Miracle-dz/UniFAR-FTUs huggingface dataset:
Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis

Acute pancreatitis (AP) is a local and/or systemic inflammatory disease that starts with acinar cell injury and necrosis; it has no effective medical treatment and thus remains a life-threatening condition. Interleukin-37 (IL-37), a natural immunomodulator, has demonstrated an antiinflammatory effect; however, the role of IL-37 in AP remains unknown. The serum IL-37 levels of 39 healthy controls and 94 patients with AP were measured. Cholecystokinin was applied to induce pancreatic acinar cell injury in vitro. Classical experimental AP models, such as caerulein, l-arginine, and taurolithocholic acid 3-sulfate disodium salt, were included in the in vivo study. A transgenic mouse model with the IL-37 gene and administration of recombinant IL-37 were used to further investigate the function of IL-37 in AP. Pancreas-specific gasdermin D–knockout (GSDMD-knockout) mice were used to explore the protective mechanism of IL-37. Our results showed that serum IL-37 levels in humans were negatively correlated with the severity of AP. Furthermore, IL-37–transgenic mice and supplementation with recombinant IL-37 could both protect against AP. Mechanistically, IL-37 was able to suppress pyroptosis of injured acinar cells, and specific depletion of GSDMD in the pancreas counteracted the protective effect of IL-37. Our study demonstrates that IL-37 protects against acinar cell pyroptosis in AP.
Pressure Tuning of Coupled Structural and Spin State Transitions in the Molecular Complex [Fe(H2B(pz)2)2(phen)].

The large volume change, which accompanies the molecular spin crossover (SCO) phenomenon in some transition metal complexes, prompts frequently the coupling of the SCO with other instabilities. Understanding the driving mechanism(s) of such coupled phase transitions is not only important for fundamental reasons but also provides scope for the development of multifunctional materials. The general theoretical expectation is that the coupling has elastic origin, and the sequence of transitions can be tuned by an externally applied pressure, but dedicated experiments remain scarce. Here, we used high-pressure and low-temperature single-crystal X-ray diffraction to investigate the high-spin (HS) to low-spin (LS) transitions in the molecular complexes [FeII(H2B(pz)2)2(bipy)] and [FeII(H2B(pz)2)2(phen)]. In the bipyridine complex, the SCO is continuous and isostructural over the whole T, P-range (100-300 K, 0-2 GPa). In the phenanthroline derivative, however, the SCO is concomitant with a symmetry-breaking transition (C2/c to P1̅). Structural analysis reveals that the coupling between the two phenomena can be tuned by external pressure from a virtually simultaneous HSC2/c-LSP1̅ transition to the sequence of HSC2/c-LSC2/c-LSP1̅ transitions. The correlation of spontaneous strain and order parameter behaviors highlights that the "separated" transitions remain still connected via strain coupling, whereas the "simultaneous" transitions are partially split.
Patient, family members and community pharmacists' views of a proposed overdose prevention intervention delivered in community pharmacies for patients prescribed high-strength opioids for chronic non-cancer pain: An explorative intervention development study.

INTRODUCTION Despite opioid prescribing for chronic non-cancer pain (CNCP) having limited therapeutic benefits, recent evidence indicates significant increases in the prescribing of high-strength opioids for individuals with CNCP. Patients prescribed opioids for CNCP have overdose risk factors but generally have low opioid overdose awareness and low perceptions of risk related to prescribed opioids. Currently, there are few bespoke overdose prevention resources for this group. METHODS This qualitative study investigated views on a naloxone intervention for people prescribed high-strength opioids for CNCP delivered via community pharmacies. The intervention included overdose risk awareness and naloxone training and provision. Interviews were conducted with eight patients, four family members and two community pharmacists. Participants were convenience sampled and recruited through networks within the Scottish pain community. The Framework approach was used to analyse findings. RESULTS All participants had positive attitudes towards the intervention, but patients and family members considered risk of overdose to be very low. Three themes were identified: potential advantages of the intervention; potential barriers to the intervention; and additional suggestions and feedback about the intervention. Advantages included the intervention providing essential overdose information for CNCP patients. Barriers included resource and time pressures within community pharmacies. DISCUSSION AND CONCLUSION While patients had low overdose knowledge and did not see themselves as being at risk of opioid overdose, they were receptive to naloxone use and positive about the proposed intervention. A feasibility trial is merited to further investigate how the intervention would be experienced within community pharmacy settings.
A Tibetan ice core covering the past 1,300 years radiometrically dated with 39Ar

Significance Ice cores from high mountain glaciers outside the polar regions are unique archives of the past climate, but their use is often hampered by the lack of reliable dating, especially in the age range of 100 to 500 anni (a). The extremely rare noble-gas isotope 39Ar is ideally suited for radiometric dating in this age window. Advances in the detection method, atom trap trace analysis, have allowed us to apply 39Ar dating to an ice core from the Tibetan Plateau, yielding a timescale that covers the past 1,300 a. This work enables the use of further ice cores from polar regions for firn studies or from high mountain glaciers for retrieving climate records of the past millenium and beyond.
Potential for perceived failure of stratospheric aerosol injection deployment

Significance Even if aggressive mitigation policies are implemented soon, climate change impacts will worsen in the coming decades. One proposed response is stratospheric aerosol injection (SAI), which would reflect a small amount of the sun’s energy back to space, thereby cooling the planet. This approach is broadly considered to be relatively inexpensive and straightforward to deploy, and global cooling could occur rapidly. However, on regional scales, internal climate variability is likely to dominate over SAI forcing. This means that in the decade after SAI is deployed, many regions of the world could locally experience even higher temperatures. Our study provides conceptual insight for the possible perception of the failure of SAI or other climate mitigation strategies.
Orai1 Inhibitors as Potential Treatments for Pulmonary Arterial Hypertension

Background: Pulmonary arterial hypertension (PAH) is characterized by progressive distal pulmonary artery (PA) obstruction, leading to right ventricular hypertrophy and failure. Exacerbated intracellular calcium (Ca2+) signaling contributes to abnormalities in PA smooth muscle cells (PASMCs), including aberrant proliferation, apoptosis resistance, exacerbated migration, and arterial contractility. Store-operated Ca2+ entry is involved in Ca2+ homeostasis in PASMCs, but its properties in PAH are unclear. Methods: Using a combination of Ca2+ imaging, molecular biology, in vitro, ex vivo, and in vivo approaches, we investigated the roles of the Orai1 SOC channel in PA remodeling in PAH and determined the consequences of pharmacological Orai1 inhibition in vivo using experimental models of pulmonary hypertension (PH). Results: Store-operated Ca2+ entry and Orai1 mRNA and protein were increased in human PASMCs (hPASMCs) from patients with PAH (PAH-hPASMCs). We found that MEK1/2 (mitogen-activated protein kinase kinase 1/2), NFAT (nuclear factor of activated T cells), and NFκB (nuclear factor-kappa B) contribute to the upregulation of Orai1 expression in PAH-hPASMCs. Using small interfering RNA (siRNA) and Orai1 inhibitors, we found that Orai1 inhibition reduced store-operated Ca2+ entry, mitochondrial Ca2+ uptake, aberrant proliferation, apoptosis resistance, migration, and excessive calcineurin activity in PAH-hPASMCs. Orai1 inhibitors reduced agonist-evoked constriction in human PAs. In experimental rat models of PH evoked by chronic hypoxia, monocrotaline, or Sugen/hypoxia, administration of Orai1 inhibitors (N-{4-[3,5-bis(Trifluoromethyl)-1H-pyrazol-1-yl]phenyl}-4-methyl-1,2,3-thiadiazole-5-carboxamide [BTP2], 4-(2,5-dimethoxyphenyl)-N-[(pyridin-4-yl)methyl]aniline [JPIII], or 5J4) protected against PH. Conclusions: In human PAH and experimental PH, Orai1 expression and activity are increased. Orai1 inhibition normalizes the PAH-hPASMCs phenotype and attenuates PH in rat models. These results suggest that Orai1 should be considered as a relevant therapeutic target for PAH.
Anti‐malarial drugs: Mechanisms underlying their proarrhythmic effects

Malaria remains the leading cause of parasitic death in the world. Artemisinin resistance is an emerging threat indicating an imminent need for novel combination therapy. Given the key role of mass drug administration, it is pivotal that the safety of anti‐malarial drugs is investigated thoroughly prior to widespread use. Cardiotoxicity, most prominently arrhythmic risk, has been a concern for anti‐malarial drugs. We clarify the likely underlying mechanisms by which anti‐malarial drugs predispose to arrhythmias. These relate to disruption of (1) action potential upstroke due to effects on the sodium currents, (2) action potential repolarisation due to effects on the potassium currents, (3) cellular calcium homeostasis, (4) mitochondrial function and reactive oxygen species production and (5) cardiac fibrosis. Together, these alterations promote arrhythmic triggers and substrates. Understanding these mechanisms is essential to assess the safety of these drugs, stratify patients based on arrhythmic risk and guide future anti‐malarial drug development.
Occupational violence and aggression in urgent and critical care in rural health service settings: A systematic review of mixed studies

Abstract Rural/remote health services are vulnerable to occupational violence and aggression due to factors such as weapon accessibility, poor network coverage and distance to backup. This systematic review investigated (1) the nature of occupational violence and aggression perpetrated in rural/remote health service urgent care settings and (2) the availability and effectiveness of policies/interventions/recommendations that address occupational violence and aggression in this context. We searched Business Source Complete, CINAHL Complete, Health & Society, APAIS Health, Health Collection, PsycINFO, PubMed, Scopus, SocIndex and Web of Science. Included articles (peer‐reviewed, no grey literature and English language) addressed occupational violence and aggression in rural health service urgent care settings. Fifteen articles matched these criteria (total [rural/remote only, where specified] N ~ 2555) and were included in the final analysis. The Mixed Methods Appraisal Tool was applied to assess the risk of bias. A data extraction table and narrative synthesis are presented. The most common occupational violence and aggression type was verbal aggression. The primary perpetrator was patients. Risk factors reflected practitioner age, remoteness, sector, staffing, shift type and area of practice. Precipitating factors were alcohol/drugs, dissatisfaction and mental health conditions. Policy content and limitations and education/training programme effectiveness were not addressed. Community collaboration supported occupational violence and aggression prevention/management. Organisational culture should promote reporting, debriefing and post‐incident care for staff well‐being. Work environment and job/task design are priorities for safety, but with possible limitations for traumatised clients. Occupational violence and aggression policies/interventions in rural health settings must be systematically evaluated to inform best practices. Co‐funded by Swinburne Social Innovation Research Institute Interdisciplinary Seed Funding Scheme and SMART Rural Health Network.
Criteria for assessing experiments in zero pressure gradient boundary layers

An extensive set of experimental data, for zero pressure gradient boundary layers, over a wide range of Reynolds number is re-evaluated with the help of a composite profile fitted to the mean-velocity data. Boundary layer parameters such as Π, H and the time scale ratio, Ω, are then carefully examined for consistency among the various experiments and their dependence on Reynolds number. Based on the predictions of the classical theory for these parameters, several criteria are established to evaluate whether a data set can be classified as ‘well-behaved’, i.e. representative of the desired ‘canonical’ state. We find that, when carefully applied, the different criteria are very consistent between themselves and can effectively be used interchangeably. The analysis can furthermore help identify the causes for deviations from the desired TBL state and hence serve as a guide in the design of future experiments.
MASP1 Gene Polymorphism and MASP-3 Serum Levels in Patients with Chronic Chagas Disease

ABSTRACT Introduction Chagas disease (CD), caused by Trypanosoma cruzi, is a major public health issue worldwide affecting 6–7 million people, mainly in Latin America. The complement system plays a crucial role in host immune defense against T. cruzi infection and during the chronic phase of CD; however, the role of the MBL-associated serine protease 1 (MASP1) gene encoding MASP-1, MASP-3, and MAp44 complement proteins has not yet been reported in CD. This study investigated the possible association between MASP1 gene polymorphisms and MASP-3 protein serum levels in chronic CD and its clinical forms. Methods Five polymorphisms of MASP1 gene regulatory regions were genotyped in 214 patients with CD and 197 healthy controls (rs7609662 G>A, rs13064994 C>T, rs72549262 C>G, rs1109452 C>T and rs850314 G>A). MASP-3 serum levels were assessed in 70 patients and 66 healthy controls. Clinical data, serum levels of complement proteins (ficolin-2, ficolin-3 and MBL) and inflammatory markers (pentraxin-3 and hsCRP) were also included in the analyses. Results A significant association of the MASP1 GC_CCA haplotype with CD (padj= 0.002; OR 3.17 [1.19–8.39]) and chronic chagasic cardiomyopathy (CCC) (padj= 0.013; OR 4.57 [1.37–15.16] was observed. MASP-3 and pentraxin-3 levels were positively correlated in the patients (rho = 0.62; p = 0.0001). MASP-3 levels were not associated with MASP1 polymorphisms or CD and its clinical forms. Furthermore, no correlation was observed between MASP-3 levels and that of ficolin-2, ficolin-3, MBL and hsCRP. Conclusion Our findings suggest a possible role for the MASP1 GC_CCA haplotype in susceptibility to chronic CD and CCC clinical forms. GRAPHICAL ABSTRACT
Validity of Gymnastics-Specific Assessment of Neuromuscular Function of Shoulder Flexor and Extensor Muscles to Predict Performance in Gymnastics Skills

Abstract Milosis, DC. Validity of gymnastics-specific assessment of neuromuscular function of shoulder flexor and extensor muscles to predict performance in gymnastics skills. J Strength Cond Res 37(3): 652–660, 2023—This study examined the relationships between gymnastics-specific laboratory measurements of neuromuscular function of the shoulder flexor and extensor muscles and performance in specific gymnastics skills. Fifteen highly competitive male gymnasts and 18 male students (age 20.41 ± 2.51 years, body mass 69.7 ± 7.07 kg; mean ± SD) participated voluntarily. The electromyogram (EMG) was used to assess muscle activation of 3 flexor and 3 extensor shoulder muscles during isometric (45, 90, and 135°) and isokinetic (concentric and eccentric 60, 180, and 300°/s) flexion-extension of the shoulder. Peak torque (PT), neuromuscular efficiency (NME: torque to EMG ratio), and antagonist coactivation index (CI) were evaluated. Furthermore, performance in specific gymnastics skills was evaluated. The results supported the ecological validity of the measurements. Specifically, significant correlations emerged between the shoulders' neuromuscular function parameters and the performance in the gymnastics skills (PT: r = 0.798, p < 0.001, NME: r = 0.576, p < 0.001, CI: r = −0.351, p < 0.05). In addition, according to the results of the regression analysis, neuromuscular function of the shoulder flexor and extensor muscles predicted gymnastics performance. Specifically, the model that was produced significantly predicted the dependent variable (performance in specific gymnastics skills) in all steps and explained a total variance of 64.4% (p < 0.001). Based on beta coefficients, PT had the higher contribution to the prediction of performance. The insight gained on these relationships could help coaches to plan the training of their gymnasts and to monitor the longitudinal changes in neuromuscular function of shoulder muscles induced by training more effectively.
PtdIns(3,4)P2, Lamellipodin, and VASP coordinate actin dynamics during phagocytosis in macrophages

Class I PI 3-kinase is required for phagocytosis. This study investigates the role of PI3,4P2 produced downstream of PI 3-Kinase in phagocytosis. PI3,4P2, its effector-binding protein Lamellipodin, and VASP are required to support actin-driven pseudopod extension and engulfment of IgG opsonized particles.
Classification of ECG signal using FFT based improved Alexnet classifier

Electrocardiograms (ECG) are extensively used for the diagnosis of cardiac arrhythmias. This paper investigates the use of machine learning classification algorithms for ECG analysis and arrhythmia detection. This is a crucial component of a conventional electronic health system, and it frequently necessitates ECG signal reduction for long-term data storage and remote transmission. Signal processing methods must be used to extract the function of the morphological properties of the ECG signal changing with time, which is difficult to discern in the typical visual depiction of the ECG signal. In biomedical research, signal processing and data analysis are commonly employed methodologies. This work proposes the use of an ECG arrhythmia classification method based on Fast Fourier Transform (FFT) for feature extraction and an improved AlexNet classifier to distinguish the difference between four types of arrhythmia conditions that were collected from records. The Convolutional Neural Network (CNN) algorithm’s results are compared to those of other algorithms, and the simulation results prove that the proposed technique is more effective for various parameters. The final results of the proposed system show that its ability to find deviations is 20% better than that of traditional systems.
Pressure, density, temperature and entropy fluctuations in compressible turbulent plane channel flow

Abstract We investigate the fluctuations of thermodynamic state variables in compressible aerodynamic wall turbulence, using results of direct numerical simulation (DNS) of compressible turbulent plane channel flow. The basic transport equations governing the behaviour of thermodynamic variables (density, pressure, temperature and entropy) are reviewed and used to derive the exact transport equations for the variances and fluxes (transport by the fluctuating velocity field) of the thermodynamic fluctuations. The scaling with Reynolds and Mach number of compressible turbulent plane channel flow is discussed. Statistics and correlation coefficients of the thermodynamic fluctuations are examined. Finally, detailed budgets of the transport equations for the variances and fluxes of the thermodynamic variables are analysed. The implications of these results, leading both to the understanding of the thermodynamic interactions in compressible wall turbulence and to possible improvements in statistical modelling, are assessed. Finally, the required extension of existing DNS data to fully characterise this canonical flow is discussed.
Jump Height and Hip Power Decrease During Cognitive Loading Regardless of Sex: Implications for Sport Performance Metrics

Abstract Shumski, EJ, Lempke, LB, Johnson, RS, Oh, J, Schmidt, JD, and Lynall, RC. Jump height and hip power decrease during cognitive loading regardless of sex: implications for sport performance metrics. J Strength Cond Res 37(4): 793–798, 2023—Sex and cognitive loading separately influence jumping performance. However, it is unknown how cognitive loading influences jump performance and how sex and cognitive loading interact. The purpose of our study was to determine if an interaction existed between sex and cognitive loading for the dependent variables jump height, ground contact time, reactive strength index, vertical stiffness, impulse, and lower extremity joint power during a double limb drop vertical jump. Twenty-one male (23.2 ± 2.5 years, 180.8 ± 8.4 cm, 80.4 ± 10.2 kg) and 20 female (21.7 ± 1.0 years, 163.7 ± 8.2 cm, 61.2 ± 9.4 kg) physically active individuals participated. Subjects jumped from a 30 cm box placed 50% of their height away from 2 force plates under single-task and dual-task (serial 6s or 7s) conditions. Separate 2 × 2 analyses of variance were used for all dependent variables (α = 0.05) with Bonferroni post hoc mean differences and 95% confidence intervals (CIs). There were no significant interactions for any outcomes (p ≥ 0.190). Condition main effects demonstrated subjects jumped significantly higher (1.84 cm, 95% CI = 0.68–3.01, d = 0.26, p = 0.003) and with greater hip power (0.29 Watts·BW−1·HT−1, 95% CI = 0.04–0.54, d = 0.21, p = 0.025) during single task compared with dual task. Sex main effects revealed males jumped higher (9.88 cm, 95% CI = 7.00–12.77, d = 2.17, p < 0.001), with greater reactive strength index (0.29, 95% CI = 0.17–0.41, d = 1.52, p < 0.001), greater ankle power (3.70 Watts·BW−1·HT−1, 95% CI = 2.26–5.13, d = 1.64, p < 0.001), and greater knee power (5.00 Watts·BW−1·HT−1, 95% CI = 3.25–6.75, d = 1.82, p < 0.001) compared with females. Jump performance is influenced by sex and dual-task conditions but not their interaction. To optimize jumping performance, testing should be completed without distractions (single task) to decrease cognitive loading.