Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of memory T cell subsets and common γ−chain cytokines in convalescent COVID‐19 individuals

T cells are thought to be an important correlates of protection against SARS‐CoV2 infection. However, the composition of T cell subsets in convalescent individuals of SARS‐CoV2 infection has not been well studied. The authors determined the lymphocyte absolute counts, the frequency of memory T cell subsets, and the plasma levels of common γ−chain in 7 groups of COVID‐19 individuals, based on days since RT‐PCR confirmation of SARS‐CoV‐2 infection. The data show that both absolute counts and frequencies of lymphocytes as well as, the frequencies of CD4+ central and effector memory cells increased, and the frequencies of CD4+ naïve T cells, transitional memory, stem cell memory T cells, and regulatory cells decreased from Days 15–30 to Days 61–90 and plateaued thereafter. In addition, the frequencies of CD8+ central memory, effector, and terminal effector memory T cells increased, and the frequencies of CD8+ naïve cells, transitional memory, and stem cell memory T cells decreased from Days 15–30 to Days 61–90 and plateaued thereafter. The plasma levels of IL‐2, IL‐7, IL‐15, and IL‐21—common γc cytokines started decreasing from Days 15–30 till Days 151–180. Severe COVID‐19 patients exhibit decreased levels of lymphocyte counts and frequencies, higher frequencies of naïve cells, regulatory T cells, lower frequencies of central memory, effector memory, and stem cell memory, and elevated plasma levels of IL‐2, IL‐7, IL‐15, and IL‐21. Finally, there was a significant correlation between memory T cell subsets and common γc cytokines. Thus, the study provides evidence of alterations in lymphocyte counts, memory T cell subset frequencies, and common γ−chain cytokines in convalescent COVID‐19 individuals.
Influence of dietary lycopene on growth performance, antioxidant status, blood parameters and mortality in broiler chicken with cold-induced ascites

ABSTRACT The aim of this study was to determine the effects of dietary lycopene supplementation on growth performance and antioxidant status of broiler chickens exposed to cold environment to induce ascites. Three hundred male chickens were exposed full-day to cold stress (CT, 10°C) starting from day 15 of age until the end of experiment at day 42, while a positive control group (NT, 100 birds) was kept under normal temperature (23–25°C). The CT groups (three treatments and five replicates of 20 birds) were as follows: negative control (basal diet, CT) and CT + 200 or 400 mg lycopene per kg diet from 15 to 42 d of age. Results showed that CT without lycopene supplementation caused a reduction of feed intake and weight gain and increased the feed conversion ratio. Supplementation of lycopene during CT restored the performance to levels of the positive control, lowered the index of right ventricles/total ventricles and ascites mortality. Birds reared under CT had lower serum activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and higher malondialdehyde (MDA) concentration than birds reared under the NT environment. With lycopene supplementation, serum MDA level significantly decreased and the activity of SOD and GPx increased. Blood concentration of haematocrit, haemoglobin and red blood cells were decreased by the highest lycopene supplementation to a level comparable to NT. Moreover, increasing dietary lycopene level suppressed serum concentrations of cholesterol and enhanced high-density lipoproteins levels in blood. In conclusion, lycopene supplementation alleviates adverse effects of cold stress on performance through modulating activity of antioxidant enzymes in broiler chickens.
Translational Pharmacology of PRAX‐944, a Novel T‐Type Calcium Channel Blocker in Development for the Treatment of Essential Tremor

Essential tremor is the most common movement disorder with clear unmet need. Mounting evidence indicates tremor is caused by increased neuronal burst firing and oscillations in cerebello‐thalamo‐cortical circuitry and may be dependent on T‐type calcium channel activity. T‐type calcium channels regulate sigma band electroencephalogram (EEG) power during non‐rapid eye movement sleep, representing a potential biomarker of channel activity. PRAX‐944 is a novel T‐type calcium channel blocker in development for essential tremor.
Progress and limitations of oral peptide delivery as a potentially transformative therapy

ABSTRACT Introduction Numerous formulation technologies have been developed to overcome challenges of oral peptide delivery. Understanding the advantages and limitations of each technology is important for the development of new delivery systems to enable oral delivery of peptides designed for parenteral administration. Areas covered This review summarizes key learnings from the use of permeation enhancers (PEs) for oral peptide delivery associated with solid dosage form optimization to maximize the PE effect. Furthermore, we will highlight the most recent emerging delivery strategies to improve oral peptide bioavailability such as nanoparticles, self-emulsifying drug delivery systems, gut shuttles, and ingestible devices. In addition, advantages and limitations of these technologies will be compared with the permeation enhancer technology. Expert opinion Despite the success of permeation enhancer technology in the FDA-approved oral peptide products, oral peptide delivery is still facing the immense challenge of low-to-single digit oral bioavailability. Optimization of drug product attributes such as dissolution kinetics is critical to improve permeation enhancer efficacy. The next frontiers to substantially increase oral bioavailability and transform injectable peptides to oral deliverables may be ingestible devices and ligand-mediated transport (gut shuttles). However, clinical studies are necessary to inform the safety and efficacy of these emerging technologies.
Preference and ability to perform blood-versus oral-fluid-based HIV self-testing in adolescents and young adults in Bangkok

Introduction: Adolescents and young adults (AYA) have limited access to HIV screening tests despite the risk of acquiring HIV infection. This study aims to understand AYA preferences and their ability to perform HIV self-tests (HIVST). Methods: A cross-sectional study looked at AYA preferences when offered a choice between blood-based (INSTI®) and oral fluid-based (OraQuick®) HIVST. Adolescents and young adult participants between 18 and 24 years-old who report inconsistent condom use or had a history of sexually transmitted diseases were enrolled. Participants were offered a choice between blood-based and/or oral fluid-based HIVSTs with explanations of the differences between two types. Then, written and short video instructions according to the chosen type were given before participants performed a test. The study seeks to understand test preference, ability to perform and interpret test results. Results: From March to April 2021, 87 AYA were enrolled with a median age of 20 years (interquartile range (IQR) 18–22). Of the participants, 54 (62.1%) were men who have sex with men (MSM), 25 (28.7%) were cisgender men or women and 8 (9.2%) were transgender women (TGW). There were 37 (42.5%) first-time HIV testers and 32 (36.8%) HIV PrEP users. There were 57 participants (65.6%, 95% CI 54.6%–75.4%) that preferred blood-based HIVSTs. Reasons for preferring blood-based testing were the rapid results (77.2%) and higher accuracy (66.7%). The ability to perform and interpret HIVST results were 89.5% and 98% among INSTI users and 93.3% and 100.0% among OraQuick® users. None was HIV-positive. Moreover, 13.8% of the participants initiated same-day pre-exposure prophylaxis (PrEP). Conclusions: Thai AYA preferred blood-based over oral fluid-based HIVSTs. Most AYAs were able to perform the HIVSTs and interpret results. Supervision and post-test counselling of HIVSTs should be implemented to ensure AYA gain benefits from HIVSTs.
Half of US population exposed to adverse lead levels in early childhood

Significance Considerable effort is expended to protect today’s children from lead exposure, but there is little evidence on the harms past lead exposures continue to hold for yesterday’s children, who are victims of what we term legacy lead exposures. We estimate that over 170 million Americans alive today were exposed to high-lead levels in early childhood, several million of whom were exposed to five-plus times the current reference level. Our estimates allow future work to plan for the health needs of these Americans and to inform estimation of the true contributions of lead exposure to population health. We estimate population-level effects on IQ loss and find that lead is responsible for the loss of 824,097,690 IQ points as of 2015.
3D Image Registration Marginally Improves the Precision of HR‐pQCT Measurements Compared to Cross‐Sectional‐Area Registration in Adults With Osteogenesis Imperfecta

Repositioning error in longitudinal high‐resolution peripheral‐quantitative computed tomography (HR‐pQCT) imaging can lead to different bone volumes being assessed over time. To identify the same bone volumes at each time point, image registration is used. While cross‐sectional area image registration corrects axial misalignment, 3D registration additionally corrects rotations. Other registration methods involving matched angle analysis (MA) or boundary transformations (3D‐TB) can be used to limit interpolation error in 3D‐registering micro‐finite‐element data. We investigated the effect of different image registration methods on short‐term in vivo precision in adults with osteogenesis imperfecta, a collagen‐related genetic disorder resulting in low bone mass, impaired quality, and increased fragility. The radii and tibiae of 29 participants were imaged twice on the same day with full repositioning. We compared the precision error of different image registration methods for density, microstructural, and micro‐finite‐element outcomes with data stratified based on anatomical site, motion status, and scanner generation. Regardless of the stratification, we found that image registration improved precision for total and trabecular bone mineral densities, trabecular and cortical bone mineral contents, area measurements, trabecular bone volume fraction, separation, and heterogeneity, as well as cortical thickness and perimeter. 3D registration marginally outperformed cross‐sectional area registration for some outcomes, such as trabecular bone volume fraction and separation. Similarly, precision of micro‐finite‐element outcomes was improved after image registration, with 3D‐TB and MA methods providing greatest improvements. Our regression model confirmed the beneficial effect of image registration on HR‐pQCT precision errors, whereas motion had a detrimental effect on precision even after image registration. Collectively, our results indicate that 3D registration is recommended for longitudinal HR‐pQCT imaging in adults with osteogenesis imperfecta. Since our precision errors are similar to those of healthy adults, these results can likely be extended to other populations, although future studies are needed to confirm this. © 2022 American Society for Bone and Mineral Research (ASBMR).
Patterns of Traumatic Spinal Injuries in the Developing World: A Five-Year Longitudinal Review

Study Design Retrospective review. Purpose A 5-year longitudinal study documenting and comparing patterns of traumatic spinal injuries (TSIs) observed in developing countries. Overview of Literature Current knowledge of the patterns and epidemiology of TSI are based on evidence from developed countries and there is a lack of data from developing countries to enable a comparison of information to formulate healthcare policies. Methods A review of case records of all patients treated at a tertiary level trauma center over a 5-year period (2015–2019) was performed. Epidemiological, clinical, and radiological data were analyzed. Results The incidence of spinal trauma was 6.2% (2,065/33,072) among all trauma patients. Among these 2,065 patients, the mean age was 43.4±16.3 years and 77.3% (n=1,596) were aged 21–60 years. The major cause of injury was falls (52.1%, n=1,069) and 49.8% were high-energy falls (>10 feet [=3.048 m]). In patients with TSI due to falls, injuries occurred at the workplace (n=376), home (n=309), trees (n=151), wells (n=77), and electric poles (n=57). Road traffic accidents contributed to 42% (n=862) of TSIs and predominantly affected motorcyclists (52%, n=467). Around half (53.5%, n=1,005) of all patients were in the lower socioeconomic strata. The most common injury level was thoracic region (37.2%, n=769). Spinal cord injury (SCI) occurred in 49% (n=1,011) of patients and 49.7% (n=1,028) had injuries associated with other organs. Conclusions Our study indicated different demographic patterns and epidemiological features of TSI compared with the Western literature, including a preponderance of young male patients, falls from heights, motorcycle accidents, and a larger percentage of SCI. The high number of falls at workplace indicates a lack of knowledge among the public and policy makers about safety measures.
Using Heart Rate Variability for Comparing the Effectiveness of Virtual vs Real Training Environments for Firefighters

The use of Virtual Reality (VR) technology to train professionals has increased over the years due to its advantages over traditional training. This paper presents a study comparing the effectiveness of a Virtual Environment (VE) and a Real Environment (RE) designed to train firefighters. To measure the effectiveness of the environments, a new method based on participants’ Heart Rate Variability (HRV) was used. This method was complemented with self-reports, in the form of questionnaires, of fatigue, stress, sense of presence, and cybersickness. An additional questionnaire was used to measure and compare knowledge transfer enabled by the environments. The results from HRV analysis indicated that participants were under physiological stress in both environments, albeit with less intensity on the VE. Regarding reported fatigue and stress, the results showed that none of the environments increased such variables. The results of knowledge transfer showed that the VE obtained a significant increase while the RE obtained a positive but non-significant increase (median values, VE: before – 4 after – 7, p = .003; RE: before – 4 after – 5, p = .375). Lastly, the results of presence and cybersickness suggested that participants experienced high overall presence and no cybersickness. Considering all results, the authors conclude that the VE provided effective training but that its effectiveness was lower than that of the RE.
COVID-19 intranasal vaccines: current progress, advantages, prospects, and challenges

ABSTRACT Multiple vaccines have recently been developed, and almost all the countries are presently vaccinating their population to tackle the COVID-19 pandemic. Most of the COVID-19 vaccines in use are administered via intramuscular (IM) injection, eliciting protective humor and cellular immunity. COVID-19 intranasal (IN) vaccines are also being developed that have shown promising ability to induce a significant amount of antibody-mediated immune response and a robust cell-mediated immunity as well as hold the added ability to stimulate protective mucosal immunity along with the additional advantage of the ease of administration as compared to IM injected vaccines. By inducing secretory IgA antibody responses specifically in the nasal compartment, the intranasal SARS-CoV-2 vaccine can prevent virus infection, replication, shedding, and disease development, as well as possibly limits virus transmission. This article highlights the current progress, advantages, prospects, and challenges in developing intranasal COVID-19 vaccines for countering the ongoing pandemic.
Educational technologies as matters of care

ABSTRACT Schools, teaching, and learning environments have long been understood and idealised as places of care. Within feminist studies and Science and Technology Studies (STS), scholars have proposed a shift to include non-humans in what we propose to conceive as care arrangements. Drawing on Tronto's feminist ethics of care [1993. Moral Boundaries: A Political Argument for an Ethic of Care. Routledge], we investigate educational technologies as ‘matters of care’, facilitating and enabling different modes of care. We pay particular attention to the role that educational technologies assume in schools’ care arrangements. The paper is based on a study of four school information systems in Germany. We identify how these systems can be conceived of as antagonists, intermediaries, recipients of care or means to receive care and critically reflect on how this allows us to imagine modes of care with technology in order to create good educational futures.
A study on plastic strain accumulation caused by traveling of temperature distribution synchronizing with temperature rise

The prevention of excessive deformation by thermal ratcheting is important in the design of high-temperature components of fast breeder reactors (FBR). This includes evaluation methods for a new type of thermal ratcheting caused by an axial traveling of temperature distribution, which corresponds to moving-up of liquid sodium surface in startup phase. Long range traveling of the axial temperature distribution brings flat plastic deformation profile in wide range. Therefore, at the center of this range, residual stress that brings shakedown behavior does not accumulate. As a result, repeating of this temperature traveling brings continuous accumulation of the plastic strain, even if there is no primary stress. In contrast, in the case with short range traveling, residual stress is caused by constraint against elastic part, and finally it results in shakedown. Because of this mechanism, we supposed that limit for the shakedown behavior depends on distance from the elastic part (i.e. half length of region with plastic deformation). In this paper, we examined characteristics of the accumulation of the plastic strain caused by realistic heat transients, namely, traveling of temperature distribution synchronizing with temperature rise. This examination was based on finite element analyses using elastic-perfectly plastic material. As a result, we confirmed that the shakedown limit depends not on the traveling range of the temperature distribution but the plastic deformation range, which was predicted by the elastic analysis. In the actual application, we can control the plastic deformation range by changing rate of the moving-up of liquid sodium surface.
Stochastic rounding: implementation, error analysis and applications

Stochastic rounding (SR) randomly maps a real number x to one of the two nearest values in a finite precision number system. The probability of choosing either of these two numbers is 1 minus their relative distance to x. This rounding mode was first proposed for use in computer arithmetic in the 1950s and it is currently experiencing a resurgence of interest. If used to compute the inner product of two vectors of length n in floating-point arithmetic, it yields an error bound with constant nu with high probability, where u is the unit round-off. This is not necessarily the case for round to nearest (RN), for which the worst-case error bound has constant nu. A particular attraction of SR is that, unlike RN, it is immune to the phenomenon of stagnation, whereby a sequence of tiny updates to a relatively large quantity is lost. We survey SR by discussing its mathematical properties and probabilistic error analysis, its implementation, and its use in applications, with a focus on machine learning and the numerical solution of differential equations.
Divergent paths in the evolutionary history of maternally transmitted clam symbionts

Vertical transmission of bacterial endosymbionts is accompanied by virtually irreversible gene loss that results in a progressive reduction in genome size. While the evolutionary processes of genome reduction have been well described in some terrestrial symbioses, they are less understood in marine systems where vertical transmission is rarely observed. The association between deep-sea vesicomyid clams and chemosynthetic Gammaproteobacteria is one example of maternally inherited symbioses in the ocean. Here, we assessed the contributions of drift, recombination and selection to genome evolution in two extant vesicomyid symbiont clades by comparing 15 representative symbiont genomes (1.017–1.586 Mb) to those of closely related bacteria and the hosts' mitochondria. Our analyses suggest that drift is a significant force driving genome evolution in vesicomyid symbionts, though selection and interspecific recombination appear to be critical for maintaining symbiont functional integrity and creating divergent patterns of gene conservation. Notably, the two symbiont clades possess putative functional differences in sulfide physiology, anaerobic respiration and dependency on environmental vitamin B12, which probably reflect adaptations to different ecological habitats available to each symbiont group. Overall, these results contribute to our understanding of the eco-evolutionary processes shaping reductive genome evolution in vertically transmitted symbioses.
Highly magmatic break-up LIP centres: revisiting the East Greenland volcanic rifted margin

Abstract The decompressional release of magma at continental triple-rift break-up, large igneous province (LIP) centres, above mantle plume stems, results in highly magmatic settings. As a particularly well-exposed example, it is proposed that the East Greenland coastal dyke swarm preserves a structural record of how dyke dilations v. tectonic extension increased upon approaching its Kangerlussuaq triple rift centre. Such more magmatic break-up is reflected by how abruptly its volcanic rifted margin transitions into 100% dykes, and, in this case, up to 100 km further inland than its geophysically determined continent–ocean boundary. Correspondingly high magma flux through an igneous Kap Edward Holm centre sustained the lateral injection of up to 150 km-long dykes, evidenced by increased cut-off dyke thicknesses, below which there is an anomalously low abundance of thinner dykes, that conform to the cube root of their thermal arrest distance. Only the thickest and, thereby, longest dyke injections linked up with a more southerly located igneous Imilik centre of an en echelon offset dyke swarm, the complex transition into which is also addressed. This highly magmatic central plumbing system is further compared to similar volcanic zones across Iceland and other post-Paleozoic break-up LIPs in order to contextualize its importance.
Ground Temperature Variability in Poznań (2011–2020)

Abstract The manuscript concerns the analysis of the ground temperature in Poznań in the years 2011–2020. Data generally available on the Institute of Meteorology and Water Management – National Research Institute (IMGW-PIB) website were used. The ground temperature was measured at five depths: 5 cm, 10 cm, 20 cm, 50 cm and 100 cm. The highest variability of ground temperature occurred in summer and the lowest in winter. The ground temperature was closely correlated mainly with air temperature, but also with insolation and water vapour deficit. There is a statistical increase in the air temperature in Poznań – the trends at the whole soil profile are positive although not statistically significant.