Miracle-dz/UniFAR-FTUs huggingface dataset:
Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk

The objective of this prospective study was a blinded comparison of three methods for the identification of bacteria isolated on Columbia blood agar from milk samples of dairy cows. Basic biochemical testing, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and 16S rRNA partial genome sequence analysis were compared for bacterial identification to the genus or species level. ABSTRACT The objective of this prospective study was a blinded comparison of three methods for the identification of bacteria isolated on Columbia blood agar from milk samples of dairy cows. Basic biochemical testing, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and 16S rRNA partial genome sequence analysis were compared for bacterial identification to the genus or species level. Milk samples submitted from a commercial dairy farm from recently calved cows or clinical mastitis cases were cultured, and 181 isolates were identified by biochemical testing, MALDI-TOF MS, and 16S rRNA sequence analysis (179 isolates; 2 isolates could not be recovered from storage). For Staphylococcus aureus and Escherichia coli, agreement was determined at the species level. For other microbes, agreement was determined at the genus level or at the group level for streptococcus-like organisms. The positive agreement among all 3 diagnostic methods was 94%, with 95% to 98% between each pair of methods. The overall (including negative agreement) agreement among all 3 methods ranged from 97% to 100%. MALDI-TOF MS is becoming more commonplace for the genus- and/or species-level identification of bacteria isolated from milk samples and, in some laboratories, has replaced conventional biochemical methods. The results of the present study suggest that when identifying pathogens at the genus or group level, conventional culture followed up with either secondary biochemical testing or MALDI-TOF MS is of practical value. For purposes of milk quality and udder health monitoring or research, any of the 3 methods is a valuable tool for genus-level identification of bacteria isolated from dairy cow milk.
Technological Dimension of Pre-School Teacher Training at Tertiary School: Fine Arts Concept-Based Case Study.

The purpose of this study was to experimentally test the impact of the author's Concept and the corresponding educational and methodological support on the technological development of the pedagogical process (based on teaching Fine Arts to pre-schoolers). Quantitative research methods like surveys, interviews, questionnaires, tests, tutor observation check-lists, students’ and learners’ performance records experiment were utilised. Three knowledge field and measurement and evaluation experts were involved to assess the validity of the instruments. The Internal reliability of the questionnaire was validated through running the Kuder-Richardson Formula 20 that resulted in a reliability coefficient, which was 0.72 and indicated internal reliability. The variables to this experiment were the students’ creativity, learning performance, computer skills, motivation of the professional growth and preschool learners’ creative and cognitive thinking and communication skills. It has been found that there was the dynamics in the variables of both student-teachers and children. The mean values for the CG students increased by 0.65 points on average in every variable after the participating in the experiment and the mean figures for children improved by 0.70 points on average as well which proved that the designed model had been efficient in upgrading the pedagogical process at preschools and developing students’ future job-related competencies like creating an interactive technology-mediated and game-based learning environment in Fine Arts which could encourage children’s initiative, creativity curiosity (autonomous cognitive activity) and collaboration along with creation of an emotionally significant situation for autonomous activity of both an individual and a group in which a child's right to their own evaluation, opinion are welcomed. The results met objectives of this study, boosted previous knowledge, and filled the research gaps. After the treatment, there was a 10-12% improvement in learning performance, 7-9% in the EG students’ creativity, 8-10% in computer skills, and 10-11% in motivation of the professional growth and 8-10% in preschool learners’ creative and cognitive thinking and communication skills. The course and methodical complexes for Arts and Design can be recommended for use in the pedagogical process of preschool groups and classes of the Republic of Kazakhstan.
Lider-Üye Etkileşiminin Çalışan Sesliliği ve İşten Ayrılma Niyeti Üzerindeki Etkisi: Psikolojik Güçlendirmenin Düzenleyici Rolü

Gunumuz rekabet ortaminda, calisanlarin isletmeyi, surecleri, urunleri ya da hizmetleri daha ileriye tasiyabilmeleri icin sahip oldugu bilgiyi ve fikirleri ozgurce soyleyebilecegi bir ortam hazirlanmasi gerekmektedir. Calisanlarin psikolojik olarak guclendirilmesi ve lider-uye etkilesiminin saglanmasi bu ortamin yaratilmasinda onemli birer etken olacaktir. Bununla birlikte, isletmelerin en onemli varligi olan calisanlarin isletmeye deger katmasi saglanacak ve rekabet avantajinin ortaya cikmasina yol acacaktir. Bu calismada hizmet sektorunde calisanlarin lider-uye etkilesim duzeylerinin calisan sesliligine ve isten ayrilma niyetine olan etkisini incelemek ve bu iki iliskide psikolojik guclendirmenin moderator etkisini olcmek amaclanmistir. Bu amac dogrultusunda, Kutahya il merkezinde faaliyet gosteren cesitli otel, kafe ve restoran isletmelerinde calisan 155 kisiye ulasilmis ve anket araciligi ile veriler toplanmistir. Elde edilen veriler SPSS 20.0 ve Process Makro eklentisinden yararlanilarak analiz edilmistir. Arastirma sonucuna gore, lider-uye etkilesiminin calisan sesliligine olumlu, isten ayrilma niyetine olumsuz etkisinin oldugunu ve olusan bu etkinin psikolojik guclendirmenin moderator etkisiyle birlikte artti sonucuna ulasilmistir. Diger bir ifadeyle, degiskenler arasinda psikolojik guclendirmenin duzenleyici etkisi arttikca lider-uye etkilesiminin calisan sesliligine olumlu, isten ayrima niyetine uzerine olumsuz etkisinin de arttigi tespit edilmistir.
Pomological Characterization of Main Almond Cultivars from the North Eastern Morocco

ABSTRACT This study concerns pomological characterization of four almond cultivars (Ferragnes, Ferraduel, Marcona and Fournat) introduced in eastern Morocco. The aim is to determine recommended cultivars for this region. Several nut and kernel pomological traits were determined, nut weight ranged between 2.65 g for Ferragnes and 4.41 g for Fournat, and kernel weight ranged between 0.73 g for Marcona and 1.38 g for Fournat. Volume of nuts and kernels ranged, respectively, between 4000.98 mm3 for Ferragnes to 846.09 mm3 for Marcona and 9667.20 mm3–1553.01 mm3 for Fournat. Finally, Ferragnes, Ferraduel and Fournat have kernel yields, reaching 33%, against 20–23% for Marcona which is the most sensitive to drought
Simultaneous detection of native and invasive crayfish and Aphanomyces astaci from environmental DNA samples in a wide range of habitats in Central Europe

Crayfish of North American origin are amongst the most prominent high-impact invasive invertebrates in European freshwaters. They contribute to the decline of European native crayfish species by spreading the pathogen causing crayfish plague, the oomyceteAphanomyces astaci. In this study we validated the specificity of four quantitative PCR (qPCR) assays, either published or newly developed, usable for environmental DNA (eDNA) screening for widely distributed native and non-native crayfish present in Central Europe:Astacus astacus,Pacifastacus leniusculus,Faxonius limosusandProcambarus virginalis. We then conducted an eDNA monitoring survey of these crayfish as well as the crayfish plague pathogen in a wide variety of habitat types representative for Central and Western Europe. The specificity of qPCR assays was validated against an extensive collection of crayfish DNA isolates, containing most crayfish species documented from European waters. The three assays developed in this study were sufficiently species-specific, but the published assay forF. limosusdisplayed a weak cross-reaction with multiple other crayfish species of the family Cambaridae. In the field study, we infrequently detected eDNA ofA. astacitogether with the three non-native crayfish species under examination. We never detected eDNA fromA. astacitogether with native crayfish, but in a few locations eDNA from both native and non-native crayfish was captured, due either to passive transport of eDNA from upstream populations or co-existence in the absence of infected crayfish carriers ofA. astaci. In the study, we evaluated a robust, easy-to-use and low-cost version of the eDNA sampling equipment, based mostly on items readily available in garden stores and hobby markets, for filtering relatively large (~5 l) water samples. It performed just as well as the far more expensive equipment industrially designed for eDNA water sampling, thus opening the possibility of collecting suitable eDNA samples to a wide range of stakeholders. Overall, our study confirms that eDNA-based screening for crayfish and their associated pathogen is a feasible alternative to traditional monitoring.
Physicochemical, microbiological and bioactive evaluations of ‘Araçá-Boi’ (Eugenia stipitata Mc Vaugh) pulp exposed to Gamma Irradiation

The aim of this research was to evaluate the physicochemical, microbiological and bioactive quality of ‘Araca-Boi’ pulps exposed to Gamma Irradiation. Manual harvesting was done in the morning, using good agricultural practices, and fruits were packed in thermal boxes and transported to the ‘Laboratorio de Armazenamento e Processamento de Produtos Agricolas’ of the ‘Universidade Federal de Campina Grande’, to obtain the pulp. The ‘Araca-Boi’ pulp was irradiated in the ‘Laboratorio de Irradiacao Gama do Centro de Desenvolvimento de Tecnologia Nuclear’ (CDTN), located at UFPE - Recife, PE, where three doses of Gamma Irradiation (2, 3, 4 kGy) were applied for later comparison with the non-irradiated sample (control). After irradiation, the microbiological, physicochemical parameters and the quantification of vitamin C and flavonoids were evaluated it can be observed that dose 6 is grouped in a set with differential characteristics of the other treatments, as it provided higher AA, pH, luminosity and water activity (A w ). It was observed that there was no microorganism development after irradiation in all analyzed treatments. It can be concluded that the different doses of Gamma Irradiation guaranteed the microbiological quality of the ‘Araca-Boi’ pulp, remaining fit for consumption and in compliance with the Brazilian legislation.
COVID-19: Response Strategies & Employee Conviction - A prospective of Hotel Chain in NCR region

This study analyzed the relationship between Covid-19 response strategies and employees Faith in chosen Hotels inside Eastern NCR Region. The cross-sectional study configuration was received and information was produced from 144 employees chose utilizing the basic simple random sampling from a populace of 242 workers of five chose Hotels in Eastern NCR Region. The examination, being prevalently nomothetic in the system, observationally dis-covers the unique relationship of two distinguished experimentally referents of the indicator variable, Covid-19 response strategies, in particular; Communication & monitoring with the model variable, representative Faith utilizing the Spearman’s rank order correlation measurable tool. The information assortment instrument comprised of the poll with discoveries uncovering that Covid-19 response strategies, such as, Communication & monitoring essen-tially associate with worker Faith. In light of these discoveries, it was inferred that Effective and transparent communication and monitoring systems and exercises altogether improve representative Faith in associations and from that point we suggested that associations, particularly Hotels, Adopt more transparent and practical approach which translate confidence and compe-tency on their part especially in given crises situation such as COVID 19. The executives ought to likewise try to empower and console staff, clients, and different partners of their pledge to guarantee the security and prosperity of every single invested individual during and significantly after crises as this would Further promote Faith and in this manner empower a progressively helpful work environment.
An Elongational and Shear Evaluation of Polymer Viscoelasticity during Flow in Porous Media

This paper uses a combination of approaches to evaluate the viscoelastic phenomenon in high-molecular-weight polymers (24–28 M Daltons) used for enhanced oil recovery (EOR) applications. Rheological data were cross-analyzed with single- and two-phase polymer flooding experiments in outcrop cores and micromodels, respectively. First, the impact of semi-harsh conditions (salinity, hardness, and temperature) was evaluated. Second, the impact of polymer degradation (sand face flow), focusing on the viscoelastic properties, was investigated. Finally, polymer viscoelastic properties were characterized, proposing a threefold rheological approach of rotational, oscillatory, and elongational behavior. Data from the rheological approaches were cross-analyzed with core flooding experiments and performed at a room temperature of 22 °C and at a higher temperature of 55 °C. The change in polymer viscoelastic properties were analyzed by investigating the effluents from core flooding experiments. Oil recovery experiments in micromodel helped our understanding of whether salinity or hardness has a dominating impact on in situ viscoelastic polymer response. These approaches were used to study the impact of mechanical degradation on polymer viscoelasticity. The brines showed notable loss in polymer viscoelastic properties, specifically with the hard brine and at higher temperature. However, the same polymer solution diluted in deionized water exhibited stronger viscoelastic properties. Multiple flow-behaviors, such as Newtonian, shear thinning, and thickening dominated flow, were confirmed through pressure drop analysis against interstitial velocity as already reported by other peer researchers. Turbulence-dominated excessive pressure drop in porous media was calculated by comparing core flood pressure drop data against pressure data in extensional viscometer–rheometer on a chip (eVROC®). In addition, a significant reduction in elastic-dominated flow was confirmed through the mechanical degradation that happened during core flood experiments, using various approaches. Finally, reservoir harsh conditions (high temperature, hardness, and salinity) resulted in a significant reduction in polymer viscoelastic behavior for all approaches.
Relationship between Arch Base Length and Dental Crowding in Different Skeletal Patterns

This study aimed to assess the maxillary and mandibular effective arch lengths in a Malaysian population sample and to verify the relationship between effective arch length and dental crowding in patients with different skeletal patterns. A sample of 96 subjects was divided into three groups according to skeletal pattern (I,II and III), each group was further divided into 2 sub-groups (based on the amount of maxillary and mandibular arch crowding) sub-group1(≤4mm) and sub-group2 (>4mm). The maxillary and mandibular effective arch lengths were measured on cephalometric radiographs. Descriptive statistics and Analysis of Variance (ANOVA) were used for analysis. The results showed that this Malaysian sample have smaller mean values of effective arch length compared with other populations. Maxillary base length was highest in Class I, followed by Class II and Class III. Mandibular base length was highest in Class III, followed by Class I and Class II. No statistically significant differences were found in maxillary and mandibular base length between the 2 subgroups of crowding in all skeletal patterns. Further analysis for the 2 crowding sub-groups in the maxilla showed no statistically significant differences in base length for the maxilla (P=0.132) and mandible (P=0.215). Similarly, in subjects with mandibular crowding, no statistically significant differences in base length were detected in the maxilla (P=0.359) and mandible (P=0.948). A baseline data for maxillary and mandibular effective arch lengths for Malaysian population was established in this study. Arch base length and crowding need to be further investigated. Clinical article (J Int Dent Med Res 2020; 13(4): 1349-1354)
A Modified ABCD Model with Temperature-Dependent Parameters for Cold Regions: Application to Reconstruct the Changing Runoff in the Headwater Catchment of the Golmud River, China

The runoff changes due to global warming in hydrological basins in the Qinghai–Tibetan Plateau (QTP) have received worldwide attention. The headwater catchment of the Golmud River, located in the northern QTP, is the main source of water resources for the Golmud city in an arid region but has been poorly known for the hydroclimatological behaviors. In this study, a widely-used hydrological model, the ABCD model (Thomas, H.A., Washington, DC, USA), is modified by incorporating temperature-dependent hydrological processes and groundwater evapotranspiration in cold regions with a few additional parameters. The new model is used to reconstruct the monthly runoff in the past decades for the headwater catchment of the Golmud River and performs better than other comparable models. As indicated, the annual snowmelt runoff increased with the increasing air temperature and became more concentrated in April than in May. The frozen soil degradation could increase the hydraulic conductivity of soils and lead to a rise in cold season runoff. The groundwater level in the Golmud city was positively correlated to the annual runoff in the headwater catchment of the Golmud River, indicating that an increase of the groundwater level could be triggered by the rising trend in the streamflow of the Golmud River. This study suggests a useful hydrological model for the groundwater management in the Golmud city.
Correlation of clinical and histopathological diagnoses of oral mucosal lesions at tertiary care centre: a retrospective study

<p class="abstract"><strong>Background:</strong> The objective of the study was to study the correlation between clinical and histopathological diagnoses of oral lesions. </p><p class="abstract"><strong>Methods:</strong> Data of all patients attending the department of Dermatology KEM Hospital, Mumbai with oral mucosal lesions who underwent biopsy for histopathological examination in a duration of one year was included in this retrospective study. Their clinical and histopathological diagnoses were correlated and data was analysed. <strong></strong></p><p class="abstract"><strong>Results:</strong> A data of total of 164 patients was included in study. Out of the clinically diagnosed, histopathological correlation was found to be 66.66% for oral leucoplakia, 81.25% for lichen planus, 72% for squamous cell carcinoma, 88% for pemphigus vulgaris and 75% for submucosal fibrosis. Overall correlation found was 75.60%. </p><p class="abstract"><strong>Conclusions:</strong> Histopathological examination of oral mucosal lesion is very important to arrive at the accurate diagnosis and to plan definitive treatment. Histopathological examination of oral mucosal lesions must be done routinely because wide variety of conditions present with similar morphologic features and can be the initial signs of many skin disorders. </p>
GENDER PERSPEKTIF INTERPRETASI TEKS DAN KONTEKSTUAL

Abstrak Artikel ini dimaksudkan untuk memberikan gambaran bagaimana konstruksi bias gender dalam perspektif tafsir dan realitas yang telah sekian lama melembaga dan legal di tengah-tengah masyarakat, baik yang meliputi metode penafsiran bias gender, penafsiran ayat-ayat bias gender, serta tekstual dan kontekstual. Pembahasan ini tentunya bertujuan sebagai ventilasi dan ruang gerak yang bebas dalam interpretasi ayat-ayat Al-Qur’an sebagai kontekstualitas ajaran-ajaran Islam agar selaras dengan perkembangan zaman bagi para muffasir kontemporer. Penelitian ini menggunakan metode yang bersifat deskriptif kualitatif dalam lingkup kajian pustaka (library research). Hasil penelitian ini menunjukan bahwa konstruksi bias gender dalam perspektif penafsiran dan realita menghasilkan tatanan di mana laki-laki selalu dicitrakan dalam posisi yang unggul hampir dalam sejarah Muslim, sementara perempuan selalu berada dalam citra yang lemah dengan didukung oleh penafsiran ayat-ayat Al-Qur’an dan al-Hadits yang bersifat misoginis secara harfiah, naratif, dan legal.
Transitions in cell potency during early mouse development are driven by Notch

The Notch signalling pathway plays fundamental roles in diverse developmental processes in metazoans, where it is important in driving cell fate and directing differentiation of various cell types. However, we still have limited knowledge about the role of Notch in early preimplantation stages of mammalian development, or how it interacts with other signalling pathways active at these stages such as Hippo. By using genetic and pharmacological tools in vivo, together with image analysis of single embryos and pluripotent cell culture, we have found that Notch is active from the 4-cell stage. Transcriptomic analysis in single morula identified novel Notch targets, such as early naïve pluripotency markers or transcriptional repressors such as TLE4. Our results reveal a previously undescribed role for Notch in driving transitions during the gradual loss of potency that takes place in the early mouse embryo prior to the first lineage decisions.
Long-term impacts of livestock grazing and browsing in the Succulent Karoo: a 20-year study of vegetation change under different grazing regimes in Namaqualand§

This study used a fence-line contrast approach to investigate the long-term impact of high grazing pressure on the vegetation at a site in Namaqualand, South Africa. Forty pairs of permanently marked plots were surveyed in 1996, 2006 and 2016. The main objective was to investigate changes in the vegetation structure and species composition between the near-continuously grazed communal rangelands and the relatively lightly grazed commercial rangelands over the 20-year period. The results showed a decline in total vegetation cover in both commercial and communal rangelands in 2016 relative to the two earlier sampling periods. This can be attributed to the low rainfall in 2016 and was due largely to a reduction in annual plant cover, especially on the communal rangeland. Perennial shrub species provide a fodder bank that can be utilised by livestock in times of drought and can buffer short-term deficits in forage supply. However, the annuals that dominate the vegetation of the communal rangeland do not form such fodder banks and consequently do not have the same multi-year buffering capacity as perennial shrubs. This provides the mechanism whereby long-term continuous grazing decreases resilience to rainfall fluctuations and increases livestock variability, thereby promoting non-equilibrium-type dynamics in the system.