Miracle-dz/UniFAR-FTUs huggingface dataset:
Stress and headaches in university students during the COVID-19 pandemic

With regards to the relationship between mental and physical health conditions, some studies predict increased headache frequency as a result of exposure to stressful situations. Thus, the purpose of our study was to investigate whether headache characteristics among a sample of university students (N = 234) correlated with fear of COVID-19, stress and quality of life vis-à-vis the pandemic. We found statistically significant positive correlations between headache frequency and both general stress and quality of life. Further, results from a multiple regression analysis suggested that fear of COVID-19 did not account for incremental variance in headache intensity. Explanations for our key findings, limitations of our study, and future directions for post pandemic studies on headache experience are outlined.
Crustal Processes: Major Controls on Reykjanes Peninsula Lava Chemistry, SW Iceland

janes Peninsula, SW Iceland, provide the basis for this study. This basaltsarea is an elevated section of mid-ocean ridge inﬂuenced by theIceland Plume. Selected chemical, Sr, Nd and laser-assisted ﬂuo-rination oxygen isotope data are presented. The dataset is subdividedinto groups based on criteria which are independent of degree of
DNA superhelicity

Abstract Closing each strand of a DNA duplex upon itself fixes its linking number L. This topological condition couples together the secondary and tertiary structures of the resulting ccDNA topoisomer, a constraint that is not present in otherwise identical nicked or linear DNAs. Fixing L has a range of structural, energetic and functional consequences. Here we consider how L having different integer values (that is, different superhelicities) affects ccDNA molecules. The approaches used are primarily theoretical, and are developed from a historical perspective. In brief, processes that either relax or increase superhelicity, or repartition what is there, may either release or require free energy. The energies involved can be substantial, sufficient to influence many events, directly or indirectly. Here two examples are developed. The changes of unconstrained superhelicity that occur during nucleosome attachment and release are examined. And a simple theoretical model of superhelically driven DNA structural transitions is described that calculates equilibrium distributions for populations of identical topoisomers. This model is used to examine how these distributions change with superhelicity and other factors, and applied to analyze several situations of biological interest.
Excess phosphoserine-129 α-synuclein induces synaptic vesicle trafficking and declustering defects at a vertebrate synapse

α-Synuclein is a presynaptic protein that regulates synaptic vesicle (SV) trafficking. In Parkinson’s disease (PD) and dementia with Lewy bodies (DLB), α-synuclein aberrantly accumulates throughout neurons, including at synapses. During neuronal activity, α-synuclein is reversibly phosphorylated at serine 129 (pS129). While pS129 comprises ∼4% of total α-synuclein under physiological conditions, it dramatically increases in PD and DLB brains. The impacts of excess pS129 on synaptic function are currently unknown. We show here that compared with wild-type (WT) α-synuclein, pS129 exhibits increased binding and oligomerization on synaptic membranes and enhanced vesicle “microclustering” in vitro. Moreover, when acutely injected into lamprey reticulospinal axons, excess pS129 α-synuclein robustly localized to synapses and disrupted SV trafficking in an activity-dependent manner, as assessed by ultrastructural analysis. Specifically, pS129 caused a declustering and dispersion of SVs away from the synaptic vicinity, leading to a significant loss of total synaptic membrane. Live imaging further revealed altered SV cycling, as well as microclusters of recently endocytosed SVs moving away from synapses. Thus, excess pS129 caused an activity-dependent inhibition of SV trafficking via altered vesicle clustering/reclustering. This work suggests that accumulation of pS129 at synapses in diseases like PD and DLB could have profound effects on SV dynamics.
MCSfM: Multi-Camera-Based Incremental Structure-From-Motion

Fully perceiving the surrounding world is a vital capability for autonomous robots. To achieve this goal, a multi-camera system is usually equipped on the data collecting platform and the structure from motion (SfM) technology is used for scene reconstruction. However, although incremental SfM achieves high-precision modeling, it is inefficient and prone to scene drift in large-scale reconstruction tasks. In this paper, we propose a tailored incremental SfM framework for multi-camera systems, where the internal relative poses between cameras can not only be calibrated automatically but also serve as an additional constraint to improve the system robustness. Previous multi-camera based modeling work has mainly focused on stereo setups or multi-camera systems with known calibration information, but we allow arbitrary configurations and only require images as input. First, one camera is selected as the reference camera, and the other cameras in the multi-camera system are denoted as non-reference cameras. Based on the pose relationship between the reference and non-reference camera, the non-reference camera pose can be derived from the reference camera pose and internal relative poses. Then, a two-stage multi-camera based camera registration module is proposed, where the internal relative poses are computed first by local motion averaging, and then the rigid units are registered incrementally. Finally, a multi-camera based bundle adjustment is put forth to iteratively refine the reference camera and the internal relative poses. Experiments demonstrate that our system achieves higher accuracy and robustness on benchmark data compared to the state-of-the-art SfM and SLAM (simultaneous localization and mapping) methods.
Lamb weather types derived from reanalysis products

Weather typing, based on surface pressure charts, has been one of the principal means of analysis in synoptic climatology. Here, we summarize the history of manual and automated schemes, in the northwest European context, illustrating how the approaches can take advantage of the extended reanalysis products that have recently become available. The British Isles and the associated Lamb weather types (LWTs) are the focus of this study, but the approach can be applied to any mid‐to‐high latitude region to provide series back to 1871. However, caution is advised in the use of the approach where the quality and quantity of input data into the extended reanalysis are known to be temporally and spatially variable and/or poor in early years. This study intercompares the use of automated schemes with operational analyses, reanalyses and earlier manually derived LWTs. Copyright © 2012 Royal Meteorological Society
Association of Diabetes Mellitus in Pregnancy and Perinatal Depression

ABSTRACT Objective Diabetes is frequently linked with depression, and both conditions are common complications during pregnancy. However, research findings exploring the relationship between diabetes mellitus in pregnancy (DMP) and perinatal depression (PND) have been inconsistent. Thus, this study seeks to examine the association between DMP and PND in a prospective population-based cohort. Methods Women aged 18 to 48 years (n = 4459) were identified from the Biology, Affect, Stress, Imaging and Cognition study. The diagnosis of DMP was based on International Classification of Diseases code O24 from medical records and was classified as pregestational, gestational, or unspecified diabetes. PND was assessed using psychometric instruments, clinical interviews, and/or register data and categorized into antepartum or postpartum depression. Multivariable logistic regressions were used to study the associations of DMP with antepartum and postpartum depression. The association between DMP and continuous depression scores, antepartum and postpartum, was investigated with multivariable linear regressions. Results Of 4459 pregnancies, 949 women had antepartum depression (21.2%) and 1123 had postpartum depression (25%). DMP had a prevalence of 1.2%. Women with DMP had twofold higher odds for postpartum depression compared with women without DMP. Although no association was observed between DMP and antepartum depression, DMP was associated with higher antepartum depression scores. Conclusions Our study shows an association between DMP and PND, which might be considered a risk factor when screening for high-risk groups.
Petrogenesis of Neogene potassic vol can ic rocks in the Pannonian Basin

Ci J ~, We present a description of the raTe Neogene potassiclultrapotassic rocks in the Pannonian Basin. The Middie Miocene Balatonmaria (BM) volcanics were revealed by drilling cores in the central part of the Pannonian Basin. They are silica-saturated and can be classified into the orogenic Group III of ultrapotassic rocks. Mineral chemical data and trace element geochemistry indicate a subduction-related character. Geochemical similarity between the BM potassic rocks and the Gleichenberg (GLE) latite (Graz Basin) is remarkable. The Late Pliocene leucitite of Bar (southern Pannonian Basin) is silica-undersaturated and has a transitional character between Group III ultrapotassic rocks and lamproites. High mg# and high Ff SE contents could imply a primitive composition. It has a strongly fractionated REE pattern, a weak Nb-trough and a positive Zr-Hf anomaly in a primitive mantle-normalized trace element variation diagram. AlI of these suggest a different origin from the BM and GLE potassic rocks. Eruption of the BM and GLE potassic magmas can be related to the Middie Miocene extensional phase of the Pannonian Basin. Trace element geochemistry of the BM and GLE rocks is consistent with 2-component mixing process between a magma derived from a moderately enriched mantle source and a sedimentary component. The LILE and particularly the K-enrichment could be related to the Late Eocene southward subduction of the oceaniclattenuated continentallithosphere of the Magura Basin. On the other hand, the Bar leu~itite involves mixing between asthenospheric magma and melts from the lower lithosphere consisting of K-rich metasomatic layers. Generation of the ultrapotassicmagma could be attributed to the Pliocene (Late Pannonian) renewal of transtensional strike-slip fault activity or to a local asthenospheric mantle upwelling heating the lower lithospheric mantle.
Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2

Abstract Post-translational modifications (PTMs) of histones have fundamental effects on chromatin structure and function. While the impact of PTMs on the function of core histones are increasingly well understood, this is much less the case for modifications of linker histone H1, which is at least in part due to a lack of proper tools. In this work, we establish the assembly of intact chromatosomes containing site-specifically ubiquitylated and acetylated linker histone H1.2 variants obtained by a combination of chemical biology approaches. We then use these complexes in a tailored affinity enrichment mass spectrometry workflow to identify and comprehensively characterize chromatosome-specific cellular interactomes and the impact of site-specific linker histone modifications on a proteome-wide scale. We validate and benchmark our approach by western-blotting and by confirming the involvement of chromatin-bound H1.2 in the recruitment of proteins involved in DNA double-strand break repair using an in vitro ligation assay. We relate our data to previous work and in particular compare it to data on modification-specific interaction partners of free H1. Taken together, our data supports the role of chromatin-bound H1 as a regulatory protein with distinct functions beyond DNA compaction and constitutes an important resource for future investigations of histone epigenetic modifications.
Why are so many enteric pathogen infections asymptomatic? Pathogen and gut microbiome characteristics associated with diarrhea symptoms and carriage of diarrheagenic E. coli in northern Ecuador

ABSTRACT A high proportion of enteric infections, including those caused by diarrheagenic Escherichia coli (DEC), are asymptomatic for diarrhea. The factors responsible for the development of diarrhea symptoms, or lack thereof, remain unclear. Here, we used DEC isolate genome and whole stool microbiome data from a case–control study of diarrhea in Ecuador to examine factors associated with diarrhea symptoms accompanying DEC carriage. We investigated i) pathogen abundance, ii) gut microbiome characteristics, and iii) strain-level pathogen characteristics from DEC infections with diarrhea symptoms (symptomatic infections) and without diarrhea symptoms (asymptomatic infections). We also included data from individuals with and without diarrhea who were not infected with DEC (uninfected cases and controls). i) E. coli relative abundance in the gut microbiome was highly variable, but higher on-average in individuals with symptomatic compared to asymptomatic DEC infections. Similarly, the number and relative abundances of virulence genes in the gut were higher in symptomatic than asymptomatic DEC infections. ii) Measures of microbiome diversity were similar regardless of diarrhea symptoms or DEC carriage. Proteobacterial families that have been described as pathobionts were enriched in symptomatic infections and uninfected cases, whereas potentially beneficial taxa, including the Bacteroidaceae and Bifidobacteriaceae, were more abundant in individuals without diarrhea. An analysis of high-level gene functions recovered in metagenomes revealed that genes that were differentially abundant by diarrhea and DEC infection status were more abundant in symptomatic than asymptomatic DEC infections. iii) DEC isolates from symptomatic versus asymptomatic individuals showed no significant differences in virulence or accessory gene content, and there was no phylogenetic signal associated with diarrhea symptoms. Together, these data suggest signals that distinguish symptomatic from asymptomatic DEC infections. In particular, the abundance of E. coli, the virulence gene content of the gut microbiome, and the taxa present in the gut microbiome have an apparent role.
A comprehensive study of the interactions in the B. subtilis degradosome with special emphasis on the role of the small proteins SR1P and SR7P

Here, we employ coelution experiments and far‐western blotting to identify stable interactions between the main components of the B. subtilis degradosome and the small proteins SR1P and SR7P. Our data indicate that B. subtilis has a degradosome comprising at least RNases Y and PnpA, enolase, phosphofructokinase, glycerol‐3‐phosphate dehydrogenase GapA, and helicase CshA that can be co‐purified without cross‐linking. All interactions were corroborated by far‐western blotting with proteins purified from E. coli. Previously, we discovered that stress‐induced SR7P binds enolase to enhance its interaction with and activity of enolase‐bound RNase Y (RnY), while SR1P transcribed under gluconeogenic conditions interacts with GapA to stimulate its interaction with and the activity of RnjA (RnjA). We show that SR1P can directly bind RnjA, RnY, and PnpA independently of GapA, whereas SR7P only interacts with enolase. Northern blotting suggests that the degradation of individual RNAs in B. subtilis under gluconeogenic or stress conditions depends on either RnjA or RnY alone or on RnjA‐SR1P, RnY‐SR1P, or RnY‐Eno. In vitro degradation assays with RnY or RnjA substrates corroborate the in vivo role of SR1P. Currently, it is unknown which substrate property is decisive for the utilization of one of the complexes.
Effects of Cognitive Training on Alzheimer’s Disease and Related Dementias: The Moderating Role of Social Determinants of Health

Objective: We examined whether social determinants of health (SDoH) are associated with Alzheimer’s disease and related dementias (ADRD) risk and the effects of cognitive training over a 20-year follow-up period. Methods: Data were obtained from 1605 participants in ACTIVE. SDoH measures were created using baseline data at the individual and neighborhood level. Incident ADRD was defined using administrative claims data (1999–2019). Cause-specific hazard models estimated associations between SDoH and claims-based diagnosed ADRD. Results: Higher scores on neighborhood and built environment were associated with lower ADRD risk. Trained participants obtained a greater degree of protection from ADRD when they had higher scores for SDoH domains associated with health care and education access. However, there were fewer significant SDoH moderation effects on cognitive training than expected. Discussion: Future work should continue to explore culturally tailored cognitive training interventions to reduce ADRD risk associated with SDoH that disproportionately affects racially diverse aging populations.
Discharges against medical advice: time to take another look. A retrospective review of discharges against medical advice focused on prevention

ABSTRACT Background Discharges against medical advice (DAMA) increase the risk of death. Methods We retrieved DAMA from five hospitals within a large health system and reviewed 10% of DAMA from the academic site between 2016 and 2021. Results DAMA increased at the onset of the pandemic. Patients who discharged AMA multiple times accounted for a third of all DAMA. Detailed review was completed for 278 patients who discharged AMA from the academic site. In this sample, women comprised 52% of those who discharged AMA multiple times. Relative to the proportion of all discharges from the academic site during the study period, Black patients were overrepresented among DAMA (21% vs. 34%, p < .05). Patients with multiple AMA discharges were younger, more likely to be unmarried, or have substance use disorders (SUD) than those who discharged AMA once. The most common reason for requesting premature discharge noted in n = 77, 28% of instances was related to patient obligations outside the hospital. Hospital policies and procedures contributed in n = 29, 10% of instances. Reasons for requesting premature discharge and documentation of key safety processes were similar by gender and race however the sample may be underpowered to detect differences. Capacity was evaluated in 109 (39%). Among those who consumed alcohol (n = 81 (29%)) or had SUDs (n = 112 (40%)), information on the amount or timing of last use was missing in n = 39 (48%) and n = 74 (66%), respectively. Critical tools to manage illness were provided in 45 (16%) of DAMA reviewed. Conclusions Drivers of AMA discharge may differ by AMA discharge frequency. Recognition of the common reasons for requesting premature discharge may help destigmatize AMA discharges and also identifies early assessments by social work colleagues as an important prevention strategy. Opportunities also exist in anticipating and preventing withdrawal symptoms and in revising hospital practices that contribute to DAMA.
P2X7 accelerate tissue fibrosis via metalloproteinase 8‐dependent macrophage infiltration in a murine model of unilateral ureteral obstruction

Renal fibrosis is tightly associated with chronic kidney disease, irrespective of the underlying pathogenesis. We previously demonstrated mild antifibrotic effects of targeting the P2X7 receptor in a pyelonephritis model. Reduced P2X7R‐activation elevated the neutrophil‐to‐macrophage ratio, resulting in less matrix accumulation without affecting the initial tissue healing. Here, we test if this P2X7R‐dependent modification of matrix accumulation also applies to a noninfectious fibrosis model of unilateral ureteral obstruction (7dUUO) and whether the response is gender‐dependent. We found that P2X7−/− mice show reduced fibrosis compared to wild type after 7dUUO: the effect was most pronounced in females, with a 55% decrease in collagen deposition after 7dUUO (p < 0.0068). P2X7R deficiency did not affect early fibrosis markers (TGF‐β, α‐SMA) or the renal infiltration of neutrophils. However, a UUO‐induced increase in macrophages was observed in wildtypes only (p < 0.001), leaving the P2X7−/− mice with ≈50% fewer CD68+ cells in the renal cortex (p = 0.018). In males, 7dUUO triggered an increase in diffusely interstitial scattering of the profibrotic, macrophage‐attracting metalloproteinase MMP8 and showed significantly lower MMP8 tissue expression in both male and female P2X7−/− mice (p < 0.0008). Thus, the P2X7R is advocated as a late‐stage fibrosis moderator by reducing neutrophil‐dependent interstitial MMP8 release, resulting in less macrophage infiltration and reduced matrix accumulation.
Modelling Geomorphic Systems: Fluvial

Rivers and floodplains convey and exchange water and all its constituent matter from Earth's surface to an intra-continental or oceanic sink. The associated processes of flow and flux are the foundation of all ecosystem service provision and human value derived from river environments. Numerical modelling is one of many approaches that may be used to understand these processes. It is an approach that seeks quantitative mechanistic understanding that is critical to enhancing the predictive capacity of river science, and to developing evidence-based management practices. In combination with other approaches, modelling should play a key role in constraining understanding of river responses in an uncertain future. Ongoing improvements in computing power and in the availability and accessibility of fluvial modelling codes have substantially increased the uptake of modelling as a method of investigating fluvial processes and forms. This chapter outlines the physical basis of different types of fluvial model, and illustrates the key considerations needed to select a model code with the necessary numerical complexity, to establish a physical model domain and boundary conditions, to test for sensitivity to domain, boundary and parameter variables, and to evaluate results.
Breastfeeding and less healthy beverage intake during the first year of life

Breastfeeding during infancy is associated with healthier beverage consumption later in childhood, but little is known about this relation during infancy. This was a longitudinal study of breastfeeding and less healthy beverage consumption during the first year of life, in a birth cohort study conducted 2013–2018 in the Southeastern United States (n = 666).