Absence of 2NS/2AS in wheat resistance sources to Magnaporthe oryzae in Brazil.

5 Resumo . 7 Introduction . 8 Material and Methods . 10 Results and Discussion . 13 Conclusions . 18 Acknowledgments . 18 References . 18 Absence of 2NS/2AS in Wheat Resistance Sources to Magnaporthe oryzae in Brazil Jéssica Rosset Ferreira1 Camila Vancini2 Carolina Cardoso Deuner3 Gisele Abigail Montan Torres4 Luciano Consoli5 Claudine Dinali Santos Seixas6 Alexei de Campos Dianese7 Augusto César Pereira Goulart8 Hebert Hernán Soto-Gonzáles9 Abstract – Searching for resistance sources to Magnaporthe oryzae in wheat has been a major concern regarding wheat blast in Brazil, since its first report, in 1985. The aim of this work was to evaluate the presence of the translocation 2NS/2AS in wheat accessions identified as resistant, under Brazilian field conditions. The presence of this translocation was evaluated using public primers for amplification of one fragment of 262 base pairs in length. The line VPM1 and the cultivar Thatcher were employed as positive and negative controls for PCR reactions. In all, 17 genotypes (two susceptible and 15 resistant to M. oryzae) were characterized for the presence of the 2NS/2AS translocation. Both susceptible cultivars (Anahuac 75 and BRS 209) lack the 2NS/2AS translocation. Among the resistant ones, two (CBFusarium ENT014 and CPAC 07434) have the 2NS/2AS translocation. Among the resistant cultivars that do not have the translocation are BRS 229 and Trigo Searching for resistance sources to Magnaporthe oryzae in wheat has been a major concern regarding wheat blast in Brazil, since its first report, in 1985. The aim of this work was to evaluate the presence of the translocation 2NS/2AS in wheat accessions identified as resistant, under Brazilian field conditions. The presence of this translocation was evaluated using public primers for amplification of one fragment of 262 base pairs in length. The line VPM1 and the cultivar Thatcher were employed as positive and negative controls for PCR reactions. In all, 17 genotypes (two susceptible and 15 resistant to M. oryzae) were characterized for the presence of the 2NS/2AS translocation. Both susceptible cultivars (Anahuac 75 and BRS 209) lack the 2NS/2AS translocation. Among the resistant ones, two (CBFusarium ENT014 and CPAC 07434) have the 2NS/2AS translocation. Among the resistant cultivars that do not have the translocation are BRS 229 and Trigo 1 Biologist, M.Sc. in Crop Science (Plant Breeding and Biotechnology), internship at Embrapa Trigo, Ph.D student from Agronomy Graduate Program, Passo Fundo University, RS, scholarship by CAPES/Embrapa. 2 Biologist, M.Sc. in Agronomy, internship at Embrapa Trigo, Ph.D student from Agronomy Graduate Program, Passo Fundo University, RS, scholarship by CAPES. 3 Agronomist, PhD in Agronomy (Phytopathology), professor at Passo Fundo University, RS. 4 Agronomist Engineer, PhD in Genetics and Molecular Biology, researcher at Embrapa Trigo, Passo Fundo, RS. 5 Agronomist Engineer, PhD in Genetics and Molecular Biology, researcher at Embrapa Trigo, Passo Fundo, RS. 6 Agronomist Engineer, PhD in Phytopathology, researcher at Embrapa Soja, Londrina, PR. 7 Biologist, PhD in Phytopathology, researcher at Embrapa Cerrados, Planaltina, DF. 8 Agronomist Engineer, M.Sc. in Phytopathology, researcher at Embrapa Agropecuária Oeste, Dourados, MS. 9 Biologist, Ph.D in Biotechnology, professor and researcher, Binational Special Project Lago Titicaca PELT, Puno, Peru. 6 BOLETIM DE PESQUISA E DESENVOLVIMENTO 90 BR 18-Terena, which are examples of durable resistance to wheat blast in Brazil. This work offers new perspectives on the exploration of resistance sources to M. oryzae in addition to the 2NS/2AS translocation.