Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation, molecular characterization and antibiotic resistance of Shiga Toxin – Producing Escherichia coli (STEC) from buffalo in India

Signiﬁcance and Impact of the Study: The buffaloes from different districts of West Bengal, India, are important reservoir of multidrug-resistant Shiga toxin – producing Escherichia coli (STEC). India is home to more than 56% of world buffalo population, traditionally raised by farmers. So, there is a major risk of transmission of STEC among the human population of this part of the globe. However, there is no prevalence study of STEC from healthy or diarrhoeic buffalo in India. The present study reports for the ﬁrst time in India about isolation, molecular characterization and antibiotic resistance pattern of STEC in healthy buffaloes. Abstract In total, 363 Escherichia coli were isolated from 165 faecal samples of healthy buffaloes in West Bengal, India. Twenty-four of these isolates (6 (cid:1) 61%) were found to carry at least one gene characteristic for Shiga toxin – producing Escherichia coli (STEC). These STEC strains belonged to 13 different O-serogroups. The stx 1 gene was present in 23 (95 (cid:1) 8%) of total STEC isolates, whereas 20 (83 (cid:1) 3%) STEC isolates carried the gene stx 2 . Twelve strains of E. coli (50% of total STEC isolates) possessed enterohaemolysin ( ehxA ) gene in combination with others. Fourteen (58 (cid:1) 33%) isolates found to possess saa gene. However, no E. coli was detected harbouring gene for intimin protein ( eaeA ). Of 23 stx 1 -positive isolates, seven (30 (cid:1) 43%) were positive for genes of the stx 1C subtype. Of the 20 isolates with the stx 2 gene, 25% (5/20) possessed stx 2C and 10% (2/20) possessed stx 2d gene. The phylogenetic analysis after RAPD of STEC strains revealed six major clusters. The isolated STEC strains were resistant most frequently to erythromycin (95 (cid:1) 83%), cephalothin (62 (cid:1) 5%), amikacin (54 (cid:1) 17%), kanamycin (45 (cid:1) 83%) and gentamicin (41 (cid:1) 67%) group of antibiotics. No ESBL-producing ( bla CTXM , bla TEM , bla SHV ) or quinolone resistance gene ( qnrA ) was detected in the STEC isolates.
Comparing WorldView-2 and PlanetScope Imagery to Mapping Housing Types Using GEOBIA

The mapping accuracy of housing types plays a vital role in urban planning and development. Choosing the right imagery for urban geospatial analysis matters in terms of spatial or textural resolution. Here we compare the effectiveness of different satellite imagery, namely WorldView-2 (2m resolution) and PlanetScope (3m resolution) to map housing types. The segmentation algorithm employed is SNIC (Simple Non-Iterative Clustering) while SVM (Support Vector Machine) algorithm is for classification. This study assessed the performance of these satellite platforms in capturing to extract spatial and spectral elements of each housing class and differentiating between urban villages (Kampung Kota), government-based housing, and private-based gated housing classes in the Tangerang area. WorldView-2, with its high spatial resolution, provides detailed information, allowing for precise delineation of housing boundaries and distinctive features, whereas Planetscope imagery offers better textural information for the segmentation stage. Despite the coarser details, the SVM classification algorithm achieved an overall accuracy of 65.00% using PlanetScope imagery. Comparative analysis revealed that WorldView-2 imagery outperformed PlanetScope imagery in terms of overall accuracy, with an overall accuracy of 65.52%. The higher spatial resolution of WorldView-2 enables better discrimination of housing types, resulting in more accurate classification. However, PlanetScope imagery provides valuable information, particularly for large-scale urban planning applications. The findings of this study contribute to the field of remote sensing and assist urban planners in making informed decisions regarding housing development and infrastructure planning based on available satellite imagery resources, both of which have their own advantages and disadvantages.
Model Test on Resistance in Ice-Free Ice Channels

During wintertime merchant ships in the Baltic Sea have to travel in ice-covered waters and mainly in brash ice channels. Thus the channel resistance is one of the important design criteria. A normal assumption is that the total resistance in a channel can be calculated by superposing the open water resistance and the resistance due to ice. The aim of this work was to study the affects of channel width, ice thickness and ice cover stiffness on the channel resistance by the means of model tests.
Tumor-selective effects of active RAS inhibition in pancreatic ductal adenocarcinoma

Broad-spectrum RAS inhibition holds the potential to benefit roughly a quarter of human cancer patients whose tumors are driven by RAS mutations. However, the impact of inhibiting RAS functions in normal tissues is not known. RMC-7977 is a highly selective inhibitor of the active (GTP-bound) forms of KRAS, HRAS, and NRAS, with affinity for both mutant and wild type (WT) variants. As >90% of human pancreatic ductal adenocarcinoma (PDAC) cases are driven by activating mutations in KRAS, we assessed the therapeutic potential of RMC-7977 in a comprehensive range of PDAC models, including human and murine cell lines, human patient-derived organoids, human PDAC explants, subcutaneous and orthotopic cell-line or patient derived xenografts, syngeneic allografts, and genetically engineered mouse models. We observed broad and pronounced anti-tumor activity across these models following direct RAS inhibition at doses and concentrations that were well-tolerated in vivo. Pharmacological analyses revealed divergent responses to RMC-7977 in tumor versus normal tissues. Treated tumors exhibited waves of apoptosis along with sustained proliferative arrest whereas normal tissues underwent only transient decreases in proliferation, with no evidence of apoptosis. Together, these data establish a strong preclinical rationale for the use of broad-spectrum RAS inhibition in the setting of PDAC.
Machine Learning-Enhanced Microfluidic SIW Sensor for Water-in-Fuel Detection Applications

This paper presents a half-mode substrate-integrated waveguide (HMSIW) sensor with a microfluidic integrated channel for detecting microliter water-in-diesel at 7.1 GHz. A microfluidic channel is embedded into the HMSIW structure, allowing interaction with the fields of TE103 mode. The sensor's performance is assessed by following the resonance frequency fluctuations as water concentration varies in a $65\ \mu\mathrm{L}$ sample using an examination of dielectric permittivity behavior. The resonance frequency is highly sensitive, shifting by 6.4 MHz per 0.05% water content. A back-propagation artificial neural network (BP-ANN) model is also deployed to allow cognitive capabilities, attaining a stunning detection range down to 0.01%. This sensor offers a potential option for real-time monitoring and quality control in fuel systems because to its multipurpose capabilities.
Numerical simulation of zonal distrigation of surrounding rock mass in deep mine roadways

The zonal disintegration of surrounding rock mass of roadway was investigated using finite difference code FLAC.The generation and evolution of zonal disintegration were analyzed as follows:with the relaxation of initial stresses resulting from the excavation of roadway,numerous fracture ribbons extend from the surface and the vicinity of roadway to deeper fields of surrounding rock mass,within this process,one fracture ribbon may split in two or more fracture ribbons.These ribbons intersect with each other.Consequently,net fracture spacing is formed in the vicinity of roadway and ring fracture spacing formed in the deeper fields of surrounding rock mass.The level of initial stress before the roadway is excavated,as well as the lateral pressure and the section shape of roadway,all have significant effects on the characteristics of zonal disintegration.The results of numerical simulation are in good agree with the in-situ monitoring results from former scholars.
Raman spectroscopic thermometry of carbonaceous material in chondrites: four–band fitting analysis and expansion of lower temperature limit

*Geochemical Research Center, Graduate School of Science, The University of Tokyo, Hongo, Tokyo 113–0033, Japan **Department of Earth and Planetary Science, Graduate School of Environmental Studies, Nagoya University, Nagoya, Aichi 464–8601, Japan ***Department of Earth and Planetary Science, Graduate School of Science, The University of Tokyo, Hongo, Tokyo 113–0033, Japan Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan
The Tectonic Framework of Assam

A spur of ancient rocks partly covered by gently-dipping Tertiary beds extends from the Shillong Plateau and Mikir Hills north-eastwards beneath the alluvium of Upper Assam. Over this spur the Eastern Himalaya have been thrust southwards and the Naga Hills have been thrust north-westwards. The amount of movement of the overthrust masses cannot be determined but it is suggested that in each case the total displacement may be 150-300 kilometres or even more. The Shillong Plateau is separated from the Surma Valley by a faulted monocline with southerly dips. This fault, the Dauki tear-fault, is now shown to have a probable horizontal displacement of about 250 kilometres, and thus to be a major feature of the tectonic pattern of the Indian sub-continent. The horizontal movement along the Dauki tear-fault detached the Shillong Plateau from the main mass of the Indian Shield. The principal movements occurred late in the Tertiary, mostly in the Pliocene.
The Influence of Current Ratio (CR), Return of Assets (ROA), Company Size and Sales Growth on Capital Structure in Companies Registered on JII for the 2015-2021 Period

This study aims to analyze the effect of the Current Ratio (CR), Return Of Assets (ROA), Company Size (size) and Sales Growth (Growth) on the Capital Structure of companies listed on JII for the 2015-2021 period. The sample in this study were 10 companies. The data used in this study comes from secondary data obtained from the company's financial statements. Related to data analysis, this study uses linear regression analysis of panel data with the Random Effect Model (REM) model. It is known that the results obtained in this study are Current Ratio (CR) having a negative and significant effect on capital structure by proxies Debt to Equity Ratio (DER), Return Of Assets (ROA), and Firm Size having a positive and significant effect on capital structure by Debt to Equity Ratio (DER) proxy and Sales Growth has no effect on capital structure. Therefore, companies can increase their Current Ratio (CR), Return Of Assets (ROA), and Company Size so that the company's capital structure can be used optimally and efficiently.
LEFT ATRIAL APPENDAGE CLOSURE IN PATIENTS WITH MECHANICAL MITRAL VALVE PROSTHESIS: A MULTICENTER ITALIAN PILOT STUDY

Background: In patients with atrial fibrillation (AF) on vitamin K antagonist (VKA) therapy and therapeutical INR range the incidence of cardiac thromboembolism is not negligible and the subgroup carrying a mechanical prosthetic mitral valve (PMV) has the highest risk. We aimed to assess the long-term effects of left atrial appendage closure (LAAC) in AF patients carriers of mechanical PMV who experienced a failure of VKA therapy. Methods: In this retrospective, multicenter study, patients who underwent LAAC because of thrombotic events including TIA/stroke, systemic embolism and evidence of left atrial appendage thrombosis/sludge during VKA therapy were enrolled. Patients with mechanical PMV were included and compared with controls. The primary endpoint was the composite of all-cause death, major cardiovascular events and major bleedings at follow-up. Feasibility and safety of LAAC was also assessed. Results: A total of 55 patients (42% females; mean age 70 {+/-} 9 years) including 12 carriers of mechanical PMV were enrolled. The most frequent indication to LAAC (71%) was LAA thrombosis or sludge. Procedural success was achieved in 96% of overall cases and in 100% of patients with PMV. In 35 patients a cerebral protection device was used. During a median follow-up of 6.1 {+/-} 4.3 years, 4 patients with PMV and 20 patients without PMV reported adverse events (HR 0.73 [95% CI 0.25 - 2.16, p=0.564]). Conclusion: LAAC seems to be a valuable alternative in AF patients with failure of VKA therapy who are carriers of mechanical PMV. This off-label, real-world clinical practice indication deserve validation in further studies.
Met Allele of BDNF Gene is Protective Factor Against Age-Related Decline in Left Hemisphere Short-Term Verbal Memory

Since brain-derived neurotrophic factor (BDNF) is crucial for neuroplasticity of adult brain, functional polymorphism Val66Met of the BDNF gene may contribute to cognitive heterogeneity in later life. Initially, the Met allele was considered as “risk” allele due to its role in reducing BDNF release leading to detrimental effects in neurodegenerative diseases. Recently, impact of the Met allele is reconsidered as neuroprotective in aging due to negative effect of Val allele form of proBDNF. We explored whether the Met allele protects against the age-related decline in hemispheric efficacy of short-term verbal memory and whether this effect is moderated by high intellectual environment of professional activity (science vs non-science) in younger ($\mathrm{N}=251$, 18-35 years) and high-functional older ($\mathrm{N}=124$, 55-80 years) adults. Regardless of intellectual environment, genotypes of the BDNF gene showed different age-associated trends in recall of the right-ear presented words (addressed to the left hemisphere): significant downtrend in Val/Val (younger>older) and no age-related difference in Met carriers. Negative correlation between recalled right- and left-presented words in all groups except older Met-carriers may suggest changed hemispheric communication in the last group. The results bring evidence that the Met allele may act to prevent age-related decline in left hemisphere short-term verbal memory.
Sensory Outcomes after Neurotization in Nipple-sparing Mastectomy and Implant-based Breast Reconstruction

Background: Mastectomy and breast reconstruction techniques continue to evolve to optimize aesthetic and reconstructive outcomes. However, the loss of sensation after mastectomy remains a major limitation. This article describes our evolution of a novel approach that we first described in 2019, combining recent advances in breast oncologic, reconstructive, and peripheral nerve surgery to optimize sensory outcomes. Methods: Nipple-sparing mastectomy was performed in all patients and involved preservation of lateral intercostal nerves when anatomy was favorable. When nerves could not be preserved without compromising oncologic safety, nipple–areolar complex neurotization was performed using allograft or intercostal autograft from a transected T3, T4, or T5, lateral intercostal nerve to identified subareolar nerve targets. Immediate, prepectoral, direct-to-implant reconstruction was then performed. Acroval one-point moving and one-point static pressure thresholds established baseline sensibility values, which were then repeated at multiple time points postoperatively. Results: Outcomes from 47 women (79 breasts) were assessed prospectively. Mean follow-up was 9.2 months (range 6–14 months). At 6 months postoperatively, over 80% of patients had good-to-excellent one-point moving as well as one-point static sensibility scores averaged across all areas tested. None of the patients developed persistent dysesthesia or clinical evidence of neuroma. Conclusions: This study represents the largest series reported to date of sensibility outcomes after nipple-sparing mastectomy and implant reconstruction with concurrent neurotization. Sensibility results show that this approach allows for preservation of high degrees of breast and nipple–areolar complex sensation in most patients.
Quintessenz Journals

Despite the known influence of orthodontic treatment on facial appearance, there have been few comparative studies of the effect of different treatments, and none of these appear to have looked at the long-term consequences. This study compared the effect of traditional fixed appliances and orthotropic (growth guidance) treatment without fixed appliances on a series of 12 identical twins, 10 years after treatment. Facial changes were assessed by a panel of 12 lay judges. A comparison was also made of the dental changes and an error study undertaken. The results showed that most of the traditionally treated twins were judged to look less attractive after treatment, while most treated by orthotropics were judged to have improved. There was little difference in the dental results, but the traditionally treated cases seemed to relapse more frequently. World J Orthod 2007;8:174–188. John Mew, BDS (Lond), LDS, RCS Eng, MFGDP (UK), MOrth RCS (Edin)1
Analysis of Total Flavonoid and Phenol Content from the Combination of Red Spinach (Amaranthus tricolor L.) Ethanolic Extract and Chrysanthemum Flower (Chrysanthemum morifolium) Ethanolic Extract as a Potential Anti-anemic

Red spinach and chrysanthemum are two plants that contain various active compounds, including flavonoids and phenols which have good antioxidant activity. One of the benefits of antioxidants is as an anti-anemia. The combination of red spinach and chrysanthemum extracts is expected to be a potential alternative treatment for anemia by combining high Fe and antioxidants found in both herbal ingredients. Research related to test the levels of flavonoids and phenols in red spinach and chrysanthemum extracts (in combination) has not been carried out. This study aimed to measure the total flavonoid and phenol levels of the combination of red spinach ethanol extract (EEBM) and chrysanthemum flower ethanol extract (EEBK). The design of this research is descriptif quantitative. The method used for the extraction is maceration. The solvent used is 70% ethanol. Then the two extracts were combined by mixing the two extracts with a ratio of 5.6 ml EEBM and 1.4 ml EEBK. The process of mixing the extracts was carried out by the centrifugation method. The suspension of the combination of EEBM and EEBK was then measured for the total flavonoid and phenol content. Determination of total flavonoid content (TFC) was carried out using UV-Vis spectrophotometry method with AlCl3 reagent at 425 nm. TFC results are expressed in quercetin equivalent (QE). The TFC result from the combination of EEBM and EEBK was 85.33 mg QE/g. Determination of total phenol content (TPC) was carried out using UV-Vis spectrophotometry method with Folin-Ciocalteu LP reagent at 735 nm. TPC results are expressed in gallic acid equivalent (GAE). The TPC result from the combination of EEBM and EEBK is 25.22 GAE/g. Flavonoids and phenols contained in the combination of EEBM + EEBK extracts can be an alternative anti-anemia because they have the effect of helping the absorption of iron in the intestine
Prevalence of Post-traumatic Stress Disorder Symptoms Among Psychiatric Patients during the COVID-19 Pandemic

Background: The outbreak of the COVID-19 pandemic has caused extensive public health concerns, posing significant challenges to healthcare services. One particular area of concern is the mental health of psychiatric patients, who are often a neglected group. The aim of this study was to investigate the prevalence of, and associated factors for symptoms of post-traumatic stress disorder (PTSD) among psychiatric patients in China during the COVID-19 pandemic. Methods: Self-reported questionnaires were distributed to patients in four psychiatric hospitals in Beijing, China, between April 28th and May 30th, 2020. Information regarding sociodemographic characteristics, COVID-19 related factors, support, psychosomatic factors, and PTSD symptoms was collected data using a series of scales, such as the Impact of Event Scale-Revised, the 7-item Generalized Anxiety Disorder Scale, the 9-item Patient Health Questionnaire depression scale, and so on. Multivariate regression was used to identify factors related to PTSD symptoms. Results: A total of 1,055 psychiatric patients were included in the final sample. The prevalence of PTSD symptoms was 41.3%. Hierarchical linear regression demonstrated that fear of the pandemic and anxiety were shared associated factors for both symptoms of PTSD and its subscales. Additionally, age was an associated factor for the total PTSD (β = 0.12, p < 0.01), intrusion (β = 0.18, p < 0.001), and avoidance (β = 0.1, p < 0.05) symptoms; depression was an associated factor for the total PTSD s (β = 0.13, p < 0.001), intrusion (β = 0.11, p < 0.01), and hyperarousal (β = 0.19, p < 0.001) symptoms. Conclusions: The prevalence of PTSD symptoms was high among psychiatric patients during the COVID-19 pandemic in China. This study found that age, fear of the pandemic, anxiety and depression are significant associated factors of PTSD symptoms in psychiatric patients during the pandemic. We call for higher awareness and introduction of PTSD interventions to relieve the psychological stress in these patients.
Prevalence of Post Traumatic Stress Disorder (PTSD) in Internally Displaced People from Khartoum State After Sudan War in April., 2023

Internal displacement is emerging as one of the greatest challenges facing humanity. The displacement of people leads to various mental health problems such as PTSD, depression and anxiety in these groups of people, many studies have shown that PTSD is one of the most common psychiatric disorders diagnosed in displaced persons. This study aim to assess prevalence of PTSD in internally displaced people from Khartoum after Sudan war to orient the efforts toward the manipulation factors influencing their psychological health. This study is a descriptive cross-sectional community based study carry out among all people above 18 years old & internally displaced from Khartoum state by using self administrated questionnaire adapted from PCL-5 for assessed the 20 DSM-5 symptom of PTSD. The sample size was 666 participant, 50.8% of them were male, and most of them were 18 -25 years old (54.1%), displaced from Khartoum to Gaziera state in April (48.6%, 23.9%, 37.8%) respectively, also we founded that prevalence rate of PTSD was 311, 46.7% among the study sample and their was a significant relationship between PTSD and sex & age ( p value > 0.001, 0.010 respectively). The findings call for governments to adequately plan for and programme mental health interventions for internally displacement peoples.