Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Temperature and Growth Regulators on Formation of Anlagen, Tendrils and Inflorescences in Vitis vinifera L.

The effects of single, combined and sequential application of gibberellic acid (GA), chlormequat and cytokinins on the formation of anlagen, tendrils and inflorescences were studied in grapevines (cv. Muscat of Alexandria) grown with natural illumination at high temperature (30 °C day to 25 °C night) and at low temperature (21 °C day to 16°C night or 18 °C day to 13 °C night). GA promoted the formation of anlagen and growth of tendrils regardless of temperature, but inhibited inflorescence production. Chlormequat had the opposite effect on anlagen formation and tendril growth and promoted inflorescence formation from pre-formed anlagen or from tendril initials. While low temperature is normally unfavourable for inflorescence formation, this was induced by chlormequat even at low temperature, but only with summer light conditions. Cytokinin application to plants pre-treated with chlormequat caused tendrils to grow into inflorescences regardless of temperature regimes. Moreover, shoot primordia were also formed in place8of tendrils in cytokinin treated plants even without chlormequat pre-treatment.
Efficacy and safety of two-drug regimens for treatment of HIV in the central nervous system.

HIV can infect the central nervous system (CNS) soon after transmission, and early initiation of antiretroviral therapy (ART) reduces the establishment or persistence of viral reservoirs in the CNS compartment as well as HIVassociated CNS injury [1,2]. Successful treatment and virus eradication will require approaches that are effective, well tolerated and durable in the CNS [3,4]. Despite current ART regimens that suppress HIV RNA in blood to undetectable levels, people with HIV (PWH) differ substantially in HIV RNA concentrations in cerebrospinal fluid (CSF) [5–8]. In most PWH, HIV RNA concentrations in CSF are more than 1 log10 copies/ml lower in CSF than in blood. After initiation of ART, viral suppression in blood is the primary determinant of viral suppression in CSF [5,7]. During therapy, a minority of PWH have detectable HIV RNA in CSF when HIV RNA in blood is undetectable, a condition termed CSF viral escape that can occur with or without symptoms [6,9–12]. CSF viral escape is associated with ART drugs that do not achieve therapeutic concentrations in the CNS and/or have reduced efficacy as a consequence of drug resistance mutations, among other factors such as worse immune status [6,8,13–15]. Newer ART drugs with higher potency, a high genetic barrier to resistance and a longer half-life, such as second-generation integrase strand transfer inhibitors (INSTIs), are more effective at suppressing HIV systemically and typically also do so in the CNS. These attributes have led to efforts to simplify ART regimens to reduce cost, improve medication adherence and limit toxicities. However, concerns exist that simplification approaches could lead to HIVescape in the CNS or other tissues wherein drug distribution and
Novel phospho-switch function of delta-catenin in dendrite development

Baumert et al. identify a novel “phospho-switch” within the neuronal protein delta-catenin. This phospho-switch, located at delta-catenin’s C-terminus, is sensitive to upstream glutamate signaling and instructs dendrites to branch versus extend during development by determining delta-catenin’s neuronal binding partners and subsequent modulation of actin-associated proteins.
3D Stem Cell Culture

Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seeking optimal stem cell preparations for basic research and clinical applications, and these might be attainable through 3D culture of stem cells. The 3D cultures recapitulate the in vivo cell-to-cell and cell-to-matrix interactions more effectively, and the cells in 3D cultures exhibit many unique and desirable characteristics. The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell preparations for possible novel therapeutic applications.
A Siderophore Analog of Fimsbactin from Acinetobacter Hinders Growth of the Phytopathogen Pseudomonas syringae and Induces Systemic Priming of Immunity in Arabidopsis thaliana

Siderophores produced in soil by plant growth-promoting rhizobacteria (PGPRs) play several roles, including nutrient mobilizers and can be useful as plants defense elicitors. We investigated the role of a synthetic mixed ligand bis-catechol-mono-hydroxamate siderophore (SID) that mimics the chemical structure of a natural siderophore, fimsbactin, produced by Acinetobacter spp. in the resistance against the phytopathogen Pseudomonas syringae pv tomato DC3000 (Pst DC3000), in Arabidopsis thaliana. We first tested the antibacterial activity of SID against Pst DC3000 in vitro. After confirming that SID had antibacterial activity against Pst DC3000, we tested whether the observed in vitro activity could translate into resistance of Arabidopsis to Pst DC3000, using bacterial loads as endpoints in a plant infection model. Furthermore, using quantitative polymerase chain reaction, we explored the molecular actors involved in the resistance of Arabidopsis induced by SID. Finally, to assure that SID would not interfere with PGPRs, we tested in vitro the influence of SID on the growth of a reference PGPR, Bacillus subtilis. We report here that SID is an antibacterial agent as well as an inducer of systemic priming of resistance in A. thaliana against Pst DC3000, and that SID can, at the same time, promote growth of a PGPR.
A work-family enrichment intervention: Transferring resources across life domains.

This study expands the work-family enrichment literature by integrating enrichment theory (Greenhaus & Powell, 2006), social-cognitive theory (Bandura, 2001), capitalization theory (e.g., Gable, Reis, Impett, & Asher, 2004), and creative cognition theory (e.g., Smith, Ward, & Finke, 1995), in order to advance a novel conceptual model of the effects of resource transfer training on enrichment and job satisfaction. The model was tested by developing and evaluating a work-to-family enrichment training program, the Resource Transfer Training, which was based on Greenhaus and Powell's (2006) enrichment theory and interpersonal capitalization theory (Ilies, Keeney, & Scott, 2011). The training was administered to 163 administrative employees using a longitudinal prepost experimental design. Consistent with hypotheses, findings indicated that, compared to a control training condition, the Resource Transfer Training increased development-based enrichment from work to family (the transfer of skills, knowledge and values). Furthermore, the effect of the training on both development-based and affect-based enrichment (the transfer of positive affect) was mediated by enrichment self-efficacy, and the indirect effects of the training on enrichment were moderated by initial social-emotional work resources. Finally, as hypothesized, enrichment self-efficacy and enrichment experiences sequentially mediated the effect of the training on job satisfaction. This work advances theory by demonstrating that human agency can facilitate enrichment experiences across roles, and by exploring new antecedents of enrichment through theoretically driven training components. It advances practice by devising and testing a work-to-family enrichment intervention that can serve as a tool for organizations to increase positive synergy between work and nonwork roles. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Increasing HIV prevalence and injection drug use among men who have sex with men in Ho Chi Minh City, Vietnam

Vietnam has been conducting HIV/sexually transmitted infection (STI) integrated bio-behavioral surveillance surveys on men who have sex with men (MSM) as well as other key populations since 2005. Although HIV prevalence in the Vietnamese general population remains below 1%, it is expected to be much higher among MSM. Data on HIV prevalence and sexual and drug use behaviors were collected from MSM in Ho Chi Minh City (HCMC) in 2006 (n = 397), 2009 (n = 399) and 2013 (n = 350) using respondent-driven sampling. Eligible participants were males, aged ≥15 years who reported having manual, oral, or anal sexual activity with males in the past year and lived, worked or socialized in HCMC. HIV seroprevalence among MSM was 5.8% in 2006, 16.1% in 2009 and 12.1% in 2013 and prevalence of at least one STI (syphilis, gonorrhea and/or chlamydia infection) was 11.4% in 2006 and 15.6% in 2009 (no data for 2013). Significant, but small, increasing trends were found for MSM who reported ever testing and receiving results for HIV and for HIV prevalence. No significant changes for condom use, injecting and non-injecting drug use, or and receipt of free condoms were observed. Although a small percentage of MSM reported injecting drugs, HIV was positively associated with ever injecting drugs. Programs targeting MSM should include screening and treatment for injection drug use to most effectively control the HIV/AIDS epidemic among MSM in HCMC.
Physician Work Hours and the Gender Pay Gap - Evidence from Primary Care.

BACKGROUND The gender gap in physician pay is often attributed in part to women working fewer hours than men, but evidence to date is limited by self-report and a lack of detail regarding clinical revenue and gender differences in practice style. METHODS Using national all-payer claims and data from electronic health records, we conducted a cross-sectional analysis of 24.4 million primary care office visits in 2017 and performed comparisons between female and male physicians in the same practices. Our primary independent variable was physician gender; outcomes included visit revenue, visit counts, days worked, and observed visit time (interval between the initiation and the termination of a visit). We created multivariable regression models at the year, day, and visit level after adjustment for characteristics of the primary care physicians (PCPs), patients, and types of visit and for practice fixed effects. RESULTS In 2017, female PCPs generated 10.9% less revenue from office visits than their male counterparts (-$39,143.2; 95% confidence interval [CI], -53,523.0 to -24,763.4) and conducted 10.8% fewer visits (-330.5 visits; 95% CI, -406.6 to -254.3) over 2.6% fewer clinical days (-5.3 days; 95% CI, -7.7 to -3.0), after adjustment for age, academic degree, specialty, and number of sessions worked per week, yet spent 2.6% more observed time in visits that year than their male counterparts (1201.3 minutes; 95% CI, 184.7 to 2218.0). Per visit, after adjustment for PCP, patient, and visit characteristics, female PCPs generated equal revenue but spent 15.7% more time with a patient (2.4 minutes; 95% CI, 2.1 to 2.6). These results were consistent in subgroup analyses according to the gender and health status of the patients and the type and complexity of the visits. CONCLUSIONS Female PCPs generated less visit revenue than male colleagues in the same practices owing to a lower volume of visits, yet spent more time in direct patient care per visit, per day, and per year. (Funded in part by the Robert Wood Johnson Foundation.).
Regular use of proton pump inhibitors and risk of type 2 diabetes: results from three prospective cohort studies

Objective The association between the regular use of proton pump inhibitors (PPIs) and the risk of type 2 diabetes remains unclear, although a recent randomised controlled trial showed a trend towards increased risk. This study was undertaken to evaluate the regular use of PPIs and risk of type 2 diabetes. Method This is a prospective analysis of 204 689 participants free of diabetes in the Nurses' Health Study (NHS), NHS II and Health Professionals Follow-up Study (HPFS). Type 2 diabetes was confirmed using American Diabetes Association (ADA) diagnostic criteria. We evaluated hazard ratios (HRs) adjusting for demographic factors, lifestyle habits, the presence of comorbidities, use of other medications and clinical indications. Results We documented 10 105 incident cases of diabetes over 2 127 471 person-years of follow-up. Regular PPI users had a 24% higher risk of diabetes than non-users (HR 1.24, 95% CI 1.17 to 1.31). The risk of diabetes increased with duration of PPI use. Fully adjusted HRs were 1.05 (95% CI 0.93 to 1.19) for participants who used PPIs for >0–2 years and 1.26 (95% CI 1.18 to 1.35) for participants who used PPIs for >2 years compared with non-users. Conclusions Regular use of PPIs was associated with a higher risk of type 2 diabetes and the risk increased with longer duration of use. Physicians should therefore exercise caution when prescribing PPIs, particularly for long-term use.
Endocrine and metabolic responses to glucose, insulin, and adrenocorticotropin (ACTH) infusions in early-lactation dairy goats of high and low milk yield.

This experiment aimed to examine endocrine and metabolic responses to glucose, insulin, and adrenocorticotropin (ACTH) infusions in early-lactation dairy goats of different levels of milk production (LMP). Goats were grouped as either high (HY; 4.0 L/d, n = 13) or low milk yield (LY; 2.4 L/d, n = 13). Individual milk yield (L/d) and dry matter intake (DMI; kg/d) were measured daily. Concentration (mM) of glucose, fatty acids, and β-hydroxybutyrate, percent of milk fat and protein, body weight (BW; kg), and body condition score (BCS) were assessed weekly (from 2-6 wk postpartum). An intravenous glucose tolerance test (IVGTT), an insulin tolerance test (ITT), and an ACTH stimulation test were carried out at 43, 44, and 45 ± 0.7 d in milk, respectively. The HY goats had greater milk yield (+67%), energy-corrected milk (ECM; +70%), DMI (+28%), ratio of ECM output to metabolic BW (+67%), and feed efficiency (+25%), but lesser BCS than LY goats (2.4 vs. 2.6). The DMI (% of BW) was moderately correlated with ECM (r = 0.70) and negatively correlated with BCS (r = -0.57). At the time of the IVGTT, HY goats had lesser basal insulin and glucose than LY goats. However, results from IVGTT and ITT indicate that the sensitivity of peripheral tissues to insulin was unaffected by LMP. Compared with LY, HY goats had lesser insulin secretion (-52%) and greater insulin clearance rate (+47%) after glucose infusion. The ITT and ACTH stimulation test results show that both the growth hormone response to insulin and the cortisol response to ACTH were unaffected by LMP. Also, basal plasma concentrations of GH and cortisol were not correlated with glucose and fatty acids concentrations or any performance traits. Collectively, our results suggest that differences between HY and LY goats, concerning milk yield and feed efficiency, were probably more closely related to differences in insulin secretion and clearance than to differences in peripheral tissue responsiveness to the effects of catabolic and anabolic hormones.
Knockdown of NEAT1 mitigates ox-LDL-induced injury in human umbilical vein endothelial cells via miR-30c-5p/TCF7 axis.

OBJECTIVE Atherosclerosis is an inflammation-associated disease resulting in a huge health hazard. Abundance of researches showed that long non-coding RNAs (lncRNAs) played vital roles in atherosclerosis, but the molecular mechanism of nuclear-enriched abundant transcript (NEAT1) has not been fully elucidated yet. PATIENTS AND METHODS Human umbilical vein endothelial cells (HUVECs) were treated with oxidized low-density lipoprotein (ox-LDL) for constructing the model of atherosclerosis. The detection of NEAT1, microRNA-30c-5p (miR-30c-5p), and transcription factor 7 (TCF7) expression was implemented by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cell proliferation and apoptosis were measured by 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) and flow cytometry, respectively. The levels of apoptosis-associated proteins were examined through Western blot and the concentrations of inflammatory cytokines were determined by enzyme-linked immunosorbent assay (ELISA). The targeted relationship was analyzed by Dual-Luciferase reporter assay. RESULTS NEAT1 was upregulated in serum of patients with atherosclerosis and HUVECs treated with ox-LDL. Knockdown of NEAT1 exerted the promotion of proliferation but suppression of apoptosis and inflammation in ox-LDL-treated HUVECs. Moreover, NEAT1 targeted miR-30c-5p and the overexpression of miR-30c-5p reversed the ox-LDL-induced effects in HUVECs. Furthermore, miR-30c-5p directly refrained the TCF7 level, and NEAT1 repression decreased the expression of TCF7 by upregulating miR-30c-5p. The knockdown of NEAT1 afforded the protective effect for HUVECs treated with ox-LDL through miR-30c-5p/TCF7 axis. CONCLUSIONS The knockdown of NEAT1 overtly motivated proliferation but alleviated the apoptosis and inflammation in ox-LDL-treated HUVECs by miR-30c-5p/TCF7 axis. NEAT1 accelerated the progression of atherosclerosis therapies, functioning as an indicative element.
COMPARISON OF THE MORTALITY RESPONSE OF DIFFERENT MAMMALIAN SPECIES TO X- RAYS AND FAST NEUTRONS

Difficulties encountered in determining the relative biological effects of x, gamma , and neutron radiations for various mammalian species are discussed. Pertinent data are reviewed on the effects of x, gamma , and fast neutron radiations in mice, rats, and dogs. Data are included on the mortality response to heavy particle radiation from the B/sup 10/(n, alpha )Li/sup 7/ reaction. No biophysical explanations were found for species differences in response to radiation. The data indicate a difference in the relative radiosensitivity of the intestine and bone marrow for x, gamma , and neutron radiation and also indicate that this radiosensitivity is highly species dependent. (C.H.)
Dry needling as a novel intervention for cervicogenic somatosensory tinnitus: a case study

ABSTRACT Tinnitus is defined as conscious perception of sound in the absence of a corresponding external stimulus. A condition that affects 10 − 15% of the adult population, tinnitus may be caused by an interaction between the somatosensory and auditory system, more formally known as somatosensory tinnitus. Cervicogenic somatosensory tinnitus is a subgroup of somatosensory tinnitus involving anatomical structures and physiological mechanisms associated with the cervical spine. A limited number of studies have reported inconsistent outcomes for treating cervicogenic somatosensory tinnitus with conservative treatment strategies such as manual therapy and exercise. However, dry needling is a skilled, manual therapy intervention that has recently gained popularity among the physical therapy profession that may be useful for both evaluating and treating the condition. The following case report describes the use of dry needling to evaluate and treat a patient with cervical somatosensory tinnitus and concurrent cervicogenic headaches. Physical therapy that targeted the muscles of the upper cervical spine with dry needling resulted in a meaningful reduction in cervicogenic somatosensory tinnitus, and the improvements persisted at 1-year follow-up. Further research, including randomized control trials, is warranted to fully determine the potential of dry needling to treat cervicogenic somatosensory tinnitus.
Group-Based Emotions Over Time: Dynamics of Experience and Regulation

People’s emotions toward their ingroups and salient outgroups often change over time as a result of changing circumstances or intentional self-regulation. To investigate such dynamics, two studies assessed participants’ perceived past, present, and ideal levels of group-based emotions toward ingroups and outgroups, for several different types of groups. Consistent with predictions, participants ideally wanted to feel more positive and less negative emotions toward the ingroup compared to their present levels. However, contrary to predictions, ideal emotions toward competitive outgroups were more positive than negative. Several effects over time suggested the successful regulation of emotion: Ideal levels of positive ingroup emotion predicted group-related behavioral intentions as well as emotions reported at a later time, over and above present levels. This work puts group-based emotions in a subjective temporal context and opens new directions for theory-driven investigation and new possibilities for interventions.
Ultimate Full Contact: Fight Outcome Characterization Concerning Their Methods, Occurrence Times and Technical–Tactical Developments

Fight analysis produces relevant technical–tactical information. However, this knowledge is limited in hybrid full-contact combat sports. Therefore, this study aimed to characterize the results of the fights’ outcomes through the winners at the World Ultimate Full Contact (WUFC) Championships between 2008 and 2017. Methods: 170 combats between senior male fighters (master class) from 38 countries were observed; all fight outcome methods, their occurrence times, inherent skills and their development forms were analyzed through frequencies, percentages, crosstabs and chi-square test, considering a Fisher’s exact value of p < 0.05. The fight outcome methods were, in decreasing order, as follows: submission; decision and technical knockout (TKO); knockout (KO); and doctor stoppage. Only 19.4% fights completed the regular time 10 min (600 s), and 68.8% fight outcomes occurred in the first 5 min (300 s). Chokes were more used than joint locks, primarily developed in single actions. Head punches and kicks were the skills most responsible for KO, developed more in combinations and counter-attacks, while TKO was always through combination attacks and mostly by ground and pound. Ground fighting is most effective. In stand-up fighting, combination attacks and counter-attack are most effective. It is important to increase the technical–tactical capacities and adjustable decision-making to perform the regular fight time.
Patterns of healthcare seeking among people reporting chronic conditions in rural sub‐Saharan Africa: findings from a population‐based study in Burkina Faso

Non‐communicable diseases are rapidly becoming one of the leading causes of morbidity and mortality in sub‐Saharan Africa. Yet, little is known about patterns of healthcare seeking among people with chronic conditions in these settings. We aimed to explore determinants of healthcare seeking among people who reported at least one chronic condition in rural Burkina Faso.