Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Calcium and Signal Sensing Systems on Azorhizobium caulinodans Biofilm Formation and Host Colonization

Biofilm formation is important for establishing plants-microbe associations. The role of calcium on biofilm formation has been studied in many bacteria except rhizobia. In this study, we investigated the role of calcium for biofilm formation in Azorhizobium caulindans, which forms nodules in the stem and root of its host plant Sesbania rostrata. We found that calcium is essential for A. caulindans biofilm formation, in addition to the presence of extracellular matrix components, eDNA and proteins. Also, calcium-mediated biofilm formation was tested with chemotaxis, motility, cyclic di-GMP synthesis, and quorum sensing mutants. Finally, calcium was found to promote S. rostrata root colonization of A. caulinodans. In total, these results show that calcium is essential for A. caulindans biofilm formation, and it affects the interaction between A. caulinodans and host plant.
Face Forward: How Employees’ Digital Presence on Service Websites Affects Customer Perceptions of Website and Employee Service Quality

Confronted with increasing digitalization, service firms are challenged to sustain customer loyalty. A promising means to do so is to leverage the digital presence of service employees on their website. A large-scale field study and several experimental studies show that the digital presence of service employees on the firm website increases current website service quality perceptions and positively shapes memories related to employee service quality perceptions from past service encounters. Both effects indirectly increase customer loyalty and, in turn, financial performance, and are amplified by employee accessibility and a service firm’s customer orientation. The authors examine further boundary conditions for the memory process: only service employees evoke the beneficial spillover effect to employee service quality perceptions, and the spillover effect does not generalize to evaluations of product quality. Remarkably, an employee’s digital presence, although factually unrelated, augments customer perceptions of service employees’ competence and commitment and thus strengthens rather than erodes service employees’ role in customer–firm relationships. Theoretical and managerial implications deepen the understanding of how to add a human touch to digital channels.
Antimicrobial emulsifier – glycerol monolaurate impacts gut micobiome inducing distinct effects on metabolic syndrome in low-fat diet fed mice

Previous study demonstrated 150 mg·kg−1 glycerol monolaurate (GML) consumption induced gut microbiota dysbiosis and metabolic syndrome (MetS) in low-fat diet fed mice. However, little is known about the dose-effect of dietary GML modulating the gut microbiome alterations and its impacts on the induction of MetS in low-fat diet. Dietary GML-induced effects on MetS and gut microbiota alterations were investigated, combined with antibiotics-treated germ-free experiment and specific gut microbiota transplantation. Our results demonstrated that high-dose (500 mg·kg−1) GML alleviated MetS by significantly decreasing the body weight, weight gain, food intake, fat droplet size and percentage of abdominal fat, serum triglycerides (TG), LDL, LPS, TNF-α and atherogenic index, compared to the medium-dose (150 mg·kg−1) GML. Importantly, high-dose GML significantly increased Lactobacillus reuteri compared to the medium-dose GML. Co-occurrence network analysis revealed Lactobacillus reuteri was positively associated with the metabolic improvement of high-dose GML. Notably, antibiotics-treated germ-free experiment and Lactobacillus reuteri transplantation demonstrated that altered gut microbiota was necessary and sufficient for GML-induced distinct effects on metabolic syndrome. Our results indicate that GML impacts gut microbiome inducing distinct effects on metabolic syndrome, thereby calling for reassessing the safe dosage of GML and other non-specific antibacterial food additives. IMPORTANCE Growing evidence indicate that the broad use of food emulsifying agents may lead to increase the societal incidence of obesity/ MetS and other chronic inflammatory diseases. GML is widely and regularly consumed as a generally safe food emulsifier and as a potent antimicrobial agent in commonly foods such as meat products, cereals and soft beverage by the general public. Our results indicate that GML impacts gut microbiome inducing distinct effects on metabolic syndrome. Our study provides important and timely evidence supporting the emerging concept that non-specific antibacterial food additives have two-sided effect on gut microbiota contributing to the uncertainties for the incidence of obesity/metabolic syndrome and other chronic inflammatory diseases.
Association Between Vitamin D Status and Diabetic Complications in Patients With Type 2 Diabetes Mellitus: A Cross-Sectional Study in Hunan China

Background: Vitamin D status has been linked to diabetes-related complications due to multiple extraskeletal effects. We aimed to investigate the association between vitamin D deficiency (VDD) and diabetic vascular complications, including diabetic retinopathy (DR), diabetic kidney disease (DKD), and diabetic foot ulcers (DFU). Methods: A total of 4,284 Chinese patients with type 2 diabetic mellitus (T2DM) were enrolled into the cross-sectional study. VDD was defined as serum 25-hydroxyvitamin D <50 nmol/L. Demographic data, physical measurements, laboratory measurements, comorbidities, and related medications were collected and analyzed by VDD status. Poisson regression with robust variance estimation and binary logistic regression were performed to explore the relationship between VDD and diabetic complications. Results: The prevalence of VDD, DR, DKD, DFU accounted to 71.7% (95% confidence intervals [CI]: 70.3-73.0%), 28.5% (95% CI: 27.2-29.9%), 28.2% (95% CI: 26.8-29.5%), and 5.7% (95% CI: 5.1-6.5%), respectively. The prevalence ratios (95% CI) for DR and DKD by VDD status, adjusted for demographics, physical measurements, laboratory measurements, related complications, and comorbidities, and medications, were 1.093 (0.983-1.215) and 1.041 (0.937-1.156), respectively. The odds ratio (95% CI) for DFU by VDD status was 1.656 (1.159-2.367) in the final adjusted model. Meanwhile, the prevalence of VDD was significantly higher in patients with DFU compared with patients without DFU. Conclusions: The present study firstly indicated that VDD was significantly associated with a higher prevalence of DFU among Chinese T2DM patients. The association between VDD status and DR or DKD was not significant when adjusting for all potential covariates. Vitamin D screening or supplementation may be beneficial to prevent DFU and improve the prognosis of T2DM patients.
SARS-CoV-2 infection severity is linked to superior humoral immunity against the spike

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently causing a global pandemic. The antigen specificity and kinetics of the antibody response mounted against this novel virus are not understood in detail. Here, we report that subjects with a more severe SARS-CoV-2 infection exhibit a larger antibody response against the spike and nucleocapsid protein and epitope spreading to subdominant viral antigens, such as open reading frame 8 and non-structural proteins. Subjects with a greater antibody response mounted a larger memory B cell response against the spike, but not the nucleocapsid protein. Additionally, we revealed that antibodies against the spike are still capable of binding the D614G spike mutant and cross-react with the SARS-CoV-1 receptor binding domain. Together, this study reveals that subjects with a more severe SARS-CoV-2 infection exhibit a greater overall antibody response to the spike and nucleocapsid protein and a larger memory B cell response against the spike.
The Impact of an Intergenerational Dance Project on Older Adults’ Social and Emotional Well-Being

There has been strong interest in intergenerational arts practice in the United Kingdom since the 1980s; however, there is a generally weak evidence base for the effectiveness of intergenerational practice regardless of the domain. The aim of this study was to investigate the outcomes of an intergenerational arts project on participants’ social and psychological well-being using a mixed-methods, short-term longitudinal design. Generations Dancing brought together community artists with students (n = 25) and older adults (n = 11) living in Bedford. Over an 11-week period, participants worked together to produce a new dance performance and photography exhibition. Focus groups were conducted with the participants to explore their feelings about the collaboration across generations and communities. Participants also completed a battery of questionnaires preproject and postproject, to assess any change in their levels of well-being. Results indicate that the older adults showed increased confidence and willingness to connect with others; they got immense enjoyment from talking about their experience with others. Furthermore, the project helped to address negative stereotypes that the older adults had of working with the young people. The older adults enjoyed the students’ company and felt encouraged and supported by the young people. While a small number of challenges were identified, including difficulties in traveling to the workshops for vulnerable participants, most challenges were overcome through the older adults’ engagement in the project. For example, initial anxieties regarding the performance seemed insufficient to affect the participants’ overall enjoyment of the project. The findings were supported by the increased scores in relatedness, affect, and social well-being over time, but were not statistically significant. The results of this study indicate that intergenerational dance and arts projects can have wide-reaching positive impacts on both social and psychological well-being. However, there were a number of methodological challenges, including difficulty in recruiting sufficient numbers of both experimental and control groups for a robust quantitative evaluation of the data. These challenges highlight that “real life” settings and scenarios can influence the amount, nature, validity, and reliability of data collected. Going forward we encourage researchers to continue to consider innovative ways to address such methodological challenges.
Profiling FLT3 Mutations in Mexican Acute Myeloid Leukemia Pediatric Patients: Impact on Overall Survival

Background: Acute myeloid leukemia (AML) is the second most frequent leukemia in childhood. The FLT3 gene participates in hematopoietic stem cell proliferation. FLT3 mutations are recurrent in AML and influence prognosis. In Mexican pediatric AML patients, FLT3 mutational profile, and their clinical impact have not been evaluated. Aim of the study: This study aimed to identify the profile of FLT3 mutations in pediatric patients with de novo AML and to assess their possible influence on overall survival (OS) and other clinical features. Methods: Massive parallel target sequencing of FLT3 was performed in 80 patients. Results: FLT3 mutations [internal tandem duplication (ITD) or tyrosine kinase domain (TKD)] were identified in 24% of them. OS was significantly lower in FLT3POS cases than in FLT3NEG (p = 0.03). The average OS for FLT3POS was 1.2 vs. 2.2 years in FLT3NEG. There were no significant differences in the children's sex, age, percentage of blasts in bone marrow aspirate, or white blood cell count in peripheral blood at diagnosis between both groups. No differences were identified stratifying by the mutational load (high > 0.4) or type of mutation. The negative effect of FLT3 mutations was also observed in patients with acute promyelocytic leukemia (APL). Conclusions: FLT3 mutational profile is described in Mexican pediatric AML patients for the first time. Mutated FLT3 negatively impacts the outcome of AML patients, even considering the APL group. The clinical benefit from treatment with tyrosine kinase inhibitors in the FLT3POS pediatric patients needs to be assessed in clinical trials. FLT3 testing may contribute to better risk stratification in our pediatric AML patients.
Co-targeting myelin inhibitors and CSPGs enhances sensory axon regeneration within, but not into, the spinal cord

A major barrier to intraspinal regeneration after dorsal root (DR) injury is the DR entry zone (DREZ), the CNS/PNS interface. DR axons stop regenerating at the DREZ, even if regenerative capacity is increased by a conditioning lesion. This potent blockade has long been attributed to myelin-associated inhibitors and CSPGs, but incomplete lesions and conflicting reports have hampered conclusive agreement. Here we evaluated DR regeneration in adult mice, using novel strategies to facilitate complete lesions and comprehensive analyses, selective tracing of proprio-/mechanoreceptive axons with AAV2, and genetic or viral targeting of Nogo, MAG, OMgp, CSPGs and GDNF. Simultaneously eliminating Nogo/MAG/OMgp elicited little intraspinal penetration of DR axons, even with additional removal of CSPGs and a conditioning lesion. Their absence, however, synergistically enhanced GDNF-elicited intraspinal regeneration. We conclude that myelin inhibitors and CSPGs constrain intraspinal regrowth of DR axons, but that they are not the primary mechanism(s) stopping axons at the DREZ.
Space-Time Correlation for Three-Dimensional MIMO Channel Model Using Leaky Coaxial Cables in Rectangular Tunnel

The rapid development of high-speed train and Metro communications has provided new challenges for the application of MIMO technologies. Therefore, we propose a three-dimensional (3D) multiple-input multiple-output (MIMO) channel model using leaky coaxial cable (LCX) in a rectangular tunnel. The channel model is based on geometry-based single-bounce (GBSB) channel model and the electric field distribution of LCX in the tunnel environment. The theoretical expressions of channel impulse response (CIR) and space-time correlation function (CF) are also derived and analyzed. The CFs for different model parameters (moving velocity and moving time) and different regions of the tunnel are simulated by Monte Carlo method to verify the theoretical derivation at 1.8 GHz. In the same parametric configuration of nonstationary tunnel scenarios, the time delay of the first minimum value of CFs for LCX-MIMO is 1/5 of the time delay of the minimum value of CFs for dipole antennas MIMO when the train moving velocity is 360 km/h. It is shown that, for MIMO system, the performance of using LCXs is better than using dipole antennas.
The Liquid Maldistribution Analysis of the Trickle Bed Reactor with the CFD Method

The liquid phase maldistribution factor has been investigated in trickle bed reactor, and the results are compared with the previous measurement data from literature by using the Electrical Resistance Tomography. The simulation results are in agreement with the experimental results to some degree. The flow rates and particle sizes have been simulated with the method of multiphase flow. There are two different particles with average diameters of 3.4 mm and 5.3 mm. The flow rate has been studied ranging from 100 ml/min to 1100 ml/min. It has been found that the changes of the particles and liquid flow rates have a significant impact on the distribution of the liquid volume fraction. The internal liquid holdup is more serious, and the wall-flow phenomenon is more obvious in a bigger flow rate. The prediction of the liquid volume fraction distribution is a key research technique. Regression predictions have also been researched on the section near outlet, which can predict the internal flow state of the trickle bed under the condition of high temperature and high pressure. The average liquid volume fraction is linear with flow rates. The maldistribution factor is the index correlation with the flow rates. The results and main conclusions can be used to predict the distributions and get the properties in a trickle bed reactor.
Adult cancer patients’ barriers toward pain management: A literature review

Background and objective: Cancer pain is the most common symptom among cancer patients. Despite strategies to control cancer pain, cancer patients’ beliefs and attitudes influence the effectiveness of cancer pain management. The aim of this literature review was to identify and explore adult cancer patients’ barriers toward pain management. Methods: A literature review was conducted. CINAHL, Medline, and PsychINFO databases were searched for relevant articles from 2008 to 2019. Twenty one articles were included in this literature review. Thematic analysis was conducted to identify and explore adult cancer patients’ barriers toward pain management. Results: This literature review revealed several patient barriers toward pain management. These barriers were categorized into cognitive barriers that include poor pain communication, fatalism, and fear of addiction and tolerance; sensory barrier, such as fear of drug side effects; affective barriers, such as anxiety and depression, and socio-demographic barriers that influence cancer pain management. Conclusions: Adult cancer patients’ barriers toward pain management significantly compromise the effectiveness of pain management and affect cancer patients’ quality of life. A better understanding of cancer patients’ barriers toward pain management by the healthcare providers will result in better assessment and management of these barriers and will enhance evidenced-based patient education.
Comparative Histological Study on the Oviduct of Developing and Laying Hens (Gallus domesticus)

Comparative histological study on the oviduct of developing and laying hens revealed pseudostratified ciliated columnar epithelium in entire portion of oviduct except funnel part of the infundibulum, which was simple columnar. The mucosal folds of the developing hens differed from those of laying hens. Lamina propria was comparatively thicker in laying hens than developing hens. The tubular glands of laying hens were more developed but less in number than developing hens. In most of the regions there was clear demarcation of inner circular and outer longitudinal muscle fibres. These muscles fibres were spirally oriented which were interlacing each other in between the bundles of circular muscle fibres. The serosa was comparatively thicker in laying hen.
Estimate of correlation between the meteorological drought in Ethiopia and the hydrological drought in Egypt

Drought is one of the most complex natural phenomena and one of the main natural causes affecting agriculture, the economy and the environment in the world, and its impact has become evident significantly on the level of life on the globe in recent decades. It leads to droughts in different regions, so assessing the intensity of the drought period is one of the most important tools for sustainable agriculture, as drought has become a phenomenon in many parts of the world, especially in the East and North Africa region.The most important type of drought is metrological and hydrological drought, Drought indices were used to survey drought and demonstrate its performance. As a country in the world, Egypt suffers from water scarcity, especially in recent years. Therefore, the importance of studying Ethiopia as a study area comes due to the presence of the Blue Nile, which contributes about 85% of the Nile River's revenue.Metrological drought was calculated by the Standard Precipitation Index (SPI), and by hydrological by the Streamflow Drought Index (SDI) during the period from 1950 to 2017 based on the availability of the recorded data for meteorological stations in Ethiopia, and the streamflow for Dongola station, and then these data were evaluated using various methods, including homogeneity between the data for each station by considering the different time scales of periods 1, 3, 6, 9, and 12 months. , As a result, There are three seasons of rain in Ethiopia, Kiremt is the main rainy season from June to September, Bega is the dry season from October to January, Belg is the lowest rainy season from February to May and Ethiopia characterized by four precipitation regimes, and we found a correlation between the meteorological index (SPI) for five stations in Ethiopia, and the hydrological index (SDI) for Dongola.Keywords: Ethiopia; Dongola; Meteorological drought; Hydrological drought.
The clinical, histologic, and genotypic spectrum of SEPN1-related myopathy

Objective To clarify the prevalence, long-term natural history, and severity determinants of SEPN1-related myopathy (SEPN1-RM), we analyzed a large international case series. Methods Retrospective clinical, histologic, and genetic analysis of 132 pediatric and adult patients (2–58 years) followed up for several decades. Results The clinical phenotype was marked by severe axial muscle weakness, spinal rigidity, and scoliosis (86.1%, from 8.9 ± 4 years), with relatively preserved limb strength and previously unreported ophthalmoparesis in severe cases. All patients developed respiratory failure (from 10.1±6 years), 81.7% requiring ventilation while ambulant. Histopathologically, 79 muscle biopsies showed large variability, partly determined by site of biopsy and age. Multi-minicores were the most common lesion (59.5%), often associated with mild dystrophic features and occasionally with eosinophilic inclusions. Identification of 65 SEPN1 mutations, including 32 novel ones and the first pathogenic copy number variation, unveiled exon 1 as the main mutational hotspot and revealed the first genotype–phenotype correlations, bi-allelic null mutations being significantly associated with disease severity (p = 0.017). SEPN1-RM was more severe and progressive than previously thought, leading to loss of ambulation in 10% of cases, systematic functional decline from the end of the third decade, and reduced lifespan even in mild cases. The main prognosis determinants were scoliosis/respiratory management, SEPN1 mutations, and body mass abnormalities, which correlated with disease severity. We propose a set of severity criteria, provide quantitative data for outcome identification, and establish a need for age stratification. Conclusion Our results inform clinical practice, improving diagnosis and management, and represent a major breakthrough for clinical trial readiness in this not so rare disease.
Comparing the mortality of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) caused by entomopathogenic bacteria and Serratia marcescens (Enterobacteriales: Enterobacteriaceae)

To investigate whether Serratia marcescens (Enterobacteriales: Enterobacteriaceae) isolated from Protaetia brevitarsis seulensis(Coleoptera: Cetoniidae) acts as an opportunistic bacterium in peroral infection, the primary entomopathogenic bacteria Bacillus thuringiensis (Bacillales: Bacillaceae) and Paenibacillus popilliae (Eubacteriales: Bacillaceae) were added to sawdust to perform a bioassay experiment. We found that peroral infection caused by S. marcescens could be fatal beyond a concentration of 4 × 10 8 pfu/mL in 2 nd stage P. b. seulensis larvae