Miracle-dz/UniFAR-FTUs huggingface dataset:
A novel Transposable element‐derived microRNA participates in plant immunity to rice blast disease

Summary MicroRNAs (miRNAs) are small non‐coding RNAs that direct post‐transcriptional gene silencing in plant development and stress responses through cleavage or translational repression of target mRNAs. Here, we report the identification and functional characterization of a new member of the miR812 family in rice (named as miR812w) involved in disease resistance. miR812w is present in cultivated Oryza species, both japonica and indica subspecies, and wild rice species within the Oryza genus, but not in dicotyledonous species. miR812w is a 24nt‐long that requires DCL3 for its biogenesis and is loaded into AGO4 proteins. Whereas overexpression of miR812w increased resistance to infection by the rice blast fungus Magnaporthe oryzae, CRISPR/Cas9‐mediated MIR812w editing enhances disease susceptibility, supporting that miR812w plays a role in blast resistance. We show that miR812w derives from the Stowaway type of rice MITEs (Miniature Inverted‐Repeat Transposable Elements). Moreover, miR812w directs DNA methylation in trans at target genes that have integrated a Stowaway MITE copy into their 3′ or 5′ untranslated region (ACO3, CIPK10, LRR genes), as well as in cis at the MIR812w locus. The target genes of miR812 were found to be hypo‐methylated around the miR812 recognition site, their expression being up‐regulated in transgene‐free CRISPR/Cas9‐edited miR812 plants. These findings further support that, in addition to post‐transcriptional regulation of gene expression, miRNAs can exert their regulatory function at the transcriptional level. This relationship between miR812w and Stowaway MITEs integrated into multiple coding genes might eventually create a network for miR812w‐mediated regulation of gene expression with implications in rice immunity.
Controllable release of pirfenidone by polyvinyl alcohol film embedded soft contact lenses in vitro and in vivo

Abstract To increase the amount of pirfenidone (PFD) loaded in polyvinyl alcohol (PVA) film embedded soft contact lens (SCL), and evaluate its function of sustaining delivery of drug in vitro and in vivo. Drug loading efficiency within PVA film and SCLs, drug release from SCLs in vitro, and the effects of parameters of SCLs and external environment on drug release in vitro were evaluated by ultraviolet–visible spectrophotometer at 312 nm. Safety of SCLs was evaluated in vitro by transformed human corneal epithelial cell. Safety in vivo was determined by optical coherence tomography and histology of anterior segment of rabbits. Drug release study in tear fluid and aqueous humor were measured by ultra-performance liquid chromatography. SCLs had smooth surface and were fit for experimental rabbits. Amount of PFD in PVA film and SCLs were 153.515 μg ± 12.508 and 127.438 μg ± 19.674, respectively, PFD in PVA film was significantly higher than SCLs (p=.006) and closed to 150 μg (targeting amount of PFD to be loaded). Thickness of SCLs, molecular weight of PVA, and amount of PVA used in SCLs affected drug release in vitro significantly. Thickness of PVA film and amount of drug in SCLs had no effect on drug release rate in vitro. SCLs were safe in vitro and in vivo, PFD released from SCLs could be detected around 12 hours in tears and aqueous humor, and the concentration of drug was higher than eye drop at all detected time points while amount of PFD in SCLs was lower than eye drop. Drug loaded PVA film embedded SCLs may be a promising ocular drug delivery system.
Pressure- and temperature-induced Raman spectral changes of 1-butyl-3-methylimidazolium tetrafluoroborate

In the present study, we have investigated the pressure- and temperature-induced Raman spectral changes of 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]). [bmim][BF4] did not crystallize at high pressures below 1.4 GPa. Upon compression, spectral changes in the band due to the CH stretching mode of the alkyl chain are larger than those due to the imidazolium ring, indicating that the environment around the alkyl chain is significantly perturbed. On the other hand, the Raman spectral change of [bmim][BF4] upon cooling at ambient pressure is very small and no crystallization occurs down to 113 K.
Do Women Require Less Permanent Pacemaker After Transcatheter Aortic Valve Implantation? A Meta‐Analysis and Meta‐Regression

Background Limited clinical evidence and literature are available about the potential impact of sex on permanent pacemaker implantation (PPI) after transcatheter aortic valve implantation (TAVI). The aim of this work was to evaluate the relationship between sexes and atrioventricular conduction disturbances requiring PPI after TAVI. Methods and Results Data were obtained from 46 studies from PubMed reporting information about the impact of patient sex on PPI after TAVI. Total proportions with 95% Cls were reported. Funnel plot and Egger test were used for estimation of publication bias. The primary end point was 30‐day or in‐hospital PPI after TAVI, with odds ratios and 95% CIs extracted. A total of 70 313 patients were included, with a cumulative proportion of 51.5% of women (35 691 patients; 95% CI, 50.2–52.7). The proportion of women undergoing TAVI dropped significantly over time (P<0.0001). The cumulative PPI rate was 15.6% (95% CI, 13.3–18.3). The cumulative rate of PPI in women was 14.9% (95% CI, 12.6–17.6), lower than in men (16.6%; 95% CI, 14.2–19.4). The risk for post‐TAVI PPI was lower in women (odds ratio, 0.90; 95% CI, 0.84–0.96 [P=0.0022]). By meta‐regression analysis, age (P=0.874) and ventricular function (P=0.302) were not significantly associated with PPI among the sexes. Balloon‐expandable TAVI significantly decrease the advantage of women for PPI, approaching the same rate as in men (P=0.0061). Conclusions Female sex is associated with a reduced rate of PPI after TAVI, without influence of age or ventricular function. Balloon‐expandable devices attenuate this advantage in favor of women. Additional investigations are warranted to elucidate sex‐based differences in developing conduction disturbances after TAVI.
Dalteparin and Rivaroxaban Sequential Use in Cancer Patients with Venous Thromboembolism.

OBJECTIVE To determine the effect and safety of sequential treatment with the low-molecular-weight heparin dalteparin and the direct oral anticoagulants rivaroxaban in patients with cancer- associated venous thromboembolism (VTE). STUDY DESIGN Observational study. PLACE AND DURATION OF STUDY Department of Oncology, the Second Affiliated Hospital of Soochow University, between January 2017 and September 2019. METHODOLOGY Patients with active cancer, diagnosed with VTE and who received sequential treatment with dalteparin and rivaroxaban, were retrospectively reviewed. Logistic regression analysis was used to identify risk factors associated with VTE recurrence. RESULTS Ninety-nine patients with active cancer were enrolled in the study. The median delteparin treatment time was nine days (5-20 days), and 2.8 months (1-6 months) for rivaroxaban. Sixty (60.6%) patients had eliminated VTE, and 39 (39.4%) had persistent VTE, but with relieved symptoms. No major bleeding was observed. Eleven (11.1%) patients had minor bleeding, including melena (5.1%), hematuria (3.0%), vaginal bleeding (1.0%), gingival bleeding (1.0%), and subcutaneous hemorrhage (1.0%). During the 6 months follow-up period, one (1.0%) developed pulmonary embolism, and seven (7.1%) experienced recurrent VTE. Univariate logistic regression analysis showed that bleeding occurrence and anticoagulant treatment duration were the two significant factors affecting VTE recurrence (p<0.05). CONCLUSION Maintenance of rivaroxaban after initial dalteparin treatment could effectively reduce the risk of VTE recurrence and was well tolerated by patients with cancer-associated VTE. However, in the clinical practice, the treatment duration is often insufficient, so it is essential to follow-up these patients to ensure sufficient treatment time. Key Words: Venous thromboembolism, Low-molecular-weight heparins, Directly oral anticoagulants, Cancer.
EVIDENCE NEEDS, TRAINING DEMANDS, AND OPPORTUNITIES FOR KNOWLEDGE TRANSLATION IN SOCIAL SECURITY AND INSURANCE MEDICINE: A EUROPEAN SURVEY

Objective To perform a European survey of the evidence needs and training demands of insurance medicine professionals related to professional tasks and evidence-based practice. Design International survey. Subjects Professionals working in insurance medicine. Methods Experts designed an online questionnaire including 26 questions related to 4 themes: evidence needs; training demands; evidence-seeking behaviour; and attitudes towards evidence-based medicine. Descriptive statistics were presented by country/conference and the total sample. Results A total of 782 participants responded. Three-quarter of participants experienced evidence needs at least once a week, related to mental disorders (79%), musculoskeletal disorders (67%) and occupational health (65%). Guidelines (76%) and systematic reviews (60%) were the preferred types of evidence and were requested for assessment of work capacity (64%) and prognosis of return-to-work (51%). Evidence-based medicine was thought to facilitate decision-making in insurance medicine (95%). Fifty-two percent of participants felt comfortable finding, reading, interpreting, and applying evidence. Countries expressed similar needs for reviews on typical topics. Conclusion This study reveals evidence gaps in key areas of insurance medicine, supporting the need for further research, guidelines and training in evidence-based insurance medicine. Importantly, insurance medicine professionals should recognize that evidence-based practice is crucial in producing high-quality assessments. LAY ABSTRACT Evidence-based medicine (EBM) means the consideration of research findings when taking decisions in clinical medicine. This approach has become standard in health and social care. Since it is less clear to what degree EBM is applied in insurance medicine, European insurance physicians performed a joint survey among colleagues in 8 countries and 2 conferences. More than 700 professionals participated. Half of respondents experienced a need at least once a week to look for guidelines and systematic reviews about work capacity assessments related to mental or musculoskeletal disorders. Almost all thought that EBM would facilitate decision-making. Not all felt competent in finding, reading, interpreting, and applying research evidence. Overall, findings were similar across countries. These results illustrate important evidence gaps in insurance medicine, supporting the need for further research and guidelines. Importantly, professionals should recognize that evidence-based practice is crucial in producing high-quality assessments.
The Biodegradation and Disintegration of Paper Products in the Composting Environment

The compostability of several paper products was determined according to the standards and criteria of the European standardization committee (CEN). The lignin content of the materials seems to be the main reason for the low biodegradability level achieved in the controlled composting test, which is a biodegradability test based on microbial degradation and measurement of carbon dioxide evolution. Mechanically pulped paper, which contains more lignin and more closely resembles natural wood than chemically bleached paper, generated smaller amounts of carbon dioxide. All the chemically bleached papers generated more than 70% of their organic carbon content as CO2, while the mechanically pulped unbleached papers in general generated less than 70%. Paper containing mechanical pulp degrades slower because lignin both interferes the biodegradation of cellulose and becomes incorporated in the humus. The test protocol also defines that, even if the biodegradability of paper products is not tested, their disintegration and effect on compost quality have to be determined. In the present study, we also evaluated a number of disintegration test methods in full-scale composting facilities. According to CEN, the disintegration can be studied either in composter bin-scale test equipment or in full-scale composting facilities. In this study, we only present the data from full-scale composting in which several test methods were used to evaluate the degradation of the samples. The disintegration of the paper products in full-scale composting facilities is highly dependent on the method used. The slowest degradation rate was achieved when the samples were composted in nylon bags.
Value of energy spectrum CT parameters in the differential diagnosis of high-grade clear cell renal cell carcinoma and type II papillary renal cell carcinoma

Background Energy spectrum computed tomography (CT) has become a promising approach for the differential diagnosis of tumor subtypes. Purpose To explore the value of energy spectrum CT parameters in the differential diagnosis of high-grade clear cell renal cell carcinoma (ccRCC) and type II papillary renal cell carcinoma (pRCC). Material and Methods Forty-two cases of high-grade ccRCC and 28 cases of type II pRCC were retrospectively reviewed. All region of interest (ROI) measurements were maintained consistently between the two-phase contrast-enhanced examinations. The ROIs encompassed as much of the enhancing areas of the lesions as possible. Energy spectrum CT parameters of all cases, including the 70 keV (HU) value, normalized iodine concentration (NIC), and energy spectrum curve slope were recorded by two radiologists with over 10 years of experience in abdominal CT diagnosis. Results In the cortical phase (CP) and parenchymal phase (PP), the 70 keV (HU) value, NIC, and slope value of the energy spectrum curve of high-grade ccRCC were significantly higher than those of type II pRCC. In the CP, NIC showed the highest differential diagnosis efficiency for the two group tumors, with a sensitivity of 78.9% and a specificity of 77.0%. There was no statistical difference in tumor hemorrhage, tumor envelope, tumor morphology, tumor border, lymph node metastasis, embolism, renal pelvis invasion, or tumor calcification between the two tumor types. However, there was significant difference in the number of tumors (P = 0.019). Conclusion Energy spectrum CT parameters are valuable for the differential diagnosis of high-grade ccRCC and type II pRCC.
Treatment target achievement after myocardial infarction and ischaemic stroke: cardiovascular risk factors, medication use, and lifestyle: the Tromsø Study 2015-16.

AIMS To investigate European guideline treatment target achievement in cardiovascular risk factors, medication use, and lifestyle, after myocardial infarction (MI) or ischaemic stroke, in women and men living in Norway. METHODS AND RESULTS In the population-based Tromsø Study 2015-16 (attendance 65%), 904 participants had previous validated MI and/or stroke. Cross-sectionally, we investigated target achievement for blood pressure (<140/90 mmHg, <130/80 mmHg if diabetes), LDL cholesterol (<1.8 mmol/L), HbA1c (<7.0% if diabetes), overweight (body mass index (BMI) <25 kg/m2, waist circumference women <80 cm, men <94 cm), smoking (non-smoking), physical activity (self-reported >sedentary, accelerometer-measured moderate-to-vigorous ≥150 min/week), diet (intake of fruits ≥200 g/day, vegetables ≥200 g/day, fish ≥200 g/week, saturated fat <10E%, fibre ≥30 g/day, alcohol women ≤10 g/day, men ≤20 g/day), and medication use (antihypertensives, lipid-lowering drugs, antithrombotics, and antidiabetics), using regression models. Proportion of target achievement was for blood pressure 55.2%, LDL cholesterol 9.0%, HbA1c 42.5%, BMI 21.1%, waist circumference 15.7%, non-smoking 86.7%, self-reported physical activity 79%, objectively measured physical activity 11.8%, intake of fruit 64.4%, vegetables 40.7%, fish 96.7%, saturated fat 24.3%, fibre 29.9%, and alcohol 78.5%, use of antidiabetics 83.6%, lipid-lowering drugs 81.0%, antihypertensives 75.9%, and antithrombotics 74.6%. Only 0.7% achieved all cardiovascular risk factor targets combined. Largely, there was little difference between the sexes, and in characteristics, medication use, and lifestyle among target achievers compared to non-achievers. CONCLUSION Secondary prevention of cardiovascular disease was suboptimal. A negligible proportion achieved the treatment target for all risk factors. Improvement in follow-up care and treatment after MI and stroke is needed.
Effect of orientation on ion track formation in apatite and zircon

Abstract Fission track (FT) thermochornology is essentially based on empirical fits to annealing data of FTs revealed by chemical etching, because, until now, unetched, latent FTs could not be examined analytically at the atomic-scale. The major challenge to such an analysis has been the random orientation of FTs and their extremely small diameters. Here we use high-energy ions (2.2 GeV Au or 80 MeV Xe) to simulate FT formation along specific crystallographic orientations. By combining results from transmission electron microscopy (TEM) of single tracks and small-angle X-ray scattering (SAXS) for millions of tracks, a precise picture of track morphology as a function of orientation is obtained. High-resolution analysis reveals that orientation affects the shape of tracks in apatite and zircon through the preferential creation of damage along directions with highest atomic density. However, track radius does not depend on orientation, contradicting previous reports. Independent of track orientation, track radii, as measured at each point along the entire length of 80 MeV Xe ion tracks in apatite, can be understood using the thermal spike model of Szenes. Thus, the well-known track annealing anisotropy of apatite is not due to track radius anisotropy. The combination of ion-irradiations with TEM and SAXS analysis provides a unique opportunity to understand and model track formation and annealing under various geologic conditions.
Thermally induced segregation of SnO2 nanoclusters in Sn-doped silica glasses from oversaturated Sn-doped silica xerogels

Silica glasses with substitutional Sn-doping or with dispersion of SnO2 nanoclusters were prepared by the sol–gel method. The occurrence of SnO2 clustering was investigated by optical absorption, Raman scattering and electron paramagnetic resonance spectroscopies, varying both the tin concentration and the densification temperature of Sn-doped silica xerogels. The 3.7 eV absorption edge of SnO2 and the A1g Raman mode at 630 cm−1 of the SnO2 phonon spectrum were used to assess the presence of SnO2 nanoclusters; the yield of X-ray induced paramagnetic E′ Sn centres was monitored to probe the variation in the amount of substitutional Sn centres. The results indicate that the clustering of SnO2 becomes effective for Sn concentrations ≥ 0.8 mol% and it is competitive with the substitutional doping in xerogels densified at T ≥ 900 °C. No evidence of pre-existent clustering was observed in xerogels densified at lower temperatures in the investigated doping range.
Pseudomonas aeruginosa biofilm killing beyond the spacer by antibiotic-loaded calcium sulfate beads: an in vitro study

Abstract Introduction: Bacterial biofilms are an important virulence factor in chronic periprosthetic joint infection (PJI) and other orthopedic infection since they are highly tolerant to antibiotics and host immunity. Antibiotics are mixed into carriers such as bone cement and calcium sulfate bone void fillers to achieve sustained high concentrations of antibiotics required to more effectively manage biofilm infections through local release. The effect of antibiotic diffusion from antibiotic-loaded calcium sulfate beads (ALCS-B) in combination with PMMA bone cement spacers on the spread and killing of Pseudomonas aeruginosa Xen41 (PA-Xen41) biofilm was investigated using a “large agar plate” model scaled for clinical relevance. Methods: Bioluminescent PA-Xen41 biofilms grown on discs of various orthopedic materials were placed within a large agar plate containing a PMMA full-size mock “spacer” unloaded or loaded with vancomycin and tobramycin, with or without ALCS-B. The amount of biofilm spread and log reduction on discs at varying distances from the spacer was assessed by bioluminescent imaging and viable cell counts. Results: For the unloaded spacer control, PA-Xen41 spread from the biofilm to cover the entire plate. The loaded spacer generated a 3 cm zone of inhibition and significantly reduced biofilm bacteria on the discs immediately adjacent to the spacer but low or zero reductions on those further away. The combination of ALCS-B and a loaded PMMA spacer greatly reduced bacterial spread and resulted in significantly greater biofilm reductions on discs at all distances from the spacer. Discussion: The addition of ALCS-B to an antibiotic-loaded spacer mimic increased the area of antibiotic coverage and efficacy against biofilm, suggesting that a combination of these depots may provide greater physical antibiotic coverage and more effective dead space management, particularly in zones where the spread of antibiotic is limited by diffusion (zones with little or no fluid motion).
) 0 6 5 GRS 1758-258 as a winged microquasar

A deep radio image of the microquasar GRS 1758-258 has evidenced for the first time a new link with the so called ‘winged’ radio galaxies. This opens a new perspective for the understanding of these secondary lobe features in relativistic jets. The fact that we also find them in a relativistic stellar outflow is better understood in the context of hydrodynamic models. This is in contrast to other alternative interpretations that are mostly based on the spin-flip of the central black hole in radio galaxies.
Rheological Properties and Stability of Emulsion Explosive Matrix

An investigation was performed into the rheological properties and stability of emulsion explosive matrix with different surfactants. The surfactants were oligomers of the polyisobutylene succinic anhydride (PIBSA) based on different head group and relative low molecular weight sorbitan monooleate (Span 80). The viscosity, shear stress, and storage modulus of emulsion explosive matrix depend on the nature of surfactants. It was shown that the matrix formed by Span 80 has a lower viscosity, shear stress, and storage modulus, compared with the PIBSA-based surfactants, especially the blended surfactant of Span 80 and PIBSA-TEA. The result of the precipitation of ammonium nitrate (AN) and the change of storage modulus with time show that the strength of interfacial film formed by Span 80 is weak. This allows the crystal of AN to pass through the interfacial film more easily; thus, the stability of the matrix is decreased. However, the storage modulus in aging was approximately constant and the ratio of the precipitation of AN is the lowest when the matrix was stabilized by blended surfactant. It shows that the matrix formed by blended surfactant has a better stability than single surfactants. GRAPHICAL ABSTRACT
Indicadores naturais de pH: usar papel ou solução?

In this work, the fruit extracts of Morus nigra - mulberry, Syzygium cuminii - jambolao, Vitis vinifera ¾ grape, Myrciaria cauliflora - jabuticaba are suggested as pH indicators in the form of either solutions or paper. The pH indicator solutions were prepared by soaking the fruits or their peels in ethanol 1:3 (m/V) for 24 h, followed by simple filtration. The pH indicator papers were prepared by imersion of the qualitative filter paper strips in the pH indicator solutions. The different pH leads to color changes in the indicator solutions or papers and it can be used for teaching elementary chemistry concepts.