Miracle-dz/UniFAR-FTUs huggingface dataset:
Solid Plasmonic Substrates for Breast Cancer Detection by Means of SERS Analysis of Blood Plasma

Surface enhanced Raman spectroscopy (SERS) represents a promising technique in providing specific molecular information that could have a major impact in biomedical applications, such as early cancer detection. SERS requires the presence of a suitable plasmonic substrate that can generate enhanced and reproducible diagnostic relevant spectra. In this paper, we propose a new approach for the synthesis of such a substrate, by using concentrated silver nanoparticles purified using the Tangential Flow Filtration method. The capacity of our substrates to generate reproducible and enhanced Raman signals, in a manner that can allow cancer detection by means of Multivariate Analysis (MVA) of Surface Enhanced Raman (SER) spectra, has been tested on blood plasma samples collected from 35 healthy donors and 29 breast cancer patients. All the spectra were analyzed by a combined Principal Component-Linear Discriminant Analysis. Our results facilitated the discrimination between healthy donors and breast cancer patients with 90% sensitivity, 89% specificity and 89% accuracy. This is a direct consequence of substrates’ ability to generate diagnostic relevant spectral information by performing SERS measurements on pristine blood plasma samples. Our results suggest that this type of solid substrate could be employed for the detection of other types of cancer or other diseases by means of MVA-SERS procedure.
Reciprocal relationship between multifidus and psoas at L4-L5 level in women with low back pain

Abstract Background Low back pain (LBP) may originate from different sources such as intervertebral disc degeneration (IVDD), end-plate and paraspinal muscle changes. Our aim is to explore the relevance of paraspinal muscles’ fat-infiltration in women with LBP and its association with IVDD and Modic changes. Methods Consecutive female patients presenting with chronic LBP to the outpatient clinics were included. Patients were evaluated in terms of IVDD, vertebral end-plate changes, and fatty infiltration in the paraspinal muscles at all lumbar levels on lumbar spine magnetic resonance imaging (MRI). Visual Analogue Scale (VAS) scores were recorded using our prospectively collected database. Results Patients with higher VAS scores were significantly more likely to have more fatty infiltration in the multifidus and less fatty infiltration in the psoas at L4-L5 level when compared to those with lower VAS scores (69.1 vs. 31.8%, p = 0.003). To predict LBP, fatty infiltration in the multifidus and psoas had odds ratio (OR) of 4 ( p = 0.010), and 0.3 ( p = 0.013), respectively; whereas disc degeneration had an OR of 0.5 ( p = 0.028). Conclusion This is the first clinical cross-sectional study suggested that women with chronic low back pain could have less fat-infiltrated psoas to compensate more fat-infiltrated multifidus at L4-L5 disc level.
Antibacterial potential of essential oils of the needles of Pinus halepensis against Staphylococcus aureus and Escherichia coli

Objective: To examine the in vitro antimicrobial activities of essential oil of the needles of Pinus halepensis (P. halepensis). Methods: The antibacterial activity of essential oil of the needles of P. halepensis was determined using the agar well diffusion technique and disc diffusion method against Staphylococcus aureus ATCC 25923, and Escherichia coli ATCC 25922. Results: The diameter of zones of inhibition exhibited by the essential oil was between 6 and 17 mm. The essential oils was compared favorably with gentamycin used as a standard control. The minimum inhibitory concentration determined by the agar well diffusion method was 0.52 mg/ mL for Staphylococcus aureus and 2.15 mg/mL for Escherichia coli. The minimum bactericidal concentration of the oils against the two microorganisms was 4.17 mg/mL. Conclusions: The results obtained from this study reveal that P. halepensis essential oils possess antibacterial activities and can be used as antimicrobial agents in the search for new drugs.
Response of Aerobic Anoxygenic Phototrophic Bacterial Diversity to Environment Conditions in Saline Lakes and Daotang River on the Tibetan Plateau, NW China

Variations of the pufM gene [encoding the M subunit of the photosynthetic reaction center in aerobic anoxygenic phototrophic (AAnP) bacteria] diversity in response to environmental changes were investigated in waters of six aqueous regimes (including Daotang River and five saline/hypersaline lakes on the Tibetan Plateau) representing a full salinity gradient from freshwater to NaCl-saturation. AAnP bacterial community structures responded to salinity change: Gamma-like AAnP community was predominant in freshwater Daotang River (0.01% salinity). AAnP community structure shifted from Loktanella-like sequences of the Alphaproteobacteria in saline Qinghai Lake to Roseobacter-like sequences in hypersaline lakes (Gahai, Xiaochaidan and Charhan). In addition to salinity, other environmental variables (e.g. N and P availability, TOC and/or DOC, and HCO− 3/CO2− 3 ions) were also important in affecting the pufM gene diversity in hypersaline lakes. These data have important implications for our understanding of the response of AAnP bacterial community to environmental variables in high-altitude aquatic ecosystems.
Codium fragile Ameliorates High-Fat Diet-Induced Metabolism by Modulating the Gut Microbiota in Mice

Codium fragile (CF) is a functional seaweed food that has been used for its health effects, including immunostimulatory, anti-inflammatory, anti-obesity and anti-cancer activities, but the effect of CF extracts on obesity via regulation of intestinal microflora is still unknown. This study investigated anti-obesity effects of CF extracts on gut microbiota of diet-induced obese mice. C57BL/6 mice fed a high-fat (HF) diet were given CF extracts intragastrically for 12 weeks. CF extracts significantly decreased animal body weight and the size of adipocytes, while reducing serum levels of cholesterol and glucose. In addition, CF extracts significantly shifted the gut microbiota of mice by increasing the abundance of Bacteroidetes and decreasing the abundance of Verrucomicrobia species, in which the portion of beneficial bacteria (i.e., Ruminococcaceae, Lachnospiraceae and Acetatifactor) were increased. This resulted in shifting predicted intestinal metabolic pathways involved in regulating adipocytes (i.e., mevalonate metabolism), energy harvest (i.e., pyruvate fermentation and glycolysis), appetite (i.e., chorismate biosynthesis) and metabolic disorders (i.e., isoprene biosynthesis, urea metabolism, and peptidoglycan biosynthesis). In conclusion, our study showed that CF extracts ameliorate intestinal metabolism in HF-induced obese mice by modulating the gut microbiota.
Seedling Development of Sugar Maple and Black Maple Irrigated at Various Frequencies

Growth, dry-matter partitioning, and specific mass of lamina of black maple (Acer nigrum Michx.f.) and sugar maple (A. saccharum Marsh.) irrigated at 10-, 26-, and 42-day intervals were compared. Total dry mass, stem length, and surface area of lamina were greater for sugar maple than for black maple for plants irrigated every 10 days. Reducing irrigation frequency curtailed growth of both species, but the reduction was greater for sugar maple than for black maple. The shoot: root ratio was lower for black maple than for sugar maple and was reduced by drought in both species, particularly among plants irrigated every 26 days
Bipentazole (N10): A Low-Energy Molecular Nitrogen Allotrope with High Intrinsic Stability.

In this letter, we report a crystal structure prediction and characterization of a molecular nitrogen allotrope N10 (bipentazole) using state-of-the-art computational methods. To date, in the form of a P21 space group crystal, this allotrope is the most stable predicted form of nitrogen, other than N2, in the pressure range 0-42 GPa. Its metastability at ambient conditions was justified using phonon dispersion and mechanical properties calculations as well as ab initio molecular dynamics simulations. Due to a high intrinsic stability caused by aromaticity, bipentazole may appear to be the first nitrogen allotrope being stable enough for a large-scale synthesis at ambient conditions. The calculations of propulsive characteristics revealed that bipentazole is an excellent "green" energetic material. A potential strategy for the synthesis of this compound is offered and rationalized. The unique electronic structure of bipentazole makes it a strongly electrophilic all-nitrogen reagent, which can exhibit unusual chemistry.
Changes in shape associated with growth in the loliginid squid Photololigo sp.: a morphometric approach

This study examined growth of squid by describing changes in shape and relative size of body structures in juvenile and adult Photololigo sp. Animals change shape during growth because body structures increase in size at different relative rates. These changes are of particular interest because they are generally concomitant with changes in ecology. Length and mass measurements were taken from Photololigo sp. ranging in size from 2.77 to 117.03 mm dorsal mantle length. Small squid ( 50 mm dorsal mantle length) were more elongate and narrow, and the head and eyes were proportionally smaller. As squid reached a dorsal mantle length of 50 mm, the changes in shape with growth become slower and individuals reached a final shape. Small individuals allocated energy predominantly in the arms and tentacles during early stages of growth, while the viscera and head grew at a much slower rat.
Modeling the Effect of Composition on Formation of Aerosolized Nanoemulsion System Encapsulating Docetaxel and Curcumin Using D-Optimal Mixture Experimental Design

The synergistic anticancer effect of docetaxel (DTX) and curcumin (CCM) has emerged as an attractive therapeutic candidate for lung cancer treatment. However, the lack of optimal bioavailability because of high toxicity, low stability, and poor solubility has limited their clinical success. Given this, an aerosolized nanoemulsion system for pulmonary delivery is recommended to mitigate these drawbacks. In this study, DTX- and CCM-loaded nanoemulsions were optimized using the D-optimal mixture experimental design (MED). The effect of nanoemulsion compositions towards two response variables, namely, particle size and aerosol size, was studied. The optimized formulations for both DTX- and CCM-loaded nanoemulsions were determined, and their physicochemical and aerodynamic properties were evaluated as well. The MED models achieved the optimum formulation for DTX- and CCM-loaded nanoemulsions containing a 6.0 wt% mixture of palm kernel oil ester (PKOE) and safflower seed oils (1:1), 2.5 wt% of lecithin, 2.0 wt% mixture of Tween 85 and Span 85 (9:1), and 2.5 wt% of glycerol in the aqueous phase. The actual values of the optimized formulations were in line with the predicted values obtained from the MED, and they exhibited desirable attributes of physicochemical and aerodynamic properties for inhalation therapy. Thus, the optimized formulations have potential use as a drug delivery system for a pulmonary application.
Anthropogenic and natural effects on the coastal lagoons in the southwest of Spain (Doñana National Park)

The Donana peridunal lagoons, located in the southwest of Spain, have been well studied, because their conservation is of great inter- est. Since 1965, they have also been affected by the extraction of underground water for local coastal tourist resorts. A reconstruction of the evolution of this series of coastal lagoons reveals that, along with the anthropogenic effect, there was a natural effect resulting from the reactivation of mobile dune fronts that have blocked and filled the original lagoon complex—in the period 1920 - 1987, the lagoons were reduced by 70.7%. These fronts might have been fed by deposits of marine sand during the climatically driest phases of the Little Ice Age in Andalusia, Spain. Therefore, if the frequency and duration of dry periods increase, as well as droughts as a whole, because of global warming, the desiccation and disappearance of the lagoons could become more widespread, not only at this site in southwestern Europe, but in other Mediterranean coastal ecosystems as well.
Endometrial Cytology During the Different Phases of the Estrous Cycle in Jennies: New Evidences

Simple Summary This study provides new evidences of the physiological changes of endometrial epithelial cells in cycling jennies during the estrus cycle. The number and morphology of endometrial cells significantly change during the estrous cycle. Endometrial epithelial cell exfoliation is significantly more abundant in estrus than diestrus. The morphological pattern of endometrial epithelial cells shows columnar morphology during estrus, becoming more cuboidal during diestrus. Likewise, degenerative changes observed in later diestrus compared with normal morphology of endometrial epithelial cells in estrus are shown. Scarce segmented neutrophils and erythrocytes are a common response during estrus. The cytobrush (CB) technique represents a suitable method for the endometrial evaluation, taking into account cytopathological purposes in cycling jennies. Abstract Since in the mare and other animal species such as bitches and cats, the endometrial cell pattern varies depending on the phase of the estrous cycle, the aim of this study was to describe and quantify the endometrial cytological (EC) findings in cycling jennies. EC of eight nonpregnant jennies by cytobrush (CB) at diestrus (day 1 and day 14) and estrous (day 21) were evaluated. All slides were stained with Wright´s stain and microscopically examined at both 400× and 1000× magnification. Seven high-power fields (400×) were assessed in each smear and the endometrial epithelial cells and neutrophils (PMNs) were counted. Endometrial epithelial cells were classified as intact, distorted or fragmented and, on the basis of the presence of dense groups, in monolayer or single clusters. Cytoplasmic characteristics, such as vacuolation or streaming and size, form, position of nuclear characteristics, including karyorrhexis, were recorded. Background aspect, as clear, proteinaceous, or debris, was also considered. In general, sampling by CB provided a yield of cells and clumped endometrial epithelial cells in many smears, being more abundant in estrus than early and late diestrus. Individual endometrial epithelial cells, during estrous, presented a columnar morphology, ciliated or not ciliated and basal nuclei. During diestrus phase, endometrial epithelial cells presented a more cuboidal ciliated or not ciliated morphology. Moderate amount of proteinacious material and red blood cells (RBC) was also observed. Non variation in the percentage of PMNs during diestrus was obtained, but lower and segmented PMNs in CB smears were shown in estrous. This study provides new insights on the physiological changes of endometrial epithelial cells in cycling jennies during the estrus cycle. The CB technique represents a suitable and adequate method for endometrial evaluation, taking into account cytological and/or cytopathological purposes also in jennies.
Will the COVID-19 pandemic decrease the FatMax?

X Frédéric Dutheil, X Yolande Esquirol, and X Valentin Navel Université Clermont Auvergne, CNRS, LaPSCo, Physiological and Psychosocial Stress, CHU Clermont-Ferrand, University Hospital of Clermont-Ferrand, Preventive and Occupational Medicine, Witty Fit, Clermont-Ferrand, France; Université Paul Sabatier Toulouse 3, INSERM UMR-1027, University Hospital of Toulouse, CHU Toulouse, Occupational and Preventive Medicine, Toulouse, France; and Université Clermont Auvergne, CNRS, INSERM, GReD, Translational Approach to Epithelial Injury and Repair, CHU Clermont-Ferrand, University Hospital of Clermont-Ferrand, Ophthalmology, ClermontFerrand, France
Disturbances prevent stem size‐density distributions in natural forests from following scaling relationships

Enquist and Niklas propose that trees in natural forests have invariant size-density distributions (SDDs) that scale as a )2 power of stem diameter, although early studies described such distributions using negative exponential functions. Using New Zealand and global data sets, we demonstrate that neither type of function accurately describes the SDD over the entire diameter range. Instead, scaling functions provide the best fit to smaller stems, while negative exponential functions provide the best fit to larger stems. We argue that these patterns are consistent with competition shaping the small-stem phase and exogenous disturbance shaping the large-stem phase. Mortality rates, estimated from repeat measurements on 1546 New Zealand plots, fell precipitously with stem size until 18 cm but remained constant after that, consistent with our arguments. Even in the small-stem phase, where SDDs were best described by scaling functions, the scaling exponents were not invariantly )2, but differed significantly from this value in both the global and New Zealand data sets, and varied through time in the New Zealand data set.
Stability, Regulation, and the Determination of Abundance in an Insular Song Sparrow Population

The population dynamics of the Song Sparrow, Melospiza melodia, were studied for 15 yr on Mandarte Island, in southwestern British Columbia, Canada. This population exhibited a high median density (7.8 females/ha) and fluctuated strongly (10—fold or more) from year to year. The population received few successful immigrants, even at low densities, and its dynamics were thus driven by local events. Two strong density—dependent regulating mechanisms were detected. First, reproductive output was strongly depressed at high densities because of an increased rate of nest failure and a decline in mean clutch size. Greater nest failure at high density was due to increased predation on eggs and nestlings. Nest failure increased with the rate of nest parasitism by Brown—headed Cowbirds (Molothrus ater) and was lowest when cowbirds were absent from Mandarte, suggesting that cowbirds either cause or facilitate nest failure. Second, the rate of juvenile recruitment was inversely related to the density of adults, becaus.
Hot Spot TERT Promoter Mutations Are Rare in Sporadic Pancreatic Neuroendocrine Neoplasms and Associated with Telomere Length and Epigenetic Expression Patterns

Cancer cells activate a telomere maintenance mechanism like telomerase in order to proliferate indefinitely. Telomerase can be reactivated by gain-of-function Telomerase Reverse Transcriptase (TERT) promoter mutations (TPMs) that occur in several cancer subtypes with high incidence and association with diagnosis, prognosis and epigenetics. However, such information about TPMs in sporadic pancreatic neuroendocrine neoplasms (pNENs) including tumor (pNET) and carcinoma (pNEC) is less well defined. We have studied two hot spot TPMs and telomere length (TL) in pNEN and compared the results with clinicopathological information and proliferation-associated miRNA/HDAC expression profiles. DNA was isolated from formalin-fixed paraffin-embedded (FFPE) tissue of 58 sporadic pNEN patients. T allele frequency of C250T and C228T TPM was analyzed by pyrosequencing, relative TL as telomeric content by qPCR. In total, five pNEN cases (9%) including four pNETs and one pNEC were identified with TPMs, four cases with exclusive C250T as predominant TPM and one case with both C250T and C228T. T allele frequencies of DNA isolated from adjacent high tumor cell content FFPE tissue varied considerably, which may indicate TPM tumor heterogeneity. Overall and disease-free survival was not associated with TPM versus wild-type pNEN cases. Binary category analyses indicated a marginally significant relationship between TPM status and longer telomeres (p = 0.086), and changes in expression of miR449a (p = 0.157), HDAC4 (p = 0.146) and HDAC9 (p = 0.149). Future studies with larger patient cohorts are needed to assess the true clinical value of these rare mutations in pNEN.
Could SARS‐CoV‐2 affect male fertility?

We performed this systematic review to evaluate the possibility of an impact of SARS‐CoV‐2 infection on male fertility. SARS‐CoV‐2 enters the cells with the help of ACE2; therefore, testicular expression of ACE2 was analysed from transcriptome sequencing studies and our unpublished data. Literature suggested that SARS‐CoV‐1 (2002‐2004 SARS) had a significant adverse impact on testicular architecture, suggesting a high possibility of the impact of SARS‐CoV‐2 as well. Out of two studies on semen samples from COVID‐19 affected patients, one reported the presence of SARS‐CoV‐2 in the semen samples while the other denied it, raising conflict about its presence in the semen samples and the possibility of sexual transmission. Our transcriptome sequencing studies on rat testicular germ cells showed ACE expression in rat testicular germ cells. We also found ACE2 expression in transcriptome sequencing data for human spermatozoa, corroborating its presence in the testicular germ cells. Transcriptome sequencing data from literature search revealed ACE2 expression in the germ, Sertoli and Leydig cells. The presence of ACE2 on almost all testicular cells and the report of a significant impact of previous SARS coronavirus on testes suggest that SARS‐CoV‐2 is highly likely to affect testicular tissue, semen parameters and male fertility.
Respiratory Therapist Intubation Practice in Pediatric ICUs: A Multicenter Registry Study

BACKGROUND: Tracheal intubation by respiratory therapists (RTs) is a well-established practice that has been described primarily in adult and neonatal patients. However, minimal data exist regarding RTs’ intubation performance in pediatric ICUs. The purpose of this study was to describe the current landscape of intubations performed by RTs in pediatric ICUs. METHODS: A multicenter quality improvement database, the National Emergency Airway Registry for Children (NEAR4KIDS) was queried from 2015 to 2018. We performed a retrospective analysis of prospectively collected data on subject demographics, indication for intubation, difficult airway history and feature presence, provider discipline, medications, and device. Intubation outcomes included first-attempt and overall success rates, adverse events, and oxygen desaturation (ie, SpO2 < 80%). Overall intubation success was defined as intubation achieved in ≤ 2 attempts. RESULTS: There were 12,056 initial intubation encounters from 46 ICUs, with 109 (0.9%) first attempts performed by RTs. Nine (20%) ICUs reported at least one intubation encounter by RTs. The number of intubations performed by RTs at individual centers ranged from 1 to 46 (RT participation rate: 0.3% to 19.6%). RTs utilized video laryngoscopy more often than other providers (53.2% for RTs vs 28.1% for others, P < .001). RTs’ first attempt success (RT 60.6% vs other 69.2%, P = .051), overall success (RT 76.2 % vs other 82.4%, P = .09), and oxygen desaturation SpO2 < 80% (RT 16.5% vs other 16.9%, P = .91) were similar to other providers. Adverse events were more commonly reported in intubations by RTs versus by other providers (22.9% vs 13.8%, P = .006). CONCLUSIONS: RTs infrequently intubate in pediatric ICUs, with success rates similar to other providers but higher adverse event rates. RTs were more likely to use video laryngoscopy than other providers. RTs’ intubation participation, success, and adverse event rates varied greatly across pediatric ICUs.