Miracle-dz/UniFAR-FTUs huggingface dataset:
Exogenous Calcium Delays Grape Berry Maturation in the White cv. Loureiro While Increasing Fruit Firmness and Flavonol Content

Vineyard calcium (Ca) sprays have been increasingly used by grape growers to improve fruit firmness and thus maintain quality, particularly in periods of heavy rains and hail. The observation that Ca visibly modified berry size, texture, and color in the most prominent white cultivar of the DOC region ‘Vinhos Verdes’, cultivar (cv.) Loureiro, led us to hypothesize that Ca induced metabolic rearrangements that resulted in a substantial delay in fruit maturation. Targeted metabolomics by ultra-performance liquid chromatography coupled to mass spectrometry and directed transcriptomics were thus combined to characterize the metabolic and transcriptional profiles of cv. Loureiro berries that, together with firmness, °Brix, and fruit weight measurements, allowed to obtain an integrated picture of the biochemical and structural effects of Ca in this cultivar. Results showed that exogenous Ca decreased amino acid levels in ripe berries while upregulating PAL1 expression, and stimulated the accumulation of caftaric, coutaric, and fertaric acids. An increase in the levels of specific stilbenoids, namely E-piceid and E-ω-viniferin, was observed, which correlated with the upregulation of STS expression. Trace amounts of anthocyanins were detected in berries of this white cultivar, but Ca treatment further inhibited their accumulation. The increased berry flavonol content upon Ca treatment confirmed that Ca delays the maturation process, which was further supported by an increase in fruit firmness and decrease in weight and °Brix at harvest. This newly reported effect may be specific to white cultivars, a topic that deserves further investigation.
Identification of Modified and Unmodified Lipophilic B-Diketones Using Electrospray Ionisation Mass Spectrum (Esi-Ms)

Electrospray ionization mass spectroscopic technique (ESIMS) has already been established as a powerful tool in the characterization of small and large molecules [1]. ESI concept was firstly introduced in 1968 by Dole [2]. It is a less energetic ionization technique, where a sample is protonated in gas phase to form cationic species; not radical (as is the case of impact ionization approach), [3] thus causing little or no fragmentation of the parent compound. It is pertinent to make do with ESI-MS because, in some cases, the column in GC is not suitable for detecting very high molecular weight organic compounds. Literature report has it that, ESI-MS method is reliable, robust and much less time consuming than the GC methods [4]. Importantly, ESI-MS is simple, fast, accurate, sensitive and straightforward technique for the analyzing compounds [5,6]. Alternative techniques for analyses of compounds such as NMR need further processing and requisite expertise to enable the identification of a compound.
On Using Languages Other Than the Target One in L2 Adult Language Education: Teachers’ Views and Practices in Modern Greek Classrooms

Current developments in language education call for a reassessment of the role that students’ already-established linguistic repertoires can play in language teaching. This study probed into adult second language education in Modern Greek offered in Greece, where classes are culturally and linguistically diverse. We investigated teachers’ views and perceived practices regarding the use of other languages in their classes. A mixed-method design was followed. Data on teachers’ opinions was collected via a questionnaire completed by 30 teachers. Complementary data on teachers’ practices collected through observations of two classes was also studied. The results indicated that English was mainly used by the teachers as a mediation language, although a wide variation was reported in the amount of other-language use. Large variations were also reported in the students’ behaviour. Teachers stressed several benefits from using other languages in class, but also expressed concerns about excessive reliance on other languages and on how using a support language would impact students with limited proficiency in this language. These findings were discussed in light of recent developments in language education and implications for teacher training were considered.
Sp1-Induced lncRNA Rmrp Promotes Mesangial Cell Proliferation and Fibrosis in Diabetic Nephropathy by Modulating the miR-1a-3p/JunD Pathway

Diabetic nephropathy (DN) is a serious complication of diabetes mellitus. Long non-coding RNAs (lncRNAs) are regulators in DN progression. However, the regulatory mechanisms of multiple lncRNAs in DN remain to be determined. Our aim was to investigate the function and molecular mechanism of lncRNA RNA component of mitochondrial RNAase P (Rmrp) in DN. Here, we observed that the expression of Rmrp was up-regulated in the kidney of db/db DN mice and high glucose induced glomerular mesangial cells (MC). More importantly, the abnormal transcription of Rmrp was induced by nuclear transcription factor Sp1, which promotes the proliferation and production of fibrotic markers in MC. Subsequently, we screened the miRNAs related to Rmrp and found that Rmrp and miR-1a-3p are co-localized at the subcellular level of MC, and Rmrp could directly binds to miR-1a-3p. Further mechanism research demonstrated that the elevated miR-1a-3p significantly attenuated the proliferation and fibrosis-promoting effects induced by up-regulation of Rmrp. At the same time, we also investigated that miR-1a-3p can directly bind to Jun D proto-oncogene (JunD), thereby regulating the protein level of JunD. Rmrp-induced proliferation and fibrogenesis were reversed by co-transfection with JunD siRNA. In summary, Sp1 induced lncRNA Rmrp could drive the expression of JunD via sponging miR-1a-3p in DN progression.
DeepConsensus: Gap-Aware Sequence Transformers for Sequence Correction

Pacific BioScience (PacBio) circular consensus sequencing (CCS) generates long (10-25 kb), accurate “HiFi” reads by combining serial observations of a DNA molecule into a consensus sequence. The standard approach to consensus generation uses a hidden Markov model (pbccs). Here, we introduce DeepConsensus, which uses a unique alignment-based loss to train a gap-aware transformer-encoder (GATE) for sequence correction. Compared to pbccs, DeepConsensus reduces read errors in the same dataset by 42%. This increases the yield of PacBio HiFi reads at Q20 by 9%, at Q30 by 27%, and at Q40 by 90%. With two SMRT Cells of HG003, reads from DeepConsensus improve hifiasm assembly contiguity (NG50 4.9Mb to 17.2Mb), increase gene completeness (94% to 97%), reduce false gene duplication rate (1.1% to 0.5%), improve assembly base accuracy (Q43 to Q45), and also reduce variant calling errors by 24%.
Fecal Microbiota Transplant Mitigates Adverse Outcomes Seen in Patients Colonized With Multidrug-Resistant Organisms Undergoing Allogeneic Hematopoietic Cell Transplantation

The gut microbiome can be adversely affected by chemotherapy and antibiotics prior to hematopoietic cell transplantation (HCT). This affects graft success and increases susceptibility to multidrug-resistant organism (MDRO) colonization and infection. We performed an initial retrospective analysis of our use of fecal microbiota transplantation (FMT) from healthy donors as therapy for MDRO-colonized patients with hematological malignancy. FMT was performed on eight MDRO-colonized patients pre-HCT (FMT-MDRO group), and outcomes compared with 11 MDRO colonized HCT patients from the same period. At 12 months, survival was significantly higher in the FMT-MDRO group (70% versus 36% p = 0.044). Post-HCT, fewer FMT-MDRO patients required intensive care (0% versus 46%, P = 0.045) or experienced fever (0.29 versus 0.11 days, P = 0.027). Intestinal MDRO decolonization occurred in 25% of FMT-MDRO patients versus 11% non-FMT MDRO patients. Despite the significant differences and statistically comparable patient/transplant characteristics, as the sample size was small, a matched-pair analysis between both groups to non-MDRO colonized control cohorts (2:1 matching) was performed. At 12 months, the MDRO group who did not have an FMT had significantly lower survival (36.4% versus 61.9% respectively, p=0.012), and higher non relapse mortality (NRM; 60.2% versus 16.7% respectively, p=0.009) than their paired non-MDRO-colonized cohort. Conversely, there was no difference in survival (70% versus 43.4%, p=0.14) or NRM (12.5% versus 31.2% respectively, p=0.24) between the FMT-MDRO group and their paired non-MDRO cohort. Collectively, these data suggest that negative clinical outcomes, including mortality associated with MDRO colonization, may be ameliorated by pre-HCT FMT, even in the absence of intestinal MDRO decolonization. Further work is needed to explore this observed benefit.
The Effect of Adverse Selection and Psychological Capital on Decision Making Involving Escalation Situations: An Experimental Study

This study examines the determinant of structural variable (adverse selection) and psychological variable (psychological capital – PsyCap) on manager’s tendency to escalate their commitment. This study used 2 x 2 factorial experimental design as a research method. The participant of this study is management and accounting master students who act as the surrogate of project manager. Used ANOVA as the data analysis technique, this study found a main effect of adverse selection and PsyCap in predict the escalation of commitment. However, there is no effect of the interaction between those two variables on escalation of commitment. This study contributes to the literature of escalation of commitment as well as psychological capital literature, considering that PsyCap is always associated with positive outcome but this study found that the high PsyCap might also predict undesirable outcome such as escalation of commitment. ABSTRAK Penelitian ini mengkaji determinan variabel struktural (adverse selection) dan variabel psikologis (psychological capital – PsyCap) pada kecenderungan manajer untuk meningkatkan komitmennya. Penelitian ini menggunakan rancangan eksperimen faktorial 2 x 2. Partisipan dalam penelitian ini adalah mahasiswa magister manajemen dan akuntansi yang bertindak sebagai penyulih manajer proyek. Menggunakan ANOVA sebagai teknik analisis data, penelitian ini menemukan efek utama dari adverse selection dan PsyCap dalam memprediksi eskalasi komitmen. Namun, tidak ada pengaruh interaksi antara kedua variabel tersebut terhadap eskalasi komitmen. Studi ini berkontribusi pada literatur eskalasi komitmen serta literatur modal psikologis, mengingat PsyCap selalu dikaitkan dengan hasil positif tetapi penelitian ini menemukan bahwa PsyCap yang tinggi mungkin juga dapat memprediksi hasil yang tidak diinginkan seperti eskalasi komitmen. Kunci: Kata adverse komitmen; selection; eskalasi psychological capital ; keagenan teori JIA (Jurnal Ilmiah Akuntansi) • Vol. 6, No. 1, Hal: 01-21 • Juni 2021
CUP_RAM_1800040 1..14

Most agree that models of binary time-series-cross-sectional data in political science often possess unobserved unit-level heterogeneity. Despite this, there is no clear consensus on how best to account for these potential unit effects, with many of the issues confronted seemingly misunderstood. For example, one oft-discussed concern with rare events data is the elimination of no-event units from the sample when estimating fixed effects models. Many argue that this is a reason to eschew fixed effects in favor of pooled or random effects models. We revisit this issue and clarify that the main concern with fixed effects models of rare events data is not inaccurate or inefficient coefficient estimation, but instead biased marginal effects. In short, only evaluating event-experiencing units gives an inaccurate estimate of the baseline risk, yielding inaccurate (often inflated) estimates of predictor effects. As a solution, we propose a penalized maximum likelihood fixed effects (PML-FE) estimator, which retains the complete sample by providing finite estimates of the fixed effects for each unit. We explore the small sample performance of PML-FE versus common alternatives via Monte Carlo simulations, evaluating the accuracy of both parameter and effects estimates. Finally, we illustrate our method with a model of civil war onset.
Paquetes as Improvised Media: Transnationalism and Cultural Consumption in Havana-Miami Context

Mediatic gambiarras are alternative and improvised forms of media consumption. They emerge in adverse or restrictive contexts. Signal the need for connection on the part of those who use them. In the island of Cuba, paquetes, sets of files containing pop culture content, music, series, US blockbusters, among others, not shown in Cuban media systems, are the most established cultural form of mediatic gambiarra on the socialist island. The article presents the result of a research project carried out between the years 2015 and 2017, based on an ethnographic-inspired methodology, with field interviews and participant observation in the city of Havana, with the purpose of debating different uses of the paquetes by consumers and fans of pop culture in the Cuban context. Contradictions about the practices of paqueteros — subjects who sell paquetes in online and offline network creations — are mapped from the emergence of traces of capitalism in Cuba that pass through the always conflicting relationship between residents in Havana and migrants in Miami in symbolic disputes on the island. The questions raised in the field signal for different matrixes around transnationalism in Latin America, geopolitical issues and point to understandings about the singularities and challenges of contemporary Cuba.
PREDICTORS OF MORTALITY IN ACUTELY INTOXICATED PATIENTS ADMITTED TO INTENSIVE CARE UNIT IN MENOUFIA UNIVERSITY HOSPITAL

Background: Acute poisoning is a major cause of suicide and public health problem all over the world. Evaluation of the morbidity and outcome of admitted patients can assess the efficacy of treatment, making it possible to take better decisions and to further improve quality of care. Objectives: The present study was aimed to study multiple factors associated with fatal poisoning of the patients admitted to ICU and to assess APACHE II Scoring System in prediction of poisoning outcome. Material and Methods: in this prospective study, from January 2019 to December 2019, critical patients with acute poisoning admitted to ICU, Menoufia University hospitals-Egypt were enrolled into this study. Data including demographic features, clinical and lab findings, Acute Physiology and Chronic Health Evaluation (APACHE II) at admission, mode and route of poisoning, the need for mechanical ventilation or vasoactive agents, duration of mechanical ventilation and ICU stay were documented, collected and analyzed by the relevant statistical methods. All patients were studied till death or discharge from the ICU. Results: During the study period, 152 out of 1745 patients admitted to the Menoufia poisoning control center (MPCC) were admitted to the ICU. Out of them, 54 died and 98 survived. The majority were in age group (20-40) years. Females were (62.5%) of the total cases. Intentional poisoning was predominant. Pesticides were the most commonly employed poison including aluminium phosphide and organophosphorus compounds. Aluminium phosphide was highly toxic. High APACHE II score, mechanical ventilation and inotropic support were associated with high mortality. Conclusion: Some findings were correlated with outcome in acute poisoning and can be used as guidance for risk assessment and treatment planning of the patients. The higher the APACHE II score, the more serious the condition becomes. Proper counseling to counteract the stressful situations to reduce fatalities. In addition, some policy should be applied to control the easy availability of poisons in the society.
Tectonic Stress Distribution in the Song Tranh 2 Hydropower Reservoir: Implication for Induced Earthquake

The Song Tranh 2 hydropower reservoir was built in Tra My area, Quang Nam province, composing magmatic and high-grade metamorphic rocks of the northern part of the Kon Tum massif. Since the reservoir was put into operation, induced earthquakes have occurred in the Song Tranh 2 hydropower reservoir and its vicinity. Tectonically, the northwest-southeast to east-west striking faults developed strongly. Detailed analysis of slickensides and attitude of faults occurring in the studied area have shown that the northwest-southeast striking faults are reactivated as dextral ones during the Pliocene-Quaternary up to the present day. Based on the geometric distribution of the fault network, kinematic characteristics, and the youngest tectonic stress regime, we computed the distribution of tectonic stress in the studied area. Computation results show two positive anomalies of stress directly related to the northwest-southeast faults numbered 2, 10, 11a, 11b and sub-latitude striking fault numbered 1. These faults run in line with the local river channels and are likely to reactivate and generate induced earthquakes.
Prospects for the Mechanism of Spiroplasma Swimming

Spiroplasma are helical bacteria that lack a peptidoglycan layer. They are widespread globally as parasites of arthropods and plants. Their infectious processes and survival are most likely supported by their unique swimming system, which is unrelated to well-known bacterial motility systems such as flagella and pili. Spiroplasma swims by switching the left- and right-handed helical cell body alternately from the cell front. The kinks generated by the helicity shift travel down along the cell axis and rotate the cell body posterior to the kink position like a screw, pushing the water backward and propelling the cell body forward. An internal structure called the “ribbon” has been focused to elucidate the mechanisms for the cell helicity formation and swimming. The ribbon is composed of Spiroplasma-specific fibril protein and a bacterial actin, MreB. Here, we propose a model for helicity-switching swimming focusing on the ribbon, in which MreBs generate a force like a bimetallic strip based on ATP energy and switch the handedness of helical fibril filaments. Cooperative changes of these filaments cause helicity to shift down the cell axis. Interestingly, unlike other motility systems, the fibril protein and Spiroplasma MreBs can be traced back to their ancestors. The fibril protein has evolved from methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase, which is essential for growth, and MreBs, which function as a scaffold for peptidoglycan synthesis in walled bacteria.
Drugs and chemicals use in frog farming in Dong Thap province

Received 22 Feb 2021 Revised 19 May 2021 Accepted 04 Jun 2021 The study was conducted to investigate the use of chemicals, drugs, and disease occurrence from frog farming in Dong Thap province. Data were collected through a questionnaire from a random sample of 30 frog farmers from January to April 2020. The results showed that the common diseases were pus in liver (66.7%), hemorrhage (66.7%), and digestive tract problems (flatulence, abdominal intestine, 40%). Farmers used antibiotics to treat diseases and chemicals to treat parasitic infection without knowledge on pathogens. Commonly used antibiotics were oxytetracycline (53.3%), doxycycline (63.3%), amoxicillin (53.3%), florfenicol (36.7%), and sulfamethoxazole/trimethoprim (46.7%). In addition, other antibiotics such as tetracycline, enrofloxacin, ciprofloxacin, rifampicin were used in some households. Farmers used iodine (26.7%), copper sulfate (20%), and BKC (16.7%) as disinfectants. There was no antibiotic residues test prior to sell to traders. It is necessary to support farmers in knowledge on diseases, drugs/chemicals use, and safety in the use of drugs and chemicals. Keywords
Comparative and Evolutionary Analyses on the Complete Plastomes of Five Kalanchoe Horticultural Plants

Many species of the genus Kalanchoe are important horticultural plants. They have evolved the Crassulacean acid metabolism (CAM) photosynthetic pathway to allow them to be better adapted to dry environments. Despite their importance, it is still debating whether Kalanchoe is monophyletic, and understanding the past diversification of this genus requires a tremendous amount of effort and work being devoted to the studies of morphological and molecular characters of this genus. However, molecular information, plastic sequence data, in particular, reported on Kalanchoe species is scarce, and this has posed a great challenge in trying to interpret the evolutionary history of this genus. In this study, plastomes of the five Kalanchoe species, including Kalanchoe daigremontiana, Kalanchoe delagoensis, Kalanchoe fedtschenkoi, Kalanchoe longiflora, and Kalanchoe pinnata, were sequenced and analyzed. The results indicate that the five plastomes are comparable in size, guanine-cytosine (GC) contents and the number of genes, which also demonstrate an insignificant difference in comparison with other species from the family Crassulaceae. About 224 simple sequence repeats (SSRs) and 144 long repeats were identified in the five plastomes, and most of these are distributed in the inverted repeat regions. In addition, highly divergent regions containing either single nucleotide polymorphism (SNP) or insertion or deletion (InDel) mutations are discovered, which could be potentially used for establishing phylogenetic relationships among members of the Kalanchoe genus in future studies. Furthermore, phylogenetic analyses suggest that Bryophyllum should be placed into one single genus as Kalanchoe. Further genomic analyses also reveal that several genes are undergone positive selection. Among them, 11 genes are involved in important cellular processes, such as cell survival, electron transfer, and may have played indispensable roles in the adaptive evolution of Kalanchoe to dry environments.
Temporal Distribution and Abundance of Mosquito Vectors in Dhaka City

Species of Anopheles, Aedes and Culex mosquitoes showed that Anopheles gambiae s.s had the highest number (43.5%) out of the three malaria vectors (viz. Anopheles gambiae, An. arabiensis and An. funestus). For Aedes and Culex species, Aedes aegypti (37.6%) and Culex fatigans (37.1%) had the highest prevalence out of their sibling species. Temperature and rainfall were highly correlated with the abundance of mosquito vectors. It was observed that the rainy season (March to October) recorded the highest number (Total 11 specie) of mosquito vectors collected with the peak (Aedes aegypty, 140) in the months of July (932) and August (976) while the lowest (333) collection was in the dry season (November to February) with lowest (333) in the month of February when there was little or no rains.