Miracle-dz/UniFAR-FTUs huggingface dataset:
Emotional Availability and Play in Mother–Child Dyads with ASD: Changes during a Parental Based Intervention

(1) Background: Parental involvement during intervention with children with Autism Spectrum Disorder (ASD) has been demonstrated to be fundamental for children’s developmental outcomes. However, most research focused on child gains especially considering cognitive functioning and symptoms severity, whereas parental and dyadic changes during intervention need further investigation. (2) Methods: 29 mothers in interaction with their preschool children with ASD were analyzed through two standardized behavioral and observational measures to evaluate the dyadic Emotional Availability (EA) and play skills before (T1) and after (T2) a parental-based intervention. (3) Results: Results revealed mothers increased affective quality and major awareness in understanding the signals produced by the child, that in turn was more responsive, involving also using more complex play strategies. Interestingly, the role of specific factors able to predict parental characteristics was investigated, pointing out the important contribution of mothers’ perceptions of having a difficult child and child language communicative abilities. (4) Conclusions: the study enhances knowledge about child and caregiver variables that impact on dyadic outcomes, identifying important target areas to be addressed during intervention. Further, our results suggest that a parental-based intervention supports and facilitates improvements in both children’s and caregivers’ affective quality and cognitive abilities.
Healers Need Healing Too: Results from the Good Road of Life Training.

Mental health professionals that work with American Indian and Alaska Native (AI/AN) populations are often viewed as ineffective because their professional training is based on a Western model of service delivery that is an extension of Western colonization. Research on effective training approaches for AI/AN mental health providers or mental health professionals that work with AI/AN populations is limited. The purpose of this study is to document the experiences and impact of the Good Road of Life (GRL) training on mental health professionals that work with AI/AN populations. A cross sectional mixed-methods design was used to answer the primary research question, "What is the impact of GRL training on mental health professionals who work in American Indian communities? " We used GRL ratings, self-reported impacts, knowledge gains, and pre-post Sources of Strength scores. Self-reported mean knowledge scores increased for all topics. Daily evaluations indicate that most participants felt more positive, knew more about the impacts of sobriety, and knew how to help a suicidal person. Sources of Strength mean scores increased in the following areas: confidence, belonging, historical trauma, using strengths to overcome difficulties, spiritual practices, resolved unhealthy relationships, and use of cultural resilience. Results indicate that GRL is an effective short-term training for professionals working in the mental health field throughout Indian Country.
Combining High-Pressure Perturbation with NMR Spectroscopy for a Structural and Dynamical Characterization of Protein Folding Pathways

High-hydrostatic pressure is an alternative perturbation method that can be used to destabilize globular proteins. Generally perfectly reversible, pressure exerts local effects on regions or domains of a protein containing internal voids, contrary to heat or chemical denaturant that destabilize protein structures uniformly. When combined with NMR spectroscopy, high pressure (HP) allows one to monitor at a residue-level resolution the structural transitions occurring upon unfolding and to determine the kinetic properties of the process. The use of HP-NMR has long been hampered by technical difficulties. Owing to the recent development of commercially available high-pressure sample cells, HP-NMR experiments can now be routinely performed. This review summarizes recent advances of HP-NMR techniques for the characterization at a quasi-atomic resolution of the protein folding energy landscape.
Typhoid and Paratyphoid Cost of Illness in Bangladesh: Patient and Health Facility Costs From the Surveillance for Enteric Fever in Asia Project II

Abstract Background We conducted a cost of illness study to assess the economic burden of pediatric enteric fever (typhoid and paratyphoid) in Bangladesh. Results can inform public health policies to prevent enteric fever. Methods The study was conducted at 2 pediatric health facilities in Dhaka. For the patient and caregiver’s perspective, we administered questionnaires on costs incurred from illness onset until the survey dates to caregivers of patients with blood culture positive cases at enrollment and 6 weeks later to estimate the direct medical, direct nonmedical, and indirect costs. From the perspective of the health care provider, we collected data on quantities and prices of resources used by the 2 hospitals to estimate the direct medical economic costs to treat a case of enteric fever. We collected costs in Bangladeshi takas and converted them into 2018 US dollars. We multiplied the unit cost per procedure by the frequency of procedures in the surveillance case cohort to calculate the average cost per case. Results Among the 1772 patients from whom we collected information, the median cost of illness per case of enteric fever from the patient and caregiver perspective was US $64.03 (IQR: US $33.90 –$173.48). Median direct medical and nonmedical costs per case were 3% of annual labor income across the sample. From the perspective of the healthcare provider, the average direct medical cost per case was US $58.64 (range: US $37.25 at Hospital B, US $73.27 at Hospital A). Conclusions Our results show substantial economic burden of enteric fever in Bangladesh, with higher costs for patients receiving inpatient care. As antimicrobial resistance increases globally, the cost of illness could increase, due to more expensive and potent drugs required for treatment.
Best Procedure for the Management of Common Bile Duct Stones via the Papilla: Literature Review and Analysis of Procedural Efficacy and Safety

Background: Endoscopic management of common bile duct stones (CBDS) is standard; however, various techniques are performed via the papilla, and the best procedure in terms of both efficacy and safety has not been determined. Methods: Endoscopic procedures were classified into five categories according to endoscopic sphincterotomy (EST) and balloon dilation (BD): (1) EST, (2) endoscopic papillary BD (≤10 mm) (EPBD), (3) EST followed by BD (≤10 mm) (ESBD), (4) endoscopic papillary large BD (≥12 mm) (EPLBD), and (5) EST followed by large BD (≥12 mm) (ESLBD). We performed a literature review of prospective and retrospective studies to compare efficacy and adverse events (AEs). Each procedure was associated with different efficacy and AE profiles. Results: In total, 19 prospective and seven retrospective studies with a total of 3930 patients were included in this study. For EST, the complete stone removal rate at the first session, rate of mechanical lithotripsy (ML), and rate of overall AEs in EST were superior to EPBD, but a higher rate of bleeding was found for EST. Based on one retrospective study, complete stone removal rate at the first session, rate of ML, and rate of overall AEs were superior for ESBD vs. EST, and the rate of bleeding for the former was also lower. Complete stone removal rate at the first session and rate of ML for ESLBD were superior to those for EST, with no significant difference in rate of AEs. For EST vs. EPLBD, complete stone removal rate at the first session and rate of ML were superior for the latter. For EPLBD vs. ESLBD, the efficacy and safety were similar. Conclusions: ESBD is considered the best procedure for the management of small CBDS, but strong evidence is lacking. For large CBDS, both ESLBD and EPLBD are similar.
The Protective Effects of the Autophagic and Lysosomal Machinery in Vascular and Valvular Calcification: A Systematic Review

Background: Autophagy is a highly conserved catabolic homeostatic process, crucial for cell survival. It has been shown that autophagy can modulate different cardiovascular pathologies, including vascular calcification (VCN). Objective: To assess how modulation of autophagy, either through induction or inhibition, affects vascular and valvular calcification and to determine the therapeutic applicability of inducing autophagy. Data sources: A systematic review of English language articles using MEDLINE/PubMed, Web of Science (WoS) and the Cochrane library. The search terms included autophagy, autolysosome, mitophagy, endoplasmic reticulum (ER)-phagy, lysosomal, calcification and calcinosis. Study characteristics: Thirty-seven articles were selected based on pre-defined eligibility criteria. Thirty-three studies (89%) studied vascular smooth muscle cell (VSMC) calcification of which 27 (82%) studies investigated autophagy and six (18%) studies lysosomal function in VCN. Four studies (11%) studied aortic valve calcification (AVCN). Thirty-four studies were published in the time period 2015–2020 (92%). Conclusion: There is compelling evidence that both autophagy and lysosomal function are critical regulators of VCN, which opens new perspectives for treatment strategies. However, there are still challenges to overcome, such as the development of more selective pharmacological agents and standardization of methods to measure autophagic flux.
A review and reappraisal of the specific gravities of present and past multicellular organisms, with an emphasis on tetrapods

The density, or specific gravity (SG), of organisms has numerous important implications for their form, function, ecology, and other facets of beings living and dead, and it is especially necessary to apply SG values that are as accurate as practical when estimating their masses which is itself a critical aspect of living things. Yet a comprehensive review and analysis of this notable subject of anatomy has never been conducted and published. This is such an effort, being as extensive as possible with the data on hand, bolstered by some additional observations, and new work focusing on extinct animals who densities are least unknown: pterosaurs and dinosaurs with extensive pneumatic complexes, including the most sophisticated effort to date for a sauropod. Often difficult to determine even via direct observation, techniques for obtaining the best possible SG data are explained and utilized, including observations of floating animals. Neutral specific gravity (NSG) is proposed as the most important value for tetrapods with respiratory tracts of fluctuating volume. SGs of organisms range from 0.08 to 2.6, plant tissues from 0.08 to 1.39, and vertebrates from about 0.75 (some giant pterosaurs) to 1.2 (those with heavy armor and/or skeletons). Tetrapod NSGs tend to be somewhat higher than widely thought, especially those theropod and sauropod dinosaurs and pterosaurs with air‐sacs because respiratory system volume is usually measured at maximum inhalation in birds. Also discussed is evidence that the ratio of the mass of skeletons relative to total body mass has not been properly assayed in the past.
Lung transplantation for patients with severe COVID-19

Some patients with severe COVID-19 develop end-stage pulmonary fibrosis for which lung transplantation may be the only treatment. A surgical solution for severe COVID-19 Despite optimal medical therapy, some patients with severe COVID-19 develop irreversible lung injury. In these patients who cannot be weaned from mechanical ventilation or extracorporeal life support, lung transplantation may be the only life-saving option. Bharat et al. now report the results of lung transplantation in three patients who had COVID-19–associated respiratory failure. SARS-CoV-2 RNA could not be detected in the explanted lungs of these patients, but fibrotic pathology and transcriptional changes resembling those of lungs from patients with pulmonary fibrosis were observed. Lung transplantation can potentially be a life-saving treatment for patients with nonresolving COVID-19–associated respiratory failure. Concerns limiting lung transplantation include recurrence of SARS-CoV-2 infection in the allograft, technical challenges imposed by viral-mediated injury to the native lung, and the potential risk for allograft infection by pathogens causing ventilator-associated pneumonia in the native lung. Additionally, the native lung might recover, resulting in long-term outcomes preferable to those of transplant. Here, we report the results of lung transplantation in three patients with nonresolving COVID-19–associated respiratory failure. We performed single-molecule fluorescence in situ hybridization (smFISH) to detect both positive and negative strands of SARS-CoV-2 RNA in explanted lung tissue from the three patients and in additional control lung tissue samples. We conducted extracellular matrix imaging and single-cell RNA sequencing on explanted lung tissue from the three patients who underwent transplantation and on warm postmortem lung biopsies from two patients who had died from COVID-19–associated pneumonia. Lungs from these five patients with prolonged COVID-19 disease were free of SARS-CoV-2 as detected by smFISH, but pathology showed extensive evidence of injury and fibrosis that resembled end-stage pulmonary fibrosis. Using machine learning, we compared single-cell RNA sequencing data from the lungs of patients with late-stage COVID-19 to that from the lungs of patients with pulmonary fibrosis and identified similarities in gene expression across cell lineages. Our findings suggest that some patients with severe COVID-19 develop fibrotic lung disease for which lung transplantation is their only option for survival.
Re-injury worry, confidence and attention as predictors of a sport re-injury during a competitive season

ABSTRACT Rehabilitation from sport injury involves not only physical, but also psychological considerations. Re-injury worry, confidence and attention are characteristics that may affect athletes’ returning to competition after a sport injury. The study aimed to describe the predictive ability of the re-injury worry, confidence, and attention to the total of re-injuries at the new competitive season. The sample was 80 male athletes with at least three years of competitive experience who had a sport musculoskeletal injury in the last 8 months and had followed properly their physiotherapy program. They were ready for re-entry into competitive sport. They completed on returning to competition: (a) the Causes of the Re-Injury Worry Questionnaire, (b) the Sport Confidence Questionnaire of Rehabilitated Athletes Returning to Competition and (c) the Attention Questionnaire of Rehabilitated Athletes Returning to Competition. The three valid and reliable instruments predict the total of re-injuries at the beginning and at the middle of the competitive season. Future research should be conducted to examine the relationship between the physical and psychological characteristics in re-injury’s prediction from different contact sports.
Chronic Ethanol Exposure during Adolescence Increases Voluntary Ethanol Consumption in Adulthood in Female Sprague Dawley Rats

Early alcohol use is a major concern due to the dramatic rise in alcohol use during adolescence. In humans, adolescent males and females consume alcohol at equivalent rates; however, in adulthood males are more likely to consume harmful levels of alcohol. In animal models, the long-term dose-dependent and sex-dependent effects of alcohol exposure during adolescence have not been readily assessed relative to exposure that is initiated in adulthood. The purpose of the present set of experiments was to determine if adolescent exposure to chronic ethanol would predispose male and female rats to greater ethanol intake in adulthood when compared to animals that were not exposed to chronic ethanol exposure until early adulthood. Male and female rats were chronically administered 0.75 g/kg or 1.5 g/kg ethanol or saline for 21 days during adolescence (postnatal day (PND) 30–50) or adulthood (PND 60–80). All rats subsequently underwent 14-days of abstinence (PND 51–64 or PND 81–94, respectively). Finally, all rats were given 30-min daily access to saccharin-sweetened ethanol or saccharin alone from PND 65–80 for adolescent-exposed rats and PND 95–110 for adult-exposed rats. Exposure to 0.75 g/kg ethanol did not alter ethanol or saccharin intake in adolescent-exposed or adult-exposed rats, regardless of sex. In contrast, chronic exposure to the higher 1.5 g/kg dose during adolescence increased ethanol intake in adulthood in female rats. However, there was no change in saccharin intake in animals exposed to 1.5 g/kg ethanol during adolescence or adulthood, regardless of sex. Additionally, there were no clear age- and ethanol-dependent changes in duration of loss of righting reflex and blood ethanol concentrations to a challenge administration of a higher dose of ethanol. The results of the present set of experiments indicate chronic exposure to a high dose of ethanol during adolescence in female rats did indeed predispose rats to consume more ethanol in adulthood. Given that these effects were only observed in adolescent-exposed female rats, these results support a unique vulnerability to the long-term consequences of adolescent ethanol exposure in female rats, an effect that is not merely mediated by the sweetener used in the ethanol solution.
The Efficacy of School-Based Interventions in Preventing Adolescent Obesity in Australia

Current trends suggest that adolescent obesity is an on-going and recurrent decimal that is still on the rise in Australia and the social burden associated with it can significantly cause low self-esteem and lack of confidence in personal body image in adulthood. Nonetheless, evidence-based prevention programs are not widely implemented in schools, even though they are commonplace for easy access to adolescents. The primary objective of this systematic review was to assess the scope and efficacy of adolescent obesity intervention strategies in Australian schools, to guide future research. Seven electronic databases were searched for peer-reviewed school-based intervention articles written in the English language and targeting 12–18-year-old adolescents. Intervention characteristics were extracted, and quality, efficacy and outcome measures were assessed utilizing thirteen studies that met the inclusion criteria for this review. Most of the Australian adolescent obesity research emanated from the State of New South Wales and none were nationwide. Five studies successfully met all the requirements in all measured outcomes, four met at least one measured outcome and the remaining four were unsuccessful. Despite the weak evidence of intervention efficacy for most of the reviewed studies, school-based interventions with multi-component combinations of physical activity, nutrition and alignment to a theory yielded promising results. Our findings point to the need for future research to assess the perceptions of school stakeholders in relation to the barriers and enablers to establishing school-based prevention and intervention programs for adolescents.
The authors reply.

Critical Care Medicine www.ccmjournal. org e1369 of the study may not be applicable to older children where the most common cause of ARF is pneumonia or acute respiratory distress syndrome. The reliability of PedsQL is also debatable as this score is generally validated for children greater than 2 years (2). The positive respiratory bacterial culture was noted in 70% children (n = 140), whereas majority of children (74%) had acute bronchiolitis. The significance of positive bacterial culture in children with acute bronchiolitis is not known. Also, there is lack of information on the timing of sampling, type of sample, other markers of infection, and whether it is community acquired or ventilator-associated pneumonia. In absence of these answers, the significance of a positive respiratory bacterial culture and its prediction of PRM at 6 and 12 months of follow-up are doubtful. The clinical diagnosis of acute bronchiolitis was noted in 74% cases (n = 189), and 75% (144/193) had positivity for respiratory syncytial virus (RSV). As we know that the RSV bronchiolitis is associated with increased risk of allergic sensitization, recurrent wheezing, and reactive airway disease (3, 4), it is likely that most of the outcome measures of PRM were actually related to reactive airway disease linked to RSV infection and may not be specifically related to invasive mechanical ventilation. As highlighted by authors, the higher proportion of parents of children with PRM could have responded to telephone calls giving a higher rate of PRM of 42–44%. A study from authors unit enrolled 29 children (5–12 yr) with acute hypoxemic respiratory failure who underwent invasive mechanical ventilation and survived. These were followedup at 3 and 9–12 months after discharge for clinical signs and symptoms of respiratory involvement, pulse oximetry, chest radiograph, 6-minute walk test, peak expiratory flow rate, and spirometry (5). During first follow-up, 37.9% (11/29) had recurrent respiratory symptoms, but none had during second follow-up. None of the children had limitation of physical activity or need of supplemental oxygen. Fifty five percent of children returned to schooling in the first follow-up and 85.2% in second follow-up. The 6-minute walk test was successfully performed by 96.5% and 100% children during first and second follow-up, respectively, with significant increase in distance walked from first to second follow-up. The peak expiratory flow rate less than 80% of predicted was noted in 20.7% and 14.8% children, and abnormal spirometry in 82.7% and 18.5% during first and second follow-ups, respectively. There was no correlation between pulmonary functions and lung injury scores, oxygenation indices (Pao 2 /Fio 2 ratio and oxygenation index), and mechanical ventilation variables. Large well-designed multicentric studies are needed to define long-term pulmonary morbidity among children undergoing invasive mechanical ventilation for ARF including broad range of age groups and common pulmonary diseases causing ARF using objective variables. The authors have disclosed that they do not have any potential conflicts of interest.