Miracle-dz/UniFAR-FTUs huggingface dataset:
Wheel and Deal in the Mitochondrial Inner Membranes: The Tale of Cytochrome c and Cardiolipin

Cardiolipin oxidation and degradation by different factors under severe cell stress serve as a trigger for genetically encoded cell death programs. In this context, the interplay between cardiolipin and another mitochondrial factor—cytochrome c—is a key process in the early stages of apoptosis, and it is a matter of intense research. Cytochrome c interacts with lipid membranes by electrostatic interactions, hydrogen bonds, and hydrophobic effects. Experimental conditions (including pH, lipid composition, and post-translational modifications) determine which specific amino acid residues are involved in the interaction and influence the heme iron coordination state. In fact, up to four binding sites (A, C, N, and L), driven by different interactions, have been reported. Nevertheless, key aspects of the mechanism for cardiolipin oxidation by the hemeprotein are well established. First, cytochrome c acts as a pseudoperoxidase, a process orchestrated by tyrosine residues which are crucial for peroxygenase activity and sensitivity towards oxidation caused by protein self-degradation. Second, flexibility of two weakest folding units of the hemeprotein correlates with its peroxidase activity and the stability of the iron coordination sphere. Third, the diversity of the mode of interaction parallels a broad diversity in the specific reaction pathway. Thus, current knowledge has already enabled the design of novel drugs designed to successfully inhibit cardiolipin oxidation.
Predicting knottiness of Pinus sylvestris for use in tree bucking procedures

Abstract The quality and accompanying value of Scots pine (Pinus sylvestris L.) lumber varies markedly in the Nordic countries. Consequently, tree bucking has a great impact on the economic result. The objectives of this study were (1) to analyse which knot characteristics usually reduce pine lumber sawn from the butt-logs to the next quality grade, (2) to determine the most appropriate characteristics that could be used in predicting lumber quality, and (3) to develop models that could be used in practice for predicting the probability of certain quality grades. The study was based on field experiments and test sawing data on 100 Scots pine stems from south-western Finland. Since the results showed that the maximum dead knot is the most crucial knot characteristic when the first cross-cutting point is determined, models were created that predict the height of the first dead knot that lowers grade A to B (B-grade dead knot). Both early growth rate and dead branch height should be measured to predict pine butt-log quality. Early growth rate seems to be appropriate in predicting between-stand variation, while dead branch height is appropriate for predicting within-stand variation.
Application of edible coatings on meats, poultry and seafoods: a review

Edible coatings can improve the quality of fresh, frozen, and processed meat, poultry, and seafood products by retarding moisture loss, reducing lipid oxidation and discoloration, enhancing product appearance in retail packages by eliminating dripping, sealing in volatile flavors, functioning as carriers of food additives such as antimicrobial and antioxidant agents, and reducing oil uptake by battered and breaded products during frying. This paper reviews the application of various types of lipid-, polysaccharide-, and protein-based edible coatings, as well as multicomponent edible coating systems, on meats, poultry, and seafoods.
Use of genetic variation to separate the effects of early and later life adiposity on disease risk: mendelian randomisation study

Abstract Objective To evaluate whether body size in early life has an independent effect on risk of disease in later life or whether its influence is mediated by body size in adulthood. Design Two sample univariable and multivariable mendelian randomisation. Setting The UK Biobank prospective cohort study and four large scale genome-wide association studies (GWAS) consortiums. Participants 453 169 participants enrolled in UK Biobank and a combined total of more than 700 000 people from different GWAS consortiums. Exposures Measured body mass index during adulthood (mean age 56.5) and self-reported perceived body size at age 10. Main outcome measures Coronary artery disease, type 2 diabetes, breast cancer, and prostate cancer. Results Having a larger genetically predicted body size in early life was associated with an increased odds of coronary artery disease (odds ratio 1.49 for each change in body size category unless stated otherwise, 95% confidence interval 1.33 to 1.68) and type 2 diabetes (2.32, 1.76 to 3.05) based on univariable mendelian randomisation analyses. However, little evidence was found of a direct effect (ie, not through adult body size) based on multivariable mendelian randomisation estimates (coronary artery disease: 1.02, 0.86 to 1.22; type 2 diabetes:1.16, 0.74 to 1.82). In the multivariable mendelian randomisation analysis of breast cancer risk, strong evidence was found of a protective direct effect for larger body size in early life (0.59, 0.50 to 0.71), with less evidence of a direct effect of adult body size on this outcome (1.08, 0.93 to 1.27). Including age at menarche as an additional exposure provided weak evidence of a total causal effect (univariable mendelian randomisation odds ratio 0.98, 95% confidence interval 0.91 to 1.06) but strong evidence of a direct causal effect, independent of early life and adult body size (multivariable mendelian randomisation odds ratio 0.90, 0.85 to 0.95). No strong evidence was found of a causal effect of either early or later life measures on prostate cancer (early life body size odds ratio 1.06, 95% confidence interval 0.81 to 1.40; adult body size 0.87, 0.70 to 1.08). Conclusions The findings suggest that the positive association between body size in childhood and risk of coronary artery disease and type 2 diabetes in adulthood can be attributed to individuals remaining large into later life. However, having a smaller body size during childhood might increase the risk of breast cancer regardless of body size in adulthood, with timing of puberty also putatively playing a role.
Elevated plasma levels of exosomal BACE1-AS combined with the volume and thickness of the right entorhinal cortex may serve as a biomarker for the detection of Alzheimer's disease

Long non-coding RNA (lncRNA) and exosomes are involved in the pathological process of Alzheimer's disease (AD), the pathological changes of which are usually first observed in the entorhinal cortex and hippocampus. The aim of the present study was to determine whether the measurement of plasma exosomal lncRNA combined with image data of the entorhinal cortex and hippocampus could be used as a biomarker of AD. A total of 72 patients with AD and 62 controls were recruited, and the expression levels of several lncRNAs were assessed. Of the recruited participants, 22 patients and 26 controls received brain 3D-BRAVO sequence magnetic resonance imaging (MRI) scans, which were analyzed using an automated analysis tool. The plasma exosomal β-site amyloid precursor protein cleaving enzyme-1-antisense transcript (BACE1-AS) levels in patients with AD were significantly higher compared with the controls (P<0.005). Receiver operating characteristic curve analysis revealed that the area under the curve (AUC) was 0.761 for BACE1-AS, the sensitivity was 87.5%, and the specificity was 61.3%. Analysis of MRI images indicated that the right entorhinal cortex volume (P=0.015) and thickness (P=0.022) in patients with AD were significantly smaller. The AUC was 0.688 for the right entorhinal cortex volume, with a sensitivity of 59.1%, and the specificity was 84.6%. The AUC was 0.689 for right entorhinal cortex thickness, with a sensitivity of 80.8%, and the specificity was 59.1%. A series-parallel test which integrated the BACE1-AS with the right entorhinal cortex volume and thickness, raised the specificity and sensitivity to 96.15 and 90.91%, respectively. A logistic regression model demonstrated that combination of the 3 indices provided improved sensitivity and specificity simultaneously, particularly when adjusting for age and sex (AUC, 0.819; sensitivity, 81%; specificity, 73.1%). The results of the present study demonstrated that detection of plasma exosomal BACE1-AS levels combined with the volume and thickness of the right entorhinal cortex may be used as a novel biomarker of AD.
Association between tax on sugar sweetened beverages and soft drink consumption in adults in Mexico: open cohort longitudinal analysis of Health Workers Cohort Study

Abstract Objective To examine changes in categories of soft drink consumption in a cohort of Mexican adults, three years after the implementation of the sugar sweetened beverage tax. Design Open cohort longitudinal analysis. Setting Three waves of the Health Workers Cohort Study, Mexico, spanning 2004 to 2018. Participants 1770 people aged 19 years or older with information on drinks consumption available in at least one of the three cohort waves. Main outcome measure Change in probability of belonging to one of four categories of soft drinks consumption (non, low, medium, high) after the tax was implemented. Heterogeneity of associations by income and education was also assessed. Results Before the implementation of the tax, more than 50% of the participants were medium and high consumers of soft drinks and less than 10% were in the non-consumer category. After the tax was implemented, 43% of the population was categorised as medium or high consumers and the prevalence of non-consumers increased to 14%. Three years after implementation of the tax on 1 January 2014, the probability of being a non-consumer of soft drinks increased by 4.7 (95% confidence interval 0.3 to 9.1) percentage points and that of being a low consumer increased by 8.3 (0.6 to 16.0) percentage points compared with the pre-tax period. Conversely, the probability of being in the medium and high levels of soft drinks consumption decreased by 6.8 (0.5 to 13.2) percentage points and 6.1 (0.4 to 11.9) percentage points, respectively. No significant heterogeneity of the tax across income levels was observed, but stronger effects of the tax were seen in participants with secondary school education or higher, compared with those with elementary school or less. Conclusions The Mexican sugar sweetened beverage tax was associated with a reduction in the probability of consuming soft drinks in this cohort of employees from a healthcare provider. The results cannot be extrapolated to the Mexican population, but they suggest that three years after implementation, the tax had helped to increase the proportion of people who do not consume soft drinks while decreasing the proportion of high and medium consumers.
Chromosome banding and molecular cytogenetics of the Barbary macaque, Macaca sylvanus

Abstract Macaca sylvanus has a diploid chromosome number of 2n = 42. With the exception of chromosome 2 all chromosomes appear to have an identical banding pattern to most other macaque species. This chromosome has a clear band, localized immediately below the centromere; therefore, the karyotype of M. sylvanus more closely resembles that of the African Papionini, which also present a large clearly staining band on chromosome 2. C‐band‐ing intensely stained this band. Fluorescent in situ hybridization (FISH) with human chromosome specific DNA probes to macaque chromosome 2 showed that human probe 7 was located on the whole q arm and on a small part of the p arm, immediately adjacent to the centromere; the remaining part of the p arm was hybridized by the DNA probe for human chromosome 21. It appears clear, from the hybridization pattern in the other primates and in the outgroups examined, that chromosome 7 was probably a syntenic region for many ancestral mammalian karyotypes and a single syntenic region .
Realizing Efficient Security and Privacy in IoT Networks

In recent times, security and privacy at the physical (PHY) layer has been a major issue of several communication technologies which comprise the internet of things (IoT) and mostly, the emerging fifth-generation (5G) cellular network. The most real-world PHY security challenge stems from the fact that the passive eavesdropper’s information is unavailable to the genuine source and destination (transmitter/receiver) nodes in the network. Without this information, it is difficult to optimize the broadcasting parameters. Therefore, in this research, we propose an efficient sequential convex estimation optimization (SCEO) algorithm to mitigate this challenge and improve the security of physical layer (PHY) in a three-node wireless communication network. The results of our experiments indicate that by using the SCEO algorithm, an optimal performance and enhanced convergence is achieved in the transmission. However, considering possible security challenges envisaged when a multiple eavesdropper is active in a network, we expanded our research to develop a swift privacy rate optimization algorithm for a multiple-input, multiple-output, multiple-eavesdropper (MIMOME) scenario as it is applicable to security in IoT and 5G technologies. The result of the investigation show that the algorithm executes significantly with minimal complexity when compared with nonoptimal parameters. We further employed the use of rate constraint together with self-interference of the full-duplex transmission at the receiving node, which makes the performance of our technique outstanding when compared with previous studies.
Altered Expression of Three EGFR Posttranslational Regulators MDGI, MIG6, and EIG121 in Invasive Breast Carcinomas

Epidermal growth factor receptor (EGFR) signalling is a highly regulated process with a tight balance between receptor activation and inactivation in invasive breast carcinomas (IBCs) particularly in triple-negative carcinomas (TNC). Clinical trials using anti-EGFR therapies are actually performed although no activating alterations (mutations, amplifications, or rearrangements) of EGFR have been clearly recognized in order to identify new targeted modalities for IBCs. We explored mammary-derived growth inhibitor (MDGI), estrogen-induced gene-121 (EIG121), and mitogen-induced gene-6 (MIG6), three posttranslational EGFR trafficking molecules implicated in EGFR spatiotemporal regulatory pathway. We quantified MDGI, EIG121, and MIG6 at mRNA levels by using real-time quantitative RT-PCR in a series of 440 IBCs and at protein levels by using immunohistochemistry in a series of 88 IBCs. Results obtained by RT-PCR showed that in IBCs, MDGI, MIG6, and EIG121 mRNA were mainly underexpressed (25.7%, 45.0%, and 16.1%, respectively) particularly in the TNC subtype for EIG121 (60.3%). We also observed mRNA overexpression of MDGI and EIG121, respectively, in 12.7% and 22.3% of IBCs. These altered mRNA expressions were confirmed at the protein level. Some links were found between expression patterns of these three genes and several classical pathological and clinical parameters. Only EIG121 was found to have a prognostic significance (p = 0.0038). Altered expression of these three major EGFR posttranslational negative regulators could create an aberrant EGFR-mediated oncogenic signalling pathway in IBCs. MDGI, MIG6, and EIG121 expression status also may be potential useful biomarkers (sensitivity or resistance) in targeted EGFR therapy.
Second‐line treatment of hepatocellular carcinoma after sorafenib: Characterizing treatments used over the past 10 years and real‐world eligibility for cabozantinib, regorafenib, and ramucirumab

The CELESTIAL, RESORCE, and REACH‐2 trials showed survival benefit of cabozantinib, regorafenib, and ramucirumab, respectively, in hepatocellular carcinoma (HCC) patients treated with sorafenib who had good performance status (ECOG 0‐1) and liver function (Child‐Pugh‐A). This study characterizes subsequent treatments received by HCC patients after sorafenib, and determines the proportion of patients eligible for novel therapies if strict eligibility criteria (SEC) were utilized compared to more liberal modified eligibility criteria (MEC, including ECOG 2, Child‐Pugh‐B7).
Lignans from Euphorbia hirta L.

Abstract Five new lignans, euphorhirtins A–D (1–4), 5-methoxyvirgatusin (5), three artefacts, 7S-ethoxyisolintetralin (6), 7R-ethoxyisolintetralin (7), and 7R-ethoxy-3-methoxyisolintetralin (8), together with 13 known ones (9–21) were isolated from the medicinal plant Euphorbia hirta L. The structures of the compounds were elucidated by means of extensive spectroscopic analysis, including 1D and 2D NMR and HR-ESI-MS experiments. The absolute configurations of compound 1 was determined by ECD calculation. The isolates were evaluated for their inhibitory effects against the proliferation of the cancer cell lines (Hep G2, A549, and DU145) and compounds 14 and 18 showed inhibitory activity against the Hep G2 cells with IC50 values 7.2 ± 0.17 and 8.5 ± 0.36 μM. Graphical Abstract
Tooth development in East African Paranthropus

Abstract A rapid differentiation rate in enamel with a "hyper-thick" enamel was suggested to represent a synapomorphic condition in Paranthropus species. To check this hypothesis we have analysed the enamel microstructure of molar teeth from Shungura Formation (Omo, Ethiopia) attributed, by teeth macrostructure, to P. aethiopicus and P. boisei. The number, course and angles of the enamel-dentine junction of striae of Retzius and the relative importance of cuspal and cervical stages were analysed with a stereomicroscope. We assumed the striae of Retzius are growth markers with a 7-day periodicity. Thus, the features analysed indicate the time of crown formation, the number of ameloblasts active at any one time, the differentiation rate and the relative importance of the number of ameloblasts active at any one time in relation to the overall number of ameloblasts implicated in the total crown formation respectively. Enamel thickness was also measured. Although overlap was found between some characteristics, it was possible to define dissimilar pathways in tooth development. Paranthropus aethiopicus, with a faster differentiation rate and a greater number of ameloblasts active at any given time, have a shorter time of crown formation than P. boisei. At first it seems that hyper-thick enamel is not present and that ameloblasts operate as a whole. In P. boisei the ameloblasts work as successive groups. Thus, if the selection to form teeth quickly and the synapomorphic features in Paranthropus' species, as suggested, are retained, P. aethiopicus would be more specialized than P. boisei.
Workload-indexed blood pressure response to a maximum exercise test among professional indoor athletes.

BACKGROUND Exercise testing is performed regularly in professional athletes. However, the blood pressure response (BPR) to exercise is rarely investigated in this cohort, and normative upper thresholds are lacking. Recently, a workload-indexed BPR (increase in systolic blood pressure per increase in metabolic equivalent of task (SBP/MET slope)) was evaluated in a general population and was compared with mortality. We sought to evaluate the SBP/MET slope in professional athletes and compare it with performance. DESIGN This was a cross-sectional study. METHODS A total of 142 male professional indoor athletes (age 26 ± 5 years) were examined. Blood pressure was measured at rest and during a standardized, graded cycle ergometer test. We assessed the BPR during exercise, the workload, and the metabolic equivalent of task (MET). Athletes were divided into groups according to their SBP/MET slope quartiles (I <4.3; II 4.3-6.2; III >6.2-9; IV >9 mmHg/MET) and compared regarding systolic BP (sBP) and workload achieved. RESULTS Athletes in group I (n = 42) had the lowest maximum sBP (180 ± 13 mmHg) but achieved the highest relative workload (4.2 ± 1 W/kg). With increasing SBP/MET slope, the maximum sBP increased (II (n = 56): 195 ± 15 mmHg; III (n = 44): 216 ± 16 mmHg) and the workload achieved decreased (II: 3.9 ± 0.7 W/kg; III: 3.3 ± 0.5 W/kg). The differences in sBP between these groups were significant (p < 0.001). None of the athletes were assigned to group IV (>9 mmHg/MET). CONCLUSION Athletes in the lowest SBP/MET slope quartile displayed the lowest maximum sBP but achieved a higher workload than athletes classified into the other SBP/MET slope groups. This simple, novel metric might help to distinguish a normal from an exaggerated BPR to exercise, to identify athletes at risk of developing hypertension.
Disruption of red turpentine beetle attraction to baited traps by the addition of California fivespined ips pheromone components

Abstract The red turpentine beetle, Dendroctonus valens LeConte (Coleoptera: Scolytidae), is a common bark beetle species found throughout much of North America. In California, D. valens and the California fivespined ips, Ips paraconfusus Lanier (Coleoptera: Scolytidae), are sympatric and often colonize the same tree. In an unrelated study, we observed that I. paraconfusus attack densities in logging debris were inversely related to D. valens attacks on freshly cut stumps. In this study, we test the hypothesis that allomonal inhibition occurs between these two species. Components of the aggregation pheromone of I. paraconfusus (racemic ipsenol, (+)-ipsdienol, and (–)-cis-verbenol) inhibited the response of D. valens to attractant-baited traps. Substitution of racemic ipsdienol for (+)-ipsdienol did not alter this effect. Doubling the release rate did not enhance inhibition. Racemic ipsdienol was not attractive to I. paraconfusus. Temnochila chlorodia (Mannerheim, 1843) (Coleoptera: Trogositidae), a common bark beetle predator, was attracted to the I. paraconfusus aggregation pheromone. These results could have important implications for the development of an effective semiochemical-based management tool for D. valens. Résumé Le dendroctone rouge de l'épinette, Dendroctonus valens LeConte (Coleoptera : Scolytidae), est une espèce commune de scolyte répandue dans presque toute l'Amérique du Nord. En Californie, D. valens et le scolyte à cinq épines de Californie, Ips paraconfusus Lanier (Coleoptera : Scolytidae), sont sympatriques et colonisent souvent le même arbre. Dans une étude différente, nous avons observé que les densités d'attaque d'I. paraconfusus dans les débris de coupe sont inversement reliées aux attaques de D. valens sur les souches récemment coupées. Dans la présente étude, nous éprouvons l'hypothèse selon laquelle il existe une inhibition de répulsion entre ces deux espèces. Les composantes de la phéromone de rassemblement d'I. paraconfusus (l'ipsénol racémique, l'(+)-ipsdiénol, le (–)-cis-verbénol) inhibent la réaction de D. valens à des pièges munis de substances attractives. La substitution de l'ipsdiénol racémique à l'(+)-ipsdiénol ne modifie pas cet effet. Un taux de libération deux fois plus élevé n'augmente pas l'inhibition. L'ipsdiénol racémique n'attire pas I. paraconfusus. Temnochila chlorodia (Mannerheim, 1843) (Coleoptera : Trogositidae), un prédateur commun des scolytes, est attiré par la phéromone de rassemblement d'I. paraconfusus. Ces résultats peuvent avoir d'importantes conséquences sur la mise au point d'un outil de gestion efficace de D. valens, basé sur les composés sémiochimiques. [Traduit par la Rédaction]
The impact of low temperatures in controlling the geographical distribution of plants

The distribution limits of three species, in the British Isles are discussed. For Verbena officinalis and Tilia cordata low temperatures are shown to influence distribution, by limiting the capacity either to flower or to fertilize ovules, respectively. In the case of Umbilicus rupestris , a long-term transplant population beyond the natural geographical limit of the species has evolved new low-temperature responses of seed germination and winter survival. The effect is a marked change of phenology, compared with populations of the species within its natural range, which enhances the capacity of the population to survive in a colder environment.