Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of Sex Hormone Receptors in Canine Osteosarcoma

Simple Summary Osteosarcoma is a bone tumor that arises from normal bone cells that become malignant. This is a disease that is relatively common in dogs and also affects humans. Dogs are a useful model for human osteosarcoma as they share many similarities including genetic changes, manner of spread and how they respond to treatment. In dogs, the tumors are frequently seen in large breeds and may be caused by environmental and genetic factors. Sex steroids have been shown to regulate bone metabolism directly and indirectly through receptors on bone. The aim of this study was to investigate the expression levels of sex hormone receptors in canine osteosarcoma tissue and cell lines. Our results demonstrate varying levels of receptors in tissue and a high expression of progesterone receptors in the canine osteosarcoma cell lines. Low oxygen levels further increased progesterone receptor expression. Lastly, estradiol decreased the expression of progesterone receptor in one of the cell lines, and progesterone decreased cell proliferation in the same cell line. Further investigation is recommended to determine whether or not sex steroid receptor levels and sensitivity to hormones are related. Abstract Sex steroids regulate bone metabolism directly and indirectly through receptors on bone. Estrogen receptors (ER-∝, ER-β), progesterone receptor (PR), and androgen receptor (AR), have been previously identified on human osteosarcoma (OSA) cells, and are considered to influence tumor growth, but their expression and role in canine OSA is unknown. The aim of this study was to characterize sex hormone receptor expression levels in naturally occurring OSA tissue and in three canine OSA cell lines. The expression of ER-α, ER-β, PR, and AR was investigated using RT-PCR. PR expression levels were also quantified in OSA cells cultured under hypoxic conditions or in the presence of estradiol. The effects of progesterone on cell proliferation were quantified. Results demonstrated varying expression levels of these receptors in five OSA subtypes. OSA cell lines demonstrated high gene expression levels of PR and low gene expression levels of ER-α and ER-β and no gene expression of AR. PR expression was increased in OSA cells cultured under hypoxic conditions in a HIF-∝ independent manner. Interestingly, one cell line expressed very high levels of PR, expression of which decreased in response to estradiol. In addition, progesterone decreased OSA cell proliferation in this particular cell line. Further investigation of the role of sex steroids, particularly PR and its ligands, in regulation of canine OSA is recommended.
On Fast-Decodable Space-Time Block Codes

In this paper, we focus on full-rate, fast-decodable space-time block codes (STBCs) for 2 x 2 and 4 x 2 multiple-input multiple-output (MIMO) transmission. We first derive conditions for reduced-complexity maximum-likelihood decoding, and apply them to a unified analysis of two families of 2 x 2 STBCs that were recently proposed. In particular, we describe a reduced- complexity sphere decoding algorithm suitable for QAM signal constellations. Next, we derive a novel reduced-complexity 4 x 2 STBC, and show that it outperforms all previously known codes with certain constellations.
The Balloon-borne Large Aperture Submillimeter Telescope and Its Rebirth as a Polarimeter

of a dissertation at the University of Miami. Dissertation supervised by Professor J. Gundersen. No. of page in text. (136) The Balloon-borne Large Aperture Submillimeter Telescope (BLAST) is a 1.8 meter Cassegrain telescope that operates in three bands (250, 350, and 500 μm), each with 30% bandwidth. The detection system is comprised of 280 silicon-nitride micromesh bolometers distributed on three focal plane arrays with 30′′, 42′′, and 60′′ FWHM (full width at half max) beam sizes, respectively. BLAST’s goal is to study the evolutionary history and processes associated with star formation. Earth’s atmosphere is opaque to submillimeter radiation and astronomical observations in this wavelength are best conducted at high altitudes. BLAST is designed to be flown above 99.5% of the atmosphere on a stratospheric balloon. BLAST has made three scientific flights and this thesis covers the last two. The second flight was made in 2006 from McMurdo, Antarctica and studied the evolutionary history and processes associated with star formation. For the third flight, BLAST was reconfigured as a polarimeter (BLAST-Pol) and was also launched from McMurdo in December 2010. BLAST-Pol’s objective is to determine what role, if any, magnetic fields play in star formation. This thesis will describe the BLAST-Pol instrument and provide a summery of key observations made by the 2006 flight. For Sharon and Elizabeth
Predictors of Dental Fear and Anxiety in 9-12-year-old Children in Bosnia and Herzegovina

Objectives Oral health in children is additionally burdened with the presence of dental fear and anxiety (DFA). These clinical psychologic entities in their progressive stages inevitably lead to avoidance of dental appointments, which makes prevention and therapy of oral diseases more difficult. Upon the onset of DFA in general, as one of the emotional outcomes of stress in a dental office, most children patients could define the specific stressors that were most intense for them, which could predict the presence of DFA. Aim To examine the predictors of DFA presence in 9-12-year-old children, and investigate how they could explain the DFA occurrence in study participants. Material and Methods The sample consisted of 200 children aged from 9 to 12 years. The DFA presence was determined by the modified version of the CFSS-DS scale. The child's behavior in the dental office during the treatment was evaluated by the trained observer using Venham anxiety and behavior rating scales. Socioeconomic status, characteristics of dental office visits, and previous caries experience were also analyzed. Results The main DFA predictors were related to invasive dental treatments, where the behavior during dental treatment was the most accurate expression of the DFA appearance. Conclusions Invasive dental procedures are the main stressful factor for DFA occurrence. Predisposing factors could strengthen the DFA occurrence.
Characteristics of the Gut Microbiota in Japanese Patients with Premenstrual Syndrome

Purpose The present study aimed to characterize the gut microbiota of individuals with premenstrual syndrome. Patients and Methods The gut microbiota of 24 Japanese women with PMS (PMS group) and 144 healthy Japanese women (control group) were compared. Analysis of the α- and β-diversities and the gut microbial composition at the genus level were performed using 16S rRNA gene sequence data obtained from stool samples. Results A significant difference in age was observed between the PMS and control groups; however, no significant difference was observed in BMI. The α-diversity measured using the Simpson index was significantly higher in the PMS group than the control group. Visualization of the β-diversity using non-metric multidimensional scaling and permutational multivariate analysis of variance (PERMANOVA) showed that the distance of the gut microbiota between the PMS and control groups is significantly different. Furthermore, a significant difference in the composition of the gut microbiota was observed between the PMS and control groups. At the genus level, the abundances of Collinsella, Bifidobacterium, and Blautia were significantly higher in the PMS group than in the control group. In particular, the abundance of Collinsella in the PMS group was approximately 4.5 times higher than that in the control group. To rule out the confounding effect of age in the abundances of Bifidobacterium, Blautia, and Collinsella, the gut microbiota of the PMS and control groups were compared by age group. Results showed that Collinsella had the highest effect size in participants of 30–40 years of age (mean age: 36.39 ± 4.68 years). Conclusion These results suggest that the PMS group possesses a characteristic gut microbiota. In particular, Collinsella was strongly associated with PMS. Since Collinsella has been reported to be associated with diet, dietary interventions such as prebiotics targeting Collinsella may be effective in preventing, improving, and alleviating PMS.
An Exploratory Study of Digital Inequities and Work in the Redevelopment of a Southeastern American City

Nearly 6 million workers support the multi-billion-dollar digital economy as one of the fastest-growing sectors in the U.S. labor market. Middle-skill jobs in the digital economy sector that pay higher wages and do not require a bachelor’s degree are underrepresented people of intersectional marginalized identities. This exploratory study builds upon previous research on digital inequities by examining the digital economy values, behavior, and interests of a small sample of residents in an area of Charlotte, North Carolina with known digital, social, and economic inequities. Analyses included descriptive and bivariate statistics. Given the exploratory nature of the study, no causal inferences are made, however, preliminary findings suggest a need for further research on digital skills training that addresses the intersectional barriers experienced in marginalized communities, and the need for place-based interventions that leverage localized policies in the areas of affordable housing, workforce development, and economic development. Implications and limitations are discussed.
Effects of Compound Mycotoxin Detoxifier on Alleviating Aflatoxin B1-Induced Inflammatory Responses in Intestine, Liver and Kidney of Broilers

In order to alleviate the toxic effects of aflatoxins B1 (AFB1) on inflammatory responses in the intestine, liver, and kidney of broilers, the aflatoxin B1-degrading enzyme, montmorillonite, and compound probiotics were selected and combined to make a triple-action compound mycotoxin detoxifier (CMD). The feeding experiment was divided into two stages. In the early feeding stage (1–21 day), a total of 200 one-day-old Ross broilers were randomly divided into four groups; in the later feeding stage (22–42 day), 160 broilers aged at 22 days were assigned to four groups: Group A: basal diet (4.31 μg/kg AFB1); Group B: basal diet with 40 μg/kg AFB1; Group C: Group A plus 1.5 g/kg CMD; Group D: Group B plus 1.5 g/kg CMD. After the feeding experiment, the intestine, liver, and kidney tissues of the broilers were selected to investigate the molecular mechanism for CMD to alleviate the tissue damages. Analyses of mRNA abundances and western blotting (WB) of inflammatory factors, as well as immunohistochemical (IHC) staining of intestine, liver, and kidney tissues showed that AFB1 aggravated the inflammatory responses through NF-κB and TN-α signaling pathways via TLR pattern receptors, while the addition of CMD significantly inhibited the inflammatory responses. Phylogenetic investigation showed that AFB1 significantly increased interleukin-1 receptor-associated kinase (IRAK-1) and mitogen-activated protein kinase (MAPK) activities (p < 0.05), which were restored to normal levels by CMD addition, indicating that CMD could alleviate cell inflammatory damages induced by AFB1.
Classical Cepheids, what else?

Abstract We present new and independent estimates of the distances to the Magellanic Clouds (MCs) using near-infrared (NIR) and optical–NIR period–Wesenheit (PW) relations. The slopes of the PW relations are, within the dispersion, linear over the entire period range and independent of metal content. The absolute zero points were fixed using Galactic Cepheids with distances based on the infrared surface-brightness method. The true distance modulus we found for the Large Magellanic Cloud—(m − M)0 = 18.48 ± 0.01 ± 0.10 mag—and the Small Magellanic Cloud—(m − M)0 = 18.94 ± 0.01 ± 0.10 mag—agree quite well with similar distance determinations based on robust distance indicators. We also briefly discuss the evolutionary and pulsation properties of MC Cepheids.
First-principles homogenization theory for periodic metamaterials

We derive from first principles an accurate homogenized description of periodic metamaterials made of magnetodielectric inclusions, highlighting and overcoming relevant limitations of standard homogenization methods. We obtain closed-form expressions for the effective constitutive parameters, pointing out the relevance of inherent spatial dispersion effects, present even in the long-wavelength limit. Our results clarify the limitations of quasi-static homogenization models, restore the physical meaning of homogenized metamaterial parameters and outline the reasons behind magnetoelectric coupling effects that may arise also in the case of center-symmetric inclusions.
Nonrelativistic Nambu-Goldstone modes propagating along a Skyrmion line

We study Nambu-Goldstone (NG) modes or gapless modes propagating along a skyrmion (lump) line in a relativistic and non-relativistic O(3) sigma model, the latter of which describes isotropic Heisenberg ferromagnets. We show for the non-relativistic case that there appear two coupled gapless modes with a quadratic dispersion. In addition to the well-known Kelvin mode consisting of two translational zero modes transverse to the skyrmion line, we show that the other consists of a magnon and dilaton, that is, a NG mode for a spontaneously broken internal U(1) symmetry and a quasi-NG mode for a spontaneously broken scale symmetry of the equation of motion. We find that the commutation relations of Noether charges admit a central extension between the dilatation and phase rotation, in addition to the one between two translations found recently. The counting rule is consistent with the Nielsen-Chadha and Watanabe-Brauner relations only when we take into account quasi-NG modes.
Protect + prevent = preserve? Exploring students’ arguments for and attitudes toward conservation

Abstract Nature conservation and environmental protection are not only visible in the media, but also play a role in the everyday life of young people, as evidenced by ‘Fridays for Future’, a demonstration for climate justice. To facilitate the embedding of conservation in school contexts, this study addresses the ideas and approaches which students have with respect to nature conservation, which pro-environmental behaviors they describe, and which variables described in the relevant literature influence their responses. The results of a writing task showed that German students, aged 11–13 years, primarily associate animals with nature conservation and recognize the need for nature conservation. Behavioral intentions primarily demonstrate that students have an existential access to conservation, and only 12% of students implement active behavior in everyday life according to their own statements. Behavioral intentions focus mainly on protection and prevention, which underscores the need to anchor conservation in schools and thereby to increase students’ commitment to preserve nature and the environment.
Assessment of Dengue and Chikungunya Infections among Febrile Patients Visiting Four Healthcare Centres in Yaoundé and Dizangué, Cameroon

Dengue and chikungunya are now widely distributed in Cameroon, but there is still not enough information on their prevalence in different epidemiological settings. This study was undertaken to assess the prevalence of dengue and chikungunya in both urban and rural settings in Cameroon using three diagnostic tools. From December 2019 to September 2021, willing febrile (temperature >38 °C) outpatients visiting four healthcare facilities in the cities of Yaoundé and Dizangué were screened for dengue, and chikungunya. Clinical features of patient were recorded in a form, and their blood samples were analysed using real-time reverse transcriptase-polymerase chain reaction (rtRT-PCR), rapid diagnostic tests (RDTs) and enzyme-linked immuno-sorbent assays (ELISA). Odds ratios were used to determine the level of association between socio-demographic factors, clinical features, and infection status. The Kappa coefficient permitted to assess the level of agreement between RDTs and ELISA. Overall, 301 febrile patients were recruited in the study: 198 in Yaoundé and 103 in Dizangué. The prevalence of infection varied with the diagnostic tool used. For dengue diagnostics, 110 patients were positive to rtRT-PCR: 90 (45.45%) in Yaoundé, and 20 (19.42%) in Dizangué. The prevalence of dengue IgM using ELISA varied from 22.3% in Dizangué to 30.8% in Yaoundé. Dengue IgM rate using RDTs was 7.6% in Yaoundé and 3.9% in Dizangué. For chikungunya, one (0.5%) patient (Yaoundé, suburb) was positive to rtRT-PCR. The prevalence of chikungunya IgM according to ELISA varied from 18.4% in Dizangué to 21.7% in Yaoundé, while it was 4.5% in Yaoundé and 12.6% in Dizangué with RDTs. Only abdominal and retro-orbital pains were significantly associated with acute dengue infection. All four dengue serotypes were recorded, with a predominance of DENV-3 (35.45%) and DENV-4 (25.45%). Rapid Diagnostic Tests for either chikungunya or dengue displayed very poor sensitivity. This study further confirms the high endemicity of both dengue and chikungunya in Yaoundé and Dizangué. These data stress the need for active surveillance and the implementation of vector control measures to prevent the occurrence of outbreaks across the country.
Spatio-temporal variations in hydrochemistry and quality of surface water in Bharathapuzha River Basin, Kerala, India

ABSTRACT The Bharathapuzha river (Length = 209 km; Area = 6186 km2; order (N) = 8th) is the second-largest river in Kerala State, India. The midland part of the river (Area = 1854 km2) has been studied to bring out the variation in hydrochemistry and quality due to anthropogenic and geological influences. The study area is the ‘rice-bowl’ of the State and is characterized by its unique geography, sub-humid to semi-arid climate, industrial activities, and intense agricultural practices. The major ion chemistry of the Bharathapuzha river, based on surface water from 27 locations for three seasons, is mainly controlled by carbonate weathering, with Ca2+ and HCO3 − ions dominating the composition. Gibbs plots revealed rock–water interaction as the major mechanism controlling the surface water chemistry, and scatter diagrams revealed carbonate weathering significantly and silicate weathering marginally contributing the ions to the surface water. Majority of the water quality parameters exhibited values within the desirable limits of WHO guidelines and Bureau of Indian Standards for drinking purposes, and showed distinct seasonal variations. Quality degradation was observed at some locations, where the tributaries were close to industrial units and domestic dwellings. Strong correlation results of K+ with Cl− and PO4 3-, and the presence of organochlorine pesticides aldrin and heptachlor indicate the use of nutrients and pesticides in the study area. The quality assessment revealed that 44% of samples in monsoon, 67% in post-monsoon, and 33% in pre-monsoon were suitable for drinking and domestic purposes, and almost all samples except one were found to be suitable for irrigational purpose.
Effect of Algorithmic Music Listening on Cardiac Autonomic Nervous System Activity: An Exploratory, Randomized Crossover Study

It is proven that music listening can have a therapeutic impact in many clinical fields. However, to assume a curative value, musical stimuli should have a therapeutic logic. This study aimed at assessing short-term effects of algorithmic music on cardiac autonomic nervous system activity. Twenty-two healthy subjects underwent a crossover study including random listening to relaxing and activating algorithmic music. Electrocardiogram (ECG) and non-invasive arterial blood pressure were continuously recorded and were later analyzed to measure Heart Rate (HR) mean, HR variability and baroreflex sensitivity (BRS). Statistical analysis was performed using a general linear model, testing for carryover, period and treatment effects. Relaxing tracks decreased HR and increased root mean square of successive squared differences of normal-to-normal (NN) intervals, proportion of interval differences of successive NN intervals greater than 50 ms, low-frequency (LF) and high-frequency (HF) power and BRS. Activating tracks caused almost no change or an opposite effect in the same variables. The difference between the effects of the two stimuli was statistically significant in all these variables. No difference was found in the standard deviation of normal-to-normal RR intervals, LFpower in normalized units and LFpower/HFpower variables. The study suggests that algorithmic relaxing music increases cardiac vagal modulation and tone. These results open interesting perspectives in various clinical areas.
Exploring a Possible Link between the Fecal Microbiota and the Production Performance of Pigs

Simple Summary The aim of this study is to investigate the relationship between fecal microbiota and the backfat thickness of pigs by 16S rRNA gene sequencing. Our study found that there were significant differences in the composition of microbiota at the species level, characterized by a higher abundance of Lactobacillus reuteri (L. reuteri) in pigs with low-backfat thickness. Additionally, functional profiling of microbial communities indicated that the isoquinoline alkaloid biosynthesis, arachidonic acid metabolism, and styrene degradation were significantly different between low- and high-backfat thickness groups. Furthermore, feeding L. reuteri reduced the intake-to-gain ratio and had the potential to reduce backfat thickness. These findings show that microbiota could alter the production performance of pigs. Abstract The backfat thickness of pigs not only affects the physical properties and taste of meat, but it also closely relates to the reproduction performance of sows. Accumulating evidence indicates that, apart from genetic factors, gut microbiota can also modulate the fat deposition and muscle growth. However, the differential microbiota in pigs with different backfat thickness, and whether microbiota affects backfat thickness, remains elusive. Firstly, 16S ribosomal RNA (16S rRNA) gene sequencing was performed on 62 fecal samples from pigs with different backfat thicknesses, and the compositions of microbiota among different groups with different backfat thicknesses were different. The abundance of Lactobacillus. reuteri (L. reuteri) and Prevotella sp RS2 was significantly higher in pigs with low-backfat thickness than that in pigs with middle and high-backfat thickness; meanwhile, the abundance of Desulfovibrio piger was significantly lower (p < 0.05) in pigs with low-backfat thickness. Furthermore, the functional profiling of microbial communities suggested that the abundance of isoquinoline alkaloid biosynthesis and styrene degradation were significantly lower (p < 0.05) in the low-backfat thickness group than that in middle and high-backfat thickness groups. Finally, L. reuteri fed to Meishan piglets was capable of improving the production performance and had the potential to reduce backfat thickness. This study provides new evidence that microbiota can regulate the phenotype of the host, and dietary supplementation with L. reuteri can improve the production performance of piglets.