Miracle-dz/UniFAR-FTUs huggingface dataset:
Cardio-Hypothalamic-Pituitary Coupling during Rest and in Response to Exercise

The objective of this study was to examine cardio hypothalamic-pituitary coupling and to better understand how the temporal relations between these systems are altered during rest and exercise conditions. An intensive within subjects study design was used. Seven adult males completed two visits, each consisting of either a 24 h period of complete rest or a 24 h period containing a high-intensity exercise bout. An intravenous catheter was used to collect serum samples every 10 min throughout the 24 h period (i.e., 145 samples/person/condition) to assess growth hormone (GH) dynamics throughout the 24 h period. Cardiac dynamics were also collected throughout the 24 h period and epoched into 3 min windows every 10 min, providing serial short-time measurements of heart rate variability (HRV) concurrent to the GH sampling. The standard deviation of the normal RR interval (SDNN), the root mean square of successive differences (rMSSD), and sample entropy (SampEn) was calculated for each epoch and used to create new profiles. The dynamics of these profiles were individually quantified using SampEn and recurrence quantification analysis (RQA). To address our central question, the coupling between these profiles with GH was assessed using cross-SampEn and cross-RQA (cRQA). A comparison between the epoched HRV profiles indicated a main effect between profiles for sample entropy (p < 0.001) and several measures from RQA. An interaction between profile and condition was observed for cross-SampEn (p = 0.04) and several measures from cRQA. These findings highlight the potential application of epoched HRV to assess changes in cardiac dynamics, with specific applications to assessing cardio hypothalamic-pituitary coupling.
Estimation of fluoride uptake in soil and staple food crops produced in highly fluoridated and non-fluoridated regions of Raichur District, Karnataka

Background and Aim: A staple food crops have varied role in diet of people living in particular regions of world; hence, it is critical to recognize their productivity. Therefore, the purpose of this study was to estimate fluoride concentration in staple food crops grown in highly fluoridated and non-fluoridated regions and its correlation with soil. Method: Total 36 samples were collected of which 18 samples consisting of each three samples of rice, redgram and jowar were selected. Likewise 18 corresponding soil samples from both areas were collected. All samples were ashed for 4–6 hours at 550°C in muffle furnace. The samples were allowed to cool, after which 10 ml distilled water was added to each sample and fluoride concentration was determined using ion selective electrode method, before each sample analysis the instrument was standardized using fluoride containing TISAB (III) buffer solution. The data was tabulated and subjected to cross-sectional observational statistical analysis using SPSS software applying unpaired t-test and Pearson’s test. Result: The mean fluoride concentration in crops and soils were rice (0.79 ppm), redgram (4.26 ppm), jowar (8.8 ppm) and in soil of rice (1.23 ppm), redgram (1.23 ppm) and jowar (1.21 ppm) respectively in fluoridated area. Where as in non-fluoridated area rice (0.07 ppm), redgram (0.81 ppm), jowar (0.81 ppm) and in soil of rice (0.61 ppm), redgram (0.07 ppm) and jowar (0.52 ppm) respectively. The resultant correlation between staple food crops with their corresponding soils were found highly significant in both regions with P value <0.005; hence, crops in fluoridated region exhibited increased fluoride retention, whereas crops in non-fluoridated region had optimal fluoride levels. Conclusion: Fluoride concentration in food crops has strong correlation with their respective soils and water irrigation properties.
The Dual Roles of Nano Zero-Valent Iron and Zinc Oxide in Antibiotics Resistance Genes (ARGs) Spread in Sediment

Nanoparticles (NPs) are widely used and ubiquitous in the environment, but the consequences of their release into the environment on antibiotics resistance genes (ARGs), microbial abundance, and community, are largely unknown. Therefore, this study examined the effect of nano zero-valent iron (nZVI) and zinc oxide (nZnO) on tetracycline resistance genes (tet-ARGs) and class 1 integron (intI1) in sediment under laboratory incubation. The coexistence of NPs and tetracycline (TC) on tet-ARGs/intI1 was also investigated. It was found that nZVI and nZnO promoted tet-ARGs/intI1 abundance in sediment without TC but reduced the inducing effect of TC on tet-ARGs/intI1 in sediment overlaid with TC solution. Without TC, nZVI, intI1, and the bacterial community could directly promote tet-ARGs spread in nZVI sediment, while intI1 and bacterial abundance were the most directly important reasons for tet-ARGs spread in nZnO sediment. With TC, nZVI and bacterial community could reduce tet-ARGs abundance in nZVI sediment, while nZnO and bacterial community could directly promote tet-ARGs in nZnO sediment. Finally, these findings provided valuable information for understanding the role of NPs in promoting and reducing ARGs in the environment.
Antibiotic and Nematocidal Metabolites from Two Lichen Species Collected on the Island of Lampedusa (Sicily)

The antibiotic and nematocidal activities of extracts from two coastal lichen species collected on Lampedusa Island (Sicily), Ramalina implexa Nyl. and Roccella phycopsis Ach. , were tested. Methyl orsellinate, orcinol, (+)-montagnetol, and for the first time 4-chlororcinol were isolated from Roccella phycopsis. (+)-Usnic acid was obtained from Ramalina implexa. The crude organic extract of both lichen species showed strong antibiotic activity against some bacterial species and nematocidal activity. Among all the pure metabolites tested against the infective juveniles (J2) of the root-knot nematode (RKN) Meloydogine incognita, (+)-usnic acid, orcinol, and (+)-montagnetol had significant nematocidal activity, comparable with that of the commercial nematocide Velum® Prime, and thus they showed potential application in agriculture as a biopesticide. On the contrary, methyl orsellinate and 4-chlororcinol had no nematocidal effect. These results suggest that the substituent pattern at ortho-para-position in respect to both hydroxyl groups of resorcine moiety, which is present in all metabolites, seems very important for nematocidal activity. The organic extracts of both lichens were also tested against some Gram-positive and Gram-negative bacteria. Both extracts were active against Gram-positive species. The extract of Ramalina implexa showed, among Gram-negative species, activity against Escherichia coli and Acinetobacter baumannii, while that from Roccella phycopsis was effective towards all test strains, with the exception of Pseudomonas aeruginosa. The antimicrobial activity of (+)-usnic acid, methyl orsellinate, and (+)-montagnetol is already known, so tests were focused on orcinol and 4-chlororcinol. The former showed antibacterial activity against all Gram positive and Gram-negative test strains, with the exception of A. baumannii and K. pneumoniae, while the latter exhibited a potent antibacterial activity against Gram-positive test strains and among Gram-negative strains, was effective against A. baumannii and K. pneumonia. These results suggest, for orcinol and 4-chlororcinol, an interesting antibiotic potential against both Gram-positive and Gram-negative bacterial strains.
Design and Validation of an Instrument for Technical Performance Indicators of the Kick (Chagi) Technique in Taekwondo

Taekwondo is a martial art and combat sport that originated in medieval or similar cultures, but today it is primarily a combat activity conditioned by safety rules. The kick technique is called “chagi” (Korean language). “Eyeballing” is a problem that is being addressed with traditional training methods used in Taekwondo. (1) Background: To solve this problem, the main aim of this study was to develop an Observation System for Technical Performance Indicators-Chagi (OSTPI-C). The validation and reliability processes were carried out by 19 expert judges, who were required to meet at least four out of the five selected inclusion criteria. (2) Methods: The content validity was calculated using Aiken’s V coefficient value on qualitative (relevance, univocity, and importance) and quantitative levels (rating scale from one to five), through the agreement and consensus of the panel experts. The reliability of the instrument was analyzed using Cronbach’s alpha coefficient. (3) Results: The results demonstrated the high content validity indexes (0.90) and high reliability (alpha > 0.70) of the observational instrument. (4) Conclusions: The OSTIP-C observational instrument could be used in the Olympic and Paralympic Taekwondo sport environment as a valid and reliable tool to evaluate the technical execution of kick technical processes.
Ticagrelor versus Clopidogrel in Patients with Severe Renal Insufficiency Undergoing PCI for Acute Coronary Syndrome

Background Current guidelines recommend the use of potent antiplatelet agents in patients undergoing percutaneous coronary intervention (PCI) following an acute coronary syndrome (ACS). However, data about optimal platelet inhibition in severe renal insufficiency patients are scarce. The purpose of this study is to determine if ticagrelor is more effective than clopidogrel in patients with ACS and severe renal insufficiency treated with PCI. Methods We retrospectively enrolled patients with ACS and severe renal insufficiency (eGFR ≤ 30 ml/min·1.73 m2 or dialysis) who underwent PCI at our hospital between January 2015 and March 2020. We used the adjusted Cox proportional hazards models to analyze the 1-year outcome endpoints, including the primary endpoint (the composite of cardiovascular death, recurrence of MI, or nonfatal ischemic stroke), death from any cause, and bleeding events (Bleeding Academic Research Consortium, BARC criteria). Results A total of 276 patients with ACS and severe renal insufficiency who were treated with PCI with ticagrelor (n = 108) or clopidogrel (n = 168) were included in the study. After adjustment, there was no statistical difference in risk of the primary endpoint (HR, 0.78; 95% CI, 0.46–1.33; P=0.367) and death from any cause (HR, 0.86; 95% CI, 0.38–1.89; P=0.708) in the ticagrelor group against the clopidogrel group. However, the risk of total bleeding was significantly higher in the ticagrelor group (HR, 3.01; 95% CI, 1.81–5.62; P=0.01). Subgroup analysis according to the confounders did not identify any significant subgroup heterogeneity. Conclusion Ticagrelor did not improve the major adverse cardiovascular events and all-cause mortality when compared to clopidogrel, but significantly increased the risk of bleeding in Chinese patients with ACS and severe renal insufficiency undergoing PCI.
A Spatial Adaptation Strategy for Safe Campus Open Spaces during the COVID-19 Pandemic: The Case of Korea University

Open spaces on campus offer various opportunities for students. However, the coronavirus disease (COVID-19) pandemic has affected students’ comfort when occupying open spaces on campus. The purpose of this study is to investigate possible spatial adaptation strategies for safe campus open spaces during the COVID-19 pandemic. For this research, a case study was conducted using a mixed methodology with behavioral mapping that investigated students’ perceptions at Korea University, Seoul, Korea. A qualitative approach was first conducted with behavioral mapping; the results show that despite some behavioral and spatial changes, people still occupy open spaces on campus for various meaningful activities. A quantitative approach with structural equation modeling (SEM) was also conducted to understand the required spatial modifications to improve the safety of open spaces on campus. The positive correlation between (i) social distancing measures, (ii) health protocols, and (iii) accessibility and occupational comfort with (iv) individuals’ fear of COVID-19 as a positive moderation are the four hypotheses proposed in this study. The results suggest that social distancing measures have no correlation with occupational comfort, while accessibility has the largest positive correlation. Suggestions are presented for providing accessible and equally distributed open spaces on campus to avoid overcrowding. Spatial health protocols are also found to positively correlate with occupational comfort, and the perception of the severity of COVID-19 strengthens this correlation. Tangible physical measures to prevent the spread of the virus are necessary to improve students’ sense of comfort and safety in open spaces on campus.
A Narrative Review of Auditory Categorisation and Its Potential Role in Tinnitus Perception

Auditory categorisation is a phenomenon reflecting the non-linear nature of human perceptual spaces which govern sound perception. Categorisation training paradigms may reduce sensitivity toward training stimuli, decreasing the representation of these stimuli in auditory perceptual maps. Reduced cortical representation may have clinical implications for conditions that arise from disturbances in cortical activation, such as tinnitus. This review explores the categorisation of sound, with a particular focus on tinnitus. The potential of categorisation training as a sound-based tinnitus therapy is discussed. A narrative review methodological framework was followed. Four databases (PubMed, Google Scholar, Scopus, and ScienceDirect) were extensively searched for the following key words: categorisation, categorical perception, perceptual magnet effect, generalisation, and categorisation OR categorical perception OR perceptual magnet effect OR generalisation AND sound. Given the exploratory nature of the review and the fact that early works on categorisation are crucial to the understanding and development of auditory categorisation, all study types were selected for the period 1950–2022. Reference lists of articles were reviewed to identify any further relevant studies. The results of the review were catalogued and organised into themes. In total, 112 articles were reviewed in full, from which 59 were found to contain relevant information and were included in the review. Key themes identified included categorical perception of speech stimuli, warping of the auditory perceptual space, categorisation versus discrimination, the presence of categorisation across several modalities, and categorisation as an innate versus learned feature. Although a substantial amount of work focused on evaluating the effects of categorisation training on sound perception, only two studies investigated the effects of categorisation training on tinnitus. Implementation of a categorisation-based perceptual training paradigm could serve as a promising means of tinnitus management by reversing the changes in cortical plasticity that are seen in tinnitus, in turn altering the representation of sound within the auditory cortex itself. In the instance that the categorisation training is successful, this would likely mean a decrease in the level of activity within the auditory cortex (and other associated cortical areas found to be hyperactive in tinnitus) as well as a reduction in tinnitus salience.
N-Propanol Dehydration with Distillation and Pervaporation: Experiments and Modelling

This work is motivated by a fine chemical industry task where n-propanol should be separated from its aqueous mixture. To accomplish this problem, the pervaporation process intends to apply PERVAP™ 1201 type dehydration membranes and to obtain information about the water removal from an aqueous mixture of n-propanol. Different evaluation parameters (selectivities, separation factors, and total fluxes) were experimentally determined. First in the literature, this binary system’s Membrane Flash Index (MFLI) is also determined, confirming the efficiency of pervaporation against flash distillation. The experimental data from pervaporation measurements were evaluated with the improved model by Szilagyi and Toth. It has been established that the model can also be used for this case. The hybrid distillation and pervaporation system is rigorously modelled in a professional flowsheet environment (ChemCAD) and optimized with the dynamic programming optimization method. The distillation-based hybrid method without an extra added extractive agent for separating the n-propanol–water mixture has not yet been published in this computer program. The main objective functions of the hybrid method are the number of minimal theoretical stages and the minimal membrane area. It can be concluded that the process can dehydrate n-propanol with a purity of 99.9 percent.
The Newly Sequenced Genome of Pisum sativum Is Replete with Potential G-Quadruplex-Forming Sequences—Implications for Evolution and Biological Regulation

G-quadruplexes (G4s) have been long considered rare and physiologically unimportant in vitro curiosities, but recent methodological advances have proved their presence and functions in vivo. Moreover, in addition to their functional relevance in bacteria and animals, including humans, their importance has been recently demonstrated in evolutionarily distinct plant species. In this study, we analyzed the genome of Pisum sativum (garden pea, or the so-called green pea), a unique member of the Fabaceae family. Our results showed that this genome contained putative G4 sequences (PQSs). Interestingly, these PQSs were located nonrandomly in the nuclear genome. We also found PQSs in mitochondrial (mt) and chloroplast (cp) DNA, and we experimentally confirmed G4 formation for sequences found in these two organelles. The frequency of PQSs for nuclear DNA was 0.42 PQSs per thousand base pairs (kbp), in the same range as for cpDNA (0.53/kbp), but significantly lower than what was found for mitochondrial DNA (1.58/kbp). In the nuclear genome, PQSs were mainly associated with regulatory regions, including 5′UTRs, and upstream of the rRNA region. In contrast to genomic DNA, PQSs were located around RNA genes in cpDNA and mtDNA. Interestingly, PQSs were also associated with specific transposable elements such as TIR and LTR and around them, pointing to their role in their spreading in nuclear DNA. The nonrandom localization of PQSs uncovered their evolutionary and functional significance in the Pisum sativum genome.
Association between the Human Development Index and Confirmed COVID-19 Cases by Country

It is important to understand the ultimate control of COVID-19 in all countries around the world in relation to the characteristics of developed countries, LDCs, and the variety of transmission characteristics of COVID-19. Therefore, this study aimed to identify factors associated with confirmed cases of COVID-19 with a focus on the Human Development Index (HDI). The units of analysis used for the current study were countries, and dataset were aggregated from multiple sources. This study used COVID-19 data from Our World in Data, the Global Health Security Index, and the WORLD BANK. A total of 171 countries were included in the analysis. A multi-variable linear regression with a hierarchical framework was employed to investigate whether the HDI is associated with confirmed COVID-19 cases after controlling for the demographic and healthcare system characteristics of the study countries. For Model 2, which controlled for demographic and healthcare system characteristics, HDI (β = 0.46, p < 0.001, 95% CI = 2.64–10.87) and the number of physicians per 1000 people (β = 0.34, p < 0.01, 95% CI = 0.21–0.75) had significant associations with the total number of confirmed COVID-19 cases per million people. Countries with a high HDI level are able to conduct higher per capita testing, resulting in higher numbers of confirmed cases than in countries with lower HDI levels. This study has shown evidence that could be used by governments and international organizations to identify national characteristics and provide the international cooperation necessary to develop effective prevention and intervention methods to deal with the global pandemic.
High-Throughput Selection and Experimental Realization of Two New Ce-Based Nitride Perovskites: CeMoN3 and CeWN3

Nitride perovskites have only been experimentally realized in very few cases despite the widespread existence and commercial importance of perovskite materials. From oxide perovskites used in ultrasonics to halide perovskites that have revolutionized the photovoltaics industry, the discovery of new perovskite materials has historically impacted a wide number of fields. Here, we add two new perovskites, CeWN3 and CeMoN3, to the list of experimentally realized perovskite nitrides using high-throughput computational screening and subsequent high-throughput thin film growth techniques. Candidate compositions are first down-selected using a tolerance factor and then thermochemical stability. A novel competing fluorite-family phase is identified for both material systems, which we hypothesize is a transient intermediate phase that crystallizes during the evolution from an amorphous material to a stable perovskite. Different processing routes to overcome the competing fluorite phase and obtain phase-pure nitride perovskites are demonstrated for the CeMoN3–x and CeWN3–x material systems, which provide a starting point for the development of future nitride perovskites. Additionally, we find that these new perovskite phases have interesting low-temperature magnetic behavior: CeMoN3–x orders antiferromagnetically below TN ≈ 8 K with indications of strong magnetic frustration, while CeWN3–x exhibits no long-range order down to T = 2 K but has strong antiferromagnetic correlations. This work demonstrates the importance and effectiveness of using high-throughput techniques, both computational and experimental: they are integral to optimize the process of realizing two entirely novel nitride perovskites.
Helicobacter pylori Thioredoxin1 May Play a Highly Pathogenic Role via the IL6/STAT3 Pathway

Background Recent studies have shown that CagA is considered highly pathogenic to helicobacter pylori (HP) in Western populations. However, in East Asia, CagA positive HP can be up to 90%, but not all patients will lead to gastric cancer. Our research group has found that HP thioredoxin1 (Trx1) may be a marker of high pathogenicity. Here, we investigate whether HP Trx1 exerts high pathogenicity and its internal molecular mechanism. Materials and Methods We constructed the coculture system of high-Trx1 HP and low-Trx1 HP strains with gastric epithelial cell lines separately and detected the influence of HP strains. The cells were stained by AM/PI, and the cell's mortality was assessed by fluorescence microscope. The cell's supernatants or precipitates were collected to detect the expression of IL6. In addition, the cell's precipitates were collected, and the expression of p-STAT3 was detected by western blot. Furthermore, the cell's supernatants were collected for detecting the expression of 8-OHDG to investigate the extent of DNA damage. Results The high-Trx1 HP can cause higher mortality of GES-1 cells compared with the low-Trx1 HP group (high-Trx1 HP (4.53 ± 0.56) %, low-Trx1 HP (0.39 ± 0.10) %, P < 0.001). The mRNA and protein level of IL-6 in AGS and GES-1 cells were increased during HP infection, and the expression of IL-6 in the High-Trx1 HP group was much higher than the low-Trx1 HP group. Besides, the expression of p-STAT3 was higher in the HP-positive gastric mucosa. And the expression of p-STAT3 in the high-Trx1 HP group was significantly upregulated compared with the low-Trx1 HP group. Furthermore, the expression of 8-OHDG in the high-Trx1 group was much higher than the low-Trx1 group (high-Trx1 HP (5.47 ± 1.73) ng/ml, low-Trx1 HP (2.89 ± 1.72) ng/ml, P < 0.05). Conclusion HP Trx1 may play as a marker of high pathogenicity, and the high-Trx1 HP could mediate the pathogenic process of HP infection via the IL6/STAT3 pathway.