Miracle-dz/UniFAR-FTUs huggingface dataset:
Age, growth and maturity of the brown stingray (Dasyatis lata) around Oahu, Hawai'i

Baseline data on life-history characteristics of elasmobranchs are often lacking before fisheries exploitation, hindering successful management and conservation of affected species. Age, growth and maturity estimates were determined for the brown stingray, Dasyatis lata, an abundant benthic predator in Hawai’i’s coastal ecosystems, from an unfished population off Oahu, Hawai’i. Age estimates ranged from 0 to 28 years for females and from 0 to 25 years for males. Annual growth-band deposition was verified through marginal increment and centrum edge analysis, as well as recapture of two stingrays marked with oxytetracycline. Multiple growth models were fitted to disk width (DW) and weight-at-age data and evaluated for biological realism and statistical fit. Logistic growth functions best described growth of male and female stingrays, whereas no support was found for von Bertalanffy growth models. Females attained larger sizes (L∞) and exhibited lower growth coefficients (k) than did males. Median size and age at first maturity was estimated to be 104.9 cm DW (15 years) for females and 74.9 cm DW (8.3 years) for males. These life-history characteristics highlight the importance of obtaining baseline data before fisheries are established and indicate that any fishery for brown stingrays should be managed using conservative management strategies.
Isolation, genetic diversity and identification of a virulent pathogen of eriophyid mite, Aceria guerreronis (Acari: Eriophyidae) by DNA marker in Karnataka, India

Aceria guerreronis is a serious pest of coconut in India. Investigations were carried out to investigate fungal pathogens infecting the eriophyid mites for their utilisation as biocontrol agents in Karnataka, India. The fungal pathogens namely, H irsutella thompsonii, Beauveria bassiana, Fusarium semitectum and few opportunistic pathogens namely, Fusarium moniliforme, Cladosporium tennuissimum, Aspergillus niger, Penicillium sp. and Mucor sp . were collected from eriophyid mite populations in different parts of Karnataka area. Of the total collected nuts, 3.54% were infected by H. thompsonii , 2.46 and 0.29% by B. bassiana and F. semitectum , respectively. A lower number of nuts (0.03 to 0.79%) were infected by opportunistic pathogens. The incidence of pathogen infected coconuts in areas with lower temperature and higher humidity were ranged from 4.37 to 19.52% whereas with higher temperature and lower humidity it was 0 to 4.54%. Occurrence of B. bassiana and F. semitectum on A. guerreronis are new records. Among isolates of H. thompsonii collected from different places, the isolate Bangalore was more virulent followed by Mysore, Mandya, Kanakapura, Arsikere and Hiriyur isolates, as it recorded maximum infection (HTCMBAN- 88.63%). The B. bassiana isolates caused mortality ranging from 72.87 to 86.97%. The virulent isolate H. thompsonii (HTCMBAN) was tested at different concentrations, with increase in the concentration mortality rate. The genetic diversity of isolates of H. thompsonii by random amplification of polymorphic DNA (RAPD) revealed that grouping of the isolates was in accordance with geographic location. DNA fragments of 850 and 950 bp (OPA-20) were specific to the virulent isolate HTCMBAN. Key words : Fungal pathogens, eriophyid mites, genetic diversity, DNA marker.
IL-6 promotes PD-L1 expression in monocytes and macrophages by decreasing protein tyrosine phosphatase receptor type O expression in human hepatocellular carcinoma

Background We have previously discovered a relationship between the low expression of protein tyrosine phosphatase, receptor type O (PTPRO) in tumor-infiltrating T cells and immunosuppression. The aim of the present study was to investigate the relationship between decreased PTPRO and increased programmed death ligand 1 (PD-L1) in both the peripheral monocytes and tumor-infiltrating macrophages of human hepatocellular carcinoma (HCC). Methods The expression and correlation of all the indices were explored in monocytes and tumor-infiltrating macrophages within both human and mice HCC. The mechanic regulations were studied by using both in vitro and in vivo studies. Results We found a significant decrease in PTPRO in HCC peripheral monocytes that was associated with increased PD-L1 expression in peripheral monocytes and tumor-associated macrophages (TAMs) in HCC. Monocyte PD-L1 and PTPRO therefore could serve as valuable prognostic indicators for post-surgery patients with HCC and were associated with increased T-cell exhaustion (Tim3+T cells). A depletion of PTPRO promoted PD-L1 secretion in both monocytes and macrophages through the JAK2/STAT1 and JAK2/STAT3/c-MYC pathways. Increased IL-6 expression was associated with activation of JAK2/STAT3/c-MYC and with decreased PTPRO expression through the STAT3/c-MYC/miR-25–3 p axis. Monocytes and TAMs showed significantly increased miR-25–3 p expression, which could target the 3′ untranslated region of PTPRO. The miR-25–3 p expression positively correlated with serum IL-6 levels, but inversely correlated with PTPRO in HCC monocytes. IL-6/STAT3/c-MYC activation enhanced in vitro miR-25–3 p transcription and decreased PTPRO, while further promoting PD-L1 secretion. Adoptive cell transfer of c-MYC/miR-25–3 p–modified monocytes promoted tumor growth by downregulating PTPRO and causing a PD-L1–induced immunosuppression in an orthotopic tumor transplantation model. Conclusions Increased serum IL-6 downregulated PTPRO expression in HCC monocytes and macrophages by activating STAT3/c-MYC/miR-25–3 p and by further enhancing PD-L1 expression through JAK2/STAT1 and JAK2/STAT3/c-MYC signaling.
Physical surrogates for macrofaunal distributions and abundance in a tropical gulf

The characterisation of benthic habitats based on their abiotic (physical and chemical) attributes remains poorly defined in the marine environment, but is becoming increasingly central in the development of marine management plans in Australia and elsewhere in the world. The current study tested this link between physical and biological datasets for the southern Gulf of Carpentaria, Australia. The results presented were based on a range of physical factors, including the sediment composition (grain size and carbonate content), sediment mobility, water depth and organic carbon flux, and their relationship to the distribution and diversity of benthic macrofauna was tested. The results reveal the importance of process-based indices, such as sediment mobility, in addition to other environmental factors in defining the distribution of the benthic macrofauna. The distribution of the benthic macrofauna changes gradationally across the south-eastern Gulf, associated with changes in the per cent mud and gravel, the seabed exposure and the water depth. Patterns of diversity also reveal the importance of physical processes such as sediment mobility in defining benthic habitats. The species-environment relationships observed at the small scale of the current study are consistent with broader associations observed for other organisms within the Gulf.
MATING BEHAVIOR OF RHAGOLETIS POMONELLA (DIPTERA: TEPHRITIDAE) VI. SITE OF EARLY-SEASON ENCOUNTERS

Abstract Field observations of Rhagoletis pomonella (Walsh) adults on apple and hawthorn trees revealed that mating encounters occur on leaves in early-season and shift to fruit with the onset of oviposition. Most matings on leaves are initiated from a male frontal approach to the female and most of those on fruit from a rear approach while the female is engaged in some phase of oviposition behavior. This suggests that matings on fruit may be forced matings with unreceptive females. Résumé Des observations de terrain effectuées sur le pommier et l’aubépine montrent que la rencontre des sexes chez la mouche de la pomme, Rhagoletis pomonella (Walsh) a lieu sur le feuillage en début de saison, se déplaçant ensuite sur le fruit avec l’arrivée de la période de ponte. La majorité des accouplements sur le feuillage sont initiés par un mâle approchant une femelle de front, alors que sur le fruit, la majorité le sont suite à une approche de derrieère alors que la femelle est engagée dans une phase quelconque du comportement associé à la ponte. Ceci indiquerait que les accouplements ayant lieu sur le fruit sont possiblement des accouplements forcés de femelles non-réceptives.
Bioavailability of ascorbyl phosphate calcium in hybrid catfish, Clarias macrocephalus (Gunther) × Clarias gariepinus (Burchell) feed

A 10-week feeding experiment was performed in hybrid catfish (Clarias macrocephalus (Gunther) 3 C. gariepinus (Burchell, 1822) with initial average weight of 1.17 g, in glass aquaria of 200-L capacity. The experiment comprised seven treatments with three replicates each. Ascorbyl phosphate calcium (APCa) was included at 30, 75, 150, 300 and 1000 mg kg ‐1 feed and compared with one with ascorbyl polyphosphate (APP) at 36 mg kg ‐1 feed and one without vitamin C supplementation. Fish fed APCa-supplemented feed showed better growth, feed conversion ratio, survival rate and protein utilization compared with those without such supplementation. Ascorbate concentrations in liver and head kidneys were correlated closely with dietary APCa levels. Vertebral collagen and hydroxyproline contents were significantly lower in the fish group without ascorbate supplementation. Red blood cell and white blood cell counts showed no correlation with vitamin C supplementation. Serum protein level was lowest in the group fed feed without vitamin C supplementation and this was lower than those fed feed with APCa and APP supplementation. The fish fed APCa- and APPsupplemented feed showed no difference in serum protein levels. External scorbutic symptoms and histopathological changes of gill tissue were noted in fish fed feed without APCa supplementation. The study indicates that 12.6 mg kg ‐1 of ascorbic acid molar equivalent supplied as APCa (30 mg kg ‐1 ) provided maximum growth and no gross signs as well as histological changes resulting from vitamin C deficiency.
Multimodal approach of venous recanalization in patients with a critical limb ischemia due to phlegmasia cerulea dolens: A case series of 17 patients in a single center

Objectives Advanced phlegmasia cerulea dolens can be a hazardous complication of a deep vein thrombosis and rapid recanalization of the deep venous system is the most important factor. Method We describe the outcome of 17 patients with critical limb ischemia due to an advanced phlegmasia cerulea dolens. Venous thrombectomy was performed by a standardized operating procedure. Results Venous recanalization was successful in all patients. An additional fasciotomy was not necessary. There were five patients with an underlying malignancy and eight patients with a simultaneous pulmonary embolism. We had one amputation of a forefoot and one death within 30 days representing a 30-day mortality and an amputation rate of 6%. Conclusions Early recanalization and recovery of the venous outflow is mandatory for success. A multimodal therapeutic approach of high urgency surgical thrombectomy in combination with endovenous strategies could be a successful treatment option for advanced phlegmasia cerulea dolens.
Isavuconazole Treatment in a Mixed Patient Cohort with Invasive Fungal Infections: Outcome, Tolerability and Clinical Implications of Isavuconazole Plasma Concentrations

Isavuconazole (ISA) is a triazole antifungal agent recommended for treatment of invasive aspergillosis or mucormycosis. The objective of this study was to evaluate ISA levels in a real world setting in a mixed patient cohort including patients with non-malignant diseases and extracorporeal treatments, and to correlate findings with efficacy and safety outcomes. We investigated 33 ISA treatment courses in 32 adult patients with hematological and other underlying diseases and assessed the clinical response, side effects and ISA trough plasma concentrations. ISA treatment led to complete and partial response in 87% of patients and was well tolerated. The median ISA plasma concentration was 3.05 µg/mL (range 1.38–9.1, IQR 1.93–4.35) in patients without renal replacement therapy (RRT) or extracorporeal membrane oxygenation (ECMO) and significantly lower in patients with RRT including cases with additional ECMO or Cytosorb® adsorber therapy (0.88 µg/mL, range 0.57–2.44, IQR 0.71–1.21). After exclusion of values obtained from four patients with ECMO or Cytosorb® adsorber the median concentration was 0.91 µg/mL (range 0.75–2.44, IQR 0.90–1.36) in the RRT group. In addition to previous recommendations we propose to monitor ISA trough plasma concentrations in certain circumstances including RRT, other extracorporeal treatments and obesity.
Optimization of Short-Term Hot-Water Treatment of Apples for Fruit Salad Production by Non-Invasive Chlorophyll-Fluorescence Imaging

For fresh-cut salad production, hot-water treatment (HWT) needs optimization in terms of temperature and duration to guarantee a gentle and non-stressing processing to fully retain product quality besides an effective sanitation. One major initial target of heat treatment is photosynthesis, making it a suitable and sensitive marker for HWT effects. Chlorophyll fluorescence imaging (CFI) is a rapid and non-invasive tool to evaluate respective plant responses. Following practical applications in fruit salad production, apples of colored and of green-ripe cultivars (‘Braeburn’, ‘Fuji’, ‘Greenstar’, ‘Granny Smith’), obtained from a local fruit salad producer, were hot-water treated from 44 to 70 °C for 30 to 300 s. One day after HWT and after 7 days of storage at 4 °C, CFI and remission spectroscopy were applied to evaluating temperature effects on photosynthetic activity, on contents of fruit pigments (chlorophylls, anthocyanins), and on various relevant quality parameters of intact apples. In ‘Braeburn’ apples, short-term HWT at 55 °C for 30 to 120 s avoided any heat injuries and quality losses. The samples of the other three cultivars turned out to be less sensitive and may be short-term heat-treated at temperatures of up to 60 °C for the same time. CFI proved to be a rapid, sensitive, and effective tool for process optimization of apples, closely reflecting the cultivar- or batch-specificity of heat effects on produce photosynthesis.
Factors Associated With the Work Intention of Hospital Workers’ in South Korea During the Early Stages of the COVID-19 Outbreak

ABSTRACT Objective: This study aims to explore factors associated with the work intention of hospital workers in the early stages of the coronavirus disease (COVID-19) outbreak in South Korea. Methods: An online self-reported survey was conducted in a tertiary hospital. Respondents were asked to report their perceived threat and perceived risk of infection, evaluation of hospital response, demographics, and job-related factors. Descriptive statistics and multivariate regression analyses were performed. Results: A total of 441 employees participated in this study. Of respondents, 60% were willing to accept their work during an infectious disease outbreak and 12.5% were unwilling to accept the work. In addition, 8% of respondents reported that they had considered quitting their job, 54.4% reported that their job was dangerous, and 50.1% of respondents perceived the severity of COVID-19 as high. Perceived threat and effectiveness of hospital response were associated with hospital employees’ intention to work. Conclusions: Hospital workers are at the front line of the COVID-19 outbreak. This study highlighted hospital workers’ perceived effectiveness of organizational response to the outbreak, and perceived threats were found to be important factors for whether they continued to work or not in the fight against the outbreak.
Synergistic antitumor activity of a DLL4/VEGF bispecific therapeutic antibody in combination with irinotecan in gastric cancer

Notch signaling has been identified as a critical pathway in gastric cancer (GC) progression and metastasis, and inhibition of Delta-like ligand 4 (DLL4), a Notch ligand, is suggested as a potent therapeutic approach for GC. Expression of both DLL4 and vascular endothelial growth factor receptor 2 (VEGFR2) was similar in the malignant tissues of GC patients. We focused on vascular endothelial growth factor (VEGF), a known angiogenesis regulator and activator of DLL4. Here, we used ABL001, a DLL4/VEGF bispecific therapeutic antibody, and investigated its therapeutic effect in GC. Treatment with human DLL4 therapeutic antibody (anti-hDLL4) or ABL001 slightly reduced GC cell growth in monolayer culture; however, they significantly inhibited cell growth in 3D-culture, suggesting a reduction in the cancer stem cell population. Treatment with anti-hDLL4 or ABL001 also decreased GC cell migration and invasion. Moreover, the combined treatment of irinotecan with anti-hDLL4 or ABL001 showed synergistic antitumor activity. Both combination treatments further reduced cell growth in 3D-culture as well as cell invasion. Interestingly, the combination treatment of ABL001 with irinotecan synergistically reduced the GC burden in both xenograft and orthotopic mouse models. Collectively, DLL4 inhibition significantly decreased cell motility and stem-like phenotype and the combination treatment of DLL4/VEGF bispecific therapeutic antibody with irinotecan synergistically reduced the GC burden in mouse models. Our data suggest that ABL001 potentially represents a potent agent in GC therapy. Further biochemical and pre-clinical studies are needed for its application in the clinic.
Chironomid species richness of a permanent and a temporary Mediterranean stream: a long-term comparative study

Abstract Patterns of chironomid species richness were compared between 1 permanent and 1 temporary Mediterranean mountain stream in the same basin. From 1991 to 1995, pupal exuviae were collected at irregular intervals in the permanent stream, but the temporary stream was dry because of a long-lasting drought in the region. From March 1996 to March 2000, pupal exuviae were collected fortnightly in both streams. In contrast to other comparative studies that found more species in permanent than in temporary streams, our 2 streams harbored similar temporal γ and α diversity during the 1996 to 2000 study period. This result might be explained by the relatively long time the temporary stream was flowing. In addition, the temporary stream showed higher temporal β diversity than the permanent stream, a result that might be attributable to the greater variability in temperature and substrates in the temporary stream. Spates scarcely affected α diversity and favored colonization by new species in both streams, a result that supports the patch-dynamic concept of streams. A period of >1 y without spates also resulted in colonization by new species in the permanent stream, but led to a pool phase in the temporary stream that reduced α diversity considerably. Overall, both streams had similar chironomid species richness and species rarity and high faunal complementarity. These results support the view that temporary aquatic habitats deserve consideration in conservation plans designed to protect species and their habitats.
Neurocan genome‐wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans

Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders. Here, we investigated the potential effects of a genome‐wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers. We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts. In 117 participants, we performed functional magnetic resonance imaging using a novelty‐encoding task with visual scenes. Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation. To assess effects of rs1064395 on brain morphology, we performed voxel‐based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers. In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition. Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus‐dependent memory function, variation of prefrontal cortex structure and ECM composition. Considering the well‐documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
Dynamic characteristics of groups and individuals that amplify adherence to perceived drinking norms in college club sport teams: A longitudinal multilevel investigation.

Social norms positively predict college students' alcohol use, but it is critical to explore heterogeneity in these patterns to identify which students are most susceptible to normative influences. The current study explored the nature of drinking norms within college student peer sport clubs. We examined the association between self-reported alcohol use (i.e., number of drinks in a typical week) and perceived descriptive/injunctive norms as an indicator of norm adherence and then tested moderating effects of social constructs related to the group: Social identification with one's team, along with social network-derived indices of indegree centrality and network density. We sampled members of 35 intact college club sport teams at 3 timepoints across the school year (N = 1,054; 61% female). Multilevel modeling was employed to estimate moderating effects at within-person, between-person, and between-groups levels. Initial analyses revealed that perceived group norms predicted self-reported alcohol use, and that teams approached consensus on the groups' drinking norms over time. Several significant time-varying moderation patterns were uncovered. At timepoints when students identified more strongly with their team (relative to person-mean levels), they more readily adhered to perceived descriptive and injunctive team drinking norms. Students also adhered more closely to these perceived team drinking norms at timepoints when students were nominated as having relatively lower indegree centrality. Cross-level interactions revealed that neither network density nor team sex moderated these associations. Taken together, the current findings advance our understanding of group processes that may produce more salient social influences on students' alcohol use behaviors within proximal peer groups. (PsycInfo Database Record (c) 2020 APA, all rights reserved).