Miracle-dz/UniFAR-FTUs huggingface dataset:
CXCR4 and CXCR7 Inhibition Ameliorates the Formation of Platelet–Neutrophil Complexes and Neutrophil Extracellular Traps through Adora2b Signaling

Peritonitis and peritonitis-associated sepsis are characterized by an increased formation of platelet–neutrophil complexes (PNCs), which contribute to an excessive migration of polymorphonuclear neutrophils (PMN) into the inflamed tissue. An important neutrophilic mechanism to capture and kill invading pathogens is the formation of neutrophil extracellular traps (NETs). Formation of PNCs and NETs are essential to eliminate pathogens, but also lead to aggravated tissue damage. The chemokine receptors CXCR4 and CXCR7 on platelets and PMNs have been shown to play a pivotal role in inflammation. Thereby, CXCR4 and CXCR7 were linked with functional adenosine A2B receptor (Adora2b) signaling. We evaluated the effects of selective CXCR4 and CXCR7 inhibition on PNCs and NETs in zymosan- and fecal-induced sepsis. We determined the formation of PNCs in the blood and, in addition, their infiltration into various organs in wild-type and Adora2b−/− mice by flow cytometry and histological methods. Further, we evaluated NET formation in both mouse lines and the impact of Adora2b signaling on it. We hypothesized that the protective effects of CXCR4 and CXCR7 antagonism on PNC and NET formation are linked with Adora2b signaling. We observed an elevated CXCR4 and CXCR7 expression in circulating platelets and PMNs during acute inflammation. Specific CXCR4 and CXCR7 inhibition reduced PNC formation in the blood, respectively, in the peritoneal, lung, and liver tissue in wild-type mice, while no protective anti-inflammatory effects were observed in Adora2b−/− animals. In vitro, CXCR4 and CXCR7 antagonism dampened PNC and NET formation with human platelets and PMNs, confirming our in vivo data. In conclusion, our study reveals new protective aspects of the pharmacological modulation of CXCR4 and CXCR7 on PNC and NET formation during acute inflammation.
Life Cycle Primary Energy Use in Buildings of High Energy Standards

In this study, we examine the life cycle primary energy use of buildings. Our analysis starts with a conventional timber-frame building. We maintain the architectural design, but alter the thermal properties of the envelope components and include heat recovery of ventilation air to achieve buildings with similar thermal properties as three existing passive house in Sweden. We also vary the building frame material from timber to concrete, and the heat supply system between district heating and electric resistance heating. We then follow the life cycle of the buildings, analyze and compare their life cycle primary energy use, including the production, operation and end-of-life energy uses. The results show that the life cycle primary energy use of a passive house building is substantially lower when it is constructed with a timber instead of a concrete frame and heated with district heating instead of electricity. A passive house with electric heating does not achieve lower primary energy use than a conventional house with district heating. Material production accounts for a relatively large share of the life cycle primary energy use in passive standard buildings, and is proportionally more significant if the heat supply is from district heating. Material choice is thus relatively more important as buildings become more energy efficient and energy-efficient heat supply systems are used.
Potential of Fatty Acid Amide Hydrolase (FAAH), Monoacylglycerol Lipase (MAGL), and Diacylglycerol Lipase (DAGL) Enzymes as Targets for Obesity Treatment: A Narrative Review

The endocannabinoid system (ECS) plays an integral role in maintaining metabolic homeostasis and may affect hunger, caloric intake, and nutrient absorption. Obesity has been associated with higher levels of the endogenous cannabinoid transmitters (endocannabinoids). Therefore, the ECS is an important target in obesity treatment. Modulating the enzymes that synthesize and degrade endocannabinoids, namely fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MAGL), and diacylglycerol lipase (DAGL), may be a promising strategy to treat obesity. This review aims to synthesize all studies investigating pharmacological or genetic manipulation of FAAH, MAGL, or DAGL enzymes in association with obesity-related measures. Pharmacological inhibition or genetic deletion of FAAH tended to promote an obesogenic state in animal models, though the relationships between human FAAH polymorphisms and obesity-related outcomes were heterogeneous, which could be due to FAAH having both pro-appetitive and anti-appetitive substrates. Genetic deletion of Mgll and Dagla as well as pharmacological inhibition of DAGL tended to reduce body weight and improve metabolic state in animal studies, though the effects of Mgll manipulation were tissue-dependent. Monitoring changes in body weight in ongoing clinical trials of FAAH inhibitors may clarify whether FAAH inhibition is a potential therapeutic strategy for treatment obesity. More preclinical work is needed to characterize the role of MAGL and DAGL modulation in obesity-related outcomes.
3D-Printed Miniature Robots with Piezoelectric Actuation for Locomotion and Steering Maneuverability Applications

The miniaturization of robots with locomotion abilities is a challenge of significant technological impact in many applications where large-scale robots have physical or cost restrictions. Access to hostile environments, improving microfabrication processes, or advanced instrumentation are examples of their potential use. Here, we propose a miniature 20 mm long sub-gram robot with piezoelectric actuation whose direction of motion can be controlled. A differential drive approach was implemented in an H-shaped 3D-printed motor platform featuring two plate resonators linked at their center, with built-in legs. The locomotion was driven by the generation of standing waves on each plate by means of piezoelectric patches excited with burst signals. The control of the motion trajectory of the robot, either translation or rotation, was attained by adjusting the parameters of the actuation signals such as the applied voltage, the number of applied cycles, or the driving frequency. The robot demonstrated locomotion in bidirectional straight paths as long as 65 mm at 2 mm/s speed with a voltage amplitude of only 10 V, and forward and backward precise steps as low as 1 µm. The spinning of the robot could be controlled with turns as low as 0.013 deg. and angular speeds as high as 3 deg. /s under the same conditions. The proposed device was able to describe complex trajectories of more than 160 mm, while carrying 70 times its own weight.
The Integration of Human Factors into Discrete Event Simulation and Technology Acceptance in Engineering Design

This action research thesis aimed to: 1) develop and test a viable Discrete Event Simulation and Human Factors Modeling approach for an Ontario based telecommunication company, and 2) identify the factors that affect the uptake and application of the approach in work system design. This approach, which was validated at the Company, incorporated fatigue dose and learning curves in a Discrete Event Simulation model. The barriers to uptake included: Time constraints, lack of technological knowledge and initial cost. The uptake facilitators were: High frequency products produced, clear value added to leadership, defects reduction and the Company being open to new technology. In addition to helping design a manual assembly line with fewer bottlenecks and reduce the human factors risks for the employee, the developed approach showed a 26% correlation with quality defects. Further research is recommended to identify additional human factors and their benefits.
Sterile docking using microwave heating

Peritonitis is the single most limiting factor for patients undergoing continuous ambulatory peritoneal dialysis (CAPD), a treatment associated with end stage renal failure (ESRD). A device which destroys microorganisms that are recognized to be the most problematic in causing peritonitis has been developed. The microwave heating technique showed efficient intraluminal disinfection of CAPD connector pairs. Moist heat has proven to be the most effective technique for disinfecting the inner lumen of commercially available medical connectors. Moist heat using microwave heating has proven to be the most effective and rapid technique. A 10/sup 6/ population reduction of various organisms which are the most prevalent and problematic in causing peritonitis was accomplished with a very short heating cycle. This process is effective due to the rapid heating of the solution contained within the connectors. Since plastic is transparent to microwave heating, the external surface of the plastic connector remains lukewarm during the process. <<ETX>>
On modeling, identification, and control of a heavy-duty electrohydraulic harvester manipulator

Focuses on modeling, parameter estimation, and control for a heavy-duty electrohydraulic manipulator of a harvester machine. The linear-graph method is implemented in deriving mathematical models for the swing, boom and stick subsystems. Actuation dynamics are subsequently integrated with manipulator dynamics to result in a complete machine model. Identification procedures employed in estimating physical parameters are discussed in detail and key parameter results supplied. Model validation studies show good agreement between model predictions and experiments. A Cartesian controller for the motion of the manipulator end-point is described and response results are presented. It is shown that the obtained response is very good for the purposes of this harvester machine, resulting in very small relative tracking errors.
Hilltopping influences spatial dynamics in a patchy population of tiger moths

Dispersal is a key driver of spatial population dynamics. Dispersal behavior may be shaped by many factors, such as mate-finding, the spatial distribution of resources, or wind and currents, yet most models of spatial dynamics assume random dispersal. We examined the spatial dynamics of a day-flying moth species (Arctia virginalis) that forms mating aggregations on hilltops (‘hilltopping’) based on long-term adult and larval population censuses. Using time-series models, we compared spatial population dynamics resulting from empirically-founded hilltop-based connectivity indices, and modeled the interactive effects of temperature, precipitation, and density dependence. Model comparisons supported hilltop-based connectivity metrics over random connectivity, suggesting an effect of hilltopping behavior on dynamics. We also found strong interactive effects of temperature and precipitation on dynamics. Simulations based on fitted time series models showed lower patch occupancy and regional synchrony, and higher colonization and extinction rates when hilltopping was included, with potential implications for the probability of persistence of the patch network. Overall, our results show the potential for dispersal behavior to have important effects on spatial population dynamics and persistence, and we advocate inclusion of such non-random dispersal in metapopulation models.
IL-25 Treatment Improves Metabolic Syndrome in High-Fat Diet and Genetic Models of Obesity

Introduction Endemic obesity is considered the driving force for the dramatic increase in incidence of type 2 diabetes (T2D). There is mounting evidence that chronic, low-grade inflammation driven by Th1/Th17 cells and M1 macrophages, is a critical link between obesity and insulin resistance. IL-25 promotes development of a Th2 immune response and M2 macrophages that counteract the inflammation associated with obesity and T2D. Methods Mice were fed a high-fat diet (HFD) for 16 weeks and then treated with IL-25 or BSA as a control for 21 days. Body weight, blood glucose levels, intraperitoneal glucose tolerance, and gene expression were evaluated in mice treated with BSA or IL-25. Ob/ob mice fed a normal control diet were also treated with BSA or IL-25 and body weight and blood glucose levels were measured. Transepithelial electrical resistance and sodium-linked glucose absorption were determined in muscle-free small intestinal tissue and glucose absorption assessed in vitro in intestinal epithelial and skeletal muscle cell lines. Results Administration of IL-25 to HFD fed mice reversed glucose intolerance, an effect mediated in part by reduction in SGLT-1 activity and Glut2 expression. Importantly, the improved glucose tolerance in HFD mice treated with IL-25 was maintained for several weeks post-treatment indicating long-term changes in glucose metabolism in obese mice. Glucose intolerance was also reversed by IL-25 treatment in genetically obese ob/ob mice without inducing weight loss. In vitro studies demonstrated that glucose absorption was inhibited by IL-25 treatment in the epithelial IPEC-1 cells but increased glucose absorption in the L6 skeletal muscle cells. This supports a direct cell-specific effect of IL-25 on glucose metabolism. Conclusion These results suggest that the IL-25 pathway may be a useful target for the treatment of metabolic syndrome.
Co-Creation of a Multi-Component Health Literacy Intervention Targeting Both Patients with Mild to Severe Chronic Kidney Disease and Health Care Professionals

Limited health literacy (LHL) is common in chronic kidney disease (CKD) patients and frequently associated with worse self-management. Multi-component interventions targeted at patients and healthcare professionals (HCPs) are recommended, but evidence is limited. Therefore, this study aims to determine the objectives and strategies of such an intervention, and to develop, produce and evaluate it. For this purpose, we included CKD patients with LHL (n = 19), HCPs (n = 15), educators (n = 3) and students (n = 4) from general practices, nephrology clinics and universities in an Intervention Mapping (IM) process. The determined intervention objectives especially address the patients’ competences in maintaining self-management in the long term, and communication competences of patients and HCPs. Patients preferred visual strategies and strategies supporting discussion of needs and barriers during consultations to written and digital strategies. Moreover, they preferred an individual approach to group meetings. We produced a four-component intervention, consisting of a visually attractive website and topic-based brochures, consultation cards for patients, and training on LHL for HCPs. Evaluation revealed that the intervention was useful, comprehensible and fitting for patients’ needs. Healthcare organizations need to use visual strategies more in patient education, be careful with digitalization and group meetings, and train HCPs to improve care for patients with LHL. Large-scale research on the effectiveness of similar HL interventions is needed.
Macroscopic response of Ni–Mn–Ga single crystals considering the effects of residual reorientation strain and temperature

The magnetic field–induced and stress-induced response of magnetic shape memory alloy was investigated in the present study. Based on thermodynamic approach, a one-dimensional constitutive model was derived containing demagnetization energy and chemical energy. A cosine function was constructed to predict the variant volume fraction during reorientation process, considering the presence of residual reorientation strain. The effects of temperature on the magnetization behaviour, domain fraction and magnetization angle were discussed. Owing to the presence of residual reorientation strain, the critical threshold fields in hysteresis loop were decreased as a linear function of residual variant volume fraction. With increasing temperature, critical fields for domain and magnetic rotation to saturation were decreased, and critical threshold fields would increase for the stress-induced reorientation. The comparisons between numerical results and experimental results showed that the present model can effectively predict the macroscopic response of magnetic shape memory alloy, considering the effects of temperature and residual reorientation strain.
Three-dimensional, finite element interaction between soil and simple tillage tool

ABSTRACT A non-linear, three-dimensional, finite element model was used to study soil-tool interaction. The model included both effects of soil strength and soil-metal friction behavior. An interface element between the soil and the tool surface was established based upon a hyperbolic model. Laboratory tillage tests were conducted in a soil bin to verify the results of the finite element model. The results showed that the finite element analysis predicted an accurate draft force for a simple tillage tool. The relative prediction error ranged from 0.8% to 10.5%. KEYWORDS. Tillage, Draft force. Finite element model.
Robustness and stability of synchronized chaos: an illustrative model

Synchronization of two chaotic systems is not guaranteed by having only negative conditional or transverse Lyapunov exponents. If there are transversally unstable periodic orbits or fixed points embedded in the chaotic set of synchronized motions, the presence of even very small disturbances from noise or inaccuracies from parameter mismatch can cause synchronization to break down and lead to substantial amplitude excursions from the synchronized state. Using an example of coupled one dimensional chaotic maps we discuss the conditions required for robust synchronization and study a mechanism that is responsible for the failure of negative conditional Lyapunov exponents to determine the conditions for robust synchronization.
Multifaceted production strategies and applications of glucosamine: a comprehensive review

Abstract Glucosamine (GlcN) and its derivatives are in high demand and used in various applications such as food, a precursor for the biochemical synthesis of fuels and chemicals, drug delivery, cosmetics, and supplements. The vast number of applications attributed to GlcN has raised its demand, and there is a growing emphasis on developing production methods that are sustainable and economical. Several: physical, chemical, enzymatic, microbial fermentation, recombinant processing methods, and their combinations have been reported to produce GlcN from chitin and chitosan available from different sources, such as animals, plants, and fungi. In addition, genetic manipulation of certain organisms has significantly improved the quality and yield of GlcN compared to conventional processing methods. This review will summarize the chitin and chitosan-degrading enzymes found in various organisms and the expression systems that are widely used to produce GlcN. Furthermore, new developments and methods, including genetic and metabolic engineering of Escherichia coli and Bacillus subtilis to produce high titers of GlcN and GlcNAc will be reviewed. Moreover, other sources of glucosamine production viz. starch and inorganic ammonia will also be discussed. Finally, the conversion of GlcN to fuels and chemicals using catalytic and biochemical conversion will be discussed.
Correlation between Rheological Fatigue Tests on Bitumen and Various Cracking Tests on Asphalt Mixtures

Accurate characterisation and appropriate binder selection are essential to increase the load-induced cracking resistance of asphalt mixtures at an intermediate temperature. Hence, the primary goal of this study was to correlate the cracking resistance exerted by the binder with the cracking performance of asphalt mixtures. The laboratory-based experimental plan covered various types of laboratory tests specified by various agencies and road authorities to study the correlation of a neat bitumen and five polymer-modified binders with their corresponding asphalt mixtures. The fatigue life of the binders was assessed through a Linear Amplitude Sweep (LAS) test and statistically correlated with various load-induced cracking parameters from the indirect tensile test, semi-circular bending (SCB) test, and four points bending beam test (FPBB) of asphalt mixtures at 25 °C. Binders and mixes were further grouped depending on their polymeric family (i.e., modified with a particular type of polymer) to validate their statistical correlation. The indicator that mostly correlated the binder properties with the asphalt mixture properties is the secant modulus from the SCB test. Fatigue parameters obtained through LAS better explain the asphalt fatigue performance obtained through FPBB; specifically, asphalt tests at high strain levels (e.g., 400 micro strain) better correlate to the LAS fatigue parameter (Nf).
Assessing Mechanisms Underlying the Sharing of Official and Unofficial Information during a Pandemic

The COVID-19 disease outbreak has seen mixed information flows comprising top-down communication from health authorities to the public and citizen-to-citizen communication. This study aimed to identify mechanisms underlying the sharing of official versus unofficial information during the outbreak. Survey findings based on a nationally representative U.S. sample (N = 856) showed that individuals’ predispositions affected their information consumption and affective experiences, leading to distinct types of information-sharing behaviors. While anger toward the U.S. government’s outbreak response was directly associated with unofficial information sharing, anxiety was directly associated with official information sharing. These findings enhance our understanding of the propagation of different kinds of pandemic information and provide implications for public education on information verification based on source authoritativeness.
Numerical–Experimental Approach to Characterize Fracture Properties of Asphalt Mixtures at Low Temperatures

Fracture damage mechanisms are some of the most significant causes of structural failure in asphalt mixtures. Ye t much research is still needed to understand properly the fracture process in such complex materials. The study reported in this paper investigated several experimental testing protocols available in the literature to characterize fracture properties of asphalt mixtures. Two bending tests (i.e., semicircular bending, single-edge notched beam) and one tension test (disk-shaped compact tension) were performed. An integrated approach that combined experimental tests and numerical simulations was applied to characterize fracture properties of a fine aggregate mixture. The experimental tests were simulated with a computational model on the basis of the finite element method, which was incorporated with material viscoelasticity and cohesive zone fracture. Two cohesive zone fracture parameters (i.e., cohesive strength, fracture energy) were determined through a calibration process until a good match between experimental and numerical results was observed. To illustrate the efficiency of the integrated numerical–experimental approach, fracture properties also were determined through a traditional methodology that used globally averaged material displacements far from the actual fracture process zone. The results indicated that different fracture properties at low temperatures might be obtained from simulations of a single test, regardless of the sample geometry or loading configuration. Through further testing and analysis, it is expected that the modeling approach employed in this work can provide meaningful insights into the effects of constituents on an overall mixture's performance, with significant savings in experimental cost and time.