Miracle-dz/UniFAR-FTUs huggingface dataset:
Validation of LAP1B as a novel protein phosphatase 1 regulator

Protein phosphatase 1 (PP1) is a ubiquitously expressed serine/threonine protein phosphatase, with high expression levels in brain. PP1 regulates many cellular processes through interaction with different regulatory subunits that play regulatory and targeting roles. From yeast two-hybrid screens of a human brain cDNA library using PP1 isoforms as bait, lamina associated polypeptide 1B (LAP1B) was identified as a novel putative PP1 regulator. The functional role of LAP1B is poorly understood, but it is known that LAP1B is an inner nuclear membrane protein that interacts with lamins and torsinA, a protein involved in early onset torsion dystonia (EOTD). Furthermore, LAP1B has three potential PP1 binding motifs, a second generic PP1 binding motif named SILK and several phosphorylation sites. Thereby, we confirmed the PP1/LAP1B interaction by a variety of techniques. By yeast co-transformation we confirmed the interaction of LAP1B with PP1 isoforms alpha (α), gamma1 (γ1) and gamma2 (γ2) and demonstrated that LAP1B interacts with PP1γ1 through binding motif 1 (BM1, REVRF) that is located in the nucleoplasmic portion of LAP1B. The PP1/LAP1B interaction through the nucleoplasmic portion of LAP1B was also confirmed in vitro by blot overlay assay with purified PP1γ1 protein. The PP1/LAP1B interaction in vivo was evaluated by coimmunoprecipitation with specific antibodies and by co-localization between LAP1B and PP1α and PP1γ, ocurring at subnuclear structures and at locations near the nuclear envelope. The functional role of PP1/LAP1B complex was not determinated but it may be important in the maintenance of the nuclear architecture during mitosis. Given the high expression levels of PP1 within striatum and the relationship between basal ganglia dopaminergic signalling and EOTD, the existence of a putative tricomplex PP1/LAP1B/torsinA and the hypothesis that PP1 and/or abnormal protein phosphorylation may have a role in EOTD should be worthwhile exploring. Validation of LAP1B as a novel PP1 regulator Mestrado em Biologia Molecular e Celular 7 Abbreviations . 9
Growth and Reproduction of Atlantic Chub Mackerel ( Scomber colias ) in the Northwest Atlantic

ii ACKNOWLEDGMENTS . iii LIST OF TABLES . v LIST OF ILLUSTRATIONS . vi CHAPTER I – AGE AND GROWTH OF ATLANTIC CHUB MACKEREL IN THE NORTHWEST ATLANTIC . 1 1.
Susceptibility of Some Clinically Resistant Bacterial Isolates to Thyme Essential Oil, Chitosan and Lactobacillus reuteri

to piperacillin, co-amoxiclav, cephalothin, ceftazidime and imipenem. Meanwhile, 83% of S. aureus isolates were of the MRSA phenotype. The tested isolates displayed varying degrees of susceptibilities to the tested natural agents where thyme essential oil exhibited the highest inhibitory effect followed by chitosan and the culture supernatant of L. reuteri respectively . Albeit the high resistance displayed by both E. coli and Klebsiella isolates, they were the most susceptible to thyme essential oil. Contrariwise, L. reuteri exhibited the highest inhibitory effect against S. aureus isolates. Intriguingly, there was a general tendency for higher effectiveness of the natural products tested against the most resistant isolates implying that these natural products may have a resistance modifying potential. The presently investigated natural agents hold a promising potential against clinically significant multidrug resistant bacteria.
A quantitative inventory of yeast P body proteins reveals principles of compositional specificity

P bodies are archetypal biomolecular condensates that concentrate proteins and RNA without a surrounding membrane. While dozens of P body proteins are known, the concentrations of components in the compartment have not been measured. We used live cell imaging to generate a quantitative inventory of the major proteins in yeast P bodies. Only 7 proteins are highly concentrated in P bodies (5.1-15 μM); the 24 others examined are appreciably lower (most ≤ 2.4 μM). P body concentration correlates inversely with cytoplasmic exchange rate. Sequence elements driving Dcp2 concentration into P bodies are distributed across the protein and act cooperatively. Strong correlation between interaction valency of components and P body concentration suggests how the composition of other condensates may be estimated from known interaction data. Our data indicate that P bodies, and probably other condensates, are biochemically simpler than suggested by proteomic analyses, with implications for specificity, reconstitution and evolution.
Effects of Physical Activity and Counselling Interventions on Health Outcomes among Working Women in Shanghai: A randomized controlled trial

Background：Working women in Shanghai are a special group with a high risk of suffering work stress and burnout. They were disturbed by the work-family conflicts, which resulted in lower health-related quality of life (HRQoL), higher job stress and burnout. This study evaluated the potential physical activity and counselling intervention effects on health outcomes of working women in Shanghai participating in a group- or an individual-based physical activity and counselling intervention compared to a control group.Methods：Participants were randomly recruited from eight communities of Shanghai using the stratified cluster sampling method. A sample of 121 female workers was used in this study. They were randomly divided into three groups: a control group and two intervention groups (individual-based and group-based intervention). The two intervention groups accepted 12-week moderate physical activity and counselling intervention. Subjective perceptions of work stress, burnout, and HRQoL were measured before and after the intervention. Results：In the control group, no significant difference was found between participants for stress (p = 0.752) and burnout (p = 0.622) before and after the experiment. However, the HRQoL value decreased after the experiment, with the mean value falling from 91.59 to 87.10. After the experiment, the values of stress and burnout decreased, and the value of HRQoL increased in the two intervention groups. At the intervention’s completion, there were significant differences compared the two intervention groups to the control group separately regarding changes in burnout and HRQoL (all p < 0.01). For stress, the group-based intervention group exhibited a significant difference compared to the control group (p < 0.01), while the individual-based intervention group did not exhibit a significant difference compared to the control group (p = 0.128). Conclusion: Physical activity and counselling intervention delivered either in a group or individual format could reduce burnout and improve HRQoL of working women in Shanghai, and the group interventions were more effective than those targeted at individuals.
Influence of process parameters on quality of copper in electron-beam melting

Results are presented and discussed obtained by experimental and theoretical investigation concerning the influence of process parameters (melting power and refining time) on the quality of copper metal ingots in electron-beam melting. The thermodynamic and kinetic conditions are analyzed. Possible refining process mechanisms under different technological conditions are investigated. Appropriate processing regimes are proposed for efficient refining of copper and for obtaining high-quality metal after e-beam melting.
Winter hardiness of Miscanthus (II): Genetic mapping for overwintering ability and adaptation traits in three interconnected Miscanthus populations

Miscanthus × giganteus (M×g) is the primary species of Miscanthus for bioenergy feedstock production. The current leading biomass cultivar, M×g ‘1993‐1780’, is insufficiently adapted in temperate regions with cold winters such as USDA hardiness zone 5 (average annual minimum temperature of −28.9 to −23.3°C) or lower. Three interconnected Miscanthus F1 populations that shared a common parent were planted in a replicated field trial at Urbana, IL (hardiness zone 5b; average annual minimum temperature of −26.1 to −23.3°C) in spring 2011. The winter of 2013–2014 was especially cold in Urbana, with a minimum soil temperature at 10 cm of −6.2°C and a minimum air temperature of −25.3°C, giving us an opportunity to evaluate hardiness on established year‐3 plants. The parent in common to all three populations, M. sinensis ssp. condensatus ‘Cosmopolitan’, is native to maritime southern Japan, and in Urbana, it is winter‐damaged most years. In contrast, the three other parents, M. sacchariflorus ‘Robustus’ (MapA), M. sinensis ‘Silberturm’ (MapB), and M. sinensis ‘November Sunset’ (MapC), are typically winter hardy in Urbana. Nearly all MapA progeny plants survived and grew vigorously in spring 2014, whereas in MapB and MapC, many progeny plants did not survive the winter, and most of the survivors were severely damaged, with poor vigor. Negative correlations between overwintering ability and spring regrowth date and autumn dormancy date suggested that the genotypes most likely to survive winters were those that emerged early in spring and/or went dormant early in autumn. Using joint‐population analysis, we identified 53 quantitative trait loci (QTLs) for nine adaptation traits, including nine QTLs for overwintering ability and 11 for spring hardiness scores. Many biologically intuitive candidate genes were observed within or near the QTLs detected in this study, suggesting their validity and potential for further study.
Drug discovery and formulation development for acute pancreatitis

Abstract Acute pancreatitis is a sudden inflammation and only last for a short time, but might lead to a life-threatening emergency. Traditional drug therapy is an essential supportive method for acute pancreatitis treatment, yet, failed to achieve satisfactory therapeutic outcomes. To date, it is still challenging to develop therapeutic medicine to redress the intricate microenvironment promptly in the inflamed pancreas, and more importantly, avoid multi-organ failure. The understanding of the acute pancreatitis, including the causes, mechanism, and severity judgment, could help the scientists bring up more effective intervention and treatment strategies. New formulation approaches have been investigated to precisely deliver therapeutics to inflammatory lesions in the pancreas, and some even could directly attenuate the pancreatic damages. In this review, we will briefly introduce the involved pathogenesis and underlying mechanisms of acute pancreatitis, as well as the traditional Chinese medicine and the new drug option. Most of all, we will summarize the drug delivery strategies to reduce inflammation and potentially prevent the further development of pancreatitis, with an emphasis on the bifunctional nanoparticles that act as both drug delivery carriers and therapeutics.
Studies on calf rotavirus in a calf kidney diploid cell line

Besides primary cell cultures, few cell lines support the growth of the bovine rotaviruses with development of cytopathic effect. So a calf kidney diploid cell line was developed which has predominantly fibroblastic morphology. The chromosomal number is 2n = 60 with 29 pairs of autosomes and two "X" chromosomes. The cell line supported good replication of bovine rotaviruses with development of cytopathic effect. In the absence of trypsin the cells did not support the productive replication of bovine rotaviruses present in 12 separate samples of calf diarrhoea, although development of cell-associated viral antigen was recorded up to seventh passage in 3 samples. The cells also supported the replication of bovine rhinotracheitis virus, a bovine enterovirus and poliovirus (type 1 vaccine strain). In each case there was development of cytopathic effect. A plaque assay for bovine rotaviruses was developed which worked well within narrowly defined limits. The reproducibility of the plaque test has made it possible to examine the kinetics of heat and serum inactivation. The virus was neutralised readily by homologous antirotavirus serum than heterologous serum and similar observations were also made between two bovine rotaviruses (the U. K. and the Northern Ireland strains). The two strains are being differentiated on the basis of their plaque morphology and serum neutralisation kinetics by homologous and heterologous serum. The presence of higher salt concentrations in the medium during virus adsorption, was found to shorten the replication cycle of virus. However, when the cells were grown in Earle's rather than Hank's balance salt solution in the base medium, the replicative cycle of virus was shortest (20 hours) with virtual destruction of cell monolayer in 24 hours. The role of calcium in rotavirus replication was emphasized and the isolation of rotaviruses in primary monkey kidney cells with higher concentrations of Ca[2+] in the medium is proposed.
The Impact of Mastitis on the Biochemical Parameters, Oxidative and Nitrosative Stress Markers in Goat’s Milk: A Review

Goat mastitis has become one of the most frequently diagnosed conditions in goat farms, with significant economic impact on the dairy industry. Inflammation of the mammary gland poses serious consequences on milk composition, with changes regarding biochemical parameters and oxidative stress markers. The aim of this paper is to present the most recent knowledge on the main biochemical changes that occur in the mastitic milk, as well as the overall effect of the oxidative and nitrosative stress on milk components, focusing on both enzymatic and nonenzymatic antioxidant markers. Mastitis in goats is responsible for a decrease in milk production, change in protein content with pronounced casein hydrolysis, and reduction in lactose concentration and milk fat. Milk enzymatic activity also undergoes changes, regarding indigenous enzymes and those involved in milk synthesis. Furthermore, during mastitis, both the electrical conductivity and the milk somatic cell count are increased. Intramammary infections are associated with a reduced milk antioxidant capacity and changes in catalase, lactoperoxidase, glutathione peroxidase or superoxide dismutase activity, as well as reduced antioxidant vitamin content. Mastitis is also correlated with an increase in the concentration of nitric oxide, nitrite, nitrate and other oxidation compounds, leading to the occurrence of nitrosative stress.
The dysregulation of monocyte subpopulations in individuals at risk of developing rheumatoid arthritis.

OBJECTIVES Individuals carrying antibodies against citrullinated proteins (ACPA) are at high risk of developing RA. EULAR provided a clinical definition of individuals with arthralgia suspicious for progression to RA (clinically suspect arthralgia, CSA). The alteration of monocyte subpopulations in patients with established RA has been previously described. We analysed peripheral blood monocyte subpopulations in individuals with arthralgia at risk of RA. METHODS We included 70 at-risk individuals, defined as having arthralgia without arthritis and being either ACPA+ or meeting the clinical CSA definition, 23 patients with early RA (ERA) and 19 healthy controls (HCs). Monocytes classified as classical (CD14++CD16-), intermediate (CD14++CD16+/++) and nonclassical (CD14-/+CD16++) were analysed by flow cytometry. RESULTS Of the 70 at-risk individuals, 46 were ACPA+ and 45 met the CSA definition. The at-risk individuals and, especially, ERA patients had a lower percentage of classical monocytes and a higher percentage of nonclassical monocytes than the HCs. ACPA positivity had no effect on the difference in the distribution of the monocyte subsets between at-risk individuals and ERA patients, but a difference was determined in those reaching the ERA phase. However, when compared with HCs, the shift of monocyte subsets was more significant in ACPA+ than in ACPA- individuals with arthralgia. This trend was observed in individuals who did not meet the CSA definition. This finding was, however, determined by a selection bias, as these individuals were solely ACPA+. CONCLUSION The shift from classical to nonclassical monocyte subpopulations was observed already in individuals at risk of developing RA.
Estimation of Sodium and Potassium Intake: Current Limitations and Future Perspectives

Globally, average dietary sodium intake is double the recommended amount, whereas potassium is often consumed in suboptimal amounts. High sodium diets are associated with increased cardiovascular and renal disease risk, while potassium may have protective properties. Consequently, patients at risk of cardiovascular and renal disease are urged to follow these recommendations, but dietary adherence is often low due to high sodium and low potassium content in processed foods. Adequate monitoring of intake is essential to guide dietary advice in clinical practice and can be used to investigate the relationship between intake and health outcomes. Daily sodium and potassium intake is often estimated with 24-h sodium and potassium excretion, but long-term balance studies demonstrate that this method lacks accuracy on an individual level. Dietary assessment tools and spot urine collections also exhibit poor performance when estimating individual sodium and potassium intake. Collection of multiple consecutive 24-h urines increases accuracy, but also patient burden. In this narrative review, we discuss current approaches to estimating dietary sodium and potassium intake. Additionally, we explore alternative methods that may improve test accuracy without increasing burden.
Variability in Morpho-biochemical Traits Associated with Pod Borer (Helicoverpa armigera) Resistance in Pigeonpea Pods

Pigeonpea contributes to food and nutrition security among poor households in urban and rural areas. Globally it is characterized by stagnant and unstable yield due to its susceptibility to various stresses including the pod borer (Helicoverpa armigera) which causes substantial damage to the crop and may result in absolute economic yield loss. The existing cultivated pigeonpea are susceptible to pod borer with only a few genotypes reported to be tolerant based on pod and seed damage. Limited information is available on morphological and biochemical traits associated with pod borer resistance among the existing genotypes. This study was therefore carried out to identify diversified sources of resistance against pod borer damage. The study was set up to assess traits that may contribute to pod borer resistance among 12 selected elite pigeonpea genotypes in three replicates and means were separated based on LSD test using Genstat software. The field study was carried out in Kerio Valley during the long rains of April-September of 2017. The genotypes varied significantly for all the parameters measured at P≤0.05 with a mean of 608.33 g/100 g (crude protein), 175.61 mg/100 g (total phenol), 19.85 mg/100 g (total flavonoid), 0.448 mm (trichome length), 210.6 / 4 mm2(trichome density) and 0.353 mm(depth of locules). Significant negative correlation was also observed between total phenol, total flavonoid, depth of locules, trichome length and trichome density with pod damage. However, a positive correlation was recorded between crude proteins with pod damage. These results reveal that, host plant resistance is an association of several morphological and biochemical traits. Therefore, these genotypes with elevated levels can be selected and utilized in breeding towards improving resistance to pod borer in pigeonpea.
The Application of Yoga Pranayama and Gym Ball Exercise on Labor Pain During Active Phase at Pmb Desa Blahkiuh, Abiansemal, Badung

Pain Labor is a physiological and individual experience. The cause of pain in labor is a combination of ischemia (hypoxia) the muscles of the uterus and stretching that occurs in the lower segment of the uterus (then the cervix). Non-pharmacological efforts that can be done in reducing pain in the mother in the face of labor are through the Application of Yoga Pranayama and Gym Ball Exercise. The purpose of this study was to determine the effect of the application of Yoga Pranayama and Ball Exercise Gym on Labor Pain Active Phase in PMB Blahkiuh Village, Abiansemal, Badung. This research method used the Quasi Experimental Design study with Nonequivalent Control Group Design. The approach used is a prospective approach. Sample consisted 40 respondents. Respondent selected by purposive sampling and divide into control group and intervention group. Respondents in intervention group were given Yoga Pranayama and Gym Ball Exercise, while in the control group respondents were given conventional therapy. The data were analyzed using the Paired T-test to compare the results of the pre-test and post-test, and to compare intervention and control groups use Independent T-Test. Based on statistical tests obtained all p values <0.05 means that there is a significant difference between pain labor in the control and intervention group, so it can be interpreted that pain scale in intervention group is lower than control group. The conclusion of this study was there’s influence on the application of Yoga Pranayama and the Ball Exercise Gym on Labor Pain in the Active Phase in PMB Blahkiuh Village, Abiansemal, Badung.
Comparison of Whole and Gutted Baltic Herring as a Raw Material for Restructured Fish Product Produced by High-Moisture Extrusion Cooking

Interest in using undervalued forage fish for human consumption has recently increased due to its environmental benefits. However, feasible strategies to process the undervalued fish species to food use are limited. Therefore, this study investigated the possibility to utilise whole (ungutted) Baltic herring as a raw material for hybrid plant-fish meat analogues produced by high-moisture extrusion cooking. The sample properties were compared with ungutted Baltic herring. Produced meat analogues showed sufficiently high microbial quality, with spoilage microbes showing growth levels of under 1.4 log CFU/g. Whole fish and gutted fish extrudates showed uniform flavour- and odour-related sensory profiles. Colour values of the whole fish (L* 57.8) extrudates were similar to the values of gutted fish extrudates (L* 62.0). The whole and gutted fish extrudates had tensile strength in a cross-cut direction of 25.5 and 46.3 kPa, respectively. This correlated with the tearing force of the extrudates analysed by a trained sensory panel. Furthermore, a more explicit protein network was microscopically observed in gutted fish than in whole fish extrudates. The present study showed that high-moisture extrusion cooking enables the use of whole small-sized fish for human consumption.
EFFECT OF ROLE CONFLICT AND ROLE OVERLOAD TO BURNOUT AND ITS IMPACT ON CYBERLOAFING (STUDY ON PT PLN (PERSERO) PUSAT MANAJEMEN KONSTRUKSI)

Cyberloafing is one of the employee's counterproductive behavior by using mobile phone and office internet facility for personal use during working hours. Cyberloafing behavior is caused one of them by the psychological condition of employees, namely burnout. The cause of this burnout is due to role conflict and role overload. If the behavior of cyberloafing continues to be done will hamper the productivity of employees and eventually will have a negative impact on the company. The problem in this research is how to decrease cyberloafing by seeing role conflict, role overload, and burnout. The sample of this study amounted to 150 respondents. Sample determination was done by census technique. Respondents in this research are employees of PT PLN (Persero) Pusat Manajemen Konstruksi Semarang. The analysis tool used is Structural Equation Modeling (SEM) in AMOS 22 program. Based on five hypotheses tested, there are four accepted hypotheses. Role conflict has a significant effect on cyberloafing, role overload has no effect on cyberloafing, role conflict and burnout has a significant effect on burnout, and burnout has a significant effect on cyberloafing. In an effort to decrease the level of cyberloafing, good human resource management is required. Purpose of Human resource management is to avoid role conflict, role overload, and burnout affecting cyberloafing.