Miracle-dz/UniFAR-FTUs huggingface dataset:
Approach to the specificity and selectivity between D2 and D3 receptors by mutagenesis and binding experiments part I: Expression and characterization of D2 and D3 receptor mutants

D3/D2 sub‐specificity is a complex problem to solve. Indeed, in the absence of easy structural biology of the G‐protein coupled receptors, and despite key progresses in this area, the systematic knowledge of the ligand/receptor relationship is difficult to obtain. Due to these structural biology limitations concerning membrane proteins, we favored the use of directed mutagenesis to document a rational towards the discovery of markedly specific D3 ligands over D2 ligands together with basic binding experiments. Using our methodology of stable expression of receptors in HEK cells, we constructed the gene encoding for 24 mutants and 4 chimeras of either D2 or D3 receptors and expressed them stably. Those cell lines, expressing a single copy of one receptor mutant each, were stably constructed, selected, amplified and the membranes from them were prepared. Binding data at those receptors were obtained using standard binding conditions for D2 and D3 dopamine receptors. We generated 26 new molecules derived from D2 or D3 ligands. Using 8 reference compounds and those 26 molecules, we characterized their binding at those mutants and chimeras, exemplifying an approach to better understand the difference at the molecular level of the D2 and D3 receptors. Although all the individual results are presented and could be used for minute analyses, the present report does not discuss the differences between D2 and D3 data. It simply shows the feasibility of the approach and its potential.
A core musculoskeletal anatomy syllabus for undergraduate physical therapy student education

Knowledge of musculoskeletal anatomy is fundamental to physical therapy education. Although detailed anatomy syllabi have been developed for medical and other health professional students, none are available for training physical therapists. Therefore, the aim of this project was to produce a core musculoskeletal anatomy syllabus specific to physical therapy students, utilizing a modified Delphi approach. An international Delphi panel (n = 53) composed of anatomists and clinicians involved in physical therapy education considered a total of 2193 anatomical items arranged in four categories (musculoskeletal concepts; vertebral column; pectoral girdle and upper limb; pelvic girdle and lower limb). Using specific criteria, items were rated on the basis of whether they were considered essential knowledge for a competent physical therapy student, and were categorized as core, recommended, not recommended or not core. Of the 2193 items, 1700 (77.5%) were rated as core or recommended. For musculoskeletal concepts, 70% (233/332) of items were categorized as core/recommended. Approximately 80% of items in the other three categories were considered core/recommended—vertebral column (355/440), pectoral girdle and upper limb (513/657), and pelvic girdle and lower limb (599/764). This project presents a detailed musculoskeletal anatomy syllabus which may be used within a physical therapy curriculum. A key difference compared to published musculoskeletal anatomy syllabi for medical students is that a greater number of items are considered core/recommended (approximately 80% vs. 50%). These findings will now be considered and deliberated in accordance with the International Federation of Associations of Anatomists modified Delphi approach.
Propriétés asymptotiques des jeux répétés à somme nulle

Dans cette these nous nous interessons aux jeux repetes a somme nulle, dont la structure recursive peut s'exprimer au moyen d'un operateur de Shapley. Nous etudions le comportement asymptotique de la valeur vn (resp. Vλ) du jeu en n etapes (resp. Du jeu λ-escompte) lorsque n tend vers +∞ (resp. Lorsque n tend vers 0). Dans une premiere partie, nous considerons certaines proprietes analytiques verifees par les operateurs de Shapley et leurs iteres. Pour plusieurs categories de jeux cette approche en terme d'operateur etablit la convergence des deux familles de valeurs vers une limite commune. Dans une deuxieme partie, nous etudions certaines equations d'evolution qui sont des analogues en temps continu des formules caracterisant vn et vλ ; on demontre que les solutions de ces equations ont le meme comportement asymptotique que celles des equations en temps discret.
THE NUCLEAR STRUCTURE IN NEARBY LUMINOUS INFRARED GALAXIES: HUBBLE SPACE TELESCOPE NICMOS IMAGING OF THE GOALS SAMPLE

We present results of Hubble Space Telescope (HST) NICMOS H-band imaging of 73 of the most luminous (i.e., log[LIR/L☉]>11.4) infrared galaxies (LIRGs) in the Great Observatories All-sky LIRG Survey. This data set combines multi-wavelength imaging and spectroscopic data from space-based (Spitzer, HST, GALEX, and Chandra) and ground-based telescopes. In this paper, we use high-resolution near-infrared data to recover nuclear structure that is obscured by dust at optical wavelengths and measure the evolution in this structure along the merger sequence. A large fraction of all galaxies in our sample possess double nuclei (∼63%) or show evidence for triple nuclei (∼6%). Half of these double nuclei are not visible in the HST B-band images due to dust obscuration. The majority of interacting LIRGs have remaining merger timescales of 0.3–1.3 Gyr, based on the projected nuclear separations and the mass ratio of nuclei. We find that the bulge luminosity surface density LBulge/R2Bulge increases significantly along the merger sequence (primarily due to a decrease of the bulge radius), while the bulge luminosity shows a small increase toward late merger stages. No significant increase of the bulge Sérsic index is found. LIRGs that show no interaction features have on average a significantly larger bulge luminosity, suggesting that non-merging LIRGs have larger bulge masses than merging LIRGs. This may be related to the flux-limited nature of the sample and the fact that mergers can significantly boost the IR luminosity of otherwise low luminosity galaxies. We find that the projected nuclear separation is significantly smaller for ULIRGs (median value of 1.2 kpc) than for LIRGs (median value of 6.7 kpc), suggesting that the LIRG phase appears earlier in mergers than the ULIRG phase.
Nitrogen starvation induces genome-wide activation of transposable elements in Arabidopsis.

Nitrogen (N) availability is a major limiting factor for plant growth and agricultural productivity. Although the gene regulation network in response to N starvation has been extensively studied, it remains unknown whether N starvation has an impact on the activity of transposable elements (TEs). Here, we report that TEs can be transcriptionally activated in Arabidopsis under N starvation conditions. Through genetic screening of idm1-14 suppressors, we cloned GLU1, which encodes a glutamate synthase that catalyzes the synthesis of glutamate in the primary N assimilation pathway. We found that GLU1 and its functional homologs GLU2 and GLT1 are redundantly required for TE silencing, suggesting that N metabolism can regulate TE activity. Transcriptome and methylome analyses revealed that N starvation results in genome-wide TE activation without inducing obvious alteration of DNA methylation. Genetic analysis indicated that N starvation-induced TE activation is also independent of other well established epigenetic mechanisms, including histone methylation and heterochromatin decondensation. Our results provide new insights into the regulation of TE activity under stressful environments in planta. This article is protected by copyright. All rights reserved.
Artificial Intelligence in Lung Imaging

Abstract Recently, interest and advances in artificial intelligence (AI) including deep learning for medical images have surged. As imaging plays a major role in the assessment of pulmonary diseases, various AI algorithms have been developed for chest imaging. Some of these have been approved by governments and are now commercially available in the marketplace. In the field of chest radiology, there are various tasks and purposes that are suitable for AI: initial evaluation/triage of certain diseases, detection and diagnosis, quantitative assessment of disease severity and monitoring, and prediction for decision support. While AI is a powerful technology that can be applied to medical imaging and is expected to improve our current clinical practice, some obstacles must be addressed for the successful implementation of AI in workflows. Understanding and becoming familiar with the current status and potential clinical applications of AI in chest imaging, as well as remaining challenges, would be essential for radiologists and clinicians in the era of AI. This review introduces the potential clinical applications of AI in chest imaging and also discusses the challenges for the implementation of AI in daily clinical practice and future directions in chest imaging.
Tecovirimat therapy for severe monkeypox infection: Longitudinal assessment of viral titers and clinical response pattern—A first case‐series experience

Tecovirimat is the only antiviral with conditional and emergency approval for treatment of monkeypox by the European Medicines Agency. We present longitudinal virologic data of three patients with severe monkeypox treated with Tecovirimat. These patients presented with underlying comorbidities (ulcerative colitis; acute syphilis; HIV infection) and higher baseline monkeypox viremia compared to patients with less severe disease courses. Tecovirimat was well-tolerated, and clinical improvement was observed. In median viremia decreased by more than 2 logs within one week of therapy. This article is protected by copyright. All rights reserved.
Major elective non-cardiac operations in adults with congenital heart disease

Objective To assess the impact of congenital heart disease (CHD) on resource utilisation and clinical outcomes in patients undergoing major elective non-cardiac operations. Background Due to advances in congenital cardiac management in recent years, more patients with CHD are living into adulthood and are requiring non-cardiac operations. Methods The 2010–2018 Nationwide Readmissions Database was used to identify all adults undergoing major elective operations (pneumonectomy, hepatectomy, hip replacement, pancreatectomy, abdominal aortic aneurysm repair, colectomy, gastrectomy and oesophagectomy). Multivariable regression models were used to categorise key clinical outcomes. Results Of an estimated 4 941 203 adults meeting inclusion criteria, 5234 (0.11%) had a previous diagnosis of CHD. Over the study period, the incidence of CHD increased from 0.06% to 0.17%, p<0.001. CHD patients were on average younger (63.3±14.8 vs 64.4±12.5 years, p=0.004), had a higher Elixhauser Comorbidity Index (3.3±2.2 vs 2.3±1.8, p<0.001) and received operations at high volume centres more frequently (66.6% vs 62.0%, p=0.003). Following risk adjustment, these patients had increased risk of in-hospital mortality (adjusted risk ratio (ARR): 1.76, 95% CI 1.25 to 2.47), experienced longer hospitalisation durations (+1.6 days, 95% CI 1.3 to 2.0) and cost more (+$8370, 95% CI $6686 to $10 055). Furthermore, they were more at risk for in-hospital complications (ARR: 1.24 95% CI 1.17 to 1.31) and endured higher adjusted risk of readmission at 30 days (ARR: 1.32 95% CI 1.13 to 1.54). Conclusions Adults with CHD are more frequently comprising the major elective operative cohort for non-cardiac cases. Due to the inferior clinical and financial outcomes suffered by this population, perioperative risk stratification may benefit from the inclusion of CHD as a factor that portends unfavourable outcomes.
Internet use and psychological wellbeing among older adults in England: a difference-in-differences analysis over the COVID-19 pandemic

Abstract Background Longitudinal evidence on how Internet use affects the psychological wellbeing of older adults has been mixed. As policymakers invest in efforts to reduce the digital divide, it is important to have robust evidence on whether encouraging Internet use among older adults is beneficial, or potentially detrimental, to their wellbeing. Methods We observe depressive symptoms and loneliness of adults aged 50 + in the nationally representative English Longitudinal Study of Ageing, from before (2018/19) to during the coronavirus disease 2019 (COVID-19) pandemic (June/July and November/December 2020). Our quasi-experimental difference-in-differences strategy compares within-individual wellbeing changes between older adults who desired to use the Internet more but experienced barriers including lack of skills, access, and equipment, with regular Internet users who did not desire to use the Internet more. To reduce selection bias, we match both groups on demographic and socioeconomic characteristics that are predictive of Internet use. We assume that in the absence of COVID-19 – a period of increased reliance on the Internet – the wellbeing trajectories of both groups would have followed a common trend. Results Compared with matched controls (N = 2983), participants reporting barriers to Internet use (N = 802) experienced a greater increase in the likelihood of depressive symptoms from before to during the pandemic, but not worse loneliness levels. This effect was stronger for women, those aged above 65 years, and those from lower-income households. Conclusions Besides enabling access to digital services, efforts to ensure older adults continue to be engaged members of an increasingly digital society could deliver returns in terms of a buffer against psychological distress.
Influence of Turfgrass Parameters on the Abundance of Arthropods in Sod Farms

Abstract Sod farms, where turfgrass is commercially produced, have a unique system, in which sod is harvested within 2 yr after planting. Understanding the turfgrass factors that influence the abundance of predators, herbivores, detritivores, and parasitoids that inhabit turfgrass paves the foundation for developing effective pest management programs. However, little is known about those factors in sod farms. The objective of the study was to determine the influence of turfgrass height, density, and thatch thickness on abundance of arthropod taxa in sod farms. The study was conducted at 18 and 10 sod field sites in 2019 and 2020, respectively. Four pitfall traps were deployed at each site. In 2019, each site was sampled in May, June, and July, whereas in 2020, each site was sampled in June and August. In 2019, the numbers of predatory heteropterans were two times greater in bermudagrass (Cynodon dactylon L.) than in zoysiagrass (Zoysia spp.). The numbers of predatory mirids, Spanogonicus albofasciatus (Reuter), and carabids significantly decreased with increases in turfgrass height. In 2020, the abundance of staphylinids increased as the thatch thickness increased. The abundance of Sphenophorus spp. adults were significantly greater in bermudagrass than in zoysiagrass in 2020 and were more abundant in the denser turfgrasses in both years. The predatory arthropods were positively correlated with increased densities of cicadellids, whereas predatory mirids were positively associated with cicadellids, delphacids, and chrysomelids. These results have implications on management of arthropod pests in sod farms as abundance of beneficial arthropods are influenced by turfgrass factors.
Connectome-based prediction of eating disorder-associated symptomatology

Abstract Background Despite increasing knowledge on the neuroimaging patterns of eating disorder (ED) symptoms in non-clinical populations, studies using whole-brain machine learning to identify connectome-based neuromarkers of ED symptomatology are absent. This study examined the association of connectivity within and between large-scale functional networks with specific symptomatic behaviors and cognitions using connectome-based predictive modeling (CPM). Methods CPM with ten-fold cross-validation was carried out to probe functional networks that were predictive of ED-associated symptomatology, including body image concerns, binge eating, and compensatory behaviors, within the discovery sample of 660 participants. The predictive ability of the identified networks was validated using an independent sample of 821 participants. Results The connectivity predictive of body image concerns was identified within and between networks implicated in cognitive control (frontoparietal and medial frontal), reward sensitivity (subcortical), and visual perception (visual). Crucially, the set of connections in the positive network related to body image concerns identified in one sample was generalized to predict body image concerns in an independent sample, suggesting the replicability of this effect. Conclusions These findings point to the feasibility of using the functional connectome to predict ED symptomatology in the general population and provide the first evidence that functional interplay among distributed networks predicts body shape/weight concerns.
Co-occurrence of online and offline bullying and sexual harassment among youth in Sweden: Implications for studies on victimization and health a short communication

ABSTRACT Studies of co-occurrence of online and offline victimisation of bullying and sexual harassment and its associations to mental health outcomes among youth are scarce. To inform future study designs, the aim of this brief communication was to map co-occurrence of online and offline bullying and sexual harassment victimisation among adolescents. Data were collected in 2011 in nine schools in Northern Sweden, n = 1193 (boys = 566; girls = 627). Absolute and relative frequencies were calculated to find combinations of victimisation: one, two, three, or four forms. Reflecting a picture of the early days of online victimisation, in total fifty seven percent (57%) of adolescents were victimised. Single occurrence victimisation was 21.2% (offline sexual harassment was most common irrespective of gender), showing that most youths were victimised in a co-occurrence of two or more forms. Seven percent (7%) were victimised by all four forms of victimisation. Offline sexual harassment victimisation was present in the most common co-occurrences. Directions for future studies of victimisation and its associations to mental health outcomes are discussed.
Mercury's geochronology revised by applying Model Production Function to Mariner 10 data: Geological implications

Model Production Function (MPF) chronology uses dynamic models of the Main Belt Asteroids (MBAs) and Near Earth Objects (NEOs) to derive the impactor flux to a target body. This is converted into the crater size‐frequency‐distribution for a specific planetary surface, and calibrated using the radiometric ages of different regions of the Moon's surface. This new approach has been applied to the crater counts on Mariner 10 images of the highlands and of several large impact basins on Mercury. MPF estimates for the plains show younger ages than those of previous chronologies. Assuming a variable uppermost layering of the Hermean crust, the age of the Caloris interior plains may be as young as 3.59 Ga, in agreement with MESSENGER results that imply that long‐term volcanism overcame contractional tectonics. The MPF chronology also suggests a variable projectile flux through time, coherent with the MBAs for ancient periods and then gradually comparable also to the NEOs.
Consensus-Based Recommendations on Priority Activities to Address Acute Kidney Injury in Children

IMPORTANCE Increasing evidence indicates that acute kidney injury (AKI) occurs frequently in children and young adults and is associated with poor short-term and long-term outcomes. Guidance is required to focus efforts related to expansion of pediatric AKI knowledge. OBJECTIVE To develop expert-driven pediatric specific recommendations on needed AKI research, education, practice, and advocacy. EVIDENCE REVIEW At the 26th Acute Disease Quality Initiative meeting conducted in November 2021 by 47 multiprofessional international experts in general pediatrics, nephrology, and critical care, the panel focused on 6 areas: (1) epidemiology; (2) diagnostics; (3) fluid overload; (4) kidney support therapies; (5) biology, pharmacology, and nutrition; and (6) education and advocacy. An objective scientific review and distillation of literature through September 2021 was performed of (1) epidemiology, (2) risk assessment and diagnosis, (3) fluid assessment, (4) kidney support and extracorporeal therapies, (5) pathobiology, nutrition, and pharmacology, and (6) education and advocacy. Using an established modified Delphi process based on existing data, workgroups derived consensus statements with recommendations. FINDINGS The meeting developed 12 consensus statements and 29 research recommendations. Principal suggestions were to address gaps of knowledge by including data from varying socioeconomic groups, broadening definition of AKI phenotypes, adjudicating fluid balance by disease severity, integrating biopathology of child growth and development, and partnering with families and communities in AKI advocacy. CONCLUSIONS AND RELEVANCE Existing evidence across observational study supports further efforts to increase knowledge related to AKI in childhood. Significant gaps of knowledge may be addressed by focused efforts.
Factors associated with in-hospital mortality of patients admitted to an intensive care unit in a tertiary hospital in Malawi

Objective To determine factors associated with in-hospital death among patients admitted to ICU and to evaluate the predictive values of single severely deranged vital signs and several severity scoring systems. Methods A combined retrospective and prospective cohort study of patients admitted to the adult ICU in a tertiary hospital in Malawi was conducted between January 2017 and July 2019. Predefined potential risk factors for in-hospital death were studied with univariable and multivariable logistic regression models, and the performance of severity scores was assessed. Results The median age of the 822 participants was 31 years (IQR 21–43), and 50% were female. Several factors at admission were associated with in-hospital mortality: the presence of one or more severely deranged vital signs, adjusted odds ratio (aOR) 1.9 (1.4–2.6); treatment with vasopressor aOR 2.3 (1.6–3.4); received cardiopulmonary resuscitation aOR 1.7 (1.2–2.6) and treatment with mechanical ventilation aOR 1.5 (1.1–2.1). Having had surgery had a negative association with in-hospital mortality aOR 0.5 (0.4–0.7). The predictive accuracy of the severity scoring systems had varying sensitivities and specificities, but none were sufficiently accurate to be clinically useful. Conclusions In conclusion, the presence of one or more severely deranged vital sign in patients admitted to ICU may be useful as a simple marker of an increased risk of in-hospital death.