Miracle-dz/UniFAR-FTUs huggingface dataset:
Cholera toxin and O-specific polysaccharide immune responses after oral cholera vaccination with Dukoral in different age groups of Bangladeshi participants

ABSTRACT Vaccination is important to prevent cholera. There are limited data comparing anti-O-specific polysaccharide (OSP) and anti-cholera toxin-specific immune responses following oral whole-cell with cholera toxin B-subunit (WC-rBS) vaccine (Dukoral, Valneva) administration in different age groups. An understanding of the differences is relevant because young children are less well protected by oral cholera vaccines than older children and adults. We compared responses in 50 adults and 49 children (ages 2 to <18) who were administered two doses of WC-rBS at a standard 14-day interval. All age groups had significant IgA and IgG plasma-blast responses to the OSP and cholera toxin B-subunit (CtxB) antigens that peaked 7 days after vaccination. However, in adults and older children (ages 5 to <18), antibody responses directed at the OSP antigen were largely IgA and IgG, with a minimal IgM response, while younger children (ages 2 to <5) mounted significant increases in IgM with minimal increases in IgA and IgG antibody responses 30 days after vaccination. In adults, anti-OSP and CtxB memory B-cell responses were detected after completion of the vaccination series, while children only mounted CtxB-specific IgG memory B-cell responses and no OSP-memory B-cell responses. In summary, children and adults living in a cholera endemic area mounted different responses to the WC-rBS vaccine, which may be a result of more prior exposure to Vibrio cholerae in older participants. The absence of class-switched antibody responses and memory B-cell responses to OSP may explain why protection wanes more rapidly after vaccination in young children compared to older vaccinees. IMPORTANCE Vaccination is an important strategy to prevent cholera. Though immune responses targeting the OSP of V. cholerae are believed to mediate protection against cholera, there are limited data on anti-OSP responses after vaccination in different age groups, which is important as young children are not well protected by current oral cholera vaccines. In this study, we found that adults mounted memory B-cell responses to OSP, which were not seen in children. Adults and older children mounted class-switched (IgG and IgA) serum antibody responses to OSP, which were not seen in young children who had only IgM responses to OSP. The lack of class-switched antibody responses and memory B-cell responses to OSP in younger participants may be due to lack of prior exposure to V. cholerae and could explain why protection wanes more rapidly after vaccination in young children.
Improved Medication Adherence of an Elderly Diabetic Patient at a Dwelling Home Using a Pill Dispenser and Personal Health Records

Fookkun® is a pill dispenser in which single doses of several medications intended to be taken simultaneously are sealed in single film bags rolled onto a medication rotating drum. The system makes musical alert sounds when it is time for the patient to take the medications. If the patient misses a dose, a designated contact, such as the patient’s child, is alerted. We conducted an experiment monitoring the use of a pill dispenser (Fookkun®) by an older patient. The participant was a 71-year-old woman with diabetes living in a dwelling home. The experiment lasted approximately 6 months. Fookkun® and the prototype data transmitter were installed at the patient’s home. Fookkun®’s medication history data are displayed on the electronic medication record book (E-MRB) and the patient’s pharmacist checks the patient’s medication history on the E-MRB. The Fookkun® was effective in facilitating medication adherence. The pharmacist and the patient’s daughter did not need to check the E-MRB because Fookkun® alerted them when the patient missed her medication. We believe that if the medication history data linked between a pill dispenser and an E-MRB can be shared among medical staff, this will contribute to a medical digital transformation in Japan in the future.
Neural Dynamics based on EEG and diffusion MRI: Potential in studying stroke

After stroke, functional recovery may be promoted during the first six months through rehabilitation. Several events are thought to lead to regaining lost functions, among which the remapping of affected limbs to other regions of the cortex is a frequent occurrence. High spatial resolution brain imaging techniques, like magnetic resonance imaging (MRI), may be used to observe the affected areas and assess the severity of the lesions, but are unable to provide much insight on dynamic changes in brain function. An alternative non-invasive technique possessing an excellent temporal resolution to observe transient events is electroencephalography (EEG), a method limited by a low spatial resolution. This study aims to combine 62-channel EEG recordings with anatomical information derived from structural MRI and diffusion-weighted imaging (DWI) to improve the low resolution of EEG. During EEG acquisition, stroke patients (N = 3) and age-matched healthy controls (N = 2) received electrical impulses at both index fingers, stimulating both brain hemispheres sequentially through somatosensory feedback. After a static estimation of the current time series of candidate sources distributed over the brain cortex, a multivariate autoregressive (MAR) model was used to estimate the causal interactions between those found to be active due to the applied stimulus. The result was a visualization of the information transfer between active sources. A reasonably accurate estimation was achieved, even in presence of low signal to noise ratio (SNR) of EEG. Physiologically plausible source locations and connecting pathways were found, but an explanation of the observed phenomena and the interpretation of the differences between patients and controls is beyond the scope of this thesis, as a more extensive study is needed for this purpose. Consistent results found within subjects provide evidence of the potential value of this method in longitudinal studies, even when a comparison between subjects were not possible due to confounding factors (i.e. SNR differences). The obtained results support the candidacy of this method in the study of stroke, as it was found to be useful to track the information flow in the brain and might constitute a first step towards the development of a precise prognostic model of stroke.
Powders of Diamond Nanoparticles as a Promising Material for Reflectors of Very Cold and Cold Neutrons

More than 15 years ago, the study of nanodiamond (ND) powders as a material for designing reflectors of very cold neutrons (VCNs) and cold neutrons (CNs) began. Such reflectors can significantly increase the efficiency of using such neutrons and expand the scope of their application for solving applied and fundamental problems. This review considers the principle of operation of VCN and CN reflectors based on ND powders and their advantages. Information is presented on the performed experimental and theoretical studies of the effect of the size, structure, and composition of NDs on the efficiency of reflectors. Methods of chemical and mechanical treatments of powders in order to modify their chemical composition and structure are discussed. The aim is to avoid, or at least to decrease, the neutron inelastic scatterers and absorbers (mainly hydrogen atoms but also metallic impurities and nitrogen) as well as to enhance coherent elastic scattering (to destroy ND clusters and sp2 carbon shells on the ND surface that result from the preparation of NDs). Issues requiring further study are identified. They include deeper purification of NDs from impurities that can be activated in high radiation fluxes, the stability of NDs in high radiation fluxes, and upscaling methods for producing larger quantities of ND powders. Possible ways of solving these problems are proposed.
Effect of Hypotension and Dobutamine on Gastrointestinal Microcirculations of Healthy, Anesthetized Horses

Simple Summary When horses undergo abdominal surgery, there is a risk that tissues may not receive enough blood. One way to decide if this is happening is to measure blood pressure. The assumption is that if blood pressure is normal, then tissue blood flow is fine. A technique called dark-field microscopy has been used to look at the blood flow to tissues. We measured blood flow at three different places (the gums, the rectum, and the outside of the colon) of six healthy horses undergoing general anesthesia. Additionally, we measured how blood flow to these tissues changed when the patient’s blood pressure was lowered by giving an increased amount of gas anesthesia, which was subsequently raised with a drug called dobutamine. We found that blood flow to these tissues was present when the patients had normal blood pressure. When the blood pressure decreased, the blood flow to the tissues was unaltered. Finally, when blood pressure returned to normal, the blood flow to the tissues remained unaltered. Because blood flow to the tissue was unchanged despite clinically relevant changes in blood pressure, we concluded that using blood pressure as the only method to assess tissue blood flow may be inaccurate. Abstract Horses undergoing abdominal exploratory surgery are at risk of hypotension and hypoperfusion. Normal mean arterial pressure is used as a surrogate for adequate tissue perfusion. However, measures of systemic circulation may not be reflective of microcirculation. This study measured the mean arterial pressure, cardiac index, lactate, and four microcirculatory indices in six healthy, anesthetized adult horses undergoing elective laparotomies. The microcirculatory parameters were measured at three different sites along the gastrointestinal tract (oral mucosa, colonic serosa, and rectal mucosa) with dark-field microscopy. All macro- and microcirculatory parameters were obtained when the horses were normotensive, hypotensive, and when normotension returned following treatment with dobutamine. Hypotension was induced with increases in inhaled isoflurane. The horses successfully induced into hypotension did not demonstrate consistent, expected changes in systemic perfusion or microvascular perfusion parameters at any of the three measured gastrointestinal sites. Normotension was successfully restored with the use of dobutamine, while the systemic perfusion and microvascular perfusion parameters remained relatively unchanged. These findings suggest that the use of mean arterial pressure to make clinical decisions regarding perfusion may or may not be accurate.
Molecular identification, in vivo and in vitro activities of Calvatia gigantea (macro-fungus) as an antidiabetic agent

ABSTRACT Mushrooms are cherished as sources of food, nutrients and medicine. Inadequate data on the identity and medicinal properties of many wild Nigerian mushrooms has limited their utilization. This work was carried out to identify and authenticate a puffball mushroom using molecular tools and investigate its antidiabetic properties. Taxonomic guides were employed in morphological identifying the mushroom as Lycoperdon umbrinum, methanol extract of fruiting bodies was evaluated for antidiabetic activity using in vitro α-amylase assay and in vivo activity in the alloxan-induced diabetic rat model. The macro fungus was identified using Internal Transcribed Spacers (ITS) sequence analysis after which sequences generated were compared using the basic local alignment search tool (BLAST) at NCBI GenBank. In the acute in vivo test, the 400 mg/kg dose showed the best activity with percentage reduction in blood glucose 29.3%, compared with 5 mg/kg glibenclamide at 15%. The in vitro assay established that the extract possessed potent activity with IC50 of 0.46 µg/mL compared to its DCM, butanol fractions and acarbose (IC50 5.3 µg/mL, 5.6 µg/mL, 45 µg/mL) respectively. BLAST analysis revealed the mushroom (accession number, KRO78278.1) to show 98% identity to Calvatia gigantea. The study established the identity of this mushroom and confirmed its antidiabetic activity.
A novel oyster shell biocomposite for the efficient adsorptive removal of cadmium and lead from aqueous solution: Synthesis, process optimization, modelling and mechanism studies

This study highlights the effectiveness of oyster shell biocomposite for the biosorption of Cd(II) and Pb(II) ions from an aqueous solution. The aim of this work was to modify a novel biocomposite derived from oyster shell for the adsorption of Cd(II) and Pb(II) ions from aqueous solution. The studied revealed the specific surface BET surface area was 9.1476 m2/g. The elemental dispersive x-ray analysis (EDS) indicated that C, O, Ag, Ca were the predominant elements on the surface of the biocomposite after which metals ions of Cd and Pb were noticed after adsorption. The Fourier transform Irradiation (FT-IR) revealed the presence of carboxyl and hydroxyl groups on the surface. The effect of process variables on the adsorption capacity of the modified biocomposite was examined using the central composite design (CCD) of the response surface methodology (RSM). The process variables which include pH, adsorbent dose, the initial concentration and temperature were the most effective parameters influencing the uptake capacity. The optimal process conditions of these parameters were found to be pH, 5.57, adsorbent dose, 2.53 g/L, initial concentration, 46.76 mg/L and temperature 28.48°C for the biosorption of Cd(II) and Pb(II) ions from aqueous solution at a desirability coefficient of 1. The analysis of variance (ANOVA) revealed a high coefficient of determination (R2 > 0.91) and low probability coefficients for the responses (P < 0.05) which indicated the validity and aptness of the model for the biosorption of the metal ions. Experimental isotherm data fitted better to the Langmuir model and the kinetic data fitted better to the pseudo-second-order model. Maximun Cd(II) and Pb(II) adsorption capacities of the oyster shell biocomposite were 97.54 and 78.99 mg/g respectively and was obtained at pH 5.56 and 28.48°C. This investigation has provided the possibility of the utilization of alternative biocomposite as a sustainable approach for the biosorption of heavy metal ions from the wastewater stream.
Can the Brain’s Thermostatic Mechanism Generate Sleep-Wake and NREM-REM Sleep Cycles? A Nested Doll Model of Sleep-Regulating Processes

Evidence is gradually accumulating in support of the hypothesis that a process of thermostatic brain cooling and warming underlies sleep cycles, i.e., the alternations between non-rapid-eye-movement and rapid-eye-movement sleep throughout the sleep phase of the sleep-wake cycle. A mathematical thermostat model predicts an exponential shape of fluctuations in temperature above and below the desired temperature setpoint. If the thermostatic process underlies sleep cycles, can this model explain the mechanisms governing the sleep cyclicities in humans? The proposed nested doll model incorporates Process s generating sleep cycles into Process S generating sleep-wake cycles of the two-process model of sleep-wake regulation. Process s produces ultradian fluctuations around the setpoint, while Process S turns this setpoint up and down in accord with the durations of the preceding wake phase and the following sleep phase of the sleep-wake cycle, respectively. Predictions of the model were obtained in an in silico study and confirmed by simulations of oscillations of spectral electroencephalographic indexes of sleep regulation obtained from night sleep and multiple napping attempts. Only simple—inverse exponential and exponential—functions from the thermostatic model were used for predictions and simulations of rather complex and varying shapes of sleep cycles during an all-night sleep episode. To further test the proposed model, experiments on mammal species with monophasic sleep are required. If supported, this model can provide a valuable framework for understanding the involvement of sleep-wake regulatory processes in the mechanism of thermostatic brain cooling/warming.
Angelica keiskei water extract Mitigates Age-Associated Physiological Decline in Mice

ABSTRACT Objective Angelica keiskei is a medicinal and edible plant that has been reported to possess potent antioxidant properties in several in vitro models, but its effectiveness on naturally aging organisms is still lacking. This study explores the antioxidant and health-promoting effects of Angelica keiskei in naturally aging mice. Methods We treated 48-week-old mice with Angelica keiskei water extract (AKWE) 30 days, and measured indicators related to aging and antioxidants. In addition, we conducted network pharmacology analysis, component-target molecular docking, real-time PCR, and MTS assays to investigate relevant factors. Results The results indicated that administration of AKWE to mice led to decrease blood glucose levels, improve muscle fiber structure, muscle strength, gait stability, and increase levels of glutathione and superoxide dismutase in serum. Additionally, it decreased pigmentation of the heart tissues. Angelica keiskei combats oxidative stress by regulating multiple redox signaling pathways, and its ingredients Coumarin and Flavonoids have the potential to bind to SIRT3 and SIRT5. Conclusions Our findings indicated the potential of Angelica keiskei as a safe and effective dietary supplement to combat aging and revealed the broad prospects of medicinal and edible plants for addressing aging and age-related chronic diseases.
Alkali pretreatment of cotton stalk for bioethanol

Cotton stalk biomass was treated with NaOH and NaOH-steam pretreatements to get maximum cellulose content. Three factors with three levels such as biomass concentration (5, 10 and 15%), NaOH concentration (1, 3 and 5%) and residence time (4, 6 and 8 h) was performed through Box-Bhenken Design of response surface methodology. The treatment was performed with and without heating at 121oC for 15min and 15psi in an autoclave. Among these two types of treatment, maximum yield of cellulose content 87.80% was observed with 5% w/v NaOH concentration, 10g substrate loading and 4h residence time. The substrate having high cellulose content under optimized pretreatment conditions were analysed through FTIR revealing efficiency of pretreatment. The proposed model for this study was found significant in terms of lower p<0.05 values and findings of this study could be utilized for further processes like saccharification and fermentation to bioethanol. Bangladesh J. Sci. Ind. Res.54(1), 73-82, 2019
An Energy Efficiency Study of Web-Based Communication in Android Phones

Currently, mobile devices are the most popular pervasive computing devices, and they are becoming the primary way for accessing Internet. Battery is a critical resource in such personal computing gadgets, network communications being one of the primary energy consuming activities in any mobile app. Indeed, as web-based communication is the most used explicitly or implicitly by mobile devices, HTTP-based traffic is the most power demanding one. So, mobile web developers should be aware of how much energy demands the different web-based communication alternatives. The goal of this paper is to measure and compare the energy consumption of three asynchronous HTTP-based methods in mobile devices in different browsers. Our experiments focus on three HTTP-based asynchronous communication models that allow a web server to push data to a client browser through a HTTP/1.1 interaction: Polling, Long Polling, and WebSockets. The resulted measurements are then analysed to get more accurate understanding of the impact of the selected method, and the mobile browser, in the energy consumption of the asynchronous HTTP-based communication. The utility of these experiments is to show developers what are the factors and settings that mostly influence the energy consumption when different web-based asynchronous communication methods are used, helping them to choose the most beneficial solution if possible. With this information, mobile web developers should be able to reduce the power consumption of the front-end of web applications for mobile devices, just selecting and configuring the best asynchronous method or mobile browser, improving the performance of HTTP-based communication in terms of energy demand.
Two simultaneous anatomical variations of the cervical spine - a case report discussing the concept of tandem anomalies.

forming a bony opening through which the vertebral artery (VA) enters the vertebral canal. Block vertebra is a synostosis of at least two vertebral bodies that did not separate during the embryological development. It is worth distinguishing it from the Klippel-Feil syndrome, as the latter oftentimes involves other abnormalities (namely skeletal) and is typically diagnosed in childhood. Both variants could potentially lead to an impairment of the blood flow through the VA. Case report: The following case report presents a finding of two anomalies of the cervical spine, found in a 38 y.o. female patient suffering from dizziness. A synostosis of the C4 and C5 vertebral bodies, arches and zygapophysial (facet) joint, was noted by the examining radiologist, with marked narrowing of the intervertebral foramen. Furthermore, second anatomical variation in the form of the complete bilateral arcuate foramen was identified superior to the groove for the VA on the upper surface of the posterior arch of the atlas. Conclusions: To the best knowledge of the authors, this case report is the first to present a co-existing block vertebra and bilateral complete arcuate foramen. Common presence of at least two anatomical variations that could have a synergistic clinical effect could possibly be termed 'tandem anomaly. ' Notwithstanding, identification of a single anomaly explaining a patient's symptoms does not absolve the medical professionals from searching for any other potential variations that could also be present and could further influence the clinical picture.
Meyrowitzite, Ca(UO2)(CO3)2⋅5H2O, a new mineral with a novel uranyl-carbonate sheet

Abstract Meyrowitzite, Ca(UO2)(CO3)2⋅5H2O, is a new mineral species from the Markey mine, Red Canyon, San Juan County, Utah, U.S.A. It is a secondary phase found on calcite-veined asphaltum in association with gypsum, markeyite, and rozenite. Meyrowitzite occurs as blades up to about 0.2 mm in length, elongate on [010], flattened on {100}, and exhibiting the forms {100}, {001}, {101}, {110}, and {011}. The mineral is yellow and transparent with vitreous luster and very pale yellow streak. Fluorescence under a 405 nm laser is from weak greenish yellow to moderate greenish blue. The Mohs hardness is ca. 2, tenacity is brittle, fracture is irregular, and there is one perfect cleavage, {101}. The measured density is 2.70(2) g/cm3. The mineral is optically biaxial (+) with α = 1.520(2), β = 1.528(2), and γ = 1.561(2) (white light). The 2V(meas) = 53.0(6)°; weak dispersion, r > ν; optical orientation: Z = b, Y ^ a = 19° in obtuse β; pleochroism pale yellow, X ≈ Y < Z. Electron microprobe analyses provided the empirical formula Ca0.94(U1.00O2)(CO3)2⋅5(H2.02O) on the basis of U = 1 and O = 13 apfu, as indicated by the crystal structure determination. Meyrowitzite is monoclinic, P21/n, a = 12.376(3), b = 16.0867(14), c = 20.1340(17) Å, β = 107.679(13)°, V = 3819.3(12) Å3, and Z = 12. The structure (R1 = 0.055 for 3559 Io > 2σI) contains both UO7 pentagonal bipyramids and UO8 hexagonal bipyramids, the latter participating in uranyl tricarbonate clusters (UTC). The two kinds of bipyramids and the carbonate groups link to form a novel corrugated heteropolyhedral sheet. This is the first structural characterization of a uranyl-carbonate mineral with a U:C ratio of 1:2. Meyrowitzite is apparently dimorphous with zellerite.