Miracle-dz/UniFAR-FTUs huggingface dataset:
Delivery of Active AKT1 to Human Cells

Protein kinase B (AKT1) is a serine/threonine kinase and central transducer of cell survival pathways. Typical approaches to study AKT1 biology in cells rely on growth factor or insulin stimulation that activates AKT1 via phosphorylation at two key regulatory sites (Thr308, Ser473), yet cell stimulation also activates many other kinases. To produce cells with specific AKT1 activity, we developed a novel system to deliver active AKT1 to human cells. We recently established a method to produce AKT1 phospho-variants from Escherichia coli with programmed phosphorylation. Here, we fused AKT1 with an N-terminal cell penetrating peptide tag derived from the human immunodeficiency virus trans-activator of transcription (TAT) protein. The TAT-tag did not alter AKT1 kinase activity and was necessary and sufficient to rapidly deliver AKT1 protein variants that persisted in human cells for 24 h without the need to use transfection reagents. TAT-pAKT1T308 induced selective phosphorylation of the known AKT1 substrate GSK-3α, but not GSK-3β, and downstream stimulation of the AKT1 pathway as evidenced by phosphorylation of ribosomal protein S6 at Ser240/244. The data demonstrate efficient delivery of AKT1 with programmed phosphorylation to human cells, thus establishing a cell-based model system to investigate signaling that is dependent on AKT1 activity.
Surface wave diffraction by a truncated dielectric slab recessed in a perfectly conducting surface

The diffraction of a TM0 surface wave by a terminated dielectric slab which is flush mounted in a perfectly conducting surface is studied. The incident surface wave gives rise to waves reflected and diffracted by the termination; these reflected and diffracted fields may be expressed in terms of the geometrical theory of diffraction by introducing surface wave reflection and diffraction coefficients which are associated with the termination. In this investigation the surface wave reflection and diffraction coefficients have been deduced from a formally exact solution to this canonical problem. The solution is obtained via a combination of the generalized scattering matrix technique and the Wiener-Hopf procedure. Expressions for the reflection and diffraction coefficients contain integrals which can be evaluated numerically.
Localized surface plasmon resonances in graphene ribbon arrays for sensing of dielectric environment at infrared frequencies

High confinement of surface plasmon polaritons in graphene at infrared frequencies enhances the light-matter interaction and can be used for the sensing of the environment. The considered sensing platform consists of parallel graphene ribbons which enables efficient coupling of an electromagnetic field into localized surface plasmons. Changes in the environment are then detected by measuring the resulting frequency shifts of the plasmonic resonances. It is shown that the graphene ribbons have the sensitivity comparable to the sensitivity of noble metal nanoparticles at visible frequencies, which enable sensing of only several nanometers thick films at wavelengths around ten microns. At the same time, the tunability of graphene plasmons enables a design of broadband substrates for surface enhanced infrared absorption of thin films. By changing the Fermi level in graphene, the plasmonic resonance of graphene ribbons can be adjusted to desired vibrational mode which facilitates detection of multiple absorpti.
New Treatment for the Cognitive and Emotional Deficits Linked with Paclitaxel-Induced Peripheral Neuropathy in Mice

Chemotherapy-provoked peripheral neuropathy and its linked comorbidities severely reduce the quality of a patient’s life. Its therapy is not completely resolved and has become an important clinical challenge. The protective actions of molecular hydrogen (H2) in many neurological disorders have been described, but its effects on memory and the emotional deficits accompanying neuropathic pain induced by chemotherapy remain unknown. In this study, using male mice injected with paclitaxel (PTX), we examined the effects of systemic treatment with hydrogen-rich water (HRW) in: (i) the mechanical and thermal allodynia provoked by PTX and the pathways involved; (ii) the memory deficits, anxiety- and depressive-like behaviors associated with PTX-induced peripheral neuropathy (PIPN); and (iii) the plasticity (p-extracellular signal-regulated protein kinase; p-ERK ½), nociceptive (p-protein kinase B, p-Akt), inflammatory (p-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; p-IKBα), and oxidative (4-hydroxynonenal: 4-HNE) alterations provoked by PIPN in the prefrontal cortex (PFC). The results revealed: (1) the antiallodynic actions of HRW administered at one or two times per day during 7 and 3 consecutive days; (2) the participation of Kv7 potassium channels and the Nrf2-heme oxygenase 1-NAD(P)H: quinone oxidoreductase 1 pathway in the painkiller effects of HRW; (3) the inhibition of memory deficits and the anxiodepressive-like behaviors related with PIPN induced by HRW; and (4) the normalization of p-ERK ½, p-Akt and 4-HNE up-regulation and the activation of antioxidant enzymes produced by this treatment in PFC. This study proposes HRW as a possible effective and safe therapy for PIPN and its associated cognitive and emotional deficits.
Effect of nitrogen and zinc management on growth, yield and economics of bread wheat (Triticum aestivum) varieties

Field experiments were conducted during rabi season of 2016â€“17 and 2017â€“18 at Udaipur (Rajasthan) to study the effect of nitrogen and zinc management on growth, yield and economics of different wheat (Triticum aestivum L.) varieties. The treatments consisted of four wheat varieties, viz. Raj 4120, Raj 4037, Raj 4079 and Raj 4238 in main plots and seven nutrient management treatments, viz. 100% RDN, 100% RDN + ZnSO4 25 kg/ha soil application, 100% RDN + ZnSO4 0.5% foliar spray, 100% RDN + ZnSO4 25 kg/ha soil application + ZnSO4 0.5% foliar spray, 125% RDN + ZnSO4 25 kg/ha soil application, 125% RDN + ZnSO4 0.5% foliar spray and 125% RDN + ZnSO4 25 kg/ha soil application + ZnSO4 0.5% foliar spray in sub plots. The study of different wheat varieties indicated that highest values of growth parameters, yield attributes, grain yield (5707 kg/ha), straw yield (8869 kg/ha) and biological yield (14576 kg/ha), and net returns (` 86255/ha) and B:C ratio (2.36) were recorded with wheat variety Raj 4037. Further, application of treatment 125% RDN + ZnSO4 25 kg/ha soil application + ZnSO4 0.5% foliar spray recorded significantly higher values of growth parameters, yield attributes, grain yield (5681 kg/ha), straw yield (8265 kg/ha) and biological yield (13946 kg/ha), and net returns (` 83230/ha).
Standard models for some commuting multioperators

An analogue to the Sz. -Nagy-Foia5 dilation theory is presented for several commuting operators on a Hilbert space. The dilation theory of Hilbert space contractions, a reference of which is the excellent monograph [9], has not yet found a complete counterpart valid for several (commuting) Hilbert space operators. Apart from the results of this type already contained in [9] and other results of general character (i.e., Arveson's or Stinespring's extension theorem), there are not too many contributions specialized to the multiparameter dilation theory. Among these contributions, we quote the works [7, 3-5, 10], which are closer to our topics. The aim of this paper is to analyse some positivity conditions for commuting multioperators, which ensure the unitary equivalence of these objects to some standard models consisting of backwards multishifts. We emphasize, in particular, a situation that seems to satisfy the requirements of an appropriate extension for the dilation theory of Hilbert space contractions and that exploits the results already obtained in [4, 10]. Our finer methods also imply better statements of some assertions from [10] and provide an answer to Question 4.5 from [5]. Let n > 1 be an integer, and let Zn be the set of all n-tuples of nonnegative integers (i.e., the multi-indices of length n). If a = (a, .I, an) E Zn+, we set, as usual, lal =a,+ + -+a, and a! =a1! For a,ficZ+ wewrite a + f = (a1 + f1j . , an + fin), and a < fi whenever aci < /i (i = 1, . , n). Let H be a Hilbert space, and let 2(H) be the algebra of all bounded linear operators on H. An element T = (T,, . , T,) E 2(H)n such that T,, . , T, mutually commute will be designated as a commuting multioperator (briefly, a c.m.). Let T E Y(H)n be a c.m. We define the operator MT: Y(H) -* J(H) by the formula
Metanalysis of Vaccine Efficacy for Monkeypox According to Clinical Criteria of History Taking, Clinical Features and Laboratory Investigation

Monkeypox virus is DNA virus of Orthopoxvirus. The Monkeypox is zoonosis disease first diagnosed in the Democratic Republic of the Congo in 1970 and originally transmitted from Vertebrate Reservoir. In 1796, Edward Jenner was the first physician achieved vaccine and prove its efficacy through exposing James Phipps to smallpox after immunization. The current study revises the vaccine efficacy and mortality rate of monkeypox. It is mainly transmitted from Squirrels to human and cause mortality rate ranging from 2.7 to 10.1%. Globally, it estimated 4.4% the mortality rate of Monkeypox cases. Based on current review study, the effectiveness of smallpox vaccine in preventing the monkeypox is quite variable due to several selective clinical cases criteria such as history taking and clinical features and laboratory investigation, which modify the statistical result of the vaccine efficacy. The current study clarifies the faults of calculation by eliminating several reasons and estimating the effectiveness of vaccine in the household and find it to be 16.3% whereas total Vaccine efficacy is 0.6%. Using preventive measures are the first line to avoid infection therefore healthcare workers must adhere to infection control precautions. A current study warns scientists to create monkeypox vaccine rather than depending on smallpox vaccine effectiveness due to lacking of current vaccine effectiveness evidence and treatment.
The Potential of Tinospora cordifolia Extracts as Antibacterial Material against Pseudomonas aeruginosa

Mitigation and control of bacterial corrosion must have been prioritized for failure anticipation by corrosion associated with microorganisms. Using synthetic inhibitor for material protection from corrosion have problems because it is not eco-friendly. This work conducted preliminary studies on T. cordifolia stem extracts at various methanol ratios against P. aeruginosa biofilm as a new eco-friendly inhibitor. The T. cordifolia stem was extracted by the maceration method with a different ratio of methanol solvent (100, 75 and 50 %). The bacterial activity was assessed using the dilution method (MTT assay) to determine the minimum inhibitory concentration (MIC). The total phenolic and flavonoid contents were determined using Follin-Ciocalteu and aluminum chloride (AlCl3) colorimetric, respectively. Meanwhile, the structure of the active compounds in the extract was identified by using the liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The yields of T. cordifolia extracts are 9.45, 7.56 and 8.40 % at 100, 75 and 50 % of methanol ratios, respectively. The total phenolic content (TPC) in crude methanol extract of T. cordifolia is 11.20, 9.46 and 6.56 % for 100, 75 and 50 % of methanol ratios. Meanwhile, the flavonoid content (TFC) was obtained is about 5.25, 0.64 and 0.33 % for 100, 75 and 50 % of methanol concentrations, respectively. The T. cordifolia extract has antibacterial activity against P. aeruginosa in all ratios of methanol concentrations. The minimum inhibitory concentration (MIC) of T. cordifolia methanol extract was found at 4096 µg/mL. Methanol solvent concentration with the most active antibacterial activity of the extract was 50 %, followed by 75 and 100 %. The structures of the active compound in the methanolic extract of T. cordifolia are a phenolic group, and it is in the alkaloid derivatives (Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, and Yuanhunine). HIGHLIGHTS The crude methanol extract of Tinospora cordifolia stem has potential against aeruginosa in the marine environment The minimum inhibitory concentration (MIC) was found at 4096 µg/mL of extract in a ratio of 50:50 (v/v) of methanol to water The compound structures in cordifolia methanol extracts are Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, and Yuanhunine GRAPHICAL ABSTRACT
Impurity gettering to secondary defects created by MeV ion implantation in silicon

Impurities in MeV-implanted and annealed silicon may be trapped at interstitial defects near the projected ion range, Rp, and also at vacancy-related defects at approximately Rp/2. We have investigated the temperature dependence of impurity trapping at these secondary defects, which were preformed by annealing at 900 °C. The binding energies of Fe, Ni, and Cu are greater at the vacancy-related defects than at extrinsic dislocation loops. During subsequent processing at temperatures up to 900 °C, the amount of these impurities trapped at Rp/2 increases with decreasing temperature while the amount trapped at Rp decreases, with most of the trapped metals located at Rp/2 in samples processed at temperatures ≲ 700 °C. However, intrinsic oxygen is trapped at both types of defects; this appears to have little effect on the trapping of metallic impurities at extrinsic dislocations, but may inhibit or completely suppress the trapping at vacancy-related defects.
Does employee empowerment moderate the effect of situational and dispositional variables on emotional exhaustion differently? The COVID-19 crisis context

Purpose Building on the conservation of resources (COR) theory, this study aims to investigate employee empowerment’s moderation effect on the relationship of situational (job satisfaction, affective commitment) and dispositional (positive affectivity, emotional intelligence) variables toward the emotional exhaustion of service employees amidst the pandemic. Design/methodology/approach In total, 288 service employees from various sectors in Indonesia participate as the study’s respondents. This study applies a two-stage structural equation modeling approach to test the hypotheses. Findings The results show that employee empowerment moderates situational and dispositional variables differently. While employee empowerment significantly influences situational variables, a different situation is found on dispositional variables, that employee empowerment does not significantly influence these variables. This study’s findings portray the COR theory in practice and clarify the importance of employee empowerment for employees with particular attributions. Research limitations/implications The present study bears four limitations: the cross-sectional design; no exploration of dispositional and situational variables’ antecedents; the findings are limited to the service workers; and lastly, this study only takes Indonesian samples. Practical implications From a practical perspective, this study reveals which type of service employees are responsive to empowerment policy and which are prone to experience emotional exhaustion, particularly during a crisis. Social implications By understanding what factors determine employee empowerment’s effectiveness, managers could maximize the impacts of their empowerment policies. Subsequently, it will create better service deliveries which might benefit the broader societal scope. Originality/value This study contributes to both theoretical and practical understanding. Theoretically, this study adds and promotes using a categorical lens to examine the pattern of interactions between organizations and employees.
The impact of motion induced artifacts in the evaluation of HR-pQCT scans of the scaphoid bone: an assessment of inter- and intraobserver variability and quantitative parameters

Background In-vivo high-resolution peripheral quantitative computed tomography (HR-pQCT) has high potential in scaphoid bone pathologies’ scientific and clinical fields. The manufacturer’s visual grading scale (VGS) classifies motion artifacts and divides scans into five quality grades ranging from grade 1 (good quality) to grade 5 (poor quality). This prospective study aimed to investigate the feasibility of the VGS and the influence of image quality on bone density and microarchitecture parameters for the scaphoid bone. Methods Within one year, twenty-two patients with scaphoid fractures received up to six scans of their fractured and contralateral wrist (each consisting of three stacks) using second-generation HR-pQCT (total 256 scans). Three experienced observers graded each stack following the visual grading system, and inter- and intraobserver variability were assessed. The contralateral uninjured scaphoids were then compared pairwise within each patient to high-quality grade 1 scans to determine the influence of image quality on density and microarchitecture parameters. Results Inter- and intraobserver variability among the three observers significantly revealed fair to moderate agreement, P<0.001 and P<0.05, respectively. Bone volume (BV) fraction tended to increase with poorer image quality but did not exceed four percent. Trabecular bone mineral density (Tb.BMD) decreased with poorer image quality but did not exceed five percent. Trabecular number and trabecular thickness significantly increased by 15.5% and 6.8% at grade five (P<0.001), respectively, and trabecular separation significantly decreased by 13.7% at grade five (P<0.001). Conclusions This study revealed a considerable influence of motion on bone morphometry parameters of the scaphoid. Therefore, high image quality must be a central point in studies focusing on the histomorphometry of small objects. The high inter- and intraobserver variability limit the VGS. Future research may focus on other grading systems or automated techniques leading to more consistent and reproducible results. Currently, the use of microarchitectural analysis should be limited to cases without motion artefacts or, at most low graded motion artefacts.
Numerical solution of Fisher's equation

The accurate space derivative (ASD) method for the numerical treatment of nonlinear partial differential equations has been applied to the solution of Fisher's equation, a nonlinear diffusion equation describing the rate of advance of a new advantageous gene, and which is also related to certain water waves and plasma shock waves described by the Korteweg-de-Vries-Burgers equation. The numerical experiments performed indicate how from a variety of initial conditions, (including a step function, and a wave with local perturbation) the concentration of advantageous gene evolves into the travelling wave of minimal speed. For an initial superspeed wave this evolution depends on the cutting off of the right-hand tail of the wave, which is physically plausible; this condition is necessary for the convergence of the ASD method. Detailed comparisons with an analytic solution for the travelling waves illustrate the striking accuracy of the ASD method for other than very small values of the concentration.
Genetic diversity status of only registered cattle breed of Chhattisgarh-Kosali

Assessment of diversity is essential for germplasm characterization and management. Kosali is the first and only registered breed of Chhattisgarh state which immensely contributes to the local economy of 70% farmers. Genomic microsatellite markers being valuable tool for estimating genetic diversity were selected for exploring existing genetic variability in Kosali cattle population. The standard metrics of genomic diversity detected high variability in this breed. All the loci were polymorphic resulting in 297 alleles. Mean values of observed and expected number of alleles were 11.423±0.877 and 4.989±0.372, respectively. Similarly, higher mean values of observed heterozygosity (0.693±0.031) also corroborated with the allelic diversity. Mean expected heterozygosity (0.765±0.02) under Hardy-Weinberg equilibrium (HWE) was higher than the observed values indicating some deviations from assumptions of the model. It can be attributed to the forces such as inbreeding. In fact a positive FIS value of 0.088±0.038 indicated some heterozygote deficiency in the population. Bottleneck analysis indicated that Kosali cattle have not suffered any population bottleneck event during evolutionary trajectory. This study is first to report the genetic diversity status of Kosali cattle based on microsatellite markers.The results imply the necessity of management programs in order to conserve the existing genetic variation and to avoid any escalation of inbreeding.
On thermomechanical restrictions of continua

The central idea proposed here is that, in entropy–producing processes, a specific choice from among a competing class of constitutive functions can be made so that the state variables evolve in a way that maximizes the rate of entropy production. When attention is restricted to quadratic forms for the rate of entropy production, the assumption leads to results that are fully in keeping with linear phenomenological relations that satisfy the Onsager relations. In other words, the usual linear evolution laws such as Fourier's law of heat conduction, Fick's law, Darcy's law, Newton's law of viscosity, etc., all corroborate this assumption. We clarify the difference between the maximum rate of entropy production criterion that characterizes choices among constitutive relations and the minimum entropy production theorem due to Onsager (1931) that characterizes steady states for special choices of the rate of entropy production. We then show that for other forms of entropy production that are not quadratic for which the Onsager relations and related theorems cannot be applied, we can use the procedure described here to obtain nonlinear laws. We demonstrate by means of an example that even yield–type phenomena can be accommodated within this framework, while they cannot within the framework of Onsager. We also discuss issues concerning constraints, especially in thermoelasticity within the context of our ideas.