Miracle-dz/UniFAR-FTUs huggingface dataset:
Sources and sinks of CO2 and CH4 in siliciclastic subterranean estuaries

Anthropogenic production of greenhouse gases (GHGs) has intensified the need to constrain estimates of natural atmospheric sources from both terrestrial and marine systems. Estuaries are known sources of carbon dioxide (CO2) and methane (CH4); however, less is known about GHG dynamics in subterranean estuaries (STEs). We evaluate CO2 and CH4 dynamics in three proximal STEs bordering Indian River Lagoon, Florida, where groundwater flows through siliciclastic sediments with minor carbonate mineral contents. Although the three STEs have similar mineralogical and flow characteristics, CO2 and CH4 concentrations vary by orders of magnitude. Nonconservative mixing of both gases is observed, and CH4 is generally produced while CO2 is sequestered. The extent of methanogenesis is linked to the redox potential of inflowing groundwaters, as well as degree of CH4 oxidation, which results mostly from anaerobic oxidation of methane. Methane concentrations vary by orders of magnitude, and stable isotopic signatures suggest differences in the microbial production pathway between sites. CO2 is sequestered due to the production of alkalinity relative to dissolved inorganic carbon, which occurs both through rapid CaCO3 dissolution at the shoreline as low‐pH groundwater from the siliciclastic aquifer interacts with carbonate minerals in lagoon sediments, as well as redox reactions, particularly sulfate reduction and denitrification. These results demonstrate a high variability in CO2 and CH4 concentrations, and thus fluxes, even among geographically constrained and hydrogeologically similar STEs. Although STEs are sources of both CO2 and CH4 to surface waters, the variability of production and consumption complicates global estimates of GHG fluxes from STEs.
THE ROLE OF HR PRACTICES ON TURNOVER INTENTIONS DIRECTLY AND THROUGH ORGANIZATIONAL CITIZENSHIP BEHAVIOR IN THE BANKING SECTOR OF MALAYSIA

In the recent years, the banking industry of Malaysia has grown rapidly and the roles of employees are undeniable. In todays, dynamic environment banking industry is striving hard to retain the employees in the organization. Employee provides the basis for organization to be successful. The aim of this paper was to investigate the role of HR practices on turnover intentions directly and indirectly through organizational citizenship behavior in banking sector of Malaysia. Data collected through 510 questionnaires from top five banks (by Capital) branches in Kuala Lumpur, Malaysia. In order to investigate the hypotheses, this study applied Structural equation modelling (AMOS). Findings of this study portray that HR practices had negative effect on turnover intentions while the interesting finding is that indirect effect of HR practices through organizational citizenship were also significant. The results are beneficial for top management of banking sector of Malaysia in order to keep motivating employees and to retain them through proper implementation of OCB. To build and align the HR practices and OCB which results in reducing turnover intentions of employees.
Controlling or Channeling Demands? How Schedule Control Influences the Link Between Job Pressure and the Work-Family Interface

Schedule control is theorized as a job resource that should reduce the extent to which work demands bleed into nonwork time and decrease work-to-family conflict. However, schedule control might also come with greater expectations that workers fully devote themselves to work even during non-conventional work times; in this scenario, schedule control might act as a channel through which job demands can more easily permeate nonwork roles and generate conflict. Drawing on four waves of panel data from the Canadian Work, Stress, and Health Study (2011–2017), the authors use fixed effects regression techniques to discover some contradictions in the resource functions of schedule control. The authors find that schedule control exacerbates the effect of job pressure on role blurring, and these observed downsides of schedule control are stronger for women. By discovering gendered effects in the moderating role of schedule control, this study sharpens prevailing knowledge about its functions as a resource and the ways that it might channel stressful work-related demands.
PREPARATION AND COMPARATIVE EVALUATION OF HAEMORRHAGIC SEPTICAEMIA VACCINES USING EOLANE-150 AND EOLANE-170 AS OIL ADJUVANTS FOR CATTLE AND BUFFALO

Haemorrhagic septicaemia (HS) caused by Pasteurella multocida serotype B:2 is an economically important disease of cattle and buffalo, causes heavy economic losses due to sudden death of animals in developing countries like Pakistan. In this country, animals are vaccinated by alum (adjuvant) precipitated vaccine twice a year. Immunity induced through this prophylactic measure lasts for 3-4 months only. Two new HS oil based vaccines were prepared by using two new oil adjuvants such as Eolane-150 and Eolane-170. The ratio of bacterial antigen and oil adjuvants was 1:1 while bacterial dry weight was adjusted to 2 mg/ml. The addition of enrichments and aeration resulted in dense bacterial growth of Pasteurella multocida. Both new vaccines passed sterility, safety and potency tests as per OIE, 2017. Active and passive Mouse Protection Tests were performed to evaluate its potency. Indirect Haem-Agglutination (IHA) test was conducted on serum samples of two rabbits, groups each of which was vaccinated with HS oil based vaccines adjuvanted with Eolane-150 and Eolane-170. IHA indicated that immune response was higher (GMT=32) initially on 45th day to 75th day post vaccination and then declined (GMT=16) in the rabbits vaccinated with HS vaccine adjuvanted with Eolane-150, while protective immune response remained constant (GMT=16) up to ninety days post vaccination in the rabbits vaccinated with HS vaccine adjuvanted with Eolane-170. Vaccines were easy to inject with no side effects, including swelling at the injection site and longer protection as well. That would hopefully motivate the livestock owners and farmers to use this new product to protect their animals against this fatal HS disease.
Risk factors for severity of COVID-19 in chronic dialysis patients from a multicentre French cohort

Abstract Background Coronavirus disease 2019 (COVID-19) is an emerging infectious disease, related to severe acute respiratory syndrome coronavirus 2 infection. Few data are available in patients with end-stage renal disease (ESRD). Methods We conducted an observational cohort study of COVID-19 patients at 11 dialysis centres in two distinct districts of France to examine the epidemiological and clinical characteristics of COVID-19 in this population, and to determine risk factors of disease severity (defined as a composite outcome including intensive care unit admission or death) and mortality. Results Among the 2336 patients enrolled, 5.5% had confirmed COVID-19 diagnosis. Of the 122 patients with a follow-up superior to 28 days, 37% reached the composite outcome and 28% died. Multivariate analysis showed that oxygen therapy on diagnosis and a decrease in lymphocyte count were independent risk factors associated with disease severity and with mortality. Chronic use of angiotensin II receptor blockers (ARBs) (18% of patients) was associated with a protective effect on mortality. Treatment with azithromycin and hydroxychloroquine (AZT/HCQ) (46% of patients) were not associated with the composite outcome and with death in univariate and multivariate analyses. Conclusions COVID-19 is a severe disease with poor prognosis in patients with ESRD. Usual treatment with ARBs seems to be protective of critical evolution and mortality. There is no evidence of clinical benefit with the combination of AZT/HCQ.
Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines

Abstract Hemocyanins are oxygen-transporting glycoproteins in molluscs and arthropods. In this study, we assayed the biomacromolecules from the molluscs Helix lucorum (HlH), Rapana venosa (RvH) and Megatura crenulata (KLH) including their functional units (FUs), for therapy of bladder cancer permanent cells. In vitro studies antitumour activities of these proteins were performed with bladder cancer permanent cell line CAL-29 and the normal urothelial cell line HL 10/29 in comparison to doxorubicin. The obtained results showed that the human tumour CAL-29 cell line is sensitive to the action of the tested hemocyanins and their isoforms. We observed a dose- and time-dependent inhibition of tumour cell growth after incubation with native HlH and two FUs (βc-HlH-a and FU βc-HlH-h); and of particular significance, FU βc-HlH-h showed a surprisingly stronger effect than that the doxorubicin-treated cells. Cells treated with βc-HlH-h, showed both, apoptotic and necrotic cells. In addition, two-dimensional polyacrylamide gel electrophoresis (PAGE) found for differential up-regulation of several proteins after hemocyanin treatment. Eight different down-regulated and two up-regulated proteins were identified, which may be associated with the apoptosis pathway. No inhibition of the normal urothelial cell line HL 10/29 was observed after treatment with HlH and its isoforms. The most effective inhibition of CAL-29 tumour cells was observed after treatment with βc-HlH-h, probably caused by a specific oligosaccharide structure of HlH with methylated hexoses. These results suggest that hemocyanin glycosylation plays an important role in its anticancer activity.
Evaluation of the Inter and Intra-Observer Reliability of the AO Classification of Intertrochanteric Fractures and the Device Choice (DHS, PFNA, and DCS) of Fixations

Background ArbeitsgemeinschaftfürOsteosynthesefragen (AO) classification is the most frequently used tool to classify intertrochanteric fractures. However, there is limited evidence regarding its reliability. Therefore, this study was designed to evaluate inter-observer and intra-observer reliability of the AO-2018 intertrochanteric fracture classification. Method A retrospective study was conducted in Imam Khomeini Hospital Complex, on radiography of patients who came with intertrochanteric fractures from March 21, 2018, to March 19, 2019. Four orthopedic trauma surgeons assessed 96 anteroposterior pelvic radiographs of intertrochanteric fractures and classified using an AO intertrochanteric fracture classification of 2018. The reading and review of radiography were performed in 2 separate occasions in a 1-month interval. The inter-observer and intra-observer reliability was assessed using kappa statistics. Result The level of both mean inter-observer (K =0.322; 95%CI: 0.321–0.323) and intra-observer agreement (K =0.317; 95%CI: 0.314–0.320) in AO intertrochanteric fracture classification subgrouping were not satisfactory. The inter-observer (K =0.61; 95%CI: 0.608–0.611) and intra-observers' (K=0.560; 95%CI: 0.544–0.566) reliability in AO main groupings showed moderate agreement. Conclusion The AO classification does not show adequate and acceptable inter-observer and intra-observer reliability and reproducibility. Therefore, it will be hard to base on the AO classification for treatment protocols.
An investigation on the effect of gender on parasite distribution in anurans

Two species of anurans ( Rana temporaria and Bufo bufo) were sampled from 4 different sites in kubwa metropolis of the FCT, Abuja, Nigeria. A total of 172 anurans were collected and the blood and digestive tracks were examined for the presence of haemoparasites and intestinal parasites using thick and thin blood smears and the microscopic examination of the intestine. Blood parasites recovered were Trypanosoma rotatarium and microfilarial worms while intestinal parasites recovered were Oxysomatium sp, Rhabdia sp, Mesoceolium burti, Nyetotherus cordiformis and Opalina ranarum. The results revealed 84.78% of Rana temporaria were parasitized and 95.34% of Bufo bufo were parasitized. Males of Rana temporaria were more infected (97.72%) than the females (72.91%) while the females (96.07%) of Bufo bufo were more infected than the males (94.28%). The prevalence of heamoparasites was dominated by microfilarial worms with (79.65%) of the two species of anurans being infected while (70.34%) harboured Trypanosoma rotatarium. The prevalence of the intestinal parasites was dominated by Opalina ranarum ( 84.88%), followed by Oxysomatium sp ( 58.72%), Rhabdia sp (52.32%), Mesocoelium burti (48.25%) and Nyetotherus cordiformis (44.18%) From the results, it was observed that they are no significant relationships between the prevalence and composition of parasites found in the anurans with respect to their gender. This work provides an overview on how anurans can be implicated in the spread of diseases and parasites.
Propagule risk in a marine foundation species: Seascape effects on Zostera marina seed predation

Both biotic and abiotic factors can affect whether seagrass meadows establish as large, continuous features or as a mosaic of smaller patches, and these processes act across multiple spatial scales. Of these processes, sexual recruitment is considered vital for the recovery and resilience of seagrass meadows. Because numerous estuarine fauna can impact sexual recruitment by consuming seeds, it is important to understand variation in seed consumption throughout the seascape. We sought to determine how cross‐scale processes affected seed consumption of Zostera marina, a widely distributed seagrass species. We deployed seed predation units (SPU) within patches ranging in size from 0.28 to 28.48 m2, and on unvegetated, sandy bottom. Seed consumption was analysed across patch sizes, at patch centres and edges and within the context of total seagrass cover within buffer zones surrounding SPU locations. Using structural equation modelling, we found that measured seascape parameters explained 34% of the observed variation in seed consumption. Specifically, we found that seagrass presence had the strongest effect on seed predation followed by a negative effect of patch size on seed consumption. Although there was no direct effect of percent seagrass cover (within a 4‐m buffer of the SPU) on seed predation, percent seagrass cover had a negative indirect effect through its positive correlation with patch size. However, we found that once variation due to patch size and areal cover were accounted for, seeds placed at patch interiors actually experienced greater consumption rates. We did not find similar relationships with our potential seed consumers, although our snapshot sampling likely obscured transient effects. We argue that seagrass recruitment could be affected by seed predation, which is influenced by multiple processes, including predator dispersal, behaviour and aggregation. Since seed‐based restoration is becoming more common, and the role of sexual recruitment in meadow maintenance appears to be increasingly important for seagrass resilience, our results demonstrate that for current management strategies to be successful, there is a need to better understand seed predation in seagrass‐dominated seascapes. Synthesis. Factors that influence propagule supply and recruitment success of seagrass operate and interact across multiple spatial scales, which can have implications for management.
Stochastic Gene Expression Influences the Selection of Antibiotic Resistance Mutations

Bacteria can resist antibiotics by expressing enzymes that remove or deactivate drug molecules. Here, we study the effects of gene expression stochasticity on efflux and enzy-matic resistance. We construct an agent-based model that stochastically simulates multiple biochemical processes in the cell and we observe the growth and survival dynamics of the cell population. Resistance-enhancing mutations are introduced by varying parameters that control the enzyme expression or efficacy. We find that stochastic gene expression can cause complex dynamics in terms of survival and extinction for these mutants. Regulatory mutations, which augment the frequency and duration of resistance gene transcription, can provide limited resistance by increasing mean expression. Structural mutations, which modify the enzyme or efflux efficacy, provide most resistance by improving the binding affinity of the resistance protein to the antibiotic; increasing the enzyme’s catalytic rate alone does not contribute to resistance to ciprofloxacin, but may contribute if drug binding isn’t rate limiting. Overall, we identify conditions where regulatory mutations are selected over structural mutations, and vice versa. Our findings show that stochastic gene expression is a key factor underlying efflux and enzymatic resistances and should be taken into consideration in future antibiotic research.
Environmental Values in American Culture

although, the space given to developing arguments and justifications for positions taken is inadequate. There is a repetitive tendency to fall back on ‘realism’ as the reason why certain positions are untenable, although the author seems to be aware of the limits this places on the potential for human development. What he appears to be unaware of is that his reality is culturally and personally defined rather than being a universal truth. CLIVE L. SPASH University of Cambridge Department of Land Economy 19 Silver Street, CB3 9EP, UK.
The Influence of Maternal Nutrition Consumption Level During Breastfeeding on Breast Milk Macronutrient Component and Infant’s Weight

Fine intake of maternal nutrient consumption was important for the health of mother and the baby. There were previous researches explained that the maternal nutrition consumption level during breastfeeding could influence breast milk macronutrient component. Breast milk macronutrient components can support infants growth process. Analyzing the influence of maternal nutrient consumption level during breastfeeding on breast milk macronutrient component and infant weight. The information regarding maternal nutrition consumption level during breastfeeding was obtained by using food recall for 24 hours in two days. Furthermore, infant birth weight was measured by using Z-score value, while macronutrient component of breast milk by using MIRIS tool with 60 breastfeeding mother as the sample. Statistic test used categorical regression (CATREG). Based on 60 samples, it was showed that average carbohydrate consumption level 384.3 ± 53.3 gram, protein consumption level 81.5 ± 11.9 gram, and fat consumption level 85.1 ± 15.5 gram. There carbohydrate components of milk of 4.7 ± 0.6 gr / dl, protein components of breast milk 2.4 ± 2.2 gr / dl, and fat component of breast milk 3.6 ± 1.4 gr / dl. Fat consumption levels negative influenced towards the carbohydrate components of breast milk (B = -0.356, p = 0.001) and the level of protein and fat consumption level took a positive effect on fat components of breast mik (B = 0218 and B = 0.576, p = 0.029 and p = 0.000). Meanwhile, protein and fat components of breast milk were positive effect on infant weight (B = 0.323 and B = 0.253; p = 0.037 and p = 0.045). Fine fat consumption could produce a good fat componen of breast milk. In addition, protein and fat component of breast milk give impact on infant weight. Keywords: Maternal nutrition, Consumption level, Breastmilk, Macronutrient, Infant’s weight
Distance Education Students’ Cognitive Flexibility Levels and Distance Education Motivations

This study was conducted to examine the relationship between distance education students’ cognitive flexibility levels and their distance education motivations. In the study, cognitive flexibility levels and distance education motivations were also investigated in terms of several variables (gender, age, computer and internet usage time, time spent weekly in distance learning environment and frequency of participation to synchronized classes). The participants of the study consisted of 615 students enrolled in the Turkish Language course which is carried out at a private university in Istanbul. Cognitive Flexibility Scale (Altunkol, 2011) and e-Learning Motivation Survey (Yıldırım, 2012) were employed for data collection. Study results revealed that there is a low level of positive relationship between cognitive flexibility levels of distance education students and their distance education motivations. Time spent in distance learning environment and the frequency of participation to synchronized classes are found as the factors that affect students’ distance education motivation. Computer usage time is found as the only factor that affects students’ cognitive flexibility level. There is not a significant relationship found between gender, age and internet usage time for cognitive flexibility and distance education motivation.
Lipid foulant interactions during the chromatographic purification of virus-like particles from Saccharomyces cerevisiae

The objective of this study was to understand the mechanism of lipid fouling in chromatography through the investigation of a hydrophobic interaction chromatography (HIC) operation. This was motivated by the need to understand this phenomenon during the manufacture of biological products such as vaccines. The systematic approach and novel analytical techniques employed create a unique platform to study fouling of other chromatographic adsorbents and process feed materials. HIC is employed as a primary capture step in the purification of yeast derived hepatitis R surface antigen (HBsAg), where the required cell disruption and detergent liberation steps release high levels of lipid content into the feed stream. From lipid- rich and lipid-depleted feedstocks, comparative analysis was able to quantify the deterioration in HIC performance (binding capacities, purities and recoveries) under successive cycles. Furthermore, a full mass balance on host lipids identified the highly hydrophobic triacylglyceride as the main foulant. Intra-particle distribution and progression of lipid fouling and its effects on material adsorption and diffusion were then examined under confocal laser scanning microscopy (CLSM). In addition, high- resolution scanning electron microscopy (SEM) images of the fouled bead (after 40 cycles) confirmed that a thick lipid layer was building up on the outer bead surface. Based on these findings, the mechanism of fouling was thought to be the rapid accumulation of lipid foulant at the rim of the bead, which was aggravated by the possible diffusion hindrance resulting from multi layer adsorption. Finally, pretreatments to reduce this mechanism of chromatography fouling were evaluated in terms of improvement on feed quality and HIC performance. Selective adsorbent polystyrene XAD-4 demonstrated promising lipid removal capabilities with satisfactory HBsAg VLP recoveries. The improved feed into the column resulted in a three-fold increase in product capacity, whilst the overall yield remained constant over 40 cycles.