Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact of a surgical ward breakfast buffet on nutritional intake in postoperative patients: A prospective cohort pilot study

Background An early return to normal intake and early mobilization enhances postoperative recovery. However, one out of six surgical patients is undernourished during hospitalization and approximately half of the patients eat 50% or less of the food provided to them. We assessed the use of newly introduced breakfast buffets in two wards for gastrointestinal and oncological surgery and determined the impact on postoperative protein and energy intake. Methods A prospective pilot cohort study was conducted to assess the impact of the introduction of breakfast buffets in two surgical wards. Adult patients had the opportunity to choose between an attractive breakfast buffet and regular bedside breakfast service. Primary outcomes were protein and energy intake during breakfast. We asked patients to report the type of breakfast service and breakfast intake in a diary over a seven-day period. Prognostic factors were used during multivariable regression analysis. Results A total of 77 patients were included. The median percentage of buffet use per patient during the seven-day study period was 50% (IQR 0–83). Mean protein intake was 14.7 g (SD 8.4) and mean energy intake 332.3 kcal (SD 156.9). Predictors for higher protein intake included the use of the breakfast buffet (β = 0.06, p = 0.01) and patient weight (β = 0.13, p = 0.01). Both use of the breakfast buffet (β = 1.00, p = 0.02) and Delirium Observation Scale scores (β = -246.29, p = 0.02) were related to higher energy intake. Conclusion Introduction of a breakfast buffet on a surgical ward was associated with higher protein and energy intake and it could be a promising approach to optimizing such intake in surgical patients. Large, prospective and preferably randomized studies should confirm these findings.
Germline Pathogenic Variants Impact Clinicopathology of Advanced Lung Cancer.

BACKGROUND The genetic factors that modulate risk for developing lung cancer have not been fully defined. Here, we sought to determine the prevalence and clinical significance of germline pathogenic/likely pathogenic variants (PV) in patients with advanced lung cancer. METHODS We studied clinical and tumor characteristics of germline PV in 5118 patients who underwent prospective genomic profiling using paired tumor-normal tissue samples in 468 cancer genes. RESULTS Germline PV in high/moderate penetrance genes were observed in 222 (4.3%) patients; of these, 193 patients had PV in DNA damage repair (DDR) pathway genes including BRCA2 (n=54), CHEK2 (n=30) and ATM (n=26) that showed high rate of biallelic inactivation in tumors. BRCA2 heterozygotes with lung adenocarcinoma were more likely to be never smokers and had improved survival compared to non-carriers. Fourteen patients with germline PV in lung cancer predisposing genes (TP53, EGFR, BAP1 and MEN1) were diagnosed at younger age compared to non-carriers, and of tumor suppressors, 75% demonstrated bi-allelic inactivation in tumors. Significantly higher proportion of germline PV in high/moderate penetrance genes were detected in high-risk patients who had either a family history of any cancer, multiple primary tumors or early age at diagnosis compared to unselected patients (10.5% vs 4.1%; p-value=1.7e-04). CONCLUSIONS These data underscore the biological and clinical importance of germline mutations in highly penetrant DDR genes as a risk factor for lung cancer. IMPACT The family members of lung cancer patients harboring PV in cancer predisposing genes should be referred for genetic counseling and may benefit from proactive surveillance.
Characteristics and Treatment Strategy of Isolated Calf Deep Venous Thrombosis after Fractures: A Review of Recent Literature

Isolated calf deep venous thrombosis (ICDVT) includes thrombosis located at the far end of the popliteal vein, such as the anterior tibial vein, posterior tibial vein, fibular vein, and intramuscular vein of the soleus and gastrocnemius. This type of thrombosis has the highest incidence, accounting for approximately half of all deep vein thrombosis (DVT) cases; however, there is no consistent recommendation for ICDVT treatment across countries, and there is also no optimal management strategy. In recent years, increasing evidence has shown that ICDVT can develop into proximal DVT, even causing pulmonary embolism (PE). Therefore, some experts suggest anticoagulant therapy for this type of DVT, while others hold an opposing attitude. Therefore, the treatment strategy for this type of DVT has become a hot and difficult research topic. The purpose of this review is to summarize the characteristics of ICDVT and the effects of different treatment strategies by analyzing recent and important classical works in the literature in an attempt to provide recommendations for the treatment of this most common type of DVT in orthopaedic clinics.
Small-molecule IKKβ activation modulator (IKAM) targets MAP3K1 and inhibits pancreatic tumor growth

Significance Pancreatic cancer is a deadly disease with limited therapeutic options. High expression of the kinase MAP3K1 is associated with poor survival among pancreatic cancer patients. MAP3K1 activates the kinase IKKβ, and our studies show that higher levels of activated IKKβ are found in patient-derived tumors as opposed to the surrounding normal tissue. We also show that inhibition of MAP3K1 by a drug-like compound 39-100 results in reduced levels of activated IKKβ. This leads to reduced tumor growth and reduced metastasis in pancreatic cancer models. Therefore, drug candidates like 39-100, that are IKKβ activation modulators, can be developed to treat pancreatic cancer.
Adult and Adolescent Disclosures of Child Sexual Abuse: A Comparative Analysis

The recent attention focused on child sexual abuse (CSA) disclosure pathways has highlighted complex psychological processes that influence disclosure both for children and adults. Some authors have suggested that such processes may differ between children and adults yet few studies have examined distinct samples within the same study. This paper addresses this gap by exploring adolescent (n = 20) and adult (n = 10) experiences of disclosure of childhood sexual abuse. Interviews were conducted with both samples, using the same interview schedule and a comparative analysis was conducted of the key themes identified from a grounded theory analysis. Those themes that were found to be common to both samples included pressure cooker effect, telling would make it worse, and self-blame. Themes that were found to be more prevalent in the adolescent sample included police/court involvement, concern for other children, being asked, and peer influence. It is suggested that such potential differences reflect the changing social context over the past few decades which is characterised by increased awareness of sexual abuse as a crime and the risks of recidivism of offenders.
Perception and Navigation in Autonomous Systems in the Era of Learning: A Survey

Autonomous systems possess the features of inferring their own state, understanding their surroundings, and performing autonomous navigation. With the applications of learning systems, like deep learning and reinforcement learning, the visual-based self-state estimation, environment perception, and navigation capabilities of autonomous systems have been efficiently addressed, and many new learning-based algorithms have surfaced with respect to autonomous visual perception and navigation. In this review, we focus on the applications of learning-based monocular approaches in ego-motion perception, environment perception, and navigation in autonomous systems, which is different from previous reviews that discussed traditional methods. First, we delineate the shortcomings of existing classical visual simultaneous localization and mapping (vSLAM) solutions, which demonstrate the necessity to integrate deep learning techniques. Second, we review the visual-based environmental perception and understanding methods based on deep learning, including deep learning-based monocular depth estimation, monocular ego-motion prediction, image enhancement, object detection, semantic segmentation, and their combinations with traditional vSLAM frameworks. Then, we focus on the visual navigation based on learning systems, mainly including reinforcement learning and deep reinforcement learning. Finally, we examine several challenges and promising directions discussed and concluded in related research of learning systems in the era of computer science and robotics.
Google Searches for Thyroid Eye Disease After Regulatory Approval of Teprotumumab.

Importance Given that direct-to-consumer advertising campaigns have the potential to both positively and negatively affect patient care, clinicians would benefit from being conscious of changes in public interest that result from such campaigns. Objective To understand how direct-to-consumer advertising was associated with information-seeking behavior based on Google Trends. Design, Setting, and Participants This descriptive cross-sectional study used Google Trends to examine relative search volumes for thyroid eye disease (TED) from January 1, 2004, to June 30, 2021, in the US. The data for this cross-sectional study were downloaded on October 17, 2021. This study included internet search engine queries originating within the US, as determined by Google Trends. Main Outcomes and Measures Monthly changes in relative search volumes for the terms "thyroid eye disease," "Tepezza," and "teprotumumab. " Results From January 1, 2004, through June 30, 2019, searches for TED demonstrated a stable baseline level with a mean (SD) relative search volume of 1.78 (1.25). After US Food and Drug Administration approval of teprotumumab in January 2020, TED searches increased by 25%. After the manufacturer of teprotumumab ran its first national television commercial in December 2020, a 525% increase in TED searches was observed. After a second series of national television advertisements for teprotumumab in May 2021, TED searches increased by 640%. Conclusions and Relevance Findings of this cross-sectional study suggest that direct-to-consumer advertising campaigns for teprotumumab may have been associated with an increase in internet search traffic for TED. Google Trends may serve as a useful tool for measuring the influence of pharmaceutical advertising campaigns on information-seeking behavior in the public. Additional studies are warranted to determine how these changes in public interest correlate with clinical measures, such as referral patterns.
The additive effects of photobiomodulation and bioactive glasses on enhancing early angiogenesis

Bioactive glasses (BG) have been widely utilized as a biomaterial for bone repair. However, the early angiogenesis of BG may be inadequate, which weakens its osteogenic effects in large-sized bone defects and often leads to the failure of bone regeneration. In this study, we explored the effects of photobiomodulation (PBM) combined with BG on early angiogenesis to solve this bottleneck problem of insufficient early angiogenesis. In vitro, human umbilical vein endothelial cells (HUVECs) were cultured with BG extracts and treated with PBM using 1 J cm−2. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) and tubule formation assay were utilized to detect HUVECs’ proliferation, vascular growth factor genes expression and tubules formation. In vivo, bone defects at the femoral metaphysis in Sprague-Dawley rats were treated with BG particulates and PBM at 120 J cm−2. Hematoxylin–eosin staining was used to observe the inflammatory response, tissue formation and biomaterial absorption of bone defects. Immunohistochemical staining was applied to observe the vascular-like structure formation. The in vitro results showed that PBM combined with BG significantly promoted HUVECs’ proliferation, genes expression and mature tubules formation. On days 2, 4 and 7, the mRNA expression of VEGF in BG + PBM group was 2.70-, 2.59- and 3.05-fold higher than control (P< 0.05), and significantly higher than PBM and BG groups (P< 0.05). On days 4 and 7, the bFGF gene expression in BG + PBM group was 2.42- and 1.82-fold higher than control (P< 0.05), and also higher than PBM and BG groups (P< 0.05). Tube formation assay showed that mature tubules were formed in BG + PBM and PBM groups after 4 h, and the number in BG + PBM group was significantly higher than other groups (P< 0.05). In vivo results further confirmed PBM induced early angiogenesis, with more vascular-like structures observed in BG + PBM and PBM groups 2 week post-surgery. With the optimum PBM fluence and BG concentration, PBM combined with BG exerted additive effects on enhancing early angiogenesis.
The Physics of Pneumatic Tide Gauges

As a part of its programme to monitor sea level around the British Isles, the Institute of Coastal Oceanography and Tides has a requirement for a recording system which may be quickly and easily installed, and which gives results accurate to 0.01 metre. The traditional stilling-well installation, apart from being costly and difficult to install on a temporary basis, has a number of fundamental disadvantages which limit its ability to record true water levels accurately (Lennon , 1971). Furthermore, stilling-wells, may only be installed where a harbour wall, pier or other vertical structure exists, and such structures are seldom located in positions which are representative of open sea conditions. Pneumatic pressure tide gauges have a number of advantages : not only may they be easily installed on vertical structures, but they may also be used on open coast lines, along beaches, or wherever a light connecting tube can be run from an underwater gas outlet to a recorder ashore. By comparison with gauges which rely on submerged electrical transducers connected to a shore-based recorder by conducting cable, the vulnerable underwater parts of pneumatic gauges are both cheap and easily repaired. Before adopting the pneumatic tide gauge principle for routine measurements it was necessary to investigate the physics of such systems, so that sources of error could be eliminated or subject to correction. This report contains details of these investigations; their application to coastal sea level measurements is described in more detail elsewhere (Pugh, 1971). Details of the bubbler-type gauge which uses a continuous flow of air through a connecting tube to an underwater outlet are given in the first section. However, an extension of the bubbler-gauge theory shows that a flow of air is not strictly necessary, and details of a non-bubbling system are given in the second section. This latter type of gauge is similar to gauges which use a partially inflated bag, but it has the advantage that gauge datum is defined to the same accuracy as a bubbler-gauge datum. The third section of this paper shows how these results may be applied to the design of actual pneumatic tide gauge systems.
Patagonian sheepdog: Genomic analyses trace the footprints of extinct UK herding dogs to South America

Most modern dog breeds were developed within the last two hundred years, following strong and recent human selection based predominantly on aesthetics, with few modern breeds constructed solely to maximize their work potential. In many cases, these working breeds represent the last remnants of now lost populations. The Patagonian sheepdog (PGOD), a rare herding breed, is a remarkable example of such a population. Maintained as an isolated population for over 130 years, the PGOD offers a unique opportunity to understand the genetic relationship amongst modern herding breeds, determine key genomic structure of the founder PGOD populations, and investigate how canine genomic data can mirror human migration patterns. We thus analyzed the population structure of 159 PGOD, comparing them with 1514 dogs representing 175 established breeds. Using 150,069 SNPs from a high-density SNP genotyping array, we establish the genomic composition, ancestry, and genetic diversity of the population, complementing genomic data with the PGOD’s migratory history to South America. Our phylogenetic analysis reveals that PGODs are most closely related to modern herding breeds hailing from the United Kingdom. Admixture models illustrate a greater degree of diversity and genetic heterogeneity within the very small PGOD population than in Western European herding breeds, suggesting the PGOD predates the 200-year-old construction of most pure breeds known today. We thus propose that PGODs originated from the foundational herding dogs of the UK, prior to the Victorian explosion of breeds, and that they are the closest link to a now-extinct population of herding dogs from which modern herding breeds descended.
Rapid Intraspecies Evolution of Fitness Effects of Yeast Genes

Abstract Organisms within species have numerous genetic and phenotypic variations. Growing evidences show intraspecies variation of mutant phenotypes may be more complicated than expected. Current studies on intraspecies variations of mutant phenotypes are limited to just a few strains. This study investigated the intraspecies variation of fitness effects of 5,630 gene mutants in ten Saccharomyces cerevisiae strains using CRISPR–Cas9 screening. We found that the variability of fitness effects induced by gene disruptions is very large across different strains. Over 75% of genes affected cell fitness in a strain-specific manner to varying degrees. The strain specificity of the fitness effect of a gene is related to its evolutionary and functional properties. Subsequent analysis revealed that younger genes, especially those newly acquired in S. cerevisiae species, are more likely to be strongly strain-specific. Intriguingly, there seems to exist a ceiling of fitness effect size for strong strain-specific genes, and among them, the newly acquired genes are still evolving and have yet to reach this ceiling. Additionally, for a large proportion of protein complexes, the strain specificity profile is inconsistent among genes encoding the same complex. Taken together, these results offer a genome-wide map of intraspecies variation for fitness effect as a mutant phenotype and provide an updated insight on intraspecies phenotypic evolution.
Long‐term outcome of dogs recovering from acute kidney injury: 132 cases

Abstract Background Information regarding long‐term outcome of dogs recovering from acute kidney injury (AKI) is limited. Objectives Determine the long‐term outcome of dogs recovering from AKI and identify predictors for serum creatinine concentration (sCr) normalization and long‐term outcome. Animals One hundred thirty‐two dogs with AKI that survived ≥30 days postdischarge. Methods Retrospective study. Search of medical records of dogs diagnosed with AKI that survived to discharge. Follow‐up data were retrieved from medical records and by telephone interviews with the owners or primary care veterinarians or both. Results Estimated median survival time (MST) was 1322 days (95% confidence interval [CI], 1147‐1626), and 76% of the dogs were alive at last contact. Normalization of sCr was documented in 55% of the dogs at discharge and in additional 20% during the follow‐up period. The proportion of dogs with sCr normalization decreased with increase in AKI grade (P = .02). Long‐term survival was not associated with sCr normalization (P = .63). Etiology was associated with the long‐term outcome (P = .004). Conclusion and Clinical Importance Long‐term survival of dogs with AKI is longer than previously described. Normalization of sCr in 99 dogs (75%) occurred, either at discharge or within the follow‐up period. Normalization of sCr was not associated with long‐term survival. Estimated MST of dogs with sCr normalization was not different compared with dogs that developed azotemic chronic kidney disease (CKD), presumably because of slow CKD progression rate. Etiology is an important factor determining sCr normalization and long‐term survival, emphasizing the importance of the reversibility of renal injury rather than its severity.
When do we need to suspect maturity onset diabetes of the young in patients with type 2 diabetes mellitus?

Objetivo: Maturity onset diabetes of the young (MODY) patients have clinical heterogeneity as shown by many studies. Thus, often it is misdiagnosed to type 1 or type 2 diabetes(T2DM). The aim of this study is to evaluate MODY mutations in adult T2DM patients suspicious in terms of MODY, and to show clinical and laboratory differences between these two situations. Subjects and methods: In this study, we analyzed 72 type 2 diabetic patients and their relatives (35F/37M) who had been suspected for MODY and referred to genetic department for mutation analysis. The gene mutations for MODY have been assessed in the laboratory of Marmara University genetics. Totally 67 (32F/35M; median age 36.1) diabetic patients were analyzed for 7 MODY mutations. Twelve patients who have uncertain mutation (VUS) were excluded from study for further evaluation. MODY(+) (n:30) patients and T2DM patients (n:25) were compared for clinical and laboratory parameters. Results: In MODY(+) subjects, mutations in GCK (MODY 2) (n:12; 40%) were the most common followed by HNF4A (MODY 1) (n:4; 13.3%). Diabetes diagnosis age was younger in MODY(+) group but not statistically significant. Sixty-six percent of MODY(+) subjects had diabetes history at 3-consecutive generations in their family compared with 28% of T2DM patients statistically significant (p:0.006). Gender, BMI, C-peptide, HbA1c, lipid parameters, creatinine, GFR, microalbuminuria, vitamin D and calcium were not statistically different between the groups. Conclusion: According to present study results, MODY mutation positivity is most probable in young autoantibody (-) diabetic patients diagnosed before 30 years of age, who have first degree family history of diabetes.
Porphyromonas gingivalis amount in the tongue biofilm is associated with erosive arthritis in systemic lupus erythematosus

Background Several data have demonstrated the occurrence of erosive arthritis in Systemic Lupus Erythematosus (SLE) patients. However, a few studies have focused on the pathogenic mechanisms involved in this feature. The implication of oral pathogens has been proved in Rheumatoid Arthritis: in particular, Porphyromonas gingivalis (Pg), by inducing citrullination, could trigger autoimmune response. Here, we evaluated amount of Pg on the tongue in a cohort of SLE patients with arthritis, focusing on the association with the erosive phenotype. Methods SLE patients with arthritis were enrolled. DAS28 was applied to assess activity. Erosive damage was evaluated by ultrasound at level of MCP (metacarpophalangeal) and PIP (proximal interphalangeals) joints. All subjects underwent a tongue cytologic swab in order to quantify the amount of Pg (real-time PCR). The bacterium expression was obtained from the ratio between the patient’s DNA amount and that obtained from healthy subjects. Results 33 patients were enrolled (M/F 3/30; median age 47 years, IQR 17; median disease duration 216 months, IQR 180): 12 of them (36.4%) showed erosive damage, significantly associated with ACPA positivity (p = 0.03) and higher values of DAS28 (p = 0.01). A mean ratio of 19.7 ± 31.1 was found for Pg amount. Therefore, we used Pg mean values as threshold, identifying two groups of patients, namely, high Pg and low Pg. Erosive damage was significantly more frequent in high Pg patients in comparison with low Pg (60.0% vs 26.0%, p = 0.001). Furthermore, high Pg patients showed higher prevalence of skin manifestations, serositis, and neurological involvement (p = 0.005, p = 0.03, p = 0.0001, respectively). Conclusion The possible contribution of oral microbiota in SLE erosive arthritis was here evaluated for the first time, finding a significant association between erosive damage and higher expression of Pg at tongue level.