Miracle-dz/UniFAR-FTUs huggingface dataset:
Incidence of inflammatory bowel disease and phenotype at diagnosis in 2011: results of Epi-IBD 2011 study in the Vigo area.

OBJECTIVE To validate the incidence of inflammatory bowel disease (IBD) reported in Vigo in 2010 within the Epi-IBD study, which was the highest incidence reported so far in Spain. METHODS Epidemiological, prospective, population-based inception cohort study. All incident cases of IBD from 1st January to 31st December 2011 and living in the Vigo area at diagnosis were included. RESULTS 100 patients were diagnosed (62% men, median age 43.27 years): 49 ulcerative colitis (UC), 34 Crohn's disease (CD) and 17 IBD unclassified (IBDU). The incidence (per 100,000 inhabitants/year) was 17.56 (CD: 5.97; UC: 8.60; IBDU: 2.98), similar to that reported in 2010. The incidence in non-pediatric population was 19.66 (CD: 6.89, UC: 9.52; IBDU: 3.04). CD and UC phenotype was similar in 2010 and 2011. CONCLUSION This study supports the increase of incidence of EII in the Vigo area reported in 2010.
Chemically Stabilized DNA Barcodes for DNA‐Encoded Chemistry

Abstract DNA‐encoded compound libraries are a widely used small molecule screening technology. One important aim in library design is the coverage of chemical space through structurally diverse molecules. Yet, the chemical reactivity of native DNA barcodes limits the toolbox of reactions for library design. Substituting the chemically vulnerable purines by 7‐deazaadenine, which exhibits tautomerization stability similar to natural adenine with respect to the formation of stable Watson–Crick pairs, yielded ligation‐competent, amplifiable, and readable DNA barcodes for encoded chemistry with enhanced stability against protic acid‐ and metal ion‐promoted depurination. The barcode stability allowed for straightforward translation of 16 exemplary reactions that included isocyanide multicomponent reactions, acid‐promoted Pictet–Spengler and Biginelli reactions, and metal‐promoted pyrazole syntheses on controlled pore glass‐coupled barcodes for diverse DEL design. The Boc protective group of reaction products offered a convenient handle for encoded compound purification.
Climate and land-use changes interact to drive long-term reorganization of riverine fish communities globally

Significance Understanding the mechanisms by which biological communities are reorganized by environmental change is a key question facing ecologists. Using a global database of fish abundance time series spanning recent decades, together with community-level indices describing species temperature and flow affinities, we show that two important aspects of climate change (water temperature and streamflow alteration) are interacting with land-use modification to drive increases in the dominance of species that prefer warm- and slow-water habitats. Although these community reorganizations show substantial geographical variation, they can be explained by a combination of degree of environmental changes and initial community composition. These findings offer insights to improve ecological forecasting in the future to better inform and prioritize conservation actions in freshwater ecosystems. As climate change unfolds, changes in population dynamics and species distribution ranges are expected to fundamentally reshuffle communities worldwide. Yet, a comprehensive understanding of the mechanisms and extent of community reorganization remains elusive. This is particularly true in riverine systems, which are simultaneously exposed to changing temperature and streamflow, and where land-use change continues to be a major driver of biodiversity loss. Here, we use the most comprehensive compilation of fish abundance time series to date to provide a global synthesis of climate- and LU-induced effects on riverine biota with respect to changes in species thermal and streamflow affinities. We demonstrate that fish communities are increasingly dominated by thermophilic (warm-water) and limnophilic (slow-water) species. Despite being consistent with trends in water temperature and streamflow observed over recent decades, these community changes appear largely decoupled from each other and show wide spatial variation. We further reveal a synergy among climate- and land use-related drivers, such that community thermophilization is heightened in more human-modified systems. Importantly, communities in which species experience thermal and flow regimes that approach or exceed their tolerance thresholds (high community sensitivity), as well as species-poor communities (low community resilience), also display faster rates of compositional change. This research illustrates that quantifying vulnerability of riverine systems to climate change requires a broadening from a narrower thermal focus to more integrative approaches that account for the spatially varying and multifaceted sensitivity of riverine organisms to the interactive effects of water temperature, hydrology, and other anthropogenic changes.
A Nonlethal Murine Flame Burn Model Leads to a Transient Reduction in Host Defenses and Enhanced Susceptibility to Lethal Pseudomonas aeruginosa Infection

Of the 486,000 burn injuries that required medical treatment in the United States in 2016, 40,000 people were hospitalized, with >3,000 fatalities. After burn injury, humans are at increased risk of sepsis and mortality from infections caused by Pseudomonas aeruginosa, an opportunistic pathogen. ABSTRACT Of the 486,000 burn injuries that required medical treatment in the United States in 2016, 40,000 people were hospitalized, with >3,000 fatalities. After burn injury, humans are at increased risk of sepsis and mortality from infections caused by Pseudomonas aeruginosa, an opportunistic pathogen. We hypothesize that systemic events were initiated from the burn that increased the host’s susceptibility to P. aeruginosa. A nonlethal 10% total body surface area (TBSA), full-thickness flame burn was performed in CD-1 mice without and with subsequent P. aeruginosa (strain M2) infection. The 50% lethal dose for subcutaneous infection with P. aeruginosa M2 at the burn site immediately after the burn decreased by 6 log, with mortality occurring between 18 and 26 h, compared with P. aeruginosa-infected mice without burn injury. Bacteria in distal organs were detected by 18 h, concurrent with the onset of clinical symptoms. Serum proinflammatory cytokines (interleukin-6 [IL-6], IL-1β, gamma interferon, and tumor necrosis factor alpha) and the anti-inflammatory cytokine IL-10 were first detected at 12 h postburn with infection and continued to increase until death. Directly after burn alone, serum levels of HMGB1, a danger-associated molecular pattern and TLR4 agonist, transiently increased to 50 ng/ml before returning to 20 ng/ml. Burn with P. aeruginosa infection increased serum HMGB1 concentrations >10-fold (250 ng/ml) at the time of death. This HMGB1-rich serum stimulated TLR4-mediated NF-κB activation in a TLR4 reporter cell line. Treatment of infected burned mice with P5779, a peptide inhibitor of HMGB1, increased the mean survival from 23 to 42 h (P < 0.0001). We conclude that the high level of serum HMGB1, which preceded the increase in proinflammatory cytokines, is associated with postburn mortality.
The promising roles of medicinal plants and bioactive compounds on hepatic lipid metabolism in the treatment of non-alcoholic fatty liver disease in animal models: molecular targets

Abstract Imbalance in hepatic lipid metabolism can lead to an abnormal triglycerides deposition in the hepatocytes which can cause non-alcoholic fatty liver disease (NAFLD). Four main mechanisms responsible for regulating hepatic lipid metabolism are fatty acid uptake, de novo lipogenesis, lipolysis and fatty acid oxidation. Controlling the expression of transcription factors at molecular level plays a crucial role in NAFLD management. This paper reviews various medicinal plants and their bioactive compounds emphasising mechanisms involved in hepatic lipid metabolism, other important NAFLD pathological features, and their promising roles in managing NAFLD through regulating key transcription factors. Although there are many medicinal plants popularly investigated for NAFLD treatment, there is still little information and scientific evidence available and there has been no research on clinical trials scrutinised on this matter. This review also aims to provide molecular information of medicinal plants in NALFD treatment that might have potentials for future scientifically controlled studies.
Differences between Young Children’s Actual, Self-perceived and Parent-perceived Aquatic Skills

As drowning is a leading cause of unintentional injury/death in children worldwide, perceptions of their actual aquatic skills are of critical importance. Children’s self-perceptions may influence the risks they take, and parental perceptions may influence the degree of supervision deemed to be necessary for children in and around water. Accordingly, we examined the differences between young children’s actual, self-perceived and parent-perceived aquatic skills. Using a three-way repeated measures ANCOVA, we analyzed data from 134 child-parent dyads (56.0% boys; M age = 7.1, SD = 1.1 years; and 71.6% mothers). We measured self and parental perceptions of the child’s aquatic skills with the ‘Pictorial Scale of Perceived Water Competence’ (PSPWC), and we applied the exact same 17 test items of the PSPWC to assess the child’s actual aquatic skill level in the water. Controlling for years of swimming school experience, within-subject differences between the total scores on the ‘Actual Aquatic Skills Test’ (AAST) and both the child- and parent-completed PSPWC indicated lower than actual estimates of the children’s aquatic skill level. The degree of disagreement against the AAST was more pronounced in parents than in 6-7 year-old children but was similar between parents and 8-9 year-old children, with these patterns being evident regardless of the children’s sex. Our study contributes to an ongoing validation of the PSPWC and represents a key advance in assessing and comparing children’s actual and perceived aquatic skill competence, using perfectly aligned instruments. Future research and practice might explore children’s actual aquatic skills in different contexts (e.g., open water), include perspectives of non-parent caregivers and assess perceived and actual water competence across development.
Effects of Coronavirus Disease Pandemic on Tuberculosis Notifications, Malawi

The coronavirus disease (COVID-19) pandemic might affect tuberculosis (TB) diagnosis and patient care. We analyzed a citywide electronic TB register in Blantyre, Malawi and interviewed TB officers. Malawi did not have an official COVID-19 lockdown but closed schools and borders on March 23, 2020. In an interrupted time series analysis, we noted an immediate 35.9% reduction in TB notifications in April 2020; notifications recovered to near prepandemic numbers by December 2020. However, 333 fewer cumulative TB notifications were received than anticipated. Women and girls were affected more (30.7% fewer cases) than men and boys (20.9% fewer cases). Fear of COVID-19 infection, temporary facility closures, inadequate personal protective equipment, and COVID-19 stigma because of similar symptoms to TB were mentioned as reasons for fewer people being diagnosed with TB. Public health measures could benefit control of both TB and COVID-19, but only if TB diagnostic services remain accessible and are considered safe to attend.
Immune-related adverse events as clinical biomarkers in metastatic renal cell carcinoma patients treated with immune checkpoint inhibitors.

BACKGROUND Immune checkpoint inhibitors (ICI) are an important treatment for metastatic renal cell carcinoma (mRCC). These agents may cause immune-related adverse events (irAEs) and the relationship between irAEs and outcomes is poorly understood. We investigated the association between irAEs and clinical outcomes in ICI-treated mRCC patients. METHODS We performed a retrospective study of 200 ICI-treated mRCC patients at Winship Cancer Institute from 2015-2020. Data on irAEs were collected from clinic notes and laboratory values and grades were determined using common terminology criteria in adverse events (CTCAE) version 5.0. The association with overall survival (OS) and progression-free survival (PFS) was modeled by Cox proportional hazards model. Logistic regression models were used to define odds ratios for clinical benefit (CB). Landmark analysis and extended Cox models were used to mitigate lead-time bias by treating irAEs as a time-varying covariate. RESULTS Most patients (71.0%) were males and one-third of patients (33.0%) experienced ≥1 irAE, most commonly involving the endocrine glands (13.0%), gastrointestinal tract (10.5%), or skin (10.0%). Patients who experienced irAEs had significantly longer OS (HR=0.52, p=0.013), higher chance of CB (OR=2.10, p=0.023) and showed a trend towards longer PFS (HR=0.71, p=0.065) in multivariate analysis. Patients who had endocrine irAEs, particularly thyroid irAEs, had significantly longer OS, PFS, and higher chance of CB. In 14-week landmark analysis, irAEs were significantly associated with prolonged OS (p=0.045). Patients who experienced irAEs had significantly longer median OS (44.5 versus 18.2 months, p=0.005) and PFS (7.5 versus 3.6 months, p=0.003) without landmark compared to patients who did not. CONCLUSIONS We found that ICI-treated mRCC patients who experienced irAEs, particularly thyroid irAEs, had significantly improved clinical outcomes compared to patients who did not have irAEs. This suggests that irAEs may be effective clinical biomarkers in ICI-treated mRCC patients. Future, prospective studies are warranted to validate these findings. IMPLICATIONS FOR PRACTICE We found that early onset immune-related adverse events (irAEs) are associated with significantly improved clinical outcomes in metastatic renal cell carcinoma (mRCC) patients treated with immune checkpoint inhibitors (ICI). In our site-specific irAE analysis, endocrine irAEs, particularly thyroid irAEs, were significantly associated with improved clinical outcomes. These results have implications for practicing medical oncologists given the increasing utilization of ICIs for the treatment of mRCC. Importantly, these results suggest that early irAEs and thyroid irAEs at any time on treatment with ICIs may be clinical biomarkers of clinical outcomes in mRCC patients treated with ICIs.
Quality of life among relatives of patients with amyotrophic lateral sclerosis: A prospective and longitudinal study

Abstract Objective Relatives are often central in caring for patients with amyotrophic lateral sclerosis (ALS), involving considerable physical, emotional, and social challenges. The aim of this study was to describe individual quality of life (iQoL) among relatives of patients with ALS, from diagnosis through disease progression. Method A total of 31 relatives were included. Data collection was performed at five time points: 1–3 months after their relatives had been diagnosed with ALS and every 6 months for 2 years. Quality of life was determined using the Schedule of Evaluation of Individual Quality of Life — Direct Weighting (SEIQoL-DW), emotional distress with the Hospital Anxiety and Depression Scale (HADS), and the illness severity of the patients was determined with the Amyotrophic Lateral Sclerosis Functional Rating Scale (ALS FRS-R). Results The SEIQoL-DW involves participants nominating the important life areas. The most nominated areas were family, friends, health, and leisure. Although most relatives had overall good and stable iQoL, several had scores indicating poor iQoL on some occasions during the disease trajectory. The relatives’ iQoL correlated with emotional well-being and the patient's physical function at different time points. Significant of result Social relations, emotional well-being, and rapid decline in the patient's physical function influence the relatives’ iQoL. Measuring both emotional well-being and iQoL, with a focus on the relatives’ own descriptions of perceived iQoL and those factors contributing to their iQoL during the disease trajectory may improve the possibility of identifying and supporting those relatives with poor iQoL.
Remission of chronic spontaneous urticaria following omalizumab with gradually extended dosing intervals: Real‐life data

Omalizumab is a well‐established treatment option in chronic spontaneous urticaria unresponsive to antihistamines at standard or higher doses. However, characteristics of the remission and relapse following the withdrawal of omalizumab remain largely unknown. We aimed to define the characteristics of remission in CSU following omalizumab with gradually lengthened dosing intervals in this retrospective study of 102 patients who were treated with at least 3 doses of omalizumab between 2013 and 2020. Of 102 patients, 70 (68.6%) showed a CR to omalizumab at standard doses. Omalizumab could be discontinued in 47 of 70 patients using gradually lengthened dosing intervals. Following a mean follow‐up duration of 12.2 months, 25 (58.1%) patients were still in remission while 18 (41.9%) had relapse (Follow‐up data were not available in 4 patients). The relapses were unresponsive to antihistamines in 14 patients (77.7%), however, re‐treatment with omalizumab led to complete control of symptoms. The patients younger than 40 were more likely to relapse. Despite the need for comparison with fixed‐dosing intervals in larger, prospective studies, the results of this study imply that omalizumab with gradually extended dosing intervals might provide a long duration of remission in CSU.
Solubility Measurement of Natural Gas in Reservoir Formation Water under (333.2 to 393.2) K and (15.0 to 43.6) MPa

The solubility of five groups of natural gas in reservoir formation water was measured under (333.2 to 393.2) K and (15.0 to 43.6) MPa. The formation water and corresponding natural gas were sampled in situ from a China oilfield. The range of total dissolved solids (TDS) of the reservoir formation water samples is from (8744 to 80634) mg·kg−1. The mole fraction of methane in natural gas ranges from 0.7575 to 0.9449. The experimental results show that, with the increase of temperature and pressure, the solubility of natural gas in formation water goes through a minimum value under the experimental conditions. The solubility of natural gas in formation water was also influenced by a composition of gas phase and TDS of liquid phase. The salt effect becomes more significant in the high-pressure region.
Individual differences in ethics positions: The EPQ-5

We revised the Ethics Position Questionnaire (EPQ), which measures variations in sensitivity to harm (idealism) and to moral standards (relativism). Study 1 identified the core components of the measured constructs theoretically and verified those features through confirmatory factor analysis (n = 2,778). Study 2 replicated these findings (n = 10,707), contrasted the theoretically defined two-factor model to alternative models, and tested for invariance of factor covariances and mean structures for men and women. Study 3 examined the relationship between the EPQ and related indicators of ethical thought (values and moral foundations) and the theory’s four-fold classification typology of exceptionists, subjectivists, absolutists, and situationists. The three studies substantially reduced the original EPQ’s length, clarified the conceptual interpretation of the idealism and relativism scales, affirmed the EPQ’s predictive and convergent validity, and supported the four-fold classification of individuals into ethics positions. Implications for previous findings and suggestions for future research are discussed.
Confirmed Cases of Ophidiomycosis in Museum Specimens from as Early as 1945, United States

Ophidiomycosis represents a conservation threat to wild snake populations. The disease was reported in North America early in the 21st century, but the history of ophidiomycosis has not been investigated. We examined museum specimens and confirmed cases of ophidiomycosis >50 years before the disease’s reported emergence.
The ratio of neutrophil to lymphocyte predicts interstitial lung disease and its prognosis in patients with primary Sjögren's syndrome: a retrospective analysis.

BACKGROUND During the course of disease, some patients with primary Sjögren's syndrome (pSS) develop interstitial lung disease (ILD), which leads to a poor prognosis. There is currently a lack of methods to identify high-risk patients with ILD and predict the prognosis. The purpose of this study was to analyze the predictive value of the neutrophil to lymphocyte ratio (NLR) for the occurrence of ILD and its prognosis in patients with pSS. METHODS According to the inclusion and exclusion criteria, patients with pSS admitted to our hospital from May 2009 to November 2020 were included. The patients were divided into either an ILD group or a non-interstitial pneumonia [non-ILD (NILD)] group. We compared the baseline data of the two groups of participants, and the participants were followed up (≥1 year) at clinic visits. Logistic multivariate regression analysis was used to analyze the risk factors related to ILD and prognosis in patients with pSS. RESULTS A total of 217 patients with pSS were included, of which 71 (32.7%) participants were diagnosed with ILD (ILD group) at the time of pSS diagnosis, and 146 (67.3%) participants had no obvious ILD (NILD group). Based on follow-up results, logistic multivariate analysis revealed that NLR [relative risk (RR) =1.81, 95% confidence interval (CI): 1.15 to 4.73], age (RR =1.43, 95% CI: 1.06 to 3.66), non-regular treatment (RR =1.39, 95% CI: 1.03 to 3.38), ALB <35 g/L (RR =1.32, 95% CI: 1.05 to 3.17), and elevated CRP (RR =1.44, 95% CI: 1.10 to 4.53) were associated with the occurrence of ILD in participants with pSS during followup. Age (RR =1.28, 95% CI: 1.06 to 2.25), NLR (RR =1.43, 95% CI: 1.12 to 2.57), non-regular treatment (RR =1.51, 95% CI: 1.18 to 3.01), and ILD (RR =2.05, 95% CI: 1.36 to 4.72) were related to all-cause death during follow-up. CONCLUSIONS The NLR is a risk factor for ILD in patients with pSS. The higher the NLR, the worse the prognosis; ILD significantly increases the risk of death in patients with pSS.