Miracle-dz/UniFAR-FTUs huggingface dataset:
De novo genome assembly of Bradysia cellarum (Diptera: Sciaridae), a notorious pest in traditional special vegetables in China

Bradysia cellarum (Diptera: Sciaridae) is a destructive vegetable insect pest infesting more than 30 species of host plants from seven families in Asia and Europe. B. cellarum causes grave problems in Chinese chive, which originated in China and is cultivated widely in East Asia. The B. cellarum infestation results in economic losses and subsequent severe food safety problems in farm productions, insecticide resistance and environmental pollution. The genomic and molecular information of B. cellarum to delineate the biological features, insecticide resistance, evolution remains poorly understood. Herein, we decode the whole genome of B. cellarum to delineate the underlying molecular mechanisms causing insecticide resistance. We constructed a highly reliable genome for B. cellarum using PacBio, Illumina and 10X Genomics sequencing platforms. The genome size of B. cellarum was 375.91 Mb with a contig N50 of 1.57 Mb. A total of 16,231 genes were identified, among which 93.8% were functionally annotated, and 42.06% were repeat sequences. According to phylogenetic analysis, B. cellarum diverged from the common ancestor of Drosophila melanogaster and Musca domestica ~139.3–191.0 million years ago. Moreover, some important genes responsible for significant insecticide resistance, such as cytochrome P450s, ABC transporters and those involved in glutathione metabolism, were expanded in B. cellarum. We assembled a high‐quality B. cellarum genome to provide valuable insights into their life history strategies, insecticide resistance and biological behaviours. It also lays the foundation for exploring gene structure and functional evolution, as well as comparative genomics of B. cellarum and other model insect species.
Erythroid side scatter: A parameter that improves diagnostic accuracy of flow cytometry myelodysplastic syndrome scoring

Background: Flow cytometry immunophenotyping (FCM) is a benchmark test for integrated diagnosis of myelodysplastic syndromes (MDS). Our department's FCM‐MDS‐score follows international guidelines and additionally includes the maturing erythroid (mEry) side scatter (SSC)/lymphocyte SSC ratio (mErySSCr), often increased in MDS patients. A recent exploratory computational flow analysis study highlighted mErySSC as the top feature for separating MDS from non‐MDS. Thus, we sought to systematically evaluate the diagnostic accuracy of mErySSCr in conventional diagnostic FCM as used currently in‐house.
The effect of board composition on financial performance of commercial banks in Kenya

This research study was undertaken with the objective to establish the effect of board composition on financial performance of commercial banks in Kenya. The study covered the period 2013 to 2015. Board composition was measured by board size, gender diversity and board independence; while financial performance was measured by the return to assets ratio. The study used a descriptive research design. The population of the study comprised all the forty two commercial banks in Kenya over the period of study. The study was therefore a census study. The research study collected secondary data from the annual reports published by the said firms as well as their financial statements. The study achieved a response rate of 59.52% as only twenty five banks had complete data set sought. Data was obtained and organized and presented in form of tables. Data analysis was then undertaken using descriptive statistics, correlation and regression analyses, and conclusions drawn. The researcher finds that overall board composition has a positive effect on financial performance of commercial banks in Kenya. Board size and board independence have positive effect on return on assets (the proxy for financial performance), while gender diversity has a negative effect. The researcher concludes therefore that banks management and owners should properly constitute their boards, as this affects financial performance. The researcher recommends further research on the gender diversity variable.
Smart Shape‐Memory Polymeric String for the Contraction of Blood Vessels in Fetal Surgery of Sacrococcygeal Teratoma

Shape‐memory polymers (SMPs) are promising materials in numerous emerging biomedical applications owing to their unique shape‐memory characteristics. However, simultaneous realization of high strength, toughness, stretchability while maintaining high shape fixity (Rf) and shape recovery ratio (Rr) remains a challenge that hinders their practical applications. Herein, a novel shape‐memory polymeric string (SMP string) that is ultra‐stretchable (up to 1570%), strong (up to 345 MPa), tough (up to 237.9 MJ m−3), and highly recoverable (Rf averagely above 99.5%, Rr averagely above 99.1%) through a facile approach fabricated solely by tetra‐branched poly(ε‐caprolactone) (PCL) is reported. Notably, the shape‐memory contraction force (up to 7.97 N) of this SMP string is customizable with the manipulation of their energy storage capacity by adjusting the string thickness and stretchability. In addition, this SMP string displays a controllable shape‐memory response time and demonstrates excellent shape‐memory‐induced contraction effect against both rigid silicone tubes and porcine carotids. This novel SMP string is envisioned to be applied in the contraction of blood vessels and resolves the difficulties in the restriction of blood flow in minimally invasive surgeries such as fetoscopic surgery of sacrococcygeal teratoma (SCT).
Symptom Cluster Profiles Among Adults with Insomnia and Heart Failure

ABSTRACT Objective/Background Both heart failure (HF) and insomnia are associated with high symptom burden that may be manifested in clustered symptoms. To date, studies of insomnia have focused only on its association with single symptoms. The purposes of this study were to: (1) describe daytime symptom cluster profiles in adults with insomnia and chronic HF; and (2) determine the associations between demographic and clinical characteristics, insomnia and sleep characteristics and membership in symptom cluster profiles. Participants One hundred and ninety-five participants [M age 63.0 (SD12.8); 84 (43.1%) male; 148 (75.9%) New York Heart Association Class I/II] from the HeartSleep study (NCT0266038), a randomized controlled trial of the sustained effects of cognitive behavioral therapy for insomnia (CBT-I). Methods We analyzed baseline data, including daytime symptoms (fatigue, pain, anxiety, depression, dyspnea, sleepiness) and insomnia (Insomnia Severity Index), and sleep characteristics (Pittsburgh Sleep Quality Index, wrist actigraphy). We conducted latent class analysis to identify symptom cluster profiles, bivariate associations, and multinomial regression. Results We identified three daytime symptom cluster profiles, physical (N = 73 participants; 37.4%), emotional (N = 12; 5.6%), and all-high symptoms (N = 111; 56.4%). Body mass index, beta blockers, and insomnia severity were independently associated with membership in the all-high symptom profile, compared with the other symptom profile groups. Conclusions Higher symptom burden is associated with more severe insomnia in people with stable HF. There is a need to understand whether treatment of insomnia improves symptom burden as reflected in transition from symptom cluster profiles reflecting higher to lower symptom burden.
The response of Military Lower Extremity and Hybrid III leg using the Hybrid III and EuroSID-2 ATD in vertical loading impacts

Abstract Objective Antivehicular landmines (AVLs) and underbelly improvised explosive devices (IEDs) are found to be some of the major threats for military vehicles and their occupants. Anthropomorphic test devices (ATDs) such as the 50th percentile Hybrid III (HIII) and EuroSID2-re (ES2-re) are used to assess injury caused by AVLs or IEDs in order to develop mitigation strategies by analyzing lower leg data in tibia load cells. Methods This article presents the evaluation of the injury measurement response of the Hybrid III and ES2-re ATDs using both the HIII and Military Lower Extremity (MIL-LX) instrumented lower legs impacted by the Modified Lower Limb Impactor (MLLI). The MIL-LX leg tested with the HIII ATD measures higher forces than when tested with the ES2-re ATD. Results In general, the MIL-LX upper tibia load cell measures peak forces that are considerably lower than that measured by the HIII lower leg with both the HIII and ES2-re ATD. The HIII leg fails earlier with both the HIII and ES2-re ATD compared to the MIL-LX leg. The study shows that the HIII and MIL-LX lower leg are not equivalent in their assessment of protective capability of armored vehicles when either attached to the HIII or ES2-re ATD. Conclusions These results show the importance of selecting the correct lower leg surrogate during AVL or IED testing, which can lead to a pass or fail of the armored vehicle. These findings offer insight into the response of each surrogate lower leg with the different ATDs and can be used to develop new mitigation strategies.
Loss of ubiquitin-specific peptidase 18 destabilizes 14-3-3ζ protein and represses lung cancer metastasis

ABSTRACT Cancer metastasis is a major cause of cancer-related mortality. Strategies to reduce metastases are needed especially in lung cancer, the most common cause of cancer mortality. We previously reported increased ubiquitin-specific peptidase 18 (USP18) expression in lung and other cancers. Engineered reduction of USP18 expression repressed lung cancer growth and promoted apoptosis. This deubiquitinase (DUB) stabilized targeted proteins by removing the complex interferon-stimulated gene 15 (ISG15). This study explores if the loss of USP18 reduced lung cancer metastasis. USP18 knock-down in lung cancer cells was independently achieved using small hairpin RNAs (shRNAs) and small interfering RNAs (siRNAs). USP18 knock-down reduced lung cancer growth, wound-healing, migration, and invasion versus controls (P < .001) and markedly decreased murine lung cancer metastases (P < .001). Reverse Phase Protein Arrays (RPPAs) in shRNA knock-down lung cancer cells showed that 14-3-3ζ protein was regulated by loss of USP18. ISG15 complexed with 14-3-3ζ protein reducing its stability. Survival in lung adenocarcinomas (P < .0015) and other cancers was linked to elevated 14-3-3ζ expression as assessed by The Cancer Genome Atlas (TCGA). The findings were confirmed and extended using 14-3-3ζ immunohistochemical assays of human lung cancer arrays and syngeneic murine lung cancer metastasis models. A direct 14-3-3ζ role in controlling lung cancer metastasis came from engineered 14-3-3ζ knock-down in lung cancer cell lines and 14-3-3ζ rescue experiments that reversed migration and invasion inhibition. Findings presented here revealed that USP18 controlled metastasis by regulating 14-3-3ζ expression. These data provide a strong rationale for developing a USP18 inhibitor to combat metastases.
Fate and seasonality of antimicrobial resistance genes during full-scale anaerobic digestion of cattle manure across seven livestock production facilities.

Anaerobic digestion has been suggested as an intervention to attenuate antibiotic resistance genes (ARGs) in livestock manure but supporting data has typically been collected at laboratory scale. Few studies have quantified ARG fate during full-scale digestion of livestock manure. We sampled untreated manure and digestate from 7 full-scale mesophilic dairy manure digesters to assess ARG fate through each system. Samples were collected biweekly from December through August (i.e., winter, spring, and summer; n = 235 total) and analyzed by quantitative polymerase chain reaction for intI1, erm(B), sul1, tet(A), and tet(W). Concentrations of intI1, sul1, and tet(A) decreased during anaerobic digestion, but their removal was less extensive than expected based on previous laboratory studies. Removal for intI1 during anaerobic digestion equaled 0.28 ± 0.03 log10 units (mean ± standard error), equivalent to only 48% removal and notable given intI1's role in horizontal gene transfer and multiple resistance. Furthermore, tet(W) concentrations were unchanged during anaerobic digestion (p > 0.05), and erm(B) concentrations increased by 0.52 ± 0.03 log10 units (3.3-fold), which is important given erythromycin's status as a critically important antibiotic for human medicine. Seasonal log10 changes in intI1, sul1, and tet(A) concentrations were ≥ 50% of corresponding log10 removals by anaerobic digestion, and variation in ARG and intI1 concentrations among digesters was quantitatively comparable to anaerobic digestion effects. These results suggest that mesophilic anaerobic digestion may be limited as an intervention for ARGs in livestock manure and emphasize the need for multiple farm-level interventions to attenuate antibiotic resistance. This article is protected by copyright. All rights reserved.
Arteriovenous fistula in predialysis chronic kidney disease patients and rate of decline of glomerular filtration rate

Purpose: To quantify the evidence for the role of arteriovenous fistula (AVF) in predialysis CKD patients for the rate of decline of glomerular filtration rate (GFR). Background: Pre-emptive placement of arteriovenous fistula (AVF) in late-stage chronic kidney disease (CKD) patients is being advocated by all the major guidelines. Recent studies have suggested that pre-emptive AVF has a beneficial effect on glomerular filtration rate (GFR) also. Methods: We conducted a literature search to retrieve all published studies related to the effect of AVF on the rate of decline of GFR using the electronic databases Google Scholar, PubMed, Central, Cochrane Library, clinialtrial.gov. Screening of studies and data extraction were done according to the PRISMA guidelines. We used the NIH assessment tool for the methodological quality assessment of the included studies. Extracted data from the six studies were pooled and analyzed. Results: Six studies involving 3871 patients reported the effect of AVF creation on the rate of decline of GFR in late CKD patients. Evidence for statistically significant decline of eGFR after AVF creation compared to prior status (SMD −1.57, 95% CI −3.08 to −0.07, p < 0.001) was observed. Conclusion: Our meta-analysis observed preliminary evidence that the creation of AVF might have a potential added benefit in terms of estimated GFR improvement, though to a very small extent with a low level of certainty. More scientific data with high-quality studies are needed to substantiate this finding.
Exploring new-graduate physiotherapists’ preparedness for, and experiences working within, Australian acute hospital settings

ABSTRACT Background New-graduate physiotherapists experience a steep learning curve when transitioning from student to clinician. The acute hospital setting is known to present unique challenges for health clinicians, however, the preparedness of new-graduate physiotherapists for working within this setting remains unclear. Purpose The aim of this study was to investigate new-graduate physiotherapists’ experiences of working in acute hospital settings and their perceptions toward how their pre-professional training prepared them for this setting. Methods A qualitative study with a general inductive approach was used. Semi-structured interviews with new-graduate physiotherapists working in acute hospital settings were undertaken (n = 14). Interview data were subject to thematic analysis. Results Four themes were generated from the data: 1) multifactorial and high-pressure nature; 2) managing relationships; 3) realizing responsibility; and 4) constructing realistic experiences. Conclusion The acute hospital setting presents unique obstacles and additional challenges when transitioning from student to clinician. New-graduates value the role of pre-professional training in their preparation for this context, however, new-graduates reflected on being sheltered from some areas of practice as students. Recommendations are suggested for education providers to adapt pre-professional training, and for employers to implement workplace strategies, which may support new-graduate physiotherapists in the acute hospital setting.
Impact of the Act‐Belong‐Commit campaign on mental health help‐seeking behaviour

Abstract Issue addressed Despite the high prevalence of mental ill‐health amongst Australians, many people do not seek help for their mental ill‐health. A delay in help‐seeking is associated with poorer outcomes. This study investigated the extent to which the Act‐Belong‐Commit mental health promotion campaign prompted people to seek information or professional help for mental ill‐health. Methods A sample of 1200 respondents took part in two state‐wide surveys (n = 600 each). Participants aware of the Act‐Belong‐Commit campaign were asked questions related to information‐seeking and help‐seeking behaviours because of the campaign. Results Of those aware of the campaign, 8% stated that the campaign prompted them to seek information and 4% stated that the campaign prompted them to seek help for a mental health problem. Those with a mental illness experience (MIE) were significantly more likely than those without to report that the campaign prompted them to look for information (12% vs 6%) and seek help for a mental health problem (9.5% vs 1.2%). Extrapolating these results to the total adult population of Western Australia indicated that around 120 000 adults had sought mental health information, and around 60 000 had sought help as a result of the campaign. Conclusions The campaign not only initiated the seeking of information or professional help for a mental health problem amongst those with no prior thoughts of such, but also prompted those who were already thinking about seeking information or getting help to act sooner than they otherwise would. So what? Although previous research suggests that mental health literacy interventions have limited impact on help‐seeking, the presented data show that the Act‐Belong‐Commit approach can have a significant impact on help‐seeking, particularly amongst those with a MIE, which could yield substantial social and economic return on investment benefits if intensified at both the media and community grass roots levels.
Partnering and networks as a strategy to address de alio market entry challenges: the why, how and with whom to collaborate in the biotechnology industry

It is important to study companies finding, creating, and entering new markets, especially when experiencing pressure in their mature home markets. The strategy context of commercializing a new innovative product into an emergent market segment, in an unfamiliar industry (de alio), with completely different business logics is extremely demanding, original, and interesting. The target market (biotechnology) is ranked one of the most high-risk and complex, involving symbiotic partnering, deep science, regulation and learning race competition dynamics. On top of this, the multinational case company faces extra challenges of a newcomer, without enough knowledge, access or networks, with internal coordination of two different business logics, and the accelerating competition of the emergent market. These factors contrast with the company home industry (material manufacturing) which is more calculated and mature, and where the company enjoys the incumbent position. Thus, a target industry partner is important and the partnering issues of de alio companies merit attention. At the same time, globalization is changing industries by intensifying the importance of partnering. This study provides a snapshot update on how the trend is developing in one of the leading industries of this trend. The study contributes by combining and complementing classic and recent theories of partnering and market entry in a partnering framework to provide new insights for de alio market entries, especially on the topics where the academic community solicits contribution. The main research problem focuses on how companies can manage the challenges of de alio market entry by forging partnerships. With whom, why and how to collaborate in the case context? The study uses the qualitative method of single intensive case study, with snowball sampling method, social network analysis and content analysis. The primary empirical data consists of twenty-five semi-structured interviews. The study was conducted in a research project between the case company and the Aalto University School of Business. Big pharmaceutical companies were identified as the most important partnering target (e.g. research collaboration/supplier). To grow the market share, obtain competitive advantage, create and capture value in the biotechnology industry, a heterogeneous portfolio of supporting partnerships is necessary. This includes universities, Key Opinion Leaders in target segments, distribution partners and Contract Research Organizations. The main motivations for partnering are to access (overcome barriers), learn (gain assets), respond to competition, speed up processes and mitigate risks. Adapting the “toolbox” of possible ways how to collaborate in the target industry is important. In biotechnology, a prerequisite for obtaining the partnering benefits is to relax some of the control and compensate it by building long-term win-win relations based on trust, synergy, common goals and aligning business models with the partner.
Towards successful TPACK professional arrangement for in-service teachers

Professional development programmes for technology are essential for teacher capacity to improve students’ achievement. Technological Pedagogical Content Knowledge (TPACK) framework which represents teachers’ knowledge domains, consisting of content, pedagogy, and technology, is used in a number of professional development programmes. This review is aimed at investigating the implementation of in-service teachers’ TPACK professional development arrangements for technology integration using technological pedagogical content knowledge (TPACK) framework. In doing so,this review used five major online databases: ERIC, Web of Science, Scopus, Informaworld, and SpringerLink, with the criteria of studies addressing in-service teachers’ TPACK professional development arrangements for technology integration, except for first aim since it addresses the concept of TPACK; deal with teachers teaching students without disabilities; and must be conducted between 2006 and 2012.In general, the results of this review show three different conceptualisations of TPACK model: initial model of TPACK, ICT-TPACK model, and elaborated TPACK model. Moreover, inquiry learning approach, peer-coaching, authentic learning, problem-based learning, project-based learning, and learning activity typeswere employed in successful TPACK professional development programmes for in-service teachers. In addition, several factors contributing to such successful programmes include engagement, authentic learning experiences, collaboration, supports, curriculum coherency, reflection, feedback, intensive training, and longer time. Finally, teachers were reported having positive experiences with such programmes.