Miracle-dz/UniFAR-FTUs huggingface dataset:
The Macrotheme Review A multidisciplinary journal of global macro trends HOW DO MOTIVATIONAL FACTORS INFLUENCE OVERALL JOB SATISFACTION ? A SURVEY ON EMPLOYEES ACROSS PETROCHEMICAL COMPANIES IN SAUDI ARABIA

The purpose of the study is to explore the view of employees working across petrochemical companies in Saudi Arabia on the intrinsic and extrinsic motivational factors that influence overall job satisfaction. The study uses a survey questionnaire distributed to125 employees from different petrochemical companies in Saudi Arabia. However, only 109 respondents completely returned the questionnaire. The answers of these 109 respondents were used for data analysis. In addition, multivariate regression analysis was also applied to investigate the relationship between those motivational factors and overall job satisfaction. The findings of this study showed that intrinsic motivational factors influence overall job satisfaction more than extrinsic motivational factors. The weighted average results revealed that the intrinsic factors which influenced the overall job satisfaction of employees in petrochemical companies in Saudi Arabia are ability utilization, achievement, leadership attention, independence, and social status. Additionally, it was found that the extrinsic factor which predicted overall job satisfaction is co-workers. The results of regression analysis revealed that ability utilization, achievement, independence and social status are the intrinsic motivational factors that positively significantly influence the employees' overall job satisfaction. In addition, coworkers and compensation are the extrinsic factors that positively significantly influence the overall job satisfaction. The study provides specific inputs for petrochemical companies across Saudi Arabia with regards to which factors they should focus on to achieve the highest overall job satisfaction and preclude the resignation of their employees. In addition, this study is also expected to bridge the literature gap in motivational factors in the petrochemical industry, specifically in Saudi Arabia.
The rs35217482 (T755I) Single-Nucleotide Polymorphism in Aldehyde Oxidase-1 Attenuates Protein Dimer Formation and Reduces the Rates of Phthalazine Metabolism

Aldehyde oxidase-1 (AOX1) is a molybdenum-containing enzyme that catalyzes the oxidation of a range of aldehyde compounds and clinical drugs, including azathioprine and methotrexate. The purpose of this study was to elucidate the effects of single-nucleotide polymorphisms (SNPs) in the coding regions of the human AOX1 gene on protein dimer formation and metabolic activity. Six variants [Q314R (rs58185012), I598N (rs143935618), T755I (rs35217482), A1083G (rs139092129), N1135S (rs55754655), and H1297R (rs3731722)], with allele frequencies greater than 0.01 in 1 or more population, were obtained from the genome aggregation and 1000 Genomes Project databases. Protein expression and dimer formation were evaluated using HEK293T cells expressing the wild-type (WT) or different SNP variants of AOX1. Kinetic analyses of phthalazine oxidation were performed using S9 fractions of HEK293T cells expressing WT or each the different mutant AOX1. Although we detected no significant differences among WT AOX1 and the different variants with respect to total protein expression, native PAGE analysis indicated that one of the SNP variants, T755I, found in East Asian populations, dimerizes less efficiently than the WT AOX1. Kinetic analysis, using phthalazine as a typical substrate, revealed that this mutation contributes to a reduction in the maximal rates of reaction without affecting enzyme affinity for phthalazine. Our observation thus indicates that the T755I variant has significantly negative effects on both the dimer formation and in vitro catalytic activity of AOX1. These findings may provide valuable insights into the mechanisms underlying the interindividual differences in the therapeutic efficacy or toxicity of AOX1 substrate drugs. SIGNIFICANCE STATEMENT The T755l (rs35217482) single-nucleotide polymorphism variant of the aldehyde oxidase-1 (AOX1) protein, which is prominent in East Asian populations, suppresses protein dimer formation, resulting in a reduction in the reaction velocity of phthalazine oxidation to less than half of that of wild-type AOX1.
Were they inoperable? Really?

The MitraClip technique has been increasingly used for correction of mitral valve regurgitation in patients in whom surgical mitral repair is considered contraindicated or very risky, but off label use occurs often. Failure of the procedure, translated into moderate to severe rates of residual or recurrent mitral regurgitation, is observed in up to one‐third of the patients, and surgery has been used to correct it in a number of cases, in what can be called an “operation for the inoperable. ” That is precisely the subtitle of a paper published in this issue of the JOCS by Gerfen and colleagues, who analyse their institutional experience with a series of 17 patients. In this Editorial, I comment on this series and the possible reasons for failure of the MitraClip, and on the indications for reintervention and its constraints, which I hope can contribute to the discussion about “further exploration and refinement of patient selection criteria and identify predictors for MitraClip failure,” as the authors suggest.
An alternative proof of global existence for nonlinear wave equations in an exterior domain

The aim of this article is to present a simpliﬁed proof of a global existence result for systems of nonlinear wave equations in an exterior domain. The novelty of our proof is to avoid completely the scaling operator which would make the argument complicated in the mixed problem, by using new weighted pointwise estimates of a tangential derivative to the light cone.
DDDT_A_253031 2685..2693

Xia Yuan* Hongjun Liu* Yufeng Li 1 Wen Li Huiqian Yu Xia Shen 1Department of Anesthesiology, Shanghai Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People’s Republic of China; 2Research Center, Shanghai Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People’s Republic of China; 3Department of Otorhinolaryngology, Shanghai Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People’s Republic of China
Model update and validation of a mistuned high-pressure compressor blisk

ABSTRACT In order to prepare an advanced 4-stage high-pressure compressor rig test campaign, details regarding both accomplishment and analysis of preliminary experiments are provided in this paper. The superior objective of the research project is to contribute to a reliable but simultaneously less conservative design of future high pressure blade integrated disks (blisk). It is planned to achieve trend-setting advances based on a close combination of both numerical and experimental analyses. The analyses are focused on the second rotor of this research compressor, which is the only one being manufactured as blisk. The comprehensive test program is addressing both surge and forced response analyses e.g. caused by low engine order excitation. Among others the interaction of aeroelastics and blade mistuning is demanding attention in this regard. That is why structural models are needed, allowing for an accurate forced response prediction close to reality. Furthermore, these models are required to support the assessment of blade tip timing (BTT) data gathered in the rig tests and strain gauge (s/g) data as well. To gain the maximum information regarding the correlation between BTT data, s/g-data and pressure gauge data, every blade of the second stage rotor (28 blades) is applied with s/g. However, it is well known that s/g on blades can contribute additional mistuning that had to be considered upon updating structural models. Due to the relevance of mistuning, efforts are made for its accurate experimental determination. Blade-by-blade impact tests according to a patented approach are used for this purpose. From the research point of view, it is most interesting to determine both the effect s/g-instrumentation and assembling the compressor stages on blade frequency mistuning. That is why experimental mistuning tests carried out immediately after manufacturing the blisk are repeated twice, namely, after s/g instrumentation and after assembling. To complete the pre-test program, the pure mechanical damping and modal damping ratios dependent on the ambient pressure are experimentally determined inside a pressure vessel. Subsequently the mistuning data gained before is used for updating subset of nominal system mode (SNM) models. Aerodynamic influence coefficients (AICs) are implemented to take aeroelastic interaction into account for forced response analyses. Within a comparison of different models, it is shown for the fundamental flap mode (1F) that the s/g instrumentation significantly affects the forced response, whereas the impact of assembling the compressor plays a minor role.
Managing Systems of Objectives in the agile Development of Mechatronic Systems by ASD - Agile Systems Design

In the development of mechatronic systems and the associated product services and business models, the system of objectives contains all relevant objectives of various stakeholders, as well as requirements, boundary conditions and interdependencies between objectives. It serves as basis to define the solution area for the technical implementation of products and provides the foundation to validate resulting virtual and physical objects in the process of product development. In particular, the objectives of potential customers and users regarding the product change with an increasing dynamic, which leads to a lack of transparency. Accordingly, the product development process is characterized by constant uncertainties. To be able to counteract these uncertainties, companies are increasingly using agile approaches in order to identify potentially changed objectives at an early stage and to adapt the product along the product development process accordingly. An early validation of prototypes with low functionalities is also carried out under consistent customer integration to identify further objectives early on. As a result, the overall process gains in robustness. One approach that supports the developer throughout the entire development process of mechatronic systems is ASD -Agile Systems Design. It consists of principles, methods and processes of the PGE -Product Gen-generation Engineering and contains a constellation of structuring and flexible elements that can be adapted to the respective development context. To support the process of product development, it is imperative to understand the behavior of systems of objectives in order to be able to derive resulting recommendations for the development. Based on a real development project that has been completed according to ASD, the paper introduces an approach to identify the development of the maturity of the systems of objectives. On this basis, a methodology is presented which supports the agile management of systems of objectives to meet the associated requirements. Thus, a handling of systems of objectives in connection with prototypes generated during the process is presented, which leads to a robust safeguarding of the development direction.
1 in Lung Epithelial Cells MEKK1, ERK, and p90 Ribosomal S6 Kinase Activation and IL-8/CXCL8 Expression via b CCAAT/Enhancer-Binding Protein Thrombin-Induced

Thrombin, a serine protease, is a well-known coagulation factor generated during vascular injury and plays an important role in lung inﬂammation. We previously showed that the c-Src– and Rac/PI3K/Akt-dependent NF- k B pathways are involved in thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells (A549). In this study, we investigated the role of the MEK kinase (MEKK)1/ERK/p90 ribosomal S6 kinase (RSK)1–dependent C/EBP b signaling pathway in thrombin-induced IL-8/ CXCL8 expression. Thrombin-induced IL-8/CXCL8 release and IL-8/CXCL8-luciferase activity were attenuated by small interfering RNA (siRNA) of C/EBP b and by cells transfected with the C/EBP b site mutation of the IL-8/CXCL8 construct. Moreover, thrombin-induced k B-luciferase activity was also inhibited by C/EBP b siRNA. The thrombin-induced increases in IL-8/CXCL8 release and IL-8/CXCL8-luciferase were also inhibited by MEKK1 siRNA, PD98059 (an MEK inhibitor), U0126 (an ERK inhibitor), and RSK1 siRNA. Treatment of cells with thrombin caused an increase in C/EBP b phosphorylation at Thr 235 , C/EBP b -luciferase activity, recruitment of C/EBP b to the IL-8/CXCL8 promoter, and C/EBP b -speciﬁc DNA complex formation. C/EBP b phosphorylation and C/EBP b -luciferase activity were inhibited by MEKK1 siRNA, PD98059, and RSK1 siRNA. Stimulation of cells with thrombin resulted in an increase in RSK1 phosphorylation at Thr 359 /Ser 363 , and this effect was inhibited by MEKK1 siRNA and PD98059. The thrombin-induced increase in ERK activation was inhibited by MEKK1 siRNA. These results imply that thrombin activates the MEKK1/ERK/RSK1 signaling pathway, which in turn initiates C/EBP b activation, recruitment of C/EBP b to the IL-8/CXCL8 promoter, and C/EBP b -speciﬁc DNA complex formation, and ultimately induces IL-8/CXCL8 expression and release in lung epithelial cells. The Journal of Immunology , 2014, 192: 000–000.
A Participatory Design “Method Story”: The Case of Patients Living With Mild Acquired Cognitive Impairments

- This paper presents a story on how patients with Mild Acquired Cognitive Impairment(s) (MACI) could be actively involved in Participatory Design (PD) sessions. A detailed description of what mild acquired cognitive impairments entitles is given, followed by an overview of PD and how it might be relevant in the design of new Information and Communication Technology (ICT) solutions for this user group. The story on how we applied the method is presented as a description and reflection by the authors involved in redesigning the layout of a document in a rehabilitation hospital. The paper aims to attract the attention of PD practitioners to the MACI user group and trigger discussion and questions about PD techniques for patients with MACI.
AKIN bg Contributes to SnRK1 Heterotrimeric Complexes and Interacts with Two Proteins Implicated in Plant Pathogen Resistance through Its KIS/GBD Sequence 1

The sucrose nonfermenting-1 protein kinase (SNF1)/AMP-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. In yeast ( Saccharomyces cerevisiae ) and mammals, the b - and g -noncatalytic subunits are implicated in substrate speciﬁcity and subcellular localization, respectively, and regulation of the kinase activity. The atypical bg -subunit has been previously described in maize ( Zea mays ), presenting at its N-terminal end a sequence related to the KIS (kinase interacting sequence) domain speciﬁc to the b -subunits (Lumbreras et al., 2001). The existence of two components, SNF1-related protein kinase (SnRK1) complexes containing the bg -subunit and one SnRK1 kinase, had been proposed. In this work, we show that, despite its unusual features, the Arabidopsis ( Arabidopsis thaliana ) homolog AKIN bg clearly interacts with AKIN b -subunits in vitro and in vivo, suggesting its involvement in heterotrimeric complexes located in both cytoplasm and nucleus. Unexpectedly, a transcriptional analysis of AKIN bg gene expression highlighted the implication of alternative splicing mechanisms in the regulation of AKIN bg expression. A two-hybrid screen performed with AKIN bg as bait, together with in planta bimolecular ﬂuorescence complementation experiments, suggests the existence of interactions in the cytosol between AKIN bg and two leucine-rich repeats related to pathogen resistance proteins. Interestingly, this interaction occurs through the truncated KIS domain that corresponds exactly to a GBD (glycogen-binding domain) recently described in mammals and yeast. A phylogenetic study suggests that AKIN bg -related proteins are restricted to the plant kingdom. Altogether, these data suggest the existence of plant-speciﬁc SnRK1 trimeric complexes putatively involved in a plant-speciﬁc function such as plant-pathogen interactions. YC-AtHSPRO2. Nicotiana benthamiana plants were grown in the greenhouse under 13 h light, 25 (cid:3) C day temperature, and 17 (cid:3) C night temperature for all the agroinﬁltration experiments. Leaves were inﬁltrated (Rathjen et al., 1999) with an exponential phase culture Agro- bacterium tumefaciens strain C58C1 resuspended to an OD 600 of 0.5 with the inﬁltration medium (10 m M MES, pH 5.6, 10 m M MgCl 2 , 200 m M acetosyr- ingone). For the coinﬁltration, equal volumes of both cultures were mixed before agroinﬁltration. Observations were performed 48 h after inﬁltration. Confocal microscopy was performed on an inverted Leica TCS-SP2-AOBS spec-tral confocal laser scanning microscope. Samples were excited with a 514-nm argon laser (50%) with an emission band of 510 to 550 nm for YFP detection and 640 to 700 nm for chlorophyll autoﬂuorescence. Measurements of ﬂuo- rescence emission spectra werecarried out with an excitation at 488 nm (25%), and emission was detected between 500 and 600 nm at 5-nm intervals.
The effect of leadership on job satisfaction and employee performance (Case study: Geo space Intimatika Ltd. in Jakarta)

Leadership is one of the factors that have an important role in achieving the company's vision and mission. The purpose of the research is to find out the influence of leadership on job satisfaction and employee performance at Geo Space Intimatika Ltd. (GSI). There were three hypotheses tested: 1) The effect of leadership on job satisfaction, 2) The influence of leadership on employee performance, and 3) The effect of job satisfaction on employee performance. The research was conducted at Geo Space Intimatika Ltd. in Jakarta. Sampling is done on a census basis (saturated samples). The data analysis method used is SEM-PLS analysis, with the help of smart PLS software. The results of statistical test analysis obtained that leadership variables have a real (significant) effect on job satisfaction and employee performance at Geo Space Intimatika Ltd. In addition, job satisfaction variables also affect employee performance. Thus it can be concluded that leadership factors greatly affect the company's performance.
An Intraovarian Inhibitory Action of Estrogen on Angrogen Production In vivo1

Subcutaneous administration of estradiol-17$ (I mg) to intact, immature female rats for 3 days significantly decreased ovarian androgen levels from 34 ± 7 pg/mg in oil treated control rats to 13 ± 2 pg/mg ovarian tissue. It inhibited ovarian responsiveness to LH, as measured by ovarian androgen, but not as measured by ovarian progesterone concentrations. In oil treated control animals, LH caused an approximately 5-fold increase in ovarian androgen levels, whereaa ovarian androgen levels were not stimulated at all by LH in the estrogen treated rats. This inhibitory effect on ovarian androgen response was also demonstrable after 6 h of estrogen treatment. Administration of estradiol-l7$ to hypophysectomized immature rats for 3 consecutive days suppressed both ovarian androgen and progesterone levels in response to LH. The cause of the discrepency in the progesterone response between intact and hypophyaectomized rats is not clear. However, with regard to the androgen response, it is unlikely that the inhibitory action of estrogen is indirectly mediated by a suppression of endogenous gonadotropin release, since concomitant administration of exogenous gonadotropins failed to alter the estrogen effect. Instead, a direct action on the ovary is suggested by the demonstration that unilateral ovarian estrogen implants exert an inhibitory effect on LH-induced androgen levels in the ipsilateral. but not contralateral, ovary in vivo. These results suggest that in the ovary there exists a local negative feedback loop for the control of testosterone production, in which elevated ovarian estrogen levels suppress ovarian responsiveness to LH stimulation, thereby lowering testosterone production and subsequently decreasing estrogen biosynthesis.
Laser Therapy and Osteoarthritis Disability: An Update of an Unresolved Topic

Laser therapy, initially reported to be extremely successful in Russia and Eastern Europe has been used for some time with no definitive consensus on its utility. The present review extends the search for research on this topic beyond 1998 when a prior analysis was done. The aim was to examine the support for continuing to research this topic. To arrive at a salient set of conclusions and research recommendations all relevant controlled clinical trials and related basic research published in English-language publications between 1980-2017 were examined. Employing the PUBMED, Scopus, Web of Science, and Academic Search Complete data bases available experimental studies tend to continue to support current findings of post-treatment clinical improvements in symptoms associated with osteoarthritis, including pain, mobility, and function, with few adverse effects. It is concluded that despite prior conclusions that laser therapy is no better than placebo, the current data base affords a much more positive view from a diverse body of related research. Moreover, laser therapy in its various forms can be expected to offer some advantages to the osteoarthritic patient relative to other forms of treatment, or can supplement treatments, thereby raising their effectiveness. Hence the idea that laser therapy may be helpful in postponing joint replacement surgery seems worthy of more attention.
Development of diagnostic method for marine fish pathogens

Rapid detection of bacterial fish pathogens in aquaculture is very important for effective disease control in Turkey which has total production capacity of 240 thousand tons of cultured fish. Conventional microbiological methods are time consuming and lack sensitivities to detect latent marine pathogens. The application of new biotechnologic methods for detection will make a significant impact on the development of such rapid diagnostic approach to aquaculture. In this study, we have used gene based PCR methods to developed rapid diagnostic kit for fast detection of bacterial infection in marine fish. Four different modified oligonucleotid primers (vvhA, aero, gyrB and hsp70) have designed to detect of Vibrio spp . , Aeromonas spp. , Pseudomonas spp. , and Mycobacteria spp. from marine fish. As a result of this study, four different bacterial agents Aeromonas salmonicida , Vibrio anguillarum , Pseudomonas anguilliseptica and Mycobacterium marinum simultaneously has been identified and detected with using 8 primers in same-single tube in very short time.
Impact of Intraoperative Fluid Management on Electrolyte and Acid-Base Variables During Posterior Spinal Fusion in Adolescents

in the electrolyte and buffer concentrations between these in differential in electrolyte and acid-base status during fluid resuscitation. study evaluates these during posterior spinal fusion in Methods: Patients were randomized to receive lactated Ringers (LR), normal saline (NS) or Normosol-R® (NR) during posterior spinal fusion (N=19, 20, and 20, respectively). The specific fluid was used for maintenance fluids as well as fluid replacement of deficits, third space losses, and blood loss. Results: Patients who received NS had a greater base deficit (NS: −2.0 ± 2.2 vs NR −0.6 ± 1.8, p=0.031 or LR: −0.2 ± 1.7, p=0.007) and were more likely to have a ≥2 point change in the base deficit (60% with NS compared to 30% with NR and 47% with LR). Patients receiving NS also had a lower pH (NS: 7.37 ± 0.03 vs NR: 7.39 ± 0.04, p=0.013) and a greater change in pH (NS: −0.03 ± 0.04 vs NR: 0.01 ± 0.06). Conclusion: The use of NS for intraoperative resuscitation during posterior spinal fusion in adolescents resulted in a greater base deficit and a lower pH than the use of LR or NR. Although these changes had limited clinical significance in our patient population, future studies are indicated to further investigate the potential clinical impact of these changes.