Miracle-dz/UniFAR-FTUs huggingface dataset:
A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application

The use of combination therapies for the treatment of a range of conditions is now well established, with the component drugs usually being delivered either as distinct medicaments or combination products that contain physical mixes of the two active ingredients. There is, however, a compelling argument for the development of compartmentalised systems whereby the release, stability and incorporation environment of the different drugs may be tailored. Here we outline the development of polymeric fine fiber systems whereby two drugs used for the treatment of wounds may be separately incorporated. Fibers were delivered using a newly developed handheld electrospinning device that allows treatment at the site of need. Crucially, the delivery system is portable and may be used for the administration of drug-loaded fibers directly into the wound in situ, thereby potentially allowing domiciliary or site-of-trauma administration. The three-layered fiber developed in this study has polyethylene glycol as the outermost layer, serving as a structural support for the inner layers. The inner layers comprised iodine complexed with polyvinylpyrrolidone (PVP) and metronidazole dispersed in polycaprolactone (PCL) as a slow release core. The systems were characterized in terms of structure and architecture using scanning electron microscopy, transmission electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy and diffractometry. As antibacterial creams are still used for managing infected wounds, the performance of our trilayered fiber was studied in comparison with creams containing similar active drugs. Drug release was measured by UV analysis, while antimicrobial efficiency was measured using agar diffusion and suspension methods. It was found that the trilayered systems, averaging 3.16 µm in diameter, released more drug over the study period and were confirmed by the microbacterial studies to be more effective against P. aeruginosa, a bacterium commonly implicated in infected wounds. Overall, the portable system has been shown to be capable of not only incorporating the two drugs in distinct layers but also of delivering adequate amounts of drugs for a more effective antibacterial activity. The portability of the device and its ability to generate distinct layers of multiple active ingredients make it promising for further development for wound healing applications in terms of both practical applicability and antimicrobial efficacy.
Aging But Not Age-Related Hearing Loss Dominates the Decrease of Parvalbumin Immunoreactivity in the Primary Auditory Cortex of Mice

Abstract Alterations in inhibitory circuits of the primary auditory cortex (pAC) have been shown to be an aspect of aging and age-related hearing loss (AHL). Several studies reported a decline in parvalbumin (PV) immunoreactivity in aged rodent pAC of animals displaying AHL and conclude a relationship between reduced sensitivity and declined PV immunoreactivity. However, it remains elusive whether AHL or a general molecular aging is causative for decreased PV immunoreactivity. In this study, we aimed to disentangle the effects of AHL and general aging on PV immunoreactivity patterns in inhibitory interneurons of mouse pAC. We compared young and old animals of a mouse line with AHL (C57BL/6) and a mutant (C57B6.CAST-Cdh23Ahl+) that is not vulnerable to AHL according to their hearing status by measuring auditory brainstem responses (ABRs) and by an immunohistochemical evaluation of the PV immunoreactivity patterns in two dimensions (rostro-caudal and layer) in the pAC. Although AHL could be confirmed by ABR measurements for the C57BL/6 mice, both aged strains showed a similar reduction of PV+ positive interneurons in both, number and density. The pattern of reduction across the rostro-caudal axis and across cortical layers was similar for both aged lines. Our results demonstrate that a reduced PV immunoreactivity is a sign of general, molecular aging and not related to AHL.
Intra-Cystic (In Situ) Mucoepidermoid Carcinoma: A Clinico-Pathological Study of 14 Cases

Aims: To report on the clinico-pathological features of a series of 14 intra-oral mucoepidermoid carcinomas showing exclusive intra-cystic growth. Materials and methods: All mucoepidermoid carcinomas diagnosed in the period 1990–2012 were retrieved; the original histological preparations were reviewed to confirm the diagnosis and from selected cases, showing exclusive intra-cystic neoplastic components, additional sections were cut at three subsequent 200 m intervals and stained with Hematoxylin–Eosin, PAS, Mucicarmine and Alcian Blue, to possibly identify tumor invasion of the adjacent tissues, which could have been overlooked in the original histological preparations. Additionally, pertinent findings collected from the clinical charts and follow-up data were analyzed. Results: We identified 14 intraoral mucoepidermoid carcinomas treated by conservative surgery and with a minimum follow up of five years. The neoplasms were located in the hard palate (nine cases), the soft palate (two), the cheek (two) and the retromolar trigone (one). In all instances, histological examination revealed the presence of a single cystic space, containing clusters of columnar, intermediate, epidermoid, clear and mucous-producing cells, the latter exhibiting distinct intra-cytoplasmic mucin production, as confirmed by PAS, Mucicarmine and Alcian Blue stains. The cysts were entirely circumscribed by fibrous connective tissue, and no solid areas or infiltrating tumor cell clusters were detected. Conservative surgical resection was performed in all cases, and no recurrences or nodal metastases were observed during follow up. Conclusions: Mucoepidermoid carcinomas showing prominent (>20%) intra-cystic proliferation currently are considered low-grade tumors. In addition, we also unveil the possibility that mucoepidermoid carcinomas, at least in their early growth phase, may display an exclusive intra-cystic component and might be considered as in situ carcinomas, unable to infiltrate adjacent tissues and metastasize.
Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study

Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze–thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS‐scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 µg/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p < .05). The addition of 250 µg/ml of the extract exerted a significant positive effect on sperm motility. Moreover, sperm DNA damage and ROS production were significantly reduced at extract concentrations of 250 and 500 µg/ml (p < .05). To the best of our knowledge, the results of this study represent the first demonstration of the possibility of improving sperm parameters and reducing ROS production and DNA damage by supplementing sperm freezing media with H. parva coelomic extract. Our results suggested that H. parva coelomic extract could be useful for improving the fertilising ability of frozen‐thawed human semen.
Evaluation of the Georeferencing Accuracy of a Photogrammetric Model Using a Quadrocopter with Onboard GNSS RTK

Using a GNSS RTK (Global Navigation Satellite System Real Time Kinematic) -equipped unmanned aerial vehicle (UAV) could greatly simplify the construction of highly accurate digital models through SfM (Structure from Motion) photogrammetry, possibly even avoiding the need for ground control points (GCPs). As previous studies on this topic were mostly performed using fixed-wing UAVs, this study aimed to investigate the results achievable by a quadrocopter (DJI Phantom 4 RTK). Three image acquisition flights were performed for two sites of a different character (urban and rural) along with three calculation variants for each flight: georeferencing using ground-surveyed GCPs only, onboard GNSS RTK only, and a combination thereof. The combined and GNSS RTK methods provided the best results (at the expected level of accuracy of 1–2 GSD (Ground Sample Distance)) for both the vertical and horizontal components. The horizontal positioning was also accurate when georeferencing directly based on the onboard GNSS RTK; the vertical component, however, can be (especially where the terrain is difficult for SfM evaluation) burdened with relatively high systematic errors. This problem was caused by the incorrect identification of the interior orientation parameters calculated, as is customary for non-metric cameras, together with bundle adjustment. This problem could be resolved by using a small number of GCPs (at least one) or quality camera pre-calibration.
Neurocognitive Inhibitory Control Ability Performance and Correlations with Biochemical Markers in Obese Women

Inhibitory control, the ability to suppress prepotent responses and resist irrelevant stimuli, is thought to play a critical role in the maintenance of obesity. However, electrophysiological performance related to different inhibitory control processes and their relationship with motor response inhibition and cognitive interference and potential biochemical mechanisms in middle-aged, obese women are as yet unclear. This work thus compared different neurocognitive Go/Nogo and Stroop task performance in healthy sedentary normal-weight and obese women, as well as their correlation with biochemical markers. Twenty-six healthy, sedentary obese women (obese group) and 26 age-matched (21–45 years old) normal-weight women (control group) were the participants, categorized by body mass index and percentage fat, as measured with dual-energy X-ray absorptiometry. They provided a fasting blood sample and performed two cognitive tasks (i.e., Go/Nogo and Stroop tasks) with concomitant electrophysiological recording. The N2 and P3 waveforms of event-related potential (ERP) were recorded. Although the between-group behavioral performance was comparable, the obese group relative to the control group showed significantly longer N2 latency and smaller P3 amplitude in the Stroop task and smaller N2 and P3 amplitudes in the Go/Nogo task. Significant inflammation response indices (e.g., CRP, leptin, adiponectin/leptin ratio) were observed in the obese group. The Nogo P3 amplitude was significantly correlated with the adiponectin/leptin ratio. These findings indicate that healthy obese women still exhibit deviant neurophysiological performance when performing Go/Nogo and Stroop tasks, where the adiponectin/leptin ratio could be one of the influencing factors for the deficit in neural processes of motor response inhibition.
Management of HPV-Related Squamous Cell Carcinoma of the Head and Neck: Pitfalls and Caveat

Head and neck squamous cell carcinomas (HNSCCs) are a very heterogeneous group of malignancies arising from the upper aerodigestive tract. They show different clinical behaviors depending on their origin site and genetics. Several data support the existence of at least two genetically different types of HNSCC, one virus-related and the other alcohol and/or tobacco and oral trauma-related, which show both clinical and biological opposite features. In fact, human papillomavirus (HPV)-related HNSCCs, which are mainly located in the oropharynx, are characterized by better prognosis and response to therapies when compared to HPV-negative HNSCCs. Interestingly, virus-related HNSCC has shown a better response to conservative (nonsurgical) treatments and immunotherapy, opening questions about the possibility to perform a pretherapy assessment which could totally guide the treatment strategy. In this review, we summarize molecular differences and similarities between HPV-positive and HPV-negative HNSCC, highlighting their impact on clinical behavior and on therapeutic strategies.
A UAV-Based Framework for Semi-Automated Thermographic Inspection of Belt Conveyors in the Mining Industry

Frequent and accurate inspections of industrial components and equipment are essential because failures can cause unscheduled downtimes, massive material, and financial losses or even endanger workers. In the mining industry, belt idlers or rollers are examples of such critical components. Although there are many precise laboratory techniques to assess the condition of a roller, companies still have trouble implementing a reliable and scalable procedure to inspect their field assets. This article enumerates and discusses the existing roller inspection techniques and presents a novel approach based on an Unmanned Aerial Vehicle (UAV) integrated with a thermal imaging camera. Our preliminary results indicate that using a signal processing technique, we are able to identify roller failures automatically. We also proposed and implemented a back-end platform that enables field and cloud connectivity with enterprise systems. Finally, we have also cataloged the anomalies detected during the extensive field tests in order to build a structured dataset that will allow for future experimentation.
Hedgehog Inhibitors Suppress Osteoclastogenesis in In Vitro Cultures, and Deletion of Smo in Macrophage/Osteoclast Lineage Prevents Age-Related Bone Loss

The functional role of the Hedgehog (Hh)-signaling pathway has been widely investigated in bone physiology/development. Previous studies have, however, focused primarily on Hh functions in bone formation, while its roles in bone resorption have not been fully elucidated. Here, we found that cyclopamine (smoothened (Smo) inhibitor), GANT-58 (GLI1 inhibitor), or GANT-61 (GLI1/2 inhibitor) significantly inhibited RANKL-induced osteoclast differentiation of bone marrow-derived macrophages. Although the inhibitory effects were exerted by cyclopamine or GANT-61 treatment during 0–48 h (early stage of osteoclast differentiation) or 48–96 h (late stage of osteoclast differentiation) after RANKL stimulation, GANT-58 suppressed osteoclast formation only during the early stage. These results suggest that the Smo-GLI1/2 axis mediates the whole process of osteoclastogenesis and that GLI1 activation is requisite only during early cellular events of osteoclastogenesis. Additionally, macrophage/osteoclast-specific deletion of Smo in mice was found to attenuate the aging phenotype characterized by trabecular low bone mass, suggesting that blockage of the Hh-signaling pathway in the osteoclast lineage plays a protective role against age-related bone loss. Our findings reveal a specific role of the Hh-signaling pathway in bone resorption and highlight that its inhibitors show potential as therapeutic agents that block osteoclast formation in the treatment of senile osteoporosis.
Patient safety attitudes among critical care nurses: A case study in North Cyprus.

INTRODUCTION Patient safety has become a crucial priority in quality healthcare. Adverse events and serious errors involving critically ill patients are common and can be potentially life-threatening. Thus, this study aimed to examine patient safety attitudes among critical care nurses. METHODS This cross-sectional study was conducted in two hospitals in North Cyprus. Eighty nurses working in critical care units participated in the study. Following ethical approval, data were collected between September and October 2018, using the Demographic Characteristics Questionnaire and Safety Attitudes Questionnaire. FINDINGS Nurses' overall scores regarding patient safety attitudes were found to be negative. The highest positive rate was for safety climate, followed by perception of management, teamwork, working conditions, job satisfaction, and stress recognition, respectively. There were significant differences among working conditions, perception of management, and stress recognition based on participants' positions and event reporting. CONCLUSION Our findings indicate safety culture needs to be improved in the hospitals included in the study. Healthcare managers and decision-makers should foster patient safety culture through in-service education, management support, institutional regulations, and updated guidelines.
Factors Affecting the Decline in Incidence of Diabetes in the Diabetes Prevention Program Outcomes Study (DPPOS)

During the first 7 years of the Diabetes Prevention Program Outcomes Study (DPPOS), diabetes incidence rates, when compared with the Diabetes Prevention Program (DPP), decreased in the placebo (−42%) and metformin (−25%), groups compared with the rates in the intensive lifestyle intervention (+31%) group. Participants in the placebo and metformin groups were offered group intensive lifestyle intervention prior to entering the DPPOS. The following two hypotheses were explored to explain the rate differences: “effective intervention” (changes in weight and other factors due to intensive lifestyle intervention) and “exhaustion of susceptible” (changes in mean genetic and diabetes risk scores). No combination of behavioral risk factors (weight, physical activity, diet, smoking, and antidepressant or statin use) explained the lower DPPOS rates of diabetes progression in the placebo and metformin groups, whereas weight gain was the factor associated with higher rates of progression in the intensive lifestyle intervention group. Different patterns in the average genetic risk score over time were consistent with exhaustion of susceptibles. Results were consistent with exhaustion of susceptibles for the change in incidence rates, but not the availability of intensive lifestyle intervention to all persons before the beginning of the DPPOS. Thus, effective intervention did not explain the lower diabetes rates in the DPPOS among subjects in the placebo and metformin groups compared with those in the DPP.
DEV131235 3613..3613

Here, we provide fundamental insights into early human development by single-cell RNA-sequencing of human and mouse preimplantation embryos. We elucidate conserved transcriptional programs along with those that are human specific. Importantly, we validate our RNAsequencing findings at the protein level, which further reveals differences in human and mouse embryo gene expression. For example, we identify several genes exclusively expressed in the human pluripotent epiblast, including the transcription factor KLF17. Key components of the TGF-β signalling pathway, including NODAL, GDF3, TGFBR1/ALK5, LEFTY1, SMAD2, SMAD4 and TDGF1, are also enriched in the human epiblast. Intriguingly, inhibition of TGF-β signalling abrogates NANOG expression in human epiblast cells, consistent with a requirement for this pathway in pluripotency. Although the key trophectoderm factors Id2, Elf5 and Eomes are exclusively localized to this lineage in the mouse, the human orthologues are either absent or expressed in alternative lineages. Importantly, we also identify genes with conserved expression dynamics, including Foxa2/FOXA2, which we show is restricted to the primitive endoderm in both human and mouse embryos. Comparison of the human epiblast to existing embryonic stem cells (hESCs) reveals conservation of pluripotency but also additional pathwaysmore enriched in hESCs. Our analysis highlights significant differences in human preimplantation development compared with mouse and provides a molecular blueprint to understand human embryogenesis and its relationship to stem cells.
The British Women’s Heart and Health Study and meta-analysis

Fraser, Abigail; Harris, Ross; Sattar, Naveed; Ebrahim, Shah; Davey Smith, George; Lawlor, Debbie A; (2009) Alanine aminotransferase, gamma-glutamyltransferase, and incident diabetes: the British Women’s Heart and Health Study and meta-analysis. Diabetes care, 32 (4). pp. 741-750. ISSN 0149-5992 DOI: https://doi.org/10.2337/dc08-1870 Downloaded from: http://researchonline.lshtm. ac.uk/id/eprint/6056/ DOI: https://doi.org/10.2337/dc08-1870
The Emergent Information Commons: Philosophy, Models, and 21st Century Learning Paradigms

ABSTRACT The learning support role of the Information Commons exhibits emergent properties characteristic of organizational learning theory. The literature review highlights four articles from the United States, one from Germany, and one from Japan to illustrate the issues involved. The philosophy of the commons extension across physical, virtual, and cultural domains and the development of the Learning Commons as a collaboration among multiple learning support units, including libraries, are traced from theoretical origins through real-world examples. “Integrative learning” is offered as one example of a 21st century learning paradigm being supported by such collaborations, as evidenced by the development of commons-based e-portfolio systems.
Canada

Abstract Canada allowed the cultivation and processing of industrial hemp in 1998. This decision was seen as a political dream: “win win” for everyone. There was very little opposition to the idea of industrial hemp: the biggest obstacle was government foot-dragging on the issue. Great expectations were raised then. Now 5 years in, many of these expectations have not been met and the cautious have been vindicated. The growth of the hemp sector has been modest and hemp has proven not to be immune to the struggles typical of any new industry.
A review of plant materials used for controlling insect pests of stored products (NRI Bulletin 65)

The use of plant materials for the protection of stored products against insect pests is reviewed. Following an outline of the different methods used for assessing their insecticidal activity, over 120 plants and plant products are listed alphabetically with a brief description of traditional uses (where appropriate), and a summary of the laboratory data currently available; where possible, information on constituents and toxicology is also included. Of these, only a few, all species in the genera Azadirachta, Acarus, Chenopodium, Eucalyptus, Mentha, Ocimum, Piper and Tetradenia, have been sufficiently tested in the laboratory to give an indication of their potential usefulness as stored product protectants. The neem tree, Azadirachta indica, is considered in a separate section as it has been more extensively studied than the other plants; the traditional uses of neem, its constituents, effects, pests and diseases, toxicity, field trials and commercial applications are outlined, and results of laboratory trials are summarized. It is concluded that more rigorous testing is required if the considerable potential for botanical protectants is to be realized . Standardized assessments of residual effectiveness against all stages of the insect's life cycle need to be undertaken, and mammalian toxicity requires more detailed evaluation. More attention may need to be given to the characterization and quantification of chemical constituents, and to variations which may occur in different karyotypes. It may also be necessary to determine the availability of appropriate extraction and application technology if the use of plant protectants is to be assimilated in developing countries.
Gaydar

Abstract Currently, American gay people believe they have a unique ability to pick each other out in a crowd (often termed “gaydar” [“gay” + “”]). This was established through a nationwide Internet-mediated survey (n = 460). To test for the presence of this ability in gay men, the researcher asked self-identified gay and straight male participants to view a series of unfamiliar men on videotape and determine the sexual orientation of each. The higher overall accuracy of gay men demonstrated a trend level difference from their straight cohorts although falling short (primarily due to small sample size) of the p < 0.05 level. A theory for the emergence of this skill (termed “Adaptive Gaydar” by the author) as a unique perceptual ability/coping mechanism unique among gay people is also presented.