Miracle-dz/UniFAR-FTUs huggingface dataset:
Modulation of certainty and valence in cardiac autonomic regulation mediated by high depressive risk

Hui-Ling Chan, Maro G. Machizawa, Ryohei Mizuochi, & Shigeto Yamawaki 1 Center for Brain, Mind and KANSEI Sciences Research, Hiroshima University, Hiroshima 7348551, Japan. Tel: +81–82–257–1722. E-mail: chanhl@hiroshima-u.ac. jp 2 Center for Brain, Mind and KANSEI Sciences Research, Hiroshima University, Tokyo 130-0003, Japan. Tel: +81–50–8880–8806. E-mail: machizawa@hiroshima-u.ac. jp 3 Center for Brain, Mind and KANSEI Sciences Research, Hiroshima University, Hiroshima 7348551, Japan. Tel: +81–82–257–1722. E-mail: d145756@hiroshima-u.ac. jp 4 Center for Brain, Mind and KANSEI Sciences Research, Hiroshima University, Hiroshima 7348551, Japan. Tel: +81–82–257–1722. E-mail: yamawaki@hiroshima-u.ac. jp * The two authors contributed to the manuscript equally.
Integrin-Driven Axon Regeneration in the Spinal Cord Activates a Distinctive CNS Regeneration Program

The peripheral branch of sensory dorsal root ganglion (DRG) neurons regenerates readily after injury unlike their central branch in the spinal cord. However, extensive regeneration and reconnection of sensory axons in the spinal cord can be driven by the expression of α9 integrin and its activator kindlin-1 (α9k1), which enable axons to interact with tenascin-C. To elucidate the mechanisms and downstream pathways affected by activated integrin expression and central regeneration, we conducted transcriptomic analyses of adult male rat DRG sensory neurons transduced with α9k1, and controls, with and without axotomy of the central branch. Expression of α9k1 without the central axotomy led to upregulation of a known PNS regeneration program, including many genes associated with peripheral nerve regeneration. Coupling α9k1 treatment with dorsal root axotomy led to extensive central axonal regeneration. In addition to the program upregulated by α9k1 expression, regeneration in the spinal cord led to expression of a distinctive CNS regeneration program, including genes associated with ubiquitination, autophagy, endoplasmic reticulum (ER), trafficking, and signaling. Pharmacological inhibition of these processes blocked the regeneration of axons from DRGs and human iPSC-derived sensory neurons, validating their causal contributions to sensory regeneration. This CNS regeneration-associated program showed little correlation with either embryonic development or PNS regeneration programs. Potential transcriptional drivers of this CNS program coupled to regeneration include Mef2a, Runx3, E2f4, and Yy1. Signaling from integrins primes sensory neurons for regeneration, but their axon growth in the CNS is associated with an additional distinctive program that differs from that involved in PNS regeneration. SIGNIFICANCE STATEMENT Restoration of neurologic function after spinal cord injury has yet to be achieved in human patients. To accomplish this, severed nerve fibers must be made to regenerate. Reconstruction of nerve pathways has not been possible, but recently, a method for stimulating long-distance axon regeneration of sensory fibers in rodents has been developed. This research uses profiling of messenger RNAs in the regenerating sensory neurons to discover which mechanisms are activated. This study shows that the regenerating neurons initiate a novel CNS regeneration program which includes molecular transport, autophagy, ubiquitination, and modulation of the endoplasmic reticulum (ER). The study identifies mechanisms that neurons need to activate to regenerate their nerve fibers.
Among-individual variation of risk-taking behaviour in group and solitary context is uncorrelated but independently repeatable in a juvenile Arctic charr (Salvelinus alpinus) aquaculture strain

Behavioural traits have been shown to have implications in fish welfare and growth performances in aquaculture. If several studies have demonstrated the existence of repeatable and heritable behavioural traits (i.e., animal personality), the methodology to assess personality in fishes is often carried out in solitary context, which appears to somewhat limit their use from a selective breeding perspective because these tests are too time consuming. To address this drawback, group-based tests have been developed. In Nordic country, Arctic charr (Salvelinus alpinus) is widely used in aquaculture, but no selection effort on behavioural traits has yet been carried out. Specifically, in this study we examined if risk-taking behaviour was repeatable and correlated in group and solitary context and if the early influences of physical environment affect the among-individual variation of behavioural trait across time in order to verify whether a group risk-taking test could be used as a selective breeding tool. Here, we found that in both contexts and treatments, the risk-taking behaviour was repeatable across a short period of 6 days. However, no cross-context consistency was found between group and solitary, which indicates that Arctic charr express different behavioural trait in group and solitary.
Impact of Molecular Modifications on the Immunogenicity and Efficacy of Recombinant Raccoon Poxvirus-Vectored Rabies Vaccine Candidates in Mice

Rabies is an ancient disease that is responsible for approximately 59,000 human deaths annually. Bats (Order Chiroptera) are thought to be the original hosts of rabies virus (RABV) and currently account for most rabies cases in wildlife in the Americas. Vaccination is being used to manage rabies in other wildlife reservoirs like fox and raccoon, but no rabies vaccine is available for bats. We previously developed a recombinant raccoonpox virus (RCN) vaccine candidate expressing a mosaic glycoprotein (MoG) gene that protected mice and big brown bats when challenged with RABV. In this study, we developed two new recombinant RCN candidates expressing MoG (RCN-tPA-MoG and RCN-SS-TD-MoG) with the aim of improving RCN-MoG. We assessed and compared in vitro expression, in vivo immunogenicity, and protective efficacy in vaccinated mice challenged intracerebrally with RABV. All three candidates induced significant humoral immune responses, and inoculation with RCN-tPA-MoG or RCN-MoG significantly increased survival after RABV challenge. These results demonstrate the importance of considering molecular elements in the design of vaccines, and that vaccination with either RCN-tPA-MoG or RCN-MoG confers adequate protection from rabies infection, and either may be a sufficient vaccine candidate for bats in future work.
Factors affecting social sustainability in the historical city centres of Iran

ABSTRACT The current study’s primary purpose is to explore the critical social variables that could affect the urban regeneration of historical city centres in Iran. The study was based on a questionnaire-based survey in four historical city centres: Kashan, Naeen, Ardakan and Yazd. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were used to analyse the collected data. SPSS software was applied to extract the factors, and the Analysis of Moment Structures software (AMOS 24) used to confirm these factors through a structural model. Based on the results, seven significant factors proved most relevant: security/safety; accessibility; equity; participation; quality of life; solidarity and income. The highest correlations between them were observed in security and participation, accessibility and quality of life, and accessibility and equity. These relationships should guide urban planners and policy-makers dealing with historical city centres.
Contrasts in Top Soil Infiltration Processes for Degraded vs. Restored Lands. A Case Study at the Perijá Range in Colombia

Governments are increasingly committing to significant ecological restoration. However, the impacts of forest restoration on local hydrological services are surprisingly poorly understood. Particularly, limited information is available about the impacts of tree planting on soil infiltration processes and runoff pathways. Thus, we investigated the saturated hydraulic conductivity (Ks) and preferential flow pathways in three land-cover types: (i) Active Restoration, (ii) Degraded Land, and (iii) Reference Forest, with contrasting differences in soil profile and land use history in the municipality of La Jagua de Ibirico, César department, Colombia. We conducted soil sampling, using the Beerkan method to determine Ks values. We also measured vegetation attributes (i.e., canopy cover, vegetation height, diameter at breast height, and total number of trees) and carried out three dye tracer experiments for each study site. The blue dye experiments revealed that near surface matrix infiltration was dominant for Degraded Land, while at the Active Restoration and Reference Forest, this only occurred at local surface depressions. The general infiltration pattern at the three land uses is indicated as being macropore flow with mixed interaction with the matrix and highly affected by the presence of rock fragments. The deeper infiltration patterns occur by preferential flow due to the presence of roots and rock fragments. The mean Ks for the Active Restoration (240 mm h−1) was much higher than the Ks at Degraded Land (40 mm h−1) but still considerably lower than the Reference Forest (324 mm h−1). These results indicate that top soil infiltration capacity and soil physical parameters not only directly regulate the amount of infiltration but also infiltration patterns and runoff processes, leading to lower infiltration and increased excess overland flow for Degraded Land than for other land uses.
Lower Levels of Vestibular Developmental Stability in Slow-Moving than Fast-Moving Primates

The vestibular system of the mammalian inner ear senses angular and linear velocity of the head and enables animals to maintain their balance. Vestibular anatomy has been studied extensively in order to link its structure to particular kinds of locomotion. Available evidence indicates that, in primates, slow-moving species show higher levels of vestibular variation than fast-moving taxa. We analysed intraspecific morphological variation and fluctuating asymmetry (FA) levels in the semicircular canal systems of six species of lorisiform primates: three slow-moving lorisids and three fast-moving galagids. Our results showed clear differences in levels of intraspecific variation between slow-moving and fast-moving taxa. Higher levels of variation were responsible for deviations from coplanarity for synergistic pairs of canals in slower taxa. Lorisids also presented higher levels of FA than galagids. FA is a better indicator of agility than intraspecific variation. These results suggest that in order to function efficiently in fast taxa, semicircular canal systems must develop as symmetrically as possible, and should minimise the deviation from coplanarity for synergistic pairs. Higher levels of variation and asymmetry in slow-moving taxa may be related to lower levels of stabilising selection on the vestibular system, linked to a lower demand for rapid postural changes.
656 Cough, a rare and not well recognized symptom of lead perforation

Along with relevant progress in technology, pacemaker implantation is continuously improving its safety and efficacy in treating patients with bradyarrhythmias. Despite this, this procedure has several complications, including haematoma, pneumothorax, lead dislodgement, infection, lead perforation, and tamponade. A 64-year-old woman underwent loop recorder implantation, after recurrent loss of consciousness, in order to assess arrhythmic causes of syncope. Two weeks later, an episode of paroxysmal complete AV block, conditioning a pause of 3 s, was recorded. Thus, the patient was scheduled for urgent dual-chamber pacemaker implantation. No complication apparently occurred during the procedure. An active fixation ventricular lead was positioned in right ventricular septal apex while passive fixation atrium lead in the right appendage. Soon after implantation the patient started to suffer by non-productive cough, clearly related to ventricular stimulation, either in DDD or in VVI pacing modality. During spontaneous ventricular activation (RBBB) no symptoms occurred. Transthoracic echocardiography, performed the day after implantation, revealed a small pericardial effusion (diastolic diameter < 10 mm) along the apical segments, near the tip of the right ventricular lead. Suspicion of right ventricular lead perforation arised. The patient underwent urgent contrast chest CT confirming pericardial effusion, and showing an intramyocardium placement of the right ventricular apical lead. No active bleeding in pericardium was observed. Due to persistence of symptoms, we decided to perform right ventricular lead repositioning in right middle septum, with pericardiocentesis back-up promptly available. Post-procedure, palpitation, and cough abruptly disappeared. After 3 months follow-up, no significant symptoms were reported and pericardial effusion gradually disappeared. We describe a singular case of cough, as atypical symptom immediately after pacemaker implantation. Pericardial effusion and contrast-CT showing intra-myocardial position of the tip guided our suspicion to a possible right ventricular lead microperforation. Although right ventricular lead parameters were completely normal this findings didn’t exclude RV perforation. The lead perforation is known as a rare complication of device implantation. Typical symptoms of RV lead perforation are chest pain and hypotension. The patient described in our case showed a haemodynamically stable pericardial effusion accompanied by non-productive cough, clearly time-related to RV stimulation. In literature, there is only another similar case report. The cough is a rare and not well recognized symptom of lead perforation. Early diagnosis of RV perforation allows to perform urgently and safely (pericardiocentesis back-up) lead replacement/repositioning. Echocardiography and contrast-CT could be useful in order to assess a possible pericardial effusion or intramyocardial/pericardial position of RV lead tip.
Does Mobility Increase the Productivity of Inventors? New Evidence from a Quasi-Experimental Design

Although labor mobility has been recognized as a key mechanism to transfer tacit knowledge, prior research on inventors has so far neglected to address the question of the consequence of a move on inventive performance. This paper improves on the current R&D literature by presenting a quasi-experimental approach to explore the effect of a specific move of an inventor on his performance. The quasi-experiment provides a favorable setting to test this relationship since it allows interpreting changes of inventive performance causal to a particular move. Results reveal that in the post-move period inventors produce more patentable innovations that are characterized by a higher grant rate and by higher value. However, the gains from movement seem to dissipate over time. Data for the analysis was derived from a survey of German inventors (N = 3,049).
The Use of Rhetorical Strategies in Argumentative Essays

The most challenging skill perceived by students when they learn the English language is the writing skill. This recent study would like to identify the rhetorical strategies used by good writers and poor writers. Two participants were selected, and written essays was the instrument employed for this study. Both participants were required to write an essay on ‘Should examinations be abolished? ’ The essays written were analysed using a coding technique. The findings indicated that both writers utilised the three elements, Logos, Ethos and Pathos, differently. Both were considerate to the readers when they wrote the essays and presented their message, which was also heavily emphasised. However, they did not focus on their roles as writers. Based on the findings, it can be concluded that teachers need to help students familiarise themselves with rhetorical strategies. As for students, they should be aware of the rhetorical strategies to enhance their writing skills to write argumentative essays.
Evidence for Health-Promoting Properties of Lepidium sativum L.: An Updated Comprehensive Review.

Lepidium sativum L. is a common herb distributed worldwide, used as a food ingredient and therapeutic agent in traditional medicine for treating health-related disorders. L. sativum and its extracts have been described to possess numerous biological activities including antimicrobial, antidiabetic, antioxidant, antidiarrheal, anticancer, and numerous health-promoting effects in in vivo and in vitro studies. The purpose of this review is to summarize the findings describing important biological functions and therapeutic effects of L. sativum in various cell lines and animal models. In this review, the English-language articles were gathered from electronic databases including Web of Science, PubMed and Google Scholar with no time limit applied to any database. The search terms used in this review include, "Lepidium sativum L. " and/or "chemical composition", "health benefits", "antimicrobial", "antioxidant", "anticancer", "diuretic", "nephro-protection", "antidiarrheal", "antidiabetic", "anti-asthmatic", "neuroprotection", "metabolic", "bone fracture", and "reproductive performance". Additional and eligible studies were collected from reference lists of appropriate articles. The information presented will be helpful to attract more interest toward medicinal plants by defining and developing novel clinical applications and new drug formulations in the future. Pre-clinical studies showed that L. sativum possesses potent health-promoting effects involving various molecular mechanisms. Taken all together, data suggested that identified herbal plants such as L. sativum, can be exploited as nutritional and therapeutic agents to combat various ailments. Despite much research in this field, further comprehensive in vitro/in vivo studies and clinical trials are needed to identify the mechanisms underlying the biological and therapeutic activities of L. sativum.
A methodology to integrate site characterization information into groundwater‐driven health risk assessment

A methodology is presented that incorporates site characterization information (in the form of hydraulic conductivity measurements) into a health risk assessment to individuals utilizing contaminated household water developed from groundwater sources. This methodology explicitly incorporates uncertainty (including the statistical parameter uncertainty arising from a limited number of hydraulic conductivity measurements) and variability (such as the variability in individual physiology) in all parameters, resulting in a nested Monte Carlo procedure. The methodology is demonstrated using a hypothetical case study where the sensitivity of various end points (including human health risk) to increased sampling of hydraulic conductivity are explored. It is shown that an increase in the number of measurements of hydraulic conductivity decreases the error in and uncertainty of predicted human health risk. Cost‐benefit curves are developed and incorporated into a simple decision framework to determine the number of samples of hydraulic conductivity that are needed to accurately predict the human health risk from a contaminated site.
Autophagy inhibition mediated by MCOLN1/TRPML1 suppresses cancer metastasis via regulating a ROS-driven TP53/p53 pathway

ABSTRACT Compelling evidence has demonstrated that macroautophagy/autophagy plays an important role in regulating multiple steps of metastatic cascades; however, the precise role of autophagy in metastasis remains unclear. This study demonstrates that autophagy inhibition induced by MCOLN1/TRPML1 suppresses cancer metastasis by evoking the ROS-mediated TP53/p53 pathway. First, we found that MCOLN1-mediated autophagy inhibition not only profoundly inhibits both migration and invasion in malignant melanoma and glioma cell lines in vitro, but also suppresses melanoma metastasis in vivo. Second, our study reveals that autophagy inhibition induced by MCOLN1 leads to damaged mitochondria accumulation followed by large quantities of ROS release. Third, we demonstrate that the elevated ROS resulting from autophagy inhibition subsequently triggers TP53 activity, which in turn modulates expression of its downstream targets that are involved in a broad spectrum of the metastatic cascade to suppress metastasis including MMP members and TWIST. In summary, our findings have established a mechanism by which autophagy inhibition suppresses metastasis via the ROS-TP53 signaling pathway. More importantly, our study demonstrates that autophagy inhibition through stimulation of MCOLN1 could evidently be one of the therapeutic potentials for combating cancer metastasis. Abbreviations: 3-MA: 3-methyladenine; AA: amino acid; ATG5: autophagy related 5; ATG12: autophagy-related 12; Baf-A1: bafilomycin A1; CCCP: carbonyl cyanide m-chlorophenylhydrazone; CQ: chloroquine; DMEM: Dulbecco’s Modified Eagle Medium; EMT: epithelial–mesenchymal transition; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HEK: human embryonic kidney; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MCOLN1/TRPML1: mucolipin TRP cation channel 1; MMP: matrix metallopeptidase; NC: negative control; NRK: normal rat kidney; PBS: phosphate-buffered saline; shRNA: short hairpin RNA; siRNA: short interfering RNA; SQSTM1/p62: sequestosome 1; ULK1: unc-51 like autophagy-activating kinase 1.
Finite Element Simulation of FRP Strengthened Reinforced Concrete Slabs under Two Independent Air Blasts

Multiple detonations might occur in both accidental explosions and terrorism attacks. Generally, normal reinforced concrete (RC) structures which are not designed to withstand high intensity blast loads are not capable of withstanding explosions from a single blast let alone a sequence of more than one blast. Since concrete is often highly cracked and damaged from the first blast, the remaining deteriorated concrete and steel reinforcement in a RC member becomes very vulnerable to collapse. This paper reports on the feasibility of using fibre reinforced polymer (FRP) to strengthen a normal RC slab capable of sustaining two independent air blasts. Apart from the experimental investigation, numerical studies have been conducted to verify the concrete and FRP material models when they are utilized to predict the behaviour of FRP-RC structures under multiple blasts. This article provides guidance on how to choose appropriately between the two existing concrete models available in the LS-DYNA code.
A methodology to boost data-driven decision-making process for a modern maintenance practice

Abstract Maintenance is evolving due to the double-sided influence of the Asset Management paradigm and digitalization. In this evolution, assessing the maintenance management process status in terms of process completeness, information and data completeness and integration is paramount to boost reliable data-driven decision-making. Grounding on Design Science Research, a methodology is realized to favour the comparison of two data models, a reference one and a company-specific one, used as a means to evaluate the process status. In particular, the methodology embeds a reference data model for the maintenance management process. Both methodology and data model are artefacts tested and refined during action research in an automotive company willing to improve the maintenance management process. The application of both artefacts demonstrates that the company is facilitated in planning improvement actions for various time horizons to foster a modern maintenance practice whose decision-making is more data-driven.