Miracle-dz/UniFAR-FTUs huggingface dataset:
Timing of Repair in Tetralogy of Fallot: Effects on Outcomes and Myocardial Health.

Early complete repair of Tetralogy of Fallot (ToF) prior to 1 year of age has been demonstrated to be safe and has survival benefits over late repair. The age at repair of ToF affects long-term outcomes. This may largely be related to preserved, or comparatively better, myocardial health. Most studies advocate for an age of repair between 3-6 months and certainly below the age of 1 year. Patients with severe right ventricular outflow tract obstruction represent an exception to this rule and may require neonatal repair or surgical and catheter-based palliation before surgery. Older age at repair beyond the first birthday leads to unfavorable right ventricular remodeling with increased right ventricular stiffness and hypertrophy, and is associated with increased long-term ventricular tachycardia and all-cause mortality. In this article, we review the short and long-term benefits of early repair, with a focus on long-term morbidity. In conclusion, we emphasize the importance of myocardial health and the relationship to early repair, and advocate for the use of magnetic resonance imaging in adult patients with repaired ToF in order to detect myocardial fibrosis.
miRNAs as radio‐response biomarkers for breast cancer stem cells

In breast cancer (BC), the presence of cancer stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but is not always effective. CSCs have several mechanisms of radioresistance in place, and some miRNAs are involved in the cellular response to ionizing radiation (IR). Here, we studied how IR affects the expression of miRNAs related to stemness in different molecular BC subtypes. Exposition of BC cells to radiation doses of 2, 4, or 6 Gy affected their phenotype, functional characteristics, pluripotency gene expression, and in vivo tumorigenic capacity. This held true for various molecular subtypes of BC cells (classified by ER, PR and HER‐2 status), and for BC cells either plated in monolayer, or being in suspension as mammospheres. However, the effect of IR on the expression of eight stemness‐ and radioresistance‐related miRNAs (miR‐210, miR‐10b, miR‐182, miR‐142, miR‐221, miR‐21, miR‐93, miR‐15b) varied, depending on cell line subpopulation and clinicopathological features of BC patients. Therefore, clinicopathological features and, potentially also, chemotherapy regimen should be both taken into consideration, for determining a potential miRNA signature by liquid biopsy in BC patients treated with RT. Personalized and precision RT dosage regimes could improve the prognosis, treatment, and survival of BC patients.
Characterizing the University of California’s tenure-track teaching position from the faculty and administrator perspectives

Teaching faculty are a potential mechanism to generate positive change in undergraduate STEM education. One such type of faculty is the Lecturer with Potential Security of Employment (L(P)SOE), a tenure-track faculty line within the University of California (UC) system. As a foundation for future studies, we sought to characterize individuals in the L(P)SOE position in terms of their background training, job expectations, and resources available for their success. Data were collected through an online survey completed by over 80% of STEM L(P)SOEs across the UC system, as well as interviews with over 20 deans and chairs in STEM departments at three UC campuses. From this work, we found that the majority of current L(P)SOEs were formally trained within their disciplines and not in an education field; however, they possessed substantial education experience, such as classroom teaching or participation in professional development opportunities. Expectations for time spent on teaching, research, and service are aligned between individuals within varying ranks of the L(P)SOE faculty and between L(P)SOEs and administrators. L(P)SOEs and administrators are also in agreement about what constitutes acceptable professional development activities. Interestingly, we identified differences that may reflect changes in the position over time, including increased start-up funds for more recently hired L(P)SOE faculty and a differing perspective on the role of discipline-based education research and scholarly activities between non-tenured and more senior L(P)SOEs. Overall, these data provide a snapshot of the L(P)SOE position that will aid in future work to identify the potential institutional impact of these individuals.
Hypermethylation of miR-34b/c is associated with early clinical stages and tumor differentiation in Kazakh patients with esophageal squamous cell carcinoma.

DNA hypermethylation in tumor suppressor genes has been reported in some cancers. The microRNA-34b/c (miR-34b/c) serves as tumor suppressors in different tumor types. To investigate the methylation status of miR-34b/c in ESCC, MALDI-TOF MS was used to quantitatively analyze the DNA methylation of 16 CpG sites within miR-34b/c in 145 ESCC samples, 60 cancer-adjacent normal (ACN) samples and 39 normal esophageal (NE) samples from the Kazakh population. Our results showed that the overall average methylation levels of miR-34b/c were significantly higher in the ESCC samples than they were in the ACN and NE samples (P < 0.05). Furthermore, the methylation levels of CpG_1. 2.3, CpG_9. 10, CpG_11. 12.13, CpG_14, and CpG_15. 16 of miR-34b/c were significantly higher in the ESCC tissues than they were in the ACN (P < 0.05) and NE tissues (P < 0.05). Additionally, the mean methylation levels at CpG_9. 10 and CpG_14 were all significantly higher in the ACN samples than they were in the NE samples (P < 0.01). Increased methylation levels of CpG_9. 10 and CpG_11. 12.13 in miR-34b/c predominantly occurred in the early stages (UICC I/II) of ESCC (P < 0.05), and the methylation differences (moderately-poorly differentiated > well differentiated) in miR-34b/c CpG_1. 2.3 were significant (P < 0.05). This is the first study reporting that the hypermethylation of miR-34b/c plays an important role in ESCC and is significantly correlated with the early stages and tumor differentiation of ESCC. The hypermethylation of miR-34b/c may promote the oncogenesis and progression of ESCC, and these findings may provide support for the future development of targeted therapies.
Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosis

As a convenient, effective and economical kidney replacement therapy for end‐stage renal disease (ESRD), peritoneal dialysis is available in approximately 11% of ESRD patients worldwide. However, long‐term peritoneal dialysis treatment causes peritoneal fibrosis. In recent years, the application potential of molecular hydrogen in the biomedicine has been well recognized. Molecular hydrogen selectively scavenges cytotoxic reactive oxygen species (ROS) and acts as an antioxidant. In this experiment, a high glucose‐induced peritoneal fibrosis mouse model was successfully established by intraperitoneal injection of high glucose peritoneal dialysate, and peritoneal fibrosis mice were treated with hydrogen‐rich peritoneal dialysate. In addition, in vitro studies of high glucose‐induced peritoneal fibrosis were performed using MeT‐5A cells. In vitro and in vivo experiments show that molecular hydrogen could inhibit peritoneal fibrosis progress induced by high glucose effectively. Furthermore, it has been found that molecular hydrogen alleviate fibrosis by eliminating intracellular ROS and inhibiting the activation of the PTEN/AKT/mTOR pathway. The present data proposes that molecular hydrogen exerts the capacity of anti‐peritoneal fibrosis through the ROS/PTEN/AKT/mTOR pathway. Therefore, molecule hydrogen is a potential, safe, and effective treatment agent, with peritoneal protective property and great clinical significance.
Internet Gaming Disorder In Students Of Peshawar: A Cross Sectional Survey.

BACKGROUND Internet gaming, though getting popular, is becoming a risk to physical and psychological health, especially in adolescents, as it contributes to sedentary lifestyle. To gauge the extent of the problem in our setup, we decided to find the frequency of internet gaming disorder in students of Peshawar and identify their motives for playing these games. METHODS This Cross-sectional survey was conducted from February to December 2018 and included students of Private and Public institutes of Peshawar. They were invited to complete a questionnaire comprising of Internet Gaming Disorder Scale-Short Form (IGDS-SF9), which includes 9 questions, with some additional demographic questions. The data were analysed using SPSS v.25. RESULTS The mean age of the sample (n=2116) was 21.5±2.1 years with the age range of 16-28 years. The cronbach's alpha reliability of IGDS-SF9 in our study was 0.805. Majority of the participants were males (n=1261, 59.6%) and were from Private sector institutes (n=1314, 62.1%). A total of 32 (1.5%) students reported having Internet Gaming disorder. The results of chi square test showed that significantly more male students played games for social motives (p=.032), whereas significantly more female students played games for coping motives (p=.001). Pearson correlation results showed a significant positive correlation between time spent on gaming and internet gaming disorder (p=.000) and between internet gaming disorder with recreation as a motive (p=.027). CONCLUSIONS Internet Gaming is getting common in our setup and IGDS-SF9 can be effectively used in our population because of its reliability.
Ibrutinib resistance in a patient with transformed diffuse large B-cell lymphoma from primary pulmonary mucosa-associated lymphoid tissue lymphoma

ABSTRACT Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) lymphoma is rare among lung neoplasia cases, representing only 0.5%–1% of newly diagnosed primary lung lymphoma. MALT lymphoma with relapsed refractory and malignant transformation is highly heterogeneous and consensus therapy remains undetermined. We report a 55 year-old woman with a 3 year history of primary pulmonary MALT lymphoma confined to the lung presenting with massive pleural effusion. After two cycles of R-CHOP and six cycles of R2-CHOP, pleural effusion disappeared but the pulmonary mass remained persistent. Second-line therapies R2-GemOx failed to make any substantial improvement. Core-needle puncture biopsy of the pulmonary mass was obtained and pathological testing revealed transformed diffuse large B-cell lymphoma of germinal center B-cell subtype. Next-generation sequencing confirmed BN2 subtype. The mass showed no reduction after three cycles of R-MINE, following which the BTK inhibitor ibrutinib was administered to this patient. Unfortunately, after two months of ibrutinib treatment, the patient rapidly developed an enlarged mass and hyperprogressive disease, to which she subsequently succumbed.
Clinical characteristics and prognostic analysis of ipsilateral supraclavicular lymph node metastases in breast cancer patients: a retrospective study.

BACKGROUND To investigate the clinical characteristics and prognosis of different subtype breast cancer patients with pathologically proven ipsilateral supraclavicular lymph node metastasis (ISCLM). METHODS We performed a retrospective analysis of clinical data for total 156 patients who diagnosed with ISCLM, among 3,133 breast cancer patients. Breast cancer subtypes were classified as hormone receptor (HR) positive (+)/human epidermal growth factor receptor 2 (HER2) negative (-), HR+/HER2+, HR-/HER2+ and triple-negative breast cancer (TNBC) subtypes using immunohistochemical staining. We subsequently analyzed matched cohorts, evaluating clinical features and survival in different subtypes. RESULTS The patients in this study accounted for 5% of all breast cancer patients treated during the same period. Breast cancer subtypes were confirmed in all 156 patients (39.7% with HR+/HER2-, 23.7% with HR+/HER2+, 14.7% with HR-/HER2+, and 21.8% with TNBC). The median overall survival after ISCLM was 47 months (95% confidence interval (CI), 3-94 months), and the overall 1-, 3- and 5-year survival rates were 92.3, 73.7 and 48.1%, respectively. We found that the survival rate is impacted by breast cancer subtypes (P = 0.001), and patients with TNBC had the shortest survival. Time to ISCLM less than 24 months and ISCLM size > 3 cm were independent predictors of poor survival in patients with ISCLM (P = 0.007 and 0.001, respectively). CONCLUSIONS Clinical breast cancer subtypes were still independent prognostic predictors among breast cancer patients with ISCLM. ISCLM arising from TNBC has the worst prognosis. Multidisciplinary therapy is beneficial for breast cancer patients with ISCLM.
Mentalizing in mothers and children with type 1 diabetes

Abstract Studies suggest that the relationship between psychosocial well-being and type 1 diabetes (T1D) is bidirectional, with T1D typically having a negative influence on psychological functioning, which in turn negatively affects the course of T1D. Here, we investigate the potential role of the capacity for mentalizing, or reflective functioning, in children and their mothers in diabetes control. We tested differences in mentalizing as assessed by the Reflective Functioning Scale in two groups of mother–son dyads with good (GDC) versus poor (PDC) diabetes control. Fifty-five boys (8–12 years old) and their mothers were recruited from the Juvenile Diabetes Foundation in Santiago, Chile. The mothers were interviewed with the Parental Development Interview and the children with the Child Attachment Interview, and both were scored for reflective functioning by using the Reflective Functioning Scale. Self-report measures of stress and diabetes outcomes were completed by the mothers and children, and levels of glycated hemoglobin (HbA1c) were assessed as an index of diabetes control. The results showed that both maternal and child reflective functioning were higher in the GDC than the PDC group and were negatively correlated with HbA1c in the total sample. Our findings suggest an important role for mentalizing in diabetes outcomes, but further prospective research is needed.
Hsa_circ_0086414 Might Be a Diagnostic Biomarker of Oral Squamous Cell Carcinoma

Background Circular RNAs (circRNAs), a newly-discovered class of non-coding RNAs, have a significant role in the progression of cancers, but the effect of hsa_circ_0086414 in human oral squamous cell carcinoma (OSCC) is still unclear. Material/Methods The circRNAs expression profile in OSCC tissue samples was assessed by high-throughput sequencing. The hsa_circ_0086414 expression level in 55 paired OSCC tissue samples and 2 kinds of OSCC cells was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the correlation between the hsa_circ_0086414 expression and clinicopathological characteristics of individuals with OSCC was studied. We used receiver operating characteristic (ROC) curves to observe the hsa_circ_0086414 value of diagnosis in OSCC. The network of hsa_circ_0086414-miRNAs-mRNAs was constructed. Gene Ontology (GO), Disease Oncology (DO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out based on sequencing data and bioinformatics predictions. Results Hsa_circ_0086414 expression in OSCC tissue samples and OSCC cells was first discovered to be significantly downregulated compared with the adjacent healthy tissues (AHTs) and normal (HaCaT) cells, respectively. Moreover, its expression level was significantly correlated with stage in TNM, size of tumor, and lymph node metastasis. The area below the ROC curve was 0.749. Hsa_circ_0086414-miRNAs-mRNAs network analysis and GO, DO, and KEGG analyses all demonstrated that hsa_circ_0086414 is correlated with cancer progression to a certain extent. Conclusions We discovered that hsa_circ_0086414 might be an essential diagnostic biomarker in OSCC. Furthermore, hsa_circ_0086414 could be a target for OSCC therapy.
MiR-197-3p regulates endothelial cell proliferation and migration by targeting IGF1R and BCL2 in Kawasaki disease.

BACKGROUND Kawasaki disease (KD) is a multisystemic vasculitis syndrome. Accumulating evidences indicated that microRNAs play a critical role in KD. However, the mechanism was still not fully understood. The study aimed to research the functions of microRNA-197-3p (miR-197-3p) in the progression of KD. METHODS Level of miR-197-3p was detected by quantitative polymerase chain reaction (qRT-PCR) in acute KD serum. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was performed to determine cell proliferation in HCAECs. Cell apoptosis was evaluated by flow cytometry. In addition, transwell assay was used to identify the migration capacity of HCAECs in vitro. The expression of insulin-like growth factor type 1 receptor (IGF1R), B cell lymphoma 2 (BCL2), apoptosis-relative of Cleaved-caspase 3 (C-caspase 3) and Cleaved-PARP (C-PARP), as well as transition-relative of E-cadherin, N-cadherin, and Vimentin were measured by western blot assay. Lastly, dual-luciferase reporter assay was employed to verify the relationship between miR-197-3p and IGF1R or BCL2 in vitro. RESULTS Level of miR-197-3p was higher in Acute KD samples than that of healthy control and convalescent KD samples. Mechanistically, the role of miR-197-3p was exerted through directly targeting IGF1R and BCL2 in KD serum-induced HCAECs. Functionally, the inhibiting effect on cell apoptosis as well as promoting effects on cell proliferation and migration of miR-197-3p deletion was abrogated by either si-IGF1R or si-BCL2. CONCLUSION miR-197-3p modifies cell behaviors of proliferation, apoptosis and migration by targeting IGF1R and BCL2 in KD, providing a new perspective for the treatment of KD clinically.
The Effect of High-Intensity Interval Training on Physical Parameters, Metabolomic Indexes and Serum Ficolin-3 Levels in Patients with Prediabetes and Type 2 Diabetes

Abstract Background Exercise benefits people with abnormal glucose metabolism, and serum ficolin-3 levels have been reported to be associated with diabetes. However, no relevant study has discussed the relationship between high-intensity interval training (HIIT) and ficolin-3 in a Chinese population. Objective To compare the effect of HIIT and moderate-intensity continuous training (MICT) on blood pressure, glucose control, the lipid profile and the serum ficolin-3 level in patients with prediabetes and type 2 diabetes (T2D). Methods We recruited 145 patients with prediabetes and 196 T2D patients from March to June 2018. All participants were randomly grouped into HIIT and MICT groups. HIIT consisted of progressing to twelve 1-min bouts at 90% maximal aerobic capacity (1 min recovery), and MICT consisted of progressing to 20 min at 65% maximal aerobic capacity. ˙VO2peak, body composition, blood pressure, glucose, the lipid profile and the serum ficolin-3 level were measured before and after three weeks of training. Results After 3 weeks of training, participants in both the HIIT and MICT groups had significantly lower SBP, BMI, waist circumference, % body fat, and serum levels of FPG, TC, TGs, UA and ficolin-3, as well as increased vital capacity and VOmax. Additionally, the patients in the HIIT group still had significantly lower levels of 2hPG and LDL-C, regardless of prediabetes or diabetes status. After comparing the differences in the variation in parameters between the HIIT and MICT groups, we found that HIIT could help patients with prediabetes or diabetes acquire better effects of treatment in regard to anthropometry, blood pressure, glucose control, UA and ficolin-3 levels than MICT. Finally, the patients in the HIIT group had a lower rate of loss to follow-up and a higher rate of session attendance. Conclusions Both HIIT and MICT were beneficial exercise strategies for health in patients with prediabetes or T2D. However, HIIT is a more time-efficient strategy and could lower the serum level of ficolin-3 in patients after 3 weeks of training.
Eribulin monotherapy in heavily pretreated metastatic breast cancer patients in real life

Background and Aim: Our retrospective, single-center study aimed at evaluating the efficacy and safety of eribulin in heavily pretreated metastatic breast cancer (MBC) in routine clinical practice. Subjects and Methods: Twenty-eight patients treated with eribulin for MBC between May 2014 and November 2017 were included in our study. Clinical and biological assessment of toxicity was controlled at each visit. Tumor response was evaluated every three cycles of treatment. Results:Median age at eribulin treatment was 52.5-year. Tumors were hormone receptor positive (71.4%), HER2-positive (10.7%), and triple negative (TN) (25%). Most of the patients (92.8%) presented with visceral metastases, mainly in the lymph nodes (57.1%) and liver (53.6%). Median previous metastatic chemotherapy line was 4 [1–7]. Median number of metastatic sites were 3 (1–4). Median number of eribulin cycles was 4. At the end of follow-up period, 36% of the patients were still alive. Eighteen patients died due to disease progression. The objective response rate was 21.5% with a 42.9% clinical benefit rate. Median progression-free survival and overall survival (OS) were 4 (95% CI: 2.7–5.2) and 14 (95% CI: 11.8–16.1) months, respectively. Treatment was well tolerated. None of the patients discontinued eribulin treatment due to toxicity. The most commonly reported toxicities were asthenia (71.4%), peripheral neuropathy (67.9%), and neutropenia (46.4%). Conclusion: Eribulin is an effective new treatment option in heavily pretreated MBC, with a manageable toxicity profile. Our results confirm that treatment with eribulin is feasible and safe in real-world patients.
Effectiveness of Enhanced External Counterpulsation Treatment on Symptom Burden, Medication Profile, Physical Capacity, Cardiac Anxiety, and Health-Related Quality of Life in Patients With Refractory Angina Pectoris

Background Patients with refractory angina pectoris experience recurrent symptoms that limit their functional capacity, including psychological distress and impaired health-related quality of life (HRQoL), despite optimized medical therapy. Enhanced external counterpulsation (EECP) is an evidence-based alternative noninvasive treatment. Although physical well-being and mental well-being are equally important components of health, few studies have investigated the psychological effects of EECP in patients with refractory angina pectoris. Objective The aim of this study was to evaluate the effects of EECP treatment in patients with refractory angina pectoris regarding medication profile, physical capacity, cardiac anxiety, and HRQoL. Methods This quasi-experimental study with 1-group pretest-posttest design includes a 6-month follow-up of 50 patients (men, n = 37; mean age, 65.8 years) who had undergone 1 EECP course. The following pretreatment and posttreatment data were collected: medication use, 6-minute walk test results, functional class according to the Canadian Cardiovascular Society, and self-reported (ie, questionnaire data) cardiac anxiety and HRQoL. In addition, the questionnaires were also completed at a 6-month follow-up. Results After EECP treatment, patients used significantly less nitrates (P < .001), walking distance increased on average by 46 m (P < .001), and Canadian Cardiovascular Society class improved (P < .001). In addition, all but 1 subscale of cardiac anxiety and all HRQoL components improved significantly (P < .05). The positive effects for cardiac anxiety and HRQoL were maintained at the 6-month follow-up. Conclusions Enhanced external counterpulsation treatment resulted in reduced symptom burden, improved physical capacity, and less cardiac anxiety, leading to increased physical activity and enhanced life satisfaction for patients with refractory angina pectoris. Enhanced external counterpulsation treatment should be considered to improve the life situation for these patients.