Serum Angiotensin-converting Enzyme (ACE) Activity and Electrolyte Level in Primary Hypertension of South Coastal, Odisha

Serum Angiotensin-converting Enzyme (ACE) Activity and Electrolyte Level in Primary Hypertension of South Coastal, Odisha 1Sanjeev Kumar, 2Pramila K Mishra, 3Nirupama Devi, 4Rasmita K Padhy, 5Sarmistha Swain 1-5Department of Biochemistry, M.K. C.G. Medical College, Berhampur, Odisha, India INtRODuCtION Primary hypertension accounts for more than 90% cases of hypertension. Angiotensin-converting enzyme (ACE) is central component of the renin-angiotensin-aldosterone system, which controls blood pressure by regulating the fluid and electrolyte level in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II, which plays an important role in pathophysiology of essential hypertension. Very less study has been done on serum electrolyte and ACE activity in relation to primary hypertension. AIMS AND ObjECtIvES To measure serum ACE level and serum Na and K level in primary hypertension and to correlate their level with severity and duration of hypertension. MAtERIALS AND MEtHODS This study was carried out in the Department of Biochemistry, M.K. C.G. Medical College, Berhampur, Odisha, India, in collaboration with Medicine Department. Hundred hypertensive patients and 100 ageand sex-matched healthy controls were included in the study. Serum ACE enzyme level was estimated by semi autoanalyzer procuring commercial kit from FAR srl via vermin, 12-37026 pescantina – Verona, Italy. Serum Na/K level was estimated by ion selective electrode. Statistical analysis was done by Statistical Package for the Social Sciences version 20. RESuLtS Mean serum ACE level was 130 ± 32 U/L in hypertensive cases in comparison to 60 ± 34 U/L in controls. The differences of ACE enzyme level were significantly increased in cases (p < 0.01). There was positive correlation between ACE enzyme level with serum electrolytes (r = 0.985 and p ≤ 0.001). Serum ACE and electrolyte level were also well correlating with severity of hypertension (r = 0.872 and p ≤ 0.001) but not with duration of disease. CONCLuSION Cardiovascular event can be minimized in patients with high ACE enzyme level by specifically treating with ACE inhibitor and low salt. Relationship between Gestational Age and Heme Oxygenase-1 Levels in Maternal and Cord blood of Preeclamptics 1A Ghosh, 2R tiwari, 3S Nanda, 4S Kharb 1,2,4Department of Biochemistry, Pt. Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India 3Department of Obstetrics and Gynecology, Pt. Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India ObjECtIvE To study the maternal and fetal aspects of preeclampsia by comparing the concentrations of heme oxygenase-1 in maternal and cord blood venous sample and exploring the effect of gestational age at delivery on the differences in their levels. MAtERIALS AND MEtHODS Fifty pregnant women were selected and grouped as group 1 (control) comprising 25 normotensive women immediately after delivery and group II (study group) comprising ageand sex-matched 25 preeclamptic women. Study samples were drawn (maternal venous blood and umbilical cord blood) and heme oxygenase-1 was analyzed by competitive enzyme-linked immunosorbent assay. RESuLtS There was significant rise in serum heme oxygenase-1 levels in preeclamptic women as compared with normotensive pregnant women (p < 0.001). Cord blood heme oxygenase-1 levels in preeclamptic women were significantly higher than those of