Miracle-dz/UniFAR-FTUs huggingface dataset:
Two-Photon Vision in Age-Related Macular Degeneration: A Translational Study

The recently introduced term “two-photon vision” relates to the visual perception resulting from a simultaneous absorption of two photons by photoreceptors. In this study, we determined two-photon retinal sensitivity in age-related macular degeneration (AMD) and compared it that in normal aging. Microperimetry was performed with visible (white) light and infrared (IR) light, which was perceived as green in the two-photon stimulation. In total, 45 subjects were included with one (better) eye studied. Furthermore, best-corrected visual acuity (VA) and ocular straylight were assessed. AMD resulted in decreased median (interquartile range) logMAR VA, i.e., 0.15 (0.05; 0.24), which in normal eyes was −0.02 (−0.06; 0.02). The two groups showed comparable straylight levels. Sensitivity to IR light was significantly lower in the AMD group (p < 0.001): 8.3 (7.4, 9.3) dB than in controls 10.7 (9.7, 11.2) dB. AMD also significantly affected visible light sensitivity (p < 0.001): 14.0 (11.0; 15.5) dB vs. 18.0 (16.3; 18.9) dB. Notably, the two-photon approach yielded a lower data spread. In conclusion, AMD considerably impairs retinal sensitivity measured in the single- and two-photon realm. However, two-photon-vision microperimetry may improve the testing accuracy and offer an additional diagnostic parameter (beyond VA measurements) for retinal function assessment.
The Study on the Relationship between Normalized Difference Vegetation Index and Fractional Green Canopy Cover in Five Selected Crops

Crop models are of great use and importance in modern agriculture. Most models imply spatial vegetation indices, such as NDVI, or canopy cover characteristics, such as FGCC, to provide estimation of crops conditions and forecast productivity. The purpose of the study was to (1) determine the possibility of mutual conversion between spatial NDVI and Canopeo-derived FGCC in five crops (grain corn, sunflower, tomato, millet, and winter wheat) and (2) estimate the precision of such a conversion. The data set of the study was formed by the OneSoil AI derived satellite imagery on NDVI for the studied crops in different stages of their growing season combined with Canopeo-processed photographs of vegetating crops in the field with FGCC percentage calculation. The sets of NDVI and FGCC values were paired up and then statistically processed to obtain polynomial equations of NDVI into FGCC and inverse conversion for each crop. The results of the study revealed that mutual conversion between spatial NDVI and Canopeo-derived FGCC is possible. There is a strong direct correlation (R2 within 0.6779–0.9000 depending on the crop) between the studied indices for all crops. Close-growing crops, especially winter wheat, showed the highest correlation, while row crops and especially tomatoes had a less strong relationship between vegetation indices. The models for mutual conversion between FGCC and NDVI could be incorporated into the yield simulation models to improve the forecasting capacities.
Distinguishing Evolutionary Conservation from Derivedness

While the concept of “evolutionary conservation” has enabled biologists to explain many ancestral features and traits, it has also frequently been misused to evaluate the degree of changes from a common ancestor, or “derivedness”. We propose that the distinction of these two concepts allows us to properly understand phenotypic and organismal evolution. From a methodological aspect, “conservation” mainly considers genes or traits which species have in common, while “derivedness” additionally covers those that are not commonly shared, such as novel or lost traits and genes to evaluate changes from the time of divergence from a common ancestor. Due to these differences, while conservation-oriented methods are effective in identifying ancestral features, they may be prone to underestimating the overall changes accumulated during the evolution of certain lineages. Herein, we describe our recently developed method, “transcriptomic derivedness index”, for estimating the phenotypic derivedness of embryos with a molecular approach using the whole-embryonic transcriptome as a phenotype. Although echinoderms are often considered as highly derived species, our analyses with this method showed that their embryos, at least at the transcriptomic level, may not be much more derived than those of chordates. We anticipate that the future development of derivedness-oriented methods could provide quantitative indicators for finding highly/lowly evolvable traits.
The Identification of Common Models Applied for the Integration of Management Systems: A Review

The paper presents a synthesis of strategies, benefits, and barriers in the integration management systems (IMS) implementation at the organizational level. The research is based on an assessment of 103 papers, 7 books, 12 standards, and 7 sites. The analysis of works aims to highlight the common integration models of several IMS, aspects related to IMS implementation, benefits of IMS implementations, and the nature of integration strategies. The research results present valuable information which can be used by top managers of organizations, certification bodies, and consulting organizations to encourage the implementation of integrated management systems.
The Human Induced Pluripotent Stem Cell Test as an Alternative Method for Embryotoxicity Testing

The evaluation of substances for their potency to induce embryotoxicity is controlled by safety regulations. Test guidelines for reproductive and developmental toxicity rely mainly on animal studies, which make up the majority of animal usage in regulatory toxicology. Therefore, there is an urgent need for alternative in vitro methods to follow the 3R principles. To improve human safety, cell models based on human cells are of great interest to overcome species differences. Here, human induced pluripotent stem cells (hiPSCs) are an ideal cell source as they largely recapitulate embryonic stem cells without bearing ethical concerns and they are able to differentiate into most cell types of the human body. Here, we set up and characterized a fetal bovine serum (FBS)-free hiPSC-based in vitro test method, called the human induced pluripotent stem cell test (hiPS Test), to evaluate the embryotoxic potential of substances. After 10 days in culture, hiPSCs develop into beating cardiomyocytes. As terminal endpoint evaluations, cell viability, qPCR analyses as well as beating frequency and area of beating cardiomyocytes by video analyses are measured. The embryotoxic positive and non-embryotoxic negative controls, 5-Fluorouracil (5-FU) and Penicillin G (PenG), respectively, were correctly assessed in the hiPS Test. More compounds need to be screened in the future for defining the assay’s applicability domain, which will inform us of the suitability of the hiPS Test for detecting adverse effects of substances on embryonic development.
Fabrication of TiO2/NiO p-n Nanocomposite for Enhancement Dye Photodegradation under Solar Radiation

A p-n nanocomposite based on TiO2 nanotubes (NTs) and NiO nanoparticles (NPs) was designed and optimized in this study to improve the photocatalytic performance of methylene blue (MB). The hydrothermal technique has been used to produce TiO2/NiO nanocomposites with different NiO NPs weight ratios; 1TiO2:1NiO, 1TiO2:2NiO, and 1TiO2:3NiO. The crystal phase, chemical composition, optical properties, and morphology of TiO2/NiO were explored by various techniques. TiO2 NTs have a monoclinic structure, while NiO NPs have a cubic structure, according to the structural study. The bandgap of TiO2 NTs was reduced from 3.54 eV to 2.69 eV after controlling the NiO NPs weight ratio. The TiO2/2NiO nanocomposite showed the best photodegradation efficiency. Within 45 min of solar light irradiation, the efficiency of MB dye degradation using TiO2/2NiO hits 99.5% versus 73% using pure TiO2 NTs. Furthermore, the catalytic photodegradation efficiency did not deteriorate significantly even after five reusability cycles, intimating the high stability of the TiO2/2NiO nanocomposite. This suggests that the loading of NiO NPs into TiO2 NTs lowers the recombination of photo-produced electron/hole pairs and enlarged solar spectral response range, which results in improved photocatalytic activity. The mechanism of charge transfer in the TiO2/NiO and kinetic models were discussed for the photodegradation of MB.
Impacts of Wildflower Interventions on Beneficial Insects in Fruit Crops: A Review

Simple Summary Declines in insects, including pollinators, are leading to public pressure for governments and landowners to intervene. Although integrated pest management, used to preserve the natural enemies of pests, has been in place for decades, promoting the preservation of pollinators is more recent, especially on farmed land. To enable fruit production, which is less environmentally damaging, natural enemies and pollinators are key. In addition, a reduction in available pesticides is forcing fruit growers to reconsider their management practices. One intervention that is increasingly being used is the provision of floral resources as wildflower areas or cover crops to encourage natural enemies and pollinators. This review brings together the literature on the benefits and costs of provisioning flora in the vicinity of fruit crops. It highlights that most impacts of floral resource provision in the vicinity of fruit crops are beneficial or benign. Abstract Integrated pest management (IPM) has been practiced by the fruit industry for at least 30 years. Naturally occurring beneficial insects have been encouraged to thrive alongside introduced predatory insects. However, Conservation Biological Control (CBC) and augmented biocontrol through the release of large numbers of natural enemies is normally only widely adopted when a pest has become resistant to available conventional pesticides and control has begun to break down. In addition, the incorporation of wild pollinator management, essential to fruit production, has, in the past, not been a priority but is now increasingly recognized through integrated pest and pollinator management (IPPM). This review focuses on the impacts on pest regulation and pollination services in fruit crops through the delivery of natural enemies and pollinating insects by provisioning areas of fruiting crops with floral resources. Most of the studies in this review highlighted beneficial or benign impacts of floral resource prevision to fruit crops. However, placement in the landscape and spill-over of beneficial arthropods into the crop can be influential and limiting. This review also highlights the need for longer-term ecological studies to understand the impacts of changing arthropod communities over time and the opportunity to tailor wildflower mixes to specific crops for increased pest control and pollination benefits, ultimately impacting fruit growers bottom-line with less reliance on pesticides.
Quantitatively Unraveling Hierarchy of Factors Impacting Virgin Olive Oil Phenolic Profile and Oxidative Stability

A single phenolic group and even a compound play different roles in the sensory properties and stability of virgin olive oil (VOO), which in turn are strongly influenced by several factors. Understanding the causes of differences in phenolic compound composition and oxidative stability (OS) in VOOs is essential for targeted and timely harvest and processing while maintaining desired oil quality. The phenolic profile and OS of two monocultivar VOOs (Oblica and Leccino) grown in two geographical sites of different altitudes (coastal plain and hilly hinterland) were analyzed throughout the ripening period over two years. Concentration of secoiridoids was 30% higher in the Oblica than in the Leccino VOOs, which in turn had significantly higher values of OS. Both cultivars had more than twice as high concentrations of the two most abundant phenolic compounds, the dialdehyde form of decarboxymethyl oleuropein aglycone and the dialdehyde form of decarboxymethyl ligstroside aglycone, and OS values in a colder growing site of higher altitude. Among the studied monocultivar VOOs, the secoiridoid group did not behave equally during ripening. The hierarchy of different influencing factors was investigated using multivariate statistics and revealed: cultivar > geographical site > harvest period > growing season. In addition, the possibility of traceability of VOO using molecular markers was investigated by establishing SSR profiles of oils of the studied cultivars and comparing them with SSR profiles of leaves.
Detecting Micro- and Nanoplastics Released from Food Packaging: Challenges and Analytical Strategies

Micro- and nanoplastic (pMP and pNP, respectively) release is an emerging issue since these particles constitute a ubiquitous and growing pollutant, which not only threatens the environment but may have potential consequences for human health. In particular, there is concern about the release of secondary pMP and pNP from the degradation of plastic consumer products. The phenomenon is well-documented in relation to plastic waste in the environment but, more recently, reports of pMP generated even during the normal use of plastic food contact materials, such as water bottles, tea bags, and containers, have been published. So far, a validated and harmonized strategy to tackle the issue is not available. In this study, we demonstrate that plastic breakdown to pMP and pNP can occur during the normal use of polyethylene (PE) rice cooking bags and ice-cube bags as well as of nylon teabags. A multi-instrumental approach based on Raman microscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and particular attention on the importance of sample preparation were applied to evaluate the chemical nature of the released material and their morphology. In addition, a simple method based on Fourier transform infrared (FT-IR) spectroscopy is proposed for pNP mass quantification, resulting in the release of 1.13 ± 0.07 mg of nylon 6 from each teabag. However, temperature was shown to have a strong impact on the morphology and aggregation status of the released materials, posing to scientists and legislators a challenging question: are they micro- or nanoplastics or something else altogether?
Early-Stage Lung Cancer Patients’ Perceptions of Presurgical Discussions

Background Patients with early-stage non–small-cell lung cancer (NSCLC) have high survival rates, but patients often say they did not anticipate the effect of the surgery on their postsurgical quality of life (QoL). This study adds to the literature regarding patient and surgeon interactions and highlights the areas where the current approach is not providing good communication. Design Since its start in 2016, the Initiative for Early Lung Cancer Research on Treatment (IELCART), a prospective cohort study, has enrolled 543 patients who underwent surgery for stage I NSCLC within the Mount Sinai Health System. Presurgical patient and surgeon surveys were available for 314 patients, postsurgical surveys for 420, and both pre- and postsurgical surveys for 285. Results Of patients with presurgical surveys, 31.2% said that their surgeon recommended multiple types of treatment. Of patients with postsurgical surveys, 85.0% felt very well prepared and 11.4% moderately well prepared for their postsurgical recovery. The median Functional Assessment of Cancer Therapy–Lung Cancer score and social support score of the patients who felt very well prepared was significantly higher than those moderately or not well prepared (24.0 v. 22.0, P < 0.001) and (5.0 [interquartile range: 4.7–5.0] v. 5.0 [IQR: 4.2–5.0], p = 0.015). Conclusions This study provides insight into the areas where surgeons are communicating well with their patients as well as the areas where patients still feel uninformed. Most surgeons feel that they prepare their patients well or very well for surgical recovery, whereas some patients still feel that their surgeons did not prepare them well for postsurgical recovery. Surgeons may want to spend additional time emphasizing postsurgical recovery and QoL with their patients or provide their patients with additional avenues to get their questions and concerns addressed. Highlights Pretreatment discussions could help surgeons understand patient priorities and patients understand the anticipated outcomes for their surgeries. There is an association between feeling prepared for surgery and higher quality of life and social support scores after adjustment for confounders. Despite these pretreatment discussions, patients still feel that they are not well prepared about what to expect during their postsurgical recovery.
The Effect of Service Quality on Trust and Its Impact on Willingness to Pay Tax at Finance and Asset Management of Banda Aceh City

This study aims to determine the effect of the tax quality service on trust as well as its impact on the willingness to pay taxes of restaurant (a case study at the Department of Finance and Asset Management of Banda Aceh City). The current study is to explain how important of trust as a variable to play significant role in this research model. Population of this study is the overall restaurant in the city of Banda Aceh. The number of samples of this study is 250 respondents. The analytical method used is structural equation modeling (SEM) by using AMOS software. Results indicate that quality of service has a positive and significant effect on trust and its impact on the willingness to pay tax. Quality of service has indirectly effected on the willingness to pay tax through trust, where the factor of trust acts as a partially mediator. Implications of this research is the Department of Finance and Asset Management of Banda Aceh City needs to build trust through improving quality of service in order to impact on increasing the willingness to pay taxes of restaurant.
Genomics of Plasma Cell Leukemia

Simple Summary Plasma cell leukemia (PCL) is a very aggressive plasma cell disorder with a dismal prognosis, despite the therapeutic progress made in the last few years. The implementation of genomic high-throughput technologies in the clinical setting has revealed new insights into the genomic landscape of PCL, some of which may have an impact on the development of novel therapeutic approaches. The purpose of this review is to provide a comprehensive overview and update of the genomic studies carried out in PCL. Abstract Plasma cell leukemia (PCL) is a rare and highly aggressive plasma cell dyscrasia characterized by the presence of clonal circulating plasma cells in peripheral blood. PCL accounts for approximately 2–4% of all multiple myeloma (MM) cases. PCL can be classified in primary PCL (pPCL) when it appears de novo and in secondary PCL (sPCL) when it arises from a pre-existing relapsed/refractory MM. Despite the improvement in treatment modalities, the prognosis remains very poor. There is growing evidence that pPCL is a different clinicopathological entity as compared to MM, although the mechanisms underlying its pathogenesis are not fully elucidated. The development of new high-throughput technologies, such as microarrays and new generation sequencing (NGS), has contributed to a better understanding of the peculiar biological and clinical features of this disease. Relevant information is now available on cytogenetic alterations, genetic variants, transcriptome, methylation patterns, and non-coding RNA profiles. Additionally, attempts have been made to integrate genomic alterations with gene expression data. However, given the low frequency of PCL, most of the genetic information comes from retrospective studies with a small number of patients, sometimes leading to inconsistent results.
Perceived Environmental Attributes: Their Impact on Older Adults’ Mental Health in Malaysia

In Malaysia, the population of older adults will increase in the coming years. In this context, there is a requirement to build an age-friendly environment to enable the elderly to age healthily. Many studies have shown that a built environment that allows older adults to age in place improves their mental health. However, person-environment analysis that considers mental well-being has remained rare for older adults living in Malaysia. This study examines the relationship between Malaysian seniors’ perceptions of their surroundings at home and in the neighborhood and their mental health. Using stratified sampling, 510 seniors aged 60 and over were interviewed. The results showed that accessibility (p-value 0.033, 95% CI for coefficients 0.006, 0.146), environmental qualities (0.015, 0.014, 0.129) and neighborhood problems (0.000, −0.299, −0.146) were significant determinants of elderly people’s mental health. With respect to respondents’ socio-demographic characteristics, female elderly (0.000, 0.616, 0.782), older adults with an elementary education (0.000, 0.263, 0.685) or a college degree (0.026, 0.019, 0.294), being married (0.005, 0.047, 0.259), the ability to drive (0.000, 0.993, 1.315), the number of dependents in the family (0.003, −0.060, −0.012), and homeownership (0.000, −0.602, −0.271) were significantly related to mental well-being.
Esthetic Assessment following Ridge Augmentation, Late Implant Placement and Immediate Esthetic Reconstruction of the Atrophic Anterior Maxilla

Purpose: Evaluate the esthetic outcome of ridge augmentation using cancellous bone-block allografts, late implant placement, and immediate loading in the atrophic anterior maxilla, by PES (pink esthetic score) and WES (white esthetic score) indexes. Materials and Methods: Retrospective cohort study. Inclusion criteria were bone loss of at least 3 mm horizontally and 3 mm vertically according to preliminary CBCT; ridge augmentation using cancellous bone-block allografts; six months later the implant insertion and immediately loaded. PES-WES index was used for esthetic assessment of soft tissues surrounding the final implant-supported prosthesis (ISP). Results: All twenty-five successive individuals were included. The mean follow-up was 12.1 ± 56 months (range, 42–90 months). The mean PES index and WES index were 7 ± 1.74 (range: 5–10) and 8.4 ± 2.12 (range: 5–10), respectively. The mean total combination of PES index and WES index (PES/WES) was 15.3 ± 2.85 (range: 12–20). All ISPs had an overall score >12 (the defined threshold of clinical acceptability). Conclusions: Ridge augmentation in the atrophic anterior maxilla using cancellous bone-block allografts and immediate loading allows a stable esthetic result of the soft and hard tissues over the years (follow-up of 42–90 months).
MicroRNA-Based and Proteomics Fingerprinting of Avena sativa L. Genotypes

: Regulatory potential of microRNAs in growth, metabolism, and stress adaptation is well known. In total, 15 common oat genotypes were performed by miRNA-based markers. Markers involved in MiRNAs sequences provide an effective type of putative functional markers. Markers involved to photosynthesis regulation, growth, and biomass production (miR408, miR156) generated 40% of all ampliﬁed loci. Those associated with nutrition accumulation and homeostasis (miR827 and miR399) generated 29% and stress-responsive markers (miR398 and miR858) 31%. Proteomics approaches (SDS and A-PAGE electrophoresis) detected sufﬁcient diversity between the analyzed samples and genetic-related dendrograms were constructed based on the electrophoretic proﬁles.