover-Intention: The Mediating Role of Organizational Attractiveness

This study examines whether employees’ attraction to the organisation mediates the relationship between their perceptions of human resource management (HRM) practices (as a bundle) and their turnover intentions. Hypotheses were developed based on the propositions of social exchange theory, findings of previous research and theory of reasoned action. Survey data were collected from 297 individuals who work in organisations operating in the Hashemite Kingdom of Jordan. Subsequently, the proposed relations were tested. It was found that: 1) perceived HRM practices is negatively and significantly related to turnover-intention; 2) perceived HRM practices is positively and significantly related to organisational attractiveness; and 3) organisational attractiveness is negatively and significantly related to turnover intention and partially mediates the negative relationship between perceived HRM practices and turnover intention. The implications of these findings for line managers and HR practitioners are also discussed in addition to future research directions.
Low-dimensional van der Waals materials for linear-polarization-sensitive photodetection: materials, polarizing strategies and applications

Detecting light from a wealth of physical degrees of freedom (e.g. wavelength, intensity, polarization state, phase, etc) enables the acquirement of more comprehensive information. In the past two decades, low-dimensional van der Waals materials (vdWMs) have established themselves as transformative building blocks toward lensless polarization optoelectronics, which is highly beneficial for optoelectronic system miniaturization. This review provides a comprehensive overview on the recent development of low-dimensional vdWM polarized photodetectors. To begin with, the exploitation of pristine 1D/2D vdWMs with immanent in-plane anisotropy and related heterostructures for filterless polarization-sensitive photodetectors is introduced. Then, we have systematically epitomized the various strategies to induce polarization photosensitivity and enhance the degree of anisotropy for low-dimensional vdWM photodetectors, including quantum tailoring, construction of core–shell structures, rolling engineering, ferroelectric regulation, strain engineering, etc, with emphasis on the fundamental physical principles. Following that, the ingenious optoelectronic applications based on the low-dimensional vdWM polarized photodetectors, including multiplexing optical communications and enhanced-contrast imaging, have been presented. In the end, the current challenges along with the future prospects of this burgeoning research field have been underscored. On the whole, the review depicts a fascinating landscape for the next-generation high-integration multifunctional optoelectronic systems.