Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical and microbiological characteristics of patients colonized or infected by Stenotrophomonas maltophilia: is resistance to sulfamethoxazole/trimethoprim a problem?

ABSTRACT Stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in the last decade. Increased resistance to sulfamethoxazole/trimethoprim (SMX/TMP) has been reported in S. maltophilia strains in the past few years, leading to few therapeutic options. We conducted a prospective multicenter study at two Brazilian teaching hospitals that identified S. maltophilia isolates and evaluated their antimicrobial susceptibility profile, SMX/TMP resistance genes and their clonality profile. A total of 106 non-repeated clinical samples of S. maltophilia were evaluated. Resistance to SMX/TMP was identified in 21.6% of the samples, and previous use of SMX/TMP occurred in 19 (82.6%). PCR detected the sul1 gene in 14 of 106 strains (13.2%). Of these isolates, nine displayed resistance to SMX/TMP. The resistant strains presented a polyclonal profile. This opportunistic pathogen has emerged in immunocompromised hosts, with few therapeutic options, which is aggravated by the description of emerging resistance mechanisms, although with a polyclonal distribution profile.
Highly Pathogenic Avian Influenza Clade 2.3.4.4b Subtype H5N8 Virus Isolated from Mandarin Duck in South Korea, 2020

In October 2020, a highly pathogenic avian influenza (HPAI) subtype H5N8 virus was identified from a fecal sample of a wild mandarin duck (Aix galericulata) in South Korea. We sequenced all eight genome segments of the virus, designated as A/Mandarin duck/Korea/K20-551-4/2020(H5N8), and conducted genetic characterization and comparative phylogenetic analysis to track its origin. Genome sequencing and phylogenetic analysis show that the hemagglutinin gene belongs to H5 clade 2.3. 4.4 subgroup B. All genes share high levels of nucleotide identity with H5N8 HPAI viruses identified from Europe during early 2020. Enhanced active surveillance in wild and domestic birds is needed to monitor the introduction and spread of HPAI via wild birds and to inform the design of improved prevention and control strategies.
18F-FDG PET/CT for Target Volume Contouring in Lung Cancer Radiotherapy.

In 2005, Nestle et al. ([1][1]) published their seminal paper comparing different methods to delineate radiotherapy target volume from 18F-FDG PET in non–small cell lung cancer (NSCLC). They compared 4 different methods for primary delineation of gross-tumor target volume: a visual method, 2 fixed
Validity and reliability of the Danish version of the pregnancy physical activity questionnaire to assess levels of physical activity during pregnancy

Abstract Introduction Physical inactivity during pregnancy is associated with complications on the mother and child. Thus, assessments of physical activity in pregnant women is relevant. The American validated questionnaire, Pregnancy Physical Activity Questionnaire (PPAQ), measures physical activity during pregnancy, and a translated Danish version of the questionnaire is needed. Objectives To translate the original version of PPAQ into Danish (PPAQ-DK) and to determine face and content validity, and reliability of PPAQ-DK in pregnant Danish women. Materials and methods PPAQ was translated into Danish through a systematic process. Subsequently, face and content validity was examined on pregnant women. PPAQ-DK was revised, and the test-retest reliability of PPAQ-DK was investigated in another sample of pregnant women, where the respondents completed the questionnaire twice with one week apart, using an Intraclass Correlation Coefficient (ICC). Internal consistency reliability was determined using Cronbach´s α. Results The face validity of the original PPAQ was achieved by interviews of 10 pregnant women (age (mean ± SD): 31.0 ± 3.0 years; 50% in second and 50% in third trimester) and minor changes in the revised version of PPAQ-DK were performed. The content validity was accepted by an expert panel. In 66 pregnant women (aged 30.2 ± 4.8 years; 16.7, 63.6, and 19.7% in first, second and third trimester, respectively), the ICC in the test-retest reliability was 0.79 (0.68–0.87) of the overall PPAQ score. The Cronbach's α in the internal consistency reliability analysis was 0.7 in the overall PPAQ score. Conclusions The PPAQ-DK is within acceptable reliability and is a valid and reliable tool to measure overall physical activity level of pregnant Danish women.
Bactericides Based on Copper Nanoparticles Restrain Growth of Important Plant Pathogens

Copper nanoparticles (CuNPs) can offer an alternative to conventional copper bactericides and possibly slow down the development of bacterial resistance. This will consequently lower the accumulation rate of copper to soil and water and lower the environmental and health burden imposed by copper application. Physical and chemical methods have been reported to synthesize CuNPs but their use as bactericides in plants has been understudied. In this study, two different CuNPs products have been developed, CuNP1 and CuNP2 in two respective concentrations (1500 ppm or 300 ppm). Both products were characterized using Dynamic Light Scattering, Transmission Electron Microscopy, Attenuated Total Reflection measurements, X-ray Photoelectron Spectroscopy, X-ray Diffraction and Scattering, and Laser Doppler Electrophoresis. They were evaluated for their antibacterial efficacy in vitro against the gram-negative species Agrobacterium tumefaciens, Dickeya dadantii, Erwinia amylovora, Pectobacterium carotovorum, Pseudomonas corrugata, Pseudomonas savastanoi pv. savastanoi, and Xanthomonas campestris pv. campestris. Evaluation was based on comparisons with two commercial bactericides: Kocide (copper hydroxide) and Nordox (copper oxide). CuNP1 inhibited the growth of five species, restrained the growth of P. corrugata, and had no effect in X. c. pv campestris. MICs were significantly lower than those of the commercial formulations. CuNP2 inhibited the growth of E. amylovora and restrained growth of P. s. pv. savastanoi. Again, its overall activity was higher compared to commercial formulations. An extensive in vitro evaluation of CuNPs that show higher potential compared to their conventional counterpart is reported for the first time and suggests that synthesis of stable CuNPs can lead to the development of low-cost sustainable commercial products.
The end of AIDS? HIV and the new landscape of illness in rural South Africa

ABSTRACT The global HIV/AIDS scientific community has begun to hail the dawn of ‘the End of AIDS’ with widespread anti-retroviral therapy (ART) and dramatic declines in AIDS-related mortality. Drawing on community focus groups and in-depth individual interviews conducted in rural South Africa, we examine the complex unfolding of the end of AIDS in a hard-hit setting. We find that while widespread ART has led to declines in AIDS-related deaths, stigma persists and is now freshly motivated. We argue that the shifting landscape of illness in the community has produced a new interpretive lens through which to view living with HIV and dying from AIDS. Most adults have one or more chronic illnesses, and ART-managed HIV is now considered a preferred diagnosis because it is seen as easier to manage, more responsive to medication, and less dangerous compared to diseases like cancer, hypertension, and diabetes. Viewed through this comparative lens, dying from AIDS elicits stigmatising individual blame. We find that blame persists despite community acknowledgement of structural barriers to ART adherence. Setting the ending of AIDS within its wider health context sheds light on the complexities of the epidemiological and health transitions underway in much of the developing world.
Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors

Sporisorium scitamineum is a biotrophic fungus causing sugarcane smut disease. In this study, we set up a pipeline and used genomic and dual transcriptomic data previously obtained by our group to identify candidate effectors of S. scitamineum and their expression profiles in infected smut-resistant and susceptible sugarcane plants. The expression profile of different genes after infection in contrasting sugarcane genotypes assessed by RT-qPCR depended on the plant genotypes and disease progression. Three candidate effector genes expressed earlier only in resistant plants, four expressed in both genotypes, and three later in susceptible plants. Ten genes were cloned and transiently expressed in N. benthamiana leaves to determine their subcellular location, while four localized in more than one compartment. Two candidates, g3890 having a nucleoplasmic and mitochondrial location and g5159 targeting the plant cell wall, were selected to obtain their possible corresponding host targets using co-immunoprecipitation (CoIP) experiments and mass spectrometry. Various potential interactors were identified, including subunits of the protein phosphatase 2A and an endochitinase. We investigated the presence of orthologs in sugarcane and using transcriptome data present their expression profiles. Orthologs of sugarcane shared around 70% similarity. Identifying a set of putative fungal effectors and their plant targets provides a valuable resource for functional characterization of the molecular events leading to smut resistance in sugarcane plants and uncovers further opportunities for investigation.
Enter the Matrix: Use of Secondary Matrix by Mouse Lemurs

Madagascar is home to many threatened and endemic primate species, yet this island has seen dramatic declines in lemur habitat due to forest loss. This forest loss has resulted in an increasingly fragmented forest landscape, with fragments isolated from each other by grasslands (i.e., matrix). The grassland matrix is not entirely homogeneous containing matrix elements such as isolated trees and shrubs and linear features such as drainage lines. Because most lemurs are predominantly arboreal, they may preferentially use matrix elements to facilitate dispersal between fragments for access to mates or reduce feeding competition, allowing gene flow between fragments of habitat. Therefore, it is important to understand to what degree they use the matrix. We investigated matrix use in two mouse lemurs, the grey mouse lemur (Microcebus murinus) and the golden-brown mouse lemur (Microcebus ravelobensis) in a fragmented landscape in northwest Madagascar. We tested the predictions that: (1) lemurs use matrix less often than forest fragments, (2) if they use the matrix, then they will preferentially use matrix elements compared to grassland, and (3) M. murinus will disperse into the matrix further than M. ravelobensis. In 2011, we visually surveyed line transects in four areas containing matrix elements and four adjacent forest fragments during nocturnal walks. In 2017, we set up traplines in four areas of the matrix containing matrix elements, three areas that were grassland, and six traplines in adjacent fragments. We compared the relative abundance of mouse lemurs in matrix transects to fragmented forest transects, and the relative abundance of captured lemurs in matrix elements, grassland, and fragment traplines. We found that encounter rates of mouse lemurs did not significantly differ between the matrix and fragmented forest transects or traplines. Our sample size was too low to determine if the mean distance from the forest was greater for either Microcebus spp. Our study highlights that mouse lemurs do use matrix elements and there may be interspecific differences in use. Further research is needed to confirm species-specific matrix use, why mouse lemurs use matrix, and how much matrix elements facilitate movement for each species in fragmented landscapes.
Subcritical Water Extraction of Phenolic Compounds from Onion Skin Wastes (Allium cepa cv. Horcal): Effect of Temperature and Solvent Properties

The valorization of onion skin wastes (OSW) through the extraction, identification, and quantification of phenolic compounds was studied in this work, using subcritical water in a semicontinuous extractor (2.5 mL/min; 105–180 °C; 5 MPa). The extraction of flavonoids resulted to be fast (<30 min) and temperature sensitive (maximum at 145 °C; total flavonoids, 27.4 ± 0.9 mg/g dry OSW (DOSW)). The experimental results were fitted to the Weibull model. The influence of the solvent properties on the flavonoids quantification was found to be critical. A precipitate was formed once the extracts cooled down. If removed, a significant fraction of the high temperature extracted flavonoids (as much as 71%, at 180 °C) was lost. Such a condition affected especially those compounds that show extremely low solubility in water at room temperature, whereas quercetin glycosylated derivatives were less affected by the polarity change of the medium induced by the temperature change. It was demonstrated that it is necessary to re-dissolve the subcritical water extracts by the addition of ethanol, which led to a medium with a polarity equivalent to that obtained with water at high temperature. At 145 °C, quercetin (15.4 ± 0.4 mg/g DOSW) and quercetin-4′-glucoside (8.4 ± 0.1 mg/g DOSW) accounted for the 90% of the total flavonoids identified. By recovering high added value bioactive compounds from OSW the principles of circular economy were fulfilled, providing a new use for this agricultural waste.
The blood-spinal cord barrier in spinal cord injury: a review.

The blood-spinal cord barrier (BSCB), a physical barrier between the blood and spinal cord parenchyma, prevents the toxins, blood cells, and pathogens from entering the spinal cord and maintains a tightly controlled chemical balance in the spinal environment, which is necessary for proper neural function. However, BSCB disruption plays an important role in primary and secondary injury processes related to spinal cord injury (SCI). Following SCI, the structure of the BSCB is broken down, which directly leads to leakage of blood components. At the same time, the permeability of the BSCB is also increased. Repairing the disruption of the BSCB could alleviate the SCI pathology. Here, we review the morphology and pathology of the BSCB and progression of therapeutic methods targeting BSCB in SCI.
Underwater Local Cavity Welding of S460N Steel

In this paper, a comparison of the mechanical properties of high-strength low-alloy S460N steel welded joints is presented. The welded joints were made by the gas metal arc welding (GMAW) process in the air environment and water, by the local cavity welding method. Welded joints were tested following the EN ISO 15614-1:2017 standard. After welding, the non-destructive—visual, penetrant, radiographic, and ultrasonic (phased array) tests were performed. In the next step, the destructive tests, as static tensile-, bending-, impact- metallographic (macroscopic and microscopic) tests, and Vickers HV10 measurements were made. The influence of weld porosity on the mechanical properties of the tested joints was also assessed. The performed tests showed that the tensile strength of the joints manufactured in water (567 MPa) could be similar to the air welded joint (570 MPa). The standard deviations from the measurements were—47 MPa in water and 33 MPa in the air. However, it was also stated that in the case of a complex state of stress, for example, bending, torsional and tensile stresses, the welding imperfections (e.g., pores) significantly decrease the properties of the welded joint. In areas characterized by porosity the tensile strength decreased to 503 MPa. Significant differences were observed for bending tests. During the bending of the underwater welded joint, a smaller bending angle broke the specimen than was the case during the air welded joint bending. Also, the toughness and hardness of joints obtained in both environments were different. The minimum toughness for specimens welded in water was 49 J (in the area characterized by high porosity) and in the air it was 125 J (with a standard deviation of 23 J). The hardness in the heat-affected zone (HAZ) for the underwater joint in the non-tempered area was above 400 HV10 (with a standard deviation of 37 HV10) and for the air joint below 300 HV10 (with a standard deviation of 17 HV10). The performed investigations showed the behavior of S460N steel, which is characterized by a high value of carbon equivalent (CeIIW) 0.464%, during local cavity welding.
Factors associated with mental health outcomes among health care workers in the Fangcang shelter hospital in China

Background: Faced with surging infections and considering that 80% of cases were mild and moderate, the city government transformed stadiums and exhibition centers into the Fangcang shelter hospitals (temporary treatment centers) in Wuhan. Aim: The aims of this study were to evaluate psychological impact on health care workers in the Fangcang shelter hospitals and analyze potential risk factors associated with these symptoms, including PTSS, symptoms of anxiety, depression, insomnia, and perceived stress. Methods: This cross-sectional study used an online survey to assess mental health problems of 522 health care workers in a Jianghan Fangcang shelter from February 21 to February 28, 2020. Results: Overall, 25.3%, 25.3%, 51.0%, 58.0%, 14.8%, and 39.1% of all participants reported symptoms of posttraumatic stress, anxiety, depression, insomnia, and perceived stress, respectively. Multivariable logistic regression analysis showed that, occupation and technical title were associated with all investigated outcomes. Nurses were more likely to endorse posttraumatic stress symptoms (OR, 4.63; 95% CI, 2.43–8.82; p < .001), anxiety symptom (OR, 4.58; 95% CI, 2.76–7.62; p < .001), depression symptom (OR, 3.45; 95% CI, 2.17–5.64; p < .001). Participants with senior technical title were associated with higher perceived stress and insomnia than other technical title. Conclusion: In this survey, participants reported high rates of symptoms of posttraumatic stress, anxiety, depression, insomnia, and perceived stress. Combining with the special environment of the shelter hospitals and the psychological needs, the health care workers should be given more effective interventions to relieve their stress and improve mental symptoms.
A meta-analysis of the problematic social media use and mental health

Background: Although previous meta-analyses were conducted to quantitatively synthesize the relation between problematic social media (SM) use and mental health, they focused on Facebook addiction. Aims: The purpose of this meta-analysis is to examine this relation by extending the research scope via the inclusion of studies examining problematic use of all platforms. Method: One hundred and thirty-three independent samples (N =244,676) were identified. Results: As expected, the mean correlations between problematic SM use and well-being are negative, while those between problematic SM use and distress are positive. Life satisfaction and self-esteem are commonly used to represent well-being, while depression and loneliness are usually used to indicate distress. The mean correlations of problematic SM use with life satisfaction and self-esteem are small, whereas those of problematic SM use with depression and loneliness are moderate. The moderating effects of publication status, instruments, platforms and mean age are not significant. Conclusions: The magnitude of the correlations between problematic SM use and mental health indicators can generalize across most moderator conditions.
Evaluation of Suboptimal Peak Inspiratory Flow in Patients with Stable COPD

Objective: Although the importance of assessing inspiratory flow in the selection of treatments for chronic obstructive pulmonary disease (COPD) is understood, evaluation of this factor is not yet widespread or standardized. The objective of the present work was to evaluate the peak inspiratory flow (PIF) of patients with COPD and to explore the variables associated with a suboptimal PIF. Methods: An observational, cross-sectional study was carried out at specialized nursing consultations over a period of 6 months. We collected clinical data as well as data on symptoms, treatment adherence, and patient satisfaction with their inhalers via questionnaires. PIF was determined using the In-Check Dial G16® device (Clement Clarke International, Ltd., Harlow, UK). In each case, the PIF was considered suboptimal when it was off-target for any of the prescribed inhalers. The association with suboptimal PIF was evaluated using multivariate logistic regression and the results were expressed as the odds ratio (OR) with 95% confidence interval (CI). Results: A total of 122 COPD patients were included in this study, of whom 34 (27.9%) had suboptimal PIF. A total of 229 inhalers were tested, of which 186 (81.2%) were dry powder devices. The multivariate analysis found an association between suboptimal PIF and age (OR = 1.072; 95% CI (1.019, 1.128); p = 0.007) and forced vital capacity (OR = 0.961; 95% CI (0.933, 0.989); p = 0.006). Conclusions: About a third of patients in complex specialized COPD care have suboptimal PIFs, which is related to age and forced vital capacity.
Assessment of Tribological Properties of Ti3C2 as a Water-Based Lubricant Additive

Water-based lubrication has attracted remarkable interest due to its environmental and economic advantages. However, practical applications of water-based lubrication are often limited, mainly because of low viscosity and corrosivity. The use of additives has been proposed to overcome these limitations. In this work, the tribological characteristics of titanium carbide (Ti3C2) MXenes, as additives for water-based lubrication, were systematically investigated for contact sliding between stainless steel under various normal forces and Ti3C2 concentrations. Both friction and wear were found to decrease with increasing Ti3C2 concentration up to 5 wt%, and then increased when the concentration was larger than 5 wt%. The results suggest that Ti3C2 flakes hindered direct contact, particularly at the edges of the contact interfaces. It was further shown that the agglomeration of Ti3C2 flakes may have reduced the hindering when an excessive amount of Ti3C2 (e.g., 7 wt%) was applied. The decreases in the friction coefficient and wear rate with 5 wt% of Ti3C2 concentration w approximately 20% and 48%, respectively. The outcomes of this work may be helpful in gaining a better understanding of the tribological properties of Ti3C2 as a feasible water-based lubrication additive.
Highly Multifunctional GNP/Epoxy Nanocomposites: From Strain-Sensing to Joule Heating Applications

A performance mapping of GNP/epoxy composites was developed according to their electromechanical and electrothermal properties for applications as strain sensors and Joule heaters. To achieve this purpose, a deep theoretical and experimental study of the thermal and electrical conductivity of nanocomposites has been carried out, determining the influence of both nanofiller content and sonication time. Concerning dispersion procedure, at lower contents, higher sonication times induce a decrease of thermal and electrical conductivity due to a more prevalent GNP breakage effect. However, at higher GNP contents, sonication time implies an enhancement of both electrical and thermal properties due to a prevalence of exfoliating mechanisms. Strain monitoring tests indicate that electrical sensitivity increases in an opposite way than electrical conductivity, due to a higher prevalence of tunneling mechanisms, with the 5 wt. % specimens being those with the best results. Moreover, Joule heating tests showed the dominant role of electrical mechanisms on the effectiveness of resistive heating, with the 8 wt. % GNP samples being those with the best capabilities. By taking the different functionalities into account, it can be concluded that 5 wt. % samples with 1 h sonication time are the most balanced for electrothermal applications, as shown in a radar chart.