Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence of sex and fiber type on the satellite cell pool in human skeletal muscle

The repair, remodeling, and regeneration of myofibers are dependent on satellite cells (SCs), although, the distribution of SCs in different fiber types of human muscle remains inconclusive. There is also a paucity of research comparing muscle fiber characteristics in a sex‐specific manner. Therefore, the aim of this study was to investigate fiber type‐specific SC content in men and women. Muscle biopsies from vastus lateralis were collected from 64 young (mean age 27 ± 5), moderately trained men (n = 34) and women (n = 30). SCs were identified by Pax7‐staining together with immunofluorescent analyses of fiber type composition, fiber size, and myonuclei content. In a mixed population, comparable number of SCs was associated to type I and type II fibers (0.07 ± 0.02 vs 0.07 ± 0.02 SCs per fiber, respectively). However, unlike men, women displayed a fiber type‐specific distribution, with SC content being lower in type II than type I fibers (P = .041). Sex‐based differences were found specifically for type II fibers, where women displayed lower SC content compared to men (P < .001). In addition, positive correlations (r‐values between 0.36‐0.56) were found between SC content and type I and type II fiber size in men (P = .03 and P < .01, respectively), whereas similar relationships could not be detected in women. Sex‐based differences were also noted for fiber type composition and fiber size, but not for myonuclei content. We hereby provide evidence for sex‐based differences present at the myocellular level, which may have important implications when studying exercise‐ and training‐induced myogenic responses in skeletal muscle.
Thalidomide in the treatment of erythema nodosum leprosum (ENL) in an outpatient setting: A five-year retrospective analysis from a leprosy referral centre in India

Erythema nodosum leprosum (ENL), or type 2 lepra reaction, is a multi-system immune-mediated complication in patients with multibacillary leprosy, frequently associated with chronicity and recurrences. Management of ENL requires high doses of oral corticosteroids, which may not be universally effective and pose serious adverse effects. Thalidomide has proven to be a steroid-sparing agent and is useful in controlling the reactions. However, many centres do not employ it in outpatient settings due to adverse effects and teratogenicity risk. Hence, we studied the feasibility of treating ENLs and reported the therapeutic outcome.This is a five-year record-based analysis of ENL leprosy patients treated with thalidomide, includingdescriptive statistics of demographic variables. Clinical characteristics were stratified by treatment compliance status (yes/no). Incidence rates and rate ratios for recovery stratified by bacillary index, type of ENL presentation and MDT treatment status were calculated.Out of 102 ENL patients treated with thalidomide, 68 (66.7%) were compliant and improved. Among them, ENL recurrence was noted in 11(16.2%) patients. The commonest thalidomide side effect was pedal oedema (73.5%). Patients with bacillary index (BI) less than or equal to 4.0 had a 37% increase in the incidence of recovery. Patients with acute ENL were almost twice as likely to recover as those with chronic ENL. Also, the improvement was two and a half times greater among those who completed MDT as compared to those on MDT. The study showed that thalidomide treatment for patients with ENL is possible in outpatientclinics. We also successfully prevented pregnancies to a larger extent through counselling for contraception.We observed that early institution of thalidomide induces faster remission and prevents ENL recurrence.
METHODS OF MEASURING BLOOD MEAL SIZE AND PROTEINASE ACTIVITY FOR DETERMINING EFFECTS OF MATED STATE ON DIGESTIVE PROCESSES OF FEMALE AEDES AEGYPTI (L.) (DIPTERA: CULICIDAE)

Abstract Two techniques for measuring size of ingested blood meal and 5 methods of measuring proteinase activity in the midgut of Aedes aegypti are compared. Protein, measured after trichloroacetic acid precipitation, was found to be most suitable for determining blood meal size and loss of protein from the gut. Of 5 proteinase substrates tested (BAPNA (benzoyl-DL-arginine-p-nitroanilide), hemoglobin, casein, azocasein, and hide powder azure), BAPNA was considered most suitable for trypsin measurements. These techniques were used to show that virgin females, 6 and 10 days after eclosion, consumed smaller meals than mated females. Virgin females also showed delays in both onset of protein loss from the gut and the increase in proteinase activity after blood feeding. Résumé On a comparé 2 techniques pour déterminer la quantité de sang ingérée et 5 méthodes pour mesurer l’activité d’une protéinase dans l’intestin moyen de Aedes aegypti. Après précipitation avec l’acide trichloroacétique, les protéines furent jugées appropriées pour évaluer la quantité de sang ingérée de même que pour mesurer la perte de protéines à partir de l’intestin. Des 5 substrats de protéinase testés (le BAPNA (benzoyl-DL-arginine-p-nitroaniline), l’hémoglobine, la caséine, l’azocaséine et le “hide powder azure”), le BAPNA fut considéré comme le plus approprié pour mesurer la trypsine. Ces techniques furent utilisées pour démontrer que les femelles vierges, 6 et 10 jours après leur émergence consomment moins que les femelles accouplées. Les femelles vierges commencent plus tard à perdre des protéines de l’intestin de même qu’à montrer une augmentation de l’activité de la protéinase après un repas.
Improving Speech Emotion Recognition With Adversarial Data Augmentation Network

When training data are scarce, it is challenging to train a deep neural network without causing the overfitting problem. For overcoming this challenge, this article proposes a new data augmentation network—namely adversarial data augmentation network (ADAN)— based on generative adversarial networks (GANs). The ADAN consists of a GAN, an autoencoder, and an auxiliary classifier. These networks are trained adversarially to synthesize class-dependent feature vectors in both the latent space and the original feature space, which can be augmented to the real training data for training classifiers. Instead of using the conventional cross-entropy loss for adversarial training, the Wasserstein divergence is used in an attempt to produce high-quality synthetic samples. The proposed networks were applied to speech emotion recognition using EmoDB and IEMOCAP as the evaluation data sets. It was found that by forcing the synthetic latent vectors and the real latent vectors to share a common representation, the gradient vanishing problem can be largely alleviated. Also, results show that the augmented data generated by the proposed networks are rich in emotion information. Thus, the resulting emotion classifiers are competitive with state-of-the-art speech emotion recognition systems.
Investigation of physicochemical, nutritional, and sensory qualities of muffins incorporated with dried brewer's spent grain flours as a source of dietary fiber and protein.

Brewers' spent grain (BSG) is the major byproduct of brewing beer, rich in protein and dietary fiber. This study investigated the effect of two drying methods (impingement and hot-air drying) on chemical composition, physicochemical properties, and bioactive compounds of BSGs from three different brewers (BSG1, BSG2, and BSG3), and then evaluated the quality and consumer acceptance of BSG flour fortified muffins. Results showed that impingement drying led to significantly lower moisture content (MC, 1.33-1.87 g/100g) and water activity (aw , 0.04-0.07) of BSGs than hot-air drying (5.44 to 5.57 g/100 g and 0.19 to 0.20, respectively). Among different dried BSGs, impingement dried BSG3 achieved the highest protein (18.03 g/100 g dry matter [DM]), total phenolic content (TPC, 2.21 mg GAE/g DM), radical scavenging activity (RSA, 1.58 mg AAE/g DM), and total flavonoid content (TFC, 0.68 mg QE/g DM), and retained lighter color (L*, 54.68) and higher total dietary fiber (TDF, 42.40 g/100 g DM), which was selected for making BSG-fortified muffins. BSG3 was substituted 1:1 as white: whole wheat flour at three concentrations (10, 15, and 20 g/100 g flour mix) for muffins (BSG10, BSG15, and BSG20, respectively). BSG15 provided higher protein (13.11 g/100 g DM), TDF (16.88 g/100 g DM), and higher bioactive compounds compared to control and retained brighter color of muffin compared to BSG20, showing no difference in firmness and overall liking compared to the control muffin. This study demonstrated that impingement dried BSG could be utilized as a functional ingredient in muffins to add value to the food chain providing nutritional and environmental benefits. PRACTICAL APPLICATION: This study reported the benefit of impingement drying method for the retention of physicochemical quality and bioactive compounds of brewer's spent grains (BSG) produced from three different brewers in comparison with hot-air drying. The study also reported that muffins fortified with BSG flours (15% replacement of wheat flour) yielded a 23% increase in total dietary fiber and 13% increase in protein without affecting consumer acceptance of the products. This information is essential for developing value-added applications of BSG, a byproduct from brewing industry, as a functional ingredient to make nutritive baking goods, such as muffins, for promoting human health.
Olfactory ensheathing cell mediated mechanisms of neurite outgrowth and axon regeneration

ii TABLE OF CONTENTS . iv LIST OF FIGURES . xi SYMBOLS, ABBREVIATIONS, AND NOMENCLATURE . xiv ACKNOWLEDGMENTS . xvii DEDICATION. xviii CHAPTER 1: LITERATURE REVIEW AND INTRODUCTION . 1 1.
The Impact of Protein Supplementation on Appetite and Energy Intake in Healthy Older Adults: A Systematic Review with Meta-Analysis.

Protein supplementation is an attractive strategy to prevent loss of muscle mass in older adults. However, it could be counterproductive due to adverse effects on appetite. This systematic review and meta-analysis aimed to determine the effects of protein supplementation on appetite and/or energy intake (EI) in healthy older adults. MEDLINE, The Cochrane Library, CINAHL, and Web of Science were searched up to June 2020. Acute and longitudinal studies in healthy adults ≥60 y of age that reported effects of protein supplementation (through supplements or whole foods) compared with control and/or preintervention (for longitudinal studies) on appetite ratings, appetite-related peptides, and/or EI were included. Random-effects model meta-analysis was performed on EI, with other outcomes qualitatively reviewed. Twenty-two studies (9 acute, 13 longitudinal) were included, involving 857 participants (331 males, 526 females). In acute studies (n = 8), appetite ratings were suppressed in 7 out of 24 protein arms. For acute studies reporting EI (n = 7, n = 22 protein arms), test meal EI was reduced following protein preload compared with control [mean difference (MD): -164 kJ; 95% CI: -299, -29 kJ; P = 0.02]. However, when energy content of the supplement was accounted for, total EI was greater with protein compared with control (MD: 649 kJ; 95% CI: 438, 861 kJ; P < 0.00001). Longitudinal studies (n = 12 protein arms) showed a higher protein intake (MD: 0.29 g ⋅ kg-1 ⋅ d-1; 95% CI: 0.14, 0.45 g ⋅ kg-1 ⋅ d-1; P < 0.001) and no difference in daily EI between protein and control groups at the end of trials (MD: -54 kJ/d; 95% CI: -300, 193 kJ/d; P = 0.67). While appetite ratings may be suppressed with acute protein supplementation, there is either a positive effect or no effect on total EI in acute and longitudinal studies, respectively. Therefore, protein supplementation may represent an effective solution to increase protein intakes in healthy older adults without compromising EI through appetite suppression. This trial was registered at PROSPERO as https://www.crd. york.ac. uk/prospero/display_record. php? ID=CRD42019125771 (CRD42019125771).
Recent Advances in Surface Activation of Polytetrafluoroethylene (PTFE) by Gaseous Plasma Treatments

Fluorinated polymers are renowned for their chemical inertness and thus poor wettability and adhesion of various coatings. Apart from chemical methods employing somewhat toxic primers, gaseous plasma treatment is a popular method for the modification of surface properties. Different authors have used different plasmas, and the resultant surface finish spans between super-hydrophobic and super-hydrophilic character. Some authors also reported the hydrophobic recovery. The review of recent papers is presented and discussed. Correlations between plasma and/or discharge parameters and the surface finish are drawn and the most important conclusions are summarized. The concentration of oxygen in the surface film as probed by X-ray photoelectron spectroscopy is inversely dependent on the concentration of oxygen in gaseous plasma. The predominant mechanism leading to hydrophilic surface finish is bond scission by deep ultraviolet radiation rather than functionalization with reactive oxygen species.
Cancer stemness of CD10-positive cells regulated by Hedgehog pathway promotes the resistance to cisplatin in oral squamous cell carcinoma.

OBJECTIVE To explore the role of CD10 in cisplatin-resistance of oral squamous cell carcinoma (OSCC) and its association with the Hedgehog (Hh) signaling pathway and cancer stem cells (CSCs). METHODS The correlation between cell viability and CD10 expression was analyzed in different OSCC cell lines after the cisplatin treatment. Genes related to chemotherapy-resistance, cancer stem cells, and the epithelial-mesenchymal transition were detected by qPCR in CD10high and CD10low OSCC cells. Mouse xenograft model and venous metastasis model were used to explore the potential regulatory mechanism of the resistance effect of CD10 on cisplatin. RESULTS The higher expression of CD10 gene in different cell lines displayed enhanced cisplatin-resistance ability. The expression of genes related to chemotherapy-resistance, cell stemness, and the epithelial-mesenchymal transition was significantly higher in CD10high cells compared with CD10low cells. Moreover, the combination of cisplatin and Hh pathway inhibitors significantly reduced the resistance of CD10 to cisplatin in the xenograft model and venous metastasis models. CONCLUSION CD10 positive cells are implicated in developing cisplatin-resistance of OSCC, which could be related to its cancer stem cell characteristics regulated by the Hedgehog pathway.
Trends in prevalence of acute stroke impairments: A population-based cohort study using the South London Stroke Register

Background Acute stroke impairments often result in poor long-term outcome for stroke survivors. The aim of this study was to estimate the trends over time in the prevalence of these acute stroke impairments. Methods and findings All first-ever stroke patients recorded in the South London Stroke Register (SLSR) between 2001 and 2018 were included in this cohort study. Multivariable Poisson regression models with robust error variance were used to estimate the adjusted prevalence of 8 acute impairments, across six 3-year time cohorts. Prevalence ratios comparing impairments over time were also calculated, stratified by age, sex, ethnicity, and aetiological classification (Trial of Org 10172 in Acute Stroke Treatment [TOAST]). A total of 4,683 patients had a stroke between 2001 and 2018. Mean age was 68.9 years, 48% were female, and 64% were White. After adjustment for demographic factors, pre-stroke risk factors, and stroke subtype, the prevalence of 3 out of the 8 acute impairments declined during the 18-year period, including limb motor deficit (from 77% [95% CI 74%–81%] to 62% [56%–68%], p < 0.001), dysphagia (37% [33%–41%] to 15% [12%–20%], p < 0.001), and urinary incontinence (43% [39%–47%) to 29% [24%–35%], p < 0.001). Declines in limb impairment over time were 2 times greater in men than women (prevalence ratio 0.73 [95% CI 0.64–0.84] and 0.87 [95% CI 0.77–0.98], respectively). Declines also tended to be greater in younger patients. Stratified by TOAST classification, the prevalence of all impairments was high for large artery atherosclerosis (LAA), cardioembolism (CE), and stroke of undetermined aetiology. Conversely, small vessel occlusions (SVOs) had low levels of all impairments except for limb motor impairment and dysarthria. While we have assessed 8 key acute stroke impairments, this study is limited by a focus on physical impairments, although cognitive impairments are equally important to understand. In addition, this is an inner-city cohort, which has unique characteristics compared to other populations. Conclusions In this study, we found that stroke patients in the SLSR had a complexity of acute impairments, of which limb motor deficit, dysphagia, and incontinence have declined between 2001 and 2018. These reductions have not been uniform across all patient groups, with women and the older population, in particular, seeing fewer reductions.
Arylsulfatase A (ASA) in Parkinson’s Disease: From Pathogenesis to Biomarker Potential

Parkinson’s disease (PD), the second most common neurodegenerative disorder after Alzheimer’s disease, is a clinically heterogeneous disorder, with obscure etiology and no disease-modifying therapy to date. Currently, there is no available biomarker for PD endophenotypes or disease progression. Accumulating evidence suggests that mutations in genes related to lysosomal function or lysosomal storage disorders may affect the risk of PD development, such as GBA1 gene mutations. In this context, recent studies have revealed the emerging role of arylsulfatase A (ASA), a lysosomal hydrolase encoded by the ARSA gene causing metachromatic leukodystrophy (MLD) in PD pathogenesis. In particular, altered ASA levels have been detected during disease progression, and reduced enzymatic activity of ASA has been associated with an atypical PD clinical phenotype, including early cognitive impairment and essential-like tremor. Clinical evidence further reveals that specific ARSA gene variants may act as genetic modifiers in PD. Recent in vitro and in vivo studies indicate that ASA may function as a molecular chaperone interacting with α-synuclein (SNCA) in the cytoplasm, preventing its aggregation, secretion and cell-to-cell propagation. In this review, we summarize the results of recent preclinical and clinical studies on the role of ASA in PD, aiming to shed more light on the potential implication of ASA in PD pathogenesis and highlight its biomarker potential.
Age-Dependent Auditory Processing Deficits after Cochlear Synaptopathy Depend on Auditory Nerve Latency and the Ability of the Brain to Recruit LTP/BDNF

Age-related decoupling of auditory nerve fibers from hair cells (cochlear synaptopathy) has been linked to temporal processing deficits and impaired speech recognition performance. The link between both is elusive. We have previously demonstrated that cochlear synaptopathy, if centrally compensated through enhanced input/output function (neural gain), can prevent age-dependent temporal discrimination loss. It was also found that central neural gain after acoustic trauma was linked to hippocampal long-term potentiation (LTP) and upregulation of brain-derived neurotrophic factor (BDNF). Using middle-aged and old BDNF-live-exon-visualization (BLEV) reporter mice we analyzed the specific recruitment of LTP and the activity-dependent usage of Bdnf exon-IV and -VI promoters relative to cochlear synaptopathy and central (temporal) processing. For both groups, specimens with higher or lower ability to centrally compensate diminished auditory nerve activity were found. Strikingly, low compensating mouse groups differed from high compensators by prolonged auditory nerve latency. Moreover, low compensators exhibited attenuated responses to amplitude-modulated tones, and a reduction of hippocampal LTP and Bdnf transcript levels in comparison to high compensators. These results suggest that latency of auditory nerve processing, recruitment of hippocampal LTP, and Bdnf transcription, are key factors for age-dependent auditory processing deficits, rather than cochlear synaptopathy or aging per se.
Summer in the city: behaviour of large gulls visiting an urban area during the breeding season

ABSTRACT Capsule: Large numbers of Herring Gulls Larus argentatus and Lesser Black-backed Gulls Larus fuscus from a traditional colony in the Netherlands visit an urban area for food in the chick rearing period, causing nuisance while doing so. Aims: To assess the potential contribution of large gulls breeding in a traditional colony to gull–human conflicts in cities. Methods: Colour-ringed gulls from a nearby colony were counted in the study area throughout the 2014 breeding season. The average numbers observed daily per species and sex were compared between different breeding phases. Results: Fluctuations in numbers of both species could be explained by breeding phase. Numbers of females of both species and male Lesser Black-backed Gulls dropped significantly during laying and incubation. Numbers peaked post-hatching, coinciding with increased engagement in nuisance events and more frequent displacements within the study area. Conclusion: Large gulls from a traditional colony frequently visited the urban study area, especially when food demand for chick provisioning was high, suggesting that city visits are motivated by accessibility of urban food. The proportion of rooftop breeding gulls in this area is low. Therefore, measures to avoid nuisance should focus on reducing food availability rather than controlling gull breeding.
Lower Trabecular Bone Score and Spine Bone Mineral Density Are Associated With Bone Stress Injuries and Triad Risk Factors in Collegiate Athletes

Determinants of bone health and injury are important to identify in athletes. Bone mineral density (BMD) is commonly measured in athletes with Female Athlete Triad (Triad) risk factors; the trabecular bone score (TBS) has been proposed to predict fracture risk independent of BMD. Evaluation of TBS and spine BMD in relation bone stress injury (BSI) risk has not been studied in female collegiate athletes.
The handwriting brain in middle-childhood.

While the brain network supporting handwriting has previously been defined in adults, its organization in children has never been investigated. We compared the handwriting network of 23 adults and 42 children (8 to 11 year old). Participants were instructed to write the alphabet, the days of the week and to draw loops while being scanned. The handwriting network previously described in adults (5 key regions: left dorsal premotor cortex, superior parietal lobule, fusiform and inferior frontal gyri, and right cerebellum) was also strongly activated in children. The right precentral gyrus and the right anterior cerebellum were more strongly activated in adults than in children while the left fusiform gyrus was more strongly activated in children than in adults. Finally, we found that, contrary to adults, children recruited prefrontal regions to complete the writing task. This constitutes the first comparative investigation of the neural correlates of writing in children and adults. Our results suggest that the network supporting handwriting is already established in middle-childhood. They also highlight the major role of prefrontal regions in learning this complex skill and the importance of right precentral regions and cerebellum in the performance of automated handwriting.