Miracle-dz/UniFAR-FTUs huggingface dataset:
V1309 Sco—UNDERSTANDING A MERGER

One of the two outcomes of a common envelope (CE) event is a merger of the two stars. To date, the best known case of a binary merger is the V1309 Sco outburst, where the orbital period was known and observed to decay up to the outburst. Using the hydrodynamical code StarSmasher, we study in detail which characteristics of the progenitor binary affect the outburst and produce the best match with observations. We have developed a set of tools in order to quantify any CE event. We consider binaries consisting of a 1.52 M☉ giant and a 0.16 M☉ companion with Porb ∼ 1.4 days, varying the nature of the companion and its synchronization. We show that all considered progenitor binaries evolve toward the merger primarily because of Darwin instability. The merger is accompanied by mass ejection that proceeds in several separate mass outbursts and takes away a few percent of the donor mass. This very small mass, nonetheless, is important as it is not only sufficient to explain the observed light curve, but it also carries away up to one-third of both the initial total angular momentum and initial orbital energy. We find that all synchronized systems experience L2 mass loss that operates during just a few days prior to the merger and produces ring-shaped ejecta. The formed star is always a strongly heated radiative star that differentially rotates. We conclude that the case of a synchronized binary with a main-sequence companion gives the best match with observations of V1309 Sco.
MULTIPLE INVERSE COMPTON SCATTERINGS AND THE BLAZAR SEQUENCE

The high frequency component in blazars is thought to be due to inverse Compton scattered radiation. Recent observations by Fermi-LAT are used to evaluate the details of the scattering process. A comparison is made between the usually assumed single scattering scenario and one in which multiple scatterings are energetically important. In the latter case, most of the radiation is emitted in the Klein-Nishina limit. It is argued that several of the observed correlations defining the blazar sequence are most easily understood in a multiple scattering scenario. Observations indicate also that, in such a scenario, the blazar sequence is primarily governed by the energy density of relativistic electrons rather than that of the seed photons. The pronounced X-ray minimum in the spectral energy distribution often observed in the most luminous blazars is discussed. It is shown how this feature can be accounted for in a multiple scattering scenario by an extension of standard one-zone models.
Social investigation and social novelty in zebrafish: Roles of salience and novelty

Social preference tests can be used to analyze variables that influence and modify social behaviors, and to investigate effects of substances such as medications, drugs, and hormones. They may become important tools for finding a valid model to study neuropsychiatric changes and to study human neurodevelopmental processes that have been impaired by social events. While a preference for conspecifics has been shown for different species, social novelty has been used as a model for anxiety-like behavior in rodents. The goal of this research was to understand the roles of stimulus salience (numerousness) and novelty in social investigation and social novelty tests in zebrafish (Danio rerio Hamilton 1822). We used a sequential design, in which animals are exposed first to a social investigation test (with dichotomous presentation of novel conspecifics vs. empty tank) and then to a social novelty test (with dichotomous presentation of the already known conspecific and a novel conspecific). In experiment 1, animals were presented to either 1 or 3 (vs. an empty tank) conspecifics as stimuli. In experiment 2, animals were presented to 1 vs. 3 conspecifics as stimuli. In experiment 3, animals were observed in the social investigation and social novelty tests for 3 consecutive days. The results showed equivalence between 1 or 3 conspecifics in the social investigation and social novelty tests, although animals were able to discriminate between different shoal sizes. These preferences do not change with repeated test exposure, suggesting novelty to be a minor contributor to social investigation and social novelty in zebrafish.
Activin A promotes bone fracture repair and acts through a novel myofibroblastic cell population in callus

Insufficient bone fracture repair represents a significant clinical burden, and identification of novel therapeutics enhancing repair would have substantial clinical and societal impact. Activin A is a TGF-β protein superfamily member known to stimulate ectopic bone formation, but its roles in fracture repair and its therapeutic potentials remain unclear. Using two mouse tibia fracture repair models, here we mapped activin A expression at the tissue and single cell levels, tested its requirement for normal repair and evaluated its ability to enhance repair when provided exogenously. Activin A was minimally expressed in periosteum of intact bones but was markedly upregulated in developing callus soon after fracture. Single cell RNA-sequencing revealed that the activin A-encoding gene Inhba marked a unique, highly proliferative progenitor cell (PPC) population with a myofibroblast character which emerged over repair time and lay at the center of a developmental trajectory bifurcation producing cartilage and bone cells within callus. Systemic administration of a neutralizing activin A antibody impaired fracture repair and its endochondral and intramembranous phases, whereas local delivery of recombinant activin A enhanced repair. Activin A delivery also induced SMAD2 phosphorylation in vivo and increased the fraction of αSMA+ myofibroblasts within fracture callus. Gain- and loss-of-function experiments in vitro showed that activin A directly stimulated myofibroblast differentiation, chondrogenesis and osteogenesis in periosteal progenitor cells. Together, these data identify a unique population of Inhba-expressing proliferative progenitor cells that give rise to chondrocytes and osteoblasts during fracture healing and establish activin A as a potential new therapeutic tool to enhance it. One Sentence Summary Deficits in bone fracture repair remain a clinical challenge and the present study provides evidence for the therapeutic potentials of activin A
A geometric approach to quantifying the neuromodulatory effects of persistent inward currents on single motor unit discharge patterns

Objective All motor commands flow through motoneurons, which entrain control of their innervated muscle fibers, forming a motor unit (MU). Owing to the high fidelity of action potentials within MUs, their discharge profiles detail the organization of ionotropic excitatory/inhibitory as well as metabotropic neuromodulatory commands to motoneurons. Neuromodulatory inputs (e.g., norepinephrine, serotonin) enhance motoneuron excitability and facilitate persistent inward currents (PICs). PICs introduce quantifiable properties in MU discharge profiles by augmenting depolarizing currents upon activation (i.e., PIC amplification) and facilitating discharge at lower levels of excitatory input than required for recruitment (i.e., PIC prolongation). Approach: Here, we introduce a novel geometric approach to estimate neuromodulatory and inhibitory contributions to MU discharge through exploiting discharge non-linearities introduced by PIC amplification during time-varying linear tasks. In specific, we quantify the deviation from linear discharge (“brace height”) and the rate of change in discharge (i.e., acceleration slope, attenuation slope, angle). We further characterize these metrics on a simulated motoneuron pool with known excitatory, inhibitory, and neuromodulatory inputs and on human MUs (Tibialis Anterior: 1448, Medial Gastrocnemius: 2100, Soleus: 1062, First Dorsal Interosseus: 2296). Main Result In the simulated motor pool, we found brace height and attenuation slope to consistently indicate changes in neuromodulation and the pattern of inhibition (excitation-inhibition coupling), respectively, whereas the paired MU analysis (ΔF) was dependent on both neuromodulation and inhibition pattern. Furthermore, we provide estimates of these metrics in human MUs and show comparable variability in ΔF and brace height measures in MUs matched across multiple trials. Significance Spanning both datasets, we found brace height quantification to provide an intuitive method for achieving graded estimates of neuromodulatory and inhibitory drive to MUs on a single unit level. This complements common techniques and provides an avenue for decoupling changes in the level of neuromodulatory and pattern of inhibitory motor commands.
Dissecting the cellular basis of age-related cognitive dysfunction: Chronic chemogenetic inhibition of somatostatin-positive interneurons in the dentate gyrus hilus induces memory impairments

The cellular basis of age-related impairments of hippocampal function is not fully understood. In order to evaluate the role of somatostatin-positive (Sst + ) interneurons in the dentate gyrus hilus in this process, we chemogenetically inhibited Sst + interneurons in the dentate gyrus (DG) hilus. Chronic chemogenetic inhibition (CCI) of these neurons resulted in increased c-Fos staining in the DG hilus, a decrease in the percentage of Gad67- and of Sst-expressing neurons in the DG, and increased microglial activation in DG, CA3, and CA1. Total dendritic length and spine density were reduced in DG and CA1, suggesting reduced dendritic complexity. Behaviorally, the recognition index in an object recognition task and the percentage of spontaneous alternations in the Y maze were decreased, while in both initial and reversal learning in the Morris water maze the latencies to find the hidden platform were increased, suggesting cognitive dysfunction. Our findings establish a causal role for a reduced function of Sst + interneurons in the DG hilus for cognitive decline and suggest that this reduced function may contribute to age-related impairments of learning and memory. Our CCI mice may represent a cellularly defined model of hippocampal aging.
Inhibition of the SARS-CoV-2 helicase at single-nucleotide resolution

The genome of SARS-CoV-2 encodes for a helicase called nsp13 that is essential for viral replication and highly conserved across related viruses, making it an attractive antiviral target. Here we use nanopore tweezers, a high-resolution single-molecule technique, to gain detailed insight into how nsp13 turns ATP-hydrolysis into directed motion along nucleic acid strands. We measured nsp13 both as it translocates along single-stranded DNA or unwinds short DNA duplexes. Our data confirm that nsp13 uses the inchworm mechanism to move along the DNA in single-nucleotide steps, translocating at ~1000 nt/s or unwinding at ~100 bp/s. Nanopore tweezers’ high spatio-temporal resolution enables observation of the fundamental physical steps taken by nsp13 even as it translocates at speeds in excess of 1000 nucleotides per second enabling detailed kinetic analysis of nsp13 motion. As a proof-of-principle for inhibition studies, we observed nsp13’s motion in the presence of the ATPase inhibitor ATPγS. Our data reveals that ATPγS interferes with nsp13’s action by affecting several different kinetic processes. The dominant mechanism of inhibition differs depending on the application of assisting force. These advances demonstrate that nanopore tweezers are a powerful method for studying viral helicase mechanism and inhibition.
MAST: Phylogenetic Inference with Mixtures Across Sites and Trees

Hundreds or thousands of loci are now routinely used in modern phylogenomic studies. Concatenation approaches to tree inference assume that there is a single topology for the entire dataset, but different loci may have different evolutionary histories due to incomplete lineage sorting, introgression, and/or horizontal gene transfer; even single loci may not be treelike due to recombination. To overcome this shortcoming, we introduce the mixture across sites and trees (MAST) model, which uses a mixture of bifurcating trees to represent multiple histories in a single concatenated alignment. The MAST model allows each tree to have its own topology, branch lengths, substitution model, nucleotide or amino acid frequencies, and model of rate heterogeneity across sites. We implemented the MAST model in a maximum-likelihood framework in the popular phylogenetic software, IQ-TREE. Simulations show that we can accurately recover the true model parameters, including branch lengths and tree weights (i.e. frequencies) for a given set of tree topologies. We also show that we can use standard statistical inference approaches to reject a single-tree model when data are simulated under multiple trees (and vice versa). We applied the MAST model to multiple primate datasets and found that it can recover the signal of incomplete lineage sorting in the Great Apes, as well as the asymmetry in minor trees caused by introgression among several macaque species. When applied to a dataset of four Platyrrhine species for which standard concatenated maximum likelihood and gene tree approaches disagree, we find that MAST gives the highest weight to the tree favored by gene tree approaches. These results suggest that the MAST model is able to analyse a concatenated alignment using maximum likelihood, while avoiding some of the biases that come with assuming there is only a single tree. The MAST model can therefore offer unique biological insights when applied to datasets with multiple evolutionary histories. We discuss how it can be extended in the future.
Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius

Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single particle cryo electron microscopy (cryoEM), cryo electron tomography (cryoET) and Alphafold2 predictions to generate an atomic model of the two-component S-layer of Sulfolobus acidocaldarius. The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesize that jackknife-like conformational changes, as well as pH-induced alterations in the surface charge of SlaA, play important roles in S-layer assembly.
Rap1 coordinates cell-cell adhesion and cytoskeletal reorganization to drive collective cell migration in vivo


Collective cell movements contribute to tissue development and repair, and spread metastatic disease. In epithelia, cohesive cell movements require reorganization of adherens junctions and the actomyosin cytoskeleton. However, the mechanisms that coordinate cell-cell adhesion and cytoskeletal remodelling during collective cell migration in vivo are unclear. We investigated the mechanisms of collective cell migration during wound healing in the Drosophila embryonic epidermis. Upon wounding, the cells adjacent to the wound internalize cell-cell adhesion molecules and polarize actin and the motor protein myosin II to form a supracellular cable around the wound that coordinates cell movements. The cable anchors at former tricellular junctions (TCJs) along the wound edge, and TCJs are reinforced during wound closure. We found that the small GTPase Rap1 was both necessary and sufficient for rapid wound repair. Rap1 promoted actomyosin polarization to the wound edge and E-cadherin accumulation at TCJs. Using embryos expressing a mutant form of the Rap1 effector Canoe/Afadin that cannot bind Rap1, we found that Rap1 signals through Canoe for adherens junction remodelling, but not for actomyosin cable assembly. Rap1 was necessary and sufficient for RhoA/Rho1 activation at the wound edge. Consistent with this, the RhoGEF Ephexin localized to the wound edge in a Rap1-dependent manner, and Ephexin was necessary for myosin polarization and rapid wound repair, but not for E-cadherin redistribution. Together, our data show that Rap1 coordinates the molecular rearrangements that drive embryonic wound healing and independently drives actomyosin cable assembly through Ephexin-Rho1, and E-cadherin redistribution through Canoe, thus enabling rapid collective cell migration in vivo.
Mitofusin-2 in nucleus accumbens D2-MSNs regulates social dominance and neuronal function

The nucleus accumbens (NAc) is a brain hub regulating motivated behaviors, including social competitiveness. Mitochondrial function in the NAc is critically implicated in the association between anxiety and social competitiveness, and the mitochondrial fusion protein mitofusin 2 (Mfn2) in NAc neurons has been shown to regulate anxiety-related behaviors. However, it remains unexplored whether accumbal Mfn2 levels also affect social behavior and whether Mfn2 actions in the emotional and social domain are driven by distinct cell types. Here, we found that subordinate-prone highly anxious rats show reduced accumbal Mfn2 levels and that Mfn2 overexpression promotes dominant behavior. In mice, selective Mfn2 downregulation in NAc dopamine D2 receptor-expressing medium spiny neurons (D2-MSNs) induced social subordination, accompanied by reduced mitochondrial function and decreased neuronal excitability. Instead, D1-MSN-targeted Mfn2 downregulation affected competitive ability only transiently mainly by increases in anxiety-like behaviors. Our results assign dissociable cell-type specific roles to Mfn2 in the NAc in modulating social dominance and anxiety.
UNBIASED MILLIMETER-WAVE LINE SURVEYS OF TW Hya AND V4046 Sgr: THE ENHANCED C2H AND CN ABUNDANCES OF EVOLVED PROTOPLANETARY DISKS

We have conducted the first comprehensive millimeter-wave molecular emission line surveys of the evolved circumstellar disks orbiting the nearby, roughly solar-mass, pre-main-sequence (T Tauri) stars, TW Hya (D = 54 pc) and V4046 Sgr AB (D = 73 pc). Both disks are known to retain significant residual gaseous components despite the advanced ages of their host stars (∼8 Myr and ∼21 Myr, respectively). Our unbiased broadband radio spectral surveys of the TW Hya and V4046 Sgr disks were performed with the Atacama Pathfinder Experiment 12 m telescope, and are intended to yield a complete census of the bright molecular emission lines in the range 275–357 GHz (1.1–0.85 mm). We find that lines of 12CO, 13CO, HCN, CN, and C2H, all of which lie in the higher frequency (>330 GHz) range, constitute the strongest molecular emission from both disks in the spectral region surveyed. The molecule C2H is detected here for the first time in both disks, as is CS in the TW Hya disk. The survey results also include the first measurements of the full suite of the hyperfine transitions of CN N = 3 → 2 and C2H N = 4 → 3 in both disks. Modeling of these CN and C2H hyperfine complexes in the spectrum of TW Hya indicates that the emission from both species is optically thick and may originate from very cold (≲10 K) disk regions. The latter result, if confirmed, would suggest the efficient production of CN and C2H in the outer disk and/or near the disk midplane. It furthermore appears that the fractional abundances of CN and C2H are significantly enhanced in these evolved protoplanetary disks, relative to the fractional abundances of the same molecules in the environments of deeply embedded protostars. These results, combined with previous determinations of the enhanced abundances of other species (such as HCO+) in T Tauri star disks, underscore the importance of properly accounting for high-energy (FUV and X-ray) radiation from the central T Tauri star when modeling protoplanetary disk gas chemistry and physical conditions.
Personality inﬂuences hyperbolic discounting

We gather survey evidence for the influence of the HEXACO personality traits on the phenomenon of hyperbolic discounting. We also consider the demographics of age, sex, income and education, and evaluate how these interact with personality and hyperbolic discounting. Due to a sampling tec hnique of “snowball , ” we assembled a sample of well-educated and relatively wealthy adults from both sexes. Most respondents escaped hyperbolic discounting, and for those affected there was no “magnitude effect. ” Those participants showing higher conscient iousness were less hyperbolic. Moreover, those more open to experience who were more extroverted at the same time were also less hyperbolic. We also detail how such personality traits influence hyperbolic discounting mediated by the demographics of age, sex, income and educational attainment. Thus, conscientiousness, openness to experience and extraversion are traits that contribute to rational decisions in intertemporal choice in our sample, in that participants with these personality traits are less hyperbolic.
Pollen viability and germination in Elaeis oleifera , Elaeis guineensis and their interspecific hybrid 1

and higher contents and carotenes Rivera Choo & Nesaretnam ABSTRACT The cultivated area for the hybrid in Colombia approximately ha, representing 6.4 the total area cultivated with palm in the country O G hybrids produce few male and have low what indicates certain Peduncular or cover characteristics affect Elaeis oleifera chromosomes are similar to those of E. guineensis , with close gene pools for the production of interspecific O x G hybrids. The pollen viability and germination of E. oleifera ‘Coarí’ and E. guineensis ‘La Mé’ were compared to their interspecific hybrid O x G (‘Coarí’ x ‘La Mé’). The pollen viability was determined by the acetocarmine staining method (0.5 %) and the pollen germination by in vitro incubation on agar-sucrose medium (1.2-11.0 g in 100 mL of distilled water). The pollen viability and germination of the ‘Coarí’ x ‘La Mé’ hybrid were significantly lower than those of their parents. The percentage of pollen viability by acetocarmine staining was higher than that of in vitro germination, indicating that not all pollen grains classified as viable germinated on the agar-sucrose medium. The pollen germination test is a more reliable indicator than the staining viability test, because the latter only reveals that the pollen contains the enzymes necessary to initiate germination, while the germination test determines the emission and development of the pollen tube.