Miracle-dz/UniFAR-FTUs huggingface dataset:
A Randomized, Blinded, Prospective Clinical Study Comparing Small-Particle Versus Cohesive Polydensified Matrix Hyaluronic Acid Fillers for the Treatment of Perioral Rhytids.

BACKGROUND Perioral rhytids are a bothersome sign of aging for many patients. While multiple treatments exist, choosing an optimal modality may be difficult taking into consideration that rhytids in this region are fine and the anatomy dynamic. OBJECTIVE To compare the efficacy and safety, and patient satisfaction of a small-particle hyaluronic acid filler with 0.3% lidocaine (SP-HAL, Restylane Silk®) and cohesive polydensified matrix hyaluronic acid filler (CPM-HA, Belotero Balance®) in reducing uperficial perioral rhytids. METHODS The study was double-blinded, and 48 subjects with moderate to severe superficial perioral rhytids were enrolled. Subjects were randomized to receive either CPM-HA in the left perioral region and SPHAL in the right, or vice versa. Rhytid severity was measured by patients using a linear analog scale, and for investigators a validated 5-point scale, for 180 days following treatment. RESULTS Both SP-HAL and CPM-HA achieved a reduction in rhytid severity, and neither treatment group returned to baseline after 180 days. Investigator-reported scores for rhytid severity were significantly better for SP-HAL compared to CPM-HA and remained so at 180 days (p < 0.05). SP-HAL also proved significantly better for reducing rhytids according to patient scores, although this difference occurred between 120 and 180 days only. Adverse events included rash and mild acne for CPM-HA, while SP-HAL was associated with one post-inflammatory nodule and two occurrences of Tyndall effect. CONCLUSION While both SP-HAL and CPM-HA are effective at reducing perioral rhytid severity and have similar safety profiles, SP-HAL possesses a longer duration of effect.
Medicines in Western Sahara Refugee Camps in Tindouf: Prescriptions and self-medication mixing in the drawer

ABSTRACT Problems with access to medicines, either by shortage or excess can be especially relevant in refugee camps. In the present study, we describe the medicines found in a sample of the Western Sahara refugee households, with special emphasis on antimicrobials and their use. All households of the 2nd district of Mahbes daira, in the Smara wilaya of the Sahrawi Refugee Camps in Tindouf (Algeria) were visited during 10 days in April 2019 in order to know which medicines are kept at home. Seventy-six families were included in the study and 269 medicines were found. Eight (10.5%) did not have any medical product at home and the remaining 68 (89.5%) had a median of 3 drugs, ranging from 1 to 13. The most consumed drugs were analgesics (35.7%) and antimicrobials (15.6%). Most medicines had been prescribed, but in the case of antimicrobials, an incomplete consumption pattern was observed in 42.9% of the cases and, more worrying, one-third of them were used to treat viral or non-infectious diseases. This first drug utilization study in the Saharawi population highlighted the importance, not only to ensure appropriate access to medicines in refugee’s camps, but also to watch over their appropriate use.
Soil microbial succession following surface mining is governed primarily by deterministic factors.

Understanding the successional dynamics governing soil microbial community assembly following disturbance can aid in developing remediation strategies for disturbed land. However, the influences shaping microbial communities during succession following soil disturbance remain only partially understood. One example of a severe disturbance to soil is surface mining for natural resources, which displaces communities and changes the physical and chemical soil environment. These changes may alter community composition through selective pressure on microbial taxa (i.e. deterministic processes). Dispersal and ecological drift may also shape communities following disturbance (i.e. stochastic processes). Here, the relative influence of stochastic and deterministic processes on microbial community succession was investigated using a chronosequence of reclaimed surface mines ranging from 2-32 years post-reclamation. Sequencing of bacterial and fungal ribosomal gene amplicons coupled with a linear modeling approach revealed that following mine reclamation, while bacterial communities are modestly influenced by stochastic factors, the influence of deterministic factors was ∼7 × greater. Fungal communities were influenced only by deterministic factors. Soil organic matter, texture, and pH emerged as the most influential on both bacterial and fungal communities. Our results suggest that management of deterministic soil characteristics over a sufficient time period could increase the microbial diversity and productivity of mine soils.
Regional Risk Assessment of a Brazilian Rain Forest Reserve

The objective of this study was to identify subareas inside and near an Atlantic Rain Forest reserve, the Parque Estadual Turístico do Alto Ribeira (PETAR), most likely to be affected by land use in the vicinity of the area. In addition, the study aimed to compare risks per stressor source (agriculture, human settlements and mining) to both epigean (surface) and hypogean (subterranean) aquatic fauna. The methodological approach included the relative vulnerability of endpoints to the stressors (pesticides, metals, nutrients, and particles) and ranking of stressor sources and habitats (epigean and hypogean streams) based on their relative distribution in 14 subareas within the catchment areas of the main rivers that cross PETAR: Pilões, Betari and Iporanga. Four subareas presented high risk for both epigean and hypogean fauna. Three of those areas were located inside the Betari catchment area, where most of the settlements and abandoned lead mines are located. The fourth area was situated in the headwaters of the Pilões River, where agricultural activities are intense. Agriculture and human settlements were the activities most likely to cause impacts on aquatic ecosystems. Uses of risk assessment results include management of the PETAR and communication to stakeholders by the Park Administration.
Variability of the response of 66Listeria monocytogenesandListeria innocuastrains to different growth conditions

Abstract The growth of 58 strains of Listeria monocytogenes and eight strains of Listeria innocua isolated from meat products (68%) and industrial sites (23%), were compared in four conditions of temperature, water activity (a w ) and pH. Temperatures ranged from 10–37°C, pH from 5.6–7.0 and a w from 0.96–1. Growths were performed in a meat broth with an automated turbidimeter (Bioscreen C, Labsystem). Growth curves were fitted using the Gompertz function, and growth parameters were calculated. The differences between strains in lag phase duration were much greater than in growth rate. The greatest differences occurred at 10°C, pH7 and a w 0.96 : lag time values ranged from 4h to 4 days. the Listeria population was separated into five groups, according to the lag time and maximal growth rate values using clustering analysis. The majority of the strains isolated from industrial sites were grouped together and showed faster growth than the others in the four conditions studied. The serotype or the nature of the meat from which the strains were isolated did not influence growth. The variability observed among strains raises questions about the consequences in quantitative risk assessment and about the construction of models in predictive modelling.
A study on clinical effect of Arbidol combined with adjuvant therapy on COVID‐19

This study aims to explore the clinical effect of Arbidol (ARB) combined with adjuvant therapy on patients with coronavirus disease 2019 (COVID‐19). The study included 62 patients with COVID‐19 admitted to the First Hospital of Jiaxing from January to March 2020, and all patients were divided into the test group and the control group according to whether they received ARB during hospitalization. Various indexes in the two groups before and after treatment were observed and recorded, including fever, cough, hypodynamia, nasal obstruction, nasal discharge, diarrhea, C‐reactive protein (CRP), procalcitonin (PCT), blood routine indexes, blood biochemical indexes, time to achieve negative virus nucleic acid, and so on. The fever and cough in the test group were relieved markedly faster than those in the control group (P < .05); there was no obvious difference between the two groups concerning the percentage of patients with abnormal CRP, PCT, blood routine indexes, aspartate aminotransferase, and alanine aminotransferase (P > .05); the time for two consecutive negative nucleic acid tests in the test group were shorter than that in the control group; the hospitalization period of the patients in the test group and control group were (16.5 ± 7.14) days and (18.55 ± 7.52) days, respectively. ARB combined with adjuvant therapy might be able to relieve the fever of COVID‐19 sufferers faster and accelerate the cure time to some degree, hence it's recommended for further research clinically.
Exploration of the molecular characteristics of the tumor–immune interaction and the development of an individualized immune prognostic signature for neuroblastoma

Neuroblastoma (NBL) exists in a complex tumor–immune microenvironment. Immune cell infiltration and tumor–immune molecules play a critical role in tumor development and significantly impact the prognosis of patients. However, the molecular characteristics describing the NBL–immune interaction and their prognostic potential have yet to be investigated systematically. We first employed multiple machine learning algorithms, such as Gene Sets Enrichment Analysis and cell type identification by estimating relative subsets of RNA transcripts, to identify immunophenotypes and immunological characteristics in NBL patient data from public databases and then investigated the prognostic potential and regulatory networks of identified immune‐related genes involved in the NBL–immune interaction. The immunity signature combining nine immunity genes was confirmed as more effective for individual risk stratification and survival outcome prediction in NBL patients than common clinical characteristics (area under the curve [AUC] = 0.819, C‐index = 0.718, p < .001). A mechanistic exploration revealed the regulatory network of molecules involved in the NBL–immune interaction. These immune molecules were also discovered to possess a significant correlation with plasma cell infiltration, MYCN status, and the level of chemokines and macrophage‐related molecules (p < .001). A nomogram was constructed based on the immune signature and clinical characteristics, which showed high potential for prognosis prediction (AUC = 0.856, C‐index = 0.755, p < .001). We systematically elucidated the complex regulatory mechanisms and characteristics of the molecules involved in the NBL–immune interaction and their prognostic potential, which may have important implications for further understanding the molecular mechanism of the NBL–immune interaction and identifying high‐risk NBL patients to guide clinical treatment.
The use of nonhuman primates in the study of bilirubin metabolism and bile secretion

Close similarities exist in the chemical structure of fetal and adult bile pigments and mechanisms for hepatic bilirubin conjugation and transport in human and nonhuman primates. Newborn monkeys, unlike other animals, develop physiologic hyperbilirubinemia and are ideal animal models in which to investigate this developmental human disorder. Aberrations in bile pigment metabolism and compartmentalization which occur in hemolytic, hepatocellular, and obstructive diseases, closely resemble those reported in counterpart syndromes in man. Similarities also exist in the mechanisms responsible for the secretion of canalicular and ductular/ductal bile and the composition of bile. Nonhuman primates offer distinct advantages for comparative studies concerning bile pigment metabolism and the secretion of bile.
Repertoire composition and singing behaviour in two eastern populations of the Hermit Thrush (Catharus guttatus)

Although it is highly recognisable, relatively little is known about the repertoire composition and singing behaviour of the Hermit Thrush (Catharus guttatus). To address this, we recorded spontaneously singing males in two eastern populations (Halifax, NS (n = 11) and Hancock County, ME (n = 7)) and analysed the recordings with respect to repertoire size, basic song type characteristics and song syntax. Males had song type repertoires of 7–12 song types, and no song type sharing between individuals was observed within or between populations. While frequency-related structural characteristics of the song types were the same between the populations, song type duration (especially that of the introductory note) differed significantly. The song types within each repertoire could be categorised into high and low song types based on introductory note frequency, and these song type categories also differed with respect to the amount and distribution of spectral energy. In both populations, males sang with immediate variety, never repeating the same song type consecutively, and preferentially used some song-type to song-type transitions more than others. In addition, we found correlational evidence of changes in singing behaviour (e.g., the relative use of low versus high song types) over the course of the breeding season. We discuss these findings in the context of other songbird research as well as their implications for future work examining how Hermit Thrush males utilise their songs in defending territories and attracting mates.
Recherches sur la biologie des rotifères III. — Fécondité et durée de vie comparées chez les femelles amictiques et mictiques de quelques espèces

La duree de vie et le taux net de reproduction (Ro) sont mesures a 20 °C et avec une alimentation optimale chez 8 souches de Rotiferes Monogonontes Pseudotroques, appartenant a 6 especes differentes : Asplanchna brightwelli, Brachionus calyciflorus, Epiphanes brachionus, Notommata copeus, Polyarthra dolichoptera et Synchaeta pectinata. Les durees de vie et taux net de reproduction respectifs des femelles amictiques et des femelles mictiques fecondees et non fecondees sont compares chez les quatre premieres especes. La duree de vie des femelles amictiques varie de 5 a 12 jours selon les especes ou les souches d'une meme espece, de 5 a 8 jours pour les femelles mictiques non fecondees. A l'exception d'Asplanchna brightwelli, le nombre d'oeufs (femelles) pondus par une femelle amictique — soit de 13 oeufs ♀ -1 chez Polyarthra dolichoptera a plus de 40 oeufs ♀ -1 chez Epiphanes senta — est a peu pres equivalent au nombre d'oeufs (mâles) pondus par une femelle mictique. Le taux net de reproduction depend non seulement de la temperature mais aussi de l'alimentation qui n'a que peu d'influence sur la duree de vie.
Ovary Growth and Maize Kernel Set

Synchronous pollination improves maize (Zea mays L.) kernel set, but the physiological reasons behind this response are yet unclear. We registered ovary fresh weight evolution at three fl oret positions along the ear (base, middle and tip) of plants subjected to natural (NP) or synchronous (SP) pollination during two growing seasons. Synchronous pollination of ears bagged before silking was performed fi ve days after silking (DAS). Ovary weight increased exponentially at all fl oret positions (P ≤ 0.01), but a lag in this trait was detected among those at the base and middle of SP ears. At each sampling date, fl orets along the ear differed (P < 0.01) in ovary weight, but differences were larger for NP than for SP plants. At pollination of each fl oret position, however, the range in ovary weight was smaller in NP ears (ca. 4 to 5 mg ovary −1 ) than in SP ears (4 to 9 mg ovary −1 ). Contrarily, just pollinated ovaries at the tip of the ear of NP plants (i.e. on ca. 4–5 DAS) experienced the high growth rate of those already pollinated at the base (ca. 0.7 mg d −1 for the former and ca. 3.7 mg d −1 for the latter). The range in ovary growth rate along the ear was drastically reduced under synchronous pollination on 5 DAS (ca. 0.55 mg d −1 for the base and ca. 0.47 mg d −1 for the tip). The larger the tip-to-base ratio in ovary growth rate, the larger the number of kernels set per plant (r 2 = 0.94; P = 0.03).
Characterization and in vitro assembly of tick‐borne encephalitis virus C protein

Tick‐borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical–chemical approaches, such as size‐exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid‐like structure with that of dengue C protein.
Landscape of variable domain of heavy‐chain‐only antibody repertoire from alpaca

Heavy‐chain‐only antibodies (HCAbs), which are devoid of light chains, have been found naturally occurring in various species including camelids and cartilaginous fish. Because of their high thermostability, refoldability and capacity for cell permeation, the variable regions of the heavy chain of HCAbs (VHHs) have been widely used in diagnosis, bio‐imaging, food safety and therapeutics. Most immunogenetic and functional studies of HCAbs are based on case studies or a limited number of low‐throughput sequencing data. A complete picture derived from more abundant high‐throughput sequencing (HTS) data can help us gain deeper insights. We cloned and sequenced the full‐length coding region of VHHs in Alpaca (Vicugna pacos) via HTS in this study. A new pipeline was developed to conduct an in‐depth analysis of the HCAb repertoires. Various critical features, including the length distribution of complementarity‐determining region 3 (CDR3), V(D)J usage, VJ pairing, germline‐specific mutation rate and germline‐specific scoring profiles (GSSPs), were systematically characterized. The quantitative data show that V(D)J usage and VHH recombination are highly biased. Interestingly, we found that the average CDR3 length of classical VHHs is longer than that of non‐classical ones, whereas the mutation rates are similar in both kinds of VHHs. Finally, GSSPs were built to quantitatively describe and compare sequences that originate from each VJ pair. Overall, this study presents a comprehensive landscape of the HCAb repertoire, which can provide useful guidance for the modeling of somatic hypermutation and the design of novel functional VHHs or VHH repertoires via evolutionary profiles.
Dispersal patterns in a regional koala population in south-east Queensland

Koala dispersal was investigated as part of a detailed ecological study of a nationally significant koala population located 20 km south-east of Brisbane, Queensland. From 1996 to 2000, 195 koalas from three sites were captured and fitted with radio-collars. A total of 40 koalas (23 males and 17 females) dispersed from these sites. Most (93%) dispersing individuals were 20–36 months of age. Three adult females (more than 36 months old) dispersed and no adult males dispersed during the study. A significantly higher proportion of young males dispersed than females. Dispersal occurred between June and December, with most dispersal of males commencing in July and August and that of females commencing between September and November prior to, and early in, the annual breeding season. The mean straight-line distance between the natal and breeding home ranges for males and females was similar and was measured at 3.5 km (range 1.1–9.7 km) and 3.4 km (range 0.3–10.6 km) respectively. Dispersing males and females tended to successfully disperse south and west of their natal home ranges and were generally unable to successfully disperse to urban areas within the study area, as a high proportion of the mortality of dispersing koalas was associated with attacks by domestic dogs and with collisions with vehicles on roads. Information from other studies indicates that most young koalas disperse from their natal areas. It is likely that the social behaviour and mating systems of koala populations provide mechanisms for young koalas to disperse. The potential role of dispersal in the dynamics of regional koala populations is discussed.
Tunnel technique with connective tissue graft versus coronally advanced flap with enamel matrix derivate for root coverage: 2-year results of an RCT using 3D digital measuring for volumetric comparison of gingival dimensions.

AIM The aim of this randomized clinical trial (RCT) was to compare clinical and volumetric outcomes of tunnel technique (TUN) with subepithelial connective tissue graft (CTG) versus coronally advanced flap (CAF) with enamel matrix derivate (EMD) 2 years after gingival recession (GR) treatment. MATERIALS AND METHODS 23 patients contributed 45 Miller class I or II GR. At baseline and follow-up examinations, study models were collected. Their three-dimensional scans allowed precise computer-assisted measurement of recession depth (REC), complete root coverage (CRC), percentage of root coverage (RC), pointwise (pTHK) and mean areal (aTHK) marginal soft tissue thickness. Clinical examination delivered probing depths (PPD) and height of keratinized tissue (HKT). RESULTS 24 months after surgery, digitally evaluated CRC was present in 60.0% of the TUN+CTG and 0.0% of the CAF+EMD treated sites (p<0.0001), meaning a certain relapse of the gingival margin ragarding both approaches. RC amounted to 94.0% (TUN+CTG) and 57.3% (CAF+EMD) respectively (p<0.0001). REC reduction (RECred) was significantly higher for TUN+CTG (1.81±0.56mm) than for CAF+EMD (0.90±0.45mm) (p<0.0001). pTHK and aTHK values were significantly greater in the TUN+CTG group (1.41±0.35mm and 1.11±0.26 mm) than in the CAF+EMD group (0.78±0.32mm and 0.60±0.26mm) (p<0.0001). Statistical analysis detected positive correlations between THK and both RC and RECred (p<0.001). CONCLUSIONS Two years post-operatively, CTG showed better clinical and volumetric outcomes than EMD. Increased THK values were associated with improved outcomes regarding RC and RECred.