Miracle-dz/UniFAR-FTUs huggingface dataset:
Nucleic Acid Preservation Card Surveillance Is Effective for Monitoring Arbovirus Transmission on Crocodile Farms and Provides a One Health Benefit to Northern Australia

The Kunjin strain of West Nile virus (WNVKUN) is a mosquito-transmitted flavivirus that can infect farmed saltwater crocodiles in Australia and cause skin lesions that devalue the hides of harvested animals. We implemented a surveillance system using honey-baited nucleic acid preservation cards to monitor WNVKUN and another endemic flavivirus pathogen, Murray Valley encephalitis virus (MVEV), on crocodile farms in northern Australia. The traps were set between February 2018 and July 2020 on three crocodile farms in Darwin (Northern Territory) and one in Cairns (North Queensland) at fortnightly intervals with reduced trapping during the winter months. WNVKUN RNA was detected on all three crocodile farms near Darwin, predominantly between March and May of each year. Two of the NT crocodile farms also yielded the detection of MVE viral RNA sporadically spread between April and November in 2018 and 2020. In contrast, no viral RNA was detected on crocodile farms in Cairns during the entire trapping period. The detection of WNVKUN and MVEV transmission by FTATM cards on farms in the Northern Territory generally correlated with the detection of their transmission to sentinel chicken flocks in nearby localities around Darwin as part of a separate public health surveillance program. While no isolates of WNVKUN or MVEV were obtained from mosquitoes collected on Darwin crocodile farms immediately following the FTATM card detections, we did isolate another flavivirus, Kokobera virus (KOKV), from Culex annulirostris mosquitoes. Our studies support the use of the FTATM card system as a sensitive and accurate method to monitor the transmission of WNVKUN and other arboviruses on crocodile farms to enable the timely implementation of mosquito control measures. Our detection of MVEV transmission and isolation of KOKV from mosquitoes also warrants further investigation of their potential role in causing diseases in crocodiles and highlights a “One Health” issue concerning arbovirus transmission to crocodile farm workers. In this context, the introduction of FTATM cards onto crocodile farms appears to provide an additional surveillance tool to detect arbovirus transmission in the Darwin region, allowing for a more timely intervention of vector control by relevant authorities.
Systemic D1‐R and D2‐R antagonists in non‐human primates differentially impact learning and memory while impairing motivation and motor performance

Dopamine (DA) modulates cognition in part via differential activation of D1 and D2 receptors within the striatum and prefrontal cortex, yet evidence for cognitive impairments stemming from DA blockade or deficiency is inconsistent. Given the predominance of D1 over D2 receptors (R) in the prefrontal cortex of primates, D1‐R blockade should more strongly influence frontal executive function (including working memory), while D2‐R blockade should impair processes more strongly associated with the dorsal striatum (including cognitive flexibility, and learning). To test how systemic DA blockade disrupts cognition, we administered D1‐R and D2‐R like antagonists to healthy monkeys while they performed a series of cognitive tasks. Two selective DA receptor antagonist drugs ( SCH‐23390 hydrochloride : D1/D5‐R antagonist; or Eticlopride hydrochloride : D2/D3‐R antagonist) or placebo (0.9% saline) were systemically administered. Four tasks were used: (1) ‘visually guided reaching’, to test response time and accuracy, (2) ‘reversal learning’, to test association learning and attention, (3) ‘self‐ordered sequential search’ to test spatial working memory, and (4) ‘delayed match to sample’ to test object working memory. Increased reach response times and decreased motivation to work for liquid reward was observed with both the D1/D5‐R and D2/D3‐R antagonists at the maximum dosages that still enabled task performance. The D2/D3‐R antagonist impaired performance in the reversal learning task, while object and spatial working memory performance was not consistently affected in the tested tasks for either drug. These results are consistent with the theory that systemic D2/D3‐R antagonists preferentially influence striatum processes (cognitive flexibility) while systemic D1/D5‐R administration is less detrimental to frontal executive function.
Early intervention in psychosis: An analysis of the characteristics and service needs of patients over the age of 35

In 2016 NICE updated its guidance, extending the age to which early intervention in psychosis (EIP) services treat patients following their first episode of psychosis, to 65 years, from the previous cut‐off of 35 years. The aim of this service evaluation was to identify differences in the demographic details, diagnoses, and treatments between patients below and above 35 years to identify any unmet needs in the latter age category.
Likelihood to Recommend (L2R) Prediction using Quality of Experience (QoE) Measurements: A Longitudinal Study

— Models that predict satisfaction with a service over time need to consider the impact of emotions and remembered quality of experience in creating attitudes towards a service. However, prior research on subjective quality of experience has typically focused on experiments conducted in a single session or over a short period of time. Thus, there is a gap between our understanding of instantaneous quality of experience and long-term judgments, such as overall satisfaction and likelihood to recommend and likelihood to churn. The goal of the study in this paper was to carry out a longitudinal study that would provide initial insights into how experiences of service quality over time are mediated through emotions and memory and accumulated into longer term attitudes about the service. The longitudinal study was carried out over a period of roughly 4 weeks with around 3 sessions per week. A specially constructed online service was used where participants could select YouTube videos to view, and the service would randomly add impairments to the videos before playing back the videos and then asking questions relating to Quality of Experience, Technical Quality and overall frustration and satisfaction. In this paper, we report on the results obtained from the first 8 sessions of data.
Enhanced healing efficacy of an optimized gabapentin-melittin nanoconjugate gel-loaded formulation in excised wounds of diabetic rats

Abstract The present study aimed to design and optimize, a nanoconjugate of gabapentin (GPN)-melittin (MLT) and to evaluate its healing activity in rat diabetic wounds. To explore the wound healing potency of GPN-MLT nanoconjugate, an in vivo study was carried out. Diabetic rats were subjected to excision wounds and received daily topical treatment with conventional formulations of GPN, MLT, GPN-MLT nanoconjugate and a marketed formula. The outcome of the in vivo study showed an expedited wound contraction in GPN-MLT-treated animals. This was confirmed histologically. The nanoconjugate formula exhibited antioxidant activities as evidenced by preventing malondialdehyde (MDA) accumulation and superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymatic exhaustion. Further, the nanoconjugate showed superior anti-inflammatory activity as it inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). This is in addition to enhancement of proliferation as indicated by increased expression of transforming growth factor-β (TGF- β), vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor receptor-β (PDGFRB). Also, nanoconjugate enhanced hydroxyproline concentration and mRNA expression of collagen type 1 alpha 1 (Col 1A1). In conclusion, a GPN-MLT nanoconjugate was optimized with respect to particle size. Analysis of pharmacokinetic attributes showed the mean particle size of optimized nanoconjugate as 156.9 nm. The nanoconjugate exhibited potent wound healing activities in diabetic rats. This, at least partly, involve enhanced antioxidant, anti-inflammatory, proliferative and pro-collagen activities. This may help to develop novel formulae that could accelerate wound healing in diabetes.
Predictors of personal depression stigma in medical students in China: differences in male and female groups

ABSTRACT Depression is common worldwide, and stigmatizing attitudes toward depression have proved to be one of the major barriers to seeking professional help. The purpose of this study was to evaluate the level of personal depression stigma and identify its predictive factors among medical students in Hainan, China, as well as explore the gender difference. A total of 2,186 medical students were recruited using stratified random cluster sampling and interviewed by structured anonymous questionnaires. Personal stigma was measured by the standardized Depression Stigma Scale (DSS). Multivariate linear regression models were used to identify predictors of stigma, and the interactions between gender and each predictor were included to test its gender difference. The mean score on DSS Scale was 13.71 ± 5.35, with males significantly higher than females (14.85 vs 12.99, P < 0.0001). Compared to females, males were more likely to agree with ‘I would not vote for a class cadre if I knew they had been depressed’ and ‘I would not make friends with him if I knew he had been depressed’. Multivariate linear regression analysis revealed that males’ personal stigma was predicted by being only child (ß = 1.01, P = 0.0083), moderate-to-severe depression (ß = 1.12, P = 0.0302), and lower self-rated academic core competitiveness (Competitive: ß = 1.29, P = 0.0088, Not at all/Somewhat competitive: ß = 1.04, P = 0.0381), while females’ personal stigma was only associated with moderate-to-severe depression (ß = 1.75, P < 0.0001). Significant interactions were found between gender and self-rated academic core competitiveness. Stigmatizing attitudes toward depression were prevalent among Chinese medical students, especially male students. Gender differences were found in the predictors of stigma. Effective measures must be taken to reduce the stigma of mental health among Chinese medical students.
Durvalumab Plus Olaparib in Previously Untreated, Platinum-Ineligible Patients With Metastatic Urothelial Carcinoma: A Multicenter, Randomized, Phase II Trial (BAYOU)

PURPOSE Homologous recombination repair gene mutations (HRRm) are common in urothelial carcinoma (UC), rendering tumor cells sensitive to poly (ADP-ribose) polymerase (PARP) inhibition. We assessed efficacy and safety of durvalumab (anti–programmed cell death ligand-1) plus olaparib (PARP inhibitor) in patients with metastatic UC (mUC). METHODS This randomized, multicenter, double-blind, phase II trial enrolled untreated, platinum-ineligible patients with mUC. Patients (N = 154) were randomly assigned 1:1 to receive durvalumab (1,500 mg intravenously once every 4 weeks) plus olaparib (300 mg orally, twice daily) or durvalumab plus placebo. The primary end point was progression-free survival (PFS) assessed by investigators per RECIST version 1.1. Secondary end points included overall survival in all patients and PFS in patients with HRRm. RESULTS Overall, median PFS was 4.2 months (95% CI, 3.6 to 5.6) for durvalumab plus olaparib and 3.5 months (95% CI, 1.9 to 5.1) for durvalumab plus placebo (hazard ratio [HR], 0.94; 95% CI, 0.64 to 1.39; log-rank P value, .789). Median overall survival was 10.2 months (95% CI, 7.0 to 13.9) and 10.7 months (95% CI, 7.2 to 17.3), respectively (HR, 1.07; 95% CI, 0.72 to 1.61). In the 20% of patients with HRRm, median PFS was 5.6 months (95% CI, 1.9 to 8.1) and 1.8 months (95% CI, 1.7 to 2.2), respectively (HR, 0.18; 95% CI, 0.06 to 0.47). Treatment-related grade 3 or 4 adverse events occurred in 18% and 9% of patients, respectively. CONCLUSION Adding olaparib to durvalumab did not improve survival outcomes in an unselected mUC population. Efficacy outcomes with durvalumab were similar to those reported for other anti–programmed cell death-1/programmed cell death ligand-1 agents. However, the results of secondary analyses suggest a potential role for PARP inhibition in patients with UC harboring HRRm.
Constituent Input in the Process of Developing the Third Edition of the Civil Engineering Body of Knowledge (CEBOK3)

professional multiple research assessment of professional ethics, teaching and learning in engineering education, non-verbal communication in the classroom, and learning through historical engineering accomplishments. a signiﬁcant number of journal articles and book chapters on these topics. Abstract A careful and inclusive process was used to determine the skills, knowledge, and attitudes that future civil engineers should possess, as articulated in an updated edition of the American Society of Civil Engineers (ASCE) Civil Engineering Body of Knowledge (CEBOK3). A key component was obtaining input from various constituents at multiple points in the process. This paper focuses on three structured surveys that were developed and deployed in winter 2017, fall 2017, and spring 2018. The first survey focused primarily on the importance of the 24 outcomes in the CEBOK2 and additional potential outcomes. The second survey was a pre-draft of CEBOK3 rubrics for the cognitive domain of 21 outcomes and affective domain for 7 outcomes; respondents rated the minimum level of achievement appropriate for all civil engineers as they enter into the practice of civil engineering at the professional level. The final survey on the draft CEBOK3 allowed individuals to provide feedback on those among the 21 outcomes that held the most personal interest. The paper describes the survey development, distribution, and responses for each of the three surveys. Discussion will focus on how the survey helped inform the process of preparing a BOK that was inclusive of the ASCE membership and other stakeholders.
Staring Spells: How to Distinguish Epileptic Seizures from Nonepileptic Staring

Objective To determine the nature of staring spells and factors distinguishing epileptic from nonepileptic staring spells, we studied the clinical and demographic features of children with staring spells referred to a regional new-onset seizure clinic. Study design Our retrospective chart review encompassed 2818 consecutive patients evaluated in the new-onset seizure clinic between September 22, 2015, and March 19, 2018. We identified 121 patients with newly presenting staring spells. Results Sixty-two of 121 (51%) children were diagnosed with nonepileptic staring spells and 59 (49%) with epileptic seizures (24 with absence epilepsy, 35 with focal epilepsy). Patients with nonepileptic staring spells were younger (4.8 vs 7.1 years, P = .001) and more likely to have developmental delay (P = .005) than the seizure group. There was an 8.9-month delay on average from the onset of staring spells to the new-onset seizure clinic visit. The emergency department was a referral source for 80% (28/35) of focal seizures. In children with focal seizures, the staring spells typically lasted >1minute (29/35, 83%), whereas only 19 of 62 (31%) of children with nonepileptic staring spells had events lasting this long (P = .04). All children had a routine electroencephalography (EEG) on the day of new-onset seizure clinic visit. EEG was diagnostic in 100% (24/24) of absence seizures and 51% (18/35) of focal seizures. Conclusions In children presenting with staring spells, the differential diagnosis of epileptic staring spells vs nonepileptic staring spells can be made by history and routine EEG. Staring was as likely to be epileptic as nonepileptic spells. Younger children with developmental delay were more likely to have nonepileptic events. Our simple approach based on event duration, postictal symptoms, and EEG allowed identification of epileptic staring on first visit to new-onset seizure clinic but requires validation in future prospective studies including long-term video EEG monitoring and follow-up.
A systematic review of the effect of running shoes on running economy, performance and biomechanics: analysis by brand and model

ABSTRACT This systematic review aims to synthesise the effects of current shoe models in each shoe category and their specific features on running economy, performance and biomechanics. Electronic databases such as Web of Science, SPORTDiscuss, PubMed and Scopus were used to identify studies from 2015 to date. Due to the existing lack of consensus to define running shoes, only studies that specified the shoe brand and models used to assess their effect over runners with a certain level of fitness and training routine were included. Quality assessment of cross-sectional and intervention studies was conducted by three independent raters using a modified version of the Quality Index and the PEDro scale, respectively. A total of 36 articles were finally included, involving the analysis of 61 different shoe models over 10 different topics (i.e., running economy, running performance, spatiotemporal parameters, ground reaction forces, joint stiffness, achilles tendon, plantar pressure, tibiofemoral load, foot strike pattern and joint coordination). With this review, runners and practitioners in the field that are concerned about selecting a suitable shoe for performance, training, or injury prevention functionality have clear information about the effects of the current shoe models and their specific features.
Perinatally Human Immunodeficiency Virus–Infected Adolescents and Young Adults Demonstrate Distinct BNT162b2 Messenger RNA Coronavirus Disease 2019 Vaccine Immunogenicity

Abstract Background Immunization of vulnerable populations with distinct immunity often results in suboptimal immunogenicity, durability, and efficacy. Methods Safety and immunogenicity profiles of BNT162b2 messenger RNA coronavirus disease 2019 (COVID-19) vaccine, among people living with human immunodeficiency virus (HIV), were evaluated in 28 perinatally HIV-infected patients under antiretroviral therapy (ART) and 65 healthy controls (HCs) with no previous history of COVID-19. Thus, we measured severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–specific humoral and CD4+ T cell responses. Samples were collected before vaccination (baseline, day [D] 0), at the second dose (D21), and at 4 weeks (D28) and 6 months (D180) after D0. Proteomic profiles at D0 and D28 were assessed with a multiplexed proximity extension assay (Olink) on plasma samples. Results All HIV-infected patients mounted similar anti–SARS-CoV-2 humoral responses to those of HCs, albeit with lower titers of anti-trimeric S at D28 (P = .01). Only peripheral blood mononuclear cells of HIV-infected patients demonstrated at D28 an impaired ability to expand their specific (CD40L+) CD4+ T-cell populations. Similar humoral titers were maintained between the 2 groups at 6-months follow-up. We additionally correlated baseline protein levels to either humoral or cellular responses, identifying clusters of molecules involved in immune response regulation with inverse profiles between the 2 study groups. Conclusions Responses of ART-treated HIV-infected patients, compared to those of HCs, were characterized by distinct features especially within the proteomic compartment, supporting their eligibility to an additional dose, similarly to the HC schedule.
Short‐term control of diet affects cisplatin‐induced acute kidney injury through modulation of mitochondrial dynamics and mitochondrial GSH

Obesity affects acute kidney injury (AKI) induced by various clinical settings, including transplantation and cisplatin‐cancer therapy. However, the effect of short‐term food intake change remains to be defined. Here, we investigated the effects of short‐term high‐fat diet intake and food restriction on cisplatin‐induced AKI. Mice were fed either a high‐fat diet (HFD) or a low‐fat diet (LFD) for 11 days or were not fed for 40 hh (fasting), before cisplatin administration. Cisplatin‐induced functional and structural damages to kidneys in both HFD‐ and LFD‐fed mice, with greater damages in HFD‐fed mice than LFD‐fed mice. HFD decreased mitochondrial total glutathione (tGSH) level, along with increases in the plasma and kidney cholesterol levels. Cisplatin caused the increase of kidney cholesterol levels and oxidative stress, along with the decrease of mitochondrial tGSH levels. In addition, cisplatin‐induced mitochondrial damage and apoptosis of tubular cells in both HFD‐ and LFD‐fed mice. An increase of Fis1 (mitochondria fission 1 protein), whereas a decrease of Opa1 (mitochondria fusion 1 protein) occurred by cisplatin. These cisplatin effects were greater in HFD‐fed mice than in LFD‐fed mice. Administration of mitochondria‐specific antioxidant treatment during HFD feeding inhibited these cisplatin‐induced changes. Fasting for 40 h also significantly reduced the cisplatin‐induced changes mentioned above. These data demonstrate that short‐term HFD intake worsens cisplatin‐induced oxidative stress by the reduction of mitochondrial tGSH, resulting in increased cisplatin‐induced nephrotoxicity. These data newly indicate that the control of calorie intake, even for a short period, affects kidney susceptibility to injury. Although most studies described the effects of a long‐term high‐fat diet on the kidneys, in this study, we found that even if a high‐fat diet was consumed for a short‐term, physiological changes and mitochondria tGSH decrease in the kidneys, and consequently increased cisplatin‐nephrotoxic susceptibility. These data suggest the association of calorie intake with kidney susceptibility to cisplatin.
Long-Term Exposure to Source-Specific Fine Particles and Mortality—A Pooled Analysis of 14 European Cohorts within the ELAPSE Project

We assessed mortality risks associated with source-specific fine particles (PM2.5) in a pooled European cohort of 323,782 participants. Cox proportional hazard models were applied to estimate mortality hazard ratios (HRs) for source-specific PM2.5 identified through a source apportionment analysis. Exposure to 2010 annual average concentrations of source-specific PM2.5 components was assessed at baseline residential addresses. The source apportionment resulted in the identification of five sources: traffic, residual oil combustion, soil, biomass and agriculture, and industry. In single-source analysis, all identified sources were significantly positively associated with increased natural mortality risks. In multisource analysis, associations with all sources attenuated but remained statistically significant with traffic, oil, and biomass and agriculture. The highest association per interquartile increase was observed for the traffic component (HR: 1.06; 95% CI: 1.04 and 1.08 per 2.86 μg/m3 increase) across five identified sources. On a 1 μg/m3 basis, the residual oil-related PM2.5 had the strongest association (HR: 1.13; 95% CI: 1.05 and 1.22), which was substantially higher than that for generic PM2.5 mass, suggesting that past estimates using the generic PM2.5 exposure response function have underestimated the potential clean air health benefits of reducing fossil-fuel combustion. Source-specific associations with cause-specific mortality were in general consistent with findings of natural mortality.