Miracle-dz/UniFAR-FTUs huggingface dataset:
QUARK CHEESE PRODUCED WITH KEFIR AND AGAVE INULIN

Quark cheese is a cheese of fresh mass obtained by coagulation of the milk by the action mainly of lactic bacteria obtaining a cheese of refreshing flavor with high acidity, in its commercial production a specific starter culture is used, in the present work this culture was replaced by kefir, a symbiotic system of lactic acid bacteria and yeasts, which is considered a probiotic product. Agave inulin is a soluble fiber considered prebiotic with ability to improve the balance of the intestinal flora. The objective of this work was to develop Quark cheese using kefir as a starter culture and supplemented with agave inulin. Cheeses were produced by fermentation for 24h / 25ºC followed by drainage of the whey, in the supplemented cheese 3% of inulin was added, after whey drainage cheeses were kept in plastic pots under refrigeration temperature until the moment of the analyses. Microbiological evaluations, pH and acidity were performed at 1, 7 and 14 days after manufacture. The results of the physical-chemical analyses remained close to those found in the literature. Cheeses showed high counts of Lactobacilli indicating their probiotic potential, the addition of agave inulin decreased the number of these microorganisms, nevertheless they remained in high counts. It can be verified that the use of kefir in the production of Quark cheese is feasible, mainly in artisanal productions because it is usually inexpensive and easy to maintain, Lactobacilli have remained stable during the storage period.
Connecting ocean heat transport changes from the midlatitudes to the Arctic Ocean

Under greenhouse warming, climate models simulate a weakening of the Atlantic Meridional Overturning Circulation and the associated ocean heat transport at midlatitudes but an increase in the ocean heat transport to the Arctic Ocean. These opposing trends lead to what could appear to be a discrepancy in the reported ocean contribution to Arctic amplification. This study clarifies how ocean heat transport affects Arctic climate under strong greenhouse warming using a set of the 21st century simulations performed within the Coupled Model Intercomparison Project. The results suggest that a future reduction in subpolar ocean heat loss enhances ocean heat transport to the Arctic Ocean, driving an increase in Arctic Ocean heat content and contributing to the intermodel spread in Arctic amplification. The results caution against extrapolating the forced oceanic signal from the midlatitudes to the Arctic.
Clinical Characteristics and Local Histopathological Modulators of Endometriosis and Its Progression

Endometriosis (E) and adenomyosis (A) are associated with a wide spectrum of symptoms and may present various histopathological transformations, such as the presence of hyperplasia, atypia, and malignant transformation occurring under the influence of local inflammatory, vascular and hormonal factors and by the alteration of tumor suppressor proteins and the inhibition of cell apoptosis, with an increased degree of lesion proliferation. Material and methods: This retrospective study included 243 patients from whom tissue with E/A or normal control uterine tissue was harvested and stained by histochemical and classical immunohistochemical staining. We assessed the symptomatology of the patients, the structure of the ectopic epithelium and the presence of neovascularization, hormone receptors, inflammatory cells and oncoproteins involved in lesion development. Atypical areas were analyzed using multiple immunolabeling techniques. Results: The cytokeratin (CK) CK7+/CK20− expression profile was present in E foci and differentiated them from digestive metastases. The neovascularization marker cluster of differentiation (CD) 34+ was increased, especially in areas with malignant transformation of E or A foci. T:CD3+ lymphocytes, B:CD20+ lymphocytes, CD68+ macrophages and tryptase+ mast cells were abundant, especially in cases associated with malignant transformation, being markers of the proinflammatory microenvironment. In addition, we found a significantly increased cell division index (Ki67+), with transformation and inactivation of tumor suppressor genes p53, B-cell lymphoma 2 (BCL-2) and Phosphatase and tensin homolog (PTEN) in areas with E/A-transformed malignancy. Conclusions: Proinflammatory/vascular/hormonal changes trigger E/A progression and the onset of cellular atypia and malignant transformation, exacerbating symptoms, especially local pain and vaginal bleeding. These triggers may represent future therapeutic targets.
Concurrent Validity of Four Activity Monitors in Older Adults

Sedentary behaviour (SB) and physical activity (PA) have been shown to be independent modulators of healthy ageing. We thus investigated the impact of activity monitor placement on the accuracy of detecting SB and PA in older adults, as well as a novel random forest algorithm trained on data from older persons. Four monitor types (ActiGraph wGT3X-BT, ActivPAL3c VT, GENEActiv Original, and DynaPort MM+) were simultaneously worn on five anatomical sites during ten different activities by a sample of twenty older adults (70.0 (12.0) years; 10 women). The results indicated that collecting metabolic equivalent (MET) data for 60 s provided the most representative results, minimising variability. In addition, thigh-worn monitors, including ActivPAL, Random Forest, and Sedentary Sphere—Thigh, exhibited superior performance in classifying SB, with balanced accuracies ≥ 94.2%. Other monitors, such as ActiGraph, DynaPort MM+, and GENEActiv Sedentary Sphere—Wrist, demonstrated lower performance. ActivPAL and GENEActiv Random Forest outperformed other monitors in participant-specific balanced accuracies for SB classification. Only thigh-worn monitors achieved acceptable overall balanced accuracies (≥80.0%) for SB, standing, and medium-to-vigorous PA classifications. In conclusion, it is advisable to position accelerometers on the thigh, collect MET data for ≥60 s, and ideally utilise population-specific trained algorithms.
A GLOBAL WAVE ENERGY RESOURCE ASSESSMENT

This paper presents results from an investigation of global wave energy resources derived from analysis of wave climate predictions generated by the WAVEWATCH-III (NWW3) wind-wave model (Tolman, 2002) spanning the 10 year period from 1997 to 2006. The methodology that was followed to obtain these new results is described in detail. The spatial and temporal variations of the global wave energy resource are presented and described. Several parameters to describe and quantify the temporal variation of wave energy resources are presented and discussed. The new results are also validated through comparisons with energy estimates from buoy data and previous studies.
The Economic Value of Seasonal Forecasts Stochastic Economywide Analysis for East Africa

There is growing interest within the climate change and development community in using seasonal forecast information to reduce the losses to agriculture resulting from climate variability, especially within food-insecure countries. However, forecast systems are expensive to establish and maintain, and therefore gauging the potential economic return to investments in forecast systems is crucial. Most studies that evaluate seasonal forecasts focus on developed countries and/or overlook agriculture’s economywide linkages. Yet forecasts may be more valuable in developing regions such as East Africa, where climate is variable and agriculture has macroeconomic importance. We use computable general equilibrium and process-based crop models to estimate the potential economywide value of national seasonal forecast systems in Kenya, Malawi, Mozambique, Tanzania, and Zambia. Stochastic seasonal simulations produce value distributions for forecasts of varying accuracy and varying levels of farm coverage. A timely and accurate forecast adopted by all farmers generates average regional income gains of US$113 million per year. Gains are much higher during extreme climate events and are generally pro-poor. The forecast value falls when forecast skill and farm coverage decline. National economic and trading structures, including the importance of agricultural exports, are found to be major determinants of forecast value. Economywide approaches are therefore needed to complement farm-level analysis when evaluating forecast systems in low-income agrarian economies.
Development and Characterization of Edible Films Based on Cassava Starch Modified by Corona Treatment

Corona treatment (CT), a surface treatment widely used in the plastic industry, can be used to alter the properties of cassava starch. In the present work, CT was performed on dry granular starch (DS), water-suspended humid granular starch (HS), and gelatinized starch (GS). Different properties and structural characteristics of treated starches were studied. A lowering in pH was generally observed after CT and the rheological properties depended on the starch presentation. A reinforcement of DS and HS samples after CT was deduced from higher viscosity values in flow assays and viscoelastic moduli, but weak gels were obtained when CT was applied to GS. Changes in the A-type polymorphic structure, as well as a drop in relative crystallinity, were produced by CT for DS and HS. Additionally, changes in O-H and C-O-C FTIR bands were observed. Therefore, CT can be applied for starch modification, producing predominantly cross-linking in the DS and de-polymerization in the HS. Casting films made from the modified DS showed higher tensile strength and lower hydrophilicity, solubility, water absorption capacity, and water vapor permeability. Thus, the DS cross-linking induced by CT improved mechanical characteristics and hydrophobicity in edible films, which can be better used as packaging materials.
POTENSI PENGEMBANGAN EKOWISATA BERBASIS MASYARAKAT DI KAWASAN RAWA PENING, KABUPATEN SEMARANG

Community-based ecotourism is a rural development effort through tourism sector, which not only serves the natural tourism resources, but also contributes to the ecological conservation, and the community is the main controller in the development. Rawa Pening is a natural lake in Semarang Regency which is laid in four subdistricts, and started to be developed as an ecotourism region, but still has some problems from the ecological aspect, management aspect, and human resources aspect, so the tourism activities in Rawa Pening region hasn't been developed well. Thus, a research is needed to analyze the community-based ecotourism potentials in that region, and to reach that goal, analysis is done in the tourism's object & attraction aspect, community aspect, management aspect, and the implementation of community empowerment aspect. Method used in data collection are literature study, field observation, interview on the informants determined by purposive sampling, and questionnaire on the respondents determined by random sampling. Method used in data analysis is qualitative descriptive method and quantitative method using statistical description analysis method and scoring method to spatially describe the tourism's object & attraction potentials and also the community potentials. Hopefully, the results of those analysis could be a recommendation and consideration in developing the community-based ecotourism concept in Rawa Pening region.
Raw material variability as archaeological tools: Preliminary results from a geochemical study of the basalt vessel workshop at Iron Age Tel Hazor, Israel

The discovery of a basalt vessel workshop at Tel Hazor, one of the most important Iron Age sites in the Near East, marks a turning point in our understanding of stone artifact production and distribution during the1 st millennium BCE. It offers a rare opportunity to characterize ancient raw material sources, production sites, and study production, trade and distribution systems. The basalt vessel workshop, the only one of its kind in the Levant, produced large quantities of bowl preforms and production waste. To better understand the production and distribution systems behind this specialized production center, in 2011 we initiated a focused geochemical project that concentrated on the products of this unique workshop. We measured the major and trace element composition of 44 unfinished basalt vessels from the workshop and other contexts at Hazor, and can demonstrate that the majority of these objects were derived from one specific, geochemically well-constrained, basaltic rock source. Only a few bowls clearly deviate from this geochemical composition and were produced using raw material from other sources. Thus, we believe that one major quarry existed that supplied the Hazor workshop with the majority of the basaltic raw material. The products from this specific extraction site provide us with a “Hazor reference group” that can be used to test whether or not finished vessels from Hazor and contemporary sites were produced in the Hazor workshop.
Microbiota–Gastric Cancer Interactions and the Potential Influence of Nutritional Therapies

Gastric cancer (GC) is one of the most common causes of cancer deaths, and GC treatments represent a large area of research. Although initially regarded as a sterile organ and unsuitable for microbial communities, the discovery of Helicobacter pylori made us realize that some microbes can colonize the stomach. In recent years, growing interest in gastric bacteria has expanded to the gut microbiota and, more recently, to the oral microbiota. Indeed, the oral–gastric–gut microbiota axis may play a crucial role in maintaining homeostasis, while changes in microbiota composition in GC patients can influence clinical outcomes. On the one hand, the microbiota and its metabolites may significantly influence the progression of GC, while anti-GC treatments such as gastrectomy and chemotherapy may significantly impact the oral–gastric–gut microbiota axis of GC patients. In this context, the role of nutritional therapies, including diet, prebiotics, and probiotics, in treating GC should not be underestimated. Wit this review, we aim to highlight the main role of the gastric, oral, and gut microbiota in GC onset and progression, representing potential future biomarkers for early GC detection and a target for efficient nutritional therapies during the course of GC.
The Draft Genome of Kochia scoparia and the Mechanism of Glyphosate Resistance via Transposon-Mediated EPSPS Tandem Gene Duplication

Increased copy number of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene confers resistance to glyphosate, the world’s most-used herbicide. There are typically three to eight EPSPS copies arranged in tandem in glyphosate-resistant populations of the weed kochia (Kochia scoparia). Here, we report a draft genome assembly from a glyphosate-susceptible kochia individual. Additionally, we assembled the EPSPS locus from a glyphosate-resistant kochia plant by sequencing a kochia bacterial artificial chromosome library. These resources helped reconstruct the history of duplication in the structurally complex EPSPS locus and uncover the genes that are co-duplicated with EPSPS, several of which have a corresponding change in transcription. The comparison between the susceptible and resistant assemblies revealed two dominant repeat types. We discovered a FHY3/FAR1-like mobile genetic element that is associated with the duplicated EPSPS gene copies in the resistant line. We present a hypothetical model based on unequal crossing over that implicates this mobile element as responsible for the origin of the EPSPS gene duplication event and the evolution of herbicide resistance in this system. These findings add to our understanding of stress resistance evolution and provide an example of rapid resistance evolution to high levels of environmental stress.
Secondary Modification of S100B Influences Anti Amyloid-β Aggregation Activity and Alzheimer’s Disease Pathology

Proteinaceous aggregates accumulate in neurodegenerative diseases such as Alzheimer’s Disease (AD), inducing cellular defense mechanisms and altering the redox status. S100 pro-inflammatory cytokines, particularly S100B, are activated during AD, but recent findings reveal an unconventional molecular chaperone role for S100B in hindering Aβ aggregation and toxicity. This suggests a potential protective role for S100B at the onset of Aβ proteotoxicity, occurring in a complex biochemical environment prone to oxidative damage. Herein, we report an investigation in which extracellular oxidative conditions are mimicked to test if the susceptibility of S100B to oxidation influences its protective activities. Resorting to mild oxidation of S100B, we observed methionine oxidation as inferred from mass spectrometry, but no cysteine-mediated crosslinking. Structural analysis showed that the folding, structure, and stability of oxidized S100B were not affected, and nor was its quaternary structure. However, studies on Aβ aggregation kinetics indicated that oxidized S100B was more effective in preventing aggregation, potentially linked to the oxidation of Met residues within the S100:Aβ binding cleft that favors interactions. Using a cell culture model to analyze the S100B functions in a highly oxidative milieu, as in AD, we observed that Aβ toxicity is rescued by the co-administration of oxidized S100B to a greater extent than by S100B. Additionally, results suggest a disrupted positive feedback loop involving S100B which is caused by its oxidation, leading to the downstream regulation of IL-17 and IFN-α2 expression as mediated by S100B.
PHOSPHORUS RUNOFF LOSSES FROM SURFACE-APPLIED BIOSOLIDS AND DAIRY MANURE

Biosolids and manures provide nutrients and organic matter to soils but can also pollute runoff. Phosphorus (P) is the limiting nutrient for eutrophication in most fresh water systems, and states are developing strategies to reduce P losses from cropland amended with manure and biosolids. The water extractable P (WEP) of manures and biosolids has been proposed as a predictor of potential to enrich drainage and surface runoff P. A rainfall simulation study was conducted to investigate the relationship between the percent extractable P (PWEP = [WEP ÷ PT] × 100) and runoff P levels. Five P-sources [fresh dairy manure, anaerobically digested biosolids produced without Fe or Al addition, aerobically digested biosolids (elevated Al), anaerobically digested biosolids (elevated Fe), and composted biosolids (elevated Fe)] were surface applied at 112 kg P ha -1 to runoff trays containing an acid-shale and a calcareous soil, each with high and low soiltest P background levels (four soils in all). Trays were sloped at 3% and subjected to 30-min rainfall events (intensity = 7.1 cm hr -1 ). Two rainfall events were performed, separated by a 48hour rest period between events. Collected runoff was analyzed for total mass, runoff dissolved P (RDP), and total (sediment plus dissolved) runoff P (TRP). For all soil treatments, the trend of RDP (mg L -1 ) consistently followed the pattern: dairy manure >> biosolids without Al or Fe > biosolids with Al >composted biosolids with high Fe ≈ biosolids cake with high Fe. The effect of soil type on RDP was found to be statistically different only for the acid-shale high-P (Mehlich-3 P = 520-ppm) material. Runoff DP for all soils was highly correlated (r 2 > 0.98) with the PWEP of the P-source. Average runoff DP from high-Fe biosolids (both cake and compost) was not statistically different from unamended soil control treatments. Total RP (mg L -1 ) was also significantly greater for dairy manure than for biosolids. As P-based nutrient management is implemented for land application, it is imperative that policies reflect different runoff P potentials of biosolids and manures. Moreover, WEP is a simple test that could be developed for routine use as a measure of P-source availability in the P site indices being developed in several states.
A Canadian Survey of Research on HIV-1 Latency—Where Are We Now and Where Are We Heading?

Worldwide, almost 40 million people are currently living with HIV-1. The implementation of cART inhibits HIV-1 replication and reduces viremia but fails to eliminate HIV-1 from latently infected cells. These cells are considered viral reservoirs from which HIV-1 rebounds if cART is interrupted. Several efforts have been made to identify these cells and their niches. There has been little success in diminishing the pool of latently infected cells, underscoring the urgency to continue efforts to fully understand how HIV-1 establishes and maintains a latent state. Reactivating HIV-1 expression in these cells using latency-reversing agents (LRAs) has been successful, but only in vitro. This review aims to provide a broad view of HIV-1 latency, highlighting Canadian contributions toward these aims. We will summarize the research efforts conducted in Canadian labs to understand the establishment of latently infected cells and how this informs curative strategies, by reviewing how HIV latency is established, which cells are latently infected, what methodologies have been developed to characterize them, how new compounds are discovered and evaluated as potential LRAs, and what clinical trials aim to reverse latency in people living with HIV (PLWH).
More is better! Current issues and challenges for museum audience development: A literature review

The purpose of this conceptual paper is to investigate how research on museum management has been approaching audience development and community engagement in our society – especially in Europe, where the development of democratic participation is proclaimed as a shared commitment. This literature review endeavours to grasp both the state of the art in museum audience research and audience development and the most relevant issues emerging in the scientific debate on this topic in the last 20 years (1995-2015). After analysing the main challenges concerning the innovation of the museum sector, the paper discusses the gaps in visitor studies and museum communication, making suggestions for future research and practice. In the field of museum marketing, this review tries to build a theoretical framework linking changes in audiences, museum role, visitor studies and strategies for visitor involvement, to support cultural institutions in achieving their mission and maximizing value creation.