Miracle-dz/UniFAR-FTUs huggingface dataset:
Coarse-Grained Modeling and Molecular Dynamics Simulations of Ca2+-Calmodulin

Calmodulin (CaM) is a calcium-binding protein that transduces signals to downstream proteins through target binding upon calcium binding in a time-dependent manner. Understanding the target binding process that tunes CaM’s affinity for the calcium ions (Ca2+), or vice versa, may provide insight into how Ca2+-CaM selects its target binding proteins. However, modeling of Ca2+-CaM in molecular simulations is challenging because of the gross structural changes in its central linker regions while the two lobes are relatively rigid due to tight binding of the Ca2+ to the calcium-binding loops where the loop forms a pentagonal bipyramidal coordination geometry with Ca2+. This feature that underlies the reciprocal relation between Ca2+ binding and target binding of CaM, however, has yet to be considered in the structural modeling. Here, we presented a coarse-grained model based on the Associative memory, Water mediated, Structure, and Energy Model (AWSEM) protein force field, to investigate the salient features of CaM. Particularly, we optimized the force field of CaM and that of Ca2+ ions by using its coordination chemistry in the calcium-binding loops to match with experimental observations. We presented a “community model” of CaM that is capable of sampling various conformations of CaM, incorporating various calcium-binding states, and carrying the memory of binding with various targets, which sets the foundation of the reciprocal relation of target binding and Ca2+ binding in future studies.
LEAP-2: An Emerging Endogenous Ghrelin Receptor Antagonist in the Pathophysiology of Obesity

Liver-expressed antimicrobial peptide 2 (LEAP-2), originally described as an antimicrobial peptide, has recently been recognized as an endogenous blocker of growth hormone secretagogue receptor 1a (GHS-R1a). GHS-R1a, also known as ghrelin receptor, is a G protein-coupled receptor (GPCR) widely distributed on the hypothalamus and pituitary gland where it exerts its major functions of regulating appetite and growth hormone (GH) secretion. The activity of GHS-R1a is controlled by two counter-regulatory endogenous ligands: Ghrelin (activation) and LEAP-2 (inhibition). Ghrelin activates GHS-R1a on the neuropeptide Y/Agouti-related protein (NPY/AgRP) neurons at the arcuate nucleus (ARC) to promote appetite, and on the pituitary somatotrophs to stimulate GH release. On the flip side, LEAP-2, acts both as an endogenous competitive antagonist of ghrelin and an inverse agonist of constitutive GHS-R1a activity. Such a biological property of LEAP-2 vigorously blocks ghrelin’s effects on food intake and hormonal secretion. In circulation, LEAP-2 displays an inverse pattern as to ghrelin; it increases with food intake and obesity (positive energy balance), whereas decreases upon fasting and weight loss (negative energy balance). Thus, the LEAP-2/ghrelin molar ratio fluctuates in response to energy status and modulation of this ratio conversely influences energy intake. Inhibiting ghrelin’s activity has shown beneficial effects on obesity in preclinical experiments, which sheds light on LEAP-2’s anti-obesity potential. In this review, we will analyze LEAP-2’s effects from a metabolic point of view with a focus on metabolic hormones (e.g., ghrelin, GH, and insulin), and discuss LEAP-2’s potential as a promising therapeutic target for obesity.
Self-Assembly Engineering Nanodrugs Composed of Paclitaxel and Curcumin for the Combined Treatment of Triple Negative Breast Cancer

The clinical outcomes of triple-negative breast cancer (TNBC) chemotherapy are unsatisfactory. Water solubility and biosafety of chemo drugs are also major barriers for achieving satisfactory treatment effect. In this study, we have reported a combinational strategy by self-assembly engineering nanodrugs PC NDs, which were composed of paclitaxel (PTX) and curcumin (Cur), for effective and safe TNBC chemotherapy. PC NDs were prepared through reprecipitation method without using any additional carriers. The PC NDs were preferentially taken up by TNBC cells and we also observed pH-related drug release. Compared with free PTX and simple PTX/Cur mixture, PC NDs have shown higher therapeutic efficiency and better prognosis while the metastasis rate was significantly lower than that of either PTX or PTX/Cur mix group. Therefore, the self-assembly engineered PC NDs might be a promising nanodrugs for efficient and safe TNBC chemotherapy.
Called to Counsel: Examining the Links Among Calling, Life Meaning, Life Satisfaction, and Burnout Among Law Students

Abstract Adapting the work as calling theory (WCT), the present study used path analysis to examine the relations among calling, person-environment fit (P-E fit), life meaning, living one’s calling, academic burnout, and life satisfaction in a sample of law students (N = 245). Propositions of the WCT model were generally supported by data in which perceiving a calling predicted P-E fit, which in turn predicted life meaning. Life meaning predicted living out one’s calling in law school, which correlated positively with life satisfaction and negatively with academic burnout. Findings highlight the predictive role of calling in law student well-being. Implications for future research and practice are discussed.
Brand Communities on Instagram: Exploring Fortune 500 Companies’ Instagram Communication Practices

ABSTRACT To examine a more holistic communication landscape for companies on Instagram, this study conducted a content analysis of Fortune 500 companies’ official Instagram posts (N = 7,540) using the original three brand community dimensions (i.e., shared consciousness, rituals and traditions, obligations to followers). We also propose and explore a fourth marker, social networking, as an important Instagram communication characteristic for companies and marketers. Findings revealed that while all four brand community practices are observed across eleven industries and 165 companies in our sample, companies did not fully utilize their Instagram accounts to establish strong brand communities. More importantly, there were significant effects of the brand community practices on fans’ behavioral engagement through a number of “likes,” and a number of “views” for video posts. Theoretical and practical implications are addressed.
Correlation Of Rate Of Compliance To A Prescribed Treatment Regimen With Sense Of Power Experienced By Elders With Type Ii Diabetes Mellitus

The purpose of this study was to identify the relation­ ship between elderly diabetics' sense of power and their rate of compliance to a prescribed treatment regimen. The researcher hypothesized that there would be no significant correlation between sense of power and rate of compliance to a prescribed treatment regimen. The Health Care Practice Scale Part A (HCPS-A) and the Health Care Practice ScalePart B (HCPS-B) were administered to 16 Type II diabetics who were 65 years or older. Statistical analysis using Pearson r̂ found no significant relationship ; therefore, the researcher failed to reject the hypothesis. The researcher recommends that a similar study with a larger, more representative sample be conducted. The compliance measurement tool (HCPS-A) should be revised so that it is specific to diabetes. A study comparing diabetics ' perception of the treatment regimen with an a c t u a l p r e scribed treatment regimen should also be conducted. In regards to nursing. Geriatric Nurse Clinicians should encourage compliance and stimulate clients with an external locus of control to accept more responsi­ bility for health maintenance.
Postpartum uterine involution and ovarian follicular dynamics in crossbreed Anatolian water buffalo (Bubalus bubalis) during summer season

Oktay YILMAZ*, Ebubekir YAZICI, Hasan ÇİÇEK, Hacı Ahmet ÇELİK, Cangir UYARLAR, Mehmet UÇAR Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey Department of Animal Health Economics and Management, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
Iron deficiency anemia and its relationship with academic performance in university students

The objective was to know the level of iron deficiency anemia and its relationship that it exerts on the low academic performance of the students at the Professional School of Medical Technology of the Andean University Néstor Cáceres Velásquez de Puno-Peru (2019). The research was quantitative and descriptive, correlational, cross-sectional. The sample consisted of 53 students (women), who were from the I, II, III and IV semesters. To obtain data, the hemoglobin value of the students was determined, and a validated survey was applied to them to know their academic performance. The results showed that there are low percentages of students with iron deficiency anemia. The relationship, according to Spearman Rho, indicates that there is a moderate association between iron deficiency anemia and academic performance.
Dietry Impact of Normal and Germinated Barely Products on body weight and Lipid Profile of Diabetic Rats

The present study aimed to evaluate the effect of barely grains and germinated barely products on lipid profile and lipoprotein of diabetic rats. Male albino rats (n=56) were divided into two main groups, the first group (n=7rats) considered as a control negative group (G1). The remaining 49 rats were injected with Alloxan (120mg/kg BW) for the induction of diabetes, then were divided into (7) subgroups (n=7 rats for each) ,the first subgroup was left without treatment and considered as a control positive group (G2). G3 Diabetic rats were fed on normal barely bread, G 4 Diabetic rats were fed on germinated barely bread, G 5 Diabetic rats were fed on normal barely biscuits, G 6 Diabetic rats were fed on germinated barely biscuits, G7 Diabetic rats were fed on normal barely talbina, G 8 Diabetic rats were fed on germinated barely talbina. After 4 weeks, the mean values of body weight was estimated in all groups. Blood samples were collected to estimate lipid profile including cholesterol, triglycerides, LDL-c, and HDL-c. The results showed that there were a significant loss body weight for rats fed on germinated barely products compared to control possitive. Rats fed on germinated barely products exhibited lower glucose in contrary to insulin and improvement of enzymes activity to level comparable to the normal level. Results also revealed that, feeding rats on germinated barely products such as bread, biscuits and talbina led to significant decrease in serum cholesterol, triglycerides, LDL-c, and increase HDL-c. As conclusion, germinated barely products especially germinated talbina had improved the lipid profile compared with the control. Our results recommended germinated barely could be as functional foods to improve human health.
THE EFFECTIVENESS OF DIGITAL COMICS IN IMPROVING INDUSTRIAL REVOLUTION SKILLS AND STUDENT MOTIVATION

Digital comics are media that support the learning process in the 4.0 era. Digital comics contain the concept of bacteria that is easy for students to understand. The purpose of this study was to analyze the increase in problem-solving skills, critical thinking, and student motivation in learning the concept of bacteria using digital comics. The subjects in this study were 26 third semesters’ students of the biology education program. The comics that are used are digital comics created by researchers using the comic life application. The instruments used were critical thinking test questions and problem-solving and motivation questionnaires. Data analyses were done by using descriptive analysis techniques. The results of the t-test analysis showed that there were differences in students' problem solving and critical thinking abilities before and after learning using digital comics "Bacterial Characteristics" (p <0.05). Meanwhile, student motivation also has differences between the first meeting and the second meeting during learning using digital comics (p <0.05). The use of digital comics can empower students' problem-solving skills, critical thinking, and motivation in learning the concept of bacterial characteristics
Induksi Kalus Morfogenik dan Regenerasi Tanaman Tebu (Saccharum officinarum L.) secara In Vitro Induction of Morphogenic Callus and In Vitro Regeneration of Sugarcane

ABSTRAK Propagasi in vitro merupakan cara memperoleh tanaman baru yang sehat secara cepat dan massal, Percobaan in vitro dilaksanakan untuk menginduksi kalus morfogenik dan regenerasi dari varietas tebu domestik. Bagian terdalam gulungan daun tebu digunakan sebagai eksplan dan diinkubasi pada medium MS yang dimodifikasi dan dilengkapi dengan 2,4-D pada konsentrasi 1 mg/L, 2.5 mg/L, dan 5 mg/L untuk menginduksi pembentukan kalus. Kalus yang terbentuk di medium induksi selanjutnya dipindahkan ke medium MS yang diperkaya dengan zat pengatur tumbuh untuk inisiasi dan perbanyakan tunas. Persentase pembentukan kalus tertinggi dicapai pada penggunaan medium yang mengandung 2.5 mg/L 2,4-D dan maksimal proliferasi kalus morfogenik diperoleh setelah subkultur ketiga. Dua tipe kalus yang terbentuk pada medium induksi yaitu berstruktur kering, remah dan struktur halus kompak. Jenis kalus yang morfogenik tersebut berwarna putih dan putih kekuningan serta mudah terpisah-pisah. Hasil proliferasi tunas dari kalus tertinggi diperoleh pada medium MS dengan kombinasi hormon tumbuh 2 mg/L Kinetin + 1 mg/L IAA dan tidak ada pertumbuhan tunas pada medium yang ditambahkan zat pengatur tumbuh tunggal, yaitu Kinetin. Dengan demikian, penelitian ini menyimpulkan bahwa hormon tumbuh jenis sitokinin yang dikombinasi dengan auksin sangat dibutuhkan untuk regenerasi kalus tebu secara in vitro. Kata Kunci:
Role of Inhibitory Neurosteroid as an Adjunctive Treatment in Refractory Status Epilepticus (Phase IIa Study): Efficacy, Safety, and Bioavailability of Allopregnanolone Converting from Oral Micronized Progesterone

Objectives: To study bioavailability of oral micronized progesterone that convert to allopregnanolone (an inhibitory neurosteroid) and determine its efficacy and safety in treating Thai patients with refractory status epilepticus (RSE). Materials and Methods: A prospective i n te r ven t i ona l s t udy was conduc ted a t Phramongkutklao Hospital. Patients diagnosed with RSE on midazolam infusion were assigned to receive oral progesterone 200 mg every 8 hours via nasogastric tube for 5 days. Standard treatments for RSE were maintained. Serum allopregnanolone levels were monitored. Outcomes including seizure terminations, hospital stays, mortality rates, and accumulated midazolam dosage were analyzed and compared with historical cohort. Results: Total of 6 patients receiving oral micronized progesterone (intervention group), and 6 patients in control group were compared. Demographic data between groups were not different, mean age of 63 and 62.3 years old, respectively. It was shown that oral micronized progesterone clearly converted to allopregnanolone. Allopregnanolone significantly shorten the duration of RSE, 25.5 hours vs. 58.4 hours, p-value 0.004. The average duration of hospital and ICU stays were 57 days, 28.7 days for intervention group and 58.7 days and 44.5 days for control group, p-value 0.929 and 0.399, respectively. The mortality rate of intervention group was lower in intervention group, 33.3% vs. 66.7%, p-value 0.513. The intervention group required lower total accumulated midazolam infusion dosage, 87.5 mg vs. 330.4 mg, p-value 0.394. No any clinical adverse events nor worsening of laboratory profiles were reported. Conclusion: Allopregnanolone, converted from oral micronized progesterone, demonstrated efficacy and safety in treating refractory status epilepticus.
Subgrouping of Type 2 Diabetes Mellitus Patients Based on Proteinuria and Diabetic Control for Treatment and Follow-Up

Patients with type 2 diabetes mellitus (T2DM) exhibit improved symptoms at the start of treatment, but their conditions subsequently deteriorate after a few months of treatment. In the present study, patients treated with glibenclamide and metformin were followed up to evaluate the efficacy of treatment based on some biochemical parameters in T2DM subgroups. The patients with T2DM were subgrouped according to their glycated hemoglobin (HbA1c), sex, urinary albumin to creatinine ratio (UACR), and uric acid UA levels. A total of 662 patients participated in the study, which aimed to follow up with glycated hemoglobin (HbA1c), fasting blood sugar (FBS), uric acid (UA), UACR, and lipid profiles at the beginning of treatment and after 2 and 4 months. Results showed that the best treatment effect occurred when patients were classified according to the UACR and HbA1c. In general, all parameters, especially lipid profile and UA levels, changed when patients exhibit proteinuria compared with those in normal UACR patients. Depending on the glycemic control subgroups, all parameters were higher in patients with poor glycemic control than those with fair glycemic control, except for HDLc, which was lowest in the poor-control patients. The parameters revealed significant improvement (p<0.05) in lipids, UA, UACR, and HbA1c after 2 months of treatment but subsequently remained slightly increased after 4 months. The parameters measured by using subgroups were better than those obtained from the whole group. The patients who initially experienced improved symptoms tended to ignore regular treatment and restricted diet, thereby worsening the measured parameters.
Development of Pascal Principle Activities Using The Stem Approach and Undergraduate Physics Students’ Perception Towards The Activities

The aim of this study was to develop Pascal Principle teaching and learning activities using the STEM approach and to determine the content validity and undergraduate Physics students’ perception of the activities. This study was a developmental research using ADDIE (Analysis, Design, Development, Implementation, and Evaluation) model to develop the activities. The population was 135 physics undergraduates in their fifth and seventh semester of study from one of the public universities in Malaysia. Data was collected using a questionnaire that was adapted from a standardised instrument and that was distributed using Google Form to the population together with a link to the activities. The response rate was 29.6% (40 respondents). Perception data were analysed descriptively using percentage for content, language, design, and interest. Result shows that the experts were 95.5% in agreement about the content validity of the activities. For the perception data, 98.5%, 99.5%, and 99.0% students have positive perceptions towards the content, language, and design, respectively. In addition, 96.3% show interest in the activities. In conclusion, the research succeeded in developing validated teaching and learning activities that use the STEM approach and that were perceived positively by physics undergraduates. The materials for the activities can thus be used as a teaching and learning resource in the classroom.
Utilization of melamine impregnated paper waste as a filler in thermoplastic composites

The potential utilization of melamine impregnated paper (MIP) waste in thermoplastic composites was investigated. Composites were also manufactured utilizing wood flour (WF) at the same filler rates for comparison. The composites were manufactured using a compression molding method. The effects of filler type and filler rate on the mechanical properties of low-density polyethylene (LDPE)-based composites were evaluated. Mechanical properties, such as tensile and flexural strengths, were determined in accordance with ASTM D638 (2001) and ASTM D790 (2003), respectively. Results showed that filler type and filler content had significant effects on all mechanical properties investigated. Both fillers improved all mechanical properties except for tensile strength and elongation at break of LDPE. In conclusion, MIP waste has a potential to be utilized in thermoplastic-based composite manufacturing and might generate some economic and environmental benefits.
Effect Of Investment Decision, Capital Structure, Profitability, And Company Size On Company Values

This study wanted to examine the effect of investment decisions, capital structure, profitability, and company size on the value of the firm, by using samples from the property, real estate, and construction companies listed on the Indonesia stock exchange for the period 2014-2017. Hypothesis testing using the panel data method by using Eviews version 9. The results of the study showed that the investment decision, capital structure, and profitability did not have a significant effect on firm value. In contrast, the size of the company has a significant adverse effect on the value of the firm. From the results of the F test, investment decision, capital structure, profitability, company size in this study simultaneously influence the value of the firm.
The Potential of Using Kahoot to Empower EFL Learners : An Experience of Game-based Learning

These days, educationists and students are growing enthusiasm for digital learning which is based on pedagogical games. A review of the body of research confirms that these online instructional games are efficient at getting the learners involved in the learning process. The present research investigates using an online educational game-based platform called Kahoot to enhance learning English for Academic Purposes (EAP) and sustains the hypothesis that it enriches the traditional, teacher-centered classrooms by having EFL learners more engaged, motivated, and involved. Applying a mixed-method study, around 100 EFL undergraduates were surveyed and evaluated based on a semester-long involvement in Kahoot games in an EAP course. The subjects were interviewed for their perceptions of using Kahoot and also evaluated statistically by their performance on the achievement test of the course. The findings of the project confirm an enhancement of the EFL learners' language skill performance. Furthermore, Kahoot helped EFL learners experience a high degree of engagement in comparison with traditional teaching methods. Besides, new horizons toward the future of application of Kahoot in EFL learning are discussed. Consequently, both parts of the study i.e., qualitative and quantitative, approved that playing Kahoots, as a platform for game-based learning, was highly engaging, educationally helpful, and motivating.
Investigation of the Effects of Inhibiting N-glycosylation in Cancer

Glycosylation, the addition of sugar moieties to nascent proteins, is one of the most common posttranslational modifications. Glycosylation regulates protein structure, function and localization. Most cell surface proteins and secreted proteins are glycosylated by the addition of Asparagine(N)-linked glycans (N-glycans). Aberrant Nglycosylation is a well-accepted feature of malignancy and is a potential prognostic marker for some types of cancer. For example, increased expression of complex Nglycans has been detected in cancers of breast, colon and has been correlated with reduced survival of the patients. Therefore, understanding the role of N-glycosylation in malignancy could be beneficial for developing novel therapeutic and prognostic strategies. To examine the role of N-glycosylation in malignancy, we applied chemical biology and genetic approaches. First, we conducted a high throughput screen to identify compounds that could block L-PHA-induced cell death. Our screen identified the cardiac glycoside Na + /K + -ATPase inhibitors as novel inhibitors of N-glycosylation. Further analysis of N-glycans consistently confirmed that inhibition of Na + /K + -ATPase impairs the N-glycosylation, as well as migration and invasion. Interestingly, other studies have shown antimetastatic effects of cardiac glycosides in patients. Thus, our high throughput