Miracle-dz/UniFAR-FTUs huggingface dataset:
DNA methylation alterations in systemic lupus erythematosus: A systematic review of case-control studies

Background Traditionally, the diagnosis and monitoring of disease activity in systemic lupus erythematosus (SLE) are contingent upon clinical manifestations and serological markers. However, researchers are struggling to find biomarkers with higher sensitivity and specificity. DNA methylation has been the most studied epigenetic feature in SLE. So, in this study, we performed a systematic review of studies about DNA methylation alterations in SLE patients compared to healthy controls. Methods By searching PubMed, Scopus, and Google Scholar up to July 2022, all case-control studies in which DNA methylation of specific genes was assessed by a non-high-throughput technique and passed the quality of bias assessment were included. Results In total, 44 eligible studies underwent a data extraction process. In all, 3471 SLE patients and 1028 healthy individuals were included. Among the studies that reported the patients’ gender (n = 2853), 89.41% were female and 10.59% were male. Forty studies have been conducted on adult patients. The number of works on fractionated and unfractionated blood cells was almost equal. In this regard, 22 studies were conducted on whole blood or peripheral blood mononuclear cells and two studies on unfractionated white blood cells. Sorted blood cells were biological sources in 20 studies. The most investigated gene was IFI44L. Sensitivity, specificity, and diagnostic power of methylation levels were only reported for IFI44L in five studies. The most employed methylation profiling method was bisulfite sequencing polymerase chain reaction. The correlation between methylation patterns and clinical parameters was explored in 22 studies, which of them 16 publications displayed a remarkable association between DNA methylation status and clinical indices. Conclusions The methylation status of some genes especially IFI44L, FOXP3, and MX1 has been suggested as promising SLE biomarkers. However, given the conflicting findings between studies because of potential confounders such as different sample types, methylation profiling methods, and ethnicity as well as shared DNA methylation patterns of SLE and other autoimmune diseases, DNA methylation biomarkers are currently not reliable diagnostic biomarkers and do not represent surrogate markers of SLE disease activity. Future investigations on a larger scale with the discarding of limitations of previous studies would probably lead to a consensus.
Effects of 8-Week Electromyostimulation Training on Upper-Limb Muscle Activity and Respiratory Gas Analysis in Athletes with Disabilities

This study was aimed at verifying the efficacy of EMS training by investigating the changes in upper-limb muscle functions and energy expenditure in athletes with disabilities after an 8-week intervention of EMS training. We compared variations in muscle activity, respiratory gas, and symmetry index (SI) after an 8-week intervention in eight professional male athletes with disabilities wearing an electromyostimulation (EMS) suit (age: 42.00 ± 8.67 years, height: 1.65 ± 0.16 m, weight: 64.00 ± 8.72 kg, career length: 11.75 ± 3.83 years). For EMS training, each participant wore an EMS suit. EMS was applied to the upper-limb muscles pectoralis major and triceps at 40 °C water temperature, with a 25 Hz frequency (duty cycle 10%) for 15 min, followed by a 5 Hz frequency (duty cycle 5%) for 5 min. The pre- and post-intervention measurements were taken in the same way at a pre-set time (for 1 h, twice a week) for 8 weeks. Training involved a seated chest press, and the muscle activity (pectoralis major, triceps, and antebrachial muscles), upper-limb SI, and respiratory gas variables (maximal oxygen consumption (VO2), carbon dioxide output (VCO2), respiratory quotient (RQ), metabolic equivalents (METs), and energy expenditure per min (Energy expended per minute; EEm)) were analyzed. Variations pre- and post-intervention across the measured variables were analyzed. Regarding the change in muscle activity, significant variations were found in the pectoralis major right (p < 0.004), pectoralis major left (p < 0.001), triceps right (p < 0.002), and antebrachial right (p < 0.001). Regarding left-to-right SI, a positive change was detected in the pectoralis major and triceps muscles. Additionally, respiratory gas analysis indicated significant variations in VO2 (p < 0.001), VCO2 (p < 0.001), METs (p < 0.001), and EEm (p < 0.001). EMS training improved muscle strength and respiratory gas variables and is predicted to contribute to enhanced muscle function and rehabilitation training for athletes with disabilities.
Sustainability Report Disclosure: Analysis of the impact of company characteristics and Good Corporate Governance

The value relevance of accounting information, including sustainability reports, is attractive to companies because of its impact on the company's competitiveness. Analyzing the potential factors that have an impact on the disclosure of sustainability reports can provide additional insight for stakeholders about the transparency of corporate actions to mitigate impacts and risks. Sustainability reporting in times of crisis due to the pandemic will be more important than ever to increase trust among all stakeholders. The purpose of this study is to obtain empirical evidence of the effect of company characteristics and good corporate governance on the disclosure of sustainability reports. The type of research used in this study is quantitative research that emphasizes theory testing through measuring research variables with numbers and analyzing data with statistical procedures for multiple linear regression analysis which is done with Eviews 10. The results of the study empirically prove that company size affects sustainability report disclosures while profitability, liquidity, audit committee, and the board of directors do not affect the sustainability report disclosure.
The FDA-Approved Drug Pyrvinium Selectively Targets ER+ Breast Cancer Cells with High INPP4B Expression

Simple Summary Wnt/β-catenin signaling is hyperactivated in many human cancers including up to 50% of breast cancers. Although there has been significant progress in developing therapeutics that suppress Wnt/β-catenin signaling, particularly in colon cancer, the repurposing of FDA-approved therapeutics is likely to be a faster and more cost-effective method to target this pathway in human disease. Pyrvinium is an FDA-approved anthelmintic drug used to treat pinworms, which also suppresses Wnt/β-catenin signaling by activating the β-catenin destruction complex protein, CK1α. Here, we demonstrate that breast cancer cells with increased expression of the oncogene INPP4B, a PI3K regulator that promotes Wnt/β-catenin activation, are selectively sensitive to pyrvinium treatment in 2D and 3D culture. Therefore, Wnt inhibition using pyrvinium may be an effective strategy for treating human breast cancers with high INPP4B expression. Abstract The majority of breast cancers are estrogen receptor-positive (ER+), and endocrine therapies that suppress ER signaling are the standard-of-care treatment for this subset. However, up to half of all ER+ cancers eventually relapse, highlighting a need for improved clinical therapies. The phosphoinositide phosphatase, INPP4B, is overexpressed in almost half of all ER+ breast cancers, and promotes Wnt/β-catenin signaling, cell proliferation and tumor growth. Here, using cell viability assays, we report that INPP4B overexpression does not affect the sensitivity of ER+ breast cancer cells to standard-of-care treatments including the anti-estrogen 4-hydroxytamoxifen (4-OHT) or the PI3Kα inhibitor alpelisib. Examination of four small molecule Wnt inhibitors revealed that ER+ breast cancer cells with INPP4B overexpression were more sensitive to the FDA-approved drug pyrvinium and a 4-OHT-pyrvinium combination treatment. Using 3D culture models, we demonstrated that pyrvinium selectively reduced the size of INPP4B-overexpressing ER+ breast cancer spheroids in the presence and absence of 4-OHT. These findings suggest that repurposing pyrvinium as a Wnt inhibitor may be an effective therapeutic strategy for human ER+ breast cancers with high INPP4B levels.
Colitis with Hypereosinophilia following the Second Dose of the BNT162b2 mRNA COVID-19 Vaccine: A Case Report with a Literature Review

A 61-year-old man presented with a 7-day history of watery diarrhea and loss of appetite after receiving the second dose of the Pfizer-BioNTech COVID-19 vaccine. Laboratory studies showed significant eosinophilia and an elevated IgE level (white cell count, 18.4×109/L; eosinophil count, 9.5×109/L; and IgE level, 540 IU/L). Symptoms resolved 10 days after vaccination without any steroids or antiallergic medications, and the eosinophil count had also returned to within normal limits 2 months later. Several cases of eosinophilic disorders following receipt of any type of injectable COVID-19 vaccine have been reported, so the etiology should be examined.
The increasingly blurred line between induction, consolidation and maintenance in acute myeloid leukaemia

Since the early 1970s, the treatment of acute myeloid leukaemia (AML) has undergone a major transformation. Initially based on only two drugs, an anthracycline and cytosine arabinoside, the aim of therapy was to achieve a haematological response allowing patients to recover and go home. Back in those early days, cure was not a realistic expectation. Treatment was analogous to a heart attack; upon recovery and a short respite, recurrence and death inevitably followed. Over the subsequent decades, slow but remarkable progress was made such that a subgroup of young adults could become long‐term survivors. This astonishing feat was achieved initially without the use of new drugs. Supportive care played a major role with the widespread availability of platelet transfusions and improved antimicrobial therapy, particularly antifungal. No less important was the better use of existing drugs and the development of allogeneic haematopoietic cell transplantation. While initially the focus was on maximal tolerated therapy, an understanding of the immunologic role of allogeneic transplantation, better genetic characterization of the biology of the disease, advanced tools for detection of minimal disease as well as the recent development of new drugs changed the focus to a more refined approach targeting patients who are more likely to respond. Clearly, the historical paradigm where the term AML was generic and applicable to all patients requires a rethinking from the traditional therapeutic demarcations of therapy into phases of induction, consolidation and maintenance. These evolving new concepts and paradigm will be herein considered.
Microstructure and Solute Segregation around the Melt-Pool Boundary of Orientation-Controlled 316L Austenitic Stainless Steel Produced by Laser Powder Bed Fusion

For this article, we studied the microstructure and solute segregation seen around the melt pool boundary of orientation-controlled 316L austenitic stainless steel produced by laser powder bed fusion, using transmission electron microscopy and energy-dispersive x-ray spectroscopy. We found that the solidification cellular microstructures could be visualized with the aid of solute segregation (Cr and Mo) during solidification. Mn–Si–O inclusions (10–15 nm in diameter) were distributed along the lamellar boundaries, as well as in the dislocation cell walls. It is believed that the grain growth of the inclusions can be effectively suppressed by rapid quenching during the laser powder-bed fusion process. A thin region without cellular microstructures was observed at the melt-pool boundary. The cellular spacing widened near the bottom of the melt-pool boundary, owing to the decrease in the cooling rate. Atomic-structure analysis at the lamellar boundary by high-resolution transmission electron microscopy revealed a local interfacial structure, which is complementary to the results of electron back-scatter diffraction.
Open-ended movements structure sensorimotor information in early human development

Significance It is postulated that the various human behaviors, consisting structured sensorimotor interactions, are established based on experiences from the early developmental stage. From this perspective, spontaneous whole-body movements, which neonates and infants specifically exhibit without an explicit purpose like “sensorimotor wandering,” could contribute to the structuration of the sensorimotor interactions later observed in task-specific movements, such as walking or reaching. In this research, we identified both spatial and temporal structures in muscular sensorimotor interactions under spontaneous movements and their developmental changes during early infancy. These findings highlight the contribution of early open-ended movements to the self-organized structuring of sensorimotor interactions. Moreover, they further provide conceptual insight that links early whole-body movements to spontaneous neuronal activity maturing in an autonomous fashion.
Relationship between Hormonal Changes and Self-Perceived Halitosis in Females: A Cross-Sectional Study

Halitosis is a growing concern for patients seeking dental treatment. Women experience hormonal changes throughout different phases of their life. These changes may affect volatile organic compound (VOC) production and can be linked to halitosis. The aim of this study was to evaluate the effect of hormonal changes on self-perceived halitosis in a sample of females using a self-administered questionnaire. This study used a cross-sectional observational design. The questionnaire was distributed electronically through Google forms. A total of 1089 participants completed the questionnaire. Almost 50% of participants were below the age of 25; and 12% were of menopausal age (defined as 45 years and older). Most participants reported having regular menstrual cycles (63.5%) while only 20% reported having hormonal disturbances. Almost 62% of the sample reported that they notice halitosis on themselves with 27.6% indicating their perception of halitosis occurred at different times of the month. Around 12% of the sample thought that a relationship between halitosis and their menstrual cycle existed with 4.6% reporting halitosis during menstruation. The present study found that regularity of menstrual cycle and hormonal disturbances can affect females’ self-perceived halitosis and the prevalence of oral health symptoms.
But who trains the language teacher educator who trains the language teacher? An empirical investigation of Chilean EFL teacher educators’ language assessment literacy

Research has shown that language teachers typically feel underprepared for assessment aspects of their job. One reason may relate to how teacher education programmes prepare future teachers in this area. Research insights into how and to what extent teacher educators train future language teachers in language assessment matters are scarce, however, as are insights into the language assessment literacy (LAL) of the teacher educators themselves. Additionally, while increasingly research insights are available on components that constitute LAL, how such components interrelate is largely unexplored. To help address these research gaps, we investigated the LAL of English as a Foreign Language teacher educators in Chile. Through interviews with 20 teacher educators and analysis of their language assessment materials, five LAL components were identified (language assessment knowledge, conceptions, context, practices, and learning), and two by-products of LAL (language assessor identity and self-efficacy). The components were found to interrelate in a complex manner, which we visualized with a model of concentric oval shapes, depicting how LAL is socially constructed (and re-constructed) from and for the specific context in which teacher educators’ practices are immersed. We discuss implications for LAL conceptualisations and for LAL research methodology.
Gravitational instability on propagation of MHD waves in astrophysical plasma

Abstract We determine the general dispersion relation for the propagation of magnetohydrodynamic (MHD) waves in astrophysical plasma by considering the effect of gravitational instability and viscosity with anisotropic pressure tensor and heat-conducting plasma. Basic MHD equations have been derived and linearized by the method of perturbation to develop the general form of dispersion relation equation. Our result indicates that the transverse propagation of waves in such a plasma is affected by the inclusion of heat conduction. For wave propagation, parallel to the magnetic field direction, we find that the fairhose mode is unaffected, whereas the mode corresponding to the gravitational instability is modified in astrophysical plasma with anisotropic pressure tensor being stable in the presence of viscosity and strong magnetic field at considerable wavelength.
Emotional Solidarity and Co-creation of Experience as Determinants of Environmentally Responsible Behavior: A Stimulus-Organism-Response Theory Perspective

Effective approaches to mitigate the adverse environmental impacts of tourism development have become one of the most prevalent topics in tourism. However, limited research analyzes the role of social interactions in tourists’ environmentally responsible behavior. This study is one of the first to investigate two distinct antecedents representing visitors’ interactions with service providers and residents to explain tourists’ behavior. Drawing on the Stimulus-Organism-Response theory, a mixed-methods sequential explanatory research design was employed by collecting quantitative data through a survey and following up with qualitative data from in-depth interviews. Based on a survey of 441 respondents, the study confirmed the positive effects of co-creation experiences between tourists and service providers, as well as emotional solidarity between visitors and residents, on tourists’ environmentally responsible behavior at destinations. An analysis of 10 in-depth interviews reveals new important factors contributing to tourists’ environmentally responsible behavior and its outcomes.
Cost‐saving population genomic investigation of Daphnia longispina complex resting eggs using whole‐genome amplification and pre‐sequencing screening

Abstract Resting stages of aquatic organisms that accumulate in the sediment over time are an exceptional resource that allows direct insights into past populations and addressing evolutionary questions. This is of particular interest in taxa that face relatively new environmental challenges, e.g., climate change and eutrophication, such as the Daphnia longispina species complex, a keystone zooplankton group in European freshwater ecosystems. However, genomic analysis might be challenging as DNA yield from many of these resting stages can be low and the material degraded. To reliably allow the resequencing of single Daphnia resting eggs from different sediment layers and characterize genomic changes through time, we performed whole‐genome amplification to obtain DNA amounts suitable for genome resequencing and tested multiple protocols involving egg isolation, whole‐genome amplification kits, and library preparation. A pre‐sequencing contamination screening was developed, consisting of amplifying mitochondrial Daphnia and bacterial markers, to quickly assess and exclude possibly contaminated samples. In total, we successfully amplified and sequenced nine genomes from Daphnia resting eggs that could be identified as Daphnia longispina species. We analyzed the genome coverage and heterozygosity of these samples to optimize this method for future projects involving population genomic investigation of the resting egg bank.
Antimicrobial Properties of Different Hop (Humulus lupulus) Genotypes

The antimicrobial activity of hop extracts obtained from different hop genotypes were investigated against Staphylococcus aureus and Lactobacillus acidophilus. In this study the pure xanthohumol, purified β-acids rich fraction, as well as α-acids with β-acids rich fraction were used to test antimicrobial activity against Staphylococcus aureus and Lactobacillus acidophilus; whereby, the antimicrobial activity of different hop extracts against Lactobacillus acidophilus was studied for the first time. Microbial susceptibility to purified hydroacetonic extracts from different hop varieties was investigated by the broth microdilution assay to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The hop hydroacetonic extracts were more effective against Staphylococcus aureus than against Lactobacillus acidophilus. Strong inverse correlations of MIC and MBC values were obtained with xanthohumol, cohumulone, n+adhumulone, colupulone and n+adlupulone contents, suggesting that the identified chemical hop compounds are directly responsible for antimicrobial effects. Moreover, the effect of the growth medium strength on the MIC values of hop extracts against Staphylococcus aureus was systematically investigated for the first time. The current study also reveals the effect of different hop extracts on Staphylococcus aureus, which responds to their presence by lag phase extension and generation time prolongation.
Parametric studies on traveling compression regions observed in the Earth’s magnetotail

On the basis of the spontaneous fast reconnection model, the traveling compression region (TCR) is studied by magnetohydrodynamic simulations for various plasma parameters. Once the fast reconnection mechanism involving slow shocks builds up, the general features of TCR, observed by virtual satellites located in the simulation box, are in good agreement with actual satellite observations. Quantitatively, the TCR signature is not significantly influenced by plasma β, whereas its duration time is proportional to VAe−1, where VAe is the Alfven velocity in the magnetic field region; also, the compression ratio of TCR is larger for the smaller plasma density ρe in the magnetic field region because of larger compressibility. In the diffusion region, resistive tearing is likely to occur, giving rise to multiple small-scale TCRs following the major TCR. For the uniform resistivity model, where the fast reconnection mechanism is not realized, any TCR signature cannot be observed. Hence, the TCR signature, observed.
Beyond a platform protein for the degradosome assembly: The Apoptosis-Inducing Factor as an efficient nuclease involved in chromatinolysis

Abstract The Apoptosis-Inducing Factor (AIF) is a moonlighting flavoenzyme involved in the assembly of mitochondrial respiratory complexes in healthy cells, but also able to trigger DNA cleavage and parthanatos. Upon apoptotic-stimuli, AIF redistributes from the mitochondria to the nucleus, where upon association with other proteins such as endonuclease CypA and histone H2AX, it is proposed to organize a DNA–degradosome complex. In this work, we provide evidence for the molecular assembly of this complex as well as for the cooperative effects among its protein components to degrade genomic DNA into large fragments. We have also uncovered that AIF has nuclease activity that is stimulated in the presence of either Mg2+ or Ca2+. Such activity allows AIF by itself and in cooperation with CypA to efficiently degrade genomic DNA. Finally, we have identified TopIB and DEK motifs in AIF as responsible for its nuclease activity. These new findings point, for the first time, to AIF as a nuclease able to digest nuclear dsDNA in dying cells, improving our understanding of its role in promoting apoptosis and opening paths for the development of new therapeutic strategies.