Miracle-dz/UniFAR-FTUs huggingface dataset:
Reducing Allergenicity of Soy Protein Isolate from Several Varieties of Soybean through Glycation with Lactose

Food allergy is a specific immunological response caused by allergens contained in food. Soybean is one of the eight types of food products that most frequently cause allergies, which may lower its quality in terms of safety aspects. Soybean processing can reduce the risk of allergies by modifying the soy protein structure so as to produce hypoallergenic food. Processing involving Maillard reaction by conjugating proteins with reducing sugars has the potential to reduce allergenicity. This research aims to: (1) determine the degree of glycation based on the formation of soy protein isolates (SPI) -lactose conjugate and free amino; (2) determine the molecular weight profile of SPI and SPI-lactose conjugate: and (3) analyze the allergenicity of SPI and SPI-lactose conjugate. Protein isolation was carried out by protein precipitation of imported soybean (GMO and non-GMO) and local soybean varieties (Anjasmoro and Grobogan) at their isoelectric point or using a pH arrangement. Then the SPI was reacted with lactose under pH 9.5 and 95 ° C for 60 minutes. The determination of glycation degree of SPI-lactose conjugate was carried out using two methods, namely the thiobarbituric acid reactive substances (TBARS) method and Bradford method for free amino acids. The protein molecular weight profile was analyzed using the SDS-PAGE electrophoresis method. The allergenicity of SPI and SPI-lactose conjugate were analyzed quantitatively using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The analysis results Current Research in Nutrition and Food Science www.foodandnutritionjournal. org ISSN: 2347-467X, Vol. 08, No. (1) 2020, Pg. 268-280 CONTACT Nurheni Sri Palupi hnpalupi@yahoo.com Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Bogor, Indonesia. © 2020 The Author(s). Published by Enviro Research Publishers. This is an Open Access article licensed under a Creative Commons license: Attribution 4.0 International (CC-BY). Doi: http://dx.doi. org/10.12944/CRNFSJ.8. 1.25 Article History Received: 9 November 2019 Accepted: 24 March 2020
THE EFFECT OF SERVICE QUALITY, PERCEIVED VALUE AND MEDIATING EFFECT OF BRAND IMAGE ON BRAND TRUST

This study aimed to determine the effect of service quality on perceived value mediated by brand image and brand trust on the participant BPJS KESEHATAN Kesehatan Branch Malang (National Health Insurance Office Malang Branch). This study is using purposive sampling, 170 people who registered as social security administration's health Malang branch were selected as the respondents. All variables are measured by Likert scale. The Data was analyzed using partial least squares. The results are service quality influences significant ability to trust and brand image, and perceived value does not significantly influence trust, but significant influences brand image. Brand image partially mediates the effect of service quality on trust and fully mediates the effect of perceived value on trust. The implication of this research is social security administration health Malang branch must maintain and improve the quality of its services, promote and socialize to improve its brand image and brand trust.
Auditors’ conflict of interest: does random selection work?

Third-party auditors are usually chosen and paid by the agent that is being audited and this may lead to a significant conflict of interest and to less strict audits. We investigate the effect of a new random allocation mechanism, according to which, starting from 2012, auditors of Italian municipalities have had to be chosen by means of a random draw from a large pool of experts. By exploiting the staggered adoption of the new allocation rule across municipalities, our difference-in-differences estimates show that the new regime implies a worsening of municipalities’ reported public finances, in terms of budget surpluses of the probability to be in financial distress. The effect is largely driven from municipalities endowed with lower social capital, so signalling that random allocation is somehow a substitute for the solution of the conflict of interest problem. In these municipalities, we also find that the new mechanism reduces some fraud detection indicators based on Benford’s law.
The correlation between science process skills and biology cognitive learning outcome of senior high school students

Science Process Skills (SPS) are the essential abilities which need to be mastered in learning biology to strengthen students’ learning outcomes. The aim of this study was to analyze the relationship between SPS and biology Cognitive Learning Outcome (CLO) of senior high school students. The sample was 100 tenth graders in Malang. The correlational study used two test types as the instrument (i.e. essay and multiple choice). The essay instrument consisted of 10 items which were developed from SPS aspects (basic and integrated skills). Meanwhile, the biology CLO was measured using multiple choice test which consisted of 30 items. The collected data were analyzed using simple linear regression analysis. The results showed that the highest students’ SPS aspect was observing (78.84%), while the lowest was predicting aspect (27.30%). In addition, the highest achievement of students’ biology CLO was C1 (94.23%), while the lowest was C6 (33.46%). The regression analysis showed that there was a significant correlation between students’ SPS and biology CLO, where the equation was Y = 27.988 + 0.311X. Therefore, it is crucial to consider SPS to be integrated into a learning activity by using various learning models that empowering these skills to improve students’ biology CLO.
Influence of certain plant products on the insect pollinators of coriander.

Investigations were carried out to evaluate the “Influence of certain plant products on the insect pollinators of coriander” at Rajasthan College of Agriculture, Udaipur, during rabi 2008-09. The treatments comprised: Pongamia glabra Vent (seed oil – 1 %), Azadirachta indica A. Juss (seed kernel extract 5%), formulated neem product (Achook 0.8%), Azadirachta indica (seed oil 1%), Azadirachta indica (leaf extract 10%) replicated 4 times. Reduction in pollinator population was estimated one day after application of the botanicals; whereas, 3 and 5 days after the treatment the mean percentage increase or decrease in the pollinator abundance over control was computed. The plant origin insecticides evinced a decrease in pollinator intensity ranging from (-) 14.52 to (-) 25.06 per cent. The maximum reduction of insect pollinators (25.06%) was recorded from plots treated with neem seed oil (1%). Three days after the treatment the percentage of visiting insect pollinators did not change in plots treated with karanj oil (1%), but the pollinator percentage increased in plots treated with NSKE (5%) and neem leaf extract (10%). Most of the botanical treatments did not have any side-effect 5 days after the treatment; however, plots treated with seed oil (1%) of Azadirachta indica A. Juss continued to show a deterrent effect and the pollinator intensity decreased by 21.57 per cent.
The influence of catch crops on fungal diversity in the soil and health of oat

The aim of the research was to determine the quantitative and qualitative composition of soil-borne fungi in the cultivation of oat, as well as to assess the healthiness of this cereal. The experiment considered spring barley and potato as forecrops, and white mustard or lacy phacelia cultivated after spring barley as catch crops before oat. The population of fungi showing antagonistic effect towards selected fungi pathogenic to cereal was determined. The most fungi (pathogenic and antagonistic) were found in the soil under oat cultivation after spring barley, while the least after potato. The cultivation of oat after spring barley with the use of catch crops significantly influenced a reduction in the number of fungi obtained from the soil. On the other hand, catch crop cultivation increased the share of antagonists in the soil fungus population. Among the species recognised as antagonistic, Clonostachys rosea, Trichoderma viride, T. koningii, and T. harzianum predominated. The introduction of catch crops, especially white mustard, also positively influenced the healthiness of oat. The average disease index for the tested oat cultivars grown after potato and after spring barley with white mustard and lacy phacelia as catch crops was 11.02, 12.78 and 15.90, respectively, whereas after spring barley it was 21.75.
Identification of hemocyte populations in mud crab (Scylla olivacea) by microscopy and flow cytometry

The circulating hemocytes of crustaceans are essential in host immune response. The study will identify hemocyte population in mud crab ( Scylla olivacea ), based mainly on the presence of cytoplasmic granules. Three types of hemocyte of S. olivacea were identified under the light microscope (LM). Hyaline cell (HC) has no cytoplasmic granules. Small granular cell (SGC) has small cytoplasmic granules. Large granular cell (LGC) has large cytoplasmic granules with high density in cytoplasm. By flow cytometry and transmission electron microscopy (TEM) crab hemocytes were divided into HC, SGC, IGC and LGC. The intermediate granular cell (IGC) has intermediate size of cytoplasmic granules between those of HC and LGC
An orphaned clone of Potamogeton × schreberi in the Czech Republic.

A Potamogeton hybrid found growing in the absence of parental species in a South Bohemian stream, Czech Republic, was subjected to molecular analyses to identify its exact identity. RFLP of the ITS region confirmed its previous morphological identification as P. natans × P. nodosus (= P. ×schreberi). A comparison of its RFLP pattern with those of P. gramineus, P. lucens and P. polygonifolius unambiguously excluded the possibility that the investigated plants are specimens of other similar hybrids (P. ×fluitans, P. ×sparganiifolius, P. ×gessnacensis). The discovery of P. ×schreberi in South Bohemia is the first record of this hybrid for the Czech Republic. So far, it is known only from five countries and the Czech clone is one of a few extant clones of this hybrid in Central Europe. Chloroplast DNA sequencing identified P. nodosus as the maternal parent although at present this species neither occurs at the locality, nor upstream, nor in the entire drainage basin. The other species, P. natans, only occurs downstream of the locality in isolated side pools in a former stream bed and fishponds in an adjacent drainage basin. The available data indicate that this hybrid has persisted vegetatively at this locality for some time in the absence of its parents.
Monocyte Chemoattractant Protein-1 and CCR2 Interactions Are Required for IFN-α/β-Induced Inflammatory Responses and Antiviral Defense in Liver1

IFN-α/β-mediated functions promote production of MIP-1α (or CCL3) by mediating the recruitment of MIP-1α-producing macrophages to the liver during early infection with murine CMV. These responses are essential for induction of NK cell inflammation and IFN-γ delivery to support effective control of local infection. Nevertheless, it remains to be established if additional chemokine functions are regulated by IFN-α/β and/or play intermediary roles in supporting macrophage trafficking. The chemokine MCP-1 (or CCL2) plays a distinctive role in the recruitment of macrophages by predominantly stimulating the CCR2 chemokine receptor. Here, we examine the roles of MCP-1 and CCR2 during murine CMV infection in liver. MCP-1 production preceded that of MIP-1α during infection and was dependent on IFN-α/β effects for induction. Resident F4/80+ liver leukocytes were identified as primary IFN-α/β responders and major producers of MCP-1. Moreover, MCP-1 deficiency was associated with a dramatic reduction in the accumulation of macrophages and NK cells, as well as decreased production of MIP-1α and IFN-γ in liver. These responses were also markedly impaired in mice with a targeted disruption of CCR2. Furthermore, MCP-1- and CCR2-deficient mice exhibited increased viral titers and elevated expression of the liver enzyme alanine aminotransferase in serum. These mice also had widespread virus-induced liver pathology and succumbed to infection. Collectively, these results establish MCP-1 and CCR2 interactions as factors promoting early liver inflammatory responses and define a mechanism for innate cytokines in regulation of chemokine functions critical for effective localized antiviral defenses.
The Elasticity of the tonoplast membrane related to its ultrastructure and chemical composition

The osmotic behavior of yeast (Saccharomyces cerevisiae) vacuoles was investigated both in vivo and in vitro. Freeze-etch studies revealed an overall increase in the number of fracture face particles after swelling and an overall decrease after shrinking. In addition changes of particle diameter occured on both fracture faces. The tonoplast was chemically analysed, as were the yeast plasmalemma and erythrocyte membrane for comparison. Differences in the chemical compo¬ sition of these membranes,and the freeze-etching results, were interpreted to develop a membrane concept which allows for the grater elasticity of the tonoplast as compared with plasmalemma and erythrocyte membrane.
Existence of a unique positive entropy solution to a singular fractional Laplacian

In this paper, we study the existence of a positive solution to the elliptic problem: Here (N>2s) is an open bounded domain with smooth boundary, and . For , we take advantage of the convexity of Ω. The operator indicates the restricted fractional Laplacian, and μ is a non-negative Radon measure as a source term. The assumptions on f and h will be precised later. Besides, we will discuss the notion of entropy solution and its uniqueness for some specific measures.
Shear Performance for Prestressed Concrete Hollow Core Slabs

This study evaluated the shear performance of prestressed concrete hollow core slabs (HCS), which are convenient to use as floor structures of flexible spaces. A total of 18 specimens, with cross-sectional height and presence of topping concrete as variables, were fabricated by extrusion. A four-point loading test was conducted using simply supported beams. The results showed that shear performance satisfied the requirements of ACI 318-19 regardless of cross-sectional height or presence of topping concrete. Through comparison with past studies, the web-shear strength of HCS was found to be influenced by compressive stress due to prestress at the centroid, compressive strength of concrete, and shear span-to-depth ratio.
‘Swamped’: the populist construction of fear, crisis and dangerous others in Pauline Hanson’s senate speeches

ABSTRACT This paper critically examines the discursive construction of fear, crises, threat and dangerous Others in Pauline Hanson’s populist political communication from 2016–2018. Specifically, it looks at how a politics of fear manifests through the construction of Islam and Muslims as Others who pose an existential threat to Australia’s security and ‘culture’. Drawing on critical discourse analysis, this paper examines the way in which discursive strategies and other linguistic devices are used purposefully in order to serve Hanson’s right-wing populist agenda. This paper argues that Hanson is emblematic of both liberal and illiberal Islamophobia and that she strategically draws on liberal critiques of Islam to justify draconian policies such as immigration bans and restrictions on religious freedoms. Underpinning this research is the belief that populist communication is consequential and must be examined for a holistic understanding of the populist phenomenon.
Interactive game “Kahoot!” as the media of students’ vocabulary assessment

This research aims to investigate students’ perspectives on using the interactive game “Kahoot! ” as the media of vocabulary test at one of the public senior high schools in Yogyakarta Indonesia. It was qualitative research with five voluntarily participating students as the participants. An in-depth semi-structured interview was applied as the data collection technique. The theme-based analysis was used in this study. The finding shows that first, Kahoot! is more practical for a vocabulary test. Second, the game can attract the students’ attention to the test. Third, it helps the students to be more active in the classroom and the last, the game makes the students more enthusiastic in doing the test. However, this research also found some negative sides of using Kahoot! ; first, the students become less confident with their score, especially when they get lower scores than other students because the scores were displayed on the leader board in Kahoot! application; second, some students had a lack of knowledge in using the application; and the last, the time was limited. This study implies that the use of Kahoot! in the EFL classroom can be an alternative for teachers to teach vocabulary and provide fun learning activities for the students.
Proteomic and probit analyses of glufosinate-ammonium-resistant goosegrass(Eleusine indica (L.) Gaertn.) biotypes in Malaysia / Adam bin Jalaludin

Goosegrass (Eleusine indica [L.] Gaertn.), regarded as one of the world’s worst weeds is highly pernicious to cash crop growers in Malaysia. Following reports in 2009 that glufosinate ammonium failed to adequately control goosegrass populations in Kesang, Malacca and Jerantut, Pahang, Malaysia, on-site field trials were conducted to assess the efficacy of glufosinate-ammonium and glyphosate towards goosegrass from both places. Glufosinate-ammonium at 495 g ai ha1 managed to provide 82% control of the weed at the vegetable farm while the same rate failed to control goosegrass at the oil palm nursery. Glyphosate failed in controlling goosegrass population at both places where the highest rate (4320 g ae ha-1) produced 13% and 3% control, respectively. The efficacy of both herbicides was also tested on the Kesang and Jerantut goosegrass grown from seeds. Glufosinate-ammonium at the recommended rate provided satisfactory control of the Kesang biotype while the same rate failed to control Jerantut biotype. Glyphosate at 540 g ae ha-1 again failed in damaging both biotypes. The highest rate used managed to control the Kesang biotype but still did not effectively damage the Jerantut biotype. Comparison with susceptible goosegrass showed that the ‘Kesang’ biotype was 1 and 6-fold more resistant to glyphosate and glufosinateammonium respectively while the ‘Jerantut’ biotype was 3- and 30-fold more resistant to glyphosate and glufosinate-ammonium respectively. The low glyphosate resistance index (R.I) value for both biotypes were believed to be caused by the significant tolerance of the susceptible biotype against glyphosate. Proteomic analysis was conducted to see any differences in the proteins expressed by the susceptible, the Kesang and the Jerantut biotypes. There were 150 matched spots between the susceptible and the Jerantut biotypes, with 4 spots differentially expressed. Between the susceptible and the Kesang biotypes, a total of 145 spots were matched, but only 3 spots were differentially expressed. Most of the differences in abundance were due to the presence or absence of a protein in either the susceptible or the Jerantut and Kesang biotypes. MALDI-TOF analysis successfully identified the identities of ten spots from the Jerantut biotype proteome. They include peptidyl-prolyl cis-trans isomerase, ferredoxin NADP+ reductase, peroxiredoxin, granule bound starch synthase, WD-repeat protein and a small subunit of RuBisCO. The remaining four proteins were unknown and hypothetical proteins. The functions of these protein ranges from folding of proteins, electron transfer, storage, DNA and RNA related processes, antioxidants and even stress-related functions. The occurrence of glufosinate-ammonium resistance in goosegrass calls for more research to better understand the resistance mechanism of this particular weed and more integrated management of the weed to prevent escalating resistance and further proliferation in the country.
Long‐term water retention increases in degraded soils amended with cross‐linked polyacrylamide

Correspondence USDA-ARS Northwest Irrigation and Soils Research Laboratory, 3793 N 3600 E, Kimberly, ID 83341, USA. Email: rick.lentz@usda.gov Abstract Cross-linked polymer hydrogels, polyacrylamide co-polymer (XPAM), and Kpolyacrylate (XPAA) increase soil water availability under drought, but their longterm effects are unknown despite the importance of such knowledge in assessing economic feasibility. This 9-yr outdoor pot study evaluated a one-time addition of XPAM or XPAA at 0.25 or 0.5% dry weight (5.6 or 11.2 Mg ha−1 ) in a degraded calcareous silt loam. Controls included an unamended degraded soil and an unamended nondegraded soil (topsoil). Soil water retention and plant available water (PAW, g water g−1 dry soil−1 ) were measured in soil samples collected in spring for seven of the nine years. Across all years, the 0.5% XPAM produced the greatest PAW (0.318) and the PAW of other treatments followed in the order: 0.5% XPAM > 0.25% XPAM > Topsoil > 0.5% XPAA 0.25% XPAA > = control (0.224). In all years, the 0.25% XPAM and 0.5% XPAM treatments increased soil PAW relative to the control, that is, their PAW ratios exceeded unity. Topsoil PAW exceeded that of the control in six of the seven years measured. The PAW of 0.25% XPAM and 0.5% XPAM peaked in Year 1 after application and declined linearly with time (P < .03), at −0.0036 yr −1 and −0.0044 yr −1 , respectively. Hence, the mean residence time the XPAM-related waterretention benefit is 24–29 yr. In this study, soil water-retention benefits from XPAM amendments exceeded projections proposed by the industry (5 yr) and suggests that the cost-benefits of field-level XPAM applications might be more favorable than previously anticipated.
Genetic Diversity Analysis for Quantitative Traits in Advanced Breeding Lines of Sesame (Sesamum indicum L.)

The word sesame come from Latin word 'sesamum' and Greek word 'sesamon' which means seed or fruit of sesame plant. Sesame (Sesamum indicum. L), also known as 'ellu' in 'Kannada’, ‘til' in Hindi and 'nuvvulu' in Telugu, it is most ancient crop known to mankind. It is an indigenous crop of India after groundnut and brassica oilseeds. Weiss (1883) and several other were having view that it was originated in south western Africa. In Africa most of the cultivated and wild species of sesame occurs. It might have International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 12 (2019) Journal homepage: http://www.ijcmas. com
Estimation of Carbon Stock Monetary Value of a Tropical Rainforest in Nigeria

The study estimated the carbon stock contents of a lowland rainforest in Nigeria, Okomu National Park. Four major Carbon pools were estimated, they were: above-ground biomass; below-ground biomass: dead wood; and litter. Nested plot design was used for sample plot demarcation. All trees (living and dead) with Dbh ≥ 10cm were enumerated within the 50 m X 50 m and 20 m X 20 m plots, while the 1 m X 1 m quadrant was for litter samples collection. Core samples and sub-samples were collected from live tree and dead wood respectively. The total carbon stock estimated for the study area was 177.58 tons/ha, of which above-ground biomass accounted for 134.01 tons/ha and below-ground biomass was 33.50 tons/ha. Dead wood was 6.05 tons/ha while litter was 4.02 tons/ha. The Carbondioxide equivalent was estimated to be 651.14 tons/ha. The monetary value for the carbon stock estimated at $ 4/tCO2 was $ 52,674,679. Thus, carbon sequestration is one of the significant ecosystem services provided by mature rainforests. Keywords : Carbon pool, Rainforest, Ecosystem Service, Climate Change, Forest Management DOI: 10.7176/JRDM/63-06 Publication date: March 31 st 2020