Miracle-dz/UniFAR-FTUs huggingface dataset:
A Sodium-Translocating Module Linking Succinate Production to Formation of Membrane Potential in Prevotella bryantii

Feeding strategies for ruminants are designed to optimize nutrient efficiency for animals and to prevent energy losses like enhanced methane production. Key to this are the fermentative reactions of the rumen microbiota, dominated by Prevotella spp. ABSTRACT Ruminants such as cattle and sheep depend on the breakdown of carbohydrates from plant-based feedstuff, which is accomplished by the microbial community in the rumen. Roughly 40% of the members of the rumen microbiota belong to the family Prevotellaceae, which ferments sugars to organic acids such as acetate, propionate, and succinate. These substrates are important nutrients for the ruminant. In a metaproteome analysis of the rumen of cattle, proteins that are homologous to the Na+-translocating NADH:quinone oxidoreductase (NQR) and the quinone:fumarate reductase (QFR) were identified in different Prevotella species. Here, we show that fumarate reduction to succinate in anaerobically growing Prevotella bryantii is coupled to chemiosmotic energy conservation by a supercomplex composed of NQR and QFR. This sodium-translocating NADH:fumarate oxidoreductase (SNFR) supercomplex was enriched by blue native PAGE (BN-PAGE) and characterized by in-gel enzyme activity staining and mass spectrometry. High NADH oxidation (850 nmol min−1 mg−1), quinone reduction (490 nmol min−1 mg−1), and fumarate reduction (1,200 nmol min−1 mg−1) activities, together with high expression levels, demonstrate that SNFR represents a charge-separating unit in P. bryantii. Absorption spectroscopy of SNFR exposed to different substrates revealed intramolecular electron transfer from the flavin adenine dinucleotide (FAD) cofactor in NQR to heme b cofactors in QFR. SNFR catalyzed the stoichiometric conversion of NADH and fumarate to NAD+ and succinate. We propose that the regeneration of NAD+ in P. bryantii is intimately linked to the buildup of an electrochemical gradient which powers ATP synthesis by electron transport phosphorylation. IMPORTANCE Feeding strategies for ruminants are designed to optimize nutrient efficiency for animals and to prevent energy losses like enhanced methane production. Key to this are the fermentative reactions of the rumen microbiota, dominated by Prevotella spp. We show that succinate formation by P. bryantii is coupled to NADH oxidation and sodium gradient formation by a newly described supercomplex consisting of Na+-translocating NADH:quinone oxidoreductase (NQR) and fumarate reductase (QFR), representing the sodium-translocating NADH:fumarate oxidoreductase (SNFR) supercomplex. SNFR is the major charge-separating module, generating an electrochemical sodium gradient in P. bryantii. Our findings offer clues to the observation that use of fumarate as feed additive does not significantly increase succinate production, or decrease methanogenesis, by the microbial community in the rumen.
Fertilization methods and substrate particle size differentially affect growth and macronutrient status of Laelia anceps subsp. anceps

The effects of three fertilization methods (FM) using the Peters Professional® 30N-10P-10K water soluble fertilizer were analyzed: fertilization in irrigation water (FIW), foliar fertilization (FF), and their combination (FIW + FF), as well as two substrate particle sizes (SuPS): larger particle size (LPS) and smaller particle size (SPS), on the growth and macronutrient status of Laelia anceps subsp. anceps (Orchidaceae). A 3 × 2 factorial experiment was established. Aerial and root growth, dry weight and concentration of macronutrients N, P, K, Ca, and Mg in leaves, pseudobulbs, and roots were all evaluated. The interaction of the study factors resulted in a greater number of leaves, while by simple effects, greater leaf area, number of pseudobulbs, root volume, and dry matter of leaves and roots were obtained, when FIW and LPS were used. With FF, greater growth in root volume was observed. There were no differences in the concentration of N in the growth organs analyzed, but due to the interaction of factors, a higher concentration of P, K, Mg, and S was observed in roots, mainly with FIW + FF and SPS; although for P, there was a higher concentration in FIW and LPS. In the case of SPS, the concentrations of K and S were higher in leaves, and in pseudobulbs P, K, Ca, Mg, and S were higher. The results showed that there are positive effects on plant growth when FIW and LPS are used, while higher concentrations of nutrients in leaves, pseudobulbs and mainly in roots are observed when using SPS.
Improvement of Salt Stress Tolerance in Strawberry by Ethyl Methane Sulfonate Treatment

In Egypt, strawberry is one of the most important export vegetable crops and in addition,traded locally wide.Strawberry crop is sensitive to salinity of soil and irrigation water, as an increase in salinity over 700ppm has a negative effect on vegetative growth and yield. This study was conducted to obtain genetic variation and assess their tolerance to salt stress by ethyl methane sulfonate (EMS). EMS with three concentrations (0.0, 0.1 and 0.3 %)for (30, 60 and 90 min) applied to “Fortuna cv. ” runner tips. Strawberry seedlings were planted in the open field to assess vegetative growth and yield traits. Strains were evaluated under saline stress conditions (500 and 1000 ppm of Na Cl) after selecting the best.RAPDPCR technique was used to detect the potentiality variability effects of EMS.The results showed that soaking in EMS treatment improved the vegetative growth and yield characteristics under salt stress conditions compared with the control such as plant height,number of leaves, chlorophyll content, number of fruits/plot and weight of fruits/plot. The results showed also high values for fruit quality parameters such as. ,fats (%), proteins (%), ash (%), humidity (%),carbohydrates (%)and fibers (%)with EMS treatment compared to control plants. Using four RAPD primers, a total number of 15polymorphic bands were detected, out of 12 bands were resulted after EMS treatment. Eleven unique bands were observed, which eight from EMS treatment plants and other three bands amang the contral plants.This study revealed that EMS,as a chemical mutagen is considered is promising in chemical enhancing salt tolerance.
Impact of Thickness on the Optical Properties of Selenium Thin Films

Objectives: The primary objective of this investigation is to make a systematic study on the impact of thickness on optical properties, such as energy gap, absorption coefficient, optical density etc., for selenium thin films. Understanding of the band gap energy and its influence on film thickness is of utmost importance in acquiring the intended electrical characterization of semiconducting films. Materials and methods: Ultra-purity selenium (99.99 %) was deposited on glass substrates. During deposition, the glass substrate with its holder were rotated with constant speed to have a smooth coating. Results and discussions: The XRD findings indicate that selenium is amorphous in nature. The optical band gap energy is found to be decreasing form (2.3 to 2eV) with the rise of film thickness in interval (200 to 1000 nm). The band gap energy obeys inverse square law with respect to thickness. Conclusion: We have properly grown thin films of Se below the De Broglie wavelength limit by thermal evaporation in vacuum. The optical density varies directly with film thickness. The absorption coefficients were in the interval (0.5 to 4) × 107m-1. The AFM results confirmed that the Se nano-size increases with the increase in thickness. Both the grain boundaries and sub-grain regions are clearly visible in the SEM micrographs
Microbial respiration in the asteroid diffusive boundary layer influenced sea star wasting disease during the 2013-2014 northeast Pacific Ocean mass mortality event

Sea star wasting disease (SSWD) describes a suite of abnormal signs in affected Asteroidea (Echinodermata). The disease gained prominence in 2013−2014 after it was linked to mass mortality in the northeast Pacific Ocean. Recent work highlighted the key role of heterotrophic microorganisms inhabiting the diffusive boundary layer around sea stars in generating disease signs via oxygen depletion. However, it is unclear whether this phenomenon occurred during the 2013−2014 mass mortality or how surviving populations may have adapted to low oxygen conditions. In this opinion piece, I outline evidence for this phenomenon in both oceanographic conditions experienced by asteroids in 2013−2014 and from population genetic studies of surviving asteroids.
Study of electrocardiographic changes in patients with acute cerebrovascular accidents

Background: Stroke is “a focal (or at times global) neurological impairment of sudden onset, and lasting more than 24 hours (or leading to death), and of presumed vascular origin. A variety of cardiovascular events like cardiac arrest, arrhythmias, and severe hypotension can be seen in stroke victims. The purpose of this study is to investigate the prevalence and characterization of ECG abnormalities in patients of cerebrovascular accidents.Methods: Study was carried out in Dr. PDMMC and tertiary care hospital Amravati, Maharashtra, India. All acute cerebrovascular accidents patients were the study subjects. The diagnosis of ischemic stroke was confirmed by CT scanning. Pre-validated questionnaire was used for data collection. At the end of the study all the ECG were compared to approach towards the result. Study was carried out over a period of three months from October to December 2020.Results: We have recruited 90 stroke patients, most of them were males. Major type was ischemic stroke. In total 62 (68.89%) stroke patients had some form of ECG change. Majority i.e. 35 (38.89%) patients had QTc prolongation followed by 32 (35.56%) patients had T wave changes. QTc prolongation and Atrial fibrillation were significantly more among hemorrhagic stroke patients (p<0.05) and T wave changes and ST changes (elevation or depression) were significantly more among ischemic stroke patients (p<0.05). Conclusions: Prevalence of ECG abnormalities among stroke patients was more i.e. 68.89%. QTc prolongation and T wave changes were the commonest ECG abnormalities.
Social capital and knowledge sharing in the public sector

explicit knowledge sharing; public sector; social capital; tacit knowledge sharing This research proposes and validates a model hypothesizing the relationships between social capital and knowledge sharing in the public sector. We employed a survey design using a questionnaire to collect data utilizing a convenient sampling method. The study gathered 389 questionnaires from civil servants and officials working in departments and agencies in the public sector in Tien Giang province. The research hypotheses were developed and validated using the Structural Model Modeling (SEM) approach. The research results show that all hypothesized relationships were confirmed except the link between structural social capital and tacit knowledge sharing. Our study can be considered a pioneering study to investigate the influence of social capital on both tacit knowledge sharing and explicit knowledge sharing in the public sector. This work also enriches the growing body of knowledge regarding social capital and knowledge sharing in the public sector. Our research also offers suggestions to leaders in the public sector concerning relevant measures and policies that enable employees to share knowledge by promoting social capital.
LIMITATIONS OF THE PANEL REGRESSION MODEL APPLICATION: THE EXAMPLE OF THE WESTERN

Panel regression model may seem like an appealing solution in conditions of limited time series. This is often used as a shortcut to achieve deeper data set by setting several individual cases on the same time dimension, where cross units visually but not really multiply a time frame. Macroeconometrics of the Western Balkan region assumes short time series issue. Additionally, the structural brakes are numerous. Panel regression may seem like a solution, but there are some limitations that should be considered. © 2020 ACE. All rights reserved.
Poly(A) tail length is a major regulator of maternal gene expression during the mammalian oocyte-to-embryo transition

Transcription is silent during the mammalian oocyte-to-embryo transition (OET) until zygotic genome activation (ZGA). Therefore, the OET relies on post-transcriptional regulation of maternal mRNA, among which poly(A) tail lengths have been found to regulate translation for a small number of genes1–3. However, transcriptome-wide poly(A) tail length dynamics and their role in gene expression during the mammalian OET remain unknown. Here, we quantified transcriptome-wide mRNA poly(A) tail length dynamics during the mammalian OET using PAIso-seq1 and PAIso-seq24,5, two methods with different underlying principles that preserve the poly(A) tail information. We revealed that poly(A) tail length was highly dynamic during the mouse OET, and Btg4 is responsible for global maternal mRNA deadenylation. We found that the poly(A) tail length positively associated with translational efficiency transcriptome-wide in mouse oocytes. In addition, genes with different alternative polyadenylation isoforms show longer poly(A) tails for isoforms with distal polyadenylation sites compared to those with proximal polyadenylation sites in mouse, rat, pig and human oocytes after meiotic resumption, which is not seen in cultured cell lines. Surprisingly, mammalian embryos, namely mouse, rat, pig, and human embryos, all experience highly conserved global mRNA re-polyadenylation after fertilization, providing molecular evidence that the early embryo development before ZGA is driven by re-polyadenylated maternal mRNAs rather than newly transcribed mRNAs. Together, our study reveals the conserved mRNA poly(A) tail length landscape. This resource can be used for exploring spatiotemporal post-transcriptional regulation throughout the mammalian OET.
Plastid-associated galactolipid composition in eyespot-containing dinoflagellates: a review

Relative to the large number of photosynthetic dinoflagellate species, only a select few possess proteinaceous, carotenoid-rich eyespots which have been demonstrated in other algae to act in phototactic responses. The proteins comprising the different categories of dinoflagellate eyespots are positioned in or near the peridinin-containing photosynthetic plastid membranes which are composed primarily of two galactolipids, monoand digalactosyldiacylglycerol (MGDG and DGDG). Within eyespot-containing dinoflagellates, this arrangement occurs mostly in those with secondary plastids, although some dinoflagellates with tertiary plastids of diatom origin are known to possess eyespots. We here provide an examination of the MGDG and DGDG composition of eyespot-containing dinoflagellates with secondary, peridinin-containing plastids and tertiary plastids of diatom origin to address the fundamental question of whether eyespots and their component proteins and carotenoids are associated with alterations in galactolipid composition when compared to eyespot-lacking photosynthetic dinoflagellates. This is an important question because the dinoflagellate eyespot-plastid membrane system can be considered a more complicated and evolved state of plastid development. Included in this examination are data on the previously unexamined peridininand type A eyespot-containing dinoflagellate Margalefidinium polykrikoides, and the type D eyespot-containing, aberrant plastid “dinotom” Durinskia baltica. In addition, we have reviewed the galactolipid composition of algae from the Chlorophyceae, Cryptophyceae, and Euglenophyceae as a comparison to determine if algal classes apart from the Dinophyceae contain altered galactolipids in association with eyespots. We conclude that the presence of an eyespot in dinoflagellates and other algae is not associated with noticeable changes in galactolipid composition.
Nutritional Efficiency of Eucalyptus Clones Under Water Stress

Abstract Plant health and nutrition are strongly influenced by the availability of water. The objective of this study was to evaluate the different patterns of initial growth and nutritional efficiency of ten drought-stressed Eucalyptus clones. Water stress conditions were induced by osmotic stress, by gradual addition of polyethylene glycol 6000 over a 20-day period. Drought stress significantly reduced the dry matter of leaves, roots, and the whole plant as well as the nutritional efficiency of most clones. Clone PL040 was [. ]
HOTAIR regulates colorectal cancer stem cell properties and promotes tumorigenicity by sponging miR-211-5p and modulating FLT-1

ABSTRACT We intended to investigate the underlying mechanism of action of long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) in colorectal cancer (CRC) progression, especially in tumor cell stemness. For that purpose, different assays were performed such as real-time PCR and western blotting to determine the expression of target genes. Cell stemness was determined by sphere formation assay, flow cytometry assay, and the analysis of stemness‐related markers. The interplay among target genes was evaluated using bioinformatics analyses, luciferase reporter and biotin-labeled RNA pull down assays. We found that HOTAIR was highly expressed and predicted poor prognosis survival in CRC. Downregulation of HOTAIR repressed tumor malignant behaviors and cancer stemness. Mechanistically, HOTAIR facilitated the expression of the microRNA (miR)-211-5p target gene fms-like tyrosine kinase-1 (FLT-1), thereby modulating cancer stem cell (CSC) properties in CRC. We conclude that HOTAIR/miR-211-5p/FLT-1 axis contributes to CRC cancer stemness.
Course Redesign: Implementing Project-Based Learning to Improve Students’ Self-Efficacy

This research examined the effects of a first-year elective course redesign on promoting students’ self-efficacy. By implementing a project-based approach in the course curriculum, the study investigated if, and to what extent, did such redesign improve student belief in their ability to master course-related outcomes. Two­-tailed independent samples t-test at an alpha level of . 01 were conducted to answer the research question and compare self-efficacy of students who completed the course under the traditional curriculum (n = 821) and students who completed the course under the new curriculum (n = 881). The results revealed statistically significant differences in students’ responses for all learning outcomes before and after the curriculum redesign.
Applying Mobile Technology to Sustain Physical Activity After Completion of Cardiac Rehabilitation: Acceptability Study

Background Many patients do not meet the recommended levels of physical activity after completing a cardiac rehabilitation (CR) program. Wearable activity trackers and mobile phone apps are promising potential self-management tools for maintaining physical activity after CR completion. Objective This study aims to evaluate the acceptability of a wearable device, mobile app, and push messages to facilitate physical activity following CR completion. Methods We used semistructured interviews to assess the acceptability of various mobile technologies after participation in a pilot randomized controlled trial. Intervention patients in the randomized controlled trial wore the Fitbit Charge 2, used the Movn mobile app, and received push messages on cardiovascular disease prevention and physical activity for over 2 months. We asked 26 intervention group participants for feedback about their experience with the technology and conducted semistructured individual interviews with 7 representative participants. We used thematic analysis to create the main themes from individual interviews. Results Our sample included participants with a mean age of 66.7 (SD 8.6) years; 23% (6/26) were female. Overall, there were varying levels of satisfaction with different technology components. There were 7 participants who completed the satisfaction questionnaires and participated in the interviews. The Fitbit and Movn mobile app received high satisfaction scores of 4.86 and 4.5, respectively, whereas push messages had a score of 3.14 out of 5. We identified four main themes through the interviews: technology use increased motivation to be physically active, technology use served as a reminder to be physically active, recommendations for technology to improve user experience, and desire for personal feedback. Conclusions By applying a wearable activity tracker, mobile phone app, and push messages, our study showed strong potential for the adoption of new technologies by older adults to maintain physical activity after CR completion. Future research should include a larger sample over a longer period using a mixed methods approach to assess the efficacy of technology use for promoting long-term physical activity behavior in older adults.
Quality of Life Assessment in Pre-Hospital and Hospital Emergency Healthcare Workers: A Pilot Study

Introduction Quality of life (QoL) is an important index of general and mental health. Several studies have demonstrated healthcare staff can provide higher quality services to patients when they have a better QoL. Working in emergency care services has a heavy workload therefore poor QoL can lead to poor quality of service to patients. We aimed to assess the QoL in pre-hospital and hospital emergency healthcare workers in Isfahan province in Iran via the WHOQOL-BREF (World Health Organization Quality of Life) questionnaire. Methods A total of 891 pre-hospital and hospital emergency personnel were selected via the census method. The WHOQOL-BREF questionnaire was filled out by the participants over a period of 1 year. Results A total of 891 subjects participated. About 33.7% of the participants were pre-hospital emergency staff, 59.1% emergency department nurses, 5.3% emergency department physicians, and 1.6% emergency medicine specialists. Approximately 412 participants (48.8%) were women and 469 (53.2%) were men (mean age 37.72 ± 11.02 years). QoL in pre-hospital and hospital emergency personnel had the highest mean score in the general health domain (61.43 ± 21.38) and the lowest mean score in the environmental health domain (48.54 ± 17.62). Social relationships with a mean score of 53.30 ± 23.56 were not significantly different to the average (p>0.05). Workplace and work experience had a significant effect on all domains of QoL. Conclusion Pre-hospital and hospital emergency personnel in Isfahan province had an optimal QoL, except in environmental health. Therefore, to increase the QoL in this area the periodical evaluation of environmental health is recommended. Appropriate training to create workplace adjustment and work experience can also improve QoL.