Miracle-dz/UniFAR-FTUs huggingface dataset:
The cytoplasmic domain of the AAA+ protease FtsH is tilted with respect to the membrane to facilitate substrate entry

AAA+ proteases are degradation machines that use ATP hydrolysis to unfold protein substrates and translocate them through a central pore toward a degradation chamber. FtsH, a bacterial membrane-anchored AAA+ protease, plays a vital role in membrane protein quality control. How substrates reach the FtsH central pore is an open key question that is not resolved by the available atomic structures of cytoplasmic and periplasmic domains. In this work, we used both negative stain TEM and cryo-EM to determine 3D maps of the full-length Aquifex aeolicus FtsH protease. Unexpectedly, we observed that detergent solubilization induces the formation of fully active FtsH dodecamers, which consist of two FtsH hexamers in a single detergent micelle. The striking tilted conformation of the cytosolic domain in the FtsH dodecamer visualized by negative stain TEM suggests a lateral substrate entrance between the membrane and cytosolic domain. Such a substrate path was then resolved in the cryo-EM structure of the FtsH hexamer. By mapping the available structural information and structure predictions for the transmembrane helices to the amino acid sequence we identified a linker of ∼20 residues between the second transmembrane helix and the cytosolic domain. This unique polypeptide appears to be highly flexible and turned out to be essential for proper functioning of FtsH as its deletion fully eliminated the proteolytic activity of FtsH.
AN INTEGRATED APPROACH FOR THE PROGRESSIVE DAMAGE ANALYSIS OF V-NOTCHED GLASS/EPOXY COMPOSITE LAMINATES

This work proposes an integrated approach for the progressive damage analysis of V-notched glass/epoxy composites with layups [90/0]s and [45/90/-45/0]s. The material property degradation method (MPDM) is used to model the intra-lamina failure whereas cohesive elements (CE) are employed to account for the delamination at the interfaces. Different failure theories are considered in the MPDM-CE approach and a comparative study of these failure theories is presented. The predictions are compared with the experimental results reported by Hallett and Wisnom [1]. Good agreement between experiment and simulation is obtained, showing that the MPDM-CE approach can effectively predict the progressive failure in V-notched glass/epoxy composite laminates.
МЕДИЦИНСКИЕ НАУКИ COVID-19 AND AUTOIMMUNE DISEASES : DRUGS USE AND PHYSICAL ACTIVITY EFFICIENCY OVERVIEW

A new coronavirus COVID-19 become a global health emergency. This paper touches two important topics in the context of COVID-19. First, we will essentially specify physical activity effects on immune system like defence to viral infection and upper respiratory tract infection (URTI). Second, COVID-19 is surely conditioning the treatment strategy of autoimmune disease, rheumatoid arthritis (RA), and rheumatic heart disease (RHD). Compared to the general population, the infectious risk of RA and RHD is greatly increased because the immune system of patients with autoimmune diseases, combined with the iatrogenic effect generated by corticosteroids immunosuppressive, salicylate and antibiotic drugs, are generally impaired. Thus, we analyse the evidence on either a positive or negative effect of drugs commonly used to treat RA and RHD in this particular condition, in order to optimize the current approach to RA and RHD patients. Аннотация. Новый коронавирус COVID-19 быстро стал глобальной чрезвычайной ситуацией в области здравоохранения. Эта статья затрагивает две важные темы в контексте COVID-19. Во-первых, мы по существу определим влияние физической активности на иммунную систему, такую как защита от вирусной инфекции и инфекции верхних дыхательных путей. Во-вторых, COVID-19, безусловно, обусловливает стратегию лечения аутоиммунных заболеваний, ревматоидного артрита (РА) и ревматической болезни сердца (РБС). По сравнению с общей популяцией инфекционный риск РА и РБС значительно повышен, так как иммунная система больных аутоиммунными заболеваниями в сочетании с ятрогенным эффектом, создаваемым кортикостероидами иммуносупрессивными, салицилатными и антибиотиковыми препаратами, как правило, нарушена. Таким образом, мы анализируем данные о положительном или отрицательном эффекте лекарств, обычно используемых для лечения РА и РЗС в этом конкретном состоянии, с целью оптимизации текущего подхода к пациентам с РА и РЗС.
Large eddy simulation of turbulent flows by a direction splitting solver in complex geometries

The possibility of adding a large eddy simulation turbulence model to a preexisting solver for the incompressible Navier-Stokes equations is investigated. Preliminary research into turbulence theory and into numerical modelling methods relevant to the solver in use is performed, then a set of Fortran 90 subroutines implementing a Smagorinsky-Lilly closure model is developed. The solver, with the addition of this closure model, is tested performing a simulation of the flow around a sphere at a Reynolds number of 3700. The results are then confronted with those available in the literature. The solver in use is based on the one originally proposed by Guermond and Minev, which employs finite differences on Cartesian staggered grids, Douglas direction splitting for the momentum equation and an innovative direction splitting technique for the pressure equation. It was developed for optimal scalability on a parallel architecture. The version of the software used for this work differs from the original mainly by the addition of an immersed boundary method. This allows for the placement of a body of arbitrarily complex geometry inside the computational domain, such as the sphere used for the tests peformed.
Triple Helix and Decentralization Approach: Management of School Operational Assistance Funds

Improving the quality of education can be implemented in various ways. This study aims to analyze the triple helix approach in the management of School Operational Assistance funds which are decentralized to schools. Decentralized planning, organizing, directing and controlling the process as stipulated in the Regulation of the Minister of Education and Culture of the Republic of Indonesia concerning Technical Guidelines for Regular School Operational Assistance. Data collection was carried out through questionnaires and in-depth interviews with 35 respondents, namely the principal, and the administrator of the secondary and vocational school committee in Sidenreng Rappang Regency. Use the Nvivo-12 plus program assistance to process data from interviews, documents and explanations from the Minister of Education and Culture. The results showed that the authority and deconcentration and devaluation authority dominated the process of using school operational assistance funds. The main finding of this study is the importance of the triple helix approach of universities, industry, and local government in the area of decentralization of authority for channelling school operational assistance funds. This finding is supported by optimizing the involvement of the triple helix in school committee members. The implications of the triple helix approach will ensure accountability and transparency in the management of school operational assistance funds because they are stakeholders in the education sector, especially in secondary and vocational schools.
Task repetition influence on pupil response during encoding of auditory information in normal-hearing adults

Proceedings of the International Symposium on Auditory and Audiological Research (Proc. ISAAR), Vol. 7: Auditory Learning in Biological and Artificial Systems, August 2019, Nyborg, Denmark. Edited by A. Kressner, J. Regev, J. C. -Dalsgaard, L. Tranebjærg, S. Santurette, and T. Dau. The Danavox Jubilee Foundation, 2019. © The Authors. ISSN: 2596-5522. Task repetition influence on pupil response during encoding of auditory information in normal-hearing adults
Effect of Blended NPS Fertilizer and Rhizobium Inoculation

Low available phosphorus, total nitrogen, lack of adequate information on the use of Rhizobium inoculants and NPS fertilizer are the major yield limiting factors for common bean production in the study area. Thus, the experiment was conducted at Mekdela District in 2019 (July-October), to evaluate the effect of Blended NPS fertilizer rates and Rhizobium inoculation on the yield components and yield of common bean varieties. Factors studied were three common bean varieties (Red Wolaita, Hawassa Dume, Nasir), three levels of blended NPS fertilizer rate (0, 100, 200 kg ha-1) and two levels of Rhizobium inoculation (with and without). Randomized complete block design in factorial arrangement with three replication was used. Result showed varieties had significant differences on days to 50% flowering, days to 90% physiological maturity and plant height. The highest number of days to 50% flowering (42.89) and days to 90% physiological maturity (82.56) were recorded for variety Red Wolaita. Academic Journal of Research and Scientific Publishing | Vol 2 | Issue 18 Publication Date: 5-10-2020 www.ajrsp. com 116 ISSN: 2706-6495 Similarly, NPS rates and Rhizobium inoculation had significant effect on days to 50% flowering and days to 90% physiological maturity. Significantly higher number of days to 50% flowering (42.59) was recorded from Rhizobium inoculation. The highest number of days to 90% physiological maturity (90.83) was recorded at blended NPS rate (200 kg ha1). There was significant interaction effect of NPS rates with varieties on grain yield and effective nodules. Where, the highest effective nodules per plant (19.32) and grain yield (2373 kg ha-1) were recorded from NPS rate 100 kg ha-1 of for variety Nasir. The combined application of Rhizobium inoculation with NPS rates and varieties had significant effect on number of total nodules per plant and seeds per pod. Where, the highest number of total nodules per plant (68.53) and seeds per pod (7.5) were recorded from Rhizobium inoculation with NPS rate 100 kg ha-1and for variety Nasir. Based on the results of this study, it can be tentatively concluded that Rhizobium inoculation with application of NPS rate of 100 kg ha-1 found to be appropriate for common bean variety Nasir as well as for other varieties, where variety Nasir was superior of production in the study area.
Multi-decadal climate cycles controlled by the periodic reversals of solar magnetic field polarity

1] The linkage between multi-decadal climate variability and activity of the sun has been long debated based upon observational evidence from a large number of instrumental and proxy records. It is difficult to evaluate the exact role of each of solar parameters on climate change since instrumentally measured solar related parameters such as Total Solar irradiance (TSI), Ultra Violet (UV), solar wind and Galactic Cosmic Rays (GCRs) fluxes are more or less synchronized and only extend back for several decades. Here we report tree-ring carbon-14 based record of eleven-year / twenty-two year solar cycles during the Maunder Minimum (17 century) and the early Medeival Maximum Period (9-10 century) to reconstruct the state of the sun and the flux of incoming GCRs. The result strongly indicates that the influence of solar cycles on climate is persistent beyond the period after instrumental observations were initiated. We find that the actual lengths of solar cycles vary depending on the status of long-term solar activity, and that periodicity of the surface air temperatures are also changing synchronously. Temperature variations over the twenty-two-year cycles seem, in general, to be more significant than those associated with the eleven-year cycle and in particular the case around the grand solar minima such as the Maunder AC C EP TE D M AN U SC R IP T ACCEPTED MANUSCRIPT Minimum (1645-1715AD). The polarity dependence of cooling events found in this study suggests that the GCRs can not be excluded from the possible drivers of decadal to multi-decadal climate change.
Analysis of Households Food Insecurity in the Face of Climate Variability: Evidence from North Shewa Zone, Amhara Region, Ethiopia

Food insecurity is more worrisome now than ever before due to unprecedented climate variability and widespread rural poverty. Research-based and policy relevant empirical evidence is crucial to design strategies to address food insecurity in the face of climate variability. Thus, this study examines the status of food insecurity among households’ and its determinants in North Shewa Zone of Amhara Region using cross-sectional data collected from 382 sample households. Households’ food insecurity status was determined by comparing the total calorie available for consumption per adult equivalent to the minimum level of subsistence requirement per adult equivalent of 2200 kcal. Logistic regression model was used to identify factors that influence food insecurity status of households in the study area. Accordingly, the results of the study show that majority (56.28%) of the sample households in the study area were food insecure. In addition, results revealed that age, literacy, cultivated land size, soil fertility status, number of oxen owned and irrigation water use were the major factors negatively associated with food insecurity. In contrast, sex, household size, distance to the main market and rainfall variability have increased the probability of being food insecure. The findings imply that majority of the households are food insecure where its improvement can be addressed through appropriate policy, institutional and technological options.
Determining of performances on different characteristics in Safflower (Carthamus tinctorius) genotypes under organic and conventional production systems

All over the world, organic farming has begun to attract attention in recent years. There is an increasing demand for crop cultivars specifically adapted for this system of cultivation. The main objective of this study was to determine the performance, stability of different safflower (Carthamus tinctorius L.) cultivars to reveal heritability of yield components under conventional and organic farming conditions. Yield components including seed and oil yield were significantly responded to variability in varieties and environmental conditions. Shifa, TAEK and Remzibey in seed yield; TAEK and Shifa in oil content; and Remzibey and Shifa in oil yield seemed to be high yielding and stabile varieties. Yenice was determined as low yielding and instable variety. High heritability in number of branch, 1000 seed weight, oil content, oleic acid content and linoleic acid content assigned that these characters are mostly formed by genotypic performance, whereas having low heritability, plant height, seed yield and oil yield were determined as under genotype x environment interaction.
Rokhayati et al./ Contextual Effect of “Posyandu” in the Incidence of Anemia in Children

Background: Iron deficiency can cause anemia which adversely affects children's growth, especially in terms of achievement, quality of life, and performance as human resources in the future. Birth weight, exclusive breastfeeding complementary feeding (EBF-CF) have a direct relationship with the incidence of anemia in infants. The purpose of this study is to examine the factors that influence anemia in children under five at the Banguntapan II Bantul Health Center, Yogyakarta. Subjects and Method: The cross sectional study was conducted at 25 posyandu in the working area of Banguntapan II Bantul Health Center, Yogyakarta, Indonesia. A sample of 200 children aged 24–60 months was selected by simple random sampling. The dependent variable is anemia in infants. Independent variables are exclusive breastfeeding, breastfeeding, birth weight, nutritional status (BB / TB), mother's education, and mother's work. Anemia data was measured by Hb meter, other variable data were collected using questionnaires. The data were analyzed using a multiple logistic regression with a multilevel approach. Results: The risk of anemia in infants is reduced by exclusive breastfeeding (b = –5.77; 95% CI = 11.30 to -0.24; p = 0.041), EBF-CF administration is appropriate (b = –12.24; 95% CI = -22.47 to 2.00 , p = 0.019), birth weight 2500–4000 g (b = –5.04; CI 95% = -9.87 to 0.21; p = 0.040), good nutritional status (-2SD + 2SD) (b = –9.11; CI 95% = -17.99 to 0.23; p = 0.044), high maternal education (high school) (b = -10.96; 95% CI = -20.61 to 1.31; p = 0.026), and mothers who work at home (b = -5.98; 95% CI = -11.27 to -0.68; p = 0.026). Posyandu strata showed that there was a contextual influence of posyandu on variations in anemia incidence (ICC = 62.62%). Conclusions: Exclusive breastfeeding, proper EBF-CF, normal birth weight, good nutritional status, high maternal education, and mothers working at home reduce the risk of anemia in infants.
The effects of the DTPA extraction conditions on the determination of micronutrients in Brazilian soils

Abstract Extraction steps of the DTPA procedure for copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) determinations were tested in four representative soils from the State of Sao Paulo (Brazil), namely Red‐Yellow Latosol (LV), Red‐Yellow Podzolic (PV), Dusk‐Red Latosol (LR), and Sand Soil (AQ). The results indicated that the use of volume sampling, although possible, may cause biased results. Thus, direct soil sampling in terms of mass is recommended. Variations in the pH of the extraction solution from 7.1 to 7.5 can be critical for acid soils, but the best results for the soils tested were observed by keeping the extraction solution pH at 7.3. Some sort of equilibrium seems to be reached after a soil/extraction solution shaking time of 150 min for all soils tested. No significant changes in the micronutrient concentrations are observed if the soil suspension is filtered up to 120 min after shaking, but changes may occur upon storing the filtrate for more than 7 days at temperatures of 25°C‐30°C.
A Study of Problems of Alcoholism and Substance Abuse in Adolescents of Professional College

Introduction: The term “adolescent” is an adjective describing a young person in the process of developing from a child into an adult. It is derived from the Latin verb “adolescere” which means “to grow up. ” Alcohol is the world’s third-largest risk factor for disease and contributes to 4% of the global burden of disease. It is estimated that ∼2.5 million deaths each year are directly attributable to alcohol, with 9% of deaths in the 15–29 years age group. Adolescence is the critical period when the first initiation of substance use takes place. Encouragement by peer being groups, the lure of popularity, and early availability of many such substances make an adolescent an easy prey. Early identification of adolescent risk factors may be helpful in preventing the risks. Thus, this study was taken up with the following objectives: (1) To estimate the prevalence of substance abuse among adolescents in professional college and (2) to identify the risk factors responsible for substance abuse. Materials and Methods: A cross-sectional study was conducted among the 1st year medical students. All the 1st year students (150) were included for the study. Out of 150, 114 volunteered to answer a pre-structured and tested questionnaire which was used to collect the data. Results: Out of the 150 students, 114 (76%) agreed to participate in the study. Of these 114 students, the male participants were 84.2% (n = 96) and female participants were 15.7% (n = 18). The majority of the students were Hindu (82%). Most students (97.3%; n = 111) lived in hostel. Out of the 114 participants, 91 (79.8%) revealed that they consumed alcohol. Conclusion: Peer group pressure is the key source of initiation of alcohol consumption. The development of risky behaviors such as alcohol consumption and tobacco use at young age indicates higher chances of getting addicted. Thus, there is a need to educate the youth regarding the consequences of such habits.
GeoJournal of Tourism and Geosites

In the marketing of tourist locations several strands can be exploited to stress the potentialities of a place: landscape, buildings, quality foods. European regulations connect local food to the place and to history in terms of growing or manufacturing praxis and of local traditions rooted in ways of preparing and of consuming it. In tourism, food products add to the travel experience since they can be employed to define the cultural identity of a location. This paper, while reckoning the role of quality local food products in tourism, wants to call attention to the added value British grand tourists’ reports can play in defining the historical roots of quality food items. To this aim, the brief descriptions of Sicilian prickly pears from Arthur John Strutt, a British painter and a traveller, are reported, and discussed for the first time, as a means to better characterize the PDO cactus fruits grown in Sicily.
Assessing genetic diversity of some banana cultivars using inter simple sequence repeats (ISSR) markers

The purpose of this study was to assess the genetic diversity of 12 banana cultivars using inter simple sequence repeat (ISSR) markers. Twenty ISSR primers were used for screening, seven primers produced 54 total bands and 53 polymorphic bands with an average percentage of polymorphism of 96.43%. The average polymorphism information content (PIC), the average effective multiplex ratio (EMR), the average marker index (MI) and the average resolving power (Rp) were 0.26, 7.46, 2.08 and 4.10 respectively.Genetic similarity matrix from Pearson coefficient between the cultivars ranged from 0.010 to 0.657, with an average of 0.454. The lowest was between Kluai Sao Kratueb Ho and Kluai Hin while the highest was between Kluai Hak Muk Som and Kluai Nam Wa Kom. Cluster analysis using the unweighted pair group method with arithmetic mean analysis (UPGMA) divided 12 cultivars into two major groups. The first group included the main cultivar in this study while the second group comprised of only two cultivars. Although the close relate between different genome type was found, these clustering was acceptable with Cophenetic Correlation Coefficient (CCC) was 0.82.