Miracle-dz/UniFAR-FTUs huggingface dataset:
A Seat at the Table: Supporting Persons with Severe Dementia in Communicating Their Preferences

ABSTRACT Objective: Persons with severe dementia are less likely to have a role in preference assessment due to communication challenges associated with the disease. Research is limited on how to support preference communication while using existing preference assessment tools (e.g., the Minimum Data Set 3.0). Methods: This study examined the effect of two assessment conditions (standard verbal; visual-and-text supported;) on residents’ (N = 21) social and leisure preference consistency over 1-week and utterance types (acknowledgment, elaboration, off-topic, request for clarification) in response to preference questions. Residents with severe dementia were recruited from four nursing homes (n = 11) and three assisted living facilities (n = 10). As a preliminary measure of provider acceptability and social validity, a sample of 10 naïve judges (University students) listened to the interviews and rated residents’ communication clarity and their confidence with understanding residents’ preferences. Results: Neither assessment condition promoted significantly greater levels of consistency (i.e., the same preference rating at Time 1 and Time 2). Residents expressed significantly fewer requests for clarification in the visual-and-text supported condition. Naïve judges rated residents’ communication positively, with no significant differences between conditions. Conclusions: This study addresses a gap in current research and holds important implications for enhancing care planning participation by residents with severe dementia. Clinical Implications: Residents with severe dementia can successfully participate in activity preference discussions without proxy participation. Residents may comprehend interview questions better when provided in a supported format.
Zebrafish Xenografts Unveil Sensitivity to Olaparib beyond BRCA Status

Poly (ADP-ribose) polymerase (PARP) inhibition in BRCA-mutated cells results in an incapacity to repair DNA damage, leading to cell death caused by synthetic lethality. Within the treatment options for advanced triple negative breast cancer, the PARP inhibitor olaparib is only given to patients with BRCA1/2 mutations. However, these patients may show resistance to this drug and BRCA1/2 wild-type tumors can show a striking sensitivity, making BRCA status a poor biomarker for treatment choice. Aiming to investigate if the zebrafish model can discriminate sensitivities to olaparib, we developed zebrafish xenografts with different BRCA status and measured tumor response to treatment, as well as its impact on angiogenesis and metastasis. When challenged with olaparib, xenografts revealed sensitivity phenotypes independent of BRCA. Moreover, its combination with ionizing radiation increased the cytotoxic effects, showing potential as a combinatorial regimen. In conclusion, we show that the zebrafish xenograft model may be used as a sensitivity profiling platform for olaparib in monotherapy or in combinatorial regimens. Hence, this model presents as a promising option for the future establishment of patient-derived xenografts for personalized medicine approaches beyond BRCA status.
Disruption of insulin receptor substrate-2 impairs growth but not insulin function in rats

Insulin receptor substrate (IRS)-2, along with IRS-1, is a key signaling molecule that mediates the action of insulin and insulin-like growth factor (IGF)-I. The activated insulin and IGF-I receptors phosphorylate IRSs on tyrosine residues, leading to the activation of downstream signaling pathways and the induction of various physiological functions of insulin and IGF-I. Studies using IRS-2 knockout (KO) mice showed that the deletion of IRS-2 causes type 2 diabetes due to peripheral insulin resistance and impaired β-cell function. However, little is known about the roles of IRS-2 in other animal models. Here, we created IRS-2 KO rats to elucidate the physiological functions of IRS-2 in rats. The body weights of IRS-2 KO rats at birth were lower compared with those of their WT littermates. The postnatal growth of both male and female IRS-2 KO rats was also suppressed. Compared with male WT rats, the glucose and insulin tolerance of male IRS-2 KO rats were slightly enhanced, whereas a similar difference was not observed between female WT and IRS-2 KO rats. Besides the modestly increased insulin sensitivity, male IRS-2 KO rats displayed the enhanced insulin-induced activation of the mTOR complex 1 pathway in the liver compared with WT rats. Taken together, these results indicate that in rats, IRS-2 plays important roles in the regulation of growth but is not essential for the glucose-lowering effects of insulin.
Development of a scale for measuring physician attitudes toward interprofessional education and collaboration

ABSTRACT Interest has been increasing in interprofessional education and collaboration (IPEC) within health professional schools over the last two decades. Although a growing body of literature addresses the effects of IPEC on healthcare outcomes, psychometrically sound attitudinal instruments that measure concepts surrounding IPEC are still needed. The primary objective of this study was to develop a scale to measure the attitudes of attending physicians and residents toward IPEC. Based on a literature review, a set of questionnaire items was drafted to address all six domains outlined in a World Health Organization report for interprofessional learning outcomes. These domains are teamwork and collaboration, roles and responsibilities, communication, reflection and learning, the patient, and ethics and attitudes. A total of 379 physicians and trainees completed the questionnaire. A principal axis factoring with orthogonal varimax rotation of 20 items produced a 5-factor solution explaining 60% of the variance. Examination of the items in each factor led to the following labels: ‘teamwork and communication,’ ‘ethics and attitudes,’ ‘roles and responsibilities,’ ‘reflective practice,’ and ‘patient-centered care. ’ A Generalized Linear Model provided initial evidence that the new scale might detect shifts in attitudes related to some of the emergent factors.
Serum Phospholipid Fatty Acids Levels, Anthropometric Variables and Adiposity in Spanish Premenopausal Women

This study investigates the still uncertain association between serum phospholipid fatty acids (PL-FA), and anthropometric and adiposity variables. A cross-sectional study was conducted with 1443 Spanish premenopausal women. Participants answered an epidemiological and a food frequency questionnaire. Anthropometric variables were measured using a bioimpedance scale. Serum PL-FAs levels were determined by gas chromatography–mass spectrometry. The association between body mass index (BMI), weight gain, body fat percentage, visceral fat index, and waist circumference with serum PL-FAs and desaturation indices was evaluated using multivariable linear regression models. BMI was positively associated with the relative concentration of saturated fatty acids (SFAs) (β = 0.94, q-val = 0.001), and with palmitoleic, dihomo-γ-linolenic (DGLA), arachidonic (AA) and α-linolenic acids, and was inversely associated with oleic, gondoic, trans-vaccenic, linoleic and γ-linolenic acids. Total fat percentage was positively associated with DGLA and AA, and inversely with linoleic and γ-linolenic acids. Low relative concentrations of some SFAs and high levels of n-6 PUFAs were associated with greater waist circumference. While the oleic/stearic and AA/DGLA acid ratios were inversely associated with BMI, DGLA/linoleic acid ratio was positively related to almost all variables. In addition to BMI, total fat percentage and waist circumference were also associated with certain individual fatty acids.
Interpolymer Complexes of Eudragit® Copolymers as Novel Carriers for Colon-Specific Drug Delivery

Interpolymer complexes (IPC) based on Eudragit® EPO and Eudragit® S100 were investigated as potential carriers for oral controlled drug delivery to the colon. IPC samples were prepared by mixing copolymer solutions in organic solvents (ethanol, isopropanol:acetone mixture (60:40, % v/v) and tetrahydrofuran). According to the data of elemental analysis, FTIR-spectroscopy, X-ray photoelectron spectroscopy and thermal analysis these IPCs have excess of anionic copolymer (Eudragit® S100) in their structure; they are stabilized by hydrogen and ionic intermacromolecular bonds and do not include free copolymer domains. IPC have pH-independent swelling properties in the media mimicking gastrointestinal tract (GIT) conditions and provide colon-specific delivery of indomethacin in buffer solutions (pH 1.2; 5.8; 6.8; 7.4) and in biorelevant media (fasted state simulated gastric fluid, fasted state simulated intestinal fluid—version 2 and fasted stated simulated colonic fluid).
UTILIZATION OF SUNFLOWER (HELIANTHUS ANNUUS) HEADS AS ROUGHAGE SOURCE IN COMPLETE DIETS

Two complete diets containing (i) 40% ground sunflower heads and (ii) 20% ground flower heads + 20% lucerne hay, were compared with a cpnventional diet containing concentrate mixture and chopped sorghum straw fed separately by feeding to 3 permanently fistulated crossbred bulls in digestion and metabolism experiments using 3 x 3 latin square. Organic matter, NFE (P<0.05), CP and lignin (P<0.01) digestibilities were significantly higher on complete diet 2. Complete diet I recorded higher (P<0.01) CP and lignin digestibilities compared to conventional diet. DCP content was significantly (P<0.01) higher on complete diets compared to conventional diet. All the experimental animals on all the diets met DCP and TDN requirements. The concentration of all N fractions and TVF A were significantly (P<0.01) higher on complete diet 2 and lower on conventional diet. All the N fractions peaked at 2 hr post feeding and declined thereafter. pH values were lowest at 2 hr post feeding while TVFA concentrations peaked at 4 hr post feeding.
Linking ecologists and traditional farmers in the search for sustainable agriculture

For centuries, traditional farmers have developed diverse and locally adapted agricultural systems, managing them with ingenious practices that often result in both community food security and the conservation of agrobiodiversity. This strategy of minimizing risk stabilizes yields, promotes dietary diversity, and maximizes returns using low levels of technology and limited resources. These microcosms of agricultural heritage still cover no less than 10 million ha worldwide, providing cultural and ecological services not only to rural inhabitants, but to mankind generally. These services include the preservation of traditional farming knowledge, local crop and animal varieties, and native forms of sociocultural organization. By studying these systems, ecologists can enhance their knowledge of the dynamics of complex systems, especially the relationship between biodiversity and ecosystem function and practical principles for the design of more sustainable agroecosystems appropriate to small farmers. Novel a.
A Map of the Posidonia oceanica Beds of Marina ?Elbu (Corsica, Mediterranean)

A large-scale map of the Posidonia oceanica seagrass beds as well as other benthic substrata and communities was compiled for a cove of a marine reserve free from pollution and man-made disturbance. An extensive diving survey of the cove and the interpretation of aerial colour photographs were used. The greater part of the cove is occupied by the P. oceanica meadow. Patches of dead “matte” were observed at a depth of between 7 and 14 m. They may be the result of a natural hydrodynamic equilibrium between building and erosion.
Weed Community Emergence Time Affects Accuracy of Predicted Corn Yield Loss by WeedSOFT

Abstract WeedSOFT is a state-of-the-art decision support system for weed management in the north central region of the United States, but its accuracy to predict corn yield loss associated with later-emerging weed communities has not been adequately assessed. We conducted experiments in 2004 and 2005 to compare observed and predicted corn yield related to four establishment times of mixed-species weed communities for validation of competitive index modifier (CIM) values in WeedSOFT. Weed communities were established at VE, V2, V4, and V6 corn (emergence, second-leaf, fourth-leaf, and sixth-leaf stages, respectively), and consisted largely of annual grass and moderately competitive annual broadleaf species. Compared to weed-free corn, yield loss occurred in each of seven site-years for weed communities established at VE corn, but in only one site-year for communities established at V2 corn. No corn yield loss was associated with weed communities established at V4 or V6 corn. For communities established at VE corn, predicted corn yield differed from observed yield in all but one site year, with predicted yield less than observed yield in three site-years, and greater than observed yield in two site-years; however, nonlinear regression analyses of yield data pooled over site-years showed that fitted values were similar between predicted and observed yield. For communities established at V2 and V4 corn, predicted yield was less than observed yield in six and five site-years, respectively. For communities established at V6 corn, predicted yield was less than observed yield in three of six site-years, but was similar to observed yield in three of six site-years. These results indicated that the CIM values in WeedSOFT tended to overestimate the competitiveness of late-emerging weed communities. Corn yield data from a pooled analysis of all site-years were used to generate a revised set of growth stage CIM values, which improved the accuracy of predicted corn yield. These results should improve weed management decisions and reduce the need for herbicide applications to late-emerging weeds. Nomenclature: Corn, Zea mays L.
Action after Adverse Events in Healthcare: An Integrative Literature Review

Adverse events are common in healthcare. Three types of victims of patient-related adverse events can be identified. The first type includes patients and their families, the second type includes healthcare professionals involved in an adverse event and the third type includes healthcare organisations in which an adverse event occurs. The purpose of this integrative review is to synthesise knowledge, theory and evidence regarding action after adverse events, based on literature published in the last ten years (2009–2018). In the studies critically evaluated (n = 25), key themes emerged relating to the first, second and third victim elements. The first victim elements comprise attention to revealing an adverse event, communication after an event, first victim support and complete apology. The second victim elements include second victim support types and services, coping strategies, professional changes after adverse events and learning about adverse event phenomena. The third victim elements consist of organisational action after adverse events, strategy, infrastructure and training and open communication about adverse events. There is a lack of comprehensive models for action after adverse events. This requires understanding of the phenomenon along with ambition to manage adverse events as a whole. When an adverse event is identified and a concern expressed, systematic damage preventing and ameliorating actions should be immediately launched. System-wide development is needed.
Low Doses of Mycotoxin Mixtures below EU Regulatory Limits Can Negatively Affect the Performance of Broiler Chickens: A Longitudinal Study

Several studies have reported a wide range of severe health effects as well as clinical signs, when livestock animals are exposed to high concentration of mycotoxins. However, little is known regarding health effects of mycotoxins at low levels. Thus, a long-term feeding trial (between May 2017 and December 2019) was used to evaluate the effect of low doses of mycotoxin mixtures on performance of broiler chickens fed a naturally contaminated diet. In total, 18 successive broiler performance trials were carried out during the study period, with approximately 2200 one-day-old Ross-308 chicks used for each trial. Feed samples given to birds were collected at the beginning of each trial and analysed for multi-mycotoxins using a validated LC-MS/MS method. Furthermore, parameters including feed intake, body weight and feed efficiency were recorded on a weekly basis. In total, 24 mycotoxins were detected in samples analysed with deoxynivalenol (DON), zearalenone (ZEN), fumonisins (FBs), apicidin, enniatins (ENNs), emodin and beauvericin (BEV), the most prevalent mycotoxins. Furthermore, significantly higher levels (however below EU guidance values) of DON, ZEN, FBs, BEV, ENNs and diacetoxyscirpenol (DAS) were detected in 6 of the 18 performance trials. A strong positive relationship was observed between broilers feed efficiency and DON (R2 = 0.85), FBs (R2 = 0.53), DAS (R2 = 0.86), ZEN (R2 = 0.92), ENNs (R2 = 0.60) and BEV (R2 = 0.73). Moreover, a three-way interaction regression model revealed that mixtures of ZEN, DON and FBs (p = 0.01, R2 = 0.84) and ZEN, DON and DAS (p = 0.001, R2 = 0.91) had a statistically significant interaction effect on the birds’ feed efficiency. As farm animals are often exposed to low doses of mycotoxin mixtures (especially fusarium mycotoxins), a cumulative risk assessment in terms of measuring and mitigating against the economic, welfare and health impacts is needed for this group of compounds.
Application of capillary electrophoresis to the study of proteolysis of caseins

Capillary electrophoresis using a hydrophilically coated capillary and a low pH buffer containing urea has been used to follow the proteolytic action of plasmin and chymosin on isolated casein fractions, whole casein and individual milk samples selected on the basis of their genetic variants. Several of the main casein breakdown products were identified. These included, among others, γ1-casein (CN) A1, γ1-CN A2, γ1-CN B, γ1-CN C, γ2-CN A, γ1-CN A3, γ2-CN B, γ3-CN A and γ3-CN B, as well as proteose peptones, arising from the action of plasmin on the different genetic variants of β-CN. αs1-I-CN and αs1-CN f(1-23) from αs1-CN, and para-κ-CN and caseinomacropeptide from κ-CN produced by chymosin action were also separated. The knowledge of their migration times provided information on the extent and origin of casein hydrolysis in both milk and cheese, as found in samples of proteolysed milk or fresh cheese.
Prenatal Exposures to Perfluoroalkyl Acids and Associations with Markers of Adiposity and Plasma Lipids in Infancy: An Odense Child Cohort Study

Background: Perfluoroalkyl acids (PFAA) are repellants that cross the placental barrier, enabling interference with fetal programming. Maternal PFAA concentrations have been associated with offspring obesity and dyslipidemia in childhood and adulthood, but this association has not been studied in infancy. Objectives: We investigated associations between maternal PFAA concentrations and repeated markers of adiposity and lipid metabolism in infancy. Methods: In the prospective Odense Child Cohort, maternal pregnancy serum concentrations of five PFAA: Perfluorohexane sulfonic acid (PFHxS), perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorodecanoic acid (PFDA) were measured in 649 women. Offspring were examined at birth (n=613) and at 3 months (n=602) and 18 months (n=503) of age. Total cholesterol, LDL, HDL, and triglyceride were evaluated at 3 months (n=262) and 18 months (n=198) of age. Mixed effects linear regression models estimated associations between PFAA and standardized (SDS) body mass index (BMI), ponderal index, and waist circumference. Associations between PFAA and body fat% (BF%) and plasma lipids SDS at 3 months and 18 months of age were investigated with linear regression models. Results: PFNA and PFDA were associated with higher BMI SDS [adjusted β=0.26; 95% confidence interval (CI): 0.03, 0.49 and β=0.58; 95% CI: –0.03, 1.19, respectively, for 1-ng/mL increases] and ponderal index SDS (β=0.36; 95% CI: 0.13, 0.59 and β=1.02; 95% CI: 0.40, 1.64, respectively) at 3 and 18 months of age (pooled) in girls. Corresponding estimates for boys were closer to the null but not significantly different from estimates for girls. In boys and girls (combined), PFNA and PFDA were associated with BF% at age 3 months (for 1-ng/mL PFDA, β=0.40; 95% CI: 0.04, 0.75), and PFDA was associated with total cholesterol SDS at 18 months (β=1.06; 95% CI: 0.08, 2.03) (n=83). Discussion: Prenatal PFAA were positively associated with longitudinal markers of adiposity and higher total cholesterol in infancy. These findings deserve attention in light of rising rates of childhood overweight conditions and dyslipidemia. https://doi.org/10.1289/EHP5184
Anti-Bacterial and Anti-Fouling Capabilities of Poly(3,4-Ethylenedioxythiophene) Derivative Nanohybrid Coatings on SUS316L Stainless Steel by Electrochemical Polymerization

We have successfully fabricated poly(3,4-ethylenedioxythiophene) (PEDOT) derivative nanohybrid coatings on flexible SUS316L stainless steel by electrochemical polymerization, which can offer anti-fouling and anti-bacterial capabilities. PEDOT derivative nanohybrids were prepared from polystyrene sulfonates (PSS) and graphene oxide (GO) incorporated into a conducting polymer of PEDOT. Additionally, the negative charge of the PEDOT/GO substrate was further modified by poly-diallyldimethylammonium chloride (PDDA) to form a positively charged surface. These PEDOT derivative nanohybrid coatings could provide a straightforward means of controlling the surface energy, roughness, and charges with the addition of various derivatives in the electrochemical polymerization and electrostatically absorbed process. The characteristics of the PEDOT derivative nanohybrid coatings were evaluated by Raman spectroscopy, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), water contact angle, and surface potential (zeta potential). The results show that PEDOT/PSS and PEDOT/GO nanohybrid coatings exhibit excellent anti-fouling capability. Only 0.1% of bacteria can be adhered on the surface due to the lower surface roughness and negative charge surface by PEDOT/PSS and PEDOT/GO modification. Furthermore, the anti-bacterial capability (7 mm of inhibition zone) was observed after adding PDDA on the PEDOT/GO substrates, suggesting that the positive charge of the PEDOT/GO/PDDA substrate can effectively kill bacteria (Staphylococcus aureus). Given their anti-fouling and anti-bacterial capabilities, PEDOT derivative nanohybrid coatings have the potential to be applied to biomedical devices such as cardiovascular stents and surgical apparatus.