Miracle-dz/UniFAR-FTUs huggingface dataset:
Human Lung Conventional Dendritic Cells Orchestrate Lymphoid Neogenesis During COPD.

RATIONALE Emerging evidence supports a crucial role for tertiary lymphoid organs (TLOs) in chronic obstructive pulmonary disease (COPD) progression. However, mechanisms of immune cell activation leading to TLO in COPD remain to be defined. OBJECTIVES To examine the role of lung dendritic cells (DC) in T follicular helper (Tfh)-cell induction, a T-cell subset critically implicated in lymphoid organ formation, in COPD. METHODS Myeloid cell heterogeneity and phenotype was studied in an unbiased manner via single-cell RNA sequencing on HLA-DR+ cells sorted from human lungs. The in vitro capability of FACS-sorted DC-subsets of control and COPD lungs to polarize IL-21+CXCL13+ Tfh-like cells was measured. In situ imaging analysis was performed on COPD stage IV GOLD lungs with TLO. MEASUREMENTS AND MAIN RESULTS ScRNAseq analysis revealed a high level of heterogeneity among human lung myeloid cells. Among these, cDC2 showed increased induction of IL-21+CXCL13+ Tfh-like cells. Importantly, the capacity to induce IL-21+ Tfh-like cells was higher in cDC2s from COPD patients compared with control patients. Increased Tfh-induction by COPD cDC2 correlated with increased presence of Tfh-like cells in COPD lungs as compared to controls, and cDC2 co-localized with Tfh-like cells in TLOs of COPD. Mechanistically, cDC2 exhibited a unique migratory signature and (transcriptional) expression of several pathways and genes related to DC-induced Tfh-priming. Importantly, blocking the co-stimulatory OX40L-OX40 axis reduced Tfh-induction by control lung cDC2. CONCLUSIONS In COPD lung, we found lung EBI2+ OX-40L-expressing cDC2 that induces IL-21+ Tfh-like cells, suggesting an involvement of these cells in TLO formation.
Impacts of Drought on Maize and Soybean Production in Northeast China During the Past Five Decades

Climate change has a distinct impact on agriculture in China, particularly in the northeast, a key agriculture area sensitive to extreme hydroclimate events. Using monthly climate and agriculture data, the influence of drought on maize and soybean yields—two of the main crops in the region—in northeast China since 1961 to 2017 were investigated. The results showed that the temperature in the growing season increased by 1.0 °C from the period 1998–2017 to the period 1961–1980, while the annual precipitation decreased slightly. However, precipitation trends varied throughout the growing season (May–September), increasing slightly in May and June, but decreasing in July, August and September, associated with the weakening of the East Asian summer monsoon. Consequently, the annual and growing season drought frequency increased by 15%, and 25%, respectively, in the period 1998–2017 relative to the period 1961–1980. The highest drought frequency (55%) was observed in September. At the same time, the drought intensity during the growing season increased by 7.8%. The increasing frequency and intensity of drought had negative influences on the two crops. During moderate drought years in the period 1961–2017, 3.2% and 10.4% of the provincial maize and soybean yields were lost, respectively. However, during more severe drought years, losses doubled for soybean (21.8%), but increased more than four-fold for maize (14.0%). Moreover, in comparison to the period 1961–1980, a higher proportion of the yields were lost in the period 1998–2017, particularly for maize, which increased by 15% (increase for soybean was 2.4%). This change largely depends on increasing droughts in August and September, when both crops are in their filling stages. The impact of drought on maize and soybean production was different during different growth stages, where a strong relationship was noted between drought and yield loss of soybean in its filling stage. Given the sensitivity of maize and soybean yields in northeast China to drought, and the observed production trends, climate change will likely have significant negative impacts on productivity in the future.
Carbon Monoxide, a Retrograde Messenger Generated in Postsynaptic Mushroom Body Neurons, Evokes Noncanonical Dopamine Release

Dopaminergic neurons innervate extensive areas of the brain and release dopamine (DA) onto a wide range of target neurons. However, DA release is also precisely regulated. In Drosophila melanogaster brain explant preparations, DA is released specifically onto α3/α′3 compartments of mushroom body (MB) neurons that have been coincidentally activated by cholinergic and glutamatergic inputs. The mechanism for this precise release has been unclear. Here we found that coincidentally activated MB neurons generate carbon monoxide (CO), which functions as a retrograde signal evoking local DA release from presynaptic terminals. CO production depends on activity of heme oxygenase in postsynaptic MB neurons, and CO-evoked DA release requires Ca2+ efflux through ryanodine receptors in DA terminals. CO is only produced in MB areas receiving coincident activation, and removal of CO using scavengers blocks DA release. We propose that DA neurons use two distinct modes of transmission to produce global and local DA signaling. SIGNIFICANCE STATEMENT Dopamine (DA) is needed for various higher brain functions, including memory formation. However, DA neurons form extensive synaptic connections, while memory formation requires highly specific and localized DA release. Here we identify a mechanism through which DA release from presynaptic terminals is controlled by postsynaptic activity. Postsynaptic neurons activated by cholinergic and glutamatergic inputs generate carbon monoxide, which acts as a retrograde messenger inducing presynaptic DA release. Released DA is required for memory-associated plasticity. Our work identifies a novel mechanism that restricts DA release to the specific postsynaptic sites that require DA during memory formation.
Evaluating the Demand for Integrative Medicine Practices in Breast and Gynecological Cancer Patients

Introduction: Integrative medicine is becoming increasingly relevant to modern oncology. The present study aims to assess the demand for integrative therapeutic approaches including complementary and alternative medicine (CAM), body and mind practices, nutritional counseling, and psychological assistance among breast and gynecological cancer patients. Methods: From April to July 2017, a pseudo-anonymous questionnaire was distributed to cancer patients at the Department of Gynecology and Obstetrics, Technical University of Munich. Patterns of CAM use and demand for integrative health approaches were investigated by generating descriptive statistics. Results: 182 (90%) of 203 patients participated in the survey. 59% indicated the use of CAM practices in general, and 41% specifically in relation to their oncological disease. Most frequently used modalities were herbal supplements (32%), homeopathy (29%), vitamins (28%), and selenium (21%). Integrative health approaches that patients would have desired alongside conventional oncological treatment were CAM (54%), relaxation therapies (44%), and dietary counseling (43%). Conclusion: There is a considerable demand for integrative medicine among breast and gynecological cancer patients. Our results underline the need for the implementation of evidence-based integrative treatments in cancer care in order to meet the standards of modern oncology.
Dentists’ Awareness, Perception, and Attitude Regarding COVID-19 and Infection Control: Cross-Sectional Study Among Jordanian Dentists

Background Despite the availability of prevention guidelines and recommendations on infection control, many dental practices lack the minimum requirements for infection control. Objective This study aimed to assess the level of awareness, perception, and attitude regarding the coronavirus disease (COVID-19) and infection control among Jordanian dentists. Methods The study population consisted of dentists who worked in private clinics, hospitals, and health centers in Jordan. An online questionnaire was sent to a sample of Jordanian dentists in March 2020. The questionnaire was comprised of a series of questions about dentists’ demographic characteristics; their awareness of the incubation period, the symptoms of the disease, mode of transmission of COVID-19 and infection control measures for preventing COVID-19; and their attitude toward treating patients with COVID-19. Results This study included a total of 368 dentists aged 22-73 years (mean 32.9 years, SD 10.6 years). A total of 112 (30.4%) dentists had completed a master or residency program in dentistry, 195 (53.0%) had received training in infection control in dentistry, and 28 (7.6%) had attended training or lectures regarding COVID-19. A total of 133 (36.1%) dentists reported that the incubation period is 1-14 days. The majority of dentists were aware of COVID-19 symptoms and ways of identifying patients at risk of having COVID-19, were able to correctly report known modes of transmission, and were aware of measures for preventing COVID-19 transmission in dental clinics. A total of 275 (74.7%) believed that it was necessary to ask patients to sit far from each other, wear masks while in the waiting room, and wash hands before getting in the dental chair to decrease disease transmission. Conclusions Jordanian dentists were aware of COVID-19 symptoms, mode of transmission, and infection controls and measures in dental clinics. However, dentists had limited comprehension of the extra precautionary measures that protect the dental staff and other patients from COVID-19. National and international guidelines should be sent by the regional and national dental associations to all registered dentists during a crisis, including the COVID-19 pandemic, to make sure that dentists are well informed and aware of best practices and recommended disease management approaches.
Comparative Proteome Analyses of Ureteropelvic Junction Obstruction and Surrounding Ureteral Tissue

Ureteropelvic junction (UPJ) obstruction is a common problem in children, but its etiology remains unclear. In this study, the proteome profiles of the obstructed segment and its surrounding distal and proximal parts were comparatively evaluated. Twelve children younger than 2 years of age with unilateral intrinsic UPJ obstruction were included. The excised operational tissue was divided into three parts immediately after resection: the obstructed part (Obst), the distal normal ureteral part (Dist), and the proximal part of the obstructed segment (Prox). Proteins extracted from the tissue samples were subjected to two-dimensional gel electrophoresis analysis to identify differentially regulated proteins. Spot analysis revealed that four proteins, namely tropomyosin beta and alpha-1 chains, actin and desmin, were upregulated in Obst in comparison to Dist. A similar analysis between Obst and Prox showed that heat shock protein beta-1 and carbonic anhydrase-1 were upregulated in Obst, while tropomyosin alpha 3 chain and ATP synthase beta were upregulated in Prox. The last comparative analysis between Dist and Prox revealed upregulation of annexin-A5 and annexin-A1 in Dist and vimentin, mitochondrial ATP synthase subunit-beta, peroxiredoxin-2, and apolipoprotein-A1 in Prox. Bioinformatics analysis using the STRING server indicated that the differentially regulated proteins, altogether, point to the changes occurring in muscle filament sliding pathway. When regulations occurring in each group were mutually compared, a change in lipase inhibition activity was detected by STRING. This is the first study scrutinizing changes occurring in protein profiles in UPJ.
OP80 Effectiveness of european fans in training (EuroFIT): randomised controlled trial in england, portugal, the netherlands and norway

Background Despite widespread knowledge about the risks of unhealthy lifestyles, men can be reluctant to engage in lifestyle change programmes. Building on the success of the Scottish Football Fans in Training, we developed EuroFIT, a men-only, group-based lifestyle-change program to improve physical activity and reduce sitting time. Our aim was to investigate whether EuroFIT can help men aged 30–65 years with a self-reported BMI ≥27 kg/m2 to increase physical activity and decrease sitting time 12 months after baseline. Methods We conducted a pragmatic, two-arm, randomised controlled trial in 15 football clubs in the Netherlands, Norway, Portugal and the UK (England). We measured participants at baseline, post-program and 12 months after baseline. Primary outcomes were objectively assessed changes in total physical activity (steps per day) and total sedentary time (minutes per day spent sitting). Secondary outcomes include self-reported food intake, weight, resting systolic and diastolic blood pressure and cardio-metabolic blood biomarkers. We conducted linear mixed effects regression analyses, including random effects for country and football club, and fixed effects for study group and baseline measurement. Results 560 men were allocated to the intervention and objective measurements were achieved for 451 (81%) at 12 months; 553 were allocated to the control and objective measurements were achieved for 470 (83%). At 12 months, the mean difference in step count, adjusted for baseline steps and club, was 678 steps (308, 1048) in favour of the intervention (p<0.001); the mean difference in time spent sitting was 1.7 min (−10.4, 13.8) (p=0.78). We also found significicant improvements in self-reported food intake, weight, resting blood pressure and some cardio-metabolic blood biomakers in favour of the intervention. Seven serious adverse events were reported. Of these, 5 were assessed as likely to be associated with EuroFIT participation. Discussion Participation in EuroFIT led to modest improvement in physical activity but not sedentary time at 12 months. Public health messages to be more physically active and eat well are now widely understood but the ‘sit less’ message is newer and less is known about how to achieve it. In this context coaches and participants may have found it difficult to disentangle advice about sitting less from advice about being more physical activity. Differences in outcomes between FFIT and EuroFIT will be discussed. Authorship is on behalf of the EuroFIT consortium.
A meta-analysis of the vascular endothelial growth factor polymorphisms associated with the risk of pre-eclampsia

Abstract Backgroud: Pre-eclampsia (PE) is a common pregnancy-induced hypertension disease. Some case–control studies reported the association between vascular endothelial growth factor (VEGF) gene polymorphisms (rs3025039, rs2010963) and PE risk. However, these associations were inconsistent in several studies. Therefore, we conducted this meta-analysis to assess the role of VEGF gene polymorphisms in PE more precisely. Methods: Eligible studies were searched in PubMed, Embase, Web of Science and Chinese (Chinese National Knowledge Infrastructure (CNKI) and WanFang) databases. Statistical analyses were performed by Stata 12.0 software. Odds ratio (OR) and 95% confidence interval (CI) were used to assess the strength of the association. In addition, subgroup analyses, sensitive analyses and publication bias analyses were performed to further assess this meta-analysis. Results: In total, 21 studies were included in the meta-analysis covering 2018 cases and 2632 controls. There were significant associations between VEGF polymorphisms (rs3025039, rs2010963) and PE risk in the overall populations. In the subgroup analyses, we found that rs3025039 polymorphism was associated with the increased risk of PE among Chinese. As for rs2010963 polymorphism, a significant association was observed in subgroup of Caucasian. Conclusion: The present study suggested that the two VEGF gene polymorphisms (rs3025039, rs2010963) are associated with increased risk of PE in different ethnic groups, which means that the targets may be useful genetic markers for early prediction of PE.
The Role of Family Relationships in Eating Disorders in Adolescents: A Narrative Review

Background: Adolescents’ eating disorders have been explored through various conceptual and empirical models. Only recently, scientific literature in this area has more specifically investigated the role of relationships, with particular attention to family functioning. Objective: This paper reviews family relationships aspects of eating disorders in adolescence. Methods: A narrative literature review of relational issues in adolescents’ eating disorders was performed. Results: Empirical evidence of family relationships in adolescents’ eating disorders confirms the relevance of relational aspects in the development and maintenance of the pathology. In particular, the contribution of the relational-systemic approach is wide, suggesting the need to refer to the family context for a better understanding of adolescents’ sufferance. Additionally, the empirical contributions from the conceptual model of Developmental Psychopathology, highlighting the importance of risk and protection factors in family relationships, provides knowledge about the phenomenon of adolescents’ eating disorders in terms of complexity. Conclusions: An integrated relational model aimed to explore adolescents’ eating disorders is worthy of investigation to accomplish specific program of intervention.
The Cytomegalovirus Protein Kinase pUL97: Host Interactions, Regulatory Mechanisms and Antiviral Drug Targeting

Human cytomegalovirus (HCMV) expresses a variety of viral regulatory proteins that undergo close interaction with host factors including viral-cellular multiprotein complexes. The HCMV protein kinase pUL97 represents a viral cyclin-dependent kinase ortholog (vCDK) that determines the efficiency of HCMV replication via phosphorylation of viral and cellular substrates. A hierarchy of functional importance of individual pUL97-mediated phosphorylation events has been discussed; however, the most pronounced pUL97-dependent phenotype could be assigned to viral nuclear egress, as illustrated by deletion of the UL97 gene or pharmacological pUL97 inhibition. Despite earlier data pointing to a cyclin-independent functionality, experimental evidence increasingly emphasized the role of pUL97-cyclin complexes. Consequently, the knowledge about pUL97 involvement in host interaction, viral nuclear egress and additional replicative steps led to the postulation of pUL97 as an antiviral target. Indeed, validation experiments in vitro and in vivo confirmed the sustainability of this approach. Consequently, current investigations of pUL97 in antiviral treatment go beyond the known pUL97-mediated ganciclovir prodrug activation and henceforward include pUL97-specific kinase inhibitors. Among a number of interesting small molecules analyzed in experimental and preclinical stages, maribavir is presently investigated in clinical studies and, in the near future, might represent a first kinase inhibitor applied in the field of antiviral therapy.
Is fatty acid composition of breast milk different in various populations? A systematic review and meta-analysis

Abstract This systematic review and meta-analysis aims to systematically review the fatty acid (FA) composition of breast milk in different populations. We systematically searched the Medline via PubMed, Scopus and Clarivate till July 2019. We initially retrieved 433 articles. Having removed duplicates, with initial searching of title and abstracts, 317 papers were excluded and 80 remained. A meta-analysis of 80 cross-sectional, cohort studies, including 8793 subjects, was conducted. Meta-regression analysis indicated that factors including maternal age (p-values of SFA: 0.03, omega-3: 0.01, trans fats:<0.001, total fatty acids: 0.02), year of conducting study (p-values of SFA: <0.001, MUFA: 0.02, omega-6: 0.04,trans fats: 0.04) and sample size (p-values of SFA: 0.03, PUFA: 0.01, omega-6: 0.01, omega-3:<0.001) contributed to the heterogeneity of meta-analysis. This study indicates the large variation of FA content in maternal milk across different populations. Special concern is necessary for both the quality and total amount of fat intake of pregnant and lactating mothers.
Robust Outlier-Adaptive Filtering for Vision-Aided Inertial Navigation

With the advent of unmanned aerial vehicles (UAVs), a major area of interest in the research field of UAVs has been vision-aided inertial navigation systems (V-INS). In the front-end of V-INS, image processing extracts information about the surrounding environment and determines features or points of interest. With the extracted vision data and inertial measurement unit (IMU) dead reckoning, the most widely used algorithm for estimating vehicle and feature states in the back-end of V-INS is an extended Kalman filter (EKF). An important assumption of the EKF is Gaussian white noise. In fact, measurement outliers that arise in various realistic conditions are often non-Gaussian. A lack of compensation for unknown noise parameters often leads to a serious impact on the reliability and robustness of these navigation systems. To compensate for uncertainties of the outliers, we require modified versions of the estimator or the incorporation of other techniques into the filter. The main purpose of this paper is to develop accurate and robust V-INS for UAVs, in particular, those for situations pertaining to such unknown outliers. Feature correspondence in image processing front-end rejects vision outliers, and then a statistic test in filtering back-end detects the remaining outliers of the vision data. For frequent outliers occurrence, variational approximation for Bayesian inference derives a way to compute the optimal noise precision matrices of the measurement outliers. The overall process of outlier removal and adaptation is referred to here as “outlier-adaptive filtering”. Even though almost all approaches of V-INS remove outliers by some method, few researchers have treated outlier adaptation in V-INS in much detail. Here, results from flight datasets validate the improved accuracy of V-INS employing the proposed outlier-adaptive filtering framework.
Detecting the Effects of the Glucocorticoid Dexamethasone on Primary Human Skeletal Muscle Cells—Differences to the Murine Cell Line

Skeletal muscle atrophy is characterized by a decrease in muscle fiber size as a result of a decreased protein synthesis, which leads to degradation of contractile muscle fibers. It can occur after denervation and immobilization, and glucocorticoids (GCs) may also increase protein breakdown contributing to the loss of muscle mass and myofibrillar proteins. GCs are already used in vitro to induce atrophic conditions, but until now no studies with primary human skeletal muscle existed. Therefore, this study deals with the effects of the GC dexamethasone (dex) on primary human myoblasts and myotubes. After incubation with 1, 10, and 100 µM dex for 48 and 72 h, gene and protein expression analyses were performed by qPCR and Western blot. Foxo, MuRF-1, and MAFbx were significantly upregulated by dex, and there was increased gene expression of myogenic markers. However, prolonged incubation periods demonstrated no Myosin protein degradation, but an increase of MuRF-1 expression. In conclusion, applying dex did not only differently affect primary human myoblasts and myotubes, as differences were also observed when compared to murine cells. Based on our findings, studies using cell lines or animal cells should be interpreted with caution as signaling transduction and functional behavior might differ in diverse species.