Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification de nouveaux biomarqueurs permettant la caractérisation des lymphocytes T CD8 mémoires innés

Deux grandes classes de cellules composent le pool de lymphocytes T (LT) CD8 memoires. D'une part, les LT CD8 memoires conventionnels sont generes via la reconnaissance specifique d'antigenes derives de pathogenes ou de tumeurs. D'autre part, les LT CD8 memoires innes sont generes via differents mecanismes impliquant de fortes stimulations par des cytokines γc independamment de la reconnaissance d'antigenes du non soi. Le phenotype extremement similaire de ces deux populations cellulaires ne permet pas de les distinguer in vivo. En consequence, la population de LT CD8 memoires innes est relativement peu caracterisee. Mon travail de these comportait donc deux objectifs majeurs : 1 / Identifier des marqueurs permettant de distinguer in vivo ces deux classes de LT CD8 memoires. 2/ Caracteriser la population de LT CD8 memoires innes. Dans cette etude, nous demontrons qu'au sein du pool de LT CD8 memoires, seules les cellules conventionnelles expriment la chimiokine CCL5 et le recepteur NKG2D. Ces deux biomarqueurs permettent ainsi pour la premiere fois de distinguer les LT CD8 memoires innes et conventionnels in vivo, a la fois chez la souris et chez l'homme. Grâce a l'expression de NKG2D, nous demontrons que ces LT CD8 memoires innes possedent des caracteristiques typiques de cellules memoires, notamment une reactivite augmentee ainsi qu'un programme genetique comparable a celui des LT CD8 memoires conventionnels. Neanmoins, cette population cellulaire conserve certaines caracteristiques de cellules naives. Ainsi, le repertoire TCR diversifie de cette population cellulaire permet a ces cellules de participer a des reponses immunitaires primaires contre differents pathogenes. Enfin, dans un contexte inflammatoire, les LT CD8 memoires innes presentent un defaut d'acces au tissu pulmonaire compare aux LT CD8 memoires conventionnels. Ceci correle avec un deficit d'expression de certaines integrines par les LT CD8 memoires innes. L'ensemble de nos resultats demontre que les LT CD8 memoires innes, caracterises par l'absence d'expression de CCL5 et NKG2D, constituent une population cellulaire hybride, a la frontiere entre cellules naives et cellules memoires conventionnelles
Early protection against Salmonella infection in chickens by modification of the initial host-pathogen interactions

S IN INTERNATIONAL MEETINGS : De Bruyker, V. , Ducatelle, R. , Haesebrouck, F. , De Buck, J., De Smet, I. and Van Immerseel, F. (2000). Monitoring the Salmonella carrier state in poultry by pilocarpine injection, Xth International Congress on Animal Hygiene Maastricht, The Netherlands. Ducatelle, R. , De Brucker, V. , De Smet, I. , De Buck, J., Van Immerseel, F. and Haesebrouck, F. (2000). An experimental model for the Salmonella enteritidis carrier state in replacement pullets, XXI World’s Poultry Congress, Montréal, Canada. De Buck, J., Van Immerseel, F. , Meulemans, G. , Haesebrouck, F. and Ducatelle, R. (2002) Interaction of Salmonella Enteritidis with chicken isthmal glandular secretory cells. 20 meeting of the European Society for Veterinary Pathology, Grugliasco, Italy. De Buck J, Van Immerseel F, Haesebrouck F, Ducatelle R. (2003). Protection of laying hens against Salmonella Enteritidis by feed supplementation with tea extracts, guar bean fibers and iodination of the drinking water. XIIIth Congress of the World Veterinary Poultry Association, Denver, Colorado, USA. De Buck J, Van Immerseel F, Haesebrouck F, Ducatelle R. (2003). Prolonged bacteremia, modified genital tract infection and reduction of egg contamination by a type 1 fimbriae mutant of Salmonella Enteritidis. XIIIth Congress of the World Veterinary Poultry Association, Denver, Colorado, USA. Van Immerseel, F. , Meulemans, L. , De Buck, J., Pasmans, F. , Haesebrouck, F. and Ducatelle, R. (2003). Early protection against Salmonella Enteritidis after vaccination
Nosema Apis in Drone Honeybees

SummaryDrone honeybees were found to be at least as susceptible as workers to infection by Nosema apis, and the median infective dose was fewer than 100 spores. Nevertheless proportionately many fewer drones than workers became infected in enzootically infected, undisturbed honeybee colonies. This suggests that infection does not spread primarily via food and water, although the comparatively few drones that become infected may receive spores from the mouthparts of comb-cleaning worker bees when they obtain food from these.
Macrophytes as indicators and potential remediators in aquatic ecosystems : A case study

The content of macronutrients (N, P, K, Na) and heavy metals (Fe, Mn, Zn, Cu, Ni, Pb, Cd, Co) in plant samples of dominant aquatic macrophytes (Ceratophyllum demersum, Phragmites australis, Trapa natans, Hydrocharis morsus-ranae, Nymphaea alba, Nymphoides peltata, Salvinia natans) from the river Jegricka (Serbia) is presented. Macronutrients content in plant tissue was above necessary limits for plant maximum growth indicating to substantial bioavailability of those elements typical for eutrophic environment. Content of investigated nutrients and heavy metals varied in relation to plant species. Ceratophyllum demersum turned out to be best bioaccumulator for P, K, Fe, Mn, Cu, Ni, Pb, and Co. Salvinia natans accumulated high amounts of N, P, K, Na, and especially Cu and Zn. Nymphaea alba accumulated highest recorded amounts of N and Na. Hydrocharis morsus-ranae accumulated very high amounts of N, P, K, Na, Pb, and Mn. Because of its fast growth, large abundance and homogeneous distribution on this locality, Phragmites australis might have high potential in use for phytoextraction of macronutrients and heavy metals. The application of macrophytes as indicators might be limited depending on the varying of environmental conditions on different localities.
An Improved Shoot Regeneration System for Somaclone Production in Tomatoes

Efficiency of somaclone production was examined using 10 processing tomato cultivars and 18 growth regulator treatments. Although zeatin has been reported to induce shoot development most readily, a combination of IAA (5.7 µmol·liter–1) and kinetin (4.7 µmol·liter–1) was found to be equal to zeatin in shoot regeneration efficiency and reduced the time required for shoot production. Rooting was achieved by transplanting the in vitro-produced shoots to Pro-Mix under intermittent mist in a greenhouse. Chemical names used: (E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin); 1H-indole-3-acetic acid (IAA); N-1-furanyImethyl)-1-H-purin-6-amine (kinetin).
First report of a rickettsia-like organism from farmed Chinook salmon, Oncorhynchus tshawytscha (Walbaum), in New Zealand

ABSTRACT In the summer months of 2015 Chinook salmon, Oncorhynchus tshawytscha (Walbaum), were submitted to the New Zealand Ministry for Primary Industries (MPI) for disease investigation. Gross examination of 10 moribund fish revealed multiple areas of ulceration over all areas of the body including fins and mouth in eight of the 10 fish. Pale livers (n = 4), mottled appearance of the kidney (n = 5) and reddening of the distal intestines (n = 5) were also observed. As these fish presented with skin lesions, Piscirickettsia salmonis was included as a differential diagnosis. Testing for this agent was conducted and a rickettsia-like organism (RLO) was detected by histopathology in seven fish and by molecular methods in three fish. Nucleic acid sequencing revealed that this organism had highest similarity to a piscirickettsia-like organism with a 19 base pair (bp) deletion in the internal transcribed spacer (ITS) region of the rDNA operon when compared to the reference strain P. salmonis, LF-89 (ATCC VR-1361). The variation was similar to that of the Tasmanian RLO detected in Atlantic salmon, Salmo salar L (GenBank accession number AY578985). Based on these sequence variations, a tentative identification of New Zealand rickettsia-like organism (NZ-RLO) was suggested. NZ-RLO is not considered to be the primary cause of the mortalities in these fish, with the primary cause still under investigation. This is the first report of a RLO in New Zealand farmed salmon.
Liver function test in diabetic patients in Punjabi population

Diabetes mellitus is a common endocrine metabolic disorder characterized by hyperglycemia. The liver plays a major role in the regulation of carbohydrate metabolism, as it uses glucose as a fuel. This key function of liver makes it vulnerable to diseases in subjects with metabolic disorders, particularly diabetes. The present study aims to study the prevalence of liver enzymes Aspartate Transaminase (AST) and Alanine Transaminase (ALT) in Diabetic subjects from Punjabi population. In order to meet the proposed objectives of this study, whole blood samples were collected from 100 diabetic and 100 non diabetic subjects. Each sample was analysed for Fasting plasma glucose (FPG), HbA1C, AST and ALT. When categorized on the basis of type of disease 20% were of type 1 diabetes mellitus and 80% were of type 2 diabetes mellitus. In our study liver enzymes i.e. AST (p=0.0007) and ALT (p=0.0005) are significantly higher in diabetes compared to non diabetes subjects. Type 1 diabetic subjects did not shows any significant difference in the level of AST (p=.468) and ALT (p=0.978) compared to non-diabetic subjects, where as type 2 diabetic subjects shows significant higher level of AST (p=<.0001) and ALT (p=<.0001) compared to non-diabetic subjects. Failure to recognize the presence of abnormal liver enzymes may be a primary cause of poor management often encountered in some of the treated diabetics. There is therefore need for the routine assay of liver profile in diabetic, particularly in type 2 patients whose conditions are difficult to manage.
THE EFFECTS OF COOKED COMMON BEANS ON DSS-INDUCED COLITIS IN MICE

THE EFFECTS OF COOKED COMMON BEANS ON DSS-INDUCED COLITIS IN MICE Penglihui Claire Zhang Advisor: University of Guelph, 2012 Dr. Kelly Meckling This thesis is an investigation of the effects of whole beans, with different levels of phenolics level, on colonic inflammation in mice. C57BL/6 male mice were fed a basal diet (BD) supplemented with or without 20% bean flour for 3 weeks. Colitis was induced by 2% dextran sulphate sodium (DSS) in mice drinking water. Phenolic determinations of the bean flours revealed that within each pair of beans, the darker bean had higher phenolic content and antioxidant activity compared to the lighter one. In addition, only coloured beans had anthocyanin. When bean were consumed both prior to and during DSS cycle, the mice experienced both disease attention and aggravation. However, when bean consumption was limited to prior to DSS induction only, mice had reduced colitis upon later DSS induction. Overall, findings suggest that the effects of bean may differ depending on the timing of consumption.
The implications of asymmetric dispersal for metapopulation dynamics and conservation

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy THE IMPLICATIONS OF ASYMMETRIC DISPERSAL FOR METAPOPULATION DYNAMICS AND CONSERVATION By Miguel A. Acevedo May 2013 Chair: Robert J. Fletcher, Jr Major: Interdisciplinary Ecology Variations in movement have broad implications for many areas of ecology and evolution. For instance, in metapopulation theory, movement plays an important role promoting (meta)population persistence over time. Most metapopulation modeling approaches describe patch connectivity using pair-wise Euclidean distances resulting in the simplifying assumption of a symmetric connectivity pattern. Symmetric connectivity may be rarely observed in nature where organisms move responding to environmental cues or advection sources through heterogeneous landscapes. Assuming symmetric dispersal when movement is asymmetric may result in biased estimates of colonization, extinction and persistence, which has important implications for management and conservation. Here I studied the implications of asymmetric connectivity for patch colonization and extinction. I leveraged a long-term, time-series on colonization-extinction dynamics in the wind-dispersed orchid Lepanthes rupestris and found that models that accounted for these asymmetries had better fit. The implications of asymmetric connectivity to metapopulation dynamics may be contingent on the driving mechanism behind them. I used a combination of an observational study of individual movements and translocation experiments to test for possible mechanisms of directed movement in the cactus-feeding insect, Chelinidea vittiger and found that variations in patch size were the most important driver of directed movements in this species. I also developed
Solubility, functional properties, ACE‐I inhibitory and DPPH scavenging activities of Alcalase hydrolysed soy protein hydrolysates

Summary Soy proteins are less soluble at acidic pH value, which impedes their utilisation in acidic beverages. Soy protein isolate (SPI) was hydrolysed using varying Alcalase concentrations (0.0001–2.0 U g−1 protein) at different pHs (3.0–4.0). Degree of hydrolysis (DH) of soy protein hydrolysates (SPH) at pH 3.0, 3.5 and 4.0 were 5.0–10.7%, 2.3–6.1% and 0–5.4%, respectively, while solubilities ranged from 70.7 to 74.9%, 18.8 to 51.2% and 7.1 to 40.4%, respectively. The highest solubility (74.9%) was observed at pH 3.0 with 1.5 U Alcalase per g protein (DH = 9.2%). Emulsifying activities of SPHs at pH 3.0 and 4.0 ranged from 0.49 to 0.63 AU and 0.19 to 0.24 AU, respectively, while the emulsifying stabilities were 12.2–14.7 min and 18.7–56.0 min, respectively. The foaming capacity at pH 3.0 and 4.0 was 44.9–46.3 mL and 31.2–41.3 mL, respectively, whereas the foaming stability was 25.5–35.2 min and 12.8–15.1 min, respectively. However, hydrolysates had an insignificant effect on ACE-I inhibitory and DPPH scavenging activities in comparison with SPI.
Component Analysis of Acorns of Quercus mongolica and Quercus Variabilis

To compare seed components of plus trees, seed powder ground after seedcoat removal was analyzed for two oak species, i. e. , Quercus monglica (white oak) and Quercus variabilis (red oak), which are typical oak trees in Korea but have different fruiting characteristics. Thus we aimed at analyzing and comparing many ingredients including minerals, sugars, etc. Two species were similar to each other in the content of water, crude ash, crude protein and carbohydrates, but crude lipid content in Q. variabilis was 2.5 times higher than that in Q. mongolica. Crude proteins of Clone 124 was 1.5 times higher than that of Clone 75 in Q. mongolica. Crude lipid content showed the highest value in Clone 0511 of Q. variabilis, and more phosphate and iron was found in Q. monglica than in Q. variabilis. Glucose showed 85.4% and 88.3% on average of the total monosacchrides in two species, and galactose and arabinose were also found. In the content of phosphate, iron, and crude lipid, differences were found between two species and among clones of two species.
Choosing native tree species for establishing man-made forest: A new perspective for sustainable forest management in changing world

Subiakto A, Rachmat HH, Sakai C. 2016. Choosing native tree species for establishing man-made forest: A new perspective for sustainable forest management in changing world. Biodiversitas 17: 620-625. Establishment of tree plantation on degraded lands and forest clearly favored some exotic species such as Gmelina arborea, Acacia, and Eucalyptus. High productivity, less harvesting time, and deeper silvicultural knowledge are the beneficial factor for choosing those exotics species. However, the use of a wide variety of native tree species becomes more significantly important in reforestation projects due to the greater biodiversity benefits and wider environmental services. This research was carried out as a multiyear observation and continuous experiment to value how native tree species can be prospective alternatives in providing and supporting human need. The performances of two native Indonesian Shorea species, Shorea leprosula and Shorea selanica, were evaluated at a dipterocarp planting trial in two different sites in Indonesia. Growth data was obtained from 15 and 17 years old plots, twelve 100 m X 100 m square plots on mineral soils (Gunung Dahu Experimental Forest/GDEF, Bogor) and eight resembled plots on frequently flooded peat land (PT. Arara Abadi/PT. AA, Riau). Survival rates were varied, ranged from 36-77%, diameter at breast height from 13.7-24.9 cm, tree height from 10.8-16.9 m, mean volume from 0.119 m/tree-0.567 m/tree, and total volume from 79.420 m/ha-215.412 m/ha. Growth rates of planted saplings were affected by species, site and spacing distance. The development of man-made dipterocarps forest in the tropic, especially in South East Asia can be as prospective as developing an exotic fast growing plantation. Eventhough in situ conservation would give the most benefit in conserving genetic resources of native tree species, establishing man-made dipterocarp forest still have higher environmental value than using exotic tree species such as acacia. Moreover, establishing man-made dipterocarp forest is considered more environmental friendly and possess lower to no risk of species invasion compare to those of developing exotic trees plantation.
Genetic diversity analysis of 60 ginger germplasm core accessions using ISSR and SSR markers

Abstract Molecular techniques play a critical role in studies of phylogeny and, thus, have been applied to understand the distribution and extent of genetic variation within and between species. In the present study, a genetic analysis was undertaken using molecular markers (9 ISSR and 13 SSR) on 60 ginger cultivars from different regions of the eastern coast of India (Odisha). The data obtained with 22 polymorphic markers revealed moderate to high diversity in the collection. Both ISSR and SSR markers were efficient in distinguishing all the 60 ginger cultivars. A total of 42 and 160 polymorphic bands were observed with ISSR and SSR markers, respectively. However, SSR markers were observed to be better at displaying average polymorphism (63.29%) than ISSR markers (55%). Analysis of molecular variance results showed that 52 and 66% of the variation occurred among different ginger populations, whereas 48 and 34% of the variation was found within populations, respectively, using ISSR and SSR markers, indicating that ginger cultivars display significant genetic diversity at the population level. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of ginger accessions to their respective area of collection, indicating geographical closeness due to genetic similarity irrespective of the relationship that exists at the morphological level.
Effect of nitrogen and phosphorus levels on nodulationand yield components in faba bean (Vicia faba L.)

The objective of this research was to investigate different levels and the combined effect of nitrogen and phosphorus on the nodulation and yield components of faba bean (Vicia faba L.). A large seeded 584-066 accession numbered faba bean line, which was taken from a breeding program of the Field Crops Department, was used in the experiment. Four different nitrogen (0, 30, 60, 90 kg/ha) and three phosphorus (0, 40, 80 kg/ha) levels were applied to the soil at the sowing time. The number of pods/plant, biological and grain yield per unit area, together with grain and plant N content, were increased by 60 kg/ha nitrogen combined with 40 kg/ha phosphorus applications. The highest nodule and nodule weight/plant and 100-kernel weight were obtained from 80 kg/ha phosphorus and 30 kg/ha nitrogen combination.
Revegetation Trials on a Saltgrass Meadow

Highlight: Saltgrass (Distichlis strictu (Torr.) Rydb. ) meadows may be converted to productive pasture if the low-value saltgrass is removed and productive forage plants established. Field planting trials were conducted in 1975 and 1976 to determine the effects of nitrogen fertilizer, chemical soil amendments, mulch, and furrows on establishment of crested wheatgrass (Agropyron desertorum (Fisch. ex Link) Schult. ), tall wheatgrass (A. elonguturn (Host) Beauv. ), Russian wildrye (Elymus junceus Fisch.), and smooth brome (Bromus inermis Leyss.). In 1975, alternate-furrow planting produced thinner stands than nonfurrowed planting, but in 1976, yields of the 1975 planting were similar from both treatments. Nitrogen fertilizer did not improve stand establishment or yield. All species became established, but tall wheatgrass appeared to be least able to withstand the droughty conditions encountered in late 1975 and all of 1976. In the 1976 planting, a straw mulch increased seedling height and vigor but did not increase the number of seedlings or stand ratings. Because of its combined high seedling vigor and good drought tolerance, crested wheatgrass produced significantly better stands than did the other three species in the 1976 planting. (McGinnies et al. 1976). Soil reclamation treatments on drylands, such as fertilization, water catching and storage, chemical or organic amendments and deep tillage, have increased seeding success on saline-sodic soils in other locations (Soiseth et al. 1974). In a greenhouse study using Natrustoll soils from various horizons, biomass yields of both the aboveand below-ground plant parts of tall wheatgrass were lower on the C horizon and on a mixture of A, B, and C horizons than on A or B horizons (McGinnies and Ludwig 1977). It was concluded from this study that one should avoid plowing into the C horizon because of its high salt level, but the A and B horizons should be mixed by plowing to facilitate water infiltration and increase availability of nutrients. It was also concluded that nitrogen fertilizer increased biomass and improved infiltration under greenhouse conditions. The objectives of the field research reported here were to extend the greenhouse experiment to a field environment, to find palatable grass species adapted to Natrustoll soils, to evaluate the effects of N and Ca on seedling establishment, and to develop methods for establishing these species on Natrustolls under semiarid climatic conditions. Saltgrass (Distichlis stricta (Torr.) Rydb. ) meadows, found extensively on lowland sites throughout the western United States, are of little value for livestock production. They are more moist than adjacent upland sites and have high potential for forage production if palatable salt and alkali tolerant grasses can be established (Plummer et al. 1955). The unpalatable saltgrass is difficult to eradicate because of its persistent rhizomatous growth. Mechanical treatments are usually not successful, but saltgrass has been successfully eradicated by spraying with glyphosate (McGinnies 1974). Methods