Miracle-dz/UniFAR-FTUs huggingface dataset:
L noise-induced transitions in gene regulatory networks

Important effects of noise on a one-dimensional gene expression model involving a single gene have recently been discussed. However, few works have been devoted to the transition in two-dimensional models which include the interaction of genes. Therefore, we investigate here, a quantitativetwo-dimensionalmodel (MeKS network) of gene expression dynamics describing the competence development in the B. subtilis under the inﬂuence of L´evy as well as Brownian motions, where noises can do the B. subtilis a favor in nutrient depletion. To analyze the transitions between the vegetative and the competence regions therein, two deterministic quantities, the mean ﬁrst exit time (MFET) and the ﬁrst escape probability (FEP) from a microscopic perspective, as well as their averaged versions from a macroscopic perspective, are applied. The relative contribution factor (RCF), the ratio of non-Gaussian and Gaussian noise strengths, is adopted to implement optimal control in these transitions. Schematic representations indicate that there exists an optimum choice that makes the transition occurring at the highest probability. Additionally, we use a geometric concept, the stochastic basin of attraction, to exhibit a pictorial comprehension about the inﬂuence of the L´evy motion on the basin stability of the competence state.
reverses shoot

37 sativa ) mutant with a distinctly prostrate growth habit led to the discovery of a gene category 39 that participates in the shaping of plant form by gravity. Each so-called LAZY gene includes five 40 short regions of conserved sequence. The importance of each of these regions in the LAZY1 41 gene of Arabidopsis thaliana ( AtLAZY1 ) was tested by mutating each region and measuring how 42 well transgenic expression of the resulting protein variant rescued the large inflorescence 43 branch angle of an atlazy1 mutant. The effect of each alteration on subcellular localization was 44 also determined. Region I was required for AtLAZY1 to reside at the plasma membrane, which is 45 necessary for its function. Mutating region V severely disrupted function without affecting 46 subcellular localization. Regions III and IV could be mutated without large impacts on function 47 or localization. Altering region II with two conservative amino acid substitutions (L92A/I94A) 48 had the profound effect of switching shoot gravity responses from negative (upward bending) 49 to positive (downward bending), resulting in a ‘weeping’ inflorescence phenotype. Mechanical 50 weakness of the stem was ruled out as an explanation for the downward bending. Instead, 51 experiments demonstrated that the L92A/I94A change to AtLAZY1 reversed the auxin gradient 52 normally established across stems by the gravity-sensing mechanism. This discovery opens up 53 new avenues for studying how auxin gradients form across organs and new approaches for 54 engineering plant architecture for agronomic and other practical purposes. 55 visualization of subcellular structures in cells of Arabidopsis high PpeTAC1 promotes the horizontal growth of branches in peach trees and is a member of a functionally conserved gene family found in diverse plants species.
Independent Necroptosis during Embryogenesis - Kinase Caspase-8 Blocks Receptor-Interacting Protein Kinase-1

Caspase-8 (Casp8) suppresses receptor-interacting protein kinase-3 (RIPK3)/mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis, demonstrated by the genetic evidence that deletion of Ripk3 or Mlkl prevented embryonic lethality of Casp8 -deficient mice. However, the detailed mechanisms by which Casp8 deficiency triggers necroptosis during embryonic development remain unclear. In this article, we show that Casp8 deletion caused formation of the RIPK1-RIPK3 necrosome in the yolk sac, leading to vascularization defects, prevented by MLKL and RIPK3 deficiency, or RIPK3 RHIM mutant (RIPK3 V448P), but not by the RIPK1 kinase-dead mutant (RIPK1 K45A). In addition, Ripk1 K45A/K45A Casp8 2 / 2 mice died on embryonic day 14.5, which was delayed to embryonic day 17.5 by ablation of one allele in Ripk1 and was completely rescued by ablation of Mlkl . Our results revealed an in vivo role of RIPK3 RHIM and RIPK1 K45A scaffold-mediated necroptosis in Casp8 deficiency embryonic development and suggested that the Casp8-deficient yolk sac might be implicated in identifying novel regulators as an in vivo necroptotic model. ImmunoHorizons , 2022, 6: 465 – 475. E11.5 or E15.5 embryos were fixed in 4% paraformaldehyde; par-affin-embedded tissue sections were stained with 0.05 m g/ml anti-cleaved Casp3 Ab (Cell Signaling Technology). TUNEL staining was performed using the Roche Kit with Proteinase K digestion. Stained slides were digitized using a Nanozoomer digi-tal slide scanner.
Shedding Kinetics of Infectious Hematopoietic Necrosis Virus (IHNV) in Juvenile Spring- and Fall-Run Chinook Salmon of the Columbia River Basin

Simple Summary When a virus infects a host it reproduces in that host and then sheds from the host in order to find new hosts for more rounds of reproduction. Thus, virus shedding is a critical step in the host-to-host transmission cycles that allow a virus to spread across a landscape and persist over time. In Pacific salmon and trout the virus infectious hematopoietic necrosis virus (IHNV) causes significant disease, with up to 50% mortality in outbreaks in some conservation hatcheries. Chinook salmon have evolved as two distinct life-history types, referred to as spring- and fall Chinook salmon, and they are the most abundant host of IHNV in the Columbia River basin (CRB) of Washington, Oregon, and Idaho. Here we examined the timing and quantity of virus shedding from both spring-run and fall-run CRB Chinook salmon after controlled exposures to three IHNV strains representing different virus subgroups. We observed rapid shedding kinetics with similar timing for two virus strains in both host types. However, spring Chinook salmon shed much more virus from the UC subgroup than fall fish, suggesting that spring Chinook salmon may play a dominant role in the ecology and maintenance of IHNV in the CRB. Abstract This investigation sought to characterize the shedding of infectious hematopoietic necrosis virus (IHNV) in two populations of Columbia River Basin (CRB) Chinook salmon (Oncorhynchus tshawytscha). Juvenile spring- and fall-run Chinook salmon were exposed by immersion to each of three IHN virus strains from the UC, MD, and L subgroups, and then monitored for viral shedding from individual fish for 30 days. Detectable quantities of UC, MD and L IHN virus were shed by a subset of fish from each host population (1–9 out of 10 fish total in each treatment group). Viral shedding kinetics were consistent, with a rapid onset of shedding, peak shedding by 2–3 days, and then a rapid decline to below detectable levels by 7 days’ post-exposure to IHNV. Intraspecies variation was observed as spring Chinook salmon shed more UC virus than fall fish: spring Chinook salmon shed UC virus in greater numbers of fish, with 22-fold higher mean peak shedding magnitude, 33-fold higher mean total virus shed per fish, and 900-fold higher total virus shed per treatment group. The L and MD viruses had comparable shedding at intermediate levels in each host population. All viral shedding occurred well before host mortality began, and shedding magnitude did not correlate with virulence differences. Overall, the greater shedding of UC virus from spring Chinook salmon, combined with low virulence, indicates a uniquely high transmission potential that may explain the predominance of UC viruses in CRB Chinook salmon. This also suggests that spring-run fish may contribute more to the ecology of IHNV in the CRB than fall-run Chinook salmon.
Brain Metastasis Brain and Spinal Cord Tumors

Brain metastases originate from tissues outside the central nervous system (CNS) and spread secondarily to the brain. They are 10 times more common than primary brain tumors, and account for more than half of all clinically diagnosed brain tumors in adults [1]. The incidence of brain metastases seems to be rising as the control of systemic cancer has improved and patient survival has subsequently increased. This chapter looks at the epidemiology of these tumors and their prognostic assessment, with a focus on strategies for treating both the tumors and their associated symptoms through surgery, radiosurgery, radiation therapy, and future treatment modalities.
THE EFFECT OF SERVICE QUALITY ON CUSTOMER SATISFACTION AND REPURCHASE INTENTION ON GOODS DELIVERY SERVICES

: The purpose of this research is to examine the influence of service quality on customer satisfaction and repurchase intention on goods delivery services (JNE). The number of samples taken is 100 JNE consumers. The method used was purposive sampling by collecting data through questionnaires. The analysis technique used is the path analysis and sobel test. Based on the results of the analysis it was found that the quality of service has an effect on customer satisfaction is significantly positive, as well as service quality has a positive and significant effect on repurchase intention, and customer satisfaction has a significant positive repurchase intention. The results also show that customer satisfaction can mediate service quality with repurchase intentions. These results prove that to improve customer satisfaction, companies are expected to maintain service quality so that they create a commitment in repurchase intention.
Prospective, Randomized, Blinded Clinical Trial Comparing PEEK and Allograft Spacers in Patients Undergoing Anterior Cervical Discectomy and Fusion Surgeries

Study Design. Prospective, randomized, blinded clinical trial. Objective. To examine clinical and radiological outcomes in patients undergoing anterior cervical discectomy and fusion (ACDF) surgeries randomized to receive either polyether-ether-ketone (PEEK) or structural bone allografts. Summary of Background Data. The biomechanical qualities as well as osteoconductive, osteogenic, and osteoinductive properties of various graft materials have been previously evaluated. There remain questions, however, as to whether there are any clinical and/or radiographic outcome differences in the selection of interbody graft types for ACDF. Methods. Patients undergoing one- to three-level ACDF with single anterior plate fixation were randomized (1:1 ratio) to receive either cortical allograft or PEEK interbody spacers. Radiographic and clinical outcomes were assessed at 3, 6, 12, and 24 months with an additional postoperative radiographic assessment. Results. A total of 120 patients were enrolled and randomized. Comparing clinical outcomes, no differences in arm or neck pain scores were noted; however, there was a statistically significant (≤0.041) improvement in SF-36 PCS scores for the allograft group at all follow-up time points and a tendency toward lower disability scores. Overall, evidence of radiographic fusion was achieved in 87 (91.6%) patients: five (10.2%) and three (6.5%) patients had pseudoarthrosis (P = 0.72) in the PEEK and allograft groups, respectively. At 24 months’ follow-up time, any cervical or segmental alignment restoration achieved with surgery was lost and no statistically significant changes were detected when all levels of surgery were included. Likewise, there were no statistically significant differences between the groups for anterior or posterior body height measurements at the 24 months’ follow-up. Approximately 20% of patients had anterior and posterior subsidence, all grade 0 regardless of the group assignment. Conclusion. Comparable radiographic outcomes were observed for patients undergoing one- to three-level PEEK versus allograft-assisted ACDF surgeries. Although MCID comparisons suggest that allograft and PEEK-treated patients have similar clinical outcomes, testing that incorporates the magnitude of the change suggests that there may be a statistically significant greater magnitude of improvement for the allograft group patients, but further studies with a larger sample size would be helpful to determine if a true effect exists.
The role of work engagement and work meaningfulness on turnover intention of nurses in Rundu, Namibia

Turnover, in the nursing profession seems to have negative consequences that could jeopardize the mandate of the Ministry of Health to deliver safe and effective care. Globally, the nursing sector is known to have a high turnover intention, resulting in actual turnover. The study investigated the role of work engagement and work meaningfulness on turnover intention of nurses in Rundu, Namibia. A quantitative, correlational research design was used. A convenience sampling technique was used to select the sample, and in the end, n=168 nurses formed the sample of the study. Correlation and regression analysis using SPSS was used to analyze the data. Findings revealed that there is a negative statistically significant relationship between turnover intention and work-engagement (r=-0.173; p=0.026) of nurses in Rundu, Namibia. Findings also revealed that there is no statistically significant relationship between turnover intention and work-meaningfulness (r=-0.126; p=0.103) of nurses in Rundu, Namibia. A positive and statistically significant relationship between work-engagement and work-meaningfulness (r=0.587**; p=<.0001) was confirmed. Additionally, work meaningfulness was statistically found to moderate the relationship between turnover intention and work engagement (F = 43.589; df = 2; P = <.000; R2 = 0.589). Stakeholders concerned (. i.e. Ministry of Health and Social meaningfulness as engaged employees and those who find meaning in their work will have reduced chances of leaving the profession. The profession is facing a shortage of nurses and efforts should be diverted to reducing turnover intention.
A Hubble Space Telescope Lensing Survey of X-Ray Luminous Galaxy Clusters. I. A383

We present an analysis of the mass distribution in the core of A383 (z = 0.188), one of 12 X-ray luminous galaxy clusters at z ~ 0.2 selected for a comprehensive and unbiased study of the mass distribution in massive galaxy clusters. Deep optical imaging performed by the Hubble Space Telescope (HST) reveals a wide variety of gravitationally lensed features in the core of A383, including a giant arc, two radial arcs in the halo of the central cluster galaxy, several multiply imaged arcs, and numerous arclets. Based upon the constraints from the various lensed features, we construct a detailed model of the mass distribution in the central regions of the cluster, taking into account both a cluster-scale potential and perturbations from individual cluster galaxies. Keck spectroscopy of one component of the giant arc identifies it as an image of a star-forming galaxy at z = 1.01 and provides an accurate measurement of the mass of the cluster within the projected radius of the giant arc (65 kpc) of (3.5 ± 0.1) × 1013 M☉. Using the weak shear measured from our HST observations, we extend our mass model to larger scales and determine a mass of (1.8 ± 0.2) × 1014 M☉ within a radius of 250 kpc. On smaller scales we use the radial arcs to probe the shape of the total mass distribution in the cluster core (r ≲ 20 kpc) and find that the density profile is more peaked than a single Navarro, Frenk, & White (NFW) dark matter profile. Our findings imply that the dark matter in A383 may be more steeply peaked than NFW predict and that the cD galaxy measurably perturbs the cluster potential well. The optical and X-ray properties of A383 indicate the presence of a central cooling flow, for which we derive a mass deposition rate of ≳200 M☉ yr-1. We also use the X-ray emission from A383 to obtain independent estimates of the total mass within projected radii of 65 and 250 kpc: (4.0) × 1013 and (1.2 ± 0.5) × 1014 M☉, which are consistent with the lensing measurements.
Dendritic Cells Activation Modulate Myeloid but Not Plasmacytoid Regulatory T Cells high CD25+ Human CD4

Human CD4 (cid:1) CD25 (cid:1) regulatory T cells (Treg) play an essential role in the prevention of autoimmune diseases. However, the mechanisms of immune suppression and the spectrum of cells they target in vivo remain incompletely deﬁned. In particular, although Treg directly suppress conventional T cells in vitro, they have been shown to inhibit the Ag-presenting functions of macrophage- and monocyte-derived dendritic cells (DC). We have now studied the maturation of human blood-derived myeloid DC and plasmacytoid DC activated with TLR ligands in the presence of Treg. Preactivated Treg suppressed strongly TLR-triggered myeloid DC maturation, as judged by the blocking of costimulatory molecule up-regulation and the inhibition of proinﬂammatory cytokines secretion that resulted in poor Ag presentation capacity. Although IL-10 played a prominent role in inhibiting cytokines secretion, suppression of phenotypic maturation required cell-cell contact and was independent of TGF- (cid:2) and CTLA-4. In contrast, the acquisition of maturation markers and production of cytokines by plasmacytoid DC triggered with TLR ligands were insensitive to regulatory T cells. Therefore, human Treg may enlist myeloid, but not plasmacytoid DC for the initiation and the ampliﬁcation of tolerance in vivo by restraining their maturation after TLR stimulation. The Journal of Immunology, 2006, 176: 5293–5298. Treg-conditioned mDC have a reduced ability to trigger naive T
Anxiety After Stroke

Background and Purpose— Anxiety after stroke is common and disabling. Stroke trialists have treated anxiety as a homogenous condition, and intervention studies have followed suit, neglecting the different treatment approaches for phobic and generalized anxiety. Using diagnostic psychiatric interviews, we aimed to report the frequency of phobic and generalized anxiety, phobic avoidance, predictors of anxiety, and patient outcomes at 3 months poststroke/transient ischemic attack. Methods— We followed prospectively a cohort of new diagnosis of stroke/transient ischemic attack at 3 months with a telephone semistructured psychiatric interview, Fear Questionnaire, modified Rankin Scale, EuroQol-5D5L, and Work and Social Adjustment Scale. Results— Anxiety disorder was common (any anxiety disorder, 38 of 175 [22%]). Phobic disorder was the predominant anxiety subtype: phobic disorder only, 18 of 175 (10%); phobic and generalized anxiety disorder, 13 of 175 (7%); and generalized anxiety disorder only, 7 of 175 (4%). Participants with anxiety disorder reported higher level of phobic avoidance across all situations on the Fear Questionnaire. Younger age (per decade increase in odds ratio, 0.64; 95% confidence interval, 0.45–0.91) and having previous anxiety/depression (odds ratio, 4.38; 95% confidence interval, 1.94–9.89) were predictors for anxiety poststroke/transient ischemic attack. Participants with anxiety disorder were more dependent (modified Rankin Scale score 3–5, [anxiety] 55% versus [no anxiety] 29%; P<0.0005), had poorer quality of life on EQ-5D5L, and restricted participation (Work and Social Adjustment Scale: median, interquartile range, [anxiety] 19.5, 10–27 versus [no anxiety] 0, 0–5; P<0.001). Conclusions— Anxiety after stroke/transient ischemic attack is predominantly phobic and is associated with poorer patient outcomes. Trials of anxiety intervention in stroke should consider the different treatment approaches needed for phobic and generalized anxiety.
Memorial plaques in urban space of East-European cities: Case of Kharkiv

The present research deals with study of memorial plaques as one of the most widespread commemoration signs in urban space of East-European cities. Kharkiv was selected as an example because of what it is the second largest city in Ukraine, the industrial, scientific, educational and cultural giant that is currently undergoing severe destruction and damage as a result of hostilities. Main species features of memorial plaques as historical sources as well as local history signs are characterized taking into account the Ukrainian traditions of their establishment and existence. Specific attributes that distinguish these objects from other signs of commemoration are emphasized; the authors’ scheme of their typology is described. Evolution of trends regarding their visual design and approaches to the formulation of devotional texts are observed in a century-old retrospective (from the 1920s to the 2020s). The local pantheon of heroes whose names were immortalized by plaques is analyzed. Points of the topographic distribution of these objects in the city districts are identified. It is shown that plaques can be markers of political and ideological confrontation in crisis times. It is forecasted in what way may evolve a complex of these commemoration signs in the postwar period in Kharkiv.
Molecular detection of staphylococcus aureus enterotoxin genes

In this work, S. aureus isolates from meat and meat products were examined for frequency, profiles of antibiotic susceptibility, and virulence genes (seb, sea, sec, sed, see, and fme A). Staphylococcal food poisoning is one of the most economically important foodborne diseases in the world. S. aureus is a prominent food-borne pathogen and common food contamination across the world. Some S. aureus strains generate staphylococcal enterotoxins (SEs), which can cause staphylococcal food poisoning (SFP). Staphylococcus aureus (S. aureus) is one of the most frequently studied foodborne bacteria due to its high pathogenicity, production of heat-stable enterotoxins, and its continued development of resistance to multiple antibiotics. Despite enterotoxigenic staphylococci being thermally killed, cooked beef products may still contain staphylococcal enterotoxins SEs since these toxins are thermo persistent and cannot be removed by heat processing. Therefore, this investigation into the frequency of MRSA genes in certain fresh meat and canned meat was done to learn more about the disease. A total of 80 random samples, Twenty meat samples from butchers, and sixty samples each of Luncheon, Tune, Chicken pieces, Minced meat, Grilled meat, Hamburger Fresh meat and Sausage from some supermarkets in Hilla city in Iraq.
Modularising design processes of façades in Denmark: re-exploring the use of design structure matrix

ABSTRACT Modularity has shown great potential in the manufacturing industry, reducing order lead time and creating variety with limited resources. In the construction industry, the implementation of modularity has been limited to off-site production (OSP). The construction design process incorporates a substantial number of disciplines and stakeholders. Moreover, in the application OSP, the design phase is critical due to the necessity of freezing the design early in the process. This study explores the opportunities for optimising the design processes for OSP through the application of modularity. Framed by a large general contractor, the research is based on a case study of façade design, which is representative of design processes for OSP. Research is based on a theoretical framing within design management and modularity, combined with empirical material from 20 interviews and a two-hour-long workshop with a cross-functional design team. The findings were that (1) the application of a modularity perspective in design has the advantage of accelerating the execution process, as the workload and coordination are transferred to the design process, which, in turn, requires enhanced design management. (2) The design structure matrix (DSM), an approach for operationalising modularity theory, is a promising tool for planning and scheduling complex design processes. The DSM method successfully enabled the identification of dependencies and interfaces between the crucial cross-organisational design activities that are related to the façade design process. (3) The developed process modules are helpful to visualise and execute the process for both project participants and managers.