Miracle-dz/UniFAR-FTUs huggingface dataset:
Possible Interacting Proteins for Retinol Binding Protein and Cellular Retinol Binding Protein

Retinol or vitamin A is crucial for many biological processes in the body such as proliferation, differentiation and reproduction. It is mainly stored in the liver and can be broken down to retinoic acid inside the cell. It travels in the circulatory system attached to Retinol Binding Protein (RBP) and its transport into the cell is mediated by the RBP receptor STRA6. Inside the cell, retinol binds to cellular Retinol Binding Protein (CRBP) and can be converted to retinoic acid, which can then act as a regulator of transcription. High levels of RBP have been implicated in type 2 diabetes. Looking for possible interacting partners for proteins such as RBP, can further elucidate the function of the protein and its possible role in type 2 diabetes. The Membrane Yeast Two Hybrid (MYTH) system allows for the study of possible interacting partners for membrane proteins. Previous studies using (MYTH) looked at possible interacting partners for RBP and CRBP. Two hits for RBP were G-protein coupled receptors (GPCRs) RAIG2 and RAIG3. One hit for CRBP was the TRPC4 ion channel. Retinoic acid inducible gene (RAIG) is a recently discovered family C GPCR and the TRPC4 channel is a membrane ion channel responsible for calcium and sodium ion influxes into the cell. An RBP-RAIG interaction could implicate the RAIG receptor in type 2 diabetes. A CRBP-TRPC4 interaction could activate the ion channel causing a calcium influx and a possible mechanism of insulin resistance in the cell, the genesis of which is still very controversial. This thesis involves performing a series of pull down assays using a novel oil-based assay to determine if the proteins in the yeast two hybrid system are indeed interacting.
In vitro induction of shoots and roots from Jatropha curcas L. explants

Summary An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants. Shoot regeneration from apical shoots, nodes, axillary bud-derived shoots, petioles and leaf explants were assessed on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) alone, or in combination with indole-3-yl-butyric acid (IBA). MS medium with 2.22 – 4.44 µM BA was effective for apical shoot culture, while 4.44 µM BA was suitable for node culture. MS medium with 2.22 µM BA and 0.049 µM IBA provided the best shoot proliferation from axillary bud-derived shoots. For petiole segments from leaves at the second, third and fourth nodes, the most effective growth regulator combination for callus induction during the first culture cycle, and subsequent shoot formation during the second culture cycle, was MS medium supplemented with 4.44 µM BA and 2.46 µM IBA. A combination of 8.88 µM BA and 4.90 µM IBA was suitable for callus induction and shoot formation from leaf segments at the second and third nodes of branches. For rooting, 4.90 µM IBA induced 85.71% rooting from shoots on basal rooting medium. The in vitro plantlets were acclimatised and transferred to potting mix, with 82% survival.
Standardization of method and time of budding in pecan.

The experiment was conducted to standardize grafting technique in pecan. Three grafting methods were performed on 3 different dates. Tongue grafting gave highest success compared to cleft and crown grafting. Highest bud sprouting and survival rate were recorded in tongue grafting performed on 3rd March followed by the same method performed on 15th February. Cleft graft on 18th March was the earliest to sprout. The proportion of saleable plants was highest in tongue grafting (71.33%) followed by cleft grafting (61.44%).
A hospital based cross sectional study to evaluate awareness of lifestyle interventions among hypertensive patients in Sikkim (North-Eastern State of India)

Introduction: Hypertension (HTN) or high blood pressure (BP) is the most important cardiovascular risk factor worldwide, contributing to around one half of the coronary heart disease and two thirds of the cerebrovascular disease burdens. Effective prevention, detection, treatment, and control of BP continue to be an important goal for health care providers. A positive impact of life-style interventions is a well known fact. Awareness of HTN remains an important concern in this mountainous and predominantly rural population. Objective: To evaluate the awareness of lifestyle interventions among patients of hypertension. Methods: A cross-sectional study was conducted in Sikkim Manipal Institute of Medical Sciences in general medicine OPD from Jan 1. 2017 to Jan 15, 2017. All the stable hypertensive patients, attending medicine OPD aged >18 years were recruited in the study. Patients were interviewed using a semi structured questionnaire regarding knowledge of life style interventions. Results: Among all the hypertensive patients (n = 100), 60 patients had adequate knowledge (>50%) and 40 patients had inadequate (<50%) knowledge of these interventions. Significant association between educational background and knowledge on lifestyle interventions was present. Urban population was more aware as compared to rural population Blood pressure was significantly under control in the aware population. Conclusion: Patients knowledge on lifestyle interventions for the management of blood pressure is important. The rural population and uneducated people have inadequate knowledge on lifestyle modifications of hypertension. Structured teaching programs are needed to improve awareness about the lifestyle changes.
PENGARUH PENGELOLAAN BARANG MILIK NEGARA TERHADAP KUALITAS LAPORAN KEUANGAN PEMERINTAH ACEH (Studi Pada Dinas Pendapatan dan Kekayaan Aceh)

Penelitian ini bertujuan untuk mengetahui sejauh mana pengaruh pegelolaan kekayaan milik negara terhadap kualitas laporan keuangan (Studi Pada Dinas Pendapatan dan Kekaayaan Aceh). Penelitian ini menggunakan metode kuantitatif asosiatip, yaitu mengetahui hubungan variable independen Penata Usahan barang Milik Negara dengan variable dependen Kualitas laporan Keuangan. Sampel digunakan 25 orang responden, dengan metoded sensus. Analisa data dilakukan dengan menggunakan regresi linier sederhana dengan alat bantu SPSS 17. Dari hasil analisa selanjutnya dirumuskan kesimpulan. Hasil penelitian menunjukkan bahwa Penatausaahaan Barang Milik Negara berpengaruh positip dan signifikan terhadap kualitas Pemerintah Aceh . Hasil uji- t menunjukkan bahwa Hasil uji thitung = 7.326> tabel (2,2167), tingkat signifikan 0,000 lebih kecil dari 0.05 (5%). Disamping itu, hasil uji R pada Model Summary menunjukkan sebesar 0.700 atau 70 %, , yaitu diatas 50 %, jadi pengaruhnya positip dan relatif kuat.
Analysis of Saccharide Binding to Artocarpus integrifolia Lectin Reveals Specific Recognition of T-antigen (\beta D-Gal(1\rightarrow 3)GalNAc

The binding of Artocarpus integrifolia lectin to N-dansylgalactosamine (where dansyl is 5-dimethylaminonaphthalene-1-sulfonyl) leads to a 100\% increase in dansyl fluorescence with a concomitant blue shift in the emission maximum by 10 nm. This binding is carbohydrate-specific and has an association constant of 1.74 \times $10^4 M^{-1}$ at $20^oC$. The lectin has two binding sites for N-dansylgalactosamine. The values of -\Delta H and -\Delta S for the bindinogf N-dansylgalactosamine are in the range of values reported for severa lectin monosaccharide interactions, indicating an absence of non-polar interaction of the dansylmoiety of the sugar with the combining region of the protein. Dissociation of the bound N-dansylgalactosamine from its complex with the lectin and cosequent change in its fluorescence on addition of nonfluorescent sugars allowed evaluation of the association constant for competing ligands. The thermodynamic parameters for the binding of monosaccharides suggest that the OH groups at C-2, C-3, C-4, and C-6 in the D-galactose configuration are important loci for interaction with the lectin. The acetamido group at C-2 of 2-acetamido-2-deoxygalactopyranose and a methoxyl group at C-1 of methyl-\gamma -Dgalactopyranoside are presumably also involved in binding through nonpolar and van der Waals' interactions. The T-antigenic disaccharide Gal\beta 1\rightarrow 3GalNAc binds very strongly to the lectinw hen compared with methyl-\beta -D-galactopyranoside, the \beta ( 1\rightarrow 3)-linked disaccharides such as Gal\beta 1\rightarrow 3GalNAc, and the \beta (1\rightarrow 4)- linked disaccharides, N-acetyllactosamine andla ctose. The major stabilizing force for the avid binding of Tantigenic disaccharide appears to be a favorable enthalpic contribution. The combining site of the lectin is, therefore, extended. These data takent ogether suggest that the Artocarpus lectin is specific toward the Thomsen-Friedenreich (T) antigen. There are subtle differences in the overaltlo pography of its combining site when compared with that of peanut (Arachis hypogaea)agglutinin.The results of stopped flow spectrometry for the binding of N-dansylgalactosamine to the Artocarpus lectin are consistent with a simple single-step bimolecular association and unimolecular dissociation rate processes. The value of $K_{+1}$ and $K_{-1}$, at $21 ^oC$ are 8.1 X $10^5 M^{-1} s^{-1}$ and 50 $s^{-1}$, respectively. The activation parameters indicate an enthalpy-controlled association process.
Polyphenolic Content and Antioxidant Capacity in Fruits of Plum (Prunus domestica L.) Cultivars “Valjevka” and “Mildora” as Influenced by Air Drying

Polyphenolic content and antioxidant capacity of freshly harvested plums cvs. “Valjevka” and “Mildora,” and changes caused by drying were analyzed. Plum drying at 90C resulted in significant changes in anthocyanins, flavonoids and phenolics content, and antioxidant capacity in both cultivars examined. Statistical analysis showed that antioxidant capacity of both fresh plums and prunes of “Valjevka” and “Mildora” is strongly influenced by the phenolic constituents of the fruit. The major phenolic compound in fresh plums and prunes is neochlorogenic acid, followed by caffeic acid and chlorogenic acid. After drying, a significant decrease in neochlorogenic acid and an increase in caffeic acid was observed, while chlorogenic acid content decreased in prunes of “Valjevka,” and increased in prunes of “Mildora. ” Rutin and protocatechuic acid contents were slightly decreased after drying, while gallic acid content was dramatically increased. A complete degradation of cyanidin was induced by drying. Practical Applications This study is intended to inform producers of dried fruits about changes induced by drying, as well as to highlight the supposition that dried fruits may be considered functional food due to the high level of polyphenolic compounds and increased antioxidant capacity. The scientific results relative to the nutritional profile of prunes, combined with their attractive appearance and favorable flavor, may serve as a commercial strategy, given the increased level of consumers' awareness on the importance of healthy food intake.
Population genetics and genetic conservation of forest trees.

Cet ouvrage presente les resultats des divers projets de recherche menes ces cinq dernieres annees en matiere de diversite et de gestion des ressources genetiques des arbres forestiers. Il donne un apercu des orientations de la recherche qui permettent une meilleure aprehension de la diversite genetique ainsi que des strategies de conservation envisagees pour le futur. Les differentes communications sont regroupees en cinq sections : - role des differents types de marqueurs dans l'evaluation des niveaux de la variabilite genetique, - shemas de la variabilite genetique en fonction des origines geographiques, - relations entre les shemas de selection et la dispersion genetique, - relations entre la structuration de la diversite genetique et la strategie de conservation des ressources genetiques et exemples d'actions engagees concernant les mesures de sauvegarde de la diversite genetique
The effect of selection for intramuscular fat on fatty acid composition in Duroc pigs

27 Introduction 28 Materials and Methods 29 Results and Discussion 33 Literature Cited 39 CHAPTER 4. THE EFFECT OF GENDER ON FATTY ACID 54 COMPOSITION, MEAT QUALITY AND SENSORY CHARACTERISTICS IN DUROC PIGS SELECTED FOR INTRAMUSCULAR FAT CONTENT FOR SEVEN GENERATIONS Abstract 55 Introduction 56 Materials and Methods 57 Results and Discussion 61 Literature Cited 6655 Introduction 56 Materials and Methods 57 Results and Discussion 61 Literature Cited 66
Frequency distribution of equine diseases in three metropolises of the Upper Punjab, Pakistan.

We developed an inventory of the diseases of equines prevalent in three districts (Faisalabad, Sargodha and Lahore) of Punjab, Pakistan affecting the equine health and production. Survey was done with the help of Brooke Hospitals for Animals, Pakistan, located at the University of Agriculture, Faisalabad, Pakistan. Participatory epidemiological approach was used to collect information on a pre-designed quesntionnaire from urban and peri-urban areas of the study districts. A total of 450 equines including: 147 horses, 230 donkeys and 73 mules were subjected to clinical examination, blood examination and necropsy for the documentation of diseases/conditions of equines. A total of 53 diseases/conditions were documented in different species of equines in the study area. Internal parasites (32.2%) constituted the major category of ailments followed by wounds (26.9%), bacterial infections (20.4%), lameness (12.9%), miscellaneous (12.7%), gastrointestinal disorders (7.6%), bronchitis/cough (7.3%), allergic dermatitis (7.1%), external parasites (6.2%), colic (5.3%), eye problems (3.1%), hematuria (1.8%) and quidding (1.1%). Horses were most diversely affected (n=47/53) followed by donkeys (n=44/53) and mules (n=24/53). This information may serve as a guidline for planning an equine disease control programme in the targeted districts of the province. © 2013 Friends Science Publishers
Effect of bioregulators and zinc on growth and yield of okra (Abelmoschus Esculentus L. Moench.)

The present experiment was conducted to study the response of soil application of Zn and seed soaking and or foliar spray of thiourea and kinetin on okra. The increasing levels of zinc upto 5.0 kg ha1 brought substantial improvement in all characters (plant height, number of branches, number of leaves, main stem girth and chlorophyll content of leaf), yield attributes (fruit length, fruit diameter, number of fruits per plant, fruit weight and yield per plant), fruit yield (192.79qha−1). The treatment 500 ppm thiourea seed soaking+ 500 ppm thiourea sprays at vegetative and flowering stages provided higher values of all growth characters (plant height, no. of branches, no. of leaves, main stem girth and chlorophyll content of leaf), yield attributes (fruit length, fruit diameter, No.of fruits/plant, fruit weight and yield/plant) fruit yield (191.08 q ha−1).
Retroviral Pseudotypes – From Scientific Tools to Clinical Utility

Retroviral pseudotypes are important research and diagnostic tools for basic and clinical virology studies, facilitating the detailed investigation of individual genes, cellular receptors, antibody responses, serosurveillance and antiviral therapies. Importantly, pseudotypes enable the study of highly pathogenic viruses, without the need for high containment. Their use as gene therapy vectors is widely documented, but other uses, once less well known, are becoming more prominent. The substitution of envelope proteins expressed on the virion surface enables pseudotypes to be employed as surrogates for wildtype viruses in antibody neutralisation or antiviral screening assays and for the study of cell–virus receptor interactions. In addition, they are increasingly being utilised as vaccine immunogens, expressing the antigen either on the particle surface or as a transfer gene for cellular expression. These studies demonstrate the potential for using pseudotypes for both scientific and clinical applications.
Developing white spot lesion (WSL) in patients with Fixed orthodontic appliance in Erbil city of Iraq

AIMS: of this cross sectional study is to specify the occurrence of white spot lesion (WSLs) in patients with orthodontic attachment in Erbil, Iraq. MATERIALS AND METHODS: This cross-sectional study was carried out on 20 subjects (7 male – 13 female) their age ranged between 12-34 years old. During fixed orthodontic treatment each tooth in four quadrants was examined by naked eye to detect the occurrence of WSLs from the 1 month to 6 month of treatment duration. RESULTS: The prevalence of WSL according to age, gender, and duration of the orthodontic treatment. The mean age of male were 19.43 (S.D= 6.37) and the mean age of female were 19.00 (S.D= 7.37) ; but statistically calculated nonsignificant (P-value >0.05). In the first visit the mostly affected teeth are upper right canine, upper left canine, and upper left lateral incisor, In second visit the most affected teeth are upper left canine, upper left lateral incisor, and lower right canine. In the third visit the mostly affected teeth are upper left canine, upper left lateral incisor, and lower right canine, In the fourth visit the most affected teeth are upper left canine and upper left lateral incisor. The frequency of WSLs increased with each visit of orthodontic treatment, the maximum occurrence of WSLs was found in the sixth visit (21.25%) followed by fifth visit (21%), fourth visit (19.25%), third visit (16.25%), second visit (13.75%), and finally first visit (7.25%). The mean of all visit equal to 16.45%. CONCLUSION: 21.25% of patients in developing WSLs during orthodontic treatments were more attend in the upper than lower arch; they occurred most often on the upper left canine and upper left lateral incisor. There was statistically no significant male and female difference, females had a 65% of developing WSLs than males 35% (P-value <0.05).
Some Eco-Physiological Characteristics at R4-R5 Stage in Relation to Soybean Yield Differing in Maturities

Digital plant canopy imager and photosynthesis analyzer system were used to analyze the characteristics of canopy structure, photosynthetic physiology and micro-environmental factors at R4-R5 stage in different yielding soybean cultivars or lines with different maturities. The results showed that the common characteristics of high yielding soybean cultivars were high LAI, uniform foliage distribution in all horizontal directions, less variance of photosynthetic rate between top and bottom leaves in canopy and relatively higher photosynthetic rate of the whole canopy. All these characters combined in all resulted in higher canopy photosynthetic productivity, and pod number, seed number and seed weight per plant, especially pod and seed number in top and middle canopy were increased. The characters of high yielding canopy varied among maturities. Light interception was more important to early cultivars. Less foliage inclination angle was benefit to intercept more solar energy during yield formation. As late soybean cultivars had a more closure canopy and higher LAI, greater foliage inclination angle in all layers of high yielding canopy made more solar radiation penetrate into canopy, which was beneficial to yield formation.
PERAN PENYULUH TERHADAP PENGUATAN KELOMPOK TANI DAN REGENERASI PETANI DI KABUPATEN BOGOR JAWA BARAT

Penelitian bertujuan menganalisis peran penyuluh terhadap penguatan, kemandirian kelompok tani serta regenerasi petani dan menganalisis pengaruh penguatan, kemandirian kelompok tani terhadap regenerasi petani. Penelitian dilakukan di wilayah kerja Balai Penyuluhan Pertanian, Perikanan dan Kehutanan Caringin, Kabupaten Bogor pada Juni sampai November 2017. Populasi penelitian adalah petani muda yang menjadi anggota kelompok tani dan gabungan kelompok tani sebanyak 60 orang yang diambil menggunakan teknik acak sederhana. Data diambil menggunakan kuesioner dengan skala instrumen rating scale. Variabel penelitian terdiri atas peran penyuluh pertanian (X1), penguatan kelompok tani (X2), kemandirian kelompok tani (X3) dan regenerasi petani (Y). Analisis data menggunakan statistik deskriptif, korelasi dan regresi. Hasil penelitian disimpulkan bahwa peran penyuluh pertanian berpengaruh signifikan terhadap penguatan kelompok tani. Peran penyuluh dan penguatan kelompok tani berpengaruh nyata terhadap kemandirian kelompok tani. Peran penyuluh, penguatan kelompok dan kemandirian kelompok tani berpengaruh tidak nyata terhadap regenerasi petani.
HYBRIDS VARIATION AND WHEAT STRAW MULCH EFFECTS ON VEGETATIVE GROWTH AND FRUIT YIELD OF SWEET PEPPER UNDER EL-ARISH AREA CONDITIONS

Field experiments were carried out during the two summer seasons of 2016 and 2017 at The Experimental Farm, Faculty of Environmental Agricultural Sciences, Arish University, North Sinai, Egypt to study the effect of mulch (without mulch and straw mulch) on two colored sweet pepper hybrids (“Yellow Star F1” and “Red Star F1”, Capsicum annuum L.). Transplanting was carried out on 19May. Straw mulch was added after 10 days later. The experimental plot area was 15m (15 m long and 1.0 m wide). Drip irrigation system was used. Each experimental plot had one dripper line, the distance between plants in the same row was 50 cm, which gave, planting density of 2.0 plants m. Split -plot design with three replicates was used, where sweet pepper hybrids were randomly arranged in the main plots, and mulch treatments were randomly arranged in the sub plots. ‘Red Star F1’ hybrid recorded the highest values of plant height, number of leaves and leaf area as well as plant fresh and dry weight traits at 75 days after transplanting in both seasons. Red Star F1’and Yellow Star F1’ both with mulch recorded the highest values of all vegetative growth traits and marketable yield traits in both seasons. Mulch treatment had higher values of all studied marketable yield traits (number of fruits/plant, mean fruit weight (g), and yield/plant (kg) in both seasons. Using straw mulch increased number of fruits/plant by 48.59% as compared to without mulch treatment. The highest values of all marketable yield traits in both seasons were recorded with ‘Red Star F1’ or ‘Yellow Star F1’ both with mulch.
Growing conditions and quality of vegetables III. Effects of light and nutritional conditions on the ascorbic acid content of lettuce.

Effects of light and nutritional conditions on the ascorbic acid content in hydroponically grown lettuce of butterhead type (Luctuca sativa L. cv. Edogawa or Okayama) were investigated. With increasing levels of shading, the fresh weight and number of leaves decreased, the shape of leaves became narrower and ascorbic acid and sugars content decreased markedly, while nitrate nitrogen content inclined to increase. Plants grown in 1/4 strength standard nutrient solution were inferior in fresh weight and number of leaves to those grown in 1/2 strength solution. Though ascorbic acid and sugars content was higher for plants grown in 1/4 strength solution at higher light intensities, the effect of nutrient concentration gradually disappeared with increasing shading level.Diurnal changes in leaf constituents were studies on sunny and cloudy days. The contents of ascorbic acid, sugars and chlorophyll were higher in the daytime and lower during the night, while nitrate nitrogen content increased in the night time. These tendencies were more clear on the sunny day than on the cloudy day.The relations between the contents of ascorbic acid, sugars, nitrate nitrrogen etc. were discussed and possible techniques of practical use for improving the quality of hydroponically grown leaf vegetables were also discussed.