Miracle-dz/UniFAR-FTUs huggingface dataset:
A Mediterranean Alexandrium taylorii (Dinophyceae) Strain Produces Goniodomin A and Lytic Compounds but Not Paralytic Shellfish Toxins

Species of the dinophyte genus Alexandrium are widely distributed and are notorious bloom formers and producers of various potent phycotoxins. The species Alexandrium taylorii is known to form recurrent and dense blooms in the Mediterranean, but its toxin production potential is poorly studied. Here we investigated toxin production potential of a Mediterranean A. taylorii clonal strain by combining state-of-the-art screening for various toxins known to be produced within Alexandrium with a sound morphological and molecular designation of the studied strain. As shown by a detailed thecal plate analysis, morphology of the A. taylorii strain AY7T from the Adriatic Sea conformed with the original species description. Moreover, newly obtained Large Subunit (LSU) and Internal Transcribed Spacers (ITS) rDNA sequences perfectly matched with the majority of other Mediterranean A. taylorii strains from the databases. Based on both ion pair chromatography coupled to post-column derivatization and fluorescence detection (LC-FLD) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis it is shown that A. taylorii AY7T does not produce paralytic shellfish toxins (PST) above a detection limit of ca. 1 fg cell−1, and also lacks any traces of spirolides and gymnodimines. The strain caused cell lysis of protistan species due to poorly characterized lytic compounds, with a density of 185 cells mL−1 causing 50% cell lysis of cryptophyte bioassay target cells (EC50). As shown here for the first time A. taylorii AY7T produced goniodomin A (GDA) at a cellular level of 11.7 pg cell−1. This first report of goniodomin (GD) production of A. taylorii supports the close evolutionary relationship of A. taylorii to other identified GD-producing Alexandrium species. As GD have been causatively linked to fish kills, future studies of Mediterranean A. taylorii blooms should include analysis of GD and should draw attention to potential links to fish kills or other environmental damage.
Effect of integrated nutrient management on growth and yield of green gram (Vigna radiata L.)

A field experiment was conducted during Kharif season 2014 at the Research farm of Soil Science, Allahabad School of Agriculture, laid out in randomized block design, consisted nine treatments and three replications, it was observed that growth and yield of green gram in treatment N20P40K40+ FYM @10 t ha-1 and Rhizobium was maximum. Maximum plant height 50.66 cm, number of leaves plant-1 33.00, number of branches plant-1 4.66 at 60 DAS, number of cluster plant-1 9.33, number of pods plant-1 37.33 and total seed yield 12.10 q ha-1 were found to be significant over all other treatment. Adequate plant nutrient supply holds the key for improving the growth and food grain production of crop.
Conformational dependence of integrin‐binding peptides derived from homologous loop regions in the laminin α chains

Laminin α chains (α1–α5 chains) are expressed in a tissue‐ and developmental stage‐specific manner and have diverse chain‐specific biological functions. Especially, laminin globular (LG) modules (LG1–LG5) located at the C‐terminus of the α chains play a critical role in the biological activities of laminins. Each LG module is composed of a 14‐stranded β‐sheet (A‐N) sandwich structure. We previously screened cell attachment activity of the loop regions between the E and F strands in the LG modules using 17 homologous peptides (EF peptides) and found that four active EF peptides bind to integrin α2β1. One of the four peptides, G4EF1 demonstrated improved cell attachment activity when cyclized. Here, we focused on the remaining three integrin α2β1‐binding EF peptides (G5EF1, G3EF3, and G5EF5) and analyzed the relationship between their peptide conformation and cell attachment activity. First, we determined their active core sequences and found that G5EF1z (IGLEIVDGKVLFHVNN), G3EF3z (LLVTLEDGHIALST), and G5EF5z (KVLTEQVL) are the core sequences. Cyclic peptides of the core sequences (cycloG5EF1z, cycloG3EF3z, and cycloG5EF5z) enhanced integrin‐mediated cell adhesion activity compared with their linear peptides. The results indicated that cell adhesion activity of the integrin α2β1‐binding EF peptides is conformation dependent and that the loop structure is critical for their activity. This suggests that conformation of the loop regions plays an important role for the activities of the LG modules.
Incidence and risk factors of oral feeding intolerance in acute pancreatitis: Results from an international, multicenter, prospective cohort study

Abstract Background Inability to advance to an oral diet, or oral feeding intolerance, is a common complication in patients with acute pancreatitis associated with worse clinical outcomes. The factors related to oral feeding intolerance are not well studied. Objective We aimed to determine the incidence and risk factors of oral feeding intolerance in acute pancreatitis. Methods Patients were prospectively enrolled in the Acute Pancreatitis Patient Registry to Examine Novel Therapies in Clinical Experience, an international acute pancreatitis registry, between 2015 and 2018. Oral feeding intolerance was defined as worsening abdominal pain and/or vomiting after resumption of oral diet. The timing of the initial feeding attempt was stratified based on the day of hospitalization. Multivariable logistic regression was performed to assess for independent risk factors/predictors of oral feeding intolerance. Results Of 1233 acute pancreatitis patients included in the study, 160 (13%) experienced oral feeding intolerance. The incidence of oral feeding intolerance was similar irrespective of the timing of the initial feeding attempt relative to hospital admission day (p = 0.41). Patients with oral feeding intolerance were more likely to be younger (45 vs. 50 years of age), men (61% vs. 49%), and active alcohol users (44% vs. 36%). They also had higher blood urea nitrogen (20 vs. 15 mg/dl; p < 0.001) and hematocrit levels (41.7% vs. 40.5%; p = 0.017) on admission; were more likely to have a nonbiliary acute pancreatitis etiology (69% vs. 51%), systemic inflammatory response syndrome of 2 or greater on admission (49% vs. 35%) and at 48 h (50% vs. 26%), develop pancreatic necrosis (29% vs. 13%), moderate to severe acute pancreatitis (41% vs. 24%), and have a longer hospital stay (10 vs. 6 days; all p < 0.04). The adjusted analysis showed that systemic inflammatory response syndrome of 2 or greater at 48 h (odds ratio 3.10; 95% confidence interval 1.83–5.25) and a nonbiliary acute pancreatitis etiology (odds ratio 1.65; 95% confidence interval 1.01–2.69) were independent risk factors for oral feeding intolerance. Conclusion Oral feeding intolerance occurs in 13% of acute pancreatitis patients and is independently associated with systemic inflammatory response syndrome at 48 h and a nonbiliary etiology.
Evaluation of the breast cancer care network within the Lazio Region (Central Italy)

Background and objectives A summary indicator for evaluating the breast cancer network has never been measured at the regional level. The aim is to design treemaps providing a summary description of hospitals (including breast units) and Local Health Units (LHUs) in terms of their levels of performance within the breast cancer network of the Lazio region (central Italy). The treemap structure has an intuitive design and displays information from both general and specific analyses. Methods Patients admitted to the regional hospitals for malignant breast cancer (MBC) surgery in 2010–2017 were selected in a population-based cohort study. These quality indicators were calculated based on the international guidelines (EUSOMA, ESMO) to assess the performance in terms of volume of activity, surgery procedure, post-surgery assistance and timeliness of medical therapy or radiotherapy beginning. The quality indicators were calculated using administrative health data systematically collected at the regional level and were included in the treemap to represent the surgery or the post-surgery areas of the breast cancer clinical pathway. In order to allow aggregation of scores for different indicators belonging to the same clinical area, up to five evaluation classes were defined using the "Jenks Natural Breaks" algorithm. A score and a colour were assigned to each clinical area based on the ranking of the indicators involved. The analyses were performed on an annual basis, by the LHU of residence and by the hospital which performed the surgical intervention. Results In 2017, 6218 surgical interventions for MBC were performed in the hospitals of Lazio. The results showed a continuous increase of the level of performance over the years. Hospitals showed higher variability in the levels of performance than the LHUs. 36% of the evaluated hospitals reached a high level of performance. An audit of the S. Filippo Neri breast unit revealed incorrect coding of the input data. For this reason, the score for the indicator for the volume of wards was re-calculated and re-evaluated, with a subsequent improvement of the level of performance. Most LHUs achieved at least an average overall level of performance, with 20% of the LHUs reaching a high level of performance. Conclusions This is the first attempt to apply the treemap logic to a single clinical network, in order to obtain a summary indicator for the evaluation of the breast cancer care network. Our results supply decision makers with a transparent instrument of governance for heterogeneous users, directing efforts improving and promoting equity of care. The treemaps could be reproduced and adapted for other local contexts, in order to limit inappropriateness and ensure uniform levels of breast cancer care within local areas. The next step is the evaluation of audit and feedback interventions to improve the quality of care and to guarantee homogeneous levels of care throughout the region.
Dual Learning for Facial Action Unit Detection Under Nonfull Annotation

Most methods for facial action unit (AU) recognition typically require training images that are fully AU labeled. Manual AU annotation is time intensive. To alleviate this, we propose a novel dual learning framework and apply it to AU detection under two scenarios, that is, semisupervised AU detection with partially AU-labeled and fully expression-labeled samples, and weakly supervised AU detection with fully expression-labeled samples alone. We leverage two forms of auxiliary information. The first is the probabilistic duality between the AU detection task and its dual task, in this case, the face synthesis task given AU labels. We also take advantage of the dependencies among multiple AUs, the dependencies between expression and AUs, and the dependencies between facial features and AUs. Specifically, the proposed method consists of a classifier, an image generator, and a discriminator. The classifier and generator yield face–AU–expression tuples, which are forced to coverage of the ground-truth distribution. This joint distribution also includes three kinds of inherent dependencies: 1) the dependencies among multiple AUs; 2) the dependencies between expression and AUs; and 3) the dependencies between facial features and AUs. We reconstruct the inputted face and AU labels and introduce two reconstruction losses. In a semisupervised scenario, the supervised loss is also incorporated into the full objective for AU-labeled samples. In a weakly supervised scenario, we generate pseudo paired data according to the domain knowledge about expression and AUs. Semisupervised and weakly supervised experiments on three widely used datasets demonstrate the superiority of the proposed method for AU detection and facial synthesis tasks over current works.
Pemafibrate Dramatically Ameliorated the Values of Liver Function Tests and Fibrosis Marker in Patients with Non-Alcoholic Fatty Liver Disease.

Background Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease related to metabolic syndrome, which can progress to liver cirrhosis. Standard medication has not been established. Pemafibrate is a selective peroxisome proliferator-activated receptor (PPAR) α modulator. We retrospectively evaluated the efficacy of pemafibrate in patients with NAFLD. Methods We retrospectively enrolled 17 patients (ten men, seven women; median age, 63 years; range, 27-81 years). They were all proven to have fatty liver through imaging and had little or no history of drinking (ethanol consumption of < 20 g/day for women and < 30 g/day for men). They were administered pemafibrate from October 2018 to June 2020. Results After administration, serum triglyceride (TG) tended to be decreased (300.5 ± 22.5 to 239.5 ± 34.3 mg/dL, P = 0.06). Serum high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL) cholesterol levels did not change. ALT was significantly decreased (-47.4%) for six months (57.5 ± 8.8 to 30.3 ± 5.8 U/L, P < 0.01). The values of serum GGT significantly decreased (-48.7%) for sixth months (63.9 ± 10.3 to 32.8 ± 6.6 U/L, P < 0.01). Aspartate aminotransferase (AST) to platelet ratio (APRI), a fibrosis marker, also was significantly decreased in the sixth month (0.7 ± 0.1 to 0.4 ± 0.1, P < 0.05). Body mass index (BMI) and hemoglobin A1c (HbA1c) showed no significant change. Conclusion Pemafibrate dramatically ameliorated the values of liver function tests and APRI in patients with NAFLD.
Pollen limitation in a monocarpic species, Ipomopsis aggregata

(1) We tested whether seed production in the perennial monocarpic plant Ipomopsis aggregata (Polemoniaceae) was pollen-limited by adding outcross pollen to open stigmas of plants on three occasions at weekly intervals. We compared fruit set and seed set per fruit with a set of adjacent, untreated plants of the same average height and flower number. (2) Experimental pollination doubled the number of flowers producing seed after each occasion and increased the average seed set per fruit for the first one. (3) Plants treated with pollen showed correlations between seed production and height and aborted seed production consistent with resource limitation, but untreated, naturally pollinated plants did not.
Demonstration gardens improve agricultural production, food security and preschool child diets in subsistence farming communities in Panama

Abstract Objectives: To explore impacts of a demonstration garden-based agricultural intervention on agricultural knowledge, practices and production, food security and preschool child diet diversity of subsistence farming households. Design: Observational study of households new to the intervention or participating for 1 or 5 years. Variables measured were agricultural techniques learned from the intervention and used, agricultural production, household food insecurity (FIS) and child diet diversity (DDS), over one agricultural cycle (during land preparation, growing and harvest months). Setting: Fifteen rural subsistence farming communities in Panama. Participants: Households participating in intervention (n 237) with minimum one preschool child. Results: After 1 year, participants had more learned and applied techniques, more staple crops produced and lower FIS and higher DDS during land preparation and growing months compared with those new to the intervention. After 5 years, participants grew more maize, chickens and types of crops and had higher DDS during growing months and, where demonstration gardens persisted, used more learned techniques and children ate more vitamin A-rich foods. Variables associated with DDS varied seasonally: during land preparation, higher DDS was associated with higher household durable asset-based wealth; during growing months, with greater diversity of vegetables planted and lower FIS; during harvest, with older caregivers, caregivers working less in agriculture, more diverse crops and receiving food from demonstration gardens. Conclusions: The intervention improved food production, food security and diets. Sustained demonstration gardens were important for continued use of new agricultural techniques and improved diets.
Comparative performance of advanced generations of maize hybrids with a local maize variety: agronomic and financial implications for smallholder farmers.

1 SUMMARY Experiments were conducted on farmers’ fields in a medium altitude ecosystem in Western Kenya to determine agronomic and financial implications of using farm-saved seed selected from advanced open pollinated generations of certified maize hybrids. First generation of certified seed (G1) of two commercial hybrids (H513 and H614), a local maize variety-Ababari and their respective advanced open pollinated generations two and three (G2 and G3) were evaluated with and without inorganic fertilizer. Significant differences (P<0.05) in maize grain yield were observed with the G1 consistently yielding more than their respective G2 and G3 generations. Fertilizer application resulted in significantly (P < 0.05) higher yield. The farmers’ variety revealed a marginal response to fertilizer but was significantly (P < 0.05) different at Ulumbi. Economic analyses showed that although certified seed with fertilizer had yield advantage of approximately one ton per hectare over unfertilized local maize, the marginal rate of return on hybrid seed and fertilizer was only 15%. This rate of return may not offer sufficient economic incentive for farmers to abandon the practice of growing local and recycled maize seeds without fertilizer given the associated costs of adopting a new technology. It is evident from this study that use of certified and inorganic fertilizer maize will significantly increase maize grain yield. However, the increase in grain yield cannot economically cushion the additional costs of certified seeds and fertilizer.
Online cognitive–behavioural therapy for traumatically bereaved people: study protocol for a randomised waitlist-controlled trial

Introduction The traumatic death of a loved one, such as death due to a traffic accident, can precipitate persistent complex bereavement disorder (PCBD) and comorbid post-traumatic stress disorder (PTSD) and depression. Waitlist-controlled trials have shown that grief-specific cognitive–behavioural therapy (CBT) is an effective treatment for such mental health problems. This is the first study that will examine the effectiveness of online CBT (vs waitlist controls) in a sample exclusively comprised of people bereaved by a traumatic death. Our primary hypothesis is that people allocated to the online CBT condition will show larger reductions in PCBD, PTSD and depression symptom levels at post-treatment than people allocated to a waitlist. We further expect that reductions in symptom levels during treatment are associated with reductions of negative cognitions and avoidance behaviours and the experience of fewer accident-related stressors. Moreover, the effect of the quality of the therapeutic alliance on treatment effects and drop-out rates will be explored. Methods and analysis A two-arm (online CBT vs waiting list) open-label parallel randomised controlled trial will be conducted. Participants will complete questionnaires at pretreatment and 12 and 20 weeks after study enrolment. Eligible for participation are Dutch adults who lost a loved one at least 1 year earlier due to a traffic accident and report clinically relevant levels of PCBD, PTSD and/or depression. Multilevel modelling will be used. Ethics and dissemination Ethics approval has been received by the Medical Ethics Review Board of the University Medical Center Groningen (METc UMCG: M20.252121). This study will provide new insights in the effectiveness of online CBT for traumatically bereaved people. If the treatment is demonstrated to be effective, it will be made publicly accessible. Findings will be disseminated among lay people (eg, through newsletters and media performances), our collaborators (eg, through presentations at support organisations), and clinicians and researchers (eg, through conference presentations and scientific journal articles). Trial registration number NL7497.
Associations of alcohol consumption and physical activity with lean type 2 diabetes mellitus among Korean adults: A prospective cohort study

Data on the association between alcohol consumption and the risk of type 2 diabetes mellitus (T2DM) have accumulated, but little has been reported about this association in terms of lean T2DM. The present study analyzed 10-year longitudinal data to investigate the association between alcohol consumption and T2DM risk among lean individuals. This prospective study included 2,366 male and female Koreans aged 40–69 years who were free of DM and had a body mass index (BMI) <23 kg/m2 during the baseline period between 2001 and 2012. Information on alcohol consumption, BMI, and incident cases of T2DM were identified by interviews and health examinations. To analyze the association between alcohol consumption and T2DM risk, Cox proportional hazard regression analysis was used. Alcohol drinkers consuming at least 16 g/day of alcohol (2 units/day) who maintained a BMI <23 kg/m2 over 10 years had a significantly higher T2DM risk even after controlling for BMI and potential risk factors. Compared with lifetime abstainers, multivariate hazard ratios (HR) [95% confidence interval] of T2DM were 1.74 [1.02, 2.95] for 16–30 g/day, 2.09 [1.16, 3.77] for 31–60 g/day, and 1.94 [1.07, 3.51] for >60g/day among alcohol drinkers. No protective effect of moderate alcohol consumption <16 g/day on T2DM risk was observed. Age, parental history of DM, and physical inactivity were also significant risk factors for lean T2DM. Alcohol consumption of at least 2 units/day increased T2DM risk among lean individuals. Abstaining from alcohol and physical activity may be beneficial for the prevention of lean T2DM.
Skin tattooing impairs sweating during passive whole-body heating.

Tattooing of the skin involves repeated needle insertions to deposit ink into the dermal layer of the skin, potentially damaging eccrine sweat glands and the cutaneous vasculature. This study tested the hypothesis that reflex increases in sweat rate (SR) and cutaneous vasodilation are blunted in tattooed skin (TAT) compared to adjacent healthy skin (CON) during a passive whole-body heat stress (WBH). Ten individuals (5M, 5F) with a sufficient area of tattooed skin participated in the study. Intestinal temperature (Tint), skin temperature (Tskin), skin blood flow (laser-Doppler flux; LDF), and SR were continuously measured during normothermic baseline (34 °C water perfusing a tube-lined suit) and WBH (increased Tint 1.0 °C via 48 °C water perfusing suit). SR throughout WBH was lower for TAT compared to CON (p=0.033). Accumulated sweating responses during WBH (area under curve) were attenuated in TAT relative to CON (23.1±12.9, 26.9±14.5 mg×cm-2, p=0.043). Sweating threshold, expressed as the onset of sweating in time or Tint from the initiation of WBH were not different between TAT and CON. Tattooing impeded the ability to obtain LDF measurements. These data suggest that tattooing functionally damages secretion mechanisms, affecting the reflex capacity of the gland to produce sweat, but does not appear to affect neural signaling to initiate sweating. Decreased sweating could impact heat dissipation especially when tattooing covers a higher percentage of body surface area and could be considered a potential long-term clinical side effect of tattooing.