Miracle-dz/UniFAR-FTUs huggingface dataset:
Dynamic Polyphosphate Metabolism Coordinating with Manganese Ions Defends against Oxidative Stress in the Extreme Bacterium Deinococcus radiodurans

The Mn-phosphate complex (Mn-Pi) plays a key role in the cellular resistance of radioresistant bacteria. The evolutionarily ancient polyphosphate polymers (polyphosphate [PolyP]) could effectively chelate Mn2+ and donate phosphates. ABSTRACT Deinococcus radiodurans is an extreme bacterium with unparalleled resistance to oxidative stresses. Accumulation of intracellular Mn2+ complexing with small metabolites is the key contributor to the tolerance of D. radiodurans against oxidative stress. However, the intracellular reservoir of Mn ions and homeostatic regulation of the Mn complex in D. radiodurans remain unclear. We identified an evolutionarily ancient and negatively charged phosphate polymer (polyphosphate [PolyP]) in D. radiodurans. We investigated PolyP metabolism in the response of D. radiodurans to oxidative stress. The genes dr1939, encoding polyphosphatase kinase (PPKDr; the subscript “Dr” refers to D. radiodurans), and dra0185, encoding exopolyphosphatase (PPXDr), were identified. PPXDr is a novel exopolyphosphatase with a cofactor preference to Mn2+, which enhances the dimerization and activity of PPXDr to allow the effective cleavage of PolyP-Mn. PPKDr and PPXDr exhibited different dynamic expression profiles under oxidative stress. First, ppkDr was upregulated leading to the accumulation of PolyP, which chelated large amounts of intracellular Mn ions. Subsequently, the expression level of ppkDr decreased while ppxDr was substantially upregulated and effectively hydrolyzed inactive PolyP-Mn to release phosphate (Pi) and Mn2+, which could form into Mn-Pi complexes to scavenge O2− and protect proteins from oxidative damage. Hence, dynamic cellular PolyP metabolites complexed with free Mn ions highlight a defense strategy of D. radiodurans in response to oxidative stress. IMPORTANCE The Mn-phosphate complex (Mn-Pi) plays a key role in the cellular resistance of radioresistant bacteria. The evolutionarily ancient polyphosphate polymers (polyphosphate [PolyP]) could effectively chelate Mn2+ and donate phosphates. However, the intracellular reservoir of Mn ions and homeostatic regulation of the Mn-Pi complex remain unclear. Here, we investigated the relationship of PolyP metabolites and Mn2+ homeostasis and how they function to defend against oxidative stress in the radioresistant bacterium Deinococcus radiodurans. We found that PPXDr (the subscript “Dr” refers to D. radiodurans) is a novel exopolyphosphatase with a cofactor preference for Mn2+, mediating PolyP-Mn degradation into Pi and Mn ions. The formed Mn-Pi complexes effectively protect proteins. The dynamic PolyP metabolism coordinating with Mn ions is a defense strategy of D. radiodurans in response to oxidative stress. The findings not only provide new insights into the resistance mechanism of the extreme bacterium D. radiodurans but also broaden our understanding of the functions of PolyP metabolism in organisms.
Effects of Boron and Paclobutrazol on Growth, Fruit Set, Nutrient Uptake, and Alternate Bearing of Muye Red Bayberry

Alternate bearing, resulting mainly from boron (B) deficiency, and excessive fruits in the alternate years of red bayberry trees are the most important limiting factors for production. This investigation was conducted on 18-year-old Muye red bayberry trees (Myrica rubra Sieb. et Zuca) in a commercial red bayberry orchard located in Laxi County, Zhejiang Province in 2006–2007.The results indicated that foliar application of paclobutrazol (PB) significantly increased the vegetative growth parameters (i.e., spring shoot incident and spring shoot length). Spring shoot incidents and spring shoot lengths of red bayberry were increased with increasing PB concentrations, but the optimum fruit yield and quality were obtained from foliar-applied PB at 100–200 mg L−1 at full bloom. Foliar application of PB increased the contents of N and K in the leaves with increases of PB concentrations up to 300–600 mg L−1. Fruit quality of red bayberry trees treated with PB or and boron fertilizer (BF) was improved at full bloom. Foliar application of PB reduced the percentage of fruit set by 62.1% in the year after treatment with PB but increased the yield by 49.0% the following year. Foliar application of PB at 100–300 mg L−1 gave the most significant improvement in fruit quality: increase in fruit weight, soluble solid content, and the ratio of soluble solids (SS) / total acidity (TA) and decrease in total acidity. Foliar application of BF slightly reduced the fruit yields in the current year but significantly increased those the following year. Combined application of BF and PB or application of PB alone markedly reduced the fruit yields in 2006 by 40.5% and 43.8%, respectively, but markedly increased those by 44.6% and 17.8% in 2007, respectively. The effects of alleviating alternate bearing of red bayberry for different treatments followed the order of combined application of FB + PB > PB > BF > control. It is suggested that PB and BF can be used to alleviate alternate bearing.
MODELING CHEMICAL EQUILIBRIUM OF ELECTROLYTE SOLUTIONS

ABSTRACT Modeling the chemical equilibrium in ionic solutions encountered in industrial applications, especially in the field of hydrometallurgy, still remains an unresolved issue. The complicated speciations, as well as the high ionic strengths encountered in these solutions, render the theories of analytical chemistry practically useless. The Debye–Hückel theory is examined in depth, so as to reveal the reasons of its failure. To remedy this problem, over the years, a large number of semi-empirical models have been proposed that are here reviewed, in order to help the reader find a model best suited for a system of interest. The models are classified into three main categories, based on their fundamental logic: ion interaction models describe the system through the physical interactions of the ions; ion association models describe the system through chemical equilibriums; and finally hybrid models use concepts from both previous categories. Focus is given in presenting the idea upon which each model is based rather than simply presenting the equations required for its implementation.
Evaluating the Effectiveness of Pre-Harvest Calcium Applications for Bitter Pit Control in ‘Golden Delicious’ Apples Under South African Conditions

ABSTRACT Pre-harvest foliar applications to increase fruit calcium (Ca) content and reduce bitter pit incidence, is a standard practice world wide. We re-evaluated the effectiveness of early season applications versus late applications of calcium nitrate (Ca(NO3)2) to reduce bitter pit in ‘Golden Delicious,’ under the present environmental conditions of the Western Cape, South Africa. Two periods of efficient uptake of external Ca were identified, viz., during cell division and the last few weeks before harvest. Foliar Ca applications during mid season (from 40 d after full bloom) were more effective in increasing fruit Ca content and reducing bitter pit incidence, than later applications. Thus, it is important to apply the bulk of the Ca(NO3)2 during the first few weeks after cell division to maximize the Ca content of ‘Golden Delicious’ apples.
Erectile Dysfunction and Cardiovascular Risk in Men With Rheumatoid Arthritis: A Population-based Cohort Study

Objective. Both erectile dysfunction (ED) and rheumatoid arthritis (RA) are associated with increased cardiovascular (CV) risk. It is unknown if these diagnoses are associated or if their combination confers additional CV risk. We aimed to define the incidence of ED in RA, and to determine if ED correlates with increased CV risk in RA. Methods. Medical information concerning RA, ED, and CV diagnoses for men with RA (n = 260) diagnosed in Olmsted County, Minnesota, and age-matched male comparators was extracted from a comprehensive medical record system. Results. ED incidence was similar between the RA cohort and comparators (HR 0.80, 95% CI 0.55–1.16). In men with RA, ED diagnosis was associated with a trend toward an increase in peripheral arterial disease (HR 2.22, 95% CI 0.98–5.03) and a significantly decreased rate of myocardial infarction (HR 0.26, 95% CI 0.07–0.90), heart failure (HR 0.49, 95% CI 0.25–0.94), and death (HR 0.56; 95% CI 0.36–0.87). In men with RA and ED, phosphodiesterase-5 inhibitor use was associated with a decreased risk of death (HR 0.35, 95% CI 0.16–0.79), with a trending decreased risk of some CV diagnoses. Conclusion. Incidence of ED was not statistically increased in RA. Although patients with both RA and ED had a similar overall CV risk to those with RA alone, men with both RA and ED had decreased risk of heart failure, myocardial infarction, and death, as well as an increased risk of peripheral arterial disease. Further studies are needed to clarify these associations and their implications for pathogenesis and therapeutics.
Discovery and Proof-of-Concept Study of Nuclease Activity as a Novel Biomarker for Breast Cancer Tumors

Simple Summary A diagnostic biomarker for the detection of breast cancer remains an unmet clinical need despite decades of intensive research efforts. Herein, we describe, for the first time, the use of nuclease activity as a biomarker to discriminate between healthy and cancer biopsy samples. We have identified a panel of three nucleic acid probes able to target nucleases derived from breast cancer tumors with high sensitivity and specificity. These results are in good agreement with histopathological analysis as the diagnostic gold standard. Moreover, these findings support nuclease activity as a potential adjacent diagnostic tool and shed light on the use of nuclease activity as a detection biomarker in breast cancer. Abstract Breast cancer is one of the most common pathologies diagnosed in the clinical practice. Despite major advancements in diagnostic approaches, there is no widely accepted biomarker in the clinical practice that can diagnose breast malignancy. Confirmatory diagnosis still relies on the pathological assessment of tissue biopsies by expert pathologists. Thus, there is an unmet need for new types of biomarkers and novel platform technologies that can be easily and robustly integrated into the clinic and that can assist pathologists. Herein, we show that nuclease activity associated to malignant tumors can be used as a novel biomarker in breast cancer, which can be detected via specific degradation of nucleic acid probes. In this study we have identified a set of three chemically modified nucleic acid probes that can diagnose malignancy in biopsy samples with high accuracy (89%), sensitivity (82%) and specificity (94%). This work represents a breakthrough for the potential clinical use of nuclease activity as biomarker, which can be detected via nucleic acids probes, for the clinical diagnosis of malignancy in breast tissue biopsies. This platform technology could be readily implemented into the clinic as adjunct to histopathological diagnostic.
The experiences of mothers who have a child diagnosed with cancer

Abstract Objective: This qualitative study explored the experiences of mothers who had a child diagnosed with cancer. Design and Outcome Measure: Informed by Interpretative Phenomenological Analysis, semi-structured interviews were completed with 13 participants, transcribed verbatim with individual and cross-case analysis conducted. Results: One superordinate theme, Protecting My Child, Whatever the Cost, was identified with five related subordinate themes. Participants battled to protect the development of their ill child. They richly described the personal costs and losses experienced, including putting their life on hold, and lost time with their healthy children. Participants faced realities of cancer treatment that were incongruent with their goal of protecting their child. Self-care awareness was significant for well-being as they protected themselves from reminders of their child’s mortality. Despite living in a crisis, participants reported changes suggestive of posttraumatic growth. Conclusion: Childhood cancer brings profound psychosocial and biographical disruption to the lives of mothers as they lose socially valued roles and have their identity as competent mothers challenged. Mothers protect their child, often at a cost to their health and well-being but also bringing positive consequences. The findings offer insights for psychologists in supporting mothers to reclaim their identity as competent mothers and renegotiate their mothering expectations.
The impact of demonstration plots on improved agricultural input purchase in Tanzania: Implications for policy and practice

In this paper, the authors use survey data from over 800 households to examine the impact of demonstration plots and associated activities (distribution of small packs of agricultural inputs) on smallholder farmers’ decisions to buy agricultural inputs in Tanzania. Using propensity score matching and inverse probability-weighted adjustment models, the authors estimated the effect of access to demonstration plots alone and demonstration plots combined with small packs of agricultural inputs on a household’s decision to purchase improved inputs. The results indicate that access to demonstration plots and demonstration plots with small packs increased the probability of purchasing improved inputs by 13–17 percentage points. This paper suggests that demonstration plots and demonstration plots with small packs are an effective model for enhancing improved technology adoption and are further increased when those inputs are available within a 5km radius. The results point to the importance of strengthening farmers’ organizations and last-mile agricultural input suppliers in order to enhance and facilitate access to information, appropriate production techniques, and improved inputs. The results also indicate the importance of investing in infrastructure to reduce transportation costs that limit market efficiency for appropriate technologies.
Exploring the Role of Personal Demands in the Health-Impairment Process of the Job Demands-Resources Model: A Study among Master Students

Research shows that students experience substantial levels of burnout during their studies. This study explores the role of personal demands on students’ well-being. After providing a conceptualization of personal demands, we examined the role of personal demands in the Job Demands-Resources (JD-R) model. Based on the Transactional Model of Stress, we hypothesized that students with high personal demands experience more burnout symptoms because they perceive more elements in their study as demanding (i.e., mediation hypothesis). At the same time, we hypothesized that the associations between study demands and burnout might be stronger for students with high versus low personal demands (i.e., moderation hypothesis). In order to test both hypotheses, we collected data from 578 master students. The data were analyzed with latent moderation and mediation analyses in Mplus. The results showed that students’ personal demands predicted burnout symptoms via the perception of study demands. Personal demands did not moderate the relationship between study demands and burnout. The findings of the present study expand the JD-R model by indicating that personal demands relate to burnout symptoms via the perception of study demands. Theoretical and practical implications are discussed.
Yield and Seed quality of barley (Hordeum vulgare L.) as affected by variety, nitrogen level and harvesting time

The experiment was conducted to find out the effect of variety, nitrogen level and harvesting time on yield and seed quality of barley. The treatments used in the experiment consisted of two varieties viz. BARI Barley 4 and BARI Barley 5, three harvesting time viz. 35, 40 and 45 Days after Anthesis (DAA) and nitrogen levels viz. 0, 70, 85 and 100 kg N ha -1 . The experiment was laid out in a spilt- spilt-plot design with three replications assigning the variety to the main plot, harvesting time to the sub-plots and nitrogen level to the sub-sub plots. Variety had significant effects on the all yield attributes except fertile seeds spike -1 . Seed quality parameters viz . normal seeds spike -1 , deformed seeds spike -1 , germination (%) and vigour index were statistically significant. The variety BARI Barley 5 produced higher grain yield and seed quality than BARI Barley 4. Grain yield from BARI Barley 5 and BARI Barley 4 were 4.59 t ha -1 and 4.24 t ha -1 , respectively. Significantly, the highest 1000-seed weight (46.90 g) was produced by BARI Barley 5 than (37.90 g) BARI Barley 4. The result revealed that harvesting time had significant effect on yield and yield attributes and seed quality parameters. Seed yield was highest (4.65 t ha -1 ) when the crop harvested at 40 DAA and it was increased linearly from 35 DAA. Maximum quality seed and 1000-seed weight (43.20 g) was obtained when the crop harvested at 40 DAA. All the yields, yield attributes and seed quality parameters were significantly influenced by nitrogen levels. The highest grain yield (5.14 t ha -1 ) was obtained when BARI Barley 5 variety was fertilized by 100 kg N ha -1 and the lowest (3.14 t ha -1 ) was obtained from control treatments. Normal seeds spike -1 , vigour index, germination (%) were better at 85 kg N ha -1 in variety of BARI Barley 5 than BARI Barley 4. So it can be concluded that BARI Barley 5 showed better result when fertilized with 100 kg N ha -1 and harvested at 40 DAA for getting maximum yield and 85 kg N ha -1 and harvested at 40 DAA for getting better quality seed.
Synthesis and characterisation of disulfides and esters derived from their sodium organodithiophosphonate salts

Reaction of Fc(S)PS2P(S)Fc and An(S)PS2P(S)An with NaOR [R=Me, Et, iPr] gives the non symmetric sodium phosphonodithioate salts Fc(RO)PS2Na and An(RO)PS2Na respectively (An=anisyl, Fc=ferrocenyl). These salts can be oxidised by I2, activated by KI, to form organodithiophosphono disulfides of the type (R(R′O)P(S)–S–S–P(S)(OR′)R). Reactions of these salts with 2,4-dinitrosulfenyl chloride form organodithiophosphono disulfides of the type [R(R′O)P(S)–S–S–R]. These sodium salts can also react with benzyl bromide to form S-alkyl O-alkyl 4-methoxyphenyldithiophosphonate esters and S-alkyl O-alkyl ferrocenyldithiophosphonate esters. All new compounds have been characterised spectroscopically and seven demonstrative X-ray structures are reported.
Pasting properties of potato starch and waxy maize starch mixtures.

The pasting viscosity, morphological properties, and swelling properties of potato starch and waxy maize starch mixtures at different ratios were investigated. Pasting analysis of the starch mixtures (7% solids in water, w/w) using a Rapid Visco Analyser showed linear changes in peak viscosity and pasting temperature according to the mixing ratios of both starches, but not in breakdown and setback. The pasting profile revealed that the starches rendered mutual effects during pasting, more significantly when the amounts of potato and waxy maize starches were similar. The volume fraction of swollen granules and the presence of amylose appeared to be important parameters in the mutual effects of both starched during pasting. Under a light microscope, the swelling of potato starch granules was delayed by the presence of waxy maize starch. Overall results indicate that new pasting properties can be generated by mixing starches of different botanical sources.
A Microencapsulation Method for Delivering Tetrodotoxin to Bivalves to Investigate Uptake and Accumulation

Most marine biotoxins are produced by microalgae. The neurotoxin tetrodotoxin (TTX) has been reported in many seafood species worldwide but its source is unknown, making accumulation and depuration studies in shellfish difficult. Tetrodotoxin is a water-soluble toxin and cannot be directly ingested by shellfish. In the present study, a method was developed which involved binding TTX to solid particles of humic acid and encapsulating them in agar-gelatin capsules. A controlled quantity of TTX-containing microcapsules (size range 20–280 μm) was fed to Paphies australis, a bivalve known to accumulate TTX in the wild. The TTX-containing microcapsules were fed to P. australis every second day for 13 days. Ten P. australis (including five controls fed non-toxic microalgae) were harvested after 7 days and ten after 13 days. Paphies australis accumulated TTX, reaching concentrations of up to 103 µg kg−1 by day 13, exceeding the European Food Safety Authority recommended concentration of 44 μg kg−1 in shellfish. This novel method will allow future studies to explore the effects, accumulation and depuration rates of TTX in different animals and document how it is transferred through food webs.
Antifungal Activity of a Medical-Grade Honey Formulation against Candida auris

Candida auris is a pathogenic yeast causing outbreaks in intensive care units with high mortality rates. The treatment of C. auris colonization is challenging due to high resistance rates. A potential alternative antifungal treatment is medical-grade honey. In this study the susceptibility of C. auris and other Candida species to the medical-grade honey-based formulation L-Mesitran® Soft was investigated. The medical-grade honey formulation reduced the growth of C. auris and other Candida species in a dose-dependent manner. This inhibition was not only due to the honey component, as treatment with an identical concentration of this component only was less effective and even stimulated the growth of C. albicans and C. glabrata, supporting the interpretation that supplements in the medical-grade honey formulation enhanced the antimicrobial activity. Increasing the concentration of the honey component to 40%, as is also present in an undiluted medical-grade honey formulation, lead to a 1- to 4-log inhibition of all Candida species. Unprocessed local honey reduced the growth of nearly all Candida species more strongly than medical-grade honey. C. auris’ susceptibility to the medical-grade honey formulation did not depend on geographic origin or resistance profile, although the multiresistant isolates tended to be more susceptible. Altogether, medical-grade honey formulation has a strong antifungal activity against C. auris and other Candida species. Future studies should demonstrate whether the treatment of open wounds or skin colonized with C. auris is feasible and effective in the clinical setting.
Reduced surround suppression in monocular motion perception

Motion discrimination of large stimuli is impaired at high contrast and short durations. This psychophysical result has been linked with the center-surround suppression found in neurons of area MT. Recent physiology results have shown that most frontoparallel MT cells respond more strongly to binocular than to monocular stimulation. Here we measured the surround suppression strength under binocular and monocular viewing. Thirty-nine participants took part in two experiments: (a) where the nonstimulated eye viewed a blank field of the same luminance (n = 8) and (b) where it was occluded with a patch (n = 31). In both experiments, we measured duration thresholds for small (1 deg diameter) and large (7 deg) drifting gratings of 1 cpd with 85% contrast. For each subject, a Motion Suppression Index (MSI) was computed by subtracting the duration thresholds in logarithmic units of the large minus the small stimulus. Results were similar in both experiments. Combining the MSI of both experiments, we found that the strength of suppression for binocular condition (MSIbinocular = 0.249 ± 0.126 log10 (ms)) is 1.79 times higher than under monocular viewing (MSImonocular = 0.139 ± 0.137 log10 (ms)). This increase is too high to be explained by the higher perceived contrast of binocular stimuli and offers a new way of testing whether MT neurons account for surround suppression. Potentially, differences in surround suppression reported in clinical populations may reflect altered binocular processing.