Miracle-dz/UniFAR-FTUs huggingface dataset:
Perturbation of ATG16L1 function impairs the biogenesis of Salmonella and Coxiella replication vacuoles

Anti‐bacterial autophagy, known as xenophagy, is a host innate immune response that targets invading pathogens for degradation. Some intracellular bacteria, such as the enteric pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), utilize effector proteins to interfere with autophagy. One such S. Typhimurium effector, SopF, inhibits recruitment of ATG16L1 to damaged Salmonella‐containing vacuoles (SCVs), thereby inhibiting the host xenophagic response. SopF is also required to maintain the integrity of the SCV during the early stages of infection. Here we show disruption of the SopF‐ATG16L1 interaction leads to an increased proportion of cytosolic S. Typhimurium. Furthermore, SopF was utilized as a molecular tool to examine the requirement for ATG16L1 in the intracellular lifestyle of Coxiella burnetii, a bacterium that requires a functional autophagy pathway to replicate efficiently and form a single, spacious vacuole called the Coxiella‐containing vacuole (CCV). ATG16L1 is required for CCV expansion and fusion but does not influence C. burnetii replication. In contrast, SopF did not affect CCV formation or replication, demonstrating that the contribution of ATG16L1 to CCV biogenesis is via its role in autophagy, not xenophagy. This study highlights the diverse capabilities of bacterial effector proteins to dissect the molecular details of host–pathogen interactions.
Simultaneous analysis of antigen‐specific B and T cells after SARS‐CoV‐2 infection and vaccination

Conventional methods for quantifying and phenotyping antigen-specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen-specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen-specific T and B cells would allow for more thorough immune profiling with significantly reduced sample requirements. To this end, we developed the B And T cell Tandem Lymphocyte Evaluation (BATTLE) assay, which allows for the simultaneous identification of SARS-CoV-2 Spike reactive T and B cells using an optimized Activation Induced Marker (AIM) T cell assay and dual-color B cell antigen probes. Using this assay, we demonstrate that antigen-specific B and T cell subsets can be identified simultaneously using conventional flow cytometry platforms and provide insight into the differential effects of mRNA vaccination on B and T cell populations following natural SARS-CoV-2 infection.
Human herpesvirus 8-associated multicentric Castleman disease in a patient with advanced HIV infection

Abstract Rational: Multicentric Castleman disease (MCD) is a nonclonal lymphoproliferative disorder that is rarely reported from Southeast Asian countries. Here, we report a case of human herpesvirus 8 (HHV-8)-associated MCD in a patient with advanced human immunodeficiency virus (HIV) infection who presented with prolonged intermittent fever, urticarial rash, hepatosplenomegaly, and generalized lymphadenopathy. Patient concerns: A 34-year-old man with advanced HIV infection who was in good compliance with his antiretroviral treatment regimen presented with intermittent fever, weight loss, marked hepatosplenomegaly, and generalized lymphadenopathy. Recurrent symptoms of high-grade fever, abdominal discomfort, pancytopenia, and high C-reactive protein level occurred for 16 months. Diagnoses: Histopathological findings of left inguinal lymph node revealed diffuse effacement of lymph node architecture with coexpression of HHV-8 latency-associated nuclear antigen 1 from immunohistochemical staining. The HHV-8 viral load was 335,391 copies/mL. Interventions: The patient was treated initially with one dose of intravenous rituximab (375 mg/m2) followed by subcutaneous rituximab (1400 mg) weekly for 5 weeks. Outcomes: The patient's recurrent systemic symptoms subsided dramatically, and he has now been in remission for almost two years. Lessons: HHV8-associated MCD remains a diagnostic challenge in advanced HIV disease and should be suspected in those with recurrent flares of systemic inflammatory symptoms. Lymph node histopathology is essential for diagnosis and for excluding clonal malignancy. HHV-8 viral load is also useful for diagnosis and for monitoring disease activity.
Understanding the Dominant Moisture Sources and Pathways of Summer Precipitation in the Southeast Prairie Pothole Region

Summer rainfall in the southeast Prairie Pothole Region (SEPPR) is an important part of a vital wetland ecosystem that various species use as their habitat. We examine sources and pathways for summer rainfall moisture, large‐scale features influencing moisture delivery, and large‐scale connections related to summer moisture using the Hybrid Single‐Particle Lagrangian Integrated Trajectory (HYSPLIT) model. Analysis of HYSPLIT back trajectories shows that land is the primary moisture source for summer rainfall events indicating moisture recycling plays an important role in precipitation generation. The Great Plains Low‐Level Jet/Maya Express is the most prominent moisture pathway. It impacts events sourced by land and the Gulf of Mexico (GoM), the secondary moisture source. There is a coupling between land, atmosphere, and ocean conveyed by large‐scale climate connections between rainfall events and sea surface temperature (SST), Palmer Drought Severity Index, and 850‐mb heights. Land‐sourced events have a connection to the northern Pacific and northwest Atlantic Oceans, soil moisture over the central U.S., and low‐pressure systems over the SEPPR. GoM‐sourced events share the connection to soil moisture over the central U.S. but also show connections to SSTs in the North Pacific and Atlantic Oceans and the GoM, soil moisture in northern Mexico, and 850‐mb heights in the eastern Pacific Ocean. Both types of events show connections to high 850‐mb heights in the Caribbean which may reflect a connection to Bermuda High. These insights into moisture sources and pathways can improve skill in SEPPR summer rainfall predictions and benefit natural resource managers in the region.
Cardioprotective potential of lithium and role of fractalkine in euthymic patients with bipolar disorder

Objective: Over a half century, lithium has been used as the first-line medication to treat bipolar disorder. Emerging clinical and laboratory studies suggest that lithium may exhibit cardioprotective effects in addition to neuroprotective actions. Fractalkine (CX3CL1) is a unique chemokine associated with the pathogenesis of mood disorders and cardiovascular diseases. Herein we aimed to ascertain whether lithium treatment is associated with favorable cardiac structure and function in relation to the reduced CX3CL1 among patients with bipolar disorder. Methods: We recruited 100 euthymic patients with bipolar I disorder aged over 20 years to undergo echocardiographic study and measurement of plasma CX3CL1. Associations between lithium treatment, cardiac structure and function and peripheral CX3CL1 were analyzed according to the cardiovascular risk. The high cardiovascular risk was defined as (1) age ⩾ 45 years in men or ⩾ 55 years in women or (2) presence of concurrent cardiometabolic diseases. Results: In the high cardiovascular risk group (n = 61), patients who received lithium as the maintenance treatment had significantly lower mean values of left ventricular internal diameters at end-diastole (Cohen’s d = 0.65, p = 0.001) and end-systole (Cohen’s d = 0.60, p = 0.004), higher mean values of mitral valve E/A ratio (Cohen’s d = 0.51, p = 0.019) and superior performance of global longitudinal strain (Cohen’s d = 0.51, p = 0.037) than those without lithium treatment. In addition, mean plasma levels of CX3CL1 in the high cardiovascular risk group were significantly lower among patients with lithium therapy compared with those without lithium treatment (p = 0.029). Multiple regression models showed that the association between lithium treatment and mitral value E/A ratio was contributed by CX3CL1. Conclusion: Data from this largest sample size study of the association between lithium treatment and echocardiographic measures suggest that lithium may protect cardiac structure and function in patients with bipolar disorder. Reduction of CX3CL1 may mediate the cardioprotective effects of lithium.
SHOCK INITIATION THRESHOLDS FOR INSENSITIVE HIGH EXPLOSIVES

Work with conventional high explosives shows that the initiation thresholds for a variety of projectile types can be mapped onto a single curve. This curve is distinct from, although parallel to, the Pop Plot curve. In contrast for an insensitive high explosive, the location of the initiation thresholds appear divided between those forming a distinct threshold curve and those that appear to be a continuation of the Pop Plot. Projectile diameters larger than the failure diameter still form a distinct threshold, but smaller projectile diameters lie on the Pop Plot curve. Impacts lying on the threshold curve produce non‐divergent detonations which in many cases appear likely to remain non‐divergent or even fade. Those initial conditions that lie on the Pop Plot only need sufficient run distance in the explosive before a diverging detonation is achieved.
SLCO4A1 is a Prognosis-Associated Biomarker Involved in Neutrophil-Mediated Immunity in Thyroid Cancer

Objective The study aimed to investigate the value of solute carrier organic anion transporter family member 4A1 (SLCO4A1) in thyroid cancer mainly from three aspects: expression, prognosis, and biological function analyses. Methods Based on various bioinformatic approaches, genes co-expressed with vascular endothelial growth factor C (VEGFC) in thyroid cancer were used for further survival and expression analyses to identify the target gene. After evaluation of the SLCO4A1 expression levels in thyroid cancer, Cox regression analysis was utilized to predict the risk factors for survival of thyroid cancer patients. And receiving operating characteristic curve analysis was performed to validate the prognostic value of SLCO4A1. Additionally, WebGestalt was employed for enrichment analysis of SLCO4A1 and its co-expressed genes. Further, the relation between SLCO4A1 and neutrophil was analyzed, followed by exploring the association of SLCO4A1 with immunomodulators. Results A total of 38 consistent VEGFC co-expressed genes were generated, and SLCO4A1 was selected as the target gene due to its oncogenic characteristics. SLCO4A1 was highly expressed in thyroid cancer at both gene and protein levels, and SLCO4A1 mRNA expression was significantly associated with the cancer stage (all P <0.05). Besides, high SLCO4A1 expression led to unfavorable progression-free survival (PFS) of thyroid cancer patients (P =0.0066). Further, Cox regression analysis indicated that high SLCO4A1 expression was an independent predictor of poor PFS in patients with papillary thyroid cancer, particularly in patients at stage 1 and female patients (all P <0.001). The enrichment analysis results showed that SLCO41A was involved in the neutrophil-mediated immunity pathway. Moreover, SLCO4A1 had a positive relation with neutrophils (all P <0.05). Finally, a significant correlation between SLCO4A1 and immunomodulators was observed (all P <0.001). Conclusion SLCO4A1 was a potential prognostic biomarker for papillary thyroid cancer patients. And SLCO4A1 might affect PFS in thyroid cancer patients by positive regulation of neutrophil-mediated immunity pathway.
300 GHz InP HBT amplifier with 10 mW output power

A high-power terahertz solid-state amplifier fabricated using 0.25 μm InP heterojunction bipolar transistor (HBT) technology is reported. This amplifier utilies a novel defective-ground four-way balun to combine differential amplifier chains for a total output device periphery of 40 μm. A significant amount of power of ∼10 mW is obtained at 305 GHz with better than 20 dB small-signal gain.
Dolphins, whales and porpoises : 2002-2010 conservation action plan for the world's cetaceans

Consistent evaluation and new recommendations for action are required of protective measures to address threats that were unrecognised or non-existent until recently. Global warming, noise pollution and reduced availability of prey are now of great concern. The all too familiar threats of accidental killing in fishing gear and exposure to toxic chemicals remain almost intractable. This Action Plan reviews threats and offers possible solutions. It also contains a thorough review of the status of species and a list of 57 recommended research projects and education initiatives
Achieving Organizational Learning Through Team Competition

Abstract: This article contributes insights that engineering managers can use to cultivate organizational learning through competition. Contemporary firms face the challenge of successfully translating quality improvements into sustainable competitive advantages. Organizational learning is crucial to making quality improvements sustainable. This case study examined the mechanisms used to achieve organizational learning in a large-scale quality improvement competition. Thirty-four project teams from an international electronics company were included in this study. The results show that benchmarking and retrieval of electronic information were the most common methods used to acquire learning inputs. Knowledge transfer was most frequently achieved through training and adoption of internal specifications. Finally, learning was most frequently applied through formal changes to manufacturing methods and project management procedures.
Non Epitheliotropic B-Cell Lymphoma with Plasmablastic Differentiation vs. Cutaneous Plasmacytosis in a 12-Years-Old Beagle: Case Presentation and Clinical Review

Cutaneous lymphoid neoplasms and cutaneous plasmacytosis are rare in the dog; in human and in veterinary medicine, these have many clinical, cytological, histological, and phenotypic similarities, and a diagnosis of certainty is not easy. The aim of this study is to describe a case of cutaneous non epitheliotropic B-cell lymphoma (CNEBL) with plasmablastic differentiation vs. multiple cutaneous plasmacytosis (CP) in a dog, since the scarce bibliographic data on these topics. A 12-year-old male Beagle dog was presented for multiple, nodular, cutaneous, and subcutaneous, indolent masses disseminated on the whole body. Cytological, histological, flow cytometric, and immunohistochemical examinations, as well as complete radiographic evaluation, echocardiography, and abdominal ultrasound were performed. Cytology, histopathology, flow cytometric, and immunohistochemical examination, performed on the skin lesions, revealed a B-cell phenotype with plasmablastic differentiation. Nevertheless, a final diagnosis could not be achieved and it was categorized as a case of borderline CNEBL with plasmablastic differentiation versus CP. The dog was treated with a COP chemotherapeutic protocol. Total remission was obtained and relapse occurred 120 days later. To our knowledge, specific markers are actually unavailable to certainly differentiate CNEBL and CP in the dog and future studies are needed to improve knowledge on these pathologies in veterinary medicine, since prognosis and therapy are different.
Improving the applicability of workload control (WLC): the influence of sequence-dependent set-up times on workload controlled job shops

Simulation has demonstrated that the workload control (WLC) concept can improve performance in job shops, but positive empirical results are scarce. A key reason for this is that the concept has not been developed to handle a number of practical considerations, including sequence-dependent set-up times. This paper investigates the influence of sequence-dependent set-up times on the performance of a workload-controlled job shop. It introduces new set-up-oriented dispatching rules and assesses the performance impact of controlled order release. Simulation results demonstrate that combining an effective WLC order release rule with an appropriate dispatching rule improves performance over use of a dispatching rule in isolation when set-up times are sequence dependent. The findings improve our understanding of how this key implementation challenge can be overcome. Future research should investigate whether the results hold if set-up time parameters are dynamic and set-up times are not evenly distributed across resources.
Diseases 2.0: a weekly updated database of disease–gene associations from text mining and data integration

The scientific knowledge about which genes are involved in which diseases grows rapidly, which makes it difficult to keep up with new publications and genetics datasets. The DISEASES database aims to provide a comprehensive overview by systematically integrating and assigning confidence scores to evidence for disease–gene associations from curated databases, genome-wide association studies (GWAS), and automatic text mining of the biomedical literature. Here, we present a major update to this resource, which greatly increases the number of associations from all these sources. This is especially true for the text-mined associations, which have increased by at least 9-fold at all confidence cutoffs. We show that this dramatic increase is primarily due to adding full-text articles to the text corpus, secondarily due to improvements to both the disease and gene dictionaries used for named entity recognition, and only to a very small extent due to the growth in number of PubMed abstracts. DISEASES now also makes use of a new GWAS database, TIGA, which considerably increased the number of GWAS-derived disease–gene associations. DISEASES itself is also integrated into several other databases and resources, including GeneCards/MalaCards, Pharos/TCRD, and the Cytoscape stringApp. All data in DISEASES is updated on a weekly basis and is available via a web interface at https://diseases.jensenlab. org, from where it can also be downloaded under open licenses.
α-Synuclein Overexpression Increases Dopamine D2/3 Receptor Binding and Immune Activation in a Model of Early Parkinson’s Disease

Progressive degeneration of dopaminergic neurons, immune activation, and α-synuclein pathology characterize Parkinson’s disease (PD). We previously reported that unilateral intranigral injection of recombinant adeno-associated viral (rAAV) vectors encoding wild-type human α-synuclein produced a rat model of early PD with dopamine terminal dysfunction. Here we tested the hypothesis that decreases in dopamine result in increased postsynaptic dopamine D2/D3 receptor expression, neuroinflammation, and reduced synaptic vesicle glycoprotein 2A (SV2A) density. Rats were injected with rAAV encoding α-synuclein or green fluorescent protein and subjected to non-pharmacological motor tests, before euthanization at 12 weeks post-injection. We performed: (1) in situ hybridization of nigral tyrosine hydroxylase mRNA, (2) HPLC of striatal dopamine content, and (3) autoradiography with [3H]raclopride, [3H]DTBZ, [3H]GBR12935, [3H]PK11195, and [3H]UCB-J to measure binding at D2/3 receptors, vesicular monoamine transporter 2, dopamine transporters, mitochondrial translocator protein, and SV2A, respectively. rAAV-α-synuclein induced motor asymmetry and reduced tyrosine hydroxylase mRNA and dopamine content in ipsilateral brain regions. This was paralleled by elevated ipsilateral postsynaptic dopamine D2/3 receptor expression and immune activation, with no changes to synaptic SV2A density. In conclusion, α-synuclein overexpression results in dopaminergic degeneration that induced compensatory increases in D2/3 binding and immune activation, recapitulating many of the pathological characteristics of PD.
Gratitude Moderates the Relation between Daily Hassles and Satisfaction with Life in University Students

Satisfaction with life as a judgmental cognitive process can be negatively influenced by appraisals of daily events such as hassles. Trait-gratitude—a tendency to appraise, recognize and respond to life events through being grateful—is a determinant of mental health and well-being, and has been shown to be related to the positive appraisal of life. The aim of the current study was to investigate the moderating role of trait-gratitude in the relationship between daily hassles and satisfaction with life. In the process of carrying out this study, the French version of the Gratitude Questionnaire (GQ-6) was validated. A total of 328 French undergraduates completed questionnaires measuring gratitude, satisfaction with life, and daily hassles to test the main hypothesis. They also completed optimism, coping strategies, depression, and anxiety questionnaires in order to assess the convergent validity of the French version of the GQ-6. First, the results showed satisfactory psychometric properties of the Gratitude Questionnaire. Second, the results indicated the moderating role of trait-gratitude in the relationship between daily hassles disturbance and satisfaction with life. This study further documents the role of gratitude as a determinant of well-being and provides French-speaking clinicians and researchers with a useful tool to measure grateful disposition.
A new MF and HF ground-wave model for urban areas

Following discussions (Luo Lichun 1997) on the propagation and regularities of HF ground waves in urban areas in China, and processing the HF experimental data obtained from 1993 through 1997 and MF data obtained in 1995 and 1997 in Beijing, a new model for MF and HF ground-wave propagation in urban areas is proposed in this paper. The key novelty in the proposed model is the inclusion of three building-complex parameters, r/sub b/, r/sub s/ and r/sub e/, and a new height-gain factor, G/sub h/. A qualitative derivation of these is offered. The method of selecting these building parameters is discussed. Some HF and MF ground-wave propagation losses, which occur under different conditions of the operating frequencies, heights, and locations of the transmitting antennas, are calculated by means of the new model and, for contrast, by means of some earlier models. The new model can be used to calculate either the coarse prediction of ground-wave loss, or to calculate fine predictions, even for a point site. The accuracy of the calculation using the new model is superior to that of the earlier models.