Miracle-dz/UniFAR-FTUs huggingface dataset:
Gene Expression Profiling of Head and Neck Tumors Identifies FOXP1 and SOX10 Expression as Useful for Distinguishing Ameloblastoma From Basaloid Salivary Gland Tumors

Supplemental Digital Content is available in the text. Odontogenic tumors show considerable morphologic heterogeneity and at times the diagnosis can be challenging. Ameloblastoma, the most common odontogenic tumor, can have morphologic similarity to some salivary gland tumors and therefore we sought to identify biomarkers that might aid in the diagnosis by performing transcriptome wide gene expression profiling of 80 odontogenic and salivary gland neoplasms. These data identified the FOXP1+/SOX10− expression profile as characteristic of many odontogenic tumors including ameloblastoma but largely absent in salivary gland tumors. We then assessed 173 salivary gland tumors and 108 odontogenic tumors by immunohistochemistry for FOXP1 and SOX10 expression and found that 34/35 (97%) cases of ameloblastomas were diffusely positive for FOXP1 but completely negative for SOX10. None of the basaloid salivary neoplasms (basal cell adenoma, adenoid cystic carcinoma, polymorphous adenocarcinoma, and myoepitheloma) demonstrated FOXP1+/SOX10− expression pattern. Taken together, the results of this study suggest that the FOXP1+/SOX10− immunophenotype is common in odontogenic tumors including ameloblastoma and might be useful distinguishing these from similar appearing basaloid salivary gland tumors.
Relationships Between Muscle Architecture, Deadlift Performance, and Maximal Isometric Force Produced at the Midthigh and Midshin Pull in Resistance-Trained Individuals

Abstract Bartolomei, S, Rovai, C, Lanzoni, IM, and di Michele, R. Relationships between muscle architecture, deadlift performance, and maximal isometric force produced at the midthigh and midshin pull in resistance-trained individuals. J Strength Cond Res 36(2): 299–303, 2022—The aim of this study was to investigate the relationships between muscle architecture, lower-body power, and maximal isometric force produced at midthigh pull (MTP), and at midshin pull (MSP). Twenty experienced resistance-trained men (age = 25.5 ± 3.2 years; body mass = 86.9 ± 12.4 kg; body height = 178.0 ± 5.3 cm) were tested for deadlift 1 repetition maximum (1RM), countermovement jump (CMJ), peak force (PF), and rate of force development (pRFD20) produced at isometric MTP and isometric MSP. Subjects were also assessed for architecture of vastus lateralis (VL). Physiological muscle thickness, pennation angle, and fascicle length (FL) were measured. Pearson's correlation coefficients were calculated to assess the relationships between variables. In addition, differences between MTP and MSP were assessed using paired-sample t-tests. A significant (p < 0.05) difference was detected on the correlation between deadlift 1RM and MSP (r = 0.78; p < 0.001) compared with MTP (r = 0.55; p = 0.012). Moderate correlations were observed between MSP PF and VLFL (r = 0.55; p = 0.011). Midshin pull pRFD20 was the only parameter significantly correlated with CMJ (r = 0.50; p = 0.048). Significantly higher PF and pRFD20 were recorded in MTP compared with MSP (p = 0.007 and p = 0.003, respectively). The present results show that force produced from the floor position may be more important than force produced from a position that mimics the second pull of the clean for deadlift and vertical jump performances. Coaches and scientific investigators should consider using MSP to assess isometric PF using a test correlated with both muscle architecture and dynamic performances.
Non-antimicrobial approaches at drying-off for treating and preventing intramammary infections in dairy cows. Part 1. Meta-analyses of efficacy of using an internal teat sealant without a concomitant antimicrobial treatment

Abstract Use of antimicrobial approaches at drying-off for preventing new intramammary infections (IMI) during the dry period in dairy cows could be replaced by non-antimicrobial approaches. Such approaches would be of interest not only for organic but also for conventional dairy producers. The objective of the current review was to quantify the effect of non-antimicrobial internal teat sealant (ITS)-based approaches at drying-off for treating and preventing IMI, when compared with no treatment or with an antimicrobial-based approach. The protocol for this review was published before initiating the review. A total of 18 trials from 16 articles could be used to investigate the effect of an ITS-based approach. With the available results, we conclude with a high level of confidence that non-antimicrobial ITS-based dry-off approaches are efficient for preventing new IMI during the dry period when compared with no treatment, and would reduce risk of new IMI by 52%. Moreover, we are relatively confident that a bismuth subnitrate-based ITS performed better than an antimicrobial for preventing new IMI during the dry period (a risk reduction of 23%). Similarly, we are relatively confident that an ITS-based approach would only slightly or not at all reduce the prevalence of IMI at calving compared with untreated quarters.
Posterior Reversible Encephalopathy Syndrome and Idiopathic Noncirrhotic Portal Hypertension in a Patient With Systemic Sclerosis.

P osterior reversible encephalopathy syndrome (PRES) is a neurological syndrome characterized by increased white matter vasogenic edema, typically in the bilateral parieto-occipital regions. The symptoms include headache, altered mental status, vision changes, and possible seizure. This condition has been associated with multiple triggers, most commonly abrupt hypertension, renal insufficiency, cytotoxic drugs, preeclampsia/eclampsia, systemic infections, transplantation, and autoimmune disease. Idiopathic noncirrhotic portal hypertension (INCPH) is characterized by intrahepatic portal hypertension in the absence of cirrhosis or other causes of liver disease and splanchnic venous thrombosis. This is a rare disease that can be secondary to systemic sclerosis (SSc). Here, we describe a patient with SSc and INCPH presenting with headache and vomiting, whose neuroimaging results were consistent with PRES. A 51-year-old woman with a history of splenectomy, esophagogastric varices, repeated upper gastrointestinal bleeding, and SSc was admitted to the hospital due to headache accompanied with nausea and vomiting that were present for 3 days. Her blood pressure on admission was 136/90 mmHg. No nervous system abnormalities were observed. The patient exhibited Raynaud phenomenon in both hands and had swollen fingers with sclerotic skin. The laboratory test results showed the following: hemoglobin level, 90 g/L; platelet count, 395,000/μL; serum iron level, 4.2 μmol/L; and a positive fecal occult blood test. Infection screening, thyroid function, liver function, renal function, and electrolyte panel test results all showed normal values. Immuno-screening showed the patient to be positive for anti–nuclear antibodies (centromere and cytoplasmic patterns) and anti–centromere antibodies. The lumbar puncture results showed normal pressurewith no abnormalities in cerebrospinal fluid, as indicated by biochemical and bacteriological tests. Abdominal color Doppler ultrasound showed cavernous degeneration of the portal vein and gastric varices, whereas an abdominal computed tomographic scan showed cavernous degeneration of the portal vein, thickening of mesenteric vessels, and effusion of the peritoneal cavity. Cranial magnetic resonance imaging scans revealed multiple patchy areas in T2weighted and FLAIR images (Fig. 1). The patient was treated with a low dose of oral prednisone and nimodipine to relieve vasospasm and benazepril to improve her blood pressure. The results of a magnetic resonance imaging scan performed 2 weeks after discharge revealed that the original abnormalities had completely resolved (Fig. 2).
Increased expression of Psoriasin is correlated with poor prognosis of bladder transitional cell carcinoma by promoting invasion and proliferation

Psoriasin, otherwise known as S100A7, is a member of the S100 protein family. With the key function of binding calcium, it is able to regulate a range of cellular functions. Altered Psoriasin expression is associated with poor clinical outcomes in several solid cancers. The present study aimed to examine the implication of Psoriasin in bladder cancer (BC). Expression of Psoriasin was examined in BC cell lines using PCR. Immunohistochemical (IHC) staining of Psoriasin was performed on a bladder disease spectrum tissue array. Plasmids were constructed to effectively knockdown and overexpress Psoriasin in BC cells and further utilized for in vitro BC cellular function assays. Association between Psoriasin expression and survival of patients with BC was evaluated using Kaplan-Meier survival analysis. Psoriasin was revealed to be expressed by both bladder epithelia and cancer cells as determined by IHC. Increased expression of Psoriasin was significantly correlated with a poor overall BC patient survival. Overexpression of Psoriasin in the EJ138 cell line increased cellular proliferation, adhesion and invasion, whereas knockdown exhibited the opposite effect on cellular functions in RT112 cells. Matrix metalloprotease (MMP)9 appeared to be the most affected of the three MMPs examined in these two BC cell lines. The analysis revealed a positive correlation in BC tumours between Psoriasin and MMP9. Overall, high Psoriasin expression was correlated with poor overall survival in BC patients and promoted invasiveness of BC cells via upregulation of MMPs. Psoriasin possesses certain prognostic and therapeutic potential in BC which requires further exploration.
Trial marketing in the Pan-Canadian Early Detection of Lung Cancer Study

Background: Recruitment to clinical trials is suboptimal, increasing costs, and delaying the potential implementation of clinical advances. Among other barriers, the lack of marketing experience among trialists may limit recruitment. In this observational study, in the context of the Pan-Canadian Early Detection of Lung Cancer Trial, we assessed the value of a motivational survey of study participants in planning a tailored advertising campaign and analysed the value of individual components of advertising in generating telephone calls to the study and recruited subjects. Methods: The Pan-Canadian Early Detection of Lung Cancer Trial was a single arm study assessing risk modelling for lung cancer screening by low-dose computed tomography scan and autofluorescence bronchoscopy. Individuals were recruited to eight sites across Canada without a central marketing plan. On contact with the study, individuals reported how they heard about the study according to a predefined list. One site, the Juravinski Cancer Centre, worked with a marketing expert to develop a survey to assess participant motivations, source of study awareness, and personal habits. The survey was used to develop a media campaign for recruitment. Media events were collected from all sites. The primary analysis assessed the number of telephone contacts and recruited subjects associated with various media factors. Individual print media characteristics were assessed for their effect on recruitment. Results: At all sites, 7059 individuals contacted the study, and 2537 were eligible and recruited. Among 52 individuals completing the Juravinski Cancer Centre survey, motivation included concern for personal risk of lung cancer (71%), followed by desire to contribute to a cure (67%), followed by personal knowledge of a person with lung cancer (50%). Most reported hearing of the study from the newspaper (58%) despite no print ad yet being distributed. With survey input, a newsprint campaign was executed. The number of media events varied by site (median: 13, range: 3–28). Among all recruits, 56.4% reported referral by newspaper followed by family/friend (14%). Telephone contacts and recruited subjects per event varied significantly by site, while unpaid media events appeared superior to paid events. Print media characteristics associated with increased telephone contacts and recruitment included use of a rational appeal (vs a mixed rational–emotional), less use of white space, and larger headline font. Conclusion: A survey of trial candidates provides useful information regarding personal motivation, media use, and lifestyle. Unpaid media events appear superior in generating recruitment, while print media may be superior to radio and television in selecting eligible recruits. The utility of individual print media characteristics appears to differ from the commercial advertising literature. Further research on marketing in clinical trials is encouraged to improve recruitment (ClinicalTrials.gov registration: NCT00751660, https://clinicaltrials.gov/ct2/show/NCT00751660).
Groin Injuries in Soccer: Investigating the Effect of Age on Adductor Muscle Forces.

PURPOSE The sudden rise in the injury incidence during adolescence, is also evident in soccer related injuries to the groin. Submaximal passing applies high stress on the adductor muscles and pubic symphysis and is therefore likely to be connected to the occurrence of groin injuries. Therefore, the purpose of the study was to compare hip joint kinematics and adductor muscle forces of different adolescent age groups during submaximal soccer passing. METHODS Sixty participants, in four groups, below 12, 15, 16 and 23 years (U12, U15, U16, U23), were analyzed. A Footbonaut, equipped with a 3D motion capture system consisting of 16 cameras, was used to capture kinematic data of short passes. Inverse dynamic analysis was performed to calculate muscle forces of ten passes of each subject. RESULTS The U15 group showed reduced angular velocities. A rise in hip adductor muscle forces was evident from the youngest group up to the oldest groups. The largest increase (49%) was found between U12 and U15. Lower limb mass was identified as the best predictor for the increasing adductor force. CONCLUSION The reduced angular velocities of the U15 and the increase in muscle forces between all age groups was attributed to the increasing segment masses and length. This increases the moments of inertia of the leg segments thereby demanding higher forces to accelerate the segments. Most likely, the stress put upon the adductors apophyses increases during adolescence, as tendons are known to adapt slower than muscles, increasing the risk for overuse injuries. Coaches could use lower limb mass as an indicator for fast increases in the force demand to identify players who would benefit from a reduced training volume.
Acetylation and insulin resistance: a focus on metabolic and mitogenic cascades of insulin signaling

Abstract Insulin resistance is associated with an increased risk of several metabolic disorders including type 2 diabetes, hypertension and cardiovascular diseases. Advances over the last decade have expanded our understanding of the molecular mechanisms underlying insulin resistance; however, many details of the mechanisms causing insulin resistance remain unknown. Recently, attention has shifted toward the role of epigenetics in insulin resistance. In this regard, acetylation of the histone tails has been widely investigated for its role in influencing both metabolic and mitogenic cascades of insulin signaling. More specifically, histone acetyltransferases and histone deacetylases, as major modulators of chromatin accessibility and gene expression, have been studied to determine a possible interconnectivity between the special effects of lysine acetylation status and tyrosine phosphorylation networks on the target proteins of downstream pathways involved in both metabolic and mitogenic cascades of insulin signaling. There is accumulating evidence for the post-translational modification effects of IGFR, InsR, IRS1/2, PI3K, Akt, GLUT4, FoxO, PGC-1α, PPAR, AMPK and MAPKs on insulin resistance and glucose homeostasis. In this paper, we review the importance of acetylation of these factors in the regulation of insulin signaling and glucose metabolism, with a primary focus on the target proteins of downstream signaling of insulin. We also provide an update on the interplay between epigenetic modification and the cellular genome in the context of insulin signaling and describe the possible effect of the environment on this epigenetic regulation.
Tibial Inlay Posterior Cruciate Ligament Reconstruction

Reconstruction of the posterior cruciate ligament is typically made through either tibial inlay or transtibial methods. Double-bundle reconstruction can be combined with either technique and has clear biomechanical advantages. On the basis of prior evidence and the author’s own surgical experiences, this technique paper provides the reasoning for using these methods. Further evidence and reasoning is given for when to use the inlay technique rather than the transtibial technique for posterior cruciate ligament reconstruction.
A multicenter, prospective, randomized trial of pain and discomfort during maxillary expansion: Leaf expander versus Hyrax expander.

BACKGROUND Pain suffered by the young patient is the most frequent symptom during orthodontic treatment and is the one that most frightens children and causes worry in their families. AIM to investigate pain perception and function impairment during the first week of activation of two palatal expansion screws. DESIGN 101 subjects were randomly divided into two groups: RME group included patients treated with the standard hyrax expansion screw and LEAF group included patients treated with Leaf Expander appliance. Pain intensity was assessed via the Wong-Baker scale. A questionnaire on oral function impairments was also compiled by the patients. RESULTS The pain Scale analysis showed that patients in the RME group suffered from a significantly higher level of pain than those in the LEAF group (88.6% vs 25%, p<0.01). RME group showed highest pain indexes from day 1 to day 4 (51,4% RME vs 9,7% LEAF suffered at least once from strong pain in the first four days, p<0.01). Furthermore, oral functions were similarly affected in both groups. CONCLUSIONS Pain reported during maxillary arch expansion is influenced by clinical activation protocol and by the screw type. Patients treated with Leaf Expander reported significantly lower pain level in the first 7 days of treatment.
PCSK9 inhibitors for treating hypercholesterolemia

ABSTRACT Introduction: Scientific evidence on subjects treated with statin or other lipid-lowering treatments has established that treatments aiming to lower low-density lipoprotein cholesterol (LDL-C) can reduce atherosclerosis. PCSK9 inhibitors (PCSK9-i), thanks to their efficacy in reducing LDL-C constitute a further step in the treatment of dyslipidemia and cardiovascular (CV) diseases. Areas covered: The purpose of this narrative review is to summarize the current knowledge of PCSK9-i, with particular regard to pharmacodynamic, pharmacokinetic, and clinical data on evolocumab and alirocumab. Expert opinion: PCSK9-I are effective in reducing atherosclerotic events through their significant LDL-C-lowering action similarly to statins. Furthermore, these drugs can be considered safe and well-tolerated. However, some controversies remain with regard to their efficacy in reducing mortality and the paucity of data on both pleiotropic effects and long-term safety of these drugs. However, future studies will focus on understanding the effects of very low cholesterol levels on health. At present, we know that the genetic model of PCSK9 deficiency is characterized by very low LDL-C levels without particular health problems. Yet, we do not know the effect of prolonged PCSK9 inhibition induced by antibody action during the lifetime of normal subjects.
Examination of Sleep and Injury Among College Football Athletes.

Burke, TM, Lisman, PJ, Maguire, K, Skeiky, L, Choynowski, JJ, CapaldiII, VF, Wilder, JN, Brager, AJ, and Dobrosielski, DA. Examination of sleep and injury among college football athletes. J Strength Cond Res XX(X): 000-000, 2019-The purpose of this study was to characterize subjective sleep metrics in collegiate football players at the start of the season, determine the relationship between preseason subjective sleep measures and in-season objective sleep characteristics, and examine the association between subjective and objective sleep metrics and incidence of time-loss injury during the competitive season. Ninety-four Division I football players completed 5 validated sleep-related questionnaires to assess sleep quality, insomnia severity, daytime sleepiness, sleep apnea risk, and circadian preference before the start of the season. Clinical thresholds for sleep questionnaires were used to determine risk of sleep disorders. Continuous wrist actigraphy was collected throughout the season to generalize sleep behaviors. Time-loss injury incidence data were recorded and used for analysis. Results indicated that 67.4% (60 of 89) of athletes scored above clinical threshold in at least 1 questionnaire to indicate sleep disorder risk. At the start of the season, players subjectively reported an average sleep duration of 7:16 ± 1:18 hours:minutes, which was in contrast to the 6:04 ± 0:41 hours:minutes measured through actigraphy during the season. Logistic regression models adjusted for age and body mass index revealed no significant associations between injury and subjective (odds ratio [OR] = 1.00; 95% confidence interval [CI] = 0.99-1.01) and objective (OR = 1.01; 95% CI = 0.99-1.02) sleep duration or measures attained from sleep questionnaires (ORs ranged from 1.01 to 2.87). Sleep metrics (quantity and quality) were not associated with increased risk of injury in this cohort of collegiate football players.
Fetal monocytes possess increased metabolic capacity and replace primitive macrophages in tissue macrophage development

Tissue‐resident macrophages (MΦTR) originate from at least two distinct waves of erythro‐myeloid progenitors (EMP) arising in the yolk sac (YS) at E7.5 and E8.5 with the latter going through a liver monocyte intermediate. The relative potential of these precursors in determining development and functional capacity of MΦTR remains unclear. Here, we studied development of alveolar macrophages (AM) after single and competitive transplantation of different precursors from YS, fetal liver, and fetal lung into neonatal Csf2ra−/− mice, which lack endogenous AM. Fetal monocytes, promoted by Myb, outcompeted primitive MΦ (pMΦ) in empty AM niches and preferentially developed to mature AM, which is associated with enhanced mitochondrial respiratory and glycolytic capacity and repression of the transcription factors c‐Maf and MafB. Interestingly, AM derived from pMΦ failed to efficiently clear alveolar proteinosis and protect from fatal lung failure following influenza virus infection. Thus, our data demonstrate superior developmental and functional capacity of fetal monocytes over pMΦ in AM development and underlying mechanisms explaining replacement of pMΦ in fetal tissues.