Miracle-dz/UniFAR-FTUs huggingface dataset:
Genome-Wide Association and Expression Analysis of the Lipoxygenase Gene Family in Passiflora edulis Revealing PeLOX4 Might Be Involved in Fruit Ripeness and Ester Formation

Aroma is an important factor in fruit quality. Passiflora edulis (passion fruit) is popular among consumers because of its rich flavor and nutritional value. Esters are the main components of the volatile aroma of passion fruit. Lipoxygenase (LOX), as the first key enzyme upstream of esters, may play an important role in the formation of passion fruit aroma. In this study, a total of 12 passion fruit LOX (PeLOX) members were screened out based on the Passiflora edulis genome database, which were distributed unevenly on 6 chromosomes, all containing the highly conserved lipoxygenase domain and some containing the PLAT domain. The gene structure, evolutionary analysis and cis-acting elements of the family members were predicted in this study. Transcriptome analysis showed that 12 PeLOX genes had different degrees of response to different abiotic stresses (drought stress, salt stress, cold stress, and high temperature). PeLOX1, PeLOX2, PeLOX7, PeLOX11, and PeLOX12 responded significantly to various abiotic stresses, while PeLOX8 and PeLOX9 had little change in expression in all stresses. Quantitative real-time PCR (qRT-PCR) in six tissues revealed that the 12 PeLOX genes exhibited tissue expression specificity, and the relative expression of most genes were particularly high in the roots, stems, and fruits. Focusing on passion fruit ripening and ester synthesis, the transcriptomic analysis showed that with the increase in fruit development and fruit maturity, the expression levels of PeLOX1, PeLOX9, PeLOX11, and PeLOX12 showed downregulated expression, while PeLOX2 and PeLOX4 showed upregulated expression. In particular, the upregulation trend of PeLOX4 was the most obvious, and the qRT-PCR results were consistent with the transcriptome result. Pearson correlation analysis showed that with the development and ripening of fruit, the expression level of PeLOX4, LOX enzyme activity and total ester content all showed an increasing trend, in particular during the period when the peel was red and shrank (from T2 to T3 stage), the esters’ contents increased by 37.4 times; the highest expression levels were all in the T3 period. The results indicated that PeLOX4 may be a candidate gene involved in fruit ripeness and the formation of volatile aroma compounds, with the increase in fruit ripening, the expression level of PeLOX4 increased and the LOX enzyme activity increased accordingly, thereby promoting the synthesis of volatile esters in fruit pulp. Our discovery lays the foundation for the functional study of LOX in passion fruit.
Prevalence and distribution of Alternaria leaf spot in soybean growing areas of Rajasthan

Soybean is one of the most important oil seed crops grown in India with high amount of protein and oil content in it. To understand the prevalence and distribution pattern of Alternaria leaf spot disease a study was carried out in six major soybean growing districts of Rajasthan during kharif 2018-19. The pathogen associated with leaf spot disease was isolated and identified as Alternaria alternata. The study revealed that the disease had more occurrences in those districts where soybean is mainly grown. The maximum percent disease index 22.02-29.08% and 37.52-42.35% was found in Udaipur region followed by Chittorgarh district having PDI 21.33-25.08 and 30.57-37.72% and minimum PDI (14.70-19.39 and 23.27-28.33%) was recorded in Kota region at flowering and pod formation stage during both the years. The favourable temperatures range of 21.8-29.4 °C and relative humidity in the range of 73.3-92.4% for occurrence, development and spread of this disease. The overall PDI was observed more in the year 2019 as compared to 2018. This disease was found to cause severe losses on a wider area under soybean cultivation in Rajasthan.
Comparing Well and Geophysical Data for Temperature Monitoring Within a Bayesian Experimental Design Framework

Temperature logs are an important tool in the geothermal industry. Temperature measurements from boreholes are used for exploration, system design, and monitoring. The number of observations, however, is not always sufficient to fully determine the temperature field or explore the entire parameter space of interest. Drilling in the best locations is still difficult and expensive. It is therefore critical to optimize the number and location of boreholes. Due to its higher spatial resolution and lower cost, four‐dimensional (4D) temperature field monitoring via time‐lapse Electrical Resistivity Tomography has been investigated as a potential alternative. We use Bayesian Evidential Learning (BEL), a Monte Carlo‐based training approach, to optimize the design of a 4D temperature field monitoring experiment. We demonstrate how BEL can take into account various data source combinations (temperature logs combined with geophysical data) in the Bayesian optimal experimental design (BOED). To determine the optimal data source combination, we use the Root Mean Squared Error of the predicted target in the low dimensional latent space where BEL is solving the prediction problem. The parameter estimates are accurate enough to use in BOED. Furthermore, the method is not limited to monitoring temperature fields and can be applied to other similar experimental design problems. The method is computationally efficient and requires little training data. For the considered optimal design problem, a training set of only 200 samples and a test set of 50 samples is sufficient.
Third BNT162b2 Vaccine Booster Dose against SARS-CoV-2-Induced Antibody Response among Healthcare Workers

This study assessed humoral response to the third BNT162b2 dose among healthcare workers (HCW). This prospective cohort study of HCW tested for anti-spike antibodies (LIAISON SARS-CoV-2 S1/S2 IgG assay) at 1, 3, 6, 9, and 12 months after receiving the second BNT162b2 vaccine dose (tests 1, 2, 3, 4, and 5, respectively). A third (booster) vaccination dose was introduced before test 4. Linear regression model was used to determine the humoral response following vaccine doses. For each serology test, changes in log-transformed antibody concentrations over time, adjusted for age, sex, underlying diseases, steroid treatment, and smoking were described using the general linear mix model. Serology tests were performed at 3, 6, 9, and 12 months after the second vaccine dose in 1113, 1058, 986, and 939 participants, respectively. The third dose was received by 964 participants before the 9-month tests, 797 of whom participated in the 9- and 12-month serology tests. A significant inverse correlation was noted between time from third dose and antibody concentrations (Spearman correlation −0.395; p < 0.001). Age (p < 0.0001; CI 95% −0.005–−0.004), heart disease (p < 0.0001; CI 95% −0.177–−0.052), immunodeficiency (p < 0.0001; CI 95% 0.251–−0.106), and smoking (p < 0.0001; CI 95% −0.122–−0.040) were significantly associated with decreased antibody concentrations. Female sex (p = 0.03; CI 95% 0.013–0.066) was associated with increased antibody concentrations. The third booster dose had a better effect on immunogenicity, with higher antibody concentrations among tested HCW. Heart disease, smoking, and other known risk factors were associated with decreased antibody concentrations.
LASER SCINTILLATION CAUSED BY TURBULENCE NEAR THE GROUND.

Abstract : Laser scintillation was measured for a horizontal optical path 500 m long and 1 m high for various conditions of horizontally homogeneous turbulence. Wind direction, average vertical distributions of wind speed and temperature, and, in some cases, turbulent fluctuations of wind velocity were measured simultaneously. The results of the measurements were analyzed in relation to a set of theoretical relationships derived by Tatarski (1961) for electromagnetic wave propagation in turbulent flow. Tatarski's derivation of the scintillation frequency spectrum (at a point in a plane perpendicular to the optical path) in relation to the three-dimensional spectral density of index of refraction inhomogeneities is summarized and interpreted in relation to its validity for various conditions of turbulence. Analysis of the spectral data showed that their characteristics were similar to Tatarski's theoretical spectrum if the divergence of the laser beam, the size of the receiver aperture, the intensity of scintillation and turbulence spectra were considered. (Author)
Design of Comparative Experiments: Exercises

Preface 1. Forward look 2. Unstructured experiments 3. Simple treatment structure 4. Blocking 5. Factorial treatment structure 6. Row-column designs 7. Experiments on people and animals 8. Small units inside large units 9. More about Latin squares 10. The calculus of factors 11. Incomplete-block designs 12. Factorial designs in incomplete blocks 13. Fractional factorial designs 14. Backward look Exercises Sources of examples, Questions and exercises Further reading Bibliography Index.
Genetic Evolution Analysis and Host Characteristics of Hantavirus in Yunnan Province, China

For a long time, the epidemic situation of hemorrhagic fever with renal syndrome (HFRS) caused by hantavirus (HV) in Yunnan Province of China has been relatively severe. The molecular epidemiology and host characteristics of HV in Yunnan Province are still not completely clear, and the systematic and long-term investigation of the epidemic area is very limited. In this study, a total of 488 murine-shaped animals were captured in the three regions of Mile City, Mangshi City and Lianghe County in Yunnan Province, and then the type of HV was identified by multiplex real-time RT-PCR and sequenced. The results indicate that 2.46% of the murine-shaped animal specimens were infected with HV. A new subtype of Seoul virus (SEOV) was found in the rare rat species Rattus nitidus in Lianghe County, and the two strains of this new subtype were named YNLH-K40 and YNLH-K53. Through the phylogenetic analysis of this new subtype, it is shown that this new subtype is very similar to the type S5 of SEOV, which is previously described as the main cause for the high incidence of HFRS in Longquan City, Zhejiang Province, China. This new subtype is highly likely to cause human infection and disease. Therefore, in addition to further promoting the improvement of the HV gene database and strengthening the discovery and monitoring of the host animals in Yunnan Province, more attention should be paid to the pathogenic potential of the newly discovered HV type.
Reinsurance: A Risk Management Instrument for Insurance Companies’ Profitability (A Case of Non-Life Insurance in Nigeria)

The study is carried out to determine reinsurance in place of a risk management instrument and its effect on the non-life insurance firms’ profitability in Nigeria, adopting ex-post-facto design, with multiple correlation and regression model to analyze the data obtained from Nigeria Insurers Association Digest report 2007 to 2018, which is indicating 12 years of study sample size. The findings of the study show that Premium Cession Ratio (PCR) and Net Retention Ratio (NRR) have positive effect on the profitability (ROA) of insurance companies but not significant with their coefficient values of (0.059880 and 0.006273) and their associated probability values of (0.2811 and 0.8880) respectively. However, the findings reveal that Reinsurance Dependence Cede Premium (RDC) and Reinsurance Commission to Shareholder’s Equity (RCS) have affected the profitability (ROA) of insurance business entities negatively and insignificantly with their values of coefficient (-0.266824 and -0.894553) and their corresponding p-value is (0.5149 and 0.3497) respectively.
Becoming a Grandparent in the Internet Age: An Integrative Model of Intergenerational Relationships within the Family and Grandparents’ Satisfaction with Life

ABSTRACT Becoming a grandparent is a significant transition to an important life role. Yet little is known about the diverse ways that grandparents interact with their young grandchildren, and how contact via new forms of communication might contribute to building and solidifying these relationships. The main objective of this study was to explore how grandparents use different forms of interaction, including information and communication technologies (ICTs), to interact with their grandchildren in the early years of the grandparental relationship. Results suggest that ICTs could play a role in supporting grandparent-grandchild relationship quality, acting as a complement to face-to-face contact.
Structural Changes in Metallic Glass-Forming Liquids on Cooling and Subsequent Vitrification in Relationship with Their Properties

The present review is related to the studies of structural changes observed in metallic glass-forming liquids on cooling and subsequent vitrification in terms of radial distribution function and its analogues. These structural changes are discussed in relationship with liquid’s properties, especially the relaxation time and viscosity. These changes are found to be directly responsible for liquid fragility: deviation of the temperature dependence of viscosity of a supercooled liquid from the Arrhenius equation through modification of the activation energy for viscous flow. Further studies of this phenomenon are necessary to provide direct mathematical correlation between the atomic structure and properties.
A Review on the Efficacy of Extraosseous Local Infiltration of Multimodal Drug Cocktail for Pain Management After Total Knee or Hip Arthroplasty

Total knee arthroplasty (TKA) patients express minimal comfort regarding postoperative pain management. The use of parenteral opioids or epidural analgesia may have unfavorable adverse impacts that interfere with quick healing and rehabilitation. It is uncertain if periarticular multimodal drug injections (PMDI) are effective at easing pain following total knee or total hip arthroplasty (THA). We conducted this study to assess the effectiveness of PMDI following TKA or THA. Articles were sourced using the following keywords on Pubmed, Google scholar, and the Web of Science: multimodal drug cocktail in total knee arthroplasty OR hip arthroplasty, periarticular injections AND multimodal drug cocktail, epidural versus periarticular injections AND pain management after total joint arthroplasty. After screening 438 articles and abstracts, 200 pertinent studies were found, of which a total of 10 articles were included in the study. From this review, we want to conclude that despite the various ways to address postoperative pain, there is no acknowledged gold standard for postoperative pain management following total joint arthroplasty. To reduce narcotic intake and prevent narcotic-related adverse reactions, multimodal techniques utilizing regional anesthetics appear to be on the rise such as periarticular injections, or patient-controlled analgesia with or without femoral nerve block. Even though the ideal duration and kind of medications are unclear, preoperative pain management or preemptive analgesia with anti-inflammatory drugs and opioid analgesics seem to be useful in lowering postoperative pain.
The Role of ctDNA in Gastric Cancer

Simple Summary DNA release from tumour cells (call circulating tumour DNA) into the blood stream can be found in patients with gastric cancer through a blood test call a liquid biopsy. This less invasive test can assess the genetic make-up of tumours to provide important information on the mechanisms of cancer development, identify mutations which can be targeted with drugs and could be used to screen for patients with gastric cancer. This article will review the current and future uses of liquid biopsies in gastric cancer. Abstract Circulating tumour DNA (ctDNA) has potential applications in gastric cancer (GC) with respect to screening, the detection of minimal residual disease (MRD) following curative surgery, and in the advanced disease setting for treatment decision making and therapeutic monitoring. It can provide a less invasive and convenient method to capture the tumoural genomic landscape compared to tissue-based next-generation DNA sequencing (NGS). In addition, ctDNA can potentially overcome the challenges of tumour heterogeneity seen with tissue-based NGS. Although the evidence for ctDNA in GC is evolving, its potential utility is far reaching and may shape the management of this disease in the future. This article will review the current and future applications of ctDNA in GC.
From Cytokine Storm to Cytokine Breeze: Did Lessons Learned from Immunopathogenesis Improve Immunomodulatory Treatment of Moderate-to-Severe COVID-19?

Complex immune response to infection has been highlighted, more than ever, during the COVID-19 pandemic. This review explores the immunomodulatory treatment of moderate-to-severe forms of this viral sepsis in the context of specific immunopathogenesis. Our objective is to analyze in detail the existing strategies for the use of immunomodulators in COVID-19. Immunomodulating therapy is very challenging; there are still underpowered or, in other ways, insufficient studies with inconclusive or conflicting results regarding a rationale for adding a second immunomodulatory drug to dexamethasone. Bearing in mind that a “cytokine storm” is not present in the majority of COVID-19 patients, it is to be expected that the path to the adequate choice of a second immunomodulatory drug is paved with uncertainty. Anakinra, a recombinant human IL-1 receptor antagonist, is a good choice in this setting. Yet, the latest update of the COVID-19 Treatment Guidelines Panel (31 May 2022) claims that there is insufficient evidence to recommend either for or against the use of anakinra for the treatment of COVID-19. EMA’s human medicines committee recommended extending the indication of anakinra to include treatment of COVID-19 in adult patients only recently (17 December 2021). It is obvious that this is still a work in progress, with few ongoing clinical trials. With over 6 million deaths from COVID-19, this is the right time to speed up this process. Our conclusion is that, during the course of COVID-19, the immune response is changing from the early phase to the late phase in individual patients, so immunomodulating therapy should be guided by individual responses at different time points.
Protein S-Acyl Transferase GhPAT27 Was Associated with Verticillium wilt Resistance in Cotton

Protein palmitoylation is an ability of the frame of the cell marker protein is one of the most notable reversible changes after translation. However, studies on protein palmitoylation in cotton have not yet been performed. In our current research, the PAT gene family was systematically identified and bioinformatically analyzed in G. arboreum, G. raimondii, G. barbadense and G. hirsutum, and 211 PAT genes were authenticated and classified into six subfamilies. Sixty-nine PAT genes were identified in upland cotton, mainly at the ends of its the 26 chromosomes of upland cotton. The majority of these genes are located in the nucleus of the plant. Gene structure analysis revealed that each member encodes a protein that which contains at least one DHHC structural domain. Cis-acting element analysis indicated that GhPATs genes are mainly involved in hormone production, light response and stress response. Gene expression pattern analysis indicated that most GhPATs genes were differentially expressed upon induction by pathogenic bacteria, drought, salt, hot and cold stresses, and some GhPATs could be induced by multiple abiotic stresses simultaneously. GhPATs genes showed different expression patterns in tissue-specific assays and were found to be preferentially expressed in roots, followed by expression in stems and leaves. Virus-induced gene silencing (VIGS) experiments showed that cotton was significantly less resistant to Verticillium dahliae when GhPAT27 was silenced. We conclude that the GhPAT27 gene, which mediates S-palmitoylation acetylation, may be involved in the regulation of upland cotton resistance to Verticillium wilt (VW). Overall, this work has provided a fundamental framework for understanding the latent capabilities of GhPATs and a solid foundation for molecular breeding and plant pathogen resistance in cotton.
Generation mean analysis for some heat tolerance and quantitative characters in bread wheat (Triticum aestivum L.)

Genetic analyses were performed for heat tolerance parameters viz. heat injury and chlorophyll stability index, grain protein content, grain yield and its components in bread wheat. Three crosses namely Raj 3765 x PBW 343, Raj 3765 x Raj 4037 and Raj 3765 x Raj 4083 of four parents were used for genetic analyses. The mean days to maturity were depicted as 112.59, 1000 grain weight (38.39 g) and grain yield (16.14 g), respectively. General means for grain protein content, heat injury and chlorophyll stability index were exhibited as 12.58, 44.02 and 14.32 per cents, respectively. A, B, C and D scaling tests revealed that additive-dominance model was inadequate for all the three crosses for days to maturity, two crosses for days to heading, and one cross for grain protein content, which indicated presence of non-allelic interactions. However, all the three crosses showed adequacy of additive-dominance model for grain yield and its components. Among digenic interaction dominance x dominance (l) and additive x additive (i) were more important as compared to additive x dominance (j) for most of the characters. Duplicate type of epistasis was exhibited for days to maturity in two crosses viz. Raj 3765 x PBW 343 and Raj 3765 x Raj 4037, while for grain protein content in one cross Raj 3765 x Raj 4037.
A Current and Common Cause of Secondary Spontaneous Pneumothorax: Covid- 19 Pneumonia

This study evaluated cases of spontaneous pneumothorax developing secondary to SARS-CoV-2 pneumonia. Sixteen cases presenting to our hospital due to spontaneous pneumothorax developing secondary to SARS-CoV-2 pneumonia between March 2020 and February 2020 were evaluated retrospectively. Ten patients (62.5%) were men, and six (37.5%) were women, with a mean age of 68 ± 20.3 years (range 18 - 90 years). Pneumothorax was in the right hemithorax in 11 cases (68.75%), in the left hemithorax in two (12.5%), and bilateral in three (17.75%). Pneumothorax developed during active SARS-CoV-2 pneumonia in all 16 cases (100%). No pneumothorax was detected following the healing of SARS-CoV-2 infection. Pneumothorax was observed while patients were not intubated in 15 cases (93.75%), but pneumothorax developed during mechanical ventilation in one case (6.25%). Tube thoracostomy was performed on all patients in treatment. Air leakage from the tube was observed in 14 cases (87.5%). The mean duration of tube thoracostomy was 18.3 ± 20.1 days (range 1 - 81 days). Pneumothorax resolved after treatment in seven cases (43.75%), while mortality occurred in nine (56.25%). Pneumothorax recurred after treatment in one case (6.25%). Pneumothorax is widely seen in the active period or after healing in cases infected with COVID-19. Aggressive treatment is generally required for this clinical manifestation with high mortality.