Miracle-dz/UniFAR-FTUs huggingface dataset:
The lipid composition of Patagonian toothfish from the Macquarie Island region : ecological and dietary implications within a regional food web

Recent commercial interest in the Patagonian toothfish (Dissostichus eleginoides) in the Macquarie Island region has heightened the need for a better understanding of the life history of this species. Of particular interest are dietary considerations. However, despite the importance of toothfish in the deep-sea food web of this and other Southern Ocean regions, relatively little is known of their diet. Current knowledge is based upon the examination of stomach contents, a technique that fails to identify with any certainty long-term dietary composition. The determination of lipid composition may provide a simple and effective means of determining the diet of toothfish, one that complements traditional techniques. Specifically, many higher order species are thought to deposit fatty acids derived from prey into storage with little or no modification. Constituent fatty acids of a predator may therefore represent a temporal integration of diet. The lipid composition of toothfish was investigated in relation to both physical (sex, age/size) and fishing parameters (region, season, depth, time of day). Each parameter was compared using a variety of statistical methods including multidimensional scaling and linear discriminant analysis. Significant variations in total lipid content, lipid class composition and especially fatty acid composition between fish of different sizes (ages) points to a potential shift in diet as fish mature. Eicosapentaenoic and docosahexaenoic acids were those fatty acids most responsible for the observed variation with size. The influence of the remaining parameters on lipid composition is less pronounced. To determine whether these variations in lipid composition were due to possible variations in diet, a comparison with potential prey species was undertaken. This involved the examination of 21 species of midwater fish, including 8 species of Myctophidae, and 6 species of squid. Many of these species are know to contribute to the diet of toothfish in the Macquarie Island region. The results of statistical analysis suggest that the diet of Patagonian toothfish at Macquarie Island may vary to a far greater extent, relative to size increases, than is suggested by stomach content analysis. For instance smaller sized toothfish are more closely linked to squid, based on fatty acid composition, than are larger toothfish. Most notable with regards to potential fish prey is that the fatty acid compositions of Myctophidae are more closely related to larger rather than smaller toothfish, possibly indicating a shift in dietary preference as toothfish mature.
Legionella pneumophila Proliferation Is Not Dependent on Intracellular Replication

The complexities of the relationship between Legionella pneumophila and other microorganisms for the growth and survival of legionellae in the environment is still not fully understood, though it is generally believed that amoebae play an important role in the natural environment. Although strains of varying virulence are isolated from environmental sources, it is still generally accepted that intracellular replication is important for the ability of L. pneumophila to proliferate in the natural environment. The model water system allowed the development of reproducible biofilms. A diverse but fairly constant consortium of aquatic microorganisms including fungi, bacteria, and protozoa could be maintained in this system. To investigate if legionellae could have the potential to replicate without multiplication within a protozoal host, an avirulent strain of L. pneumophila serogroup 1 Pontiac (Corby Strain) (CAC) was added to the system. If intracellular multiplication is essential for the proliferation of L. pneumophila in aquatic systems, then elimination of trophozoites in the model system would prevent any further growth of legionellae, and consequently numbers would decrease due to dilution by the continuous culture medium.
Pursuing Equity to Combat Fragmentation in the Home Care Sector: A Study of Using Integrated Care Programs to Deliver Health and Social Care to Elderly Persons Living at Home

Integrated care programs (ICPs) deliver care that is coordinated across carers, care sites, and support systems; continuous over time and between visits; tailored to clients' expressed needs and preferences; and based on shared responsibility for optimizing health among clients, carers, and the state. This research asks how ICPs combat issues of fragmentation in a home care sector fundamentally reshaped by neoliberalism. Using a post-positivist epistemological approach, I collect and analyze data from government documents, NGO reports, scholarly literature, and 117 interviews with program administrators, paid and unpaid carers, and elderly clients in five Canadian ICPs working in the home care sector. These include Aging in Place in Ottawa, Ontario; SMILE in South Eastern Ontario; Carefirst in Scarborough, Ontario; CHOICE in Edmonton, Alberta; and Hope Home Health in Hope, British Columbia. My central argument is that ICPs are most useful as a policy solution to fragmented home care when they use policy techniques that promote equitable processes and outcomes as opposed to focusing on enhancing cost-efficiencies for the state. To understand the interrelations among fragmentation, efficiency, equality, and equity, I use a Feminist Political Economy theoretical framework to assess the gendered, classed and racialized impacts of the policy techniques used by ICPs. By looking at which groups are affected through their involvement in ICPs, in what ways, and under what conditions, I find that policy techniques aimed at achieving cost savings for the state often increase inequality/inequity between, and among, clients and carers. Increasing inequality/inequity increases fragmentation. In contrast, ICPs that use policy techniques that challenge neoliberal ways of working often promote equality/equity as their primary policy goal. These techniques help mitigate fragmentation. Understanding if, how and why ICPs meet the expressed needs of clients and carers in different contexts is essential for program administrators looking to improve their programs, as well as for the clients and carers involved in the daily relations of home care. Knowing that ICPs are most useful as a policy solution to fragmented home care when they promote equitable processes and outcomes gives a clear direction for future reforms that can benefit clients and carers alike.
Response of Irish potato (Solanum tuberosum) to the application of potassium at acidic soils of Chencha, Southern Ethiopia.

An experiment was conducted at at acidic soil of Chencha, Southern Ethiopia to investigate the effect of different levels of potassium (K) fertilizer on the tuber yield of potato for two years (2007-2008). The treatments were 0, 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300 kg ha K. Potato varity CIP392618-511 was planted in a plot size of 3.75 x 3.9 m with inter and intra row spacing of 70 cm and 30 cm, respectivelly. The experiment was laid out in RCB design with three replications. The resut revealed that application of K has significantly increased the tuber yield of potato compared with the control in both years. There was a steady increase in the tuber yield of potato for K levels from 30 kg ha up to 150 kg ha in both years. There was also an increase in yield for K levels beyond 150 kg ha, but the increase was with decreasing trend. Combined analysis of the two years data showed that still the highest yield was obtained at K level applied at of 150 kg ha. It increased the total tuber yield from 18 tha in the control to 53 tha (190%). The relationship between K levels and yield is best expressed with quadratic equation of y = -0.000x + 0.0. 301x + 16.0 with a significant R2 = 0.904. The partial budget analysis data showed that the highets net benefit and marigenal rate of return (10755%) was obtained from K applied at 150 kg ha. It is concluded that there is a significant and positive response of potato to applied K and the highest biological and economic yield was obtained at K level of 150 kg ha suggesting that soils of Chencha area are deficient in K. Thus it is recommended that K fertilizer should be imported and applied in acidic soils of Chencha area to mitigate K deficiency for enhanced potato yield. © 2011 Friends Science Publishers Key Word: Potasium (K); Potato; Soil acidity
Post-Neonatal tetanus from broomstick injuries: a word of caution for caregivers

Tetanus is a vaccine-preventable disease that is acquired through exposure to the spores of the bacterium Clostridium tetani which are ubiquitous in the soil. Post-neonatal tetanus is an increasing problem in developing countries, including Nigeria. This is mainly as a result of inadequate immunization coverage, unsustainable immunization programmes, and non-administration of booster doses of tetanus toxoid at appropriate periods to eligible children. The spores of the bacterium can get into the body through broken skin, commonly through injuries from contaminated objects. There have also been reports of broomstick injury as the portal of entry. Indeed, it has been reported to be the commonest portal of entry in a center in Nigeria. This is important because flogging (of children) with brooms is common in parts of Nigeria, despite it being forbidden in several cultures in the country. Two cases of post-neonatal tetanus arising from broomstick injuries in Uyo, Nigeria, are herein reported, to illustrate the inherent dangers and implications of broomstick injuries in children. It is hoped that advocacy campaigns will be conducted at different public health fora to raise awareness of the general population concerning post-neonatal tetanus from broomstick injuries. Furthermore, there is urgent need to institutionalize the administration of booster doses of tetanus toxoid at primary and secondary school entry.
Umbilical cord twist – Does it play a role in placental morphology?

Studies have described the placental morphology and its changes in a pathological scenario. But the role of a twisting pattern of umbilical vessels in determining the placental morphology of uncomplicated pregnancy has not been discussed. The objective of the study was to determine the clinical significance of umbilical cord twist in determining the umbilical cord coiling index, the diameter of hyrtl’s anastomosis, branching pattern of the placental vasculature, placental weight, Eccentricity index and Cord centrality index. The proportion of umbilical cords with left and right twist were 246 (78.6%) and 67 (21.4%) respectively. The right twisted cords had significant higher umbilical artery diameter, higher umbilical cord coiling index and preferential magistral pattern of blood vessels. This proves that twisting of the cord might play a minor role in altering the blood flow and determining the vasculature pattern but not sufficient enough to influence the placental weight, the shape of the placenta and umbilical cord insertion.
Karyotypic Analysis of the Spined Toad, Bufo spinosus Daudin, 1803 (Amphibia: Bufonidae)

For the first time, somatic chromosomes of Bufo spinosus were described (2 n = 22). It was close to the karyotypes of other West-Palearctic species of the genus Bufo and consisted of 10 large metacentric ( V ), 2 large submetacentric chromosomes ( SV ), 8 small metacentric ( v ), and 2 small submetacentric chromosomes ( sv ): 10 V + 2 SV + 8 v + 2 sv , NF = 44. All chromosomes of B. spinosus were characterized by presence of heterochromatin blocks in centromeric regions. No Q-stained bright bands were found. Ag-staining detected a nucleolar organizer region in the end of the long arm of the sixth pair of chromosomes. In all males studied, a dimorphism was found in the sixth pair of chromosomes. One chromosome in this pair was smaller than the second one. Differences in position of nucleolar organizer region or centromeric index between these chromosomes were not revealed.
"Capnocytophaga canimorsus" : discovery of a deglycosylation mechanism that links metabolism to pathogenesis

We show that C. canimorsus (Cc) can serve as a recipient for RP4 mediated conjugation but there is neither replication of broad host range plasmid vectors nor expression of commonly used E. coli markers in C. canimorsus. We identified three selection markers, ermF, tetQ and cfxA leading to resistance against erythromycin, tetracycline and cefoxitin, respectively, that can be used in C. canimorsus. We engineered expression shuttle vectors using the replicon of a endogenous plasmid found in strain Cc7 and the promoter of one of the selection markers for gene expression. We developed a transposon mutagenesis strategy based on Tn4351 from Bacteroides fragilis and protocols for allelic exchange and electrotransformation. We carried out an extensive transposon mutagenesis and screened these mutants for different properties. We demonstrate that presence of mammalian cells, including phagocytes, favors growth of C. canimorsus 5 and this property was found to be dependent on direct cellular contacts. We isolated a Tn mutant unable to grow in presence of mammalian cells. The mutation occurred in a gene encoding a sialidase. The surface-exposed sialidase allows Cc5 to feed on internal aminosugars of glycan chains from host cell glycans. In addition, sialidase confers resistance to complement by promoting the binding of factor H. We developed an experimental mouse infection in which the read-out is bacterial persistence. In this infection model, Cc5, but not the sialidase deficient mutant, grew and persisted, showing the importance of this metabolic pathway in vivo. C. canimorsus by itself does not elicit the onset of an inflammatory response from macrophages. One strain, Cc5 turned out to have a mechanism that actively blocks the pro-inflammatory signaling of macrophages upon stimulation with endotoxic LPS. We screened the Tn mutant library for clones of Cc5 affected in this active mechanism. Isolated mutants have been mapped, characterized and complemented. The function of the mutated genes is presently under investigation as well as the mode of action of its gene product(s). The prevalence of C. canimorsus in dogs has not been clarified at present. We therefore sampled dog swabs to isolate C. canimorsus strains in Swiss dogs. We could identify 61 C. canimorsus isolates from 103 dogs, which represents 59.22% of the dogs tested. Besides this I also contributed to the analysis of LPS, to the study of resistance of Cc5 to complement mediated lysis, to sequencing of the genome, the assembly of the reads and the annotation.
Effect of NPK, vermicompost and spacing on growth and yield of broccoli (Brassica oleracea L. var. italica)

The experiment was conducted during winter season of during 2016-17 and 2017-18 at the Horticulture farm of Udai Pratap, Autonomous College, Varanasi (U.P.), is situated in the South eastern part of Varanasi city was carried out during winter season. The experimental material used for the “Effect of NPK, Vermicompost and Spacing on growth and yield of Broccoli (Brassica oleracea L. var. italica)” The materials used, experimental procedures followed and techniques adopted during the course of experimentation were planted at a spacing of 45cm x 45cm and 45cm x 60cm per plot under open conditions. The experiment was laid out in RBD comprising of Sixteen treatments with three replication study F3 (180:80:80 NPK kg ha) and minimum in F1 (140:40:40 NPK kg ha) and at 45 and 60 DAP the minimum plant height was recorded with F4 (200:100:100 kg ha) and minimum with F1 (200:100:100 kg ha) and minimum with F1 (140:40:40 NPK kg ha) during both the years of study. In case of spacing S1 (45 x 45 cm) recorded maximum and minimum in S2 (45 x 60 cm) at all the stages during both the years of experimentation. (T1)140:40:40 NPK kg/ha + 45x45cm. Spacing + 20kgVermicompost/ha, (T2)-140:40:40 NPK kg/ha + 45x45cm. Spacing + 10 kg PSB/ha, (T3) -140:40:40 NPK kg/ha + 45x60cm. Spacing + 20 kg Vermicompost/ha (T4)-140:40:40 NPK kg/ha + 45x60cm. Spacing + 10kgPSB/ha (T5)160:60:60 NPK kg/ha + 45x45cm. Spacing + 20kgVermicompost/ha, (T6)-160:60:60 NPK kg/ha + 45x45cm. Spacing +10kg PSB/ha, (T7)-160:60:60 NPK kg/ha + 45x60cm. Spacing + 20kgVermicompost/ha, (T8) 160:60:60 NPK kg/ha + 45x60cm. Spacing + 10kg PSB/ha (T9) 180:80:80 NPK kg/ha + 45x45cm. Spacing + 20 kg Vermicompost /ha (T10) V180:80:80 NPK kg/ha + 45x45cm. Spacing + 10kg PSB/ha (T11) 180:80:80 NPK kg/ha + 45x60cm. Spacing +20kgVermicompost/ha (T12) 180:80:80 NPK kg/ha + 45x60cm. Spacing + 10kg PSB/ha (T13) 200:100:100 NPK kg/ha + 45x45cm. Spacing + 20kgVermicompost/ha (T14) 200:100:100 NPK kg/ha + 45x45cm. Spacing + 10kg PSB/ha (T15) 200:100:100 NPK kg/ha + 45x60cm. Spacing + 20kg Vermicopost/ha (T16) 200:100:100 NPK kg /ha + 45x60cm. Spacing+ 10 kg PSB/ha. The highest yield q ha was recorded in treatment F4 (200:100:100 NPK kg ha), while minimum in F1 (140:40:40 NPK kg ha) during 2016-17 and 2017-18. The improvement was observed in yield q ha in S1 (45 x 45 cm.) spacing over S2 (45 x 60 cm.) spacing application of B2 (PSB) recorded maximum which was at par with B1 and significantly superior over B1 (Vermicompost).
Genetical study for quality traits in green sprouting broccoli (Brassica oleracea var italica)

An experiment was conducted during 1999-2000 to generate genetic information on quality traits in green sprouting broccoli (Brassica oleracea L. var italica Plenck). Significant differences for protein and ascorbic acid among 19 diverse genotypes revealed presence of sufficient variability for these traits, which was confirmed by broad range of variability. Little or no difference between genotypic coefficient of variation (gcv) and phenotypic coefficient of variation (pcv) in both the traits indicated that these were less influenced by environment. High heritability (99.20%) coupled with high genetic advance (78.11%) was observed for ascorbic acid, indicating role of additive gene action for its inheritance. However, protein content had moderate heritability (69.60%) and low genetic advance (8.98%). The genotypic correlation indicated strong inherent association among protein and ascorbic acid and ascorbic acid was positively associated with marketable yield/plant. The direct contribution of ascorbic acid was negative but its indirect contribution via protein was positive.
New evidence for facultative male die-off in island populations of dibblers, Parantechinus apicalis

The post-mating mortality of males in dasyurid species is thought to be a specific life-history strategy, associated with a tightly synchronised breeding season, that may enhance survivorship of juveniles. Parantechinus apicalis has been reported to exhibit male die-off in island populations but not in mainland populations, and males originating from both island and mainland populations are known to survive post-mating in captivity. However, males in the island populations have been reported to sometimes survive to breed in a second year. This study monitored captive and island populations over a three-year period (April 1997 – May 2000) to examine the extent of male die-off. In captivity, males showed no evidence of post-mating mortality. Males on one island were also found to survive post-mating, while another island population appeared to have high mortality of males in three years, and a complete male die-off after the 1999 breeding season. We conclude that P. apicalis may warrant inclusion in a new category of life-history strategy for species exhibiting facultative male die-off.
A Study of Pediatric Nurse’s Emotional Labor, Empowerment, Pediatric Nurse-Caregiver Partnership

This study was conducted to understand the relationship between pediatric nurses’ empowerment, Emotional Larbor and partnership with child patients’ parents. In addition, we aimed to provide basic data for improving pediatric nurses' adatation to tasks and their partnership with child patients' parents. Data collection was conducted in 7 institutes, including general hospitals, university hospitals, and the division of pediatrics of the children's hospitals located in D and C cities among 142 pediatric nurses between July Received (April 21, 2017), Review Result (May 9, 2017) Accepted (May 16, 2017), Published (July 31, 2017) 35233 Dept. of Nursing, Eulji University Hospital. , 95, Dunsanseo-ro, Seo-gu, Daejeon, Korea email: juyou915@eulji.ac. kr (Corresponding Author) 34824 College of Nursing, Eulji Univ., 77, Gyeryong-ro 771beon-gil, Jung-gu, Daejeon, Korea email: ahanaya@eulji.ac. kr 3 34824 Dept. of Nursing, Graduate School, Eulji Univ., 77, Gyeryong-ro 771beon-gil, Jung-gu, Daejeon, Korea email: kem5757@naver.com A Study of Pediatric Nurse’s Emotional Labor, Empowerment, Pediatric Nurse-Caregiver Partnership Copyright c 2017 HSST 36
The lamprey provides a vertebrate blueprint of the mammalian basal ganglia

The basal ganglia are a group of subcortical nuclei that play a prominent role in motor function in mammals as well as in lamprey. The aim of the present study was to characterize the different components of the lamprey basal ganglia, and determine to what extent they correspond to those found in the mammalian basal ganglia. Anatomical tract tracing, immunohistochemistry and acute brain slice patch clamp recordings were employed to address this question.Two pallidal regions were identified in the lamprey; one region, considered homologous to the mammalian globus pallidus, was located ventral to the ementia thalami on the telencephalic/diencephalic border. It receives striatal input from inwardly rectifying neurons (IRNs) and contains GABAergic projection neurons, of which those projecting to the tectum were shown to be tonically active. It also contains neurons immunoreactive for parvalbumin. Separate subpopulations of pallidal neurons project to the optic tectum, the diencephalic and mesencephalic locomotor regions (MLR). Another region, in the midbrain, considered homologous to the substantia nigra pars reticulata receives input from a different subset of IRNs and sends GABAergic projections to the tectum and the diencephalic locomotor region. This midbrain region also contains parvalbumin immunoreactive neurons. The main population of striatal neurons, IRNs, displays the anatomical and electrophysiological hallmarks of mammalian medium spiny neurons, including inward rectification and ramping responses to first spike. It also contains neurons with properties similar to fast-spiking neurons. The striatum receives pallial and thalamic input as well as ascending dopaminergic, serotonergic and histaminergic inputs, similar to that in mammals.Our results suggest that the basic features of the basal ganglia with regard to both structure and function are conserved throughout the vertebrate phylogeny, including striatal/pallidal subdivisions.
In vitro multiplication and protocorm development of Dendrobium aphyllum (Roxb.) CEC Fisher

Fifteen weeks old seeds of D. aphyllum (Roxb.) CEC Fisher germinated on the MS media. The seed germination (90.6%) was recorded. The seed showed development of protocorm like bodies (PLBs) within five weeks of inoculation and the aforesaid viability was observed. The PLBs then started germinating in the basal MS media and were subsequently transferred in the media supplemented with auxin & kinetin separately and mixed through. The growths were recorded after two weeks of inoculation into supplemented media. The PLBs showed development of distinct roots and shoots in media supplemented with IAA & KN. The PLBs showed better germination and developed into multiple roots and shoots, when supplemented with IAA and KN separately compared with the mixture of both IAA & KN application. The average length of roots and shoots are measured and found to be IAA (0.38 cm & 1.68 cm), KN (0.43 cm & 0.45 cm) & IAA+KN (0.50 cm & 0.78 cm) respectively.