Miracle-dz/UniFAR-FTUs huggingface dataset:
TIMING OF PHOTINUS COLLUSTRANS REPRODUCTIVE ACTIVITY: FINDING A MATE IN TIME (COLEOPTERA: LAMPYRIDAE)

Adult Photinus collustrans fireflies are found from April to October with population peaks in early May and in August. Peaks of male and female abundance occur during the same weeks of the year. Males start nightly signaling first in darker areas of the habitat, and they start earlier when males are more abundant. On the first night females leave their burrows in the soil for mate attraction; most exits correspond with the nightly peak in abundance of signaling males. On subsequent nights, females that remain unmated exit earlier, thus overlapping their availability with more of the male signaling period. Unmated females may remain outside of the burrow long after males have ceased signaling for the night. That females thus continue to expose themselves to the risk of predation with no apparent chance of mating is unexpected and deserves further study. Absolute failure to reproduce can be avoided, in most sexual insects, only if a male and a female find each other in time and space and mate. ' This paper is concerned with the temporal locating of mates by Photinus collustrans LeConte firefly adults. Two components of finding a mate in time are discussed. First, males and females must synchronize their adulthood, so that reproductive maturity occurs simultaneously in the two sexes (seasonal synchrony). Second, males and females must be sexually active at the same time of day (diel synchrony). Photinus collustrans fireflies are found in grassland habitats in the southeastern United States. Soon after sunset males fly and search for females. The females, which are brachypterous and live in burrows in the soil, take positions on the soil surface or on vegetation. Females flash in response to the signals of flying males. Pair formation results from the flash dialogues (Lloyd 1966).
Electrospun Fibres with Hyaluronic Acid-Chitosan Nanoparticles Produced by a Portable Device

Electrospinning is a versatile technique to produce nano/microscale fibrous scaffolds for tissue engineering and drug delivery applications. This research aims to demonstrate that hyaluronic acid-chitosan (HA-CS) nanoparticles can be electrospun together with polycaprolactone (PCL) and gelatine (Ge) fibres using a portable device to create scaffolds for tissue repair. A range of polymer solutions of PCL-gelatine at different weight/volume concentrations and ratios were electrospun and characterised. Fibre–cell interaction (F11 cells) was evaluated based on cell viability and proliferation and, from here, a few polymer blends were electrospun into random or aligned fibre arrangements. HA-CS nanoparticles were synthesised, characterised, and used to functionalise electrospun fibres (8% w/v at 70 PCL:30 Ge), which were chosen based on cell viability. Different concentrations of HA-CS nanoparticles were tested to determine cytotoxicity. A single dosage (1 × 10−2 mg/mL) was associated with higher cell proliferation compared with the cell-only control. This nanoparticle concentration was embedded into the electrospun fibres as either surface modification or blend. Fibres with blended NPs delivered a higher cell viability than unmodified fibres, while NP-coated fibres resulted in a higher cell proliferation (72 h) than the NP-blended ones. These biocompatible scaffolds allow cell attachment, maintain fibre arrangement, promote directional growth and yield higher cell viability.
BRAF V600E and lymph node metastases in papillary thyroid cancer

Objective To evaluate the relationship between the BRAF V600E mutation in lymph node metastasis (LNM) and its invasive characteristics in papillary thyroid cancer (PTC). Material and methods A total of 373 PTC patients were enrolled in this study conducted at Zhujiang Hospital of Southern Medical University between January 2017 and December 2018. PTCs with cervical lymph node metastases were verified pathohistologically, and primary tumors and LNM were examined for the BRAF V600E mutation. Patients were excluded from the study if the BRAF V600E mutation was examined only in primary tumors or only in LNM. Results Of the 373 patients examined, BRAF V600E mutation frequency in primary tumors was slightly higher than in LNM (81.5% vs 78.0%, P = 0.000), the intra-class correlation coefficient (ICC) was 0.865 (95% CI 0.835–0.890). The BRAF V600E mutation in both primary tumor and LNM negatively correlated with the size of the largest metastatic focus of LNM (Odds ratio, OR = 0.297, 95% CI 0.143–0.616, P = 0.001; OR = 0.242, 95% CI 0.119–0.492, P = 0.000, respectively). There was no relationship between BRAF V600E mutation in LNM and the number, extranodal extension or stage of LNM (P > 0.05). Conclusion The BRAF V600E mutation in LNM may not be related to the invasive characteristics of LNM in PTC.
Application and implication of scanning electron microscopy for evaluation of palyno‐morphological features of Vitaceae from Pakistan

The present study aimed to investigate the palyno‐morphological features of species of family Vitaceae from Pakistan. A total of nine species, belonging to four genera were collected, pressed, identified, and then analyzed microscopically. Both quantitative and qualitative characters of the pollen grains were recorded including polar and equatorial diameter, P/E ratio, number of colpi and pores, exine thickness and shapes of the pollen in both polar and equatorial view, and exine sculpturing using Leica microscope fitted with camera Meiji Infinity 1 and then analyzed statistically using software IBM SPSS Statistics 20. The results of the present study demonstrated the variations in polar and equatorial diameter, exine thickness, P/E ratio, pollen shape, and exine sculpturing of the studied species and highlighted the significance of pollen morphology as an identification tool. The present study may contribute to better understand the classification at genus level, which will support the future phylogenetic characterization of the family.
Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

Simple Summary Probiotics are live bacteria, fungi, or yeasts that supplement the gastrointestinal flora and help to maintain a healthy digestive system, thereby promoting the growth performance and overall health of poultry. Probiotics are increasingly being included in poultry diets as an alternative to antibiotics. This systematic review provides a summary of the use of probiotics in poultry production and the potential role of probiotics in the nutrient utilization, growth and laying performance, and gut health of poultry. Abstract Probiotics are live microorganisms which, when administered in adequate amounts, confer health benefits to the host. The use of probiotics in poultry has increased steadily over the years due to higher demand for antibiotic-free poultry. The objective of this systematic review is to present and evaluate the effects of probiotics on the nutrient utilization, growth and laying performance, gut histomorphology, immunity, and gut microbiota of poultry. An electronic search was conducted using relevant keywords to include papers pertinent to the topic. Seventeen commonly used probiotic species were critically assessed for their roles in the performance and gut health of poultry under existing commercial production conditions. The results showed that probiotic supplementation could have the following effects: (1) modification of the intestinal microbiota, (2) stimulation of the immune system, (3) reduction in inflammatory reactions, (4) prevention of pathogen colonization, (5) enhancement of growth performance, (6) alteration of the ileal digestibility and total tract apparent digestibility coefficient, and (7) decrease in ammonia and urea excretion. Thus, probiotics can serve as a potential alternative to antibiotic growth promoters in poultry production. However, factors such as the intestinal health condition of birds, the probiotic inclusion level; and the incubation conditions, feedstuff, and water quality offered to birds may affect the outcome. This systematic review provides a summary of the use of probiotics in poultry production, as well as the potential role of probiotics in the nutrient utilization, growth and laying performance, and gut health of poultry.
Mycosynthesis of silver nanoparticles using extract of endophytic fungi, Penicillium species of Glycosmis mauritiana, and its antioxidant, antimicrobial, anti-inflammatory and tyrokinase inhibitory activity

Silver nanoparticles were synthesized using endophytic fungal species, Penicillium species from Glycosmis mautitiana. Phytochemicals, namely tannins, saponins, terpenoids and flavonoids, were identified in Penicillium species extracts, and act as agents of reducing and capping in the conversion of silver nanoparticles into nanoparticles. Using SEM, UV-spectroscopy and XRD, the Penicillium species silver nanoparticles (PsAgNPs) were characterized. The PsAgNPs are shown to be strong antioxidants (DDPH and FRAP), have demonstrated anti-inflammatory properties by three different methods in vitro and strongly inhibited the activity of xanthine oxidase, lipoxygenase and tyrosine kinase. E. coli and P. aeruginosa bacterial species were strongly inhibited by PsAgNPs activity at maximum levels and SEM picture of P. aeruginosa confirms these effects and that they were shrunken due to the toxic effect of PsAgNPs.
Identification and Analysis of NBS-LRR Genes in Actinidia chinensis Genome

Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the most important disease resistance genes in plants. The genome sequence of kiwifruit (Actinidia chinensis) provides resources for the characterization of NBS-LRR genes and identification of new R-genes in kiwifruit. In the present study, we identified 100 NBS-LRR genes in the kiwifruit genome and they were grouped into six distinct classes based on their domain architecture. Of the 100 genes, 79 are truncated non-regular NBS-LRR genes. Except for 37 NBS-LRR genes with no location information, the remaining 63 genes are distributed unevenly across 18 kiwifruit chromosomes and 38.01% of them are present in clusters. Seventeen families of cis-acting elements were identified in the promoters of the NBS-LRR genes, including AP2, NAC, ERF and MYB. Pseudomonas syringae pv. actinidiae (pathogen of the kiwifruit bacterial canker) infection induced differential expressions of 16 detected NBS-LRR genes and three of them are involved in plant immunity responses. Our study provides insight of the NBS-LRR genes in kiwifruit and a resource for the identification of new R-genes in the fruit.
Root-shoot interactions and plant life forms

A great variety of plant life forms exists in natural vegetations which show different possible solutions to the same problem of carbon-partitioning, namely maintenance of carbon flows under limited access of water and nutrients. The interactions of wa­ ter and nutrient flows on carbon relations are explained and typical features of root/shoot ratios in perennial plants and woody species are discussed. For a hydrostable annual crop plant it is shown that partitioning is controlled by transpiration rate per leaf area and by the capacity of the root for water uptake. For perennial woody species the significance of plant architecture and morphology is demon­ strated for a situation of competitive growth. The occurrence of evergreen and de­ ciduous foliage of woody species is explained with respect to habitat preference and plant geography.
Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming

This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-τ) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM.
Chia, Quinoa, and Their Coproducts as Potential Antioxidants for the Meat Industry

Chia and quinoa have gained popularity among consumers worldwide due to the wide variety of nutrients but also to the bioactive compounds that they contain. Lately, their processing has generated different coproducts (non-commercial grains, flour, partially deoiled flour, rich-fiber fraction, and oil, among others), which could be reincorporated to the food chain with important technological properties, antioxidant activity included. Both sets of ingredients have been revealed a great technological potential for meat product development and innovation, taking into account that oxidation is one of the main reactions responsible for their deterioration and shelf life reduction. This review focuses on the antioxidant compounds of chia and quinoa coproducts and on the strategies used to add them to meat products highlighting their effect on the lipid oxidation control. Apart from the different ways in which quinoa and chia can be incorporated into meat products and their antioxidant properties, innovative approaches for increasing this antioxidant effect and counteracting any negative alterations they may cause will be discussed.
Socio-Demographic Characteristics and Body Weight Perceptions of Study Participants Benefitting Most from the Feel4Diabetes Program Based on Their Anthropometric and Glycaemic Profile Changes

The Feel4Diabetes program was comprised of a community-based screening and a two-year intervention phase aiming to prevent type 2 diabetes (T2D) in families at risk for diabetes across Europe. The current work aimed to identify the socio-demographic characteristics and body weight perceptions of participants who benefitted the most, achieving at least a 5% reduction in body weight, waist circumference and glycaemic indices (fasting plasma glucose, insulin, glycosylated haemoglobin levels), over two-year period. Following a two-stage screening procedure, 2294 high-risk parents were randomly allocated to standard care or more intensive intervention. The participants who benefitted most were living in Southern (OR 2.39–3.67, p < 0.001) and Eastern Europe (OR 1.55–2.47, p < 0.05), received more intensive intervention (OR 1.53–1.90, p = 0.002) and were younger (<40 years old) adults (OR 1.48–1.51, p < 0.05). Furthermore, individuals with tertiary education (OR 2.06, p < 0.001), who were unemployed (OR 1.62–1.68, p < 0.05) and perceived their body weight to be higher than normal (OR 1.58–3.00, p < 0.05) were more likely to benefit from the program. Lastly, males were more likely to show improvements in their glycaemic profiles compared to females (OR 1.40, p = 0.024). These findings point out the regions in Europe and the sociodemographic profile of individuals that benefitted the most in the current study, highlighting the need to prioritise regions in greater need for such interventions and also tailor future interventions to the characteristics and perceptions of the target populations.
Developments toward the Implementation of 44Sc Production at a Medical Cyclotron

44Sc has favorable properties for cancer diagnosis using Positron Emission Tomography (PET) making it a promising candidate for application in nuclear medicine. The implementation of its production with existing compact medical cyclotrons would mean the next essential milestone in the development of this radionuclide. While the production and application of 44Sc has been comprehensively investigated, the development of specific targetry and irradiation methods is of paramount importance. As a result, the target was optimized for the 44Ca(p,n)44Sc nuclear reaction using CaO instead of CaCO3, ensuring decrease in target radioactive degassing during irradiation and increased radionuclidic yield. Irradiations were performed at the research cyclotron at the Paul Scherrer Institute (~11 MeV, 50 µA, 90 min) and the medical cyclotron at the University of Bern (~13 MeV, 10 µA, 240 min), with yields varying from 200 MBq to 16 GBq. The development of targetry, chemical separation as well as the practical issues and implications of irradiations, are analyzed and discussed. As a proof-of-concept study, the 44Sc produced at the medical cyclotron was used for a preclinical study using a previously developed albumin-binding prostate-specific membrane antigen (PSMA) ligand. This work demonstrates the feasibility to produce 44Sc with high yields and radionuclidic purity using a medical cyclotron, equipped with a commercial solid target station.
Faecal characteristics and milk production of dairy cows in early-lactation fed diets differing in forage types in commercial herds

ABSTRACT Effects of grass–clover silage (GCS) alone or combined with whole-crop cereal silage, maize silage (MS) or ensiled pressed sugar beet pulp (EPSB) on faecal characteristics and performance of early-lactation dairy cows were studied in 26 herds. Data were analysed with herd as the experimental unit and correlation coefficients among the variables were estimated. Feeding MS combined with GCS resulted in faeces of a firmer consistency (P < .05), containing more undigested fibre compared to feeding GCS alone or with EPSB (P < .05). There was a strong positive correlation between the number of grains and starch concentration in faeces (r = 0.83, P < .001). Increasing forage dry matter (DM) intake decreased faecal DM concentration (r = −0.54, P < .01) but seems to result in cleaner cows, whereas increasing DM intake of concentrate increased faecal DM concentration (r = 0.63, P < .01) but might result in dirtier cows.
Comparative Peptidomic and Metatranscriptomic Analyses Reveal Improved Gamma-Amino Butyric Acid Production Machinery in Levilactobacillus brevis Strain NPS-QW 145 Cocultured with Streptococcus thermophilus Strain ASCC1275 during Milk Fermentation

The high-gamma-amino butyric acid (GABA)-producing bacterium Levilactobacillus brevis strain NPS-QW 145, along with Streptococcus thermophilus (one of the two starter bacteria used to make yogurt for its proteolytic activity), enhances GABA production in milk. However, a mechanistic understanding of how Levilactobacillus brevis cooperates with S. thermophilus to stimulate GABA production has been lacking. Comparative peptidomic and metatranscriptomic analyses were carried out to unravel the casein and lactose utilization patterns during milk fermentation with the coculture. ABSTRACT The high-gamma-amino butyric acid (GABA)-producing bacterium Levilactobacillus brevis strain NPS-QW 145, along with Streptococcus thermophilus (one of the two starter bacteria used to make yogurt for its proteolytic activity), enhances GABA production in milk. However, a mechanistic understanding of how Levilactobacillus brevis cooperates with S. thermophilus to stimulate GABA production has been lacking. Comparative peptidomic and metatranscriptomic analyses were carried out to unravel the casein and lactose utilization patterns during milk fermentation with the coculture. We found that particular peptides hydrolyzed by S. thermophilus ASCC1275 were transported and biodegraded with peptidase in Lb. brevis 145 to meet the growth needs of the latter. In addition, amino acid synthesis and metabolism in Lb. brevis 145 were activated to further support its growth. Glucose, as a result of lactose hydrolysis by S. thermophilus 1275, but not available lactose in milk, was metabolized as the main carbon source by Lb. brevis 145 for ATP production. In the stationary phase, under acidic conditions due to the accumulation of lactic acid produced by S. thermophilus 1275, the expression of genes involved in pyridoxal phosphate (coenzyme of glutamic acid decarboxylase) metabolism and glutamic acid decarboxylase (Gad) in Lb. brevis 145 was induced for GABA production. SIGNIFICANCE A huge market for GABA-rich milk as a dietary therapy for the management of hypertension is anticipated. The novelty of this work lies in applying peptide profiles supported by metatranscriptomics to elucidate (i) the pattern of casein hydrolysis by S. thermophilus 1275, (ii) the supply of peptides and glucose by S. thermophilus 1275 to Lb. brevis 145, (iii) the transportation of peptides in Lb. brevis and the degradation of peptides by this organism, which was reported to be nonproteolytic, and (iv) GABA production by Lb. brevis 145 under acidic conditions. Based on the widely reported contribution of lactic acid bacteria (LAB) and GABA to human health, the elucidation of interactions between the two groups of bacterial communities in the production of GABA-rich milk is important for promoting the development of functional dairy food and may provide new insight into the development of industrial GABA production.
Failure of Aëdes Aegypti to Transmit Yellow Fever Cultured Virus (17D)1

Summary and Conclusions 1. The quantity of virus in the circulation of a vaccinated human being is too small under most circumstances to permit the infection of Aedes aegypti. 2. Of eight human volunteers fed on by Aedes aegypti on the 6th and 7th days after vaccination, two had minimal amounts of virus on the 7th day. The mosquitoes feeding at that time were not infected. 3. Of twelve lots of mosquitoes fed on rhesus monkeys after vaccination, five lots engorged at a time when the concentration of virus in the blood stream was as great as or greater than that observed in a carefully studied group of twenty-nine human beings after vaccination. None of the five lots was infected. 4. Four attempts were made to infect Aedes aegypti in the larval stage by immersing them in large quantities of vaccine virus. The adults emerging from larvae infected in the two more concentrated virus preparations were shown, by injection into mice and monkeys, to be infected. However, neither lot was capable of transmitting the virus by bite. In one case thirty-five insects failed to transmit by bite, though the identical mosquitoes were shown to contain the virus in their bodies. In the other case twenty-nine insects gave a similar result. None of the three monkeys infected by the injection of the mosquitoes had fever, nor was there any increase in circulating virus as a result of the single passage through the bodies of the mosquitoes. 5. It is concluded that the danger of Aedes aegypti transmitting the 17D vaccine virus is at best slight, and, in all probability, nonexistent.