Miracle-dz/UniFAR-FTUs huggingface dataset:
Long-Term Dominance of Carbapenem-Non-Susceptible Pseudomonas aeruginosa ST111 in Hematologic Malignancy Patients and Hematopoietic Cell Transplant Recipients

An epidemiological study uncovered that fluoroquinolone (FQ) neutropenic prophylaxis in hematopoietic cell transplant and hematologic malignancy (HCT/HM) patients was associated with breakthrough Pseudomonas aeruginosa bloodstream infections (BSIs) with isolates non-susceptible to both FQs and meropenem. The molecular epidemiology of the FQ/meropenem-non-susceptible P. aeruginosa isolates causing FQ-breakthrough BSIs in the HCT/HM patients remains unclear. Through whole genome sequencing on 57 P. aeruginosa isolates from 54 patients diagnosed with HM or receiving an HCT, we found that ST111 strains predominated, accounting for 22 (38.6%) of the isolates. 17 of 33 (51.5%) FQ-breakthrough BSIs were caused by ST111 strains, of which 15 (88.2%) were meropenem non-susceptible. ST111 strains, but not other oprD-deficient, meropenem-non-susceptible clinical strains, were found to have a colonization advantage over P. aeruginosa strain PA14 in C. elegans and to outcompete PA14 in in vitro co-culture assays. Together, we found that breakthrough P. aeruginosa BSIs during FQ prophylaxis in HCT/HM patients are dominated by clonally-related FQ/meropenem non-susceptible strains, predominantly ST111 type, and that the dominance of ST111 strains may be explained by a relative fitness advantage over other clinical strains. Additional work is necessary to better understand the factors driving the dominance and persistence of these ST111 strains.
E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model

For patients with chronic limb-threatening ischemia and limited revascularization options, alternate means for therapeutic angiogenesis and limb salvage are needed. E-selectin is a cell adhesion molecule that is critical for inflammation and neovascularization in areas of wound healing and ischemia. Here, we tested the efficacy of modifying ischemic limb tissue by intramuscular administration of E-selectin/AAV2/2 (adeno-associated virus serotype 2/2) to modulate angiogenic and inflammatory responses in a murine hindlimb gangrene model. Limb appearance, reperfusion, and functional recovery were assessed for 3 weeks after induction of ischemia. Mice receiving E-selectin/AAV2/2 gene therapy had reduced gangrene severity, increased limb and footpad perfusion, enhanced recruitment of endothelial progenitor cells, and improved performance on treadmill testing compared to control group. Histologically, E-selectin/AAV2/2 gene therapy was associated with increased vascularity and preserved myofiber integrity. E-selectin/AAV2/2 gene therapy also upregulated a panel of pro-angiogenic genes yet downregulated another group of genes associated with the inflammatory response. This novel gene therapy did not induce adverse effects on coagulability, or hematologic, hepatic, and renal function. Our findings highlight the potential of E-selectin/AAV2/2 gene therapy for improving limb perfusion and function in patients with chronic limb-threatening ischemia.
A New Variant of Mutational and Polymorphic Signatures in the ERG11 Gene of Fluconazole-Resistant Candida albicans

Background Resistance to antifungal drugs for treating Candida infections remains a major concern globally despite the range of medications available. Most of these drugs target key proteins essential to the life cycle of the organism. An enzyme essential for fungal cell membrane integrity, lanosterol 14–α demethylase (CYP51), is encoded by the ERG11 gene in Candida species. This enzyme is the target of azole–based drugs. The organism has, however, devised molecular adaptations to evade the activity of these drugs. Materials and Methods Classical methods were employed to characterize clinical isolates sampled from women and dogs of reproductive age. For fluconazole efficacy studies, CLSI guidelines on drug susceptibility testing were used. To understand the susceptibility pattern, various molecular and structural analytic approaches, including sequencing, in silico site-directed mutagenesis, and protein-ligand profiling, were applied to the ERG11 gene and CYP51 protein sequences. Several platforms, comprising Clustal Omega, Pymol plugin manager, Pymol molecular visualizer, Chimera–curated Dynameomics rotamer library, protein–ligand interaction profiler, Charmm36 force field, GROMACS, Geneious, and Mega7, were employed for this analysis. Results The following Candida species distribution was obtained: 37.84% C. albicans, 8.12% C. glabrata, 10.81% C. krusei, 5.41% C. tropicalis, and 37.84% of other unidentified Candida species. Two codons in the nucleotide sequence of the wild-type (CTC and CCA) coding for LEU–370 and PRO–375, respectively, were mutated to L370S and P375H in the resistant strain. The mutation stabilized the protein at the expense of the heme moiety. We found that the susceptible isolate from dogs (Can–iso–029/dog) is closely related to the most resistant isolate from humans. Conclusion Taken together, our results showed new mutations in the heme-binding pocket of caCYP51 that explain the resistance to fluconazole exhibited by the Candida isolates. So far, the L370S and P375H resistance-linked mutations have not been previously reported.
Friction Force Adjustment by an Innovative Covering System Applied with Superelastic NiTi Brackets and Wires—An In-Vitro Study

The aim of this study was the investigation of polymeric coverings to adjust frictional forces between V-shaped wires and brackets, both made of superelastic NiTi. Adjustment of frictional forces is relevant for certain stages during orthodontic therapy. Coverings able to generate frictional forces when assembled to such brackets are additively manufactured. Six different internal widths of coverings were examined in three different environments: dry condition at room temperature (RT) or body temperature (BT), or artificial saliva (AS) at RT. The different coverings significantly affected the frictional forces for all media (p < 0.001). A correlation between internal width of the covering and resulting frictional forces was found. BT and dry environment showed the lowest friction forces for all samples. The highest force was found for two covering types at RT in AS, while the remaining four covering types showed the highest values in dry environment (p < 0.001). Friction could, therefore, be adjusted by variation of bracket covering clipped onto brackets, which is useful for orthodontic therapy. Coverings delivering higher friction provide dental anchorage, while coverings with lower friction can be used for tooth movement or purely esthetic reasons. It was shown that the variation of covering width may be used for adjustment of frictional forces.
Antioxidative, Anti-Inflammatory, Anti-Obesogenic, and Antidiabetic Properties of Tea Polyphenols—The Positive Impact of Regular Tea Consumption as an Element of Prophylaxis and Pharmacotherapy Support in Endometrial Cancer

Endometrial cancer (EC) is second only to cervical carcinoma among the most commonly diagnosed malignant tumours of the female reproductive system. The available literature provides evidence for the involvement of 32 genes in the hereditary incidence of EC. The physiological markers of EC and coexisting diet-dependent maladies include antioxidative system disorders but also progressing inflammation; hence, the main forms of prophylaxis and pharmacotherapy ought to include a diet rich in substances aiding the organism’s response to this type of disorder, with a particular focus on ones suitable for lifelong consumption. Tea polyphenols satisfy those requirements due to their proven antioxidative, anti-inflammatory, anti-obesogenic, and antidiabetic properties. Practitioners ought to consider promoting tea consumption among individuals genetically predisposed for EC, particularly given its low cost, accessibility, confirmed health benefits, and above all, suitability for long-term consumption regardless of the patient’s age. The aim of this paper is to analyse the potential usability of tea as an element of prophylaxis and pharmacotherapy support in EC patients. The analysis is based on information available from worldwide literature published in the last 15 years.
The Role of Bile Acid in Improving Glucose Tolerance of Non-Obese Diabetic Rats After Proximal Small Bowel Bypass

An increase in bile acid (BA) levels after metabolic surgery is an important mechanism for improving glucose metabolism. However, the mechanisms underlying elevated BA levels and the regulatory mechanism of glucose metabolism remain unclear. In this study, we used the Goto-Kakizaki rat model to investigate the mechanism of BA elevation by comparing side-to-side jejunoileal bypass plus proximal loop ligation (SSJIBL) and bile ileum diversion (BID) as well as to explore the mechanism of BA metabolism in regulating blood glucose. The results showed that the fed blood glucose of rats in both the SSJIBL and BID groups was significantly lower than that of the SHAM group on days 2 and 14 after the operation. The oral glucose tolerance test (OGTT) improved in the SSJIBL and BID groups at day 14 postoperatively. The expression of CYP27A1 in the livers of the SSJIBL and BID groups was significantly increased. In addition, total serum BA levels in the SSJIBL and BID groups were significantly increased. Moreover, serum levels of lithocholic acid (LCA) and deoxycholic acid (DCA) were significantly higher in the SSJIBL group than in the SHAM group and negatively correlated with the area under the glucose tolerance curve (AUC-OGTT). In conclusion, increased BA synthesis may be an important cause of elevated total serum BA levels, and LCA and DCA are closely associated with improved glucose metabolism.
TRAINER PID CONTROLLER SEBAGAI MEDIA PEMBELAJARAN PRAKTIK SISTEM KENDALI

Penelitian ini bertujuan untuk mengembangkan trainer PID controller dan menilai kelayakan trainer PID Controller. Trainer yang dikembangkan menggunakan aplikasi PID controller yaitu pengendali dual brushless . Penelitian ini merupakan penelitian R&D yang berorientasi produk berupa trainer dan modul pendamping. Setelah produk mendapat validasi dari ahli melalui uji alfa dan uji beta maka produk dievaluasi pada implementasi sesungguhnya. Pengembangan media merujuk pada model Lee & Owens. Penelitian ini menggunakan model pengembangan yang meliputi analisis, desain, implementasi dan evaluasi, dimana letak evaluasi dan revisi terletak pada tiap tahapan. Evaluasi dilakukan oleh ahli materi, ahli media dan pengguna. Pengambilan data menggunakan angket yang memiliki rentang nilai 1 s.d. 4. Perolehan nilai angket selanjutnya dikonversi untuk mengetahui kategori kelayakan. Hasil penelitian menunjukkan nilai kelayakan trainer PID Controller adalah 3,33 dan termasuk kategori Sangat Baik. Karena trainer PID Controller memperoleh nilai kelayakan sangat baik maka trainer ini dapat digunakan dalam pembelajaran di kelas
Mechanisms of bicarbonate secretion in the equine colon ex vivo.

OBJECTIVE To examine bicarbonate (HCO3-) secretion ex vivo in the equine large colon to determine any differences between the right dorsal colon (RDC) and right ventral colon (RVC). The effect of phenylbutazone (PBZ) on HCO3- secretion was examined in the RDC. ANIMALS 14 healthy horses. PROCEDURES In anesthetized horses (n = 10), segments of mucosa from RDC and RVC were harvested to measure HCO3- secretion ex vivo with the pH Stat method. The effect of PBZ on HCO3- secretion in the RDC was studied in 4 additional horses. RESULTS Three distinct mechanisms of HCO3- secretion previously described in a murine model were confirmed in the equine colon. The RDC had a greater capacity for electrogenic, Cl--independent HCO3- secretion than the RVC (P = 0.04). In the RDC, all HCO3- secretion was decreased by PBZ (P < 0.02) but was not studied in the RVC because of low baseline secretion. CLINICAL RELEVANCE Secretion of HCO3- by the RDC could play a pivotal role in equine colon physiology, because intense microbial fermentation in this site could require HCO3- secretion to buffer short-chain fatty acids. Inhibition of this secretion by PBZ could interfere with mucosal buffering and predispose to changes associated with right dorsal colitis.
Multi-Faceted Influence of Obesity on Type 1 Diabetes in Children – From Disease Pathogenesis to Complications

The prevalence of overweight and obesity among youth patients with diabetes type 1 is increasing. It is estimated, that even up to 35% of young patients with this type of diabetes, considered so far to be characteristic for slim figure, are overweight or even obese. General increase of obesity in children’s population complicates differential diagnosis of the type of diabetes in youths. Coexistence of obesity has clinical implications for all stages of diabetes course. It is confirmed that obesity is the risk factor for autoimmune diabetes, and is connected with the earlier onset of diabetes in predisposed patients. Many diabetic patients with obesity present additional risk factors for macroangiopathy, and are recognised to present metabolic syndrome, insulin resistance, and typical for diabetes type 2 - polycystic ovary syndrome, or non-alcoholic fatty liver disease. The prevalence of obesity rises dramatically in adolescence of diabetic child, more often in girls. It has negative impact on metabolic control, glycaemic variability and insulin demand. The risk for microangiopathic complications increases as well. The treatment is difficult and includes not only insulinotherapy and non-pharmacological trials. Recently treatment of insulin resistance with biguanids, and treatment with typical for type 2 new diabetes drugs like GLP-1 analogues, SGLT-2 receptor inhibitors, or even cases of bariatric surgery also has been reported.
Care pathways in forensic mental health services in New Zealand

On entering forensic mental health services (FMHS) patients progress along forensic care pathways (FCP) that aim to maximise well-being and reduce risk of reoffending. They progress through high-, medium-, and low-secure psychiatric units with changing care and security needs. There is minimal literature examining FCP or their efficacy. This retrospective study describes the characteristics of patients admitted to an acute FMHS and explores the clinical, sociodemographic, and legal factors that influence FCP progression. Most patients were referred from prison (65.1%) and presented with violent index offending (62.4%) and psychotic disorder (80.6%). Young Maori men with psychosis predominated the forensic population. Logistic regression revealed violent index offending and diagnosis were determinants of following FCP, while violent offending and longer duration of stay were predictive of FCP progression. This study found no reduction in re-hospitalisation rates or inpatient length of stay over a three year follow-up for patients that completed the FCP.
Application of the Balance Model in the Analysis of Factors Responsible for Depressive Disorders among Women in the COVID-19 Pandemic

The COVID-19 pandemic has significantly affected the lives and mental health of people around the world, and it has become clinically essential to define risk factors in order to provide adequate prevention and support. The aim of the study was to describe coping strategies in Polish women related to the COVID-19 pandemic using the balance model, one of the most important concepts of positive psychotherapy (PPT after Peseschkian since 1977). The analysis included 735 women at the mean age of 39.61 years. The survey was conducted using the questionnaire form on the website. Based on Beck’s depression test, depressive disorders were disclosed in 32.65%, and both the presence and severity of depressive syndromes were inversely correlated with age. Using a cluster analysis, three adaptation strategies could be identified, related to the different prevalence of depressive disorders. Relationships proved the most crucial area of the balance model, responsible for the effectiveness of the coping strategy. Based on the obtained results, it has to be concluded that preventive measures should primarily concern women aged < 25 years old and focus on strengthening the relationships area.
Adsorption of Cu2+ and Cd2+ on sierozem compositely modified by zwitterionic and anionic modifier

This study aimed to investigate the adsorption of heavy metal ions onto zwitterionic and anionic modified sierozem, dodecyl dimethyl betaine (zwitterionic modifier, BS) and sodium dodecyl sulfate (anionic modifier, SDS), which were used to simultaneously modify sierozems. The adsorption characteristics of Cu2+ and Cd2+ on each composite modified sierozem were studied by batch method, and the effect of temperature, pH, and ionic strength on adsorption were compared. The following results were obtained: (1) the adsorption isotherms of Cu2+ and Cd2+ in different modified sierozems followed the Langmuir model. The adsorption capacities of Cu2+ and Cd2+ were in the order of BS+SDS-modified > BS-modified > unmodified sierozem. With the increase of modified proportion of SDS, the amount of Cu2+ and Cd2+ adsorbed onto BS+SDS-modified sierozem increased. Cu2+ has a higher adsorption amount on the same modified sierozem than Cd2+. (2) The increase of temperature inhibited Cu2+ and Cd2+ adsorption in BS+SDS modified sierozems but promoted Cu2+ and Cd2+ adsorption in BS-modified and unmodified sierozems. Low ionic strength and high pH promoted the Cu2+ and Cd2+ adsorption in all tested modified sierozems. (3) The adsorption of Cu2+ and Cd2+ onto different sierozem samples showed a spontaneous, entropyincreasing, and endothermic process on CK and BS-modified sierozem and spontaneous, entropy-increasing, and exothermic process on BS+SDS-modified sierozem. The spontaneity and entropy of Cu2+ adsorption were higher than those of Cd2+.
The Effect of Visualisation Level and Situational Visibility in Co-located Digital Musical Ensembles

Digital Musical Instruments (DMIs) offer new opportu-nities for collaboration, such as exchanging sounds or sharing controls between musicians. However, in the context of spontaneous and heterogeneous orchestras, such as jam sessions, collective music-making may become challenging due to the diversity and complexity of the DMIs and the musicians’ unfamiliarity with the others’ instruments. In particular, the potential lack of visibility into each musician’s respective contribution to the sound they hear, i.e. who is playing what, might impede their capacity to play together. In this paper, we propose to augment each instrument in a digital or-chestra with visual feedback extracted in real-time from the instrument’s activity, in order to increase this awareness. We present the results of a user study in which we investigate the influence of visualisation level and situational visibility during short improvisations by groups of three musicians. Our results suggest that internal visualisations of all instruments displayed close to each musician’s instrument provide the best awareness.
The Influence Of Building And Atmosphere On The Development Of Coffee Shop Businesses In The SDGs Era Through Social Media As Intervening Variable

The purpose of this research is to analyze the variables of the coffee shop building and atmosphere directly on the social media variables, and to analyze the influence of the coffee shop building and atmosphere variables indirectly on the business development variables through social media variables. The research time is in May-July 2021 and the location is a coffee shop in Cirebon City. The research design is quantitative with a survey method. The research population is the owner of the coffee shop, amounting to 85 people so that the sampling technique is carried out by census. Measurement of data with Liker Scale and data analysis using path analysis. The results of the study concluded that the variables of the building and the atmosphere of the coffee shop had a positive effect on social media, and the building and atmosphere of the coffee shop had a positive effect on business development through social media in the coffee shop in the city of Cirebon. The results of this study are expected to be input for coffee shop owners in managing their business in this SDGs era.
The industrial management of SMEs in the era of Industry 4.0

Industry 4.0 provides new paradigms for the industrial management of SMEs. Supported by a growing number of new technologies, this concept appears more flexible and less expensive than traditional enterprise information systems such as ERP and MES. However, SMEs find themselves ill-equipped to face these new possibilities regarding their production planning and control functions. This paper presents a literature review of existing applied research covering different Industry 4.0 issues with regard to SMEs. Papers are classified according to a new framework which allows identification of the targeted performance objectives, the required managerial capacities and the selected group of technologies for each selected case. Our results show that SMEs do not exploit all the resources for implementing Industry 4.0 and often limit themselves to the adoption of Cloud Computing and the Internet of Things. Likewise, SMEs seem to have adopted Industry 4.0 concepts only for monitoring industrial processes and there is still absence of real applications in the field of production planning. Finally, our literature review shows that reported Industry 4.0 projects in SMEs remained cost-driven initiatives and there in still no evidence of real business model transformation at this time.
A life cycle for clusters? : the dynamics of agglomeration, change, and adaption

The rationale for studying cluster dynamics. - Introduction: Clusters, change and adaptation. - Literature review - The benefits of co-location. - Stability and change: Driving cluster development. - The nature of the beast - On the notion of agglomeration externalities. - Towards a complexity perspective on clusters. - Clusters, change and adaptation: Sticky places in slippery space? - Modelling adaptation in clusters - The promise of complexity theory. - Model development - Clusters as complex adaptive systems. - Micromotives and macrobehaviour - Dynamics of N/K systems. - Clusters as co-evolving N/K systems. - Division of labour, co-ordination and cluster adaptation. - Clusters, change and adaptation - Simulation results. - Model contribution, limitation and avenues for future research.
Cultural Heritage, Sense of Place and Tourism: An Analysis of Cultural Ecosystem Services in Rural Hungary

The paper focuses on three dimensions of Cultural Ecosystem Services—cultural heritage, sense of place and tourism—and examines the relationships between them in a rural landscape context. Sense of place connects to landscapes that foster authentic human attachment, cultural heritage values and elements of local history and culture. This suggests that a sense of place cannot be considered in isolation from cultural heritage. However, cultural heritage has been relatively under-researched in the context of cultural ecosystem services, where it is defined as the tangible and intangible benefits that are derived mostly from landscapes. Researchers in rural development have highlighted the importance of sense of place and cultural heritage in both place-making and tourism development. This study explores these relationships further using three case studies from rural Hungary based on in-depth interviews with local stakeholders. The findings demonstrate that cultural heritage is an inherent part of rural place-making in Hungary and that cultural heritage values strongly shape a sense of place. Social and intangible aspects of cultural heritage are the most important for place-making, especially authentic local traditions. The findings suggest that even though a strong sense of place based on cultural heritage is a pre-requisite for tourism development, initiatives have often been more successful in strengthening social cohesion and cultural identity rather than attracting tourists.