Miracle-dz/UniFAR-FTUs huggingface dataset:
The compound effect of irradiation and familial pseudohyperkalemia on potassium leak from red blood cells

Familial pseudohyperkalemia (FP) is a rare asymptomatic condition characterized by an increased rate of potassium leak from red blood cells (RBC) on refrigeration. Gamma irradiation compromises RBC membrane integrity and accelerates potassium leakage. Here, we compared the effect of irradiation, applied early or late in storage, on FP versus non‐FP RBC.
Genomic Epidemiology of Vibrio cholerae O139, Zhejiang Province, China, 1994–2018

Cholera caused by Vibrio cholerae O139 was first reported in Bangladesh and India in 1992. To determine the genomic epidemiology and origins of O139 in China, we sequenced 104 O139 isolates collected from Zhejiang Province, China, during 1994–2018 and compared them with 57 O139 genomes from other countries in Asia. Most Zhejiang isolates fell into 3 clusters (C1–C3), which probably originated in India (C1) and Thailand (C2 and C3) during the early 1990s. Different clusters harbored different antimicrobial resistance genes and IncA/C plasmids. The integrative and conjugative elements carried by Zhejiang isolates were of a new type, differing from ICEVchInd4 and SXTMO10 by single-nucleotide polymorphisms and presence of genes. Quinolone resistance–conferring mutations S85L in parC and S83I in gyrA occurred in 71.2% of the Zhejiang isolates. The ctxB copy number differed among the 3 clusters. Our findings provided new insights for prevention and control of O139 cholera .
A SARM1-mitochondrial feedback loop drives neuropathogenesis in a Charcot-Marie-Tooth disease type 2A rat model

Charcot-Marie-Tooth disease type 2A (CMT2A) is an axonal neuropathy caused by mutations in the mitofusin 2 (MFN2) gene. MFN2 mutations result in profound mitochondrial abnormalities, but the mechanism underlying the axonal pathology is unknown. Sterile α and Toll/IL-1 receptor motif–containing 1 (SARM1), the central executioner of axon degeneration, can induce neuropathy and is activated by dysfunctional mitochondria. We tested the role of SARM1 in a rat model carrying a dominant CMT2A mutation (Mfn2H361Y) that exhibits progressive dying-back axonal degeneration, neuromuscular junction (NMJ) abnormalities, muscle atrophy, and mitochondrial abnormalities — all hallmarks of the human disease. We generated Sarm1-KO (Sarm1–/–) and Mfn2H361Y Sarm1 double-mutant rats and found that deletion of Sarm1 rescued axonal, synaptic, muscle, and functional phenotypes, demonstrating that SARM1 was responsible for much of the neuropathology in this model. Despite the presence of mutant MFN2 protein in these double-mutant rats, loss of SARM1 also dramatically suppressed many mitochondrial defects, including the number, size, and cristae density defects of synaptic mitochondria. This surprising finding indicates that dysfunctional mitochondria activated SARM1 and that activated SARM1 fed back on mitochondria to exacerbate the mitochondrial pathology. As such, this work identifies SARM1 inhibition as a therapeutic candidate for the treatment of CMT2A and other neurodegenerative diseases with prominent mitochondrial pathology.
Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy

Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential to regulate multiple signaling pathways in target cells. This potential is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated as important and have been associated with a wide variety of EV-induced biological phenomena. While controlled loading of single miRNAs is a well-documented approach for enhancing EV bioactivity, loading of multiple miRNAs has not been fully leveraged to maximize the potential of EV-based therapies. Here, an established approach to extrinsic nucleic acid loading of EVs, sonication, was utilized to load multiple miRNAs in HEK293T EVs. Combinations of miRNAs were compared to single miRNAs with respect to anti-inflammatory outcomes in assays of increasing stringency, with the combination of miR-146a, miR-155, and miR-223 found to have the most potential amongst the tested groups.
Quantifying the burden of the post-ICU syndrome in South Africa: A scoping review of evidence from the public health sector

Background The post-ICU syndrome (PICS) comprises unexpected impairments in physical, cognitive, and mental health after intensive care unit (ICU) discharge, and is associated with a diminished health-related quality of life (HRQOL). A Cochrane review recommended more research in this field from low- and middle-income countries. Objectives This review aims to examine the extent and nature of publications in the field of PICS in the South African (SA) public health sector. Findings of available local research are contextualised through comparison with international data. Methods A comprehensive literature search strategy was employed. Inclusion criteria comprised publications enrolling adult patients following admission to SA public hospital ICUs, with the aim to study the main elements of PICS (ICU-acquired neuromuscular weakness, neurocognitive impairment, psychopathology and HRQOL). Results Three studies investigated physical impairment, 1 study psychopathology, and 2 studies HRQOL. Recommended assessment tools were utilised. High rates of attrition were reported. Neuromuscular weakness in shorter-stay patients had recovered at 3 months. Patients who were ventilated for ≥5 days were more likely to be impaired at 6 months. The study on psychopathology reported high morbidity. The HRQOL of survivors was diminished, particularly in patients ventilated for ≥5 days. Conclusion This review found a paucity of literature evaluating PICS in the SA public health sector. The findings mirror those from international studies. Knowledge gaps pertaining to PICS in medical, surgical and HIV-positive patients in SA are evident. No publications on neurocognitive impairment or the co-occurrence of PICS elements were identified. There is considerable scope for further research in this field in SA. Contributions of the study This review identified the available publications investigating the post ICU syndrome (PICS) in the South African public healthcare setting. A small number of ground-breaking studies were found. Knowledge gaps in this field were identified.
Reliable change indices for the Italian Edinburgh Cognitive and Behavioral ALS Screen (ECAS)

Abstract This study aimed at providing standardized regression-based (SRB) reliable change indices (RCIs) for the Italian Edinburgh Cognitive and Behavioral ALS Screen (ECAS). Thirty-one consecutive ALS patients undergoing the ECAS were followed-up (T1) at 6.5 ± 1 months (range = 5–8). Ceiling/floor effects, practice effect, and test–retest reliability were assessed. Each ECAS measure was regressed by stepwise-entering as predictors demographics, respective T0 scores, T0 disease duration and ALSFRS-R, retest interval, and progression rate (ΔFS) – i.e., (48 - ALSFRS-RT0)/disease durationT0 in months. Ceiling effects were infrequently detected, no practice effect emerged and all ECAS measures were reliable at retest (except for Language and Visuo-spatial subscales). T0 scores predicted all ECAS measures except for the Visuo-spatial subscale. The availability of RCIs for the Italian ECAS will aid ALS-related clinical practice and research within the longitudinal dimension.
Dark energy in Horndeski theories after GW170817: A review

The gravitational wave (GW) event GW170817 from a binary neutron star merger together with the electromagnetic counterpart showed that the speed of GWs [Formula: see text] is very close to that of light for the redshift [Formula: see text]. This places tight constraints on dark energy models constructed in the framework of modified gravitational theories. We review models of the late-time cosmic acceleration in scalar–tensor theories with second-order equations of motion (dubbed Horndeski theories) by paying particular attention to the evolution of dark energy equation of state and observables relevant to the cosmic growth history. We provide a gauge-ready formulation of scalar perturbations in full Horndeski theories and estimate observables associated with the evolution of large-scale structures, cosmic microwave background and weak lensing by employing a so-called quasi-static approximation for the modes deep inside the sound horizon. In light of the recent observational bound of [Formula: see text], we also classify surviving dark energy models into four classes depending on different structure-formation patterns and discuss how they can be observationally distinguished from each other. In particular, the nonminimally coupled theories in which the scalar field [Formula: see text] has a coupling with the Ricci scalar [Formula: see text] of the form [Formula: see text], including [Formula: see text] gravity, can be tightly constrained not only from the cosmic expansion and growth histories but also from the variation of screened gravitational couplings. The cross-correlation of integrated Sachs–Wolfe signal with galaxy distributions can be a key observable for placing bounds on the relative ratio of cubic Galileon density to total dark energy density. The dawn of GW astronomy will open up a new window to constrain nonminimally coupled theories further by the modified luminosity distance of tensor perturbations.
Alterations in facial expressions in individuals at risk for psychosis: a facial electromyography approach using emotionally evocative film clips

Abstract Background Negative symptoms such as blunted facial expressivity are characteristic of schizophrenia. However, it is not well-understood if and what abnormalities are present in individuals at clinical high-risk (CHR) for psychosis. Methods This experimental study employed facial electromyography (left zygomaticus major and left corrugator supercilia) in a sample of CHR individuals (N = 34) and healthy controls (N = 32) to detect alterations in facial expressions in response to emotionally evocative film clips and to determine links with symptoms. Results Findings revealed that the CHR group showed facial blunting manifested in reduced zygomatic activity in response to an excitement (but not amusement, fear, or sadness) film clip compared to controls. Reductions in zygomatic activity in the CHR group emerged in response to the emotionally evocative peak period of the excitement film clip. Lower zygomaticus activity during the excitement clip was related to anxiety while lower rates of change in zygomatic activity during the excitement video clip were related to higher psychosis risk conversion scores. Conclusions Together, these findings inform vulnerability/disease driving mechanisms and biomarker and treatment development.
Entrainment model for fully-developed wind farms: Effects of atmospheric stability and an ideal limit for wind farm performance

While a theoretical limit has long been established for the performance of a single turbine, no corresponding upper bound exists for the power output from a large wind farm, making it difficult to evaluate the available potential for further performance gains. Here we build a model describing the essential features of a large array of turbines with arbitrary design and layout, by considering a fully-developed wind farm whose upper edge is bounded by a self-similar boundary layer. The exchanges between the wind farm, the overlaying boundary layer, and the outer flow are parameterized by means of the classical entrainment hypothesis. We obtain a concise expression for the wind farm's power density (corresponding to power output per unit planform area), as a function of three coefficients, which represent the array thrust and the turbulent exchanges at each of the two interfaces. We assess the performance of our model by comparing the predicted power density to field data, laboratory measurements and large-eddy simulations for the fully-developed regions of wind farms, finding good agreement. We extend our model to include the effect of atmospheric stability on power output, by using an established parameterization relating entrainment coefficients to local Froude numbers. Our predictions for power variation with atmospheric stability are in broad agreement with field measurements. To the best of our knowledge, this constitutes the first quantitative comparison between an atmospheric-stability-dependent model and field data. Finally, we consider an ideal limit for array operation, whereby turbines are designed to maximize momentum exchange with the overlying boundary layer. This enables us to obtain an upper bound for the performance of large wind farms, which we determine to be an order of magnitude larger than the output of contemporary turbine arrays.
Improving accessing to perinatal mental health care

Perinatal mental health difficulties affect 1 in 5 women during pregnancy and after birth. They can have a negative impact on women and their families and are estimated to cost the UK up to £8.1 billion for every annual cohort of women giving birth (Bauer et al., 2014). The evidence suggests not all women who experience perinatal mental health difficulties access the care they need (Maternal Mental Health Alliance, 2022a), so a critical question is how best to ensure women with perinatal mental health difficulties access perinatal mental health care. In this editorial we consider the findings of a recent evidence synthesis programme, the MATRIx programme (Perinatal Mental Health Assessment and TReatment: An Evidence Synthesis and Conceptual Framework of Barriers and Facilitators to Implementation) which synthesised evidence from 46 empirical studies, 32 systematic reviews, and stakeholder consultations to identify key barriers and facilitators to women accessing perinatal mental health care (Webb et al., Submitteda, 2021). Sixty-six barriers and 39 facilitators were identified across the care pathway. These arose at multiple levels (e.g. individual, organisational, political) and were summarised into two conceptual frameworks that provide pictorial representations of these barriers and facilitators (Webb et al., Submittedb) From this work the MATRIx programme developed evidence-based recommendations for national health service policy, practice, and research. The implementation of these recommendations could have a positive impact on ensuring women with perinatal mental health difficulties access the care they need. Recommendations were developed for women, health professionals, service managers, commissioners, and the government. We outline some of these briefly below before considering possible ways these might be implemented in practice. Recommendations for health professionals include having high quality training on perinatal mental health, listening to women’s concerns, and providing assessment in a woman-centred way. Communication with other health professionals also needs to be clear and open in order to prevent women falling through the gaps between services. Recommendations for service managers focus on workforce, workplace culture and practice, and service provision. The workforce needs to have an adequate number of staff to meet women’s needs, and preferably staff with a positive interest in providing high quality physical and psychological care to women. The evidence synthesis results suggested barriers to care are reduced if health professionals are from a similar background to women, therefore services should aim to recruit a diverse workforce. High quality, accredited training requires appropriate funding and protected time, and should be matched to workforce training needs and competencies (Health Education England, 2018). JOURNAL OF REPRODUCTIVE AND INFANT PSYCHOLOGY 2022, VOL. 40, NO. 5, 435–438 https://doi.org/10.1080/02646838.2022. 2121993
Surgical treatment of endometrioid endometrial carcinoma - laparotomy versus laparoscopy

Objective: Recent publications have raised doubts about the oncological safety of a laparoscopic approach in the treatment of endometrial cancer. The aim of this study was to investigate the beneficial aspects of laparoscopy versus laparotomy in patients with endometrial cancer, and present oncological outcomes. Material and Methods: A retrospective study of patients who underwent surgery for the treatment of endometrioid endometrial cancer was performed. Surgical outcomes and complications in patients who were treated by laparoscopy or open surgery were compared. The patients were followed for 5-years. Patients’ characteristics, tumor stage, complications rate and oncologic outcome were analyzed. Results: A total of 151 patients were included. The laparoscopy (n=80) and laparotomy (n=71) groups were homogeneous in regards of demographic data and tumor stage. Median average blood loss (1.31 vs. 1.92 g/dL), the mean duration of hospitalization (5.73 vs. 12.25 days), intraoperative (0 vs. 6%), and severe postoperative complications (5.1 vs. 14.3%) were significantly lower in the laparoscopy group. The numbers of pelvic or para-aortic lymph nodes removed during systematic lymphadenectomy were similar in both groups. Women who underwent laparoscopy and those who underwent laparotomy had similar five-year recurrence-free survival rates (88.7% vs. 91.5%, p=0.864), as well as similar overall five-year survival rates (91.2% vs. 97.2%, p=0.094). Conclusion: The oncological outcome of laparoscopy was similar to that of laparotomy in the treatment of patients with endometrial cancer. However, surgical outcomes and morbidity rates were significantly better in patients treated by laparoscopy. Clinical trials are essential to evaluate the oncological efficacy of laparoscopy in patients with endometrial cancer.
Microbial-induced Redox Imbalance in the Neonatal Lung Is Ameliorated by Live Biotherapeutics

Bronchopulmonary dysplasia (BPD) is a common lung disease of premature infants. Hyperoxia exposure and microbial dysbiosis are contributors to BPD development. However, the mechanisms linking pulmonary microbial dysbiosis to worsening lung injury are unknown. Nrf2 (nuclear factor erythroid 2-related factor 2) is a transcription factor that regulates oxidative stress responses and modulates hyperoxia-induced lung injury. We hypothesized that airway dysbiosis would attenuate Nrf2-dependent antioxidant function, resulting in a more severe phenotype of BPD. Here, we show that preterm infants with a Gammaproteobacteria-predominant dysbiosis have increased endotoxin in tracheal aspirates, and mice monocolonized with the representative Gammaproteobacteria Escherichia coli show increased tissue damage compared with germ-free (GF) control mice. Furthermore, we show Nrf2-deficient mice have worse lung structure and function after exposure to hyperoxia when the airway microbiome is augmented with E. coli. To confirm the disease-initiating potential of airway dysbiosis, we developed a novel humanized mouse model by colonizing GF mice with tracheal aspirates from human infants with or without severe BPD, producing gnotobiotic mice with BPD-associated and non–BPD-associated lung microbiomes. After hyperoxia exposure, BPD-associated mice demonstrated a more severe BPD phenotype and increased expression of Nrf2-regulated genes, compared with GF and non–BPD-associated mice. Furthermore, augmenting Nrf2-mediated antioxidant activity by supporting colonization with Lactobacillus species improved dysbiotic-augmented lung injury. Our results demonstrate that a lack of protective pulmonary microbiome signature attenuates an Nrf2-mediated antioxidant response, which is augmented by a respiratory probiotic blend. We anticipate antioxidant pathways will be major targets of future microbiome-based therapeutics for respiratory disease.
The association between parents' stress and parental feeding practices and feeding styles: Systematic review and meta‐analysis of observational studies

Abstract In the extended UNICEF framework of early childhood nutrition, parents' stress is associated with parental feeding style. However, no comprehensive review has examined the association between parents' stress and feeding styles and practices. The objective of our review was to synthesise the current literature examining the association between parents' stress and their feeding practices and/or styles, among parents of children ≤ 5 years old. We searched; MEDLINE, EMBASE, PSYCHINFO and CINAHL from 2019 to 2021. Two investigators independently extracted relevant data and assessed the study quality and the certainty of evidence. Data were pooled using generic inverse variance with fixed effects (<5 comparisons) or random effects (≥5 comparisons) and expressed as correlation coefficients with 95% confidence intervals (CI). Between study heterogeneity was assessed using Cochran's Q and quantified with I 2. We identified 6 longitudinal and 11 cross‐sectional studies, of which 4 studies provided sufficient data to be pooled. A very small correlation between general stress and restrictive feeding practices was observed (r = 0.06 [95% CI: 0.01−0.12]; no substantial heterogeneity (I 2 = 0.00%, P Q < 0.85, very low certainty). No correlation between general stress and feeding pressure was identified (r = 0.06 [95% CI: −0.02 to 0.15]). Results showed that both general and parenting stress were associated with suboptimal breastfeeding practices and unresponsive feeding styles. Conclusion: This study demonstrated a low‐to‐moderate quality of literature for the inclusion of parents' stress in the extended UNICEF care model of child nutrition. Future research needs to explore this relationship longitudinally and in ethnic diverse populations to inform tailored interventions that promote responsive feeding practices.
On the Anomalous Temperature Distribution of the Intergalactic Medium in the NGC 3411 Group of Galaxies

We present XMM-Newton, Chandra, and VLA observations of the USGC S152 group and its central elliptical NGC 3411. Imaging of the group X-ray halo suggests that it is relaxed with little apparent structure. We investigate the temperature and metal abundance structure of the group halo and find that while the abundance distribution is fairly typical, the temperature profile is highly unusual, showing a hot inner core surrounded by a cool shell of gas with a radius of ~20-40 kpc, at the center of the larger group halo. Spectral mapping confirms an irregular ring of gas ~0.15 keV cooler than its surroundings. We estimate the total mass, entropy, and cooling time profiles within ~200 kpc, and find that the cool shell contains ~9 × 109 M☉ of gas. VLA observations at 1.4, 5, and 8 GHz reveal a relatively weak nuclear radio source, with a core radio luminosity LR = 2.7 × 1038 ergs s-1 and a diffuse component extended on scales of a few arcseconds (or more). A lack of evidence for activity at optical or X-ray wavelengths supports the conclusion that the central black hole is currently in a quiescent state. We discuss possible mechanisms for the formation of temperature features observed in the halo, including a previous period of AGN activity, and settling of material stripped from the halo of one of the other group member galaxies.