Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression and regulation of TREM-1 in periodontal diseases

Periodontitis is an inflammatory infectious disease that destroys the tooth-supporting tissues. It is caused by multi-species subgingival biofilms that colonize the tooth surface. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia (i.e. ”red complex” bacteria) are characteristic subgingival biofilm species. The Triggering Receptor Expressed on Myeloid cells 1 (TREM-1) is a cell surface receptor of the immunoglobulin super-family, with a role in the amplification of pro-inflammatory cytokine production during infection. This study aimed to investigate TREM-1 mRNA expression in gingival tissues from patients with chronic periodontitis, generalised aggressive periodontitis and healthy subjects, and its correlation with the levels of periodontal pathogens in the tissue. A further aim was to investigate the regulation of TREM-1 in human monocytic cells (MM6) challenged with an in vitro subgingival biofilm model. Gingival tissue TREM-1 expression was increased in both chronic and aggressive periodontitis, compared to health, and correlated with the levels of the ”red complex” species in the tissue. No significant differences were detected between the two forms of periodontitis. Biofilm-challenged MM6 cells exhibited higher TREM-1 expression and secretion compared to controls, irrespective of the presence of the ”red complex”. Engagement or inhibition of TREM-1 affected the capacity of the biofilms to stimulate Interleukin (IL)-1, but not IL-8, secretion by the cells. In conclusion, this study reveals that TREM-1 tissue expression is enhanced in periodontal disease, and correlates with the level of periodontal pathogens. It also provides a mechanistic insight into the regulation of TREM-1 expression and the associated IL-1 production, in biofilm-challenged monocytes. DOI: https://doi.org/10.1111/cei.12397 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-96623 Journal Article Accepted Version Originally published at: Willi, Martin; Belibasakis, Georgios N; Bostanci, Nagihan (2014). Expression and regulation of TREM-1 in periodontal diseases. Clinical and Experimental Immunology, 178(1):190-200. DOI: https://doi.org/10.1111/cei.12397
Extralymphoid CD8 (cid:1) T Cells Resident in Tissue from Simian Immunodeﬁciency Virus SIVmac239 (cid:2) nef-Vaccinated Macaques Suppress SIVmac239 Replication Ex Vivo (cid:1)

Live-attenuated vaccination with simian immunodeﬁciency virus (SIV) SIVmac239 (cid:1) nef is the most successful vaccine product tested to date in macaques. However, the mechanisms that explain the efﬁcacy of this vaccine remain largely unknown. We utilized an ex vivo viral suppression assay to assess the quality of the immune response in SIVmac239 (cid:1) nef-immunized animals. Using major histocompatibility complex-matched Mauritian cynomolgus macaques, we did not detect SIV-speciﬁc functional immune responses in the blood by gamma interferon (IFN- (cid:2) ) enzyme-linked immunospot assay at select time points; however, we found that lung CD8
Prompt neutrino ﬂuxes in the atmosphere with PROSA parton distribution functions

: Eﬀects on atmospheric prompt neutrino ﬂuxes of present uncertainties aﬀecting the nucleon composition are studied by using the PROSA ﬁt to parton distribution functions (PDFs). The PROSA ﬁt extends the precision of the PDFs to low x , which is the kinematic region of relevance for high-energy neutrino production, by taking into account LHCb data on charm and bottom hadroproduction. In the range of neutrino energies explored by present Very Large Volume Neutrino Telescopes, it is found that PDF uncertainties are far smaller with respect to those due to renormalization and factorization scale variation and to assumptions on the cosmic ray composition, which at present dominate and limit our knowledge of prompt neutrino ﬂuxes. A discussion is presented on how these uncertainties aﬀect the expected number of atmospheric prompt neutrino events in the analysis of high-energy events characterized by interaction vertices fully contained within the instrumented volume of the detector, performed by the IceCube collaboration.
Following Anterior Cervical Discectomy and Fusion Prospective Assessment of Recurrent Laryngeal Nerve Injury Correlation of Voice Hoarseness and Vocal Cord Palsy: A

Background: Injury to the recurrent laryngeal nerve (RLN) has been implicated as a common complication following anterior cervical discectomy and fusion (ACDF) surgery. The purpose of this study is to determine the true incidence of voice hoarseness and RLN palsy following ACDF surgery, to determine the reliability of symptoms in the diagnosis of RLN injury, and to evaluate factors related to the development of these symptoms. Methods: All patients undergoing elective (primary or secondary) ACDF surgery at a single institution consented to and enrolled in the present study. All approaches were through the left side. Enrolled patients received both preoperative and postoperative (within 1 month following surgery) laryngoscopy by a fellowship-trained ENT physician for evaluation of RLN function. Patients also responded as to whether they were experiencing postoperative symptoms of dysphagia, aspiration, and voice changes. Results: In total, 108 patients were included in this study. Mean age of the population was 59.2 6 10.7 years and mean body mass index was 31.2 6 7.1 kg/m 2 . Three patients had previously undergone a thyroidectomy, whereas 20 patients had undergone a previous ACDF. Average intubation time for ACDF surgery was 121.6 6 38.5 minutes. After surgery and excluding patients who were experiencing preoperative symptoms, 19 patients (20.4%) complained of dysphagia, 2 patients (1.9%) complained of aspiration symptoms, and 5 patients (4.6%) complained of voice hoarseness. There was no incidence of vocal cord palsy from postoperative laryngoscopy. From multivariate analysis, endotracheal cuff pressure after retractor placement was correlated to postoperative voice hoarseness, dysphagia, and aspiration symptoms. Conclusions: From the results of this prospective study, the RLN remained functional even a month after surgery despite several cases of postoperative dysphagia, aspiration, and voice changes. Endotracheal cuff pressure, number of vertebral levels, body mass index, and intubation time were important variables related to postoperative symptoms. Clinical Relevance: Voice hoarseness does not necessarily indicate recurrent laryngeal nerve injury after ACDF but may be caused by compressive forces on laryngeal tissue during retraction or intubation. Laryngoscopy should be performed in cases with high clinical suspicion.
The effects of anthropomorphism and multimodal biometric authentication on the user experience of voice intelligence

Voice intelligence is a revolutionary “zero-touch” type of human-machine interaction based on spoken language. There has been a recent increase in the number and variations of voice assistants and applications that help users to acquire information. The increased popularity of voice intelligence, however, has not been reflected in the customer value chain. Current research on the socio-technological aspects of human-technology interaction has emphasized the importance of anthropomorphism and user identification in the adoption of the technology. Prior research has also pointed out that user perception toward the technology is key to its adoption. Therefore, this research examines how anthropomorphism and multimodal biometric authentication influence the adoption of voice intelligence through user perception in the customer value chain. In this study we conducted a between-subjects online experiment. We designed a 2 × 2 factorial experiment by manipulating anthropomorphism and multimodal biometric authentication into four conditions, namely with and without a combination of these two factors. Subjects were recruited from Amazon MTurk platform and randomly assigned to one of the four conditions. The results drawn from the empirical study showed a significant direct positive effect of anthropomorphism and multimodal biometric authentication on user adoption of voice intelligence in the customer value chain. Moreover, the effect of anthropomorphism is partially mediated by users' perceived ease of use, perceived usefulness, and perceived security risk. This research contributes to the existing literature on human-computer interaction and voice intelligence by empirically testing the simultaneous impact of anthropomorphism and biometric authentication on users' experience of the technology. The study also provides practitioners who wish to adopt voice intelligence in the commercial environment with insights into the user interface design.
Deconvolution of B cell receptor repertoire in multiple sclerosis patients revealed a delay in tBreg maturation

Background B lymphocytes play a pivotal regulatory role in the development of the immune response. It was previously shown that deficiency in B regulatory cells (Bregs) or a decrease in their anti-inflammatory activity can lead to immunological dysfunctions. However, the exact mechanisms of Bregs development and functioning are only partially resolved. For instance, only a little is known about the structure of their B cell receptor (BCR) repertoires in autoimmune disorders, including multiple sclerosis (MS), a severe neuroinflammatory disease with a yet unknown etiology. Here, we elucidate specific properties of B regulatory cells in MS. Methods We performed a prospective study of the transitional Breg (tBreg) subpopulations with the CD19+CD24highCD38high phenotype from MS patients and healthy donors by (i) measuring their content during two diverging courses of relapsing-remitting MS: benign multiple sclerosis (BMS) and highly active multiple sclerosis (HAMS); (ii) analyzing BCR repertoires of circulating B cells by high-throughput sequencing; and (iii) measuring the percentage of CD27+ cells in tBregs. Results The tBregs from HAMS patients carry the heavy chain with a lower amount of hypermutations than tBregs from healthy donors. The percentage of transitional CD24highCD38high B cells is elevated, whereas the frequency of differentiated CD27+ cells in this transitional B cell subset was decreased in the MS patients as compared with healthy donors. Conclusions Impaired maturation of regulatory B cells is associated with MS progression.
An exploratory assessment of the management of pediatric traumatic brain injury in three centers in Africa

Purpose Traumatic brain injury (TBI) is a leading cause of morbidity and mortality in low- and middle-income countries (LMICs). Hospital care practices of pediatric TBI patients in LMICs are unknown. Our objective was to report on hospital management and outcomes of children with TBI in three centers in LMICs. Methods We completed a secondary analysis of a prospective observational study in children (<18 years) over a 4-week period. Outcome was determined by Pediatric Cerebral Performance Category (PCPC) score; an unfavorable score was defined as PCPC > 2 or an increase of two points from baseline. Data were compared using Chi-square and Wilcoxon rank sum tests. Results Fifty-six children presented with TBI (age 0–17 y), most commonly due to falls (43%, n = 24). Emergency department Glasgow Coma Scale scores were ≤ 8 in 21% (n = 12). Head computed tomography was performed in 79% (n = 44) of patients. Forty (71%) children were admitted to the hospital, 25 (63%) of whom were treated for suspected intracranial hypertension. Intracranial pressure monitoring was unavailable. Five (9%, n = 5) children died and 10 (28%, n = 36) inpatient survivors had a newly diagnosed unfavorable outcome on discharge. Conclusion Inpatient management and monitoring capability of pediatric TBI patients in 3 LMIC-based tertiary hospitals was varied. Results support the need for prospective studies to inform development of evidence-based TBI management guidelines tailored to the unique needs and resources in LMICs.
Effects of replacing Brachiaria hay with either Desmodium intortum or dairy concentrate on animal performance and enteric methane emissions of low-yielding dairy cows

In Africa, cattle are often fed low quality tropical roughages resulting in low-yielding animals with high methane (CH4) emission intensity (EI, g CH4/per unit of product). Supplementation with protein is known to improve the nutritive value of the otherwise low-quality diets. However, animal nutrition studies in East Africa that are accompanied by CH4 emission measurements are lacking. Thus, an animal experiment was conducted to quantify the effect of supplementing cattle fed mainly on low-quality Urochloa brizantha hay (control diet; CON; crude protein (CP) = 7.4%) or supplemented with either a tannin-rich leguminous fodder, Desmodium intortum hay (DES) or a commercial dairy concentrate (CUBES) on voluntary dry matter intake (DMI), nutrient apparent total tract digestibility, nitrogen (N) retention, enteric CH4 production and animal performance (milk and average daily gain). Twelve mid-lactating crossbred (Friesian × Boran) cows (initial liveweight = 335 kg) were used in a 3×3 (Period × Diet) Latin square design with each period running for four weeks. Compared to CON, DES decreased nutrient (DM, OM, CP) intake, apparent total tract digestibility and daily milk yield. In contrast, CUBES increased nutrient intake and animal performance compared to CON, while nutrients’ apparent total tract digestibility was not different, except for CP digestibility that increased. Compared to CON, DES and CUBES improved overall N retention by the animals as a proportion of N intake. The DES diet compared with CON and CUBES, shifted the proportion of N excretion via urine to the fecal route, likely because of its tannin content. Both DES and CUBES, compared to CON, reduced methane yield (MY, g CH4/kg DMI) by 15% and 9%, respectively. The DES diet reduced absolute enteric CH4 emissions by 26% while CUBES increased emissions by 11% compared to CON. Based on the present findings, high supplementation levels (>50%) of Desmodium intortum hay is not recommended especially when the basal diet is low in CP content. Supplementation with lower levels of better managed Desmodium intortum forage however, need to be investigated to establish optimal inclusion levels that will improve animal productivity and reduce environmental impact of livestock in smallholder tropical contexts.
Apatinib for recurrent/progressive glioblastoma multiforme: A salvage option

Purpose: The recurrent/progressive glioblastoma multiforme (GBM) carries a dismal prognosis and the definitive treatment strategy has not yet been established. This study aimed to assess the efficacy and safety of apatinib in recurrent/progressive GBM patients. Materials and methods: The clinical data of 19 recurrent/progressive GBM patients who received apatinib treatment from November 2015 to December 2019 at Sun Yat-sen University Cancer Center were collected retrospectively in this study. Objective response rate (ORR), disease control rate (DCR), progression-free survival (PFS), overall survival (OS), and treatment-related adverse events (AEs) were reviewed and assessed. Results: The overall ORR was 52.6%, and the DCR was 73.7%. Median PFS and OS were 5.1 and 10.4 months, respectively. The 6-month PFS and OS rates were 38.9% and 68.4%, respectively. The 12-month PFS and OS rates were 16.7% and 36.8%, respectively. The treatment-related toxicities were generally well-tolerated. The most common grade 3/4 AEs were hand-foot syndrome (36.8%) and hypertension (21.1%). Conclusion: Our study showed that apatinib therapy provided a better salvaging option for recurrent/progressive GBM patients and the toxicity was manageable.
Erectile dysfunction: Is platelet-rich plasma the new frontier for treatment in patients with erectile dysfunction? A review of the existing evidence

Erectile dysfunction (ED) is one of the commonest disorders in adult males and affects 12–19% of men of reproductive age. Only few studies have evaluated the impact of ED on men and couples with infertility—these studies report higher rates of ED in this sub-group of men compared to the general population, with the prevalence of ED in men diagnosed with male infertility ranging from 6.7 to 61.6%. Nevertheless, ED is considered a rare cause of male infertility, accounting for about 0.4–5% of all causes of male infertility. ED remains a poorly treated condition globally and current therapies, like oral medication, offer only temporary symptomatic relief and do not influence disease progression—patients are potentially on lifelong treatment, with ED worsening over time. In contrast, regenerative medicine may potentially reverse or halt the progression of ED processes. In this article, we review the evidence for intracavernosal injections of platelet-rich plasma (PRP) in the treatment of ED.
Evidence for dynamic attentional bias toward positive emotion-laden words: A behavioral and electrophysiological study

There has been no consensus on the neural dissociation between emotion-label and emotion-laden words, which remains one of the major concerns in affective neurolinguistics. The current study adopted dot-probe tasks to investigate the valence effect on attentional bias toward Chinese emotion-label and emotion-laden words. Behavioral data showed that emotional word type and valence interacted in attentional bias scores with an attentional bias toward positive emotion-laden words rather than positive emotion-label words and that this bias was derived from the disengagement difficulty in positive emotion-laden words. In addition, an attentional bias toward negative emotion-label words relative to positive emotion-label words was observed. The event-related potential (ERP) data demonstrated an interaction between emotional word type, valence, and hemisphere. A significant hemisphere effect was observed during the processing of positive emotion-laden word pairs rather than positive emotion-label, negative emotion-label, and negative emotion-laden word pairs, with positive emotion-laden word pairs eliciting an enhanced P1 in the right hemisphere as compared to the left hemisphere. Our results found a dynamic attentional bias toward positive emotion-laden words; individuals allocated more attention to positive emotion-laden words in the early processing stage and had difficulty disengaging attention from them in the late processing stage.
Multi-type RFC1 repeat expansions as the most common cause of hereditary sensory and autonomic neuropathy

Non-coding repeat expansions within RFC1 and NOTCH2NLC genes have lately been linked to multisystem neurodegenerative diseases, which also shed light on yet undiagnosed patients with inherited peripheral neuropathies. The aim of this study was to identify the genetic basis of patients with hereditary sensory and autonomic neuropathy (HSAN). We collected 79 unrelated DNA samples clinically suspected with HSAN from multiple regions of Japan. Mutation screening was first performed using gene panel sequencing and whole-exome sequencing. Pathogenic/likely pathogenic variants were identified from genes of WNK1/HSN2 (6 cases), SCN9A (3 cases), NTRK1 (3 cases), and DNMT1 (2 cases). Subsequently, long-range flanking PCR and repeat-primed PCR were applied to analyze repeat expansions in RFC1 and NOTCH2NLC. Bi-allelic RFC1 repeat expansions were detected from 20 adult-onset HSAN patients, consisting of [(AAGGG)exp/(AAGGG)exp] (8 cases), [(ACAGG)exp/(ACAGG)exp] (8 cases), and [(AAGGG)exp/(ACAGG)exp] (4 cases). GGC repeat expansion in NOTCH2NLC was found in 1 case. Single-nucleotide variant-based haplotype analysis of patients harboring disease-associated repeat expansions in RFC1 revealed distinguishable haplotypes among subgroups with different repeat genotypes. These findings substantially redefine the genetic spectrum of HSAN, where multi-type RFC1 repeat expansions account for 25.3% of all patients, highlighting the necessity of genetic screening, particularly for adult-onset patients.
Microbial succession during the degradation of bioplastic in coastal marine sediment favors sulfate reducing microorganisms

Marine environments are sinks for many contaminants, including petroleum-based plastic waste. Bioplastics, or biodegradable plastics derived from renewable resources, are considered promising alternatives as numerous studies have demonstrated their degradation in marine environments. However, their rates of degradation vary and microbial consortia responsible for its degradation are not well characterized. Previous research by our group has shown that polyhydroxyalkanoate (PHA) stimulates sulfate reducing microorganisms (SRM), enriches sulfate reduction gene pools, and accumulates antibiotic and metal resistance genes. Here, we quantify the degradation rate of PHA pellets in marine sediment and present the long-term temporal changes in PHA-associated SRM communities over 424 days. For comparative purposes, polyethylene terephthalate (PET) and ceramic served as biofilm controls and the free-living microorganisms in the overlying water column served as a non-biofilm control. PHA experienced a 51% mass loss after 424 days and a generalized additive mixed model predicted that 100% mass loss would require 909 days. Throughout the course of the 424-day exposure, PHA was colonized by a distinct microbial community while PET and ceramic were colonized by similarly structured communities. SRM comprised a larger proportion of the overall community (25 – 40%) in PHA-associated biofilms as compared to PET and ceramic controls across all timepoints. Further, the diversity of SRM was greater within PHA biofilms than PET and ceramic biofilms. This study shows that PHA degrades relatively slowly and promotes a long-term shift in microbial community structure toward sulfate reduction, demonstrating the ability of this manufactured polymer to alter its environment via the disruption of biogeochemical cycling, indicating that PHA rises to the level of pollutant in benthic marine systems.