Miracle-dz/UniFAR-FTUs huggingface dataset:
Structure, ooparasitoid spectrum and rate of parasitism of egg‐batches of Thaumetopoea pityocampa (Den. & Schiff.) (Lep., Thaumetopoeidae) in Greece 1 2

In the years 1988/89, 222 egg‐batches of the pine processionary caterpillar Thaumetopoea pityocampa (Den. & Schiff.) collected in Greece were studied in the laboratory for one year. Data on structure and size of the batches as well as a method rendering quite accurate estimates of the number of eggs per batch are presented. Altogether 10 871 parasitoids belonging to 6 species of Chalcidoidea emerged from the samples. The three most abundant species were Eutetrastichus servadeii (Dom.), Ooencyrtus pityocampae Mercet and Eutetrastichus sp. near servadeii. In the samples collected on Kassandra (KI) and on the Peloponnes (P) in autumn 1988 shortly after oviposition, the number of emerging primary parasitoids was largely related to the duration of field exposure of the batches. Caterpillars hatched from 67% of the eggs of these sample units. In egg‐batches collected on Kassandra after 7 months of exposure in the field (KII) a much greater parasitoid impact was observed. Not only did they yield more adult parasitoids throughout the first year; a striking difference was the much higher proportion of eggs without hatching or emergence holes, a majority of which showed signs of parasitoid attack. Only 4% of the eggs appeared to be sterile after one year. Host mortality in the KII egg‐batches was calculated to be 63%.
The difficulty of estimating population densities of nocturnal forest mammals from transect counts of animals

Many nocturnal small- to medium-sized forest mammals can be surveyed only by directly sighting or hearing each individual. This is particularly so in tropical regions. Signs (footprints, dung scratch-marks, etc.) are often not identifiable to species (similar species frequently occur together); villagers' identifications are rarely clear enough for research purposes, and the provenance of specimens in markets can rarely be established to satisfactory precision. Assessments of animal status from brief surveys are becoming increasingly important for many conservation purposes. A repeatable methodology for field assessment of the status of nocturnal mammals is needed to allow comparisons, particularly between sites.
Vertical Ionization Energies of α-L-Amino Acids as a Function of Their Conformation: an Ab Initio Study

Vertical ionization energies (IE) as a function of the conformation are determined at the quantum chemistry level for eighteen α-L-amino acids. Geometry optimization of the neutrals are performed within the Density Functional Theory (DFT) framework using the hybrid method B3LYP and the 6-31G**(5d) basis set. Few comparisons are made with wave-function-based ab initio correlated methods like MP2, QCISD or CCSD. For each amino acid, several conformations are considered that lie in the range 10-15 kJ/mol by reference to the more stable one. Their IE are calculated using the Outer-Valence-Green's-Functions (OVGF) method at the neutrals' geometry. Few comparisons are made with MP2 and QCISD IE. It turns out that the OVGF results are satisfactory but an uncertainty relative to the most stable conformer at the B3LYP level persists. Moreover, the value of the IE can largely depend on the conformation due to the fact that the ionized molecular orbitals (MO) can change a lot as a function of the nuclear structure.
Enhancement of β-Mannanase Production by Bacillus subtilis ATCC11774 through Optimization of Medium Composition

Palm kernel cake (PKC) has been largely produced in Malaysia as one of the cheap and abundant agro-waste by-products from the palm oil industry and it contains high fiber (mannan) content. The present study aimed to produce β-mannanase by Bacillus subtilis ATCC11774 via optimization of the medium composition using palm kernel cake as substrate in semi-solid fermentation. The fermentation nutrients such as PKC, peptone, yeast extract, sodium chloride, magnesium sulphate (MgSO2), initial culture pH and temperature were screened using a Plackett-Burman design. The three most significant factors identified, PKC, peptone and NaCl, were further optimized using central composite design (CCD), a response surface methodology (RSM) approach, where yeast extract and MgSO2 were fixed as a constant factor. The maximum β-mannanase activity predicted by CCD under the optimum medium composition of 16.50 g/L PKC, 19.59 g/L peptone, 3.00 g/L yeast extract, 2.72 g/L NaCl and 0.2 g/L MgSO2 was 799 U/mL. The validated β-mannanase activity was 805.12 U/mL, which was close to the predicted β-mannanas activity. As a comparison, commercial media such as nutrient broth, M9 and Luria bertani were used for the production of β-mannanase with activities achieved at 204.16 ± 9.21 U/mL, 50.32 U/mL and 88.90 U/mL, respectively. The optimized PKC fermentation medium was four times higher than nutrient broth. Hence, it could be a potential fermentation substrate for the production of β-mannanase activity by Bacillus subtilis ATCC11774.
SINAT E3 Ubiquitin Ligases Mediate FREE1 and VPS23A Degradation to Modulate Abscisic Acid Signaling

The SINAT E3 ubiquitin ligases regulate ABA signaling by modulating the protein stability of ESCRT components FREE1 and VPS23A. In plants, the ubiquitin-proteasome system, endosomal sorting, and autophagy are essential for protein degradation; however, their interplay remains poorly understood. Here, we show that four Arabidopsis (Arabidopsis thaliana) E3 ubiquitin ligases, SEVEN IN ABSENTIA OF ARABIDOPSIS THALIANA1 (SINAT1), SINAT2, SINAT3, and SINAT4, regulate the stabilities of FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING1 (FREE1) and VACUOLAR PROTEIN SORTING23A (VPS23A), key components of the endosomal sorting complex required for transport-I, to modulate abscisic acid (ABA) signaling. GFP-SINAT1, GFP-SINAT2, and GFP-SINAT4 primarily localized to the endosomal and autophagic vesicles. SINATs controlled FREE1 and VPS23A ubiquitination and proteasomal degradation. SINAT overexpressors showed increased ABA sensitivity, ABA-responsive gene expression, and PYRABACTIN RESISTANCE1-LIKE4 protein levels. Furthermore, the SINAT-FREE1/VPS23A proteins were codegraded by the vacuolar pathway. In particular, during recovery post-ABA exposure, SINATs formed homo- and hetero-oligomers in vivo, which were disrupted by the autophagy machinery. Taken together, our findings reveal a novel mechanism by which the proteasomal and vacuolar turnover systems regulate ABA signaling in plants.
A Geometrical Perspective on Image Style Transfer With Adversarial Learning

Recent years witness the booming trend of applying generative adversarial nets (GAN) and its variants to image style transfer. Although many reported results strongly demonstrate the power of GAN on this task, there is still little known about neither the interpretations of several fundamental phenomenons of image style transfer by generative adversarial learning, nor its underlying mechanism. To bridge this gap, this paper presents a general framework for analyzing style transfer with adversarial learning through the lens of differential geometry. To demonstrate the utility of our proposed framework, we provide an in-depth analysis of Isola et al.’s pioneering style transfer model pix2pix [1] and reach a comprehensive interpretation on their major experimental phenomena. Furthermore, we extend the notion of generalization to conditional GAN and derive a condition to control the generalization capability of the pix2pix model. From a higher viewpoint, we further prove a learning-free condition to guarantee the existence of infinitely many perfect style transfer mappings. Besides, we also provide a number of practical suggestions on model design and dataset construction based on these derived theoretical results to facilitate further researches.
Children's Books as Cultural Products: A Qualitative Study of Cultural Representation in Hmong and Non-Hmong American Books

CHILDREN’S BOOKS AS CULTURAL PRODUCTS: A QUALITATIVE STUDY OF CULTRUAL REPRESENTATION IN HMONG AND NON-HMONG AMERICAN BOOKS by Malina Her This study examined the type of cultural practices and values depicted within Hmong American children’s books in comparison to non-Hmong American children’s books from the United States. The purpose was to explore if prior Hmong traditional practices and values reflective of American individualism would extend to Hmong children’s books. Thirty best-seller children’s books were coded using two checklists, one focused on Hmong traditional practices and the other on American values. Results showed that Hmong traditional practices underscored by Hmong adolescents in prior research somewhat extended to Hmong children’s books. Moreover, in some respects Hmong children’s books displayed similar numbers of American values as did American children’s books. This study expanded the ethnic-racial socialization literature to an understudied population, the Hmong. In addition, the study provides parents and public educators insights into the cultural practices and values presented within Hmong children’s books.
Effects of Maresin 1 (MaR1) on Colonic Inflammation and Gut Dysbiosis in Diet-Induced Obese Mice

The aim of this study was to characterize the effects of Maresin 1 (MaR1), a DHA-derived pro-resolving lipid mediator, on obesity-related colonic inflammation and gut dysbiosis in diet-induced obese (DIO) mice. In colonic mucosa of DIO mice, the MaR1 treatment decreased the expression of inflammatory genes, such as Tnf-α and Il-1β. As expected, the DIO mice exhibited significant changes in gut microbiota composition at the phylum, genus, and species levels, with a trend to a higher Firmicutes/Bacteroidetes ratio. Deferribacteres and Synergistetes also increased in the DIO animals. In contrast, these animals exhibited a significant decrease in the content of Cyanobacteria and Actinobacteria. Treatment with MaR1 was not able to reverse the dysbiosis caused by obesity on the most abundant phyla. However, the MaR1 treatment increased the content of P. xylanivorans, which have been considered to be a promising probiotic with healthy effects on gut inflammation. Finally, a positive association was found between the Deferribacteres and Il-1β expression, suggesting that the increase in Deferribacteres observed in obesity could contribute to the overexpression of inflammatory cytokines in the colonic mucosa. In conclusion, MaR1 administration ameliorates the inflammatory state in the colonic mucosa and partially compensates changes on gut microbiota caused by obesity.
A Numerical Investigation of Enhancing Microfluidic Heterogeneous Immunoassay on Bipolar Electrodes Driven by Induced-Charge Electroosmosis in Rotating Electric Fields

A unique approach is proposed to boost on-chip immuno-sensors, for instance, immunoassays, wherein an antibody immobilized on the walls of a microfluidic channel binds specifically to an antigen suspended freely within a working fluid. The performance of these sensors can be limited in both susceptibility and response speed by the slow diffusive mass transfer of the analyte to the binding surface. Under appropriate conditions, the binding reaction of these heterogeneous immuno-assays may be enhanced by electroconvective stirring driven by external AC electric fields to accelerate the translating motion of antigens towards immobilized antibodies. To be specific, the phenomenon of induced-charge electroosmosis in a rotating electric field (ROT-ICEO) is fully utilized to stir analyte in the vicinity of the functionalized surface of an ideally polarizable floating electrode in all directions inside a tri-dimensional space. ROT-ICEO appears as a consequence of the action of a circularly-polarized traveling wave signal on its own induced rotary Debye screening charge within a bipolar induced double layer formed on the central floating electrode, and thereby the pertinent electrokinetic streamlines exhibit a radially converging pattern that greatly facilitates the convective transport of receptor towards the ligand. Numerical simulations indicate that ROT-ICEO can enhance the antigen–antibody binding reaction more effectively than convectional nonlinear electroosmosis driven by standing wave AC signals. The effectiveness of ROT-ICEO micro-stirring is strongly dependent on the Damkohler number as well as the Peclet number if the antigens are carried by a continuous base flow. Our results provide a promising way for achieving a highly efficient heterogeneous immunoassay in modern micro-total-analytical systems.
Developmental Speed Affects the Cryotolerance of In Vitro Produced Buffalo (Babalus Bubalis) Embryos

The aim of this study was to evaluate whether the developmental speed affects the cryotolerance of in vitro produced buffalo embryos. In Experiment 1, abattoir-derived oocytes were in vitro matured, fertilized and cultured. The embryos produced by Say 7 of culture were vitrified at the tight morula (TM), early blastocyst (EBL), blastocyst (BL), expanded-blastocyst (XBL) and hatched-blastocyst (HBL) stage. The embryos were vitrified by cryotop in 16.5% ethylene glycol (EG) and 16.5% dimethyl sulfoxide (DMSO) and 0.5 M sucrose. Embryos were warmed in 0.25 M sucrose for 1 min and then in 0.15 M sucrose for 5 min and cultured in vitro for 24 h, to evaluate post-culture viability. In Experiment 2, ovum pick-up (OPU) was carried out on lactating buffaloes to produce embryos that were vitrified-warmed and transferred into synchronized recipients. The lowest (P<0.01) survival rates were recorded with TM (22.4%) and the highest (P<0.01) with HBL (84.5%), whereas intermediate results were observed with EBL, BL and XBL (54.5, 64.7, 67.9%, respectively). Pregnancy rate on both Days 25 and 45 was significantly higher (P<0.05) for faster (XBL and HBL) developing embryos (75.0% and 62.5%, respectively) than for the slower (TM, EBL and BL) developing counterparts (36.4% and 0, respectively). Interestingly, pregnancies to term were only recorded when HBL were transferred (20%). In conclusion, it was demonstrated that the chronology of development is a major factor affecting the cryotolerance of in vitro produced buffalo embryos.
Effects of Replacing Extruded Maize by Dried Citrus Pulp in a Mixed Diet on Ruminal Fermentation, Methane Production, and Microbial Populations in Rusitec Fermenters

Simple Summary Citrus pulp is the main by-product obtained from citrus processing. The high-moisture content of this by-product makes it rapidly perishable, its accumulation can cause environmental problems, and it causes high disposal costs for citrus processing factories. Therefore, alternative uses for citrus pulp are necessary, its use in ruminant feeding being one of the most feasible ones. In this study, we assessed the effects of replacing extruded maize in a diet for dairy sheep (20% of diet) by dried citrus pulp using an in vitro technique (Rusitec fermenters). Results showed some positive effects of citrus pulp on diet degradability and in vitro fermentation parameters. The growth of ruminal microbes and bacterial diversity were essentially unaffected. Our results indicate that maize in dairy sheep diets can be totally replaced by dried citrus pulp without negatively affecting ruminal fermentation. The use of citrus pulp would reduce the amount of human-edible ingredients used in the diet of dairy sheep. Abstract Citrus pulp is a highly abundant by-product of the citrus industry. The aim of this study was to assess the effects of replacing extruded maize (EM; 20% of total diet) by dried citrus pulp (DCP; 20%) in a mixed diet on rumen fermentation and microbial populations in Rusitec fermenters. The two diets contained 50% alfalfa hay and 50% concentrate, and the same protein level. Four Rusitec fermenters were used in a cross-over design with two 13-d incubation runs. After 7-d of diet adaptation, diet disappearance, fermentation parameters, microbial growth, and microbial populations were assessed. Fermenters receiving the DCP showed greater pH values and fiber disappearance (p < 0.001) and lower methane production (p = 0.03) than those fed EM. Replacing EM by DCP caused an increase in the proportions of propionate and butyrate (p < 0.001) and a decrease in acetate (p = 0.04). Microbial growth, bacterial diversity, and the quantity of bacteria and protozoa DNA were not affected by the diet, but the relative abundances of fungi and archaea were greater (p < 0.03) in solid and liquid phases of DCP fermenters, respectively. Results indicate that DCP can substitute EM, promoting a more efficient ruminal fermentation.
Personalized prediction of daily eczema severity scores using a mechanistic machine learning model

Atopic dermatitis (AD) is a chronic inflammatory skin disease with periods of flares and remission. Designing personalized treatment strategies for AD is challenging, given the apparent unpredictability and large variation in AD symptoms and treatment responses within and across individuals. Better prediction of AD severity over time for individual patients could help to select optimum timing and type of treatment for improving disease control.
Risk and Protective Factors for the Onset of Cognitive Impairment in Korea: A 10-Year Longitudinal Panel Study

Objective This study aimed to examine the associations between multiple modifiable risk/protective factors and the onset of cognitive impairment, using nationally representative panel data spanning 10 years. Methods A total of 7,568 respondents who were cognitively normal at baseline were included and followed up for 10 years using data from the 2006–2016 Korean Longitudinal Study of Ageing. The data were converted into 28,113 person-year observations, and a life table approach and a series of event history models were used to estimate the transition rates to cognitive impairment over time and to examine the influence of time-varying factors on the onset of cognitive impairment. Cognitive impairment was measured using the Korean version of the Mini-Mental State Exam. Results About half of the respondents without cognitive impairment at baseline had experienced cognitive impairment by the end of the last interview, and women were more likely than men to experience cognitive impairment during the observation period. Vascular risk factors, hearing loss, socioeconomic status, regular exercise, participation in social activities, and frequent contact with close friends were significantly associated with the onset of cognitive impairment. Conclusion This investigation of modifiable factors for cognitive impairment using population-based data may lead to the identification of preventive strategies that people could integrate into their lifestyles.
Learning Representations for Facial Actions From Unlabeled Videos

Facial actions are usually encoded as anatomy-based action units (AUs), the labelling of which demands expertise and thus is time-consuming and expensive. To alleviate the labelling demand, we propose to leverage the large number of unlabelled videos by proposing a twin-cycle autoencoder (TAE) to learn discriminative representations for facial actions. TAE is inspired by the fact that facial actions are embedded in the pixel-wise displacements between two sequential face images (hereinafter, source and target) in the video. Therefore, learning the representations of facial actions can be achieved by learning the representations of the displacements. However, the displacements induced by facial actions are entangled with those induced by head motions. TAE is thus trained to disentangle the two kinds of movements by evaluating the quality of the synthesized images when either the facial actions or head pose is changed, aiming to reconstruct the target image. Experiments on AU detection show that TAE can achieve accuracy comparable to other existing AU detection methods including some supervised methods, thus validating the discriminant capacity of the representations learned by TAE. TAE’s ability in decoupling the action-induced and pose-induced movements is also validated by visualizing the generated images and analyzing the facial image retrieval results qualitatively and quantitatively.
Application of end-of-shift respirable crystalline silica monitoring to construction

Abstract A pilot project was conducted to determine the effect of common construction dusts as interferences in a new portable end-of-shift (EoS), direct-on-filter (DoF) sampling and analysis method for respirable crystalline silica (RCS), in this case, quartz. Construction dusts were prepared from plaster, drywall, cement and brick by grinding, aerosolizing, and collecting respirable dust with high flow rate cyclones. Filters were loaded with different levels of commercial α-quartz powder Min-u-Sil 5, and different levels of interfering dusts, singly and in combination. Samples were analyzed by Fourier Transform Infrared Spectroscopy (FTIR). Good correlations were found between nominal quartz loading (0 µg, 25 µg, 50 µg, and 100 µg) adjusted for quartz in the interfering dust and FTIR absorbance alone and in the presence of all interfering dusts. The slopes of the correlations were similar whether the loading was quartz without interference, or with plaster, drywall, and cement dusts, regardless of quantity. The results show that (a) plaster and drywall dusts do not interfere substantially; (b) cement does not interfere, but a change in the intercept suggests an effect on the background absorbance of the filter; and (c) in addition to having a substantial quartz content, brick dust contains an additional material, probably a silicate mineral, which interferes with the quartz peak. Thus, the presence of cement leads to lower quartz values and brick leads to higher values, but overall, 83% of the quartz contents predicted from the calibration data agreed within 50% of the adjusted nominal loadings within the range 20–110 µg. This result is encouraging given the high levels of interfering dusts. Nine samples loaded with smaller amounts of all four dusts together gave results within 25% of the adjusted nominal loadings. A single mixture addition of the dusts to the filter gave tighter variance in results than sequential additions. Unexpectedly, the two Certified Reference Materials (CRMs) 1878a and 1878b, gave different results when used to calibrate XRD analysis of Min-u-Sil 5.