Miracle-dz/UniFAR-FTUs huggingface dataset:
S0033291720000562jlp 1..3

Institute of Clinical Medicine/Psychiatry, University of Eastern Finland, Kuopio, Finland; Department of Social Sciences, University of Eastern Finland, Kuopio, Finland; Department of Psychiatry, Kuopio University Hospital, Kuopio, Finland; Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland; Department of Psychiatry, University of Helsinki and Helsinki University Hospital, Helsinki, Finland and Department of Psychology and Logopedics, Faculty of Medicine, University of Helsinki, Helsinki, Finland
Plasminogen Activator in Mouse Melanoma Cells

The decrease of tumorigenicity by mouse melanoma clone B559 after growth in the presence of 5bromodeoxyuridine (BrdU) has been correlated with a decrease in detectable cellular plasminogen activator. Reduction of both activities occurs after one to two cell divisions in the presence of this thymidine analog and is virtually complete within three to four cell cycles. These changes are fully reversible; four to five cell divisions in the absence ofBrdU are sufficient to allow both tumorigenicity and plasminogen activator levels to return to normal. These results support the hypotheses that (a) the expression of a cellular plasminogen activator is closely associated with the transformation of normal to malignant cells and that (b) the suppression of tumorigenicity by BrdU reflects the capacity of this base analog to inhibit the expression of specialized functions which accompany the malignant state. The immediate chemical environment plays a central role in the proliferation and differentiation of the cell. By release of enzymes or other biologically active molecules, the cell may directly influence its own fate or that of neighboring cells. Since there is some evidence that mild proteolysis of the cell surface can transiently affect the surface properties of normal cells (1, 2), the increased levels of proteases in transformed cells (3-7) assume enhanced significance. One such proteolytic activity of transformed cells, the ability of the cells to induce fibrinolysis under appropriate conditions, has been shown to result from the activation of plasminogen by a protease released from the cells (8). Normal cells which do not possess detectable levels of plasminogen activator are converted to plasminogen activator-producing cells by transformation with RNA or DNA viruses or with chemical carcinogens (9, 10). The expression of this enzymatic activity in such cells requires transformation (9), and its appearance is one of the earliest manifestations of the transformed state. The close association of the plasminogen activator with in vitro transformation and its widespread occurrence in tumor tissues and transformed cell lines (10, 11) stimulated us to examine the relationship between tumorigenicity and the presence of this activity. The cell type we have chosen for these studies is the BrdU sensitive clone B559 of mouse melanoma B16 described by Silagi et al. (12, 13). Clone B559 responds to growth in the presence of BrdU with both morphological and biochemical alterations (12-15). The change from highly melanotic cells growing in piled-up, semi-colonial arrays to flat, amelanotic, fibroblastic cells is accompanied by complete suppression of tumorigenicity; but 47 neither the plating efficiency nor the ability of these cells to grow in culture is affected sufficiently to account for these changes (16). The reduction in tumorigenicity and alterations in cell morphology induced by BrdU are reproducibly reversed by removing BrdU from the growth medium (12,
RUNNING HEAD: POLYPHOSPHOINOSITIDE MICRODOMAINS IN PLANT PLASMA MEMBRANE

In this paper, we analyzed the lipid composition of Detergent-Insoluble Membranes (DIMs) purified from tobacco (Nicotiana tabacum) plasma membrane (PM), focusing on polyphosphoinositides, lipids known to be involved in various signal transduction events. Polyphosphoinositides were enriched in DIMs compared to whole PM, whereas all structural phospholipids were largely depleted from this fraction. Fatty acid composition analyses suggest that enrichment of polyphosphoinositides in DIMs is accompanied by their association with more saturated fatty acids. Using an immunogold-electron microscopy strategy, we were able to visualize domains of phosphatidylinositol 4,5bisphosphate (PtdIns(4,5)P2) in the plane of the PM with 60% of the epitope found in clusters of ca. 25 nm in diameter and 40% randomly distributed at the surface of the PM. Interestingly, the PtdIns(4,5)P2 cluster formation was not significantly sensitive to sterol depletion induced by methyl-β-cyclodextrin (mbCD). Finally, we measured the activities of various enzymes of polyphosphoinositide metabolism in DIMs and PM and showed that these activities are present in the DIM fraction, but not enriched. The putative role of plant membrane rafts as signaling membrane domains or membrane docking platforms is discussed. www.plantphysiol. org on September 3, 2017 Published by Downloaded from Copyright © 2010 American Society of Plant Biologists. All rights reserved.
Austin Virology and Retro Virology Xeroderma Pigmentosum Complementation Group D (XPD) Codon 751 and Exonuclease 1 Glu 589 Lysgene Polymorphismsare Associated with Hepatocellular Carcinoma in Egyptian Patients with HCV (Genotype-4)

Aim: Xeroderma Pigmentosum Complementation Group D (XPD) is a major DNA repair gene in Nucleotide Excision repair system. Exonuclease 1 (Exo 1) is an important modulator in mismatch repair system. Our aim is to investigate the association of XPD/ERCC2 Lys751Gln (A/C) (rs 13181) and Exo- 1 Glu 589 Lys (G/A) (K589E) polymorphisms with Hepatocellular Carcinoma (HCC) in Egyptian patients. Methods: Fifty HCC patients and 50 healthy controls were included. Genotyping for XPD/ERCC2 Lys751Gln was performed by Real-Time PCR and for Exo- 1 (K589E) Glu/Lys by PCR- RFLP. Results: Patients with XPD Lys751Gln combined (AC + CC) genotypes had an increased risk of HCC compared to the wild AA genotype ( p = 0.027, OR = 2.47). The mutant C allele frequency was higher in HCC than in controls, ( p = 0.035). Mean serum albumin level was lower in patients carrying (AC + CC) genotypes than in AA genotype ( p = 0.027). Median serum levels of ALT, AFP and creatinine were higher in AA genotype than in combined genotypes ( p = 0.011, 0.007, 0.045 respectively). Exo- 1 Glu 589 Lys (AA) mutant genotype was associated with increased risk of HCC ( p = 0.031). The frequency of the mutant A allele was higher in HCC compared to controls, ( p = 0.044). Serum creatinine in wild type was higher than in other genotypes ( p = 0.034). Conclusion: XPD Lys751Gln and Exo- 1 Glu589 Lys gene polymorphisms are important HCC modulators in Egyptian patients with HCV genotype 4. (A/C) and Exo- 1 Glu 589 Lys (G/A) genes and HCC.
Appendix A : Model description The model description

The purpose of the model is to evaluate two information sources, perception and memory, for zebras making migratory movements from the Okavango Delta to the Makgadikgadi grasslands. In particular, the modelled zebras are free to roam in 2-dimensional space, and we focus on the directional information they may use to locate their destination. The time-scales of the information differs: perception uses current conditions and memory uses long-term average of past conditions. We used remotely sensed vegetation data to represent the habitat in both cases. In the case of the memory mechanism, zebras could transmit migratory information through genetic or cultural means, and our model does not make specific assumpumptions of how the spatial memory is encoded into a cognitive map [3]. Rather, we assume that at some point zebras exhaustively explored their environment and seek to test if memory is important to direct migrating zebras by determining if this information can reproduce observed behaviour.
Effect of water temperature on the morbidity of <fi>Tetracapsuloides bryosalmonae</fi> (Myxozoa) to brown trout (<fi>Salmo trutta</fi>) under laboratory conditions

Proliferative kidney disease (PKD) is a parasitic disease of salmonid fish with a complex life cycle (Okamura et al., 2015). The causative pathogen for this disease is Tetracapsuloides bryosalmonae (Myxozoa: Malacosporea). Its life cycle alternates between bryozoans as main hosts and salmonid fish as intermediate hosts (Anderson et al., 1999). Firstly, the spores are released by the infected bryozoan species, such as Cristatella mucedo, Pectinatella magnifica, Plumatella rugosa, Plumatella emarginata and mainly Fredericella sultana (Bryozoa: Phylactolaemata) (Hartikainen et al., 2014; Longshaw et al., 1999), and enter the fish host through the gills and the skin (Grabner & ElMatbouli, 2010; Longshaw et al., 2002). After invasion, the parasite migrates to the target organ kidney through the bloodstream, where it causes inflammation, eventually leading to an enlargement of the kidney (CliftonHadley et al., 1987; Hedrick et al., 1993; Holzer et al., 2006). In brown trout (Salmo trutta; Linnaeus, 1758) and brook trout (Salvelinus fontinalis; Mitchill, 1814), the parasite forms intraluminal sporogonic stages that are excreted via the urine for further transmission to bryozoan colonies (Hedrick et al., 2004; Grabner Received: 20 November 2020 | Revised: 15 February 2021 | Accepted: 17 February 2021 DOI: 10.1111/jfd.13361
A High-Throughput Arabidopsis Reverse Genetics System

A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal assymetric inter laced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from (cid:2) 100,000 transformed lines. A total of 85,108 TAIL-PCR products from 52,964 T-DNA lines were sequenced and compared with the Arabidopsis genome to determine the positions of T-DNAs in each line. Predicted T-DNA insertion sites, when mapped, showed a bias against predicted coding sequences. Predicted insertion mutations in genes of interest can be identified using Arabidopsis Gene Index name searches or by BLAST (Basic Local Alignment Search Tool) search. Insertions can be confirmed by simple PCR assays on individual lines. Predicted insertions were confirmed in 257 of 340 lines tested (76%). This resource has been named SAIL (Syngenta Arabidopsis Insertion Library) and is available to the scientific community at www.tmri. org.
OP-HURN200185 2097..2106

androgen receptor and have a distinct hormonal responsiveness when compared with endometrial epithelium A. Maclean, E. Bunni, S. Makrydima, A. Withington, A.M. Kamal, A.J. Valentijn, and D.K. Hapangama* Department of Women’s and Children’s Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK Liverpool Women’s NHS Foundation Trust, Member of Liverpool Health Partners, Liverpool L8 7SS, UK Pathology Department, Oncology Teaching Hospital, Baghdad Medical City, Baghdad, Iraq
DMSO_A_256223 1933..1942

Yu-Cheng Cheng 1 Yu-Hsuan Li Chiann-Yi Hsu 3 I-Te Lee 1,4–6 1Division of Endocrinology and Metabolism, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan; 2Graduate Institute of Data Science, Taipei Medical University, Taipei, Taiwan; 3Biostatistics Task Force of Taichung Veterans General Hospital, Taichung, Taiwan; 4School of Medicine, National Yang-Ming University, Taipei, Taiwan; 5School of Medicine, Chung Shan Medical University, Taichung, Taiwan; 6College of Science, Tunghai University, Taichung, Taiwan Purpose: Carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) are tumor-associated antigens. An increased serum level of CEA and CA19-9 separately has been reported in diabetes. In this study, we examined the composite effect of elevated serum levels of both CEA and CA19-9 on subjects with type 2 diabetes and prediabetes. Patients and Methods: A total of 3568 adults who attended a health examination were enrolled into this cross-sectional study. Subjects were grouped into four groups according to the median serum CEA and CA19-9 levels. Results: Subjects with high CEA and high CA19-9 levels had the highest proportions of diabetes (43.9%) and prediabetes (33.04%). There was a statistically significant trend in the proportion of diabetes across the four groups (P < 0.001). Multivariable logistic regression analysis revealed higher risks of type 2 diabetes in subjects with high CEA and low CA19-9 levels (odds ratio [OR] = 2.10, 95% confidence interval [CI]: 1.39–3.18, P < 0.001) and in those with high CA19-9 and low CEA levels (OR = 2.18, 95% CI: 1.42–3.34, P < 0.001) than in those with low CEA and low CA19-9 levels; among these four groups, the highest risk of type 2 diabetes was observed in subjects with high CEA and high CA19-9 levels (OR = 2.65, 95% CI: 1.81–3.88, P < 0.001). The risk of prediabetes was significantly higher only in subjects with high CEA and high CA19-9 levels compared to those with low CEA and low CA19-9 levels (OR = 1.32, 95% CI: 1.08–1.61, P = 0.006). Conclusion: CEA and CA19-9 had a synergistic ability to increase the risk of type 2 diabetes and prediabetes.
Hide, Don’t Seek: on User-Centric Location Accuracy

—Location Privacy has been a very attractive topic for the past years. The amount of location blurring, hiding and privacy preserving solutions proposed by the researchers is very big. The number of such services implemented on the market, however, is quite small. Moreover, the ones actually used by the people is close to zero. We see this gap as very troubling. With the spread of devices that will allow for tracking users - the smartwatches, connected medical devices, smartphones and ﬁtness trackers, location privacy will become even more important. When the devices become connected into the Internet of Things, so that it will be hardly possible to escape tracking: already today 70% of users sleep with their phones next to their beds. In our work, we approached the problem from a user-centric perspective, developed our solution together with users and learned what are their expectations towards such a feature.
PHOTOLUMINESCENCE OF TAUTOMERIC FORMS OF NANOPARTICLE ENSEMBLES OF DYES BASED ON THE 4-VALENCE STANNUM COMPLEXES IN POROUS SILICA GLASS

Luminescence of tautomeric forms of dyes based on the 4-valence stannum complexes was researched. Symmetry of photoluminescence spectra of different tautomeric forms relative to direction of reading of atom positions in the hydrazide fragment was found clockwise or anti clockwise. It was determined that the illumination of nanoparticle ensembles of dyes in A-type porous silica glass is always more intensive than in appropriate solution. It was shown that the luminescence intensity of tautomeric forms increases, if the substituent comes nearer to the coordination set irrespective of type and nature of substituent and type of coordination set. At the same time, change of illumination energy of tautomeric forms depends on both type and nature of substituent and coordination set. The results were explained by the development of inner surface of matrix and also by the features of atom disposition in dye molecule and by interaction between them.
Essays on Economic Integration

Essays on Economic Integration Jaeho Jung Department of Economics The Graduate School Seoul National University This dissertation consists of two chapters that account for the effects of economic integration between countries. Chapter I analyzed the causality between trade intensity and business cycle synchronization, and Chapter II captured the dynamic interdependence of stock returns. Chapter I empirically researched whether the trade intensity, which was measured by value-added, could significantly explain the output comovement. Three types of trade intensities were constructed by using domestic value-added, total domestic value-added, and net returned domestic value-added, respectively. Using these, this dissertation conducted the empirical analyses based on Ordinary and Two Stage Least Squares for 43 countries from all over the world between 2000 and 2014. The main findings are summarized as follows. First, none of the trade intensities significantly explained the business cycle synchronization. Second, two intra-industry trades, which were constructed by the domestic value-added and total domestic value-added, significantly accounted for the output comovement. Third, the net returned domestic value-added was not important from the empirical point of view. In conclusion, the significant value-added trade channel on business cycle synchronization was not the trade intensity, but the intra-industry trade.
OTT_A_289337 481..489

*These authors contributed equally to this work Purpose: Follicular lymphoma (FL) is an indolent, yet generally incurable neoplasia with a median survival exceeding 10 years. However, a subset of FL patients experiences histological transformation (HT) to a more aggressive lymphoma, in the majority of cases to diffuse large B-cell lymphoma (DLBCL). This affects both the clinical course and the prognostic outcome, resulting in a markedly reduced survival after transformation. Thus, early risk stratification and prediction of patients at risk of HT would be highly valuable in the clinical setting. Here, we investigated the potential of the immune inhibitory programmed death 1 (PD-1) receptor as a biomarker predictive of HT. Patients and Methods: Immunohistochemical staining and quantification by digital image analysis of PD-1 was performed on diagnostic tumor-tissue samples from FL patients with and without subsequent transformation (n=34 and n=46, respectively), and on paired samples from the transformed lymphoma (n=34). Results: At the time of initial FL diagnosis, samples from patients with subsequent HT had significantly higher tumor-tissue expression of PD-1 compared with diagnostic FL samples from patients without subsequent HT (p=0.010). At the time of transformation, PD-1 expression was significantly reduced (p<0.001). No difference was observed in intrafollicular PD-1 expression at FL diagnosis between samples from patients with or without HT; however, high intra-follicular levels of PD-1 were associated with significantly shorter transformation-free survival times (p<0.043). Conclusion: Our data suggest that pre-treatment tumor-tissue PD-1 expression already predicts the risk of subsequent transformation to DLBCL, as early as the time of FL diagnosis.
Interaction of Halogenated Anesthetics with-and -Adrenoceptor Stimulations in Diabetic Rat Myocardium

Background: Halogenated anesthetics potentiate the positive inotropic effects of and -adrenoceptor stimulations. Although diabetes mellitus induces significant myocardial abnormalities, the interaction of halogenated anesthetics and adrenoceptor stimulation in diabetic myocardium remains unknown. Methods: Left ventricular papillary muscles were provided from healthy and streptozotocin-induced diabetic rats. Effects of 1 minimum alveolar concentration halothane, isoflurane, and sevoflurane on the inotropic and lusitropic responses of (phenylephrine)– and (isoproterenol)–adrenoceptor stimulations were studied at 29°C with 12 pulses/min. Data shown are mean percentage of baseline active force SD. Results: Phenylephrine induced comparable positive inotropic effects in healthy and diabetic rats (143 8 vs. 136 18%; not significant), but the potentiation by halogenated anesthetics was abolished in the diabetic rats (121 20, 130 20, and 123 20% for halothane, isoflurane, and sevoflurane, respectively; not significant). In diabetic rats, the positive inotropic effect of isoproterenol was markedly diminished (109 9 vs. 190 18%; P < 0.05), but its potentiation was preserved with isoflurane (148 21%; P < 0.05) and sevoflurane (161 40%; P < 0.05) but not with halothane (126 16%; not significant). Halothane induced a deleterious effect on the sarcoplasmic reticulum, as shown by its impairment in the lusitropic effect of isoproterenol, compared with isoflurane and sevoflurane. Conclusion: Potentiation of the positive inotropic effect of -adrenoceptor stimulation by halogenated anesthetics is abolished in diabetic rats. In contrast, potentiation of -adrenoceptor stimulation is preserved with isoflurane and sevoflurane but not with halothane, probably because of its deleterious effects on sarcoplasmic reticulum.
EFFECTS OF DIFFERENT SCHOOL LOCATIONS AND SIZES ON PHYSICAL ACTIVITY LEVELS AMONG THAI SECONDARY-SCHOOL STUDENTS

Jirachai Karawa Satian Laoprasert Suparat Karawa Teerachpan Maneetam and Kurusart Konharn 1 Faculty of Management Sciences and Information Technology, Nakhon Phanom University 2 Exercise and Sport Sciences Program, Graduate School, Khon Kaen University 3 Faculty of Education, Mahasarakam University 4 School of Physical therapy, Faculty of Associated Medical Sciences, Khon Kaen University 5 Research Center in Back, Neck, Other Joint Pain and Human Performance (BNOJPH), Khon Kaen University Received: 22 November 2019 / Revised: 24 February 2020 / Accepted: 5 March 2020
Kinetics of A 0 Binding to DNP of Lymphocytes in Allogeneic Mixed Cultures

Allogeneic lymphocytes mixed in vitro ("mixed cultures") undergo a series of changes characterized by increased synthesis of RNA, protein, and DNA resulting in morphological transformation (1-3), provided that the lymphocytes originate from individuals who are incompatible at the major human histocompatibility locus. Synthesis of DNA occurs 2-3 days after mixing and mitoses appear from the 3rd to the 5th day. Similar cellular changes occur in lymphocytes stimulated by phytohemagglutinin (PHA)* (4). This growth stimulating effect of PHA has been investigated by means of cytophotometric techniques (5-7). Fluorescence measurements were carried out on individual fixed leukocytes stained with acridine orange (AO) which binds to the negatively charged phosphate groups in nucleoproteins (8). A few minutes after administration of PHA, a pronounced increase in fluorescence intensity was found, due to an increased AO binding to the deoxyribonucleoprotein (DNP) complex of the stimulated cells. No change in the cellular amounts of nucleic acids occurred during this time (5, 6). I t was concluded that the increased AO binding was due to an unscreening of previously masked phosphate groups in DNA, most probably as a result of weakened bonds between the DNA and the protein moiety in the DNP complex. Such DNP changes might be of importance for the later synthesis of RNA and blast transformation (5-7).
CMAR_A_292197 1169..1177

Department of Radiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, People’s Republic of China Background: To evaluate the predictive value of the OATP1B3 expression in hepatocellular carcinoma (HCC) for the gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (GdEOB-DTPA) uptake and the signal intensity (SI) in the hepatobiliary (HB) phase. Methods: In this retrospective study, we analyzed 69 liver nodules of 64 patients who underwent Gd-EOB-DTPA enhancement magnetic resonance imaging (MRI) before operation. Based on the SI in the HB phase, the patients were categorized into the hypointense HCC and isoor hyperintense HCC groups. The OATP1B3 expression was detected by polymerase chain reaction (PCR) and immunohistochemistry. The differences between the expression of OATP1B3 and Gd-EOB-DTPA enhanced magnetic resonance imaging between the two groups of hepatocellular carcinoma were compared. The relationship between the OATP1B3 expression and the SI and relative enhancement (RE) was analyzed. Results: The examined HCC nodules were 59 hypointense HCC and 10 isoor hyperintense. The relative expressions of OATP1B3, HB-phase signal, and the RE of the HB phase in isoor hyperintense were significantly higher than those of the hypointense HCC, while the RE of the HB phase increased with an increase in the OATP1B3 expression (P < 0.05). Conclusion: The OATP1B3 expression in HCC can predict the uptake of Gd-EOB-DTPA and the SI of the HB phase. We believe that the evaluation of OATP1B3 expression will facilitate the comprehension of imaging performance of HCC in Gd-EOB-DTPA-enhanced MRI.