Miracle-dz/UniFAR-FTUs huggingface dataset:
Simple benchmarking method for determining the accuracy of depth cameras in body landmark location estimation: Static upright posture as a measurement example

To evaluate the postures in ergonomics applications, studies have proposed the use of low-cost, marker-less, and portable depth camera-based motion tracking systems (DCMTSs) as a potential alternative to conventional marker-based motion tracking systems (MMTSs). However, a simple but systematic method for examining the estimation errors of various DCMTSs is lacking. This paper proposes a benchmarking method for assessing the estimation accuracy of depth cameras for full-body landmark location estimation. A novel alignment board was fabricated to align the coordinate systems of the DCMTSs and MMTSs. The data from an MMTS were used as a reference to quantify the error of using a DCMTS to identify target locations in a 3-D space. To demonstrate the proposed method, the full-body landmark location tracking errors were evaluated for a static upright posture using two different DCMTSs. For each landmark, we compared each DCMTS (Kinect system and RealSense system) with an MMTS by calculating the Euclidean distances between symmetrical landmarks. The evaluation trials were performed twice. The agreement between the tracking errors of the two evaluation trials was assessed using intraclass correlation coefficient (ICC). The results indicate that the proposed method can effectively assess the tracking performance of DCMTSs. The average errors (standard deviation) for the Kinect system and RealSense system were 2.80 (1.03) cm and 5.14 (1.49) cm, respectively. The highest average error values were observed in the depth orientation for both DCMTSs. The proposed method achieved high reliability with ICCs of 0.97 and 0.92 for the Kinect system and RealSense system, respectively.
Perceptions on acceptability and reported consumption of marijuana by blood donors prior to donation in the recreational use state of Colorado, USA

Blood donor opinions and behaviours regarding marijuana use are not well known nor is the potential impact to the blood supply. We sought to assess opinions and frequency of marijuana use in proximity to blood donation via a survey of blood donors at a hospital‐based blood collection site in a state where recreational marijuana use has been legal since 2012.
Identification and functional prediction of long noncoding RNAs related to intramuscular fat content in Laiwu pigs

Objective Intramuscular fat (IMF) is a critical economic indicator of pork quality. Studies on IMF among different pig breeds have been performed via high-throughput sequencing, but comparisons within the same pig breed remain unreported. Methods This study was performed to explore the gene profile and identify candidate long noncoding RNA (lncRNAs) and mRNAs associated with IMF deposition among Laiwu pigs with different IMF contents. Based on the longissimus dorsi muscle IMF content, eight pigs from the same breed and management were selected and divided into two groups: a high IMF (>12%, H) and low IMF group (<5%, L). Whole-transcriptome sequencing was performed to explore the differentially expressed (DE) genes between these two groups. Results The IMF content varied greatly among Laiwu pig individuals (2.17% to 13.93%). Seventeen DE lncRNAs (11 upregulated and 6 downregulated) and 180 mRNAs (112 upregulated and 68 downregulated) were found. Gene Ontology analysis indicated that the following biological processes played an important role in IMF deposition: fatty acid and lipid biosynthetic processes; the extracellular signal-regulated kinase cascade; and white fat cell differentiation. In addition, the peroxisome proliferator-activated receptor, phosphatidylinositol-3-kinase-protein kinase B, and mammalian target of rapamycin pathways were enriched in the pathway analysis. Intersection analysis of the target genes of DE lncRNAs and mRNAs revealed seven candidate genes associated with IMF accumulation. Five DE lncRNAs and 20 DE mRNAs based on the pig quantitative trait locus database were identified and shown to be related to fat deposition. The expression of five DE lncRNAs and mRNAs was verified by quantitative real time polymerase chain reaction (qRT-PCR). The results of qRT-PCR and RNA-sequencing were consistent. Conclusion These results demonstrated that the different IMF contents among pig individuals may be due to the DE lncRNAs and mRNAs associated with lipid droplets and fat deposition.
Negative gravitropism of Ginkgo biloba: growth stress and reaction wood formation

Abstract Ginkgo (Ginkgo biloba L.) forms thick, lignified secondary xylem in the cylindrical stem as in Pinales (commonly called conifers), although it has more phylogenetic affinity to Cycadales than to conifers. Ginkgo forms compression wood-like (CW-like) reaction wood (RW) in its inclined stem as it is the case in conifers. However, the distribution of growth stress is not yet investigated in the RW of ginkgo, and thus this tissue resulting from negative gravitropism is still waiting for closer consideration. The present study intended to fill this gap. It has been demonstrated that, indeed, ginkgo forms RW tissue on the lower side of the inclined stem, where the compressive growth stress (CGS) was generated. In the RW, the micorofibril angle in the S2 layer, the air-dried density, and the lignin content increased, whereas the cellulose content decreased. These data are quite similar to those of conifer CWs. The multiple linear regression analysis revealed that the CGS is significantly correlated by the changes in the aforementioned parameters. It can be safely concluded that the negative gravitropism of ginkgo is very similar to that of conifers.
The correlation between the STOP-Bang score and oxygen saturation during spinal anesthesia with dexmedetomidine sedation

Background The STOP-Bang questionnaire is a simple screening tool with high sensitivity for the detection of severe obstructive sleep apnea. Predicting airway obstruction would allow the safe management of sedative patients to prevent intraoperative hypoxia. This study was designed to check the correlation between the STOP-Bang score and oxygen saturation (SpO2) during sedation and confirm the availability of the STOP-Bang questionnaire as a preoperative exam for predicting the incidence of hypoxia in sedative patient management. Methods This study included 56 patients who received spinal anesthesia. The pre-anesthesia evaluation was conducted using the STOP-Bang questionnaire. The patients were under spinal anesthesia with an average block level of T10. Dexmedetomidine was infused with a loading dose of 1 μg/kg over 10 min and a maintenance dose of 0.5 μg/kg/h until the end of the procedure. The SpO2 of the patients was recorded every 5 min. Results The STOP-Bang score was negatively correlated with the lowest SpO2 (coefficient = –0.774, 95% confidence interval [CI]: –0.855 to –0.649, standard error [SE] = 0.054, P < 0.001). The item of “observed apnea” was the most correlated one with hypoxic events (odds ratio = 6.00, 95% CI: 1.086 to 33.145). Conclusions The STOP-Bang score was significantly correlated with the lowest SpO2 during spinal anesthesia, which enabled the prediction of meaningful hypoxia before it occurred in the sedated patients.
Retinal Microvascular Alterations in Patients with Quiescent Posterior and Panuveitis Using Optical Coherence Tomography Angiography

ABSTRACT Purpose To quantify retinochoroidal vascular parameters using swept-source optical coherence tomography (SS-OCTA) in quiescent posterior and panuveitis. Methods In this cross-sectional study, subjects with quiescent posterior and panuveitis underwent fundus imaging using SS-OCTA (DRI Triton®, Topcon, Japan). The metrics calculated were fractal dimension (FD), foveal avascular zone (FAZ) area, retinal vascularity index (capillary density index-CDI), and choroidal vascularity index (CVI). Results We included 38 eyes of 20 patients, 9 males aged 34.7 ± 10.5 years, 30 eyes of 30 age- and gender-matched healthy controls, 10 females aged 33.6 ± 8.5 years. Comparing patients with controls, we found a lower FD (p < .001), larger FAZ (p > .001), lower CDI in the superficial plexus (p = .019), and lower CVI (p < .001). We also found lower retinal and choroidal and thicknesses (p < .001 and p = .025, respectively). Conclusions Patients with quiescent posterior and panuveitis have a significantly reduced retinochoroidal vascular density compared to healthy control subjects.
Is there a risk to honeybees from use of thiamethoxam as a sugar beet seed treatment?

The ban imposed by the European Union on the use of neonicotinoids as sugar beet seed treatments was based on the exposure of bees to residues of neonicotinoids in pollen and nectar of succeeding crops. To address this concern, residues of thiamethoxam (TMX) and clothianidin (CTD) were analyzed in soil collected from fields planted in at least the previous year with thiamethoxam‐treated sugar beet seed. This soil monitoring program was conducted at 94 sites across Germany in two separate years. In addition, a succeeding crop study assessed residues in soil, guttation fluid, pollen, and nectar sampled from untreated succeeding crops planted in the season after thiamethoxam seed‐treated sugar beet at eight field sites across five countries. The overall mean residues observed in soil monitoring were 8.0 ± 0.5 µg TMX + CTD/kg in the season after the use of treated sugar beet seed. Residue values decreased with increasing time interval between the latest thiamethoxam or clothianidin application before sugar beet drilling and with lower application frequency. Residues were detected in guttation fluid (2.0–37.7 µg TMX/L); however, the risk to pollinators from this route of exposure is likely to be low, based on the reported levels of consumption. Residues of thiamethoxam and clothianidin in pollen and nectar sampled from the succeeding crops were detected at or below the limit of quantification (0.5–1 µg a.i./kg) in 86.7% of pollen and 98.6% of nectar samples and, unlike guttation fluid residues, were not correlated with measured soil residues. Residues in pollen and nectar are lower than reported sublethal adverse effect concentrations in studies with honeybee and bumble bee individuals and colonies fed only thiamethoxam‐treated sucrose, and are lower than those reported to result in no effects in honeybees, bumble bees, and solitary bees foraging on seed‐treated crops. Integr Environ Assess Manag 2022;18:709–721. © 2021 SYNGENTA. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Chemical Composition, Antioxidant Capacity and Total Phenolic Content of the Flours Obtained from Cow Pea (Vigna ungualata) Varieties Commonly Consumed in Nigeria

Aim: To determine the proximate, mineral composition, antioxidant capacity and total phenolic content of the flours of four varieties of cow pea commonly consumed in Nigeria and to examine the influence of the colour of the beans’ hull on the antioxidant capacity of the bean varieties. Place and Duration of Study: The experiments on proximate and mineral composition were performed in Chemistry Laboratory of Ekiti State University, Ado-Ekiti while those on antioxidant capacity and total phenolic content were conducted in Chemistry Laboratory, Afe Babalola University, Ado-Ekiti, Nigeria. Methodology: Standard analytical methods were employed to determine the proximate and mineral composition of the flours obtained from four varieties of cow pea Vigna unguiculata subsp. dekindtiana, the wild relative of the cultivated subspecies; Vigna unguiculata subsp. cylindrica, cultivated, catjang; Vigna unguiculata subsp. sesquipedalis, cultivated, yardlong beans andVigna unguiculata subsp. unguiculata, cultivated, blackeyed pea. The antioxidant capacity and total phenolic content of the aqueous extracts were determined using Ferric ion Reducing Antioxidant potential (FRAP assay) and FolinOriginal Research Article British Journal of Applied Science & Technology, 4(12): 1729-1735, 2014 1730 Colcalteau method respectively. Results: The moisture content, crude fibre, ash and fat were generally low in the four varieties with significantly high protein (24.19%-25.74%). Calcium and phosphorous were found to be high in all the varieties. Antioxidant Activity ranged from 44.52-150.05 mgGAE/100g DW while the total phenolic content varied from 88.15-100.02 mgGAE/100 g DW. A linear positive relationship existed between the antioxidant activity and total phenolic content of the cow pea varieties (R=0.3333). Conclusion: This study shows that the four cow pea varieties could serve as good sources of protein, calcium and phosphorous with valuable antioxidant properties. All the varieties could however be utilized in food preparations and formulations for both adults and children.
A prospective population-based study of stroke in the Central Region of Iran: The Qom Incidence of Stroke Study

Objective Based on the few population-based studies that have been conducted in the Middle East, we determined the incidence of stroke in Qom, one of the central provinces of Iran. Methods The Qom province includes an estimated at-risk population of about 1 million. During a 12-month period (November 2018–November 2019), all first-ever strokes occurring in the target population were registered. Hospitalized cases were ascertained by discharge codes. Out-of-hospital cases were ascertained by a prospective screening of emergency medical services, emergency departments, ambulances records, primary care clinics, rural and urban public health centers, primary care physician offices, and neurologists’ offices. Crude and age-adjusted incidence rates (per 100,000 person-years) were calculated. Results During the study period, 1462 first-ever strokes occurred with a mean age of 68.1 (17–103) years; of these 45.2% were females (661 cases). The crude annual incidence rate per 100,000 at-risk populations was 145.4 (95% confidence interval, 138.1–153.0) for all types of stroke (156.5 for males and 134.3 for females), 26.4 (95% confidence interval, 23.5–29.8) for hemorrhagic stroke, and 114 (95% confidence interval, 105–121) for ischemic stroke. The incidence rate adjusted to the world population was 201.4 (95% confidence interval, 193–210) per 100,000 at-risk populations (adj incidence, 218.5 for males vs 187.4 for females). The total fatality rate during the first 28 days was 19.6%. Conclusion This study states that in this region there is a high incidence of stroke, which occurs at a younger age than the global average. There was a high prevalence of underlying stroke risk factors.
Environmental Correlates of Physical Activity, Sedentary Behavior, and Self-Rated Health in Chronic Obstructive Pulmonary Disease

The present study sought to investigate the potential relationship between the environment and physical activity in chronic obstructive pulmonary disease. Several associations were shown in the data that may assist clinicians with physical activity prescription in this clinical population. Purpose: Physical activity (PA) predicts important health outcomes in chronic obstructive pulmonary disease (COPD). In the general population, environmental factors have the potential to influence PA; however, data are limited in this clinical population. Therefore, we sought to investigate associations between the environment and PA, sedentary behavior, and self-rated health in COPD. Methods: Sociodemographic, PA, sedentary behavior, and self-rated health data were collected from a prospective cohort of 418 individuals with COPD (65% female; 58 ± 8 yr), while environmental data were drawn from a national environmental data repository and individually matched to participant postal code. Environmental variables included social and material deprivation, urban form index, surrounding greenness, and air quality (concentrations of air pollution for fine particles, nitrogen dioxide, ozone, and sulphur dioxide). Logistic and multivariate linear regression models were used to investigate the strongest environmental predictors. Results: The models showed a statistically significant negative correlation between PA level and ozone pollution (P = .023; adjusted OR = 0.85: 95% CI, 0.74-0.98). Urban form index was also significantly associated with sedentary behavior (β = 0.113; t value = 1.71; P = .011). Self-rated health was significantly positively correlated with PA level (P = .006; adjusted OR = 2.22: 95% CI, 1.25-3.94), and significantly inversely correlated with sedentary behavior (β = −0.159; t value =−2.42; P = .016). Conclusion: These new data may identify barriers to PA and assist clinicians in the prescription of exercise for individuals living with COPD.
The Diagnostic Value of Coagulation Indicators and Inflammatory Markers in Distinguishing Between Strangulated and Simple Intestinal Obstruction

Background: Intestinal obstruction with strangulation can be life-threating, and it is critical to make an accurate and timely diagnosis for emergency surgery. Objective: This was aimed to investigate the value of coagulation indicators and inflammatory markers in distinguishing between strangulated and simple intestinal obstruction. Materials and Methods: Fifty-four patients with intestinal obstruction were retrospectively studied. The correlation between coagulation indicators and inflammatory markers with intestinal obstruction was analyzed. Receiver operating characteristic curves were created to assess their ability in discriminative diagnosis. Results: Levels of fibrinogen (Fib), C-reactive protein (CRP), neutrophil ratio, and D-Dimer were significantly greater, while thrombin time was significantly shorter in strangulated intestinal obstruction compared with simple intestinal obstruction. Furthermore, Fib levels in the necrosis subgroup of strangulated intestinal obstruction were significantly higher than those in the ischemia subgroup and simple intestinal obstruction group. The areas under the receiver operating characteristic curve were 0.58 for white blood cells, 0.78 for CRP, and 0.80 for Fib. Using the optimal cutoff values of Fib (3.71 g/L) and CRP (14.54 mg/L), the sensitivity, specificity, positive predictive value, and negative predictive value in discriminating between strangulated intestinal obstruction and simple intestinal obstruction were 51.43%, 100%, 100%, and 52.78% for Fib, and 56.25%, 94.44%, 94.74%, and 54.84% for CRP, respectively. Conclusions: Fib and CRP demonstrate good performance in predicting strangulation and are indicative of intestinal necrosis and ischemia. The combination of this coagulation indicator and inflammatory marker holds potential for better discrimination between strangulated and simple intestinal obstruction.
Resting state dynamic functional connectivity in children with attention deficit/hyperactivity disorder

Attention deficit/hyperactivity disorder (ADHD) is characterized by inattention, hyperactivity and impulsivity. In this study, we investigated group differences in dynamic functional connectivity (dFC) between 113 children with inattentive (46 ADHDI) and combined (67 ADHDC) ADHD and 76 typically developing (TD) children using resting-state functional MRI data. For dynamic connectivity analysis, the data were first decomposed into 100 independent components, among which 88 were classified into eight well-known resting-state networks (RSNs). Three discrete FC states were then identified using k-means clustering and used to estimate transition probabilities between states in both patient and control groups using a hidden Markov model. Our results showed state-dependent alterations in intra and inter-network connectivity in both ADHD subtypes in comparison with TD. Spending less time than healthy controls in state 1, both ADHDI and ADHDC were characterized with weaker intra-hemispheric connectivity with functional asymmetries. In this state, ADHDI further showed weaker inter-hemispheric connectivity. The patients spent more time in state 2, exhibiting characteristic abnormalities in corticosubcortical and corticocerebellar connectivity. In state 3, a less frequently state observed across the ADHD and TD children, ADHDC was differentiated from ADHDI by significant alterations in FC between bilateral temporal regions and other brain areas in comparison with TD. Across all three states, several strategic brain regions, mostly bilateral, exhibited significant alterations in both static functional connectivity (sFC) and dFC in the ADHD groups compared to TD, including inferior, middle and superior temporal gyri, middle frontal gyri, insula, anterior cingulum cortex, precuneus, calcarine, fusiform, superior motor area, and cerebellum. Our results show distributed abnormalities in sFC and dFC between different large-scale RSNs including cortical and subcortical regions in both ADHD subtypes compared to TD. Our findings show that the dynamic changes in brain FC can better explain the underlying pathophysiology of ADHD such as deficits in visual cognition, attention, memory and emotion processing, and cognitive and motor control.
Structural and biochemical insight into the mechanism of dual CpG site binding and methylation by the DNMT3A DNA methyltransferase

Abstract DNMT3A/3L heterotetramers contain two active centers binding CpG sites at 12 bp distance, however their interaction with DNA not containing this feature is unclear. Using randomized substrates, we observed preferential co-methylation of CpG sites with 6, 9 and 12 bp spacing by DNMT3A and DNMT3A/3L. Co-methylation was favored by AT bases between the 12 bp spaced CpG sites consistent with their increased bending flexibility. SFM analyses of DNMT3A/3L complexes bound to CpG sites with 12 bp spacing revealed either single heterotetramers inducing 40° DNA bending as observed in the X-ray structure, or two heterotetramers bound side-by-side to the DNA yielding 80° bending. SFM data of DNMT3A/3L bound to CpG sites spaced by 6 and 9 bp revealed binding of two heterotetramers and 100° DNA bending. Modeling showed that for 6 bp distance between CpG sites, two DNMT3A/3L heterotetramers could bind side-by-side on the DNA similarly as for 12 bp distance, but with each CpG bound by a different heterotetramer. For 9 bp spacing our model invokes a tetramer swap of the bound DNA. These additional DNA interaction modes explain how DNMT3A and DNMT3A/3L overcome their structural preference for CpG sites with 12 bp spacing during the methylation of natural DNA.
Elevated plasma levels of NT-proBNP in ambulatory patients with peripheral arterial disease

N-terminal pro B-type natriuretic peptide (NT-proBNP), a cardiac disease biomarker, has been demonstrated to be a strong independent predictor of cardiovascular events in patients without heart failure. Patients with peripheral arterial disease (PAD) are at high risk of cardiovascular events and death. In this study, we investigated levels of NT-proBNP in patients with PAD compared to non-PAD controls. A total of 355 patients were recruited from outpatient clinics at a tertiary care hospital network. Plasma NT-proBNP levels were quantified using protein multiplex. There were 279 patients with both clinical and diagnostic features of PAD and 76 control patients without PAD (non-PAD cohort). Compared with non-PAD patients, median (IQR) NT-proBNP levels in PAD patients were significantly higher (225 ng/L (120–363) vs 285 ng/L (188–425), p- value = 0.001, respectively). Regression analysis demonstrated that NT-proBNP remained significantly higher in patients with PAD relative to non-PAD despite adjusting for age, sex, hypercholesterolemia, smoking and hypertension [odds ratio = 1.28 (1.07–1.54), p-value <0.05]. Subgroup analysis showed elevated NT-proBNP levels in patients with PAD regardless of prior history of CHF, CAD, diabetes and hypercholesteremia (p-value <0.05). Finally, spearmen’s correlation analysis demonstrated a negative correlation between NT-proBNP and ABI (ρ = -0.242; p-value < 0.001). In conclusion, our data shows that patients with PAD in an ambulatory care setting have elevated levels of NT-proBNP compared to non-PAD patients in the absence of cardiac symptoms.