Miracle-dz/UniFAR-FTUs huggingface dataset:
The Effects of Some Aditives on Production Performances in Laying Hens

Normal 0 21 false false false MicrosoftInternetExplorer4 The research was performed on 108 laying hens, Isa-Brown hybrid, divided in four groups, 27 heads/group. Prebiotic Actigen was supplemented in total mixed ratio (TMR) in group 1E using a proportion of 0.06%, prebiotic Bio-Mos in proportion of 0.1% (group 2E) and organic Se (Sel-Plex) in proportion of 0.04% (group 3E). The trial developed within 6 weeks, first laying stage (33 weeks - 39 weeks of age), respectively. The laying hens were individually weighted, in the beginning and in the end of the trial. During trial, the following issues were studied: evolution of body weight, laying intensity, average weight of eggs and forage consumption by egg. The use of prebiotics (Bio-Mos and Actigen) and organic selenium (Sel-Plex) in the mentioned doses determined an increase of laying intensity and average weight of eggs and reducing the feed conversion ratio for an egg, as compared to the control group (C). The results obtained confirm the favourable influence of prebiotics and organic selenium on the main production and consumption indices of laying hens in the first laying stage.
Serum Iron Level as a Potential Predictor of Coronavirus Disease 2019 Severity and Mortality: A Retrospective Study

Abstract Background Various types of pulmonary diseases are associated with iron deficiency. However, information on iron status in coronavirus disease 2019 (COVID-19) is scarce. Methods This study included 50 hospitalized patients with confirmed COVID-19. The role of serum iron in predicting severity and mortality of COVID-19 was evaluated. Results The most common symptoms of COVID-19 patients in this study were cough (82%), fever (64%), and chest distress (42%). Of the 50 patients, 45 (90%) patients had abnormally low serum iron levels (<7.8 μmol/L). The severity of COVID-19 was negatively correlated with serum iron levels before and after treatment and was positively correlated with C-reactive protein, serum amyloid A, D-dimer, lactate dehydrogenase, urea nitrogen, and myoglobin levels. Decreased serum iron level could predict the transition of COVID-19 from mild to severe and critical illness. Seven (53.8%) patients with a lower serum iron level after treatment in the critical group had died. There was a significant difference in posttreatment serum iron levels between COVID-19 survivors and nonsurvivors. Conclusions Serum iron deficiency was detected in the patients with COVID-19. The severity and mortality of the disease was closely correlated with serum iron levels. Low serum iron concentration was an independent risk factor for death in COVID-19 patients.
Return to Sport After Anterior Cruciate Ligament Injury: Panther Symposium ACL Injury Return to Sport Consensus Group

Background: A precise and consistent definition of return to sport (RTS) after anterior cruciate ligament (ACL) injury is lacking, and there is controversy surrounding the process of returning patients to sport and their previous activity level. Purpose: The aim of the Panther Symposium ACL Injury Return to Sport Consensus Group was to provide a clear definition of RTS after ACL injury and a description of the RTS continuum as well as provide clinical guidance on RTS testing and decision-making. Study Design: Consensus statement. Methods: An international, multidisciplinary group of ACL experts convened as part of a consensus meeting. Consensus statements were developed using a modified Delphi method. Literature review was performed to report the supporting evidence. Results: Key points include that RTS is characterized by achievement of the preinjury level of sport and involves a criteria-based progression from return to participation to RTS and, ultimately, return to performance. Purely time-based RTS decision-making should be abandoned. Progression occurs along an RTS continuum, with decision-making by a multidisciplinary group that incorporates objective physical examination data and validated and peer-reviewed RTS tests, which should involve functional assessment as well as psychological readiness. Consideration should be given to biological healing, contextual factors, and concomitant injuries. Conclusion: The resultant consensus statements and scientific rationale aim to inform the reader of the complex process of RTS after ACL injury that occurs along a dynamic continuum. Research is needed to determine the ideal RTS test battery, the best implementation of psychological readiness testing, and methods for the biological assessment of healing and recovery.
Acute peritoneal dialysis in the treatment of COVID-19-related acute kidney injury

Abstract The kidney is not typically the main target of severe acute respiratory syndrome coronavirus 2, but surprisingly, acute kidney injury (AKI) may occur in 4–23% of cases, whereas the dialysis management of AKI from coronavirus 2019 has not gained much attention. The severity of the pandemic has resulted in significant shortages in medical supplies, including respirators, ventilators and personal protective equipment. Peritoneal dialysis (PD) remains available and has been used in clinical practice for AKI for >70 years; however, it has been used on only a limited basis and therefore experience and knowledge of its use has gradually vanished, leaving a considerable gap. The turning point came in 2007, with a series of sequential publications providing solid evidence that PD is a viable option. As there was an availability constraint and a capacity limit of equipment/supplies in many countries, hemodialysis and convective therapies became alternatives. However, even these therapies are not available in many countries and their capacity is being pushed to the limit in many cities. Evidence-based PD experience lends support for the use of PD now.
Bioavailability and interactions with other micronutrients of three dietary iron sources in Atlantic salmon, Salmo salar, smolts

Atlantic salmon, Salmo salar L. , with mean initial weight of 60 g were fed a diet based on cod muscle meal supplemented with elemental iron, iron sulphate or haem-bound iron in concentrations of 0, 25, 50, 100, 500 and 1500 mg iron kg ‐1 for 8 weeks. No significant differences in growth or mortality were found, except in fish fed 1500 mg haem iron kg ‐1 , which showed reduced growth. In fish fed diets supplemented with elemental iron below 1500 mg iron kg ‐1 , blood haemoglobin and hepatic iron concentration decreased compared with fish fed the unsupplemented diet. Fish fed diets supplemented with iron sulphate showed increased blood haemoglobin and hepatic iron concentrations between 25 and 100 mg iron kg ‐1 . Fish fed diets supplemented with haem-bound iron showed increased hepatic iron at all dietary iron levels, while blood haemoglobin concentration decreased in the group fed 1500 mg haem iron kg ‐1 . The bioavailability of haem iron relative to sulphate iron was calculated by the slope ratio method to be 239% and 148% using blood haemoglobin and hepatic iron, respectively. Relative bioavailability of elemental iron was zero when dietary supplementation levels were between 25 and 500 mg iron kg ‐1 , while a small part was utilized when 1500 mg elemental iron kg ‐1 was supplemented. Additions of 500 and 1500 mg haem-bound iron kg ‐1 resulted in a complete loss of ascorbic acid in these diets. When these groups were discounted, no significant relationship between hepatic iron and hepatic ascorbic acid was found. There was no significant effect of dietary iron on whole-body manganese concentration and only a weak effect on whole-body zinc concentration. No significant correlations between dietary iron and hepatic copper concentration were found in any of the dietary treatments. This study also showed that the level of inorganic iron supplementation may be reduced by inclusion of 20 g blood meal kg ‐1 in the diet.
Nutrition of the mite Unionicola intermedia, Koenike and its relationship to the inflammatory response induced in its molluscan host Anodonta anatina, L.

Summary The structure and staining properties of the epithelium of the midgut in Unionicola intermedia are described. Intracellular non-specific esterase, acid and alkaline phosphatase are present and confined to vacuoles. Extracellular enzymes are absent. Nymphal and adult stages of U. intermedia feed principally on the haemocytes (= amoebocytes) and mucus of their molluscan host, Anodonta anatina. Feulgen-positive material recorded from the lumen and midgut cells of the mites is believed to be derived from the nucleated haemocytes and to undergo intracellular digestion in the midgut cells. Starved mites show steadily decreasing amounts of Feulgen-positive material in the vacuoles. Haemocytes are present in large numbers below the site of attachment of the mites, their presence represents an inflammatory response, and the mite feeds on the products of this response. Large amounts of bound iron are present in the midgut cells but the origin and role of this is unknown.
Research Article Antibacterial and Antifungal activity of Hydro-Alcoholic Extract of Orthosiphon stamineus benth

The leaves of the plant Orthosiphon stamineus Benth were extracted with different solvents by soxhlet apparatus and screened for antibacterial and antifungal activities. The Hydro-alcoholic extract of the plant Orthosiphon stamineus Benth has showed the presence different active chemical constituents. Antibacterial activity was evaluated using MIC (Agar cup plate) method against gram positive and gram negative bacteria and the antifungal activity is evaluated against the unicellular and multicellular fungi. The 100μg/ml & 200μg/ml of hydro-alcoholic extract was used to find out the antimicrobial properties using Streptomycin, Nystatin (10μg/ml) as a standard against the various strains of gram positive, negative bacteria and fungi. The diameter of inhibition zone was measured in millimeters from the center of disc. The study demonstrates that leaves of Orthosiphon stamineus Benth has showed significant antibacterial and antifungal activities.
S0950268820000229jra 1..9

Circulation of multiple dengue virus (DENV) serotypes in a locale has resulted in individuals becoming infected with mixed serotypes. This research was undertaken to study the clinical presentation, presence of DENV serotypes and serological characteristics of DENV infected patients with co-infections from three Provinces of Sri Lanka where DENV-1 and -2 predominated during the study. A reverse transcription polymerase chain reaction was performed on 1249 patient samples and 301 were positive for DENV (24.1%). DENV-1 was the predominant serotype detected in 137 (45.51%) followed by DENV-2 in 65 (21.59%), DENV-3 in 59 (19.6%) and DENV-4 in 4 (1.32%) patients with mono-infections. Thirty-three patients (10.96%) had DENV co-infections with two or more serotypes. The highest number of co-infections was noted between DENV-1 and DENV-2 (57.57%) suggesting co-infection is driven by the frequency of the circulating serotypes. Platelet counts were significantly higher in DENV co-infected patients although clinical disease severity or white blood cell count, packed cell volume or viraemia were not significantly different in the co-infected compared to the mono-infected patients. Thus co-infection with multiple DENV serotypes does occur but with the exception of improved platelet counts in co-infected patients, there is no evidence that clinical or laboratory measures of disease are altered.
Level of VEGF-A and interleukin 6 in lacrimal fluid of patients with diabetic retinopathy

Diabetic retinopathy (DR) is an ocular manifestation characterized by microvascular complications. The aim of this study was to investigate the relationship between diabetic retinopathy (DR) and two lacrimal angiogenic cytokines, interleukin-6 (Il-6) and vascular endothelial growth factor-A (VEGF-A). A cross-sectional study was conducted at Yaoundé Central Hospital from February,1 to June, 15, 2019. Twenty-six diabetic patients with retinopathy and 55 diabetics without retinopathy were enrolled. Tears were collected with Schirmer strips. The concentrations of VEGF-A and Il-6 were determined by the ELISA test. Statistical analysis was performed using Epi Data version 7.1. 5.2. and GraphPad 5.0. The mean VEGF-A concentration was 163.4 ± 45.1 and 149± 81.8 pg/ml in patients with and without DR respectively (p= 0.292) while tear Il-6 level was 118.5±103 and 119±79.43 pg/ml in patients with and without DR respectively (p= 0.497). The mean tear VEGF level was 165.2 ± 76.10 and 153.50 ± 57.94 pg/mL in patients with and without diabetic maculopathy (DM) respectively (p=0.343) while tear Il-6 level was significantly lower in DM (54.92±32.68) compared to patients without DM (159.10±89.78) (p=0.047). Tear Il-6 level was significantly low in patients with DM. There was no association between the 2 lacrimal biomarkers and diabetic retinopathy. Further analysis is needed for this type of screening in diabetic patients.Keywords: eye, retina, tear, diabetes, biomarker; cytokine.
SARS-CoV-2 virus culture from the upper respiratory tract: Correlation with viral load, subgenomic viral RNA and duration of illness.

In 68 respiratory specimens from a cohort of 35 COVID-19 patients, 32 of them with mild disease, we found SARS coronavirus-2 virus culture and sub-genomic RNA was rarely detectable beyond 8 days after onset of illness although virus RNA by RT-PCR remained detectable for many weeks.
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs

A bioartificial liver device containing human progenitor-like cells attenuates acute damage and inflammation, enhancing liver regeneration in pigs. Beneficial bioartificial livers Bioartificial livers are an attractive option as a bridge to transplant or to promote liver regeneration in cases of acute liver failure. Here, Li et al. tested an extracorporeal bioartificial liver system composed of human liver progenitor-like cells cultured on macroporous scaffolds in a bioreactor that provides alternating air-liquid exposure. Three hours of treatment improved survival, reducing inflammation and promoting native liver regeneration in pigs with drug-induced acute liver failure. Results suggest extracorporeal cell-based bioartificial livers may be a promising treatment for acute liver failure. Clinical advancement of the bioartificial liver is hampered by the lack of expandable human hepatocytes and appropriate bioreactors and carriers to encourage hepatic cells to function during extracorporeal circulation. We have recently developed an efficient approach for derivation of expandable liver progenitor-like cells from human primary hepatocytes (HepLPCs). Here, we generated immortalized and functionally enhanced HepLPCs by introducing FOXA3, a hepatocyte nuclear factor that enables potentially complete hepatic function. When cultured on macroporous carriers in an air-liquid interactive bioartificial liver (Ali-BAL) support device, the integrated cells were alternately exposed to aeration and nutrition and grew to form high-density three-dimensional constructs. This led to highly efficient mass transfer and supported liver functions such as albumin biosynthesis and ammonia detoxification via ureagenesis. In a porcine model of drug overdose–induced acute liver failure (ALF), extracorporeal Ali-BAL treatment for 3 hours prevented hepatic encephalopathy and led to markedly improved survival (83%, n = 6) compared to ALF control (17%, n = 6, P = 0.02) and device-only (no-cell) therapy (0%, n = 6, P = 0.003). The blood ammonia concentrations, as well as the biochemical and coagulation indices, were reduced in Ali-BAL–treated pigs. Ali-BAL treatment attenuated liver damage, ameliorated inflammation, and enhanced liver regeneration in the ALF porcine model and could be considered as a potential therapeutic avenue for patients with ALF.
Equi-protein Replacement of Fishmeal with Leucaena Leaf Protein Concentrate: An Assessment of Performance Characteristics and Muscle Development in the Chicken

A batch of three hundred 7- day old Shaver broiler-chicks was used to assess the equi-protein replacement of fishmeal (FM) protein with Leucaena leaf protein concentrates (LLPC) in broiler-starter mash. Before the feeding trial, the LLPC was analyzed for its protein, amino acids, phytin and tannin contents. Thereafter, five diets were tested. The control (diet 1) had 5% FM protein whose protein was gradually replaced at 25, 50, 75 or 100% with LLPC in diets 2 to 5. Thus the inclusion level of LLPC was 2.24, 4.48, 6.72 or 8.96%, respectively for diets 2 to 5. The chicks were randomly assigned in triplicate of 60 chicks per dietary treatment. The average weight gain (AWG), average feed consumption (AFC) and feed conversion ratio (FCR) of chicks fed the control diet were significantly (P 0.05) different from those fed the LLPC-based diets. In the main, the use of 2.24% LLPC in broiler-chick diets, especially in region with limited supply of fishmeal, in a complementary fashion with other ingredients rich in the limiting essential amino acids (EAAs) is suggested.
Effect of Harvest Time on Yield and Quality of Thymus vulgaris L. Essential Oil in Isfahan Province, Iran

Summary Th yme (Th ymus vulgaris L.) is a perennial sub shrub belonging to family Lamiaceae. Th yme essential oils have been used for many thousands of years, especially in food preservation, pharmaceuticals, alternative medicine and natural therapies. In order to determine the eff ect of harvest time on quality and quantity of essential oil in thyme, this experiment was done in a randomized complete block design with three replications at research station of Islamic Azad University, Khorasgan (Isfahan) during 2010-2011. Plants were harvested in fi ve stages: before blooming, beginning of blooming, 50% blooming, full blooming and fruit set. Th e essential oils obtained from the phenological stages of thyme were analyzed by using GC/MS. Th e results obtained in our study showed that the phenological stages had very signifi cant eff ects (P < 0.01) on essential oil yield and percentage as well as thymol percentage and yield. Th e highest essential oil content of thyme (2.42%) was extracted at the beginning of blooming stage. Analysis and identifi cation of components showed that thymol is the main compound in all samples. Th e highest thymol content of thyme (74.8%) was extracted at the full blooming stage. According to the results of this research, harvesting the thyme at 50% blooming stage have maximum essential oil quality and quantity in Isfahan province.
Whole Genome Sequencing of Four Representatives From the Admixed Population of the United Arab Emirates

Whole genome sequences (WGS) of four nationals of the United Arab Emirates (UAE) at an average coverage of 33X have been completed and described. The selection of suitable subpopulation representatives was informed by a preceding comprehensive population structure analysis. Representatives were chosen based on their central location within the subpopulation on a principal component analysis (PCA) and the degree to which they were admixed. Novel genomic variations among the different subgroups of the UAE population are reported here. Specifically, the WGS analysis identified 4,161,067–4,798,806 variants in the four individual samples, where approximately 80% were single nucleotide polymorphisms (SNPs) and 20% were insertions or deletions (indels). An average of 2.75% was found to be novel variants according to dbSNP (build 151). This is the first report of structural variants (SV) from WGS data from UAE nationals. There were 15,677–20,339 called SVs, of which around 13.5% were novel. The four samples shared 1,399,178 variants, each with distinct variants as follows: 1,085,524 (for the individual denoted as UAE S011), 1,228,559 (UAE S012), 791,072 (UAE S013), and 906,818 (UAE S014). These results show a previously unappreciated population diversity in the region. The synergy of WGS and genotype array data was demonstrated through variant annotation of the former using 2.3 million allele frequencies for the local population derived from the latter technology platform. This novel approach of combining breadth and depth of array and WGS technologies has guided the choice of population genetic representatives and provides complementary, regionalized allele frequency annotation to new genomes comprising millions of loci.
Herpes Simplex Virus DNA Cleavage and Packaging: Association of Multiple Forms of UL15-Encoded Proteins with B Capsids Requires at Least the UL6, UL17, and UL28 Genes

ABSTRACT The UL15 gene of herpes simplex virus (HSV) is one of several genes required for the packaging of viral DNA into intranuclear B capsids to produce C capsids that become enveloped at the inner nuclear membrane. A rabbit antiserum directed against UL15-encoded protein recognized three proteins with apparent M rs of 79,000, 80,000, and 83,000 in highly purified B capsids. The 83,000-M rprotein was detected in type C capsids and comigrated with the product of a UL15 cDNA transcribed and translated in vitro. The 83,000- and 80,000-M r proteins were readily detected in purified virions. Inasmuch as (i) none of these proteins were detectable in capsids purified from cells infected with HSV-1(ΔUL15), a virus lacking an intact UL15 gene, and (ii) corresponding proteins in capsids purified from cells infected with a recombinant virus [HSV-1(R7244), containing a 20-codon tag at the 3′ end of UL15] were decreased in electrophoretic mobility relative to the wild-type proteins, we conclude that the proteins with apparent M rs of 83,000, 80,000, and 79,000 are products of UL15 with identical C termini. The 79,000-, 80,000-, and 83,000-M r proteins remained associated with B capsids in the presence of 0.5 M guanidine HCl and remained detectable in capsids treated with 2.0 M guanidine HCl and lacking proteins associated with the capsid core. These data, therefore, indicate that UL15-encoded proteins are integral components of B capsids. Only the 83,000-M r protein was detected in B capsids purified from cells infected with viruses lacking the UL6, UL17, or UL28 genes, which are required for DNA cleavage and packaging, suggesting that capsid association of the 80,000- and 79,000-M rproteins requires intact cleavage and packaging machinery. These data, therefore, indicate that capsid association of the 80,000- and 79,000-M r UL15-encoded proteins reflects a previously unrecognized step in the DNA cleavage and packaging reaction.