Miracle-dz/UniFAR-FTUs huggingface dataset:
Dose-response modelling of total haemoglobin mass to hypoxic dose in elite speed skaters

The aim of the present study is the modelling of the total haemoglobin mass responses in altitude environment with the dose-response model in elite endurance athletes and comparison different existing approaches in the quantification of hypoxic dose. Data from seven healthy elite endurance athletes specialised in middle distance speed skating participated in the study: six males (24±1.8 years, 182 ±0.3 cm, 84 ±1.5 kg, BMI 23.2±0.6 kg/m2, 59.3±1.5 ml/kg/min) and one female (21 years, 164 cm, 56 kg, BMI 17.1 kg/m2, 59.9 ml/kg/min). Data were collected during a 3-month training period which included two training camps (14 +14 days) at sea level and two training camps (21+21 days) at altitude of 1224 m and 1850 m above sea level. Total haemoglobin mass (tHb-mass) were measured before the start of the season (baseline) and before and after each training camp (seven measurements) using an optimized CO-rebreathing method, training loads and oxygen saturation at altitude were measured and hypoxic dose were calculated. Mean total haemoglobin mass for the male group at the base line were 1067±83 g, before the training camp 1 were 1095±82 g, after TC1 1113±105 g, before the training camp 2 (TC2) 1107±88 g, after TC2 1138±104 g. For the female athlete at the base line were 570 g, after TC1 564 g, after TC2 582 g. The increase of tHb-mass after TC2 were 3,25% and were significant (p<0,005). Mean hypoxic dose for the male group TC1 were %·h (98%) 1078±157, %·h (95%) 79±57, and km.h 473±1 and at TC2 were %·h(98%) 1586±585, %·h (95%) 422±182, and km.h 893±18 and were different from TC1 (p<0,05) for %·h (95%) and km.h methods. For the female athlete hypoxic dose at TC1 were %·h (98%) 970, %·h (95%) 32, and km.h 470 and at TC2 were %·h(98%) 1587, %·h (95%) 289, and km.h 900. The relationship between hypoxic dose and haematological response was analysed with a non-linear model. The magnitude of the increase of the total haemoglobin mass were investigated using simulation procedures based upon individual responses to the hypoxic dose. We introduced a measurement error to the list square method as a way of avoiding overfitting problem. Dose-response mathematical model between hypoxic dose and total haemoglobin mass was developed. Modelled total haemoglobin mass was within measurement error range. This model is suitable for the computer simulations. The individual response to hypoxic dose due to model data was different. Maximal values in total haemoglobin mass that can be achieved by male athletes according to the model was 1321.9 ± 32 g. The model predicted that (τ) erythrocyte life span is 73.8 ± 9.0 days. Moreover, highest value of individual tHb-mass increase after returning to the sea level according to the model was16.3 ±0.7 days. The model developed in the current study describes the time course of total haemoglobin mass during altitude exposure and post-altitude decline in elite speed skaters.
Population genomics of North American northern pike: variation and sex-specific signals from a chromosome-level, long read genome assembly

We present a chromosome-level, long-read genome assembly as a reference for northern pike (Esox lucius) where 97.5% of the genome is chromosome-anchored and N50 falls at 37.5 Mb. Whole-genome resequencing was genotyped using this assembly for 47 northern pike representing six North American populations from Alaska to New Jersey. We discovered that a disproportionate frequency of genetic polymorphism exists among populations east and west of the North American Continental Divide (NACD), indicating reproductive isolation across this barrier. Genome-wide analysis of heterozygous SNP density revealed a remarkable lack of genetic variation with 1 polymorphic site every 6.3kb in the Yukon River drainage and one every 16.5kb east of the NACD. Observed heterozygosity (Ho), nucleotide diversity (π), and Tajima’s D are depressed in populations east of the NACD (east vs. west: Ho: 0.092 vs 0.31; π: 0.092 vs 0.28; Tajima’s D: -1.61 vs -0.47). We confirm the presence of the master sex determining (MSD) gene, amhby, in the Yukon River drainage and in an invasive population in British Columbia and confirm its absence in populations east of the NACD. We also describe an Alaskan population where amhby is present but not associated with male gender determination. Our results support that northern pike originally colonized North America through Beringia, that Alaska provided an unglaciated refugium for northern pike during the last ice age, and southeast of the NACD was colonized by a small founding population(s) that lost amhby.
Alteration of Gut Microbiota in Autism Spectrum Disorder: An Overview

The microbiota-gut-brain axis, which refers to the bidirectional communication pathway between gut bacteria and the central nervous system, has a profound effect on important brain processes, from the synthesis of neurotransmitters to the modulation of complex behaviors such as sociability and anxiety. Previous studies have revealed that the gut microbiota is potentially related to not only gastrointestinal disturbances, but also social impairment and repetitive behavior—core symptoms of autism spectrum disorder (ASD). Although studies have been conducted to characterize the microbial composition in patients with ASD, the results are heterogeneous. Nevertheless, it is clear that there is a difference in the composition of the gut microbiota between ASD and typically developed individuals, and animal studies have repeatedly suggested that the gut microbiota plays an important role in ASD pathophysiology. This possibility is supported by abnormalities in metabolites produced by the gut microbiota and the association between altered immune responses and the gut microbiota observed in ASD patients. Based on these findings, various attempts have been made to use the microbiota in ASD treatment. The results reported to date suggest that microbiota-based therapies may be effective for ASD, but largescale, well-designed studies are needed to confirm this.
Origin of trunk damage in West African savanna trees: the interaction of fire and termites

Abstract: Two main types of hollow tree are frequently found in savannas: trees with external splits (externally damaged trees), and trees with no or little visible external damage, but with their entire core removed (internally damaged or ‘piped’ trees). As this may affect trunk mechanical resistance and tree survivorship, we studied the incidence of these two types of cavity in relation to two possible causal agents, fire and termites, in a West African savanna. Overall, the proportion of damaged adult trees (height >2 m) reached 36%, and up to 84% for Crossopteryx febrifuga. In this species, almost all (93%) damaged individuals showed signs of digging by fungus-grower and wood-feeder termites. External damage was more frequent in the more fire-prone shrubby savanna, suggesting that while termites are responsible for the piping, fire is responsible for the later opening of the trunk. Trees growing in the more fire-prone savanna tended to reach significantly smaller sizes, both in height and basal diameter, than in the less intensely burnt woody savanna. There was also evidence that piped trees were taller than externally damaged trees. This strongly suggests that fire causes an increased mortality of adult trees through lateral opening of the trunks causing later breakage.
Confirmation of morphophysiological dormancy in sandalwood (Santalum album, Santalaceae) seeds

Indian sandalwood ( Santalum album L.) is a hemi-parasitic angiosperm tree grown in India, Indonesia and Sri Lanka primarily for its economically valuable santalum oil. Although the seeds of this species have been reported to have morphophysiological dormancy (MPD), the occurrence of MPD in them or in Santalaceae has never been confirmed. Thus, the present study was conducted with the objective of identifying the class of dormancy present in S. album seeds. Ripened fruits of S. album collected from trees in Peradeniya, Sri Lanka were used to study the (1) imbibition and germination of non-scarified and scarified seeds, (2) effects of ambient laboratory conditions, gibberellic acid (GA3) and dry storage on germination and (3) embryo:seed length (E:S) ratio for fresh seeds and for seeds soon after radicle emergence. Nonscarified and scarified seeds readily imbibed water. Increased germination with GA3 indicated a physiological component to dormancy, and increased E:S ratio prior to radicle emergence revealed an additional morphological component. Thus, we confirmed the occurrence of MPD in S. album , and suggest that the level is non-deep simple. J.Natn. Sci.Foundation Sri Lanka 2015 43 (3): 209-215
Pumpkin powder (Cucurbita maxima)-supplemented string hoppers as a functional food

Background: Pumpkin has frequently been used as a functional food due to its nutritional and health benefits. In this study, a new application of dried pumpkin powder in string hoppers production is shown. Aim: The main aim of this work is to evaluate the addition of the dried pumpkin powder into rice flour on the physical, functional, nutritional, and sensory properties of string hoppers. Materials and Methodology: String hoppers were prepared using white rice flour, which was substituted at 0%, 10%, 15%, and 20% with pumpkin pulp powder. Sensory evaluation was conducted to select best accepted combination. Nutrition analysis was carried out for the best accepted product. Results: On sensory evaluation, 20% pumpkin pulp powder-incorporated string hoppers received the highest scores for appearance (8.06), color (7.93), aroma (8.02), taste (7.80), texture (7.93), and overall acceptability (8.13). This was selected as the best accepted pumpkin-incorporated string hoppers. The nutrient composition of 20% pumpkin pulp powder-incorporated string hoppers and control was moisture (47.79, 51.38%), ash (1.22, 0.059%), protein (6.12, 3.68%), and crude fiber (0.72, 0.29%), and significant increase of nutrients was observed in pumpkin pulp powder-incorporated string hoppers compared to control. Beta carotene content of the accepted string hoppers increased significantly (2.54 mg/100 g). Significant increase in potassium, calcium, magnesium, and phosphorus content was also observed (227.2 mg, 16.44 mg, 19.83 mg, and 18.83 mg/100 g, respectively). Nearly 20% addition of pumpkin pulp powder increased the antioxidant activity significantly (0.056 mmol/ascorbic acid equivalents/100 g). Conclusion: Pumpkin pulp powder can be successfully supplemented into the traditional string hoppers and used as a functional food with its improved nutritional composition.
Hospital preparedness and management of pediatric population during COVID-19 outbreak

With the recent pandemic of Coronavirus disease-2019 (COVID-19), there has been a higher number of reported cases in children more than to the prior Corona Virus-related diseases, namely, severe acute respiratory syndrome and the Middle East respiratory syndrome. The rate of COVID-19 in children is lower than adults; however, due to high transmission rate, the number of reported cases in children has been increasing. With the rising numbers among children, it is imperative to develop preparedness plans for the pediatric population at the hospital level, departmental level, and patient care areas. This paper summarizes important considerations for pediatric hospital preparedness at the hospital level that includes workforce, equipment, supply; capacity planning, and infection prevention strategies, it also span over the management of COVID-19 pediatric patients in high-risk areas such as critical care areas, Emergency Department and operative rooms.
Effects of Continuous Cultivation on Ferrosols in Subtropical Southeast Queensland. I. Site characterization, Crop Yields and Soil Chemical Status

The productivity of Ferrosols used for rainfed agricultural production in the south and central Burnett regions of south-east Queensland was examined in relation to the duration under continuous cultivation. A range of crops grown in on-farm situations during 1986-90 were examined using paired sites to assess the extent of yield decline with time under cropping. The changes in soil chemical characteristics that have occurred during the cropping period were also assessed. All locations showed evidence of a significant reduction in crop growth (50-100%) where continuously cropped sites were compared with sites which had either never been cropped or which had been under grazed grass pasture for >20 years. In the absence of severe late season water deficits, this reduced growth rate was always reflected in lower (21-72%) crop yields at maturity. However, crop dry matter (DM) could interact with crop water use under conditions of late-season water deficit to negate, or even reverse, early growth advantages on previously untilled soil. At least part of the observed yield reduction on continuously cropped soil was due to nutrient deficiencies resulting from depletion of both surface and subsurface reserves during cropping. Long-term cropping has resulted in depletion of soil K and Zn (especially in the subsoil), organic carbon and total N status, and caused significant acidification of both surface and subsoil layers despite the use of lime. The decline in subsoil K status and falling subsoil pH have severe implications for crop performance in dry seasons, when crops rely on subsoil reserves to sustain crop growth. The decline in soil N status has occurred despite a high frequency (>50%) of grain legumes in the crop rotations practised on all farms monitored, and illustrates the small N return from these crops under rainfed conditions. The reduction in soil organic carbon due to cropping was extreme, with continuously cropped areas having organic carbon levels of only 0.9 to 1.5% in the 0-10 cm layer-values which were only 25-40% of levels in untilled soil. Grazed grass leys were only partly successful in restoration of soil organic carbon status.
Tongue and its ties: Posterior tongue width in gender estimation – A forensic gratuity

Background and Objectives: Human identification is one of the challenging areas that man has been confronted with. The forensic odontologist deals with human identification based on unique features of the oral cavity. The human tongue is sheathed within the oral cavity, where it lies protected against the external atmosphere, just as the palatine folds. It is can be easily showed for inspection but at the same time be protected from the external environment. Aim and Objective: The objective of this study is to examine the uniqueness of tongue morphology and find out parameters to estimate gender of an individual using the same. Methodology: A total of 30 subjects (15 girls and 15 boys) were taken for this study whose tongue were examined, photographed and alginate impression were taken to create a database of tongue morphology. Result: The mean posterior width of the tongue was higher among the males in both cast as well as photographs.
Microneedle-Based Delivery: An Overview of Current Applications and Trends

Microneedle arrays (MNA) are considered as one of the most promising resources to achieve systemic effects by transdermal delivery of drugs. They are designed as a minimally invasive, painless system which can bypass the stratum corneum, overcoming the potential drawbacks of subcutaneous injections and other transdermal delivery systems such as chemical enhancers, nano and microparticles, or physical treatments. As a trendy field in pharmaceutical and biomedical research, its applications are constantly evolving, even though they are based on very well-established techniques. The number of molecules administered by MNA are also increasing, with insulin and vaccines administration being the most investigated. Furthermore, MNA are being used to deliver cells and applied in other organs and tissues like the eyes and buccal mucosae. This review intends to offer a general overview of the current state of MNA research, focusing on the strategies, applications, and types of molecules delivered recently by these systems. In addition, some information about the materials and manufacturing processes is presented and safety data is discussed.
Maternal and neonatal response to COVID-19

The risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to maternal and newborn health has yet to be determined. Several reports suggest pregnancy does not typically increase the severity of maternal disease; however, cases of preeclampsia and preterm birth have been infrequently reported. Reports of placental infection and vertical transmission are rare. Interestingly, despite lack of SARS-CoV-2 placenta infection, there are several reports of significant abnormalities in placenta morphology. Continued research on pregnant women infected with SARS-CoV-2 and their offspring is vitally important.
Genetic transformation in commercial Tasmanian cultivars of opium poppy, Papaver somniferum, and movement of transgenicpollen in the field.

We report a new transformation protocol for the pharmaceutically important opium poppy, Papaver somniferum L. ; the protocol allows transformation for the first time of high yielding commercial cultivars. The method involves Agrobacterium tumefaciens infection of hypocotyl explants, followed by the production of antibiotic- or herbicide-resistant embryogenic callus and the subsequent induction of somatic embryos and plants. Key elements of the improvement are the use of buffering agents to stabilise medium pH and bottom-cooling of the cultures. Transformation was verified by PCR and Southern blot hybridisation. Transcription of transgenes was confirmed by RT-PCR and product sequencing. Expression of transgenes was detected by histochemical GUS staining, phosphinothricin acetyltransferase (PAT) enzyme assays for bar and pat genes, and western analysis of transgenic sunflower seed albumin protein. Expression of various transgenes was detected in stem, leaf, seed, capsule and latex. The pat gene was demonstrated to be stably inherited to the T2 generation and to confer phosphinothricin (PPT) herbicide resistance. Most T0 plants showed normal morphology, were self-fertile and the transgenes displayed the expected Mendelian segregation. The percentage of explants producing somatic embryos that developed into plantlets able to be transplanted to soil, ranged from 6-11% in two Tasmanian cultivars.A field trial using pat transgenic plants was designed to estimate the frequency of hybridisation at various distances into buffer rows of non-transgenic poppies and to related weed species, P. somniferum spp. setigerum and P. dubium. The frequency of hybridisation to completely compatible poppy fell sharply with distance, being 2.6% at 20 cm, 2.13% at 0.5 m and falling to 0% at 2.5 and 5 m. No hybridisation could be detected to two weed species under open pollination conditions, including the compatible P. somniferum spp. setigerum, when grown as close as 20 cm, despite flowering at the same time as the transgenic plants in the presence of foraging bees.
Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China

Combining phenotypic and genetic characteristics in a genetic variation study is of paramount importance to effectively orient the selection of producers’ elite trees in a seed orchard. In total, 28 phenotypic characteristics and 16 microsatellite loci were used to analyze the clonal genetic variation, to characterize the genetic diversity, and to refine the genetic classifications of 110 Pinus koraiensis clones grown in the Naozhi orchard in northeastern China. All clones were significantly different in most traits. Most of the phenotypic characteristics showed great genetic variation among clones, while the genotypic differentiation was weak between the selection sites of clones. The SSR markers showed a relatively high level of genetic diversity (Na = 4.67 ± 0.43, Ne = 2.916 ± 0.18, I = 1.15 ± 0.07, Ho = 0.69 ± 0.04, He = 0.62 ± 0.02, and mean polymorphic information content (PIC) of 0.574), with higher heterozygosity as an indication of a lower probability of inbreeding events in the orchard. Despite weak correlation coefficients between dissimilarity matrices (r(A/B), range equal to 0.022, p-value < 0.001), the genetic and phenotypic classifications congruently subdivided all the clones into three major groups. The patterns of phenotypic trait variations and genetic diversity are valuable to effectively select materials in breeding programs of P. koraiensis.
Analysis of determinants of mammalian species richness in South America using spatial autoregressive models

Classically, hypotheses concerning the distribution of species have been explored by evaluating the relationship between species richness and environmental variables using ordinary least squares (OLS) regression. However, environmental and ecological data generally show spatial autocorrelation, thus violating the assumption of independently distributed errors. When spatial autocorrelation exists, an alternative is to use autoregressive models that assume spatially autocorrelated errors. We examined the relationship between mammalian species richness in South America and environmental variables, thereby evaluating the relative importance of four competing hypotheses to explain mammalian species richness. Additionally, we compared the results of ordinary least squares (OLS) regression and spatial autoregressive models using Conditional and Simultaneous Autoregressive (CAR and SAR, respectively) models. Variables associated with productivity were the most important at determining mammalian species richness at the scale analyzed. Whereas OLS residuals between species richness and environmental variables were strongly autocorrelated, those from autoregressive models showed less spatial autocorrelation, particularly the SAR model, indicating its suitability for these data. Autoregressive models also fit the data better than the OLS model (increasing R2 by 5-14%), and the relative importance of the explanatory variables shifted under CAR and SAR models. These analyses underscore the importance of controlling for spatial autocorrelation in biogeographical studies.
Cervical epidural analgesia combined with general anesthesia for head and neck cancer surgery: A randomized study

Background and Aims: The role of cervical epidural analgesia in head and neck cancer surgery is not fully explored. The aim of this study was to evaluate cervical epidural analgesia in terms of opioid and anesthetic requirements and stress response in patients undergoing head and neck cancer surgery. Material and Methods: After institutional ethical committee approval and written informed consent, 30 patients undergoing elective head and neck cancer surgery were randomized into two groups: Group E (cervical epidural analgesia with general anesthesia), and group G (general anesthesia alone). In group E, an 18 gauge epidural catheter was placed at cervical (C) 6 – thoracic (T) 1 level. After test dose, a bolus of 10 ml of 0.2% ropivacaine was given followed by continuous infusion. Technique of general anesthesia and post-operative management was standardized in both the groups. Opioid and anesthetic drug requirement was observed. Blood glucose and serum cortisol levels were measured at baseline; post-incision and after surgery. Results: There was significant reduction in the requirement of morphine (P < 0.001), isoflurane (P = 0.004) and vecuronium (P = 0.001) in group E. Post-operative, blood glucose and serum cortisol levels were significantly reduced (P = 0.0153 and 0.0074, respectively). Early post-operative pain was reduced with the lesser requirement of post-operative morphine. Conclusions: The use of combined cervical epidural analgesia with general anesthesia reduces opioid, anesthetic drug requirement and stress response as compared to general anesthesia alone in patients undergoing head and neck cancer surgery.