Miracle-dz/UniFAR-FTUs huggingface dataset:
Epidemiologic significance of Toxoplasma gondii infections in turkeys, ducks, ratites and other wild birds: 2009–2020

Abstract Toxoplasma gondii infections are common in humans and animals worldwide. Wild and domestic avian species are important in the epidemiology of T. gondii infections because felids prey on them and excrete millions of oocysts in the environment, disseminating the infection. Herbivorous birds are also excellent sentinels of environmental contamination with T. gondii oocysts because they feed on the ground. Toxoplasma gondii infections in birds of prey reflect infections in intermediate hosts. Humans can become infected by consuming undercooked avian tissues. Here, the authors reviewed prevalence, persistence of infection, clinical disease, epidemiology and genetic diversity of T. gondii strains isolated from turkeys, geese, ducks, ratites and avian species (excluding chickens) worldwide 2009–2020. Genetic diversity of 102 T. gondii DNA samples isolated worldwide is discussed. The role of migratory birds in dissemination of T. gondii infection is discussed.
Poloxamer 188 Protects Isolated Adult Mouse Cardiomyocytes from Reoxygenation Injury

Reperfusion injury is a complex pathological event involving processes that can lead to further disruption of the cell membrane and function following an ischemic event. Return of blood flow allows for the needed reperfusion; however, for a period of time before remaining viable cells stabilize, reperfusion results in additional cellular injury. In cardiomyocytes, loss of membrane integrity allows abnormal influx of extracellular calcium, leading to hyper‐contracture and cell death. Methods to improve the membrane integrity of cardiomyocytes overwhelmed by pathological disruptions, such as reperfusion injury, are needed to prevent cell death, because of the myocardium's limited ability to regenerate. Research has shown administration of the copolymer P(oloxamer) 188 before ischemia/reperfusion can protect cardiomyocytes through membrane stabilization. This study sought to determine whether the administration of P188 at the beginning of the clinically more relevant time of reperfusion after ischemia will attenuate any additional damage to cardiomyocytes by stabilizing membrane integrity to allow the cells to maintain function. Using an in‐vitro cardiomyocyte model subjected to hypoxia/reoxygenation to simulate ischemia/reperfusion injury, we show that reoxygenation significantly potentiates the injury caused by hypoxia itself. P188, with its unique combination of hydrophobic and hydrophilic chemical properties, and only delivered at the beginning of reoxygenation, dose‐dependently protected cardiomyocytes from injury due to reoxygenation by repairing cell membranes, decreasing calcium influx, and maintaining cellular morphology. Our study also shows the hydrophobic portion of P188 is necessary for the stabilization of cell membrane integrity in providing protection to cardiomyocytes against reoxygenation injury.
Fast Screening Method For Polymorphisms in Exon 9 of the Catalase Gene.

We checked our simple screening technique for detection of the known polymorphism of rs769217, and the two acatalasemic mutations in exon 9 of the catalase gene. This fast and inexpensive method yielded better resolution than those of the standard SSCP. We suppose that the method detects the spontaneously formed single stranded DNAs. DOI : 10.14302/issn.2575-7881.jdrr-16-1375 Corresponding author: László Góth, Department of Medical Laboratory Analytics, Medical and Health Science Center, University of Debrecen, Debrecen POBox 55, Hungary H-4012. Phone:+36-52-451-331, Fax: +36-52-451-331, goth@med. unideb.hu Running title : Goth catalase exon 9
Shotgun metagenomics and microscopy indicate diverse cyanophytes, other bacteria, and microeukaryotes in the epimicrobiota of a northern Chilean wetland Nostoc (Cyanobacteria)

Prokaryotic Nostoc, one of the world's most conspicuous and widespread algal genera (similar to eukaryotic algae, plants, and animals) is known to support a microbiome that influences host ecological roles. Past taxonomic characterizations of surface microbiota (epimicrobiota) of free‐living Nostoc sampled from freshwater systems employed 16S rRNA genes, typically amplicons. We compared taxa identified from 16S, 18S, 23S, and 28S rRNA gene sequences filtered from shotgun metagenomic sequence and used microscopy to illuminate epimicrobiota diversity for Nostoc sampled from a wetland in the northern Chilean Altiplano. Phylogenetic analysis and rRNA gene sequence abundance estimates indicated that the host was related to Nostoc punctiforme PCC 73102. Epimicrobiota were inferred to include 18 epicyanobacterial genera or uncultured taxa, six epieukaryotic algal genera, and 66 anoxygenic bacterial genera, all having average genomic coverage ≥90X. The epicyanobacteria Geitlerinemia, Oscillatoria, Phormidium, and an uncultured taxon were detected only by 16S rRNA gene; Gloeobacter and Pseudanabaena were detected using 16S and 23S; and Phormididesmis, Neosynechococcus, Symphothece, Aphanizomenon, Nodularia, Spirulina, Nodosilinea, Synechococcus, Cyanobium, and Anabaena (the latter corroborated by microscopy), plus two uncultured cyanobacterial taxa (JSC12, O77) were detected only by 23S rRNA gene sequences. Three chlamydomonad and two heterotrophic stramenopiles genera were inferred from 18S; the streptophyte green alga Chaetosphaeridium globosum was detected by microscopy and 28S rRNA genes, but not 18S rRNA genes. Overall, >60% of epimicrobial taxa were detected by markers other than 16S rRNA genes. Some algal taxa observed microscopically were not detected from sequence data. Results indicate that multiple taxonomic markers derived from metagenomic sequence data and microscopy increase epimicrobiota detection.
PERFORMANCE OF SUNFLOWER IN RESPONSE TO NITROGEN MANAGEMENT AT DIFFERENT STAGES

day") also gave significantly highest values in the same treatment duringboth years. All the quality traits were significantly affected with the application of nitrogen in splits and had apositive correlation with the achene yield except achene oil content (%) and oleic acid (%) in oil.Keywords: Splitting, nitrogen, sunflower, achene, management
AC joint osteoarthritis: The role of genetics. An MRI evaluation of asymptomatic elderly twins

The anatomy of the articular surfaces has historically identified as major responsible for acromioclavicular joint osteoarthritis (ACJO). On the other side, the almost 100% prevalence of ACJO in subjects over 50 years old seems to suggest a multifactorial etiology. We compared ACJO between asymptomatic elderly monozygotic (MZ) and dizygotic (DZ) twins to investigate the influence of genetics and environmental factors.
Storage Period Affects Weight Loss of Japanese Quail Eggs

Long storage periods may increase embryo mortality. The objective of this study was to evaluate the effects of storage period on the weight loss, eggshell surface temperature, hatchability, and embryonic mortality of Japanese quail eggs. Two hundred fertile eggs were collected from a flock of 30-week-old Japanese quails (Coturnix coturnix japonica). The eggs were collected for 10 consecutive days after lay, and immediately incubated. A completely randomized experimental design with 10 treatments, corresponding to the number of days eggs were stored between egg collection and setting, with 20 replicates each, was applied. Egg weight loss increased with storage period duration, starting on day 6 (2.1%, on average) and reached 3.26%, on average, in eggs stored for 10 days. The highest hatchability (p>0.05) was obtained in eggs stored for two days, which also lost the least weight (1.20%). Storage period did not influence eggshell surface temperature (p>0.05) during incubation, but higher temperatures (p<0.05) were measured on days 10 and 15 of incubation compared with day 5. Eggs stored for ten days presented the highest weight loss, and therefore, a storage period of up to five days is recommended to maintain the quality of incubated Japanese quail eggs. Furthermore, egg surface temperature increases during the second half of the incubation period as a result of increasing embryonic metabolic rate.
Examining the Conceptual and Empirical Distinctiveness of Agreeableness and "Dark".

OBJECTIVE A growing research literature has focused on what have been termed "dark" personality traits/constructs. More recently, the "dark factor" of personality has been proposed as a unifying framework for this research (Moshagen et al., 2018). To date, little work has rigorously investigated whether the traits/constructs investigated in the context of the dark factor can be captured by existing models of normative personality, namely Agreeableness from the Five-factor Model. Thus, the "dark factor" may be an instance of the "jangle" fallacy, where two constructs with different names are in fact the same construct. METHOD We used a preregistered approach that made use of bass-ackwards factor analysis, structural equation modeling, and nomological network analysis to investigate the distinction between the D factor and Agreeableness. RESULTS Agreeableness and the D factor were similar in their coverage of antagonistic personality content, strongly negatively related (latent r = -.90), and showed near perfect profile dissimilarity (rICC = -.99). CONCLUSION The results suggested that the D factor can be understood as the opposite pole of Agreeableness (i.e., antagonism) and not as a distinct construct. We discuss the implications for researchers interested in continuing to advance the study of antagonistic personality traits.
Can Multi-Item Measures and Single-Item Measures be Trusted to Assess Self-Determination Theory Constructs in the Elderly?

BACKGROUND Measuring complex constructs, such as those from self-determination theory models, is challenging in the elderly due to the response process, particularly in collective data gathering. In order to examine this construct in physical activity settings we aimed at determining whether single-item measures were as good as pre-existing multi-item measures. For that reason, we developed seven single-item measures targeting perceived interpersonal styles, basic needs satisfaction, and well-being. METHOD We gathered evidence of validity and reliability for multi-item measures and single-item measures based on a sample of 128 elderly adults. Another sample of 62 elderly adults provided test-retest reliability for the single-item measures. RESULTS Favourable evidence of the expected internal structure, concurrent validity, and reliability was obtained for competence- and relatedness-supportive styles, and for satisfaction of the basic psychological need of relatedness, fairly good evidence was obtained for subjective vitality, whereas mixed evidence was obtained for autonomy-supportive style and the satisfaction of the basic psychological needs of autonomy and competence. CONCLUSIONS Single-item measures proved to be psychometrically sound substitutes for their multi-item counterparts, but the autonomy constructs need to be reconsidered. Furthermore the response process and consequences of testing should play a prominent role when devising questionnaires for the elderly.
A computational and bioinformatic analysis of ACE2: an elucidation of its dual role in COVID-19 pathology and finding its associated partners as potential therapeutic targets

Abstract Despite the continued global spread of the current COVID-19 pandemic, the nonavailability of a vaccine or targeted drug against this disease is still prevailing. The most established mechanism of viral entry into the body is considered to be via angiotensin-converting enzyme 2 (ACE2) acting as a receptor for viral spike protein thereby facilitating its entry in the cell. However, ACE2 is also involved in providing the protection from severe pathological changes. This article provides a computational and bioinformatics-based analysis of ACE2 with an objective of providing further insight into the earnest efforts to determine its true position in COVID-19 pathology. The results of this study show that ACE2 has strikingly low expression in healthy human lung tissue and was absent from the list of differentially expressed genes. However, when transcription factors were analyzed, we found a significant upregulation of FOS and downregulation of FOXO4 and FOXP2. Moreover, the miRNA prediction analysis revealed that miR-1246, whose upregulation has been experimentally established to be a cause of acute respiratory distress syndrome (ARDS), was found to be targeting the coding DNA sequence (CDS) of ACE2. This study presents a wide range of potentially important transcription factors as well as miRNA targets associated with ACE2 which can be potentially used for drug designing amid this challenging pandemic situation. Communicated by Ramaswamy H. Sarma
Comparison of the pattern of activation of the masticatory muscles among individuals with and without TMD: A systematic review

ABSTRACT Objective To verify the characteristics of the electromyographic signal of masticatory muscles in individuals with TMD and asymptomatic individuals. Methods A systematic review was carried out, selecting cross-sectional studies with at least one electromyographic parameter (FM; iEMG; RMS) of the masticatory muscles (anterior temporal, bilateral masseter, and suprahyoid muscles) of individuals with TMD compared to asymptomatic individuals. Results This review identified 1656 titles, of which 30 articles were included in the final analysis. It was observed that the data referring to the pattern of activation of masticatory muscles in the rest, isometry, and isotonic conditions of individuals with TMD are heterogeneous. This fact may be related to variability in the methods and analyses applied in the studies. Conclusion Data referring to the pattern of activation of masticatory muscles in the rest and isometry conditions and in the mastication cycle of individuals with TMD and asymptomatic individuals are diverse.
Public Concerns and Mental Health Changes Related to the COVID-19 Pandemic Lockdown in Saudi Arabia.

BACKGROUND Public measures to confine the spread of the novel coronavirus disease 2019 (COVID-19) infection involves partial or full lockdown by some countries including Saudi Arabia. Social isolation, and financial insecurity are potential risk factors for mental changes. This study aimed to address public concerns, and assess mental health changes, and the factors associated with mental health burden in response to the COVID-19 outbreak in Saudi Arabia after the full lockdown is widely employed. METHODS This cross-sectional study was conducted between 30th of April, and 10th of May, 2020 by posting an online survey on social media platforms (WhatsApp, and Twitter) to collect data on participants' demographics, concerns and worries related to the COVID-19 pandemic, and mental health changes using a validated Arabic version of the self-rated Hospital Anxiety and Depression Scale (HADS). RESULTS A total of 1,921 responded to the questionnaire. Of them, 1,429 (74.5%) were ≤ 45 years old, and 967 (50.3%) were males. Reported public concerns included disturbed lifestyle, getting self or family member infected, loss job or part of income, difficult access to routine health care, and 55.8% reported negative impact on their mental health. Hospital anxiety and depression scale revealed high rates of depression [717 (37.3%)], and anxiety [508 (26.4%)]. Binary logistic regression revealed that female gender, working for the private sector, smokers, and people with chronic diseases were at increased risk of mental illnesses (p < 0.05). CONCLUSIONS This study addressed serious public concerns, and substantially high rates of depression and anxiety related to the COVID-19 pandemic, and lockdown.
The capsules of Drosanthemum Schwantes (Ruschioideae, Aizoaceae)

Summary: Based on studies in more than 700 populations of Drosanthemum, the capsule morphology is described, and thirteen fruit types are distinguished. For 66 of the 108 accepted species, coloured figures of the capsules are presented. All names ever published in Drosanthemum are given and D. deciduum is described as a new species, D. subspinosum as a new combination; for many species, lectotypifications are presented.
Longitudinal retinal layer changes in preclinical Alzheimer’s disease

Several studies found reduced retinal thickness on optical coherence tomography (OCT) in Alzheimer’s disease (AD), even in preclinical stages, labelling this technique of interest as biomarker. In this study, we examine retinal thickness changes in preclinical AD, as defined by cognitively normal individuals with amyloid‐beta (Aβ) on positron emission tomography (PET).
Distribution and ecology of Colpomenia peregrina (Phaeophyceae) within the Northwest Atlantic

Abstract The saccate Asiatic brown seaweed Colpomenia peregrina was first recorded within the NW Atlantic in 1960 at Atkins Point, Nova Scotia. By 2014 it was recorded from 57 sites ranging from the Grand Barachois Lagoon, Newfoundland to South Wellfleet, MA. We first found extensive populations in New England at Fort Foster, Kittery, Maine where we subsequently conducted floristic and ecological studies of the alga. Colpomenia peregrina is superficially similar to another saccate brown alga, Leathesia marina, although they have differences in morphology, anatomy, and reproduction. The species is a common epiphyte on at least 33 seaweeds within the NW Atlantic, with Corallina officinalis being its most common host. It also grows on the seagrass Zostera marina and on at least two common invertebrates, Mytilus edulis and Semibalanus balanoides. Colpomenia peregrina is an annual somewhat like Petalonia fascia, with some specimens bearing plurilocular gametangia year-round. Based upon collection of samples from 57 sites, its convoluted hollow thalli had mean diameters and heights of less than 7.5 cm and damp dried weights of less than 10.0 g. Subtidal transects studies within the shallow subtidal zone at the Isles of Shoals, NH/ME, showed a wide range of densities (15–2721 m−2) and mean diameters of 0.47–1.72 cm. The demography of 663 drift, 442 attached, and 903 epiphytic thalli were documented from Fort Foster, ME during 31 monthly visits. Drift thalli had their largest diameter and height in winter (6.9×4.7 cm), whereas those from low intertidal ledge quadrats were largest in summer (3.4×2.5 cm), and random mid-intertidal pool samples were largest in the fall (2.9×1.6 cm). The mean number of epiphytic thalli on apical parts of three fucoid algae within a mid-intertidal pool ranged from 0.09 cm−1 on Ascophyllum nodosum, to 0.23 cm−1 on Fucus vesiculosus, and 0.43 cm−1 on F. distichus subsp. evanescens. The alga's southward expansion and colonization of low intertidal and shallow subtidal habitats in the NW Atlantic indicate that it is already competing for space and resources with native vegetation as well as causing removal of attached hosts.
Short communication Comparison of hCMV immediate early and CaMV 35S promoters in both plant and human cells

Cauliflower mosaic virus 35S promoter, widely used in transgenic crop plants, is known to be recognized in widely differing kinds of cells. Its activity in human cells may have impact on the risk assessment for the environmental release of genetically modified plants. In this study, transient expression of several constructs containing b-glucuronidase (GUS) gene driven by cauliflower mosaic virus 35S promoter or by immediate early promoter of human cytomegalovirus (pCMV) was tested in both potato leaf protoplasts and cultured human cells. The results showed very low but measurable activity of 35S promoter in human 293T-cells (0.01% of that revealed when using pCMV) and in 293 cells that do not produce SV40 T antigen this activity was even lower. On the other hand, in potato protoplasts, pCMV displayed nearly 1% activity seen with p35S. # 2003 Elsevier B.V. All rights reserved.
Mindfulness-Based Cognitive Therapy for Spanish Oncology Patients: The Bartley Protocol.

OBJECTIVES The aim of this study is to analyse whether MBCT will reduce the general level of psychopathology, increase the quality of life, and increase meta-knowledge about their emotional state in Spanish participants with cancer. METHOD The sample consisted of n = 88 Spanish oncology patients. This was a non-randomized, two-group (experimental vs waiting list) trial conducted in a naturalistic setting. We evaluated psychological distress (BSI-18), quality of life (FACT-G), and meta-knowledge of emotions (TMMS-24). RESULTS The participants who received the MBCT treatment improved more than the control group in distress (F= 6.79; p = .01, BSI-18), depression (F= 8.38; p = .005 ), quality of life -physical health (F = 5.56; p = .02), emotional state (F = 7.06; p= .01), and functional capacity (F = 7.98; p = .006), as well as meta-knowledge about their emotional state (F = 35.4; p = .01), and its subscales of perception, (F = 8.95; p = .004), comprehension, (F= 16.06; p = .01), and repair (F = 15.67; p = .01). CONCLUSIONS The Bartley MBCT program was feasible and showed promise in improving general psychopathology (depression), improving patients’ quality of life, and increasing meta-knowledge about their emotional state.
MiR‐133a acts as a tumor suppressor in lung cancer progression by regulating the LASP1 and TGF‐β/Smad3 signaling pathway

MiR‐133a has been confirmed to be involved in the development of multiple cancers including non‐small cell lung cancer (NSCLC). However, the precise molecular mechanism has not yet been fully elucidated. The purpose of this study was to investigate the functional role and underlying mechanism of miR‐133a in the progression of NSCLC.
Comparative study of phytochemical profiles and In Vitro multienzymes protein digestibility of two species of Mallotus subulatus --- A tropical underutilized hard-to-cook legume

Two varieties of Mallotus subulatus (white and brown), underutilized hard-to-cook legumes, were analysed for the nutrients, phytochemicals and in vitro multienzyme protein digestibility. The ash content of 4.38% and 4.25% were recorded for the white and brown varieties respectively. The two varieties are poor sources of fat. The protein content of the white variety (20.97%) was lower than that of brown variety (21.90). The crude fibre content of 9.68% for the white variety was significantly higher (p< 0.05) than 3.93% recorded for the brown variety. The two varieties of M. subulatus are good sources of Ca, K, P, Zn, Mg. Although, the quantity of non-heam Fe was low. The phytic acid and trypsin inhibitor --- 71.31mg/g and 34.37mg/g, respectively were higher in white variety than brown variety – 69.53mg/g and 30.44mg/g. The concentration of tannin in white and brown varieties were 28.10mg/g and 38.87mg/g, respectively. The percentage digestibility of the white variety was higher than that of the brown variety. The study showed that both varieties are good sources of critically important nutrients such as protein and their adaptation will not only strengthen dietary diversity but solve the wider problem of protein energy malnutrition (PEM) — a major nutritional syndrome in many parts of the world.