Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular interaction of human neutrophil NADPH oxidase proteins

Human neutrophils respond to a variety o f soluble and particulate factors that recruit these cells to areas o f inflammation and infection. At these sites, neutrophils phagocytose foreign particles and destroy them using a combination o f microbicidal mechanisms including the production o f microbicidal oxidants such as superoxide anion (O2"). The generation o f O2 by neutrophils requires the assembly o f a membrane-bound complex known as the NADPH oxidase, which is composed of a plasma membrane flavocytochrome b, a membrane-associated low molecular weight GTP-binding protein (Rap I A), and four cytosolic proteins ($41-phox, ySl-phox, p40-phox, and Rac2). During activation, cytosolic proteins p47-phox, ^61-phox, Rac2, and possibly p40-phox translocate to the plasma membrane and associate with flavocytochrome b to form the active complex. The activation and assembly o f the NADPH oxidase is regulated by signaling events and by molecular interaction between its components. These processes are not well characterized, and an understanding o f how these protein components interact is essential to understanding its regulation. Several biochemical and molecular biological approaches were used in the current studies to define and subsequently confirm novel interactions occurring between flavocytochrome b and p47-p/zox and between p47-phox and ^61-phox. Two novel regions o f p47-p/zox:flavocytochrome b interaction were mapped to gp91-p/zox residues 86-93 and 450-457. The mapping also confirmed previously reported binding domains on gp91-p/zox (554-564) and putative SH3 domain binding sites on ̂ 22-phox (158-162 and 177-183). Using a complimentary approach, p47phox residues 323-342 were identified as a flavocytochrome b binding site. Interestingly, a novel region in ^Al-phox encompassing residues 323-332 (which is within the flavocytochrome b binding site) and a previously identified SH3-binding domain encompassing pAl-phox residues 361-370 were identified as ^61-phox binding sites. P67phox and flavocytochrome b were found to compete for the binding ofp47-phox after activation, suggesting that both p67-p/zox and flavocytochrome b utilize a common binding site in -pAl-phox, presumably at distinct stages during the activation process. This ^Al-phox region appears to play a key role in regulating NADPH oxidase assembly. The identification o f these novel interactions has provided a better understanding o f the mechanism o f NADPH oxidase assembly and activation. I CHAPTER
Behavior of soybean lines in the Triângulo Mineiro and Goiatuba-GO.

Este trabalho avaliou a produtividade de graos de 24 linhagens de soja, com ciclo de maturacao semitardio e tardio, provenientes de cruzamentos realizados no Programa de Melhoramento de Soja da Universidade Federal de Uberlândia. Foram utilizadas quatro testemunhas ou cultivares comerciais adaptadas a regiao e de boa produtividade, sendo Emgopa 313 RCH, Garantia, DM 339 e Suprema. A magnitude da interacao genotipo por ambiente (G x E), adaptabilidade e estabilidade, utilizou-se o metodo de Regressao Linear Simples de Eberhart e Russell (1966), Regressao Bissegmentada de Cruz; Torres; Vencovsky (1989) e o Metodo de Lin e Binns (1988), em testes regionais, foram cinco locais: Goiatuba-GO, Araguari-MG, Sao Gotardo-MG, Uberlândia-MG e Uberaba-MG. O delineamento foi o de blocos ao acaso, com 3 repeticoes, sendo as parcelas constituidas de 4 fileiras de 5 metros de comprimento, espacadas entre si com 0,45 m e parcela util as duas fileiras centrais. Apos a colheita obteve-se peso dos graos da area util da parcela, e foram submetidos a uma analise de variância individual por local, e conjunta, considerando-se os cinco locais, utilizando-se o procedimento GLM do programa – SAS, General Linear Models Procedure (STATISTICAL ANALYSIS SYSTEM – SAS, 1997). As linhagens (UFU99-0342, UFU99-091126, UFU99-11704, UFU99-10922, UFU99-041121, UFU99-031105 e UFU99-101127) reuniram boa produtividade de graos, adaptabilidade e estabilidade nos locais de teste.
Pine processionary moth (Thaumetopoea pityocampa) and great tit (Parus major) in Portugal

In the present work, we studied a common outbreaking Lepidoptera species in Portuguese pine stands – Thaumetopoea pityocampa (Den. & Schiff.) and one of its potential predators – Parus major (L.). The population dynamics of the immature stages of the Lepidoptera was studied in several types of Pinus pinaster (Aiton) plantations in three different areas: Setúbal Peninsula, Abrantes and National Pine Forest of Leiria. Location and plantation structure was the most important factors determining population density of T. pityocampa. Setubal and Abrantes was highly susceptible to attacks by the Lepidoptera, whereas Leiria had lower densities. Young and homogeneous pine stands was more susceptible to attacks than older and more heterogeneous pines stands. However, a desynchronized population of T. pityocampa, in which the larvae develops during summer instead of during winter, reached high densities also in Leiria. The impact of several mortality factors and climatic conditions on the immature stages of the insect (eggs and larvae), in normal and desynchronized populations are discussed, as well as possible evolutionary implications of the sudden appearance of the new version of T. pityocampa. The break of the pupa diapause and adult emergence times the annual life cycle of this insect. Adults from the desynchronized population emerged earlier than adults from the normal population, which in turn determined the change in the larvae development period. Different factors, potentially affecting the timing of adult emergence in both normal and abnormal populations are also discussed. To study P. Major, nest-boxes were placed in the areas of Setúbal and Leiria and they were monitored during three seasons. The nest-boxes increased the density of breeding and wintering birds in the studied pine plantations, indicating that a lack of natural holes are in fact a limiting factor for this populations. The earliest breeding start for this species was recorded in my study area, indicating that Portuguese coastal pines provide good breeding conditions earlier than in other areas of Europe and North Africa. This leads to an overlap between the end of the larvae stage of T. pityocampa and the beginning of the breeding season of P. major. Key-words: Thaumetopoea pityocampa, Parus major, Pinus pinaster, population dynamics, Portugal.
A joint confidence region for an overall ranking of populations

National statistical agencies lack statistical methodology to express uncertainty in their released estimated overall rankings. For example, the US Census Bureau produced an ‘explicit’ ranking of the states based on observed sample estimates during 2011 of mean travel time to work. Current literature provides measures of uncertainty in estimated individual ranks, but not a direct measure of uncertainty for the estimated overall ranking. We construct and visualize a joint confidence region for the true unknown overall ranking that provides a measure of uncertainty in the estimated overall ranking.
Antibody-mediated inhibition of proteases of African trypanosomes.

The protozoan parasites Trypanosoma congolense and T. vivax cause trypanosomosis in cattle. The major lysosomal cysteine proteinase of T. congolense, congopain, may contribute to pathogenesis of the disease, and antibody-mediated inhibition of this enzyme may contribute to mechanisms of trypanotolerance. Oligopeptidase B, a trypanosomal serine peptidase, is also a potential virulence factor in African trypanosomes because it is released into the host circulation by dead or dying parasites, where it retains catalytic activity due to the enzyme's insensitivity to serum protease inhibitors. The vaccine potential of the catalytic domain of congopain, C2, and oligopeptidase B complexed with 0'2-macroglobulin (0'2M) was evaluated by producing antibodies in rabbits. Inhibition of congopain and oligopeptidase B activity by these antibodies was assessed. The oligopeptidase B open reading frame from T. congolense and T. vivax was cloned and expressed in Escherichia coli, from which active recombinant enzymes were purified. These recombinant enzymes exhibited trypsin-like specificity for peptide substrates, cleaving on the carboxy side of basic amino acid residues such as arginine and lysine. Enzymes were found to be optimally active between pH 8 and 10, optimally stable at pH 6, and showed activation by reducing agents and sensitivity to ionic strength. The enzymes showed typical oligopeptidase B-like inhibitor profiles, except that they were not inhibited by thiol sensitive inhibitors such as iodoacetamide and Nethylmaleimide. High yields of bovine and rabbit 0'2M were isolated by a three-step procedure of fractionation by PEG 6000, and zinc chelate and Sephacryl S-300 HR chromatography. Congopain, its catalytic domain C2, papain and cathepsin L all cleaved the bait region of bovine 0'2M and became trapped inside the 0'2M molecule, where their activity against large molecular weight substrates was inhibited. C2 could thus be complexed with 0'2M directly or used to form C2-0'2M-oligopeptidaseB complexes for immunisation purposes.
Determinants of Interest Margin in Pakistan: A Panel Data Analysis

This paper explores the determinants of the net interest margin (NIM) using unbalanced panel data from 2003 to 2017. This paper’s objective is achieved by using a two-step system generalised method of moment (GMM) for estimation. Three different models are to account for two alternative measures of market competition. The empirical results of these models indicate that operating cost, profit tax, interest rate risk, Lerner index, national savings, money supply and the T-bill rate have significant positive associations with the NIM. Meanwhile, operational size, credit risk and the inflation rate negatively affect NIM. Furthermore, operating cost, taxation and the money supply are the main factors that influence the NIM. The present analysis provides a comprehensive view of the Lerner index, which is a better measure for capturing the degree of market power than the concentration measure (HHI). Managerial efficiency and risk aversion are bank-level factors that do not significantly determine NIM. Operating costs could be decreased by adopting new banking technologies. Therefore, higher competition, lower operating costs and restricted uncertain conditions in a macro environment, particularly in the money market, could reduce the NIM.
Forensic Verification of Health Data From Wearable Devices Using Anonymous Witnesses

The use of wearable devices, such as smartwatches, glasses, clothes, and fitness bracelets is increasing at an ever-growing pace. Major corporations and insurance companies have started mandating their use for their employees and clients. Data from such devices have begun to feature in settlement claims and as evidence in courts as well, requiring it to be irrefutable and tamper-proof. Lack of protection for personal data as well as the contextual information such as location tracking and its use by law enforcement agencies is raising serious privacy concerns among the general public and civil liberty advocates. In this article, we propose a novel scheme to secure the wearable sensor’s communication through its crowdsourced logging by neighboring wearable and smart devices called witnesses preserving the contextual information (such as time and location) as well. To ensure witness privacy, gateway and witness devices use the reciprocity property of wireless medium between them to generate pairs of closely matching link signatures, which not only provide the proof of presence for the witnesses in the vicinity but also act as their time-varying pseudonyms. We demonstrate the feasibility and efficacy of our scheme through the prototype implementation using real wireless devices, and via simulation and experimental results.
Significant association of a single nucleotide polymorphism in the upstream region of FGFR1OP2/wit3.0 gene with residual ridge resorption of mandible in Saudis

: Residual ridge resorption (RRR) is the decrease in the jaw structure that follows tooth extraction. It is a multifactorial disorder, but reports on the associated genetic factors are scarce, particularly amongst the Saudis. This study aimed to investigate the role of single nucleotide polymorphisms (SNPs) in fibroblast growth factor receptor 1 oncogene partner 2 (FGFR1OP2) in RRR development in Saudis. The study included 192 individuals (RRR = 96; controls = 96) attending outpatient clinics at the College of Dentistry, King Saud University. Demographic and clinical data were collected, the digital panoramic dental radiograph was obtained, and mandibular residual ridge height was measured. DNA was extracted from saliva and genotyping was conducted on “Sequenom MassARRAY iPLEX”. Genotype and allele frequencies of three SNPs were calculated and compared. The age at first diagnosis and bone height were compared in the three genotypes of each SNP. The age of the patients, age at first edentulism, and bone height ranged 21-80 years, 12-70 years, and 13-34.6 mm, respectively. All three genotypes of the studied SNPs (rs2279351, rs78054962 and rs2306852) were identified. SNP rs2279351 associated significantly with RRR, and the mutant C allele was highly predisposing. No association was observed for the other two SNPs. The genotypes of all SNPs had an influence on age at first edentulism and bone height, but the results were not statistically different. Since FGFR1OP2 plays a role in the process of rapid wound healing in the oral cavity, it may be playing a role in the development of RRR by influencing the rate of resorption of the jawbone. SNP rs2279351 may alter its expression and hence RRR development. This study is limited due to small a sample size, and further large-scale studies are required to confirm this association and to consider rs2279351 as a possible marker of RRR development.
Cross-regional modelling of fire occurrence in the Alps and the Mediterranean Basin

In recent decades, changes in fire activity have been observed in Europe. Fires can have large consequences for the provisioning of ecosystem services and for human well-being. Therefore, understanding the drivers of fire occurrence and improving the predictive capability of fire occurrence models is of utmost importance. So far, most studies have focused on individual regions with rather low spatial resolution, and have lacked the ability to apply the models in different regions. Here, a species distribution modelling approach (Maxent) was used to model fire occurrence in four regions across the Mediterranean Basin and the Alps using several environmental variables at two spatial resolutions. Additionally, a cross-regional model was developed and spatial transferability tested. Most models showed good performance, with fine resolution models always featuring somewhat higher performance than coarse resolution models. When transferred across regions, the performance of regional models was good only under similar environmental conditions. The cross-regional model showed a higher performance than the regional models in the transfer tests. The results suggest that a cross-regional approach is most robust when aiming to use fire occurrence models at the regional scale but beyond current environmental conditions, for example in scenario analyses of the impacts of climate change.
Dormancy and pregerminative treatments in Leucaena spp. seeds harvested on acid soil.

A trial was conducted with seeds of Leucaena spp accessions harvested on acid soil and stored under ambient conditions, in order to determine the period and intensity of dormancy and its breaking. For that purpose a simple classification design and four replications were used, with the following treatments: 1) untreated seed (control); 2) coat cut; and 3) water at 80oC for 2 minutes. In each one of the accessions there were significant differences among the applied treatments and the highest germination values were found with the seed coat cut. All the accessions showed dormant state (between 10 and 98%), which was observed in the germination of the control and the viability with the seed coat cut. The highest dormancy values in the seeds harvested in 2006 were detected in L. macrophylla CIAT 17233, L. macrophylla CIAT 17231, L. diversifolia CIAT 17270 and L. leucocephala IH-1140, which hardness was 98,0; 97,0; 80,0 and 74,7%, respectively; in the seeds harvested in 2003 and 2004, the hardness percentages were 89,0; 69,0 and 60,0% for L. lanceolata CIAT 17252, L. macrophylla CIAT 17238 and L. leucocephala cv. K-67, respectively. The seeds of the accessions were concluded to show postharvest dormancy, which varied between 10 and 98%. In addition, thermal scarification caused dormancy breaking, for which the application of this procedure before planting to Leucaena spp seeds harvested on acid soil in Cascajal, is recommended.
Effect of low levels of dietary crude protein with constant metabolizable energy on nitrogen excretion, litter composition and blood parameters of broilers.

A study was conducted to determine the effect of lowering dietary crude protein (CP) with optimum limiting amino acids (AA) levels on nitrogen (N) excretion, litter composition and blood parameters of broilers from 1 to 35 days of age. Four isocaloric broiler diets were formulated. Diet A served as control with 23, 22 and 20% CP, while in diets B, C and D, the CP was reduced to 22, 21 and 20%; 21, 20 and 19% and 19, 18 and 17% with 2925, 3075 and 3125 kcal/kg energy for starter, grower and finisher periods, respectively. A total of 1760, one day-old Hubbard broiler chicks were randomly divided into 16 experimental units, 110 chicks in each unit and each diet was offered to four experimental units at random. Results suggested that N excretion decreased (P<0.05), while N retention and its excretion as percent of N intake were not different among treatments. Low CP diets significantly reduced (P<0.05) the moisture and N content of the litter; although ash content was not altered. The plasma uric acid concentration decreased (P<0.05) and triglycerides concentration increased significantly by lowering the dietary CP content. However plasma glucose, triiodothyronine and thyroxine concentrations were unaffected. In conclusion, lowering dietary CP resulted in reduced N excretion without any adverse effect on N retention, which was also expressed in the levels of plasma metabolites and hormones. © 2010 Friends Science Publishers
Object Classifications by Image Super-Resolution Preprocessing for Convolutional Neural Networks

A R T I C L E I N F O A B S T R A C T Article history: Received: 25 January, 2020 Accepted: 12 March, 2020 Online: 04 April, 2020 Blurred small objects produced by cropping, warping, or intrinsically so, are challenging to detect and classify. Therefore, much recent research is focused on feature extraction built on Faster R-CNN and follow-up systems. In particular, RPN, SPP, FPN, SSD, and DSSD are the layered feature extraction methods for multiple object detections and small objects. However, super-resolution methods, as explored here, can improve these image analyses working on before or after convolutional neural networks. Our methods are focused on building better image qualities into the original image components so that these feature extraction methods become more effective when applied later. Our super-resolution preprocessing resulted in better deep learning in the number of classified objects, especially for small objects when tested on the VOC2007, MSO, and COCO2017 datasets.
An unusual new genus of istiodactylid pterosaur from China based on a near complete specimen

A new genus and species of istiodactylid pterosaur, Luchibang xingzhe gen. et sp. nov., from the Lower Cretaceous Yixian Formation of China is erected based on a near complete specimen lacking only the posterior of the skull and the tail. The holotype individual is skeletally immature and already bigger than most istiodactylids suggesting a large wingspan at adult. This is the most complete istiodactylid specimen known to date and provides new information on the anatomy of this group and their putative ecology. The animal is unusual in having postcranial robust and elongate hindlimbs, which marks it as different to other istiodactylids and other pteranodontoids. David W. E. Hone. Queen Mary University of London, Mile End Road, London, E1 4NS, UK. d.hone@qmul.ac. uk Adam J. Fitch. Department of Geosciences, University of Wisconsin-Madison, 1215 West Dayton St, Madison, Wisconsin 53105, USA. afitch2@wisc.edu Feimin Ma. School of the Earth Sciences and Resources, China University of Geosciences, 28 Xueyuan Road, Haidian District, 100083 Beijing, China. Ma@sesr.sn Xing Xu. Institute of Vertebrate Palaeontology and Palaeoanthropology, Xizhimenwai Dajie 142, 100044 Beijing, China. xu.xing@ivpp.ac. cn and CAS Center of Excellence in Life and Paleoenvironment, Beijing, 100044, China.
Aerococcus, Abiotrophia, and Other Aerobic Catalase-Negative, Gram-Positive Cocci

The aerobic catalase-negative gram-positive cocci included in this chapter form a taxonomically diverse group of bacteria that are isolated infrequently as opportunistic agents of infection. The genera Abiotrophia and Granulicatella accommodate organisms previously known as nutritionally variant or satelliting streptococci. Most of the genera described here are catalase-negative facultative anaerobes, but Aerococcus viridans are classified as a microaerophile that grows poorly, if at all, under anaerobic conditions. Some strains of Aerococcus may exhibit weakly positive catalase reactions due to nonheme catalase activity. Commercially available identification kits or systems offering a more comprehensive array of phenotypic tests are improving in their ability to identify many of the organisms discussed in the chapter. Serologic response to the organisms described in this chapter has not been extensively investigated. Antimicrobial susceptibility studies on the organisms mentioned in the chapter have generally employed dilution testing methods. The lack of standardized methods and interpretive criteria and the relatively small collections of isolates for some of the genera discussed in the chapter make it difficult to accurately assess antimicrobial susceptibility patterns. Efforts to identify the gram-positive cocci included in the chapter should be made only when isolates are considered to be clinically significant, since the organisms may also appear in clinical cultures as contaminants or constituents of the normal microbiota. More extensive phenotypic testing using commercially available identification systems and molecular methods should be employed for definitive identification.
Effect of Forage Brassica on Subsequent Soil Water Content and Yield of Dual-Purpose Winter Wheat in Rainfed Region of Northwestern China

Part of the Plant Sciences Commons, and the Soil Science Commons This document is available at https://uknowledge.uky. edu/igc/22/2-10/9 The 22nd International Grassland Congress (Revitalising Grasslands to Sustain Our Communities) took place in Sydney, Australia from September 15 through September 19, 2013. Proceedings Editors: David L. Michalk, Geoffrey D. Millar, Warwick B. Badgery, and Kim M. Broadfoot Publisher: New South Wales Department of Primary Industry, Kite St., Orange New South Wales, Australia
Effect of Drip and Mulch on Growth, Yield and WUE of Tomato under Low Cost Polyhouse in Sikkim Condition

Tomato is considered as one of the most productive as well as protective food because of its nutritive value, widespread production and consumed vegetables worldwide. In India, it is grown in the wide range of climatic condition and ranks 2 nd largest nation in production worldwide (Reddy M. S. et al., 2018). It is consumed in many ways and most common ingredient in dishes, sauces, salads and drinks. It is a warm season crop, temperature below 10 ̊C and above 38 ̊C may adversely affect the plant tissues thereby effecting the plants physiological activities.