Miracle-dz/UniFAR-FTUs huggingface dataset:
Light-induced displacement of PLASTID MOVEMENT IMPAIRED1 precedes light-dependent chloroplast movements

Abstract Light-dependent chloroplast movements are an actin-dependent cellular response to changes in the light environment that help plants maximize photosynthetic potential and reduce photodamage. Over a dozen proteins are known to be required for normal chloroplast movements, but the molecular mechanisms regulating the transformation of light perception into chloroplast motility are not fully understood. Here, we show that in Arabidopsis (Arabidopsis thaliana) the actin-bundling plasma membrane-associated proteins THRUMIN1, PLASTID MOVEMENT IMPAIRED1 (PMI1), and KINESIN-LIKE PROTEIN FOR ACTIN-BASED CHLOROPLAST MOVEMENT1 (KAC1) interact through the 14-3-3 proteins KAPPA and OMEGA. We also show that the interaction of PMI1 with 14-3-3 KAPPA and OMEGA is regulated by blue light activation of the Phototropin2 photoreceptor. Live-cell confocal microscopy revealed light-induced dynamic changes in the cellular localizations of PMI1 and KAC1. In particular, PMI1 was relocated away from irradiated areas of the plasma membrane in less than a minute after blue light exposure, consistent with PMI1 playing a critical role in initiating light-dependent chloroplast movements. We present a modified conceptual model for high light-dependent chloroplast movements in which PMI1 acts as the mobile signal that initiates a coordinated sequence of changes in protein–protein and protein–plasma membrane interactions that initiate the chloroplast movement response and determine where in the cell chloroplasts are able to anchor to the plasma membrane.
Current understanding in deciphering trophoblast cell differentiation during human placentation

Abstract The placenta is a unique organ that forms during gestation and supports fetus survival and communication with the mother. However, of such an essential organ for a successful pregnancy, our knowledge is limited. New progress has been made for human placenta study in recent years. We herein summarize the current understanding of human placental trophoblast differentiation and the molecules that govern trophoblast cell lineage specification. More importantly, the powerful tools for placental studies are also described such as human trophoblast stem cells, 3-dimensional (3D) trophoblast organoids, engineering-based placental devices, and single-cell RNA sequencing. These advances have brought us new insights into placental development and provided multiple investigation strategies for deciphering molecular mechanisms.
A systematic data-driven approach to analyze sensor-level EEG connectivity: Identifying robust phase-synchronized network components using surface Laplacian with spectral-spatial PCA

Although conventional averaging across predefined frequency bands reduces the complexity of EEG functional connectivity (FC), it obscures the identification of resting-state brain networks (RSN) and impedes accurate estimation of FC reliability. Extending prior work, we combined scalp current source density (CSD; spherical spline surface Laplacian) and spectral-spatial PCA to identify FC components. Phase-based FC was estimated via debiased weighted phase-locking index from CSD-transformed resting EEGs (71 sensors, 8 min, eyes open/closed, 35 healthy adults, 1-week retest). Spectral PCA extracted 6 robust alpha and theta factors (86.6% variance). Subsequent spatial PCA for each spectral factor revealed seven robust regionally-focused (posterior, central, frontal) and long-range (posterior-anterior) alpha components (peaks at 8, 10 and 13 Hz) and a midfrontal theta (6 Hz) component, accounting for 37.0% of FC variance. These spatial FC components were consistent with well-known networks (e.g., default mode, visual, sensorimotor), and four were sensitive to eyes open/closed conditions. Most FC components had good-to-excellent internal consistency (odd/even epochs, eyes open/closed) and test-retest reliability (ICCs ≥ .8). Moreover, the FC component structure was generally present in subsamples (session × odd/even epoch, or smaller subgroups [n = 7-10]), as indicated by similarity of factor loadings across PCA solutions. Apart from systematically reducing FC dimensionality, our approach avoids arbitrary thresholds and allows quantification of meaningful and reliable network components that may prove to be of high relevance for basic and clinical research applications.
African American and Caucasian patients with Sézary syndrome have no differences in outcomes at an ethnically diverse urban medical center

Abstract Sézary syndrome (SS) is an aggressive cutaneous T-cell lymphoma with poor survival. We performed a retrospective review of SS patients at Emory University from 1990 to 2020. We collected data on race, clinical characteristics, therapy, and social determinants of health. Clinical endpoints were overall survival (OS) and time to next treatment (TTNT). Univariate association and multivariable analyses were assessed by Cox proportional hazards models. Among 62 patients, 45.2% were AA. The median OS and TTNT were 3.1 years and 6.3 months, respectively, with no difference by race. AA patients had a higher median baseline LDH (360 vs. 232, p = 0.002) and a longer delay in initiation of systemic therapy compared to CC patients (3.17 vs. 2.14 months, p = 0.039), but a shorter commute (<10 miles) and no difference in insurance coverage (p = 0.260). AA patients at an academic center had unique clinical features and treatment patterns, but similar survival to CC SS patients.
The quest for accountability of Health Facility Governing Committees implementing Direct Health Facility Financing in Tanzania: A supply-side experience

User committees, such as Health Facility Governing Committees, are popular platforms for representing communities and civil society in holding service providers accountable. Fiscal decentralization via various arrangements such as Direct Health Facility Financing is thought to strengthen Health Facility Governing Committees in improving accountability in carrying out the devolved tasks and mandates. The purpose of this study was to analyze the status of accountability of Health Facility Governing Committees in Tanzania under the Direct Health Facility Financing setting as perceived by the supply side. In 32 different health institutions, a cross-sectional design was used to collect both qualitative and quantitative data at one point in time. Data was collected through a closed-ended questionnaire, an in-depth interview, and a Focus Group Discussion. Descriptive statistics, multiple logistic regression, and theme analysis were used to analyze the data. According to the findings, Health Facility Governing Committees’ accountability is 78%. Committees have a high level of accountability in terms of encouraging the community to join community health funds (91.71%), receiving medicines and medical commodities (88.57%), and providing timely health services (84.29%). The health facility governance committee’s responsibility was shown to be substantially connected with the health planning component (p = 0.0048) and the financial management aspect (p = 0.0045). This study found that the fiscal decentralization setting permits Committees to be accountable for carrying out their obligations, resulting in improved health service delivery in developing nations.
The complex rostral morphology and the endoskeleton ossification process of two adult samples of Xiphias gladius (Xiphiidae)

Abstract The authors studied the morphology of the upper and lower jaws, vertebrae and dorsal‐fin rays of the teleost fish Xiphias gladius to analyse the skeletal architecture and ossification pattern. The analogies and differences among these segments were investigated to identify a common morphogenetic denominator of the bone tissue osteogenesis and modeling. The large fat glands in the proximal upper jaw and their relationship to the underlying cartilage (absent in the lower jaw) suggested that there is a mechanism that explains rostral overgrowth in the Xiphiidae and Istiophoriidae families. Thus far, the compact structure of the distal rostrum has been interpreted as being the result of remodeling. Nonetheless, no evidence of cutting cones, scalloped outer border of osteons and sequence of bright–dark bands in polarized light was observed in this study, suggesting a primary osteon texture formed by compacting of collagen matrix and mineral deposition in the fat stroma lacunae of the bone, but without being oriented in layers of the collagen fibrils. A similar histology also characterizes the circular structures present in the other examined segments of the skeleton. The early phases of fibrillogenesis carried out by fibroblast‐like cells occurred farther from the already‐calcified bone surface inside the fat stroma lacunae. The fibrillar matrix was compacted and underwent mineral deposition near the previously calcified bone surface. This pattern of collagen matrix synthesis and calcification was different from that of mammalian osteoblasts, especially concerning the ability to build a lacuno‐canalicular system among cells. Necrosis or apoptosis of the latter and refilling of the empty lacunae by mineral deposits might explain the anosteocytic bone formation.
The timing of Start is determined primarily by increased synthesis of the Cln3 activator rather than dilution of the Whi5 inhibitor

INTRODUCTION How cells convert shallow-input gradients into all-or-none decisions is a fundamental problem in biology. Commitment to cell division is thought to require a threshold level of cell growth, but how incremental changes in growth are measured by the cell is unknown. Various hypotheses have been proposed to explain growth-dependent cell cycle commitment in late G1 phase in budding yeast (called Start), including two recent models. One model (Schmoller et al., 2015) posits that as the nucleus grows during G1 phase, the nuclear concentration of the G1/S transcriptional inhibitor Whi5 slowly decreases, thereby increasing the probability of the Start transition. This model of passive dilution is predicated on estimates of Whi5 nuclear concentration as assessed by wide-field epifluorescence microscopy as a function of time in G1 phase daughter cells. An alternative model (Litsios et al., 2019) posits that an increase in protein synthesis toward the end of G1 rapidly drives up the concentration of the highly unstable G1 cyclin Cln3, which activates Cdc28 (Cdk1), thereby triggering the phosphorylation-mediated dissociation of Whi5 from the SBF G1/S transcription factor complex and activation of the G1/S program. This model of active control of Start is supported by measurements of protein synthetic rate and Cln3 concentration as a function of time. Notably, based on wide-field epifluorescence detection of a Whi5-fluorescent protein (FP) fusion and mass spectrometry, Litsios et al. (2019) concluded that there is only “a small or no change in Whi5 concentration” throughout G1, consistent with earlier reported results (Dorsey et al., 2018) using scanning Number and Brightness (sN&B) microscopy, a method that allows for determination of absolute protein concentrations. In their Letter to Molecular Biology of the Cell, Schmoller et al. (2022) raise questions about the results and conclusions presented in our published studies (Dorsey et al., 2018; Litsios et al., 2019). Here, we respond to the criticisms of Schmoller et al. and demonstrate how wide-field fluorescence microscopy experiments to determine nuclear Whi5 concentration dynamics can be confounded by uncontrolled effects, which include photobleaching, partial confocal effects, and nuclear-to-cytoplasmic volume scaling. Further, we provide additional experimental evidence demonstrating that nuclear Whi5 concentration is essentially constant as cells grow in G1 phase and that Cln3 and protein synthesis dynamics occur as reported in Litsios et al. (2019). These results suggest that instead of being triggered by dilution of the stable inhibitor Whi5, Start is rather primarily controlled by the increase in protein synthesis rate in G1 and the concomitant production of the unstable activator Cln3.
Probing the possibility of lesion formation/progression in vicinity of a primary atherosclerotic plaque: A fluid–solid interaction study and angiographic evidences

It is shown that certain locations in the arterial tree, such as coronary and cerebral arteries, are more prevalent to plaque formation. Endothelial activation and consequent plaque development are attributed to local hemodynamic parameters such as wall shear stress (WSS), oscillatory shear index (OSI), relative residence time (RRT), and stress phase angle. After a certain level of plaque progression, these hemodynamic parameters are disturbed before and after the plaque. In the current study, it is hypothesized that the vicinity of a primary lesion is susceptible for further degeneration and second plaque formation. A fluid–solid interaction (FSI) model of the coronary artery with different levels of asymmetric constriction, is simulated and the trend of hemodynamic parameters were studied in both of the plaque side (PS) and the opposite wall (facing the plaque [PF]). Also, a novel factor is introduced that can identify the high‐risk regions associated with WSS oscillations to negative values. Our results indicate that when more than half of the artery is constricted, the downstream of the plaque is highly exposed to endothelial pathogenesis the PS, such that negative WSS, and as well, critical values of OSI and RRT, that is, −1.2 Pa, 0.42 and 6.5 s, respectively arise in this region. PS endothelial cells in this region exposed to the highest risk of atherosclerosis based on the proposed index (3 out of 3). As well, three cases of angiographic images are provided that confirms existence of secondary lesion close to the primary one as predicted by our computational simulations.
Effects of Disability Severity on the Family Burden of Home-Dwelling Uygur and Kazakh Aged in Rural Western China: Family Function as a Mediator

Introduction: Caring for the disabled elderly puts heavy burdens on their families. This study aimed to explore family function (FF) as a mediator in the relationship between disability severity (DS) and family burden (FB) of the Uygur and Kazakh home-dwelling disabled elderly cared for by informal caregivers in China. Method: A cross-sectional design with multistage sampling was used to recruit 431 families with Uygur and Kazakh disabled elderly in Bortala, Xinjiang, China. Data were collected using the Activities of Daily Living Scale, Family Burden Interview Schedule, and Family Adaptation, Partnership, Growth, Affection, and Resolve Index Scale and then analyzed using hierarchical regression. Results: FF had a partial mediating effect on the relationship between DS and FB (β = 0.131; 95% confidence interval [CI] = [0.074, 0.197]; p < .01); the effective rate was 41.46%. Discussion: Multidisciplinary care teams should implement targeted interventions to improve FF of the disabled elderly to alleviate FB.
3D bioprinted GelMA/GO composite induces osteoblastic differentiation

Graft substitute is a mature treatment tool in craniofacial bone repair. However, stress shielding and immutability of structure limit its use in patients with congenital defects. Therefore, a regenerative graft would be best suited for repair. Mesenchymal stem cells (MSCs) have been shown to be feasible in regenerative medicine and the clinical treatment of bone repair. The aim of this study was to propose a strategy that would directly blend graphene oxide (GO) and MSCs with gelatin methacrylate anhydride (GelMA), as bioink, to generate the scaffold for bone regenerative repair. The survival and osteogenic capacity of MSCs in the composite bioink were assessed by cell viability and proliferation assays, along with expression analysis of osteogenesis-related genes and proteins, and targeted immunofluorescence. The introduction of GO to the printing process had no influence on cell printing, viability, or printability of GelMa. However, the GO-involved structure exhibited a positive influence on MSC proliferation, without significantly affecting cell viability. Alkaline phosphatase was expressed more in cells cultured with GO than in those with pure GelMA. In addition, GO promoted the expression of osteogenesis-related genes and proteins, such as osteopontin, osteocalcin, and RUNX2. Collectively, the composite bioink enhanced cell proliferation and adhesion, as well as osteogenic differentiation properties, compared with pure GelMA.
A Survey and Assessment of Culturally Based Education Programs for Native American Students in the United States

Afew years ago when I was conducting field hearings to discuss intergovernmental relations (state, federal, and tribal) in the education of American Indians/Alaska Natives connected to President Clinton’s Executive Order on American Indian and Alaska Native Education, a hearing was held in Arizona at Window Rock, capitol of the Navajo Nation. During a morning break from our discussions, I felt a tug upon my sleeve that beckoned me to turn around. At the other end of the tug was an older Navajo man who asked me if I wanted to know what the problem with Indian education was. I said, “Yes,
A Nearly Neutral Model of Molecular Signatures of Natural Selection after Change in Population Size

Abstract The nearly neutral theory is a common framework to describe natural selection at the molecular level. This theory emphasizes the importance of slightly deleterious mutations by recognizing their ability to segregate and eventually get fixed due to genetic drift in spite of the presence of purifying selection. As genetic drift is stronger in smaller than in larger populations, a correlation between population size and molecular measures of natural selection is expected within the nearly neutral theory. However, this hypothesis was originally formulated under equilibrium conditions. As most natural populations are not in equilibrium, testing the relationship empirically may lead to confounded outcomes. Demographic nonequilibria, for instance following a change in population size, are common scenarios that are expected to push the selection–drift relationship off equilibrium. By explicitly modeling the effects of a change in population size on allele frequency trajectories in the Poisson random field framework, we obtain analytical solutions of the nonstationary allele frequency spectrum. This enables us to derive exact results of measures of natural selection and effective population size in a demographic nonequilibrium. The study of their time-dependent relationship reveals a substantial deviation from the equilibrium selection–drift balance after a change in population size. Moreover, we show that the deviation is sensitive to the combination of different measures. These results therefore constitute relevant tools for empirical studies to choose suitable measures for investigating the selection–drift relationship in natural populations. Additionally, our new modeling approach extends existing population genetics theory and can serve as foundation for methodological developments.
Resilience and job satisfaction among out‐of‐hospital emergency medical service professionals: A cross‐sectional multi‐centric study

Abstract Aim We aim to describe the relationship between job satisfaction and compare levels of resilience among out‐of‐hospital emergency medical service professionals. Background The study of the impact of the working environment on health professionals has raised great interest. Job‐related variables and resilience can be a protective factor against stressful and demanding events at work. Methods A cross‐sectional survey comprising sociodemographic and job‐related variables was conducted among 406 workers (doctors, nurses, psychologists, and ambulance technicians) from the out‐of‐hospital emergency medical system in Spain. Resilience was self‐reported using the Connor‐Davidson Resilience Scale. Results Nursing professionals were less resilient compared with ambulance technicians (score difference 1.709, p = .008). As age increased, resilience was lower (r = −.118). Professionals with higher resilience scores were more satisfied in their work (OR = 1.06, 95% CI: 1.02–1.11), and professionals with higher psychological strength, gained from working with other colleagues, also showed greater job satisfaction (OR = 5.47, 95% CI: 2.55–11.73). Conclusion There was a positive association between resilience, job satisfaction and collaborative work. Professionals with greater psychological strength, gained from working with other colleagues, also showed higher levels of job satisfaction. Implications for Nursing Management Managers can use these results to influence the work environment to enhance job satisfaction and hence improve the resilience of the out‐of‐hospital emergency health care professionals.
Current and future strategies for radiation therapy in gastric cancer

Gastric cancers are heterogeneous. There are important differences between gastric and junctional tumors and a focus on evidence based on true gastric cancers is important to guide treatment decision and the design of innovative strategies. We present key evidence shaping the current use of radiotherapy, discuss technical radiotherapy considerations that are important to consider in multidisciplinary decision‐making, and highlight selected research strategies including radiotherapeutic advances, theranostic approaches and key ongoing clinical trials to watch that will shape the role of radiotherapy in gastric cancers for the future.
New insights and advances on pyomelanin production: from microbial synthesis to applications

Abstract Pyomelanin is a brown-black phenolic polymer and results from the oxidation of homogentisic acid (HGA) in the L-tyrosine pathway. As part of the research for natural and active ingredients issued from realistic bioprocesses, this work re-evaluates the HGA pigment and makes an updated inventory of its syntheses, microbial pathways, and properties, with tracks and recent advances for its large-scale production. The mechanism of the HGA polymerization is also well documented. In alkaptonuria, pyomelanin formation leads to connective tissue damage and arthritis, most probably due to the ROS issued from HGA oxidation. While UV radiation on human melanin may generate degradation products, pyomelanin is not photodegradable, is hyperthermostable, and has other properties better than L-Dopa melanin. This review aims to raise awareness about the potential of this pigment for various applications, not only for skin coloring and protection but also for other cells, materials, and as a promising (semi)conductor for bioelectronics and energy.