Miracle-dz/UniFAR-FTUs huggingface dataset:
Low-Power and Eco-Friendly Temperature Sensor Based on Gelatin Nanocomposite

An environmentally-friendly temperature sensor has been fabricated by using a low-cost water-processable nanocomposite material based on gelatin and graphene. The temperature dependence of the electrochemical properties has been investigated by using cyclic voltammetry, chronopotentiometry and impedance spectroscopy measurements. The simple symmetric device, composed of a sandwich structure between two metal foils and a printable graphene–gelatin blend, exhibits a dependence on the open-circuit voltage in a range between 260 and 310 K. Additionally, at subzero temperature, the device is able to detect the ice/frost formation. The thermally-induced phenomena occur at the electrode/gel interface with a bias current of a few tens of μA. The occurrence of dissociation reactions within the sensor causes limiting-current phenomena in the gelatin electrolyte. A detailed model describing the charge carrier accumulation, the faradaic charge transfer and diffusion processes within the device under the current-controlled has been proposed. In order to increase the cycle stability of the temperature sensor and reduce its voltage drift and offset of the output electrical signal, a driving circuit has been designed. The eco-friendly sensor shows a temperature sensitivity of about −19 mV/K, long-term stability, fast response and low-power consumption in the range of microwatts suitable for environmental monitoring for indoor applications.
Synergistic effects of Garcinia mangostana and Clitoria ternatea extract mixture on antioxidant activities, colour, and anthocyanin stabilities

The present work determined the antioxidant activities, and colour and anthocyanin stabilities of Garcinia mangostana peel (GMP) and Clitoria ternatea flower (CTF) extract mixtures. The plants were extracted at three different ratios of GMP:CTF at three different extractant pH levels (3.0, 3.5, and 4.0). The highest synergistic effects of ORAC, FRAP, ABTS, and DPPH radical scavenging capacity were observed in the mixture of 25% GMP and 75% CTF (25GMP:75CTF) extracted at pH 3.5. Based on the kinetic degradation of anthocyanin, and the L*, C*, and h° values, 25GMP:75CTF extracted at pH 3.5 exhibited a significantly lower rate constant (-k = 2.27) and higher half-life (t1/2 = 5.09 h) over 100°C, thus indicating the most stable mixture ratio and extractant pH condition of those tested. Therefore, the mixture of GMP and CTF at a ratio of 25:75 and an extractant pH of 3.5 produced the most stable extract with a good synergistic effect.
Baseline study on the behaviour of cold-formed steel columns subjected to fire

In this paper is presented a state-of-the-art and future research pathways on cold-formed steel (CFS) columns subjected to fire, the experimental set-upbuilt at the University of Coimbra for testing CFS columns and some preliminary results obtained in the tests, comparing them with the ones existing in the literature.The main objective of the experimental research already carried out is to assess the fire resistance, mechanical behaviour and characterize the failure modes of cold-formed steel columns with restraining thermal elongation in case of fire, develop simplified calculation methods and provide experimental data for future numerical studies.
Stroke Risk in Patients with Gout: A Nationwide Retrospective Cohort Study in Taiwan

Objectives: To estimate stroke risk in Taiwanese patients with gout. Methods: We enrolled patients from the Taiwan National Health Insurance Database, with gout diagnosed from 2000 to 2008, and followed them up until December 2018. This cohort was propensity score-matched according to birth year, sex, the date of diagnosis of gout, comorbidities, and co-medications with individuals without gout (controls) (n = 310,820 in each group). Stroke was defined as the primary diagnosis at discharge after the index date. To evaluate ischemic and hemorrhagic stroke risks, we calculated their incidence, hazard ratio (HR), and two-year moving average incidence rate. Results: The incidence (95% CI) and HR of ischemic stroke were lower in the gout group than in the control group in the first 3 years (incidence: 4.74 [4.60–4.88] vs. 5.17 [5.03–5.32] per 1000 person-years; HR: 0.92 [0.88–0.96]), then became significantly higher than in the control group after 3 years (incidence: 4.10 [4.04–4.16] vs. 3.81 [3.75–3.87] per 1000 person-years; HR: 1.08 [1.05–1.10]). Similarly, the incidence (95% CI) and HR of hemorrhagic stroke was lower in the gout group than in the control group in the first 3 years (incidence: 1.51 [1.43–1.59] vs. 1.70 [1.62–1.79] per 1000 person-years; HR: 0.88 [0.82–0.92]), then became significantly higher than in controls after 3 years (incidence: 1.43 [1.39–1.46] vs. 1.26 [1.22–1.29] per 1000 person-years; HR: 1.14 [1.10–1.18]). Conclusions: In Taiwan, patients with gout had higher risks of ischemic and hemorrhagic stroke after 3 years.
Inflammatory Breast Cancer: The Secretome of HCMV+ Tumor-Associated Macrophages Enhances Proliferation, Invasion, Colony Formation, and Expression of Cancer Stem Cell Markers

Inflammatory breast cancer (IBC) is a highly aggressive phenotype of breast cancer that is characterized by a high incidence early metastasis. We previously reported a significant association of human cytomegalovirus (HCMV) DNA in the carcinoma tissues of IBC patients but not in the adjacent normal tissues. HCMV-infected macrophages serve as “mobile vectors” for spreading and disseminating virus to different organs, and IBC cancer tissues are highly infiltrated by tumor-associated macrophages (TAMs) that enhance IBC progression and promote breast cancer stem cell (BCSC)-like properties. Therefore, there is a need to understand the role of HCMV-infected TAMs in IBC progression. The present study aimed to test the effect of the secretome (cytokines and secreted factors) of TAMs derived from HCMV+ monocytes isolated from IBC specimens on the proliferation, invasion, and BCSC abundance when tested on the IBC cell line SUM149. HCMV+ monocytes were isolated from IBC patients during modified radical mastectomy surgery and tested in vitro for polarization into TAMs using the secretome of SUM149 cells. MTT, clonogenic, invasion, real-time PCR arrays, PathScan Intracellular Signaling array, and cytokine arrays were used to characterize the secretome of HCMV+ TAMs for their effect on the progression of SUM149 cells. The results showed that the secretome of HCMV+ TAMs expressed high levels of IL-6, IL-8, and MCP-1 cytokines compared to HCMV- TAMs. In addition, the secretome of HCMV+ TAMs induced the proliferation, invasion, colony formation, and expression of BCSC-related genes in SUM149 cells compared to mock untreated cells. In addition, the secretome of HCMV+ TAMs activated the phosphorylation of intracellular signaling molecules p-STAT3, p-AMPKα, p-PRAS40, and p-SAPK/JNK in SUM149 cells. In conclusion, this study shows that the secretome of HCMV+ TAMs enhances the proliferation, invasion, colony formation, and BCSC properties by activating the phosphorylation of p-STAT3, p-AMPKα, p-PRAS40, and p-SAPK/JNK intracellular signaling molecules in IBC cells.
Establishment and verification of potential biomarkers for cholangiocarcinoma

Cholangiocarcinoma (CCA) is a malignancy arising from multiple locations along the biliary tree, which is still lacking effective diagnostic biomarkers. The present study aimed to provide a comprehensive differential gene expression profile for the disease. The differentially expressed genes (DEGs) for CCA were explored in-depth using a Gene Expression Omnibus (GEO) dataset, an internal cohort of clinical participants as well as in vitro experiments with the HUCCT1 cell line. Based on the GEO dataset, potential biomarker genes were proposed and the enriched biological processes as well as signaling pathways were further investigated. A protein-protein interaction network of target genes was established. In the clinical specimens, the functions of the primary candidate, FBJ murine osteosarcoma viral oncogene homolog B (FOSB), were evaluated by reverse transcription-quantitative (RT-q)PCR and western blot analysis. A Cell Counting Kit-8 (CCK-8) assay was used for a functional study on FOSB. The results indicated that, compared with non-tumor bile duct tissues, primary CCA samples had 676 differentially expressed genes, including 277 downregulated and 399 upregulated ones. Among these, HBD, FOSB, HBB, ITIH2, FCGBP, MT1JP, PIJR, SLC38A1, COL10A1 and MMP19 were determined to be the most significant DEGs. At the same time, upregulated genes were enriched in ‘vasculature development’ and ‘IL-17 signaling pathways’. Downregulated genes were enriched in ‘extracellular matrix progress’ and ‘glucuronate signaling pathway’. The patients with CCA displayed decreased levels of hemoglobin. Compared with paracancerous tissues, CCA cancerous tissues exhibited increased RNA and protein expression levels of FOSB according to RT-qPCR and western blot analysis, respectively. Furthermore, FOSB expression influenced the proliferation/viability of the CCA cell line HUCCT1. In conclusion, the present study suggested that the FOSB gene may serve as a primary biomarker candidate for CCA, providing a valuable reference for its clinical implementation.
Effect of Psidium guajava L. leaf extract on beef quality at different storage temperatures

Psidium guajava L. , or guava, has been widely reported as having antimicrobial activities against foodborne pathogens. However, the efficacy of P. guajava leaf extract at different storage temperatures has not been extensively explored. Therefore, the present work investigated the effect of antibacterial activity of P. guajava leaf extract on beef quality at different storage temperatures. Disc diffusion assay was performed on selected foodborne pathogens (Bacillus cereus ATCC33019, B. megaterium ATCC14581, B. pumilus ATCC14884, B. subtilis ATCC6633, Escherichia coli ATCC43895, Enterobacter aerogenes ATCC13048, Klebsiella pneumoniae ATCC13773, Pseudomonas aeruginosa ATCC9027, Salmonella Typhimurium ATCC14028, and Staphylococcus aureus ATCC29737) to evaluate the antibacterial activity of the ethanolic extract of P. guajava leaves. The results revealed inhibition zones ranging from 7.00 ± 0.00 to 10.00 ± 0.00 mm. MIC and MBC assays were conducted to assess the bacteriostatic and bactericidal effects of the leaf extract at a concentration range of 0.08 to 2.50 mg/mL, and > 5.00 mg/mL, respectively. The stability of the leaf extract was also measured at different temperatures and pH conditions by disc diffusion assay with the minimum inhibition zone of 7.00 ± 0.00 mm. The application of P. guajava leaf extract (0.05, 0.50, and 5.00%) on beef samples resulted in a continuous decrease in Total Plate Count during 14-day storage at refrigerated (4.0 ± 2.0°C) and freezing (-18.0 ± 2.0°C) temperatures. The results revealed that P. guajava leaf extract can effectively serve as a natural meat preservative to prolong the shelf life of the treated beef up to 14 days.
Determinants of under-five anaemia in the high prevalence regions of Ghana

Introduction: Anaemia is a serious public health issue that mostly affects children and women throughout their lives, resulting in a high morbidity and mortality burden. It is the third most dominant cause of hospital admission among children under-five in Ghana and the fourth leading cause of under-five mortality in Ghana. This study aims to identify the determinants of under-five anaemia in the high prevalent regions of Ghana using the Ghana Malaria Indicator Survey (2019 GMIS). Methods: An analytic cross-sectional study was conducted using data from the Ghana Malaria Indicator Survey (2019 GMIS). The data was analysed using SPSS version 20. The relationship between the dependent and independent variables was established using the chi-square test and binary logistic regression model. A p-value of 0.05 was used to determine the statistical significance of the study. Results: There were 913 eligible under-five children for this study, with 50.2% males and 49.8% females. The prevalence of under-five anaemia recorded in this current study for the three northern regions was 68.0%. The region with dominant (72.9%) prevalence was the Upper East region. Children of lower age group were more likely to be diagnosed with anaemia (P < 0.05). Children with female household heads were 35% less likely to be diagnosed with anaemia (AOR=0.65, 95% C.I.= 0.421-0.995). Those who had mothers with higher educational attainment were 79% less likely to be diagnosed with anaemia (AOR=0.21, 95% C.I.=0.085–0.541). Finally, those with history of fever in the last weeks were 62% more likely (AOR=1.62, 95% C.I. = 1.155–2.282). Conclusions: The high prevalence in the three northern regions of Ghana can be corrected with women empowerment through higher formal educational achievement and improved income status.
The Qigong of Prolong Life With Nine Turn Method Relieve Fatigue, Sleep, Anxiety and Depression in Patients With Chronic Fatigue Syndrome: A Randomized Controlled Clinical Study

Background Chronic fatigue syndrome (CFS) is a complex disease of unknown etiology and mechanism. The purpose of this study was to investigate the effect of Prolong Life with Nine Turn Method (PLWNT) Qigong exercise on CFS focusing on fatigue, sleep quality, depression, and anxiety. Methods A total of 90 participants diagnosed with CFS were randomly assigned into two parallel groups: PLWNT and cognitive behavioral therapy (CBT). The participants in the PLWNT or CBT group participated in qigong exercise or cognitive behavior education program, respectively, once a week in-person and were supervised online during the remaining 6 days at home, over 12 consecutive weeks. The primary outcome was fatigue (Multi-dimensional Fatigue Inventory 20 [MFI-20]), and secondary outcomes were sleep quality (Pittsburgh Sleep Quality Index [PSQI]), anxiety, depression (Hospital Anxiety and Depression Scale [HADS]), and changes in the Neuropeptide Y (NPY) of peripheral blood. Results The within-group comparisons of the PLWNT and CBT groups revealed significant improvement in both groups in MFI-20, PSQI, and HADS scores (P < 0.05). No significant difference were found between the PLWNT and CBT groups, even though the effective rate of the PLWNT group was 62.22%, which is slightly than 50.00% of the CBT group. The fatigue scores in the PLWNT group were positively correlated with sleep degree (r = 0.315) and anxiety degree (r = 0.333), only anxiety degree (r = 0.332) was found to be positively correlated with fatigue in the CBT group. The analysis of peripheral blood showed that NPY decreased after PLWNT intervention but increased significantly in the CBT. Conclusion The PLWNT qigong exercise has potential to be an effective rehabilitation method for CFS symptoms including fatigue, sleep disturbance, anxiety, and depression. Future studies should expand study sample size for in-depth investigation to determine the optimal frequency and intensity of PLWNT qigong intervention in CFS patients. The study was registered in the ClinicalTrials.gov database on April 12, 2018, with registration number NCT03496961.
A wave automaton for time-dependent wave propagation in random media (Erratum)

A new model is constructed for studying time-dependent properties of wave propagation in random media. Similarly to a cellular automaton, local rules control the dynamical propagation of the waves which exactly obeys a unitarity condition, thus conserving the norm of the wave function and the energy at all times. By reaching times of order 106 times the inverse bandwidth in large systems (512 × 512), the model is used to study the transient dynamics in two dimensions of an impulse which becomes localized at large times.
Clinico-epidemiological profile and outcomes of adults with COVID-19: A hospital-based retrospective study in Kerala, India

Introduction: The clinical and epidemiological presentations of patients with coronavirus disease 2019 (COVID-19) in India is still not well explored. We studied the epidemiological and clinical profile and outcomes of COVID-19 patients admitted to a tertiary care private hospital in Kerala, India. Methods: In this retrospective study, we analyzed data of 476 adult (≥18 years) COVID-19 patients admitted to a tertiary care hospital in Kerala from September 1, 2020 to March 31, 2021. The patients were categorized into mild, moderate, and severe cases and followed till discharge or death. Data were analyzed using Statistical Package for the Social Sciences (SPSS) version 23.0 with a significance set at P < 0.05. Results: The median age was 57 years (56% men). Mild, moderate, and severe cases accounted for 17%, 65%, and 18%, respectively. Around 75% had at least one comorbidity, and 51% had multiple comorbidities. The most common comorbidities were diabetes (45%), hypertension (44%), dyslipidemia (15%), and cardiac problems (12%). The elevated D-dimer values among patients in different categories were significantly different, with 74% in severe, 46% in moderate, and 19% in mild category patients. Serum ferritin, C-reactive protein, lactic acid dehydrogenase, and neutrophil to lymphocyte ratio values were significantly higher for severely ill patients. Thirty deaths (67% men) occurred during the study period, with a case fatality rate of 6.3%. Mortality mainly happened in the older age group (80%) and those with multimorbidity (90%). Conclusion: Age and multimorbidity are the major contributing factors for death in hospitalized COVID-19 patients. Generalization of the findings necessitates well-designed large-scale studies.
Reproductive Senescence in Two Lemur Lineages

The relationship between age and reproductive performance is highly variable across species. Humans and some cetaceans exhibit an extreme form of reproductive senescence in that female reproduction ceases years or even decades before average life expectancy is reached. However, neither the existence of reproductive senescence in some taxa nor its absence in others is fully understood. Comparative data from other long-lived mammals may contribute to a more comprehensive understanding of the evolution of menopause, but data from wild primates, in particular, are scarce. We therefore investigated age-related female reproductive performance in two wild sympatric populations of Malagasy primates: Verreaux’s sifakas (Propithecus verreauxi) and redfronted lemurs (Eulemur rufifrons), which have a maximal longevity of more than 20 years. Based on 25 years of long-term demographic data, we extracted information on reproductive output of 38 female Verreaux’s sifakas and 42 female redfronted lemurs. We modeled variation in female reproductive performance and interbirth intervals as a function of age, the number of adult females within a group to account for female competition, and rainfall as a proxy for annual variation in food availability. We also compared our results for these two species with data on captive populations of the same two genera that are buffered from fluctuations in environmental variables. Our analyses disclosed statistical evidence for reproductive senescence in three out of four populations (captive Coquerel’s sifakas, wild redfronted lemurs, and captive red lemurs) but not for wild Verreaux’s sifakas. Compared to wild populations, reproductive senescence was therefore not less pronounced in captive animals, even though the latter are buffered from environmental adversities. In wild redfronted lemurs, mothers were more likely to give birth in years with more rainfall, but neither the number of co-resident females, nor annual rainfall did predict variation in the probability of giving birth in wild Verreaux’s sifakas. Thus, our study contributes valuable comparative information on reproductive senescence in a basal group of primates, and offers insights into the modulating effects of environmental, social and phylogenetic factors on patterns and dynamics of age-specific female reproduction.
Highly efficient CRISPR systems for loss-of-function and gain-of-function research in pear calli

Abstract CRISPR/Cas systems have been widely used for genome engineering in many plant species. However, their potentials have remained largely untapped in fruit crops, particularly in pear, due to the high levels of genomic heterozygosity and difficulties in tissue culture and stable transformation. To date, only a few reports on the application of the CRISPR/Cas9 system in pear have been documented, and have shown very low editing efficiency. Here we report a highly efficient CRISPR toolbox for loss-of-function and gain-of-function research in pear. We compared four different CRISPR/Cas9 expression systems for loss-of-function analysis and identified a potent system that showed nearly 100% editing efficiency for multi-site mutagenesis. To expand the targeting scope, we further tested different CRISPR/Cas12a and Cas12b systems in pear for the first time, albeit with low editing efficiency. In addition, we established a CRISPR activation (CRISPRa) system for multiplexed gene activation in pear calli for gain-of-function analysis. Furthermore, we successfully engineered the anthocyanin and lignin biosynthesis pathways using both CRISPR/Cas9 and CRISPRa systems in pear calli. Taking these results together, we have built a highly efficient CRISPR toolbox for genome editing and gene regulation, paving the way for functional genomics studies as well as molecular breeding in pear.
Kinect-Based Assessment of Lower Limbs during Gait in Post-Stroke Hemiplegic Patients: A Narrative Review

The aim of this review was to present an overview of the state of the art in the use of the Microsoft Kinect camera to assess gait in post-stroke individuals through an analysis of the available literature. In recent years, several studies have explored the potentiality, accuracy, and effectiveness of this 3D optical sensor as an easy-to-use and non-invasive clinical measurement tool for the assessment of gait parameters in several pathologies. Focusing on stroke individuals, some of the available studies aimed to directly assess and characterize their gait patterns. In contrast, other studies focused on the validation of Kinect-based measurements with respect to a gold-standard reference (i.e., optoelectronic systems). However, the nonhomogeneous characteristics of the participants, of the measures, of the methodologies, and of the purposes of the studies make it difficult to adequately compare the results. This leads to uncertainties about the strengths and weaknesses of this technology in this pathological state. The final purpose of this narrative review was to describe and summarize the main features of the available works on gait in the post-stroke population, highlighting similarities and differences in the methodological approach and primary findings, thus facilitating comparisons of the studies as much as possible.