Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of human endometrial glandular epithelium in vitro and in vivo.

Endometrial glands provide histiotrophic support for the developing conceptus prior to the onset of the haemochorial placenta. Several lines of evidence suggest that glands also play an important role in endometrial receptivity, decidualization of the stromal compartment, and in maternal immune tolerance during pregnancy. However, glandular epithelial cells isolated during the luteal phase become acutely arrested in vitro, precluding in-depth analysis of this cellular compartment of the human endometrium. In this thesis, I tested various approaches to overcome the senescence-associated cell cycle block of primary human endometrial epithelial cells (HEECs). I demonstrate that conditional reprogramming of HEECs, using conditioned medium of irradiated fibroblast and a Rho kinase inhibitor (Y-27632), partially reverses the senescent phenotype and enables expansion of primary HEEC cultures. However, the responsiveness of reprogrammed HEECs to embryo-derived signals and hormonal cues remained highly variable. To overcome this hurdle, I used a novel 3D culture system that enabled formation of glandular structures from clonal HEECs seeded in Matrigel and cultured in modified adult stem cell medium. Treatment of glandular organoids with cyclic AMP and steroid hormones induced the expression of PAEP, a glandular differentiation marker. Organoid-forming efficiency experiments revealed that missed miscarriage, characterized by early-onset foetal growth retardation, is associated with glandular progenitor cell deficiency. To validate this observation, I used laser capture microdissection coupled to RNA-sequencing to compare mid-luteal glandular gene expression between missed miscarriage cases and control subjects. Gene ontology analysis of differentially expressed genes revealed that miscarriage may be caused by bioenergetics defects in the glands, exemplified by altered expression of mitochondrial-related genes. Taken together, the ability to grow and differentiate endometrial glands from isolated clonal HEECs provides a powerful new tool to study the mechanisms underpinning reproductive failure.
Scientific opinion of Flavouring Group Evaluation 205 Revision 1 (FGE.205Rev1): consideration of genotoxicity data on representatives for 13 ,unsaturated aliphatic ketones with terminal double bonds and precursors from chemical subgroup 1.2.2 of FGE.19

The EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF Panel) was requested to consider in the Flavouring Group Evaluation 205 (FGE.205), the additional data on genotoxicity submitted by the Industry on two representative substances, oct-1-en-3-one [FL-no: 07.081] and pent-1-en-3-one [FL-no: 07.102], from subgroup 1.2. 2 of FGE.19. The Panel concluded that both substances were weakly genotoxic in bacteria with pent-1-en-3-one being the most potent (previously available data). In these assays, the representative substances were highly cytotoxic with a steep toxicity curve and with a very narrow concentration range resulting in mutagenicity. Both substances were also tested in mammalian cells for gene mutations at the hprt locus and for structural and numerical chromosomal aberrations in the micronucleus assay. Also in mammalian cells, the test substances were highly cytotoxic. The Panel considered that the positive effects in the bacterial mutagenicity assays of the two representative substances cannot be overruled by the one negative and one equivocal gene mutation test in mammalian cells. Therefore, the Panel recommended to test the most potent of the representative substances, pent-1-en-3-one, in an in vivo Comet assay on the first site of contact (e.g. the stomach or duodenum) and on the liver. The Industry has now submitted new data, a combined micronucleus and Comet assay for pent-1-en-3-one, with scoring in the liver and duodenum, and an Ames test and a Comet assay with scoring in the liver for oct-1-en-3-one. Based on these new data, the Panel concluded that the concern for a genotoxic potential could be ruled out for the two representative substances and accordingly for the remaining 11 substances in FGE.205Rev1. The 13 substances in FGE.205Rev1 can therefore be evaluated via the Procedure. © 2016 European Food Safety Authority. EFSA Journal published by John Wiley and Sons Ltd on behalf of European Food Safety Authority.
A study on the phytochemical analysis and corrosion inhibition on mild steelby Annona Muricata .L leaves extract in 1 N hydrochloric acid

Extract of Annona muricata L. leaves was investigated as a corrosion inhibitor of mild steel in 1 N HCl using conventional weight loss, electrochemical polarization, electrochemical impedance spectroscopy, and scanning electron microscopic studies. The weight loss results showed that the extract of Annona muricata L leaves is an excellent corrosion inhibitor, electrochemical polarization data revealed the mixed mode of inhibition, and the results of electrochemical impedance spectroscopy have shown that the change in the impedance parameters, charge transfer resistance, and double layer capacitance, with the change in concentration of the extract, is due to the adsorption of active molecules leading to the formation of a protective layer on the surface of mild steel. Scanning electron microscope studies provided the confirmatory evidence of an improved surface condition, due to the adsorption, for the corrosion protection
MICROPROPOGATION AND ANALYSIS OF PHYTOCHEMICAL PROFILE OF TISSUE CULTURE GROWN PLANTAGO OVATA FORSK.

Objectives : Plantago ovata is an important medicinal plant of Himalayan region greatly used in herbal dugs manufacturing. The plant is multipurpose and strictly present in the Himalaya. Plantago has many medicinal properties such as antioxidant, anti-inflammatory and hematopoiesis effects and protects the liver and is used for the treatment of cancer. The plant being medicinal possesses complex phytochemicals. The investigation of various Plantago organ (leaves, stem etc) revealed their high potential to produce a wide array of bioactive secondary metabolites. In present study the a new method of micropropagation through tissue culture was developed for Plantago so as to meet the future demand of plant. Futher a morphological and physiochemical comparison of tissue culture grown plant was done with in vivo grown plants. Methods: Plantago ovata was grown in -vitro through tissue culture technique using MS media and in-vivo in the nursery area of Shoolini University. In vitro culture of Plantago ovata forsk. were managed to restrict the ecological factors and to control the culture conditions. Experimental culture parameter including germination and phytochemical constituents of Plantago ovata in vivo and in vitro conditions were observed. Results: The result revealed changes in the concentration of phytochemical constituent’s in tissue culture grown Plantago . Phytochemicals constituents (carbohydrate, tannin, chlorophyll, saponin) was reduced in tissue culture grown plant where as some phytochemicals (phenol, alkaloid, flavanoid, protein, phytosterol) increased in tissue culture grown plant than in vivo plant. A reduction in morphological trait was found in tissue cultured plant. Conclusion: The developed tissue culture method for the micropropagation of Plantago ovata can be used as milestone to meet the industrial need in near future. Keywords : Plantago , Tissue Culture Technique, germination, phytochemicals.
Deep Learning personalised, closed-loop Brain-Computer Interfaces for multi-way classiﬁcation

—Brain-Computer Interfaces are communication systems that use brain signals as commands to a device. Despite being the only means by which severely paralysed people can interact with the world most effort is focused on improving and testing algorithms ofﬂine, not worrying about their validation in real life conditions. The Cybathlon’s BCI-race offers a unique opportunity to apply theory in real life conditions and ﬁlls the gap. We present here a Neural Network architecture for the 4-way classiﬁcation paradigm of the BCI-race able to run in real-time. The procedure to ﬁnd the architecture and best combination of mental commands best suiting this architecture for personalised used are also described. Using spectral power features and one layer convolutional plus one fully connected layer network we achieve a performance similar to that in literature for 4-way classiﬁcation and prove that following our method we can obtain similar accuracies online and ofﬂine closing this well-known gap in BCI performances.
Effects of subsoiling on maize yield and water-use efficiency in a semiarid area

Abstract A 3-year field research was conducted to investigate the effect of four subsoiling methods on maize yield and water use efficiency. These four subsoiling methods included bulk subsoiling in autumn (BS), annual ridge subsoiling in autumn, annual furrow subsoiling in early summer, and interannual alternate zone subsoiling (AS), which were compared with rotary tillage in spring. The results suggested that the bulk density of the soil was alternately changed by AS, but AS had limited effect on the average water storage in the soil. Overall, soil tilled by BS and AS maintained a high water-holding capacity, which could stabilize the yield of maize under varying rainfall across different years. Maize under the AS subsoiling method maintained a relatively higher production capacity and water-use efficiency compared with those under the other three types of subsoiling methods.
Fruit production and quality evaluation of four litchi cultivars (Litchi chinensis Sonn.) grown in Mediterranean climate.

the affirmed cultivar harvested in many tropical areas. ‘Tai So’ in addition to its reasonable yielding, physicochemical traits, contents of protein and vitamin, had the highest sensory appeal. ‘Kwai Mai’ showed interesting percentage of flesh, TSS/TA and flesh mineral composition. ‘Brewster’ and ‘Wai Chee’ revealed interesting ground colour, vit. B 2 and vit. C contents. On the other hand, ‘Brewster’ and ‘Tai So’ presented alternate bearing causing to lose a market year. Conclusion – These results suggest the possibility to produce litchi in Mediterranean climate with promising yields and an elevated standard of fruit quality. (Vit. B ) content was higher in ‘Tai So’ and ‘Kwai Mai’, followed by ‘Brewster’ and ‘Wai Chee’. These results are in line with other studies conducted on litchi fruit in tropical areas and, in the case of Vit. B 1 , show very high contents (Menzel, 2002).
Environmental Attitudes and Tourist Satisfaction in Overloaded Natural Protected Areas

Positive attitudes toward the natural environment are supposedly associated with higher levels of satisfaction in the visitation of natural protected areas. However, this relationship has not yet been thoroughly investigated. A reasonable alternative hypothesis can be considered in the case of overloaded natural protected areas (i.e., with high levels of visitation and infrastructure). Too many people and abundant facilities in natural areas could eliminate the effect of the pro-environmental attitude on satisfaction. Environmentalist visitors might feel the area has been spoilt by crowds and overconstruction, or simply they might not find the close contact with nature they were looking for. These concurrent hypotheses were tested using data from 434 interviews conducted with long-haul tourists visiting the Iguaçu National Park in Brazil. The results showed that, despite the crowds and large-scale infrastructure in the park, environmentalists are still more satisfied than nonenvironmentalists with the visitation experience. Managerial implications are discussed.
Mastitis pathogens and their resistance against antimicrobial agents in herds of dairy cows situated in marginal parts of Slovakia

Marginal regions are relatively large part of the area Slovakia which in terms of the economy breeding ruminants can efficiently produce animal commodities only occasionally. Geographic, social and economic stability of these regions is strongly influenced by breeding of ruminants with market milk production. Mastitis is a disease complex that assumes highest clinical and economic significance in milk animals particularly medium to high yielding dairy cattle, usually in and around periparturient period. The objective of this study was to evaluate the effectiveness of different antibiotics against mastitis causing microorganisms during first month of lactation in two herds of 230 and 310 dairy cows situated in marginal parts of Slovakia. Milk samples from quarters were cultured and identified bacteria were subjected to antimicrobial susceptibility test by disc diffusion method to a large number of antibiotics. The prevalence of mastitis in the monitored herds of dairy cows was 26.1% to 17.6%, respectively. A total of 1663 milk samples from udder quarters were investigated, 446 (21.3%) samples were positive. No pathogens were isolated from 1663 (78.4%) milk samples. From all tested bacteria Staphylococcus spp. and Streptococcus spp. which were isolated from subclinical and clinical mastitis, were found amoxicillin + clavulanat and tetradelta to be most effective drug followed by ceftiofur and rifaximin. The significant difference was confirmed between the Staph. aureus and coagulase-negative staphylococci (CoNS) isolates with respect to their susceptibility to the various antibiotics. Antibiotic susceptibility tests should be done to determine the effectiveness of drug that can be used for successful treatment of diseases. Proper isolation and identification of the causative organism play significant role in prevention and control of the diseases.
School Social Work in Vietnam: Development and Capacity Building through International Collaboration

International collaborations and exchanges have been a part of the social work profession since its beginning. The internationalization of social work is firmly established with the presence of multiple international social work organizations. In the specialization of school social work, there are opportunities for exchange through an international school social work conference held every two to three years. It was at this conference that the authors met to initiate a collaboration in support of the development of school social work in Vietnam. This paper discusses the collaborative efforts and project funded by a Fulbright Specialist grant to support the development of school social work in Vietnam. The professional development seminars provided opportunities for a deeper understanding of the role of the school social worker in psychosocial assessment, needs assessment, addressing issues such as bullying, mental health challenges, suicidality, multi-tiered systems of support, and the evidence based process. As a still evolving specialization, school social work would benefit from continued international collaborations and building opportunities for intellectual and professional exchanges.
A Two-Stage Mutation Stochastic Model of Carcinogenesis Driven by a Two Level Random Environment

In this paper, we present a two-stage stochastic model of carcinogenesis in a two level random environment. The random environment switches between two levels, say, 1 and 2 alternately. When the environment is in level 1, a normal cell either divides into two normal cells or dies; and an intermediate cell divides into two intermediate cells or dies. When the environment is in level 2, a normal cell either divides into two intermediate cells or divides into one normal cell and one intermediate cell or divides into two normal cells or dies; and an intermediate cell either divides into two malignant cells or divides into one intermediate cell and one malignant cell or divides into two intermediate cells or dies. It is assumed that, once a malignant cell is produced, it generates a malignant tumor with probability 1. We obtain the mean numbers of normal, intermediate and malignant cells. KeywordsAge-dependent two-stage stochastic model, Normal cell, Intermediate cell, Malignant cell, Random environment.
Cognitive flexibility mediates the association between early life stress and habitual behavior

Accumulating evidence suggests that exposure to high levels of early life stress (ELS) may lead to a lasting shift between goal-directed and habitual behavioral tendencies. Cognitive flexibility has been shown to be impaired following early life stress and represents a protective factor for the formation of rigid maladaptive behavior, however, whether cognitive flexibility mediates their association is not clear. Against this background we employed a mediation approach in a sample of n = 560 young healthy Chinese to determine whether cognitive flexibility mediates the association between ELS and habitual behavioral tendencies as assessed by the Creature of Habits Scale (COHS). We present and validate a Chinese version of the COHS (COHS-C) and replicate the two factor solution of the original version. Higher ELS exposure was associated with higher habitual behavioral tendencies and lower cognitive flexibility. Importantly, the association between ELS and habitual behavior was fully mediated by cognitive flexibility, suggesting that ELS-associated deficient cognitive flexibility promotes habitual behavioral tendencies in everyday life. Early intervention approaches that aim at promoting cognitive flexibility may increase resilience for dysregulated habit formation following ELS in adulthood.
Investigation of the Endothelial Response of Super Elastic Braided Stent: An Experimental Evaluation

Amac: Bu calismada yeni super elastik ciplak stentlerin vaskuler biyoyararlaniminin deneysel olarak arastirilmasi ve stent implante edilen damarlarin kan akisindaki orta donem sonuclarinin ultrasonografik olarak izlenmesi amaclandi. Yontem: Calisma adimlarinin belirlenmesinden sonra stentler iki koyun ilyak arterlerine implante edildi. Ameliyat oncesi ve sonrasi kan akisi ve lokal bulgular kaydedildi. Denekler anormal vucut degisiklikleri acisindan periyodik olarak takip edildi ve stent implante edilen damarlar Doppler Ultrasonografi ile kontrol edildi. Denekler 6. ayda sakrifiye edildi ve iliak arterler histopatolojik olarak incelendi. Bulgular: Takip sirasinda stent implante edilmis damarlarda herhangi bir okluzyon paterni gozlenmedi. Histopatolojik analiz, stent yuzeyinde yeterli yeniden endotelizasyona ragmen, her iki stent bolgesinin de belirgin neointimal hiperplazi olmadigini ortaya koydu. Makroskopik veya mikroskopik bulgularda okluzif veya stenotik patern saptanmadi. Sonuc: Sonuclarimiz, yeni super elastik ciplak stentlerin, orta donem bulgularina gore yuksek aciklik oranlari ile iyi vaskuler biyoyararliliga sahip oldugunu gostermistir.
Changes in Some Enzymes Activities of Amaranthus viridis L. Inoculated with Telfairia Mosaic Virus (TeMV)

Introduction: Investigations were carried out to assess changes in some enzymes activities of Amaranthus viridis (A. viridis) inoculated with Telfairia mosaic virus (TeMV). Methodology: Symptoms of Telfairia mosaic virus (TeMV) are characterized by severe mosaic and leaf malformation. Healthy and inoculated leaves of A. viridis were assayed for changes in peroxidase (POD), polyphenol oxidase (PPO), glucose-6-phosphate dehydrogenase (G6PdH) and 6-phosphogluconate dehydrogenase (6PGdH) activities spectrophotometrically at wavelengths appropriate for each enzyme for a period of 120 days after inoculation (DAI). Activities of enzymes in TeMV inoculated and healthy plants of A. viridis were measured in µg -1 FW and U/L. Results: Results revealed a gradual increase in activities of POD and PPO from 14 - 60 DAI, followed by a decline at 90 DAI and a subsequent increase at 120 DAI in inoculated plants while G6PdH and 6PGdH activities showed gradual increase from 14 – 30 DAI, followed by a decrease from 60 – 90 DAI and subsequent increase at 120 DAI. Activities of POD, PPO, G6PdH and 6PGdH at all periods of growth were significantly (P = 0.05) higher in inoculated plants compared to the healthy. Results also revealed that activities of all enzymes in U/L were significantly higher than activities in µg -1 FW. Fluctuation in all assayed enzymes activities was observed in inoculated samples. Highest percentage increases in enzymes activities in µg -1 FW for POD, PPO, G6PdH and 6PGdH were 54.3%, 45.3%, 55.3% and 44.6% respectively. Corresponding activities in U/L were 81.2%, 86.2%, 91.3% and 87.0%. Conclusion: Activities of all enzymes were found to increase in inoculated leaves due to TeMV infection when compared to healthy leaves. Changes induced by TeMV infection led to increase and fluctuation in all enzymes activities with alterations in physiological processes of A. viridis climaxing to poor growth and reduction in yield.
The Behaviour of Barnacle Cyprids in Relation to Water Movement over a Surface

1. The velocity gradient of the fluid close to the solid boundary is the most appropriate description of the conditions of water flow which affect the attachment of the larvae of sessile forms to solid objects. The nominal speed of the water movement past the object is of importance only in so far as it influences the velocity gradient in the boundary layer. 2. Experiments in glass tubes on cypris larvae of Elminius modestus and Balanus balanoides show that moderate velocity gradients exceeding 500 sec. -1 sweep the cyprids past the surface before they can attach. For large objects exposed to turbulent flow, the critical velocity gradient corresponds very approximately to a flow of 1-2 knots. 3. Attachment under conditions of water flow is accompanied by negative rheotaxy, and can occur equally in the light and in the dark. 4. Maximum attachment occurs at or below velocity gradients just great enough for the cyprid to be able to maintain position by swimming along the surface against the current. 5. Once the cyprid has attached it cannot be pulled off the surface even by gradients greatly in excess of those which prevent attachment. 6. Cyprids can migrate in all directions when exposed to moderate gradients, but they do so only with difficulty when the velocity gradient is high, particularly if the water is flowing in the same direction as that in which the cyprids are walking. The direction in which the cyprid migrates is altered only momentarily by changes in the direction of the current, the animal actively resisting the redistribution of forces acting on it. 7. No direct evidence is given in this paper on the influence of water currents on fixation, but a critical comparison with other published work suggests that fixation can occur in places where the velocity gradients are greater than those which limit attachment, provided the cypris is able to migrate there after attachment. 8. Moderate velocity gradients have little effect on the orientation at metamorphosis. Individuals tend to settle with the anterior end pointing downstream rather than in any other position. This orientation is the opposite from that which would be expected if the cyprid were passively orientated by the water current, but is likely to make subsequent feeding more efficient. 9. The ability to attach under conditions of water flow, and the tendency not to attach under stagnant conditions, may have an important influence on the animals' distribution and survival. 10. Solid particles in suspension may profoundly influence the behaviour, hence the results given in this paper may not be relevant to conditions where scouring takes place.