Miracle-dz/UniFAR-FTUs huggingface dataset:
METABOLOMIC PROFILING OF BOS TAURUS BEEF, DAIRY AND CROSSBRED CATTLE: A BETWEEN-BREEDS META-ANALYSIS.

Cattle breeds may differ substantially in their metabolism. However, the metabolomes of dairy and beef cattle are not well known. Knowledge of breed-specific metabolic features is essential for biomarker identification and to adopt specific nutritional strategies. The muscle hypertrophy (mh), a beef cattle phenotype present in Asturiana de los Valles (AV) but absent in Asturiana de la Montaña (AM) and Holsteins, may underlie such differences. We compared the plasma metabolomes of Holstein, AV, AM and crossbred cattle recipients selected for meta-analysis within an embryo transfer (ET) programme. Blood samples were collected on Day 0 (oestrus) and Day 7 (prior to ET) (N=234 samples x 2 days). Nuclear magnetic resonance quantified N=36 metabolites in plasma, and more metabolic differences between breeds were found on Day 0 (N=19 regulated metabolites) than on Day 7 (N=5). AV and AM largely differed from Holstein cattle (N=55 and N=35 enriched metabolic pathways, respectively); however, AV and AM differed in N=6 enriched pathways. Metabolic activity was higher in AV than in Holstein cattle, as explained in part by the mh phenotype. The metabolomic characterization of breeds facilitates biomarker research and helps to define the healthy ranges of metabolite concentrations.
The effectiveness of supervisor support in lessening perceived uncertainties and emotional exhaustion of university employees during the COVID-19 crisis: the constraining role of organizational intransigence

Abstract Despite the severity of the COVID-19 crisis, which has affected organizations worldwide, there is a lack of research on the organizational factors that affect the psychological wellbeing of the employees of an organization affected by the crisis. This research uses the case of employees at two international universities in Thailand that have been directly affected by the COVID-19 crisis. Grounded in social support theory and the job-demand resource model of job stress, this research examines the role of supervisor support in explaining the degree of perceived uncertainties and emotional exhaustion that employees experience due to the COVID-19 crisis. Moreover, this research examines whether the effect of supervisor support on the perceived uncertainties of employees can be moderated by organizational intransigence, that is, a prevailing climate of resistance to change at the workplace. The questionnaire survey data were obtained from a sample of 300 employees at two private international universities, and the partial least squares structural equation model was used for data analysis. The results significantly confirm that supervisor support has a negative effect on the perceived uncertainties of employees. Perceived uncertainties also significantly mediate the negative effect of supervisor support on the employees’ emotional exhaustion. More importantly, the moderating effect analysis shows that the negative effect of supervisor support on the perceived uncertainties of employees presents only for employees who work in a workplace climate where there is low intransigence; in a workplace climate where there is high intransigence, supervisor support does not lower the perceived uncertainties of employees.
Persistence of Metarhizium flavoviride and consequences for biological control of grasshoppers and locusts.

The residual infectivity of an oil formulation of the fungal entomopathogen Metarhizium flavoviride was measured during a field trial against the rice grasshopper, Hieroglyphus daganensis, in north Benin. The pattern of infectivity was shown to decline exponentially following application, with a half-life of 6.8 days. In this environment, infections due to residual spores from the spray were identified as a key route of infection and accounted for 40-50% of the total infection measured 12 days after application. To examine the within- and between-season consequences of such residual infection, a simple host-pathogen model was developed. The model revealed that even very small increases in residual activity could provide large increases in total mortality and that under certain conditions, residual infection was essential for effective pest control. This aspect of the activity of mycopesticides is rarely considered. The implications of these results are discussed in the context of developing optimum spray strategies for locust and grasshopper control under different ecological conditions.
Biological activity of the n-butanolic extract of Stachys pilifera

2 = 0.871, p < 0.0001). The results of antitumor activity were showed that the extract exhibit antitumor properties. It was showed enhanced inhibitory activity compared to control compounds. It has also been proposed that concentration plays a vital role in increasing the degree of inhabitation.
A Study on the Odonate Larvae of Turkish Thrace: with Larval Identification Keys to the Considered Taxa

The larvae of 27 spp. /sspp. were listed based on the larval specimens of Odonates collected from Turkish Thrace. New locations for the Odonate species known from Turkish Thrace were added to their distributional ranges. Brachytron pratense (Muller) which was reported from only a few localities in the west of Turkey, was also found at a new locality in Turkish Thrace. Furthermore, key for the reported larval species was arranged for the fist time on the larvae of Turkish Odonates.
Endocervical miRNA Expression Profiles in Women Positive for Chlamydia trachomatis with Clinical Signs and/or Symptoms Are Distinct from Those in Women Positive for Chlamydia trachomatis without Signs and Symptoms

Chlamydia trachomatis is the leading cause of sexually transmitted infections that may progress to pelvic inflammatory disease and infertility. No effective vaccine exists for Chlamydia, nor are there biomarkers available that readily predict disease progression. In this cross-sectional pilot study, we recruited symptomatic and asymptomatic women with C. trachomatis (CT) infection and asymptomatic, uninfected control women from an urban sexually transmitted disease clinic to determine if there were differences in microRNA (miRNA) expression. ABSTRACT Chlamydia trachomatis is the leading cause of sexually transmitted infections that may progress to pelvic inflammatory disease and infertility. No effective vaccine exists for Chlamydia, nor are there biomarkers available that readily predict disease progression. In this cross-sectional pilot study, we recruited symptomatic and asymptomatic women with C. trachomatis (CT) infection and asymptomatic, uninfected control women from an urban sexually transmitted disease clinic to determine if there were differences in microRNA (miRNA) expression. Infected women with signs and/or symptoms (CTSS) have distinct miRNA profiles compared to asymptomatic infected women (CTNS). In the CTSS group, miR-142 and -147 showed 2.2- to 6.9-fold increases in expression. In the CTNS group, miR-449c, -6779, -519d, -449a, and -2467 showed 3.9- to 9.0-fold increases in expression. In the CTNS group, cyclins and cell cycle regulation and IL-17 pathways were likely downregulated, while the same signaling pathways were upregulated in the CTSS group. In addition, in the CTSS group, additional inflammatory pathways associated with TNFR1 and IL-8 appear to be upregulated. The miRNA expression patterns differ between CT-infected symptomatic and asymptomatic women, and these differences may warrant further study.
Real-time breath analysis of exhaled compounds upon peppermint oil ingestion by secondary electrospray ionization-high resolution mass spectrometry: technical aspects

Breath analysis by secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) has potential for clinical diagnosis and drug monitoring. However, there is still a lack of benchmarking data that shows the capability of this technique and allows comparability with other breath analysis techniques. In this regard, the goal of this study was the identification of volatile compounds upon ingestion of a specific peppermint oil capsule to get benchmark data for real-time breath analysis with SESI-HRMS. This was done in the framework of a consortium set up by the International Association of Breath Research (IABR), aimed at comparing several analytical instruments for breath analysis. Breath temporal profiles of two subjects were analyzed with SESI-HRMS before and after ingestion of a peppermint oil capsule. The measurements were performed at two different locations using identical SESI-HRMS platforms to allow for comparability and benchmarking. Remarkably, along with the four major compounds (monoterpenes/cineole, menthone, menthofuran and menthol) reported by other members of the consortium, we detected 57 additional features significantly associated (ρ > 0.8) with the peppermint oil capsule, suggesting that this relatively simple intervention might trigger a more complex metabolic cascade than initially expected. This observation was made on both sites. Additional replicate experiments for one of the subjects suggested that a core of 35–40 unique molecules are consistently detected in exhaled breath upon ingestion of the capsule. In addition, we illustrate the analytical capabilities of real-time SESI-HRMS/MS to assist in the identification of unknown compounds. The results outlined herein showcase the performance of SESI-HRMS and enable comparison with other breath analysis techniques. Along with that, they strengthen the potential of this analytical technique for non-invasive drug monitoring and clinical diagnostic purposes.
Safety, Tolerability, and Pharmacokinetics of a Novel Oral Amphotericin B Formulation (iCo-019) following Single-Dose Administration to Healthy Human Subjects: an Alternative Approach to Parenteral Amphotericin B Administration

This study evaluated the safety, tolerability, and pharmacokinetics of a novel oral amphotericin B (AmB) formulation (iCo-019) following single doses to healthy humans. The data from this study suggest that iCo-019 has a long circulation time and systemic exposure without the associated gastrointestinal, liver, and kidney toxicity associated with AmB. This novel oral AmB formulation can serve as a new treatment strategy to overcome the limitations of the use of parenterally administered AmB products. ABSTRACT This study evaluated the safety, tolerability, and pharmacokinetics of a novel oral amphotericin B (AmB) formulation (iCo-019) following single doses to healthy humans. The data from this study suggest that iCo-019 has a long circulation time and systemic exposure without the associated gastrointestinal, liver, and kidney toxicity associated with AmB. This novel oral AmB formulation can serve as a new treatment strategy to overcome the limitations of the use of parenterally administered AmB products.
Parental care and UV coloration in blue tits: opposite correlations in males and females between provisioning rate and mate’s coloration

Parental investment and sexually-selected signals can be intimately related, either because the signals indicate the amount of investment that an individual is prepared to make, and hence its value as a mate (the ‘good parent process’), or because individuals are selected to vary their own investment in relation to their mate’s signals (‘differential allocation’ or ‘reproductive compensation’). Correlations between parental investment and the sexually selected signals of both an individual and its mate are therefore of central interest in sexual selection. Blue tits Cyanistes caeruleus are an ideal study species to investigate such correlations because they provide substantial amounts of biparental care and possess sexually-selected structural UV coloration that seems to signal attractiveness in both sexes. We investigated whether feeding rates of male and female blue tits were correlated with either their own or their mate’s UV coloration, and whether any such correlation was affected by the sex ratio of the brood. We also investigated whether any such correlations were reflected in offspring phenotype. Feeding rates were not correlated with either sex of parent’s own UV coloration. However, they were correlated with the mate’s UV coloration, but in opposite directions in males and females: females had higher feeding rates when mated to bright UV males, implying differential allocation, while males had lower feeding rates when mated to bright UV females, implying reproductive compensation. These relationships were unaffected by the sex ratio of the brood. In addition, fledgling tarsus length, but not mass, was related to male UV coloration, and to female UV coloration in interaction with male age. These results suggest that both male and female attractiveness influence parental investment of the mate, and that this in turn affects offspring phenotype. We found no evidence for differential sex allocation.
Single-center series of boys with recurrent strokes and rotational vertebral arteriopathy

Objective To describe a pediatric stroke syndrome with chronic focal vertebral arteriopathy adjacent to cervical abnormalities. Methods At a single pediatric stroke center, we identified consecutive children with stroke and vertebral arteriopathy of the V3 segment with adjacent cervical bony or soft tissue abnormalities. We abstracted clinical presentation, treatment, and follow-up data from medical charts. Results From 2005 to 2019, 10 children (all boys, ages 6–16 years) presented with posterior circulation strokes and vertebral arteriopathy with adjacent cervical pathology. Two children had bony abnormalities: one had a congenital arcuate foramen and one had os odontoideum with cervical instability. In children without bony pathology, vertebral artery narrowing during contralateral head rotation was visualized by digital subtraction angiography. Eight boys had recurrent ischemic events despite anti-thrombotic treatment (including 5 with multiple recurrences) and were treated surgically to prevent additional stroke. Procedures included vertebral artery decompression (n = 6), endovascular stent and spinal fusion (n = 1), and vertebral artery endovascular occlusion (n = 1). In boys treated with decompression, cervical soft tissue abnormalities (ruptured atlantoaxial bursa, ruptured joint capsule, or connective tissue scarring) were directly visualized during open surgery. No other etiology for stroke or dissection was found in any of the cases. Two boys without recurrent stroke were treated with activity restriction and antithrombotics. At a median follow-up of 51 months (range 17–84), there have been no additional recurrences. Conclusions Children with V3 segmental vertebral arteriopathy frequently have stroke recurrence despite antithrombotics. Cervical bone imaging and angiography with neck rotation can identify underlying pathology.
An induced defense reaction in white spruce to attack by the white pine weevil, Pissodesstrobi

An induced defense reaction in the leaders of white spruce, Piceaglauca (Moench) Voss, occurs in response to attack by the white pine weevil, Pissodesstrobi Peck. The response was initiated shortly after feeding and oviposition in the attacked shoot and consisted of the cambium switching from producing normal tracheids and parenchyma ray cells to producing traumatic resin canals, arranged in a ring fashion in the developing xylem. In sectioned leaders, these traumatic resin canals could be seen emptying their contents into feeding and oviposition cavities dug by the adults, and into the larval galleries. This defense reaction killed eggs and larvae. When the leader survived the attack, the cambium reverted to producing normal xylem tissue, leaving one or more rings of traumatic resin canals embedded in the xylem annual ring.
Risk Factors for grade 2 Disability in Leprosy at the time of Diagnosis

1 Associate Professor, Dept of Physical Medicine and Rehabilitation, Govt. Medical College KozhikodeKerala 2 Assistant Professor, Dept of Dermatology, Govt. Medical College-Kozhikode, KozhikodeKerala 673008 3 Associate Professor, Dept of Community Medicine, Govt. Medical College Kozhikode, KozhikodeKerala 673008 4 Associate Professor, Dept of Dermatology, Govt. Medical College-Kozhikode, KozhikodeKerala 673008 *Corresponding Author Dr Swapna Dominic Email: drswapnadominic@gmail.com, mob: 9447459101 Abstract Background: In the post elimination era of leprosy the major challenge posed by the disease is disability prevention and rehabilitation. Hence studies on disability burden in leprosy should be top priority of research. Objectives: To document grade 2 disability due to leprosy at the time of diagnosis in patients above 12 years attending a tertiary referral centre and to determine the association between age, sex, diagnostic delay, multibacillary disease and leprareaction with grade 2 disability. Methods: Retrospective analysis of case records of leprosy patients who attended a tertiary care centre from 1.1 2017 to 31.12. 2018 was carried out to study the grade 2 disability pattern at diagnosis and its risk factors. Results: During the study period 27/52 leprosy patients (51.9%) who attended our tertiary referral centre had grade 2 disability at diagnosis. Male sex, diagnostic delay, disease manifesting with neurological symptoms as initial event and disease requiring mulibacillary treatment were found to be statistically significant risk factors for grade 2 disability. Conclusions: Our finding of more than 50% cases manifesting grade 2 disability at diagnosis highlights the role rehabilitation specialists in managing the disease. We propose leprosy need a multidisciplinary approach to improve the quality of life of the affected. Retrospective nature of the study and small sanple size were our limitations
EFFICACY OF DIFFERENT HARVESTING TECHNIQUES ON OOCYTE RETRIEVAL FROM BUFFALO OVARIES

A study was undertaken to assess the comparative effi cacy of three harvesting techniques viz., the aspiration, puncture and slicing methods on oocyte recovery in buffalo ovaries obtained from a local abattoir. Effect of ovarian status (luteal phase vs. non-luteal phase), ovarian volume ( 1.5 cm 3 ), side of ovary (right vs. left) and number of visible follicles (No follicles vs.1-5 follicles vs. 6-10 follicles) on oocyte yield and quality were also analyzed. Among the three collection methods, the slicing technique yielded the highest number of total and good quality oocytes, respectively (7.98±0.70 and 3.23±0.30) followed by the puncture (3.46±0.31 and 1.25±0.17) and the aspiration methods (2.38±0.19 and 0.84±0.10). Irrespective of the method employed, a little over 1/3 of recovered oocytes were of poor quality (32.46-34.34%). Luteal phase ovaries (having CL) yielded lower numbers of oocytes compared to non-luteal phase (no CL) ovaries. Both left and right ovaries contributed equally to total as well as different quality grades of oocytes in all the methods. Ovarian volume non-signifi cantly affected the oocyte yield, which was slightly higher in ovaries with a mean volume >1.5 cm 3 . The results indicate that slicing method is superior to the other two methods employed in this study to harvest a greater number of good quality as well as culture grade oocytes from buffalo ovaries.
The alternative complement pathway in ANCA‐associated vasculitis: further evidence and a meta‐analysis

We compared the common pathway components C3a, C5a and membrane attack complex (MAC), also known as C5b‐9, and the alternative pathway components factor B and properdin in patients with ANCA‐associated vasculitis (AAV) and healthy controls, and conducted a meta‐analysis of the available clinical evidence for the role of complement activation in the pathogenesis of AAV. Complement components were evaluated in 59 patients with newly diagnosed or relapsing granulomatosis with polyangiitis or microscopic polyangiitis and 36 healthy volunteers. In 28 patients, testing was repeated in remission. Next, we performed a meta‐analysis by searching databases to identify studies comparing complement levels in AAV patients and controls. A random‐effects model was used for statistical analyses. The median concentrations of MAC, C5a, C3a and factor B were higher in active AAV patients (P < 0·001). Achievement of remission was associated with reductions in C3a (P = 0·005), C5a (P = 0·035) and factor B levels (P = 0·045), whereas MAC and properdin levels did not change. In active AAV, there were no effects of ANCA specificity, disease phenotype, previous immunosuppression or disease severity on complement levels. A total of 1122 articles were screened, and five studies, including this report, were entered into the meta‐analysis. Plasma MAC, C5a and factor B in patients with active AAV were increased compared to patients in remission (excluding factor B) and controls. Changes in C3a were of borderline significance. Our findings and the results of the meta‐analysis support activation of the complement system predominantly via the alternative pathway in AAV patients.
New Material and Phylogenetic Position of Arenysaurus ardevoli, a Lambeosaurine Dinosaur from the Late Maastrichtian of Arén (Northern Spain)

ABSTRACT Arenysaurus ardevoli is a lambeosaurine hadrosaurid from the late Maastrichtian of Arén (Huesca, northern Spain) that has recently been described. The holotype is the first and the most complete lambeosaurine with a braincase from Europe. In this paper, we present a complete description of the postcranial skeleton, which was poorly described when the taxon was named because it was partially unprepared, and new information on several cranial bones (jugal, maxilla, and dentition). A new phylogenetic analysis of Arenysaurus and the closely related Blasisaurus canudoi, also from the late Maastrichtian of Arén, places them inside the Parasaurolophini in a dichotomy with Parasaurolophus spp. Paleobiogeographically, the presence of Arenysaurus and its relationships with other lambeosaurines suggest at least one geodispersal event from Asia to Europe no later than the middle—late Campanian.
Lysine acetylation regulates the RNA binding, subcellular localization and inclusion formation of FUS.

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the preferential death of motor neurons. Approximately 10% of ALS cases are familial and 90% are sporadic. Fused in Sarcoma (FUS) is a ubiquitously expressed RNA binding protein implicated in familial ALS and frontotemporal dementia (FTD). The physiological function and pathological mechanism of FUS are not well understood, particularly whether post-translational modifications play a role in regulating FUS function. In this study, we discovered that FUS was acetylated at lysine-315/316 (K315/K316) and lysine-510 (K510) residues in two distinct domains. Located in the nuclear localization sequence, K510 acetylation disrupted the interaction between FUS and Transportin-1, resulting in the mis-localization of FUS in the cytoplasm and formation of stress granule-like inclusions. Located in the RNA recognition motif, K315/K316 acetylation reduced RNA binding to FUS and decreased the formation of cytoplasmic inclusions. Treatment with deacetylase inhibitors also significantly reduced the inclusion formation in cells expressing ALS mutation P525L. More interestingly, familial ALS patient fibroblasts showed higher levels of FUS K510 acetylation as compared to healthy controls. Lastly, CBP/p300 acetylated FUS, whereas both SIRT and HDAC families of lysine deacetylases contributed to FUS deacetylation. These findings demonstrate that FUS acetylation regulates the RNA binding, subcellular localization, and inclusion formation of FUS, implicating a potential role of acetylation in the pathophysiological process leading to FUS-mediated ALS/FTD.