Butyribacter intestini a butyric acid-producing bacterium of the family Lachnospiraceae isolated from human faeces, and reclassification of Acetivibrio ethanolgignens as Acetanaerobacter ethanolgignens

a bacterium of the family Lachnospiraceae isolated from human faeces, reclassification of Acetivibrio ethanolgignens Acetanaerobacter Abstract 41 A novel, non-motile, Gram-stain-positive, non-spore-forming, obligate anaerobic 42 bacterium, designated strain TF01-11 T , was isolated from human faeces. The isolate was 43 characterized by phylogenetic and phenotypic properties, as well as by determination of its 44 whole genome sequence. The growth temperature and pH ranges were 30–42 °C and 6.0–8.5, 45 respectively. The end products of glucose fermentation were butyric acid and a small 46 amount of acetic acid. The genome was estimated to be 3.61Mbp with G+C content of 36.79 47 mol%. Genes related to biosynthesis of diaminopimelic acid, polar lipids, polyamines, 48 teichoic and lipoteichoic acids were present. The predominant fatty acids were C 16:0 (37.9 %), 49 C 14:0 (16.4 %), C 13:0 OH/iso-C 15:1 H (11.1 %) and C 18:1 ω 9 c (10.6 %). Phylogenetic analyses 50 based on 16S rRNA gene sequences, the isolate was a member of family Lachnospiraceae , 51 with the highest sequence similarity to the type strain of Roseburia intestinalis DSM 14610 T 52 at 92.18 % followed by Acetivibrio ethanolgignens ATCC 33324 T at 91.99 %. The average 53 nucleotide identity (ANI) calculated for the genomes between strain TF01-11 T and these 54 closest relatives were 70.5 % and 68.1 %. Based on results of phenotypic characteristics and 55 genotypic properties presented in this study, strain TF01-11 T represent a novel species in a 56 new genus, for which the name Butyribacter intestini gen. nov., sp. nov. is proposed. The type 57 strain of the type species is TF01-11 T (CGMCC 1.5203 T = CGMCC 10984 T = DSM 105140 T ). -adenosylhomocysteine nucleosidase (EC (1 gene), ABC transporter, periplasmic spermidine putrescine-binding protein (TC (1 gene), arginine decarboxylase (EC 4.1.1.19) lysine decarboxylase (EC 4.1.1.18) (1 gene), carbamate kinase (EC 2.7.2.2) (1 gene), putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) (1 gene), spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) (1 gene), and spermidine putrescine ABC transporter permease component potC (TC.3.A.1.11.1) (1 gene) genes/protein responsible for biosynthesis of teichoic lipoteichoic acids, including 2- C -methyl- D -erythritol 4-phosphate cytidylyltransferase (EC (2 genes), glycerophosphotransferase (2 genes), membrane protein the export O-antigen, teichoic acid lipoteichoic acids (1 gene), teichoic acid biosynthesis protein GgaB (1 gene), (EC (1 gene), teichoic acid (EC (3 genes), teichoic acid glycosylation gene), teichoic protein TagG gene), genes/protein biosynthesis of Cells are about 0.5–0.9 μ m long and 1.5–2.5 μ m wide, occurring singly, in pairs, and often in short chains. Colonies are yellowish-white, circular, convex, smooth, translucent, and 0.5 to 1.5 273 mm in diameter on PYG plate after 2–3 days of cultivation. The fermentation products is acetic acid, ethanol, hydrogen, and carbon dioxide. Positive result in tests for leucine arylamidase activity, but negative for acid phosphatise, α -glucosidase activities, alkaline phosphatase, esterase 276 (C4), esterase lipase (C8), lipase (C14), valine arylamidase, cystine arylamidase, trypsin, α - chymotrypsin, naphthol-AS-BI-phosphohydrolase, α -galactosidase, β -galactosidase, β - 278 glucuronidase, β -glucosidase, N -acetyl- β -glucosaminidase, α -mannosidase and β -fucosidase. Acid 279 is produced from glucose, mannose, lactose, maltose, salicin, mannose, cellobiose (weak positive), raffinose (weak positive), fructose, galactose, mannitol, pyruvate and starch (weak positive), but 281 not from xylose, arabinose, glycerol, melezitose, sorbitol, rhamnose, trehalose, adonitol, lactate, raffinose, ribose and sucrose. Indole is not produced. Gelatin is liquefied. Aesculin is not 283 hydrolysed.