Miracle-dz/UniFAR-FTUs huggingface dataset:
ISOLASI PLASMID DAN GEN β-LAKTAMASE DARI Escherichia coli SENSITIF DAN RESISTEN AMOKSILIN- KLAVULANAT

There are many incidents of infectious diseases in Indonesia caused by Escherichia coli which is resistant to amoxycillin and amoxycillin-clavulanate. This research is intend to study frequency of E. coli which is resistant to amoxycillin and amoxycillin-clavulanate and plasmid that carried of gene and gene β-lactamase isolation. This research was conducted through descriptive methods, with E.coli identification from clinical samples. Susceptibility test conducted by disk of amoxycillin (25 ug) and amoxycillin-clavulanate (20/10 ug). The existence of plasmid that’s carried of gene and gene β-lactamase in E. coli which is sensitive or resistance of amoxycillin-clavulanate is detected from 4 clinical isolates. The isolation of plasmid through Queigley and Holmes Methode and isolatioan of gene β-lactamase throught amplification DNA with technique of PCR and nested-PCR. The data was analyzed by descriptive methods. The result showed that the frequency of E. coli is greatest isolated from feces, rectal swab and urine. Frequency of E. coli which is resistant amoxycillin is 48,73 % (134 isolates). The use of amoxycillinclavulanate resulted E.coli which is sensitive amoxycillin-clavulanate is 29,85 % (40 isolates), others 44,78 % (60 isolates) tendency to changes to be sensitive (intermedius) and 25,37 % (34 isolates) still resistance to amoxycillin-clavulanate. E.coli which is sensitive or resistence amoxycillin-clavulanate is produce β-lactamase enzymes because in 4 isolates there is plasmid that carried of gene which produced β-lactamase enzyme caused resistance in E. coli. Conclude : Frequency of E. coli which is resistant amoxycillin is 48,73 % and sensitive with the use of amoxycillin-clavulanate is 74,63 % (susceptibility is increasing). β-Lactamases enzyme is produce by E. coli which is sensitive or resistance amoxycillin-clavulanate and all of the β-lactamase genes is located in plamid. Keywords: E.coli, resistant amoxycillin, sensitive and resistance amoxycillinclavulanate, and β-Lactamase enzyme.
Idiopathic gingival fibromatosis with massive gingival overgrowth: A rare case report

Idiopathic gingival overgrowth can present itself as a part of syndrome or as an isolated entity. This is a case report of a massive gingival enlargement in a 12-year-old female child with nonsyndromic gingival fibromatosis, which was treated by multidisciplinary approach involving surgical and prosthetic rehabilitation and at the same time instilling psychological benefit.
Prevalence of cognitive impairment in elderly population residing in an urban area of Belagavi

Context: Cognitive impairment (CI) is emerging as an important health problem of the elderly population in India. As the impact of this illness at various levels is not well understood, so situation analysis is the need of the hour. Aims: (1) To know the prevalence of CI in elderly population residing in an urban area. (2) To assess the burden of health care among the care givers. Settings and Design: A cross-sectional study was carried out among 770 elderly aged ≥60 years residing in two Urban Health Centres of Belagavi District. Methods and Material: Socio-demographic profile of the participant was collected using a predesigned and pretested questionnaire by personal interview at the participants' residence. To assess the CI and burden of healthcare, Mini Mental State Examination and Zarit Caregiver Burden Scale were used, respectively. Statistical Analysis Used: IBM SPSS version 22 was used. Chi square test and Fisher's Exact test were applied. Results: The overall prevalence of CI was 8.4% in our study. The risk factors noted for CI were advancing age, female sex, unmarried or widow/widower, illiterate, not working presently, staying alone, and poverty. Out of 65 caregivers, 67.7% of them had mild or moderate burden of caring for the cognitively impaired elderly. Conclusions: It is important to assess older people for any CI, when planning geriatric health care. Priority must be given to the older age group and female, as they are more vulnerable to CI.
Runs of Homozygosity Reveal Genome- wide Autozygosity in the Austrian Fleckvieh Cattle

Summary Runs of homozygosity (ROH) are recognized as potential inbreeding measure in studies on humans. Inbreeding coeffi cients derived from ROH (F ROH ) measure proportion of the genome arranged in long homozygous segments and highly correlate with those derived from pedigree (F ped ). From that we assumed that ROH represent an alternative to pedigree inbreeding levels in studies on animals too, because pedigree can be incorrect, incomplete and can not fully explain what happened in meiosis. To confi rm our premise we used pedigree and genotype data from 500 Austrian dual purpose Simmental bulls to determine correlation between F ROH and F ped . ROH were obtained using Fortran 90 soft ware created by the authors. Proportions of genome in ROH were calculated for lengths of ROH of >1, >2, >4, >8 and >16 Mb. Pedigree data were analyzed and inbreeding coeffi cients for complete pedigree (FpedT) and fi ve generations (F ped5 ) were calculated using ENDOG soft ware. We found low F pedT and F ped5 (means of 1.5% and 0.9%) while FROH for segments >1Mb suggested much higher values (9.0%) indicating old inbreeding that can not be traced using pedigree. Th e highest correlations were found between FROH calculated from ROH of length >4Mb and F pedT (0.68) that is consistent with studies on humans. We conclude that inbreeding coeffi cients derived from ROH are useful for measuring levels of inbreeding in cattle, because ROH are not subject to mistakes as pedigrees and calculations made from those.
Prevalence and severity of periodontitis in patients with established rheumatoid arthritis and osteoarthritis

Introduction: Periodontitis is associated with many chronic health conditions including diabetes, cardiovascular disease, and rheumatoid arthritis (RA). RA and periodontitis have similarities in inflammatory mechanism, morphology, and histopathology. Osteoarthritis (OA) is a chronic, multifactorial degenerative disease characterized by the deterioration of cartilage in joints. Objective: The aim of this study was to evaluate the prevalence and severity of periodontal disease in patients with established RA and OA. Materials and Methods: A total of 200 patients reporting to the Department of Orthopaedics, KIMSDU, Karad were included in the study. Patients were divided into two groups: Group 1 that included 100 patients with established RA diagnosed according to American College of Rheumatology (ACR) classification 1987 criteria and Group 2 that comprised 100 patients diagnosed with OA. Demographic profile, medical and dental history, oral hygiene practices, and smoking status of study participants were recorded. Periodontal status of the patients were evaluated using the simplified oral hygiene index (OHI-S), Loe and Silness gingival index (GI), probing pocket depth (PPD), and clinical attachment level (CAL). On the basis of the CAL score periodontitis severity was defined as slight, moderate, and severe. Rheumatoid Factor (RF) and C-reactive protein (CRP) were considered as a serological marker in RA. Serological tests were performed to measure RF and CRP. Periodontal parameters and serological tests were correlated. Results: This study reported 45% severe periodontitis prevalence in RA compared to OA group, which was 33%. Severity of periodontitis is significantly greater in RF positive RA group with mean CAL 5.38 mm compared to RF negative RA group with mean CAL 2.81 mm (P = 0.001). There was moderate positive correlation found between RF titer and severity of periodontitis (r = 0.311). Conclusion: The severity of periodontitis was significantly higher among the patients with established RA as compared to patients with OA. RF positive patients had higher periodontal disease compared to RF negative patients. There was an increase in the mean clinical attachment loss with increase in RF titer.
Study of determinants of immunization status among under-five children attending OPD in a rural health center of the tertiary health care institute

Context: The risk of morbidities from vaccine-preventable diseases is highest in those who experience barriers in accessing immunization services. This spectrum could be affordability, accessibility, lack of awareness about immunization services and their health benefits or other limiting factors. The present study aimed to identify maternal and other determinants of immunization status. Aim: To identify maternal and other determinants of immunization status of under-five children. Settings and Design: An OPD based cross sectional survey. Methods and Materials: Non-randomized sample of 194 children (1-5 years) and mothers attending OPD in study duration were examined and interviewed. Statistical Analysis: Data was analysed with SPSS version 20 applying appropriate tests. Results: Out of 194 children, 128 (65.98%) were completely immunized, 45 (23.2%) were partially immunized and 21 (10.82%) were not immunized. Study showed significant association between immunization status and sex of children (P 0.0004), permanent residence (P 0.00001), birth order (P 0.0007), institutional births (P 0.00001), presence of the immunization card (P 0.00001), distance from centre (P 0.0002), age (P 0.00001) and education of mothers (P 0.0001). Association with Religion was statistically non-significant (P 0.0071). Nearness of immunization centre was the main motivating factor for full immunization, whereas unawareness about the need of immunization was prime cause of partial and non-immunization. Conclusion: Present study depicts low immunization completion rate and highlights determinants associated with it. This could be addressed through strong political commitment, infrastructure development and community outreach to achieve the goal of universal immunization.
Knowledge, attitude, and practice of bariatric surgery among adult Saudi community, Saudi Arabia, 2019

The prevalence of overweight and obesity is higher in developed countries than in developing countries of all ages (data from 2013). This study is aimed at assessing the knowledge and attitudes of people in Saudi Arabia toward bariatric surgery. To do this, the specific objectives sought to determine the satisfaction of people who underwent bariatric surgery and to determine the importance of bariatric surgery among the community. Methods: The study is a cross-sectional, community-based study conducted among the Saudi adult population. The final sample size was 891 respondents from different regions of Saudi Arabia. Data collection was through online questionnaires that consisted of 26 questions to assess the perceived knowledge and attitudes toward obesity and bariatric surgery adopted from Abouhamda (2016). Results: More than 70% of the study participants were aged between 18 and 25 years. There were more males than there were females 76.0% versus 24.0%, while most of the respondents were living single. The proportion of respondents across listed regions of Saudi Arabia significantly varied at a 5% level (P = 0.000). Sixty percent of the study respondents were within the normal weight range (BMI 18.5–25), 9% were marked as obese (BMI >30). Age group was not significantly associated with knowledge of quantization and perceived the knowledge that quantization can cause death (P > 0.05 in both cases). Conclusion: The knowledge about obesity in Saudi Arabia is generally good and most Saudis would rather take the approach of proper diet as opposed to surgery, with the bigger percentage being in the normal range (BMI 18.5–25).
Experimental evidence simultaneously confirms and contests assumptions implicit to otolith microchemistry research

Otolith microchemistry has become an integral fisheries tool, yet the utility of this tool hinges on the assumptions that (i) elements are temporally stable once deposited in the otolith and (ii) the influence of maternal effects are temporally limited. Here we test these assumptions by quantifying through time the chemical signatures from two families of brown trout (Salmo trutta), differing in maternal life history (i.e., mothers were either anadromous or nonanadromous). Concentrations of zinc, strontium, and barium were temporally stable in the core signatures between different aged siblings, but significant differences in the elemental concentrations of magnesium and manganese were detected. The Sr concentration (indicative of maternal influence) on the growing surface of otoliths converged briefly between families at 3–5 weeks post-emergence, but never stabilized during the course of the experiment. Taken together, these results both confirm and call into question assumptions implicit to otolith micr.
The photosynthetic response of eight species of Acer to simulated light regimes from the centre and edges of gaps

1. Seedlings of eight Acer species grown under two light treatments simulating forest gap edge (photosynthetic photon flux density, PPFD=30μmolm–2s–1; R:FR=0·6) and gap centre (PPFD=400μmolm–2s–1; R:FR=1·1) were studied to explore the effect of growth irradiance on transient- and steady-state photosynthesis. 2. The increase in maximum photosynthetic rate (Amax) from gap edge- to gap centre-grown seedlings ranged between 20% in Acer saccharum and 138% in Acer ginnala. Amax differed significantly among species (2·3 to 12·3μmolm–2s–1) but assimilation rates under dim light (30μmolm–2s–1) were more similar (0·6 to 1·4μmolm–2s–1). 3. Significant differences among species in stomatal conductance indicate that species of high photosynthetic performance maintain higher dim light conductance and show a much greater excursion in stomatal opening under saturating light. 4. Lightfleck response based on actual photosynthetic rates was more rapid in gap centre- vs gap edge-grown plants but the latter demonstrated more rapid rates of photosynthetic induction (PI). 5. We suggest that the estimation of photosynthetic induction state is prone to an inverse relationship with maximum photosynthetic rate (Amax) and so may not be directly related to efficiency in sunfleck response. Conversely, higher Amax can result in low PI but is also associated with higher transient rates of assimilation during a lightfleck and, therefore, be equally relevant as induction properties. 6. It appears that leaves with characters such as greater specific leaf mass and area-based leaf N, even within the same irradiance treatment, may confer a carboxylation advantage to sunfleck utilization not reflected in PI. However, whether the total carbon return on such a leaf is better assessed by PI or transient photosynthetic rate remains to be determined.
Postdiagnosis weight change is associated with poorer survival in breast cancer survivors: A prospective population‐based patient cohort study

More women are surviving after breast cancer due to early detection and modern treatment strategies. Body weight also influences survival. We aimed to characterize associations between postdiagnosis weight change and prognosis in postmenopausal long‐term breast cancer survivors. We used data from a prospective population‐based patient cohort study (MARIE) conducted in two geographical regions of Germany. Breast cancer patients diagnosed 50 to 74 years of age with an incident invasive breast cancer or in situ tumor were recruited from 2002 to 2005 and followed up until June 2015. Baseline weight was ascertained at an in‐person interview at recruitment and follow‐up weight was ascertained by telephone interview in 2009. Delayed entry Cox proportional hazards regression was used to assess associations between relative weight change and all‐cause mortality, breast cancer mortality, and recurrence‐free survival. In total, 2216 patients were included. Compared to weight maintenance (within 5%), weight loss >10% increased risk of all‐cause mortality (HR 2.50, 95% CI 1.61, 3.88), breast cancer mortality (HR 3.07, 95% CI 1.69, 5.60) and less so of recurrence‐free survival (HR 1.43, 95% CI 0.87, 2.36). Large weight gain of >10% also increased all‐cause mortality (HR 1.64, 95% CI 1.02, 2.62) and breast cancer mortality (HR 2.25, 95% CI 1.25, 4.04). Weight maintenance for up to 5 years in long‐term breast cancer survivors may help improve survival and prognosis. Postdiagnosis fluctuations in body weight of greater than 10% may lead to increased mortality. Survivors should be recommended to avoid large deviations in body weight from diagnosis onwards to maintain health and prolong life.
A meta‐analysis of anal cancer incidence by risk group: Toward a unified anal cancer risk scale

Certain population groups are known to have higher than average anal cancer risk, namely persons living with HIV (PLHIV), men who have sex with men (MSM), women diagnosed with human papillomavirus (HPV)‐related gynecological precancerous lesions or cancer, solid organ transplant recipients (SOTRs) and patients with autoimmune diseases. Our aim was to provide robust and comparable estimates of anal cancer burden across these groups. Summary incidence rates (IRs), as cases per 100 000 person‐years (py), were calculated by fixed‐effects meta‐analysis. IRs were 85 (95% confidence interval [CI] = 82‐89) for HIV‐positive MSM (n = 7 studies; 2 229 234 py), 32 (95% CI = 30‐35) for non‐MSM male PLHIV (n = 5; 1626 448 py) and 22 (95% CI = 19‐24) for female PLHIV (n = 6; 1 472 123 py), with strong variation by age (eg, from 16.8 < 30 years to 107.5 ≥ 60 years for HIV‐positive MSM). IR was 19 (95% CI = 10‐36) in HIV‐negative MSM (n = 2; 48 135 py). Anal cancer IRs were much higher after diagnosis of vulvar (IR = 48 [95% CI = 38‐61]; n = 4; 145 147 py) than cervical (9 [95% CI = 8‐12]; n = 4; 779 098 py) or vaginal (IR = 10 [95% CI = 3‐30]; n = 4; 32 671) cancer, with equivalent disparity after respective precancerous lesions. IR was 13 (95% CI = 12‐15) in SOTRs (n = 5; 1 946 206 py), reaching 24.5 and 49.6 for males and females >10 years after transplant. Anal cancer IRs were 10 (95% CI = 5‐19), 6 (95% CI = 3‐11) and 3 (95% CI = 2‐4) for systemic lupus erythematosus, ulcerative colitis and Crohn's disease, respectively. In conclusion, a unifying anal cancer risk scale, based upon comprehensive meta‐analysis, can improve prioritization and standardization in anal cancer prevention/research initiatives, which are in their public health infancy.
Intracellular prodrug gene therapy for cancer mediated by tumor cell suicide gene exosomes

Recently, we reported about exosomes possessing messenger RNA (mRNA) of suicide gene secreted from mesenchymal stem/stromal cells (MSCs) engineered to express the suicide gene—fused yeast cytosine deaminase::uracil phosphoribosyltransferase (yCD::UPRT). The yCD::UPRT‐MSC exosomes are internalized by tumor cells and intracellularly convert prodrug 5‐fluorocytosine (5‐FC) to cytotoxic drug 5‐fluorouracil (5‐FU). Human tumor cells with the potential to metastasize release exosomes involved in the creation of a premetastatic niche at the predicted organs. We found that cancer cells stably transduced with yCD::UPRT gene by retrovirus infection released exosomes acting similarly like yCD::UPRT‐MSC exosomes. Different types of tumor cells were transduced with the yCD::UPRT gene. The homogenous cell population of yCD::UPRT‐transduced tumor cells expressed the yCD::UPRT suicide gene and secreted continuously exosomes with suicide gene mRNA in their cargo. All tumor cell suicide gene exosomes upon internalization into the recipient tumor cells induced the cell death by intracellular conversion of 5‐FC to 5‐FU and to 5‐FUMP in a dose‐dependent manner. Most of tumor cell‐derived suicide gene exosomes were tumor tropic, in 5‐FC presence they killed tumor cells but did not inhibit the growth of human skin fibroblast as well as DP‐MSCs. Tumor cell‐derived suicide gene exosomes home to their cells of origin and hold an exciting potential to become innovative specific therapy for tumors and potentially for metastases.
Phylogeny and characterisation of Nannochloropsis oceanica var. sinensis var. nov. (Eustigmatophyceae), a new oleaginous alga from China

Cao S. , Zhang X. , Fan X. , Qiao H. , Liang C. , Xu D. , Mou S. , Wang W. and Ye N. 2013. Phylogeny and characterisation of Nannochloropsis oceanica var. sinensis var. nov. (Eustigmatophyceae), a new oleaginous alga from China. Phycologia 52: 573–577. DOI: 10.2216/13-164.1 Strain QD001, previously identified as Chlorella, was examined using molecular phylogenetic analysis (nuclear-encoded 18S rRNA, ITS, rbcL), ITS2 secondary structure, light and electron microscopy, high-performance liquid chromatography pigment analysis, and fatty acid analysis. The strain was shown to be phylogenetically distinct from all described taxa, and the strain was named Nannochloropsis oceanica var. sinensis var. nov. DNA sequences from the new variety were very similar to GenBank sequences for N. maritima nomen nudum.
Association of immigration background with kidney graft function in a publicly funded health system: a nationwide retrospective cohort study in Italy

The impact of immigration background on kidney graft function (eGFR) is unknown. Italy has a publicly funded health system with universal coverage. Since immigration from non‐European Union (EU) countries beyond Eastern Europe is a recent and extensive phenomenon, Italy is a rather unique setting for studying the effect of immigration status as a socioeconomic and cultural condition. We retrospectively identified all adult deceased donor kidney transplant recipients (KTRs) in Italy (2010–2015) and followed them until death, dialysis or 5‐years post‐transplantation; 6346 were EU‐born, 161 Eastern European‐born, and 490 non‐European‐born. We examined changes in eGFR after 1‐year post‐transplant using multivariable‐adjusted joint longitudinal survival random‐intercept Cox regression. Compared to EU‐born KTRs, in non‐European‐born KTRs the adjusted average yearly eGFR decline was −0.96 ml/min/year (95% confidence interval: −1.48 to −0.45; P < 0.001), whereas it was similar in Eastern European‐born KTRs [+0.02 ml/min/year (−0.77 to +0.81; P = 0.96)]. Adjusted 5‐year transplant survival did not statistically differ between non‐European‐born, Eastern European‐born, and EU‐born. In those surviving beyond 1‐year, it was 91.8% in EU‐born (87.1–96.8), 92.5% in Eastern European‐born (86.1–99.4), and 89.3% in non‐European‐born KTRs (83.0–96.0). This study provides evidence that among EU KTRs, non‐European immigration background is associated with eGFR decline.
Expert advice about therapeutic exercise during pregnancy reduces the symptoms of sacroiliac dysfunction

Abstract Objectives There are growing evidence that exercise improves sacroiliac dysfunction symptoms in pregnant women; but no data about the effect of expert advice regarding this matter. The aim of this study was to assess the effectiveness of expert advice about therapeutic exercise on sacroiliac dysfunction in pregnancy. Methods A total of 500 women with sacroiliac dysfunction diagnosed in pregnancy were randomized in study and control group. Study group has conducted expert advice on therapeutic exercise; while control group continued with their normal lifestyle. Pain intensity by Visual Analog Scale (VAS) and degree of functional disability by Quebec scale were assessed at enrolment and after 3 and 6 weeks. Results Significantly better reduction in pain intensity assessed by VAS (p=0.001) and degree of functional disability assessed by Quebec scale (p=0.001) was noted in study compared to control group. Better results for both outcome measures were obtained if intervention was implemented earlier i.e., in second (p=0.001; p=0.001) compared to third (p=0.005; p=0.001) trimester. Strong positive correlation was found between pain intensity and degree of functional disability in both groups. Conclusions Expert advice on therapeutic exercise is effective in reduction of sacroiliac dysfunction symptoms during pregnancy. Trial registration ACTRN12617000556347