Miracle-dz/UniFAR-FTUs huggingface dataset:
Consumption Context Effects on Fine Wine Consumer Segments’ Liking and Emotions

Wine consumer lifestyle segmentation has been widely studied; however, most studies have solely utilised online surveys. This work investigated the impact of context on wine consumer segments’ liking and emotions while consuming wines in different environments. Two studies were conducted with regular wine consumers segmented based on their fine wine behaviour using the Fine Wine Instrument. Study 1 (n = 122) investigated the effects of wine variety and product information, and Study 2 (n = 346) the effects of wine quality and consumption context, on hedonic and emotional responses of the segments. Within both studies, three segments were identified and named: Wine Enthusiasts, Aspirants and No Frills. The Wine Enthusiast segment generally liked the wines more and perceived more intense positive emotions when consuming wine compared to the No Frills segment, with the Aspirant’s likes and emotion intensities ranging in between. Wine Enthusiasts were more discriminative of their preferred wines and reported stronger positive emotions when tasting higher quality (Study 1) and more complex (Study 2) wines. The consistent results across the two studies showed for the first time that consumer segments, based on lifestyle segmentation, differ in their hedonic and emotional responses towards wine when actually tasting wines, demonstrating that the Fine Wine Instrument has practical implications and can identify wine consumers displaying different wine consumption behaviours.
Overview of the Potential Impacts of Climate Change on the Microbial Safety of the Dairy Industry

Climate change is expected to affect many different sectors across the food supply chain. The current review paper presents an overview of the effects of climate change on the microbial safety of the dairy supply chain and suggest potential mitigation strategies to limit the impact. Raw milk, the common raw material of dairy products, is vulnerable to climate change, influenced by changes in average temperature and amount of precipitation. This would induce changes in the microbial profile and heat stress in lactating cows, increasing susceptibility to microbial infection and higher levels of microbial contamination. Moreover, climate change affects the entire dairy supply chain and necessitates adaptation of all the current food safety management programs. In particular, the review of current prerequisite programs might be needed as well as revisiting the current microbial specifications of the receiving dairy products and the introduction of new pretreatments with stringent processing regimes. The effects on microbial changes during distribution and consumer handling also would need to be quantified through the use of predictive models. The development of Quantitative Microbial Risk Assessment (QMRA) models, considering the whole farm-to-fork chain to evaluate risk mitigation strategies, will be a key step to prioritize actions towards a climate change-resilient dairy industry.
Intra-renal pressure and temperature during ureteroscopy: Does it matter?

Kidney stone prevalence has raised up in past few years (1-3). Several factors contributed to this growth rate including professional journey, dietetics habits and metabolic syndrome including obesity and Diabetes Mellitus (4, 5). Moreover, urolithiasis is a chronic disease with a recurrence rate close to 50% in 5 years (6, 7). Frequently, urolithiasis treatment requires surgical intervention. Endoscopic approach is the gold standard and retrograde intra-renal surgery (RIRS) and semi-rigid ureteroscopy are the most common procedures performed for kidney and ureteral stones removal (8, 9). New technology including digital and disposable flexible ureteroscopes, well designed guidewires, baskets, access sheaths and high-power laser systems with small fibers are particularly interesting devices to guarantee safety and attractivity for retrograde approach. Ureteroscopy’s complications are rare (10), but urothelial lesions, peri-renal collections, infection with or without sepsis can be related to surgical technique. Ureteroscopy is normally performed with fluid irrigation and intra-renal temperature and pressure must be taken in consideration due the fact it may correlate to complications, especially sepsis risk. Our paper aims to review ureteroscopy surgical aspects regarding intra-renal pressure/temperature and the relationship between this parameters and potential complication involved. Intra-renal pressure Physiological intra-renal pressure ranges from 5 to 10cm H2O and it is increased during endoscopic surgery. Some studies reported 40cm H2O as the safe upper cut off for intra-operative pressure (11). Complication rate increases in higher intra-renal pressure scenarios. Saline irrigation influx control and ureteral access sheath use are the main determinants to avoid the increasing in intra-renal pressure during ureteroscopy. Saline irrigation plays a major role during ureteroscopy, leading to ureteral lumen expansion and permitting renal pelvis and calices visualization and ureteroscope free navigation through the entire collecting system. Historically, ureteroscopes first generations were not equipped with a robust working channel and no irrigation was possible. Intra-operative diuretics were commonly used to minimize the effects of irrigation deficit (12). Upgrade in ureteroscopes technology added irrigation devices for working channels and improved surgeon visualization. However, saline flow can potentially increase intra-renal pressure (11, 13). As previously mentioned, pressure of 40cm of H2O or higher can increase post-operative complication risks, including bleeding, perirenal collection, and sepsis (14). Sepsis is probably explained by pyelo-venous reflux mechanism, translocating bacteria from urinary colleting system into the blood stream. There are several ways to use saline to irrigate during ureteroscopy and gravity irrigation is Intra-renal pressure and temperature during ureteroscopy: Does it matter? _______________________________________________
COVID‐19 associated Kikuchi‐Fujimoto disease

We discuss the case of a 17-year-old male who presented with painful lymphadenopathy, fevers, weight loss, anorexia and a history of preceding anosmia. Histological features on lymph node biopsy were in-keeping with Kikuchi-Fujimoto disease (KFD). We later confirmed SARS-CoV-2 (COVID-19) infection on antibody testing. This case describes an atypical presentation of COVID-19 in a young patient and highlights an association between the virus and this rare disease.
Lignans and Gut Microbiota: An Interplay Revealing Potential Health Implications

Plant polyphenols are a broad group of bioactive compounds characterized by different chemical and structural properties, low bioavailability, and several in vitro biological activities. Among these compounds, lignans (a non-flavonoid polyphenolic class found in plant foods for human nutrition) have been recently studied as potential modulators of the gut–brain axis. In particular, gut bacterial metabolism is able to convert dietary lignans into therapeutically relevant polyphenols (i.e., enterolignans), such as enterolactone and enterodiol. Enterolignans are characterized by various biologic activities, including tissue-specific estrogen receptor activation, together with anti-inflammatory and apoptotic effects. However, variation in enterolignans production by the gut microbiota is strictly related to both bioaccessibility and bioavailability of lignans through the entire gastrointestinal tract. Therefore, in this review, we summarized the most important dietary source of lignans, exploring the interesting interplay between gut metabolites, gut microbiota, and the so-called gut–brain axis.
Development and validation of a nomogram using on admission routine laboratory parameters to predict in‐hospital survival of patients with COVID‐19

To develop and validate a nomogram using on admission data to predict in‐hospital survival probabilities of coronavirus disease 2019 (COVID‐19) patients. We analyzed 855 COVID‐19 patients with 52 variables. The least absolute shrinkage and selection operator regression and multivariate Cox analyses were used to screen significant factors associated with in‐hospital mortality. A nomogram was established based on the variables identified by Cox regression. The performance of the model was evaluated by C‐index and calibration plots. Decision curve analysis was conducted to determine the clinical utility of the nomogram. Six variables, including neutrophil (hazard ratio [HR], 1.088; 95% confidence interval [CI], [1.0004–1.147]; p < .001), C‐reactive protein (HR, 1.007; 95% CI, [1.0026–1.011]; p = .002), IL‐6 (HR, 1.001; 95% CI, [1.0003–1.002]; p = .005), d‐dimer (HR, 1.034; 95% CI, [1.0111–1.057]; p = .003), prothrombin time (HR 1.086, 95% CI [1.0369–1.139], p < .001), and myoglobin (HR, 1.001; 95% CI, [1.0007–1.002]; p < .001), were identified and applied to develop a nomogram. The nomogram predicted 14‐day and 28‐day survival probabilities with reasonable accuracy, as assessed by the C‐index (0.912) and calibration plots. Decision curve analysis showed relatively wide ranges of threshold probability, suggesting a high clinical value of the nomogram. Neutrophil, C‐reactive protein, IL‐6, d‐dimer, prothrombin time, and myoglobin levels were significantly correlated with in‐hospital mortality of COVID‐19 patients. Demonstrating satisfactory discrimination and calibration, this model could predict patient outcomes as early as on admission and might serve as a useful triage tool for clinical decision making.
Activation of silent information regulator 1 exerts a neuroprotective effect after intracerebral hemorrhage by deacetylating NF‐κB/p65

Nuclear factor (NF)‐κB‐mediated neuroinflammation is an important mechanism of intracerebral hemorrhage (ICH)‐induced neurotoxicity. Silent information regulator 1 (SIRT1) plays a multi‐protective effect in a variety of diseases by deacetylating and inhibiting NF‐κB/p65. However, the role of SIRT1 in brain damage following ICH remains unclear. We hypothesized that SIRT1 can protect against ICH‐induced brain damage by inhibiting neuroinflammation through deacetylating NF‐κB/p65. The ICH model was induced in vivo (with collagenase) and in vitro (with hemoglobin). Resveratrol and Ex527 were administered to activate or inhibit SIRT1, respectively. Western blot, immunohistochemistry, and immunofluorescence assays were performed to detect the expression of SIRT1 and p65. Enzyme‐linked immunosorbent assays (ELISAs) were used to explore tumor necrosis factor (TNF)‐α and interleukin (IL)‐1β release. The neurological score, brain water content, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and brain hemoglobin content were determined to evaluate the neuroprotective effect of SIRT1. SIRT1 expression was decreased, whereas the level of acetylated p65 (Ac‐p65) was elevated after ICH in vivo. Moreover, hemoglobin treatment decreased the expression of SIRT1 in vitro. Activation of SIRT1 by resveratrol had a neuroprotective effect, along with decreased levels of Ac‐p65, IL‐1β, TNF‐α, and apoptosis after ICH. The effect of resveratrol was abolished by the SIRT1 inhibitor Ex527. Our results are consistent with the hypothesis that SIRT1 exerts a neuroprotective effect after ICH by deacetylating p65 to inhibit the NF‐κB‐dependent inflammatory response.
The frequency of Pseudomonas aeruginosa bacteria with some pathogenic bacteria in burns injuries and study their resistance to antibiotics

90 swabs taken from burn wounds of patients admitted to and attending burn unit in Azadi teaching hospital in Kirkuk from the period (14/12/2014 to 19/3/2015). The age of patients ranged from 11 months to 77 years the burn wounds infected in both sexes of patients, but females more than male (40 females, 27 male). The variable degrees of burn was from first to third degree was observed in the 90 patients. Some of the samples (26.86%) from revealed a mixed bacterial infection and 73.13% were single bacterial infection. Different gram positive and gram negative bacterial were isolated in different percentages as staph. aureus was isolated from 12.79% of cultures, staph. epidermidis isolated in 9.30%. While gram negative bacteria was isolated as follows (pseudomonas aeruginosa in 32.55%, klebsiella pneumoniae and Enterobacter cloacae in 17.44% for each, E.coli in 8.13% and proteus mirabilis in 2.32%). P. aeruginosa was the highest percentage of the all gram positive and gram negative bacteria isolates has been obtained 28 isolation percentage 32.5% from all 86 isolates. P. aeruginosa was isolated from single and mixed isolation (gram negative and gram positive) bacteria (E. cloacae, E.coli, K. Pneumoniae, P. mirabilis, S. aureus, S.spidermidis). Regarding the sensitivity of the P. aeruginosa from the cultures to different antibiotics , Twelve antibacterial agents were used including (Cefotaxime , Imipenem , Gentamicin , Amoxicillin , Auqmentin , Ampicillin , Ceftriaxone , Ceftazidime , Carbencillin , Amikacin , Ciprofloxacin , Tetracyclin ). Out of these antibiotics Pseudomonas aeruginosa were more resistant about 100% to (Augmentin , Amoxicillin , Ampicillin( , 93% to the (Ceftriaxone , Cefotaxime , Carbenicillin , Imipenem Kirkuk University Journal /Scientific Studies (KUJSS) Volume 12, Issue 1, January 2017 ISSN 1992 – 0849 Email: kirkukjoursci@yahoo.com, www.kujss. com Web Site: kirkukjoursci@gmail.com 124 Gentamicin, Tetracyclin ) , 89% Ciprofloxacin, while it found less than (64-57)% Amikacin and Ceftazidime respectively. When comparing multiple antibiotic resistance among bacteria isolated from the mixed and individual infections there was a difference in the results, where the proportion of resistant mixed infections for (12) antibiotic was rates of 27.7% while the resistance of individual infection ratio Pseudomonas aeruginosa (12) antibiotic at 23-52 %.
High-Dose Vitamin D Supplementation in Pregnancy and Neurodevelopment in Childhood

Key Points Question Does maternal high-dose vitamin D supplementation in the third trimester of pregnancy improve offspring neurodevelopment in the first 6 years of life? Findings This prespecified secondary analysis of a randomized clinical trial of vitamin D3 supplementation during pregnancy and offspring neurodevelopment among 551 children showed no effect on neurodevelopment in the first 6 years of life, except for an isolated negative effect on language development at age 2 years in the high-dose compared with standard dose group. Meaning This secondary analysis of a randomized clinical trial found that maternal high-dose vitamin D3 supplementation during pregnancy did not improve neurodevelopmental outcomes in the offspring during the first 6 years of life compared with the standard recommended dose of vitamin D.
The interactions between mTOR and NF‐κB: A novel mechanism mediating mechanical stretch‐stimulated osteoblast differentiation

Mechanical stretch is known to promote osteoblast differentiation in vitro and accelerate bone regeneration in vivo, whereas the relevant mechanism remains unclear. Recent studies have shown the importance of reciprocal interactions between mammalian target of rapamycin (mTOR) and nuclear factor kappa B (NF‐κB; two downstream molecules of Akt) in the regulation of tumor cells. Thus, we hypothesize that mTOR and NF‐κB as well as their interconnection play a critical role in mediating stretch‐induced osteogenic differentiation in osteoblasts. We herein found that mechanical stretch (10% elongation at six cycles/min) significantly promoted the expression of osteoblast differentiation‐related markers (including ALP, BMP2, Col1α, OCN, and Runx2) in osteoblast‐like MG‐63 cells, accompanied by increased mTOR phosphorylation and NF‐κB p65 phosphorylation and nuclear translocation. Blockade of mTOR by antagonist or small interfering RNA suppressed osteogenesis‐related gene expression in response to mechanical stretch, whereas inhibition of NF‐κB further increased stretch‐induced osteoblast differentiation. Moreover, inhibition of mTOR decreased the phosphorylation of NF‐κB, and blockade of NF‐κB reduced the mTOR activation in MG63 cells under mechanical stretch. Coinhibition of mTOR and NF‐κB abolishes the alteration of osteogenic differentiation induced by single mTOR or NF‐κB inhibition under mechanical stretch, which is equivalent to the noninhibition level for osteoblasts under mechanical stretch. The expression levels of osteogenic differentiation in osteoblasts after inhibition of Akt were similar to those after co‐inhibition of mTOR and NF‐κB under mechanical stretch. This study for the first time reveals the reciprocal interconnection between mTOR and NF‐κB in osteoblasts under mechanical stretch and indicates that mTOR and NF‐κB as well as their interactions play a key role in the regulation of cellular homeostasis of osteoblasts in response to mechanical stretch. These findings are helpful for enriching our basic knowledge of the molecular mechanisms of osteoblast mechanotransduction, and also providing insight into the clinical therapeutic modality associated with mechanical stretch (e.g., distraction osteogenesis).
Urological tumors treatment in Brazil during the SARS-Cov-2 outbrake

SARS-CoV-2 pandemic has a great impact in all medical fields and the paper of Anjos Silva and Collegues from Brazil (1) shows interesting data about surgery in patients with urological tumors. Previous studies shows that SARS-CoV-2 changes the urologic practice in several fields (2-5), but the treatment of urological tumors during the outbreak has specially importance (6-8). In this interesting paper the authors studied if uro-oncological surgeries at pandemic are safe in a cohort of 213 patients (161 patients were submitted to elective surgery and 44 to emergency surgery) in a reference hospital in Brazil and concluded that Elective uro-oncological procedures at the COVID-19 epidemic period in a COVID-19-free Institute are safe, and patients who need urgent procedures, with a long period of hospitalization, need special care to avoid COVID-19 infection and its outcomes. We would like to congratulate the authors by the interesting and important report in this paper. EDITORIAL COMMENT Vol. 47 (2): 386-387, March April, 2021 doi: 10.1590/S1677-5538.IBJU. 2020.0479. 1
Krüppel‐like factor 4 (KLF4) directly regulates proliferation in thymocyte development and IL‐17 expression during Th17 differentiation

Krüppel‐like factor 4 (KLF4), a transcription factor, plays a key role in the pluripotency of stem cells. We sought to determine the function of KLF4 in T‐cell development and differentiation by using T‐cell‐specific Klf4‐knockout (KO) mice. We found that KLF4 was highly expressed in thymocytes and mature T cells and was rapidly down‐regulated in mature T cells after activation. In Klf4‐KO mice, we observed a modest reduction of thymocytes (27%) due to the reduced proliferation of double‐negative (DN) thymocytes. We demonstrated that a direct repression of Cdkn1b by KLF4 was a cause of decreased DN proliferation. During in vitro T‐cell differentiation, we observed significant reduction of IL‐17‐expressing CD4+ T cells (Th17; 24%) but not in other types of Th differentiation. The reduction of Th17 cells resulted in a significant attenuation of the severity (35%) of experimental autoimmune encephalomyelitis in vivo in Klf4‐KO mice as compared with the Klf4 wild‐type littermates. Finally, we demonstrated that KLF4 directly binds to the promoter of Il17a and positively regulates its expression. In summary, these findings identify KLF4 as a critical regulator in T‐cell development and Th17 differentiation. —An, J., Golech, S. , Klaewsongkram, J., Zhang, Y. , Subedi, K. , Huston, G. E. , Wood, W. H. , III, Wersto, R. P., Becker, K. G. , Swain, S. L. , Weng, N. Krüppel‐like factor 4 (KLF4) directly regulates proliferation in thymocyte development and IL‐17 expression during Th17 differentiation. FASEB J. 25, 3634–3645 (2011). www.fasebj. org
Transparent number-naming system gives only limited advantage for preschooler's numerical development: Comparisons of Vietnamese and French-speaking children

Several cross-sectional studies have suggested that the transparency of the number-naming system of East Asian languages (Chinese, Japanese) facilitates children's numerical development. The Vietnamese number-naming system also makes the base-10 system very explicit (eleven is "mười một," literally "ten-one," and thirty is "ba mươi," literally "three-ten"). In contrast, Western languages (English, French) include teen words (eleven to sixteen) and ten words (twenty to ninety) that make their counting systems less transparent. The main question addressed in this paper is: To what extent does a language's number-naming system impact preschoolers' numerical development? Our study participants comprised 104 Vietnamese and 104 French-speaking Belgian children between 3½ and 5½ years of age, as well as their parents. We tested the children on eight numerical tasks (counting, advanced counting, enumeration, Give-N, number-word comparison, collection comparison, addition, and approximate addition) and some general cognitive abilities (IQ and phonological loop by letter span). The parents completed a questionnaire on the frequency with which they stimulated their child's numeracy and literacy at home. The results indicated that Vietnamese children outperformed Belgian children only in counting. However, neither group differed in other symbolic or non-symbolic abilities, although Vietnamese parents tended to stimulate their child at home slightly more than Belgian parents. We concluded that the Vietnamese number-naming system's transparency led to faster acquisition of basic counting for preschoolers but did not support other more advanced numerical skills or non-symbolic numerical abilities. In addition, we extended the evidence that both transparent number-naming system and home numeracy influence young children's counting development.
Role of ubiquitylation and USP8‐dependent deubiquitylation in the endocytosis and lysosomal targeting of plasma membrane KCa3.1

We recently demonstrated that plasma membrane KCa3.1 is rapidly endocytosed and targeted for lysosomal degradation via a Rab7‐ and ESCRT‐dependent pathway. Herein, we assess the role of ubiquitylation in this process. Using a biotin ligase acceptor peptide (BLAP)‐tagged KCa3.1, in combination with tandem ubiquitin binding entities (TUBEs), we demonstrate that KCa3.1 is polyubiquitylated following endocytosis. Hypertonic sucrose inhibited KCa3.1 endocytosis and resulted in a significant decrease in channel ubiquitylation. Inhibition of the ubiquitin‐activating enzyme (E1) with UBEI‐41 resulted in reduced KCa3.1 ubiquitylation and internalization. The general deubiquitylase (DUB) inhibitor, PR‐619 attenuated KCa3.1 degradation, indicative of deubiquitylation being required for lysosomal delivery. Using the DUB Chip, a protein microarray containing 35 DUBs, we demonstrate a time‐dependent association between KCa3.1 and USP8 following endocytosis, which was confirmed by coimmunoprecipitation. Further, overexpression of wild‐type USP8 accelerates channel deubiquitylation, while either a catalytically inactive mutant USP8 or siRNA‐mediated knockdown of USP8 enhanced accumulation of ubiquitylated KCa3.1, thereby inhibiting channel degradation. In summary, by combining BLAP‐tagged KCa3.1 with TUBEs and DUB Chip methodologies, we demonstrate that polyubiquitylation mediates the targeting of membrane KCa3.1 to the lysosomes and also that USP8 regulates the rate of KCa3.1 degradation by deubiquitylating KCa3.1 prior to lysosomal delivery. —Balut, C. M. , Loch, C. M. , Devor, D. C. Role of ubiquitylation and USP8‐dependent deubiquitylation in the endocytosis and lysosomal targeting of plasma membrane KCa3.1. FASEB J. 25, 3938–3948 (2011). www.fasebj. org