Miracle-dz/UniFAR-FTUs huggingface dataset:
Vegetation Change and Its Relationship with Climate Factors and Elevation on the Tibetan Plateau

As the “roof of the world”, the Tibetan Plateau (TP) is a unique geographical unit on Earth. In recent years, vegetation has gradually become a key factor reflecting the ecosystem since it is sensitive to ecological changes especially in arid and semi-arid areas. Based on the normalized difference vegetation index (NDVI) dataset of TP from 2000 to 2015, this study analyzed the characteristics of vegetation variation and the correlation between vegetation change and climatic factors at different time scales, based on a Mann–Kendall trend analyses, the Hurst exponent, and the Pettitt change-point test. The results showed that the vegetation fractional coverage (VFC) generally increased in the past 16 years, with 60.3% of the TP experiencing an increase, of which significant (p < 0.05) increases accounted for 28.79% and were mainly distributed in the north of the TP. Temperature had the largest response with the VFC on the seasonal scale. During the growing season, the correlation between precipitation and sunshine duration with VFC was high (p < 0.05). The change-points of the VFC were mainly distributed in the north of the TP during 2007–2009. Slope and elevation had an impact on the VFC; the areas with large vegetation change are mainly distributed in slopes <20° and elevation of 3000–5000 m. For elevation above 3000–4000 m, the response of the VFC to precipitation and temperature was the strongest. This study provided important information for ecological environment protection and ecosystem degradation on the Tibetan Plateau.
Lipidomic Profiling Reveals Significant Perturbations of Intracellular Lipid Homeostasis in Enterovirus-Infected Cells

Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the most common causes of hand, foot, and mouth disease. Severe EV-A71 and CV-A16 infections may be associated with life-threatening complications. However, the pathogenic mechanisms underlying these severe clinical and pathological features remain incompletely understood. Lipids are known to play critical roles in multiple stages of the virus replication cycle. The specific lipid profile induced upon virus infection is required for optimal virus replication. The perturbations in the host cell lipidomic profiles upon enterovirus infection have not been fully characterized. To this end, we performed ultra-high performance liquid chromatography–electrospray ionization–quadrupole–time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS)-based lipidomics to characterize the change in host lipidome upon EV-A71 and CV-A16 infections. Our results revealed that 47 lipids within 11 lipid classes were significantly perturbed after EV-A71 and CV-A16 infection. Four polyunsaturated fatty acids (PUFAs), namely, arachidonic acid (AA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA), and eicosapentaenoic acid (EPA), were consistently upregulated upon EV-A71 and CV-A16 infection. Importantly, exogenously supplying three of these four PUFAs, including AA, DHA, and EPA, in cell cultures significantly reduced EV-A71 and CV-A16 replication. Taken together, our results suggested that enteroviruses might specifically modulate the host lipid pathways for optimal virus replication. Excessive exogenous addition of lipids that disrupted this delicate homeostatic state could prevent efficient viral replication. Precise manipulation of the host lipid profile might be a potential host-targeting antiviral strategy for enterovirus infection.
Assessing the Correlations between Different Traits in Copper-Sensitive and Copper-Resistant Varieties of Jute (Corchorus capsularis L.)

The current study was conducted to explore the potential for phytoremediation in different varieties of jute grown under toxic concentrations of copper (Cu). For this purpose, a Petri dish experiment was conducted under controlled conditions using four varieties of jute, i.e., HongTieGuXuan, C-3, GuBaChangaJia, and ShangHuoMa, grown in double filter paper under 50 µmol L−1 of artificially spiked copper (Cu) using CuSO4.H2O. The results of the present study revealed that jute varieties C-3 and HongTieGuXuan were able to survive under high concentrations of Cu without a significant decrease in plant height, plant fresh and dry weights, total chlorophyll content, or seed germination, while varieties GuBaChangaJia and ShangHuoMa exhibited a significant reduction in their growth and biomass. Furthermore, high concentrations of Cu in the medium resulted in lipid peroxidation. This could be due to the oxidative damage induced in the roots and leaves of the jute varieties, which might be a result of by hydrogen peroxide (H2O2) and electrolyte leakage. Reactive oxygen species (ROS) generated due to Cu toxicity can be overcome by the increasing activity of antioxidants, and it was also noted that GuBaChangaJia and ShangHuoMa exhibited high Cu stress, while C-3 and HongTieGuXuan showed some resistance to Cu toxicity. Contrastingly, Cu accumulation and uptake was higher in C-3 and HongTieGuXuan, while a little Cu was accumulated in the roots and leaves of GuBaChangaJia and ShangHuoMa. On the basis of these findings, it can be suggested that C-3 and HongTieGuXuan have the potential to cope with Cu stress and can be considered Cu-resistant varieties, while GuBaChangaJia and ShangHuoMa are considered Cu-sensitive varieties. Moreover, C-3 and HongTieGuXuan have the potential to revoke large amounts of Cu, and can be cultivated as phytoremediation tools in Cu-contaminated soil.
Prevalence and pattern of consanguineous marriage among educated married individuals in Riyadh

Abstract Consanguineous marriage is preferred in many countries, especially by Muslims. Despite the increasing education rate in Saudi Arabia, the prevalence of consanguineous marriage does not seem to be decreasing as quickly as expected. The present study aimed to investigate the current prevalence of consanguineous marriage among educated married adults in Riyadh and to determine the factors favouring it. The cross-sectional study was conducted in 2017–18 using an online questionnaire. A total of 550 questionnaires were sent to married adults of both sexes and 417 responded, giving a response rate of 75.8%. The questionnaire consisted of two parts: the first section asked for demographic data such as age, sex, educational level, residential area and family size. The second part was about consanguineous marriage and its degree if present, family history of consanguineous marriage and level of awareness of its potential negative impact on offspring. It was found that the prevalence of consanguineous marriage among the participating educated adults was 39.8% and most of these were married to a first cousin. Neither level of education nor age affected the likelihood of consanguineous marriage, but predictors for the practice among the educated participating adults were having a family history of consanguineous marriage, having consanguineous parents and having a personal preference for consanguineous marriage. In conclusion, the prevalence of consanguineous marriage among educated adults in Riyadh was still high in 2018, especially among first-cousin relatives, and this was related to family history and personal preference rather than educational level or age. It is recommended that further research is conducted to assess the level of knowledge about, and attitude towards, consanguineous marriage among adults in Saudi Arabia.
Effect of Obesity and Other Risk Factors on Hypertension among Women of Reproductive Age in Ghana: An Instrumental Variable Probit Model

Background: The growing incidence of mortality as a result cardiovascular diseases (CVDs) is a major public health concern in several developing countries. In Ghana, unhealthy food consumption pattern and sedentary lifestyle are promoting overweight and obesity, with significant consequences on the incidence of CVDs. Specifically, hypertension morbidity is now a public health concern among Ghanaian health policy makers. This paper analysed the effect of body mass index (BMI)/arm circumference and other associated factors on hypertension risk among women of reproductive ages in Ghana. Methods: The data were collected as Demographic and Health Survey (DHS) in 2014. This paper analysed the subset of the data that were collected from eligible women 15–49 years of age. The total sample was 9396, while 9367 gave consents to have their blood pressure measured. Data were analysed with instrumental probit regression model with consideration of potential endogeneity of BMI and arm circumference. Results: The results showed that 25% of the women were either overweight or obese, while 13.28% were hypertensive. Women from the Greater Accra (18.15%), Ashanti (15.53%) and Volta (15.02%) regions had the highest incidences of hypertension. BMI and arm circumferences were truly endogenous and positively associated with the probability of being hypertensive. Other factors that influenced hypertension were age of women, region of residence, urban/rural residence, being pregnant, access to medical insurance, currently working, consumption of broth cubes, processed can meats, salted meat and fruits. Conclusion: It was concluded that hypertension risk was positively associated with being overweight, obesity, age and consumption of salted meat.It was inter aliaemphasized that engagement in healthy eating with less consumption of salted meats, and more consumption of fruits would assist in controlling hypertension among Ghanaian women.
A Global Model for the Cardiovascular and Respiratory System

In this thesis a global model for the cardiovascular and respiratory system is developed. The cardiovascular part of the model is based on the four compartment model by Grodins and includes modifications by Kappel and Peer. The respiratory part is based on the two compartment model by Khoo et al. The underlying models are reviewed, extended and combined. The combined model contains as subsystems the systemic and pulmonary circulation, the left and right ventricles, and the tissue and the lung compartment. Mechanisms included are Frank-Starling’s law, the Bowditch effect, and variable cerebral blood flow. In particular, the model is adapted to the situation of dynamic exercise. The initial anaerobic energy supply, the mechanism of metabolic autoregulation in the peripheral regions, and the dilatation of pulmonary vessels are taken into account. Heart rate and alveolar ventilation are assumed to be the quantities through which the central nervous system controls the mean arterial blood pressure and the blood gas concentrations. The transition from rest to exercise under a constant ergometric workload is simulated. Here the action of cardiovascular and respiratory control is represented by a linear feedback control which minimizes a quadratic cost functional. As an alternative, also a nonlinear feedback is implemented.
“A Step and a Ceiling”: mechanical properties of Ca2+ spark vasoregulation in resistance arteries by pressure‐induced oxidative activation of PKG

We investigated the biomechanical relationship between intraluminal pressure within small mesenteric resistance arteries, oxidant activation of PKG, Ca2+ sparks, and BK channel vasoregulation. Mesenteric resistance arteries from wild type (WT) and genetically modified mice with PKG resistance to oxidative activation were studied using wire and pressure myography. Ca2+ sparks and Ca2+ transients within vascular smooth muscle cells of intact arteries were characterized using high‐speed confocal microscopy of intact arteries. Arteries were studied under conditions of varying intraluminal pressure and oxidation. Intraluminal pressure specifically, rather than the generic stretch of the artery, was necessary to activate the oxidative pathway. We demonstrated a graded step activation profile for the generation of Ca2+ sparks and also a functional “ceiling” for this pressure –‐sensitive oxidative pathway. During steady state pressure ‐ induced constriction, any additional Ca2+ sensitive‐K+ channel functional availability was independent of oxidant activated PKG. There was an increase in the amplitude, but not the Area under the Curve (AUC) of the caffeine‐induced Ca2+ transient in pressurized arteries from mice with oxidant‐resistant PKG compared with wild type. Overall, we surmise that intraluminal pressure within resistance arteries controls Ca2+ spark vasoregulation through a tightly controlled pathway with a graded onset switch. The pathway, underpinned by oxidant activation of PKG, cannot be further boosted by additional pressure or oxidation once active. We propose that these restrictive characteristics of pressure‐induced Ca2+ spark vasoregulation confer stability for the artery in order to provide a constant flow independent of additional pressure fluctuations or exogenous oxidants.
Success of Two Caries Risk Assessment Tools in Children: A Pilot Study With a 3-Year Follow-Up

Caries risk is defined as the dental caries probability under the individual bacteriological environment, dietary habits, and environmental factors. Assessment of caries risk plays a major role in the prevention of dental caries. The aim of this study was to compare the effectiveness of two different caries risk assessment methods on caries-free preschool children. A sample of 90 caries-free 4- to 6-year-old children were selected from 400 preschool children. The selection was limited to children who had no dental caries, visible plaque, and systemic disease. American Academy of Pediatric Dentistry’s Caries Risk Assessment Tool (CAT) and Clinpro Cario L-Pop (CCLP; 3M ESPE, Germany) were used to categorize children into caries risk groups. Children were classified as being in high-, medium-, or low-risk groups by two methods. Determining risk factors according to CAT were examined. The compliance among the two methods was evaluated. Sixty-eight children were reevaluated intraorally after 3 years. The compliance between CAT and CCLP was sought using Kappa test. Statistical evaluation revealed moderate compliance among methods at baseline. The three most determinant factors in the high-risk group according to CAT were no topical fluoride exposure, no use of dental services, and active caries in mother. CAT is found statistically more successful than CCLP in predicting caries risk 3 years later. Caries risk assessment is effective to predict future caries and can be helpful for the clinician to choose the right tailor-made caries prevention plans.
Effectiveness of a brief motivational intervention to increase the breastfeeding duration in the first six months postpartum: Randomized controlled trial.

AIMS To evaluate the effectiveness of a brief motivational intervention to increase the breastfeeding duration in the first 6 months postpartum in mothers who began breastfeeding in the first hour after birth and to explore the role of general and breastfeeding self-efficacy in this relationship. DESIGN A multi-centre randomized controlled clinical trial. METHODS Data were collected from February 2018 - March 2019. Women were randomly assigned to an intervention group that received a brief motivational intervention (N = 44) or a control group that was offered standard education on breastfeeding (N = 44). Survival analysis techniques were carried out with a follow-up of 6 months. In addition, the roles of breastfeeding and general self-efficacy in the association between BMI and breastfeeding duration were explored through mediation/moderation analysis. RESULTS Among 88 randomized patients (mean age, 32.82 years), 81 (92.04%) completed the trial. The survival analyses of exclusive breastfeeding and breastfeeding (exclusive and non-exclusive) showed reductions in the risk of abandonment in the intervention group of 63% (aHR: 0.37 [0.22 - 0.60] p < .001) and 61% (aHR: 0.39 [0.20 - 0.78] p = .008), respectively. We found that self-efficacy acted as a moderator of the effect of brief motivational intervention on breastfeeding self-efficacy. A discrete indirect effect of brief motivational intervention through increased breastfeeding self-efficacy was found on breastfeeding duration, with an index of moderated mediation of 0.08 (95% CI: 0.02 - 0.19). Greater positive variations in the breastfeeding self-efficacy scores during follow-up predicted exclusive and non-exclusive breastfeeding duration. CONCLUSION A brief motivational intervention conducted in the immediate postpartum period increases breastfeeding and exclusive breastfeeding duration in the first 6 months. Although breastfeeding self-efficacy seems to moderate the effect of brief motivational intervention on the increase in breastfeeding duration, other factors may influence its effectiveness. Further studies could focus on exploring how brief motivational intervention works and whether it also works for pregnant mothers who have not yet decided whether to breastfeed. IMPACT Brief motivational intervention could be introduced as part of routine care of women who begin breastfeeding to improve the low rates of exclusive breastfeeding at six months postpartum.
Acute Effects of Resistance Exercise and the Use of GH or IGF-1 Hormones on Oxidative Stress and Antioxidant Markers in Bodybuilders

The aim of this study was to examine the influence of peptide hormone use on oxidative stress and antioxidant responses to a single session of resistance exercise in male bodybuilders. Forty-five male bodybuilders were divided into three groups: bodybuilders using growth hormone for at least 1 year (i.e., 3 to 4 times in the year) (GH-user, n = 15), bodybuilders using insulin-like growth factor-1 for at least 1 year (i.e., 3 to 4 times in the year) (IGF-1-user, n = 15), and peptide hormone-free bodybuilders (Non-user, n = 15). The heavy resistance exercise protocol consisted of five sets with 80% of one repetition maximum for six exercises. Blood samples were obtained pre and post heavy resistance exercise (HRE) in order to evaluate changes in oxidative stress (8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO)) and antioxidant biomarkers (glutathione peroxidase (GPx), catalase (CAT) and glutamate (GLU)) level. All the experimental groups showed increases in MDA (p = 0.038), NO (p = 0.028), GPx (p = 0.012), and GLU (p = 0.003) concentrations after resistance exercise. For 8-OHdG, the Non-user and IGF-1-user groups indicated increases at post-exercise (p = 0.001), without any significant changes in the GH-user group (p = 0.87). In addition, the changes in serum GPx and GLU levels were greater for the GH-user group than the Non-user and IGF-1-user groups (p = 0.001). In conclusion, HRE induced significant increases in 8-OHdG (except to GH-user group), MDA, NO, GPx, and GLU levels with greater changes in GPx and GLU for the GH-user group.
Endothelial Bmp2 knockout exacerbates hemochromatosis in Hfe knockout mice but not Bmp6 knockout mice

Bone morphogenetic proteins BMP2 and BMP6 play key roles in systemic iron homeostasis by regulating production of the iron hormone hepcidin. HFE also regulates hepcidin through a mechanism that intersects with the BMP-SMAD1/5/8 pathway. However, the relative roles of BMP2 compared with BMP6 and whether HFE regulates hepcidin through a BMP2-dependent mechanism remain uncertain. We therefore examined the iron phenotype of mice deficient for both Bmp2 and Bmp6 or both Bmp2 and Hfe compared with single knockout (KO) mice and littermate controls. Eight-week-old double endothelial Bmp6/Bmp2 KO mice exhibited a similar degree of hepcidin deficiency, serum iron overload, and tissue iron overload compared with single KO mice. Notably, dietary iron loading still induced liver SMAD5 phosphorylation and hepcidin in double Bmp6/endothelial Bmp2 KO mice, although no other BMP ligand mRNAs were increased in the livers of double KO mice, and only Bmp6 and Bmp2 mRNA were induced by dietary iron loading in wildtype mice. In contrast, double Hfe/endothelial Bmp2 KO mice exhibited reduced hepcidin and increased extrahepatic iron loading compared to single Hfe or endothelial Bmp2 KO mice. Liver phosphorylated SMAD5 and the SMAD1/5/8 target Id1 mRNA were also reduced in double Hfe/endothelial Bmp2 KO compared with single endothelial Bmp2 KO females. Finally, hepcidin and Id1 mRNA induction by homodimeric BMP2, homodimeric BMP6, and heterodimeric BMP2/6 were blunted in Hfe KO primary hepatocytes. Conclusion— These data suggest that BMP2 and BMP6 work collaboratively to regulate hepcidin expression, that BMP2- and BMP6-independent SMAD1/5/8 signaling contributes a non-redundant role to hepcidin regulation by iron, and that HFE regulates hepcidin at least in part through a BMP2-independent, but SMAD1/5/8-dependent, mechanism. exogenously added homodimeric BMP2 and BMP6 ligand can stimulate hepcidin expression both in vitro and in vivo Future studies will be to understand why endogenous BMP6 and BMP2 fail to appreciably compensate for the loss of the other in endothelial Bmp2 and Bmp6 KO mice respectively, and whether endogenous heterodimeric BMP2/6 ligand has an important function role in hepcidin regulation and systemic iron homeostasis. In summary, our results demonstrate that BMP2 and BMP6 predominantly work together to regulate hepcidin expression and that HFE regulates hepcidin at least in part through a BMP2-independent, but SMAD-dependent, pathway. Our results also support the existence of additional BMP ligand(s) that play a role in iron-mediated hepcidin regulation to maintain systemic iron homeostasis. These findings provide new insights into how iron regulates hepcidin production and how this process is impaired in hereditary hemochromatosis.
In Vivo AAV-CRISPR/Cas9–Mediated Gene Editing Ameliorates Atherosclerosis in Familial Hypercholesterolemia

Supplemental Digital Content is available in the text. Background: Mutations in low-density lipoprotein (LDL) receptor (LDLR) are one of the main causes of familial hypercholesterolemia, which induces atherosclerosis and has a high lifetime risk of cardiovascular disease. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is an effective tool for gene editing to correct gene mutations and thus to ameliorate disease. Methods: The goal of this work was to determine whether in vivo somatic cell gene editing through the CRISPR/Cas9 system delivered by adeno-associated virus (AAV) could treat familial hypercholesterolemia caused by the Ldlr mutant in a mouse model. We generated a nonsense point mutation mouse line, LdlrE208X, based on a relevant familial hypercholesterolemia–related gene mutation. The AAV-CRISPR/Cas9 was designed to correct the point mutation in the Ldlr gene in hepatocytes and was delivered subcutaneously into LdlrE208X mice. Results: We found that homogeneous LdlrE208X mice (n=6) exhibited severe atherosclerotic phenotypes after a high-fat diet regimen and that the Ldlr mutation was corrected in a subset of hepatocytes after AAV-CRISPR/Cas9 treatment, with LDLR protein expression partially restored (n=6). Compared with the control groups (n=6 each group), the AAV-CRISPR/Cas9 with targeted single guide RNA group (n=6) had significant reductions in total cholesterol, total triglycerides, and LDL cholesterol in the serum, whereas the aorta had smaller atherosclerotic plaques and a lower degree of macrophage infiltration. Conclusions: Our work shows that in vivo AAV-CRISPR/Cas9–mediated Ldlr gene correction can partially rescue LDLR expression and effectively ameliorate atherosclerosis phenotypes in Ldlr mutants, providing a potential therapeutic approach for the treatment of patients with familial hypercholesterolemia.