Miracle-dz/UniFAR-FTUs huggingface dataset:
(cid:1) ARK1 Inhibition Improves Survival in a Mouse Model of Heart Failure Induced by Myocardial Infarction

: Heart failure (HF) is characterized by abnormalities in (cid:1) -adrenergic receptor ( (cid:1) AR) signaling, including an increase in (cid:1) AR kinase 1 ( (cid:1) ARK1) levels and activity. Gene therapy using a peptide inhibitor of (cid:1) ARK1 ( (cid:1) ARKct) in infarcted rabbit hearts has improved compromised cardiac function. To determine whether (cid:1) ARK1 inhibition improves survival in a mouse model of HF induced by myocardial infarction (MI), we studied wild-type (WT) and transgenic (TG) mice overexpressing (cid:1) ARKct following MI. There was no difference in infarct size. Survival of WT mice with MI was 25% at 26 weeks. In contrast, 92% of (cid:1) ARKct TG mice with MI survived ( P = 0.01). (cid:1) ARKct TG mice with MI at 8 weeks showed significantly higher fractional shortening compared with WT mice with MI (25.1 ± 2.7% versus 14.2 ± 1.0%; P < 0.05). Moreover, the biochemical (cid:1) AR abnormalities in WT mice with MI were prevented in (cid:1) ARKct TG mice with MI. In conclusion, (cid:1) ARK1 inhibition results in a marked increase in survival and improved cardiac function in a mouse model of HF induced by MI.
India Identified Through On-Farm Observations.

Simple Summary: India has the largest population of dairy cattle in the world, but little work has been done to objectively assess their welfare. Formal welfare assessment is needed to identify any welfare problems and inform solutions to these problems. Dairy cattle on 38 farms in Kerala, India, were observed using standardised welfare assessment protocols. The major welfare problems identiﬁed in this study were that all cows were tied to their housing on a rope < 1 m that attached to a halter that pierced the nasal septum, most farms did not provide cattle with unlimited access to water, and access to food was also limited. On half the farms, cattle were tied up inside for the whole day, and most of those given outdoor access were also tied when outside. These results show the need to encourage dairy farmers in India to stop tying their cattle, and to provide them with unlimited access to drinking water and a readier supply of a good healthy diet. Welfare assessment protocols were used successfully in this study, suggesting that they can and should be used regularly to assess cattle welfare in India. Making these changes could substantially improve the welfare of tens of millions of cattle. Abstract: India has the largest population of dairy cattle in the world at over 48 million animals, yet there has been little formal assessment of their welfare reported. Through observations of dairy cows on 38 farms in Kerala, India, we aimed to investigate the welfare of these animals and the practicality of animal-based assessments within common farming systems. Substantial welfare challenges were identiﬁed. All cows were close-tied (less than 1 m length) via a halter that pierced the nasal septum when housed, which was for the entire day (50% of farms) or part thereof. When outside access was available, it was also usually restricted by close-tying, longline tether, or hobbling. Ad libitum water was only available on 22% of farms and food access was also restricted (mean of 4.3 h / day). Future work should focus on encouraging dairy farmers in India to improve the welfare of their dairy cattle by: ceasing to tie and tether cattle (or at least providing tied and tethered cattle with exercise opportunities); providing unlimited access to drinking water and a readier supply of food (especially quality green forage / fodder); cleaning housing more frequently; providing strategies to prevent heat stress; breeding cattle suited to environmental conditions and with increased resistance to heat stress; and carrying out welfare assessments more regularly using a validated protocol and rectifying the causes of poor welfare. Such changes could substantially improve the welfare of tens of millions of cattle.
High Resolution Mapping of Ribosomal DNA in Early Mouse Embryos by Fluorescence In Situ Hybridization 1

The nucleolar precursor bodies (NPBs) are numerous discrete entities present in the nuclei of early mammalian embryos, which structurally support active rRNA genes. However, whether all rRNA genes, including those not transcribed, are spatially associated with NPBs, and moreover what is the general arrangement of ribosomal DNA (rDNA) in early mouse embryos, still remain unanswered questions. In our study, we examined the localization of rDNA in transcriptionally silent (one-cell and early two-cell) and transcriptionally active (late two-cell) mouse embryos by highly sensitive fluorescence in situ hybridization with probes complementary to mouse rDNA repeats. The results obtained showed that irrespective of the rDNA transcriptional status, one or more NPBs per nucleus were not structurally associated with rDNA. These observations support the idea that NPBs are heterogeneous in their ability to recruit rRNA genes and thus to participate in reassembly of the mature nucleolus. As in somatic cells, and despite the absence of the characteristic nucleoli, the general arrangement of rRNA genes in early mouse embryos reflected the intensity of rDNA transcription. Ribosomal RNA genes were unequally distributed with respect to repeat putative copy numbers between nucleolar organizing region (NOR)-bearing chromosomes at the first cleavage division, and more strikingly, between sister chromatid NORs of a single nucleolar organizing chromosome. The latter indicates that sister chromatids might harbor various numbers of rRNA gene copies, and that the genes might be unequally distributed between the two blastomeres during the first cleavage mitosis.
the presence of schizotypal symptoms aff ects objective and subjective of A of

It is unclear how subclinical symptoms of schizophrenia spectrum pathology impact on Quality of Life in psychiatric patients. It is examined how adult schizotypal symptoms aff ect objective QoL and subjective QoL (OQoL;SQoL) in a clinical cohort. 690 patients of the Department of Child and Adolescent Psychiatry of the University Medical Centre Utrecht, the Netherlands, were reassessed aft er 15.0 years ( SD =5.3) for adult schizotypal symptoms using Schizotypal Personality Questionnaire-Revised. Associations between schizotypal symptoms, distinctive schizotypal symptoms and OQoL were analyzed using (M)ANOVA. The strength of the relation between schizotypal symptoms and seven domains of SQoL were examined by computing Pearson’s bivariate correlations. Partial correlations were computed between each symptom dimension and domain of SQoL while statistically controlling for the other two symptom dimensions. Each schizotypal dimension unfavorably aff ected each domain of OQoL. Negative schizotypal symptoms had the largest eff ect on OQoL. Impoverished SQoL, in particular dissatisfaction with social contacts, was predominantly influenced by negative schizotypal symptoms. The findings show that the extent of schizotypal symptomatology in psychiatric patients already characterized by a decreased level of QoL even further modifies QoL in an unfavorable way. Negative schizotypal symptoms most strongly aff ected OQoL and SQoL. in an way. Negative schizotypal aff ected and SQoL.
Advanced Technologies in Dermatology Advanced Technologies in Dermatology

Cellular therapies are an attractive area of regenerative medicine. For large partial thickness wound, keratinocytes transplant is suggested. The transplantation of cell graft is achieved by obtaining large amounts of cultured cells from a skin biopsy in 3 weeks. Stem cells can be applied before that, but are also efficient in chronic wound closure. Alternative treatment methods are transplants of allogeneic, biostatic skin and amnion. Amnion can be applied as a skin substitute on shallow facialburn wounds, hand burn wounds, on donor areas and granulating wounds. For medium depth or even deep burns, allogeneic skin is recommended. Thanks to the removing of cells from human allogeneic dermis, collagen scaffolding is obtained. It can be populated de novo by autologous skin cells. Artificial skin substitutes are especially good for hand burns and shallow burns. Even though scarring is a part of normal wound healing, it often leads to a pathological process. When scar treatment methods prove insufficient, surgi- cal intervention becomes necessary. Surgical scar intervention involves removal of the pathological skin tissue fragment and replacing it with healthy skin or application of expanders. Improvement of the visual features can be also achieved by laser therapy.
MYELOID NEOPLASIA JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34 + cells in vitro and in vivo

Chronicmyeloidleukemia(CML)stem cell survivalis not dependentonBCR-ABLprotein kinase and treatment with ABL tyrosine kinase inhibitors cures only a minority of CML patients, thus highlighting the need for novel therapeutic targets. The Janus kinase (JAK)2/signal transducer and activator of transcription (STAT)5 pathway has recently beenexploredforprovidingputativesurvivalsignalstoCMLstem/progenitorcells(SPCs) with contradictory results. We investigated the role of this pathway using the JAK2 inhibitor, ruxolitinib (RUX). We demonstrated that the combination of RUX, at clinically achievableconcentrations,withthespecificandpotenttyrosinekinaseinhibitornilotinib, reduced the activity of the JAK2/STAT5 pathway in vitro relative to either single agent alone. These effects correlated with increased apoptosis of CML SPCs in vitro and a reduction inprimitivequiescentCML stemcells, includingNOD.Cg- Prkdc scid IL2rg tm1Wjl /SzJ mice repopulating cells, induced by combination treatment. A degree of toxicity toward normal SPCs was observed with the combination treatment, although this related to mature B-cell engraftment in NOD.Cg- Prkdc scid IL2rg tm1Wjl /SzJ mice with minimal effects on primitive CD34 1 cells. These results support the JAK2/STAT5 pathway as a relevant therapeutic target in CML SPCs and endorse the current use of nilotinib in combination with RUX in clinical trials to eradicate persistent disease in CML patients. ( Blood . 2014;124(9):1492-1501)
Do stars matter? A comparison of operating ratios and indicators among hotels

Hotels use operating ratios and indicators for their performance evaluation. In many countries, including Portugal, there is an official classification of stars for hotels. The rating ranges from 1 star to 5 stars. This rating depends of features of facilities and equipment, reception and concierge service, cleaning and laundry service, food and beverage service and complementary services. Does this classification influence the use of operating ratios and indicators? The Uniform System of Accounts on Lodging Industry (USALI), in its Part III, presents ratios and indicators for all hotel departments. On the other hand, these ratios and indicators could be used to make comparisons among hotels, and there is the possibility of USALI adaptation to all kinds of hotels. This study investigates the relationship between the hotel rating and the use of operating ratios and indicators, as well as their importance. To accomplish the aim and objectives, the Portuguese case was analyzed by conducting a survey to obtain data, which has then been submitted to a statistical analysis. The questionnaire was applied to hotels in Leiria region (87 hotels presented in SIGTUR platform), where was determined a sample of 70 hotels. concluded that, concerning operating indicators and ratios, the most frequently used are: occupancy average daily (ADR), per available (RevPAR). For these ratios there is not any difference of use among hotels, except for RevPAR . Nevertheless, it was found out a relationship between hotel rating and the use of operating ratios and indicators regarding some food and beverage ratios.
Increased pulmonary vascular resistance and defective pulmonary artery ﬁlling in caveolin-1 (cid:1) / (cid:1) mice

Increased pulmonary vascular resistance and defective pulmonary artery ﬁlling in caveolin-1 (cid:1) / (cid:1) mice. Am J Physiol Lung Cell Mol Physiol 294: L865–L873, 2008. First published January 11, 2008; doi:10.1152/ajplung.00079. 2007. —Caveo-lin-1, the structural and signaling protein of caveolae, is an important negative regulator of endothelial nitric oxide synthase (eNOS). We observed that mice lacking caveolin-1 ( Cav1 (cid:1) / (cid:1) ) had twofold increased plasma NO levels but developed pulmonary hypertension. We measured pulmonary vascular resistance (PVR) and assessed alterations in small pulmonary arteries to determine the basis of the hypertension. PVR was 46% greater in Cav1 (cid:1) / (cid:1) mice than wild-type (WT), and increased PVR in Cav1 (cid:1) / (cid:1) mice was attributed to precapillary sites. Treatment with N G -nitro- L -arginine methyl ester ( L -NAME) to inhibit NOS activity raised PVR by 42% in WT but 82% in Cav1 (cid:1) / (cid:1) mice, indicating greater NO-mediated pulmonary vasodilation in Cav1 (cid:1) / (cid:1) mice compared with WT. Pulmonary vasculature of Cav1 (cid:1) / (cid:1) mice was also less reactive to the vasoconstrictor thromboxane A 2 mimetic (U-46619) compared with WT. We ob- served redistribution of type I collagen and expression of smooth muscle (cid:2) -actin in lung parenchyma of Cav1 (cid:1) / (cid:1) mice compared with WT suggestive of vascular remodeling. Fluorescent agarose casting also showed markedly decreased density of pulmonary arteries and artery ﬁlling defects in Cav1 (cid:1) / (cid:1) mice. Scanning electron microscopy showed severely distorted and tortuous pulmonary precapillary vessels. Thus caveolin-1 null mice have elevated PVR that is attributed to remodeling of pulmonary precapillary vessels. The elevated basal plasma NO level in Cav1 (cid:1) / (cid:1) mice compensates partly for the vascular structural abnormalities by promoting pulmonary vasodilation.
Homogeneous analysis of a sample of Open Clusters in the context of the BOCCE project and the Gaia-ESO Survey

The open clusters (OC) are gravitationally bound systems of a few tens or hundreds of stars. In our Galaxy, the Milky Way, we know about 3000 open clusters, of very different ages in the range of a few millions years to about 9 Gyr. OCs are mainly located in the Galactic thin disc, with distances from the Galactic centre in the range 4-22 kpc and a height scale on the disc of about 200 pc. Their chemical properties trace those of the environment in which they formed and the metallicity is in the range -0.5<[Fe/H]<+0.5 dex. Through photometry and spectroscopy it is possible to study relatively easily the properties of the OCs and estimate their age, distance, and chemistry. For these reasons they are considered primary tracers of the chemical properties and chemical evolution of the Galactic disc. The main subject of this thesis is the comprehensive study of several OCs. The research embraces two different projects: the Bologna Open Cluster Chemical Evolution project (BOCCE) and the Gaia-ESO Survey. The first is a long-term programme, aiming at studying the chemical evolution of the Milky Way disc by means of a homogeneous sample of OCs. The latter is a large public spectroscopy survey, conducted with the high-resolution spectrograph FLAMES@VLT and targeting about 10^5 stars in different part of the Galaxy and 10^4 stars in about 100 OCs. The common ground between the two projects is the study of the properties of the OCs as tracers of the disc's characteristics. The impressive scientific outcome of the Gaia-ESO Survey and the unique framework of homogeneity of the BOCCE project can propose, especially once combined together, a much more accurate description of the properties of the OCs. In turn, this will give fundamental constraints for the interpretation of the properties of the Galactic disc.
Early predictors for INSURE failure in preterm infants with neonatal respiratory distress syndrome: a systematic review

The INtubation-SURfactant-Extubation (INSURE) procedure is a widely-used surfactant administration method to treat preterm infants with respiratory distress syndrome (RDS) but is not always successful. We conducted a systematic review to identify early predictive factors for failure of this procedure. A systematic literature search was performed until July 2018 in MEDLINE, EMBASE and the Cochrane Central Register of Controlled Trials (CENTRAL). Original studies comparing INSURE success with INSURE failure in preterm infants with RDS were included. A predefined data extraction form was used to retrieve data from articles and methodological quality was assessed using the SIGN checklists. Fifteen studies out of 690 identified records met inclusion criteria. Methodological quality varied, only 8 studies performed multivariate analysis. We identified 20 different risk factors in total. Evidence for birth weight as a predictor for INSURE failure was inconsistent, but there was a significant association between decreasing gestational age and failure risk. RDS severity was assessed in multiple ways, using arterial blood gas values, imaging and scoring systems. In conclusion: extremely low birth weight, low gestational age and severe RDS appear to be risk factors for INSURE failure. However, evidence is inconsistent due to important methodological heterogeneity. Therefore, clinical applicability of these results is limited and implies the need for future large cohort studies on this subject.
cortisol measurement in the diagnosis of Cushing(cid:144)s

The diagnostic tests required for the biochemical con(cid:147) rmation of Cushing(cid:144)s syndrome (CS) and the underlying cause have been among the most p uz z ling p roblems in clinical endocrinology and remain controversial. D istinguishing betw een CS and situations referred to as p seudo-Cushing states, such as the metabolic syndrome, dep ression, alcoholism can be dif(cid:147) cult, w ith confusing results of biochemical tests. M oreover, w ith increasing aw areness among docters and the w idesp read availability of biochemical testing, p atients are screened in an earlier p hase of their disease, in w hich only minor abnormalities of cortisol secretion are p resent, mak ing biochemical con(cid:147) rmation of hyp ercortisolism even more dif(cid:147) ccult. In the case of clinical susp icion of CS, biochemical screening for hyp ercortisolism can be p erformed. Tw enty-four hour urine collection for the measurement of urinary free cortisol and the low -dose dex amethasone sup p ression test (1 mg) have been used ex tensively as (cid:147) rst line screenings test for CS, but neither test has p roven fully cap able of distinguishing all cases of CS from other individuals. Since several years, measuring late night salivary cortisol concentration has emerged as another screening test for the diagnosis of Cushing(cid:144)s syndrome. In the p resent rep ort, several asp ects of salivary cortisol measurement in the diagnosis of Cushing(cid:144)s syndrome are discussed. R elationship betw een p lasma and salivary cortisol concentration, collection methods, technical asp ects of measuring salivary cortisol concentration, esp ecially the validation of an automated assay on the R oche immunoanalyz er, confounders and establishment of reference ranges are described. F urthermore, p ublished data on the clinical use of late-night salivary cortisol
Assessment of Factors Controlling Clumped Isotopes and d 18 O Values of Hydrothermal Vent Calcites Geochemistry, Geophysics, Geosystems

The clumped isotope composition of CaCO 3 ( D 47 ) is a geochemical proxy that can provide mineral formation temperatures and, together with measured carbonate d 18 O, inferred ﬂuid d 18 O values. Under natural conditions, carbonates form within a relatively wide pH range and varying growth rates which are typically not reﬂected in laboratory-based calibrations (mostly (cid:2) pH 8, moderate growth rates). A pH and growth-rate dependence is known for oxygen isotopes and was also postulated for clumped isotopes. Theoretical predictions suggest that D 47 values could lie between the carbonate equilibrium value and the value inherited from the dissolved inorganic carbon (predicted offset: 1 0.04 & pH < 4 and 2 0.025 & at high pH > 12). Here we test whether pH (in addition to temperature) is recorded in the carbonate clumped isotope composition using modern calcites from natural travertine-forming streams and scales precipitated in pipes of deep geothermal wells from Italy, Hungary, and Turkey (pH: 6.1–7.5, T: 33–100 8 C). Although a comparison of all samples with expected equilibrium values in this pH range and known formation temperatures reveals only an insigniﬁcant D 47 offset (0.006 6 0.004 & , 1SE, n 5 9), the clumped isotope values of samples with the highest growth rates (0.014 6 0.007 & , 1SE, n 5 5) are consistent with the theoretical prediction attributable to pH of 0.01 & . Similarly, deviations in d 18 O of up to 2 2 & follow a growth-rate dependence. This ﬁeld-based study shows that pH-related effects are mostly small for D 47 in the subsurface environment at lower pH and that high mineral growth rates control the magnitude of this disequilibrium.
The Bartle-Dunford-Schwartz integral : Integration with respect to Sigma-Additive vector measure

Preliminaries. - Basic Properties of the Bartle-Dunford-Schwartz Integral. - Lp-spaces, 1 ? p ? - Integration With Respect to lcHs-valued Measures. - Applications to Integration in Locally Compact Hausdorff Spaces - Part I. - Applications to Integration in Locally Compact Hausdorff Spaces - Part II. - Complements to the Thomas Theory of Vectorial Radon Integration.
In ﬂ uence of Wind Direction on Thermodynamic Properties and Arctic Mixed-Phase Clouds in Autumn at Utqia ġ vik, Alaska Geophysical

Seven years of autumnal ground-based observations (September – November 2002 – 2008) at the U.S. Energy Atmospheric Radiation Measurement North Slope of Alaska site have been analyzed for addressing the occurrence frequency and macrophysical and microphysical properties of Arctic mixed-phase clouds (AMC), as well as the relationship between environmental parameters and AMC properties. In September and October, AMC occurrence frequency is 20 – 30% lower during a southerly wind when compared to the other wind directions; in November, the variation of AMC occurrence frequency with wind direction is small. The mean liquid water path in November is about half of that in October and September. When the surface is snow free, temperature ( T ) and speci ﬁ c humidity ( q ) pro ﬁ les during a northerly wind are warmer and moister than those for the southerly wind. Northerly wind pro ﬁ les have a higher relative humidity to ice (RH i ) and lower atmosphere stability. Furthermore, the AMC occurrence frequency has a positive relationship with RH i and a negative relationship with stability. These two points may explain the lower AMC occurrence frequency during a southerly wind. During a northerly wind, AMCs have larger radar re ﬂ ectivity, wider spectrum width, and larger Doppler velocity signatures. The stronger precipitation for AMC during a northerly wind is possibly due to the cleaner air masses from the ocean (north). With the same amount of q , the radar spectrum width has a higher frequency in the larger bins during a northerly wind. Both T , q , and radar re ﬂ ectivity, radar spectrum width pro ﬁ les show evidence of deposition in the sub-cloud layer in September and October. Mixed-phase cloud (AMC), the cloud heights of AMC, the liquid water path (LWP) of AMC, the atmospheric temperature/speci ﬁ c humidity/RH i /stability pro ﬁ les when AMCs are present, and the radar re ﬂ ectivity/ spectrum width/Doppler velocity of AMCs. In addition, we investigate whether the above parameters vary with different wind direction (northerly versus southerly) and surface condition (snow free versus snow/ice). From these analyses, the following conclusions can be made: