Miracle-dz/UniFAR-FTUs huggingface dataset:
A Qualitative Study on the Social Determinants of HIV Treatment Engagement Among Black Older Women Living With HIV in the Southeastern United States

Supplemental Digital Content is Available in the Text. Abstract Black older women living with HIV (BOWLH) in the United States are disproportionately affected by HIV infection and poor treatment engagement rates, often caused by multiple social determinants of health. In this descriptive qualitative study, we interviewed 17 BOWLH to investigate the facilitators and barriers to HIV treatment engagement. Data were analyzed using the socioecological framework. Findings demonstrate the positive influences of supportive social networks, perceived benefits, HIV-related knowledge, raising HIV awareness in communities, and impact of HIV state laws. The highlighted barriers were mainly low income, substance use, HIV-related stigma, influence of stereotypes and assumptions about older women living with HIV, and health insurance. Religion, managing comorbidities, attitude toward, HIV disclosure, and caregiving roles had both positive and negative influences on engagement. These findings illuminate factors of HIV treatment engagement that might be culturally founded; disseminating these factors to health care professionals is a critical intervention to support this population.
Effects of Multisession Prefrontal Transcranial Direct Current Stimulation on Long-term Memory and Working Memory in Older Adults

Abstract Transcranial direct current stimulation (tDCS) is a noninvasive form of electrical brain stimulation popularly used to augment the effects of working memory (WM) training. Although success has been mixed, some studies report enhancements in WM performance persisting days, weeks, or even months that are actually more reminiscent of consolidation effects typically observed in the long-term memory (LTM) domain, rather than WM improvements per se. Although tDCS has been often reported to enhance both WM and LTM, these effects have never been directly compared within the same study. However, given their considerable neural and behavioral overlap, this is a timely comparison to make. This study reports results from a multisession intervention in older adults comparing active and sham tDCS over the left dorsolateral pFC during training on both an n-back WM task and a word learning LTM task. We found strong and robust effects on LTM, but mixed effects on WM that only emerged for those with lower baseline ability. Importantly, mediation analyses showed an indirect effect of tDCS on WM that was mediated by improvements in consolidation. We conclude that tDCS over the left dorsolateral pFC can be used as an effective intervention to foster long-term learning and memory consolidation in aging, which can manifest in performance improvements across multiple memory domains.
Morpho-functional comparison of differentiation protocols to create iPSC-derived cardiomyocytes

ABSTRACT Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) offer an attractive platform for cardiovascular research. Patient-specific iPSC-CMs are very useful for studying disease development, and bear potential for disease diagnostics, prognosis evaluation and development of personalized treatment. Several monolayer-based serum-free protocols have been described for the differentiation of iPSCs into cardiomyocytes, but data on their performance are scarce. In this study, we evaluated two protocols that are based on temporal modulation of the Wnt/β-catenin pathway for iPSC-CM differentiation from four iPSC lines, including two control individuals and two patients carrying an SCN5A mutation. The SCN5A gene encodes the cardiac voltage-gated sodium channel (Nav1.5) and loss-of-function mutations can cause the cardiac arrhythmia Brugada syndrome. We performed molecular characterization of the obtained iPSC-CMs by immunostaining for cardiac specific markers and by expression analysis of selected cardiac structural and ionic channel protein-encoding genes with qPCR. We also investigated cell growth morphology, contractility and survival of the iPSC-CMs after dissociation. Finally, we performed electrophysiological characterization of the cells, focusing on the action potential (AP) and calcium transient (CT) characteristics using patch-clamping and optical imaging, respectively. Based on our comprehensive morpho-functional analysis, we concluded that both tested protocols result in a high percentage of contracting CMs. Moreover, they showed acceptable survival and cell quality after dissociation (>50% of cells with a smooth cell membrane, possible to seal during patch-clamping). Both protocols generated cells presenting with typical iPSC-CM AP and CT characteristics, although one protocol (that involves sequential addition of CHIR99021 and Wnt-C59) rendered iPSC-CMs, which were more accessible for patch-clamp and calcium transient experiments and showed an expression pattern of cardiac-specific markers more similar to this observed in human heart left ventricle samples.
Survival analysis of clinical and genetic factors in an amyotrophic lateral sclerosis cohort from China

ABSTRACT Objectives To investigate the clinical and genetic factors influencing the survival of amyotrophic lateral sclerosis (ALS) patients in China. Methods Patients were enrolled in the study between December 2013 and December 2018. Clinical variables were recorded upon patient diagnosis. Causative genes related to ALS were screened by whole-exome sequencing and validated by Sanger sequencing. Each patient was followed up every 3–6 months until the endpoint (death or tracheotomy) or the last connection time on 31 December 2020. Propensity score matching analysis was performed to match the genetic and non-genetic ALS patients. The Kaplan–Meier method and multivariable Cox regression were performed for survival analysis. Results A total of 337 patients, including 32 with genetic ALS and 305 with non-genetic ALS, were enrolled in the study. Before matching, in univariate analysis, age of onset (P < 0.001), site of onset (P = 0.036), diagnostic delay (P < 0.001), ALSFRS-R score at diagnosis (P < 0.001), ΔALSFRS-R (P < 0.001), and causative mutations (P = 0.020) were significant prognostic factors. These factors remained statistically significant after multivariate analysis. After matching, in the multivariate analysis, age of onset (P = 0.003), site of onset (P = 0.014), diagnostic delay (P = 0.007), ALSFRS-R score at diagnosis (P = 0.010), ΔALSFRS-R (P = 0.007), and causative mutations (P = 0.003) were found to be significant prognostic factors. Conclusion Both clinical factors and genetic factors influenced survival in our ALS cohort. Clarifying of the underlying mechanisms is crucial for the development of future therapies.
The time course of reactivity to social acceptance and rejection feedback: An examination of event-related potentials and behavioral measures in a peer interaction task.

Measurement of social processes is of interest across areas of research. Event-related potentials (ERPs) measured using electroencephalography (EEG) offer high temporal resolution, but little work has leveraged this strength to characterize the time course of social feedback processing. The present study aimed to replicate and extend previous research by systematically identifying the temporal dynamics of responses to both social acceptance and rejection feedback relative to neutral feedback, examining internal consistency of ERPs, and exploring correspondence with behavioral measures elicited during a peer interaction task. Emerging adults (N = 118) completed a computerized peer interaction task in which they made decisions to accept or reject peers and received rejection, acceptance, and neutral feedback while EEG data were recorded. Principal component analysis was used to derive temporally and spatially distinct ERP components sensitive to positive and negative social feedback. Participant voting patterns and post-task liking ratings for computer-controlled peers were also examined. Replicating prior work, components consistent with N1, P2, reward positivity (RewP), and P3 emerged, but distinct patterns of modulation by acceptance and rejection relative to neutral feedback were observed. Most components showed acceptable internal consistency. Sensitivity to peer feedback assessed through participant voting patterns and liking ratings for peers was correlated with RewP and P3 components. Results highlight the complexity of social feedback processing observable in a computerized peer interaction task and offer promising neural and behavioral measures that can be used to examine individual differences in sensitivity to both social acceptance and rejection feedback.
Interobserver variation in the interpretation of magnetic resonance enterography in Crohn's disease.

OBJECTIVES To evaluate inter observer variability for diagnosis of disease presence and extent of small bowel and colonic Crohn's disease using MR enterography (MRE). METHODS Data from the first 73 consecutive patients (mean age 32, 33F, 28 new diagnosis, 45 suspected relapse) recruited to a multicentre, prospective diagnostic accuracy trial evaluating MRE for small bowel Crohn's disease were each read independently by three (from a pool of twenty) radiologists. Radiologists documented presence and segmental location of small bowel Crohn's disease and recorded morphological mural/extra mural parameters for involved segments. Per patient percentage agreement for disease presence and extent were calculated against an outcome-based construct reference standard was calculated (averaged between pairs of readers). Prevalence adjusted bias adjusted κ (PABAK) was calculated. RESULTS Agreement for small bowel disease presence for new diagnosis/relapsed patients was 68%(κ = 0.36)/ 78% (κ = 0.56) and for disease extent 43%(κ = 0.14)/ 53% for disease extent (κ = 0.07), respectively. For disease presence all three radiologists agreed correctly with the reference standard in 41/59 (69%) of patients with small bowel involvement, and in 8/14 (57%) cases of disease absence. Agreement was highest for multi segment disease, greater than 5 cm in length, with mural thickness >6 mm, and increased mural T2 signal. Agreement for colonic disease presence was 61% (κ = 0.21 fair agreement) for new diagnosis/ 60% (κ = 0.20, slight agreement) for relapsed patients. CONCLUSION There is reasonable agreement between radiologists for small bowel disease presence using MRE for newly diagnosed Crohn's disease, and patients with suspected relapse respectively. Agreement is lower for disease extent. ADVANCES IN KNOWLEDGE There is reasonable agreement between radiologists for small bowel disease presence using MRE for newly diagnosed (68%) Crohn's disease, and patients with suspected relapse (78%). Agreement is lower for disease extent (43% new diagnosis and 53% suspected relapse).
Elevated shear rate-induced by exercise increases eNOS ser1177 but not PECAM-1 Tyr713 phosphorylation in human conduit artery endothelial cells

ABSTRACT Although evidence demonstrates the fundamental role of shear stress in vascular health, predominantly through the release of nitric oxide (NO), the mechanisms by which endothelial cells (EC)s sense and transduce shear are poorly understood. In cultured ECs tyrosine phosphorylation of PECAM-1 has been shown to activate eNOS in response to shear stress. However, in the human skeletal muscle microcirculation PECAM-1 was not activated in response to exercise or passive leg movement. Given this contradiction, this study aimed to assess the effect of exercise on conduit artery PECAM-1 and eNOS activation in humans. Eleven males were randomised to two groups; 30 min of handgrip exercise (n = 6), or a time-control group (n = 5). Protein content of eNOS and PECAM-1, alongside eNOS Ser1177 and PECAM-1 Tyr713 phosphorylation were assessed in ECs obtained from the radial artery pre- and post-intervention. Handgrip exercise resulted in a 5-fold increase in mean shear rate in the exercise group, with no change in the control group (group*time, P < 0.001). There was a 54% increase in eNOS Ser1177 phosphorylation in the exercise group, when compared to control group (group*time, P = 0.016), but no change was reported in PECAM-1 Tyr713 phosphorylation in either group (group*time, P > 0.05). eNOS and PECAM-1 protein content were unchanged (group*time, P > 0.05). Our data show that exercise-induced elevations in conduit artery shear rate increase eNOS Ser1177 phosphorylation but not PECAM-1 Tyr713 phosphorylation. This suggests PECAM-1 phosphorylation may not be involved in the vascular response to acute but prolonged elevations in exercise-induced shear rate in conduit arteries of healthy, active men.
Effects of Chronic Burden Across Multiple Domains and Experiences of Daily Stressors on Negative Affect.

BACKGROUND Exposure to chronic psychological stress across multiple life domains (multi-domain chronic burden) is associated with poor health. This may be because multi-domain chronic burden influences daily-level emotional processes, though this hypothesis has not been thoroughly tested. PURPOSE The current study tested whether (a) multi-domain chronic burden is associated with greater exposure to daily stressors and (b) multi-domain chronic burden compounds negative affect on days with stressors compared to stressor-free days. METHODS The MIDUS Study (Wave II) and the National Study of Daily Experiences sub-study were conducted from 2004 to 2006 (N = 2,022). Participants reported on eight life domains of psychological stress used to create a multi-domain chronic burden summary score. For eight consecutive days, participants reported the daily occurrence of stressful events and daily negative affect. RESULTS Participants with greater multi-domain chronic burden were significantly more likely to report daily stressors. There was also a significant interaction between multi-domain chronic burden and daily stressors on negative affect: participants with higher multi-domain chronic burden had greater negative affect on stressor days than stressor-free days compared to those with lower multi-domain chronic burden. CONCLUSION Participants with higher multi-domain chronic burden were more likely to report daily stressors and there was a compounding effect of multi-domain chronic burden and daily stressors on negative affect. These results suggest that experiencing a greater amount of psychological stress across multiple life domains may make daily stressors more toxic for daily affect.
Multilevel analysis to identify the factors associated with caesarean section in Bangladesh: evidence from a nationally representative survey

Abstract Background Caesarean delivery has a significant role in reducing maternal and child death. However, unnecessary utilization has adverse health effects. This study aimed to assess the prevalence and associated factors of caesarean delivery in Bangladesh. Methods Data from the latest Bangladesh Multiple Indicator Cluster Survey (MICS, 2019) was used in this study. Since MICS data are hierarchical in nature, multilevel modelling was used. Results The prevalence of caesarean section (CS) was 67.4% among Bangladeshi women. Multilevel analysis suggests the age of the women, household wealth status, utilization of antenatal care (ANC) , delivery at a health facility and division were significantly associated with CS. Women who delivered in a private health facility had the highest odds for CS (odds ratio [OR] 10.35 [95% confidence interval {CI} 8.55 to 12.54]). Women 30–34 y of age had a 36% higher likelihood of CS compared with women 15–19 y of age (OR 1.36 [95% CI 1.03 to 1.79]). The odds of CS positively increased with household wealth status. Women who had at least one ANC visit had a 1.7 times higher possibility of CS (OR 1.70 [95% CI 1.26 to 2.30]). Conclusions Policy guidelines on caesarean deliveries are urgently needed in Bangladesh to avoid unnecessary caesarean deliveries and protect mothers from the consequences.
The effect of mental countermeasures on a novel brain‐based feedback concealed information test

The feedback concealed information test (fCIT) is a novel form of the CIT, providing participants with feedback regarding their memory concealment performance. The fCIT utilizes event‐related potentials (recognition‐P300 and feedback‐related event‐related potentials) and has been shown to provide high efficiency in detecting information concealment. However, it is unclear how well the fCIT performs in the presence of mental countermeasures. To address this question, participants were trained to use countermeasures during fCIT. Results showed that the recognition‐P300 efficiency decreased when participants used countermeasures. However, the efficiencies of feedback‐related negativity and feedback‐P300 were unchanged, with feedback‐P300 still showing a high detection efficiency (AUC = 0.86) during countermeasures. These findings demonstrate the potential of fCIT for subverting countermeasures.
High-Risk Human Papillomavirus Messenger RNA Testing Using Urine, Cervicovaginal Self-Collected and Provider-Collected Cervical Samples Among Women in Mombasa, Kenya

Urine sampling to test for high-risk human papillomavirus infection is a noninvasive method of cervical cancer screening. Although most women found urine sampling comfortable, it was less likely to detect high-grade cervical lesions than self- and provider-collected cervical sampling. Abstract We compared human papillomavirus messenger RNA testing using urine, self-, and provider-collected samples for the detection of high-grade cervical cytology and assessed acceptability of urine self-collection among females who engage in sex work in Kenya. Participants found urine sampling comfortable, but high-risk human papillomavirus messenger RNA detection in urine samples was less likely to detect high-grade lesions than self- and provider-collected cervical samples.
Human lymph node immune dynamics as driver of vaccine efficacy: an understudied aspect of immune responses

ABSTRACT Introduction During the last century, changes in hygiene, sanitation, and the advent of childhood vaccination have resulted in profound reductions in mortality from infectious diseases. Despite this success, infectious diseases remain an enigmatic public health threat, where effective vaccines for influenza, human immunodeficiency virus (HIV), tuberculosis, and malaria, among others remain elusive. Area covered In addition to the immune evasion tactics employed by complex pathogens, our understanding of immunopathogenesis and the development of effective vaccines is also complexified by the inherent variability of human immune responses. Lymph nodes (LNs) are the anatomical sites where B cell responses develop. An important, but understudied component of immune response complexity is variation in LN immune dynamics and in particular variation in germinal center follicular helper T cells (Tfh) and B cells which can be impacted by genetic variation, aging, the microbiome and chronic infection. Expert opinion We describe the contribution of genetic variation, aging, microbiome and chronic infection on LN immune dynamics and associated Tfh responses and offers perspective on how inclusion of LN immune subset and cytoarchitecture analyses, along with peripheral blood biomarkers can supplement systems vaccinology or immunology approaches for the development of vaccines or other interventions to prevent infectious diseases.
COVID‐DAI: A novel framework for COVID‐19 detection and infection growth estimation using computed tomography images

The COVID‐19 pandemic is spreading at a fast pace around the world and has a high mortality rate. Since there is no proper treatment of COVID‐19 and its multiple variants, for example, Alpha, Beta, Gamma, and Delta, being more infectious in nature are affecting millions of people, further complicates the detection process, so, victims are at the risk of death. However, timely and accurate diagnosis of this deadly virus can not only save the patients from life loss but can also prevent them from the complex treatment procedures. Accurate segmentation and classification of COVID‐19 is a tedious job due to the extensive variations in its shape and similarity with other diseases like Pneumonia. Furthermore, the existing techniques have hardly focused on the infection growth estimation over time which can assist the doctors to better analyze the condition of COVID‐19‐affected patients. In this work, we tried to overcome the shortcomings of existing studies by proposing a model capable of segmenting, classifying the COVID‐19 from computed tomography images, and predicting its behavior over a certain period. The framework comprises four main steps: (i) data preparation, (ii) segmentation, (iii) infection growth estimation, and (iv) classification. After performing the pre‐processing step, we introduced the DenseNet‐77 based UNET approach. Initially, the DenseNet‐77 is used at the Encoder module of the UNET model to calculate the deep keypoints which are later segmented to show the coronavirus region. Then, the infection growth estimation of COVID‐19 per patient is estimated using the blob analysis. Finally, we employed the DenseNet‐77 framework as an end‐to‐end network to classify the input images into three classes namely healthy, COVID‐19‐affected, and pneumonia images. We evaluated the proposed model over the COVID‐19‐20 and COVIDx CT‐2A datasets for segmentation and classification tasks, respectively. Furthermore, unlike existing techniques, we performed a cross‐dataset evaluation to show the generalization ability of our method. The quantitative and qualitative evaluation confirms that our method is robust to both COVID‐19 segmentation and classification and can accurately predict the infection growth in a certain time frame.