Miracle-dz/UniFAR-FTUs huggingface dataset:
Early ART-treated perinatally HIV-infected seronegative children demonstrate distinct long-term persistence of HIV-specific T and B cell memorys

Objective: investigate long-term persistence of HIV specific lymphocyte immunity in perinatally HIV infected children treated within the first year of life. Design: 20 perinatally HIV infected children who received ART-therapy within the first year of life (ET) and with stable viral control (>5 years) were grouped according to their serological response to HIV. Methods: Western blot analysis and ELISA defined 14 HIV seropositive (SP) and 6 seronegative patients (SN). Frequencies of gp140-specific T and B cell, and T cell cytokine production were quantified by flow cytometry in both SN and SP. Transcriptional signatures in purified gp140-Corresponding specific B cell subsets, in response to in vitro stimulation with HIV peptides was evaluated by multiplex RT-PCR. Results: Gp140-specific T- and B-cells persist at similar levels in both groups. A higher production of IL-21 in gp140-specific T cells was found in SP vs. SN (p=0.003). Gene expression in switched IgM-IgD-gp140-specific memory B cells after stimulation with HIV peptides in vitro demonstrated a differential expression of genes involved in signal transduction and activation after BCR/TLR triggering and B cell activation. Genes relating to antibody production (PRDM1) and T-B cognate stimulation (CXCR4, IL21R) were differentially induced after in vitro stimulation in SN vs SP suggesting a truncated process of B cell maturation. Conclusions: HIV-specific memory B and T cells persist in ET regardless their serological status. SN and SP are distinguished by gp140-specific T cell function and by distinct transcriptional signatures of gp140-specific B cells after in vitro stimulation, presumably due to a different antigen exposure. Such qualitative insights may inform future immunotherapeutic interventions.
Inhibition of HDAC6 by tubastatin A disrupts mouse oocyte meiosis via regulating histone modifications and mRNA expression

Histone deacetylase 6 (HDAC6) participates in mouse oocyte maturation by deacetylating α‐tubulin. However, how HDAC6 expression is regulated in oocytes remains unknown. In the present study, we discovered that mouse oocytes had a high level of HDAC6 expression and a low level of DNA methylation status in their promoter region. Then, a selective HDAC6 inhibitor, tubastatin A (Tub‐A) was chosen to investigate the role of HDAC6 in oocyte maturation. Our results revealed that inhibition of HDAC6 caused meiotic progression arrest, disturbed spindle/chromosome organization, and kinetochore–microtubule attachments without impairing spindle assembly checkpoint function. Moreover, inhibition of HDAC6 not only increased the acetylation of α‐tubulin but also elevated the acetylation status of H4K16 and decreased the phosphorylation level of H3T3 and H3S10. Conversely, depressed H3T3 phosphorylation by its kinase inhibitor increased the acetylation level of H4K16. Finally, single cell RNA‐seq analysis revealed that the cell cycle‐related genes CCNB1, CDK2, SMAD3, YWHAZ and the methylation‐related genes DNMT1 and DNMT3B were strongly repressed in Tub‐A treated oocytes. Taken together, our results indicate that HDAC6 plays important roles in chromosome condensation and kinetochore function via regulating several key histone modifications and messenger RNA transcription during oocyte meiosis.
Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea europaea

Ethnopharmacological use of plant natural extracts has been known since ancient times. The optimization of plant molecule extraction is fundamental in obtaining relevant extraction yields. The main purpose of this study was to understand the role of different extraction techniques (solid-liquid, ultrasound, Soxhlet, and microwave) and solvents (water, methanol, ethanol, acetone, dichloromethane, and hexane) on the antimicrobial and antioxidant activities of extracts from Olea europaea (olive) and Acacia dealbata (mimosa). Crude plant extracts were evaluated for their antimicrobial activity against Staphylococcus aureus and Escherichia coli by the disk diffusion method. The antioxidant capacity of the extracts was determined by ABTS (2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods. In terms of extraction yield, ultrasound extraction and the solvents methanol, acetone (O. europaea) or water (A. dealbata) were found to be the best options. However, ethanol and acetone proved to be the best solvents to extract compounds with antimicrobial activity and antioxidant capacity, respectively (regardless of the extraction method employed). Soxhlet and microwave were the best techniques to extract compounds with antimicrobial activity, whereas any of the tested techniques showed the ability to extract compounds with antioxidant capacity. In most of the cases, both plant extracts (mimosa and olive) were more efficient against S. aureus than E. coli. In the present study, both mimosa and olive leaf crude extracts proved to have antimicrobial and antioxidant activities, increasing the demand of these natural products as a source of compounds with health benefits.
Bioenergetics and Biomechanics of Handcycling at Submaximal Speeds in Athletes with a Spinal Cord Injury

Background: This study aimed at comparing bioenergetics and biomechanical parameters between athletes with tetraplegia and paraplegia riding race handbikes at submaximal speeds in ecological conditions. Methods: Five athletes with tetraplegia (C6-T1, 43 ± 6 yrs, 63 ± 14 kg) and 12 athletes with paraplegia (T4-S5, 44 ± 7 yrs, 72 ± 12 kg) rode their handbikes at submaximal speeds under metabolic measurements. A deceleration method (coasting down) was applied to calculate the rolling resistance and frontal picture of each participant was taken to calculate air resistance. The net overall Mechanical Efficiency (Eff) was calculated by dividing external mechanical work to the corresponding Metabolic Power. Results: Athletes with tetraplegia reached a lower aerobic speed (4.7 ± 0.6 m s−1 vs. 7.1 ± 0.9 m s−1, P = 0.001) and Mechanical Power (54 ± 15 W vs. 111 ± 25 W, P = 0.001) compared with athletes with paraplegia. The metabolic cost was around 1 J kg−1 m−1 for both groups. The Eff values (17 ± 2% vs. 19 ± 3%, P = 0.262) suggested that the handbike is an efficient assisted locomotion device. Conclusion: Handbikers with tetraplegia showed lower aerobic performances but a similar metabolic cost compared with handbikers with paraplegia at submaximal speeds in ecological conditions.
Pathogenicity of H5 highly pathogenic avian influenza virus in rooks (Corvus frugilegus)

ABSTRACT Rooks (Corvus frugilegus) are considered migratory crows in Japan. Some rooks share a wintering site in the Izumi plain in Kagoshima Prefecture with hooded cranes (Grus monacha) and white-necked cranes (Grus vipio), which are designated as “endangered” in the International Union for Conservation of Nature (IUCN) Red List of Threatened Species. Highly pathogenic avian influenza (HPAI), caused by H5 subtype viruses, has recently been reported in these crane species in Japan, in conjunction with a massive decrease in their population. In the present study, the pathogenicity of HPAI virus was assessed in rooks to evaluate the likelihood that they are a source of infections in other bird species. One of four rooks intranasally inoculated with A/mandarin duck/Miyazaki/22M807-1/2011 (H5N1) died at 10 days post-inoculation (d.p.i.). The other three rooks exhibited seroconversion but no clinical signs. All the rooks had shed virus by the oral route at <103 50% egg infectious dose/ml until 7 d.p.i. Virus was also recovered from multiple tissues of the rook that succumbed to the infection. These results suggest that rooks are susceptible to infection with H5 HPAI viruses, leading to prolonged viral shedding. The rooks shed the virus at low titres however, indicating that they are likely to function as transmission vectors in wintering bird flocks. The rooks exhibited clear antibody responses against the H5 HPAI virus, and thus serological surveillance of them in the field should be helpful for assessing viral pervasion into the habitats of crane species.
Effect of physical exercise on the functional capacity of children and adolescents submitted to transplantation of hematopoietic stem cells—A systematic review with meta-analysis

Previous studies have shown beneficial effects of physical exercise (PE) in adults submitted to hematopoietic stem cell transplantation (HSCT). Conduct a systematic review about the effects of PE on the functional capacity of children and adolescents submitted to HSCT. The studies were searched in the databases MEDLINE (via PubMed), Central Register of Controlled Trials (Cochrane CENTRAL), EMBASE, LILACS, and Evidence Database in Physical Therapy (PEDro) (CRD42018080093). Two independent reviewers performed the article selection, data extraction, and methodological quality assessment. Randomized and nonrandomized clinical trials comparing PE with usual treatment in children and adolescents aged 3–18 years were included. The risk of bias was assessed using the Cochrane Collaboration tool and ROBINS-I tool, and the overall quality of the evidence was determined by the GRADE system. We included three studies with 91 patients. PE improved the functional capacity assessed by the timed up and down stairs test (MD −1.23 [95% CI, 2.27 to −.20, I2 = 0%]), but there was no significant effect in the six-minute walk test (MD 44.63 [95% CI, −20.86 to 110.13, I2 = 83%]). The benefits regarding quality of life and peripheral muscle strength of these individuals were not clearly demonstrated, but positive responses were observed in relation to the analyzed data. None of the studies evaluated the fatigue. The limitations found were the high heterogeneity between studies, as well as the sample size and the low methodological rigor. PE might be favorable to improve the functional capacity of children and adolescents treated with HSCT. However, further studies are needed to clarify the best PE program.
What evidence is there to support the employment of trained and professionally registered library, information and knowledge workers? A systematic scoping review of the evidence

This report seeks to provide evidence that CILIP can use to advocate on behalf of the information professions. The report addresses three aims: 1. To create an initial evidence base which can be used to advocate on behalf of the information professions. 2. To develop clear propositions outlining the value that trained and professional registered staff provide. 3. To identify options for further research which can be commissioned by CILIP It doe this by: 1. Reporting on a systematic scoping review of the literature to identify the evidence base for trained and professionally registered library, information and knowledge (LIK) workers 2. Mapping the evidence according to library sector 3. Identifying which evidence bases need updating 4. Drawing up a set of propositions from the results of the review and mapping 5. Identifying further research needed and what methods could be used to achieve this.
Applying whole-genome sequencing in relation to phenotype and outcomes in siblings with cystic fibrosis

Variations in disease onset and/or severity have often been observed in siblings with cystic fibrosis (CF), despite the same CFTR genotype and environment. We postulated that genomic variation (modifier and/or pharmacogenomic variants) might explain these clinical discordances. From a cohort of patients included in the Wisconsin randomized clinical trial (RCT) of newborn screening (NBS) for CF, we identified two brothers who showed discordant lung disease courses as children, with one milder and the other more severe than average, and a third, eldest brother, who also has severe lung disease. Leukocytes were harvested as the source of DNA, and whole-genome sequencing (WGS) was performed. Variants were identified and analyzed using in-house-developed informatics tools. Lung disease onset and severity were quantitatively different between brothers during childhood. The youngest, less severely affected brother is homozygous for HFE p.H63D. He also has a very rare PLG p.D238N variant that may influence host–pathogen interaction during chronic lung infection. Other variants of interest were found differentially between the siblings. Pharmacogenomics findings were consistent with the middle, most severely affected brother having poor outcomes to common CF treatments. We conclude that genomic variation between siblings with CF is expected. Variable lung disease severity may be associated with differences acting as genetic modifiers and/or pharmacogenomic factors, but large cohort studies are needed to assess this hypothesis.
Optimality of sparse olfactory representations is not affected by network plasticity

The neural representation of a stimulus is repeatedly transformed as it moves from the sensory periphery to deeper layers of the nervous system. Sparsening transformations are thought to increase the separation between similar representations, encode stimuli with great specificity, maximize storage capacity of associative memories, and provide an energy efficient instantiation of information in neural circuits. In the insect olfactory system, odors are initially represented in the periphery as a combinatorial code with relatively simple temporal dynamics. Subsequently, in the antennal lobe this representation is transformed into a dense and complex spatiotemporal activity pattern. Next, in the mushroom body Kenyon cells (KCs), the representation is dramatically sparsened. Finally, in mushroom body output neurons (MBONs), the representation takes on a new dense spatiotemporal format. Here, we develop a computational model to simulate this chain of olfactory processing from the receptor neurons to MBONs. We demonstrate that representations of similar odorants are maximally separated, measured by the distance between the corresponding MBON activity vectors, when KC responses are sparse. Sparseness is maintained across variations in odor concentration by adjusting the feedback inhibition that KCs receive from an inhibitory neuron, the Giant GABAergic neuron. Different odor concentrations require different strength and timing of feedback inhibition for optimal processing. Importantly, as observed in vivo, the KC–MBON synapse is highly plastic, and, therefore, changes in synaptic strength after learning can change the balance of excitation and inhibition, potentially leading to changes in the distance between MBON activity vectors of two odorants for the same level of KC population sparseness. Thus, what is an optimal degree of sparseness before odor learning, could be rendered sub–optimal post learning. Here, we show, however, that synaptic weight changes caused by spike timing dependent plasticity increase the distance between the odor representations from the perspective of MBONs. A level of sparseness that was optimal before learning remains optimal post-learning.
The Guangzhou Twin Eye Study: 2019 Update

Abstract The primary aim of the Guangzhou Twin Eye Study (GTES) is to explore the impact that genes and environmental inﬂuences have on common eye diseases. Since 2006, approximately 1300 pairs of twins, aged 7–15 years, were enrolled at baseline. Progressive phenotypes, such as cycloplegic refraction, axial length, height and weight, have been collected annually. Nonprogressive phenotypes such as parental refraction, corneal thickness, fundus photo, intraocular pressure and DNA were collected once at baseline. We are collaborating with fellow international twin researchers and psychologists to further explore links with general medical conditions. In this article, we review the history, major findings and future research directions for the GTES.
Transgenic Mice Overexpressing Human STIM2 and ORAI1 in Neurons Exhibit Changes in Behavior and Calcium Homeostasis but Show No Signs of Neurodegeneration

The maintenance of proper cytosolic Ca2+ level is crucial for neuronal survival, and dysregulation of Ca2+ homeostasis is found in a variety of neurological disorders, including Alzheimer’s disease. According to the “Ca2+ hypothesis of aging”, Ca2+ disturbances precede the onset of AD symptoms and lead to neurodegeneration. STIM and ORAI proteins are involved in neuronal physiological and pathological processes as essential components of the store-operated Ca2+ entry. Our previous data suggested that overexpression of STIM2 and ORAI1 might increase basal neuronal cytosolic Ca2+ level. We generated double transgenic mice overexpressing these two genes in neurons, expecting that the increased basal Ca2+ concentration will lead to premature neurodegeneration. We observed changes in Ca2+ homeostasis and electrophysiological properties in acute brain slices of STIM2/ORAI1 neurons. However, we did not observe any augmentation of neurodegenerative processes, as tested by Fluoro-Jade® C staining and assessment of amyloidogenesis. The battery of behavioral tests did not show any signs of accelerated aging. We conclude that changes of calcium homeostasis induced by overexpression of STIM2 and ORAI1 had no substantial adverse effects on neurons and did not lead to early neurodegeneration.
Few-layer metal monochalcogenide saturable absorbers for high-energy Q-switched pulse generation

Two-dimensional layered materials have been widely utilized as nonlinear absorption materials to transfer continue-wave into pulse trains in fiber laser systems. Here, we prepare robust GaSe/GeSe composites with high power bearing capacity as saturable absorbers (SAs) and then investigate their nonlinear optical properties via broadband Z-scan measurement at 800 nm and 1550 nm, respectively. The modulation depths of GaSe/GeSe based SAs are measured to be 11.97% and 7.69% at 1550 nm. After incorporating the GaSe/GeSe SAs into an Erbium-doped fiber laser cavity, passively Q-switched pulse trains could be obtained with repetition rates changing from 83.58 to 136.78 kHz (70.41 to 161.65 kHz). The maximum output power and pulse energy are 52.1 mW/370.67 nJ (GaSe) and 21.6 mW/133.74 nJ (GeSe) under the maximum pump power of 600 mW. The results indicate that GaSe and GeSe possess outstanding thermal stability and could be employed as remarkable saturable absorption materials for high-energy pulses generation.
Ocular Manifestations of Sickle Cell Disease: Signs, Symptoms and Complications

ABSTRACT Background Sickle cell disease is an inherited hematological disorder that can affect any organ in the body including the eyes (1–6). Previous studies on ocular manifestations of sickle cell disease generally included samples of less than 100 patients. In this study, we aim to assess the frequency of different ocular signs, symptoms and complications among sickle cell disease patients. Methods This study was conducted using data from the Cooperative Study of Sickle Cell Disease (CSSCD). Patients with major sickle cell hemoglobinopathies (SS, SC, S β-thal) were eligible for enrollment. Patients from all age groups were included. Patients underwent detailed ophthalmological examination under standardized conditions. Results A total of 1904 patients were included in this study, with a mean age of 27.67 (±11.72) years. 1,802 (96.4%) patients had BCVA of more than 20/40 in the better-seeing eye. On slit lamp examination, the presence of vascular loops and segment, representing a positive conjunctival sign, was the most common reported abnormal finding (54.1%). The most common complication was peripheral retinal artery occlusion detected in225 patients (20.3%) bilaterally and 77 patients (6.9%) unilaterally. Conclusion In this study that included one of the largest samples ever studied to assess ocular complications of sickle cell disease, we identified the frequency and percentages of different ocular signs, symptoms and complications in different age groups.
Depth Image–Based Deep Learning of Grasp Planning for Textureless Planar-Faced Objects in Vision-Guided Robotic Bin-Picking

Bin-picking of small parcels and other textureless planar-faced objects is a common task at warehouses. A general color image–based vision-guided robot picking system requires feature extraction and goal image preparation of various objects. However, feature extraction for goal image matching is difficult for textureless objects. Further, prior preparation of huge numbers of goal images is impractical at a warehouse. In this paper, we propose a novel depth image–based vision-guided robot bin-picking system for textureless planar-faced objects. Our method uses a deep convolutional neural network (DCNN) model that is trained on 15,000 annotated depth images synthetically generated in a physics simulator to directly predict grasp points without object segmentation. Unlike previous studies that predicted grasp points for a robot suction hand with only one vacuum cup, our DCNN also predicts optimal grasp patterns for a hand with two vacuum cups (left cup on, right cup on, or both cups on). Further, we propose a surface feature descriptor to extract surface features (center position and normal) and refine the predicted grasp point position, removing the need for texture features for vision-guided robot control and sim-to-real modification for DCNN model training. Experimental results demonstrate the efficiency of our system, namely that a robot with 7 degrees of freedom can pick randomly posed textureless boxes in a cluttered environment with a 97.5% success rate at speeds exceeding 1000 pieces per hour.
Correlation of urine formaldehyde levels and mini mental state examination scores in Alzheimer's disease

Objective To investigate the correlation of endogenous formaldehyde in urine of elderly patients of Alzheimer's disease(A D) and mini mental state examination(MMSE) scores. Methods Urine formaldehyde level was analyzed by high-pe rformance liquid chromatography(with fluorescence detection) from patients with mild,moderate,and severe AD(aged 65 and over),and age-matched healthy indi viduals(n=30 for each group). Results Urine formaldehyde level was inversely correlated with MMSE scores(rs=-0.676,P0.001),with a marked elevation in pa tients with severe dementia and slight increase in moderate cases. Conclusions Urine formaldehyde level is probably related to cognitive impairments,and might be a potential diagnosis index for AD.