Miracle-dz/UniFAR-FTUs huggingface dataset:
Experimental Transmission and Pathogenesis of Immunodeficiency Syndrome in Cats

We describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as FeLV-FAIDS) which induces fatal acquired immunodeficiency syndrome (AIDS) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen- free (SPF) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunodeficiency syndrome), depending on the age of the cat at time of virus exposure. The pathogenesis of FeLV-FAIDS-induced feline immunodeficiency disease is characterized by: a prodromal period of largely asymptomatic viremia; progressive weight loss, lymphoid hyperplasia associated with viral replication in lymphoid follicles, lymphoid depletion associated with extinction of viral replication in lymphoid follicles, intractable diarrhea associated with necrosis of intestinal crypt epithelium, lymphopenia, suppressed lymphocyte blastogenesis, impaired cutaneous allograft rejection, hypogammaglobulinemia, and opportunistic infections such as bacterial respiratory disease and necrotizing stomatitis. The clinical onset of immunodeficiency syndrome correlates with the replication of a specific FeLV-FAIDS viral variant, detected principally as unintegrated viral DNA, in bone marrow, lymphoid tissues, and intestine. Two of seven cats with chronic immunodeficiency disease that survived greater than 1 year after inoculation developed lymphoma affecting the marrow, intestine, spleen, and mesenteric nodes. Experimentally induced feline immunodeficiency syndrome, therefore, is a rapid and consistent in vivo model for prospective studies of the viral genetic determinants, pathogenesis, prevention, and therapy of retrovirus-induced immunodeficiency disease.
Changing Practice in Continence Assessment

This pilot project was initiated and carried out by members of a medicine of the elderly continence clinical improvement group. Nurses working in one ward in a hospital specialising in the care of older patients were involved in a practice development project in order to change practice in continence assessment. This project utilised multidisciplinary focus groups, educational tools such as workshops and role play, change management strategies and ongoing facilitation. The project was able to react to many challenges faced whilst implementing changes in practice in the clinical area. For example, the continence assessment tool was incorporated into the pre-existing multidisciplinary team sheet rather than being issued as a ‘stand alone’ document. This meant that the components of the continence assessment were more likely to be documented. Success of the project has been determined through eliciting the reflections of the ward-based multidisciplinary team.
CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4- hydroperoxycyclophosphamide-purged bone marrow

Autologous bone marrow transplants (BMTs) can repopulate the hematologic system of patients treated with marrow-ablative chemotherapy and/or radiotherapy. However, treatment of the bone marrow graft to eliminate residual tumor cells prior to reinfusion can delay the return of peripheral blood elements, presumably from damage to or loss of hematopoietic stem cells responsible for hematologic recovery. To develop a model predictive of hematologic recovery, we studied the progenitor cell contents of 4-hydroperoxycyclophosphamide (100 micrograms/mL)-purged bone marrow grafts of 40 consecutive patients undergoing autologous BMT at this center. Granulocyte-macrophage colonies (CFU-GM) were grown from all grafts after treatment with this chemotherapeutic agent, but erythroid (BFU-E) and mixed (CFU-GEMM) colonies were grown from only 44% and 33% of the grafts respectively. The recovery of CFU-GM after purging ranged from 0.07% to 23%. The logarithm of CFU-GM content of the treated grafts was linearly correlated with the time to recovery of peripheral blood leukocytes (r = -0.80), neutrophils (r = -0.79), reticulocytes (r = -0.60), and platelets (r = -0.66). The CFU-GM content of purged autologous bone marrow grafts may reflect the hematopoietic stem cell content of the grafts and thus predict the rate of hematologic recovery in patients undergoing autologous BMT.
CYTOMEGALOVIRUS ANTIGENEMIA DIRECTED PRE-EMPTIVE PROPHYLAXIS WITH ORAL VERSUS I.V. GANCICLOVIR FOR THE PREVENTION OF CYTOMEGALOVIRUS DISEASE IN LIVER TRANSPLANT RECIPIENTS 1

Background. The efficacy of pre-emptively administered oral ganciclovir in preventing cytomegalovirus (CMV) disease has not been documented in liver transplant recipients. We sought to compare the efficacy of pre-emptive oral ganciclovir with that of i.v. ganciclovir for the prevention of CMV disease after liver transplantation, and to determine whether withholding prophylaxis in the absence of CMV antigenemia, reliably identified patients in whom no prophylaxis was necessary. Methods. Surveillance cultures for CMV pp65 antigenemia were performed in all patients at weeks 2, 4, 6, 8, 12, and 16. Patients with CMV antigenemia were randomized into two study groups. The experimental group received oral ganciclovir for 6 weeks (2 g t.i.d. for 2 weeks, then 1 g t.i.d. for 4 weeks), and the control group received i.v. ganciclovir (5 mg/kg q 12 hr) for 7 days. Results. Of 72 consecutive liver transplant recipients studied, CMV antigenemia occurred in 31% (22 of 72). Twenty-two patients with asymptomatic antigenemia were randomized to two study groups. CMV disease (viral syndrome) occurred in 9% (1 of 11) of the patients in the i.v. ganciclovir group and in 0% (0 of 11) of the patients in the oral ganciclovir group. None of the study patients developed tissue invasive CMV disease. The median reduction in antigenemia level with oral ganciclovir was 55% at week 1, and 100% at week 2. Overall, 64% of the patients by week 1, 93% by week 2, and 100% by week 4 had antigenemia levels below the baseline after oral ganciclovir. Of 50 patients without CMV antigenemia, none developed CMV disease. Conclusions. Pre-emptive prophylaxis based on CMV antigenemia can effectively target the patients for CMV prophylaxis; 69% of the patients never received antiviral prophylaxis and did not develop CMV disease. Antiviral therapy instituted upon detection of antigenemia prevented tissue invasive CMV in both ganciclovir groups. Pre-emptively administered oral ganciclovir was effective as prophylaxis for CMV disease after liver transplantation.
Clinical Management of Malasseziosis associated with Hypothyroidism in a Dog

A 10 month old female Labrador retriever dog was presented with history of recurrent skin disease with loss of hair, severe pruritus, dandruff and oily skin. Malasseziosis was diagnosed by examination of stained impression smears from lesions, cutlural isolation and identification. Thyroid function test revealed significantly lower levels of T4 level. Animal was successfuly treated with Ketaconazole and Levothyroxine.
Oral manifestations of parathyroid disorders and its dental management

Parathyroid hormone plays an important role in the metabolism of calcium and phosphorus, so, influence the mineralization of bone and teeth. Parathyroid disorder may lead to hyper or hyposecretion of hormone, which results in various oral manifestations. Common oral manifestations in patients with hyperparathyroidism (HPT) are brown tumor, loss of bone density, soft tissue calcification, and dental abnormalities. In hypoparathyroidism, the dental abnormalities are a delay or cessation of dental growth and development, paresthesia of the tongue or lips and alteration of the facial muscles. Dentist can easily diagnose the parathyroid disorders owing to their particular oral manifestations and radiographic finding. Dental management of patients with HPT involves a higher risk of bone fracture, whereas in hypoparathyroidism the caries control is the main concern. It is the important that the dentist be aware of the risks and difficulties that may arise during the dental management of these patients.
The (Little) Eect that Parents' Labour Emigration has on their Children's School Performance: a Study of Secondary School Students in Oradea (Romania)

 ere are authors that attribute the decline in the academic performance of the o spring le at home to parents’ migration fo r work.  e main argument explains the phenomena through the impairment of th e family structure due to migration. I used survey data from 1811 high school students from Oradea and multi-level regression to show that the bi-variate relationship between GPA and the situation that both parents have migrated recently for work masks the simple fact that both the lower academic performance and migr ation are the products of a lower socio-economic status.  e conclusion is that parents’ migration for work is not an academic risk for students.
Triagem no Serviço de Emergência: associação entre as suas categorias e os desfechos do paciente

OBJETIVOIdentificar a associacao entre variaveis sociodemograficas, clinicas e categorias de triagem com desfechos do protocolo desenvolvido no Hospital Sao Paulo (HSP). METODOEstudo de coorte retrospectivo realizado com pacientes maiores de 18 anos submetidos ao protocolo de triagem em agosto de 2012. Utilizou-se regressao logistica para associar as categorias de risco aos desfechos (p-valor≤0,05). RESULTADOSHomens com idade mais avancada e atendidos pelas especialidades clinicas apresentaram maiores taxas de internacao e obito. Pacientes com alta prioridade apresentaram taxa de internacao e obitos cinco e 10,6 vezes maior, respectivamente (p<0,0001). CONCLUSAOO grupo de maior prioridade associou-se a maiores taxas de internacao e obitos. O protocolo foi capaz de detectar pacientes com condicoes mais urgentes e identificar fatores de risco para internacao hospitalar e obito.
AN EMPIRICAL STUDY ON PERCEIVED SERVICE QUALITY AND PATIENT SATISFACTION WITH SPECIAL REFERENCE TO PUDUCHERRY HOSPITALS

Recent reforms introduced in Government health sector are likely to create a more responsive and efficient care delivery system. At the same time there has been tremendous growth of the private sector in direct care delivery or the services associated with it. The consumers (patients) of health system also become more alert and demanding. The notion of care quality is talked in various forums and providers of hospital care see quality as a very important future agenda. The present study has analyzed the relationship between demographic variables and patient perceived service quality. It also explored the relationship between demographic variables and patient satisfaction. The results concluded that demographic variable such as age, marital status, family type, education, occupation and income has a significant impact on perceived service quality and patient satisfaction. The findings highlight older, married, joint family type, less educated, business and students tended to rate the care provided by the hospital higher (i.e. more favorable care experiences) than younger, more educated and nuclear family patients.
The relationship between the existence of angiographic coronary artery calcification and the severity of coronary artery disease Związek między zwapnieniami tętnic wieńcowych w angiografii a zaawansowaniem choroby wieńcowej

Background: Coronary artery calcification (CAC) is a predictor of coronary artery disease (CAD) and closely related to major adverse cardiac events. The Gensini score is a scoring system to determine the severity and extent of coronary atherosclerosis. Aim: We aimed to investigate the relationship between CAC and both the extent and severity of CAD. Material and methods: Coronary artery calcification was identified by visual examination during fluoroscopic examination. The study population was divided into two groups, according to the presence or absence of CAC. The CAC(+) group consisted of 126 patients (45 women, 81 men), the CAC(-) group 156 patients (58 women, 98 men). Gensini scores were calculated from the results of coronary angiography. Results: We identified CAC more frequently in type 2 diabetes mellitus patients, with no gender differences. Other risk factors for CAD - including age, hypertension, smoking, body mass index, waist circumference, and systolic blood pressure - were not sig - nificantly different between the CAC(+) and CAC(-) groups. Serum levels of triglyceride and very low density lipoprotein (VLDL) cho - lesterol were significantly lower in the CAC(+) group, and diastolic blood pressure levels and type 2 diabetes mellitus (DM) frequen - cy were higher. By logistic regression, an independent relationship was identified between the Gensini score and CAC. Conclusions: Coronary calcification seems to be more prevalent in type 2 DM patients than in the normal population. The Gensi - ni score, that is, the extent and severity of coronary artery disease, appears to be independently related to CAC, an issue that war - rants further investigation to allow for future clinical applications.
Identification of mutations in the mouse gene encoding spp24 and analysis of protein interactions

Title: Identification of mutations in the mouse gene encoding spp24 and analysis of protein interactions Author: Timothy Francis Secreted phosphoprotein 24 (spp24) was first isolated from bovine cortical bone. The mature human protein consists of 182 amino acids and is encoded by a gene designated SPP2. The cDNA sequence of spp24 has been obtained for fifteen species and homology identifies spp24 as a member of the cystatin superfamily of thiol protease inhibitors. The gene is expressed in a variety of tissues including liver, kidney, bone, cerebellum and lymphocytes. The function of spp24 is unknown, however, evidence suggests that it may be a growth hormone responsive protein, involved with calcification and the cytoskeleton, as well as cancer and osteoarthritis. The aim of this project was to identify more information regarding the function of spp24. Four different but complementary approaches were used; screening DNA samples of ENUmutagenised mice to identify mice with mutations in spp24 and screening an ENUmutagenised ES-cell library, using the yeast two-hybrid system to identify proteins that interact with spp24 and assessing the effect of spp24 mutations on these interactions, generating a mouse with non-functional spp24 (a knockout mouse), and expressing and purifying spp24. Using these approaches five mutations that alter the amino acid sequence of spp24 were identified. Fourteen additional proteins that potentially interact with spp24 were identified but the effect of mutations in spp24 on the interaction strength could not be accurately determined. A spp24 targeting vector to generate a knockout mouse was assembled and transfected but no homologous recombinants could be identified, so it was not possible to generate a knockout mouse. Spp24 expression was attempted using a baculovirus insect cell system, an in vitro system and an E. coli system but no spp24 expression was detected with these systems. Future work will involve generating and characterising mice with mutated spp24 and further analysis of the interaction of spp24 and the proteins identified.
ESTRESSE OCUPACIONAL NO SERVIÇO DE ATENDIMENTO MÓVEL DE URGÊNCIA OCCUPATIONAL STRESS IN THE MOBILE EMERGENCY CARE SERVICE ESTRÉS OCUPACIONAL EN SERVICIOS DE ATENCIÓN MÓVIL DE URGENCIA

This study aims to evaluate the levels of job stress among Marilia’s Mobile Emergency Care Service professionals, consisting of an exploratory and descriptive study. Data collection was performed by a subject’s identification formula and the instrument Escala de Estresse No Trabalho, an adaptation for Portuguese of the original questionnaire Job Stress Scale, created in 1988 by Tores Theorell. Data was analyzed using the software EPIINFO version 6.02. The research’s population was composed of 60 individuals from different categories (nurses, nursing technicians, receptionists, physicians and drivers), and the analysis showed that the subjects have, at the same time, high levels of demand (exigencies and psychological pressures exerted by the job), control (the individual’s capacity to use their intellectual abilities to exercise his or her work and have authority to decide how to do it) and social support (quality of the relationships established by the subject with their bosses and coworkers), consisting of a situation in which the professionals actively experience their work, with a low probability of job stress manifestation. These results remarkably differ from the impression sustained by nonprofessional people and even by health professionals of other specialties about the levels of stress related to the work on emergency, showing the necessity of analyzing the health services under a scientific point of view, in order to demystify beliefs and impressions that often do not match reality.
Prevalência e seguimento de exame citopatológico de colo uterino com atipias em células escamosas de origem indeterminada em um hospital universitário brasileiro

Introducao: O diagnostico de ASC-US no INCA (2009) foi de 1,2%. E importante acompanhar a evolucao clinica, pois um pequeno percentual resultara em HSIL ou carcinoma. Objetivo: acompanhamento por tres anos da evolucao da patologia cervical entre mulheres com resultado de ASC-US. Metodo: Coorte retrospectiva de pacientes do ambulatorio de ginecologia/HCPA com ASC-US. Seguimento: CPs, colposcopias e/ou biopsias. Analise: SPSS (dados descritivos e associacoes). Teste Exato de Fischer/Qui-Quadrado (variaveis qualitativas). Test t de Student/ Wilcoxon-Mann-Whitney (quantitativas). IC 95%, p<0,05; aprovacao GPPG/HCPA (07-286). Resultados: em 2005 foram realizados 9.514 exames CPs; destes, 823 tiveram como resultado ASC-US (8,6%). Amostra final de 320 casos. Tempo medio de seguimento de pacientes com ASC-US: 18,4 meses. Media da idade: 41,3 anos. 77,5% tiveram seguimento normal, 20,3% tiveram ASC-US persistente ou LSIL e 2,2% evoluiram para HSIL; 0,9% de carcinoma. Historia de DSTs: 35,9%. A evolucao dos casos com lesao persistente por ASC-US, evolucao para LSIL ou HSIL foi significativamente maior no grupo de pacientes com historia previa de DSTs (31,3% x 17,6% OR 2,14; IC 1,25-3,65; P<0,005), confirmando-as como fator de risco para lesoes cervicais. Discussao e Conclusoes: o percentual de exames com ASC-US encontrado em nosso meio esta de acordo com a literatura. A evolucao favoravel, com remissao total das alteracoes citopatologicas, tambem esta dentro do esperado, assim como o numero de LSIL, HSIL e carcinoma. O câncer cervical e uma das neoplasias malignas com maior possibilidade de prevencao e programas adequados de rastreamento precoce podem ter impacto importante na morbimortalidade da doenca.
Hospitalized Patients Awareness of Their Rights in Saudi Governmental Hospital

Aim: To determine the level of awareness of patients' rights among hospitalized patients. Methods: A questionnaire survey was conducted among patients at a governmental hospital. Results: Most patients did not know about the patients' bill of rights, even though they are spontaneously practicing their rights. Conclusion: In the studied Saudi hospital, there was a lack of patients' awareness regarding the patients' rights.
Long-Term Follow-up of Patients With Invasive Fungal Disease Who Received Adjunctive Therapy With Recombinant Human Macrophage Colony-Stimulating Factor

Mortality of bone marrow transplant (BMT) patients who develop invasive fungal infection is greater than 80%. Long-term follow-up of 46 consecutive BMT patients who received recombinant human macrophage colony-stimulating factor (rhM-CSF) as adjunctive therapy with standard antifungal treatment who were entered into phase 1/11 trials at The Fred Hutchinson Cancer Research Center is reported. rhM-CSF (100 gg/mz to 2,000 pg/m2; Chiron/ Cetus Corporation, Emeryville, CA) was administered from day 0 to 28 after determination of progressive fungal disease. Results of long-term follow-up of fungal infection, relapse, and survival were compared with 58 similar his-
Adherence to a Mediterranean diet and risk of gastric adenocarcinoma within the European Prospective Investigation into Cancer and Nutrition

Background: The Mediterranean dietary pattern is believed to protect against cancer, although evidence from cohort studies that have examined particular cancer sites is limited. Objective: We aimed to explore the association between adherence to a relative Mediterranean diet (rMED) and incident gastric adenocarcinoma (GC) within the European Prospective Investigation into Cancer and Nutrition study. Design: The study included 485,044 subjects (144,577 men) aged 35–70 y from 10 European countries. At recruitment, dietary and lifestyle information was collected. An 18-unit rMED score, incorporating 9 key components of the Mediterranean diet, was used to estimate rMED adherence. The association between rMED and GC with respect to anatomic location (cardia and noncardia) and histologic types (diffuse and intestinal) was investigated. A calibration study in a subsample was used to control for dietary measurement error. Results: After a mean follow-up of 8.9 y, 449 validated incident GC cases were identified and used in the analysis. After stratification by center and age and adjustment for recognized cancer risk factors, high compared with low rMED adherence was associated with a significant reduction in GC risk (hazard ratio: 0.67; 95% CI: 0.47, 0.94). A 1-unit increase in the rMED score was associated with a decreased risk of GC of 5% (95% CI: 0.91, 0.99). There was no evidence of heterogeneity between different anatomic locations or histologic types. The calibrated results showed similar trends (overall hazard ratio for GC: 0.93; 95% CI: 0.89, 0.99). Conclusion: Greater adherence to an rMED is associated with a significant reduction in the risk of incident GC. Am J Clin Nutr 2010;91:381–90.
Perinatal needs of immigrant Hmong women: surveys of women and health care providers.

: The Hill People of Laos in Southeast Asia, who are called the Hmong, are from a primitive culture which has had a written language for only 31 years. By 1980, about 3,000 of them were living in Colorado, one of 9 States to which they had migrated. In an effort to determine whether or not local health care service was accessible and acceptable to child-bearing families, a pilot survey was conducted in the Denver area. The survey consisted of interviews of the Hmong women themselves and questions of area health care providers. The interviews proved to be both difficult and illuminating. They were difficult because of the language barrier, which required exclusive use of interpreters, and because of the diffidence of the women themselves, especially in discussing matters of sex and childbearing. Illumination came from learning Hmong customs and culture and some of the benefits of their version of self-care. It also came from whatever value may lie in applying this knowledge to other immigrant ethnic groups with comparable problems. Responses to questionnaires from the health care providers disclosed that, from their viewpoint, principal Hmong concerns were family planning and nutrition. They also revealed surprisingly few maternal or child deaths among the Hmong. There still exists a need for both cross-sectional and longitudinal studies to document the effect of migration on the Hmong.