Miracle-dz/UniFAR-FTUs huggingface dataset:
Digital Versus Conventional Full-Arch Implant Impressions: A Prospective Study on 16 Edentulous Maxillae.

PURPOSE A prospective clinical study to compare for the first time the accuracy of digital and conventional maxillary implant impressions for completely edentulous patients. MATERIALS AND METHODS Sixteen patients received maxillary implant supported fixed complete dentures. After the verification of the conventional final casts, the casts were scanned with a desktop (extraoral) scanner. Intraoral full-arch digital scans were also obtained with scan bodies and STL files from both extraoral and intraoral scans were superimposed and analyzed with reverse engineering software. The primary outcome measure was the assessment of accuracy between scans of the verified conventional casts and digital full-arch impressions. The secondary outcome was the effect of the implant number on the 3D accuracy of impressions with Spearman's rank correlation coefficient. RESULTS The 3D deviations between virtual casts from intraoral full-arch digital scans and digitized final stone casts generated from conventional implant impressions were found to be 162 ±77 μm. In the 4-implant group, 5-implant group, and 6-implant group the 3D deviations were found to be 139 ±56 μm, 146 ±90 μm, and 185 ±81 μm, respectively. There was a positive correlation between increased implant number and 3D-deviations, but there was no statistically significant difference (P = 0.191). CONCLUSIONS The 3D accuracy of full-arch digital implant scans lies within previously reported clinically acceptable threshold. Full-arch digital scans and a complete digital workflow in the fabrication of maxillary fixed complete dentures may be clinically feasible. This article is protected by copyright. All rights reserved.
Cockle Shell-Derived Aragonite CaCO3 Nanoparticles for Co-Delivery of Doxorubicin and Thymoquinone Eliminates Cancer Stem Cells

Cancer stem cells CSCs (tumour-initiating cells) are responsible for cancer metastasis and recurrence associated with resistance to conventional chemotherapy. This study generated MBA MD231 3D cancer stem cells enriched spheroids in serum-free conditions and evaluated the influence of combined doxorubicin/thymoquinone-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles. Single loaded drugs and free drugs were also evaluated. WST assay, sphere forming assay, ALDH activity analysis, Surface marker of CD44 and CD24 expression, apoptosis with Annexin V-PI kit, cell cycle analysis, morphological changes using a phase contrast light microscope, scanning electron microscopy, invasion assay and migration assay were carried out; The combination therapy showed enhanced apoptosis, reduction in ALDH activity and expression of CD44 and CD24 surface maker, reduction in cellular migration and invasion, inhibition of 3D sphere formation when compared to the free drugs and the single drug-loaded nanoparticle. Scanning electron microscopy showed poor spheroid formation, cell membrane blebbing, presence of cell shrinkage, distortion in the spheroid architecture; and the results from this study showed that combined drug-loaded cockle-shell-derived aragonite calcium carbonate nanoparticles can efficiently destroy the breast CSCs compared to single drug-loaded nanoparticle and a simple mixture of doxorubicin and thymoquinone.
Association of Neisseria gonorrhoeae Plasmids With Distinct Lineages and The Economic Status of Their Country of Origin

Abstract Plasmids are vehicles for horizontal gene transfer between bacteria, and in Neisseria gonorrhoeae plasmids can mediate high-level antimicrobial resistance (AMR). Using genomic and phylogenetic analyses, we show that plasmids are widespread in a collection of 3724 gonococcal isolates from 56 countries, and characterized the conjugative, β-lactamase and cryptic plasmids. We found that variants of the conjugative plasmid (which can mediate tetracycline resistance) and the β-lactamase plasmid expressing TEM-135 are associated with distinct gonococcal lineages. Furthermore, AMR plasmids are significantly more prevalent in gonococci from less wealthy countries, highlighting the need for further studies. More than 94% of gonococci possess the cryptic plasmid, with its absence correlated with the presence of a novel chromosomal type IV secretion system. Our results reveal the extent of plasmid-mediated AMR in the gonococcus, particularly in less wealthy countries, where diagnostic and therapeutic options can be limited, and highlight the risk of their global spread.
Factors that Influence Chinese Parents’ Intentions to Use Physical Violence to Discipline Their Preschool Children

This study explored factors affecting parents’ intentions to use physical violence (PV) to discipline their children in the future. The theory of planned behavior (TPB) guided selection of variables. A sample of 1337 preschool children’s parents from nine kindergartens located in a county of Henan Province, China were selected by stratified random cluster sampling. Data on parents’ attitudes, subjective norms, and perceived behavioral control over PV, intentions to engage in PV to discipline their preschool children in the future, self-reported PV behavior toward their children during the past three months, and demographic characteristics were collected via a paper-based questionnaire. Multivariable logistic regression analyses examined putative predictors of parents’ intentions to use physically violent discipline. Nearly three-quarters of the sample said they definitely will not use violent discipline, while 23.4% either said they would use it, or did not rule it out. Logistic regression analysis showed that parents’ lower level of perceived behavioral control over using violence (OR 4.17; 95% CI: 2.659, 6.551), attitudes that support PV (OR 2.23; 95% CI: 1.555, 3.203), and having been physically violent with their children during the past three months (OR 1.62; 95% CI: 1.032, 2.556) were significantly associated with parents’ tendency either to include, or not exclude, the use of violent discipline. Parents’ subjective norms regarding PV had no significant impact on their intentions (p > 0.05). The influence of TPB constructs varied according to parents’ gender. Intervention programs that aim to reduce violent discipline should focus both on increasing parents’ perceived behavioral control over PV and changing their attitudes toward physically violent practices, especially among mothers and parents who have already used PV to discipline their children.
Seasonal variation in vitamin D status, bone health and athletic performance in competitive university student athletes: a longitudinal study

Vitamin D deficiency has been commonly reported in elite athletes, but the vitamin D status of UK university athletes in different training environments remains unknown. The present study aimed to determine any seasonal changes in vitamin D status among indoor and outdoor athletes, and whether there was any relationship between vitamin D status and indices of physical performance and bone health. A group of forty-seven university athletes (indoor n 22, outdoor n 25) were tested during autumn and spring for serum vitamin D status, bone health and physical performance parameters. Blood samples were analysed for serum 25-hydroxyvitamin D (s-25(OH)D) status. Peak isometric knee extensor torque using an isokinetic dynamometer and jump height was assessed using an Optojump. Aerobic capacity was estimated using the Yo-Yo intermittent recovery test. Peripheral quantitative computed tomography scans measured radial bone mineral density. Statistical analyses were performed using appropriate parametric/non-parametric testing depending on the normality of the data. s-25(OH)D significantly fell between autumn (52·8 (sd 22·0) nmol/l) and spring (31·0 (sd 16·5) nmol/l; P < 0·001). In spring, 34 % of participants were considered to be vitamin D deficient (<25 nmol/l) according to the revised 2016 UK guidelines. These data suggest that UK university athletes are at risk of vitamin D deficiency. Thus, further research is warranted to investigate the concomitant effects of low vitamin D status on health and performance outcomes in university athletes residing at northern latitudes.
Gender differences in Community-acquired Pneumonia.

Community-acquired pneumonia (CAP) is the most common type of lower respiratory tract infection and a major cause of morbidity and mortality in adults worldwide. Sex and gender play an active role in the incidence and outcomes of major infectious diseases, including CAP. Some studies have reported higher male susceptibility to pulmonary infections and higher risk of death due to sepsis, but others have found the opposite effect. Biological differences (e.g. hormonal cycles and cellular immune- mediated responses) together with cultural, behavioral and socio-economic differences are important determinants of the course and outcome of CAP. Identify gender differences in CAP can guide patient's prognostication and management. The purpose of this review is to provide the available literature evidences about gender's influence on the clinical course of CAP in order to optimize the assessment, monitoring and treatment of this disease and to reduce its negative outcomes.
Critical closing pressure during experimental intracranial hypertension: comparison of three calculation methods

ABSTRACT Objectives: The critical closing pressure (CrCP) defines arterial blood pressure below which cerebral arteries collapse. It represents a clinically relevant parameter for the estimation of cerebrovascular tone. Although there are few methods to assess CrCP, there is no consensus which of them estimates this parameter most accurately. The aim of present retrospective, experimental study was to compare three methods of CrCP estimation: conventional Aaslid’s formula and methods based on the cerebrovascular impedance: the established continuous flow forward (CFF) and a new pulsatile flow forward (PFF) model. Methods: The effects of the following physiological manoeuvres on the CrCP were studied in New Zealand white rabbits: lumbar infusion of Hartmann’s solution to induce mild intracranial hypertension, sympathetic blockade to induce arterial hypotension, and modulation of respiratory tidal volume to induce hypocapnia or hypercapnia. Results: During intracranial hypertension, all CrCP estimates were significantly higher than at baseline, decreased with decreasing ABP and increased with gradual hypocapnia. During hypercapnia, all CrCP estimates were significantly decreased but only in the case of CrCPA the negative, non-physiological values were observed (16% of the cases). The Bland–Altman analysis revealed that a good agreement between each impedance method and Aaslid’s method deteriorated significantly in the low range of the average numerical value of the estimates. Discussion: Our results confirm the limited usage of Aaslid’s formula for the calculation of CrCP. Although both impedance methods seem to be equivalent, the fact that PFF model better describes cerebrovascular hemodynamic allows the recommendation of this model for the calculation of CrCP.
Relationships Between External- and Internal-Workload Variables in an Elite Female Netball Team and Between Playing Positions.

PURPOSE To examine relationships between external- and internal-workload variables in an elite female netball team, with consideration of positional differences. METHODS Nine elite female netball athletes had their weekly workloads monitored across their preseason and competition phases of a season. Internal workload was determined using summated heart-rate (HR) zones and session ratings of perceived exertion (sRPE), whereas external workload was determined using inertial movement units and included absolute PlayerLoad (PL), relative PL (PL per minute), accelerations (ACCEL), decelerations (DECEL), jumps, changes of direction (COD), high-intensity events, medium-intensity events, low-intensity events, PL in the forward direction, PL in the sideways direction, and PL in the vertical direction. Relationships between external- and internal-workload variables in the team and relative to playing position were examined. RESULTS Across the team, the strongest external workloads that correlated with summated HR zones were PL (r = .65), COD (r = .64), ACCEL (r = .61), and DECEL (r = .61). The strongest external workloads that correlated with sRPE were COD (r = .79), followed by jumps (r = .76), ACCEL (r = .75), and DECEL (r = .75). For all positions, except-goal shooter, the strongest correlation was between PL and sRPE (r = .88-.94). In the goal-shooter position, the strongest correlation was between summated HR zones and DECEL (r = .89). CONCLUSIONS The inertial movement unit-derived external-workload variables are strongly related to common internal-workload variables. In particular, COD and sRPE appear to provide a good monitoring combination of external and internal training loads for elite netball players.
Comparison between semitendinosus transfer to distal femur and medial hamstrings surgical lengthening for treatment of flexed knee gait in cerebral palsy

Purpose: Hamstrings surgical lengthening (HSL) has been frequently used for the treatment of flexed knee gait in cerebral palsy; however, recurrence of knee flexion deformity (KFD) and increase of anterior pelvic tilt (APT) were reported in a long-term follow-up. Research question: The aim of this study was to compare semitendinosus transfer to distal femur (STTX) and semitendinosus surgical lengthening (STL) regarding the reduction of KFD and the increase of APT after flexed knee gait treatment. Methods: One hundred and eleven patients were evaluated and they were divided into two groups according to surgical procedures at knees: group A (65 patients/130 knees), including patients who received medial HSL as part of multilevel approach; group B (46 patients/92 knees), represented by patients who underwent orthopedic surgery including an STTX instead of STL. Results: Fixed knee flexion deformity (FKFD) decreased only in group B (from 6.79° to 2.96°, p < 0.001) after intervention. In kinematics, APT increased from 16.38° to 19.03° in group A (p = 0.003), while group B also increased from 15.26° to 20.59° (p < 0.001). The minimum knee flexion in stance phase (MKFS) reduced from 25.34° to 21.65° (p = 0.016) in group A and from 31° to 19.57° (p < 0.001) in group B. In the comparison between groups A and B, the increase of APT (p = 0.028) and reduction of FKFD (p < 0.001), popliteal angle (p = 0.001), bilateral popliteal angle (p = 0.003) and MKFS (p = 0.006) were higher after STTX than STL. Conclusion: In the present study, patients who received STTX exhibited more improvement of knee extension at clinical examination and during gait than those who underwent to STL; however, STTX was not effective to prevent the increase of APT after flexed knee gait treatment.
Lost in Translation: Physiological Roles of Stored mRNAs in Seed Germination

Seeds characteristics such as germination ability, dormancy, and storability/longevity are important traits in agriculture, and various genes have been identified that are involved in its regulation at the transcriptional and post-transcriptional level. A particularity of mature dry seeds is a special mechanism that allows them to accumulate more than 10,000 mRNAs during seed maturation and use them as templates to synthesize proteins during germination. Some of these stored mRNAs are also referred to as long-lived mRNAs because they remain translatable even after seeds have been exposed to long-term stressful conditions. Mature seeds can germinate even in the presence of transcriptional inhibitors, and this ability is acquired in mid-seed development. The type of mRNA that accumulates in seeds is affected by the plant hormone abscisic acid and environmental factors, and most of them accumulate in seeds in the form of monosomes. Release of seed dormancy during after-ripening involves the selective oxidation of stored mRNAs and this prevents translation of proteins that function in the suppression of germination after imbibition. Non-selective oxidation and degradation of stored mRNAs occurs during long-term storage of seeds so that the quality of stored RNAs is linked to the degree of seed deterioration. After seed imbibition, a population of stored mRNAs are selectively loaded into polysomes and the mRNAs, involved in processes such as redox, glycolysis, and protein synthesis, are actively translated for germination.
Emergence of CTX-M-27-producing Escherichia coli of ST131 and clade C1-M27 in an impacted ecosystem with international maritime traffic in South America

3 Xie M, Li R, Liu Z et al. Recombination of plasmids in a carbapenemresistant NDM-5-producing clinical Escherichia coli isolate. J Antimicrob Chemother 2018; 73: 1230–4. 4 Li R, Chen K, Chan EWC et al. Resolution of dynamic MDR structures among the plasmidome of Salmonella using MinION singlemolecule, long-read sequencing. J Antimicrob Chemother 2018; 73: 2691–5. 5 Kolmogorov M, Yuan J, Lin Y et al. Assembly of long, error-prone reads using repeat graphs. Nat Biotechnol 2019; 37: 540–6. 6 Toleman MA, Walsh TR. ISCR elements are key players in IncA/C plasmid evolution. Antimicrob Agents Chemother 2010; 54: 3534. 7 Toleman MA, Bennett PM, Walsh TR. ISCR elements: novel gene-capturing systems of the 21st century? Microbiol Mol Biol Rev 2006; 70: 296–316. 8 Partridge SR. Analysis of antibiotic resistance regions in Gram-negative bacteria. FEMS Microbiol Rev 2011; 35: 820–55. 9 He T, Wang R, Liu D et al. Emergence of plasmid-mediated high-level tigecycline resistance genes in animals and humans. Nat Microbiol 2019; 4: 1450–6.
The impact of long-term moderate level of vaccination coverage for epidemiology of varicella in Lu'an, China: should we change immunisation strategy now?

Abstract As China implements the voluntary vaccination programme of one-dose of varicella vaccine (VarV) for decades, robust estimates of the impact of voluntary vaccination era on epidemiology of varicella are needed. We estimated the vaccination coverage (VC) of VarV by using surveillance data on immunisation. The descriptive epidemiological method was used to describe the changing epidemiology of varicella from 2007 to 2018. The screening method was used to estimate the vaccine effectiveness (VE) of VarV. The overall VC for VarV was 71.7%, ranged from 47.7% to 79.5% among 2008–2017 birth cohorts. In total, 16 660 varicella cases were reported during 2007–2018, the incidence increased from 10.0 cases per 100 000 population in 2007 to 65.2 cases per 100 000 population in 2018. A shift in age group of varicella was observed since 2012, with the age increased from 5–9 years to 10–14 years. The overall VE was 79.9%, and the VE increased from 60.1% in 2008 birth cohort to 96.2% in 2017 birth cohort. We found that the overall VE for VarV is moderate, but appears highly effective within 5 years after vaccination. In addition, a shift varicella infection to older ages has occurred at the long-term moderate level VC of one-dose VarV. Therefore, to contain the incidence of varicella and prevent any potential shift to older ages, the introduction of VarV into routine immunisation programme is likely needed in Lu'an.
Assessment of motion ranges of knee and ankle joints of the affected limbs in patients with hemiparesis after cerebrovascular accidents

Wstep. Udar mozgu jest jednym z najpowazniejszych problemow zdrowotnych spoleczenstw z krajow uprzemyslowionych. Chory konczący wczesną rehabilitacje poudarową, czyli 2-3 miesiące od udaru powinien chodzic samodzielnie lub z niewielką pomocą. C e l e m p r a c y byla analiza skuteczności wczesnej specjalistycznej rehabilitacji poudarowej, realizowanej metodą PNF, w oparciu o analize zakresu ruchomości stawu kolanowego i skokowego konczyny niedowladnej u osob po mozgowym incydencie naczyniowym. M a t e r i a l i m e t o d y. Badaniem objeto 30 osob po mozgowym incydencie naczyniowym poddanych wczesnej rehabilitacji metodą PNF na pododdziale udarowym. Grupe badawczą stanowilo 13 mezczyzn i 17 kobiet, o średniej wieku ± 66,1. Czas przebywania chorego na oddziale wynosil średnio 28 dni. Pierwsze badanie wykonano przed rozpoczeciem usprawniania w dniu przyjecia chorego, a kontrolne w dniu wypisu z oddzialu udarowego. W y n i k i. Stwierdzono wyraźną redukcje deficytow w stawie kolanowym i skokowym konczyny niedowladnej. W badaniu drugim wykazano znaczny wzrost zakresu ruchu zginania w konczynie niedowladnej. Wartośc ruchu wyprostu pozostala bez zmian. Grupa chorych z niedowladem lewostronnym charakteryzowala sie znacznie wiekszymi ograniczeniami zakresow poszczegolnych ruchow czynnych stawu kolanowego oraz skokowego gornego i dolnego niz grupa chorych z niedowladem prawostronnym. W n i o s k i. Stosowanie metody PNF we wczesnej rehabilitacji wyraźnie usprawnilo funkcjonowanie konczyny niedowladnej chorych po przebytym mozgowym incydencie naczyniowym.
Biosynthesis of silver nanoparticles by Fusarium scirpi and its potential as antimicrobial agent against uropathogenic Escherichia coli biofilms

The ability of Uropathogenic Escherichia coli (UPEC) to form biofilms, can be considered an important factor that contributes to the prevalence of Urinary Tract Infections (UTIs) due to the inaccessibility of the antibiotics into the highly complex structure of the biofilm. Moreover, with the appearance of antibiotic multiresistant UPEC strains, the alternatives of treatment of UTIs are less. Silver nanoparticles (AgNPs) can be useful in the treatment of the UPEC infections due to its physicochemical properties that confer them antibacterial activity against both planktonic and biofilm structured cells. A diversity of biological methods for synthesis of AgNPs with antimicrobial activity has been widely investigated during the last decades, between these methods; the fungal-biosynthesis of AgNPs highlights as an ecofriendly, scalable and low cost method. In this study, biogenic AgNPs were synthesized with extracellular metabolites secreted by the soil fungal strain Fusarium scirpi (Ag0.5–5) by an ecofriendly, simple and efficient method. The antimicrobial activity of the biosynthesized AgNPs against UPEC was evaluated. The Minimal Inhibitory Concentration (MIC) of biogenic AgNPs over planktonic UPEC cells was 25 mg/mL, whereas a sub-MIC concentration (7.5 mg/L) was sufficient to inhibit the UPEC-biofilm formation about a 97%, or produce the disruption of an 80% of mature UPEC-biofilms demonstrating the potential of fungal-derived AgNPs to prevent UPEC infections.