Miracle-dz/UniFAR-FTUs huggingface dataset:
MASS PRODUCTION OF Rhizopus oligosporus SPORES AND THEIR APPLICATION IN TEMPEH FERMENTATION

TEMPEH is a popular Indonesian food made by fermenting soybeans with Rhi· topus. In 1967, we (Hesseltine et al.) reported that tempeh-like products also could be made from wheat, rice, other cereal grains and various combinations of them. Solid, cake-like tempeh has a mild and pleasant flavor when fried in vegetable oil and, therefore, has the potential for use in a variety of high-protein snacks. Tempeh fermentation is characterized by its simplicity and rapidity. The lack of a suitable inoculum, however, could be a hindrance since it is essential that the inoculum be pure and that spores have a high degree of immediate germinability. Without these conditions, the fermentation process is almost inoperable. Traditionally, small pieces of tempeh from a previous fermentation serve as inoculum. The fungus is then propagated mainly by means of fast-growing mycelia. This practice can lead to contamination by undesirable microorganisms, and the inability of mycelia to survive adverse temperatures and dehydration makes mycelia unsuitable for ~ong-term preservation of their viability. In this country both Hesseltine et ai. (1963) and Steinkraus et al. (1960) c;r;'weloped pure culture fermentation of ~empeh. However, preparing agar media: for mass production of spores is expensive and time-consuming. Steinkraus et ;d. (1965) used freeze-dried, 4-day fermerited soybeans as inoculum. Because we experienced failure and uncertainties with a similar preparation, we undertook to develop a tempeh inoculum having a high, viable spore count that would maintain its viability for a long time with minimal attention.
Chronic endometritis in in vitro fertilization failure patients

In spite of the fact that disordered uterine function is considered to have a major impact on the possibilities to conceive through IVF, conventional infertility investigations, including ultrasound and Hysterosalpingography (HSG) may miss subtle intrauterine lesions. After hysteroscopy maneuver before performing IVF, the incidence of undiagnosed intrauterine lesions has been demonstrated to be at the range of 11 45% [1]. One of these abnormalities is Chronic Endometritis (CE) that is often asymptomatic or can be accompanied by nonspecifi c symptoms. The use of histopathology in detection of plasma cells in the endometrial curettage is essential for the diagnosis of CE [2].
International HRM in the context of uncertainty and crisis: a systematic review of literature (2000–2018)

Abstract This study provides an overview of research on international HRM practices in the context of uncertainty and crisis. With a pool of 72 research articles drawn from 32 journals and published between the years 2000 and 2018, this paper aims to systematically review three distinct research streams within the uncertainty and crisis literature and their effects on international HRM. These three distinct research streams are 1) economic crisis, 2) natural disasters and 3) political uncertainty. In the context of constant economic crisis, we propose further research to explore and integrate high-performance HRM practices and talent management into socially responsible HRM practices. In a natural disaster context, we propose looking into emergency management frameworks within HRM. Finally, in a politically uncertain context, we propose that the development of expatriate assignments and their needs be further investigated. A key contribution of our research is its focus on context and responsiveness in an ever-changing business setting. The importance of boundary conditions and their effect on the implementation of HRM have been overlooked and understudied. A focus on these aspects will provide a nuanced and more realistic understanding of how international HRM unfolds. Relatedly, we consolidate existing research regarding international HRM practices in an uncertainty and crisis context, for our review found it to be scattered and fragmented among disciplines. By doing so, we review existing gaps in the literature and provide a detailed map for future research.
Clinical Risk Factors for Mortality Among Critically Ill Mexican Patients With COVID-19

Little literature exists about critically ill patients with coronavirus disease 2019 (COVID-19) from Latin America. Here, we aimed to describe the clinical characteristics and mortality risk factors in mechanically ventilated COVID-19 patients from Mexico. For this purpose, we recruited 67 consecutive mechanically ventilated COVID-19 patients which were grouped according to their clinical outcome (survival vs. death). Clinical risk factors for mortality were identified by machine-learning and logistic regression models. The median age of participants was 42 years and 65% were men. The most common comorbidity observed was obesity (49.2%). Fever was the most frequent symptom of illness (88%), followed by dyspnea (84%). Multilobe ground-glass opacities were observed in 76% of patients by thoracic computed tomography (CT) scan. Fifty-two percent of study participants were ventilated in prone position, and 59% required cardiovascular support with norepinephrine. Furthermore, 49% of participants were coinfected with a second pathogen. Two-thirds of COVID-19 patients developed acute kidney injury (AKIN). The mortality of our cohort was 44.7%. AKIN, uric acid, lactate dehydrogenase (LDH), and a longitudinal increase in the ventilatory ratio were associated with mortality. Baseline PaO2/FiO2 values and a longitudinal recovery of lymphocytes were protective factors against mortality. Our study provides reference data about the clinical phenotype and risk factors for mortality in mechanically ventilated Mexican patients with COVID-19.
Cervical Cancer Epidemiology, Prevention and Screening among Women Living with HIV in the Americas region: A Systematic Review Protocol

Background: Women living with HIV (WLHIV) are more prone to persistent infection with high-risk Human Papillomavirus (HR-HPV) and development of cervical pre-cancer and cancer. Health systems should integrate HPV and HIV services considering particularities of WLHIV when offering comprehensive cervical cancer services. Objectives: To synthesize existing knowledge on cervical cancer epidemiology, as well as access to screening and prevention among women living with HIV in the Americas Region. Methods: Five PICO questions will orient a systematic review of published literature in Medline and LILACS; supplemented with cross-referencing and search of grey literature in Trip and GoogleScholar. The protocol will follow PRISMA-P standards. Two reviewers will screen titles and abstracts independently; disagreements will be solved by consensus. Verification of eligibility based on full-text reading will be carried out. Studies finally included will be assessed for risk of bias based on María Caicedo Martínez, Camila Ordóñez Reyes, Ginna Fernández Deaza, et al. | Universitas Medica | V. 61 | No. 4 | Octubre-Diciembre | 2020 | 2 standard tools by type of study. Data extraction tables will be built for every PICO question and reported in a structured format. A meta-analysis will be performed depending upon the results. Discussion: This systematic review anticipates finding valuable information for policy makers and public health actors as it aims to provide new data on a special population from the Americas region.
On the application of supported bilayers as receptive layers for biosensors with electrical detection

The application of impedance analysis to biosensor devices based on supported lipid/protein membranes is explored. Lipid membranes with a high degree of surface coverage (≃99.5%) have been prepared by two methods. Firstly, very stable high-resistance bilayers are formed by deposition of a covalently bound monolayer of mercaptoalkyls on gold-covered substrates and subsequent transfer of a lipid monolayer via monolayer transfer. Secondly, the preparation of planar membranes by fusion of vesicles onto charged surfaces is demonstrated
Nomogram for Prediction of fatal outcome in Patients with Severe COVID-19: A Multicenter Study

Background & Aims: To develop an effective model of predicting fatalOutcome in the severe coronavirus disease 2019 (COVID-19) patients.Methods: Between February 20, 2020 and April 4, 2020, consecutive COVID-19 patients from three designated hospitals were enrolled in this study. Independent high- risk factors associated with death were analyzed using Cox proportional hazard model. A prognostic nomogram was constructed to predict the survival of severe COVID-19 patients.Results: There were 124 severe patients in the training cohort, and there were 71 and 76 severe patients in the two independent validation cohorts, respectively. Multivariate Cox analysis indicated that age ≥ 70 years (HR 1.184, 95% CI 1.061-1.321), Panting(breathing rate ≥ 30/min) (HR 3.300, 95% CI 2.509-6.286), lymphocyte count < 1.0 × 109/L (HR 2.283, 95% CI 1.779-3.267), and IL-6 >10pg/mL (HR 3.029, 95% CI 1.567-7.116) were independent high-risk factors associated with fatal outcome. We developed the nomogram for identifying survival of severe COVID-19 patients in the training cohort (AUC 0.900, [95% CI 0.841-0.960], sensitivity 95.5%, specificity 77.5%); in validation cohort 1 (AUC 0.811, [95% CI 0.763-0.961], sensitivity 77.3%, specificity 73.5); in validation cohort 2 (AUC 0.862, [95% CI 0.698-0.924], sensitivity 92.9%, specificity 64.5%). The calibration curve for probability of death indicated a good consistence between prediction by the nomogram and the actual observation. The prognosis of severe COVID-19 patients with high levels of interleukin-6 (IL-6) receiving tocilizumab was better than that of those patients without tocilizumab both in the training and validation cohorts, but without difference (p = 0.105 for training cohort, p = 0.133 for validation cohort 1, and p = 0.210 for validation cohort 2). Conclusions: This nomogram could help clinicians to identify severe patients who have high risk of death, and to develop more appropriate treatment strategies to reduce the mortality of severe patients. Tocilizumab may improve the prognosis of severe COVID-19 patients with high levels of IL-6.
Nondestructive detection of low temperature induced stress on postharvest quality of kápia type sweet pepper

Application of cold storage temperatures below optimum induces a high risk and threat of chilling injury (CI) in the case of sensitive commodities. Sweet pepper belongs to this group of vegetables, so our main objective was to investigate and monitor the effect of non-optimal temperatures (2.5 and 5 °C) induced stress (chilling injury) on kápia type sweet pepper (Capsicum annuum L.) during its postharvest storage by nondestructive quality measuring methods. Fresh, semi-matured (reddish-green colored) samples of ‘Kapitány F1’ cultivar were stored at 2.5, 5 and 10 °C for 7 d followed by 7 d shelf-life. Nondestructive texture measurements were carried out by a purpose built tabletop acoustic stiffness device. Surface color and chlorophyll content related quality indices were evaluated by a chroma meter, a DA-meter® and a chlorophyll fluorescence imaging system. High resolution digital pictures were captured and analyzed for possible CI defects by means of surface color values (normalized RGB, hue and saturation). According to our results, the evaluated quality indices (DA-index®, acoustic stiffness coefficient, surface color parameters; F0, Fm, Fv and Fv/Fm chlorophyll fluorescence parameters) clearly represented the temperature dependent quality changes during low temperature storage, subsequently followed by ambient shelf-life. Samples stored under and at 5 °C showed the chilling temperature stressed symptoms of delayed and partly retarded postharvest ripening, even under simulated shelf-life conditions, but without the onset and manifestation of the characteristic visible symptoms of chilling injury. This may raise doubts and suggest possible future research areas regarding the role of non-optimal cold storage temperatures induced stress, the effect of chilling injury contributing factors and consequences.
Examining the morphometric features of bulbus oculi in Van cats by using computed tomography and magnetic resonance imaging

Accepted date: 10.12. 2020 Abstract: This study was conducted to obtain the morphometric and volumetric measurements of bulbus oculi of Van cats, growing around the city of Van in Turkey and named after here, by using computed tomography (CT) and magnetic resonance imaging (MRI), and to reveal the biometric differences of these measurement values between the sexes. A total of 16 adult Van cats including 8 females and 8 males were used in the study. The animals were anesthetized with the combination of xylazine and ketamine. The anesthetized animals were scanned by using CT and MRI devices and their images were obtained. Then, the morphometric and volumetric measurements of bulbus oculi were calculated from these images using the software (Syngo CT Software) in the workstation and their statistical analysis was performed. Upon the examination of the morphometric and volumetric analysis results, it was determined that while W (bodyweight) and ACL (Left Anterior Chamber) values were higher in male cats, DVLL (Dorsoventral length of the left lens) value was higher in female cats. These differences between the sexes were statistically significant (P<0.05). The volumetric measurement values of bulbus oculi and lens were determined to be averagely 4.60 ± 0.27 cm 3 and 0.67 ± 0.09 cm 3 , respectively. In conclusion, the statistical differences of biometric values of bulbus oculi between male and female Van cats were determined by using CT and MRI. It is thought that the present study would contribute to the ophthalmological applications and the students dorsoventral uzunluğu) değerinin ise dişi kedilerde daha yüksek olduğu tespit edildi. Cinsiyetler arasındaki bu farkların istatistik olarak önemli düzeyde olduğu gözlendi (P<0,05). Bulbus oculi ve lens’e ait volümetrik ölçüm değerleri sırasıyla ortalama 4,60 ± 0,27 cm 3 ve 0,67 ± 0,09 cm 3 olarak belirlendi. Sonuç olarak, Van kedilerinde bulbus oculi’nin biyometrik değerlerinin istatistiksel olarak erkek ve dişiler arasındaki farklılıkları BT ve MRG kullanılarak tespit edildi. Sunulan çalışmanın bu kedilerle ilgili oftalmatolojik uygulamalara ve anatomi eğitimi alan öğrencilere katkı sağlayacağı düşünülmektedir. Anahtar sözcükler: Bilgisayarlı tomografi, bulbus oculi, manyetik rezonans görüntüleme,
Exploring Inflammatory Status in Febrile Seizures Associated with Urinary Tract Infections: A Two-Step Cluster Approach

Background: Urinary tract infections (UTIs) are considered common facilitating factors, along with other infections, in triggering febrile seizures (FS). The main purpose of our study was to identify specific inflammatory patterns of UTI cases from other infections in a specific cluster, using a combination of inflammatory biomarkers to differentiate UTIs from other bacterial diseases triggering FS. Method: This prospective study included a number of 136 patients with 197 distinct FS events, from patients hospitalized in the Pediatric Clinical Hospital Sibiu, among which 10.2% were diagnosed with UTIs. Results: In one-third of the patients with UTIs (20 cases), the symptoms were limited to fever and FS. Using two-step cluster analysis, a distinct UTI inflammatory pattern has emerged: highest platelet values (PLT), median value 331 × 103/mm3 and intermediate C-reactive protein (CRP), median value 15 mg/dL, platelet distribution width (PDW), median value 9.65%, platelet-large cell ratio (P-LCR), median value 14.45%, mean platelet volume (MPV), median value 8.60 fL and neutrophil-to-lymphocyte values (NLR), median value 3.64. Furthermore, higher PDW (median value 12.25%), P-LCR (median value 28.55%), MPV (median value 10.40 fL), CRP (median value 74.00 mg/dL) and NLR values (median value 4.11) were associated mainly (85.7%) with bacterial lower respiratory infections. UTIs were highly unlikely in these patients with significantly increased CRP values and normal values of platelet indices. Conclusions: Considering the nonspecific clinical picture of UTIs at an early age, to optimize the management of FS, a fast diagnosis of UTI is mandatory. The analysis of the inflammatory biomarker clusters (rather than individual parameters) correlated with urine leukocyte and nitrite stick evaluation for specific age groups could help in identifying even oligosymptomatic UTIs patients. The study limitation (20 UTI cases) recommends future multicentric trials on larger datasets to validate the model.
BIOTECHNOLOGICAL VALORIZATION OF AROMATIC PLANTS IN MOROCCO: IMPACT OF EXTRACTION METHODS ON THE YIELD AND CHEMICAL COMPOSITION OF Origanum compactum BENTH. OF TAOUNATE (NORTHERN MOROCCO)

Origanum compactum benth. Native to the Mediterranean basin (Oregano) is a perennial plant, with pubescent stems covered with long hairs; oval-ovate stem leaves its essential oils used worldwide for its aromatic and medicinal virtues, it is considered among the classic products of Morocco in the international market and an important source of Income for the local population of Taounate (Northern Morocco). However, the exploitation of this resource remains primitive and the managers of the valorization units miss scientific information and indicators to understand how the yield and chemical composition of oregano varies according to the extraction material. In order to contribute to the biotechnological valorization of oregano, microwave-assisted hydrodistillation (MAH) and clevenger-assisted hydro distillation (CH) were compared and evaluated for the extraction time, extraction yield, chemical composition and quality of the essential oil. Essential oil extraction of Origanum compactum benth. with MAH and CH are similar in terms of extraction time (60 min), for yield (5.83±0.25 versus 4.47±0.21%), the effect of extraction by MAH remains positive also for the majority compounds of the essential oil, Tymol (79.01 versus 73%), Carvacrol (15.08 versus 12.66%) and Ethyl linolenate (0.91 versus 0.33%).
Experimental Study of NO Removal by Gas Reburning and Selective Noncatalytic Reduction using Ammonia in a Lab-Scale Reactor

An experimental investigation has been performed to study the efficiency of NO reduction by three technological approaches: reburning by methane, selective noncatalytic reduction (SNCR) by ammonia, and a hybrid approach by coupling reburning and SNCR. Experiments were performed on a lab-scale reactor equipped with a McKenna flat flame burner able to generate the flue gas with well-known features. The desired levels of initial NO were achieved by seeding the flame with known amounts of nitric oxide. Experiments were performed throughout the temperature range of interest, i.e., from 973 to 1213 K, to investigate the effects of main working parameters on the performance of the NO reduction process. As a result, very high efficiencies of NO reduction have been obtained using, respectively, methane (up to 90%) and ammonia (up to 75%) as a reducing agent. When these two processes are coupled in a reburn/SNCR hybrid technique, higher efficiencies in NO reduction have been obtained by a comparison to the classica.
Pulmonary Involvement in Patients with Systemic Sclerosis

Background: Systemic sclerosis is a multisystem autoimmune disease of unknown etiology. The pulmonary system is involved in Systemic sclerosis and causes a significant increase in morbidity and mortality. This study aimed to assess the extent of pulmonary system involvement in patient with systemic sclerosis. Methods: This study was a case control study, done on thirty patients with systemic sclerosis and twenty age and sex matched as a control group . All patients included in the study were subjected to the following: Full history, evaluation of skin involvement using modified Rodnan skin thickness score. Investigations including: Pulmonary function test, radiological examination (plain chest X-ray and high-resolution computed tomograghy. Results: predominance of females among patients. 53.33% had limited scleroderma. Modified Rodnen Score ranged from 12 to 35 with mean of 21.03 ± 7.04. Regarding skin changes of systemic sclerosis patients, skin thickness (60%), hyper or hypopigmentation (100%), ischemic ulcers (30%) and subcutaneous calcinosis (20%). A higher frequency of ILD was observed among SSc patients included in this study. The most common patterns in the HRCT in the current study were ground-glass appearance, followed by, honeycombing. Pulmonary hypertension occurred in 30% patients There were highly statistically significant differences (p < 0.001) between cases and the control group regarding PFTs and the severity of restrictive pattern. Conclusion: Pulmonary manifestations are very common in SSc in the form of ILD and PH. PFTs are cornerstone in the evaluation of dyspnea and for detection of pulmonary involvement in SSc patients.
Recent Developments in Diabetic Retinal Neurodegeneration: A Literature Review

Neurodegeneration plays a significant role in the complex pathology of diabetic retinopathy. Evidence suggests the onset of neurodegeneration occurs early on in the disease, and so a greater understanding of the process is essential for prompt detection and targeted therapies. Neurodegeneration is a common pathway of assorted processes, including activation of inflammatory pathways, reduction of neuroprotective factors, DNA damage, and apoptosis. Oxidative stress and formation of advanced glycation end products amplify these processes and are elevated in the setting of hyperglycemia, hyperlipidemia, and glucose variability. These key pathophysiologic mechanisms are discussed, as well as diagnostic modalities and novel therapeutic avenues, with an emphasis on recent discoveries. The aim of this article is to highlight the crucial role of neurodegeneration in diabetic retinopathy and to review the molecular basis for this neuronal dysfunction, its diagnostic features, and the progress currently made in relevant therapeutic interventions.
Climate impacts associated with reduced diet diversity in children across nineteen countries

It is widely anticipated that climate change will negatively affect both food security and diet diversity. Diet diversity is especially critical for children as it correlates with macro and micronutrient intake important for child development. Despite these anticipated links, little empirical evidence has demonstrated a relationship between diet diversity and climate change, especially across large datasets spanning multiple global regions and with more recent climate data. Here we use survey data from 19 countries and more than 107 000 children, coupled with 30 years of precipitation and temperature data, to explore the relationship of climate to child diet diversity while controlling for other agroecological, geographic, and socioeconomic factors. We find that higher long-term temperatures are associated with decreases in overall child diet diversity, while higher rainfall in the previous year, compared to the long-term average rainfall, is associated with greater diet diversity. Examining six regions (Asia, Central America, North Africa, South America, Southeast Africa, and West Africa) individually, we find that five have significant reductions in diet diversity associated with higher temperatures while three have significant increases in diet diversity associated with higher precipitation. In West Africa, increasing rainfall appears to counterbalance the effect of rising temperature impacts on diet diversity. In some regions, the statistical effect of climate on diet diversity is comparable to, or greater than, other common development efforts including those focused on education, improved water and toilets, and poverty reduction. These results suggest that warming temperatures and increasing rainfall variability could have profound short- and long-term impacts on child diet diversity, potentially undermining widespread development interventions aimed at improving food security.