Miracle-dz/UniFAR-FTUs huggingface dataset:
Mitigating effect of perceived organizational support on stress in the presence of workplace ostracism in the Pakistani nursing sector

Background Nursing has a reputation for being a predominantly stressful profession. Prior studies focus on the overt antecedents of stress like bullying, harassment, and verbal aggression from patients as well as colleagues. Employee stress has been receiving attention for decades, yet there is a research gap on the role of workplace ostracism as an antecedent of stress for nurses. This study aimed to consider the effect of workplace ostracism on the perceived stress of nurses while considering the moderating role of perceived organizational support. Methods This study is quantitative. A time-lagged survey was conducted in private and public hospitals of Pakistan. Data were collected from 241 nurses. Statistical Package for the Social Sciences (SPSS) software and analysis of a moment structures (AMOS) software were employed for data analysis, such as bootstrapping analysis, Sobel test, and regression analysis. Results Results demonstrated that workplace ostracism is positively associated with stress, while perceived organizational support has a moderating relationship. Perceived organizational support mitigates the adverse effects of workplace ostracism on nurses. Conclusion This study contributes significantly to nursing literature by identifying workplace ostracism as one of the significant antecedents of stress for nurses. Perceived organizational support shows that employees are cared for and appreciated by the organization, which lessens the strength of perceived stress due to workplace ostracism.
Chromatic pupilloperimetry for objective diagnosis of Best vitelliform macular dystrophy

Purpose: To determine the pupil response of Best vitelliform macular dystrophy (BVMD) patients for focal blue and red light stimuli presented at 76 test points in a 16.2 ° visual field (VF) using a chromatic pupilloperimeter. Methods: An observational study was conducted in 16 participants: 7 BVMD patients with a heterozygous BEST1 mutation and 9 similar-aged controls. All participants were tested for best-corrected visual acuity, chromatic pupilloperimetry and Humphrey perimetry. Percentage of pupil contraction (PPC), maximal pupil contraction velocity (MCV) and latency of MCV (LMCV) were determined. Results: The mean PPC and MCV recorded in BVMD patients in response to red stimuli were lower by . 2 standard errors (SEs) from the mean of controls in 47% and 43% of VF test points, respectively. The mean PPC and MCV recorded in the patients in response to blue stimuli were lower by . 2 SEs from the mean of controls in 36% and 24% of VF test points, respectively. The patients’ mean and median MCV recorded in response to red light correlated with their Humphrey mean deviation score ( r =− 0.714, P = 0.071 and r =− 0.821, P = 0.023, respectively) and visual acuity ( r = 0.709, P = 0.074 and r = 0.655, P = 0.111, respectively). A substantially shorter mean LMCV was recorded in BVMD patients compared to controls in 54% and 93% of VF test points in response to red and blue light, respectively. Receiver operating characteristic analysis for LMCV in response to red light identified a test point at the center of the VF with high diagnostic accuracy (area under the curve of 0.94). Conclusion: Chromatic pupilloperimetry may potentially be used for objective noninvasive assessment of rod and cone cell function in different locations of the in
Yeast Species in the Oral Cavities of Older People

6 Acknowledgement . 8 Oral and Poster Presentations . 10 List of Tables . 11 List of Figures . 13 List of Abbreviations . 14 Chapter 1: Introduction . 17 1.
Sustained effectiveness and cost-effectiveness of Counselling for Alcohol Problems, a brief psychological treatment for harmful drinking in men, delivered by lay counsellors in primary care: 12-month follow-up of a randomised controlled trial how this you. Your story matters

Background Counselling for Alcohol Problems (CAP), a brief intervention delivered by lay counsellors, enhanced remission and abstinence over 3 months among male primary care attendees with harmful drinking in a setting in India. We evaluated the sustainability of the effects after treatment termination, the cost-effectiveness of CAP over 12 months, and the effects of the hypothesized mediator ‘readiness to change’ on clinical outcomes.
Interpersonal life stress, inflammation, and depression in adolescence: Testing Social Signal Transduction Theory of Depression

Depression rates increase markedly for girls across the adolescent transition, but the social‐environmental and biological processes underlying this phenomenon remain unclear. To address this issue, we tested a key hypothesis from Social Signal Transduction Theory of Depression, which posits that individuals who mount stronger inflammatory responses to social stress should exhibit greater increases in depressive symptoms following interpersonal life stress exposure than those who mount weaker inflammatory responses to such stress.
Upregulation of microrna-1270 suppressed human glioblastoma cancer cell proliferation migration and tumorigenesis by acting through WT1

Background: Glioblastoma multiforme (GBM) is one of the most aggressive brain tumors among human beings. In this study, we explored the functions of human microRNA-1270 (hsa-miR-1270) on GBM cancer cell proliferation, migration, and tumorigenesis. Materials and methods: In GBM cell lines and clinical tissues, hsa-miR-1270 expression was probed by quantitative real-time PCR (qRT-PCR). In LN-18 and A172 cells, hsa-miR-1270 was upregulated by lentiviral transduction. The effects of hsa-miR-1270 upregulation on GBM in vitro and in vivo functions were probed by proliferation, migration, and xenograft assays, respectively. The correlation between hsa-miR-1270 and Wilms’ tumor gene ( WT1 ) was probed by dual-luciferase activity assay, qRT-PCR, and Western blot. WT1 was then secondarily over-expressed in hsa-miR-1270-upregulated LN-18 and A172 cells, to explore its mechanisms in GBM’s association with hsa-miR-1270. Results: Hsa-miR-1270 was significantly downregulated in both GBM cell lines and clinical tumors. Upregulating hsa-miR-1270 considerably suppressed GBM cell proliferation and migration in vitro and xenograft in vivo. WT1 was inversely correlated with hsa-miR-1270 in GBM. WT1 overexpression in hsa-miR-1270-upregulated GBM cells reversed the anticancer functions of hsa-miR-1270 on cancer proliferation and migration. Conclusion: Hsa-miR-1270 upregulation may have suppressing effects on GBM cancer cells, likely by functionally acting through WT1.
a case report for severe hand–foot skin reaction caused by chemotherapy with actinomycin D in a patient with oculocutaneous albinism

: Gestational trophoblastic neoplasms (GTN) are highly curable tumors, with an overall patient survival of 90%, due to the individualized chemotherapy. However, chemotherapy regimens vary between different treatment centers and the comparable benefits and risks of these different regimens are unclear. Here, we reported a case of GTN with oculocutaneous albinism (OCA) is resistant to fluorouracil (5-FU), extremely sensitive to actinomycin D (Act-D) with severe hand–foot skin reaction (HFSR). We hypothesized that the known, or unknown, gene mutations might be correlated with drug resistance, supersensitivity and severe drug side effects in OCA patients. Thus, we considered that OCA related genes influence some drug sensitivity and that the absence of melanin likely contributes to some drug resistance. It is important to assess the OCA related gene mutations locus of drug sensitivity, and resistance in OCA patients in future research.
Suspended sediment and water fluxes in Menggatal River mouth, Kota Kinabalu

Studies of suspended sediment and water flux have been conducted on 22-23 January and 26-27 February 2007. This study was conducted during one tidal cycle in Menggatal River mouth. Menggatal River mouth is situated at west bay of Kota Kinabalu and one of the main inlets into Sepanggar Bay. The objectives of this study are to identify the suspended concentration during tidal changes, to observed total water flux and sediment flux during flood tide and ebb tide in Menggatal River. Water samples were collected using water sampler and the samples were analyzed in the laboratory. The result of this study showed that the time average water velocities during flood tide were higher than ebb tide. Suspended sediment concentration in Menggatal River was higher during ebb tide than flood tide and water flux was higher during flood tide than ebb tide. Sediment fluxes were slightly different during flood tide and ebb tide.
Autoimmune Cytopenias in Chronic Lymphocytic Leukemia

The clinical course of chronic lymphocytic leukemia (CLL) may be complicated at any time by autoimmune phenomena.The most common ones are hematologic disorders, such as autoimmune hemolytic anemia (AIHA) and immune thrombocytopenia (ITP). Pure red cell aplasia (PRCA) and autoimmune agranulocytosis (AG) are, indeed, more rarely seen. However, they are probably underestimated due to the possible misleading presence of cytopenias secondary to leukemic bone marrow involvement or to chemotherapy cytotoxicity. The source of autoantibodies is still uncertain, despite the most convincing data are in favor of the involvement of resting normal B-cells. In general, excluding the specific treatment of underlying CLL, the managementof these complications is not different from that of idiopathic autoimmune cytopenias or of those associated to other causes. Among different therapeutic approaches, monoclonal antibody rituximab, given alone or in combination, has shown to be very effective.
Active B 12 assay for diagnosing vitamin B 12 deficiency

The Abbott ARCHITECT Active-B12 assay is a test for detecting levels of serum holotranscobalamin, which is the metabolically available component of vitamin B12, and can be used as a marker of vitamin B12 deficiency. Four diagnostic test accuracy studies, using different reference standards, reported greater diagnostic accuracy for the Active-B12 assay compared with assays measuring other markers of vitamin B12 deficiency. The assay needs less sample preparation than a total vitamin B12 test and the expected cost is about £3.50 per test including VAT, depending on sample throughput.
KRAS rs7973450 A>G increases neuroblastoma risk in Chinese children: a four-center case-control study

Background: Neuroblastoma is one of the most common extracranial solid pediatric tumors. KRAS plays an important role in regulating cell proliferation, differentiation, and apoptosis. Single nucleotide polymorphisms (SNPs) in KRAS have been shown to modify susceptibility to multiple tumors, but no speci ﬁ c molecular epidemiology study was reported regarding neuroblastoma. Methods: We conducted a four-center case-control study to explore the association between KRAS gene polymorphisms (rs12587 G>T, rs7973450 A>G, rs7312175 G>A) and neuroblastoma susceptibility with 505 Chinese children and 1070 matched controls. Results: We found that rs7973450 A>G was associated with signi ﬁ cantly increased neuroblastoma risk [GG vs. AA: adjusted odds ratio (OR)=4.26, 95% con ﬁ dence interval (CI) =1.28 – 14.19, P =0.018; GG vs. AA/AG: adjusted OR=4.27, 95% CI=1.28 – 14.24, P =0.018]. The strati ﬁ ed analysis further demonstrated that rs7973450 GG genotype carriers had a higher risk to develop neuroblastoma in the subgroups of males, tumor originated from the adrenal gland and clinical stages III+IV. Conclusions: Overall, our results suggested that rs7973450 A>G was associated with increased neuroblastoma risk.
The impacts of tourism.

The Key Citations series was developed to provide a starting point for persons new to the various fi elds of impact assessment. The references provided are an indicative overview of the fi eld and establish what might be regarded as the core literature. They include a selection of currently available textbooks published by commercial publishers, a selection of journal articles from the last 10 or so years, and key offi cial documents. Some historically signifi cant articles are also included. The means of determining key citations generally include consulting Scopus and Google Scholar and expert practitioners in the fi eld. IAIA members contributing to this series acknowledge possible personal and regional bias and much diffi culty in selecting only a few from among many excellent references in their fi elds. KEY CITATIONS SERIES
The combined presence of hypertension and vitamin D de�ciency increased the probability of the occurrence of small vessel disease in China

Background: The exact relationship between 25-hydroxyvitamin D [25(OH) D] levels and small vessel disease (SVD) are not clear in China. The aim of this study was to determine such association between 25(OH) D and SVD in China. Methods: We retrospectively enrolled 106 patients with SVD and 115 controls between Jan 2017 and Dec 2017. All the subjects were categorized into three subgroups according to the level of 25(OH) D: vitamin D de�ciency (<12 ng/ml), insu�ciency (12-20 ng/ml) and su�ciency (>20 ng/ml). Results: Among 106 SVD patients, 80 (75.5%) were men and the mean age was 61.6±13.2 years. The de�ciency of 25(OH) D was observed in 76 (71.7%) of SVD patients and 47 (40.9%) of controls (P=0.001). Compared with controls, patients with SVD were more likely to be male, a stroke history, smokers, with hyperlipidemia, higher systolic and diastolic blood pressure and low-density lipoprotein, and lower of 25(OH)D level (P<0.05). Logistic regression analysis revealed the level of 25(OH)D as an independent predictor of SVD (OR 0.772, 95% CI 0.691-0.862, P=0.001). Compared with the su�cient 25(OH)D group, the ORs of SVD in de�cient and insu�cient 25(OH)D group were 5.609 (95% CI 2.006-15.683) and 1.077 (95% CI: 0.338-3.428) after adjusting for potential confounders, respectively. In hypertensives with vitamin D de�cient and insu�cient group compared with su�cient group, the ORs of SVD increased to 9.738 (95% CI 2.398-39.540) and 1.108 (95% CI 0.232-5.280), respectively (Pinteraction=0.001). Conclusion: We found signi�cant associations between SVD and 25(OH)D
Dietary phosphorus deficiency impaired growth , intestinal digestion and absorption function of 2 meat ducks 3 4

17 Objective: An experiment was conducted to investigate the effects of dietary non-phytate phosphorus 18 (nPP) deficiency on intestinal pH value, digestive enzyme activity, morphology, nutrient utilization, 19 and gene expression of NaPi-IIb in meat ducks from 1 to 21 d of age. 20 Methods: 525 one-d-old Cherry Valley ducklings were fed diets (with 7 pens of 15 ducklings, or 105 21 total ducklings, on each diet) with five levels of nPP (0.22, 0.34, 0.40, 0.46 or 0.58%) for 21 d in a 22 completely randomized design. Fiveexperimental diets contained a constant calcium (Ca) content of 23 approximately 0.9%. Body weight (BW), body weight gain (BWG), feed intake (FI), and feed to gain 24 ratio (F: G) were measured at 14 and 21 d of age. Ducks were sampled for duodenum and jejunum 25 digestion and absorption function on 14 and 21 d. Nutrient utilization was assessed using 25to 26 27-d-old ducks. 27 Results: The results showed ducks fed 0.22% nPP had lower (P<0.05) growth performance and 28 nutrient utilization and higher (P<0.05) serum Ca content andalkaline phosphatase (ALP) activity. 29 When dietary nPP levels were increased, BW(d 14 and 21), BWG and FI (all intervals), and the serum 30 phosphorus (P) content linearly and quadratically increased (P<0.05); and the jejunal pH value (d 14), 31 duodenal muscle layer thickness (d 14), excreta dry matter, crude protein, energy, Ca and total P 32 utilization linearly increased (P<0.05); however, the serum alkaline phosphatase (ALP) activity, jejunal 33 Na + -K + -ATPase activity, and duodenal NaPi-IIb mRNA level (d 21) linearly decreased (P<0.05). 34 Conclusion: The results indicated that ducks aged from 1 to 21d fed diets with 0.22% nPP had poor 35 growth performance related to poor intestinal digestion and absorption ability; but when fed diets with 36 0.40%, 0.46% and 0.58% nPP, ducks presented a better growth performance, intestinal digestion and 37 absorption function. 38 Ac ce pt ed A rti cle
Polyfunctional tumor-reactive T cells are effectively expanded from non-small cell lung cancers, and correlate with an immune-engaged T cell profile in situ

Non-small cell lung cancer (NSCLC) is the second most prevalent type of cancer. With the current treatment regimens, the mortality rate remains high. Therefore, better therapeutic approaches are necessary. NSCLCs generally possess many genetic mutations and are well infiltrated by T cells (TIL), making TIL therapy an attractive option. Here we show that T cells from treatment naive, stage I-IVa NSCLC tumors can effectively be isolated and expanded, with similar efficiency as from normal lung tissue. Importantly, 76% (13/17) of tested TIL products isolates from NSCLC lesions exhibited clear reactivity against primary tumor digests, with 0.5%-30% of T cells producing the inflammatory cytokine Interferon (IFN)-γ. Both CD4+ and CD8+ T cells displayed tumor reactivity. The cytokine production correlated well with CD137 and CD40L expression. Furthermore, almost half (7/17) of the TIL products contained polyfunctional T cells that produced Tumor Necrosis Factor (TNF)-α and/or IL-2 in addition to IFN-γ, a hallmark of effective immune responses. Tumor-reactivity in the TIL products correlated with high percentages of CD103+CD69+CD8+ T cell infiltrates in the tumor lesions, with PD-1hiCD4+ T cells, and with FoxP3+CD25+CD4+ regulatory T cell infiltrates, suggesting that the composition of T cell infiltrates may predict the level of tumor reactivity. In conclusion, the effective generation of tumor-reactive and polyfunctional TIL products implies that TIL therapy will be a successful treatment regimen for NSCLC patients.
Clinical Applications of Mesenchymal Stromal Cells (MSCs) in Orthopedic Diseases

Mesenchymal stromal cells (MSCs) have the capacity for self-renewal and multi-lineage dif- ferentiation, have many advantages over other cells, and are thought to be one of the most promising cell sources for cell-based treatments. In fact, MSCs have already been widely applied in clinics as a treatment for numerous disorders, including orthopedic diseases, such as bone fracture, articular cartilage injury, osteoarthritis (OA), femoral head necrosis, degenerative disc, meniscus injury, osteogenesis imperfecta (OI), and other systemic bone diseases. With the progressions in R&D, the safety and efficacy of MSC-based treatments in orthopedic diseases have been largely recognized, but many challenges still exist. In this chapter, we intend to briefly update the recent progressions and discuss the potential issues in the target areas. Hopefully, our discussion would be helpful not only for the clini-cians and the researchers in the specific disciplines but also for the general audiences.
Serveur Académique Lausannois SERVAL serval.unil.ch

Background— MYCN oncogene amplification has been defined as the most important prognostic factor for neuroblastoma, the most common solid extracranial neoplasm in children. High copy numbers are strongly associated with rapid tumor progression and poor outcome, independently of tumor stage or patient age, and this has become an important factor in treatment stratification. Procedure— By Real Time Quantitative PCR analysis, we evaluated the clinical relevance of circulating MYCN DNA of 267 patients with locoregional or metastatic neuroblastoma in children less than 18 months of age. Results— For patients in this age group with INSS stage 4 or 4S NB and stage 3 patients, serum-based determination of MYCN DNA sequences had good sensitivity (85%, 83% and 75% respectively) and high specificity (100%) when compared to direct tumor gene determination. In contrast, the approach showed low sensitivity patients with stage 1 and 2 disease. Conclusion— Our results show that the sensitivity of the serum-based MYCN DNA sequence determination depends on the stage of the disease. However, this simple, reproducible assay may represent a reasonably sensitive and very specific tool to assess tumor MYCN status in cases with stage 3 and metastatic disease for whom a wait and see strategy is often recommended.
aMPlIFY: a randomized, Phase III study evaluating the efficacy and safety of aclidinium/formoterol vs monocomponents and tiotropium in patients with moderate-to-very severe symptomatic COPD

and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). International Journal of COPD 2019:14 667–682 International Journal of COPD
Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET.

PURPOSE Our purpose was to establish an evaluation system for oocyte quality based on the incidence of cumulus cells apoptosis and to examine the effect of coculture, using autologous cumulus cells, on the outcome of IVF-ET according to proliferative activities of helper cells and the incidence of cumulus cells apoptosis. METHODS Cumulus cell masses were collected from 91 mature oocytes among 330 oocytes retrieved from a total of 34 IVF-ET cycles with tubal infertility and unexplained infertility. The incidence of apoptosis in cumulus cells was assessed by apoptosis detection kit fluorescein. On ovum pick up, 2nd day embryos were cocultured with autologous cumulus cells. Prior to coculture, in vitro proliferative activity of cumulus cells was evaluated. RESULTS Cumulus cells from patient groups over 40 years old had a significantly increased apoptosis incidence, a lower fertilization rate, and the decreased number of oocytes retrieved compared to the other age groups (P < .05). The incidence of cumulus cells apoptosis was significantly lower when the number of oocytes retrieved was 5 or less (P < .05). Cumulus cells from fertilized oocytes (0.43 +/- 0.07%) and those from patients who became pregnant (0.44 +/- 0.11%) following IVF-ET showed a significantly lower incidence of apoptosis compared to those of unfertilized oocytes (1.80 +/- 0.35%; P < .001) and the nonpregnant group (0.81 +/- 0.10%; P < .05). Embryo quality also had a negative correlation with the incidence of cumulus cells apoptosis. Coculture of fertilized oocytes with cumulus cells with high proliferative activity resulted in improved rates of implantation and pregnancy compared to that with poor active cumulus cells. No significant difference was found between the in vitro proliferative activity of cumulus cells and the incidence of cumulus cells apoptosis (P < .063). CONCLUSIONS The age of women might influence the incidence of apoptosis in cumulus cells, and the increased incidence of apoptosis is associated with the number of oocytes retrieved, the fertilization rate, and the pregnancy outcome following IVF-ET. These results suggest that the incidence of cumulus cells apoptosis can be used in predicting oocyte quality, outcome of IVF-ET, and age-related decline in fertility.