Miracle-dz/UniFAR-FTUs huggingface dataset:
Prior COVID-19 Immunization Does Not Cause IgA- or IgG-Dependent Enhancement of SARS-CoV-2 Infection

Antibody-dependent enhancement (ADE) can increase the rates and severity of infection with various viruses, including coronaviruses, such as MERS. Some in vitro studies on COVID-19 have suggested that prior immunization enhances SARS-CoV-2 infection, but preclinical and clinical studies have demonstrated the contrary. We studied a cohort of COVID-19 patients and a cohort of vaccinated individuals with a heterologous (Moderna/Pfizer) or homologous (Pfizer/Pfizer) vaccination scheme. The dependence on IgG or IgA of ADE of infection was evaluated on the serum samples from these subjects (twenty-six vaccinated individuals and twenty-one PCR-positive SARS-CoV-2-infected patients) using an in vitro model with CD16- or CD89-expressing cells and the Delta (B.1.617.2 lineage) and Omicron (B.1.1.529 lineage) variants of SARS-CoV-2. Sera from COVID-19 patients did not show ADE of infection with any of the tested viral variants. Some serum samples from vaccinated individuals displayed a mild IgA-ADE effect with Omicron after the second dose of the vaccine, but this effect was abolished after the completion of the full vaccination scheme. In this study, FcγRIIIa- and FcαRI-dependent ADE of SARS-CoV-2 infection after prior immunization, which might increase the risk of severe disease in a second natural infection, was not observed.
Towards Home-Based Diabetic Foot Ulcer Monitoring: A Systematic Review

It is considered that 1 in 10 adults worldwide have diabetes. Diabetic foot ulcers are some of the most common complications of diabetes, and they are associated with a high risk of lower-limb amputation and, as a result, reduced life expectancy. Timely detection and periodic ulcer monitoring can considerably decrease amputation rates. Recent research has demonstrated that computer vision can be used to identify foot ulcers and perform non-contact telemetry by using ulcer and tissue area segmentation. However, the applications are limited to controlled lighting conditions, and expert knowledge is required for dataset annotation. This paper reviews the latest publications on the use of artificial intelligence for ulcer area detection and segmentation. The PRISMA methodology was used to search for and select articles, and the selected articles were reviewed to collect quantitative and qualitative data. Qualitative data were used to describe the methodologies used in individual studies, while quantitative data were used for generalization in terms of dataset preparation and feature extraction. Publicly available datasets were accounted for, and methods for preprocessing, augmentation, and feature extraction were evaluated. It was concluded that public datasets can be used to form a bigger, more diverse datasets, and the prospects of wider image preprocessing and the adoption of augmentation require further research.
Healthcare Professionals’ Knowledge about Pediatric Chronic Pain: A Systematic Review

Pediatric chronic pain is a common public health problem with a high prevalence among children and adolescents. The aim of this study was to review the current knowledge of health professionals on pediatric chronic pain between 15–30% among children and adolescents. However, since this is an underdiagnosed condition, it is inadequately treated by health professionals. To this aim, a systematic review was carried out based on a search of the electronic literature databases (PubMed and Web of Science), resulting in 14 articles that met the inclusion criteria. The analysis of these articles seems to show a certain degree of heterogeneity in the surveyed professionals about the awareness of this concept, especially regarding its etiology, assessment, and management. In addition, the extent of knowledge of the health professionals seems to be insufficient regarding these aspects of pediatric chronic pain. Hence, the knowledge of the health professionals is unrelated to recent research that identifies central hyperexcitability as the primary factor affecting the onset, persistence, and management of pediatric chronic pain.
A General Methodology for the Analysis of Capture-Recapture Experiments in Open Populations

We trace the development of a likelihood function representation for the open-population capture-recapture (Jolly-Seber) experiment. We find that the modelling of the birth process in the general model is not consistent with the reduced death-only model and that all formulations to date lead to difficulties in imposing constraints upon the parameters of the birth process. We propose a generalisation to the usual Jolly-Seber representation that models births using a multinomial distribution from a super-population. We show how this leads to simplifications in the numerical optimization of the likelihood and how constraints upon the parameters of the model can now be easily imposed. We show how covariate models using auxiliary variables such as sampling effort or weather conditions to explain capture or survival rates can also be easily added. We also show how this model can be generalised to more than one group of animals. Finally a numerical example is provided which fits a class of models where the capture probabilities, survival probabilities and birth probabilities can each vary over time or among groups or both. This permits sequential model fitting within a comprehensive model framework; an approach akin to that of Lebreton et al (Ecological Monographs, 62, 67-118).
Synergistic Effects of Combined Concurrent Training and Eri-Polyunsaturated Fatty Acid Supplementation on Bone Mineral Density, Muscle Strength, and Inflammation

Obesity has been associated with lower bone mineral density (BMD) and rapid muscle weakness. Regular exercise and polyunsaturated fatty acid (PUFA) consumption have been recognized as nonpharmaceutical approaches to increase BMD and ameliorate muscle weakness. This study aimed to determine the effects of concurrent training (CCT) and Eri-PUFA supplementation on BMD, muscular strength, and inflammation in obese adults. A total of 33 obese participants were randomly assigned to one of three groups (n=11 per group): (1) a placebo group; (2) an Eri-PUFA ingestion group (ERI); or (3) a CCT and Eri-PUFA ingestion group (CCT+ERI). The ERI and CCT+ERI groups received approximately 2.5 g of linolenic acid per day from Eri silkworm pupae. The exercise program included aerobic and resistance exercises performed under supervision three times per week for 8 weeks. Before and after the 8-week intervention, BMD, muscular strength, and inflammatory markers were measured. Only the CCT+ERI group showed a significant increase in lumbar spine BMD (5.1%, P<0.01) and upper-body muscle strength (16.9%, P<0.01) after the intervention, with differences between the groups. After the intervention, both the ERI and CCT+ERI groups showed a significant decrease in the monocyte-to-lymphocyte ratio (−25%, P<0.01 and −21.4%, P<0.05, respectively) and tumor necrosis factor-alpha (−21.6%, P<0.05 and −19.4%, P<0.05, respectively). These findings demonstrate that combining CCT and Eri-PUFA supplementation increases BMD and upper-body muscular strength and decreases inflammation. Although Eri-PUFA consumption did not affect BMD or muscle strength directly, it may have an additive effect on BMD by reducing inflammation.
Intake, Growth and Carcass Traits of Steers Offered Grass Silage and Concentrates Based on Contrasting Cereal Grain Types Supplemented with Field Beans, Peas or Maize By-Products

Simple Summary In Europe, there is increasing interest in exploiting alternative indigenous energy and protein feedstuffs to increase self-sufficiency and sustainability. There is relatively little research information on oats or, field beans or peas as feed ingredients for beef cattle, particularly when used as supplements to grass silage. In terms of reducing nitrogen excretion and feed costs, it is also important to assess if protein supplements can be excluded from concentrate rations without compromising animal performance. This study found that the feeding value of rolled barley was similar to rolled oats and maize meal, and flaked beans and peas were similar to maize gluten feed and maize dried distillers grains when included in the supplementary concentrate to beef cattle offered grass silage. Excluding protein ingredients from a cereal-based concentrate did not affect animal performance and reduced nitrogen excretion. Abstract The study objective was to determine intake and performance of beef cattle individually offered perennial ryegrass-dominant grass silage ad libitum supplemented with 4 kg dry matter daily of, rolled barley or maize meal-based concentrate rations containing supplements of flaked field beans, flaked peas, maize dried distillers grains (MDD) or maize gluten feed (MGF) for 110 days (Experiment 1), rolled barley or rolled oats with or without supplements of flaked field beans or flaked peas for 146 days (Experiment 2), and to quantify the nitrogen balance of diets similar to those offered in Experiment 2 (Experiment 3). The protein supplements were formulated to have similar crude protein concentrations. Cereal type or protein source did not affect intake, growth, feed efficiency and carcass traits in Experiment 1 or 2. Inclusion of a legume protein supplement with barley or oats had no effect on intake or growth performance (Experiment 2), whereas their exclusion decreased nitrogen intake, plasma urea concentrations and urinary and total nitrogen excretion (Experiment 3). The feeding value of barley was similar to oats and maize meal, and flaked beans and peas were similar to MGF and MDD, as supplements to grass silage. Excluding protein ingredients from a cereal-based concentrate did not affect animal performance and reduced nitrogen excretion.
Eulerian-Eulerian CFD Model for the Sedimentation of Spherical Particles in Suspension with High Particle Concentrations

Abstract A computational study of the process of sedimentation of spherical particles in suspensions with high particle concentrations has been performed with the two-fluid Eulerian approach. The solid stress induction in the dispersed phase was modelled through modification of the suspension viscosity. Convectional flow patterns were found and studied during the simulations. The presence of these patterns, which are also observed experimentally, makes the sedimentation process dependent on the rheological behaviour of the suspension. The results of the simulation were validated with experimental results. The present paper shows that Eulerian-Eulerian simulations can account for some of the detailed processes taking place in a settling suspension of particles.
High Glucose Activates Prolyl Hydroxylases and Disrupts HIF-α Signaling via the P53/TIGAR Pathway in Cardiomyocyte

The induction of hypoxia tolerance has emerged as a novel therapeutic strategy for the treatment of ischemic diseases. The disruption of hypoxic signaling by hyperglycemia has been shown to contribute to diabetic cardiomyopathy. In this study, we explored the potential molecular mechanisms by which high glucose (HG) impairs hypoxia-inducible factor-α (HIF-α) signaling in cardiomyocytes. The exposure of H9c2 cell lines to HG resulted in time- and concentration-dependent decreases in HIF-1α and HIF-2α expression together with an increase in prolyl hydroxylase-1,2 (PHD1 and PHD2) expression, the main regulators of HIF-α destabilization in the heart. The exposure of H9c2 cells to normal glucose (5.5 mM) and high glucose (15, 30, and 45 mM) led to dose-dependent increases in p53 and TIGAR and a decrease in SIRT3 expression. The pretreatment of H9c2 with p53 siRNA to knockdown p53 attenuated PHD1 and PHD2 expression, thus significantly enhancing HIF-1α and HIF-2α expression in H9c2 cells under HG conditions. Interestingly, pretreatment with p53 siRNA altered H9c2 cell metabolism by reducing oxygen consumption rate and increasing glycolysis. Similarly, pretreatment with TIGAR siRNA blunted HG-induced PHD1 and PHD2 expression. This was accompanied by an increase in HIF-1α and HIF-2α expression with a reduction in oxygen consumption rate in H9c2 cells. Furthermore, pretreatment with adenovirus-SIRT3 (Ad-SIRT3) significantly reduced the HG-induced expression of p53 and PHDs and increased HIF-1α levels in H9c2 cells. Ad-SIRT3 treatment also regulated PHDs-HIF-1α levels in the hearts of diabetic db/db mice. Our study revealed a novel role of the HG-induced disruption of PHDs-HIF-α signaling via upregulating p53 and TIGAR expression. Therefore, the p53/TIGAR signaling pathway may be a novel target for diabetic cardiomyopathy.
Dietary Fiber Level Improve Growth Performance, Nutrient Digestibility, Immune and Intestinal Morphology of Broilers from Day 22 to 42

Simple Summary Dietary fiber is considered to be a nutrient concentration diluent and an antinutritional factor affecting poultry performance. As a consequence, commercial diets generally contain 2–3% crude fiber. However, crude fiber is also a functional component of normal digestive organ function. Moderate dietary fiber could promote the development of digestive organs, increase digestive enzyme activity and nutrient digestibility, improve health status and enhance growth performance in poultry. At present, there are few systematic studies on the dietary fiber requirements of broilers in the late feeding stage. Therefore, it is of great practical significance to determine the dietary fiber requirements of broilers. Under the conditions of this experiment, a diet with 7–9% crude fiber may promote growth performance by improving the nutrient digestibility, immunity and intestinal morphology of broilers from day 22 to 42. Abstract There are few systematic studies on the dietary fiber requirements of broilers in the late feeding stage, and there are not enough data to support this hypothesis. This experiment was conducted to examine the effects of dietary fiber level on growth performance, nutrient digestibility, immune function and intestinal morphology of broilers from day 22 to 42. A total of 480 one-day-old Arbor Acres broilers with half male and half female were randomly allocated into four groups, with eight replicates in each group and fifteen chickens in each replicate. The experimental period was 42 days. All broilers were fed a basal diet from 1 to 21 days. During the 22–42 day period, the four experimental groups were fed diets with soybean hulls as the fiber source, and crude fiber (CF) levels were 2%, 5%, 8% and 11%, respectively. The results showed that during the 29–42 day period, the average daily feed intake (ADFI) of broilers was higher in the 5% CF and 8% CF groups (p < 0.05), and during the 29–35 day period, the average daily gain (ADG) of broilers was higher and the ratio of feed and gain (F/G) of broilers was lower in the 5% CF and 8% CF groups (p < 0.05). The digestibility of crude protein (CP), ether extract (EE), CF, acid detergent fiber (ADF) and neutral detergent fiber (NDF) was higher in broilers of the 8% CF group (p < 0.05). The immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) content of the plasma of broilers was higher in the 8% CF group (p < 0.05). The villus height (VH) of the duodenum, jejunum and ileum of broilers was higher, and the crypt depth (CD) was lower in the 8% CF group than that in the 2% CF group (p < 0.05). The ratio of VH and CD (V/C) of the duodenum and jejunum of broilers in the 8% CF group was higher than that in the 2% CF group (p < 0.05). The quadratic regression analysis showed that the optimum dietary CF level was 7–9%. In conclusion, under the conditions of this experiment, a diet of 7–9% CF may promote growth performance by improving the nutrient digestibility, immunity and intestinal morphology of broilers from day 22 to 42.
Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial Cells

Clinical data implicate fluctuations of high levels of plasma glucose in cardiovascular diseases. Endothelial cells (EC) are the first cells of the vessel wall exposed to them. Our aim was to evaluate the effects of oscillating glucose (OG) on EC function and to decipher new molecular mechanisms involved. Cultured human ECs (EA.hy926 line and primary cells) were exposed to OG (5/25 mM alternatively at 3 h), constant HG (25 mM) or physiological concentration (5 mM, NG) for 72 h. Markers of inflammation (Ninj-1, MCP-1, RAGE, TNFR1, NF-kB, and p38 MAPK), oxidative stress (ROS, VPO1, and HO-1), and transendothelial transport proteins (SR-BI, caveolin-1, and VAMP-3) were assessed. Inhibitors of ROS (NAC), NF-kB (Bay 11-7085), and Ninj-1 silencing were used to identify the mechanisms of OG-induced EC dysfunction. The results revealed that OG determined an increased expression of Ninj-1, MCP-1, RAGE, TNFR1, SR-B1, and VAMP-3 andstimulated monocyte adhesion. All of these effects were induced bymechanisms involving ROS production or NF-kB activation. NINJ-1 silencing inhibited the upregulation of caveolin-1 and VAMP-3 induced by OG in EC. In conclusion, OG induces increased inflammatory stress, ROS production, and NF-kB activation and stimulates transendothelial transport. To this end, we propose a novel mechanism linking Ninj-1 up-regulation to increased expression of transendothelial transport proteins.
Immuno-Stimulating Activity of 1,25-Dihydroxyvitamin D in Blood Cells from Five Healthy People and in Blasts from Five Patients with Leukemias and Pre-Leukemic States

(1) Hematological malignancies are characterized by an immortalization, uncontrolled proliferation of blood cells and their differentiation block, followed by the loss of function. The primary goal in the treatment of leukemias is the elimination of rapidly proliferating leukemic cells (named blasts). However, chemotherapy, which removes proliferating blasts, also prevents the remaining immune cells from being activated. Acute leukemias affect elderly people, who are often not fit to survive aggressive chemotherapy. Therefore, there is a need of milder treatment, named differentiation therapy, which might simulate the immune system of the patient. 1,25-Dihydroxyvitamin D, or low-calcemic analogs of this compound, were proposed as supporting therapy in acute leukemias. (2) Bone marrow blasts from patients with hematological malignancies, and leukocytes from healthy volunteers were ex vivo exposed to 1,25-dihydroxyvitamin D, and then their genomes and transcriptomes were investigated. (3) Our analysis indicates that 1,25-dihydroxyvitamin D regulates in blood cells predominantly genes involved in immune response, such as CAMP (cathelicidin antimicrobial peptide), CP (ceruloplasmin), CXCL9 (C-X-C motif chemokine ligand 9), CD14 (CD14 molecule) or VMO1 (vitelline membrane outer layer 1 homolog). This concerns blood cells from healthy people, as well as blasts from patients with hematological malignancies. In addition, in one patient, 1,25-dihydroxyvitamin D significantly downregulated transcription of genes responsible for cell division and immortalization. (4) In conclusion, the data presented in this paper suggest that addition of 1,25-dihydroxyvitamin D to the currently available treatments would stimulate immune system, inhibit proliferation and reduce immortal potential of blasts.
Synthesis, Characterization, and Investigation of Novel Ionic Liquid-Based Tooth Bleaching Gels: A Step towards Safer and Cost-Effective Cosmetic Dentistry

The objective of this study was to synthesize a novel choline hydroxide ionic liquid-based tooth bleaching gel. Ionic liquid-based gels were synthesized and characterized using FTIR along with pH testing. Tooth sample preparation was carried out in line with ISO 28399:2020. The effects of synthesized gels on tooth samples were tested. Tooth samples were stained and grouped into three experimental groups: EAI (22% choline hydroxide gel), EAII (44% choline hydroxide gel), and EB (choline citrate gel) and two control groups: CA (commercial at-home 16% carbamide peroxide gel) and CB (deionized water). The tooth color analysis, which included shade matching with the Vitapan shade guide (n = 2), and digital colorimetric analysis (n = 2) were evaluated. The surface characteristics and hardness were analyzed with 3D optical profilometry, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), and Microhardness testing (n = 3), respectively. The tooth color analysis (Vitapan shade guide) revealed that all the tooth samples treated with synthesized choline citrate gel (EB) showed an A1 shade as compared to the other four groups, giving a range of shades. An analysis of the ΔE values from digital colorimetry; EAI, EAII, CA, and CB showed ΔE values in a range that was clinically perceptible at a glance. However, EB showed the highest value of ΔE. The mean microhardness values for the five groups showed that the effects of three experimental gels i.e., 44% choline hydroxide, 22% choline hydroxide, and choline citrate, on the microhardness of the tooth samples were similar to that of the positive control, which comprised commercial at-home 16% carbamide peroxide gel. SEM with EDX of three tested subgroups was closely related in surface profile, elemental composition, and Ca/P ratio. The roughness average values from optical profilometry of four tested subgroups lie within approximately a similar range, showing a statistically insignificant difference (p > 0.05) between the tested subgroups. The synthesized novel experimental tooth bleaching gels displayed similar tooth bleaching actions without any deleterious effects on the surface characteristics and microhardness of the treated tooth samples when compared with the commercial at-home tooth bleaching gel.
CYCLE TYPES OF LINEAR SUBSTITUTIONS OVER FINITE COMMUTATIVE RINGS

The problem of describing the lengths of the independent cycles in the indicated substitution reduces to the case where the ring and characteristic polynomial of the corresponding matrix are primary. The concept of a distinguished polynomial over a primary (local) ring R is introduced and studied. These polynomials are used to obtain formulas for the cycle types of linear substitutions that generalize known formulas for the case where R is a field. If R is a principal ideal ring, the formulas are practically computable. In the case where R is a Galois ring, there are given a complete description of the linear substitutions of maximal order and an algorithm for enumerating the cycles in such substitutions. Estimates of the exponents of the full linear group over a local ring and its congruence subgroup are given.Bibliography: 17 titles.
Spectral diagnostics with the SDO EVE flare lines

The diagnostic use of the soft X-ray and EUV lines observed with the Solar Dynamics Observatory (SDO) Extreme ultraviolet Variability Experiment (EVE) is discussed. We focus on all the flare lines o bserved in the 80‐640 A range (mainly due to Fexviii‐ Fexxiv), showing their use to measure temperatures, emission measures, densities, and chemical abundances. We discuss their identification at the EVE resolution, by using the latest atomic data, and by assess ing possible sources of blending, taking into account higher-resolution solar spectra. We present observations of four fla res, and study in more detail the gradual phase peak of the 7 March 2012 X5.6 flare. Good agreement between observations and theory is found in most cases, and the best lines for diagnostics are recommended. We found reasonable densities (10 11.2 cm −3 from Fexxi lines), but isothermal temperatures (12 MK) lower than those estimated with GOES. We show that EVE can be used to measure relative elemental abundance, and find photospheric argon /iron and calcium/iron abundances. We also show that lines normally formed in the quiet Sun in the low transition region such as O III are the best to study the impulsive phase.