Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic Surveillance of Five SARS-CoV-2 Clinical Samples in Henan Province Using Nanopore Sequencing

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread and poses a major threat to public health worldwide. The whole genome sequencing plays a crucial role in virus surveillance and evolutionary analysis. In this study, five genome sequences of SARS-CoV-2 were obtained from nasopharyngeal swab samples from Zhengzhou, China. Following RNA extraction and cDNA synthesis, multiplex PCR was performed with two primer pools to produce the overlapped amplicons of ~1,200 bp. The viral genomes were obtained with 96% coverage using nanopore sequencing. Forty-five missense nucleotide mutations were identified; out of these, 5 mutations located at Nsp2, Nsp3, Nsp14, and ORF10 genes occurred with a <0.1% frequency in the global dataset. On the basis of mutation profiles, five genomes were clustered into two sublineages (B.1.617.2 and AY.31) or subclades (21A and 21I). The phylogenetic analysis of viral genomes from several regions of China and Myanmar revealed that five patients had different viral transmission chains. Taken together, we established a nanopore sequencing platform for genetic surveillance of SARS-CoV-2 and identified the variants circulating in Zhengzhou during August 2021. Our study provided crucial support for government policymaking and prevention and control of COVID-19.
X-Ray Imaging of Immiscible Fluid Fingering Patterns in a Natural High Porosity Rock

This paper presents the development of a laboratory scale apparatus and first experimental results on the characterization of fingering patterns of immiscible fluids in a porous rock (Fontainebleau sandstone), using three dimensional full-field measurements from x-ray tomography. The few existing studies that have extended experimental investigation of immiscible fluid flow from 2D to 3D have been primarily interested in the pore scale or performed on idealized porous media. While the heterogeneities inherent to natural rocks are known to play an important role on subsurface fluid flow regimes, a limited number of studies have approached the problem of characterizing the time resolved 3D multiphase flow in these material, at the mesoscale. The series of experiments reported in this paper has been performed at a low viscosity ratio, water invasion into oil as the defending fluid, and different capillary numbers (1.8 orders of magnitude). The results illustrate the qualitative transition in the flow regime, from capillary fingering to viscous fingering. While a full quantitative characterization of geometrical features of fluid fingers will require further technical refinements, a qualitative understanding can be already gathered from the results presented herein.
Characterization of circRNA-Associated-ceRNA Networks Involved in the Pathogenesis of Postoperative Cognitive Dysfunction in Aging Mice

Postoperative cognitive dysfunction (POCD) is a clinical entity associated with declined cognitive function following surgery. It occurs more frequently in elderly patients. Recent studies have shown that circRNA-associated-ceRNA networks, constructed based on interactions between circRNA-miRNA and miRNA-mRNA, provide key insight into the molecular mechanisms underlying the pathogenesis of several neurological diseases. However, the mechanism of POCD remains undetermined. In this study, laparotomies were performed under isoflurane anesthesia on young (2-month-old) and aging (17-month-old) male C57BL/6 mice. The results showed that the aging mice were more likely than the young mice to develop POCD. Subsequently, differentially expressed circRNAs, miRNAs, and mRNAs were characterized by RNA sequencing the hippocampi of young and aging mice under control and surgery conditions. Six circRNAs, 6 miRNAs, and 203 mRNAs were identified to construct the circRNA-associated-ceRNA network for the control condition, while 13 circRNAs, 8 miRNAs, and 189 mRNAs were used for the circRNA-associated-ceRNA network for the surgery condition. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of these two networks revealed that the circRNA-associated-ceRNA networks are involved in POCD pathogenesis though modulating the Wnt and VEGF signaling pathways, as well as neural processes associated with long-term synaptic depression and synaptic transmission. In particular, the mmu-miR-298-5P regulatory pathway identified in this study’s mouse model suggests that mm9_circ_009789- and mm9_circ_004229-associated-ceRNA networks as closely related to the occurrence of POCD through regulating PKC signaling pathway, neural cell apoptosis and glycolipid metabolism pathway. These findings provide possible insight into the role of the circRNA-associated-ceRNA networks, helping to unravel the complexity of the molecular pathogenesis of POCD.
Dysregulated Erythroid Mg2+ Efflux in Type 2 Diabetes

Hyperglycemia is associated with decreased Mg2+ content in red blood cells (RBC), but mechanisms remain unclear. We characterized the regulation of Mg2+ efflux by glucose in ex vivo human RBC. We observed that hemoglobin A1C (HbA1C) values correlated with Na+-dependent Mg2+ efflux (Na+/Mg2+ exchange) and inversely correlated with cellular Mg content. Treatment of cells with 50 mM D-glucose, but not with sorbitol, lowered total cellular Mg (2.2 ± 0.1 to 2.0 ± 0.1 mM, p < 0.01) and enhanced Na+/Mg2+ exchange activity [0.60 ± 0.09 to 1.12 ± 0.09 mmol/1013 cell × h (flux units, FU), p < 0.05]. In contrast, incubation with selective Src family kinase inhibitors PP2 or SU6656 reduced glucose-stimulated exchange activation (p < 0.01). Na+/Mg2+ exchange activity was also higher in RBC from individuals with type 2 diabetes (T2D, 1.19 ± 0.13 FU) than from non-diabetic individuals (0.58 ± 0.05 FU, p < 0.01). Increased Na+/Mg2+ exchange activity in RBC from T2D subjects was associated with lower intracellular Mg content. Similarly increased exchange activity was evident in RBC from the diabetic db/db mouse model as compared to its non-diabetic control (p < 0.03). Extracellular exposure of intact RBC from T2D subjects to recombinant peptidyl-N-glycosidase F (PNGase F) reduced Na+/Mg2+ exchange activity from 0.98 ± 0.14 to 0.59 ± 0.13 FU (p < 0.05) and increased baseline intracellular Mg content (1.8 ± 0.1 mM) to normal values (2.1 ± 0.1 mM, p < 0.05). These data suggest that the reduced RBC Mg content of T2D RBC reflects enhanced RBC Na+/Mg2+ exchange subject to regulation by Src family kinases and by the N-glycosylation state of one or more membrane proteins. The data extend our understanding of dysregulated RBC Mg2+ homeostasis in T2D.
Percutaneous Cannulated Screw Fixation vs. Plating With Minimally Invasive Longitudinal Approach After Closed Reduction for Intra-Articular Tongue-Type Calcaneal Fractures: A Retrospective Cohort Study

Objective Displaced intra-articular tongue-type fractures are often treated with surgical interventions, and there is a lack of consensus regarding the surgical approach. This retrospective cohort study aimed to compare percutaneous cannulated screw (PCS) fixation and plating with a minimally invasive longitudinal approach (MILA) after closed reduction for the treatment of tongue-type calcaneal fractures. Materials and Methods A total of 77 patients with intra-articular tongue-type calcaneal fractures between September 2015 and July 2019 were included in this study. They were randomly allocated into two groups: PCS fixation (n = 32) and MILA (n = 45). The outcome measures included demographic variables, operation time (OT), preoperative time (POT), hospital stay time (HST), blood loss, visual analog scale (VAS) scores, American Orthopaedic Foot and Ankle Society (AOFAS) hindfoot scores, postoperative complications, and imaging parameters. The patients were clinically examined at 1, 3, 6, and 12 months, with a final follow-up period of 27 months. Results No significant differences were observed during the follow-up in calcaneal length, height, Gissane's and Böhler's angles, VAS scores, AOFAS hindfoot scores, or complication rates between the two groups. However, the postoperative VAS scores in the PCS group were significantly lower than those in the MILA group (p < 0.05). Furthermore, the OT, POT, and HST in the PCS group were significantly shorter than those in the MILA group (p < 0.05). Blood loss was lower in the PCS group than those in the other group (p = 0.044). However, postoperative calcaneal widening was significantly smaller in the MILA group than that in the PCS group (p < 0.001). Conclusions After closed reduction for the treatment of tongue-type calcaneal fractures, PCS fixation was superior to MILA in terms of OT, POT, HST, blood loss, pain, and degree of comfort. Meanwhile, MILA has the advantage of restoring the calcaneal width. Under the same rehabilitation program, the two approaches showed similar abilities in maintaining the closed reduction.
TP53 Co-Mutations in Advanced EGFR-Mutated Non–Small Cell Lung Cancer: Prognosis and Therapeutic Strategy for Cancer Therapy

Lung cancer is the leading cause of cancer-related deaths worldwide. As the most prevalent molecular mutation subtypes in non-small cell lung cancer (NSCLC), EGFR-TKIs are currently a standard first-line therapy for targeting the mutated EGFR in advanced NSCLC patients. However, 20-30% of this subset of patients shows primary resistance to EGFR-TKIs. Patients with co-mutations of EGFR and several other genes have a poor response to EGFR-TKIs, whereas the prognostic and predictive significance of EGFR/TP53 co-mutation in NSCLC patients remains controversial. Meanwhile, little is known about how to choose an optimal therapeutic strategy for this subset of patients. Presently, no drugs targeting TP53 mutations are available on the market, and some p53 protein activators are in the early stage of clinical trials. A combination of EGFR-TKIs with antiangiogenic agents or chemotherapy or other agents might be a more appropriate strategy to tackle the problem. In this review, we describe the prognostic and predictive value of EGFR/TP53 co-mutation in NSCLC patients, investigate the mechanisms of this co-mutation affecting the response to EGFR-TKIs, and further explore optimal regimens effectively to prolong the survival time of the NSCLC patients harboring this co-mutation.
Intake of Ultra-Processed Food and Ectopic-, Visceral- and Other Fat Depots: A Cross-Sectional Study

Introduction The purpose of this study was to investigate associations between intake of ultra-processed food (UPF) and liver fat, pancreas fat and visceral adipose tissue (VAT) but also subcutaneous adipose tissue (SAT), VAT/SAT ratio and total fat mass. Materials and Methods Cross-sectional analysis of n = 286 50-year old men and women. Energy percentage (%E) from UPF was calculated from a semi-quantitative food frequency questionnaire. Food items were categorized according to the NOVA-classification system and fat depots were assessed using magnetic resonance imaging (MRI) and bioelectrical impedance analysis (BIA). Associations were analyzed using linear regression, adjusted for sex, education, physical activity, smoking, dietary factors and BMI. Results Mean intake of UPF was 37.8 ± 10.2 %E and the three largest contributors to this were crisp- and wholegrain breads and spreads, indicating overall healthy food choices. Consumption of UPF was associated with higher intake of energy, carbohydrates and fiber and lower intake of protein and polyunsaturated fat but no differences were observed for total fat, saturated fat (SFA), monounsaturated fat, sugar or alcohol between tertiles of UPF. Intake of UPF was positively associated with liver- and pancreas fat, VAT, VAT/SAT and inversely associated with total fat mass in crude models. The association for VAT remained after full adjustment (β = 0.01 (95% CI: 0.002, 0.02), P = 0.02) and was driven by women. Conclusion Energy intake from UPF is not associated with ectopic fat, SAT or total fat after adjustment for multiple confounders in this population having overall healthy food habits. However, a positive association between UPF and VAT was observed which was driven by women.
Application of the Gait Deviation Index to Study Gait Impairment in Adult Population With Spinal Cord Injury: Comparison With the Walking Index for Spinal Cord Injury Levels

The Gait Deviation Index (GDI) is a multivariate measure of overall gait pathology based on 15 gait features derived from three-dimensional (3D) kinematic data. GDI aims at providing a comprehensive, easy to interpret, and clinically meaningful metric of overall gait function. It has been used as an outcome measure to study gait in several conditions: cerebral palsy (CP), post-stroke hemiparetic gait, Duchenne muscular dystrophy, and Parkinson’s disease, among others. Nevertheless, its use in population with Spinal Cord Injury (SCI) has not been studied yet. The aim of the present study was to investigate the applicability of the GDI to SCI through the assessment of the relationship of the GDI with the Walking Index for Spinal Cord Injury (WISCI) II. 3D gait kinematics of 34 patients with incomplete SCI (iSCI) was obtained. Besides, 3D gait kinematics of a sample of 50 healthy volunteers (HV) was also gathered with Codamotion motion capture system. A total of 302 (iSCI) and 446 (HV) strides were collected. GDI was calculated for each stride and grouped for each WISCI II level. HV data were analyzed as an additional set. Normal distribution for each group was assessed with Kolmogorov-Smirnov tests. Afterward, ANOVA tests were performed between each pair of WISCI II levels to identify differences among groups (p < 0.05). The results showed that the GDI was normally distributed across all WISCI II levels in both iSCI and HV groups. Furthermore, our results showed an increasing relationship between the GDI values and WISCI II levels in subjects with iSCI, but only discriminative in WISCI II levels 13, 19, and 20. The index successfully distinguished HV group from all the individuals with iSCI. Findings of this study indicated that the GDI is not an appropriate multivariate walking metric to represent the deviation of gait pattern in adult population with iSCI from a normal gait profile when it is compared with the levels of walking impairment described by the WISCI II. Future work should aim at defining and validating an overall gait index derived from 3D kinematic gait variables appropriate for SCI, additionally taking into account other walking ability outcome measures.
DESAIN DAN KONSTRUKSI PERAHU KATAMARAN FIBERGLASS UNTUK WISATA PANCING

Bahan baku kayu pembuat perahu/kapal semakin langka dan mahal. Informasi tentang cara pembuatan perahu berbahan dasar fiberglass pun masih sangat sedikit yang dipublikasikan dalam bentuk karya ilmiah. Penelitian yang dilaksanakan pada bulan Maret-Juli 2012 ini memberikan alternatif solusi untuk kedua permasalahan tersebut melalui proses desain dan konstruksi perahu katamaran fiberglass berdasarkan rujukan BKI. Pengolahan data dilakukan dengan metode numeric berupa formula-formula naval architecture untuk mendapatkan nilai parameter hidrostatis dari kapal yang diteliti. Analisis data dilakukan dengan membandingkan nilai rasio dimensi kapal. Pembuatan perahu katamaran fiberglass diawali dengan pembuatan desain perahu, pembuatan model perahu, pembuatan cetakan perahu dan pembuatan perahu. Desain perahu katamaran yang dibuat memiliki dimensi utama sebagai berikut: panjang (LOA), lebar (B), dalam (D) dan jarak antar lambung secara berurutan yaitu: 4 meter; 1,9 meter; 0,55 meter dan 1 meter. Adapun kelengkapan perahu katamaran ini antara lain: kursi, tempat umpan, mesin, palka, tempat alat pancing, tempat accu , lampu, tempat peralatan dan ruang reserve buoyancy . Konstruksi perahu katamaran ini dilengkapi dengan gading-gading dan galar sebagai penunjang kekuatan melintang dan memanjang dari perahu. Cadangan daya apung/ reserve buoyancy didesain pada perahu ini untuk memperkecil resiko tenggelam. Kata kunci : perahu, katamaran, fiberglass
“What Not to Buy: How to Identify Books Likely to be Unread Before Purchase.”

This study sought to determine if there is a way to predict, before purchase, books likely to never be used in academic libraries. An unweeded community college library was selected and a random sample of unused books was compared to a second sample of items that had checked out at least three times. Two characteristics associated with non-use were found. The first characteristic was that small holdings in LC Classification ranges were associated with low book use. The second predictor was that the book came from a university or academic publisher. The academic book variable was tested against the holdings of two additional community college libraries, and the pattern still held true. Introduction: Large numbers of books in academic libraries are purchased, processed and shelved but are never read, browsed or touched by the readers they are intended to serve. This problem first received widespread recognition in Kent’s 1979 study of materials use at the University of Pittsburgh Library. Kent found that over a seven-year period, almost 40 percent of the books in that collection were never checked out. This replicated results of a 1961 study of the University of Chicago Library by Fussler and Simon, and similar results at a junior college library, College of the Desert. (Hostrop, 1966) Despite the development of WorldCat, which makes for fairly easy Interlibrary Loan (ILL) and easy access to items in consortia, recent studies demonstrate that the problem of too many unread books persists. A 2012 study of the Asbury Theological Seminary libraries in Kentucky and Florida found that, depending on the campus, only 30 to 37 percent of new monographs purchased between 2003 and 2008 were used. (Danielson, 2012) A 2008 study of seven elite liberal arts colleges (Bates, Bowdoin, Bryn Mawr, Colby, Colorado, Haverford and Swarthmore) revealed that 68 percent of titles in these undergraduate libraries never circulated. 5
Deep Else: A Critical Framework for AI Art

From a small community of pioneering artists who experimented with artificial intelligence (AI) in the 1970s, AI art has expanded, gained visibility, and attained socio-cultural relevance since the second half of the 2010s. Its topics, methodologies, presentational formats, and implications are closely related to a range of disciplines engaged in the research and application of AI. In this paper, I present a comprehensive framework for the critical exploration of AI art. It comprises the context of AI art, its prominent poetic features, major issues, and possible directions. I address the poetic, expressive, and ethical layers of AI art practices within the context of contemporary art, AI research, and related disciplines. I focus on the works that exemplify poetic complexity and manifest the epistemic or political ambiguities indicative of a broader milieu of contemporary culture, AI science/technology, economy, and society. By comparing, acknowledging, and contextualizing both their accomplishments and shortcomings, I outline the prospective strategies to advance the field. The aim of this framework is to expand the existing critical discourse of AI art with new perspectives which can be used to examine the creative attributes of emerging practices and to assess their cultural significance and socio-political impact. It contributes to rethinking and redefining the art/science/technology critique in the age when the arts, together with science and technology, are becoming increasingly responsible for changing ecologies, shaping cultural values, and political normalization.
TRAINING OF EDUCATIONAL SOCIAL SKILLS IN MOTHERS OF PREADOLESCENTS WITH HEARING IMPAIRMENT

A Parental Social Skills Program was conducted with mothers of preadolescents with hearing impairment. Two mothers were included in the Experimental Group (EG) and two in the Control Group (CG). The study evaluated the effects of the Program on the repertoire of behaviors of mothers and children using the Interview Script for Parental Social Skills (IS-PSS) and the Child Behavior Checklist (CBCL). The Jacobson Truax method was applied to analyze data of the RE-HSE-P, achieving reliable changes concerning the increment of positive educational social skills and child social skills for the EG. Data of the CBCL indicated internalizing behavior disorders in the EG and in one participant of CG, and externalizing behavior disorders in one participant of the CG before and after intervention and on the follow-up evaluation (for the EG). It is believed that the development of programs like this may constitute an important aid in auditory rehabilitation.
Understanding Motivations and Engagement Outcomes of Social Media Television Coviewing

In today’s media environment, TV programmers and advertisers must strive ever harder to attract the attention of audiences. Yet what may be even more crucial is engaging audiences in conversations on social media and nourishing stronger relationships. To provide insights into how to improve audience experiences through social media television coviewing (STVC) behaviors, this study investigates audience motivations for using social networking sites (SNSs) while watching sports program (i.e., social media television coviewing— STVC) and examines relationships between identified motivations and key audience engagement outcomes. The results reveal four motivations for STVC behaviors: sports-related interaction seeking, information seeking, convenience seeking, and socializing. Further, results reveal that sports-related interaction seeking, information seeking, and socializing motivations are significant predictors of satisfaction, investment, and commitment to the program. Audience engagement outcomes are not predicted, however, by convenience seeking or by variables pertaining to SNS-use regarding STVC behaviors. TV should their informative content SNSs encourage them close acquaintances their social This study has examined the relationship between the identified motivations and key engagement outcomes such as satisfaction, investment, and commitment. The results show that sport-related interaction seeking, information seeking, and socializing are found to be the significant predictors for all engagement outcomes. Both information and sport-related interaction-seeking motivations reflect audiences’ need to receive sport-related information or guidance and exchange opinions with other audiences or sports professionals. Those motivated by sport-related information and interaction seeking must be those who are highly involved with the program or those who are willing to continue a long-lasting relationship with the program. Thus, it is feasible to suppose the needs of such audience lead to high engagement with the program. to the two predictors, that socializing motives contribute to the key audience Socializing like-minded others, close initial program engagement