Miracle-dz/UniFAR-FTUs huggingface dataset:
PROTEINSTRUCTUREREPORT Non-3 D domain swapped crystal structure of truncated zebrafish alphaA crystallin

In previous work on truncated alpha crystallins (Laganowsky et al., Protein Sci 2010; 19:1031–1043), we determined crystal structures of the alpha crystallin core, a seven beta-stranded immunoglobulin-like domain, with its conserved C-terminal extension. These extensions swap into neighboring cores forming oligomeric assemblies. The extension is palindromic in sequence, binding in either of two directions. Here, we report the crystal structure of a truncated alphaA crystallin (AAC) from zebrafish (Danio rerio) revealing C-terminal extensions in a non threedimensional (3D) domain swapped, ‘‘closed’’ state. The extension is quasi-palindromic, bound within its own zebrafish core domain, lying in the opposite direction to that of bovine AAC, which is bound within an adjacent core domain (Laganowsky et al., Protein Sci 2010; 19:1031–1043). Our findings establish that the C-terminal extension of alpha crystallin proteins can be either 3D domain swapped or non-3D domain swapped. This duality provides another molecular mechanism for alpha crystallin proteins to maintain the polydispersity that is crucial for eye lens transparency.
Effect of prealbumin level on mortality in heatstroke patients

This study investigated whether serum prealbumin level is associated with mortality in heatstroke patients. A retrospective study of 102 heatstroke patients admitted to the ICU in the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University from June 2010 to November 2017 was performed they were divided into normal serum prealbumin group (n=79) and low prealbumin group (n=23) according to the difference in PA expression. The clinical data, laboratory inspection data, invasive positive pressure ventilation (IPPV), co-infection, shock and length of ICU stay during the ICU were compared between the two groups of patients. The study endpoints, deaths at admission, were recorded, and the survival curve plotted. Cox regression analysis was performed based on the clinical data of patients, and ROC curve plotted based on Cox multivariate independent prognostic indicators. There were significant differences in clinical variables PLT, ALT, AST, TBIL, ALB, TCH, LDH, TNI, BNP, creatinine, PT, APTT, FBG and D-dimer (P<0.05). The incidence of infection, shock and IPPV was significantly lower in normal serum prealbumin group of patients than those in low prealbumin group (P<0.05). There was a statistically significant difference in short-term survival rate between the groups of patients (χ2=29.101, P<0.001). Prognostic factors for heatstroke patients were IPPV, heart rate, WBC count, PLT count, ALB, PA, TBIL, LDH, CPK, Cr, PCT, PT, APTT, D-dimer, co-infection and shock at admission. Independent prognostic-related factors for heatstroke patients were IPPV, PA level, PLT level, ALB level, CPK level and PT level. When prealbumin <17.95 mg/dl was used as the death threshold for predicting at 28 days, the sensitivity was 77.8%, and the specificity was 85.7%. Significantly associated with the prognosis of heatstroke patients, prealbumin level can be used as an important predictive indicator of the disease progression and worse clinical outcomes.
Information and Communication Technologies Adoption in Small Firms

Information and Communication Technologies (ICTs) can provide a set of benefits to companies, such as efficiency gains in managerial activities, cost reduction and faster communication with consumers. The ICT literature has studies showing the positive impact that the IT adoption can generate on the company’s performance. ICT benefits are also extensive to small business, but their particular characteristics do not always support their adoption by these enterprises, especially when they are enterprises situated in developing countries. In this context, the aim of this paper was to identify how the ICT adoption and the ICT intention of use are in Brazilian small firms. We have used a case study and we have collected data from four enterprises. In order to collect the data, we used qualitative interviews, direct observation and documental analysis. The main results have shown that the owner’s characteristics, the understanding of the ICT benefits, the ICT complexity and the costs/time related to training people are factors associated with the ICT adoption and intention of use. Based on the literature review and on the empirical evidence, we have found that there is an opportunity cost related to postponing the decision of the ICT investments. We also discuss the role of geographical localization of the enterprises, which can contribute to the ICT literature.
Impact of Humic Acid on Yield and Quality of Sugar Beet ( Beta vulgaris L.) Grown on Calcareous Soil

A field experiment was performed to determine the effects of using humic acid with irrigation on quantitative and qualitative yield traits of sugar beet grown on calcareous soils in Esfaraen, located in the North-East of Iran. Treatments consisted of three levels of humic acid concentration (zero or control, 2.5 and 5 L ha -1 ) and the number of applications (once 45 days after planting; twice, 45 and 75 days after planting; three times, respectively 45, 75 and 105 days after planting). The effect of the two experimental factors on sugar beet yield traits such as the content of sucrose, refined sugar, molasses forming substances, root yield and refined sugar yield were evaluated. The results proved the existence of a significant interaction between humic acid concentration and the number of applications on all parameters under study. The application of humic acid caused a significant increase of sucrose, refined sugar, root yield and refined sugar yield and a reduction in molasses forming substances content, compared to the control. The increased amount of sucrose and refined sugar content in all applications in concentration of 2.5 L ha -1 humic acid was more than with 5 L ha -1 . Similarly, the content of molasses forming substances showed more reduction in 2.5 L ha -1 than in 5 L ha -1 treatment. However, the results revealed that the highest root yield and refined sugar yield, as the main qualitative and quantitative parameters of sugar beet yield, were achieved by three times application of 5 L ha -1 that had 24 and 37% increase compared to control.
Stratospheric water vapor : an important climate feedback 1

The role of stratospheric water vapor (SWV) changes, in response to in7 creasing CO2, as a feedback component of quantitative significance for climate sen8 sitivity has remained controversial. Here, we calculate the SWV climate feedback 9 under abrupt CO2 quadrupling in the CMIP5 ensemble of models. All models ro10 bustly show a moistening of the stratosphere, causing a global mean net stratosphere 11 adjusted radiative perturbation of 0.89±0.27 Wm−2 at the reference tropopause. The 12 stratospheric temperature adjustment is a crucial component of this radiative per13 turbation. The associated climate feedback is 0.17±0.05 Wm−2K−1, with a consid14 erable inter-model range of 0.12 to 0.28 Wm−2K−1. Taking into account the rise in 15 tropopause height under 4xCO2 slightly reduces the feedback to 0.15±0.04 Wm−2K−1, 16 with a range of 0.10 to 0.26 Wm−2K−1. The SWV radiative perturbation peaks in the 17 mid-latitudes and not the tropics: this is due primarily to increases in SWV in the 18 extratropical lowermost stratosphere, which cause the majority (over three quarters) 19 of the global mean feedback. Based on these results, we suggest an increased focus 20 on understanding drivers of water vapor trends in the extratropical lowermost strato21 sphere. We conclude that the SWV feedback is important, being on the same order of 22 magnitude as the global mean surface albedo and cloud feedbacks in the multi-model 23 mean. 24 A. Banerjee ( ,*) · G. Chiodo · L. M. Polvani Department of Applied Physics and Applied Mathematics, Columbia University, New York, NY, USA *now at National Oceanic and Atmospheric Administration/Earth Systems Research Laboratory/Chemical Sciences Division, Boulder, CO, USA and Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO, USA E-mail: antara.banerjee@noaa.gov M. Previdi Department of Earth and Environmental Sciences, Lamont Doherty Earth Observatory, Palisades, New York, NY, USA M. Ponater Deutsches Zentrum für Luftund Raumfahrt (DLR), Institut für Physik der Atmosphäre, Oberpfaffenhofen, 82230 Weßling, Germany A. J. Conley National Center for Atmospheric Research, Boulder, Colorado, USA 2 A. Banerjee et al.
Consolidation and prediction of long-term treatment effect of group and online mindfulness-based cognitive therapy for distressed cancer patients

Background: Group face-to-face and individual internet-based mindfulness-based cognitive therapy (MBCT and eMBCT) have been demonstrated to reduce psychological distress for distressed cancer patients in a randomized controlled trial (RCT). This study focused on the long-term effects of this RCT during the nine-month follow-up period, and on possible predictors, moderators and working mechanisms. Methods: Distressed cancer patients (n1⁄4 245) were randomized to MBCT or eMBCT. Data were collected at baseline, post-treatment, threeand nine-month follow-up. Data were analyzed with linear mixed effect models and (hierarchical) linear regressions. Results: Analyses revealed long-term reductions in psychological distress and rumination, and long-term increases in positive mental health and mental health-related quality of life (QoL) in both interventions over the course of the nine-month follow-up. Interestingly, patients reported less psychological distress in the follow-up period after eMBCT in comparison to MBCT. Less psychological distress, rumination and neuroticism, and more extraversion and agreeableness at baseline predicted less psychological distress at the nine-month follow-up after both interventions. Less mindful and conscientious patients at baseline benefited more from eMBCT than from MBCT. Regarding working mechanisms, changes in mindfulness skills, fear of cancer recurrence and rumination during both interventions predicted less psychological distress at follow-up. Conclusions: Our findings suggest most improvements in cancer patients’ increase over time after both interventions. Furthermore, patients seemed to benefit more from eMBCT than MBCT based on psychological distress levels, especially those patients with low levels of mindfulness skills and conscientiousness. ARTICLE HISTORY Received 18 April 2018 Accepted 14 May 2018
Value addition and storage study of khakhra fortification with garden cress seed (Lepidium sativum)

Khakhra is a popular vegetarian roasted ‘Gujarati’ Indian thin cracker bread or snack item made from whole-wheat flour and oil. In present investigation efforts were made to prepare khakhra by using different proportions and variety of flours i.e. S0, S1, S2, S3, and S4. The study was carried out to find out the development of value added khakhra by using different levels of flours whole-wheat flour, garden cress seed and buckwheat flour. The khakhra was prepared by wheat flour with substituting garden cress seed and buckwheat flour by increasing proportional 10%, 20%,30% and 40%. The nutritional value like fat, protein and iron content increases with increase the proportion of composite flour. In conclusion substitution of whole-wheat flour with garden cress seed flour and buckwheat flour will be the good nutritional food for iron deficiency people. The storage studies was carried out by three month and chemical composition checked by 30 days time of interval in that 30, 60,90 days.
P-wave and S-wave velocity structure of northern Cascadia margin gas hydrates

SUMMARY Single-channel seismic and wide-angle reflection data collected in September 2005 were analysed along a 2-D profile of 10 ocean bottom seismometers (OBSs) on the continental slope region off Vancouver Island, near ODP Site 889 and IODP Site U1327. The objectives were to determine the shallow P-wave and S-wave velocity structure associated with marine gas hydrates and to estimate the hydrate concentration and distribution in the sediment pore space. Combined traveltime inversion of single-channel and OBS data produced a P-wave velocity model down to the depth of the bottom-simulating reflector (BSR) at 230(±5) m below the seafloor (mbsf). Mean velocities, which increased from 1.50 km s−1 at the seafloor to 1.88 km s−1 at the BSR, are in good agreement with the sonic log data from Sites 889 and U1327. The increase in P-wave velocity of the hydrate-bearing sediments relative to a background no-hydrate velocity was utilized to estimate the hydrate concentration by using effective medium theory. An average concentration of 13 per cent in the interval from 120–230 mbsf was estimated from the P-wave velocity model. Lateral continuity of the model data confirms that these average hydrate concentrations are also found around the drillsites out to distances of a few kilometres. Forward modelling of S-waves was carried out using the data from the OBS horizontal components. Above the BSR, S-wave velocities are higher than a background velocity profile based on a rock physics model and on global averages for unconsolidated sediments. This increase in velocity suggests that the hydrate is distributed as part of the load-bearing matrix to increase the rigidity of the sediment.
Life after Default: Private vs. Official Sovereign Debt Restructurings

This paper studies the relationship between sovereign debt default and annual GDP growth taking into account the depth of a debt restructuring and distinguishing between private and official deals, as well as between debt flow and stock reduction. Analyzing 520 restructuring episodes, over the period 1975-2013, we find that private and official defaults may have different growth outcomes. Most importantly, controlling for the severity of the debt crisis, we are able to detect a more lasting and negative relationship between default and growth. While private defaults are generally associated with lower growth during the crisis and over the long run (mitigated by the amount involved), for official defaulters we do not observe a growth contraction throughout the years of the crisis and they are associated with higher growth over the long run (independently of the amount involved). When debt relief operations involve debt write offs, however, the negative relationship between private default and growth becomes blurred, while official defaulters strongly benefit in terms of growth from the face value reduction. Using the Synthetic Control Method, we present further evidence for the heterogeneity of the economic impact of debt restructurings, confirming that official and private defaults may have different effects on GDP growth and should then be treated differently.
Comparison of the meibomian gland dysfunction in patients with chronic ocular graft-versus-host disease and Sjögren's syndrome.

AIM To investigate the abnormalities in the meibomian gland in patients with dry eye disease (DED) associated with chronic ocular graft-versus-host disease (coGVHD) in comparison with Sjögren's syndrome (SS), a major form of aqueous deficient DED and meibomian gland dysfunction (MGD), a common cause of evaporative DED. METHODS A total 135 eyes of 135 subjects included in this study: patients with DED associated with coGVHD (n=30), patients with SS (n=35), patients with MGD (n=35), and normal controls (n=35). All participants completed the Ocular Surface Disease Index (OSDI) questionnaire, ocular surface examination [Schirmer test, tear film breakup time (TFBUT), and ocular surface staining], and meibomian gland assessment [meiboscore (gland dropout detected on meibography using infrared camera of the Keratograph 5M), meibum expressibility score (MES), meibum quality score (MQS), lid margin abnormality]. In addition, correlations of meibomian gland characteristics with ocular surface parameters as well as disease severity score were investigated in coGVHD group. RESULTS The coGVHD group showed significantly higher meiboscore, MES, and MQS than the other 3 groups (all P<0.05). In the coGVHD group, parameters of meibomian gland showed a significant correlation each other and those of ocular surface. The correlation between meibomian gland parameters and severity score of coGVHD was also established (meiboscore, r=0.62; MES, r=0.47; MQS, r=0.47; lid margin abnormality score, r=0.55; all P<0.05). CONCLUSION Patients with DED associated with coGVHD show poorer gland morphology and worse gland function than other types of DED. In addition, meibomian gland damage is not only associated with ocular surface damage but also disease severity of coGVHD.
Unravelling the Fuzzy Effect of Economic, Social and Environmental Sustainability on the Corporate Reputation of Public-Sector Organizations: A Case Study of Pakistan

The purpose of this study is to present and to validate a research model that includes economic sustainability, social sustainability, environmental sustainability and corporate reputation in the context of public-sector organizations in Pakistan. The methodological approach is survey-based, using partial least squares structural equation modelling (PLS-SEM) to assess the research model. The proposed hypotheses were tested based on a sample of 425 respondents from public-sector organizations in Pakistan. The findings of the study indicate that there is a positive relationship between economic sustainability, social sustainability, environmental sustainability and corporate reputation. Therefore, it is inferred from the results that different aspects of sustainability can create and enhance the reputation of public-sector organizations.
Development of Testing Standardization Regulation of the OLT XG-PON Equipments to Support Broadband Access in Indonesia

This study aims to provide a reference to the technical specifications of the Optical Line Termination (OLT) XG-PON equipment for improving standardization regulations (Perdirjen Postel No. 257 of 2008). The technical specifications tested in this study are the nominal rate capability, wavelength range, and jumbo frame of the OLT XG-PON equipment. The research acquired a reference to the nominal rate using FEC is 8.5 Gbps downstream direction, and 2.5 Gbps upstream direction, upstream wavelength range is 12601280 nm and downstream is 1575-1581 nm, and jumbo frame capability is 2000 Bytes.
Use of alternative organic compounds in the initial growth and quality of Anadenanthera colubrina (Vell. Brenan) seedlings

The use of renewable materials as a source of nutrients, besides being a solution of interest for waste disposal, may also be an effective way to reduce the high costs of inputs needed for the production of forestry seedlings. The purpose of this study was to evaluate the growth of Anadenanthera colubrina (Vell. Brenan) (angico branco) cultivated in organic substrates. A randomized block design was adopted and the treatments were distributed in a 3 × 5 factorial scheme, with three organic residues: decomposed burity (moriche palm) stem, carnaúba straw and organic compost mixed with the soil in four percentages: 20%, 40%, 60% and 80% of the composition of substrate and a control treatment with soil only, without incorporating the organic residue, with six repetitions. Ninety days after emergence, the seedlings were collected to determine the growth parameters: stem diameter (SD), height (H), of the following indexes: H/D, leaf number (LN), total chlorophyll (T), leaf area (LA), dry mass of the aerial part (DMAP), dry mass of roots (DMR), total dry mass (TDM) H/DMAP: DMAP/DMR, and Dickson Quality Index (DQI). The treatments that contained 60% and 80% of organic compost presented the best responses. It is concluded that the production of angico branco seedlings cultivated with substrate with the addition of 80:20 (organic compost: soil) presented the best results and was technically feasible according to the characteristics and indexes analyzed.
Why is sodium-intercalated graphite unstable?

Na-ion batteries offer an attractive low-cost alternative to Li-ion batteries. Although graphite is used as the negative electrode in conventional Li-ion batteries, attempts to use it in Na-ion batteries have been hampered by the inability of Na to form graphite intercalation compounds (GICs) under moderate conditions. It is generally considered that this is due to the size mismatch between Na and the graphite interlayer spacings, but here we show with detailed first-principles calculations of Li, Na, K, Rb, and Cs GICs that the major reason is the change in chemical bonding between alkali metal (AM) ion and C atoms. van der Waals correction terms are introduced to better reproduce the layered graphite structure, and calculated formation energies of GICs AMC6, where AM = Li, Na, K, Rb, and Cs, are found to become less negative (less stable) as ion size decreases from Cs to Na as a result of weakening ionic bonding, until the formation energy of NaC6 becomes positive. The much smaller Li ion represents an exception to this trend, as its bonds with C atoms contain a covalent component, resulting in a negative formation energy. These subtle differences in staging for the different alkali metals explain why graphite is a good intercalation material for Li and K but not for Na.
PINK 1 protein in normal human brain and Parkinson ’ s disease

Parkinson’s disease is a common incurable neurodegenerative disease whose molecular aetiology remains unclear. The identification of Mendelian genes causing rare familial forms of Parkinson’s disease has revealed novel proteins and pathways that are likely to be relevant in the pathogenesis of sporadic Parkinson’s disease. Recently, mutations in a novel gene, PINK1, encoding a 581 amino acid protein with both mitochondrial targeting and serine/threonine kinase domains, were identified as a cause of autosomal recessive parkinsonism. This provided important evidence for the role of the mitochondrial dysfunction and kinase pathways in neurodegeneration. In this study, we report the first characterization of the PINK1 protein in normal human and sporadic Parkinson’s brains, in addition to Parkinson’s cases with heterozygous PINK1 mutations. The possible role of the PINK1 protein was also assessed in a number of neurodegenerative diseases characterized by proteinaceous inclusions. For these studies, rabbit polyclonal antibodies were raised against two peptide sequences within the N-terminal hydrophilic loops of PINK1 protein. Using immunohistochemistry and western blotting we were able to demonstrate that PINK1 is a ubiquitous protein expressed throughout the human brain and it is found in all cell types showing a punctate cytoplasmic staining pattern consistent withmitochondrial localization. Fractionation studies of human and rat brain confirm that PINK1 is localized to the mitochondrial membranes. In addition, we show that PINK1 is detected in a proportion of Lewy bodies in cases of sporadic Parkinson’s disease and Parkinson’s disease associated with heterozygous mutations in the PINK1 gene, which are clinically and pathologically indistinguishable from the sporadic cases. PINK1 was absent in cortical Lewy bodies, in neurofibrillary tangles in Alzheimer’s disease, progressive supranuclear palsy and corticobasal degeneration, and in the glial and neuronal a-synuclein positive inclusions in multiple system atrophy. These studies provide for the first time in vivo morphological and biochemical evidence to support a mitochondrial localization of PINK1 and underpin the significance of mitochondrial dysfunction in the pathogenesis of nigral cell degeneration in Parkinson’s disease.