Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular characterization of Potato virus Y (PVY) and Potato virus V (PVV) isolates naturally infecting cape gooseberry (Physalis peruviana) in Antioquia, Colombia

Due to the increase of the international demand for functional fruits, cape gooseberry ( Physalis peruviana ) has become one of the crops of highest expansion in Colombia and the Andean region of South America. nfortunately, the emergence of fungal and unidentified viral diseases has slowed down the cultivation of cape gooseberry in Colombia and, particularly, in the department of Antioquia. In this work, a next-generation sequencing virome analysis of cape gooseberry plants from eastern Antioquia was performed, using leaves exhibiting symptoms such as mosaics, leaf deformation and greening of veins. The complete genomes of Potato virus Y (PVY) and Potato virus V (PVV) were obtained in the assembled data. The presence of both viruses was confirmed in the samples obtained at two commercial cape gooseberry fields by real time RT-PCR (RT-qPCR) and partial Sanger sequencing of the coat protein (CP). Sequence analysis revealed significant sequence similarity between PVY and PVV isolates infecting P. peruviana to previously identified strains infecting potato ( Solanum tuberosum and S. phureja ) and tomato ( Solanum lycopersicum ) in the same geographical region. This study suggests that cape gooseberry could be an alternate host to viruses of other economically important solanaceous crops in the Andean region of South America.
Prostaglandin F2α-induced TGFβ-PMEPA1 pathway is a critical mediator of epithelial plasticity and ovarian carcinoma progression

Prostaglandin (PG) F2α has been scarcely studied in cancer. We have identified a new role for PGF2α in ovarian cancer, stimulating the production of TGFβ and the consequent induction of PMEPA1. We show that this induction increases cell plasticity and proliferation, enhancing tumor growth through PMEPA1. Thus, PMEPA1 overexpression in ovarian carcinoma cells, significantly increased cell proliferation rates, whereas PMEPA1 silencing decreased proliferation. In addition, PMEPA1 overexpression buffered TGFβ signaling, via reduction of SMAD-dependent signaling. PMEPA1 overexpressing cells acquired an epithelial morphology, associated to higher E-cadherin expression levels while β-catenin nuclear translocation was inhibited. Interestingly, in mouse xenografts, PMEPA1 overexpressing ovarian cells had a clear survival and proliferative advantage, resulting in higher metastatic capacity, while PMEPA1 silencing had the opposite effect. Furthermore, high PMEPA1 expression in a cohort of advanced ovarian cancer patients was observed, correlating with E-cadherin expression. Most importantly, high PMEPA1 mRNA levels were associated with lower patient survival. Significance This work identifies Prostaglandin F2α as an inducer, through NFAT, of TGFβ and together with this up-regulate PMEPA1, which is identified both as a drug target and as a prognostic biomarker in ovarian tumors, potentially useful in clinical practice.
The Impact of Formal Strength and Conditioning on the Fitness of Law Enforcement Recruits: A Retrospective Cohort Study.

Research involving law enforcement populations has suggested better fitness could enhance job task performance and reduce injuries. Academy training should lead to improvements in recruit fitness. The aim of this study was to investigate the impact of a strength and conditioning program on fitness among law enforcement recruits. Twenty-six recruits (23 males, three females) completed a 27-week academy, which incorporated 3-4 physical training sessions per week. Fitness assessment occurred during pre- (week 0), mid- (week 14), and post-testing (week 27) time points. The fitness assessments included: vertical jump, one-minute push-ups, one-minute sit-ups, posterior chain strength measured by a leg/back dynamometer, grip strength, and aerobic fitness measured by the 20-m multistage shuttle run (MSR). A repeated measures ANOVA with Bonferroni post hoc tests determined any significant changes in fitness between time points, with alpha set at p < . 05. Due to the small sample size of females, statistical analysis was only conducted on male recruits. Overall, significant main effects (p < .001) were observed in all fitness assessments except for grip strength. The results detailed general improvements in fitness. However, push-up and MSR scores decreased from mid- to post-test, while sit-ups did not change. Posterior chain strength and the vertical jump improved from mid-to post-test. The data indicated that the strength and conditioning program positively influenced the fitness of recruits. An increased focus on skill-specific work in the second-half of academy may have contributed to the plateaus in muscular endurance and aerobic fitness, and improvement of lower-body strength and power.
Influence of drought stress and application of ammonium sulfate on quality of rapeseed oil

a Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran b Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran c Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran d Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Study of the effect of silver nanoparticles encapsulated by doxorubicin drug in the treatment of hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is the third deadliest and fifth most common cancer worldwide. Many drugs that have the potential to treat cancers have had limited success due to their lack of efficient and safe delivery mechanisms that allow the drug molecules to cross cell membranes. Electrical pulses-mediated drug delivery, known as electroporation, is gaining attention as a possible approach to enhance uptake of chemotherapy. The present work studied the effect of silver nanoparticles encapsulated by doxorubicin drug in the treatment of hepatocellular carcinoma. The study was conducted on 40 albino mice weighting 20-25 g of 8-10 weeks of age, and were divided into two major groups. Group A:10 mice were used as a control , group B: 40 mice have induced HCC by DABE this group were subdivided into three subgroups; sub groupB1 10 mice which were not received any treatment, and were kept at room environment and, sub group B2: 10 mice were injected i.p with a dose of 50 mg/kg body weight of doxorubicin only every day for 21days, sub group B3 :10 mice were injected i.p. with a dose of 50 mg/kg body weight of doxorubicin encapsulated by the silver nanoparticles every day for 21days.Parts of liver tissue and blood samples were collected from all from mice of each group for histopathological examination; the nanocapsules were of the size range 200 ± 20 nm and loaded with the positively charged anticancer drug doxorubicin with an efficiency of 89%. The loading of the drug into the capsule occurs by virtue of the pH-responsive property of the capsule wall, which is determined by the pKa of the polyelectrolytes. As the pH is varied, about 64% of the drug is released in acidic pH while 77% is released in neutral pH. Molecular detection of Alpha-fetoprotein (AFP) and Glypican-3 (gly3) mRNAs by RT-PCR before and after treatment by silver nanoparticles encapsulated doxorubicin drug were studied in mice with HCC. Silver nanoparticles encapsulated doxorubicin drug showed effective results for treatment of HCC compared to results obtained with doxorubicin drug resulting in reduced tumor growth, and induction of apoptosis in the treated cells by enhancing of delivering chemotherapy and anticancer drugs with enhanced efficacy and fewer adverse effects.
EFFECT OF FEEDING ON SOME DIETARY FIBERS FOR REDUCING BLOOD CHOLESTEROL

The present study was designed to investigate the effect of some sources of dietary fibers (wheat bran, pectin and soybean hulls) at a level of 10% on the blood cholesterol level and serum transaminase enzymes, as well as, body weight, food intake, food efficiency ratio (FER) and some organs weight in hypercholesterolemic rats. The supplementation of basal diet with dietary fibers led to significant increase of final body weight, body weight gain (%), food intake and FER. Hypercholesterolemic rats had a higher level of total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and total cholesterol/high density lipoprotein-cholesterol (TC/HDL-C) and lower level of HDL-C comparing with normal rats. The addition of dietary fibers in all forms to basal diet improved the previous parameters and they became in the normal range. The feeding of hypercholesterolemic rats on diet containing dietary fibers decreased the liver content of total lipids, phospholipids, cholesterol and triglycerides (TG) to the level, where no significant difference was noticed with normal group. Also, the results revealed that, fecal output, fecal lipid and fecal cholesterol excretion were increased with supplementation of basal diet with dietary fibers. This supplementation improved serum transaminase enzymes especially alanine-aminotransferase enzyme (AST). So, it can be recommended that, using dietary fibers (wheat bran, pectin and soybean hulls) at the level of 10% had a pronounced effect in reducing cholesterol levels.
DeepSignal: detecting DNA methylation state from Nanopore sequencing reads using deep-learning

The Oxford Nanopore sequencing enables to directly detect methylation sites in DNA from reads without extra laboratory techniques. In this study, we develop DeepSignal, a deep learning method to detect DNA methylated sites from Nanopore sequencing reads. DeepSignal construct features from both raw electrical signals and signal sequences in Nanopore reads. Testing on Nanopore reads of pUC19, E. coli and human, we show that DeepSignal can achieve both higher read level and genome level accuracy on detecting 6mA and 5mC methylation comparing to previous HMM based methods. Moreover, DeepSignal achieves similar performance cross different methylation bases and different methylation motifs. Furthermore, DeepSignal can detect 5mC and 6mA methylation states of genome sites with above 90% genome level accuracy under just 5X coverage using controlled methylation data.
Exploration of the psychometric properties of the Montreal Battery of Evaluation of Amusia in a sample with temporal lobe epilepsy

Background The Montreal Battery of Evaluation of Amusia (MBEA) is a newly-developed instrument to assess music perception and memory, associated with temporal lobe functioning. Non-symptomatic temporal lobe epilepsy is a prevalent condition in Mexico, and it gives an opportunity to test the MBEA, considering the fact that epileptic seizures can cause neuropsychological impairment according to lobe localization and hemispherical lateralization of the epileptogenic focus. Objective To explore the psychometric and diagnostic properties of the MBEA. Method Two non-probabilistic samples of 31 control subjects and 22 cases with non-symptomatic temporal lobe epilepsy were assessed with the MBEA. Data from the original validation were used to compare with the control group. Results Analysis with the t test showed significantly lower performances in the case group relative to controls, and a general lower performance of controls compared to the norm. There was no significant difference in performance between cases with left epileptogenic focus and cases with right epileptogenic focus. ROC curve analysis showed questionable properties of sensitivity and specificity in the MBEA. Discussion and Conclusion Impairments in music perception were found in some cases, although theoretical inconsistencies with respect to relation between impaired functions were also detected. The performance of the control group relative to the norm indicated that the validation process should be continued, considering cultural differences. The MBEA seems to be a poor measure in terms of sensitivity and specificity for the detection of amusic impairments in subjects with non-symptomatic temporal lobe epilepsy, and its usefulness for determining hemispheric lateralization of epileptogenic focus remains uncertain.
AI in arcing‐HIF detection: a brief review

In the past few decades, the arcing-high-impedance fault (arcing-HIF) detection problems have become an important issue in the effectively grounded distribution network. Many solutions have been proposed to address this problem. The most attractive way is artificial intelligence (AI) method. The paper gives a comprehensive review of arcing-HIF detection in distribution network-based AI. First, characteristics and models of arcing-HIF are analysed, the arcing-HIF database construction method is also explained; this part is a foundation work for arcing-HIF detection. Next, arcing-HIF detection methods based AI are summarised in details including data acquisition, feature extraction and classifier selection. Then, a set of criteria are proposed to evaluate the reliability of arcing-HIF detection algorithm. Finally, the future trends and challenges to arcing-HIF detection are also fully accounted. This review can be a valuable guide for researchers who are interested in arcing-HIF detection-based AI.
Enquête ethnobotanique et criblage phytochimique de quelques plantes tinctoriales du Niger en vue d’une valorisation en énergie solaire

Les cellules solaires a pigments photosensibles semblent etre le dispositif photovoltaique d’avenir. Toutefois, la toxicite et le cout de synthese des pigments limitent l’utilisation accrue desdits prototypes. Les pigments naturels vegetaux peuvent constituer une alternative efficace, durable et bon marche. Dans cette etude, l’enquete ethnobotanique des plantes traditionnellement utilisees dans la teinture (textile, maroquinerie, poterie et esthetique) a ete realisee aupres des herboristes, des artisans teinturiers, des chercheurs nigeriens et des tradipraticiens de la region de Niamey. Par la suite, le criblage phytochimique, via des reactions colorees et/ou de precipitations de vingt plantes les plus citees, a ete realise. L’enquete ethnobotanique a permis de repertorier une quarantaine d’especes vegetales qui sont utilisees dans la production des colorants. Ces plantes sont reparties dans 23 familles dont les plus dominantes (62,5% des especes recensees) sont les Caesalpiniaceae (12,5%), les Combretaceae (10%), les Fabaceae (10%), les Anacardiaceae (10%), les Poaceae (5%), les Cochlospermaceae (5%), les Meliaceae (5%) et les Mimosaceae (5%). Des tannoides, flavonoides, anthocyanes, quinones, coumarines, alcaloides, sterols et polyterpenes sont detectes dans les extraits de ces plantes. L’abondance remarquable des diverses molecules colorantes fait de ces plantes des matieres premieres potentielles pour la production des colorants naturels valorisables en energie solaire, particulierement dans les cellules solaires a pigments photosensibles. Mots cles : Inventaire des plantes, pigments naturels, test phytochimique, energie solaire, Niger.
The Relationships Between Milk Constituents and Various Milk Properties in Anatolian Buffaloes

The objectives of this study were to investigate the relationships among milk composition, renneting time, urea concentration, acidity, density and pH of Anatolian Buffaloes’ milk. As a total of 115 individual milk samples from 53 Anatolian buffalo cows that calved in 2004 and 2005 on days of their lactations 30 ± 15, 60 ± 15, 90 ± 15, 120 ± 15, 150 ± 15, 180 ± 15, 210 ± 15, 240 ± 15 and 270 ± 15 in 8 units of Ilikpinar village were collected in morning milkings in June, September, December and March. Samples were analysed for total dry matter (TDM), fat, protein, ash, density, pH, acidity, renneting time and urea content. Data were classified according to the following environmental factors: lactation stages: 1 (30 ± 15, 60 ± 15, 90 ± 15 days): 2 (120 ± 15, 150 ± 15, 180 ± 15 days): 3 (210 ± 15, 240 ± 15, 270 ± 15 days); calving year: 1 (2004), 2 (2005); calving season: 1 (January-May), 2 (September and October); month of samples collection: 1 (June), 2 (September), 3 (December), 4 (March); lactation order: 1 and 2:1, 3 and 4:2, 5 and 6:3. Means and correlation coefficients for the characteristics investigated were calculated. There were negative significant correlations between daily milk yield with TDM, fat and protein percentages, and between pH and all of the milk constituents. Density reduces as TDM, fat and protein contents increase. Relationships between density and coagulation time with milk yield and pH were not significant. Relationships between milk urea concentration with none of milk constituents, milk yield, density, pH and titratable acidity were not significant statistically. It was concluded that genetic selection has to be directed towards increasing fat, protein and total not fat dry matter yields. Under selection programs in which milk yield is taken into consideration, fat and protein yields also increase, but fat and protein concentrations decrease.
Accelerated thermal aging behaviors of EPDM and NBR vulcanizates

The crosslink densities of rubber vulcanizates cured by a sulfur accelerator system are changed by thermal aging. Change of the crosslink density occurs by formation of new crosslinks and dissociation of existing crosslinks. Crosslink density change of a rubber vulcanizate with a high sulfur content after thermal aging was larger than that with a low sulfur content. Permanent deformation of a rubber vulcanizate results from the crosslink density change by thermal aging under the deformed state. Strain applied to a rubber vulcanizate affects the change of crosslink density. The crosslink density of the more strained vulcanizate was changed by thermal aging more than those of the less strained one. Ethylene-propylene diene rubber (EPDM) is a weatherresistant rubber and has slow cure characteristics of long induction time and slow cure rate. Acrylonitrile-butadiene rubber (NBR) is oil-resistant rubber and has fast cure characteristics of short induction time and fast cure rate. EPDM and NBR have different chemical properties each other. EPDM is nonpolar and has slow cure characteristics, whereas NBR is polar and has fast cure characteristics. In this work, we studied changes of crosslink densities of EPDM and NBR vulcanizates by thermal aging at high temperatures of 70-100 C for long time of 180 days. Levels of the crosslink density change and the activation energies of the vulcanizates were also compared. Thermal aging behaviors depending on the aging time were explained with the activation energy variation. Figures 1 and 2 show variations of the crosslink density changes of the EPDM and NBR vulcanizates with the aging time, respectively. The crosslink density change was obtained by dividing the difference in the crosslink densities after and before the thermal aging by the crosslink density of the initial sample. The crosslink density change increases as the aging time elapses. Level of the crosslink density change becomes larger by increasing the aging temperature. For the EPDM vulcanizate, the crosslink density change increases notably during the initial period and then the increment becomes smaller. The crosslink densities increase by 10.0, 17.6, and 22.6% after the thermal aging at 70, 85, and 100 C for 30 days, respectively, and increase by 17.3, 27.9, and 42.5% after the thermal aging for 180 days, respectively. The initial 30 day increments (0 to 30 days) are 10.0, 17.6, and 22.6% for 70, 85, and 100 C, respectively, while the final 30 day increments (150 to 180 days) are 0.2, 0.4, and 1.7%, respectively. For the NBR vulcanizate, the crosslink density change increases notably with increase of the aging time as shown in Figure 2. Especially, the crosslink density change at 100 Figure 1. Variation of the crosslink density change of the EPDM vulcanizate by the thermal aging as a function of the aging time. Squares, circles, and triangles indicate the aging temperatures of 70, 85, and 100 oC, respectively.
Taxonomic and Phylogenetic Investigations on Psoralea acaulis (Psoraleeae: Fabaceae) with the Description of a New Genus Kartalinia1

Abstract Psoralea acaulis Hoffm. is a very peculiar and taxonomically isolated species of the tribe Psoraleeae, occurring in the Caucasian territories. Currently, this tribe contains 10 distinct genera, well differentiated morphologically. Due to some morphological similarities, P. acaulis was attributed to Bituminaria Heist ex Fabr. , a genus with a Mediterranean–Macaronesian distribution. However, as it has many characters, some of which are autapomorphic, it was assigned its own subgenus: Christevenia Barneby ex C. H. Stirt. In light of detailed cladistic and phylogenetic analyses, this arrangement is taxonomically questionable and rather controversial. In order to clarify its status, extensive macromorphological, micromorphological, karyological, and palynological investigations of the currently known species of Bituminaria and all other genera of the tribe Psoraleeae were carried out. This allowed us to establish that P. acaulis should be placed in a separate genus in this tribe. According to the cladistic analysis of all known genera of Psoraleeae, this new monospecific genus, named Kartalinia Brullo, C. Brullo, Cambria, Acar, Salmeri & Giusso, is morphologically and karyologically very distinct and taxonomically isolated from Bituminaria, as well as from the other members of Psoraleeae. In this paper, the morphology and micromorphology of pod and seed testa, pollen grains, and seedlings; the karyology (2n=22); ecology; conservation status; and chorology of K. acaulis (Hoffm.) Brullo, C. Brullo, Cambria, Acar, Salmeri & Giusso are examined. An analytical key to all genera of Psoraleeae is also provided.
THE EFFECT OF UNSTRUCTURED GRID TOPOLOGY AND RESOLUTION ON SIMULATIONS OF DECAYING TURBULENCE

The effect of grid topology and resolution is studied for decaying, homogenous isotropic turbulence using unstructured grids. The grid topology is examined via an approach which includes grids of structured-like quality (ordered hexahedra) and also grids which consist entirely of random tetrahedra. The grid resolution is varied to include three levels for each topology. Decaying turbulence is chosen for its well-documented behavior and ease for examining statistical quantities. It is expected that not all turbulence models will behave the same on all grids. To limit such variability, Delayed Detached Eddy Simulations are considered exclusively. Metrics of merit include the energy spectra measured at two different times, the decay of the resolved turbulent kinetic energy, time history of the skewness and kurtosis of the velocity gradients, and the evolution of the transverse Taylor microscale. Data are compared to available experiments. The goal of this work is to provide guidance to users of industrial CFD codes, specifically BCFD, who are interested in unsteady simulation of turbulent flows. Results show a wide range of behavior with variation in model coefficient, grid resolution, and differencing scheme.