Miracle-dz/UniFAR-FTUs huggingface dataset:
Growing in Mixed Stands Increased Leaf Photosynthesis and Physiological Stress Resistance in Moso Bamboo and Mature Chinese Fir Plantations

Mixed-stand plantations are not always as beneficial for timber production and carbon sequestration as monoculture plantations. Systematic analyses of mixed-stand forests as potential ideal plantations must consider the physiological-ecological performance of these plantations. This study aimed to determine whether mixed moso bamboo (Phyllostachys pubescens (Pradelle) Mazel ex J. Houz. ) and Chinese fir (Cunninghamia lanceolata (Lamb.) Hook. ) stands exhibited better physiological-ecological performance than monoculture plantations of these species. We analyzed leaf photosynthesis, chlorophyll fluorescence, antioxidant enzyme activities, chlorophyll content and leaf chemistry in a moso bamboo stand, a Chinese fir stand and a mixed stand with both species. The results showed that both species in the mixed stand exhibited significantly higher leaf net photosynthesis rate (Amax), instantaneous carboxylation efficiency (CUE), chlorophyll content, maximum quantum yield of photosynthesis (Fv/Fm), photochemical quenching coefficient (qP), PSII quantum yield [Y(II)], leaf nitrogen content, and antioxidant enzyme activities than those in the monoculture plantations. However, the non-photochemical quenching (NPQ) in Chinese fir and 2-year-old moso bamboo was significantly lower in the mixed stand than in the monocultures. In addition, the water use efficiency (WUE) of Chinese fir was significantly higher in the mixed stand. The results suggest that the increase in leaf net photosynthetic capacity and the improved growth in the mixed stand could be attributed primarily to the (i) more competitive strategies for soil water use, (ii) stronger antioxidant systems, and (iii) higher leaf total nitrogen and chlorophyll contents in the plants. These findings suggest that mixed growth has beneficial effects on the leaf photosynthesis capacity and physiological resistance of moso bamboo and Chinese fir.
Physiologically Relevant Concentrations of Dolutegravir, Emtricitabine, and Efavirenz Induce Distinct Metabolic Alterations in HeLa Epithelial and BV2 Microglial Cells

Microglia, the resident brain phagocytes, likely play a key role in human immunodeficiency virus (HIV) infection of the central nervous system (CNS) and subsequent neuropathogenesis; however, the nature of the infection-induced changes that yield damaging CNS effects and the stimuli that provoke microglial activation remains elusive, especially in the current era of using antiretroviral (ARV) drugs for ARV therapy (ART). Altered microglial metabolism can modulate cellular functionality and pathogenicity in neurological disease. While HIV infection itself alters brain energy metabolism, the effect of ARV drugs, particularly those currently used in treatment, on metabolism is understudied. Dolutegravir (DTG) and emtricitabine (FTC) combination, together with tenofovir (TAF or TDF), is one of the recommended first line treatments for HIV. Despite the relatively good tolerability and safety profile of FTC, a nucleoside reverse transcriptase inhibitor, and DTG, an integrase inhibitor, adverse side effects have been reported and highlight a need to understand off-target effects of these medications. We hypothesized that similar to previous ART regimen drugs, DTG and FTC side effects involve mitochondrial dysfunction. To increase detection of ARV-induced mitochondrial effects, highly glycolytic HeLa epithelial cells were forced to rely on oxidative phosphorylation by substituting galactose for glucose in the growth media. We assessed ATP levels, resazurin oxidation-reduction (REDOX), and mitochondrial membrane potential following 24-hour exposure (to approximate effects of one dose equivalent) to DTG, FTC, and efavirenz (EFV, a known mitotoxic ARV drug). Further, since microglia support productive HIV infection, act as latent HIV cellular reservoirs, and when dysfunctional likely contribute to HIV-associated neurocognitive disorders, the experiments were repeated using BV2 microglial cells. In HeLa cells, FTC decreased mitochondrial REDOX activity, while DTG, similar to EFV, impaired both mitochondrial ATP generation and REDOX activity. In contrast to HeLa cells, DTG increased cellular ATP generation and mitochondrial REDOX activity in BV2 cells. Bioenergetic analysis revealed that DTG, FTC, and EFV elevated BV2 cell mitochondrial respiration. DTG and FTC exposure induced distinct mitochondrial functional changes in HeLa and BV2 cells. These findings suggest cell type-specific metabolic changes may contribute to the toxic side effects of these ARV drugs.
Moderating Effects of the Ego-Energy in Relation to Stress, Drinking Motives, and Depression in Korean Adult Males

Although there have been numerous studies using stress and coping theories to explain the relationship between stress, drinking motives, and depression, few of them have attempted to verify these theories against adult male data. There is also a shortage of Korean studies, both theoretical and empirical, on the role of ego-energy as a moderating variable in the relationship between stress, drinking motives, and depression. This study uses a multiple-group analysis to investigate the moderating effects of the ego-energy on the aforementioned relationship in adult males. A transactional analysis tool showing the total amount of ego-energy called Ego-gram is used in this study. The tool reflects personality traits based on ego-energy state structural and functional analyses. The researchers collected empirical data to test the research model by conducting an online survey of adult males aged 20–50, residing in seven metropolitan cities and in the Gyeonggi Province. The survey yielded 567 samples. The data were then analyzed through structural equation modeling to understand the relationship between various factors. The results showed that, first, stress was positively correlated with drinking motives. Second, drinking motives had some influence on depression. Third, stress, drinking motives, and depression had statistically significant relationships between low-ego-energy and high-ego-energy groups. Overall, this study found a difference between the two ego-energy groups concerning the relationship between stress, drinking motives, and depression. Based on these results, practical implications were discussed as to how to strengthen the ego-energy, while also presenting future research directions to shed light on the precise mechanism of depression. This study is significant for exploring ways in which adult males cope with and prevent stress and depression, while also offering the basic data for improving the mental and physical health of adult males. This study is also significant for drawing attention to the necessity of developing various health and wellness programs adapted to the needs of this specific population segment while providing the data that may serve as the basis for developing such programs.
Hijacking the Mustard-Oil Bomb: How a Glucosinolate-Sequestering Flea Beetle Copes With Plant Myrosinases

Myrosinase enzymes play a key role in the chemical defense of plants of the order Brassicales. Upon herbivory, myrosinases hydrolyze the β-S-linked glucose moiety of glucosinolates, the characteristic secondary metabolites of brassicaceous plants, which leads to the formation of different toxic hydrolysis products. The specialist flea beetle, Phyllotreta armoraciae, is capable of accumulating high levels of glucosinolates in the body and can thus at least partially avoid plant myrosinase activity. In feeding experiments with the myrosinase-deficient Arabidopsis thaliana tgg1 × tgg2 (tgg) mutant and the corresponding Arabidopsis Col-0 wild type, we investigated the influence of plant myrosinase activity on the metabolic fate of ingested glucosinolates in adult P. armoraciae beetles. Arabidopsis myrosinases hydrolyzed a fraction of ingested glucosinolates and thereby reduced the glucosinolate sequestration rate by up to 50% in adult beetles. These results show that P. armoraciae cannot fully prevent glucosinolate hydrolysis; however, the exposure of adult beetles to glucosinolate hydrolysis products had no impact on the beetle’s energy budget under our experimental conditions. To understand how P. armoraciae can partially prevent glucosinolate hydrolysis, we analyzed the short-term fate of ingested glucosinolates and found them to be rapidly absorbed from the gut. In addition, we determined the fate of ingested Arabidopsis myrosinase enzymes in P. armoraciae. Although we detected Arabidopsis myrosinase protein in the feces, we found only traces of myrosinase activity, suggesting that P. armoraciae can inactivate plant myrosinases in the gut. Based on our findings, we propose that the ability to tolerate plant myrosinase activity and a fast glucosinolate uptake mechanism represent key adaptations of P. armoraciae to their brassicaceous host plants.
Effects of Aerobic Exercise Training on Systemic Biomarkers and Cognition in Late Middle-Aged Adults at Risk for Alzheimer’s Disease

Increasing evidence indicates that physical activity and exercise training may delay or prevent the onset of Alzheimer’s disease (AD). However, systemic biomarkers that can measure exercise effects on brain function and that link to relevant metabolic responses are lacking. To begin to address this issue, we utilized blood samples of 23 asymptomatic late middle-aged adults, with familial and genetic risk for AD (mean age 65 years old, 50% female) who underwent 26 weeks of supervised treadmill training. Systemic biomarkers implicated in learning and memory, including the myokine Cathepsin B (CTSB), brain-derived neurotrophic factor (BDNF), and klotho, as well as metabolomics were evaluated. Here we show that aerobic exercise training increases plasma CTSB and that changes in CTSB, but not BDNF or klotho, correlate with cognitive performance. BDNF levels decreased with exercise training. Klotho levels were unchanged by training, but closely associated with change in VO2peak. Metabolomic analysis revealed increased levels of polyunsaturated free fatty acids (PUFAs), reductions in ceramides, sphingo- and phospholipids, as well as changes in gut microbiome metabolites and redox homeostasis, with exercise. Multiple metabolites (~30%) correlated with changes in BDNF, but not CSTB or klotho. The positive association between CTSB and cognition, and the modulation of lipid metabolites implicated in dementia, support the beneficial effects of exercise training on brain function. Overall, our analyses indicate metabolic regulation of exercise-induced plasma BDNF changes and provide evidence that CTSB is a marker of cognitive changes in late middle-aged adults at risk for dementia.
Safety, Tolerability, and Effect of Nusinersen Treatment in Ambulatory Adults With 5q-SMA

Objective: To determine the safety and tolerability of nusinersen treatment in ambulatory adults with spinal muscular atrophy (SMA) and investigate the treatment effect on muscle strength, physical function, and motor unit physiology. Methods: Individuals aged 18 years or older with genetically confirmed 5q SMA, three or more copies of the SMN2 gene, and the ability to ambulate 30 feet were enrolled. Safety outcomes included the number of adverse events and serious adverse events, clinically significant vital sign or laboratory parameter abnormalities. Outcome assessments occurred at baseline (prior to the first dose of nusinersen) and then 2, 6, 10, and 14 months post-treatment. Results: Six women, seven men (mean age: 37 ± 11, range: 18–59 years) were included for analyses. The most common side effects were headache and back pain, but overall procedures and treatments were well-tolerated. No serious adverse events were reported. Maximal Voluntary Isometric Muscle Contraction Testing (MVICT) and 6-min walk test (6MWT) both showed overall stability with significant increases at 2, 6, and 10 months for the 6MWT. More consistent significant treatment effects were noted on the Hammersmith Functional Motor Scale Expanded, SMA-Functional Rating Scale, and forced vital capacity. Treatment resulted in progressively increased ulnar compound muscle action potential and average single motor unit potential amplitudes, but motor unit number estimation remained stable. Conclusions: Nusinersen treatment is safe and well-tolerated in ambulatory adults with SMA. Treatment resulted in improved motor function and electrophysiological findings suggest that this improvement may be occurring via improved motor unit reinnervation capacity.
Comparison of Immediate and Sequential Withdrawal of a Systemic Glucocorticoid in the Treatment of Acute Exacerbations of Chronic Obstructive Pulmonary Disease: A Multicenter, Randomized, Double-Blind, Parallel-Controlled, Open-Label Study

Patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD) were treated with immediate or sequential withdrawal after 5 days of systemic glucocorticoids. The effects of the two withdrawal methods on the prognosis of patients were compared at 30, 90, 180, and 360 days after discharge. A multicenter, randomized, double-blind, parallel-controlled, open-label study was conducted in the respiratory department of tertiary hospitals in Central China. Patients met inclusion criteria for AECOPD and needed to use systemic glucocorticoids. They were randomly assigned to immediate and sequential withdrawal groups at a 1:1 ratio. The study was completed in August 2020 and is registered at the China Clinical Trials Registry (Chictr.org) (ChiCTR1800018894). According to general data and clinical characteristics, there were no statistically significant differences between the 329 patients in the immediate withdrawal group and the 310 patients in the sequential withdrawal group (P > 0.05). At the 30, 90, 180, and 360-days follow-up, the acute exacerbation frequency, rehospitalization rate, mortality, and intensive care unit (ICU) treatment rate were not significantly different between the immediate withdrawal group and sequential withdrawal group (P > 0.05). The modified Medical Research Council (mMRC) and COPD assessment test (CAT) scores were also not significantly different between the two groups. At the 180- and 360-day follow-up, forced expiratory volume in 1 s (FEV1%) and peak expiratory flow (PEF) were not significantly different between the two groups (P > 0.05). The time from discharge to first acute exacerbation was significantly lower in the immediate withdrawal group (46.12 days) than in sequential withdrawal group (49.02 days) (P < 0.05). The time of stay in the hospital for the first time after discharge was not significantly different between the two groups (P > 0.05). Adverse events were not significantly different between the immediate withdrawal group and sequential withdrawal group (P < 0.05). Subgroup analysis was performed according to age, degree of disease, and relevant indicators. At the 30-day follow-up, the acute exacerbation frequency of patients with advanced age, high global strategy for chronic obstructive lung disease (GOLD), and high fractional exhaled nitric oxide was significantly higher in the immediate withdrawal group than in the sequential withdrawal group (P < 0.05). In addition, according to receiver operating characteristic (ROC) curve analysis, the frequency of acute exacerbations at the 30-day follow-up was significantly higher in patients with age > 63.5 years or GOLD > 3 in the immediate withdrawal group than in the sequential withdrawal group, suggesting that the short-term efficacy was poor.
Evaluating the Impact of Urban Blue Space Accessibility on Housing Price: A Spatial Quantile Regression Approach Applied in Changsha, China

Urban nature spaces are increasingly recognized as essential urban features providing crucial amenities to the residents’ health and well-being. While many studies have been conducted focusing on the influence of green spaces on house prices, very few have explored the impact of urban blue spaces. In this study, we analyzed the proximity effects of different types and sizes of urban blue spaces on property value in Changsha metropolis, China, and examined the spatial quantile effect across different housing prices. A two-stage instrumental method (2SLS) hedonic model was employed to evaluate the impact of different types of urban blue space: river (mainstream and tributary), wetland park, and lakes (large, medium, and small). Spatial quantile regression (SQR) was then used to measure the spatial effect of accessibility on various house price ranges. The 2SLS results show that, except for small-sized lakes, proximity to blue spaces significantly increases property value. Analysis of the SQR model reveals that proximity to major blue spaces increases the marginal willingness-to-pay among homebuyers of high-priced properties, while ordinary blue spaces are more attractive to buyers of low- and medium-priced houses. This may be broadly related to the level of education, utility, and sensitivity to ecosystem services across income groups. Based on these findings, we recommend that urban planners adopt different strategies to promote space utilization efficiency. This is one of the few studies that analyze the capitalization of blue space accessibility in house prices across different quantiles. By categorizing blue spaces and employing the SQR model, this study found the effect of blue spaces on housing prices to be heterogeneous, providing new perspectives to the existing literature.
Coping Strategies and Psychopathological Responses Among Medical and Non-medical Professionals – a Cross-Sectional Online Survey

Background: The SARS-CoV-2 pandemic was announced on March 11th, 2020, due to a surge of newly confirmed cases that significantly impacted populations worldwide, both directly and indirectly. Based on past epidemics research, the mental health implications of introduced restrictions should be expected and adequately addressed irrespective of the practiced profession. Objective: The study aimed to explore psychopathological responses, including post-traumatic stress disorder (PTSD), concerning coping strategy clusters during the COVID-19 pandemic among medical and non-medical workers. Methods: A cross-sectional web survey of the general population of internet users was performed from March 16th to April 26th, 2020, in Poland during the first peak of COVID-19 cases. A sample of 1,831 professionally active respondents, 64.0% of which pursuing a medical career, filled out General Health Questionnaire-28 (GHQ-28), The Impact of Event Scale-Revised (IES-R), and MiniCOPE, along with the socio-demographic questionnaire exploring personal as well as the work-related possibility of direct exposure to contagion and availability of proper protection, contact with the infected without accurate protective measures as well as the adequacy of workers when compared settings. Results: Individuals labeled with specific clusters had significantly different psychopathological manifestations. Irrespective of performed job maladaptive cluster was associated with significantly higher GHQ-28 and IES-R scores on total subscales and all subscales compared to those representing the non-specific and adaptive cluster. Similar findings were observed concerning the frequency of the GHQ-28 positive score. Moreover, the non-specific cluster was associated with significantly higher GHQ-28 total scores among medical professionals. However, GHQ-28 positive scores were significantly more frequent in medical workers using adaptive clusters when compared to non-specific. Such relations were not observed in the non-medical group. IES-R total and subscales' scores did not significantly vary within medical and non-medical groups when adaptive and non-specific clusters were compared. Pursuing a non-medical career was found to be a determinant of lower scores, while female sex was observed to be determinant of higher scores in both GHQ-28 and IES-R scales. Conclusions: Positive screening for psychopathological and PTSD symptoms was expected regardless of the analyzed groups' coping strategies. Given the dramatically developing situation of the COVID-19 pandemic, support initiatives grounded in research evidence may be essential for maintaining the mental well-being and resilience of both the medical and non-medical workforce.
Using Individualized Photobooks to Enhance 3- and 4-Year-Old Children’s Science Identity Through a Science Outreach Program

This paper describes how individualized photobooks were used to support 3- and 4-year-old children in demonstrating their science learning and developing their science identity through participation in a science outreach program. Photographic images stimulate children’s visual thinking and allow them to provide explanations of complex concepts using their language, thus supporting children at their level of understanding. Twenty child/parent dyads were video-recorded interacting with the exhibits during a Science Outreach program into Western Australian community playgroups. Screen shots from the video-recordings were used to develop individual printed photobooks for each child. One week after the program, the photobooks were used in a photo-elicitation conversation with the children (accompanied by their parents) about how the exhibits worked. Children took their photobooks home and 7 weeks after the program parents were interviewed about how the photobooks were used. The photobooks were found to assist the children in demonstrating their science understandings by providing a context for conversation and allowing the children to show their competence, use multiple forms of communication (verbal, non-verbal and through parent), and participate or withdraw on their terms. At home, the photobooks were found to be a focus for the children to share their knowledge of the Outreach program with family members, give the children a voice, and provide them with time to express their understandings. Having the child as narrator of his/her story and the adult as listener empowered the child’s sense of identity. The use of individualized photobooks was found to contribute to the development of the children’s identity and increase their agency in science and enhanced the parents’ perceptions of their children as young scientists.
Regulatory Focus, Motivation, and Their Relationship With Creativity Among Adolescents

Due to the close relationship among intrinsic/extrinsic motivation, regulatory focus, and creativity revealed by previous literature, intrinsic/extrinsic motivation may play a mediating role between regulatory focus and creativity. Therefore, the present study aimed to investigate the relationship between regulatory focus and creativity by combining intrinsic/extrinsic motivation. In this study, senior high school students (n = 418) completed the Regulatory Focus Questionnaire, the Working Preference Inventory, the Williams Creativity Assessment Packet, and the Kirton Adaption–Innovation Inventory. The correlation analysis showed that both promotion and prevention focus positively correlated with intrinsic motivation; intrinsic motivation and promotion focus positively correlated with creativity personality and innovative-adaptive cognitive style; and extrinsic motivation and prevention focus negatively correlated with innovative–adaptive cognitive style. Furthermore, a path model showed that promotion focus positively predicted creativity through the mediation of intrinsic motivation. In general, our study suggests that intrinsic motivation plays a mediating role between promotion focus and creativity. Our results complement those of previous studies and serve as inspiration for the cultivation of creativity in classroom or enterprise settings.