Miracle-dz/UniFAR-FTUs huggingface dataset:
Antibacterial Potential of Essential Oils and Silver Nanoparticles against Multidrug-Resistant Staphylococcus pseudintermedius Isolates

Staphylococcus pseudintermedius is an emergent zoonotic agent associated with multidrug resistance (MDR). This work aimed to describe the antibacterial activity of four essential oils (EOs) and silver nanoparticles (AgNPs) against 15 S. pseudintermedius strains isolated from pyoderma. The four EOs, namely Rosmarinus officinalis (RO), Juniperus communis (GI), Citrus sinensis (AR), and Abies alba (AB), and AgNPs were used alone and in combination to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). All strains were MDR and methicillin-resistant. Among the antibiotic cohort, only rifampicin, doxycycline, and amikacin were effective. EOs’ chemical analysis revealed 124 compounds belonging to various chemical classes. Of them, 35 were found in AR, 75 in AB, 77 in GI, and 57 in RO. The monoterpenic fraction prevailed over the sesquiterpenic in all EOs. When EOs were tested alone, AB showed the lowest MIC followed by GI, AR, and RO (with values ranging from 1:128 to 1:2048). MBC increased in the following order: AB, AR, GI, and RO (with values ranging from 1:512 to 1:2048). MIC and MBC values for AgNPs were 10.74 mg/L ± 4.23 and 261.05 mg/L ± 172.74. In conclusion, EOs and AgNPs could limit the use of antibiotics or improve the efficacy of conventional therapies.
Survey of dental diseases in Crioulo breed horses reared in an extensive farming system

ABSTRACT The present study aimed to identify and quantify dental diseases in Crioulo horses reared exclusively under extensive farming conditions. In total, 254 Crioulo horses were evaluated, that were reared exclusively in native pastures with predominantly Eragrostis plana (Annoni grass). The animals were divided into three groups according to age: three to five years (G1), six to 12 years (G2), and 13 to 26 years (G3). In total, 46.1% of the animals in G1, 63.8% in G2, and 82.3% in G3 had two or more incisor disorders; 37.5% in G1, 52.5% in G2 and 45.5% in G3 had two or more disease findings in the canines; and 28.9% in G1, 35.4% in G2, and 64.7% in G3 had four or more disease findings in the second, third, and fourth premolars and molars. However, dental calculus was observed in 90.5% of G2 animals, whereas in G3, the occurrence rate of excessive enamel points and calculus was 82.4%. Although all of the horses studied were reared in an extensive farming system, dental changes were very common in the Crioulo breed reared under these management conditions.
Soil Organic Carbon and Inorganic Carbon Accumulation Along a 30‐year Grassland Restoration Chronosequence in Semi‐arid Regions (China)

Carbon accumulation is an important research topic for grassland restoration. It is requisite to determine the dynamics of the soil carbon pools [soil organic carbon (SOC) and soil inorganic carbon (SIC)] for understanding regional carbon budgets. In this study, we chose a grassland restoration chronosequence (cropland, 0 years; grasslands restored for 5, 15 and 30 years, i.e. RG5, RG15 and RG30, respectively) to compare the SOC and SIC pools in different soil profiles. Our results showed that SOC stock in the 0‐ to 100‐cm soil layer showed an initial decrease in RG5 and then an increase to net C gains in RG15 and RG30. Because of a decrease in the SIC stock, the percentage of SOC stock in the total soil C pool increased across the chronosequence. The SIC stock decreased at a rate of 0·75 Mg hm−2 y−1. The change of SOC was higher in the surface (0–10 cm, 0·40 Mg hm−2 y−1) than in the deeper soil (10–100 cm, 0·33 Mg hm−2 y−1) in RG5. The accumulation of C commenced >5 years after cropland conversion. Although the SIC content decreased, the SIC stock still represented a larger percentage of the soil C pool. Moreover, the soil total carbon showed an increasing trend during grassland restoration. Our results indicated that the soil C sequestration featured an increase in SOC, offsetting the decrease in SIC at the depth of 0–100 cm in the restored grasslands. Therefore, we suggest that both SOC and SIC should be considered during grassland restoration in semi‐arid regions. Copyright © 2016 John Wiley & Sons, Ltd.
Remote Fault Diagnosis for the Powertrain System of Fuel Cell Vehicles Based on Random Forest Optimized with a Genetic Algorithm

To enhance the safety and reliability of fuel cell vehicles, a remote monitoring system based on 5th generation (5G) mobile networks and controller area networks (CANs) was designed, and a random forest (RF) algorithm for the fault diagnosis for eight typical malfunctions of its powertrain system was incorporated. Firstly, the information on the powertrain system was obtained through a 5G-based monitoring terminal, and the Alibaba Cloud IoT platform was utilized for data storage and remote monitoring. Secondly, a fault diagnosis model based on the RF algorithm was constructed for fault classification; its parameters were optimized with a genetic algorithm (GA), and it was applied on the Alibaba Cloud PAI platform. Finally, the performance of the proposed RF fault diagnosis model was evaluated by comparing it with three other classification models: random search conditioning, grid search conditioning, and Bayesian optimization. Results show that the model accuracy, F1 score, and kappa value of the optimized RF fault classification model are higher than the other three. The model achieves an F1 value of 97.77% in identifying multiple typical faults of the powertrain system, as validated by vehicle malfunction data. The method demonstrates the feasibility of remote monitoring and fault diagnosis for the powertrain system of fuel cell vehicles.
Improved Accuracy and Sensitivity in Diagnosis and Staging of Lung Cancer with Systematic and Combined Endobronchial and Endoscopic Ultrasound (EBUS-EUS): Experience from a Tertiary Center

Simple Summary Lung cancer represents the most common form of cancer worldwide and the most frequent cause of cancer-related death in men and women combined. Lung cancer staging is very important, especially in patients who could benefit from surgery. Endobronchial ultrasound (EBUS) and endoscopic ultrasound (EUS) are complementary techniques to explore and acquire tissue from mediastinal lymph nodes by trans-tracheal/bronchial and trans-esophageal approaches, respectively. The respective contribution of separate and combined procedures in the diagnosis and staging of lung cancer has not been fully studied. In our study, a total of 141 patients underwent both procedures, and the combined EBUS-EUS approach in lung cancer patients showed better accuracy and sensitivity in the diagnosis and staging of lung cancer when compared with EBUS and EUS alone. It demonstrated the unmissable aspect of the systematic combination of these endosonographic techniques for an optimal mediastinal diagnosis and staging in lung cancer for patients’ survival. Abstract Background: Combined endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) and endoscopic ultrasound-guided tissue acquisition (EUS-TA) are accurate procedures for the diagnosis and staging of mediastinal lymph nodes (MLNs) in lung cancer. However, the respective contribution of separate and combined procedures in diagnosis and staging has not been fully studied. The aim of this study was to assess their respective performances. Methods: Patients with suspected malignant MLNs in lung cancer or recurrence identified by PET-CT who underwent combined EBUS-TBNA and EUS-TA were retrospectively reviewed. Results: A total of 141 patients underwent both procedures. Correct diagnosis was obtained in 82% with EBUS-TBNA, 91% with EUS-TA, and 94% with the combined procedure. The overall sensitivity, specificity, and positive and negative predictive values (PPV and NPV) of EBUS-TBNA, EUS-TA, and the combined procedure for diagnosing malignancy were [75%, 100%, 100%, 58%], [87%, 100%, 100%, 75%], and [93%, 100%, 100%, 80%], respectively, with a significantly better sensitivity of the combined procedure (p < 0.0001). Staging (82/141 patients) was correctly assessed in 74% with EBUS-TBNA, 68% with EUS-TA, and 85% with the combined procedure. The overall sensitivity, specificity, PPV, and NPV of EBUS-TBNA, EUS-TA, and the combined procedure for lung cancer staging were [62%, 100%, 100%, 55%], [54%, 100%, 100%, 50%], and [79%, 100%, 100%, 68%], respectively, significantly better in terms of sensitivity for the combined procedure (p < 0.001). Conclusion: The combined EBUS-EUS approach in lung cancer patients showed better accuracy and sensitivity in diagnosis and staging when compared with EBUS-TBNA and EUS-TA alone.
Acute Myeloid Leukemia Post Cytotoxic Therapy in Breast Cancer Survivors—Over 23 Years of Single Center Analysis

Background: Acute myeloid leukemia post cytotoxic therapy (AML-pCT) among breast cancer (BC) survivors represents a life-threatening complication. This study aims to assess the clinical outcomes of AML-pCT post BC. Methods: An analysis of all AML patients treated at a single hematology center (2000–2023) was performed to select patients with AML-pCT post BC. We applied the 2022 ELN criteria to define the genetic risk. Results: Among 847 AML patients, 28 were diagnosed with AML-pCT following BC. Complex karyotype (CK) occurred in 23.8% of patients. The median overall survival (OS) was 40 months. The survival outcomes were better after allogenic hematopoietic stem cell transplantation (alloHCT) treatment compared to chemotherapy alone (median OS: 47 versus 7 months, p = 0.008). Patients demonstrating CK showed lower survival compared to those without CK (2-year OS: 25.0% versus 66.2%, p = 0.0048). The multivariable Cox proportional hazards regression model indicated that treatment with alloHCT emerged as a significant factor associated with improved OS. The treatment was associated with superior OS (HR = 0.07, 95% CI = 0.01–0.86, p = 0.04). Conclusions: Patients with AML-pCT following BC were characterized with the highest frequency of adverse genetic risk profiles and demonstrated worse survival rates. AlloHCT should be performed as early as possible in such patients. The growing need for studies on inherited cancer susceptibility underscores the importance of close AML-pCT development monitoring in BC survivors.
Influence of Implant Location, Number, and Design in Ischemic Zone for Implant Prosthesis Success Rate: A Comparative Three-Dimensional Finite Element Analysis

Background: The performance of an implant-supported prosthesis depends on the implant type, number, implant location, and prosthesis design which is directly associated with the distribution of the occlusal forces during mastication. The purpose of the present study is to analyze with three-dimensional (3D) finite element comparative analysis, the influence of implant location, number, and prosthesis design in the mandibular posterior region where multiple posterior teeth replacement is indicated, which in turn is associated with the longevity or Implant success rate. Material and methods: Mandibular posterior section, where 4 teeth are missing, based on the space available for implants and following the surgical guide instructions, a standard make four implants (1st and 2nd premolars {3.8 mm × 11.5 mm}, 1st and 2nd molar {5.1 mm × 11.5 mm}) were selected and with standardization for placement, 4 groups were created with different implant location, number and prosthesis design from the selected implants as model FM1, FM2, FM3, FM4. Finite element analysis was carried out using ANSYS software, version 14.5 (ANSYS Inc., Canonsburg, PA, USA) for assessment of stress, strain, and deformation around implant and bone. Results: Maximum von Mises stress on vertical loading was highest for FM4 (139.55MPa) model (center of prosthesis on premolar and molar pontics) and lowest for FM3 (53.65MPa) model (on 2nd premolar pontic) with values in decreasing order as FM4 ˃ FM2 ˃ FM1 ˃ FM3. Maximum von Mises stress on oblique loading was highest at the distal of 1st molar implant pontic for FM2 (539.81MPa) and lowest at the 2nd premolar pontic for FM3 (352.48MPa) model with values as FM2˃FM1˃FM4˃FM3. Deformation for vertical and oblique loading was observed minimum at the buccal cusp and buccal crestal bone of 2nd premolar, 1st molar on FM3 model against highest deformation on buccal and lingual crestal bone, cuspal area of 2nd premolar, 1st molar implants. For oblique loading minimum deformation was seen for the 2nd premolar, 1st molar cuspal area in FM3, and maximum at the 2nd premolar region in FM1. Conclusion: Four single implants may be chosen if there is enough mesiodistal and buccolingual space to allow for a minimum inter-implant and inter-implant-tooth distance that can be maintained while putting the least amount of stress on the implants and bone. To reduce stress on the bone and implants, it is best to avoid long-span implant-supported prostheses when using fixed implant-supported prostheses.
Advances in Nrf2 Signaling Pathway by Targeted Nanostructured-Based Drug Delivery Systems

Nanotechnology has gained significant interest in various applications, including sensors and therapeutic agents for targeted disease sites. Several pathological consequences, including cancer, Alzheimer’s disease, autoimmune diseases, and many others, are mostly driven by inflammation and Nrf2, and its negative regulator, the E3 ligase adaptor Kelch-like ECH-associated protein 1 (Keap1), plays a crucial role in maintaining redox status, the expression of antioxidant genes, and the inflammatory response. Interestingly, tuning the Nrf2/antioxidant response element (ARE) system can affect immune–metabolic mechanisms. Although many phytochemicals and synthetic drugs exhibited potential therapeutic activities, poor aqueous solubility, low bioavailability, poor tissue penetration, and, consequently, poor specific drug targeting, limit their practical use in clinical applications. Also, the therapeutic use of Nrf2 modulators is hampered in clinical applications by the absence of efficient formulation techniques. Therefore, we should explore the engineering of nanotechnology to modulate the inflammatory response via the Nrf2 signaling pathway. This review will initially examine the role of the Nrf2 signaling pathway in inflammation and oxidative stress-related pathologies. Subsequently, we will also review how custom-designed nanoscale materials encapsulating the Nrf2 activators can interact with biological systems and how this interaction can impact the Nrf2 signaling pathway and its potential outcomes, emphasizing inflammation.
Global research hotspots and trends on robotic surgery in obstetrics and gynecology: a bibliometric analysis based on VOSviewer

Objective Over the last two decades, the quantity of papers published in relation to robotic surgery in obstetrics and gynecology has continued to grow globally. However, no bibliometric analysis based on VOSviewer has been performed to evaluate the past and present of global research in the field. In this study, we aimed to analyze the bibliometric characteristics of papers on robotic surgery in obstetrics and gynecology to reveal research hotspots and trends in this field. Methods The Web of Science Core Collection was searched for scientific papers on robotic surgery in obstetrics and gynecology published between January 1, 1998 and December 31, 2023. Bibliometric metadata of each selected paper was extracted for analysis. The results were visualized by VOSviewer (version 1.6.18). Results A total of 1,430 papers met the inclusion criteria. The United States had the highest total link strengths and contributed the most papers (n = 793). The Mayo Clinic produced the largest number of papers (n = 85), and Professor Pedro T Ramirez contributed the most papers (n = 36). The number of citations ranged from 0 to 295 with a total sum of 29,103. The Journal of Minimally Invasive Gynecology published the most relevant papers (n = 252). Keywords were classified into six clusters based on co-occurrence data, of which cluster 1, cluster 4 and cluster 6 had more main keywords with the largest average publication year. Conclusions This is the first VOSviewer-based bibliometric analysis of robotic surgery research in obstetrics and gynecology. The United States was the leading country, and the Journal of Minimally Invasive Gynecology was the most productive journal in the field. Scientists and institutions from around the world should push their boundaries to bring about deep collaboration. The main research topic has always been the use of robotic surgery in the treatment of gynecologic malignancies. More randomized controlled trials need to be conducted to compare surgical outcomes of robotic surgery with other surgical approaches. Robotic sacrocolpopexy for pelvic organ prolapse has become a new research hotspot, and robotic surgery for sentinel lymph node detection in gynecologic malignancies are more potential directions for future research.
Evaluation of the Anti-Inflammatory and Antioxidant Potential of Cymbopogon citratus Essential Oil in Zebrafish

Simple Summary Lemongrass (Cymbopogon citratus) is frequently consumed as an infusion because of its pharmacological properties, including suspected anti-inflammatory effects. In this study, we examined how lemongrass essential oil, a natural product, can help protect against inflammation and damage caused by stress in cells. We used zebrafish, a small fish often used in scientific research, to determine how different amounts of lemongrass oil affected their health. Our focus was on how the oil influenced the movement of certain immune cells (neutrophils), the healing of the fishes’ tail fin, and the health of their cells. We found that lemongrass oil, at all tested levels, reduced the movement of these immune cells. Interestingly, higher amounts of the oil slowed down the healing of the tail fin. We also noticed changes in the activities of some of the fishes’ protective enzymes, which are important for defending their cells against damage. Our study suggests that lemongrass oil could be useful for its anti-inflammatory properties and might help protect cells from certain types of stress. These findings are valuable because they can lead to a better understanding of natural remedies and their potential use in health and disease management. Abstract This study explored the protective capacity of the essential oil (EO) of Cymbopogon citratus against oxidative stress induced by hydrogen peroxide (H2O2) and the inflammatory potential in zebrafish. Using five concentrations of EO (0.39, 0.78, 1.56, 3.12, and 6.25 μg/mL) in the presence of 7.5 mM H2O2, we analyzed the effects on neutrophil migration, caudal fin regeneration, cellular apoptosis, production of reactive oxygen species (ROS), and activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) after 96 h of exposure. A significant decrease in neutrophil migration was observed in all EO treatments compared to the control. Higher concentrations of EO (3.12 and 6.25 μg/mL) resulted in a significant decrease in caudal fin regeneration compared to the control. SOD activity was reduced at all EO concentrations, CAT activity significantly decreased at 3.12 μg/mL, and GST activity increased at 0.78 μg/mL and 1.56 μg/mL, compared to the control group. No significant changes in ROS production were detected. A reduction in cellular apoptosis was evident at all EO concentrations, suggesting that C. citratus EO exhibits anti-inflammatory properties, influences regenerative processes, and protects against oxidative stress and apoptosis.
The rarity of terrestrial gamma‐ray flashes: 2. RHESSI stacking analysis

We searched for gamma‐ray emission from lightning using the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) satellite by identifying times when RHESSI was near over 2 million lightning discharges localized by the Worldwide Lightning Location Network (WWLLN). We then stacked together the gamma‐ray arrival times relative to the sferic times, correcting for light propagation time to the satellite. The resulting stacked gamma‐ray time profile is sensitive to an average level of gamma‐ray emission per lightning discharge far lower than what can be recognized above background for a single terrestrial gamma‐ray flash (TGF). The summed signal from presumed small, previously unknown TGFs simultaneous with WWLLN discharges is remarkably weak: for the region from 0 to 300 km beneath RHESSI's footprint, (6.2 ± 3.8) × 10−3 detector counts/discharge are measured, as opposed to a typical range of 12–50 detector counts for TGFs identified solely from the gamma‐ray signal. Under the assumption of a broken power law differential distribution of TGF intensities, we find that the index must harden dramatically or cut off just below the sensitivity limit of current satellites and that for most scenarios less than 1% of lightning can produce a TGF that belongs anywhere in the same distribution as those that are observable. For the minority of scenarios where more than a few percent of flashes produce a TGF, most of these “TGFs” are less than 10−4 of the luminosity of the faintest RHESSI TGFs and therefore closer to the luminosity of lightning stepped leaders. The rarity of TGFs holds not only for TGFs simultaneous with the sferic observed by WWLLN but also for any time within 10 ms of the sferic, allowing (for example) for the possibility that different events within the upward propagation of a negative leader in positive intracloud lightning triggered the TGF and WWLLN's detection.
Thermal Conductivity Enhancement of Polymeric Composites Using Hexagonal Boron Nitride: Design Strategies and Challenges

With the integration and miniaturization of chips, there is an increasing demand for improved heat dissipation. However, the low thermal conductivity (TC) of polymers, which are commonly used in chip packaging, has seriously limited the development of chips. To address this limitation, researchers have recently shown considerable interest in incorporating high-TC fillers into polymers to fabricate thermally conductive composites. Hexagonal boron nitride (h-BN) has emerged as a promising filler candidate due to its high-TC and excellent electrical insulation. This review comprehensively outlines the design strategies for using h-BN as a high-TC filler and covers intrinsic TC and morphology effects, functionalization methods, and the construction of three-dimensional (3D) thermal conduction networks. Additionally, it introduces some experimental TC measurement techniques of composites and theoretical computational simulations for composite design. Finally, the review summarizes some effective strategies and possible challenges for the design of h-BN fillers. This review provides researchers in the field of thermally conductive polymeric composites with a comprehensive understanding of thermal conduction and constructive guidance on h-BN design.