Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of deep sequencing exosome‐microRNA expression profile derived from CP‐II reveals potential role of gga‐miRNA‐451 in inflammation

Mycoplasma gallisepticum (MG) can cause chronic respiratory disease (CRD) in chickens. While several studies have reported the inflammatory functions of microRNAs during MG infection, the mechanism by which exosomal miRNAs regulate MG‐induced inflammation remains to be elucidated. The expression of exosome‐microRNA derived from MG‐infected chicken type II pneumocytes (CP‐II) was screened, and the target genes and function of differentially expressed miRNAs (DEGs) were predicted. To verify the role of exosomal gga‐miR‐451, Western blot, ELISA and RT‐qPCR were used in this study. The results showed that a total of 722 miRNAs were identified from the two exosomal small RNA (sRNA) libraries, and 30 miRNAs (9 up‐regulated and 21 down‐regulated) were significantly differentially expressed. The target miRNAs were significantly enriched in the treatment group, such as cell cycle, Toll‐like receptor signalling pathway and MAPK signalling pathway. The results have also confirmed that gga‐miR‐451‐absent exosomes derived from MG‐infected CP‐II cells increased inflammatory cytokine production in chicken fibroblast cells (DF‐1), and wild‐type CP‐II cell–derived exosomes displayed protective effects. Collectively, our work suggests that exosomes from MG‐infected CP‐II cells alter the dynamics of the DF‐1 cells, and may contribute to pathology of the MG infection via exosomal gga‐miR‐451 targeting YWHAZ involving in inflammation.
Oxytocin and positive couple interaction affect the perception of wound pain in everyday life

A large body of animal and human laboratory research has linked social interaction and support to pain perception, with a possible role for the neuropeptide oxytocin as a neuroendocrine mediator. However so far, it has been unclear whether these effects translate to ecologically valid everyday life behavior and pain perception. In a randomized placebo-controlled study, a standard suction blister skin wound was induced to N = 80 romantic couples (N = 160 individuals). Couples then received intranasal oxytocin or placebo twice daily and were either instructed to perform a positive social interaction (partner appraisal task, PAT) once in the laboratory and two times during the following five days, or not. During these days, all participants reported their subjective pain levels multiple times a day using ecologically momentary assessment. Results from hierarchical linear modeling suggest that pain levels within the couples were inter-related. In men, but not in women, oxytocin reduced pain levels. Women reported lower pain levels in the group of positive social interaction, while this effect did not show in men. These results suggest that intranasal oxytocin might have sex-specific effects with pain reducing effects in men but the opposite effects in women. In contrast, especially women benefit from positive interaction in terms of dampened pain levels after positive interaction. The results add to the evidence for health-beneficial effects of positive couple interaction and point to underlying neuroendocrine mechanisms in everyday life pain specifically. The sex-specific effects, in particular, may have implications for psychopharmacological treatment of pain in men and women.
Therapist effects vary significantly across psychological treatment care sectors.

Psychological intervention outcomes depend in part on the therapist who provides the intervention (a therapist effect). However, recent reviews suggest that therapist effects may vary as a function of the context in which care is provided, and therefore should not be generalised beyond that context. This study statistically analysed therapist effect differences between care sectors delivering psychological interventions. The sample comprised routine clinical data from 26,814 patients (69% female; mean age 38) and 466 therapists in five care sectors: primary care, secondary care, university, voluntary, and workplace. Therapist effects were analysed using multilevel models and Markov Chain Monte Carlo credible intervals. The therapist effect was significantly larger in primary care (8.4%) than in any other sector (1.1 - 2.3%) except secondary care (4.1%), after controlling for explanatory baseline and process variables as well as accounting for differences between clinics. There were no other significant differences detected between care sectors. These findings support the hypothesis that differences in effectiveness between therapists vary depending on the context in which psychological treatment is provided. Differences in relative therapist impact can vary by a factor of 4-8 across treatment sectors. This should be considered in the application of research evidence, treatment planning, and the design and delivery of psychological care provision.
Crimean-Congo Hemorrhagic Fever Virus Antibodies among Livestock on Corsica, France, 2014–2016

We conducted a serologic survey for Crimean-Congo hemorrhagic fever virus antibodies in livestock (cattle, sheep, and goats; N = 3,890) on Corsica (island of France) during 2014–2016. Overall, 9.1% of animals were seropositive, suggesting this virus circulates on Corsica. However, virus identification is needed to confirm these results.
Tuning the product selectivity of Cu hollow fiber gas diffusion electrode for efficient CO2 reduction to formate by controlled surface Sn electrodeposition.

Efficient CO2 electrochemical reduction reaction (CO2RR) relies not only on the development of selective/active catalysts but also on advanced electrode configuration to solve the critical issue of poor CO2 mass transport and derived sluggish cathodic reaction kinetics. In this work, to achieve a favorable reaction rate and product selectivity, we designed and synthesized an asymmetric porous Cu hollow fiber gas diffusion electrode (HFGDE) with controlled Sn surface electrodeposition. The HFGDE derived from the optimal Sn electrodeposition condition exhibited a formate faradaic efficiency (FE) of 78% and a current density of 88 mA/cm2 at -1.2 V versus reversible hydrogen electrode, which are more than two times higher than those from the pristine Cu HFGDE. The achieved performance outperformed most of other Sn-based GDEs, indicating the creation of sufficient contact among CO2, electrolyte and electrode catalyst through the design of hollow fiber pore structure and catalytic active sites. The enhancement of formate production selectivity and the suppression of hydrogen by-product was attributed to the optimized ratio of SnOx species on the electrode surface. The best performance was seen for the HFGDE with the highest Sn2+/Sn4+ (120s deposition), likely due to the modulating effect of Cu substrate via electron donation with Sn species. The selectivity control strategy developed in the asymmetric HFGDE provides an efficient and facile method to stimulate selective electrochemical reactions in which gas-phase reactant with low solubility is involved.
Interleukin‐33 alleviates psoriatic inflammation by suppressing the T helper type 17 immune response

Psoriasis is a chronic inflammatory skin disease with unclear pathogenesis. Interleukin‐33 (IL‐33) is highly expressed in patients with psoriasis, but its role in psoriasis is unknown. The aim of this study was to investigate the possible role of IL‐33 in the pathogenesis and treatment of psoriasis. IL‐33 expression was determined using enzyme‐linked immunosorbent assay, real‐time fluorescent quantitative polymerase chain reaction and immunohistochemical staining. CD4+ T cells were sorted using magnetic beads and treated with or without IL‐33. Imiquimod (IMQ) was used to induce psoriatic inflammation in mice. The frequency of immune cells was determined using flow cytometry. The cytokine level in mouse skin was measured using cytometric bead array. Our results showed that IL‐33 was highly expressed in the lesional skin and serum of patients with moderate‐to‐severe plaque psoriasis. IL‐33 inhibited the expression of IL‐17 in CD4+ T cells of psoriasis patients. Subcutaneous injection of IL‐33 alleviated the IMQ‐induced psoriatic inflammation in mice, reduced tumor necrosis factor‐α and IL‐23 expression, and decreased the proportion of T helper type 17 (Th17) cells in the skin‐draining lymph nodes in the mice. Our results suggest that IL‐33 plays a protective role in the pathogenesis of psoriasis by suppressing Th17 cell differentiation and function. The potential therapeutic effect of IL‐33 in treating psoriasis warrants further investigation.
Age and sex-specific reference ranges are needed for the Aldosterone/Renin Ratio.

OBJECTIVE Current Endocrine Society Clinical Practice Guidelines use a specific aldosterone/renin ratio (ARR) threshold to screen for primary aldosteronism (a treatable disease causing up to 15% of hypertension in primary care) in all patients. We sought to characterize demographic variations in the ARR, hypothesizing a need for age- and sex-specific reference ranges to improve the accuracy of the test. DESIGN Retrospective cross-sectional analysis of ARR measurements at a single tertiary hospital from December 2016 - June 2018. PATIENTS 442 patients with clinically-indicated ARR were included, after excluding those who were on spironolactone or the oral contraceptive pill, were pregnant, or had an existing adrenal condition. MEASUREMENTS Aldosterone, renin, and the ARR. RESULTS Among those aged 20-39 years (n=74), females had significantly higher median aldosterone (369 vs 244 pmol/L, p=0.028), lower median renin (17.0 vs 27.6 mIU/L, p=0.034), and higher median ARR (20.7 vs 10.3 (pmol/L)/(mIU/L), p=0.001) than males, despite having lower systolic (135 vs 145 mmHg, p=0.021) and diastolic (89 vs 96.5 mmHg, p=0.007) blood pressure. The ≥60 year age group (n=157) also had significant sex differences in the ARR. With increasing age (20-39 vs ≥60 years), there was a significant fall in plasma aldosterone in females (369 pmol/L vs 264 pmol/L, p=0.005), with no change observed in males. CONCLUSIONS For those 20-39 years old, aldosterone and the ARR are significantly higher in females despite a lower systolic and diastolic BP, highlighting the potential for false positive results. Our findings indicate the need for prospective studies with a control population to define age- and sex-specific ARR reference ranges.
Oocytes suppress FOXL2 expression in cumulus cells in mice †

Abstract Cumulus cells and mural granulosa cells (MGCs) play distinct roles during follicular development, and normal development of these cell lineages is critical for the female fertility. Transcriptomic diversification between the two cell lineages is obviously a critical mechanism for their functional diversification; however, the transcriptional regulators responsible for this event have not been fully defined. In this study, we sought to identify key transcriptional regulators responsible for the differential gene expression between the two cell lineages. In silico analysis of transcriptomic comparison between cumulus cells and MGCs identified several candidate regulators responsible for the diversification of the two cell lineages. Among them, we herein focused on forkhead box L2 (FOXL2) and showed that expressions of FOXL2 as well as its target transcripts were differentially regulated between cumulus cells and MGCs. The lower expression of FOXL2 in cumulus cells seemed to be due to the suppression by oocyte-derived paracrine signals. These results suggest that FOXL2 is one of the critical transcription factors that determine cumulus cell and MGC lineages under the control of oocytes. Summary sentence FOXL2 is one of the critical transcription factors that determine transcriptional diversification between cumulus and MGC lineages under the control of oocytes.
Predicting pathological complete response in rectal cancer after chemoradiotherapy with a random forest using 18F-fluorodeoxyglucose positron emission tomography and computed tomography radiomics

Background Neoadjuvant chemoradiotherapy (NCRT) followed by surgery is the standard treatment for patients with locally advanced rectal cancer. This study developed a random forest (RF) model to predict pathological complete response (pCR) based on radiomics derived from baseline 18F-fluorodeoxyglucose ([18F]FDG)-positron emission tomography (PET)/computed tomography (CT). Methods This study included 169 patients with newly diagnosed rectal cancer. All patients received 18F[FDG]-PET/CT, NCRT, and surgery. In total, 68 radiomic features were extracted from the metabolic tumor volume. The numbers of splits in a decision tree and trees in an RF were determined based on their effects on predictive performance. Receiver operating characteristic curve analysis was performed to evaluate predictive performance and ascertain the optimal threshold for maximizing prediction accuracy. Results After NCRT, 22 patients (13%) achieved pCR, and 42 features that could differentiate tumors with pCR were used to construct the RF model. Six decision trees and seven splits were suitable. Accordingly, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 81.8%, 97.3%, 81.8%, 97.3%, and 95.3%, respectively. Conclusions By using an RF, we determined that radiomics derived from baseline 18F[FDG]-PET/CT could accurately predict pCR in patients with rectal cancer. Highly accurate and predictive values can be achieved but should be externally validated.
Pengaruh Dukungan Spritual terhadap Tingkat Kecemasan pada Pasien Pre-Operasi

Anxiety is an emotional response to the subjective judgment of individuals, influenced by the unconscious and not specifically known to cause. Based on data from the World Health Organization (WHO) in 2007, the United States analyzed data from 35,539 surgical clients treated in the intensive care unit from 2003 to 2006, of which 2,473 clients (7%) experienced anxiety conditions. The results of Masood Jawaid's research, et al.(2006) found that average respondents in a state of moderate anxiety as much as 57.65. The purpose of this study was to determine the effect of spiritual support on anxiety levels in pre-operative patients. This study was Quasi-Experiment with one group pretest-posttest design, sampling was using purposive sampling technique. Sample amounted to 16 respondents, population amounted to 325 patients. The Data collection tool was using a questionnaire sheet. The statistical test was using Wilcoxon test. The average yield of anxiety prior to therapy spiritual support was 49.88, with a standard deviation of 6.449. While the average anxiety after the therapy spiritual support was 46.81 with a standard deviation of 6.002. The statistical test result showed that p-value = 0.001, there was an effect of a spiritual support therapeutic to the level of anxiety of patients pre-operation at Imanuel Hospital Lampung Year 2017. This study suggested therapy for spiritual support as nursing care in patients who experience anxiety.
Genetic variants associated with glycaemic response to treatment with dipeptidylpeptidase 4 inhibitors.

Aim: We examined associations of eight SNPs in/near seven candidate genes with glycaemic response to 6 month treatment with DPP4 inhibitors. Patients & methods: 206 patients with type 2 diabetes (116 men and 90 women) were treated with sitagliptin or vildagliptin (both 100 mg/day) in combination with metformin or metformin/sulphonylurea over 6 months, and the reduction in glycated haemoglobin (HbA1c) was measured. Results: Rs6923761 in GLP1R was significantly associated with a reduction in HbA1c (adjusted p = 0.006). Homozygotes for the minor A allele had smaller reduction in HbA1c by 0.4% (4 mmol/mol) than the G allele carriers (p = 0.016). Conclusion: The missense variant rs6923761 in the GLP1R gene was associated with a smaller glycaemic response to 6 month gliptin therapy in diabetic patients of Central European origin.
Rape Myth Acceptance of Police Officers in Portugal

This study intended to examine rape myth acceptance (RMA) among police officers and its relationship with sociodemographic data, length of service, specific training in the field, and professional experience with victims of rape. To this end, we applied the Sexual Violence Beliefs Scale (ECVS) and controlled for sociodemographic data, as well as professional experience and specific training in the field, through a self-report questionnaire. The sample was composed of 400 police officers from a city in the north of Portugal, aged between 29 and 54 years, and most were men (94.3%). We found that tolerance to overall sexual violence exhibits positive correlations with age and length of service, as well as negative correlations with education levels. Differences were also found regarding gender, with men exhibiting greater tolerance/acceptance of overall sexual violence. It was also found that officers who exhibit higher tolerance/acceptance for sexual violence, overall, are those who do not consider it relevant to receive specific training in the field of sexual violence to perform their duties, who report not having any professional experience with cases of sexual violence and also do not consider it necessary to have specific abilities for these types of cases. Implications for decision-making in legal proceedings are discussed, stressing the need for specific specialization in intervention with victims of sexual assault, with a strong practical component.
Mild to moderate partial sleep deprivation is associated with increased impulsivity and decreased positive affect in young adults

Abstract The effects of mild–moderate partial sleep deprivation on affective and cognitive functioning were evaluated in a naturalistic home environment, mimicking short sleep typically caused by demands from work or society. A total of 52 healthy individuals aged 18–35 was included in an 11-day study protocol. Participants slept at home, and sleep patterns were observed using actigraphs and sleep diaries. After maintaining habitual sleep for 7 days, the participants were asked to sleep 2 hours less than their average sleep duration for the last three nights of the study protocol. A not-X continuous performance test was administered at 9 am (± 90 minutes) on days 1, 4, 8 (habitual sleep), 9 and 11 (sleep deprivation). Performance-based measures included response accuracy and speed. Participant-reported measures included how well the participants felt they performed and how exhausted they were from taking the test, as well as positive and negative affect. There was a significant change in reaction time, number of commission errors, subjective performance, subjective exertion, and positive affect across the visits. Specifically, there was a linear decrease in reaction time, performance, and positive affect throughout the study, and a significant quadratic trend for commissions and exertion (first decreasing, then increasing after sleep deprivation). The univariate tests for omissions and negative affect were not significant. We conclude that sleeping 1.5–2 hours less than usual leads to faster response speed, but more commission errors and decreased positive affect. This indicates that individuals become more impulsive and experience less positive affect after a period of short sleep.
Effect of Cerium Oxide Nanoparticles on Oxidative Stress Biomarkers in Rats’ Kidney, Lung, and Serum

Background: The present study aimed to evaluate the effects of different concentrations of CONPs on the OS status in kidney, lung, and serum of rats. Methods: Male Wistar Rats were treated intraperitoneally with 15, 30, and 60 mg/kg/day of CONPs. The biochemical parameters, including TAC, TTG, MDA, SOD, and CAT were assayed in serum, kidney, and lung tissues. Results: MDA decreased, but TTG and CAT increased in serum by the administration of CONPs at 15 mg/kg. In kidney homogenate obtained from the group treated with CONPs at 15 mg/kg, TAC, TTG, and CAT significantly increased compared to the control group. However, CONPs at 15, 30, and 60 mg/kg significantly decreased MDA level compared to the control group. In lung tissue, CONPs in doses of 15, 30 and 60 mg/kg significantly decreased CAT activity, TTG and TAC compared to the control group, while in kidney tissue, CONPs at the concentrations of 30 and 60 mg/kg significantly increased MDA compared to the control group. Conclusion: Our findings suggest that CONPs attenuate OS in the kidney and affect the serum levels of OS-related markers but induce OS in the lung tissue in a dose-dependent manner.
Political depression? A big-data, multimethod investigation of Americans' emotional response to the Trump presidency.

Previous studies suggested that the 2016 presidential elections gave rise to pathological levels of election-related distress in liberal Americans; however, it has also been suggested that the public discourse and the professional discourse have increasingly overgeneralized concepts of trauma and psychopathology. In light of this, in the current research, we utilized an array of big data measures and asked whether a political loss in a participatory democracy can indeed lead to psychopathology. We observed that liberals report being more depressed when asked directly about the effects of the election; however, more indirect measures show a short-lived or nonexistent effect. We examined self-report measures of clinical depression with and without a reference to the election (Studies 1A & 1B), analyzed Twitter discourse and measured users' levels of depression using a machine-learning-based model (Study 2), conducted time-series analysis of depression-related search behavior on Google (Study 3), examined the proportion of antidepressants consumption in Medicaid data (Study 4), and analyzed daily surveys of hundreds of thousands of Americans (Study 5), and saw that at the aggregate level, empirical data reject the accounts of "Trump Depression. " We discuss possible interpretations of the discrepancies between the direct and indirect measures. The current investigation demonstrates how big-data sources can provide an unprecedented view of the psychological consequences of political events and sheds light on the complex relationship between the political and the personal spheres. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Clinical and clinicopathological features and outcomes of Miniature Dachshunds with bone marrow disorders

Non-neoplastic bone marrow disorders such as non-regenerative immune-mediated anemia, pure red cell aplasia, and myelodysplastic syndrome are major causes of non-regenerative anemia in dogs. However, there has been no study on the clinical and clinicopathological features of canine non-neoplastic bone marrow disorders in Japan. Hence, we first investigated the breed disposition of non-neoplastic bone marrow disorders that induce anemia as a retrospective study and found that Miniature Dachshund (MD) was a predisposed breed. Based on this finding, we investigated the clinical and clinicopathological features of non-neoplastic bone marrow disorders in MDs as a preliminary retrospective study, and we compared them between immunosuppressive treatment-responsive and -resistant MDs. We found that treatment-resistant MDs showed thrombocytosis and increased frequencies of dysplastic features in the peripheral blood. These results indicate that bone marrow disorders in treatment-resistant MDs might manifest distinct features compared with those in treatment-sensitive MDs, and sensitivity to immunosuppressive treatments could be predicted based on thrombocytosis and dysplastic features in the peripheral blood. Further studies that examine aberrations in the genome are needed to elucidate the pathophysiology of bone marrow disorders in MDs.