Miracle-dz/UniFAR-FTUs huggingface dataset:
Prevalence, Reasons, Predictors, Perceived Effects, and Regulation of Alcohol Use among Children in Ghana

Early initiation of alcohol consumption increases the risk of alcohol dependence and adverse health outcomes. Consequently, nations have enacted laws to make alcohol unlawful to be purchased by, sold to, or used in public by children. This study examined the lifetime prevalence of alcohol use among children and their reasons for consuming alcohol. In addition, the study investigated predictors of alcohol consumption and the effects of alcohol use on children. Finally, it examined the effectiveness of measures in place for regulating the sale and use of alcohol by children in the country. A total of 5024 children between the ages of 8 and 17 were sampled across the ten regions of Ghana using a cross-sectional convergent parallel mixed method. Children were interviewed with a semi-structured questionnaire, while focus group discussions were held with children, parents, and key informants. Lifetime prevalence of alcohol consumption was measured by “have you ever taken alcohol? ”. The study revealed that lifetime alcohol consumption was less prevalent (6.6%) among children. Sex, age, and region of residence were significant predictors of lifetime alcohol use among children. More than half of the children who reported ever taking alcohol were first introduced to drinking by friends, and more than six in ten children claimed having been intoxicated after drinking alcohol. The findings further revealed that efforts to control the sale and consumption of alcohol by children have proven difficult despite the existence of laws, policies, and national regulatory structures. While regulations on alcohol sales and consumption have been difficult to implement in rural areas, they have been successful in urban areas because institutions there ensure rigorous adherence to the regulations. The study encourages national organizations with responsibility for child protection and development to step up their regulation, investigation, and information-sharing efforts to discourage and limit children from purchasing and consuming alcohol.
High Daytime Temperature Responsive MicroRNA Profiles in Developing Grains of Rice Varieties with Contrasting Chalkiness

High temperature impairs starch biosynthesis in developing rice grains and thereby increases chalkiness, affecting the grain quality. Genome encoded microRNAs (miRNAs) fine-tune target transcript abundances in a spatio-temporal specific manner, and this mode of gene regulation is critical for a myriad of developmental processes as well as stress responses. However, the role of miRNAs in maintaining rice grain quality/chalkiness during high daytime temperature (HDT) stress is relatively unknown. To uncover the role of miRNAs in this process, we used five contrasting rice genotypes (low chalky lines Cyp, Ben, and KB and high chalky lines LaGrue and NB) and compared the miRNA profiles in the R6 stage caryopsis samples from plants subjected to prolonged HDT (from the onset of fertilization through R6 stage of caryopsis development). Our small RNA analysis has identified approximately 744 miRNAs that can be grouped into 291 families. Of these, 186 miRNAs belonging to 103 families are differentially regulated under HDT. Only two miRNAs, Osa-miR444f and Osa-miR1866-5p, were upregulated in all genotypes, implying that the regulations greatly varied between the genotypes. Furthermore, not even a single miRNA was commonly up/down regulated specifically in the three tolerant genotypes. However, three miRNAs (Osa-miR1866-3p, Osa-miR5150-3p and canH-miR9774a,b-3p) were commonly upregulated and onemiRNA (Osa-miR393b-5p) was commonly downregulated specifically in the sensitive genotypes (LaGrue and NB). These observations suggest that few similarities exist within the low chalky or high chalky genotypes, possibly due to high genetic variation. Among the five genotypes used, Cypress and LaGrue are genetically closely related, but exhibit contrasting chalkiness under HDT, and thus, a comparison between them is most relevant. This comparison revealed a general tendency for Cypress to display miRNA regulations that could decrease chalkiness under HDT compared with LaGrue. This study suggests that miRNAs could play an important role in maintaining grain quality in HDT-stressed rice.
Evolution of a Planar Wake in Adverse Pressure Gradient

In the interest of improving the predictability of high-lift systems at maximum lift conditions, a series of fundamental experiments were conducted to study the effects of adverse pressure gradient on a wake flow. Mean and fluctuating velocities were measured with a two-component laser-Doppler velocimeter. Data were obtained for several cases of adverse pressure gradient, producing flows ranging from no reversed flow to massively reversed flow. While the turbulent Reynolds stresses increase with increasing size of the reversed flow region, the gradient of Reynolds stress does not. Computations using various turbulence models were unable to reproduce the reversed flow.
INCIDENCE AND DETERMINANTS OF BULLYING IN PRIVATE DENTAL COLLEGES OF LAHORE

This study aimed to identify the incidence of bullying among dental students in Lahore and to find out the usual sources and forms of bullying. In three different private dental colleges of Lahore, a cross sectional survey of undergraduate dental students was conducted. Data was collected by using a standardized, pre-validated Negative Acts Questionnaire (NAQ-R) to identify tormenting of dental students for past 12 months from 150 students. One-Way ANOVA was applied to explore the difference of bullying prevalence among 3 colleges. Overall, 137 (91.3%) dental students reported bullying of various types, confronted over the past 12 months. Most commonly noticed bullying source in this study was trainee fellows. 94 (68.6%) of participants never lodged a complaint about bullying. Higher Percentage of Participants reported of being bullied in dental college which needs serious attention as well as action to control this situation.
Soluble CD93 lectin-like domain sequesters HMGB1 to ameliorate inflammatory diseases

Rationale: CD93, a C-type lectin-like transmembrane glycoprotein, can be shed in a soluble form (sCD93) upon inflammatory stimuli. sCD93 effectively enhances apoptotic cell clearance and has been proposed as an inflammatory disease biomarker. The function of sCD93 involved directly in inflammation remains to be determined. Herein, we attempted to examine the hypothesis that sCD93 might sequester proinflammatory high-mobility group box 1 protein (HMGB1), exerting anti-inflammatory properties. Methods: Different forms of soluble recombinant human CD93 (rCD93) were prepared by a mammalian protein expression system. rCD93-HMGB1 interaction was assessed using co-immunoprecipitation and solid-phase binding assays. Effects of soluble rCD93 were evaluated in HMGB1-induced macrophage and vascular smooth muscle cells (VSMC) activation and receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis, CaCl2-induced and angiotensin II-infused abdominal aortic aneurysm (AAA) formation and ovariectomized-induced osteoporosis in mice. Results: Protein binding studies revealed that soluble rCD93, via the lectin-like domain (D1), can bind to HMGB1 and intercept HMGB1-receptor interaction. Soluble rCD93 containing D1 inhibited HMGB1-induced proinflammatory cytokine production and intracellular mitogen-activated protein kinase (MAPK)/nuclear factor (NF)-κB activation in macrophages and VSMCs, thereby attenuating CaCl2-induced and angiotensin II-infused AAA models. During osteoclastogenesis, RANKL stimulated HMGB1 secretion that promoted RANKL-induced osteoclastogenesis in return. Soluble rCD93 containing D1 impeded RANKL-induced osteoclastogenic marker gene expression and intracellular MAPK/NF-κB signaling, thereby mitigating ovariectomized-induced osteoporosis. Conclusion: These findings demonstrate the therapeutic potential of soluble recombinant CD93 containing D1 in inflammatory diseases. Our study highlights a novel anti-inflammatory mechanism, i.e., sequestration of HMGB1, through which sCD93 prevents HMGB1-receptor interaction on effector cells and alleviates inflammation.
Onsager-molecule approach to screening potentials in strongly coupled plasmas

The solution of the exact integral equation for the liquid pair-structure in the asymptotic strong coupling limit for the plasma, as mapped on the Onsager charge-smearing optimization for the energy lower bound, features “Onsager atoms” and “Onsager molecules”. The universal properties of this asymptotic limit make it a natural reference starting point for an asymptotic strong coupling expansion for the fluid structure and thermodynamics, playing the role of an “ideal liquid” state. In particular, the leading strong coupling terms for the potential energy, direct correlation functions, and screening potentials for the Coulomb and Yukawa mixtures (corresponding to classical plasmas and electron screened classical plasmas), with full thermodynamic consistency, are presented. These are in complete agreement with the Alastuey-Jancovici analysis of early simulations data by Hansen in strong coupling, and with recent highly accurate simulations data of Ogata, Iyetomi, and Ichimaru. Data analysis errors lead Ogata, Iyetomi, and Ichimaru to incorrect results for the short range screening potentials in strong coupling. Their calculations for the short range screening potentials, bridge functions, and enhancement factors for nuclear reaction rates in strongly coupled plasmas, should be revised.
Impedance Spectroscopy-Based Detection of Viral RNA From Clinical Samples

Rapid detection of viruses is necessary for early diagnosis of the disease and initiation of treatment. However, currently popular quantitative polymerase chain reaction (qPCR) techniques involve multistep protocols which consume a lot of time and also use expensive optics for yielding accurate results. Electrochemical biosensors have developed into reliable analytical tools over the past decade for sensitive, specific, and rapid detection of many viruses. Electrochemical impedance spectroscopy (EIS) is being used more frequently in electrochemical sensors and biosensors for both characterizing the sensors throughout the various phases of sensor design, as well as a method for quantitative and qualitative measurements. In this study, a sensitive electronic detection scheme for viral RNA is developed based on nonfaradaic EIS. Impedance measurements corresponding to biomodification on the electrode surface is carried out through an in-house microchip. Sensitivity is included through gold nanoparticle labeling to the DNA primers and a dielectrophoretic (DEP) concentration step. All the tests are conducted with cDNA synthesized from Hepatitis C virus (HCV) RNA samples and the limit of detection (LOD) achieved through the currently developed chip is in the range of 30–35 ng/μL i.e., around 140 nM for a 327-bp cDNA molecule. Further, the device starts detection procedure within 15 min of initiation.
Achieving Sustainable Manufacturing and Green Organizations: Preliminary Research on Green Competencies

Sustainability manufacturing is crucial in many aspects in terms of environmental impact. It concerns the consumption of energy, raw materials and materials, as well as the emission of harmful substances and waste. The implementation of sustainability manufacturing requires many actions at various levels, including strategic, tactical and operational ones. In order to implement measures aimed at minimizing the negative impact of the company on the environment, employees’ competencies are needed. The article presents preliminary research on key green competencies for sustainability companies. The research was carried out in the form of individual interviews with medium and large production companies. The result of the research is the division of competencies (knowledge, skills and attitudes) into three stages of the organization’s development, indicating the key competencies for each stage of the development of sustainability management.
Ethno-religious Conflicts and the Challenges of National Security in Nigeria’s Fourth Republic

ABSTRACT The threats of ethnic, religious, and ethno-religious conflicts have endured in Nigeria and become increasingly alarming in the fourth republic, which started in 1999. Ethno-religious conflicts are hostile engagements where actors and their motivations reflect a combination of ethnic and religious interests. These conflicts are deeply rooted in colonial legacies, ethno-religious-based distributions of power and resources, and failure of the post-colonial state to advance unity in diversity and promote the welfare and security of all citizens without discrimination. Between 1999 and 2021, Nigeria recorded no fewer than 2811 incidences of ethnic conflict with 18,132 fatalities, 3703 incidences of religious conflict with 29,957 fatalities, and 117 incidences of ethno-religious conflict with 2420 fatalities. These conflicts have had negative consequences for the lives and properties of the affected population, political stability, national unity, integration, development, security, and the image of the country internationally. This article examines the threats of ethno-religious conflicts in Nigeria’s fourth republic, their causes, manifestations, consequences, and possible ways forward.
Delineating Zinc Influx Mechanisms during Platelet Activation

Zinc (Zn2+) is released by platelets during a hemostatic response to injury. Extracellular zinc ([Zn2+]o) initiates platelet activation following influx into the platelet cytosol. However, the mechanisms that permit Zn2+ influx are unknown. Fluctuations in intracellular zinc ([Zn2+]i) were measured in fluozin-3-loaded platelets using fluorometry and flow cytometry. Platelet activation was assessed using light transmission aggregometry. The detection of phosphoproteins was performed by Western blotting. [Zn2+]o influx and subsequent platelet activation were abrogated by blocking the sodium/calcium exchanged, TRP channels, and ZIP7. Cation store depletion regulated Zn2+ influx. [Zn2+]o stimulation resulted in the phosphorylation of PKC substates, MLC, and β3 integrin. Platelet activation via GPVI or Zn2+ resulted in ZIP7 phosphorylation in a casein kinase 2-dependent manner and initiated elevations of [Zn2+]i that were sensitive to the inhibition of Orai1, ZIP7, or IP3R-mediated pathways. These data indicate that platelets detect and respond to changes in [Zn2+]o via influx into the cytosol through TRP channels and the NCX exchanger. Platelet activation results in the externalization of ZIP7, which further regulates Zn2+ influx. Increases in [Zn2+]i contribute to the activation of cation-dependent enzymes. Sensitivity of Zn2+ influx to thapsigargin indicates a store-operated pathway that we term store-operated Zn2+ entry (SOZE). These mechanisms may affect platelet behavior during thrombosis and hemostasis.
Evaluating Brewers’ Spent Grain Protein Isolate Postprandial Amino Acid Uptake Kinetics: A Randomized, Cross-Over, Double-Blind Controlled Study

Valorization and utilization of brewers’ spent grain (BSG) are of great interest in terms of reducing food waste and promoting more sustainable food systems. In this study, we aimed to evaluate the nutritional value of upcycled barley/rice proteins (BRP) extracted from BSG and compare this with pea proteins (PP). A randomized, cross-over, double-blind controlled trial was conducted with twelve participants (age: 24 ± 2.8 years, BMI: 23.3 ± 3.0 kg/m2). During three separate visits with a one-week washout period between visits, participants received 20 g BRP, PP, or the benchmark protein whey (WP). Blood-free amino acids (AA) were measured to determine postprandial AA uptake kinetics. The estimated total AA (TAA) uptake of BRP was 69% when compared to WP and 87% when compared to PP. The time to reach the maximum values was similar between the three protein sources. When comparing individual essential AA responses between BRP and PP, we observed higher responses in methionine and tryptophane and lower responses in lysine, histidine, and isoleucine for BRP compared to PP. This study demonstrates that BRP exhibits comparable postprandial TAA uptake profiles to PP. The findings highlight the complementarity of BRP and PP, which may offer the potential for blending approaches to optimize protein quality for overall health.
Safety Profile of Vitamin D in Italy: An Analysis of Spontaneous Reports of Adverse Reactions Related to Drugs and Food Supplements

Vitamin D (VitD) is largely used in Italy, often inappropriately; thus, an evaluation of its safety is a crucial issue. This study analyses the adverse reactions (ARs) associated with the use of products containing VitD (VitDps) reported to the Italian National Pharmacovigilance and Phytovigilance networks. From March 2002 to August 2022, a total of 643 and 127 reports concerning 903 and 215 ARs were retrieved from Pharmacovigilance and Phytovigilance networks, respectively. Overall, 332 (29.6%) ARs were classified as serious, and the most described ones were hypercalcaemia, renal failure and tachycardia. Serious AR risk was significantly higher for subjects using more than four concomitant products (OR 2.44 [95% CI 1.30–4.60]) and VitD doses higher than 1000 IU/day (OR 2.70 [95% CI 1.30–5.64]). In Italy, there was a modest decrease in AR reporting, despite the slightly increased use of VitD during the COVID-19 pandemic. To the best of our knowledge, this is the first study describing all VitDps-related ARs observed in the Italian general population. Since underreporting is the main limitation of the safety reporting systems, the necessity to continue ARs monitoring, also using real-world data on VitDps prescription, use and outcome patterns is highlighted.
Diversity and potential of herbaceous plants as mercury (Hg) hyperaccumulators in small-scale gold mining sites in Pancurendang, Banyumas, Indonesia

Abstract. Muryani E, Sajidan, Budiastuti MTS, Pranoto. 2023. Diversity and potential of herbaceous plants as mercury (Hg) hyperaccumulators in small-scale gold mining sites in Pancurendang, Banyumas, Indonesia. Biodiversitas 24: 3378-3386. Plants resistant to mercury can be used as an alternative to processing gold mining waste with the phytoremediation method. The study aimed to inventory herbaceous plant species in artisanal and small-scale gold mining (ASGM) sites in Pancurendang Village (Banyumas, Central Java, Indonesia), measure mercury concentrations in plants and root zones, and analyze the potential of herbaceous plants as mercury (Hg) hyperaccumulators. Herbaceous plants were cataloged by purposive sampling with multiple quadrats of 2m x 2m in 14 locations and then analyzed to obtain density, frequency, dominance, importance value, and diversity index. Biomass and Hg concentrations were measured from selected herbaceous plants and soil samples of the root zone. The Biological Accumulation Coefficient (BAC) was calculated to determine the plants' potential as Hg hyperaccumulators. Plant inventory identified 54 herbaceous plant species belonging to 26 families, with Paspalum conjugatum P.J.Bergius being the dominant species. In the root zones, Hg was found at 11-73 ppm and 7.6-85.36 ppm in 19 species. Ipomea aquatica Forssk. accumulated the highest concentration (85.36 ppm) and absorbed the greatest amount of Hg (5.25 mg). Based on their BACs, the nineteen species were categorized into moderate to high (hyper) accumulators, with Plectranthus sp. having the highest BAC of 4.54.
Intelligent Energy-Aware Routing Protocol in Mobile IoT Networks Based on SDN

Intelligent devices and equipment have affected almost all aspects of our life and behavior. The type of connection and the manner of communication between this large volume of devices has caused the emergence of a vast field in the Internet called the Internet of Things, which significantly highlights the issue of energy management and increases the lifetime of networks. Complex communications, especially in mobile networks, have generated many challenges for network designers. To solve these challenges, the Software Defined Networking (SDN) paradigm has reduced the overhead in the devices caused by processing and computing by adding new capabilities to mobile IoT networks. This technique transfers energy-consuming tasks to the central controller, which manages continuous topological changes of the network in dynamic environments. This paper presents a new routing approach called Intelligent Energy-aware Routing Protocol in Mobile IoT Networks based on SDN (IERMIoT), which tries to manage the dynamic changes of topology due to the movement of mobile nodes to increase the network’s lifetime and prevent energy dissipation. For this purpose, it defines clusters of nodes and uses an intelligent evolutionary algorithm to determine the number of clusters required in the network and their balanced distribution in the dynamic environment. Also, this approach considers a mechanism to reduce the overhead of control packets and routing packets, which significantly affects the energy consumption of nodes. The simulation results indicate the proposed solution’s effectiveness compared to other simulated approaches with respect to packet delivery rate, average energy consumption, network lifetime, number of alive nodes, coverage, and routing overhead.
Dietary Supplementation with Popped Amaranth Modulates the Gut Microbiota in Low Height-for-Age Children: A Nonrandomized Pilot Trial

Amaranth has been recognized as a nutraceutical food because it contains high-quality proteins due to its adequate amino acid composition that covers the recommended requirements for children and adults. Since pre-Hispanic times, amaranth has been consumed as popped grain; the popping process improves its nutritive quality and improves its digestibility. Popped amaranth consumption has been associated with the recovery of malnourished children. However, there is no information on the impact that popped amaranth consumption has on gut microbiota composition. A non-randomized pilot trial was conducted to evaluate the changes in composition, structure, and function of the gut microbiota of stunted children who received four grams of popped amaranth daily for three months. Stool and serum were collected at the beginning and at the end of the trial. Short-chain fatty acids (SCFA) were quantified, and gut bacterial composition was analyzed by 16S rRNA gene sequencing. Biometry and hematology results showed that children had no pathology other than low height-for-age. A decrease in the relative abundance of Alistipes putredinis, Bacteroides coprocola, and Bacteroides stercoris bacteria related to inflammation and colitis, and an increase in the relative abundance of Akkermansia muciniphila and Streptococcus thermophiles bacteria associated with health and longevity, was observed. The results demonstrate that popped amaranth is a nutritious food that helps to combat childhood malnutrition through gut microbiota modulation.