Miracle-dz/UniFAR-FTUs huggingface dataset:
Prevalence of occupational exposure to needle‐stick injury and associated factors among healthcare workers of developing countries: Systematic review

Abstract Background Healthcare workers are at high risk of infection from blood‐borne pathogens, such as Hepatitis B and C Virus, and Human Immunodeficiency. Occupational exposure to needle‐stick injuries (NSIs) continue to have a major health problem in the healthcare systems of developing countries. Thus, this review article aimed to provide the evidence on the prevalence of NSI and associated factors among healthcare workers of developing countries. Methods The studies published from 2012 to 2019 were identified through systematic searches of electronic databases such as PubMed, Google Scholar, CINAHL, MEDLINE, Scopus, Med Nar, and Science Direct. The MeSH terms and/or keywords was used in conjunction with “AND” or “OR” (Boolean logic operators). All identified keywords and an index terms were checked across the included databases. Assessment and evaluation were taken to confirm the quality and relevance of the included articles, followed by extraction and analysis of data. Result Overall, 2021 articles were identified using specified search terms from the initial searches of the literature (2012‐2019). A total of 13 articles met eligibility criteria were included in the review. Among 6513 participants, 1009 and 2201 participants involved to determine 1‐year and throughout career prevalence, respectively. The prevalence of NSI ranged from 19.9% to 54.0% with an overall prevalence of 35.7% and 38.5 to 100% with an overall prevalence of 64.1% in the previous 1 year and throughout career, respectively. Sex, workload, needle recapping, overuse of injection, and practice of universal precautions, training, occupation, working experience, and personal protective equipment were among the factors associated with the prevalence of NSIs in developing countries. Conclusion The review indicated that NSIs have been identified as one of the most serious issues that affect the health and well‐being of healthcare workers in the majority of healthcare systems of developing countries. There is a need to apply safety practices or other measures to reduce the risk of NSIs.
Plant Defence Mechanisms Are Modulated by the Circadian System

Simple Summary The circadian clock is an endogenous time keeping mechanism found in living organisms and their respective pathogens. Numerous studies demonstrate that rhythms generated by this internal biological oscillator regulate and modulate most of the physiological, developmental, and biochemical processes of plants. Importantly, plant defence responses have also been shown to be modulated by the host circadian clock and vice versa. In this review we discuss the current understanding of the interactions between plant immunity and the circadian system. We also describe the possibility of pathogens directly or indirectly influencing plants’ circadian rhythms and suggest that these interactions could help us devise better disease management strategies for plants. Our review raises further research questions and we conclude that experimentation should be completed to unravel the complex mechanisms underlying interactions between plant defence and the circadian system. Abstract Plant health is an important aspect of food security, with pathogens, pests, and herbivores all contributing to yield losses in crops. Plants’ defence against pathogens is complex and utilises several metabolic processes, including the circadian system, to coordinate their response. In this review, we examine how plants’ circadian rhythms contribute to defence mechanisms, particularly in response to bacterial pathogen attack. Circadian rhythms contribute to many aspects of the plant–pathogen interaction, although significant gaps in our understanding remain to be explored. We conclude that if these relationships are explored further, better disease management strategies could be revealed.
Haematological and Biochemical Parameters of Captive Siberian Tigers (Panthera tigris altaica) from the Heilongjiang Province, China

Abstract Haematological and biochemical parameters play important roles in safeguarding animal health and preventing disease, but the blood reference values of many wild animals are still unknown. Recently, few descriptions of the blood parameters of Siberian tigers (Panthera tigris altaica) have been reported because these tigers comprise an endangered species; however, it is extremely difficult to obtain blood samples necessary for these analyses. This study presents 14 haematological and 16 biochemical parameters of 133 Siberian tigers, of which 112 and 21 were from Heilongjiang Siberian Tiger Park (HB) and Hailin Siberian Tiger Park (HD), China, respectively. Our study is the first to determine the following parameters in Siberian tigers: red blood cell volume distribution width, platelet count, mean platelet volume, amylase (AMY), sodium/potassium, globulin and albumin/globulin levels. As the data for total bilirubin and AMY were not statistically significant, no statistical analysis was conducted for these parameters. Few parameters were significantly different according to sex and region (p < 0.05). The concentration of alkaline phosphatase decreased with age, whereas the creatinine (CREA) increased with age. The CREA concentration of tigers raised in HB was much lower than that of tigers raised in HD. The data obtained in this study provide a reference for monitoring the health of wild and captive Siberian tigers and will add important information to the standards for haematological and biochemical parameters of wild felines.
Identification of long non-coding RNA signatures for squamous cell carcinomas and adenocarcinomas

Studies have demonstrated that both squamous cell carcinomas (SCCs) and adenocarcinomas (ACs) possess some common molecular characteristics. Evidence has accumulated to support the theory that long non-coding RNAs (lncRNAs) serve as novel biomarkers and therapeutic targets in complex diseases such as cancer. In this study, we aimed to identify pan lncRNA signatures that are common to squamous cell carcinomas or adenocarcinomas with different tissues of origin. With the aid of elastic-net regularized regression models, a 35-lncRNA pan discriminative signature and an 11-lncRNA pan prognostic signature were identified for squamous cell carcinomas, whereas a 6-lncRNA pan discriminative signature and a 5-lncRNA pan prognostic signature were identified for adenocarcinomas. Among them, many well-known cancer relevant genes such as MALAT1 and PVT1 were included. The identified pan lncRNA lists can help experimental biologists generate research hypotheses and adopt existing treatments for less prevalent cancers. Therefore, these signatures warrant further investigation.
Accurate mapping of mitochondrial DNA deletions and duplications using deep sequencing

Deletions and duplications in mitochondrial DNA (mtDNA) cause mitochondrial disease and accumulate in conditions such as cancer and age-related disorders, but validated high-throughput methodology that can readily detect and discriminate between these two types of events is lacking. Here we establish a computational method, MitoSAlt, for accurate identification, quantification and visualization of mtDNA deletions and duplications from genomic sequencing data. Our method was tested on simulated sequencing reads and human patient samples with single deletions and duplications to verify its accuracy. Application to mouse models of mtDNA maintenance disease demonstrated the ability to detect deletions and duplications even at low levels of heteroplasmy.
Nitrogen Attenuation in Septic System Plumes

The persistence of inorganic nitrogen is assessed in a set of 21 septic system plumes located in Ontario, Canada, that were studied over a 31‐year period from 1988 to 2019. In the plume zones underlying the drainfields, site mean NO3− values averaged 34 ± 27 mg N/L and exceeded the nitrate drinking water limit (DWL) of 10 mg N/L at 16 of 21 sites. In plume zones extending up to 30 m downgradient from the drainfields, site mean NO3− values averaged 24 ± 20 mg N/L and exceeded the DWL at 9 of 13 sites. Site mean total inorganic nitrogen (TIN; NH4+ + NO3− − N) removal averaged 34 ± 26% in the drainfield zones and 36 ± 44% in the downgradient plume zones, indicating that much of the removal occurred within the drainfields. Removal was much higher at nine sites where drainfield TIN included >10% NH4+ (62 ± 25% removal). TIN removal was not correlated with wastewater loading rate, system age, or sediment carbonate mineral content, but was correlated with water table depth, where shallower water table sites had generally less complete wastewater oxidation. At many of these sites, both NO3− and NH4+ were present together in the plumes and were lost concomitantly, suggesting that the anammox reaction was making an important contribution to the observed TIN loss. When groundwater nitrate contamination is a concern, considering on‐site treatment system designs that lead to a lesser degree of wastewater oxidation, could be a useful approach for enhancing N removal.
Modelling the physiological relevance of sucrose export repression by an Flowering Time homolog in the long‐distance phloem of potato

Abstract Yield of harvestable plant organs depends on photosynthetic assimilate production in source leaves, long‐distance sucrose transport and sink‐strength. While photosynthesis optimization has received considerable interest for optimizing plant yield, the potential for improving long‐distance sucrose transport has received far less attention. Interestingly, a recent potato study demonstrates that the tuberigen StSP6A binds to and reduces activity of the StSWEET11 sucrose exporter. While the study suggested that reducing phloem sucrose efflux may enhance tuber yield, the precise mechanism and physiological relevance of this effect remained an open question. Here, we develop the first mechanistic model for sucrose transport, parameterized for potato plants. The model incorporates SWEET‐mediated sucrose export, SUT‐mediated sucrose retrieval from the apoplast and StSP6A‐StSWEET11 interactions. Using this model, we were able to substantiate the physiological relevance of the StSP6A‐StSWEET11 interaction in the long‐distance phloem for potato tuber yield, as well as to show the non‐linear nature of this effect.
The crystal and molecular structure of zinc(II) monoglycerolate

An X-ray crystallographic analysis of zinc(II) monoglycerolate has been carried out. The crystals are monoclinic, space group P21/c with a 8.110(1), b 6.404(1), c 8.714(2)A β 93.44(1)o. The structure was refined by full-matrix least-squares techniques to an R factor (on 519F) of 0.019. The analysis reveals that the zinc atoms bridge the glycerolate molecules forming polymeric sheets lying parallel to the bc plane. Only aliphatic hydrogens are disposed into the space between the layers, a fact consistent with the lubricity and hydrophobic nature of the solid complex and the observed facile lamellar cleavage perpendicular to the a axis.
Antibacterial Cotton Fabric Functionalized with Copper Oxide Nanoparticles

Textiles functionalized with cupric oxide (CuO) nanoparticles have become a promising option to prevent the spread of diseases due to their antimicrobial properties, which strongly depend on the structure and morphology of the nanoparticles and the method used for the functionalization process. This article presents a review of work focused on textiles functionalized with CuO nanoparticles, which were classified into two groups, namely, in situ and ex situ. Moreover, the analyzed bacterial strains, the resistance of the antimicrobial properties of textiles to washing processes, and their cytotoxicity were identified. Finally, the possible antimicrobial mechanisms that could develop in Gram-positive and Gram-negative bacteria were described.
Procoagulant Disorders in Patients with Newly Diagnosed Pancreatic Adenocarcinoma

Background and objectives: Cancer coagulopathy is thought to be partially due to the up-regulation of tissue factor (TF), thrombin-antithrombin complex (TAT) and soluble P-selectin (sP-selectin). The purpose of this study was to evaluate the clinical significance of TF, TAT and sP-selectin in patients with pancreatic cancer. Materials and methods: The study included 93 subjects: 73 newly diagnosed patients with pancreatic adenocarcinoma (42 with stage I-III and 31 with metastatic cancer (stage IV)) and a control group of 20 healthy subjects. Analyzed patients were hospitalized in the Department of Digestive Tract Diseases, Medical University of Lodz or in the Department of Digestive Tract Surgery, Silesian University, Katowice, Poland. All laboratory parameters were measured using ELISA procedures. Results: TF plasma levels were detectable in all patients and were significantly higher in metastatic cancer compared to stage I-III patients and the control group (p < 0.05). In patients with pancreatic adenocarcinoma, the median levels of TAT were also elevated compared to the control group. Moreover, patients with metastases had significantly higher TAT concentration compared to the I-III cancer group. On the other hand, only the metastatic patients group showed significantly higher plasma sP-selectin levels compared to the controls (p = 0.009), whereas there was no difference between localized and metastatic cancer patients. Conclusions: The coagulation disorders are present in the majority of patients with pancreatic adenocarcinoma already at the diagnosis stage and reflect cancer progression and spread.
Association of Hospital-Diagnosed Infections and Antibiotic Use With Risk of Developing Guillain-Barré Syndrome

Objective To determine whether hospital-diagnosed and community-treated infections are important Guillain-Barré syndrome (GBS) risk factors, we investigated the magnitude and duration of associated GBS risk. Methods We conducted a nationwide population-based case–control study of all patients with first-time hospital-diagnosed GBS in Denmark between 1987 and 2016 and 10 matched population controls per case. Hospital-diagnosed infections were determined in the 1987–2016 period and community antibiotic prescriptions in the 2004–2016 period. We used conditional logistic regression to examine the relative risk of GBS associated with having a recent infection. Results Hospital-diagnosed infections within 60 days were observed in 4.3% of 2,414 GBS cases vs 0.3% of 23,909 controls, with a matched odds ratio (OR) of 13.7 (95% confidence interval [CI], 10.2–18.5). The strongest association with subsequent GBS was observed for lower respiratory tract infection, gastrointestinal tract infection, and septicemia. Community antibiotic prescriptions within 60 days were observed in 22.4% of 1,086 GBS cases and 7.8% of 10,747 controls, with a matched OR of 3.5 (95% CI, 3.0–4.1). The risk of GBS declined considerably with time since infection, with high ORs of 21.3 (95% CI, 14.5–31.2) and 4.7 (95% CI, 3.9–5.7) observed within the first month after a hospital-diagnosed infection and a community antibiotic prescription, respectively. However, GBS risk remained increased 2.4-fold (95% CI, 1.1–5.5) and 1.5-fold (95% CI, 1.2–2.0) even in the fifth month after infection. Conclusion There is a strong, temporal association between community antibiotic use and especially infections necessitating hospitalization and risk of subsequent GBS.
Registered report protocol: Quantitative analysis of septin Cdc10-associated proteome in Cryptococcus neoformans

Cryptococcus neoformans is a pathogenic basidiomycetous yeast that primarily infects immunocompromised individuals. C. neoformans can thrive during infections due to its three main virulence-related characteristics: the ability to grow at host temperature (37°C), formation of carbohydrate capsule, and its ability to produce melanin. C. neoformans strains lacking septin proteins Cdc3 or Cdc12 are viable at 25°C; however, they fail to proliferate at 37°C and are avirulent in the murine model of infection. The basis of septin contribution to growth at host temperature remains unknown. Septins are a family of conserved filament-forming GTPases with roles in cytokinesis and morphogenesis. In the model organism Saccharomyces cerevisiae septins are essential. S. cerevisiae septins form a higher order complex at the mother-bud neck to scaffold over 80 proteins, including those involved in cell wall organization, cell polarity, and cell cycle control. In C. neoformans, septins also form a complex at the mother-bud neck but the septin interacting proteome in this species remains largely unknown. Moreover, it remains possible that septins play other roles important for high temperature stress that are independent of their established role in cytokinesis. Therefore, we propose to perform a global analysis of septin Cdc10 binding partners in C. neoformans, including those that are specific to high temperature stress. This analysis will shed light on the underlying mechanism of survival of this pathogenic yeast during infection and can potentially lead to the discovery of novel drug targets.
Prognostic role of left atrial enlargement in patients with implantable cardioverter defibrillators for primary prevention

Abstract Purpose Left atrial volume index (LAVI) is a predictor of heart failure and adverse events, irrespective of left ventricular systolic function. The role of LAVI in the prediction of appropriate implantable cardioverter-defibrillator (ICD) therapies is currently unclear and was the focus of this study. Methods Consecutive heart failure patients with ischaemic (ICM) or idiopathic (DCM) aetiology receiving ICD for primary prevention were included. The primary endpoint was the occurrence of appropriate ICD therapies (ATs): shocks or antitachycardia pacing (ATP). Inappropriate ICD shocks were also assessed as secondary endpoint. Results Among 198 included patients, severe left atrial dilatation (SLAE = LAVI ≥ 60 ml/m2) was present in 54 (27%). SLAE patients had a higher prevalence of NYHA class ≥ III, severe mitral regurgitation and atrial fibrillation history. During a median follow-up of 45 months (IQR 25–68), ATs occurred more frequently in SLAE group (33% vs. 15%, p = .007) as well as appropriate shocks (24% vs. 10%, p = .014). At multivariate analysis SLAE was an independent predictor of ATs (OR 3.19, 95% CI 1.38–7.38, p = .007). Inappropriate shocks were associated with AF during implantation (p = .03), but not with SLAE (p = .009). Conclusion In DCM or ICM patients candidate to receive an ICD for primary prevention, a severely enlarged left atrium is a predictive factor for ATs (shocks or ATP). The risk of inappropriate shocks was increased in patients with atrial fibrillation, rather than SLAE.
Weight Communication: How Do Health Professionals Communicate about Weight with Their Patients in Primary Care Settings?

ABSTRACT This study aimed to investigate how health professionals (HPs) communicate about weight with their patients. Semi-structured interviews were conducted with 33 health professionals (7 family physicians, 13 nurse practitioners, and 13 dietitians) working in multidisciplinary healthcare settings in Canada. Thematic analysis revealed four main approaches used by HPs to communicate about weight: 1) Analyzing patient perspectives, 2) focusing on overall health rather than weight, 3) directly addressing the topic, and 4) avoiding the topic. The approach chosen was influenced by HPs’ outlook on obesity; for example, those who believed obesity to be a chronic disease did not hesitate to communicate about weight. However, some HPs who reported having obesity mentioned avoiding the topic of weight with their patients and emphasized the importance of establishing a trusting relationship with patients before addressing the topic. The approach chosen by HPs also seemed to be influenced by patient receptiveness, level of readiness, and motivation. Weight communication can be sensitive and the approach used to begin the topic of weight may differ based on patient- and HP factors. Future clinical practice guidelines may benefit from shifting toward communicating about modifiable risk factors rather than weight.
Should I Do This? : Factors Influencing the Decision to Raise Grandchildren among Custodial Grandparents.

BACKGROUND AND OBJECTIVES Grandparents often step in to raise grandchildren when the middle generation is unavailable or unwilling. Although the consequences of raising grandchildren are well researched, little is known about the factors influencing grandparents' decisions to raise grandchildren. The objectives for this study were to (1) explore the factors that influence decision-making processes among grandparent caregivers, and (2) investigate the extent to which these factors reflect a multi-faceted, dualistic framework. RESEARCH DESIGN AND METHODS Data were collected from 108 custodial grandparents who answered an open-ended question about their decision to raise grandchildren. RESULTS Content analyses of participants' answers yielded 15 factorial dimensions defined in terms of their negative/push or positive/pull poles, as it related to their decision to raise grandchildren. Chi Square tests were used to examine each dimension to the extent to which elicited frequencies were differentially represented for positive versus negative poles. For 12 dimensions, the negative decisional parameters outweighed those that were positive. DISCUSSION AND IMPLICATIONS Grandparents report both positive and negative parameters when reflecting on their decision to raise grandchildren. These results call for further research to advance the understanding of the decision-making processes that caregiving grandparents make and the role that they may have on their experiences.
Rapid and ultrasensitive detection of circulating human papillomavirus E7 cell-free DNA as a cervical cancer biomarker

Circulating cell-free DNA (cfDNA) has attracted attention as a non-invasive biomarker for diagnosing and monitoring various cancers. Given that human papillomavirus (HPV) DNA integration and overexpression of E6/E7 oncogenes are pivotal events for carcinogenesis, we sought to determine if HPV E7 cfDNA could serve as a specific biomarker for cervical cancer detection. We applied droplet digital PCR (ddPCR) to quantify HPV16/18 E7 cfDNA from the serum of patients with cervical cancer, cervical intraepithelial neoplasia, and controls. HPV16/18 E7 cfDNA was highly specific for cervical cancer, displaying 30.77% sensitivity, 100% specificity, and an area under the curve of 0.65. Furthermore, we developed a sensitive isothermal detection of HPV16/18 E7 and the PIK3CA WT reference gene based on recombinase polymerase amplification combined with a lateral flow strip (RPA-LF). The assay took less than 30 min and the detection limit was 5–10 copies. RPA-LF exhibited 100% sensitivity and 88.24% specificity towards HPV16/18 E7 cfDNA in clinical samples. The agreement between RPA-LF and ddPCR was 83.33% (κ = 0.67) for HPV16 E7 and 100% (κ = 1.0) for HPV18 E7, indicating a good correlation between both tests. Therefore, we conclude that HPV E7 cfDNA represents a potential tumor marker with excellent specificity and moderate sensitivity for minimally invasive cervical cancer monitoring. Moreover, the RPA-LF assay provides an affordable, rapid, and ultrasensitive tool for detecting HPV cfDNA in resource-limited settings.