Miracle-dz/UniFAR-FTUs huggingface dataset:
The Mechanisms of Sodium Chloride Stress Mitigation by Salt-Tolerant Plant Growth Promoting Rhizobacteria in Wheat

We investigated the growth-promoting mechanism of salt-tolerant plant growth promoting rhizobacteria (ST-PGPR) in wheat under sodium chloride (NaCl) stress by measuring the growth and physiological and biochemical responses of wheat plants inoculated with ST-PGPR under 0–400 mM NaCl. The results showed that ST-PGPR plays a significant role in the growth of wheat under NaCl stress. Under 300 mM NaCl, wheat plants inoculated with the three ST-PGPR strains increased in plant height, root length, dry weight, and fresh weight by 71.21%, 89.19%, 140.94%, and 36.31%, respectively, compared to the control group. The proline and soluble sugar contents of wheat inoculated with Bacillus thuringiensis increased by 38.8% and 21.4%, respectively. The average content of antioxidant enzymes increased by 13.89%, and compared with the control, in wheat inoculated with the three species of ST-PGPR, the average content of ethylene decreased 2.16-fold. In addition, a mathematical model based on the “interaction equation” revealed that the best results of mixed inoculation were due to the complementary strengths of the strains. The analysis of experimental phenomena and data revealed the mechanisms by which Brevibacterium frigoritolerans, Bacillus thuringiensis, and Bacillus velezensis alleviate NaCl stress in wheat: (1) by lowering of osmotic stress, oxidative stress, and ethylene stress in wheat and (2) by using root secretions to provide substances needed for wheat. This study provides a new approach for the comprehensive understanding and evaluation of ST-PGPR as a biological inoculant for crops under salt stress.
Insight into fermentable sugar recovery process from sugarcane bagasse: in silico elucidation of enzymatic hydrolysis and techno-economic assessment

A technological scheme was developed to produce 2G fermentable sugar from sugarcane bagasse and was assessed for the process feasibility. This paper describes recovery of fermentable sugar, characterization of pretreated hydrolysed bagasse, in-silico elucidation of enzymatic hydrolysis and techno-economic analysis of the recovery of sugar from bagasse. The in-silico approach included characterization of both the enzymes, estimation of physiochemical and functional properties through ProtParam. Secondary structures of enzymes were represented using SOPMA. Modelling of enzymes was done using MODELLER 9.24. The developed model was validated through PROCHECK and was evaluated using Verify3D. The active sites of both the enzymes were predicted using 3D LigandSite – Ligand binding site prediction server and molecular docking of the predicted Models were performed using Swiss Dock. The techno-economic analysis showed 0.516 kg (2G fermentable sugar)/kg bagasse). GRAPHICAL ABSTRACT
Modeling Secondary Phenotypes Conditional on Genotypes in Case–Control Studies

Traditional case–control genetic association studies examine relationships between case–control status and one or more covariates. It is becoming increasingly common to study secondary phenotypes and their association with the original covariates. The Orofacial Pain: Prospective Evaluation and Risk Assessment (OPPERA) project, a study of temporomandibular disorders (TMD), motivates this work. Numerous measures of interest are collected at enrollment, such as the number of comorbid pain conditions from which a participant suffers. Examining the potential genetic basis of these measures is of secondary interest. Assessing these associations is statistically challenging, as participants do not form a random sample from the population of interest. Standard methods may be biased and lack coverage and power. We propose a general method for the analysis of arbitrary phenotypes utilizing inverse probability weighting and bootstrapping for standard error estimation. The method may be applied to the complicated association tests used in next-generation sequencing studies, such as analyses of haplotypes with ambiguous phase. Simulation studies show that our method performs as well as competing methods when they are applicable and yield promising results for outcome types, such as time-to-event, to which other methods may not apply. The method is applied to the OPPERA baseline case–control genetic study.
Multistream 3-D Convolution Neural Network With Parameter Sharing for Human State Estimation

The large amount of data is one challenge in electroencephalogram (EEG) analysis, in which the channel Two Dimensional (2-D), time One Dimensional (1-D), and spectral (1-D) are generally considered. Convolutional neural networks (CNNs) have drawn much attention for automatic feature learning in various fields. Meanwhile, many studies have demonstrated integration from multiple sources and decisions could boost performance. However, CNN for EEG analysis usually involves millions of parameters, which easily leads to overfitting. A new model of a multistream Three Dimensional (3-D) CNN with parameter sharing is proposed for EEG. Two EEG data sets: 1) the lane-keeping task (LKT) data set and 2) sleep data set are applied. For the LKT data set, the proposed multistream 3-D CNN with parameter sharing model achieves 0.5486 root-mean-square error (RMSE), showing improvement by at least 2.77% compared to the other approaches. In the sleep data set, the error rate of the proposed model was 24.65%, showing at least 10.28% improvement in performance compared to the other methods. The lower RMSE and error rate show that the multistream 3-D CNN with parameter sharing model efficiently extracts significant features from EEG data. Moreover, the sharing mechanism even reduces the risk of overfitting and the number of parameters by comprehending common representations among multiple streams.
Macronutrient Intake and Insulin Resistance in 5665 Randomly Selected, Non-Diabetic U.S. Adults

The main goal of this investigation was to evaluate the relationships between several macronutrients and insulin resistance in 5665 non-diabetic U.S. adults. A secondary objective was to determine the extent to which the associations were influenced by multiple potential confounding variables. A cross-sectional design and 8 years of data from the 2011–2018 National Health and Nutrition Examination Survey (NHANES) were used to answer the research questions. Ten macronutrients were evaluated: total carbohydrate, starch, simple carbohydrate, dietary fiber, total protein, total fat, saturated, polyunsaturated, monounsaturated, and total unsaturated fat. The homeostatic model assessment (HOMA), based on fasting glucose and fasting insulin levels, was used to index insulin resistance. Age, sex, race, year of assessment, physical activity, cigarette smoking, alcohol use, and waist circumference were used as covariates. The relationships between total carbohydrate intake (F = 6.7, p = 0.0121), simple carbohydrate (F = 4.7, p = 0.0344) and HOMA-IR were linear and direct. The associations between fiber intake (F = 9.1, p = 0.0037), total protein (F = 4.4, p = 0.0393), total fat (F = 5.5, p = 0.0225), monounsaturated fat (F = 5.5, p = 0.0224), and total unsaturated fat (F = 6.5, p = 0.0132) were linear and inversely related to HOMA-IR, with 62 degrees of freedom. Starch, polyunsaturated fat, and saturated fat intakes were not related to HOMA-IR. In conclusion, in this nationally representative sample, several macronutrients were significant predictors of insulin resistance in U.S. adults.
Studies on the Rice RVA Profile Characteristics and Its Correlation with the Quality

Rice quality is a key factor and widely concerned by rice breeders and consumers, but it is difficult to evaluate it only through physical and chemical indices. In this study, 114 and 105 varieties including indica, japonica and waxy varieties were employed in two replicates to study the relationship between RVA profile characteristics and rice quality, including the appearance quality, cooking physico-chemical factor and cooking quality. The main results were as follows: (1) RVA profiles characteristics had close relationships with translucency and chalkiness. Rice varieties with higher chalkiness had significant AAC, HPV, CPV, SBV and CSV. (2) RVA profiles characteristics had significant correlation with AAC and GC, but no significant with GT. (3) With the exception to PKV, all the other RVA profiles characteristics correlated significantly with eating quality. These results showed that RVA profiles characteristics could be used as indices to assist in varietal selection for good cooking rice quality.
Association between Functional Movement Screen Scores and Athletic Performance in Adolescents: A Systematic Review

This study systematically reviews the literature examining the relationship between Fundamental Movement Screen (FMS©) scores and athletic performance in youth. We searched English-language papers on PubMed/MEDLINE, SportsDiscus, CINAHL, and EBSCO for the following inclusion criteria: Participants aged between 11 and 17 years, studies had to include the Functional Movement Screen© (FMS©) and at least one of the following performance outcomes, highlighted by athletic development models (i.e., long-term athletic development (LTAD), youth physical development (YPD)): agility, speed, power, strength, endurance, and balance (YPD), fitness (LTAD), or sport-specific skill (LTAD and YPD). A total of 3146 titles were identified, with 13 relevant studies satisfying the inclusion criteria after full-text screening. The results of this systematic review suggest that children and youth who score highly on the FMS© also tend to have better scores for agility, running speed, strength, and cardiovascular endurance. The strength of associations was weak to moderate in nature. Only one study was considered or controlled for biological maturation in their analysis. These results provide evidence that, while there is a relationship between FMS© scores and tests of athletic performance in youth, they are not the same thing and should be considered conceptually different constructs.
Street Clinics and the Healthcare of Vulnerable Homeless Communities in Brazil: A Qualitative Study

(1) Background: homeless people are characterized by serious social vulnerability and difficulty in accessing health services worldwide. In Brazil, this population is supported by the Street Clinic teams who are challenged to establish intersectoral networks to expand access and promote unique and humanized care from the perspective of harm reduction. The study aimed to analyze the practices of professionals working at the Street Clinic in a city in the interior of São Paulo, Brazil, to tackle the vulnerabilities of the homeless population and expand access to the health care network. (2) Methods: a qualitative study was conducted with a social approach in which we interviewed eight workers from the Street Clinic. Data were analyzed using the thematic content analysis tool. (3) Results: three nuclei of meaning were built: stigma and prejudice as the main barriers to accessing services, harm reduction as a humanized care practice and valuing autonomy, and Street Clinic as a gateway to the health system and main interlocutor with other services. (4) Conclusions: the articulation with network services is marked by contradictory relationships, of conflict and trust, signaling the need for greater investment in educational qualifications and working conditions for professionals at all levels of care to expand access to health care.
A Narrative Review of the Launch and the Deployment of Telemedicine in Italy during the COVID-19 Pandemic

Telemedicine is making an important contribution to the fight against the COVID-19 pandemic and to supporting the health domain. Its use has registered initial problems with often-patchy practise. The objective of this study was to analyze the launch and deployment of telemedicine in Italy through a narrative review. The narrative review faced two points of view: (a) the first point of view revised the institutional initiatives of the Italian government developed to promote the use of telemedicine; (b) the second point of view reviewed the evolution of scientific literature in the sector, with reference to the Italian situation. In the second point of view, we applied both a standard narrative checklist and an eligibility approach. The first point of view reported an analysis of national documents aimed at promoting, through indications and recommendations, the use of telemedicine. The second point of view analyzed 39 qualified references. The analysis highlighted: (a) that initially, there was a disorientation, followed by reflections that emerged immediately after; (b) a telemedicine application not only in the traditional sectors (e.g., diabetology, cardiology, oncology, neurology) but also in new and fields never explored before; and (c) a high level of acceptance and a desire to continue in the after-pandemic future (which emerged in some studies through dedicated questionnaires). The study offers stimuli for both stakeholders and scholars to improve the use of telemedicine during the pandemic and in the future.
Spatial Release from Masking for Tones and Noises in a Soundfield under Conditions Where Targets and Maskers Are Stationary or Moving

Stationary visual targets often become far more salient when they move against an otherwise static background–the so-called “pop out” effect. In two experiments conducted over loudspeakers, we tested for a similar pop-out effect in the auditory domain. Tone-in-noise and noise-in-noise detection thresholds were measured using a 2-up, 1-down adaptive procedure under conditions where target and masker(s) were presented from the same or different locations and when the target was stationary or moved via amplitude-panning. In the first experiment, target tones of 0.5 kHz and 4 kHz were tested, maskers (2–4, depending on the condition) were independent Gaussian noises, and all stimuli were 500-ms duration. In the second experiment, a single pink noise masker (0.3–12 kHz) was presented with a single target at one of four bandwidths (0.3–0.6 kHz, 3–6 kHz, 6–12 kHz, 0.3–12 kHz) under conditions where target and masker were presented from the same or different locations and where the target moved or not. The results of both experiments failed to show a decrease in detection thresholds resulting from movement of the target.
Defining Wound Healing Progression in Cetacean Skin: Characteristics of Full-Thickness Wound Healing in Fraser’s Dolphins (Lagenodelphis hosei)

Simple Summary Cutaneous wound healing is a complex and tightly regulated biological process to restore physiological and anatomic function. Current knowledge of cutaneous wound healing is mostly based on studies in laboratory animals and humans. The histological and immunological features of skin, for example, cutaneous thickness, cellular components, and immune response, are not identical among animal species, and these differences may lead to substantial effects in cutaneous wound healing. In field observation, large cutaneous wounds in cetaceans could heal without medical treatments. However, little is known about the underlying mechanisms, and there is no histological study on full-thickness wound healing in cetaceans. The current study characterizes the macroscopic and histological features of large full-thickness wound healing in Fraser’s dolphins (Lagenodelphis hosei). The differences of wound healing between cetaceans and terrestrial mammals were shown from the histological aspect, including rete and dermal ridge appearance, repigmentation, and adipose tissue regeneration. Better understanding of the mechanism of full-thickness wound healing in cetaceans will shed light on veterinary and human regenerative medicine, leading to novel therapies. Abstract Cetaceans are tight-skinned mammals that exhibit an extraordinary capacity to heal deep soft tissue injuries. However, essential information of large full-thickness wound healing in cetaceans is still lacking. Here, the stages of full-thickness wound healing were characterized in Fraser’s dolphins (Lagenodelphis hosei). The skin samples were collected from normal skin and full-thickness cookiecutter shark (Isistius brasiliensis)-bite wounds of stranded carcasses. We defined five stages of wound healing according to macroscopic and histopathological examinations. Wounds in Stage 1 and 2 were characterized by intercellular and intracellular edema in the epidermal cells near the wound edge, mixed inflammatory cell infiltration, and degradation of collagen fibers. In Stage 3 wounds, melanocytes, melanin granules, rete and dermal ridges were noticed in the neo-epidermis, and the adipose tissue in adjacent blubber was replaced by cells and fibers. Wounds in Stage 4 and 5 were characterized by gradual restoration of the normal skin architecture including rete and dermal ridges, collagen bundles, and adipose tissue. These phenomena were quite different from previous studies in terrestrial tight-skinned mammals, and therefore, further in-depth research into the mechanisms of dolphin wound healing would be needed to gain new insights into veterinary and human regenerative medicine.
Neonatal Jaundice and Autism: Precautionary Principle Invocation Overdue

Meta-analyses consistently find a substantial possible association between neonatal jaundice (hyperbilirubinemia) and later autism risk. The obvious question this poses is "what is the source of this risk? " This review explores the complementary roles of jaundice severity and time, racial and geographic disparities, and early infant feeding regime change, and discusses potential implications of these findings. A range of factors appears to increase the risk of autism development following neonatal jaundice, all of which are associated with the "exclusive breastfeeding" paradigm. Severity presents an intuitive risk factor in the context of bilirubin neurotoxicity; jaundice from the modal root cause of insufficient milk intake progresses as that condition persists. Racial and geographic disparities present another intuitive set of risk factors, including a heightened risk of missed diagnosis for darker-skinned neonates and delayed care access in poorer settings. In addition to these intuitive factors, near- or full-term as opposed to preterm status and phototherapy treatment may also heighten risk. These counter-intuitive findings provide additional support for deprivation/starvation as a crucial antecedent or independent variable, and time as a mediator to progression in and subsequent risk from jaundice; heightened medical monitoring and supplementation seem to protect preterms, and phototherapy risks iatrogenesis, having replaced without sufficient safety evidence the prior standard treatment of switching jaundiced, breastfed babies to formula. Critically, jaundice associated with insufficient milk intake due to breastfeeding insufficiencies is fully preventable and trivially treatable with appropriate supplemental milk. Feeding neonates adequately may play an important role in preventing autism and other neurodevelopmental disorders including attention deficit hyperactivity disorder, cerebral palsy, epilepsy, hearing impairment, learning disorders, and mood disorders. Precautionary principle invocation is overdue.
Eucharistic celebration and spiritual capital: the experience of students in a Spanish Catholic school – A research report inspired by the work of Dr Ann Casson, UK (2013)

There is clear evidence of increasing secularisation across Europe; nevertheless, the authority of the Christian tradition persists. This study focuses on the case of Spain, a well-founded Catholic origin country, which, in just a few decades has become from being one of the most religious to one of the least among Catholics. This investigation presents the experience of the spiritual capital generated by the Eucharistic celebration, viewed from the perspective of young students, and expressed in their own words. The Eucharistic celebration is still considered to be a spiritual ‘nutrition’ for most Catholic people; however, is it for the young generation? Our results indicate that the experience of ‘being Catholic’ is understood outside of the traditional forms of Catholic practices. The essential Catholic practice, the Mass, is no longer recognised as a ‘fingerprint’ of Catholicity. Nevertheless, when a sample of students were asked about the Mass, they understood the Eucharistic celebration in terms of the spiritual capital, as a source of values of solidarity, well-being, recognition of social injustices, experiences of joy, strength, feelings of gratitude, etc. However, only 8% of the students declare to participate regularly in the Mass; and, more than 50% of young people do not benefit from this resource of spiritual capital deposited in the Eucharist. Therefore, its connection with regular practice has become tenuous. One of the possible reasons for such a situation is the lack of understanding of the Mass and its spiritual, social and symbolic importance, and also by the lack of youth participation in active roles in the celebration of the Mass.
Determination of the Relationships between the Chemical Structure and Antimicrobial Activity of a GAPDH-Related Fish Antimicrobial Peptide and Analogs Thereof

The structure–activity relationships and mode of action of synthesized glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-related antimicrobial peptides were investigated. Including the native skipjack tuna GAPDH-related peptide (SJGAP) of 32 amino acid residues (model for the study), 8 different peptide analogs were designed and synthesized to study the impact of net charge, hydrophobicity, amphipathicity, and secondary structure on both antibacterial and antifungal activities. A net positive charge increase, by the substitution of anionic residues or C-terminal amidation, improved the antimicrobial activity of the SJGAP analogs (minimal inhibitory concentrations of 16–64 μg/mL), whereas the alpha helix content, as determined by circular dichroism, did not have a very definite impact. The hydrophobicity of the peptides was also found to be important, especially for the improvement of antifungal activity. Membrane permeabilization assays showed that the active peptides induced significant cytoplasmic membrane permeabilization in the bacteria and yeast tested, but that this permeabilization did not cause leakage of 260 nm-absorbing intracellular material. This points to a mixed mode of action involving both membrane pore formation and targeting of intracellular components. This study is the first to highlight the links between the physicochemical properties, secondary structure, antimicrobial activity, and mechanism of action of antimicrobial peptides from scombrids or homologous to GAPDH.