Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis

Introduction: The aim of this study was to evaluate the effectiveness of chlorhexidine (CHX), sodium hypochlorite (NaOCl), calcium hydroxide (CH) and double antibiotic paste (DAP) mixed with silver nanoparticles (AgNPs) against Enterococcus faecalis. Methods and materials: Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm formation inhibition (after 72 h) of the experimental substances alone or mixed with AgNPs were measured against E. faecalis using microtiter plate method. Bacterial cultures turbidity was measured using a spectrophotometer. All procedures were performed in triplicates. Results: The MIC values for CHX, NaOCl, CH and DAP were equal to 0.012, 1.25, 1.6 and 0.156 mg/mL, and their MBC’s were 0.025, 2.5, 0 and 0.625 mg/mL. After mixing them with AgNPs, the MIC’s for CHX, NaOCl, CH and DAP were reduced to 0.0032, 0.158, 0.2 and 0.0391 mg/mL, while their MBC’s were reduced to 0.0064, 0.0632, 0.401 and 0.0156 mg/mL. Biofilm formation inhibition occurred in higher dilutions of all irrigants and medicaments as they were mixed with Ag NPs. Conclusions: Adding AgNPs resulted in an increased antimicrobial activity at the tested dilutions for all experimental substances. More investigations in in vivo conditions are required to confirm the results of this study.
Effects of riboflavin and AQS as electron shuttles on U(vi) reduction and precipitation by Shewanella putrefaciens

Understanding the mechanisms for electron shuttles (ESs) in microbial extracellular electron transfer (EET) is important in biogeochemical cycles, bioremediation applications, as well as bioenergy strategies. In this work, we adapted electrochemical techniques to probe electrochemically active and redox-active Shewanella putrefaciens. This approach detected flavins and humic-like substances of Shewanella putrefaciens, which were involved in electron transfer to the electrode. A combination of three-dimensional excitation-emission (EEM) florescence spectroscopy methods identified a mixture of riboflavin and humic-like substances in supernatants during sustained incubations. The reductive behaviour of U(vi) by Shewanella putrefaciens in the presence of riboflavin (RF) and anthraquinone-2-sulfonate (AQS) was also investigated in this study. The results indicated that RF and AQS significantly accelerated electron transfer from cells to U(vi), thus enhancing reductive U(vi). The precipitate was further evidenced by SEM, FTIR, XPS and XRD, which demonstrated that chernikovite [H2(UO2)2(PO4)2·8H2O] became the main product on the cell surface of S. putrefaciens. In a contrast, U(iv) mainly existed amorphously on the cell surface of S. putrefaciens with added RF and AQS. This work has significant implications in elucidating RF and AQS as electron shuttles that are efficient in reduction of uranium in geological environments.
Comparison of cooled and conventional radiofrequency applications for the treatment of osteoarthritic knee pain

Background and Aims: Osteoarthritis is a progressive degenerative joint disease that affects the joint cartilage and surrounding tissues. It has been determined that osteoarthritis-induced knee pain is the most common cause of physical disability in the elderly. Material and Methods: In this study, the genicular nerve RF treatments of patients with osteoarthritic knee pain conducted at the Sakarya University Training and Research Hospital in the algology clinic of the Anaesthesiology and Reanimation Department between January 2016 and December 2016 were retrospectively examined. The preoperative and postoperative 2nd, 6th, and 12th week visual analog scale (VAS) and Turkish validated Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores were recorded. In addition, any complications after the treatment and side effects (bleeding, neurological damage, infection, etc.) were recorded in the file. Results: When the data of the patients were evaluated statistically, the preoperative VAS and WOMAC scores were found significantly decreased compared with the postoperative 2nd week, 6th week, and 12th week scores in patients who applied both conventional radiofrequency (RF) and cooled RF. However, there was no statistically significant difference between the two techniques. Conclusion: We found that both cooled and conventional RF techniques in genicular nerve ablation are similarly effective in reducing pain in patients with osteoarthritis-induced knee pain and improving patients' physical functions. The complication rates are very low and there was no superiority to each other.
Cardiovascular Disease Risk Factors and 10 Year Risk of Cardiovascular Events among Women over the Age of 40 Years in an Urban Underprivileged Area of Bangalore City

Background: Cardiovascular disease (CVD) is one of the leading causes of death among Indian women but not a focus of current strategies to improve women's health. Objectives: To assess 10-year CVD risk and estimate the prevalence of CVD risk factors among underprivileged women. Methods: A cross-sectional study among women aged 40–79 years in an urban underprivileged area of Bangalore city, using nonlaboratory-based World Health Organization and the International Society of Hypertension risk prediction chart and INTERHEART list of CVD risk factors. Blood pressure, random blood sugar, height, weight, and waist circumference were measured. The Chi-square test was done for the association between CVD risk status and risk factors. Multiple logistic regression performed to calculate adjusted odds ratios (ORs). Results: Of 1027 women, 11% women had high risk of CVD (≥20% risk of fatal/nonfatal cardiovascular event within next 10 years). The prevalence of CVD risk factors was high with 20.2% diabetes, 53.7% hypertension, 77% overweight/obesity, and 92% central obesity. Majority were physically inactive with unhealthy diet, lacking daily fruits and vegetables, excess consumption of salty food, junk food, red meat, and excess oil. Significantly higher CVD risk found among women who were sedentary during leisure time (P = 0.028), single/separated/widowed women (OR = 1.84 [1.26–4.29] P = 0.002), and women who did not walk or cycle as a regular means of transport (OR = 1.47 [1.98–2.19] P = 0.041). Conclusion: High risk of CVD among underprivileged women reveals an urgent need for community-based interventions for prevention of CVD. Screening and management of diabetes and hypertension must include weight reduction and health education strategies. Policy-makers need to initiate efforts toward improving affordability of healthy diets for the urban underprivileged.
Retrograde Autologous Priming Method Reduces Plasma Free Hemoglobin Level in Aortic Surgery

Background: Although conventional cardiopulmonary bypass (cCPB) is still the most widely used method in open heart surgery, methods such as retrograde autologous priming (RAP) are increasingly popular in terms of limiting hemodilution. Our hypothesis is that the use of the RAP method in aortic surgery may result in a limitation of hemodilution and a decrease in fHb levels. For this purpose, plasma free hemoglobin (fHb) levels were investigated in adult open aortic arch repair with axillary artery cannulation patients using cCPB and rRAP methods. Materials and Methods: In this study, a total of 36 patients undergoing aortic surgery using rRAP and standard cCPB were investigated. Measurements were performed at five time points: After induction of anesthesia, 5th minute of CPB, 10th minute of antegrade cerebral perfusion, 30th minute after declamping of aorta, and at sternum closure. Besides hemodynamic variables, arterial blood gas analysis and postoperative variables, patients were assessed for fHb levels. Results: The rRAP group had a significantly lower increase in fHb levels in T3, T4, and T5 time points, when compared to the cCPB group (p = 0.002, 0.047, 0.009, respectively). There was no significant difference between the rRAP and cCPB groups in other intraoperative, and postoperative variables. Also, it was observed that rRAP did not make a difference in terms of blood and blood product transfusion. Conclusion: In this study, in patients undergoing aortic surgery, a reduction in the increase of fHb was observed with the rRAP method which is a simple procedure that does not require high cost or advanced technology.
Postoperative Atrial Fibrillation in Coronary Artery Bypass Grafting Herald Poor Outcome

Introduction: Atrial fibrillation in postoperative period is common. Although the event of atrial fibrillation is associated with reduced cardiac output and its consequences and cerebrovascular events, its effect on outcome is not clearly documented. This study is done to evaluate the effect of atrial fibrillation on outcome of the operation. Materials and Methods: This is a retrospective case-control study. A total of 263 patients received coronary artery bypass grafting during this period. The data for demographics, comorbidities, preoperative medications, operative details, and echocardiographic parameters of left ventricular functions were acquired. A total of 24 patients had atrial fibrillation (Group I), while 239 remained in normal sinus rhythm (Group II). The outcome is measured as combined of death and postoperative myocardial infarction (MI). Results: The groups are comparable in demographic, preoperative medications, operative, and left ventricular parameters. Of the 24 (9.12%) patients who had postoperative atrial fibrillation, 11 were discharged on medical management. Nine patients reverted to sinus rhythm at discharge. Atrial fibrillation persisted in 8 patients 1 week after discharge and 3 patients after 1 month. The requirement of intraaortic balloon pump (IABP) was statistically significant in group I (5 in group I vs. 10 in group II, n = 0.001). There were 4 deaths in group 1 and 7 in group 2 (P = 0.002), however, the combined end point was achieved in 4 (16.6%) vs. 22 (9.2%), respectively, P = 0.1. Conclusion: The appearance of atrial fibrillation heralds increased requirement of IABP, MI, and death in patients undergoing coronary artery bypass grafting.
Optimum time of LMA ProSeal removal in adult patients undergoing isoflurane anesthesia: A randomized controlled trial

Background and Aims: Optimum timing of laryngeal mask airway (LMA) removal after general anesthesia with isoflurane is debatable. The objective was to investigate the potential benefits of removing LMA ProSeal at ≤0.4 Minimum alveolar concentration (MAC) isoflurane over awake and “deep plane” extubation after short duration laparoscopic gynecological surgery. Material and Methods: In this prospective randomized trial 90 adult female patients undergoing elective laparoscopic surgery under general anesthesia using LMA ProSeal™ as airway device were included. At the end of surgery, LMA ProSeal™ was removed when the patient was awake, could open mouth following verbal command (Group A); at MAC ≤0.4 (Group B); or at MAC of 0.6 (Group C). Adverse airway events like nausea, vomiting, airway obstruction, coughing, bucking, laryngospasm were noted. Statistical analyses were done by SPSS statistical software (IBM SPSS Statistics for Mac OS X, Version 21.0. IBM Corp, Armonk, NY). Results: Baseline demographic characteristics were comparable in all three groups. Coughing or bucking at the time of LMA removal was higher in group A (P = 0.004). Snoring and airway obstruction after LMA removal was significantly higher in group C compared to group A and group B (P = 0.002 and P = 0.011, respectively). There was significant change in mean arterial pressure and heart rate between before and after LMA removal on group A (P = 0.008 and P < 0.001, respectively) but not in other groups. Conclusion: MAC ≤0.4 can be considered optimum depth of anesthesia for removal of LMA Proseal in adult patients undergoing isoflurane anesthesia.
Artemether-loaded nanostructured lipid carriers: preparation, characterization, and evaluation of in vitro effect on Leishmania major

Background and purpose: Cutaneous leishmaniasis is a global health problem. The discovery of new and highly efficient anti-leishmanial treatments with lower toxicity is globally needed. The current study was carried out to evaluate the anti-leishmanial effects of artemether (ART) and ART-loaded nanostructured lipid carriers (ART-NLCs) against promastigotes and amastigotes of Leishmania major. Experimental approach: Solvent diffusion evaporation technique was applied to prepare ART-NLCs. These nanoparticles were characterized using a particle size analyzer (PSA), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The antiparasitic activity on amastigote was assessed in J774 cell culture. The drug cytotoxicity on promastigote and macrophage was assessed using the MTT technique after 24 and 48 h and compared with NLCs, ART, and amphotericin B, as the control agents. The selectivity index was calculated for the agents. Findings/Results: The DLS and PSA techniques confirmed that ART-NLCs were homogenous in size with an average diameter of 101 ± 2.0 nm and span index of 0.9. The ART-NLCs significantly heighten the anti-leishmanial activity of ART (P < 0.001). The IC50 values of ART and ART-NLCs on promastigotes after 24 and 48 h were 76.08, 36.71 and 35.14, 14.81 μg/mL, respectively while they were calculated 53.97, 25.43 and 20.13, 11.92 for amastigotes. Also, ART-NLCs had the lowest cytotoxicity against macrophages. Furthermore, among the agents tested, ART-NLCs had the highest selectivity index. Conclusion and implications: ART-NLCs had lower cytotoxic effects than ART and amphotericin B, also its selectivity index was significantly higher. Based on the findings of the study, this formulation could be a promising candidate for further research into leishmaniasis treatment.
Evaluating the adhesion properties of crude oil fractions on mineral aggregates at different temperatures through reactive molecular dynamics

Abstract Understanding the structure-property relationships of the binder-aggregate interface at the molecular level provides insights into the nature of their adhesion and could pave the way for better asphalt mixtures. In this study, molecular dynamic simulation and reactive force field (REAXFF) were employed to investigate the adhesion properties of Saturate, Asphaltene, Resin and Aromatic (SARA) components of asphalt binder on silicon dioxide (SiO2) aggregates at different temperatures (–12160). The result showed that Aromatic and Resin had their maximum adhesion properties around 80, while Saturate had the minimum work of adhesion. Also, due to hydrogen bonding and covalent bond breaking, the adhesion properties of Asphaltene molecule was 300% higher than the other components.
ASSESSMENT OF FIREFIGHTING EFFICIENCY OF VEGETATION FIRES

Assessment of firefighting efficiency of vegetation fires in Curitiba-Paraná. Vegetation fires, when not controlled, cause economic disruption, temporary loss of vegetation, and damage to soil, fauna and health. To improve the process of fire prevention and firefighting, it is necessary to evaluate the performance of the involved agents. The objective of this study was to evaluate the firefighting efficiency of vegetation fires in the municipality of Curitiba, Paraná, from 2011 to 2015, using records from the Fire Department of the Military Police of Paraná. Once the consistency of the fire records was verified, they were classified and information was gathered regarding the extent of burnt area, time of first attack, combat time, main fire-extinguishing methods used, and amount of water used. The results indicate that 88% of the records registered a burnt area inferior or equal to four hectares. In addition, the mean burnt area was of 2,399.21 m2, the mean attack time was of 14.1 minutes, and the mean combat time was of 29.9 minutes, all lower than the ones presented by studies from different locations. As for the fire-extinguishing methods, it was verified that smothering equipment and water were used in 66.4 and 60.6% of the records, respectively. The mean amount of water used was of 1,186.56 liters per fire, indicating a minimum volume necessary for water storage containers for firefighting in the study area. Based on the results, we concluded that the firefighting of vegetation fires in Curitiba is efficient.
OP-BRAI180285 3428..3442

Mutations in the endosome-associated protein CHMP2B cause frontotemporal dementia and lead to lysosomal storage pathology in neurons. We here report that physiological levels of mutant CHMP2B causes reduced numbers and significantly impaired trafficking of endolysosomes within neuronal dendrites, accompanied by increased dendritic branching. Mechanistically, this is due to the stable incorporation of mutant CHMP2B onto neuronal endolysosomes, which we show renders them unable to traffic within dendrites. This defect is due to the inability of mutant CHMP2B to recruit the ATPase VPS4, which is required for release of CHMP2B from endosomal membranes. Strikingly, both impaired trafficking and the increased dendritic branching were rescued by treatment with antisense oligonucleotides targeting the well validated frontotemporal dementia risk factor TMEM106B, which encodes an endolysosomal protein. This indicates that reducing TMEM106B levels can restore endosomal health in frontotemporal dementia. As TMEM106B is a risk factor for frontotemporal dementia caused by both C9orf72 and progranulin mutations, and antisense oligonucleotides are showing promise as therapeutics for neurodegenerative diseases, our data suggests a potential new strategy for treating the wide range of frontotemporal dementias associated with endolysosomal dysfunction.
OP-ICES180187 410..417

Potential for managing life history diversity in a commercially exploited intermediate predator, the goldsinny wrasse (Ctenolabrus rupestris) Esben Moland Olsen *, Kim Tallaksen Halvorsen, Torkel Larsen, and Anna Kuparinen Institute of Marine Research Flødevigen, Nye Flødevigvei 20, 4817 His, Norway Department of Natural Sciences, Centre for Coastal Research (CCR), University of Agder, P.O. Box 422, 4604 Kristiansand, Norway Department of Biosciences, Centre for Ecological and Evolutionary Synthesis (CEES), University of Oslo, P.O. Box 1066, Blindern, 0316 Oslo, Norway Department of Biological and Environmental Science, P.O. Box 35, 40015 University of Jyväskylä, Finland *Corresponding author: tel: þ47 94288505; e-mail: esben.moland. olsen@imr.no. Olsen, E. M. , Halvorsen, K. T. , Larsen, T. , and Kuparinen, A. Potential for managing life history diversity in a commercially exploited intermediate predator, the goldsinny wrasse (Ctenolabrus rupestris). – ICES Journal of Marine Science, 76: 410–417.
OP-EUJC180393 948..955

OBJECTIVES: Neutrophil to lymphocyte ratio (NLR) has been shown to be a promising biomarker in several cancers. Prognostic biomarkers are still needed to define good candidates for lung metastasectomy for colorectal cancer. We aimed to evaluate the role of NLR. METHODS: Data from 574 patients who underwent lung metastasectomy for colorectal cancer in 3 departments of thoracic surgery from 2004 to 2014 were retrospectively reviewed. Overall survival (OS) and the time to pulmonary recurrence (TTPR) were the main end points. VC The Author(s) 2018. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. European Journal of Cardio-Thoracic Surgery 55 (2019) 948–955 ORIGINAL ARTICLE doi:10.1093/ejcts/ezy388 Advance Access publication 1 December 2018 D ow naded rom http/academ ic.p.com /ejcts/arti/5/948/5224506 by gest on 24 O cber 2021 RESULTS: Correlations between NLR and OS (R = 0.53), and NLR and TTPR (R = 0.389) were significant (P < 0.0001 for both), with corresponding Pearson R of -0.728 (P < 0.0001) and -0.624 (P < 0.0001), respectively. A receiver operating characteristic curve analysis highlighted an NLR cut-off value of 4.05 as the best predictor of OS and TTPR. NLR <_4.05 was observed in 238 patients (41.4%). In the univariable analysis, the median OS was 117 months for patients with NLR <_4.05 and decreased to 40 months for patients with NLR >4.05 (P < 0.0001). The median TTPR reached 52 months in case of NLR <_4.05 and decreased to 12 months in patients with NLR >4.05. In the multivariable analysis, NLR <_4.05 remained an independent favourable prognostic factor on both OS [hazard ratio [HR] 0.29, 95% confidence interval (CI) 0.167–0.503; P < 0.0001] and TTPR (HR 0.346, 95% CI 0.221–0.54; P < 0.0001). Significant correlations between NLR >4.05 and KRAS (Cramer’s V = 0.241, P < 0.0001) and BRAF (Cramer’s V = 0.153, P = 0.003) mutations were observed. CONCLUSIONS: NLR is a simple and powerful predictor of outcomes in patients undergoing pulmonary metastasectomy for colorectal cancer.
Implementation of code of practices (CoP) in selected poultry farms of Bangladesh

The overuse of antibiotics, chemicals and as well as lack of farm biosecurity and good hygiene practices in poultry production are considered to be contributors for occurring foodborne illness and many significant public health threats reported in both developed and developing countries nowadays. Considering the above, a piloted food safety activity was implemented jointly by the Department of Livestock Services and FAO-Food Safety program, Bangladesh in twenty-five selected poultry (broiler) dense subdistricts of the country. Good Agriculture Practices (GAP) and Good Hygiene Practices (GHP) related five (microbial) plus five (chemical) Code of Practices (CoP) were adopted at the farm level (N=500) through farmers’ motivation and intensive participatory training program. It was found that average production cost reduces, the feed conversion ratio decreases and mortality rate decreases that convince increase farm profitability in the best-practiced farms (n=81). In conclusion, Good Agriculture Practices (GAP) and Good Hygiene Practices (GHP) through certifying key control measures can increase profitability in broiler farming and CoP adapted farms found to be less public health hazardous than non-CoP farms in light of food safety and public health grounds. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 45-55
Role of DTAB and SDS in Bubble-Particle Attachment: AFM Force Measurement, Attachment Behaviour Visualization, and Contact Angle Study

Atomic force microscopy (AFM) and contact angle measurements were used to study the role of dodecyltrimethylammonium bromide (DTAB) and sodium dodecyl sulphate (SDS) in bubble-particle attachment. The results show that the forces between bubbles and the hydrophilic glass particle were always repulsive in the absence of DTAB and SDS. An attractive hydrophobic force was induced when the particles became hydrophobic, and the force was proportional to the water contact-angle. In the presence of DTAB and SDS, the cationic head group of DTAB adsorbed onto the negative hydrophilic glass surface as a monolayer and thus induced a hydrophobic force. However, at a high DTAB concentration, the DTAB molecules began to adsorb as a bilayer, reverting back to a hydrophilic surface. The hydrophobic force disappeared and the water film between the bubble and particle was stabilised under the repulsive double-layer force. The anionic SDS molecules could not adsorb onto the hydrophilic glass surface. The repulsive force always dominated the bubble-particle interaction. In the case of hydrophobic glass, the hydrophobic force decreased, and even disappeared, with the addition of DTAB and SDS. All the findings from the AFM force curves were consistent with the attachment behaviour and contact angle results.