Miracle-dz/UniFAR-FTUs huggingface dataset:
MicroRNA expression and functional analysis in Hodgkin lymphoma

In this thesis, we studied the role of microRNAs (miRNAs) in the pathogenesis of Hodgkin lymphoma (HL). Small RNA sequencing revealed 84 significantly differentially expressed miRNAs between HL cell lines and normal germinal center B cells. Inhibition of the in HL significantly overexpressed miR-24-3p resulted in decreased growth which was at least in part caused by an increase in apoptotic cells. MiRNA target gene identification using Ago2-IP in HL cell lines revealed 1,142 miRNA target genes of which 52 were predicted to be targeted by miR-24-3p. Western blotting analysis confirmed increased CDKN1B/P27kip1 upon miR-24-3p inhibition, possibly explaining the effect on cell growth. We next set up a next generation sequencing based high throughput loss- and gain-of-function screening approach to identify miRNAs that influence HL cell growth. The overexpression screen revealed that miR-19b-1 may enhance while miR-141 may repress HL cell growth. The inhibition screen revealed that inhibition of miR-449a-5p, miR-625-5p, let-7f-2-3p and miR-21-5p has a negative effect on HL cell growth. The highly abundant miR-21-5p showed significantly higher expression levels in HL. We confirmed the negative effects of miR-21-5p inhibition on cell growth and observed a concomitant significant increase in apoptotic cells. Among the in HL Ago2-IP enriched target genes, we identified 36 predicted miR-21-5p targets. We confirmed targeting of BTG2 and PELI1 by miR-21-5p using reporter assays and Western blot. Overall, we set up the technology for functional miRNA studies in HL and identified two miRNAs and their target genes relevant for the pathogenesis of HL.
Are mega-events a solution to address physical inactivity? Interrogating the London 2012 Paralympic sport participation legacies among people with disabilities

Abstract Understanding why non-active people with disabilities have not participated in more sport since the London 2012 Paralympic Games is the focus of this paper. This research reviews the constraints preventing people with disabilities from participating in more sport, and the role the London 2012 Paralympic Games plays in this. Eighty-one people with disabilities in England who are non-active completed an online questionnaire exploring their constraints to participating in more sport. The data suggests attitudes towards the London 2012 Paralympic Games were not an important reason for the lack of sport participation: instead, systemic and social barriers are more constraining to participation in more sport. A factor analysis identified four components that constrain non-active people with disabilities: sport provision; economic; unawareness of how to include people with disabilities in sporting activities; and access to sport participation opportunities. The four components explained 72.93% of the total variance. It is recommended mega sporting events are not used as a policy intervention to increase sport participation of people with disabilities, as this does not account for constraining social and systemic barriers to sports participation. Instead, bottom-up solutions designed and managed in conjunction with people with disabilities may be more effective.
Fixed or mixed? Variation in tree functional types and vegetation structure in a forest-savanna ecotone in West Africa

Abstract We analysed thirty-five 400-m2 plots encompassing forest, savanna and intermediate vegetation types in an ecotonal area in Ghana, West Africa. Across all plots, fire frequency was over a period of 15 years relatively uniform (once in 2–4 years). Although woodlands were dominated by species typically associated with savanna-type formations, and with forest formations dominated by species usually associated with closed canopies, these associations were non-obligatory and with a discrete non-specialized species grouping also identified. Across all plots, crown area index, stem basal area and above-ground biomass were positively associated with higher soil exchangeable potassium and silt contents: this supporting recent suggestions of interplays between potassium and soil water storage potential as a significant influence on tropical vegetation structure. We also found an average NDVI cover increase of ~0.15% year−1 (1984–2011) with plots dominated by non-specialized species increasing more than those dominated by either forest- or savanna-affiliated species. Our results challenge the traditional view of a simple forest vs. savanna dichotomy controlled by fire, and with our newly identified third non-specialized species grouping also potentially important in understanding ecotonal responses to climate change.
A retrospective study of blunt trauma abdomen in a tertiary center in central India: evaluation, management and outcome

Background: Blunt abdominal trauma is fairly common emergency and it is one of the important components of polytrauma. It requires high degree of suspicion, investigation and management. Inspite of improved imaging techniques leading to early recognition it is still associated with high morbidity and mortality. Trauma is the leading cause of blunt abdominal injury. This aim of the study was to find etiology, early diagnosis and management of patients with blunt abdominal trauma. Methods: This a retrospective study conducted in Gandhi medical college, Bhopal in which 90 cases of blunt abdominal trauma presented to emergency and outpatient department were included in the study duration of January 2019 to December 2019. Results: Motor vehicle accident was the most common mode of injury. Liver being the most common visceral organ injured while the most common surgery performed was the repair or resection and anastomosis of hollow viscous perforation. Rib fracture was the most common extra abdominal injury seen in 17.7% cases. Mortality rate was 5.5%. Most of the liver, spleen and renal injuries can be managed non-operatively whereas hollow viscous injury needs laparotomy. Conclusions: The result of present study is similar to other studies. Rapid diagnosis, early and timely referral, adequate and trained staff, close and careful monitoring, early wise and skilled decision to go for operative or non-operative management can help save many lives.
Character Education at Sekolah Alam Minangkabau and Sekolah Alam Ar-Royyan Padang

This research discloses how character education builds students’ character at SekolahAlam Minangkabau and SekolahAlamAr-Royyan Padang, so students not only have knowledge capabilities but also provide character social attitudes. This is a qualitative typified research. The data were collected through observation, interviews, and document analysis. The validity of the data was examined through the triangulation method while Miles and Huberman’s theory of analysis were used in analyzing the data. The results of the research suggest that: The characters of the students in both schools are religious, tolerant, obedient, diligent, creative, independent, curious, communicative, ecofriendly, and responsive. The students’ character is built through macro and micro strategies. The varieties of the character education used in both schools include the curriculum, facility, educational program, extracurricular as well as the time and the way the character education is implemented. The students’ parents view the character education as a support for them in developing their children’s personality,itinfluences the students’ daily activities outside the schools,and moreover, it alsoestablishes a good communication between the teacher and the students’ parents.
Phosphorus and Zinc Fertilization Improve Productivity and Profitability of Rice Cultivars under Rice-Wheat System

Continuous cropping of rice (Oryza sativa L.) and wheat (Triticum aestivum L.) deplete soil fertility, reduced crop productivity, and grower’s income. Phosphorus (P) and zinc (Zn) deficiencies are considered important nutritional constraints under rice-wheat cropping system. One strategy to increase crop productivity and grower’s income under the rice-wheat system is the balanced application of P and Zn fertilizers. The objective of this research was to evaluate the impact of sole and various combinations of P (0, 40, 80, and 120 kg ha−1) and Zn levels (0, 5, 10, and 15 kg ha−1) on productivity (grain yield and yield components) and profitability (net returns) of different rice genotypes (cultivars) (fine (cv.Bamati-385) vs. coarse (cv. Fakhre-e-Malakand and cv. Pukhraj)) and their residual effect on the income of the succeeding wheat crop (cv. Siran-2010). The results revealed that both rice and wheat productivity and profitability was higher with the combined application of both nutrients at higher rates (80 and 120 kg P ha−1 and 10 and 15 kg Zn ha−1). The highest productivity and profitability was obtained with the cultivation of hybrid rice “Pukhraj” (Pukhraj > Fakhre-e-Malakand > Basmati-385). It was concluded from this study that application of higher P and Zn levels and growing of hybrid rice increased productivity and profitability under the rice-wheat cropping system.
Migration and business cycles: testing the OCA theory predictions in the European union

ABSTRACT The labour mobility is one of the main criteria of the optimum currency areas theory, that formed a basis for the business cycle synchronization research in the last 20 years. However, the link between migration and business cycle comovement has not been established previously due to the lack of data. Using the newly available database on bilateral migration flows, this article fills the gap in the existing literature. The results from the Bayesian model averaging of dynamic panels with weakly exogenous regressors show the existence of a strong link between bilateral migration and business cycle comovement. These results are in line with the predictions of the optimum currency areas theory. Moreover, the relative importance of migration flows is higher than others well-established determinants of business cycle synchronization. Consequently, the results show that the European Union is moving closer to becoming an optimum currency area.
Identifying Sustainable Grassland Management Approaches in Response to the Invasive Legume Lespedeza cuneata: A Functional Group Approach

We propose combining the filter framework model of community assembly with the passenger-driver model of non-native species behavior to help clarify the impacts of invasive species in the communities they invade and to guide sustainable management protocols. Observational field surveys and a greenhouse experiment explored the role of the invasive legume Lespedeza cuneata in the communities it invades and how natives in three functional groups—grasses, forbs, and legumes—respond to its presence. Within-site analyses from the field survey revealed differences in invaded and uninvaded areas in half of the sites, suggesting that site-specific characteristics influences whether L. cuneata’s presence corresponds to local differences in species composition. The greenhouse experiment found higher levels of saprophytic and arbuscular mycorrhizal fungi in soil conditioned by L. cuneata than in unconditioned soil. However, competition between L. cuneata or the native congener L. capitata and nine native species illustrated stronger aboveground competitive effects than belowground soil effects due to soil conditioning, with impacts differing among functional groups. The response of L. cuneata was reduced in the presence of grasses and other legumes but not forbs. Assessing the impact of L. cuneata with the combined community assembly model revealed this invasive plant acts as a driver because it alters abiotic and biotic filters to impact species composition. Managing for high grass abundance and planting native legumes will help sustain grasslands from L. cuneata invasion.
Theranostic Nanoplatforms of Thiolated Reduced Graphene Oxide Nanosheets and Gold Nanoparticles

In this study, graphene oxide (GO) and reduced-thiolated GO (rGOSH) were used as 2D substrate to fabricate nanocomposites with nanoparticles of gold nanospheres (AuNS) or nanorods (AuNR), via in situ reduction of the metal salt precursor and seed-mediated growth processes. The plasmonic sensing capability of the gold-decorated nanosheets were scrutinized by UV-visible (UV-VIS) spectroscopy. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analyses (TGA), and atomic force microscopy (AFM) were performed in order to prove the actual reduction that occurred concomitantly with the thiolation of GO, the increase in the hydrophobic character as well as the size, and preferential gathering of the gold nanoparticles onto the nanosheet substrates, respectively. Moreover, the theoretical electronic and infrared absorption (UV-VIS and IR) spectra were calculated within a time-dependent approach of density functional theory (DFT). Eventually, in vitro cellular experiments on human neuroblastoma cells (SH-SY5Y line) were carried out in order to evaluate the nanotoxicity of the nanocomposites by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) colorimetric assay. Results pointed out the promising potential of these hybrids as plasmonic theranostic platforms with different hydrophilic or hydrophobic features as well as cytotoxic effects against cancer cells.
The Relationship of Knowledge and Attitudes of Young Women With Personal Hygiene Behavior When Menstruating

: Personal Hygiene is an action to maintain the cleanliness and health of a person in maintaining good physical and psychological health. The lack of knowledge of adolescent girls about Personal Hygiene during menstruation will have an impact on the attitudes of young women in behaving to maintain their reproductive health. Objective: The purpose of this study was to analyze the relationship between attitudes and knowledge of adolescent girls with personal hygiene behaviors. Method: The research design used in this study was correlation using a cross sectional approach with a total sample of 51 people. Results: The results showed that knowledge in the good category was 39.2%, attitudes in the good category were 56.9% and behavior in the good category was 56.9%. Simpullan is a relationship between attitude and knowledge with personal hygiene behavior during menstruation.
Universal Scaling of Thin Current Sheets

Thin current sheets (TCSs) with thicknesses about ion Larmor radii are widespread in space. It is important to describe their equilibrium structure allowing them to store and then explosively release the accumulated free energy. When ions are moving along quasi‐adiabatic trajectories while magnetized electrons follow guiding center drift orbits, TCSs can be described within the framework of a hybrid approach. The thickness of the embedded electron sheet remains uncertain because of the scale‐free character of electron motion. In this work, we propose a novel analytical model of the multilayer TCS that provides a universal expression describing the inner (embedded) electron sheet in dependence of TCS characteristics. An unusual property of the embedded electron layer revealed in this analysis is the nonlinear profile of the magnetic field in the inner layer: B(z) ~ z1/3, which conforms excellently with MAVEN observations of 43 TCSs in the Martian magnetotail.
The study of nuclear structure of neutron-rich 81Ge and its contribution in the r-process via the neutron transfer reaction 80Ge(d,p)

The study of low-lying levels of nuclei near closed shells not only elucidates the evolution of nuclear shell structure far from stability, but also affects estimates of heavy element nucleosynthesis in supernova explosions. Especially, the properties of the low-lying levels in Ge[Germanium 81] are important because the sensitivity study of the r-process pointed out that the properties of the nucleus can affect the final abundance pattern. Also, the spins and parities measurements of the states are essential to understand the shape coexistence in odd-mass N = 49 isotones. This work describes the study of the odd-mass N = 49 nucleus, Ge in the region of neutron magic number N = 50 using an inverse kinematics (d,p) transfer reaction. The d(Ge[Germanium 80],p)Ge neutron transfer reaction was measured by bombarding a 174 μg[microgram]/cm[centimetersquared] (CD2)n[deuterated polyethylene] target with a 310 MeV beam of radioactive Ge at the Holifield Radioactive Ion Beam Facility (HRIBF) at Oak Ridge National Laboratory (ORNL). The new ORNL DAQ ASICs system was commissioned for a first implementation of the development in the experiment. The excitation energies of low-lying levels were measured. From analysis of the observed angular distributions, the spins and the parities were determined, and spectroscopic factors were extracted for the first time. Neutron capture cross sections on Ge have been calculated in a direct-semidirect model using extracted spectroscopic factors. Furthermore, two intruder states were confirmed and their properties were compared with other odd-mass N = 49 isotones.
EFL Students Perspective towards Online Learning Barriers and Alternatives Using Moodle/Google Classroom during COVID-19 Pandemic

Covid-19 pandemic has made many countries adapt on new situations in different sectors including education. The Indonesia government has decided to adjust the education mode from face-to-face to online meeting using various learning management systems (LMS) such as moodle and google classroom. Moreover, the present research depicted the online learning barriers faced by students as well as their alternatives to cope them. The research implemented descriptive mixed-method survey design. The participants were 25 students of English Education Department. The instruments used to gather the data were the questionnaires and interview regarding the topics. The results showed that students experienced three barriers during the online learning including infamiliriaty of e-learning, slow internet connection, and physical condition e.g. eye strain. The alternatives they proposed were providing training to implement the LMS before the real class, converting high-definition or big-size files into smaller one, and giving break during the online class. The conclusion stated that students had to be creatives to find any solutions and innovations regarding learning barriers including maintaining good communication with teacher and understanding best learning styles individually
Genetic Diversity among Lathyrus ssp. Based on Agronomic Traits and Molecular Markers

Grasspea (Lathyrus sativus L.) and its relatives are considered resilient legumes due to their high ability to cope with different stresses. In this study, the genetic diversity of three Lathyrus species (L. sativus, L cicera and L. ochrus) was assessed by agronomic traits and molecular markers (Simple Sequence Repeat-SSR) in order to detect accessions useful for future breeding strategies. Phenotypic traits showed a high significant variation in which 1000 seed weight (1000 SW) and protein content appeared the most discriminant, as observed by principal component analysis (PCA). SSR analysis was able to detect forty-eight different alleles with an average of 9.6 allele per locus, and a Polymorphic Information Content (PIC) and a gene diversity of 0.745 and 0.784, respectively. Cluster analysis based on agronomic traits as well as molecular data grouped accessions by species but not by geographical origin. This result was confirmed by Principal Coordinates Analysis (PCoA) and Structure Analysis as well. Moreover, genetic structure analysis revealed a high genetic differentiation between L. ochrus and the other species. Analysis of MOlecular Variance (AMOVA) displayed a greater genetic diversity within species (77%) than among them (23%). Finally, a significant positive correlation was observed between agronomic and genetic distances (Mantel’s test). In conclusion, the variability detected within accessions in each species and the differences among species may be useful to plan next breeding programs, focusing on biomass production as well as protein content.
External diffusion of B2B e-procurement and firm financial performance: role of information transparency and supply chain coordination

PurposeThe purpose of this paper is to understand the process through which external diffusion of business-to-business (B2B) e-procurement impacts firm performance. The research model has been developed to empirically examine the role of information transparency and supply chain coordination in improving the firm financial performance by external diffusion of e-procurement.Design/methodology/approachThe survey is conducted in India with a target population of purchasing professionals working on the B2B e-procurement platform. The measurement model was first tested by using confirmatory factor analysis for reliability and validity, then structural equation modeling (SEM) was used to test the hypotheses of the research model using AMOS 22. The phantom model approach has been used for testing multiple mediators.FindingsThe result of the study highlights the importance of information transparency and supply chain coordination in enhancing the firm financial performance by external diffusion of e-procurement. The results establish the role of information transparency in enhancing firm performance by improving supply chain coordination. The results also indicate that supply chain coordination mediates the relationship between external diffusion of e-procurement and firm financial performance.Originality/valueThis is the first study that has focused on the external diffusion of e-procurement and its impact on firm performance. Also, this study attempted to understand the process through which external diffusion of e-procurement impacts the firm financial performance.
Effects of dump site soil on the leaf structures of Luffa cylindrical (sponge gourd) and Amaranthus viridis (green amaranth)

Previous researches have shown the presence of heavy metals (HMs) such as lead (Pb) at different levels in Olusosun dumpsite, Lagos. This study aimed at finding out if the anatomical differences found in Luffa cyclindrical and Amaranthus viridis grown on the dumpsite is an indication of the HMs and also determine the distribution of HMs on the dumpsite. The results showed the HMs detected in the dumpsite soil at three spots were significantly higher than the control (p=0.5). The concentrations of HMs were in sequence for soils A: Pb>Zn=Cr>Fe>Cu>Ni. Spot B: Fe>Cr>Pb>Zn>Cu>Ni, spot C: Fe>Pb>Cr>Zn>Cu>Ni and Control: Pb>Fe>Zn>Cu>Ni. All the HMs detected at the three spots and control were below the World Health Organization (WHO) and Dutch pollutant standard level, except for (Zn) which was at the exact limit (50.000Mg/kg). The plants obtained from both the dumpsite and control sites didn’t show marked visible morphological changes. However, anatomical changes in the leaves epidermis such as irregular shape of the epidermis, absence of trichomes and decrease in quality and stomata size were all witnessed in the dumpsite plant as against the control. Hence, it is likely that all detected differences in the epidermal structures of the test plant grown on dumpsite soil were caused by the high level of HMs present in the dumpsites and therefore suggested that, changes in epidermal structures of L. cyclindrical and A. viridis grown on Olusosun dumpsite soils is an indication of HMs such as Zn and Pb present. Keywords: Heavy metals, Anatomical changes, Morphological changes, Toxicity
Sicana odorifera “Kurugua” from Paraguay, Composition and Antioxidant Potential of Interest for the Food Industry

Summary: The aim of this study was to evaluate the physicochemical characteristics, centesimal composition and antioxidants of the Sicana odorifera pulp and the antioxidant potential of the seeds and fruit peel harvested in a culture of the city of San Lorenzo, Paraguay. These fruits harvested in Paraguay present an antioxidant potential, interesting for food industry, especially in a ripe and semi-ripe state, where the highest content of vitamin C and total phenols was observed, as well as the total antioxidant capacity (ABTS).