Miracle-dz/UniFAR-FTUs huggingface dataset:
Non‐psychotropic Cannabis sativa L. phytocomplex modulates microglial inflammatory response through CB2 receptors‐, endocannabinoids‐, and NF‐κB‐mediated signaling

Cannabis sativa L. is increasingly emerging for its protective role in modulating neuroinflammation, a complex process orchestrated among others by microglia, the resident immune cells of the central nervous system. Phytocannabinoids, especially cannabidiol (CBD), terpenes, and other constituents trigger several upstream and downstream microglial intracellular pathways. Here, we investigated the molecular mechanisms of a CBD‐ and terpenes‐enriched C. sativa extract (CSE) in an in vitro model of neuroinflammation. We evaluated the effect of CSE on the inflammatory response induced by exposure to lipopolysaccharide (LPS) in BV‐2 microglial cells, compared with CBD and β‐caryophyllene (CAR), CB2 receptors (CB2r) inverse and full agonist, respectively. The LPS‐induced upregulation of the pro‐inflammatory cytokines IL‐1β, IL‐6, and TNF‐α was significantly attenuated by CSE and only partially by CBD, whereas CAR was ineffective. In BV‐2 cells, these anti‐inflammatory effects exerted by CSE phytocomplex were only partially dependent on CB2r modulation and they were mediated by the regulation of enzymes responsible for the endocannabinoids metabolism, by the inhibition of reactive oxygen species release and the modulation of JNK/p38 cascade with consequent NF‐κB p65 nuclear translocation suppression. Our data suggest that C. sativa phytocomplex and its multitarget mechanism could represent a novel therapeutic strategy for neuroinflammatory‐related diseases.
Analytical variability and uncertainty in canine leukocyte ratios obtained with manual counts.

BACKGROUND This study aimed to determine the analytical imprecision of calculated neutrophil to lymphocyte (NLR) and lymphocyte to monocyte ratios generated from manual differential white blood cell (WBC) counts in peripheral blood smears, and to describe how to report the uncertainty around a single WBC ratio result. No information on the analytical imprecision of WBC ratios in dogs is available. METHODS Coefficient of variations (CVs) of paired readings of one operator on 105 smears (intraoperator variability) and of three operators on 301 smears (interoperator variability) were calculated. The interoperator agreement was examined with the Fleiss' kappa coefficient (κ). Observed total errors (TEos), expanded measurement of uncertainty (EMU) and reporting intervals (RIs) were also calculated. RESULTS Median CVs ranged from 3.14 to 28.28 (intraoperator) and from 5.39 to 53.85 (interoperator). No agreement among operators was found around the cut-offs. TEos were higher than allowable total errors in 32%-88% of smears. EMU ranged from 0.10 to 1.13. According to the RI, the calculated WBC ratios should be rounded to the nearest 10. CONCLUSION WBC ratios should be interpreted cautiously in dogs. The EMU should be reported to make the clinician aware of the uncertainty of these parameters. For example, an NLR result of 17 is needed to have high confidence that the result is above a cut-off of 6.
Exploring documentation in Person‐centred care: A content analysis of care plans

Abstract Background Person‐centred care is a growing imperative in healthcare, but the documentation of person‐centred care is challenging. According to the Gothenburg Framework of Person‐centred Care, care should be documented in continuously revised care plans and based on patients’ personally formulated goals and resources to secure a continuous partnership. Objectives This study aimed to examine care plans produced within a randomised controlled trial that tested a person‐centred care intervention in older people with acute coronary syndrome. Nurses with training in the theory and practice of person‐centred care had written the care plans. Methods We conducted a secondary analysis of care plans developed in a randomised controlled trial for assessing person‐centred care in patients with acute coronary syndrome (Myocardial Infarct [MI] or unstable angina pectoris). The study sample included 84 patients, with three care plans for each patient from inpatient (T1), outpatient (T2) and primary care (T3), that is, a total of 252 care plans. We conducted a descriptive quantitative content analysis of the care plans to examine the reported patients' life‐world and medical/health resources and goals. Results The analysis illustrates the differences and overlaps between life‐world and medical/health goals and resources. The documented goals and resources change over time: life‐world goals and resources decreased with time as medical/health goals and resources documentation increased. Conclusions This paper illustrates that in the setting of a randomised controlled trial, nurses with training in person‐centred care recorded fewer life‐world and more medical/health goals over time. Placing life‐world goals at the top of the goal hierarchy enables alignment with medical/health goals. Further research should explore whether the goals and resources documented in care plans accurately reflect patients' wishes as they transition along the care chain. Trial registration: Swedish registry, Researchweb.org, ID NR 65 791.
Prospective randomized trial of metal versus resorbable plates in surgical stabilization of rib fractures

Metal plates provide better initial alignment with no displacement over time as compared to resorbable plates in patients with traumatic rib fractures undergoing surgical stabilization. BACKGROUND Surgical stabilization of rib fractures has gained popularity as both metal and resorbable plates have been approved for fracture repair. Is there a difference between metal and resorbable plate rib fixation regarding rib fracture alignment, control of pain, and quality-of-life (QOL) scores (Rand SF-36 survey)? METHODS Eligible patients (pts) included 18 years or older with one or more of the following: flail chest, one or more bicortical displaced fractures (3–10), nondisplaced fractures with failure of medical management. Patients were randomized to either metal or resorbable plate fixation. Primary outcome was fracture alignment. Secondary outcomes were pain scores, opioid use, and QOL scores. RESULTS Thirty pts were randomized (15 metal/15 resorbable). Total ribs plated 167 (88 metal/79 resorbable). Patients with rib displacement at day of discharge (DOD) metal 0/14 (one pt died, not from plating) versus resorbable 9/15 or 60% (p = 0.001). Ribs displaced at DOD metal 0/88 versus resorbable 22/79 or 28% (p < 0.001), 48% in posterior location. Patients with increased rib displacement 3 months to 6 months: metal, 0/11 versus resorbable, 3/9 or 33% (p = 0.043). Ribs with increased displacement 3 months to 6 months metal 0 of 67 versus resorbable 6 of 49 or 12.2% (p < 0.004). Pain scores and narcotic use at postoperative Days 1, 2, 3, DOD, 2 weeks, 3 months and 6 months showed no statistically significant difference between groups. QOL scores were also similar at 3 months and 6 months. Trauma recidivism in outpatient period resulted in fracture of resorbable plates in two pts requiring a second surgery. CONCLUSION Metal plates provided better initial alignment with no displacement over time. Clinical outcomes were similar regarding pain, narcotic use, and QOL scores. Routine use of resorbable plates for posterior rib fractures is not warranted. Lateral repairs were technically most feasible for using resorbable plates but still resulted in significant displacement. Resorbable plates may not maintain rib alignment when exposed to subsequent injury. LEVEL OF EVIDENCE Therapeutic/Care Management; Level II
Pre-Operative Planning in Complex Deformities and Use of Patient-Specific UNiD™ Instrumentation

Study Design Review of current literature and authors experience. Objective Pre-operative planning is an integral part of complex spine surgery. With the advent of computer-assisted planning, multiple surgical plans can be evaluated utilizing alignment parameters, and the best plan for individual patients selected. However, the ability to evaluate and measure surgical correction goals intraoperatively are still limited. The use of patient-specific UNiD rods, created based on pre-operative plans, provided an initial tool for implementation of pre-operative plans in the operative setting. Methods A literature review for the use of patient-specific UNiD rods in thoracolumbar spine complex surgery was performed. The articles were selected and reviewed for the initial experience/outcomes of these techniques. Further, the initial experience of the authors at The University of Colorado is described. Results The use of UNiD patient-specific rods, in combination with pre-operative planning has been shown to provide a higher rate of patients with spinopelvic alignment parameters within currently accepted ranges. This includes improvement of sagittal vertical axis (SVA) < 50 mm and pelvic incidence (PI)–lumbar lordosis (LL) = ± 10°. Multiple authors have shown improvement in pelvic tilt to age adjusted values but note continued difficulties in obtaining correction goals. Conclusions The use of pre-operative planning software and UNiD patient-specific rods has been shown to improve surgeon’s ability to achieve spinopelvic alignment parameters, specifically SVA and PI-LL, along with other possible benefits. Further research is needed regarding long-term value of the technology.
Effective psychological therapy for PTSD changes the dynamics of specific large‐scale brain networks

In posttraumatic stress disorder (PTSD), re‐experiencing of the trauma is a hallmark symptom proposed to emerge from a de‐contextualised trauma memory. Cognitive therapy for PTSD (CT‐PTSD) addresses this de‐contextualisation through different strategies. At the brain level, recent research suggests that the dynamics of specific large‐scale brain networks play an essential role in both the healthy response to a threatening situation and the development of PTSD. However, very little is known about how these dynamics are altered in the disorder and rebalanced after treatment and successful recovery. Using a data‐driven approach and fMRI, we detected recurring large‐scale brain functional states with high temporal precision in a population of healthy trauma‐exposed and PTSD participants before and after successful CT‐PTSD. We estimated the total amount of time that each participant spent on each of the states while being exposed to trauma‐related and neutral pictures. We found that PTSD participants spent less time on two default mode subnetworks involved in different forms of self‐referential processing in contrast to PTSD participants after CT‐PTSD (mtDMN+ and dmDMN+) and healthy trauma‐exposed controls (only mtDMN+). Furthermore, re‐experiencing severity was related to decreased time spent on the default mode subnetwork involved in contextualised retrieval of autobiographical memories, and increased time spent on the salience and visual networks. Overall, our results support the hypothesis that PTSD involves an imbalance in the dynamics of specific large‐scale brain network states involved in self‐referential processes and threat detection, and suggest that successful CT‐PTSD might rebalance this dynamic aspect of brain function.
Online Measurement Usage for Predicting Water Age from Tracer Tests to Validate a Hydraulic Model

The tracer tests were performed using two approaches in the drinking water distribution network (1374 km) of Riga city, Latvia with the goal to verify and validate a hydraulic model build up in Epanet 2.0. The water is supplied for 700 000 inhabitants with average daily water demand of 1500 l/s from six different water sources. The each water source has a different electric conductivity (EC) value with the lowest numbers for deep groundwater 272±25 µS/cm (25 O C) and the highest for surface and artificially recharged groundwater 468±100 and 784±18 µS/cm, respectively. The significant difference for EC in sources was used as a natural tracer. The first measurement approach is based on field works during shut-down or start-up of WTP due to monthly working regime or yearly drainage of clear water tanks at stations located in the north-east of city. In total more than 20 locations in distribution network were used for measurements of EC from hydrants and/or at taps in buildings applying online conductivity meters 3400sc™ (Dr. Bruno Lange GmbH&Co, Germany) and condu::lyser™ (s::can Mestechnik GmbH, Austria) in predictable time interval. The second approach of tracer measurements was based on EC measurements in network within close distance to track minor changes of EC due to production/consumption ratio. The hydraulic model of Riga city was validated in 8 nodes with the maximum age of 25:40 hours, while the distal nodes in model accounts for 60 hours. Results showed that in order to measure correct changes of trace element it is necessary to do it very close to transient pipes with high flow. The measurements of tracer near reservoirs in network are unpredictable due to proper mixing of water. The approach of measurements with two online instruments in close distance (up to 5 km) to track minor changes of EC can be useful for validation of hydraulic models. However, the statistical analysis (z-test) of this method showed significant difference between mean values of measurements which can be explained with too many uncertainties (cross-connections of parallel lines, loops) for water mixing in city with population of 700 000 inhabitants.
Effect of cold storage on development and demographic parameters of Scolothrips longicornis fed on two-spotted spider mite

Abstract Cold storage effects on the female predatory thrips Scolothrips longicornis Priesner (Thysanoptera: Thripidae) fed on Tetranychus urticae Koch eggs for different time periods (5, 10, 20 and, 30 days) at 5 or 25 °C (control) on development and life table parameters were examined. Female adult duration and female total longevity were not influenced by the period of cold storage. The minimum mean female total longevity (28.1 ± 0.32 day) was observed in the 30-day treatment. The oviposition period decreased with increasing cold storage duration. All life table parameters of S. longicornis demonstrated a significant difference across treatments. Our results demonstrated that the female adult of S. longicornis could be maintained at 5 °C for 5-days with minimal reduction in the life-history process. This would be beneficial in the storage and mass production of S. longicornis in biocontrol programs involving T. urticae.
Role of dietary quality and diversity on overweight and obesity among women of reproductive age in Tanzania

This study aimed to examine associations of dietary quality and diversity among reproductive-aged women with overweight and obesity. We conducted a cross-sectional study in the Health and Demographic Surveillance System of the Dar es Salaam Urban Cohort Study (DUCS) in Tanzania. A random sample of 1004 non-pregnant women was selected from the DUCS population database and interviewed about dietary information using the FFQ. Women were aged 30.2 (±8.1) years; 27.8% were overweight and 22.6% were obese. All 1004 women in the study consumed starchy staple foods. Of all the women studied, 10.5%, 1.7% and 3.8% consumed vitamin A rich dark green vegetables, nuts and seeds, and beans and peas, respectively. Compared with women in the lowest quintile of Prime Dietary Quality Score (PDQS), those who were in the highest quintile were significantly less likely to be overweight or obese (Adjusted Prevalence Ratio (APR) = 0.76, 95%CI: 0.62, 0.89) (F for trend = 0.029). Dietary diversity score (DDS) was not significantly associated with overweight and obesity. Risk factors included the highest consumption of animal foods (APR = 2.81, 95% CI: 1.51–3.51) and fast food (APR = 2.57, 95% CI: 1.24–4.34). Consumption of legumes and whole grains was associated with lower risk (APR = 0.59; 95% CI: 0.38–0.2). Dietary quality is an important predictor of overweight and obesity among women of reproductive age. Nutrition interventions may be warranted to support women of reproductive age to enter pregnancy with healthier weight to prevent adverse pregnancy outcomes and future risk of chronic diseases.
Mate evaluation theory.

There are two unresolved puzzles in the literature examining how people evaluate mates (i.e., prospective or current romantic/sexual partners). First, compatibility is theoretically crucial, but attempts to explain why certain perceivers are compatible with certain targets have revealed small effects. Second, features of partners (e.g., personality, consensually rated attributes) affect perceivers' evaluations strongly in initial-attraction contexts but weakly in established relationships. Mate Evaluation Theory (MET) addresses these puzzles, beginning with the Social Relations Model postulate that all evaluative constructs (e.g., attraction, relationship satisfaction) consist of target, perceiver, and relationship variance. MET then explains how people draw evaluations from mates' attributes using four information sources: (a) shared evolved mechanisms and cultural scripts (common lens, which produces target variance); (b) individual differences that affect how a perceiver views all targets (perceiver lens, which produces perceiver variance); (c) individual differences that affect how a perceiver views some targets, depending on the targets' features (feature lens, which produces some relationship variance); and (d) narratives about and idiosyncratic reactions to one particular target (target-specific lens, which produces most relationship variance). These two distinct sources of relationship variance (i.e., feature vs. target-specific) address Puzzle #1: Previous attempts to explain compatibility used feature lens information, but relationship variance likely derives primarily from the (understudied) target-specific lens. MET also addresses Puzzle #2 by suggesting that repeated interaction causes the target-specific lens to expand, which reduces perceivers' use of the common lens. We conclude with new predictions and implications at the intersection of the human-mating and person-perception literatures. (PsycInfo Database Record (c) 2022 APA, all rights reserved).
Assessment of clinical characteristics of cardiac amyloidosis as a potential underlying etiology in patients diagnosed with heart failure with preserved ejection fraction.

BACKGROUND Heart failure with preserved ejection fraction (HFpEF) is a heterogeneous clinical syndrome. Transthyretin cardiac amyloidosis (CA) is an underdiagnosed cause of HFpEF. Red flags are extremely useful for suspecting CA. AIMS We aimed to evaluate frequency of cardiac and extracardiac clinical characteristics of CA in HFpEF patients based on red flags. METHODS Baseline characteristics were recorded during admission. Electrocardiogram and echocardiography were performed. All patients were examined in terms of RF. Cardiac scintigraphy was performed in 85 patients. RESULTS The mean (standard deviation [SD]) age of the study group was 67.9 (9.8) years, and 52 (61.2%) were female. At least 1 red flag was observed in 67% of HFpEF patients. Only 4 of the patients had more than 3 red flags. The mean number of red flags in a patient with HFpEF was 1.3. Extracardiac clinical red flags were observed in only 9 (10.5%) patients. Cardiac clinical red flags were extremely rare. An electrocardiographic red flag was detected in 2 out of 10 patients and an echocardiographic red flag in 4 out of 10 patients with HFpEF. Scintigraphy showed that 17.6% of the all patients have had a grade 2 or 3 cardiac uptake. The patients with wild-type transthyretin CA had twice as many red flags as those without. CONCLUSION The results of the study showed that; patients diagnosed with HFpEF had an average of 1.3 red flags suggestive of CA. In real life; extracardiac red flags are rare, electrocardiographic and echocardiographic red flags are more common in patients with HFpEF.
Inhaling salbutamol may decrease time to exhaustion in some contexts of heavy endurance performances

ABSTRACT Purpose: To study the effect of inhaling a beta-agonist (salbutamol) compared to placebo on skiing and cycling performance in well-trained elite athletes. Methods: Three different exercise protocols were used, all with a cross-over double blind placebo-controlled design. Participants inhaled 800 µg salbutamol or a placebo prior to the test, which was repeated on a following day with the participants inhaling the other substance. Fifteen junior elite skiers performed four free-style high intensity sprints (1100 m/work time 3.5–4.5 min). Twelve elite cyclists carried out a short cycling protocol, starting with two 5 min submaximal workloads followed by a maximal intermittent performance test to exhaustion. Another 12 elite cyclists performed the maximal intermittent performance test to exhaustion after a 150 min long submaximal cycling protocol. Results: Group mean time for the ski sprints increased, with no difference between treatment groups. In the short cycling protocol time to exhaustion was 9.1% (95% CI 52–161) lower after inhaling salbutamol compared to placebo and in the long cycling protocol time to exhaustion was 9.1% (95% CI – 121–267) lower after inhaling salbutamol compared to placebo. Blood lactate, heart rate and ventilation increased during submaximal exercise with salbutamol compared to placebo in the short cycling protocol (p < .05). Conclusion: This study could not confirm any positive performance effects from inhaling 800 µg salbutamol compared to placebo in skiing and high-intensity intermittent cycling performance. Instead, time to exhaustion in the maximal intermittent performance test was lower in both cycling protocols. Highlights There was no difference in performance time between salbutamol and placebo treatment in real-life applicable repeated ski sprints. Time to exhaustion in the maximal intermittent performance test was 9.1% lower after inhaling salbutamol compared to placebo, both when performed after 10 and 150 min of submaximal cycling.
An analysis of 77 cases of pancreatic injuries at a level one trauma center: Outcomes of conservative and surgical treatments

Backgrounds/Aims Traumatic pancreatic injury (TPI) is rare as an isolated injury. There is a trend to perform conservative treatment even in patients with complete duct dissection and successful treatment. This study reviewed our 20 years of experience in the management of TPI and assessed patient outcomes according to age group and treatment strategy. Methods A retrospective analysis of patients diagnosed and treated with TPI at a level-I trauma center from 2000-2019. Patients were divided into two groups: adults and pediatrics. Conservative treatment cases were subjected to subgroup analysis. Level of evidence: IV. Results Of a total of 77 patients, the mean age was 24.89 ± 15.88 years. Fifty-six (72.7%) patients had blunt trauma with motor vehicle accident. Blunt trauma was the predominant mechanism in 42 (54.5%) patients. Overall, 38 (49.4%) cases had grade I or II injury, 24 (31.2%) had grade III injury, and 15 (19.5%) had grade IV injury. A total of 30 cases had non-operative management (NOM). Successful NOM was observed in 16 (20.8%) cases, including eight (32.0%) pediatric cases and eight (15.4%) adult cases. Higher American association for the surgery of trauma (AAST) grade of injury was associated with NOM failure (16.7% for grade I/II, 100% for grade III, and 66.7% for grade IV injury; p = 0.001). An independent factor for NOM failure was female sex (69.2% in females vs. 29.4% in males; p = 0.03). Conclusions High AAST grade TPI is associated with a high rate of NOM failure in both pediatric and adults.