Miracle-dz/UniFAR-FTUs huggingface dataset:
Associations between parent-child relationship, self-esteem, and resilience with life satisfaction and mental wellbeing of adolescents

Purpose Mental wellbeing and life satisfaction play an important role in the development of adolescents, yet factors potentially influencing these states have not been sufficiently studied, especially in Vietnam. This study aims to fill the research gaps by exploring the associations of the parent-child relationship, self-esteem, and resilience on the mental wellbeing and satisfaction with life of adolescents. Methods A cross-sectional study was conducted from June to July 2020 on 1,023 adolescents from 10 to 18 years old living in Vietnam. To assess the satisfaction with life as well as the mental wellbeing of participants, this study used the Satisfaction with life and The World Health Organization-Five Wellbeing Index scale. Results More than 70% of participants reported having conflicts with their parents (74.6% of those conflicted with their father ad 73.9% of those conflicted with their mother), while 26.3% stated dissatisfaction with life. The mean score of mental wellbeing was 61.5 (SD = 23.0). Higher academic performance, self-esteem, resilience, encounter loneliness and isolation within own family, and having support and sharing from family members had a positive effect on life satisfaction and mental wellbeing. Female participants had higher satisfaction with life score (Coef = 0.77; 95%CI = 0.10; 1.44) but they had a lower mental wellbeing score (Coef = −6.00; 95%CI = −8.57; −3.44) than male participants. High school students had lower both satisfaction with life and mental wellbeing scores than secondary students. Conclusion The results highlight the importance of being aware of the influence that expectations (of higher grades) and bias (toward male children) imposed by parents, teachers, and society on the mental wellbeing of youths, especially in Asian cultures. Strengthening the family bond and encouraging young people to share their feeling is also crucial to enhancing the mental health condition of adolescents.
dsRNase1 contribution to dsRNA degradation activity in the Sf9 cells conditioned medium

RNA interference (RNAi) is inefficient in lepidopteran insects, including Spodoptera frugiperda. RNase activity in the lumen and hemocoel is known to contribute to low RNAi efficiency in these insects. Conditioned medium from Sf9 cells developed from ovaries of S. frugiperda shows high dsRNA degradation activity. But the enzymes responsible for this activity have not been identified. The nuclease genes that are highly expressed in Sf9 cells, REase, RNaseT2, and dsRNase1, were identified. Knockdown of dsRNase1 in Sf9 cells resulted in a reduction of dsRNA degradation activity in the Sf9 cells conditioned medium. Knockdown of dsRNase1 also increased RNAi efficiency in Sf9 cells. The results from these studies identified a major player in dsRNA degradation activity in the Sf9 cells conditioned medium. We also describe an efficient system that can be used to identify other genes responsible for dsRNA degradation and RNAi efficiency in Sf9 cells.
The influence of physical exercise on college students’ life satisfaction: The chain mediating role of self-control and psychological distress

Objective To explore the relationship between physical exercise and life satisfaction among college students and test the dual mediating role of self-control and psychological distress between them. Methods A sample of 526 Chinese college students completed questionnaires regarding physical exercise, life satisfaction, self-control and psychological distress, of which 38.4% were boys. Results Path analyzes indicated that physical exercise was positively correlated with life satisfaction, and this link could be mediated by self-control and psychological distress. Conclusion The present study identifies the potential underlying mechanism by which physical exercise is associated with the life satisfaction of college students, which has important implications for theory and prevention.
Progress and prospect of the time-varying gravity in earthquake prediction in the Chinese Mainland

This paper mainly introduces the application and progress of the time-varying gravity in earthquake research in the Chinese Mainland. Since the Xingtai earthquake in 1966, China has begun mobile gravity monitoring, trying to explore the relationship between gravity changes and seismic activities. The gravity changes before and after the Haicheng M S7.3 earthquake in 1975 and the Tangshan M S7.8 earthquake in 1976 were observed. In 1981, a high-precision metal spring gravimeter was introduced to carry out high-precision mobile gravity observation in the key earthquake monitoring areas in western Yunnan. The gravity anomaly changes near the epicenters of the Lijiang M S7.0 earthquakes in 1996 were observed. In 1998, a high-precision absolute gravity survey was introduced to carry out the overall scale gravity field monitoring in the Chinese Mainland, and the large-scale gravity change information before Wenchuan M S8.0 and Yutian M S7.3 earthquakes in 2008 was obtained, and the effective prediction opinions were given. After the Wenchuan M S8.0 earthquake in 2008, the integration of the national network and the regional network accelerated, forming the whole gravity observation network in the Chinese Mainland, which made a relatively successful medium-term prediction for a series of earthquakes with M S6.0 or above (such as Lushan M S7.0, Menyuan M S6.4, and Jiuzhaigou M S7.0) in recent years and played an important role in the study of the earthquake mechanism and earthquake prediction level in China. Finally, the existing problems in time-varying gravity monitoring in China are pointed out, and the prospect of earthquake research using time-varying gravity monitoring data is put forward.
Combining ability and testcross performance of multi-nutrient maize under stress and non-stress environments

While significant progress has been made by several international breeding institutions in improving maize nutritional quality, stacking of nutritional traits like zinc (Zn), quality protein, and provitamin A has not received much attention. In this study, 11 newly introduced Zn-enhanced inbred lines were inter-mated with seven testers from normal, provitamin A and quality protein maize (QPM) nutritional backgrounds in order to estimate the general combining ability (GCA) and specific combining ability (SCA) for grain yield (GY) and secondary traits under stress conditions [(combined heat and drought stress (HMDS) and managed low nitrogen (LN)] and non-stress conditions [(summer rainfed; OPT) and well-watered (irrigated winter; WW)] in Zimbabwe. Lines L6 and L7 had positive GCA effects for GY and secondary traits under OPT and LN conditions, and L8 and L9 were good general combiners for GY under HMDS conditions. Superior hybrids with high GY and desirable secondary traits were identified as L10/T7 and L9/T7 (Zn x normal), L2/T4, L4/T4, L3/T5 (Zn x provitamin A), and L8/T6 and L11/T3 (Zn x QPM), suggesting the possibility of developing Zn-enhanced hybrids with high yield potential using different nutritional backgrounds. Both additive and dominance gene effects were important in controlling most of the measured traits. This suggests that selecting for desirable traits during inbred line development followed by hybridization and testing of specific crosses under different management conditions could optimize the breeding strategy for stacked nutritionally-enhanced maize genotypes.
A novel approach for large-scale manufacturing of small extracellular vesicles from bone marrow-derived mesenchymal stromal cells using a hollow fiber bioreactor

Mesenchymal stromal cells (MSCs) are promising therapeutic candidates in a variety of diseases due to having immunomodulatory and pro-regenerative properties. In recent years, MSC-derived small extracellular vesicles (sEVs) have attracted increasing interest as a possible alternative to conventional cell therapy. However, translational processes of sEVs for clinical applications are still impeded by inconsistencies regarding isolation procedures and culture conditions. We systematically compared different methods for sEV isolation from conditioned media of ex vivo expanded bone marrow-derived MSCs and demonstrated considerable variability of quantity, purity, and characteristics of sEV preparations obtained by these methods. The combination of cross flow filtration with ultracentrifugation for sEV isolation resulted in sEVs with similar properties as compared to isolation by differential centrifugation combined with ultracentrifugation, the latter is still considered as gold standard for sEV isolation. In contrast, sEV isolation by a combination of precipitation with polyethylene glycol and ultracentrifugation as well as cross flow filtration and size exclusion chromatography resulted in sEVs with different characteristics, as shown by surface antigen expression patterns. The MSC culture requires a growth-promoting supplement, such as platelet lysate, which contains sEVs itself. We demonstrated that MSC culture with EV-depleted platelet lysate does not alter MSC characteristics, and conditioned media of such MSC cultures provide sEV preparations enriched for MSC-derived sEVs. The results from the systematic stepwise evaluation of various aspects were combined with culture of MSCs in a hollow fiber bioreactor. This resulted in a strategy using cross flow filtration with subsequent ultracentrifugation for sEV isolation. In conclusion, this workflow provides a semi-automated, efficient, large-scale-applicable, and good manufacturing practice (GMP)-grade approach for the generation of sEVs for clinical use. The use of EV-depleted platelet lysate is an option to further increase the purity of MSC-derived sEVs.
Uveal melanoma incidence trends in Canada: 1992–2010 vs. 2011–2017

Background/aims Uveal melanoma is the most common type of non-cutaneous melanoma and the most common ocular malignancy in the adult population, especially affecting Caucasians (98% of cases). Despite its low incidence rate, we have noted increasing incidence trends in recent years. Methods We analyzed uveal melanoma incidence data using the Canadian Cancer Registry (CCR) for 2011–2017 years. The data was examined using the International Classification of Diseases for Oncology, Third Edition, codes for all uveal melanoma subtypes. The data for 2011–2017 was then compared to previously published work by our research group for uveal melanoma incidence in Canada between 1992 and 2010 using the same methodology. Results Between 2011 and 2017, 1,215 patients were diagnosed with uveal melanoma, 49% of whom were females. The percentage distribution of uveal melanoma between the sexes was similar between 1992–2010 and 2011–2017, whereby of the 2,215 diagnoses of uveal melanoma in 1992–2010, 47.9% were females. The change in the incidence rate for this cancer has doubled between 1992–2010 and 2011–2017, from 0.074 to 0.15 cases per million individuals per year. Our study documents that the Canadian 2011–2017 age-standardized incidence rate (ASIR) for uveal melanoma against the World Health Organization (WHO) 2000–2025 world population standard was 5.09 cases per million individuals per year (95% confidence interval, 4.73–5.44), as compared with the 1992–2010 rate of 3.34 cases per million individuals per year (95% confidence interval, CI 3.20 to 3.47). Conclusion This work demonstrates an ongoing, steady increase in uveal melanoma incidence in Canada in recent years.
Gravitational lensing frequencies: galaxy cross-sections and selection effects

Four issues - (1) the best currently available data on the galaxy velocity-dispersion distribution, (2) the effects of finite core radii potential ellipticity on lensing cross sections, (3) the predicted distribution of lens image separations compared to observational angular resolutions, and (4) the preferential inclusion of lens systems in flux limited samples - are considered in order to facilitate more realistic predictions of multiple image galaxy-quasar lensing frequencies. It is found that (1) the SIS lensing parameter F equals 0.047 +/-0.019 with almost 90 percent contributed by E and S0 galaxies, (2) observed E and S0 core radii are remarkably small, yielding a factor of less than about 2 reduction in total lensing cross sections, (3) 50 percent of galaxy-quasar lenses have image separations greater than about 1.3 arcsec, and (4) amplification bias factors are large and must be carefully taken into account. It is concluded that flat universe models excessively dominated by the cosmological constant are not favored by the small observed galaxy-quasar lensing rate.
Hereditable variants of classical protein tyrosine phosphatase genes: Will they prove innocent or guilty?

Protein tyrosine phosphatases, together with protein tyrosine kinases, control many molecular signaling steps that control life at cellular and organismal levels. Impairing alterations in the genes encoding the involved proteins is expected to profoundly affect the quality of life—if compatible with life at all. Here, we review the current knowledge on the effects of germline variants that have been reported for genes encoding a subset of the protein tyrosine phosphatase superfamily; that of the thirty seven classical members. The conclusion must be that the newest genome research tools produced an avalanche of data that suggest ‘guilt by association’ for individual genes to specific disorders. Future research should face the challenge to investigate these accusations thoroughly and convincingly, to reach a mature genotype-phenotype map for this intriguing protein family.
Role of plaque inflammation in symptomatic carotid stenosis

Objective Prior studies have shown that plaque inflammation on FDG-PET and the symptomatic carotid atheroma inflammation lumen-stenosis (SCAIL) score were associated with recurrent ischemic events, but the findings have thus far not been widely validated. Therefore, we aimed to validate the findings of prior studies. Methods A single-center prospective cohort study that recruited patients with (1) recent TIA or ischemic stroke within the past 30 days, (2) ipsilateral carotid artery stenosis of ≥50%, and (3) were not considered for early carotid revascularization. The (1) maximum standardized uptake value (SUVmax) of the symptomatic carotid plaque, (2) the SCAIL score, and (3) stenosis severity of the symptomatic carotid artery were measured for all patients. The outcomes were (1) a 90-day ipsilateral ischemic stroke and (2) a 90-day ipsilateral symptomatic TIA or major adverse cardiovascular event (MACE). Results Among the 131 patients included in the study, the commonest cardiovascular risk factor was hypertension (95 patients, 72.5%), followed by diabetes mellitus (77 patients, 58.8%) and being a current smoker (64 patients, 48.9%). The median (IQR) duration between the index cerebral ischemic event and recruitment to the study was 1 (0, 2.5) days. The median (IQR) duration between the index cerebral ischemic event and FDG-PET was 5 (4, 7) days. A total of 14 (10.7%) patients had a 90-day stroke, and 41 (31.3%) patients had a 90-day TIA or MACE. On comparison of the predictive performances of the SCAIL score and SUVmax, SUVmax was found to be superior to the SCAIL score for predicting both 90-day ipsilateral ischemic stroke (AUC: SCAIL = 0.79, SUVmax = 0.92; p < 0.001; 95% CI = 0.072, 0.229) and 90-day TIA or MACE (AUC: SCAIL = 0.76, SUVmax = 0.84; p = 0.009; 95% CI = 0.020, 0.143). Conclusion Plaque inflammation as quantified on FDG-PET may serve as a reliable biomarker for risk stratification among patients with ECAD and recent TIA or ischemic stroke. Future studies should evaluate whether patients with significant plaque inflammation as quantified on FDG-PET benefit from carotid revascularization and/or anti-inflammatory therapy.
Evaluation of the efficacy of the antimicrobial peptide HJH-3 in chickens infected with Salmonella Pullorum

As a promising substitute for antibiotics, increasing attention has been given to the clinical application of antimicrobial peptides (AMPs). In this study, the mode of action of the HJH-3 against Salmonella Pullorum was investigated. The structure and properties of HJH-3 were examined in silico, and minimum inhibitory concentrations (MICs) were determined to evaluate its antimicrobial spectrum. The time-kill kinetics of HJH-3 was determined. The hemolytic activity of HJH-3 was determined by measuring the hemoglobin ultraviolet absorption value, and the cytotoxicity was determined using a CCK-8 kit. The protective effect of HJH-3 on chickens infected with S. Pullorum was evaluated in vivo. The results demonstrated that HJH-3 exhibited strong antibacterial activity against Gram-negative pathogens at MIC values of 1.5625–25 μg/mL and against Gram-positive pathogens at MIC values of 25–50 μg/mL. HJH-3 also showed activity against the Candida albicans (100 μg/mL) and Bacillus subtilis (6.25–12.5 μg/mL). HJH-3 at 100 μg/mL completely killed S. Pullorum after co-incubation for 6 h. Likewise, the hemolysis rate of CRBCs treated with 100 μg/mL HJH-3 (7.31%) was lower than that of CRBCs treated with 100 μg/mL pexiganan (40.43%). Although the hemolysis rate of CRBCs treated with 400 μg/mL HJH-3 was increased to 13.37%, it was much lower than that of 400 μg/mL pexiganan (57.27%). In regards to cytotoxicity, HJH-3 had almost no-effect on the CEF proliferation, pexiganan decreased CEFs proliferation from 56.93 to 31.00% when increasing the concentration from 50 to 200 μg/mL. In a chicken infection model, the results showed that the antibiotic prevention and HJH-3 prevention groups exhibited the best treatment effect, with the chickens being protected from the lethal dose of S. Pullorum, a decreased number of bacteria in the blood and spleen, and less pathological changes in intestinal segments. The prevention of infection by HJH-3 was similar to that by Ampicillin; the effect of treatment after infection was lower than that of treatment before infection, and the survival rate of infected chicks treated with HJH-3 was 70%, which was still higher than that of the infected chickens. These results suggest that HJH-3 has good clinical application potential and can be used as a substitute for antibiotics for the prevention and treatment of S. Pullorum infection.
Osteoimmune regulation underlies oral implant osseointegration and its perturbation

In the field of biomaterials, an endosseous implant is now recognized as an osteoimmunomodulatory but not bioinert biomaterial. Scientific advances in bone cell biology and in immunology have revealed a close relationship between the bone and immune systems resulting in a field of science called osteoimmunology. These discoveries have allowed for a novel interpretation of osseointegration as representing an osteoimmune reaction rather than a classic bone healing response, in which the activation state of macrophages ((M1–M2 polarization) appears to play a critical role. Through this viewpoint, the immune system is responsible for isolating the implant biomaterial foreign body by forming bone around the oral implant effectively shielding off the implant from the host bone system, i.e. osseointegration becomes a continuous and dynamic host defense reaction. At the same time, this has led to the proposal of a new model of osseointegration, the foreign body equilibrium (FBE). In addition, as an oral wound, the soft tissues are involved with all their innate immune characteristics. When implant integration is viewed as an osteoimmune reaction, this has implications for how marginal bone is regulated. For example, while bacteria are constitutive components of the soft tissue sulcus, if the inflammatory front and immune reaction is at some distance from the marginal bone, an equilibrium is established. If however, this inflammation approaches the marginal bone, an immune osteoclastic reaction occurs and marginal bone is removed. A number of clinical scenarios can be envisioned whereby the osteoimmune equilibrium is disturbed and marginal bone loss occurs, such as complications of aseptic nature and the synergistic activation of pro-inflammatory pathways (implant/wear debris, DAMPs, and PAMPs). Understanding that an implant is a foreign body and that the host reacts osteoimmunologically to shield off the implant allows for a distinction to be drawn between osteoimmunological conditions and peri-implant bone loss. This review will examine dental implant placement as an osteoimmune reaction and its implications for marginal bone loss.
Effects of bariatric surgery on testosterone level and sexual function in men with obesity: A retrospective study

Introduction Bariatric surgeries induce well-documented weight loss and resolve obesity comorbidities. Sexual function is one of the aspects of life quality and may benefit from surgery. Few studies have revealed the impact of bariatric surgeries on sexual function in Chinese men with obesity. Methods This is a retrospective cohort study of patients undergoing bariatric surgery [laparoscopic sleeve gastrectomy (LSG) or laparoscopic Roux-en-Y gastric bypass (LRYGB)]. Data were collected between September 2017 and February 2022. The International Index of Erectile Function (IIEF) questionnaire was used to evaluate erectile function, intercourse satisfaction, orgasmic function, sexual desire, and overall satisfaction. Sex hormones and other blood tests were evaluated before and at least 1 year after the surgery. Results Fifty-nine Chinese male patients completed the IIEF questionnaire. The multivariate logistic regression analysis revealed that body mass index (BMI) was the single independent risk factor of the severity of erectile dysfunction (ED). Preoperative testosterone levels had negative correlations with BMI and waist circumference. Thirty-seven patients completed the postoperative questionnaire with a mean follow-up of 23.2 months. Conclusion BMI and waist circumference were negatively correlated with testosterone levels. BMI was an independent risk factor for the severity of ED. LSG and LRYGB led to positive and sustained improvement in sexual function of men with obesity. The two procedures had a comparable effect, more subjects being needed. Sex hormone levels also could be reversible. However, more weight loss did not predict a positive change in sexual function. A greater BMI loss might predict a greater increase in testosterone.
The 22 May 2007 B-class flare: new insights from Hinode observations

We present multi-wavelength observations of a small B-class flare which occurred on the Sun on 2007 May 22. The observations include data from Hinode, GOES, TRACE and the Nobeyama Radioheliograph. We obtained spatially and spectrally-resolved information from the Hinode EUV Imaging Spectrometer (EIS) during this event. The temporal and temperature coverage of the EIS observations provides new insights into our understanding of chromospheric evaporation and cooling. The flare showed many "typical" features, such as brightenings in the ribbons, hot (10 MK) loop emission and subsequent cooling. We also observed a new feature, strong (up to 170 km s -1 ) blue-shifted emission in lines formed around 2-3 MK, located at the footpoints of the 10 MK coronal emission and within the ribbons. Electron densities at 2 MK in the kernels are high, of the order of 10" cm -3 , suggesting a very narrow layer where the chromospheric evaporation occurs. We have run a non-equilibrium hydrodynamic numerical simulation using the HYDRAD code to study the cooling of the 10 MK plasma, finding good agreement between the predicted and observed temperatures, densities and ion populations. Line blending for some potentially useful diagnostic lines for flares, which are observed with Hinode/EIS, is also discussed.