Miracle-dz/UniFAR-FTUs huggingface dataset:
It’s Not Easy Being Green: Behavior, Morphology, and Population Structure in Urban and Natural Populations of Green Anole (Anolis carolinensis) Lizards

Selection on behavior, signaling, and morphology can be strongly affected by variation in habitat type. Consequently, populations inhabiting different environments can exhibit divergent phenotypes as a result of either habitat-specific selection or plasticity. Urban habitats in particular represent different challenges for organisms adapted for rural environments, including disparate complements of predators and competitors, resource availability, and habitat complexity. In this paper, I review work aimed at understanding the different selective challenges experienced by rural and urban populations of green anole lizards, primarily those in southeastern Louisiana. I also describe a long-term mark-recapture experiment on an urban population of green anoles in New Orleans, and consider how sex ratios and population density changes over time. Collectively, this work shows that urban and rural populations of green anoles diverge markedly in behavior and morphology driven both by differences in habitat and the presence of competitors in the urban environment; however, it also shows that the effects of urbanization on the ecology and evolution of green anoles are understudied.
Responsiveness of Objective vs. Clinical Balance Domain Outcomes for Exercise Intervention in Parkinson's Disease

Background: Balance deficits in people with Parkinson's disease (PD) are often not helped by pharmacological or surgical treatment. Although balance exercise intervention has been shown to improve clinical measures of balance, the efficacy of exercise on different, objective balance domains is still unknown. Objective: To compare the sensitivity to change in objective and clinical measures of several different domains of balance and gait following an Agility Boot Camp with Cognitive Challenges (ABC-C) intervention. Methods: In this cross-over, randomized design, 86 individuals with PD participated in 6-week (3×/week) ABC-C exercise classes and 6-week education classes, consisting of 3–6 individuals. Blinded examiners tested people in their practical off state. Objective outcome measures from wearable sensors quantified four domains of balance: sway in standing balance, anticipatory postural adjustments (APAs) during step initiation, postural responses to the push-and-release test, and a 2-min natural speed walk with and without a cognitive task. Clinical outcome measures included the Unified Parkinson's Disease Rating Scale (MDS-UPDRS) Part III, the Mini Balance Evaluation Systems Test (Mini-BESTest), the Activities of Balance Confidence (ABC), and the Parkinson's Disease Questionnaire (PDQ-39). The standardized response means (SRM) of the differences between before and after each intervention compared responsiveness of outcomes to intervention. A linear mixed model compared effects of exercise with the active control—education intervention. Results: The most responsive outcome measures to exercise intervention with an SRM > 0.5 were objective measures of gait and APAs, specifically arm range of motion, gait speed during a dual-task walk, trunk coronal range of motion, foot strike angle, and first-step length at step initiation. The most responsive clinical outcome measure was the patient-reported PDQ-39 activities daily living subscore, but all clinical measures had SRMs <0.5. Conclusions: The objective measures were more sensitive to change after exercise intervention compared to the clinical measures. Spatiotemporal parameters of gait, including gait speed with a dual task, and APAs were the most sensitive objective measures, and perceived functional independence was the most sensitive clinical measure to change after the ABC-C exercise intervention. Future exercise intervention to improve gait and balance in PD should include objective outcome measures.
Immature Compost Suppresses Weed Growth Under Greenhouse Conditions

The influence of immature municipal solid waste-biosolids composts on emergence and mean days to emergence (MDE) of several weed species was evaluated in a pot trial under greenhouse conditions. The experiment consisted of placing a 7.5 cm deep layer of three-day-old immature compost, a mature and stable compost, an artificial medium or control sand as a mulch on ivyleaf morning glory seeds. Immature three-day-old compost decreased percentage emergence, shoot and root dry weight, and increased MDE of ivyleaf morning glory. In an experiment with eight-week-old immature compost utilizing mulching depths of 2.5, 5, 7.5, 10 cm and the untreated control on seeds of three weed species, common purslane did not emerge under any of the immature compost treatments. The MDE of ivyleaf morning glory and barnyard grass increased linearly as immature compost depths increased. Next, eight economically important weed species were sown in pots with either mature or immature (eight-week-old) compost utilizing mulching depths of 2.5 and 10 cm, in addition to an untreated control. Control pots yielded higher percentages of emergence than compost treatments for all species evaluated. Common purslane, large crabgrass, pig-weed, Florida beggarweed, and dichondra did not emerge through a 10-cm deep layer of mature compost mulch, or 2.5 or 10 cm deep layer of immature mulch. Significant compost maturity/depth interactions were observed for percent emergence on common purslane, ground cherry, large crabgrass, Florida beggarweed, and ivyleaf morning glory. A thinner layer was required to suppress germination using immature eight-week-old compost as compared to mature and stable compost. Immature (three-day or eight-week-old) compost containing acetic acid concentrations of 2474 and 1776 mg.kg−1 respectively reduced percentage emergence of several economically important weed species. These studies suggest that immature composts can be used to control weeds under conditions where spatial separation is maintained between the crop and the compost and phytotoxic fermentation products do not affect the health of the mulched plants and where odors associated with such partially stabilized products do not pose problems.
Functional morphology of the prosoma of Baltoeurypterus tetragonophthalmus (Fischer) (Chelicerata: Eurypterida)

Abstract The prosomal morphology of Baltoeurypterus tetragonophthalmus (Fischer) from the Baltic Silurian is redescribed and reconstructed. The first eurypterid labrum and new secondary sexual characters of Baltoeurypterus are described. The radially-arranged coxae of Baltoeurypterus were capable of adduction and abduction for food mastication, but not promotor-remotor movements for locomotion. Joint diagrams are presented for the first time for an extinct arthropod. Promotion and remotion of the limbs occurred about subvertical trochanteral pivots, as in all other chelicerates except xiphosurans. Baltoeurypterus probably walked in a “slow” gait; a method of choosing possible gaits for extinct arthropods is outlined. Swimming in Baltoeurypterus was effected by means of a rowing action of the posterior limb pair, which is provided with complex joints for collapsing the paddle during the recovery stroke. The limb arrangement and joint mechanisms of Baltoeurypterus are intermediate between those of the xiphosurans and the arachnids. It is possible that a sister relationship exists between the eurypterids and some arachnid groups, which would render Merostomata and Arachnida unnatural assemblages.
Extraction of Flavonoids From Natural Sources Using Modern Techniques

Flavonoids are one of the main groups of polyphenols found in natural products. Traditional flavonoid extraction techniques are being replaced by advanced techniques to reduce energy and solvent consumption, increase efficiency and selectivity, to meet increased market demand and environmental regulations. Advanced technologies, such as microwaves, ultrasound, pressurized liquids, supercritical fluids, and electric fields, are alternatives currently being used. These modern techniques are generally faster, more environmentally friendly, and with higher automation levels compared to conventional extraction techniques. This review will discuss the different methods available for flavonoid extraction from natural sources and the main parameters involved (temperature, solvent, sample quantity, extraction time, among others). Recent trends and their industrial importance are also discussed in detail, providing insight into their potential. Thus, this paper seeks to review the innovations of compound extraction techniques, presenting in each of them their advantages and disadvantages, trying to offer a broader scope in the understanding of flavonoid extraction from different plant matrices.
Sex and Age-class Differences in Vocalizations of Roosevelt Elk During Rut

Vocalizations of free-ranging Roosevelt elk (Cervus elaphus roosevelti) were studied at Gold Bluffs Beach, Prairie Creek Redwoods State Park, Humboldt Co., California, from August through November 1973. Significant differences were found in types and rates of elk vocalizations among different sex and age classes. Bull vocalizations varied with age and social status. Sonograph analyses revealed structural similarities in elk cohesion calls, bugles and yelps; bugles and yelps may be elaborations of cohesion calls. Bugling and yelping by master bulls occurred primarily in male-female encounters and may have functioned to bring cows closer together; bulls bugled most often when the harem was widely dispersed. Bulls vocalized less frequently in aggressive interactions between bulls where bugling and yelping clearly were related to male dominance. INTRODUCTION Despite extensive literature concerning mammalian vocalizations (Marler, 1955, 1967; Collias, 1960; Sebeok, 1968; Tembrock, 1968), comparatively little information exists for ungulates. Much of the recent literature on ungulate vocalizations was summarized by Kiley (1972), but quantitative descriptions of vocalizations and data on their causation were presented primarily for domestic species. Further, quantitative research on wild species often has focused on alarm vocalizations (Hirth and McCullough, 1977; Yahner, 1980; Richardson et al., 1983) or calls between mother and young (Espmark, 1971, 1975). Types and variability of vocalizations in other subspecies of Cervus elaphus have been well-documented (Murie, 1932, 1951; Darling, 1937; Johnson, 1951; Ahlen, 1965; Bubenik and Brna, 1967; Harper et al., 1967; Struhsaker, 1967; Olsen, 1979), and Peters (1980) reviewed the vocalizations of North American elk. Data on vocalizations of Roosevelt elk (C. e. roosevelti), however, were limited to qualitative descriptions (Graf, 1955; Harper et al., 1967), and information on the function and circumstances under which these sounds occurred was lacking. Further, Clutton-Brock and Albon (1979) noted that roaring by red deer (C. e. scoticus) stags occurred primarily during aggressive interactions between males, but roaring also was directed toward females in the stag's harem, suggesting a male-female function for this vocalization. Miura (1984) also noted that bugle-like "moans" of male sika (Cervus nippon) were given in response to the activities of females. The purpose of this study was to quantifv sex and age-class differences in vocalizations from a free-ranging population of Roosevelt elk during rut, and to examine the behavioral context in which vocalizations occurred. 'Present address: Institute of Arctic Biology, and Department of Biology and Wildlife, 211 Irving Building, University of Alaska, Fairbanks, Alaska 99775-1780.
Something New, Something Old: Parametric Models for the Size of Distribution of Income

Two new probability density functions (generalized beta and quadratic elasticity) are considered as models for the size distribution of income. The generalized beta distribution nests the generalized beta of the first and second kind, generalized gamma, lognormal and Pareto as well as introducing a number of new distributions to the literature. The quadratic elasticity distribution provides another generalization of the gamma distribution. The beta, gamma and lognormal distributions have been widely used in the income distribution literature. These distributions and many of their special cases are fit into five sets of US family income data for 1970, 1975, 1980, 1985, and 1990. This permits a comparison of the relative fit of several distributions over time.
Future Intentions of Fitness Center Customers: Effect of Emotions, Perceived Well-Being and Management Variables

One of the main objectives of fitness center managers is to obtain high levels of loyalty from the customers of these fitness centers. Within the existing literature on fitness center management, previous research has analyzed the importance of the management variables themselves to determine the behavioral intentions of their customers, ignoring other psychological and sociodemographic aspects and focusing on linear relationship models. Therefore, this study, which aims to analyze the impact of different management variables along with psychological (emotions and subjective well-being) and demographic variables (age and sex) on the satisfaction, perceived value (PV) and future intentions (FI) of 398 users (216 men, aged 18–75, Mean = 35.89 ± 14.53) of a fitness center, intends to fill this gap. In this study, two different methodologies are used, i.e., hierarchical regressions models (HRM) and qualitative comparative analysis (QCA). The data were obtained by means of a self-administered questionnaire composed of 69 items that collected different previously validated scales. Considering HRM, the different models proposed explain 52% of the satisfaction variance, 57% of perceived value and 59% of future intentions. In general, it seems that the management variables are better predictors than subjective well-being, emotions and age or gender since their inclusion does not greatly improve the model’s predictive capacity. As far as QCA analyses are concerned, it seems that none of the conditions are necessary for high or low levels of satisfaction, perceived value or future intentions. On the other hand, based on sufficiency analyses, there seem to be 8 pathways or combinations of conditions leading to high levels of satisfaction and 13 for low levels, 11 combinations leading to high levels of IF and 10 leading to low levels; however, there are 6 combinations of conditions leading to high levels of PV and 5 leading to low levels. In general, some of the pathways consider only the management variables, although many of them consider the importance not only of the management variables but also of the interactions that these may have with emotional aspects and, to a lesser extent, with age and well-being. When comparing both methodologies, it can be observed that the QCA models are more explanatory than the HRM models and that they take into account aspects that seem unimportant when observing linear models (such as emotions or age). However, both methodologies should be considered complementary and used simultaneously since, by focusing on different aspects, enriched results are obtained. The results obtained will enable managers to make more efficient use of available resources to increase user satisfaction.
Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species

Nucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions with the translocon complexes at the outer (TOC) and inner (TIC) plastid membranes. These complexes exist in multiple isoforms in higher plants and show differential specificity and tissue abundance. While some show specificity for photosynthesis-related precursor proteins, others distinctly recognize nonphotosynthetic and housekeeping precursor proteins. Here we used TPs from four Arabidopsis thaliana proteins, three related to photosynthesis (chlorophyll a/b binding protein, Rubisco activase) and photo-protection (tocopherol cyclase) and one involved in the assimilation of ammonium into amino-acids, and whose expression is most abundant in the root (ferredoxin dependent glutamate synthase 2), to determine whether they were able to mediate import of a nuclear-encoded marker protein into plastids of different tissues of a dicot and a monocot species. In A. thaliana, import and processing efficiency was high in all cases, while TP from the rice Rubisco small chain 1, drove very low import in Arabidopsis tissues. Noteworthy, our results show that Arabidopsis photosynthesis TPs also mediate plastid import in rice callus, and in leaf and root tissues with almost a 100% efficiency, providing new biotechnological tools for crop improvement strategies based on recombinant protein accumulation in plastids by the expression of nuclear-encoded transgenes.
Potentials and Challenges of Genomics for Breeding Cannabis Cultivars

Cannabis (Cannabis sativa L.) is an influential yet controversial agricultural plant with a very long and prominent history of recreational, medicinal, and industrial usages. Given the importance of this species, we deepened some of the main challenges—along with potential solutions—behind the breeding of new cannabis cultivars. One of the main issues that should be fixed before starting new breeding programs is the uncertain taxonomic classification of the two main taxa (e.g., indica and sativa) of the Cannabis genus. We tried therefore to examine this topic from a molecular perspective through the use of DNA barcoding. Our findings seem to support a unique species system (C. sativa) based on two subspecies: C. sativa subsp. sativa and C. sativa subsp. indica. The second key issue in a breeding program is related to the dioecy behavior of this species and to the comprehension of those molecular mechanisms underlying flower development, the main cannabis product. Given the role of MADS box genes in flower identity, we analyzed and reorganized all the genomic and transcriptomic data available for homeotic genes, trying to decipher the applicability of the ABCDE model in Cannabis. Finally, reviewing the limits of the conventional breeding methods traditionally applied for developing new varieties, we proposed a new breeding scheme for the constitution of F1 hybrids, without ignoring the indisputable contribution offered by genomics. In this sense, in parallel, we resumed the main advances in the genomic field of this species and, ascertained the lack of a robust set of SNP markers, provided a discriminant and polymorphic panel of SSR markers as a valuable tool for future marker assisted breeding programs.
Purinoceptor P2K1/DORN1 Enhances Plant Resistance Against a Soilborne Fungal Pathogen, Rhizoctonia solani

The purinoceptor P2K1/DORN1 recognizes extracellular ATP, a damage-associated molecular pattern (DAMP) released upon cellular disruption by wounding and necrosis, which in turn, boost plant innate immunity. P2K1 is known to confer plant resistance to foliar biotrophic, hemi-biotrophic, and necrotrophic pathogens. However, until now, no information was available on its function in defense against root pathogens. In this report, we describe the contribution of P2K1 to resistance in Arabidopsis against Rhizoctonia solani, a broad host range, necrotrophic soilborne fungal pathogen. In pot assays, the Arabidopsis P2K1 overexpression line OxP2K1 showed longer root length and a greater rosette surface area than wild type in the presence of the pathogen. In contrast, the knockout mutant dorn1-3 and the double mutant rbohd/f, defective in two subunits of the respiratory burst complex NADPH oxidase, exhibited significant reductions in shoot and root lengths and rosette surface area compared to wild type when the pathogen was present. Expression of PR1, PDF1.2, and JAZ5 in the roots was reduced in dorn1-3 and rbohd/f and elevated in OxP2K1 relative to wild type, indicating that the salicylate and jasmonate defense signaling pathways functioned in resistance. These results indicated that a DAMP-mediated defense system confers basal resistance against an important root necrotrophic fungal pathogen.
Pathomechanisms of Autoimmune Based Testicular Inflammation

Infection and inflammation of the male reproductive tract are relevant causes of infertility. Inflammatory damage occurs in the special immunosuppressive microenvironment of the testis, a hallmark termed testicular immune privilege, which allows tolerance to neo-antigens from developing germ cells appearing at puberty, long after the establishment of systemic immune tolerance. Experimental autoimmune orchitis (EAO) is a well-established rodent model of chronic testicular inflammation and organ specific autoimmunity that offers a valuable in vivo tool to investigate the pathological and molecular mechanisms leading to the breakdown of the testicular immune privilege. The disease is characterized by the infiltration of the interstitium by immune cells (mainly macrophages, dendritic cells, and T cells), formation of autoantibodies against testicular antigens, production of pro-inflammatory mediators such as NO, MCP1, TNFα, IL6, or activins and dysregulation of steroidogenesis with reduced levels of serum testosterone. EAO leads to sloughing of germ cells, atrophic seminiferous tubules and fibrotic remodeling, parameters all found similarly to changes in human biopsies from infertile patients with inflammatory infiltrates. Interestingly, testosterone supplementation during the course of EAO leads to expansion of the regulatory T cell population and inhibition of disease development. Knowledge of EAO pathogenesis aims to contribute to a better understanding of human testicular autoimmune disease as an essential prerequisite for improved diagnosis and treatment.
Maternal diabetes induces senescence and neural tube defects sensitive to the senomorphic rapamycin

A microRNA-activated pathway in maternal diabetes–induced neuroepithelial cell senescence leads to neural tube defects. Neural tube defects (NTDs) are the second most common structural birth defect. Senescence, a state of permanent cell cycle arrest, occurs only after neural tube closure. Maternal diabetes–induced NTDs are severe diabetic complications that lead to infant mortality or lifelong morbidity and may be linked to premature senescence. Here, we report that premature senescence occurs in the mouse neuroepithelium and disrupts neurulation, leading to NTDs in diabetic pregnancy. Premature senescence and NTDs were abolished by knockout of the transcription factor Foxo3a, the miR-200c gene, and the cell cycle inhibitors p21 and p27; transgenic expression of the dominant-negative FoxO3a mutant; or the senomorphic rapamycin. Double transgenic expression of p21 and p27 mimicked maternal diabetes in inducing premature neuroepithelium senescence and NTDs. These findings integrate transcription- and epigenome-regulated miRNAs and cell cycle regulators in premature neuroepithelium senescence and provide a mechanistic basis for targeting premature senescence and NTDs using senomorphics.
Blood indices and serum biochemical profile in ascites syndrome affected broilers

Studies with regard to blood indices and biochemical variables were made upon 45 ascitic and 30 apparently healthy birds procured from the fifteen ascites affected farms in Haryana during winter season. The affected birds exhibited a significant rise in haemoglobin (Hb), packed cell volume (PCV) and total erythrocyte count (TEC) while the erythrocyte indices viz. mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCR) and mean corpuscular haemoglobin concentration (MCHC) did not vary significantly from that of the controls. There was a significant fall in serum total proteins, albumin, albumin globulin ratio (A:G), calcium and sodium. The elevation of serum alanine aminotransferase, asparate aminotransferase, alkaline phofphates and phosphorus was reported in the ascitic birds. The results indicated haemoconcentration alongwith large fluctuations in serum enzyme and mineral levels in ascitic birds.