Miracle-dz/UniFAR-FTUs huggingface dataset:
Npas1+-Nkx2.1+ Neurons Are an Integral Part of the Cortico-pallido-cortical Loop

Within the basal ganglia circuit, the external globus pallidus (GPe) is critically involved in motor control. Aside from Foxp2+ neurons and ChAT+ neurons that have been established as unique neuron types, there is little consensus on the classification of GPe neurons. Properties of the remaining neuron types are poorly defined. In this study, we leverage new mouse lines, viral tools, and molecular markers to better define GPe neuron subtypes. We found that Sox6 represents a novel, defining marker for GPe neuron subtypes. Lhx6+ neurons that lack the expression of Sox6 were devoid of both parvalbumin and Npas1. This result confirms previous assertions of the existence of a unique Lhx6+ population. Neurons that arise from the Dbx1+ lineage were similarly abundant in the GPe and displayed a heterogeneous makeup. Importantly, tracing experiments revealed that Npas1+-Nkx2.1+ neurons represent the principal noncholinergic, cortically-projecting neurons. In other words, they form the pallido-cortical arm of the cortico-pallido-cortical loop. Our data further show that pyramidal-tract neurons in the cortex collateralized within the GPe, forming a closed-loop system between the two brain structures. Overall, our findings reconcile some of the discrepancies that arose from differences in techniques or the reliance on preexisting tools. Although spatial distribution and electrophysiological properties of GPe neurons reaffirm the diversification of GPe subtypes, statistical analyses strongly support the notion that these neuron subtypes can be categorized under the two principal neuron classes: PV+ neurons and Npas1+ neurons. SIGNIFICANCE STATEMENT The poor understanding of the neuronal composition in the external globus pallidus (GPe) undermines our ability to interrogate its precise behavioral and disease involvements. In this study, 12 different genetic crosses were used, hundreds of neurons were electrophysiologically characterized, and >100,000 neurons were histologically- and/or anatomically-profiled. Our current study further establishes the segregation of GPe neuron classes and illustrates the complexity of GPe neurons in adult mice. Our results support the idea that Npas1+–Nkx2.1+ neurons are a distinct GPe neuron subclass. By providing a detailed analysis of the organization of the cortico-pallidal-cortical projection, our findings establish the cellular and circuit substrates that can be important for motor function and dysfunction.
Type 1 or Type 2 Myocardial Infarction in Patients with a History of Coronary Artery Disease: Data from the Emergency Department

A type 2 myocardial infarction (T2MI) is the result of an imbalance between oxygen supply and demand, without acute atherothrombosis. T2MI is frequent in emergency departments (ED), but has not been extensively evaluated in patients with previously known coronary artery disease (CAD). Our study assessed the incidence and characteristics of T2MI compared to type 1 (T1MI) in CAD patients admitted to an ED. Among 33,669 consecutive patients admitted to the ED, 2830 patients with T1MI or T2MI were systematically included after prospective adjudication by the attending clinician according to the universal definition. Among them, 619 (22%) patients had a history of CAD. Using multivariable analysis, CAD history was found to be an independent predictive factor of T2MI versus T1MI (odds ratio (95% confidence interval) = 1.38 (1.08–1.77), p = 0.01). Among CAD patients, those with T2MI (n = 254) were older (median age: 82 vs. 72 years, p < 0.001), and had more frequent comorbidities and more frequent three-vessel disease at the coronary angiography (56% vs. 43%, p = 0.015). Percutaneous coronary intervention was by far less frequent after T2MI than after T1MI (28% vs. 67%, p < 0.001), and in-hospital mortality was twice as high in T2MI (15% vs. 7% for T1MI, p < 0.001). Among biomarkers, the C reactive protein (CRP)/troponin Ic ratio predicted T2MI remarkably well (C-statistic (95% confidence interval) = 0.84 (0.81–0.87, p < 0.001). In a large unselected cohort of MI patients in the ED, a quarter of patients had previous CAD, which was associated with a 40% higher risk of T2MI. CRP/troponin ratios could be used to help distinguish T2MI from T1MI.
Casimicrobium huifangae gen. nov., sp. nov., a Ubiquitous “Most-Wanted” Core Bacterial Taxon from Municipal Wastewater Treatment Plants

The activated sludge process is the most widely applied biotechnology and is one of the best ecosystems to address microbial ecological principles. Yet, the cultivation of core bacteria and the exploration of their physiology and ecology are limited. In this study, the core and novel bacterial taxon C. huifangae was cultivated and characterized. This study revealed that C. huifangae functioned as an important module hub in the activated sludge microbiome, and it potentially plays an important role in municipal wastewater treatment plants. ABSTRACT Microorganisms in wastewater treatment plants (WWTPs) play a key role in the removal of pollutants from municipal and industrial wastewaters. A recent study estimated that activated sludge from global municipal WWTPs harbors 1 × 109 to 2 × 109 microbial species, the majority of which have not yet been cultivated, and 28 core taxa were identified as “most-wanted” ones (L. Wu, D. Ning, B. Zhang, Y. Li, et al., Nat Microbiol 4:1183–1195, 2019, https://doi.org/10.1038/s41564-019-0426-5). Cultivation and characterization of the “most-wanted” core bacteria are critical to understand their genetic, physiological, phylogenetic, and ecological traits, as well as to improve the performance of WWTPs. In this study, we isolated a bacterial strain, designated SJ-1, that represents a novel cluster within Betaproteobacteria and corresponds to OTU_16 within the 28 core taxa in the “most-wanted” list. Strain SJ-1 was identified and nominated as Casimicrobium huifangae gen. nov., sp. nov., of a novel family, Casimicrobiaceae. C. huifangae is ubiquitously distributed and is metabolically versatile. In addition to mineralizing various carbon sources (including carbohydrates, aromatic compounds, and short-chain fatty acids), C. huifangae is capable of nitrate reduction and phosphorus accumulation. The population of C. huifangae accounted for more than 1% of the bacterial population of the activated sludge microbiome from the Qinghe WWTP, which showed seasonal dynamic changes. Cooccurrence analysis suggested that C. huifangae was an important module hub in the bacterial network of Qinghe WWTP. IMPORTANCE The activated sludge process is the most widely applied biotechnology and is one of the best ecosystems to address microbial ecological principles. Yet, the cultivation of core bacteria and the exploration of their physiology and ecology are limited. In this study, the core and novel bacterial taxon C. huifangae was cultivated and characterized. This study revealed that C. huifangae functioned as an important module hub in the activated sludge microbiome, and it potentially plays an important role in municipal wastewater treatment plants.
Equilibrium structure and deformation response of 2D kinetoplast sheets

Significance We study the polymer physics of complex DNA networks called kinetoplasts, membrane-like structures consisting of thousands of rings connected together like chainmail, found in the mitochondria of parasites that cause tropical diseases, such as Leishmaniasis. We make observations of individual kinetoplasts in solution to measure their equilibrium properties, finding that kinetoplasts adopt a hemispherical configuration with strong intrinsic curvature, which undergoes small-amplitude fluctuations at subsecond timescales, and can stretch and compress elastically in confinement. We anticipate that our findings will establish kinetoplasts as a model system for single-molecule studies of topologically complex 2-dimensional polymer systems. The considerable interest in two-dimensional (2D) materials and complex molecular topologies calls for a robust experimental system for single-molecule studies. In this work, we study the equilibrium properties and deformation response of a complex DNA structure called a kinetoplast, a 2D network of thousands of linked rings akin to molecular chainmail. Examined in good solvent conditions, kinetoplasts appear as a wrinkled hemispherical sheet. The conformation of each kinetoplast is dictated by its network topology, giving it a unique shape, which undergoes small-amplitude thermal fluctuations at subsecond timescales, with a wide separation between fluctuation and diffusion timescales. They deform elastically when weakly confined and swell to their equilibrium dimensions when the confinement is released. We hope that, in the same way that linear DNA became a canonical model system on the first investigations of its polymer-like behavior, kinetoplasts can serve that role for 2D and catenated polymer systems.
Apple Preload Halved the Postprandial Glycaemic Response of Rice Meal in Healthy Subjects

This study aimed to investigate the possible glycemic effect of apple preload on acute postprandial glycemic responses (GRs) of a following rice meal, comparing with its co-ingestion counterpart and an apple sugar solution preload, based on equal carbohydrates intake. In a randomized crossover trial, 18 healthy female subjects consumed (1) rice, (2) co-ingestion of apple and rice (A+R), (3) apple preload and rice (PA+R), and (4) rice with sugar solution preload (same sugar profile as in apple) (PSS+R). Acute postprandial GR tests and subjective satiety tests were carried out for each test food. Compared with rice reference, the PA+R achieved a 50% reduction of the iAUC0-120, a 51.4% reduction of the average peak value, and a 52.6% reduction of glycemic excursion in 240 min, while the PSS+R showed 29.7% and 31.6% reduction of peak value and glycemic excursion, respectively. No significant differences were found between R and PA+R in any of the satiety characteristics. Compared with rice control, apple preload of 15 g available carbohydrates remarkably lowered the acute postprandial GR without negative effect on satiety. The sugar component may partly contribute to the glycemic suppressing effect of the apple preload.
Hypoxia: Overview on Hypoxia-Mediated Mechanisms with a Focus on the Role of HIF Genes

Hypoxia represents a frequent player in a number of malignancies, contributing to the development of the neoplastic disease. This review will discuss the means by which hypoxia powers the mechanisms behind cancer progression, with a majority of examples from lung cancer, the leading malignancy in terms of incidence and mortality rates (the frequent reference toward lung cancer is also for simplification purposes and follow up of the global mechanism in the context of a disease). The effects induced by low oxygen levels are orchestrated by hypoxia-inducible factors (HIFs) which regulate the expression of numerous genes involved in cancer progression. Hypoxia induces epithelial-to-mesenchymal transition (EMT) and metastasis through a complex machinery, by mediating various pathways such as TGF-β, PI3k/Akt, Wnt, and Jagged/Notch. Concomitantly, hypoxic environment has a vast implication in angiogenesis by stimulating vessel growth through the HIF-1α/VEGF axis. Low levels of oxygen can also promote the process through several other secondary factors, including ANGPT2, FGF, and HGF. Metabolic adaptations caused by hypoxia include the Warburg effect—a metabolic switch to glycolysis—and GLUT1 overexpression. The switch is achieved by directly increasing the expression of numerous glycolytic enzymes that are isoforms of those found in non-malignant cells.
Cracked, Porous Rocks and Fluids: Moon and Earth Paradox

Elastic wave velocities are key parameters in geosciences. In seismology at a large scale, or in seismic exploration at a more local and shallower scale, they were the main source of information for a long time. At the time of the Apollo mission, Anderson explained the unexpected result of very low velocities in Moon surface rocks by an intense cracking resulting from meteoritic impacts. Yet, it was also known that the Q factor was high. This could appear as a paradox. In the shallow layers of the Earth, rocks are porous. These shallow layers are of major importance in the Earth since they contain fluids. This is why velocities are higher and Q values lower in the Earth’s shallow layers than in the Moon’s shallow layers. Cracks have a determining effect on elastic properties because they are very compliant. Fluids also play a key role. Combining poroelasticity and effective elasticity, two independent theories much developed since the time of the Apollo mission, makes it possible to revisit the contrasting results observed in the Moon case and in the Earth case. Experimental results obtained on cracked synthetic glass show that dry cracks result in a strong decrease in velocity. On the other hand, saturated porous limestones exhibit a strong frequency-dependent attenuation when thermally cracked. The presence of fluid is the key factor.
LACA: RELIABILITY GENERALIZATION 1 Running head: LACA: RELIABILITY GENERALIZATION A Reliability Generalization Study for a Multidimensional Loneliness Scale: The Loneliness and Aloneness Scale for Children and Adolescents

Research on the average reliability and factors that affect the reliability of loneliness scales has been restricted to unidimensional measures. A reliability generalization (RG) study was conducted for a multidimensional loneness measure, that is, the Loneliness and Aloneness Scale for Children and Adolescents (LACA). Multilevel meta-analyses were performed on 79 studies that comprised 92 samples (for a total of 41,076 participants). Average reliability (Cronbach’s alpha) across samples was good (i.e., .80 or above) for all four subscales. Studies with higher sampling quality yielded slightly higher alphas for one of the subscales (i.e., Parent-related loneliness). For adolescents, as compared to children, alphas were somewhat lower for three of the four subscales and higher for the Affinity for aloneness subscale. Suggestions for future research are outlined. From a reliability perspective, the LACA is a good option for researchers who want to use a multidimensional loneliness measure with children and adolescents.
Using Bibliometric Methods to Shed Light on the Concept of Sustainable Tourism

The publication of the Brundtland report in 1987 introduced the concept of sustainable development, placing “sustainability” as a unifying idea for all academic disciplines. Consequently, in the tourism sector, sustainable tourism emerged as a new approach to research in the field. Since then, the body of literature on sustainable tourism has only increased, as has the discussion around the conceptual structure of sustainable tourism among academics and professionals. The aim of the study is to complement this theoretical discussion with an inductive approach to the limits of the conceptual structure of sustainable tourism. To do so, we identify the main research topics in the field and their evolution in the past 32 years through bibliometric methods such as evaluative techniques, relational techniques, and visualization of bibliometric data techniques using the VOSviewer program. The results of the study reflect that the sustainable tourism has reached a complexity that is reflected in its current polyhedral content to function as a conceptual umbrella. In addition, it demonstrates that the bibliometric analysis is an adequate and useful methodology for academics and professionals involved in the academic debate around this field of knowledge.
Current State of Semi-Solid Net-Shape Die Casting

Semi-solid processing of alloys is nearing the end of its fifth decade in existence. This promising manufacturing route has undergone a number of changes over the past five decades and apart from certain successful industrial applications, it appeared that it had become a niche technology with limited applications, notably in the automotive and consumer product markets. Nevertheless, despite the strong competition of traditional casting and in particular die-casting processes, and the emergence of Additive Manufacturing (AM), there seems to be a resurgence in demand for Net-Shape Die Casting of Semi-solid alloys as testified by the high numbers of delegates at the 15th SSM Conference at Shenzhen, China in September 2018. What follows is a brief review of the historical development of the process as well as more recent developments with an eye to future prospects.
C O ] 1 8 O ct 2 01 5 Induced cycles in triangle graphs

The triangle graph of a graph G, denoted by T (G), is the graph whose vertices represent the triangles (K3 subgraphs) of G, and two vertices of T (G) are adjacent if and only if the corresponding triangles share an edge. In this paper, we characterize graphs whose triangle graph is a cycle and then extend the result to obtain a characterization of Cn-free triangle graphs. As a consequence, we give a forbidden subgraph characterization of graphs G for which T (G) is a tree, a chordal graph, or a perfect graph. For the class of graphs whose triangle graph is perfect, we verify a conjecture of the third author concerning packing and covering of triangles.
FEA of tyres subjected to static loading

Structural static finite element analysis (FEA) may be used in tyre design, in order to predict and improve mechanical behaviour and durability of tyres. This paper describes the possible goals of static tyre FEA and gives special attention to various aspects of finite element (FE) model building. A short review of relevant papers is given first, followed by the description of axisymmetric and 3D models built by the authors and the results of analyses conducted using those. The accompanying comments describe how those results may be used by tyre designers.
Visual Scanning of Males and Females in Infancy

This study addressed the development of attention to information that is socially relevant to adults by examining infants' (n = 64) scanning patterns of male and female bodies. Infants exhibited systematic attention to regions associated with sex-related scanning by adults, with 3.5-and 6.5month-olds looking longer at the torso of females than males and longer at the legs of males than females. However, this pattern of looking was not found when infants were tested on headless bodies in Experiment 2, which suggests that infants' differential gaze pattern in Experiment 1 was not due to low-level stimulus features, such as clothing, and also indicates that facial/head information is necessary for infants to exhibit sex-specific scanning. We discuss implications for models of face and body knowledge development.
SUSCEPTIBILITY OF GRAPEVINE CULTIVARS AND ROOTSTOCKS TO CROWN GALL DISEASE (RHIZOBIUM VITIS) IN THE AEGEAN REGION OF TURKEY

Crown or root gall of grape is one of the most important bacterial diseases of vineyards in Turkey. Eighty-one vineyards in the Aegean Region were surveyed and 31 gall samples were collected between 2009 and 2011. A total of 16 of R. vitis isolates were identified and classified by PCR using speciesand opine type-specific primers. Pathogenicity was determined by inoculating plants of sunflower and a highly virulent strain was used to determine the sensitivity of the most common rootstocks (10) and grapevine cultivars (31) in Turkey for two years. Two-year old rooted grape cutiings were artificially inoculated with bacterial suspension (107cfu/ml). Plants were maintained in climate rooms (at 18-24°C temperature, 80% relative humidity, 12 h light/dark) and gall diameters and weights were measured after 3 months. At the end of the study, all the isolates obtained from this region were determined to cause octopin/cucumopine type opin synthesis. Ramsey and 1613-C were the most tolerant rootstocks while 420A and 41-B were the most susceptible to R. vitis. On the other hand, Sultan 1, Sultana Seedless (Type 4) and Sultan-7 (from the Sultana Seedless group) and Mevlana, Red Globe and Pembe Gemre (6th, 11th and 12th clones) were found to be the most susceptible while Bornova Misketi was the most tolerant grape cultivar.
Quantitative Proteomic Analysis Uncovers the Mediation of Endoplasmic Reticulum Stress-Induced Autophagy in DHAV-1-Infected DEF Cells

Autophagy is a tightly regulated catabolic process and is activated in cells in response to stress signals. Despite extensive study, the interplay between duck hepatitis A virus type 1 (DHAV-1) and the autophagy of host cells is not clear. In this study, we applied proteomics analysis to investigate the interaction mechanism between DHAV-1 and duck embryo fibroblast (DEF) cells. In total, 507 differentially expressed proteins (DEPs) were identified, with 171 upregulated proteins and 336 downregulated proteins. The protein expression level of heat shock proteins (Hsps) and their response to stimulus proteins and zinc finger proteins (ZFPs) were significantly increased while the same aspects of ribosome proteins declined. Bioinformatics analysis indicated that DEPs were mainly involved in the “response to stimulus”, the “defense response to virus”, and the “phagosome pathway”. Furthermore, Western blot results showed that the conversion of microtubule-associated protein 1 light chain 3-I (LC3-I) to the lipidation form of LC3-II increased, and the conversion rate decreased when DEF cells were processed with 4-phenylbutyrate (4-PBA). These findings indicated that DHAV-1 infection could cause endoplasmic reticulum (ER) stress-induced autophagy in DEF cells, and that ER stress was an important regulatory factor in the activation of autophagy. Our data provide a new clue regarding the host cell response to DHAV-1 and identify proteins involved in the DHAV-1 infection process or the ER stress-induced autophagy process.
AN EFFECT OF COMBINATION WITH ARTHROCENTESIS AND STABILIZATION SPLINT TREATMENT ON TEMPOROMANDIBULAR JOINT DISORDER PATIENT

The purpose of this study was to evaluate the clinical outcomes of patients with temporomandibular joint disorder before and after performance of arthrocentesis and stabilization splint therapy. The subjects of this study were 33 patients with limited mouth opening or pain or joint effusion who visited the Department of Oral and Maxillofacial Surgery, St Mary's Hospital, Catholic University of Korea. The arthrocentesis was performed and the stabilization splint was worn immediately after the arthrocentesis. Comparing the clinical outcomes of treatment at the times of first medical examination, 3 months and 6 months later on treatment. The results are as follows; 1. Statistically significant increase in the amount of maximum mouth opening occurred 3 months and 6 months later on treatment. 2. Statistically significant decrease in the average value of pain on test measured by VAS during maximum mouth opening, protrusive movement, right lateral movement, left lateral movement occured 3 months and 6 months later on treatment. The results suggested that arthrocentesis and stabilization splint therapy provide a improvement on patients with temporomandibular joint disorder when the exact diagnosis were provided.
CHARRON: CODE FOR HIGH ANGULAR RESOLUTION OF ROTATING OBJECTS IN NATURE∗

Rotation is one of the fundamental physical parameters governing stellar physics and evolution. At the same time, spectrally resolved optical/IR long-baseline interferometry has proven to be an important observing tool to measure many physical effects linked to rotation, in particular, stellar flattening, gravity darkening, differential rotation. In order to interpret the high angular resolution observations from modern spectro-interferometers, such as VLTI/AMBER and VEGA/CHARA, we have developed an interferometryoriented numerical model: CHARRON (Code for High Angular Resolution of Rotating Objects in Nature). We present here the characteristics of CHARRON, which is faster (' 10 − 30 s per model) and thus more adapted to model-fitting than the first version of the code presented by Domiciano de Souza et al. (2002).