Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential anti-inflammatory properties of chitosan-based cryogel scaffolds depending on chitosan/gelatin ratio

Abstract Chitosan/gelatine-based materials have been widely used as biocompatible scaffolds in the tissue engineering field. Chitosan suppresses the inflammatory activities of macrophages whereas gelatine induces inflammatory cytokine production by these cells. Cryogel form of the scaffolds created an effect that was mostly dominated by chitosan activity. Since independent of chitosan to gelatine ratio, the cryogels eliminated the inflammatory cytokine production by the activated macrophages. This will enable suppression of inflammatory reactions by macrophages during implant procedure while enabling a nest of the matrix for the macrophages to reside. Determining the immunomodulatory effect of these materials during the decay is crucial to assess their biocompatibility and safety. Our results suggest that when the chitosan ratio was higher than that of gelatine the materials had anti-inflammatory activity in their powder forms based on TNFα production levels by LPS activated macrophages, whereas higher gelatine to chitosan ratio eliminated this effect. To our knowledge, this is the first study to assess the powder vs. gel forms of the chitosan/gelatine-based materials for their immunomodulatory potentials as well as how the ratio of chitosan to gelatine might affect these materials immunomodulatory effects on the activated macrophages. HIGHLIGHTS Chitosan/gelatin composite cryogels have anti-inflammatory activities. Different ratios of chitosan to gelatin content altered the immunomodulatory activities. They can be safely and effectively used as implant materials for tissue engineering applications. They will also reduce the use of anti-inflammatory drugs during implantation.
RASSF1A disrupts the NOTCH signaling axis via SNURF/RNF4‐mediated ubiquitination of HES1

RASSF1A promoter methylation has been correlated with tumor dedifferentiation and aggressive oncogenic behavior. Nevertheless, the underlying mechanism of RASSF1A‐dependent tumor dedifferentiation remains elusive. Here, we show that RASSF1A directly uncouples the NOTCH‐HES1 axis, a key suppressor of differentiation. Interestingly, the crosstalk of RASSF1A with HES1 occurs independently from the signaling route connecting RASSF1A with the Hippo pathway. At the molecular level, we demonstrate that RASSF1A acts as a scaffold essential for the SUMO‐targeted E3 ligase SNURF/RNF4 to target HES1 for degradation. The reciprocal relationship between RASSF1A and HES1 is evident across a wide range of human tumors, highlighting the clinical significance of the identified pathway. We show that HES1 upregulation in a RASSF1A‐depleted environment renders cells non‐responsive to the downstream effects of γ‐secretase inhibitors (GSIs) which restrict signaling at the level of the NOTCH receptor. Taken together, we report a mechanism through which RASSF1A exerts autonomous regulation of the critical Notch effector HES1, thus classifying RASSF1A expression as an integral determinant of the clinical effectiveness of Notch inhibitors.
Global Estimates of Rotavirus Hospitalizations Among Children Below 5 Years in 2019 and Current and Projected Impacts of Rotavirus Vaccination.

BACKGROUND Rotavirus vaccine impact on rotavirus hospitalizations is not well documented globally. We performed a systematic review to estimate the number of rotavirus hospitalizations that (1) occur annually, (2) are currently prevented by rotavirus vaccines, and (3) could be prevented with improved vaccine coverage and universal vaccine introduction. METHODS We systematically reviewed articles indexed in the PubMed database published from January 1, 2000, to December 31, 2019. We included all primary peer-reviewed studies with rotavirus hospitalization rates for children below 5 years that reported data prior to vaccine introduction, utilized at least one continuous year of data collection, and collected hospitalization data after 2000 using active surveillance. We grouped pre-vaccine country estimates by childhood mortality strata and calculated the median rate among each group. We then assigned the mortality stratum-specific hospitalization rates to each country and calculated the number of rotavirus hospitalizations by country, mortality strata, and World Health Organization region. RESULTS Our search strategy identified 4590 manuscripts, of which 32 were included in the final dataset. In 2019, an estimated 1 760 113 (interquartile range [IQR]: 1 422 645-2 925 372) rotavirus hospitalizations occurred globally, with 524 871 (IQR: 415 987-814 835) prevented by rotavirus vaccination. With universal introduction of rotavirus vaccines and increased vaccine coverage, we estimate that an additional 751 609 (IQR: 607 671-1 318 807) rotavirus hospitalizations can be prevented annually. CONCLUSIONS This analysis highlights the continued burden of rotavirus hospitalizations among children below 5 years. A large, preventable proportion of this burden could be eliminated by expanding introductions to new countries and increasing rotavirus vaccine coverage to levels seen with other childhood vaccinations.
The mitochondrial antioxidant SS-31 attenuated lipopolysaccharide-induced apoptosis and pyroptosis of nucleus pulposus cells via scavenging mitochondrial ROS and maintaining the stability of mitochondrial dynamics

Abstract Evidence has shown that effects from inflammation and mitochondrial dysfunction lead to pyroptosis and apoptosis of nucleus pulposus (NP) cells. Damaged mitochondria release dangerous molecules such as reactive oxygen species (ROS), activating the NLRP3 inflammasome. SS-31 is a mitochondria-targeting peptide that has been used in the treatment of many diseases by scavenging ROS and ameliorating mitochondrial function. This study found that SS-31 ameliorated lipopolysaccharide (LPS)-induced loss of cell viability, ROS production, and apoptosis in NP cells. Moreover, mitochondrial dynamics and ATP synthesis were restored on pretreatment with SS-31 compared with the LPS group. For the molecular mechanism research, SS-31 stabilized mitochondrial morphology and inhibited the activation of the NF-κB pathway and the activation of the NLRP3 inflammasome. To evaluate whether the inhibition of NLRP3 inflammasome activation by SS-31 is dependent on the clearance of mitochondrial ROS, we comparatively analyzed the activation of NLRP3 inflammasome in NP cells pretreated with SS-31 and the ROS scavenger N-acetyl-L-cysteine (NAC). The results indicate that SS-31 could inhibit NLRP3 inflammasome activation by limiting the production of mitochondrial ROS. To sum up, our results revealed that SS-31 inhibits LPS-induced apoptosis, pyroptosis, and inflammation in NP cells via scavenging ROS and maintaining the stability of mitochondrial dynamics, which could be considered a promising therapeutic intervention for disk degeneration.
Revision Anterior Cruciate Ligament Reconstruction Using Bone-Patellar Tendon-Bone Graft Combined With Modified Lemaire Technique Versus Hamstring Graft Combined With Anterolateral Ligament Reconstruction: A Clinical Comparative Matched Study With a Mean Follow-up of 5 Years From The SANTI Study Gro

Background: Additional lateral extra-articular tenodesis (LET) has recently been correlated with improved clinical outcomes and reduced failure rates in revision anterior cruciate ligament (ACL) reconstruction (ACLR). However, no data are available on clinical outcomes and reoperation after revision ACLR using different LET procedures. Purpose: To compare the clinical outcomes of ACL + anterolateral ligament (ALL) reconstruction using hamstring tendon graft (HT-ALL) and a bone–patellar tendon–bone (BPTB) graft + modified Lemaire tenodesis procedure (BPTB-Lemaire) in the setting of revision ACLR and to determine whether ALL reconstruction is associated with an increased rate of adverse outcomes when compared with a modified Lemaire tenodesis procedure. Study Design: Cohort study; Level of evidence, 3. Methods: Descriptive data and clinical outcomes were prospectively collected from patients who underwent revision ACLR with LET between 2009 and 2018 with a minimum follow-up of 2 years. Patients with an HT autograft combined with ALL reconstruction (HT-ALL group) were matched in a 1:1 propensity ratio to patients with a BPTB autograft combined with a modified Lemaire LET procedure (BPTB-Lemaire group). The evaluated parameters included complications and reoperations; knee laxity tests; return to sports; and various scores, including the Lysholm knee score, Tegner activity scale, Anterior Cruciate Ligament Return to Sport After Injury scale, Marx activity rating scale, International Knee Documentation Committee subjective knee evaluation form, and Knee injury and Osteoarthritis Outcome Score. Results: In total, 36 matched pairs were included in the analysis. The mean follow-up durations for the BPTB-Lemaire and HT-ALL groups were 56 ± 35 and 57 ± 23 months, respectively (P = .91). No significant differences were found in graft rupture rate (HT-ALL, 0%; BPTB-Lemaire, 11.1%; P = .13) or reoperations (HT-ALL, 8.3%; BPTB-Lemaire, 22.2%; P = .23). No specific complications with regard to LET were noted in either group. Additionally, there were no significant differences in knee laxity parameters, return to sports, or clinical scores between the groups at the final follow-up, except for the Tegner activity scale score (HT-ALL, 6.4; BPTB-Lemaire, 7.3; P = .03). HT-ALL was associated with a shorter surgical time (41.4 vs 59.8 minutes; P < .0001). Conclusion: HT-ALL was at least equivalent, in terms of clinical outcomes, to the more commonly performed procedure, BPTB-Lemaire. Performing ALL reconstruction in the setting of revision ACLR is therefore safe and effective.
A Computationally Efficient Lower Limb Finite Element Musculoskeletal Framework Directly Driven Solely by Inertial Measurement Unit Sensors.

Finite element musculoskeletal (FEMS) approaches using concurrent musculoskeletal and finite element models driven by motion data such as marker-based motion trajectory can provide insight into the interactions between the knee joint secondary kinematics, contact mechanics, and muscle forces in subject-specific biomechanical investigations. However, these data-driven FEMS systems have two major disadvantages that make them challenging to apply in clinical environments: they are computationally expensive and they require expensive and inconvenient equipment for data acquisition. In this study, we developed an FEMS model of the lower limb driven solely by inertial measurement unit sensors that includes the tissue geometries of the entire knee joint and combines muscle modeling and elastic foundation theory-based contact analysis of knee into a single framework. The model requires only the angular velocities and accelerations measured by the sensors as input, and the target outputs (knee contact mechanics, secondary kinematics, and muscle forces) are predicted from the convergence results of iterative calculations of muscle force optimization and knee contact mechanics. To evaluate its accuracy, the model was compared with in vivo experimental data during gait. The maximum contact pressure (12.6 MPa) in the rigid body contact analysis occurred on the medial side of the cartilage at the maximum loading response. The proposed computationally efficient framework drastically reduced the computational time (97.5% reduction) in comparison with the conventional deformable finite element analysis. The developed framework combines measurement convenience and computational efficiency and shows promise for clinical applications.
Prediction of Machine Deterioration Using Vibration Based Fault Trends and Recurrent Neural Networks

High market competition for sales requires companies to reduce the cost of production if they are to maintain their market shares. Since the cost of maintenance contributes a substantial portion of the production cost, companies must budget maintenance effectively. Machine deterioration prognosis can decrease the cost of maintenance by minimizing the loss of production due to machine breakdown and avoiding the overstocking of spare parts. A new prognostic method is described in this paper which has been developed to forecast the rate of machine deterioration using recurrent neural networks. From tests applying the method to the prediction of nonlinear sunspot activities and vibration based fault trends of several industrial machines, the results have shown that the method is promising. It not only evaluates the seriousness of damage caused by faults, but also forecasts the remaining life span of defective components.
The Impact of Intimate Partner Violence on Young Women’s Educational Well-Being: A Systematic Review of Literature

Research highlights that romantic relationships of young people are not all ‘puppy love’ but can be also abusive. Intimate partner violence (IPV) is a gendered phenomenon as it primarily affects women who are at a higher risk of more severe forms of violence and also suffer more severe consequences than young men. IPV leads to substantial negative outcomes such as mental health decline, economic insecurity and/or academic underachievement. Particularly for young females, education is a powerful protective factor against re-victimisation and economic dependence which often forces women to remain trapped in abusive relationships. This review was conducted to integrate and summarise research available on IPV and its impact on young women’s educational well-being to fill a significant gap in the literature. Under the guidance of PRISMA, terms related to the criteria of young women aged 10–24, IPV and education were searched in the databases EBSCO, PsycINFO, Scopus, ProQuest and CINAHL. While the initial search yielded 6005 articles, we were left with only 10 articles for the analysis. In summary, the evidence suggests that females tend to display issues around concentration, absenteeism and academic disengagement, as well as decline in performance such as failing grades and higher drop out rates.
Hemostatic efficacy of pathogen‐reduced platelets in children undergoing cardiopulmonary bypass

Pediatric patients undergoing cardiopulmonary bypass (CPB) often require blood component transfusions. Pathogen‐reduction (PR) of platelets reduces the risk of microbial contamination; however, its effect on hemostatic efficacy in this population is unclear. This study sought to characterize the hemostatic efficacy of PR platelets in children undergoing CPB.
Prevalence of users of medication targeting obstructive lung disease, the reasons for prescriptions and the use of spirometry in Upernavik Health Center in the period from 2011-2016, a retrospective observational study as basis for future quality development

ABSTRACT Chronic obstructive pulmonary disease (COPD) is a health problem globally. Smoking is a risk factor. In Greenland over 50% are smokers. Upernavik Healthcare Center serves the town of Upernavik and its 9 remote settlements. Many patients were treated with medications targeting obstructive pulmonary disease (ATC code R03). This retrospective observational study estimated the prevalence of users of R03 medication aged 50 years or above, investigated if spirometry was performed, the reason for prescription and smoking status. The study is based on review of data from the electronic medical journal. Permanent residents with prescriptions of R03 medication within a period of 5 years were included. Reasons for prescription and smoking status was registrated. The prevalence of users of R03 medications was 7,6%. 37,8% had a spirometry performed. The reason for prescribing varied from no reason to COPD-like. R03 medications were prescribed years before spirometry. 37,8% of the patients were smokers. Prevalence of users was comparable to other studies in Greenland. There was lack of spirometry and a discrepancy to guidelines. Patients had prescriptions of R03 medication years without a specific diagnosis. This warrant a new strategy for identification, increase of spirometry and treatment of patients with COPD to be developed.
Pre‐operative predictive factors for gangrenous cholecystitis at an Australian quaternary cardiothoracic centre

The aim of this study was to determine associations between pre‐operative factors and gangrenous cholecystitis. Gangrenous cholecystitis represents a severe spectrum of cholecystitis and is associated with an increased risk of complications. Early recognition and diagnosis of gangrenous cholecystitis can optimize prioritization for surgery and improve outcomes.
The PKCγ neurons in anterior cingulate cortex contribute to the development of neuropathic allodynia and pain-related emotion

Background While the PKCγ neurons in spinal dorsal horn play an indispensable part in neuropathic allodynia, the exact effect of PKCγ neurons of brain regions in neuropathic pain remains elusive. Mounting research studies have depicted that the anterior cingulate cortex (ACC) is closely linked with pain perception and behavior, the present study was designed to investigate the contribution of PKCγ neurons in ACC to neuropathic allodynia and pain-related emotion in newly developed Prkcg-P2A-Tdtomato mice. Methods The c-fos expression in response to innocuous stimulation was used to monitor the activity of PKCγ in CCI (chronic constriction injury of the sciatic nerve) induced neuropathic pain condition. Activating or silencing ACC PKCγ neurons by chemogenetics was applied to observe the changes of pain behavior. The excitability of ACC PKCγ neurons in normal and CCI mice was compared by patch-clamp whole-cell recordings. Results The PKCγ-Tdtomato neurons were mainly distributed in layer III-Vof ACC. The Tdtomato was mainly expressed in ACC pyramidal neurons demonstrated by intracellular staining. The c-fos expression in ACC PKCγ neurons in response to innocuous stimulation was obviously elevated in CCI mice. The patch clamp recordings showed that ACC PKCγ-Tdtomato neurons were largely activated in CCI mice. Chemogenetic activation of ACC PKCγ neurons in Prkcg-icre mice induced mechanical allodynia and pain-related aversive behavior, conversely, silencing them in CCI condition significantly reversed the mechanical allodynia and pain-related place aversive behavior. Conclusion We conclude that the PKCγ neurons in ACC are closely linked with neuropathic allodynia and pain-related emotional behaviors.
Evidence for a vocal signature in the rat and its reinforcing effects

While the term “language” is used for human and non-human primates, “vocal communication” is rather used for rodents or other species. The main difference is that there is, to date, no evidence for a vocal signature in the well-known 50- and 22-kHz ultrasonic vocalizations (USV) emitted by rats. Here, we show that rats can recognize the identity of the USV emitter since they self-administer preferentially playback of 50-kHz USV emitted by a stranger rat over those emitted by their cage-mate. In a second experiment, we show that the familiarity with the USV emitter also modulate the effect of USV playback during cocaine self-administration, since only stranger, but not familiar, 50-kHz USV decrease drug intake. Finally, to study the neurobiological substrate of those processes, we have tested the effects of the subthalamic nucleus (STN) lesion on these various conditions. STN-lesioned rats did not lever press much for any USV playback, whatever their emotional valence, nor did they seem able to differentiate familiar from stranger peer. Advocating for the existence of a vocal signature in rats, these results highlight the importance of ultrasonic communication in socio-affective influence of behavior, such as the influence of proximal social factors on drug consumption and confirm the role of the subthalamic nucleus on this influence.
Running Wild: Environmental Attitudes of Long-Distance Runners in the Icelandic Highlands

An increasing number of sports events are taking place in wilderness and mountain areas. The Laugavegur Ultra Marathon (LUM) is one such event. It follows the 55-km (34.2-mile) route of the Laugavegur trail in the southern Icelandic highlands. The trail has been selected as one of the world's most scenic long-distance hiking trails by many of the world's leading travel media. This paper focuses on LUM runners' attitudes toward environmental issues to identify their values as a group and to examine whether there are different attitudes between Icelandic and international runners. An online survey was distributed to all participants finishing the race in 2018, a total of 553 runners; the response rate was 45.2%. The results reveal that most runners participating in LUM were running for their own personal goals and challenges, using the trail's wilderness setting as a driver to reach their target. As a group, the runners had relatively ecocentric orientations. With regard to environmental values, however, there was a large difference between Icelandic and international runners. The Icelandic runners significantly favored anthropocentric orientations, preferring more infrastructure and services along the route, compared with international runners, who preferred the wild character of the route. When it comes to planning sports events in natural settings, such as mountain marathons in wilderness areas, it is important to raise the environmental awareness of both users and event organizers. Moreover, to sustain the wilderness character of the trail, it is beneficial to attract more runners who have an ecocentric view.
Cooperative Communications Based Quadrature Space Shift Keying: An Information–Theoretic Approach

In this paper, we consider the analysis of mutual information and system capacity in cooperative half–duplex amplify and forward (AF) relaying system based multiple– input multiple–output (MIMO)-quadrature space shift keying (QSSK). To this end, we first derive an analytical expression for theoretical mutual information and then it is employed to derive a closed–form upper bound expression for the system capacity. Interestingly, the derived capacity expression shows that the system capacity is independent of channel distribution. Some representative numerical examples are presented to study the impact of different system parameters on the mutual information and system capacity. As a benchmark, the results for a similar system employing MIMO-space shift keying (SSK) are provided. The results demonstrate that the spectral efficiency can be enhanced as the number of transmit antenna increases, whereas it degrades as the number of relays increases. Additionally, the results show that increasing the number of relays leads to insignificant degradation in the system capacity.
Factors affecting the quality of work-life of nurses: a correlational study

Background Quality of work-life is an important factor in the recruitment and retention of the nursing workforce and their work productivity. Aims This study aimed to identify factors that affect the quality of work-life of nurses. Methods In this correlational study, 239 nurses employed in a tertiary teaching hospital in Iran completed the study questionnaires, including the quality of work-life questionnaire. The associations between potential independent variables and quality of work-life were examined using multivariate regression analysis. Results Participants were mostly women (80.33%) and within the age range of 30–45 years (71.54%). Working in other hospitals, having a second job and health information-seeking behaviours were significant predictors of quality of work-life, explaining 28.68% of the total variance in quality of work-life of nurses. Conclusions Improving working standards may prevent nurses from working extra hours or in different occupations. This, along with facilitating the health information-seeking behaviours of nurses, may help improve their quality of work-life by maintaining a better work-life balance and acquiring knowledge and skills that can help with effective management of work and life commitments.