Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessment of the individual and compounding effects of marginalization factors on injury severity, discharge location, recovery, and employment outcomes at 1 year after traumatic brain injury

Objective The aim of this study is to examine the effect of eight distinct marginalized group memberships and explore their compounding effect on injury severity, recovery, discharge location, and employment outcomes 1-year after traumatic brain injury (TBI). Methods Individuals with medically confirmed, complicated mild-severe TBI (N = 300) requiring inpatient rehabilitation care between the ages of 18 and 65 were recruited at two urban (public and private) health systems between 2013 and 2019. Data were collected from self-report and medical record abstraction. Marginalized group membership (MGM) includes racial and ethnic minority status, less than a high school diploma/GED, limited English proficiency, substance abuse, homelessness, psychiatric hospitalizations, psychiatric disorders, and incarceration history. Membership in four or more of these groups signifies high MGM. In addition, these factors were explored individually. Unadjusted and adjusted linear and logistic regressions and Kruskal–Wallis tests were used to assess the associations of interest in RStudio. Results After adjusting for age, sex, and cause of injury, compared to TBI patients with low MGM, those with high MGM experience significantly longer post-traumatic amnesia (95% CI = 2.70, 16.50; p = 0.007) and are significantly more likely to have a severe TBI (per the Glasgow-Coma Scale) (95% CI = 1.70, 6.10; p ≤ 0.001) than a complicated mild-moderate injury. Individuals with high MGM also are significantly less likely to be engaged in competitive paid employment 1 year after injury (95% CI = 2.40, 23.40; p = 0.001). Patients with high MGM are less likely to be discharged to the community compared to patients with low MGM, but this association was not significant (95% CI = 0.36, 1.16; p = 0.141). However, when assessing MGMs in isolation, certain associations were not significant in unadjusted or adjusted models. Conclusion This exploratory study's findings reveal that when four or more marginalization factors intersect, there is a compounding negative association with TBI severity, recovery, and employment outcomes. No significant association was found between high MGM and discharge location. When studied separately, individual MGMs had varying effects. Studying marginalization factors affecting individuals with TBI has critical clinical and social implications. These findings underline the importance of addressing multidimensional factors concurrent with TBI recovery, as the long-term effects of TBI can place additional burdens on individuals and their economic stability.
The prognostic value of systemic immune-inflammation index in surgical esophageal cancer patients: An updated meta-analysis

Purpose To identify the prognostic role of systemic immune-inflammation index (SII) in esophageal cancer patients receiving operation. Methods The PubMed, EMBASE, Web of Science, Cochrane Library, WanFang and CNKI electronic databases were searched up to February 17, 2022 for relevant studies. The hazard ratios (HRs) and 95% confidence intervals (CIs) were combined to assess the association between SII and prognosis in surgical esophageal cancer patients. The primary outcome was overall survival (OS) and secondary outcomes were progression-free survival (PFS) and cancer-specific survival (CSS). All statistical analyses were conducted by STATA 15.0 software. Results A total of nine retrospective studies involving 3,565 participates were included. The pooled results indicated that high SII was significantly related with poor OS (HR = 1.58, 95% CI: 1.23–2.02, P < 0.001). However, subgroup analysis based on pathological type demonstrated that high SII was an independent predictor for poor OS only in esophageal squamous cell carcinoma (ESCC) patients (HR = 1.72, 95% CI: 1.34–2.21, P < 0.001). Besides, SII was also significantly associated with poor PFS (HR = 1.94, 95% CI: 1.61–2.35, P < 0.001) and CSS (HR = 1.44, 95% CI: 1.04–1.99, P = 0.027) in ESCC patients. Conclusion The SII could serve as an independent prognostic factor in surgical ESCC patients and higher SII was related with worse survival. However, more prospective high-quality studies are still needed to verify above findings.
Building community capacities in evaluating rural IT projects: Success strategies from the LEARNERS Project

Given the current emphasis on the benefits of communication and information technologies (C&IT) for sustainable rural community development, effective evaluations of C&IT initiatives are increasingly important. This paper presents outcomes of a project that aimed to build capacities of people in two Australian rural communities to evaluate C&IT initiatives. The project’s participatory action research and participatory evaluation methods were effective in increasing skills and knowledge, and facilitating various forms of empowerment. However, some limitations and disempowering effects and barriers to participation were identified. Based on our critical reflections, we present strategies for successful community capacity building projects and sustainable C&IT initiatives in rural areas.
Comprehensive landscape of the ST3GAL family reveals the significance of ST3GAL6-AS1/ST3GAL6 axis on EGFR signaling in lung adenocarcinoma cell invasion

Sialylation aberration has been implicated in lung cancer development by altering signaling pathways. Hence, it is urgent to identify key sialyltransferases in the development of lung adenocarcinoma (LUAD), which is a common malignant subtype of non-small cell lung cancer. Herein, by systematically investigating the expression levels of ST3GAL family members in several public databases, we consistently found the frequent downregulation of ST3GAL6 in LUAD samples. Its downregulation is significantly negatively associated with stage, and significantly reduced in proximal-proliferative molecular subtype and predicts poor clinical outcomes. By protein–protein interaction network analysis and validation, we found that ST3GAL6 deficiency promotes LUAD cell invasiveness with the activated EGFR/MAPK signaling, accompanied by the elevated expression levels of matrix metalloproteinases 2 and 9, which can be partially reversed by EGFR inhibitor, gefitinib. Additionally, the ST3GAL6 level was positively regulated by ST3GAL6-AS1, an antisense long non-coding RNA to its host gene. The downregulation of ST3GAL6-AS1 also heralds a worse prognosis in LUAD patients and promotes LUAD cell invasiveness, recapitulating the function of its host gene, ST3GAL6. Altogether, ST3GAL6-AS1-regulated ST3GAL6 is a frequently downregulated sialyltransferase in LUAD patients and negatively regulates EGFR signaling, which can serve as a promising independent prognostic marker in LUAD patients.
Integrated analysis of inflammatory response subtype-related signature to predict clinical outcomes, immune status and drug targets in lower-grade glioma

Background: The inflammatory response in the tumor immune microenvironment has implications for the progression and prognosis in glioma. However, few inflammatory response-related biomarkers for lower-grade glioma (LGG) prognosis and immune infiltration have been identified. We aimed to construct and identify the prognostic value of an inflammatory response-related signature, immune infiltration, and drug targets for LGG. Methods: The transcriptomic and clinical data of LGG samples and 200 inflammatory response genes were obtained from public databases. The LGG samples were separated into two inflammatory response-related subtypes based on differentially expressed inflammatory response genes between LGG and normal brain tissue. Next, inflammatory response-related genes (IRRGs) were determined through a difference analysis between the aforementioned two subtypes. An inflammatory response-related prognostic model was constructed using IRRGs by using univariate Cox regression and Lasso regression analyses and validated in an external database (CGGA database). ssGSEA and ESTIMATE algorithms were conducted to evaluate immune infiltration. Additionally, we performed integrated analyses to investigate the correlation between the prognostic signature and N 6-methyladenosine mRNA status, stemness index, and drug sensitivity. We finally selected MSR1 from the prognostic signature for further experimental validation. Results: A total of nine IRRGs were identified to construct the prognostic signature for LGG. LGG patients in the high-risk group presented significantly reduced overall survival than those in the low-risk group. An ROC analysis confirmed the predictive power of the prognostic model. Multivariate analyses identified the risk score as an independent predictor for the overall survival. ssGSEA revealed that the immune status was definitely disparate between two risk subgroups, and immune checkpoints such as PD-1, PD-L1, and CTLA4 were significantly expressed higher in the high-risk group. The risk score was strongly correlated with tumor stemness and m6A. The expression levels of the genes in the signature were significantly associated with the sensitivity of tumor cells to anti-tumor drugs. Finally, the knockdown of MSR1 suppressed LGG cell migration, invasion, epithelial–mesenchymal transition, and proliferation. Conclusion: The study constructed a novel signature composed of nine IRRGs to predict the prognosis, potential drug targets, and impact immune infiltration status in LGG, which hold promise for screening prognostic biomarkers and guiding immunotherapy for LGG.
Recent developments on the application of molecular probes in multiple myeloma: Beyond [18F]FDG

Multiple myeloma (MM) is a neoplastic plasma cell proliferative disorder characterized by various osteolytic bone destruction as a radiological morphological marker. Functional imaging, particularly nuclear medicine imaging, is a promising method to visualize disease processes before the appearance of structural changes by targeting specific biomarkers related to metabolism ability, tumor microenvironment as well as neoplastic receptors. In addition, by targeting particular antigens with therapeutic antibodies, immuno-PET imaging can support the development of personalized theranostics. At present, various imaging agents have been prepared and evaluated in MM at preclinical and clinical levels. A summary overview of molecular functional imaging in MM is provided, and commonly used radiotracers are characterized.
Global epidemiology, genetic environment, risk factors and therapeutic prospects of mcr genes: A current and emerging update

Background Mobile colistin resistance (mcr) genes modify Lipid A molecules of the lipopolysaccharide, changing the overall charge of the outer membrane. Results and discussion Ten mcr genes have been described to date within eleven Enterobacteriaceae species, with Escherichia coli, Klebsiella pneumoniae, and Salmonella species being the most predominant. They are present worldwide in 72 countries, with animal specimens currently having the highest incidence, due to the use of colistin in poultry for promoting growth and treating intestinal infections. The wide dissemination of mcr from food animals to meat, manure, the environment, and wastewater samples has increased the risk of transmission to humans via foodborne and vector-borne routes. The stability and spread of mcr genes were mediated by mobile genetic elements such as the IncHI2 conjugative plasmid, which is associated with multiple mcr genes and other antibiotic resistance genes. The cost of acquiring mcr is reduced by compensatory adaptation mechanisms. MCR proteins are well conserved structurally and via enzymatic action. Thus, therapeutics found effective against MCR-1 should be tested against the remaining MCR proteins. Conclusion The dissemination of mcr genes into the clinical setting, is threatening public health by limiting therapeutics options available. Combination therapies are a promising option for managing and treating colistin-resistant Enterobacteriaceae infections whilst reducing the toxic effects of colistin.
Bardus, Marco and Blake, Holly and Lloyd, Scott and Suzanne Suggs, L. (2014) Reasons for participating and not participating in a e-health workplace physical activity

Purpose. This study aimed to investigate the reasons for participating and not participating in an e-health workplace physical activity (PA) intervention. Methodology. Semi-structured interviews and two focus groups were conducted with a purposive sample of employees who enrolled and participated in the intervention and with those who did not complete enrolment, hence did not participate in it. Data was examined using thematic analysis according to the clusters of “reasons for participation” and for “nonparticipation”. Findings. Reported reasons for participation included a need to be more active, to increase motivation to engage in PA, and to better manage weight. Employees were attracted by the perceived ease of use of the programme and by the promise of receiving reminders. Many felt encouraged to enrol by managers or peers. Reported reasons for non-participation included lack of time, loss of interest towards the programme, or a lack of reminders to complete enrolment. Practical implications. Future e-health workplace behavioural interventions should consider focusing on employees’ needs and motivators to behaviour change, provide regular reminders for participants to complete enrolment and ensure that procedures are completed successfully. Barriers to participation could be identified through formative research with the target population and feasibility studies. Originality/value. This study combines a qualitative analysis of the reasons why some employees decided to enrol in a workplace PA intervention and why some others did not. This study highlights factors to consider when designing, implementing and promoting similar Running head: Reasons for participating in a workplace PA intervention 2 interventions and that could inform strategies to enhance participation in workplace PA
A (cid:2) -Alanine Catabolism Pathway Containing a Highly Promiscuous (cid:3) -Transaminase in the 12-Aminododecanate-Degrading Pseudomonas sp. Strain AAC

We previously isolated the transaminase KES23458 from Pseudomonas sp. strain AAC as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. Here, we report the subsequent characterization of this transaminase. It exhibits activity with a broad substrate range which includes (cid:2) -, (cid:3) -, and (cid:4) -amino acids, as well as (cid:2) , (cid:4) -di-amines and a number of other industrially relevant compounds. It is therefore a prospective candidate for the biosynthesis of a range of polyamide monomers. The crystal structure of KES23458 revealed that the protein forms a dimer containing a large active site pocket and unusual phosphorylated histidine residues. To infer the physiological role of the transaminase, we expressed, puriﬁed, and characterized a dehydrogenase from the same operon, KES23460. Unlike the transaminase, the dehydrogenase was shown to be quite selective, catalyzing the oxidation of malonic acid semialdehyde, formed from (cid:3) -alanine transamination via KES23458. In keeping with previous reports, the dehydrogenase was shown to catalyze both a coenzyme A (CoA)-dependent reaction to form acetyl-CoA and a signiﬁcantly slower CoA-independent reaction to form acetate. These ﬁndings support the original functional assignment of KES23458 as a (cid:3) -alanine transaminase. However, a
MALAYSIAN HOMESTAY OPERATORS AND VISITORS’ SATISFACTION: SERVQUAL ANALYSIS

As one of the tourism products, homestay service in Malaysia documented a successful track record either in terms of the increasing number of homestay operator or the income earned. Malaysia homestays always preferred by the tourist who want to experience the lifestyle and culture of the local community and this rural tourism activity become iconic and high potential tourist attraction. However, some loopholes and weaknesses in the service delivery and the infrastructure is likely to diminish the satisfaction level. Therefore, this study is written to identify the effect of service quality offered by homestay operators on visitors’ satisfaction. 102 respondents who are experienced in staying at the Malaysia homestay were selected to answer the adapted questionnaire. Interval scale was used to record the responses for service quality dimensions and satisfaction parts that range from 1 = Strongly Disagree to 10 = Strongly Agree . The IBM Statistical Package for Social Science (SPSS) Statistics for Windows, Version 24.0 was used to code and analyse the data. This study found a significant positive effect of service quality on visitors’ satisfaction. It is hoped this study contributes to the awareness towards of homestay operators to give good services and upgrading the weaknesses which could affect their business.
THORAXJNL145144 1..3

Our patient was a 62-year-old retired accountant of previous good health. He was not taking any medications and had no family history of neurological disease. He was involved in a minor road traffic accident in which his car was shunted from behind. No immediate injuries were sustained; however, he presented to his general practitioner 4 days later with lumbar spine discomfort. He was prescribed a codeineeparacetamol combination pill and diclofenac. Immediately after taking the first dose of these medications he developed severe dizziness and marked vomiting, which culminated in mild haematemesis, indicative of a MalloryeWeiss tear. Subsequently he discovered that he was dyspnoeic lying flat. He presented to his local emergency department with symptoms including marked orthopnoea, and dyspnoea on water immersion past his costal margin and on bending forward. Physical examination showed gross paradoxical abdominal motion and mild breathlessness when recumbent. Respiratory system, some left basal crackles; cardiovascular system, normal; abdomen, normal; neurology, normal. Arterial blood gas measurements showed a PaO2 of 11.1 kPa and a PaCO2 of 5.1 kPa. A chest radiograph showed an elevated left hemidiaphragm with some atelectasis above it. A CT pulmonary angiogram excluded any diaphragmatic rupture or pulmonary embolus as an underlying cause of orthopnoea. His sniff nasal inspiratory pressure (SNIP) was measured at 22 cm H2O. Detailed testing showed no response to bilateral anterior magnetic phrenic nerve stimulation or to right unilateral phrenic nerve stimulation, with a greatly reduced response to left-sided stimulation (twitch transdiaphragmatic pressure 1.3 cm H2O; normal >8 cm H2O). Pulmonary function tests (performed in the erect position) were consistent with diaphragm paralysis, showing a restrictive pattern with mildly reduced carbon monoxide gas transfer (TLCO 82% predicted) which became supernormal when corrected for alveolar volume (KCO 116% predicted). Forced vital capacity (FVC) was 64% predicted, with a forced expiratory volume in 1 s (FEV1)/FVC ratio of 81% and stable over the course of 7 months follow-up. Clinic FVC testing in the erect position was 2.8 and 1.5 litres when supine (54% reduction). Upright cervical spine MRI revealed minor degenerative changes at C5/6 only. Nerve conduction studies (arms and legs) and needle electromyograph (EMG) examination of the upper limbs were normal.
Decreased Expression of miR-216a Contributes to Non-small Cell Lung Cancer Progression

Purpose: The aim of the present study is to investigate the role and mechanism of miR-216a in non-small cell lung cancer (NSCLC). Experimental Design: The expression of miR-216a in NSCLC cell lines and from NSCLC patient specimens was measured by real time qRT-PCR. The correlation between gene expression and patient survival was analyzed using Kaplan-Meier methods. The effects of miR-216a on NSCLC cell growth and metastasis were examined both in vitro and in vivo by overexpressing or inhibiting miR-216a. Finally, the effect of miR-216a on chemoresistance was investigated by MTT assay and flow cytometry. Results: miR-216a expression was downregulated in specimens from NSCLC patients compared to corresponding nontumor lung tissues. Clinical data indicate that decreased miR-216a expression is inversely correlated with cancer stage, metastasis and poor survival in NSCLC patients. Our data also show that overexpression of miR-216a suppresses NSCLC cell growth, metastasis and enhances cisplatin-induced cell growth inhibition and apoptosis. In contrast, inhibition of miR-216a stimulates NSCLC cell growth, metastasis and suppresses cisplatin-induced cell growth inhibition and apoptosis. Furthermore, we demonstrate that miR-216a exerts its role by directly targeting eIF4B and ZEB1. Conclusion: Our findings suggest that miR-216a is a cancer suppressor miRNA and that overexpression of miR-216a is a novel NSCLC treatment strategy. In addition, our clinical data indicate that miR-216a may be a useful biomarker for predicting NSCLC progression.
TACG_A_262866 241..254

1Department of Hematology and Medical Oncology, Boston University Medical Center, Boston, MA, USA; 2Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA Abstract: Multiple myeloma (MM) is a heterogeneous disease featured by clonal plasma cell proliferation and genomic instability. The advent of next-generation sequencing allowed unraveling the complex genomic landscape of the disease. Several recurrent genomic aberrations including immunoglobulin genes translocations, copy number abnormalities, complex chromosomal events, transcriptomic and epigenomic deregulation, and mutations define various molecular subgroups with distinct outcomes. In this review, we describe the recurrent genomic events identified in MM impacting patients’ outcome and survival. These genomic aberrations constitute new markers that could be incorporated into a prognostication model to eventually guide therapy at every stage of the disease.
Does environmental peacemaking between states work? Insights on cooperative environmental agreements and reconciliation in international rivalries

The literature on environmental peacemaking argues that cooperation in the face of shared environmental challenges can facilitate further cooperation, trust building, and eventually peace between states in conflict. Empirical research on environmental peacemaking, predominantly conducted in the form of single case studies, has so far been inconclusive. This article uses a cross-case, multimethod research design to test the environmental peacemaking proposition. More specifically, it argues that the conclusion of a cooperative environmental agreement can have a positive impact on reconciliation between rival states. Based on a new dataset on international rivalry termination, transboundary protected areas, and international freshwater agreements, this article first conducts a statistical analysis and a qualitative comparative analysis (QCA). The results are then verified and refined by six case studies. Triangulation of findings from the three studies suggests that the conclusion of a cooperative environmental agreement facilitates reconciliation in international rivalries. But this effect is contingent on a number of scope conditions, such as high environmental attention, internal political stability, wider patterns or traditions of environmental cooperation, and already ongoing processes of reconciliation. Still, the findings imply that environmental challenges do not only affect peace and security in a negative way. Addressing them jointly also opens opportunities for peacemaking and peacebuilding between states.