Miracle-dz/UniFAR-FTUs huggingface dataset:
Adipose tissue derived stromal stem cell therapy in murine ConA‐derived hepatitis is dependent on myeloid‐lineage and CD4+ T‐cell suppression

Mesenchymal stromal stem cells (MSCs) are an attractive therapeutic model for regenerative medicine due to their pluripotency. MSCs are used as a treatment for several inflammatory diseases, including hepatitis. However, the detailed immunopathological impact of MSC treatment on liver disease, particularly for adipose tissue derived stromal stem cells (ADSCs), has not been described. Here, we investigated the immuno‐modulatory effect of ADSCs on hepatitis using an acute ConA C57BL/6 murine hepatitis model. i.v. administration of ADSCs simultaneously or 3 h post injection prevented and treated ConA‐induced hepatitis. Immunohistochemical analysis revealed higher numbers of CD11b+, Gr‐1+, and F4/80+ cells in the liver of ConA‐induced hepatitis mice was ameliorated after the administration of ADSCs. Hepatic expression of genes affected by ADSC administration indicated tissue regeneration‐related biological processes, affecting myeloid‐lineage immune‐mediating Gr‐1+ and CD11b+ cells. Pathway analysis of the genes expressed in ADSC‐treated hepatic inflammatory cells revealed the possible involvement of T cells and macrophages. TNF‐α and IFN‐γ expression was downregulated in hepatic CD4+ T cells isolated from hepatitis livers co‐cultured with ADSCs. Thus, the immunosuppressive effect of ADSCs in a C57BL/6 murine ConA hepatitis model was dependent primarily on the suppression of myeloid‐lineage cells and, in part, of CD4+ T cells.
Cross-Taxon Congruence of Taxonomic and Functional Beta-Diversity Facets Across Spatial and Temporal Scales

An intensively debated issue in ecology is whether the variations in the biodiversity patterns of different biological groups are congruent in space and time. In addition, ecologists have recognized the necessity of accounting for both taxonomic and functional facets when analysing spatial and temporal congruence patterns. This study aimed to determine how the cross-taxon congruence of taxonomic and functional beta diversity varies across space and time, using data from four floodplains at a continental scale. Our general hypothesis was that the congruence between aquatic biological groups, either taxonomic or functional, would decrease with the “between-group” functional distance. Also, we examined how congruence patterns varied across spatial and temporal scales by focusing on how the cross-taxon relationships differ among Brazilian floodplains and between dry/wet periods. Our study comprised information on eight biological groups from the four largest Brazilian river-floodplain systems, and cross-taxon congruence was assessed using Procrustes analysis. Our results show how detailed analyses can reveal different patterns of cross-taxon congruence, and partially support the hypothesis that the strength of cross-taxon congruence is negatively related to between-group functional distance.
The Adaptive Test of Emotion Knowledge for 3-to 9-Year-Olds: Psychometric Properties and Validity

Children with an advanced knowledge of emotions are generally more socially competent, less likely to suffer from psychopathology, and more likely to succeed in school, both socially and academically. The assessment of children's emotion knowledge has thus gained importance in recent decades - both in psychiatric practice and in developmental and educational psychology. However, there is still a lack of appropriate instruments for assessing children's emotion knowledge in a performance test reliably, and for a broad age range. The Adaptive Test of Emotion Knowledge (ATEM 3–9) is a newly developed measure which encompasses seven components of emotion knowledge in 3–9-year-olds. The ATEM 3–9 is an adaptive test which uses skip and dropout rules to adjust for children's varying levels of knowledge. In addition to German, the ATEM has been translated into English and Hebrew. The German norming sample of the ATEM 3–9 comprises N = 882 (54% female, 21% bilingual) children between the ages of 3 and 9 years, who were divided into seven age groups. Test items, which are ordered according to the item response theory, showed a good fit to a seven-dimensional model reflecting the seven components. The internal consistencies of the dimensions are acceptable to good. Construct validity was examined by means of correlations with other measures of emotion knowledge, as well as measures on language skills and executive functions in a subsample. This resulted in medium size correlations in the expected directions. In addition, children with externalizing and internalizing disorders who were recruited in psychiatric in- and outpatient clinics showed deficits in various components of emotion knowledge when compared to their agemates in the norming sample. Overall, the ATEM 3–9 is well suited to measure individual components of emotion knowledge in children and to obtain a differentiated picture of the various aspects of emotion knowledge. The ATEM 3–9 thus supports the investigation of the development of social-emotional competencies in normative development (e.g., school readiness) and in social-emotional-learning interventions. Furthermore, it is suitable as an instrument for the differentiated assessment of (progress of) children's emotion knowledge in clinical child psychology and psychiatry.
Prospects and Potential of Bradyrhizobium diazoefficiens Based Bio-Inoculants on Soybean Production in Different Agro-Ecologies of Mozambique

Soybean production in sub-Saharan Africa is increasing as farmers open more land areas for cultivation and replace other crops, such as tobacco, in favor of this legume crop. Despite the production is increased in Mozambique, demand for animal feed and oil is not satisfied. As such, farmers explore ways to improve yield per unit area of soybean by using bio-inoculants from various sources and agroecological adaptability. These bio-inoculants are seldom available during planting time, and retail at almost similar prices although yield varied based on the product source, handling, and the rhizobia strain carrier. Mozambique does not produce bio-inoculants, so it obtains the product from neighboring countries or as far as the South American continent. In this study, we evaluated the performance, ecological adaptability, and soybean productivity of seven Bradyrhizobium diazoefficiens strain-based bio-inoculants from several countries with different carrier materials: Biofix, Masterfix, Nitrofix, NitroZam, N-Fixer, Soygro Peat, and Soygro Liquid against a control (non-inoculated) on two soybean varieties Storm and TGx 1904-6F. The trial was conducted in the 2016 and 2017 growing seasons in three agroecologies of Mozambique at Angonia, Nampula, and Ruace. Data on nodulation, plant growth, biomass nitrogen content at beginning of podding (R3) stage, yield, and yield components of soybean were evaluated. Analysis of variance and contrast comparisons were performed on the Statistical Analysis System® 9.4. Nodule weight per plant variedly increased from 7.7 to 167.6 mg with inoculation of both varieties across environments. Plant tissue nitrogen content at the R3 stage was higher in inoculated non-promiscuous variety at 3.9% than the promiscuous counterpart with 3.7%. Storm, a non-promiscuous short-maturity variety of soybean, responded to inoculation and accumulated more N than the medium-to-late maturity promiscuous TGx 1904-6F. Higher N tissue content is an indicator of better nutritive value, as well as high-quality recyclable biomass of inoculated soybean. Both Storm and TGx 1904-6F responded to all inoculants variedly with NitroZam yield of 2,750 kg ha−1 being highest, while Soygro Liquid was lowest with 2,051 kg ha−1 but more than the check with 1,690 kg ha−1 across sites. There were varietal differences in 100-seed weight after inoculation where Storm (15.4 g) had heavier seeds than TGx 1904-6F (13.1 g). The results show that inoculation improved plant growth and development, increased nodulation, and gave higher yields for better economic returns among farmers. Inoculation has the potential of increasing soybean yield, nutritive value, and biomass quality within Mozambique.
Runoff Variation and Influencing Factors in the Kuye River Basin of the Middle Yellow River

In recent decades, due to climate change and human activities, the hydrological processes of many rivers in the world have undergone significant changes. As an important part of the water cycle, it is of great practical significance to identify the causes of runoff change for water resource management and planning. Taking the Kuye River and its tributaries, the Wulanmulun River and Beiniuchuan River as examples, the trend change in runoff was investigated by the Mann-Kendall trend test and mutation analysis, and the contribution of influencing factors of runoff change was quantitatively evaluated by the Budyko framework. The results showed that the annual runoff depth of the Kuye River basin and its tributaries, the Wulanmulun River basin and Beiniuchuan River basin, showed a significant decreasing trend from 1960 to 2014 (p < 0.01), and the decreasing rates were 1.03 mm/a, 1.24 mm/a and 1.50 mm/a, respectively. The abrupt change point of runoff depth in the Kuye River basin and its upstream Beiniuchuan River basin occurred in 1996, while that in the Wulanmulun River basin, another tributary, occurred in 1992. In the Kuye River basin, Beiniuchuan River basin and Wulanmulun River basin, the contributions of underlying surface change to runoff change were 89.03, 89.54, and 95.42%, respectively, followed by the contribution of rainfall, and the contribution of potential evapotranspiration to runoff change was the lowest. The change in the underlying surface (the Grain for Green Project and coal mining) is the main factor causing the decrease in runoff in the Kuye River basin.
Effects of Variability in Glycemic Indices on Longevity in Chinese Centenarians

Background Large fluctuations in blood glucose levels greatly impact the health and life span of elderly individuals. This study describes the characteristics of variability in glycemic indices in centenarians with the aim of emphasizing the importance of glycemic variability in elderly people. Methods We recruited individuals from Rugao City, Jiangsu Province, China from April 2020 to May 2021. The study cohort included 60 centenarians and 60 first-generation offspring, as well as 20 randomly selected non-cohabitant control individuals aged 60–80 years. A FreeStyle Libre H (hospital version) continuous glucose monitoring (CGM) device (Abbott Ireland UK) was used to measure glycemic variability. The indices measured included the time in target glucose range (TIR), time below target glucose range (TBR), time above target glucose range (TAR), mean amplitude of glycemic excursions (MAGE), mean of daily differences (MODD), coefficient of variation (CV), standard deviation of blood glucose (SDBG), continuous overlapping net glycemic action (CONGA), glucose management indicator (GMI) and estimated glycated hemoglobin (eHbA1c). Logistic regression was used to analyze the association between glycemic variability and longevity. Results Mean blood glucose (MBG), eHbA1c, GMI, mean fasting plasma glucose (M-FPG) and CONGA were lower in the centenarian group (p all < 0.05). PPGE-2 was higher in the control group than that measured in the centenarian and first-generation offspring groups (p < 0.05). There were no differences between the groups in MAGE, MODD, MAG, or TIR (p > 0.05). The risk of not achieving longevity increased with each one unit increase in MBG by 126% [2.26 (1.05–4.91)], eHbA1c by 67% [1.67 (1.03–2.72)], GMI by 568% [6.68 (1.11–40.30)], M-FPG by 365% [4.65 (1.57–13.75)], M-PPG1h by 98% [1.98 (1.18–3.31)], CONGA1 by 102% [2.02 (1.01–4.06)], Li by 200% [3.00 (1.04–8.61)], and PPGE-2 by 150% [2.50 (1.39–4.50)]. However, the risk of achieving longevity decreased with each unit increase of LBGI by 53% [0.47 (0.28–0.80)], ADRR by 60% [0.40 (0.18–0.86)], and TBR by 11% [0.89 (0.80–0.98)]. Conclusion Fluctuation in blood glucose levels in centenarians is relatively small. Maintaining an average blood glucose level and keeping blood glucose fluctuations in the normal range is conducive to longevity.
Ferroptosis in Intrahepatic Cholangiocarcinoma: IDH1105GGT Single Nucleotide Polymorphism Is Associated With Its Activation and Better Prognosis

Objectives Intrahepatic cholangiocarcinoma (ICC) has a dismal prognosis and often demonstrates an anti-apoptotic landscape, which is a key step to chemotherapy resistance. Isocitrate dehydrogenase 1 or 2 (IDH1-2)-mutated ICCs have been described and associated with better prognosis. Ferroptosis is a regulated iron-mediated cell death induced by glutathione peroxidase 4 (GPX4) inhibition, and may be triggered pharmacologically. GPX4 is overexpressed in aggressive cancers, while its expression is inhibited by IDH1R132C mutation in cell lines. We investigated tissue expression of ferroptosis activation markers in ICC and its correlation with clinical-pathological features and IDH1-2 status. Materials and Methods We enrolled 112 patients who underwent hepatic resection or diagnostic liver biopsy for ICC. Immunostaining for transferrin-receptor 1 and GPX4, and Pearls’ stain for iron deposits were performed to evaluate ferroptosis activation. Immunostaining for STAT3 was performed to study pro-inflammatory and anti-apoptotic landscape. Main IDH1-2 mutations were investigated in 90 cases by real-time polymerase chain reaction. Results GPX4 overexpression was seen in 79.5% of cases and related to poor histological prognostic factors (grading and perineural and vascular invasion; p < 0.005 for all) and worse prognosis (OS p = 0.03; DFS p = 0.01). STAT3 was expressed in 95.5% of cases, confirming the inflammation-related anti-apoptotic milieu in ICC, and directly related to GPX4 expression (p < 0.0001). A high STAT3 expression correlated to a worse prognosis (OS p = 0.02; DFS p = 0.001). Nearly 12% of cases showed IDH1105GGT single nucleotide polymorphism, which was never described in ICC up to now, and was related to lower tumor grade (p < 0.0001), longer overall survival (p = 0.04), and lower GPX4 levels (p = 0.001). Conclusion Our study demonstrates for the first time that in most inflammatory ICCs ferroptosis is not active, and its triggering is related to IDH1-2 status. This supports the possible therapeutic role of ferroptosis-inducer drugs in ICC patients, especially in drug-resistant cases.
Identifying Differential Expression Genes and Prognostic Signature Based on Subventricular Zone Involved Glioblastoma

Background: Studies have suggested that glioblastoma (GBM) cells originate from the subventricular zone (SVZ) and that GBM contact with the SVZ correlated with worse prognosis and higher recurrence. However, research on differentially expressed genes (DEGs) between GBM and the SVZ is lacking. Methods: We performed deep RNA sequencing on seven SVZ-involved GBMs and paired tumor-free SVZ tissues. DEGs and enrichment were assessed. We obtained GBM patient expression profiles and clinical data from the Chinese Glioma Genome Atlas (CGGA) and The Cancer Genome Atlas (TCGA) databases. The least absolute shrinkage and selection operator Cox regression model was utilized to construct a multigene signature in the CGGA cohort. GBM patient data from TCGA cohort were used for validation. Results: We identified 137 (97 up- and 40 down-regulated) DEGs between GBM and healthy SVZ samples. Enrichment analysis revealed that DEGs were mainly enriched in immune-related terms, including humoral immune response regulation, T cell differentiation, and response to tumor necrosis factor, and the MAPK, cAMP, PPAR, PI3K-Akt, and NF-κb signaling pathways. An eight-gene (BCAT1, HPX, NNMT, TBX5, RAB42, TNFRSF19, C16orf86, and TRPC5) signature was constructed. GBM patients were stratified into two risk groups. High-risk patients showed significantly reduced overall survival compared with low-risk patients. Univariate and multivariate regression analyses indicated that the risk score level represented an independent prognostic factor. High risk score of GBM patients negatively correlated with 1p19q codeletion and IDH1 mutation. Immune infiltration analysis further showed that the high risk score was negatively correlated with activated NK cell and monocyte counts, but positively correlated with macrophage and activated dendritic cell counts and higher PD-L1 mRNA expression. Conclusion: Here, a novel gene signature based on DEGs between GBM and healthy SVZ was developed for determining GBM patient prognosis. Targeting these genes may be a therapeutic strategy for GBM.
Relationship Between Growth Mindset and English Language Performance Among Chinese EFL University Students: The Mediating Roles of Grit and Foreign Language Enjoyment

There is no denying that there is ample evidence of numerous factors that influence language learners' success. Recently, there is a critical call to embrace positive psychology that is more open and appreciative of the positive influences in learning English as a foreign language (EFL). Set against this burgeoning area of study in language learning, this paper puts forward the findings of a study that aimed to examine the mediating roles of grit and foreign language enjoyment in the relationship between growth mindset and English language performance. The study employed a correlational research design involving 388 EFL students from one university in China. The data were collected through a questionnaire and an English language performance test. Using the structural equation modeling, this study found that the association between growth mindset and English language performance was partially mediated by grit and foreign language enjoyment. This indicates that students with a growth mindset tend to possess a higher level of grit as well as experience more enjoyment in learning English, which consequently can lead to students becoming more successful language learners. These findings provide significant implications for language teachers, educational material developers, and school administrators in China to embrace the affective domain postulated by positive psychology.
Research Advances and Prospects of Orphan Genes in Plants

Orphan genes (OGs) are defined as genes having no sequence similarity with genes present in other lineages. OGs have been regarded to play a key role in the development of lineage-specific adaptations and can also serve as a constant source of evolutionary novelty. These genes have often been found related to various stress responses, species-specific traits, special expression regulation, and also participate in primary substance metabolism. The advancement in sequencing tools and genome analysis methods has made the identification and characterization of OGs comparatively easier. In the study of OG functions in plants, significant progress has been made. We review recent advances in the fast evolving characteristics, expression modulation, and functional analysis of OGs with a focus on their role in plant biology. We also emphasize current challenges, adoptable strategies and discuss possible future directions of functional study of OGs.
Interaction Matters: The Effect of Touching the Social Robot PARO on Pain and Stress is Stronger When Turned ON vs. OFF

Social touch between humans, as well as between humans and animals, was previously found to reduce pain and stress. We previously reported that touching a social robot can also induce a reduction in pain ratings. However, it is unclear if the effect that touching a robot has on pain perception is due to its appearance and its pleasant touch, or due to its ability to socially interact with humans. In the current experiment, we aimed to assess the contribution of the interactive quality to pain perception. We assessed the effect of touching the social robot PARO on mild and strong pain ratings and on stress perception, on a total of 60 healthy young participants. The robot either interacted with participants (ON group, n = 30) or was turned off (OFF group, n = 30). Touching the robot induced a decrease in mild pain ratings (compared to baseline) only in the ON group while strong pain ratings decreased similarly in both the ON and the OFF groups. The decrease in mild pain ratings in the ON group was significantly greater in participants with a higher positive perception of the interaction with PARO. We conclude that part of the effect that touching the robot has on pain stems from its interactive features.
“What Other Information Is There?”: Identifying Information Gaps, Perceptions and Misconceptions on COVID-19 Among Minority Ethnic Groups in the Netherlands

Background Multiple media platforms and various resources are available for information on the novel coronavirus disease (COVID-19). Identifying people's preferences is key to building public confidence and planning for successful national or regional health intervention strategies. Methods Using exploratory mixed-methods including a short survey, interviews and participant observation, this cross-sectional study of 160 respondents from the Ghanaian-Dutch, Afro and Hindustani Surinamese-Dutch communities in Amsterdam, the Netherlands was conducted. Data collected between February to April 2021, included demographics characteristics, knowledge, opinions, preferred source of information, behavioral factors, and information gaps on COVID-19 prevention measures, responses and decision-making of respondents. Descriptive statistics and follow-up in-depth interviews were conducted to determine the relationship between respondents' demographics, information sources, and attitudes/behaviors toward COVID-19. Results The findings of this study indicated that although many of the respondents from these communities had good knowledge on COVID-19, its modes of transmission and prevention measures, their willingness to take up initiatives and prioritize self responsibility toward their health are tied to their communal life. The respondents in this study demonstrated high value for social lives and relied on their connections with friends and families in shaping, obtaining, processing and utilizing COVID-19 information to build a sense of responsibility toward the uptake of COVID-19 prevention measures despite recent decline in number of cases. Conclusion This sense of responsibility means their active participation and ownership of interventions to address the specific personal concerns and that of their community. However, different factors play influential roles toward the behavior choices of our respondents regarding the COVID-19 prevention.