Miracle-dz/UniFAR-FTUs huggingface dataset:
Adipose‐Derived Stromal Vascular Fraction and Cultured Stromal Cells as Trophic Mediators for Tendon Healing

Adipose‐derived stromal vascular fraction (SVF) is a heterogeneous population of cells that yields a homogeneous population of plastic‐adherent adipose tissue‐derived stromal cells (ASC) when culture‐expanded. SVF and ASC have been used clinically to improve tendon healing, yet their mechanism of action is not fully elucidated. The objective of this study was to investigate the potential for ASC to act as trophic mediators for tendon healing. Flexor digitorum superficialis tendons and adipose tissue were harvested from adult horses to obtain SVF, ASC, and tenocytes. Growth factor gene expression was quantified in SVF and ASC in serial passages and growth factors were quantified in ASC‐conditioned medium (CM). Microchemotaxis assays were performed using ASC‐CM. Tenocytes were grown in co‐culture with autologous ASC or allogeneic SVF. Gene expression for insulin‐like growth factor 1 (IGF‐1), stromal cell‐derived factor‐1α (SDF‐1α), transforming growth factor‐β1 (TGF‐β1) and TGF‐β3 was significantly higher in SVF compared to ASC. Concentrations were significantly increased in ASC‐CM compared to controls for IGF‐1 (4‐fold) and SDF‐1α (6‐fold). Medium conditioned by ASC induced significant cell migration in a dose‐dependent manner. Gene expression for collagen types I and III, decorin, and cartilage oligomeric matrix protein was modestly, but significantly increased following co‐culture of tenocytes with autologous ASC. Our findings support the ability of SVF and ASC to act as trophic mediators in tendon healing, particularly through chemotaxis, which stands to critically impact the intrinsic healing response. In vivo studies to further delineate the potential for SVF and/or ASC to improve tendon healing are warranted. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1429–1439, 2019.
Inadequate Zinc Intake in India: Past, Present, and Future

Background: India has made important strides in reducing nutritional deficiencies over the past several decades. However, for micronutrients such as zinc, previous studies have suggested a worsening situation, contrary to most other dietary indicators. Adding to this burden, higher carbon dioxide (CO2) levels of 550 ppm, projected to potentially occur within decades, could reduce the zinc content of many staple crops. Objective: To assess the historical prevalence of inadequate zinc intake, as well as to estimate the future prevalence attributable to rising CO2. Methods: Seven household food consumption surveys between 1983 and 2012 were used to calculate total dietary zinc, phytate, and absorbable zinc intakes and to assess the prevalence of historic inadequacy in zinc intake. The added nutritional effect of elevated CO2 on zinc intake is then modeled. Results: Prevalence of inadequate absorbable zinc intake has increased from 17.1% (15.3%-19.0%) in 1983 to 24.6% (22.3%-27.1%) in 2011-12, corresponding to an additional 82 million people consuming inadequate zinc than would have otherwise if 1983 rates had persisted. These increases in inadequacy have been driven by a relatively constant zinc intake being increasingly insufficient to meet a 5% growth in zinc requirements due to the aging of the population. Reaching 550 ppm CO2 by 2050 could potentially increase the prevalence of inadequate zinc intake by another 3.9 percentage points (2.1-5.8), corresponding to 65 million additional people having inadequate zinc intake. Conclusions: The persistently worsening trend for zinc—opposite most other measures of human nutrition—shows that it may pose an ongoing risk unless addressed.
Anthropometric factors and risk of myeloid leukaemias and myelodysplastic syndromes: a prospective study and meta‐analysis

There is insufficient evidence linking excess body weight to risk of myeloid malignancies. We investigated this association using data from the Cancer Prevention Study‐II (CPS‐II), and a meta‐analysis of published cohort studies. Among 152 090 CPS‐II participants, 387 acute myeloid leukaemias (AML), 100 chronic myeloid leukaemias (CML) and 170 MDS were identified over 21 years of follow‐up. In CPS‐II, body mass index (BMI) was weakly associated with risk of CML (hazard ratio [HR] = 1·04, 95% confidence interval [CI]: 0·99–1·09 per 1 unit increase in BMI), AML (HR = 1·01, 95% CI: 0·98–1·03) and MDS (HR = 1·03, 95% CI: 0·99–1·07). After controlling for other anthropometric factors, no clear association was observed for height, BMI at age 18 years or weight change. In the meta‐analysis (n = 7117 myeloid leukaemias), BMI 25–29·9 kg/m2 (HRpooled = 1·36, 95% CI: 1·12–1·59) and BMI ≥30 kg/m2 (HRpooled = 1·43, 95% CI: 1·18–1·69) were associated with higher risk of myeloid leukaemia overall, compared to a BMI <25 kg/m2. Likewise, BMI ≥25 kg/m2 was positively associated with both AML and CML risk individually in the meta‐analysis. These results underscore the need for large studies to detect associations with rare cancers, and show a modest, but positive association between excess body weight and myeloid malignancy risk.
A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding‐based biomonitoring

This study summarizes results of a DNA barcoding campaign on German Diptera, involving analysis of 45,040 specimens. The resultant DNA barcode library includes records for 2,453 named species comprising a total of 5,200 barcode index numbers (BINs), including 2,700 COI haplotype clusters without species‐level assignment, so called “dark taxa. ” Overall, 88 out of 117 families (75%) recorded from Germany were covered, representing more than 50% of the 9,544 known species of German Diptera. Until now, most of these families, especially the most diverse, have been taxonomically inaccessible. By contrast, within a few years this study provided an intermediate taxonomic system for half of the German Dipteran fauna, which will provide a useful foundation for subsequent detailed, integrative taxonomic studies. Using DNA extracts derived from bulk collections made by Malaise traps, we further demonstrate that species delineation using BINs and operational taxonomic units (OTUs) constitutes an effective method for biodiversity studies using DNA metabarcoding. As the reference libraries continue to grow, and gaps in the species catalogue are filled, BIN lists assembled by metabarcoding will provide greater taxonomic resolution. The present study has three main goals: (a) to provide a DNA barcode library for 5,200 BINs of Diptera; (b) to demonstrate, based on the example of bulk extractions from a Malaise trap experiment, that DNA barcode clusters, labelled with globally unique identifiers (such as OTUs and/or BINs), provide a pragmatic, accurate solution to the “taxonomic impediment”; and (c) to demonstrate that interim names based on BINs and OTUs obtained through metabarcoding provide an effective method for studies on species‐rich groups that are usually neglected in biodiversity research projects because of their unresolved taxonomy.
ANTIFUNGAL DISINFECTANTS EFFICIENCY ON ASPERGILLUS STRAINS FROM CAMEL’S MILK AND DRINKING WATER: BIOLOGICAL DETOXIFICATION OF AFLATOXIN-M1

This study was conducted to assess the distribution of mold and mycotoxins in raw camel’s milk and water samples and to evaluate the fungicidal activity of three disinfectants and the detoxification capabilities of LAB towards aflatoxin M1 in camel’s milk. Molds were isolated from 56 and 64% of water and raw camel milk samples, respectively. Aspergillus spp. were the most abundant species isolated from water and raw camel milk samples with 10.9 and 11.6%, respectively, followed by Penicillium and Fusarium spp. The concentrations of aflatoxin-B1 (AFB1) and ochratoxin-A (OTA) in the examined water samples were 7.97 and 8.28 μg/l, respectively. Furthermore, the concentrations of aflatoxin-M1 (AFM1) and ochratoxin-A (OTA) in the examined raw camel milk samples were 1.89 and 1.69 μg/l, respectively. Quaternary ammonium/glutaraldehyde based disinfectant was the most efficient disinfectants against the isolated fungal species. On contaminated dairy surfaces; exposure of A. flavus to Quaternary ammonium/glutaraldehyde based disinfectant for shorter contact times less than one-hour exhibited reduced efficacy with log reduction not exceed 2.6 log, while for A. ochraceus, a minimum of 15 minutes’ exposure time was required to induce good efficacy with 3.2 log reduction. Adjusting exposures time for one hour showed good efficacy for both A. flavus and A. ochraceus with 4.5 and 4.6 log reduction, respectively. The AFM1 concentration in prepared yoghurt from camel's milk was reduced to 24.6% after the first 5 days of incubation; and after incubation for 15 and 20 days, further reduction to 45.9 and 55.7% was observed, respectively.
Older and younger adults’ interactions with friends and strangers in an iterated prisoner’s dilemma

ABSTRACT This study examined age-related differences in behavioral reactions to interpersonal conflict within an iterated prisoner’s dilemma (PD). Participants completed an iterated PD game alone and with a partner, either a stranger or a friend who accompanied them to the session. The partner, however, was actually a program that occasionally behaved selfishly or always reciprocated. Afterwards, participants formed trait impressions of their partner’s morality and competence. Participants cooperated more with friends than strangers and more with reciprocating partners than selfish ones. Older adults cooperated more with selfish partners and offered more favorable impressions than did younger adults. Overall, perceived partner trait morality was positively associated with cooperative behavior. Relative to younger adults, older adults were more passive during conflict but grew less so as selfishness continued. This passivity co-occurred with more favorable partner impressions and better objective performance, suggesting a degree of calibration not shown by younger adults.
Extracellular Proteolytic Activity and Amino Acid Production by Lactic Acid Bacteria Isolated from Malaysian Foods

Amino acids (AAs) are vital elements for growth, reproduction, and maintenance of organisms. Current technology uses genetically engineered microorganisms for AAs production, which has urged the search for a safer food-grade AA producer strain. The extracellular proteolytic activities of lactic acid bacteria (LAB) can be a vital tool to hydrolyze extracellular protein molecules into free AAs, thereby exhibiting great potential for functional AA production. In this study, eight LAB isolated from Malaysian foods were determined for their extracellular proteolytic activities and their capability of producing AAs. All studied LAB exhibited versatile extracellular proteolytic activities from acidic to alkaline pH conditions. In comparison, Pediococcus pentosaceus UP-2 exhibited the highest ability to produce 15 AAs extracellularly, including aspartate, lysine, methionine, threonine, isoleucine, glutamate, proline, alanine, valine, leucine, tryptophan, tyrosine, serine, glycine, and cystine, followed by Pediococcus pentosaceus UL-2, Pediococcus acidilactici UB-6, and Pediococcus acidilactici UP-1 with 11 to 12 different AAs production detected extracellularly. Pediococcus pentosaceus UL-6 demonstrated the highest increment of proline production at 24 h of incubation. However, Pediococcus acidilactici UL-3 and Lactobacillus plantarum I-UL4 exhibited the greatest requirement for AA. The results of this study showed that different LAB possess different extracellular proteolytic activities and potentials as extracellular AA producers.
The Role of Acid Suppressants in the Prevention of Anticoagulant-Related Gastrointestinal Bleeding: A Systematic Review and Meta-Analysis

Background/Aims Although acid suppressants are widely used for the prevention or treatment of drug-induced upper gastrointestinal bleeding (GIB), evidence regarding the prevention of anticoagulant-related GIB is scarce. The aim of this study was to evaluate the protective effect of acid suppressants against anticoagulant-related GIB. Methods A systematic review was conducted of studies that evaluated the protective effect of acid suppressants against anticoagulant-related GIB found in PubMed, the Cochrane library, Embase, and KoreaMed from the date of database inception to April 2018. Random effect model meta-analyses with sensitivity analyses were conducted. The methodological quality of each included publication was evaluated using the Risk of Bias Assessment Tool for Nonrandomized Studies. Publication bias was assessed. Results In total, six nested case-control or cohort studies were identified and analyzed. Proton-pump inhibitors (PPI) had a protective effect against upper GIB in patients on dicumarinics (risk ratio [RR], 0.56; 95% confidence interval [CI], 0.38 to 0.83; I2, 0%); however, the histamine-2 receptor antagonist did not have the same effect (RR, 0.97; 95% CI, 0.52 to 1.81; I2, 0%). Acid suppressants did not have a protective effect against GIB in patients on dabigatran (hazard ratio, 0.78; 95% CI, 0.44 to 1.37; I2, 81.8%). Conclusions The protective effect of PPIs against dicumarinics-related upper GIB was clear, while there was no evidence supporting the protective effect of acid suppressants against dabigatran-related GIB. However, in the absence of randomized trials demonstrating a lack of bias, solid conclusions cannot be drawn.
Assessment of Toxic Effects of Ochratoxin A in Human Embryonic Stem Cells

Ochratoxin A (OTA) is a mycotoxin produced by different Aspergillus and Penicillium species, and it is considered a common contaminant in food and animal feed worldwide. On the other hand, human embryonic stem cells (hESCs) have been suggested as a valuable model for evaluating drug embryotoxicity. In this study, we have evaluated potentially toxic effects of OTA in hESCs. By using in vitro culture techniques, specific cellular markers, and molecular biology procedures, we found that OTA produces mild cytotoxic effects in hESCs by inhibiting cell attachment, survival, and proliferation in a dose-dependent manner. Thus, we suggest that hESCs provide a valuable human and cellular model for toxicological studies regarding preimplantation stage of human fetal development.
Time-dependence of NIHSS in predicting functional outcome of patients with acute ischemic stroke treated with intravenous thrombolysis

Objectives The National Institute of Health Stroke Scale (NIHSS) is a predictor for the prognosis of acute ischaemic stroke (AIS) and its prediction is time-dependent. We examined the performance of NIHSS at different timepoints in predicting functional outcome of patients with thrombolysed AIS. Methods This prospective study included 269 patients with AIS treated with recombinant tissue plasminogen activator (rt-PA). Unfavourable functional outcome was defined as modified Rankin Scale score 4–6 at 3 months after rt-PA treatment. Receiver operating characteristic curves were used to examine the predictive power of NIHSS score at admission and 2 hours/24 hours/7 days/10 days after rt-PA treatment. Youden’s index was used to select the threshold of NIHSS score. Logistic regression was used to estimate the ORs of unfavourable functional outcome for patients with NIHSS score higher than the selected thresholds. Results The threshold of NIHSS score at admission was 12 (sensitivity: 0.51, specificity: 0.84) with an acceptable predictive power (area under curve [AUC] 0.74) for unfavourable functional outcome. The threshold changed to 5 at 24 hours after rt-PA treatment (sensitivity: 0.83, specificity: 0.65) and remained unchanged afterwards. The predictive power and sensitivity sequentially increased over time and peaked at 10 days after rt-PA treatment (AUC 0.92, sensitivity: 0.85, specificity: 0.80). NIHSS scores higher than the thresholds were associated with elevated risk of unfavourable functional outcome at all timepoints (all p<0.001). Conclusions NIHSS is time-dependent in predicting AIS prognosis with increasing predictive power over time. Since patients whose NIHSS score ≥ 12 are likely to have unfavourable functional outcome with rt-PA treatment only, mechanical thrombectomy should be largely taken into consideration for these patients.
Development and Experimental Validation of Real Fluid Models for CFD Calculation of ORC and Steam Turbine Flows

The article describes an interpolation–analytical method of reconstruction of the IAPWS-95 equations of state and the modified Benedict–Webb–Rubin equations of state with 32 terms (mBWR32). The method enables us to provide the thermodynamic closure in 3D computational fluid dynamics (CFD) calculations of turbomachinery flows with real working media, such as steam and Organic Rankine Cycle (ORC) fluids. The described approach allows for the sufficient accuracy of 3D flow calculations and does not require a significant increase in computational cost over perfect gas calculations. The method is validated against experimental data from measurements and compared with computational results from the model using the Tammann equation of state. Three turbine blading systems are considered—a multi-stage configuration from a low-pressure cylinder of a large-power steam turbine and two ORC microturbines working with organic media HFE7100 and R227ea. The calculation results obtained using the described method of approximation of the IAPWS-95 and mBWR32 equations exhibit satisfactory agreement with the experimental data, considering pressures, temperatures and enthalpies in key sections, as well as turbine power and efficiency in a wide range of changing thermodynamic parameters. In contrast, the Tammann equation of state provides acceptable results only for relatively small changes of thermodynamic parameters.
Renal acid excretion contributes to acid–base regulation during hypercapnia in air-exposed swamp eel (Monopterus albus)

ABSTRACT The swamp eel (Monopterus albus) uses its buccal cavity to air breathe, while the gills are strongly reduced. It burrows into mud during the dry season, is highly tolerant of air exposure, and experiences severe hypoxia both in its natural habitat and in aquaculture. To study the ability of M. albus to compensate for respiratory acidosis, we implanted catheters to sample both arterial blood and urine during hypercapnia (4% CO2) in either water or air, or during whole-animal air exposure. These hypercapnic challenges caused an immediate reduction in arterial pH, followed by progressive compensation through a marked elevation of plasma HCO3− over the course of 72 h. There was no appreciable rise in urinary acid excretion in fish exposed to hypercapnia in water, although urine pH was reduced and ammonia excretion did increase. In the air-exposed fish, however, hypercapnia was attended by a large elevation of ammonia in the urine and a large rise in titratable acid excretion. The time course of the increased renal acid excretion overlapped with the time period required to elevate plasma HCO3−, and we estimate that the renal compensation contributed significantly to whole-body acid–base compensation. Summary: The swamp eel – an obligate air-breathing fish from South East Asia – can completely restore extracellular pH within days of exposure to hypercapnia and the kidneys play an important role in this response.
High Optical Response of Niobium-Doped WSe2-Layered Crystals

The optical properties of WSe2-layered crystals doped with 0.5% niobium (Nb) grown by the chemical vapor transport method were characterized by piezoreflectance (PzR), photoconductivity (PC) spectroscopy, frequency-dependent photocurrent, and time-resolved photoresponse. With the incorporation of 0.5% Nb, the WSe2 crystal showed slight blue shifts in the near band edge excitonic transitions and exhibited strongly enhanced photoresponsivity. Frequency-dependent photocurrent and time-resolved photoresponse were measured to explore the kinetic decay processes of carriers. Our results show the potential application of layered crystals for photodetection devices based on Nb-doped WSe2-layered crystals.
“I’m Trying to Give Them My Face.” Everyday Embodied Agency of the Muslim Other in Amsterdam

Focusing on the Muslim Other in Amsterdam, the article explores how individual young Muslims embody their agency in the forms of micro-practices and socio-spatial relations. By highlighting the spatio-corporeal aspects of Muslims’ presence within urban space, our ethnography foregrounds how young Muslims negotiate identities and belonging within everyday urban spaces of encounter. Dealing with the everyday panoptical white Othering gaze, we describe a range of “unremarkable” ordinary practices and everyday small agencies that young Muslims in Amsterdam adopt including invisibility, challenging whiteness, and accumulating whiteness.
Characterization of intentional-abuse venlafaxine exposures reported to poison control centers in the United States

ABSTRACT Background: Venlafaxine use to achieve an amphetamine-like high has been described but data regarding the epidemiology and clinical effects are sparse. Objectives: Describe the prevalence and toxicity of venlafaxine abuse reported to US poison control centers. Methods: This was a retrospective review of venlafaxine exposures reported to the National Poison Data System (NPDS) from 2000 to 2016. Inclusion criteria were: age 12 years and older, reason for exposure intentional-abuse, and either single-substance exposure or venlafaxine was the first substance. The primary outcome was prevalence of intentional-abuse of venlafaxine. Secondary outcomes characterized demographics, geographic distribution, toxicity, and outcomes. Results: Intentional-abuse accounted for 752 of 85,621 venlafaxine exposures. Overall prevalence was 87.8 intentional-abuse exposures/10,000 venlafaxine exposures reported to NPDS (range, 59.3–117.6/10,000). Prevalence decreased from 107/10,000 in 2000 to 59.3/10,000 in 2016. Median age was 23 years and 50% were female. Primary route was ingestion (90.8%) with 4.7% using venlafaxine via inhalation/intranasal insufflation, and 3.7% both routes. There were 227 venlafaxine-only exposures; 54.0% were treated/released from the emergency department, 20% were admitted for medical management, 9.0% to a psychiatric facility, and 17.0% managed at home. Known medical outcomes for single-substance exposures were: no effect (24.0%), minor (39.0%), moderate (33.0%), and major (4.0%); no deaths occurred. Most frequent clinical effects were tachycardia (33.9%), drowsiness (20.7%), and agitation (11.5%). Conclusion: The prevalence of venlafaxine abuse reported to poison control centers has decreased. Medical outcomes are usually not serious. Clinicians should be aware that non-medical use is possible but infrequently reported to poison control centers.