Miracle-dz/UniFAR-FTUs huggingface dataset:
Using the Omaha System to Evaluate the Integration of Behavioral Health Services into Nurse-Led Primary Health Care

ABSTRACT Integrating behavioral health services into nurse-led primary care at one location ensures that individuals receive a comprehensive array of preventive and restorative services, based on their varying needs. A formative program evaluation of a federally funded behavioral health integration (BHI) project in a small nurse-led clinic used the Omaha System taxonomy to explore the changes in the documented practice of providers due to the BHI implementation. The evaluation provided evidence of the benefits of a collaborative care model to urban low-income, underserved, adults who were predominantly African American/Blacks.
Large Structural Shear Deformation and Failure Monitoring Using Bend Losses in Polymer Optical Fibre

Rapid identification of structural damage positions is essential to the post-disaster rehabilitation of structures and infrastructures. Large shear deformation, e.g., shear failure of bridge piers, shear-slip of slopes, and shear cracking of structural walls, is often the cause of structural instability. Distributed optical fibre sensing (DOFS) techniques have an advantage over point-based sensors in terms of spatial continuous structural condition monitoring. This paper presents the development of new measurement theory and algorithm to evaluate the structural shear deflection based on the large beam deflection and optical bend loss theories. The proposed technique adopted a photon-counting Optical Time Domain Reflectometer (ν-OTDR) with polymer optical fibres (POFs) which has a large deformation measurement range and high spatial resolution. In the experiment, shear deformation events can be successfully detected and evaluated from the proposed technique. When the normalised shear deformation is larger than 0.2, both the event locations and the magnitudes can be accurately determined. When normalised shear deformation is lesser than 0.2, the error in the magnitude evaluation increased, but the event location can be found with an absolute error <0.5 m. Multiple shear events can be treated as independent events when they are separated by more than 5 m. Various configurations of POFs attached to concrete beam specimens for rupture failure monitoring were also studied. The configuration that could maximise the POF curvature at the beam failure produced the largest ν-OTDR signals. In other configurations in which the POFs were only stretched at failure, the signals were less strong and were influenced by the POF-structure bonding strength.
Ergodic Capacity Analysis of Full Duplex Relaying in the Presence of Co-Channel Interference in V2V Communications

We analyze the ergodic capacity of a dual-hop full duplex amplify-and-forward (AF) vehicle-to-vehicle (V2V) cooperative relaying system over Nakagami-m fading channels. In this context, the impacts of self-interference (SI) at the relay and co-channel interference (CCI) at the destination are taken into account in this analysis. Precisely, based on the analysis of the moment generating function (MGF) of the signal-to-interference-plus-noise ratio (SINR), new exact and lower bound expressions for the ergodic capacity are derived. The ergodic capacity upper bound is also derived based on the asymptotic outage probability of the approximated SINR. Monte-Carlo simulation results are presented to corroborate the derived analytical results. Our results show the significant impact of the considered interferences on the system performance. It is shown that the ergodic capacity is degraded when the average SI at the relay and/or the average CCI at the destination is increased. This highlights the importance of taking these phenomena into account in the performance evaluation in order to assess the practical limit of full duplex relaying (FDR) cooperative wireless communications. Interestingly, it is also observed that FDR with SI and CCI still shows a higher ergodic capacity than the interference-free half duplex relaying, especially at medium to high signal-to-noise ratios (SNRs).
Polysaccharide-Based Formulations for Healing of Skin-Related Wound Infections: Lessons from Animal Models and Clinical Trials

Skin injuries constitute a gateway for pathogenic bacteria that can be either part of tissue microbiota or acquired from the environmental. These microorganisms (such as Acinetobacter baumannii, Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus) produce virulence factors that impair tissue integrity and sustain the inflammatory phase leading for establishment of chronic wounds. The high levels of antimicrobial resistance have limited the therapeutic arsenal for combatting skin infections. Thus, the treatment of non-healing chronic wounds is a huge challenge for health services worldwide, imposing great socio-economic damage to the affected individuals. This scenario has encouraged the use of natural polymers, such as polysaccharide, in order to develop new formulations (membranes, nanoparticles, hydrogels, scaffolds) to be applied in the treatment of skin infections. In this non-exhaustive review, we discuss the applications of polysaccharide-based formulations in the healing of infected wounds in animal models and clinical trials. The formulations discussed in this review were prepared using alginate, cellulose, chitosan, and hyaluronic acid. In addition to have healing actions per se, these polysaccharide formulations can act as transdermal drug delivery systems, controlling the release of active ingredients (such as antimicrobial and healing agents). The papers show that these polysaccharides-based formulations are efficient in controlling infection and improve the healing, even in chronic infected wounds. These data should positively impact the design of new dressings to treat skin infections.
Nuclear Magnetic Resonance of Single Stranded RNAs and DNAs of CAAU and UCAAUC as Benchmarks for Molecular Dynamics Simulations.

RNA and DNA are rapidly emerging as targets for therapeutics and as potential frameworks for nanotechnology. Accurate methods for predicting and designing structures and dynamics of nucleic acids would accelerate progress in these and other applications. Suitable approximations for modeling nucleic acids are being developed, but require validation against disparate experimental observations. Here, nuclear magnetic resonance spectra for RNA and DNA single strands, CAAU and UCAAUC, are used as benchmarks to test molecular dynamics simulations with AMBER force fields OL3 and ROC-RNA for RNA, and BSC1 for DNA. A detailed scheme for making comparisons is also presented. The results reflect recent progress in approximations and reveal remaining challenges.
Pediatric Pulmonology

Correspondence Ira Shah, Pediatric TB Clinic, Department of Pediatric Infectious Diseases, B J Wadia Hospital for Children, Mumbai 400012, India. Email: irashah@pediatriconcall.com Abstract Aim: To compare the pharmacokinetics of isoniazid (INH) at doses 5 and 10mg/kg/day. Methods: INH concentrations were estimated by high‐performance liquid chromatography in 24 Indian children aged 1 to 15 years on antituberculosis therapy. Blood samples were collected at 0, 2, 4, 6, 8 hours after administration of INH. Patients were randomly given INH at 5 or 10mg/kg/day and maximum concentrations (Cmax) and area under the curve (AUC(0‐8)) were determined in each group. The 2‐hour concentration of INH was used as Cmax for this study. Results: Mean (standard deviation) Cmax was reached in 2 hours and was 2.68 ± 1.19 μg/mL in 5mg/kg/day group and 8.86 ± 3.94 μg/mL in 10mg/kg/day group (P < .05). The normal therapeutic range at 2‐hour concentrations for INH in adults achieving good clinical response is between 3 and 5 μg/mL. Among 5mg/kg/day, only 4 (33%) patients had INH concentrations within the 2‐hour concentrations therapeutic range whereas in 10mg/kg/day group, 11 (91%) patients achieved Cmax higher than the 2‐hour concentrations therapeutic range and 1 (9%) patient had Cmax within the 2‐hour concentrations therapeutic range. The mean AUC(0‐8) in 5mg/kg/day group was 10.04 ± 6.12 and 35.93 ± 25.37 μg·h/mL in 10mg/kg/day group (P = .0001). Conclusion: Children on daily INH 10mg/kg/day have higher AUC and Cmax than the required therapeutic range whereas over 65% of children with daily 5mg/kg/day INH therapy failed to achieve the optimal therapeutic range.
Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17

An aerobic denitrification strain, Pseudomonas balearica RAD-17, was identified and showed efficient inorganic nitrogen removal ability. The average NO3−-N, NO2−-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was 6.22 mg/(L∙h), 6.30 mg/(L∙h), and 1.56 mg/(L∙h), respectively. Meanwhile, optimal incubate conditions were obtained through single factor experiments. For nitrogen removal pathways, the transcriptional results proved that respiratory nitrate reductases encoded by napA, which was primarily performed in aerobic denitrification and cell assimilation, were conducted by gluS and gluD genes for ammonium metabolism. In addition, adding the strain RAD-17 into actual wastewater showed obvious higher denitrification performance than in the no inoculum group (84.22% vs. 22.54%), and the maximum cell abundance achieved 28.5 ± 4.5% in a ratio of total cell numbers. Overall, the efficient nitrogen removal performance plus strong environmental fitness makes the strain RAD-17 a potential alternative for RAS (recirculating aquaculture system) effluent treatment.
JsrNet: A Joint Sampling–Reconstruction Framework for Distributed Compressive Video Sensing

Huge video data has posed great challenges on computing power and storage space, triggering the emergence of distributed compressive video sensing (DCVS). Hardware-friendly characteristics of this technique have consolidated its position as one of the most powerful architectures in source-limited scenarios, namely, wireless video sensor networks (WVSNs). Recently, deep convolutional neural networks (DCNNs) are successfully applied in DCVS because traditional optimization-based methods are computationally elaborate and hard to meet the requirements of real-time applications. In this paper, we propose a joint sampling–reconstruction framework for DCVS, named “JsrNet”. JsrNet utilizes the whole group of frames as the reference to reconstruct each frame, regardless of key frames and non-key frames, while the existing frameworks only utilize key frames as the reference to reconstruct non-key frames. Moreover, different from the existing frameworks which only focus on exploiting complementary information between frames in joint reconstruction, JsrNet also applies this conception in joint sampling by adopting learnable convolutions to sample multiple frames jointly and simultaneously in an encoder. JsrNet fully exploits spatial–temporal correlation in both sampling and reconstruction, and achieves a competitive performance in both the quality of reconstruction and computational complexity, making it a promising candidate in source-limited, real-time scenarios.
Mortality in Different Mountain Sports Activities Primarily Practiced in the Winter Season—A Narrative Review

Annually, millions of people engage in mountain sports activities all over the world. These activities are associated with health benefits, but concurrently with a risk for injury and death. Knowledge on death rates is considered important for the categorization of high-risk sports in literature and for the development of effective preventive measures. The death risk has been reported to vary across different mountain sports primarily practiced in the summer season. To complete the spectrum, the aim of the present review is to compare mortality rates across different mountain sports activities primarily practiced in winter. A comprehensive literature search was performed on the death risk (mortality) during such activities, i.e., alpine (downhill) skiing, snowboarding, cross-country skiing, ski touring, and sledging. With the exception of ski touring (4.4 deaths per 1 million exposure days), the mortality risk was low across different winter sports, with small activity-specific variation (0.3–0.8 deaths per 1 million exposure days). Traumatic (e.g., falls) and non-traumatic (e.g., cardiac death) incidents and avalanche burial in ski tourers were the predominant causes of death. Preventive measures include the improvement of sport-specific skills and fitness, the use of protective gear, well-targeted and intensive training programs concerning avalanche hazards, and sports-medical counseling for elderly and those with pre-existing diseases.
The Cell-Cycle Regulatory Protein p21CIP1/WAF1 Is Required for Cytolethal Distending Toxin (Cdt)-Induced Apoptosis

The Aggregatibacter actinomycetemcomitans cytolethal distending toxin (Cdt) induces lymphocytes to undergo cell-cycle arrest and apoptosis; toxicity is dependent upon the active Cdt subunit, CdtB. We now demonstrate that p21CIP1/WAF1 is critical to Cdt-induced apoptosis. Cdt induces increases in the levels of p21CIP1/WAF1 in lymphoid cell lines, Jurkat and MyLa, and in primary human lymphocytes. These increases were dependent upon CdtB’s ability to function as a phosphatidylinositol (PI) 3,4,5-triphosphate (PIP3) phosphatase. It is noteworthy that Cdt-induced increases in the levels of p21CIP1/WAF1 were accompanied by a significant decline in the levels of phosphorylated p21CIP1/WAF1. The significance of Cdt-induced p21CIP1/WAF1 increase was assessed by preventing these changes with a two-pronged approach; pre-incubation with the novel p21CIP1/WAF1 inhibitor, UC2288, and development of a p21CIP1/WAF1-deficient cell line (Jurkatp21−) using clustered regularly interspaced short palindromic repeats (CRISPR)/cas9 gene editing. UC2288 blocked toxin-induced increases in p21CIP1/WAF1, and JurkatWT cells treated with this inhibitor exhibited reduced susceptibility to Cdt-induced apoptosis. Likewise, Jurkatp21− cells failed to undergo toxin-induced apoptosis. The linkage between Cdt, p21CIP1/WAF1, and apoptosis was further established by demonstrating that Cdt-induced increases in levels of the pro-apoptotic proteins Bid, Bax, and Bak were dependent upon p21CIP1/WAF1 as these changes were not observed in Jurkatp21− cells. Finally, we determined that the p21CIP1/WAF1 increases were dependent upon toxin-induced increases in the level and activity of the chaperone heat shock protein (HSP) 90. We propose that p21CIP1/WAF1 plays a key pro-apoptotic role in mediating Cdt-induced toxicity.
Targeting Rho-associated coiled-coil forming protein kinase (ROCK) in cardiovascular fibrosis and stiffening

ABSTRACT Introduction: Pathological cardiac fibrosis, through excessive extracellular matrix protein deposition from fibroblasts and pro-fibrotic immune responses and vascular stiffening is associated with most forms of cardiovascular disease. Pathological cardiac fibrosis and stiffening can lead to heart failure and arrythmias and vascular stiffening may lead to hypertension. ROCK, a serine/threonine kinase downstream of the Rho-family of GTPases, may regulate many pro-fibrotic and pro-stiffening signaling pathways in numerous cell types. Areas covered: This article outlines the molecular mechanisms by which ROCK in fibroblasts, T helper cells, endothelial cells, vascular smooth muscle cells, and macrophages mediate fibrosis and stiffening. We speculate on how ROCK could be targeted to inhibit cardiovascular fibrosis and stiffening. Expert opinion: Critical gaps in knowledge must be addressed if ROCK inhibitors are to be used in the clinic. Numerous studies indicate that each ROCK isoform may play differential roles in regulating fibrosis and may have opposing roles in specific tissues. Future work needs to highlight the isoform- and tissue-specific contributions of ROCK in fibrosis, and how isoform-specific ROCK inhibitors in murine models and in clinical trials affect the pathophysiology of cardiac fibrosis and stiffening. This could progress knowledge regarding new treatments for heart failure, arrythmias and hypertension and the repair processes after myocardial infarction.
High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

As a promising biocatalyst, Yarrowia lipolytica lipase 2 (YlLip2) is limited in its industrial applications due to its low thermostability. In this study, a thermostable YlLip2 mutant was overexpressed in Pichia pastoris and its half-life time was over 30 min at 80 °C. To obtain a higher protein secretion level, the gene dosage of the mutated lip2 gene was optimized and the lipase activity was improved by about 89%. Then, the YlLip2 activity of the obtained strain further increased from 482 to 1465 U/mL via optimizing the shaking flask culture conditions. Subsequently, Hac1p and Vitreoscilla hemoglobin (VHb) were coexpressed with the YlLip2 mutant to reduce the endoplasmic reticulum stress and enhance the oxygen uptake efficiency in the recombinant strains, respectively. Furthermore, high-density fermentations were performed in a 3 L bioreactor and the production of the YlLip2 mutant reached 9080 U/mL. The results demonstrated that the expression level of the thermostable YlLip2 mutant was predominantly enhanced via the combination of these strategies in P. pastoris, which forms a consolidated basis for its large-scale production and future industrial applications.
Signaling Network of Forkhead Family of Transcription Factors (FOXO) in Dietary Restriction

Dietary restriction (DR), which is defined as a reduction of particular or total nutrient intake without causing malnutrition, has been proved to be a robust way to extend both lifespan and health-span in various species from yeast to mammal. However, the molecular mechanisms by which DR confers benefits on longevity were not yet fully elucidated. The forkhead box O transcription factors (FOXOs), identified as downstream regulators of the insulin/IGF-1 signaling pathway, control the expression of many genes regulating crucial biological processes such as metabolic homeostasis, redox balance, stress response and cell viability and proliferation. The activity of FOXOs is also mediated by AMP-activated protein kinase (AMPK), sirtuins and the mammalian target of rapamycin (mTOR). Therefore, the FOXO-related pathways form a complex network critical for coordinating a response to environmental fluctuations in order to maintain cellular homeostasis and to support physiological aging. In this review, we will focus on the role of FOXOs in different DR interventions. As different DR regimens or calorie (energy) restriction mimetics (CRMs) can elicit both distinct and overlapped DR-related signaling pathways, the benefits of DR may be maximized by combining diverse forms of interventions. In addition, a better understanding of the precise role of FOXOs in different mechanistic aspects of DR response would provide clear cellular and molecular insights on DR-induced increase of lifespan and health-span.
Longitudinal Substance Use and Biopsychosocial Outcomes Following Therapeutic Community Treatment for Substance Dependence

The Therapeutic Community (TC) model is considered an effective treatment for substance dependence, particularly for individuals with complex presentations. While a popular approach for this cohort across a number of countries, few studies have focussed on biopsychosocial and longer-term outcomes for this treatment modality. This study reports on substance use, dependence, and biopsychosocial outcomes up to 9 months post-exit from two TC sites. Methods: A longitudinal cohort study (n = 166) with two follow-up time points. Measures included substance use, dependence, subjective well-being, social functioning, and mental and physical health. Generalized Linear Models were employed to assess change over time. Results: At 9 months, 68% of participants reported complete 90-day drug abstinence. Alcohol frequency and quantity were reduced by over 50% at 9 months, with 32% of the sample recording 90-day abstinence at 9 months. Both alcohol and drug dependence scores were reduced by over 60%, and small to medium effect sizes were found for a range of psychosocial outcomes at 9 months follow-up, including a doubling of wellbeing scores, and a halving of psychiatric severity scores. Residents who remained in the TC for at least 9 months reported substantially better outcomes. Conclusions: With notably high study follow-up rates (over 90% at 9 months post-exit), these data demonstrate the value of the TC model in achieving substantial and sustained improvements in substance use and psychosocial outcomes for a cohort with severe substance dependence and complex presentations. Implications for optimal length of stay are discussed.
Comment on “Laboratory Measurement and Analysis of the Deteriorated Layer Permeability Coefficient of Soil–Cement Deteriorated in a Saline Environment”

The derivation of a formula to compute the permeability coefficient in the commented paper assumes that the mass flow is homogeneous in a homogeneous layer of a specimen. This assumption is not correct when there is also, at least, one heterogeneous layer. Moreover, a mathematical lapse was found on one equation, that would prevent the right computation of the permeability coefficient, even if the assumption was correct. Although this does not invalidate the major conclusions of the study and has not an outstanding effect on the presented results, for the sake of rigor and sound background for future studies in this field, corrections to the published formulas and model are proposed.
The Relationship Between Sensory Reactivity Differences and Anxiety Subtypes in Autistic Children

Autistic children are at greater risk of developing anxiety than their nonautistic peers. Sensory reactivity differences have been implicated as one of the risk factors. Specifically, sensory hyperreactivity has previously been linked to anxiety, including separation anxiety and specific phobia; however, minimal research has explored the influence of sensory hyporeactivity and seeking. Therefore, the present study examined the correlational relationship between sensory reactivity differences and anxiety subtypes in 41 autistic children aged between 3 and 14 years, using parent‐ and self‐reported measures. We found positive correlations between sensory hyperreactivity and total anxiety, separation anxiety and physical injury fears. However, when controlling for autism traits, we found sensory hyperreactivity to be related to physical injury fears and specific phobia, and sensory hyporeactivity to be related to lower total and social anxiety. We found no significant relationships between sensory seeking and anxiety. These results indicate that sensory hyperreactivity and hyporeactivity might be implicated in specific anxiety symptomology. Our results also indicate minimal agreement between parent‐ and self‐reported anxieties, which highlights the limitations of informant reports for anxiety and the pressing need for objective anxiety assessments for autistic children to be developed. Our findings have important implications for limiting the development of anxiety in autistic children and suggest that sensory reactivity differences should be considered when developing targeted interventions for certain anxiety disorders. Autism Res 2020, 13: 785–795. © 2019 The Authors. Autism Research published by International Society for Autism Research published by Wiley Periodicals, Inc.