Miracle-dz/UniFAR-FTUs huggingface dataset:
Insertion of Zygomatic Implants with a Technical Modification of the Extrasinus Protocol: A Retrospective Case Series.

PURPOSE The aim of this retrospective clinical case series report was to evaluate the outcomes of patients who underwent zygomatic implant surgery with a recent technical modification of the extrasinus surgical protocol. MATERIALS AND METHODS The implant system presented in this study had a novel designed unthreaded body with a 12.5-mm sharp threaded apical end for obtaining maximum retention to the zygomatic bone. A total of 92 patients with severely atrophic maxillae were included in this study. All the patients were treated with a modification of the extrasinus protocol for insertion of 261 zygomatic implants. The mean follow-up of the patients was 34.5 ± 17.1 (SD) months (range: 6 to 72 months). The implant survival rate was the primary outcome. The intraoperative and postoperative complications were evaluated as additional criteria for success. RESULTS The cumulative implant survival rate was 97.99%. Definitive or provisional prostheses were delivered on the same day of surgery, which resulted in an improvement in the quality of life of the patients. Five implants failed in four patients. No sinusitis or mucositis was seen in any of the patients. Eleven postoperative complications occurred in seven patients. CONCLUSION The novel zygomatic surgery protocol introduced in this study can be an effective alternative to augmentation procedures and conventional implants, especially in cases of extremely atrophic posterior maxillae.
Estimated Prevalence and Incidence of Uterine Leiomyoma, and Its Treatment Trend in South Korean Women for 12 years: A National Population-Based Study.

Background: Although uterine leiomyoma causes many problems, including infertility, there are few studies that have investigated the epidemiologic characteristics of uterine leiomyoma in South Korea. The aim of this study is to estimate the prevalence and incidence of uterine leiomyoma in South Korea and analyze the treatment trends. Materials and Methods: Women of reproductive age (15-54 years) were selected from the Korean National Health Insurance Service (NHIS) sample cohort dataset, which was collected from 2002 to 2013. Patients with uterine leiomyoma were identified by ICD-10 (International Codes of Disease, 10th Edition) and intervention codes. Prevalence and incidence were calculated from the NHIS cohort dataset and the treatment trends were analyzed for diagnosed patients. Results: The prevalence in overall age groups increased from 0.96% in 2002 to 2.43% in 2013, and the 1-year incidences of all age groups increased. The 26-30 age group showed the highest rate of 1-year incidence increase (2.14-folds, 0.33% in 2003 to 0.70% in 2013). The proportion of myomectomy increased from 22% in 2002 to 49% in 2013, whereas the proportion of hysterectomy decreased from 78% to 45%. Conclusions: The prevalence and incidence of uterine leiomyoma are increasing in South Korea as time progresses, and the rate of incidence increase is higher in younger reproductive women. Overall trends in uterine leiomyoma treatment are shifting to the methods of the saving uterus.
Survival Implications of the Relationship between Tissue versus Circulating Tumor DNA TP53 Mutations—A Perspective from a Real-World Precision Medicine Cohort

Interrogating the genomics of circulating tumor DNA (ctDNA; the liquid biopsy) has advantages in patients in whom tissue biopsy is difficult. However, the reported concordance between genomic analysis of tissue DNA and ctDNA is variable among studies. Herein, we characterized the clinical implications of the relationship between mutations in TP53 genes in tissue DNA versus ctDNA. The molecular profiles of both liquid (Guardant Health) and tissue (Foundation Medicine) biopsies from 433 patients were analyzed (pan-cancer setting). In 71/433 (16%) cases, all same TP53 mutations were detected in both tissue DNA and ctDNA; in 18/433 (4%), same mutation plus additional mutation/mutations; and in 27/433 (6%), different TP53 mutations were detected. In 99/433 (23%) cases, TP53 mutations were detected only in tissue DNA; in 43/433 (10%), only in ctDNA; and in 175/433 (40%), no TP53 mutations were detected in either test. When TP53 mutations were identical in tissue and ctDNA, the alterations were enriched for nonsense mutations, and survival was significantly shorter in multivariate analysis (as compared with different mutations in ctDNA vs. tissue or no mutations); this finding was independent of tumor type, time interval between tests, and the %ctDNA for TP53 mutations. In summary, in 16% of 433 patients with diverse cancers, TP53 mutations were identical in tissue DNA and ctDNA. In these individuals, the alterations were enriched for stop–gain (nonsense) mutations (results in a premature termination codon). Though unknown confounders cannot be ruled out, these patients fared significantly worse than those whose ctDNA and tissue DNA harbored different TP53 mutation portfolios or no TP53 mutations.
Responses of Wild Birds to Novel Prey: Evidence of Dietary Conservatism

Models of the evolution of aposematic coloration assume that the first brightly coloured morphs to appear in a cryptic population of chemically defended insects would have attracted the attention of predators, putting them at a disadvantage by comparison with their less conspicuous relatives. However, if birds show a generalised avoidance response (neophobia) towards novel prey, this could allow conspicuous morphs to survive long enough to enable aposematic selection to take over. The present experiment attempted to evaluate this possibility by pre-training individually identifiable wild blackbirds (n=19) and robins (n=12) with pastry baits of a particular colour and then giving them a choice between familiar-coloured and novel-coloured baits. The number of trials taken for each bird to contact the novel colour of bait for the first time, and toeat it on a regular basis, was recorded. Birds were extremely variable in their responses: some accepted novel-coloured baits immediately while others took more than a hundred exposures before eating them regularly. Green baits were avoided for longer than red or yellow. The results suggest (a) that a new aposematically coloured morph would not necessarily suffer a higher predation rate than its cryptic ancestors, and (b) that colours that are normally considered to be aposematic on the basis of laboratory experiments may not be especially aversive to wild birds.
Frequency of α‐ and β‐haemolysin in Staphylococcus aureus of bovine and human origin

The phenotypic expression of haemolysins and the presence of genes encoding a and β‐haemolysin were determined in 105 Staphylococcus aureus isolates from bovine mastitis, 100 isolates from the nostrils of healthy humans, and 60 isolates from septicaemia in humans. Furthermore, the possible change in expression of haemolysins after subcultivation in human and bovine blood and milk was studied in selected isolates. α‐haemolysin was expressed phenotypically in 39 (37%) of the bovine isolates, in 59 (59%) of the human carrier isolates, and in 40 (67%) of the isolates from septicaemia. β‐haemolysin was expressed in 76 (72%) bovine, 11 (11%) carrier, and 8 (13%) septicaemia isolates. Significantly more bovine than human isolates expressed β‐haemolysin and significantly fewer expressed α‐haemolysin. Genotypically, the gene encoding a‐haemolysin was detected in all isolates. A significant difference in the prevalence of the gene encoding β‐haemolysin between the bovine (96%), human carrier (56%) and isolates from septicaemia (57%) was found. Of the bovine isolates, 75% of those carrying the gene encoding β‐haemolysin expressed β‐haemolysin phenotypically, whereas only 20% of the carrier isolates and 24% of the septicaemia isolates did so. No change in expression of haemolysins could be observed after subcultivation of bovine isolates in human blood and milk. After 5 to 10 subcultures in bovine blood and 1 to 4 in bovine milk, 9 of 10 human isolates originally phenotypically β‐haemolysin negative initiated the expression of β‐haemolysin. This study showed that a larger proportion of S. aureus of bovine origin carry the β‐haemolysin gene compared to isolates from humans. Furthermore, a larger number of the isolates of bovine origin carrying the β‐haemolysin gene express this gene phenotypically compared to isolates of human origin.
Ultrasonic Telemetry of Red Snapper, Lutjanus campechanus, at Artificial Reef Sites in the Northeast Gulf of Mexico

An extensive artificial reef system exists in the northeast Gulf of Mexico (Szedlmayer and Shipp, 1994), and red snapper, Lutjanus campechanus, is a major faunal component of this system. This species is actively sought by commercial and recreational fishers, but it is uncertain whether artificial reefs simply aggregate red snapper or actually increase their production (Bohnsack, 1989). Production of red snapper on artificial reefs is difficult to estimate without
Melatonin ameliorates degenerative alterations caused by age in the rat prostate and mitigates high‐fat diet damages

Imbalance of sexual steroids milieu and oxidative stress are often observed during aging and correlated to prostate disorders. Likewise, high‐fat intake has been related to prostate damage and tumor development. Melatonin (MLT) is an antioxidant whose secretion decreases in elderly and is also suggested to protect the gland. This study evaluated the impact of a long‐term high‐fat diet during aging on prostate morphology and antioxidant system of rats and tested the effects of MLT supplementation under these conditions. Male rats were assigned into four groups: control, treated with MLT, high‐fat diet and high‐fat diet treated with MLT. The high‐fat diet was provided from the 24th week of age, MLT from the 48th (100 μg/kg/day) and rats were euthanized at the 62nd week. The high‐fat diet increased body weight, retroperitoneal fatness, glycaemia, and circulating estrogen levels. It aggravated the aging effects, leading to epithelial atrophy (∼32% reduction of epithelial height) and collagen fibers increase (83%). MLT alone did not alter biometric and physiological parameters, except for the prostate weight decrease, whereas it alleviated biometric as well as ameliorated acinar atrophy induced by high‐lipid intake. Systemic oxidative stress increased, and prostatic glutathione peroxidase activity decreased fivefold with the high‐fat diet despite the indole. Regardless of the diet, MLT triggered epithelial desquamation, reduced androgen receptor‐positive cells, increased smooth muscle layer thickness (12%), decreased at least 50% corpora amylacea formation, and stimulated prostatic gluthatione‐S‐transferase activity. In conclusion, MLT partially recovered prostate damage induced by aging and the long‐term high‐fat diet and ameliorated degenerative prostate alterations.
Neurosurgery and neuromodulation for anorexia nervosa in the 21st century: a systematic review of treatment outcomes

ABSTRACT As current psychosocial and pharmacological interventions show limited efficacy in the treatment of anorexia nervosa (AN), interest in the potential value of neurosurgical intervention and neuromodulation in managing severe and enduring illness has grown. We conducted a systematic review of 20 trials of neurosurgical and neuromodulatory treatments for AN, including neurosurgical ablation, deep brain stimulation (DBS), repetitive transcranial magnetic stimulation (rTMS), and transcranial direct current stimulation (tDCS). Overall, there is evidence to support the role of stereotactic ablation and DBS in the treatment of AN. In contrast, results for rTMS and tDCS have been modest and generally more mixed. Neurosurgical treatment may offer important new avenues for the treatment of AN. Additional randomized clinical trials with comparable patient populations will be needed, in which change in affective, cognitive, and perceptual symptom phenomena, and interrogation of targeted circuits, pre- and post-intervention, are carefully documented.
[Expression of DNMT3b in human bladder cancer tissue and its correlation with clinical prognosis].

OBJECTIVE To investigate the expression of DNMT3b in human bladder cancer tissues and its correlation with postoperative survival of patients with bladder cancer. METHODS Thirty-eight pairs of surgically resected human bladder cancer tissues and adjacent bladder tissues were detected by immunohistochemistry for DNMT3b expression, and the correlations of DNMT3b expression level were analyzed with the patients'age, gender, pathological grade, tumor size, T stage, lymph node metastasis and TNM stages. Kaplan-Meier survival analysis was performed to assess the effect of DNMT3b expression on survival outcomes of the patients. RESULTS High DNMT3b protein expression was detected in 63.16% of the bladder cancer tissues and in 13.16% of the adjacent tissues (P < 0.05). The expression level of DNMT3b was associated with the pathological grade (P=0.002), tumor size (P < 0.001), T stage (P < 0.001), lymphatic metastasis (P=0.039) and TNM stage (P < 0.001), but not with gender or age of the patients. Multivariate logistic regression analysis showed that the protein expression level of DNMT3b was correlated with tumor size (P=0.008) and TNM grades of the tumor (P=0.042). Kaplan-Meier analysis showed that the patients with a high DNMT3b expression had a significantly shorter overall survival than those with a low DNMT3b expression (P=0.021). CONCLUSIONS DNMT3b overexpression in bladder cancer is closely related to such clinicopathological factors as pathological grade, tumor size, T stage, lymphatic metastasis, and TNM stage and a shorter overall survival of the patients, suggesting the potential value of DNMT3b as a prognostic marker and a new therapeutic target for bladder cancer.
Quantitative Phytochemical Evaluation of Stem Bark Extracts of Harungana madagascariensis

Aims: The study was conducted to quantify some phytochemicals present in hydroethanol (absolute ethanol: water 1:1 v/v) and methanol extracts of Harungana madagascariensis stem bark traditionally used in the management of diabetes mellitus. Methodology: Hydroethanol and methanol extracts of H. madagascariensis were separately prepared from the stem bark powder. The quantitative phytochemical analysis of the hydroethanol and methanol crude extracts were carried out by employing standard conventional protocols for total phenols, tannin, saponin, alkaloids, and anthraquinone Short Communication British Journal of Pharmaceutical Research, 4(17): 2068-2074, 2014 2069 from plants. Results: Both samples showed the presence of all phytochemicals investigated. The study revealed that hydroethanol extract of H. madagascariensis stem bark contained higher amounts of bioactive compounds (65.72%±3.36, 7.22%±0.20, 2.45%±0.42 and 0.54%±0.04) in comparison with the methanol extract (52.54%±2.35, 3.50%±0.17, 0.60%±0.05 and 0.42%±0.02) for total phenols, tannin, alkaloids, and anthraquinone respectively. Amongst these, only total phenols and tannins were significantly (P<0.05) higher. The exception to this trend was in saponin which was higher in the methanol extract (0.59%±0.06) as opposed to 0.38%±0.11 in the hydroethanol extract. Conclusion: Thus in all phytochemicals studied, hydroethanol extract of H. madagascariensis stem bark was found to be richer than the methanol extract. Saponin was the only exception. It can be concluded that bioactive compounds are more abundant in hydroethanol extract, and this potential could further be exploited in drug development.
Adipose tissue is a key organ for the beneficial effects of GLP‐2 metabolic function

Glucagon‐like peptide‐2 (GLP‐2) is a gastrointestinal hormone released in response to nutritional intake that exerts a wide range of effects by activating GLP‐2 receptors. In addition to its intestinotrophic effects, GLP‐2 also positively influences glucose metabolism under conditions of obesity, but the mechanisms behind this remain unclear. Here, we have investigated the molecular role of the GLP‐2/GLP‐2 receptor axis in energetic metabolism, focusing on its potential modulatory effects on adipose tissue.
Dietary oleuropein extract supplementation or their combination with α-tocopheryl acetate and selenium modifies free fatty acid profile and improves stability of pork.

BACKGROUND Olive-derived antioxidants have shown interesting effects on the oxidative status and have also been associated with greater consumption of glucose, which might affect glycogen stores and muscle characteristics. This study evaluated the effect of oleuropein extract supplementation (OLE) vs. vitamin E+Se (VE) or their combination (VEOLE) in pig diets on pH, drip loss, free fatty acid proportion and meat stability; and its prediction by blood oxidative status markers. RESULTS The drip loss of muscle was lower in antioxidant-supplemented groups when compared to control. α-Tocopherol concentration and total fatty acids profile were not affected by dietary oleuropein supplementation. However, OLE and VEOLE had lower free-n-3 PUFA when compared to VE and tended to have higher free-MUFA. Furthermore, VEOLE group have lower free-n-6 PUFA when compared to control or VE, whereas OLE had intermediated values. Muscle samples from pigs subjected to the antioxidant-mixed supplementation (VEOLE) had lower malondialdehyde concentration when compared to the others. The groups VE and OLE showed intermediate malondialdehyde values. Chilled meat stability was highly correlated with antioxidant status in vivo. CONCLUSION The administration of 96 mg oleuropein kg-1 feed produced similar meat quality characteristics that the use of 100 mg kg-1 α-tocopheryl acetate + 0.26 mg kg-1 sodium selenite and it would be an interesting alternative in Mediterranean countries. The VEOLE group was the most effective for reducing lipid oxidation and the production of polyunsaturated free fatty acids of meat, which would result in lower rancidity formation and better aroma development in products. This article is protected by copyright. All rights reserved.
Runoff from simulated rainfall in 2 montane riparian communities.

Riparian ecosystems are the final terrestrial zone before runoff water enters a stream. They provide the last opportunity to decrease non-point source pollution delivery to streams by removing sediments from overland water flow from uplands and roads. To quantify processes of sediment transport, filtration and deposition, it is necessary to determine runoff characteristics for the area. A rotating boom rainfall simulator was used to evaluate the effects of 3 vegetation height treatments (control, 10-cm stubble height, and clipped to the soil surface) in 2 montane riparian plant communities (grass and sedge) on runoff characteristics. Each rainfall simulation event consisted of 2 phases, a dry run of about 60 min followed by a wet run approximately 30 min later. There were no differences in time to runoff initiation for either dry or wet runs that could be attributed to vegetation height treatments for either plant community. It usually required more time for runoff to be initiated in the sedge community compared to the grass community. Generally, there were lower equilibrium runoff percentages from dry runs in the sedge community compared with the grass community. These differences were less during wet runs. Several runoff parameters had characteristics of runoff from water repellent soils. The organic layer on the soil surface exhibited signs of water repellency that reduced the water infiltration rate during the initial stages of a rainfall simulation. These results indicate that runoff and infiltration processes in the surface organic horizon of riparian zones may not respond in the classical manner. This characteristic has important implications if criteria developed in areas with less organic matter on the soil surface are used to manage overland flow in the zone. Additional studies are needed to fully describe infiltration and runoff processes in riparian plant communities.
Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon–anticodon pairing

Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (m1G37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of m1G37 in tRNAPro causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNAPro bound to either cognate or slippery codons to determine how the m1G37 modification prevents mRNA frameshifting. The structures reveal that certain codon–anticodon contexts and the lack of m1G37 destabilize interactions of tRNAPro with the P site of the ribosome, causing large conformational changes typically only seen during EF-G-mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how m1G37 stabilizes the interactions of tRNAPro with the ribosome in the context of a slippery mRNA codon.
Impact of Atopic Dermatitis in Adolescents and their Parents: A French Study

Atopic dermatitis has a negative impact on quality of life in patients and their families. However, there have been very few studies of the impact of atopic dermatitis on adolescents and their relatives. The objective of this study was to evaluate the impact of atopic dermatitis in the daily lives of adolescents between 12 and 17 years of age in the French population and to assess the burden of the disease on their families. Quality of life was measured in 399 parents of adolescents with atopic dermatitis and in the adolescents themselves. Impairment of quality of life in the adolescents was associated with disease severity. Moreover, in children aged 12–14 years, quality of life was worse with increasing age, with decreasing disease duration, and when parents had atopic dermatitis. In children aged 15–17 years quality of life was worse when the parent who answered the questionnaire was male and when the parent was <45 years old. The burden of atopic dermatitis was higher in parents of older children, in parents with children with higher disease severity, with shorter disease duration, in male parents, and in parents aged < 45 years. The burden of atopic dermatitis in adolescents and their parents is considerable and should be taken into account in the management of atopic dermatitis.
Inhibition and success of prymnesium parvum invasion on plankton communities in Texas, USA and prymnesium parvum pigment dynamics

Inhibition and Success of Prymnesium parvum Invasion on Plankton Communities in Texas, USA and Prymnesium parvum Pigment Dynamics. (May 2006) Reagan Michelle Errera, B.S., Trinity University Chair of Advisory Committee: Dr. Daniel Roelke Prymnesium parvum Carter, a haptophyte species capable of forming harmful algal blooms (HABs), has been identified in fresh and brackish water habitats worldwide. In Texas, P. parvum blooms have diminished local community revenues from losses to tourism, fishing, and hatchery production. In this thesis, P. parvum dynamics were studied using in-situ microcosm experiments at Lake Possum Kingdom, Texas during three seasons (fall, winter, spring) in 2004-2005. Specifically, nutrient additions were used to test the hypothesis that increased nutrient levels would not enhance P. parvum’s ability to invade phytoplankton communities. In addition to full nutrient additions to levels of f/2 media, other treatments included nutrient additions deficient in either nitrogen (N) or phosphorus (P). Additionally, barley straw extract was tested as a growth inhibitor to prevent P. parvum blooms. Furthermore, P. parvum initial population density was examined to test the hypothesis that increased initial populations could promote an increase in P. parvum population densities.