Miracle-dz/UniFAR-FTUs huggingface dataset:
Real‐world utility of early measurable residual disease assessments by multi‐parametric flow cytometry in adult patients with B‐lymphoblastic leukemia receiving Hyper‐CVAD induction chemotherapy

Multi‐parametric flow cytometry (MFC) has a well‐established role in measurable residual disease (MRD) monitoring in patients with B‐lymphoblastic leukemia (B‐ALL). However, the optimal time‐point (TP) for early MRD testing and associated prognostic impact remain undefined in adult B‐ALL patients receiving Hyper‐CVAD induction chemotherapy. To evaluate the utility of MRD analysis after one cycle (TP1) in comparison to MRD analysis after two cycles (TP2) of induction treatment with Hyper‐CVAD chemotherapy, we studied 49 adult B‐ALL patients over a 10‐year period (2010–2020) who had available bone marrow samples for morphological and MFC MRD assessments at the two separate TPs. Median times to TP1 and TP2 relative to start of treatment were 21 and 45 days, respectively. When censored at transplant, achievement of MRD negativity at TP1 was not associated with a statistically significant improvement in either event‐free survival (EFS) (p = .426) or overall survival (OS) (p = .335) when compared to patients with MRD positivity. In contrast, achieving MRD negativity at TP2 was associated with a statistically significant improvement in both EFS (p = ·005) and OS (p = .047) over patients who remained MRD positive. Multivariate analysis demonstrated that KMT2A‐rearrangement and MRD positivity at TP2 were the only significant predictors of outcome, correlating with worse EFS and OS. Therefore, in the absence of residual morphologic disease, MRD analysis after one cycle of Hyper‐CVAD induction chemotherapy did not provide additional benefit with regard to risk stratification or correlation with survival outcomes when compared to MRD testing after two cycles of Hyper‐CVAD in adult B‐ALL patients.
Youth and Young Adult-targeted E-cigarette Warnings and Advertising Messages: An Experiment with Young Adults in the US

ABSTRACT Warnings specifically focused on harm to younger users have been understudied in vaping warning research, even while vaping products may appeal specifically to a younger population through implicit advertising strategies. This study examined how youth and young adult-focused e-cigarette health warning messages and implicit advertising strategies influence affective responses, risk perceptions, cognitive elaboration about e-cigarette harms, and willingness to vape in the future. We recruited young adults (who, at the time, were not smoking combustible cigarettes) aged 18–25 to participate in an online survey experiment with a 3 (warning label type: current FDA/youth and young adult risk-focused/none) × 3 (advertising health message strategy: explicit/implicit/none) + 3 (non-vaping products control) design. The results show a main effect for warning such that both FDA and targeted warnings increased negative affect and decreased positive affect compared to no warning. Moreover, the youth and young adult-focused warning boosted youth-specific harm beliefs and cognitive elaboration relative to control and the FDA warning, which did not differ from one another. Implicit health messages produced greater positive affect relative to explicit messages and no message, but the ad strategy manipulations did not influence other outcomes. While the population studied here with a single exposure reported no effects of either manipulation on willingness to vape, previous research has associated similar emotions and cognitions with lowered intentions to vape. Regulatory bodies should consider further exploration of vaping warnings that emphasize youth and young adult-specific harms to educate young people about relevant risks.
How central and peripheral vision influence focal and ambient processing during scene viewing

Central and peripheral vision carry out different functions during scene processing. The ambient mode of visual processing is more likely to involve peripheral visual processes, whereas the focal mode of visual processing is more likely to involve central visual processes. Although the ambient mode is responsible for navigating space and comprehending scene layout, the focal mode gathers detailed information as central vision is oriented to salient areas of the visual field. Previous work suggests that during the time course of scene viewing, there is a transition from ambient processing during the first few seconds to focal processing during later time intervals, characterized by longer fixations and shorter saccades. In this study, we identify the influence of central and peripheral vision on changes in eye movements and the transition from ambient to focal processing during the time course of scene processing. Using a gaze-contingent protocol, we restricted the visual field to central or peripheral vision while participants freely viewed scenes for 20 seconds. Results indicated that fixation durations are shorter when vision is restricted to central vision compared to normal vision. During late visual processing, fixations in peripheral vision were longer than those in central vision. We show that a transition from more ambient to more focal processing during scene viewing will occur even when vision is restricted to only central vision or peripheral vision.
Immunological evidence of an early seroconversion to oncogenic Merkel cell polyomavirus in healthy children and young adults

Oncogenic Merkel cell polyomavirus (MCPyV) provokes a widespread and asymptomatic infection in humans. Herein, sera from healthy children and young adults (HC, n = 344) aged 0–20 years old were evaluated for anti‐MCPyV immunoglobulin G (IgG) and IgM antibodies employing a recently developed immunoassay. Serum MCPyV IgG data from healthy subjects (HS, n = 510) and elderlies (ES, n = 226), aged 21–65/66–100 years old, from our previous studies, were included. The anti‐MCPyV IgG and IgM rates in HC sera were 40.7% and 29.7%, respectively. A lower prevalence of anti‐MCPyV IgGs was found in HC aged 0–5 years old (13%) compared to 6–10 (52.3%), 11–15 (60.5%) and 16–20 years old (61.6%) cohorts. Age‐stratified HCs exhibited similar anti‐MCPyV IgM rates (27.9%–32.9%). Serological profiles indicated that anti‐MCPyV IgGs and IgMs had low optical densities (ODs) during the first years of life, while IgM ODs appeared to decrease throughout young adulthood. A lower anti‐MCPyV IgGs rate was found in HC (40.7%) than HS (61.8%) and ES (63.7%). Upon the 5‐years range age‐stratification, a lower anti‐MCPyV IgGs rate was found in the younger HC cohort aged 0–5 years old compared to the remaining older HC/HS/ES cohorts (52.3%–72%). The younger HC cohort exhibited the lowest anti‐MCPyV IgG ODs than the older cohorts. Low anti‐MCPyV IgMs rates and ODs were found in the 21–25 (17.5%) and 26–30 (7.7%) years old cohorts. Our data indicate that, upon an early‐in‐life seroconversion, the seropositivity for oncogenic MCPyV peaks in late childhood/young adulthood and remains at high prevalence and relatively stable throughout life.
Redshift of photons penetrating a hot plasma

A new interaction, plasma redshift, is derived, which is important only when photons penetrate a hot, sparse electron plasma. The derivation of plasma redshift is based entirely on conventional axioms of physics. When photons penetrate a cold and dense plasma, they lose energy through ionization and excitation, Compton scattering on the individual electrons, and Raman scattering on the plasma frequency. But in sparse hot plasma, such as in the solar corona, the photons lose energy also in plasma redshift. The energy loss per electron in the plasma redshift is about equal to the product of the photon's energy and one half of the Compton cross-section per electron. In quiescent solar corona, this heating starts in the transition zone to the corona and is a major fraction of the coronal heating. Plasma redshift contributes also to the heating of the interstellar plasma, the galactic corona, and the intergalactic plasma. Plasma redshift explains the solar redshifts, the redshifts of the galactic corona, the cosmological redshifts, the cosmic microwave background, and the X-ray background. The plasma redshift explains the observed magnitude-redshift relation for supernovae SNe Ia without the big bang, dark matter, or dark energy. There is no cosmic time dilation. The universe is not expanding. The plasma redshift, when compared with experiments, shows that the photons' classical gravitational redshifts are reversed as the photons move from the Sun to the Earth. This is a quantum mechanical effect. As seen from the Earth, a repulsion force acts on the photons. This means that there is no need for Einstein's Lambda term. The universe is quasi-static, infinite, and everlasting.
Posterior Horn Lateral Meniscal Oblique Radial Tear in Acute Anterior Cruciate Ligament Reconstruction Incidence and Outcomes After All-Inside Repair: Clinical and Second-Look Arthroscopic Evaluation

Background: The term posterior horn lateral meniscal oblique radial tear (LMORT) has emerged to characterize the tear patterns of the lateral meniscus in many patients with acute anterior cruciate ligament (ACL) injury. There is a lack of data regarding the exact incidence according to the types of LMORT and clinical outcomes. Purposes: (1) To investigate the incidence of LMORT according to type in patients with acute ACL reconstruction (ACLR) and (2) to identify healing status after repair of LMORT via second-look arthroscopy and clinical outcomes. Study Design: Case series; Level of evidence: 4. Methods: Patients who underwent primary ACLR within 6 months of injury were retrospectively reviewed. The LMORT was classified into 4 types based on the severity and distance from the root: type 1 (partial tear <10 mm from the root), type 2 (complete tear <10 mm from the root), type 3 (partial tear >10 mm from the root), type 4a (complete tear >10 mm from the root), and type 4b (type 4a with longitudinal tear at the meniscocapsular junction). Only patients with LMORT were isolated, and the clinical outcomes were compared according to the healing status of LMORT in second-look arthroscopy. Results: Of 635 patients with ACLR, LMORT was identified in 97 patients (15.3%), and type 4 LMORT accounted for the largest proportion (n = 62; 32.6%) of 190 lateral meniscal tears. In 79 patients with LMORT who satisfied the 2-year follow-up period, all patient-reported outcomes (PROs), including the Lysholm (preoperative, 64.1; postoperative, 88.2) and International Knee Documentation Committee subjective (preoperative, 50.5; postoperative, 82.9) scores, were significantly improved (P < .001) 31.8 months postoperatively. Of the 61 patients who underwent second-look arthroscopy, 49 (80.3%) were classified into the complete healing group. There was no significant difference in postoperative PROs between the complete and partial healing groups. Conclusion: The incidence of LMORT was 15.3% in patients with acute ACL injury, and type 4 LMORT was the most common type. Complete healing of LMORT was achieved in 80.3% of patients who underwent second-look arthroscopy, and the PROs were significantly improved postoperatively. Good clinical results can be achieved if the LMORT is repaired as much as possible during ACLR.
Quantum and Classical Phases of the Pyrochlore Heisenberg Model with Competing Interactions

We investigate the quantum Heisenberg model on the pyrochlore lattice for a generic spin-$S$ in the presence of nearest-neighbor $J_{1}$ and second-nearest-neighbor $J_{2}$ exchange interactions. By employing the pseudofermion functional renormalization group (PFFRG) method, we find, for $S=1/2$ and $S=1$, an extended quantum spin liquid phase centered around $J_{2}=0$, which is shown to be robust against the introduction of breathing anisotropy. The effects of temperature, quantum fluctuations, breathing anisotropies, and a $J_{2}$ coupling on the nature of the scattering profile, in particular, the pinch points are studied. For the magnetic phases of the $J_{1}$-$J_{2}$ model, quantum fluctuations are shown to strongly renormalize phase boundaries compared to the classical model and shift the ordering wave vectors of spiral magnetic states, however, no new magnetic orders are found to be stabilized.
Prognostic impact of unsupervised early assessment of bulk and leukemic stem cell measurable residual disease in acute myeloid leukemia.

PURPOSE Acute myeloid leukemias (AML) are clonal diseases that develop from leukemic stem cells (LSCs) that carry an independent prognostic impact on the initial response to induction chemotherapy, demonstrating the clinical relevance of LSC abundance in AML. In 2018, the European LeukemiaNet has published recommendations for the detection of measurable residual disease (Bulk MRD) and suggested the exploration of LSC MRD and the use of multiparametric displays. EXPERIMENTAL DESIGN We evaluated the performance of unsupervised clustering for the post-induction assessment of bulk and LSC MRD in 155 AML patients who received intensive conventional chemotherapy treatment. RESULTS The median overall survival (OS) for Bulk+ MRD patients was 16.7 months and was not reached for negative patients (Hazard Ratio, HR:3.82, p<0.0001). The median OS of LSC+ MRD patients was 25.0 months and not reached for negative patients (HR:2.84, p=0.001). Interestingly, one-year (y) and 3-y OS were 60% and 39% in Bulk+, 91% and 52% in Bulk-LSC+ and 92% and 88% in Bulk-LSC-. CONCLUSION In this study, we confirm the prognostic impact of post-induction MFC Bulk MRD in AML patients. Focusing on LSCs, we identified a group of patients with negative Bulk MRD but positive LSC MRD (25.8% of our cohort) with an intermediate prognosis, demonstrating the interest of MRD analysis focusing on leukemic chemoresistant subpopulations.
Tonic extracellular glutamate and ischaemia: glutamate antiporter system xc − regulates anoxic depolarization in hippocampus

In stroke, the sudden deprivation of oxygen to neurons triggers a profuse release of glutamate that induces anoxic depolarization (AD) and leads to rapid cell death. Importantly, the latency of the glutamate‐driven AD event largely dictates subsequent tissue damage. Although the contribution of synaptic glutamate during ischaemia is well‐studied, the role of tonic (ambient) glutamate has received far less scrutiny. The majority of tonic, non‐synaptic glutamate in the brain is governed by the cystine/glutamate antiporter, system xc−. Employing hippocampal slice electrophysiology, we showed that transgenic mice lacking a functional system xc− display longer latencies to AD and altered depolarizing waves compared to wild‐type mice after total oxygen deprivation. Experiments which pharmacologically inhibited system xc−, as well as those manipulating tonic glutamate levels and those antagonizing glutamate receptors, revealed that the antiporter's putative effect on ambient glutamate precipitates the ischaemic cascade. As such, the current study yields novel insight into the pathogenesis of acute stroke and may direct future therapeutic interventions.
Incidence of Poststroke Epilepsy: An Executive Summary

Purpose There is a plethora of literature describing stroke risk factors, cause, incidence, treatment, transition, and secondary prevention. 1 – 4 Poststroke literature often focuses on a variety of outcomes such as mortality, readmission, functional status, depression, return to work, secondary stroke, and caregiver or care-partner outcomes. 5 – 7 How-ever, relatively little attention has been given to the incidence of poststroke new-onset seizure and poststroke new-onset epilepsy. This executive summary aims to provide a succinct overview of the recent (last decade) evidence on incidence and presentation of new-onset seizures that occur in stroke survivors. late associated seizure recurrence rate > 9
Influence of cardioplegic solution on incidence of delirium after CABG surgery: Use of Calafiore blood cardioplegia versus HTK ‐ Bretschneider ‐ solution in a single‐center retrospective analysis from 2017 to 2021

Purpose of the present study is an evaluation of postoperative incidence for delirium after coronary artery bypass surgery (CABG). Study addressed whether application of Histidine‐Tryptophan‐Ketoglutarate (HTK) solution (Bretschneider) or blood cardioplegia (Calafiore) is associated with increased of postoperative delirium cases.
Predicting Effectiveness of Antihypertensive Medications for Heart Failure based on Longitudinal Patient Records and Deep Learning

Drug treatment for heart failure (HF) condition includes different medications. As patients could respond variably to a particular medication, being able to predict drug effectiveness is crucial for personalized treatment. Laboratory tests in EHR summarize different aspects of the patient's physiological process related to a diagnosis, where blood pressure (BP) is deemed a critical hemodynamic parameter for HF prognosis. This work first proposes a novel method based on combinations of different clinical end points to generate the positive and negative samples corresponding to HF patients on whom the drug is effective and not effective respectively. We then formulate drug effectiveness prediction as a time series classification problem and experiment with several deep learning models, leveraging the temporal BP laboratory measurements from EHR as the features. Over thorough comparative evaluations among 3 categories of HF medications and two types of lab features, we achieved the best F1 performance of ~0.97.
Patient Satisfaction and Oral Health-related Quality of Life (ohrqol) of Edentulous People after Mandibular Denture Relining With a Soft-liner Material: A Pilot Study

Oral health-related quality of life indicators have been effectively used to assess the influence of a range of dental treatments and interventions on patient satisfaction and acceptance. The purpose of this study was to evaluate the impact of the relining of mandibular dentures with a soft-liner material, using the Oral Health Assessment Index (OHIP-14). Forty-two full denture wearers were selected from patients who attended 9 private prosthetic clinics in New South Wales, Australia. All participants had their lower dentures relined with a soft-liner material, according to the manufacturer’s instructions. Oral health-related quality of life was evaluated before and 1 month after relining using the Oral Health Assessment Index questionnaire. A simple descriptive quantitative analysis of the survey results was undertaken, including a comparison of median scores using the Wilcoxon paired test and binomial probability test. In all the outcome variables measured, there was a noticeable positive effect from the denture relining. Specifically, mandibular denture relining with a silicon-based soft-liner material has a positive impact on patient satisfaction and oral health-related quality of life (OHRQoL) of edentulous patients. Patient satisfaction and oral health-related quality of life (OHRQoL) of edentulous patients can be significantly improved by the use of soft-liner materials.
Enhanced Rishirilide Biosynthesis by a Rare In-Cluster Phosphopantetheinyl Transferase in Streptomyces xanthophaeus

Genome mining has been a vital means for natural product drug discovery in the postgenomic era. The rishirilide-type polyketides have attracted attention due to their potent bioactivity, but the poor robustness of production hosts has limited further research and development. ABSTRACT Phosphopantetheinyl transferases (PPTases) play important roles in activating apo-acyl carrier proteins (apo-ACPs) and apo-peptidyl carrier proteins (apo-PCPs) in both primary and secondary metabolism. PPTases catalyze the posttranslational modifications of those carrier proteins by covalent attachment of the 4′-phosphopantetheine group to a conserved serine residue. The protein-protein interactions between a PPTase and a cognate acyl or peptidyl carrier protein have important regulatory functions in microbial biosynthesis, but the molecular mechanism underlying their specific recognition remains elusive. In this study, we identified a new rishirilide biosynthetic gene cluster with a rare in-cluster PPTase from Streptomyces xanthophaeus no2. The function of this Sfp-type PPTase, SxrX, in rishirilide production was confirmed using genetic mutagenesis and biochemical characterization. We applied molecular modeling and site-directed mutagenesis to identify key residues mediating the protein-protein interaction between SxrX and its cognate ACP. In addition, six natural products were isolated from wild-type S. xanthophaeus no2 and the ΔsxrX mutant, including rishirilide A and lupinacidin A, that exhibited antimicrobial and anticancer activities, respectively. SxrX is the first Sfp-type PPTase identified from an aromatic polyketide biosynthetic gene cluster and shown to be responsible for high-level production of rishirilide derivatives. IMPORTANCE Genome mining has been a vital means for natural product drug discovery in the postgenomic era. The rishirilide-type polyketides have attracted attention due to their potent bioactivity, but the poor robustness of production hosts has limited further research and development. This study not only identifies a hyperproducer of rishirilides but also reveals a rare, in-cluster PPTase SxrX that plays an important role in boosting rishirilide biosynthesis. Experimental and computational investigations revealed new insights on the protein-protein interaction between SxrX and its cognate ACP with wide implications for understanding polyketide biosynthesis.
Solar neutrino physics on the beginning of 2017

This writeup is a review of current hot topics on solar neutrinos. It is based on a talk at the conference "Neutrinos: the quest for a new physics scale", held at the CERN on March 2017, where the Organizers entrusted me with a discussion of the provocative question "whether solar neutrino physics is over". Rather than providing a straight (negative) answer, in view of an audience consisting mostly of colleagues working in theoretical particle physics, I deemed it more useful providing a description of what is the current activity of the physicists working in solar neutrinos, leaving the listener free of forming his/her own opinion apropos.
Probing hot gas around luminous red galaxies through the Sunyaev–Zel’dovich effect

We construct the mean thermal Sunyaev–Zel’dovich (tSZ) Comptonization y-profile around luminous red galaxies (LRGs) in the redshift range 0.16 < z < 0.47 from the Sloan Digital Sky Survey Data Release 7 using the Planck y-map. We detect a significant tSZ signal out to ∼30 arcmin, which is well beyond the 10 arcmin angular resolution of the y-map and well beyond the virial radii of the LRGs. We compare the measured profile with predictions from the cosmo-OWLS suite of cosmological hydrodynamical simulations. The best agreement is obtained for models that include efficient feedback from active galactic nuclei, over and above feedback associated with star formation. We also compare our results with predictions based on the halo model with a universal pressure profile giving the y-signal. The predicted profile is consistent with the data when using stacked weak lensing measurements to estimate the halo masses of the LRGs, but only if we account for the clustering of neighbouring haloes via a two-halo term.