Miracle-dz/UniFAR-FTUs huggingface dataset:
Modification of the Prosegment in Understanding its Role in the Folding and Function of PMII

MODIFICATION OF THE PROSEGMENT IN UNDERSTANDING ITS ROLE IN THE FOLDING AND FUNCTION OF PMII Ahmad Haniff Jaafar Advisor: University of Guelph, 2014 Professor R.Y. Yada This thesis explores the folding and activation of plasmepsin II (PMII) and the role of its prosegment (PS) on structure – function. Three different constructs of PMII were designed with PSs of varying lengths: extended PSPMII with a 60 residue PS, truncated PSPMII with a 48 residue PS, and NoProPMII with no PS. Extended and truncated PSPMII produced mature enzyme with similar conformation. NoProPMII mature, however, showed improper folding as indicated by low thermal stability, a more solvent-exposed conformation, an 11-fold reduction in the activity assay, and a lower pepstatin-A requirement for complete inhibition. In addition, the PS length was discovered to affect the activation of PMII. Extended PSPMII produced mature enzyme with an extra two PS residues (+2 PMII mature) whereas truncated PSPMII produced mature enzyme with an extra 12 PS residues (+12 PMII mature). The role of a PS in PMII folding was further investigated by conducting folding kinetic studies on PMII. It was found that the native PMII (Np) did not fold at the lowest free energy, but was kinetically stabilized. Upon unfolding, Np formed a thermodynamically stable, yet inactive refolded state (Rp). Np was characterized to have a slow rate of unfolding and folding, as indicated by large free energy barriers to unfold and fold of 24.50 and 25.12 kcal/mol, respectively. In the presence of the extended PS (60 residues), the energy landscape was shifted and the activation energy barrier was lowered to 12.37 kcal/mol, which enhanced the folding rate by approximately 18,550 times. To elucidate the effect of PS residue length on PMII folding, structural analysis and in silico simulation were conducted on the two zymogens of PMII: extended PSPMII and truncated PSPMII. Both zymogens appeared to be in a more solvent-exposed conformation as compared to their mature forms. Due to the oppositely charged residues between the main protein body and the PS, it appeared that the interactions between both structures were driven by electrostatic forces with 27 and 34 interactions (H-bonds and salt-bridges) measured between the main PMII body and truncated and extended PS, respectively.
Negative Career Thoughts Among Pakistani and Korean College Students: Implications for the Asian Context

This study aimed at investigating the integrative cross-cultural perspective of negative career thoughts by comparing college students’ data from Pakistan and Korea. Data obtained from 200 Pakistani college students were compared to the data obtained from 200 Korean college students. Results show a significantly higher score on negative career thoughts when compared to Korean students. Findings indicate cross-cultural differences in negative career thoughts reflective of context-based differences in policy and career guidance systems. Findings provide support to Systems Theory Framework. However, the similarities in the construct manifestation provide a theoretical basis for career guidance in the collectivist and Asian contexts. Findings suggest the importance of comparative data, indigenization of assessment tool to assess negative career thoughts, and further exploration of negative career thoughts while considering cultural dimensions.
Middle Devonian uncinuloids (Brachiopoda, Rhynchonellida) from North Africa and Central Europe

The paper includes a taxonomic revision of four externally similar Middle Devonian rhynchonellide species from northwestern Africa (Maider, Tindouf Syncline) and Central Europe (Eifel, Bergisches Land, Holy Cross Mts.), considered in recent papers as representatives of Kransia Westbroek, 1967 or Nalivkinaria Rzhonsnitskaya, 1968. All four possess a septalium and a multilamellate cardinal process, the assignment to Nalivkinaria , having a bifid cardinal process, is therefore clearly inappropriate. Lebanzuella ? issoumourensis (Drot, 1971) is present in the Givetian of Africa; two subspecies, L. issoumourensis issoumourensis from Jbel Issoumour and L. issoumourensis smarensis ssp. nov. from Western Sahara, are distinguished by their biometric characteristics. The other two species are included in Kransia ( Fatimaerhynchia ) subgen. nov. differing from Kransia ( K. ) in the presence of a septalium; the occurrence of such a variable structure is considered to be justification for distinction at the subgenus level. Kransia ( Fatimaerhynchia ) goldfussii (Schnur, 1853) is an Eifelian species. Kransia ( Fatimaerhynchia ? ) aff. goldfussii from the Givetian of Bilveringsen is a separate species (larger, more transverse, more strongly ornamented), which is not described because of insufficient material. Kransia ( Fatimaerhynchia ) signata (Schnur, 1851) is present in the Middle Devonian of Jbel Issoumour, the middle Eifelian of the Eifel and the (upper? ) Givetian of the Holy Cross Mountains.
Precise fabrication of single-atom alloy co-catalyst with optimal charge state for enhanced photocatalysis

Abstract While the surface charge state of co-catalysts plays a critical role for boosting photocatalysis, studies on surface charge regulation via their precise structure control remain extremely rare. Herein, metal-organic framework (MOF) stabilized bimetallic Pd@Pt nanoparticles, which feature adjustable Pt coordination environment and a controlled structure from core-shell to single-atom alloy (SAA), have been fabricated. Significantly, apart from the formation of a Mott-Schottky junction in a conventional way, we elucidate that Pt surface charge regulation can be alternatively achieved by changing its coordination environment and the structure of the Pd@Pt co-catalyst, where the charge between Pd and Pt is redistributed. As a result, the optimized Pd10@Pt1/MOF composite, which involves an unprecedented SAA co-catalyst, exhibits exceptionally high photocatalytic hydrogen production activity, far surpassing its corresponding counterparts.
Prevalence of Cytomegalovirus and its Roles in Cytokines Stimulation in Immunocompromised Patients in Mosul City, Iraq

Cytomegaloviruses (CMV) cause different infections in humans all over the world with a prevalence exceeds 70% in adults and 90% in poorer communities and developing countries. Two hundred immunocompromised patients (cancer, dialysis, thyroid gland, and thalassemia) infected with CMV were subjected to determine CMV seroprevalence and its effect on cytokines expression using advance molecular test (Real time PCR) and Serological ELISA test. The results showed high CMV seroprevalence rate in immunocompromised patients but in low rate in thalassemia patients in Mosul city compared with other cities near Iraq. CMV stimulates upregulation in expression (mRNA transcripts) of three cytokines genes (IL-10, TNF, and CCL2) which affect four key immune pathways which in turn may cause severe complication on patients such as asthma and cytokine storm.
Molecular Electrostatic Potential of the Main Monoterpenoids Compounds Found in Oil Lemon Tahiti (Citrus Latifolia Var Tahiti)

The work is a study of the geometry of the molecules via molecular mechanics of the main monoterpenoids found in the oil of Lemon Tahiti. Lemon Tahiti is the result of grafting of Persia file on Rangpur lime and has no seeds. A computational study of the molecular geometry of the molecules through molecular mechanics of the main monoterpenoids compounds present in fruit oil is described in a computer simulation. The fruit has active terpenoids compounds: alfa-pinene, beta-pinene, limonene and gama-terpenine. The studied monotepernoides form two groups of distribution characteristics of fillers and similar electrical potentials between groups. Since alfa-pinene and limonene present, major and minor moment of electric dipoles, respectively.
Errors by Simpson and Dervin (2019) in their description of the Council of Europe’s Reference Framework of Competences for Democratic Culture

In a recent paper, Simpson and Dervin (2019a) offer a radical critique of the Council of Europe’s Reference Framework of Competences for Democratic Culture (RFCDC). However, Simpson and Dervin’s paper contains numerous factual errors, interpretative errors and category errors in its description of the RFCDC. We identify 12 such errors which invalidate the conclusions drawn by them. We correct all of these errors, and suggest that, rather than using Simpson and Dervin’s paper as a source of information about the RFCDC, readers should read the RFCDC itself, before drawing their own conclusions about the RFCDC and the adequacy of the arguments offered by Simpson and Dervin.
Extinct and extant Pacific Trogossitidae and the evolution of Cleroidea (Coleoptera) after the Late Triassic biotic crisis

A new subgenus of Tenebroides, Polynesibroides subgen. nov., is established for six potentially extinct beetles, Tenebroides atiu, T. moorea, T. raivavae, T. rimatara, T. tubuai and T. mihiura spp. nov., described from the Cook, Austral and Society Islands based on subfossil fragments, and the extant T. tahiti sp. nov. from the island of Tahiti, Society Islands. Here we present the first detailed time-scaled phylogeny of the superfamily Cleroidea inferred from our four-gene dataset, including T. tahiti. Bayesian tip-dating, incorporating 17 fossils, estimated that Cleroidea appeared at the end of the Triassic (~210 Mya). The split of Biphyllidae + Byturidae + Phloiophilidae was reconstructed at ~204 Mya, followed by Early Jurassic separation of Trogossitidae (~200 Mya), Acanthocnemidae (~197 Mya), stem Cleridae–Thanerocleridae–Chaetosomatidae (~194 Mya), Peltidae, Lophocateridae, Decamerinae and the melyrid lineage (~191 Mya). Trogossitidae diversified by separation of Kolibacia (~136 Mya) in the Cretaceous, followed by parting Temnoscheila + Nemozoma and Airora + Tenebroides lineages (~113 Mya). Tenebroides radiated ~75 Mya and T. tahiti diverged from Panamanian taxa in the Middle Eocene (~40 Mya). Fourteen morphological characters of Pacific trogossitids were analysed. Parallelodera, including the Panamanian species, is nested within Tenebroides being sister to Polynesibroides. Therefore, Parallelodera is classified as a subgenus of Tenebroides. Tenebroides fairmairei is placed in the subgenus Parallelodera. A Neotropical origin for Parallelodera and Polynesibroides is inferred.
Pengaruh Citra Merek Terhadap Loyalitas Konsumen

Abstract— The purpose of this research is to find out how the company efforts in building the brand image, and to find out costumer loyalty, also how much the influence of the brand image to costumer loyalty. These types of research are conducted verification using the Insidental Sampling data collection technique by using a sample of 100 consumer respondents of Ultra Teh Kotak in Bandung City. The data of analysis used to test the hypothesis is regression analysis. These results of this research indicate that the implementation of the layout in Ultra Teh Kotak is strong, means that the brand image has a positive significant influence to costumer loyalty. Keywords— brand image, costumer loyalty Abstrak— Tujuan penelitian ini adalah untuk mengetahui bagaimana upaya perusahaan dalam membangun citra merek, bagaimana loyalitas konsumen minmuan teh dalam kemasan merek Ultra Teh Kotak dan seberapa besar pengaruh citra merek terhadap konsumen. Jenis penelitian yang dilaksanakan ini bersifat verifikatif dengan menggunakan teknik pengumpulan data Insidental Sampling dengan menggunakan 100 sampel responden konsumen Ultra Teh Kotak di Kota Bandung. Analisis data yang digunakan untuk menguji hipotesis adalah analisis regresi. Hasil penelitian ini menunjukan bahwa pelaksanaan citra merek yang dilakukan Ultra Teh Kotak tergolong kuat, artinya citra merek berpengaruh berpengaruh signifikan terhadap loyalita konsumen. Kata Kunci—Citra merek, loyalitas konsumen
Combined Impact of Pre-sensitization and Delayed Graft Function on Allograft Rejection in Deceased Donor Kidney Transplantation: Nationwide Cohort Study

The aim of this study is to investigate whether or not delayed graft function (DGF) and pre-transplant sensitization has a synergistic adverse effect on allograft outcome after deceased donor kidney transplantation (DDKT) using the Korean Organ Transplantation Registry (KOTRY) database, the nationwide prospective cohort. The study included 1,370 cases between May 2014 and June 2019. The cases were divided into 4 subgroups according to pre-sensitization and the development of DGF post-transplant (non-pre-sensitized-DGF(-)(n=1100), non-pre-sensitized-DGF(+)(n=133), pre-sensitized-DGF(-)(n=116), and pre-sensitized-DGF(+)(n=21)). We compared the incidence of biopsy-proven allograft rejection (BPAR), time-related change in allograft function, allograft or patient survival, and post-transplant complications across 4 subgroups. The incidence of overall BPAR and acute antibody-mediated rejection (ABMR) was significantly higher in the pre-sensitized-DGF(+) subgroup than in other 3 subgroups. In addition, multivariable cox regression analysis demonstrated that pre-sensitization combined with DGF is an independent risk factor for both overall BPAR (hazard ratio 3.253, p = 0.005) and acute ABMR (hazard ratio 7.589, p < 0.001). Moreover, DGF and pre-sensitization showed significant interaction. Pre-sensitization combined with DGF did not show significant impact on allograft function, and allograft or patient survival. In conclusion, pre-sensitization and DGF had a synergistic adverse impact on allograft rejection after DDKT.
Effect of red koji as a Starter Culture in “Wanergao”: A Traditional Fermented Food in China

Abstract The objective of this study was to explore the effect of red kojis on essential indices of Wanergao. The results showed that red koji‐inoculated Wanergao showed higher pH values (4.38 ± 0.06 and 4.39 ± 0.06) and lower TA values (1.61 ± 0.05 and 1.63 ± 0.05) compared to the control group. LAB and yeast in the starter culture group gradually increased to 7.57 ± 0.12, 7.64 ± 0.15 log cfu.g−1 and 8.59 ± 0.21, 8.64 ± 0.23 log cfu.g−1, respectively. During fermentation, the dominant microorganism was Lactobacillus plantarum and Saccharomyces cerevisiae. Compared to the Wanergao made using traditional backslopping, the red koji‐inoculated Wanergao contained more amylases, EAA and DAA contents compared to the control sample. The red kojis and control samples presented different hardness, chewiness, and cohesiveness, as well as similar values in springiness, gumminess, and adhesiveness. Sensory analysis also showed higher chewiness aroma and resilience of Wanergao in the starter culture group than in the control group.
Analysis of mtDNA control region of an isolated population of Eld’s deer (Rucervus eldii) reveals its vulnerability to inbreeding

Abstract The Eld’s deer or brow-antlered deer (Rucervus eldii) is one of the most endangered cervids of Southeast Asia. Geographically, it has three distinct subspecies; Sangai or Manipur's brow-antlered deer (R. e. eldii), Siamese brow-antlered deer (R. e. siamensis) and Thamin or Myanmar’s brow antlered deer (R. e. thamin). We examined the genetic diversity of wild and captive populations of R. e. eldii and compared its relationship with other subspecies using mtDNA control region gene. During the analysis, only one haplotype was detected in 30 samples of R. e. eldii. No genetic variation was observed among the R. e. eldii populations. The reduced genetic diversity indicates that the population has passed through the bottleneck effect that might have resulted in the inbreeding depression.
Proposing a new model for shopping centre attractiveness assessment by a Combination of Structural Equation Modelling (SEM) and Additive Ratio ASsessment (ARAS)

ABSTRACT This research paper presents a new framework by combined usage of Structural Equation Modelling (SEM) and Additive Ratio ASsessment (ARAS) for assessing shopping centre attractiveness. This study is organized in two phases. In the first phase, aiming to develop attractiveness of shopping centres in tourism destinations, and based on a sequential steps procedure, a comprehensive list of shopping centre attractiveness measures was compiled from literature review, and in a two-step survey, research model is developed by SEM, including five dimensions of (1) appearance, (2) internal atmosphere, (3) products, (4) human interactions and, (5) shopping convenience. At the end of this phase, the weight of each dimension was calculated based on high order factor loadings derived from SEM findings. In the second phase, by use of ARAS, the attractiveness of six shopping centres was assessed and ranked, based on the extracted dimensions. This article methodologically takes contribution from a combination of SEM-ARAS, while SEM was used for causal relationships and assigning weights for the ARAS input. The shopping centre attractiveness measurement provides a reliable model for local, regional and national authorities to boost shopping tourism in tourism destinations.
New remains of Cephalomys arcidens (Rodentia, Caviomorpha) and a redefinition of the enigmatic Cephalomyidae

Cephalomys is the best-known cephalomyid, recorded in five localities in Argentina and known from well-preserved mandibles, fragments of skull, and a large number of isolated teeth, from both juveniles and adults. Despite this, a modern description is lacking and the validity of some species of Cephalomys has been questioned in the last few years. New material from the Deseadan locality of Cabeza Blanca (Chubut, Argentina), including skull fragments unknown for the species thus far, and well-preserved juvenile teeth, along with a large number of specimens from collections in Argentina, allow us to propose a more complete description of the genus and a new taxonomic arrangement for the Cephalomys species. We also analysed some dubious records of Cephalomys from Quebrada Honda and Pico Truncado and confirm that they are not part of this genus. We consider C. plexus to be a synonym of C. arcidens, we consider the combination previously proposed for C. bolivianus (as a species of Asteromys) to be valid, and we consider C. ceciae also to be a valid species. We performed a phylogenetic analysis including for the first time all cephalomyids (Cephalomys, Litodontomys, Cephalomyopsis, Soriamys and Banderomys) and possibly related genera (Perimys, Scotamys, Asteromys) to test the position of the family Cephalomyidae among the other caviomorph rodents, as well as its validity and internal relationships. The results recovered Cephalomyidae as a clade, including Cephalomys, Cephalomyopsis and Litodontomys. The monophyly of Cephalomys could not be confirmed. Soriamys and Banderomys were recovered within Cavioidea. Perimys, Scotamys and Asteromys were recovered within Chinchilloidea, and we analysed the implications of their positions within the group. The implications in terms of the biochron, distribution and evolutionary history of the Cephalomyidae, considering the new definition for the family proposed herein (including only Cephalomys, Cephalomyopsis and Litodontomys), are discussed, as well as inferences regarding caviomorph evolution at the scale of the entire group.
Validation of a Synthetic-Eddy Method for Modelling Incoming Wakes in Scale-Resolving Simulations of Turbomachinery Cascades

An enhanced Synthetic-Eddy Method for the modelling of free stream turbulence and incoming wakes for scale-resolving simulations of turbomachinery cascades is validated. It is shown that a previously presented method produces valid and physically plausible wakes representative of turbomachinery, and correctly reproduces their influence on the downstream cascade. The validation test case is an experimentally extensively investigated low-pressure turbine cascade with bars moving in pitch direction upstream of the cascade. Validation takes place in two steps: First, only the bars are considered. Then the profile including the moving bars is evaluated. In addition to the simulations with the directly modelled incoming wakes via the new method, the upstream wakes of the above two configurations are also directly resolved. The validation is proven by comparing the experimental values with the results of simulations with directly modelled wakes and the simulations with meshed moving bars. INTRODUCTION The wakes caused by the rotation of a turbomachine and the high turbulence levels outside the wake flow typical of turbomachines have a significant influence on the transition behaviour and thus also the losses of a turbomachine. The type of inflow therefore should be considered in the aerodynamic design of a turbomachine. According to the current state of the technology, turbomachinery currently is aerodynamically designed using computeraided simulation methods known as Unsteady Reynolds Average Navier-Stokes Equations (URANS). A major disadvantage of these methods, however, is their modelling of turbulence. While current RANS methods have high prediction accuracies for steady inflows, Müller et al. (2015) could show which deficits current URANS methods have with regard to transient inflows, hence the current focus on this topic. Due to their modelling, URANS procedures require extensive calibrations. Testing and calibration of URANS methods is currently carried out using experimentally investigated cascades. However, the generation of engine-like wakes upstream of the cascade to be investigated is difficult in the experiment. Either the Strouhal numbers (Stadtmüller et al., 2000) of current experimental investigations are clearly below those in a real engine, or a significant influence of the measurement setup cannot be excluded (Berrino et al., 2014). Due to the steady decrease of the cost of computational resources, however, it is now possible to carry out so-called scaleresolving simulations (SRS) such as Large Eddy Simulation (LES) for technically relevant flows. In order to achieve signifiThis work is licensed under Attribution 4.0 International (CC BY 4.0) See:https://creativecommons.org/licenses/by/4.0/legalcode Table 1 Geometric parameters of the validation test case
A quasi-experimental study on the influence of different media scaffolds toward physics problem-solving processes

ABSTRACT Physics is a subject requiring strong logical thinking, and writing a problem-solving path according to logical thinking can enhance students’ knowledge and problem-solving ability. The purpose of the study was to explore the effects of problem presentation of different scaffolds (MAPS or dynamic figures) on students’ physical problem solving. MAPS scaffold is a modeling applied to problem-solving pedagogy. The study adopts a quasi-experimental design, as participants were divided into four groups tested under different scaffolds. These groups are Traditional group (problems with static figures), MAPS group (MAPS combined with static figures), Dynamic Figure group (problems with dynamic figures), and MAPS + Dynamic Figure group (MAPS combined with dynamic figures). The results indicated that the students assisted by MAPS could more effectively describe and interpret key concepts and knowledge in questions, and they selected formulas appropriately according to the physics conditions. However, the findings did not reveal any significant difference between the dynamic figure. Thus, we will explore dynamic figures in different types of physics questions in future research, so that dynamic figures can be more effectively used by students for physics problem-solving.