Miracle-dz/UniFAR-FTUs huggingface dataset:
NLRP3 inflammasome activation contributes to the cognitive decline after cardiac surgery

Background Perioperative neurocognitive disorders (PND) are a common complication of cardiac surgery in elderly patients. The etiopathogenesis of PND is not clear. Nod-like receptor family pyrin domain containing 3 (NLRP3) inflammasome, a macromolecular protein complex, regulates inflammation by inducing the release of proinflammatory cytokines interleukin (IL)-1β and IL-18. Studies have demonstrated a close link between the NLRP3 inflammasome and central nervous system diseases. Nevertheless, the involvement of NLRP3 inflammasome in the causation of PND occurring after cardiac surgery is unclear. This study aimed to investigate the association of serum NLRP3 level with PND. Methods We performed a retrospective study, enrolled 75 patients undergoing elective cardiac surgery and evaluated their cognitive functions one day before and 7 days after surgery. PND were determined according to the International Study of Postoperative Cognitive Dysfunction studies. Demographics and perioperative parameters were recorded. Perioperative serum NLRP3 protein, IL-1β, and IL-18 levels were monitored. Results The PND incidence in our cohort was 33.33%. NLRP3 protein levels were significantly increased in all patients at each postoperative time-point after general anesthesia and cardiac surgery under cardiopulmonary bypass. Patients showing cognitive dysfunction had higher serum NLRP3 protein, caspase-1, IL-1β, and IL-18 levels immediately after the operation. Variables associated with the incidence of early PND were included in the regression models. After adjusting for confounding variables, high serum NLRP3 protein level at the end of the operation and old age were identified as independent predictors of PND. Conclusions High serum NLRP3 protein level at the completion of cardiac surgery was associated with a higher risk of PND seven days after surgery. Trial registration The study was registered at Clinicaltrials.gov (registration number: NCT04191642).
Trabecular bone score and sclerostin concentrations in patients with primary adrenal insufficiency

Background Patients with primary adrenal insufficiency need lifelong replacement therapy with glucocorticoids and mineralocorticoids, which may influence their bone quality. Aim The aim of the study was to evaluate densitometry parameters, trabecular bone score and sclerostin concentrations in patients with primary adrenal insufficiency in comparison to control group. Materials and methods We included 29 patients (62% females) with diagnose of autoimmune primary adrenal insufficiency (mean age 49.7 ± 11.7 years, mean duration of the disease 13.2± 13.6 years) and 33 healthy subjects (adjusted with age, sex and body mass index). Bone mineral density at the femoral neck, lumbar spine, total body and trabecular bone score were evaluated. Serum sclerostin concentrations were measured. Results There were no significant differences in densitometry parameters (T-score, Z-score, bone mineral density in all locations) as well as in trabecular bone score in patients with adrenal insufficiency in comparison to control group. Mean serum sclerostin concentration was significantly higher in patients with adrenal insufficiency than in control group (44.7 ± 23.5 vs 30.7 ± 10.4 pmol/l, p=0.006). There was a negative correlation between trabecular bone score and the duration of adrenal insufficiency and age, also a negative correlation between femoral neck and total densitometry parameters and 24-hour urine cortisol as a marker of hydrocortisone daily dose in patients with adrenal insufficiency. Conclusions The bone status in patients with primary adrenal insufficiency was not impaired in comparison to control group, while sclerostin concentration was higher. The duration of the disease and higher hydrocortisone doses may affect negatively bone status.
Activation of orexin-2 receptors in the Kӧlliker-Fuse nucleus of anesthetized mice leads to transient slowing of respiratory rate

Orexins are neuropeptides originating from the hypothalamus that serve broad physiological roles, including the regulation of autonomic function, sleep-wake states, arousal and breathing. Lack of orexins may lead to narcolepsy and sleep disordered breathing. Orexinergic hypothalamic neurons send fibers to Kӧlliker-Fuse (KF) neurons that directly project to the rostroventral respiratory group, and phrenic and hypoglossal motor neurons. These connections indicate a potential role of orexin-modulated KF neurons in functionally linking the control of wakefulness/arousal and respiration. In a reduced preparation of juvenile rats Orexin B microinjected into the KF led to a transient increase in respiratory rate and hypoglossal output, however Orexin B modulation of the KF in intact preparations has not been explored. Here, we performed microinjections of the Orexin B mouse peptide and the synthetic Orexin 2 receptor agonist, MDK 5220, in the KF of spontaneously breathing, isoflurane anesthetized wild type mice. Microinjection of Orexin-2 receptor agonists into the KF led to transient slowing of respiratory rate, which was more exaggerated in response to Orexin-B than MDK 5220 injections. Our data suggest that Orexin B signaling in the KF may contribute to arousal-mediated respiratory responses.
Berbamine inhibits RANKL- and M-CSF-mediated osteoclastogenesis and alleviates ovariectomy-induced bone loss

Osteoporosis is a common public health problem characterized by decreased bone mass, increased bone brittleness and damage to the bone microstructure. Excessive bone resorption by osteoclasts is the main target of the currently used drugs or treatment for osteoporosis. Effective antiresorptive drugs without side effects following long-term administration have become a major focus of anti-osteoporotic drugs. In the present study, we investigated the effect of berbamine, a small molecule natural product from Berberis amurensis Rupr, a traditional Chinese medicine, on RANKL-induced osteoclast differentiation in vitro and ovariectomy-induced bone loss in vivo. The results demonstrated that berbamine at a safe and effective dose inhibited osteoclastogenesis and bone resorption function in vitro by suppressing the nuclear factor-κB signaling pathway. In addition, berbamine protected against osteoporosis by inhibiting osteoclastogenesis and bone resorption function without affecting osteogenesis in the ovariectomy mouse model. These findings revealed that berbamine has a protective role against osteoporosis and may represent a novel promising treatment strategy for osteoporosis.
Connecting gut microbiomes and short chain fatty acids with the serotonergic system and behavior in Gallus gallus and other avian species

The chicken gastrointestinal tract has a diverse microbial community. There is increasing evidence for how this gut microbiome affects specific molecular pathways and the overall physiology, nervous system and behavior of the chicken host organism due to a growing number of studies investigating conditions such as host diet, antibiotics, probiotics, and germ-free and germ-reduced models. Systems-level investigations have revealed a network of microbiome-related interactions between the gut and state of health and behavior in chickens and other animals. While some microbial symbionts are crucial for maintaining stability and normal host physiology, there can also be dysbiosis, disruptions to nutrient flow, and other outcomes of dysregulation and disease. Likewise, alteration of the gut microbiome is found for chickens exhibiting differences in feather pecking (FP) behavior and this alteration is suspected to be responsible for behavioral change. In chickens and other organisms, serotonin is a chief neuromodulator that links gut microbes to the host brain as microbes modulate the serotonin secreted by the host’s own intestinal enterochromaffin cells which can stimulate the central nervous system via the vagus nerve. A substantial part of the serotonergic network is conserved across birds and mammals. Broader investigations of multiple species and subsequent cross-comparisons may help to explore general functionality of this ancient system and its increasingly apparent central role in the gut-brain axis of vertebrates. Dysfunctional behavioral phenotypes from the serotonergic system moreover occur in both birds and mammals with, for example, FP in chickens and depression in humans. Recent studies of the intestine as a major site of serotonin synthesis have been identifying routes by which gut microbial metabolites regulate the chicken serotonergic system. This review in particular highlights the influence of gut microbial metabolite short chain fatty acids (SCFAs) on the serotonergic system. The role of SCFAs in physiological and brain disorders may be considerable because of their ability to cross intestinal as well as the blood-brain barriers, leading to influences on the serotonergic system via binding to receptors and epigenetic modulations. Examinations of these mechanisms may translate into a more general understanding of serotonergic system development within chickens and other avians.
A scoping review on the relationship between robotics in educational contexts and e-health

In recent years, due to technological advancement, research has been directed to the development and analysis of resources and tools related to educational robotics with particular attention to the field of special needs and training actions aimed at learners, teachers, professionals, and families. The use of robotics in all levels of education can support the development of logical and computational thinking, interaction, communication, and socialization, and the acquisition of particularly complex work practices, for example, in the medical field. The adoption of successful educational robotics training practices can be a potential tool to support rehabilitation interventions for disabilities and comprehensive training for students or future professionals in healthcare. A scoping review was conducted on the main topics “education” AND “robotics” with three specific focuses on complementary themes in educational research about ER: (1) teaching and computational thinking, (2) training in the health sector, and (3) education and special needs. The authors systematically searched two online databases, Scopus and Web of Science, up to April 2022. A total of 164 articles were evaluated, and 59 articles were analyzed, in a particular way N = 33 related to computational thinking, N = 15 related to e-health, and N = 11 related to special needs. The following four questions guided our research: (1) What are the educational and experimental experiences conducted through robotics in transdisciplinary fields? (2) What tools and resources are most used in such experiments (educational robotics kit, humanoid robots, telepresence robots etc.)? (3) What are the constitutive elements of the experiments and studies involving robotics and health in educational contexts? and (4) What are those explicitly related to students with special needs? In this study, part of the research project “Robotics and E-health: new Challenges for Education” (RECE) activated at the University of Modena and Reggio Emilia. RECE aims to investigate the training, educational, cognitive, and legal processes induced by the increasing diffusion of educational robotics and telemedicine in clinical and surgical contexts.
The [O iii] Profiles of Infrared-selected Active Galactic Nuclei: More Powerful Outflows in the Obscured Population

We explore the kinematics of ionized gas via the [O iii] λ5007 emission lines in active galactic nuclei (AGNs) selected on the basis of their mid-infrared (IR) emission, and split into obscured and unobscured populations based on their optical−IR colors. After correcting for differences in redshift distributions, we provide composite spectra of spectroscopically and photometrically defined obscured/Type 2 and unobscured/Type 1 AGNs from 3500 to 7000 Å. The IR-selected obscured sources contain a mixture of narrow-lined Type 2 AGNs and intermediate sources that have broad Hα emission and significantly narrower Hβ. Using both [O iii] luminosities and AGN luminosities derived from optical−IR spectral energy distribution fitting, we find evidence for enhanced large-scale obscuration in the obscured sources. In matched bins of luminosity we find that the obscured population typically has broader, more blueshifted [O iii] emission than in the unobscured sample, suggestive of more powerful AGN-driven outflows. This trend is not seen in spectroscopically classified samples, and is unlikely to be entirely explained by orientation effects. In addition, outflow velocities increase from small to moderate AGN E(B − V) values, before flattening out (as traced by FWHM) and even decreasing (as traced by blueshift). While difficult to fully interpret in a single physical model, due to both the averaging over populations and the spatially averaged spectra, these results agree with previous findings that simple geometric unification models are insufficient for the IR-selected AGN population, and may fit into an evolutionary model for obscured and unobscured AGNs.
Fractures reduction with osteoporotic treatments in patients over 75-year-old: A systematic review and meta-analysis

Background: Osteoporosis consists in the reduction of bone mineral density and increased risk of fracture. Age is a risk factor for osteoporosis. Although many treatments are available for osteoporosis, there is limited data regarding their efficacy in older people. Objective: To evaluate the efficacy of osteoporosis treatments in patients over 75 years old. Methods: We reviewed all published studies in MEDLINE, Cochrane and EMBASE including patients over 75 years old, treated by osteoporosis drugs, and focused on vertebral fractures or hip fractures. Results: We identified 4,393 records for review; 4,216 were excluded after title/abstract review. After full text review, 19 records were included in the systematic review. Most studies showed a reduction in vertebral fracture with osteoporosis treatments, but non-significant results were observed for hip fractures. Meta-analysis of 10 studies showed that lack of treatment was significantly associated with an increased risk of vertebral fractures at one (OR = 3.67; 95%CI = 2.50–5.38) and 3 years (OR = 2.19; 95%CI = 1.44–3.34), and for hip fractures at one (OR = 2.14; 95%CI = 1.09–4.22) and 3 years (OR = 1.31, 95%CI = 1.12–1.53). Conclusion: A reduction in the risk of vertebral fractures with osteoporosis treatments was observed in most of the studies included and meta-analysis showed that lack of treatment was significantly associated with an increased risk of vertebral fractures. Concerning hip fractures, majority of included studies did not show a significant reduction in the occurrence of hip fractures with osteoporotic treatments, but meta-analysis showed an increased risk of hip fractures without osteoporotic treatment. However, most of the data derived from post hoc and preplanned analyses or observational studies.
Conduction disorders after transcatheter aortic valve implantation: A comparison between SAPIEN 3 and SAPIEN 3 Ultra balloon-expandable valves

Background Conduction disorders (CD) are the most common complications after Transcatheter Aortic Valve Implantation (TAVI). The last generation of Edwards balloon expandable valves, the SAPIEN 3 Ultra (S3U), is provided with an external sealing skirt that aims to further reduce paravalvular leakage (PVL) compared to SAPIEN 3 (S3) and could potentially lead to higher CD rate. We sought to investigate the rate of new-onset CD in patients undergoing TAVI with the S3 or S3U valve. Methods We included 582 consecutive patients undergoing TAVI in a single high-volume Center. Patients with previously implanted pacemaker and Valve in valve procedures were excluded. CD rate was evaluated early after implantation and at discharge. Results No significant difference in the overall CD rate was found between S3 and S3U patients both immediately after the procedure (S3 45.5% vs. S3U 41.8%, p = 0.575) and at discharge (S3 30.4% vs. S3U 35.6%, p = 0.348) with low rate of permanent pacemaker implantation (S3 6.3% vs. S3U 5.5%, p = 0.749). No significant differences were found also in patients with pre-existing atrial fibrillation (S3 8.2% vs. S3U 5%, p = 0.648). A significantly lower rate of PVL was found with S3U compared to S3 (S3 42% vs. S3U 26%, p = 0.007). According to the manufacturer’s guidelines we confirmed that S3U were implanted in a significantly higher position compared to S3 (S3 4.89 ± 1.57 mm vs. S3U 4.47 ± 1.36 mm, p = 0.001). Conclusion No significant difference in the rate of CD, including the need for PPM implantation, was found in patients undergoing TAVI with the S3 compared to S3U. Moreover, S3U significantly reduced the PVL rate.
An analytical approximation scheme to two-point boundary value problems of ordinary differential equations

A new (algebraic) approximation scheme to find global solutions of two-point boundary value problems of ordinary differential equations (ODEs) is presented. The method is applicable for both linear and nonlinear (coupled) ODEs whose solutions are analytic near one of the boundary points. It is based on replacing the original ODEs by a sequence of auxiliary first-order polynomial ODEs with constant coefficients. The coefficients in the auxiliary ODEs are uniquely determined from the local behaviour of the solution in the neighbourhood of one of the boundary points. The problem of obtaining the parameters of the global (connecting) solutions, analytic at one of the boundary points, reduces to find the appropriate zeros of algebraic equations. The power of the method is illustrated by computing the approximate values of the ‘connecting parameters’ for a number of nonlinear ODEs arising in various problems in field theory. We treat in particular the static and rotationally symmetric global vortex, the skyrmion, the Abrikosov–Nielsen–Olesen vortex, as well as the 't Hooft–Polyakov magnetic monopole. The total energy of the skyrmion and of the monopole is also computed by the new method. We also consider some ODEs coming from the exact renormalization group. The ground-state energy level of the anharmonic oscillator is also computed for arbitrary coupling strengths with good precision.
Seismicity modulation due to hydrological loading in a stable continental region: a case study from the Jektvik swarm sequence in Northern Norway

Seismic swarms have been observed for more than 40 years along the coast of Nordland, Northern Norway. However, the detailed spatio-temporal evolution and mechanisms of these swarms have not yet been resolved due to the historically sparse seismic station coverage. An increased number of seismic stations now allows us to study a nearly decade-long sequence of swarms in the Jektvik area during the 2013-2021 time window. Our analysis resolves four major groups of seismic events, each consisting of several spatial clusters, that have distinct spatial and temporal behaviours. Computed focal mechanism solutions are predominantly normal with NNE-SSW strike direction reflecting a near-vertical maximum principal stress and a NW-SE near-horizontal minimum principal stress, which are controlled by local NW-SE extension. We attribute the swarms to fluid-saturated fracture zones that are reactivated due to this local extension. Over the time period, the activity tends to increase between February and May, which coincides with the late winter and beginning of spring time in Norway. We hypothesize that the seismicity is modulated seasonally by hydrological loading from snow accumulation. This transient hydrological load results in elastic deformation that is observed at local GNSS stations. The loading is shown to promote failure in a critically stressed normal faulting system. Once a segment is activated, it can then also trigger neighboring segments via stress transfer. Our new results point to a close link between lithosphere and hydrosphere contributing to the occurrence of seismic swarm activity in northern Norway.
The population dynamics of insect-pest infesting tomato and there relation with different abiotic factor

An experiment was conducted to study the population dynamics of insect-pest infesting tomato on entomology research farm, Chaudhary Singh University (CCSHAU), Hisar during crop season Rabi 2018-19. It was found that tomato was attacked by tomato fruit borer ( Helicoverpa armigera ), aphid ( Myzus persicae ), and leaf miner ( Liriomyza trifolii ). H. armigera incidence reaching its peak population (5.05 larvae/ plant) in 17 th SMW (4 th week of April) and aphid attained highest population of 23.26 aphids per three leaves during12 th SMW (fourth week of March) . While peak population of whitefly was (6.28 whitefly adults per three leaves) reported during third week of April (16 th SMW). Appearance of leaf miner was observed during first week of March (9 th SMW) with 0.63 mines/ 3 leaves and maximum population (5.95 mines/ 3 leaves) was recorded during 15th (SMW) viz. second week of April. However correlation study with abiotic factors also carried out during experiment.
Embedded Barometric Pressure Sensor Unit for Force Myography in Exoskeletons

Exoskeletons and other wearable devices benefit from sensor systems that are based on biofeedback for detecting muscle activity. However, such sensor systems in wearable applications are often impractical due to the need for direct skin contact or due to non-linear signal output, both requiring extensive calibration and post-processing. This paper presents a compact and robust barometer-based pressure sensor unit for detecting surface muscle pressure in an exoskeleton interface shell that is light, thin and does not require direct contact with the skin. It consists of an array of five miniature barometric sensors mounted on a custom embedded printed circuit board enclosed in a silicon dome. Evaluation of this sensor unit in a controlled experimental setup showed high sensitivity and an almost linear response to the normal force applied to the silicon dome. Additionally, a pilot study was conducted with four participants in a wearable application to compare the performance of the proposed sensor with that of electromyography (EMG). The results showed higher robustness to positioning, consistent signal, low variance and inter-subject variability compared to EMG.
Fatty Acid Profiles in Managed Care Green and Kemp’s Ridley Turtles over Time

To understand fatty acid status and the potential impact of sample storage, dried blood spot (DBS) samples were collected from fourteen green turtles and ten Kemp’s ridleys undergoing rehabilitation. Half the DBS samples from each animal species were collected in 2021 and sent for immediate analysis while the other half were collected in 2017 from different animals and stored at −80 °C for 4 years before analysis. The blood fatty acid profiles in both species during rehabilitation differed visually from prior wild turtle data. Essential fatty acids linoleic (18:2w6) and linolenic acid (18:3w3) were higher in green turtles than in Kemp’s ridleys but both were approximately half of the percentage previously found in wild turtles. No difference in arachidonic acid between species in rehabilitation were found but percentages were approximately 10% of wild representatives. Sea turtle diets in rehabilitation management may need to be evaluated to ensure species-specific fatty acid balance. Twenty-eight of the 36 individual fatty acids tested were found and all seven fatty acid groupings were detected. When analyzed by storage time, 11 individual fatty acids and four fatty acid groups differed (p = 0.05). When compared by species, 14 individual fatty acids and three groups differed. Current data suggest DBS samples may be best utilized when analyzed immediately.