Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of protein kinases during postnatal development of rat heart

The loss of ability to proliferate, the shift in the energy dependency from carbohydrate to fatty acids and the reduction in capability to resist ischemia are some of the key phenomena observed during postnatal development of the heart. These distinct phenotypic changes can be attributed to the regulation of intracellular signal transduction proteins. To investigate how these protein kinases are expressed during postnatal development, 44 different protein kinases were studied by Western blotting analysis. Among them protein kinases, implicated in cell proliferation and cell cycle including protein kinase C, cyclin-dependent protein kinases and mitogenic M A P kinases, declined during the postnatal development. The specific activities of E r k l and Erk2 declined with age, which was concurrent to the decrease of C D K 1 activity. The protein kinases involved in stress-responses such as M l k 3 , M e k k l , S e k l , M k k 3 and Mapkapk2, increased in expression during postnatal development. The proto-oncogene-encoded kinases Mos and Tpl2 were up-regulated. The expression of the insulin-activated kinases (PI3K, P K B , S 6 K and C K 2 ) was downregulated by 40-60% with age. The activities of P K B , S 6 K and C K 2 were also decreased with age. Tissue distribution studies of adult rats revealed that most of the protein kinases that were up-regulated during heart development tended to be preferentially expressed in the heart, whereas the down-regulated protein kinases were generally expressed in the heart at relatively lower amounts than in most other tissues. To investigate the signalling pathways of these kinases, isolated cardiomyocytes or isolated whole hearts were treated with various agonists. These results indicate that E r k l can be regulated via P K C but not by c A P K nor c G P K , whereas Mapkapk2 can be controlled by c G P K
Transient Receptor Potential Ankyrin-1 and Vanilloid-3 Differentially Regulate Endoplasmic Reticulum Stress and Cytotoxicity in Human Lung Epithelial Cells After Pneumotoxic Wood Smoke Particle Exposure

This study investigated the roles of transient receptor potential (TRP) ankyrin-1 (TRPA1) and TRP vanilloid-3 (TRPV3) in regulating endoplasmic reticulum stress (ERS) and cytotoxicity in human bronchial epithelial cells (HBECs) treated with pneumotoxic wood smoke particulate matter (WSPM) and chemical agonists of each channel. Functions of TRPA1 and TRPV3 in pulmonary epithelial cells remain largely undefined. This study shows that TRPA1 activity localizes to the plasma membrane and endoplasmic reticulum (ER) of cells, whereas TRPV3 resides primarily in the ER. Additionally, treatment of cells using moderately cytotoxic concentrations of pine WSPM, carvacrol, and other TRPA1 agonists caused ERS as a function of both TRPA1 and TRPV3 activities. Specifically, ERS and cytotoxicity were attenuated by TRPA1 inhibition, whereas inhibiting TRPV3 exacerbated ERS and cytotoxicity. Interestingly, after treatment with pine WSPM, TRPA1 transcription was suppressed, whereas TRPV3 was increased. TRPV3 overexpression in HBECs conferred resistance to ERS and an attenuation of ERS-associated cell cycle arrest caused by WSPM and multiple prototypical ERS-inducing agents. Alternatively, short hairpin RNA–mediated knockdown of TRPV3, like the TRPV3 antagonist, exacerbated ERS. This study reveals previously undocumented roles for TRPA1 in promoting pathologic ERS and cytotoxicity elicited by pneumotoxic WSPM and TRPA1 agonists, and a unique role for TRPV3 in fettering pathologic facets of the integrated ERS response. SIGNIFICANCE STATEMENT These findings provide new insights into how wood smoke particulate matter and other transient receptor potential ankyrin-1 (TRPA1) and transient receptor potential vanilloid-3 (TRPV3) agonists can affect human bronchial epithelial cells and highlight novel physiological and pathophysiological roles for TRPA1 and TRPV3 in these cells.
Characterization of Tissue-Engineered Human Periosteum and Allograft Bone Constructs: The Potential of Periosteum in Bone Regenerative Medicine

Delayed-union or non-union between a host bone and a graft is problematic in clinical treatment of segmental bone defects in orthopedic cases. Based on a preliminary study of human periosteum allografts from this laboratory, the present work has extensively investigated the use of human cadaveric tissue-engineered periosteum-allograft constructs as an approach to healing such serious orthopedic surgical situations. In this current report, human cadaveric periosteum-wrapped bone allografts and counterpart controls without periosteum were implanted subcutaneously in athymic mice (nu/nu) for 10, 20, and, for the first time, 40 weeks. Specimens were then harvested and assessed by histological and gene expression analyses. Compared to controls, the presence of new bone formation and resorption in periosteum-allograft constructs was indicated in both histology and gene expression results over 40 weeks of implantation. Of several genes also examined for the first time, RANKL and SOST expression levels increased in a statistically significant manner, data suggesting that bone formation and the presence of increasing numbers of osteocytes in bone matrices had increased with time. The tissue-engineering strategy described in this study provides a possible means of improving delayed-union or non-union at the healing sites of segmental bone defects or bone fractures. The potential of periosteum and its resident cells could thereby be utilized effectively in tissue-engineering methods and tissue regenerative medicine.
The relationship between posttraumatic stress disorder, trauma centrality, interpersonal sensitivity and psychiatric co-morbidity among students in Kazakhstan: a Latent Class Analysis

Abstract Background Trauma can lead to trauma centrality and affect levels of interpersonal sensitivity and psychiatric co-morbidity. Whether a coexisting relationship between posttraumatic stress disorder (PTSD) and trauma centrality can influence levels of interpersonal sensitivity and psychiatric co-morbidity among university students from Kazakhstan is unknown. Aim To investigate the impact of the aforementioned co-existing relationship on interpersonal sensitivity and psychiatric co-morbidity among Kazakh university students. Methods 597 students (F = 428, M = 169) completed questionnaires measuring PTSD, psychiatric co-morbidity, interpersonal sensitivity, and trauma centrality. Results 28%, 32% and 40% met the criteria for full, partial and no-PTSD, respectively. Latent Class Analysis revealed a three-class solution: Class 1 (the altered-self group) with a low level of PTSD but a high level of trauma centrality, Class 2 (the traumatized-self group) with high levels of PTSD and trauma centrality and Class 3 (the low symptom group) with low levels of PTSD and trauma centrality. There were significant differences in the levels of interpersonal sensitivity and psychiatric co-morbidity across three classes. Conclusion There are individual differences in the display of posttraumatic stress disorder symptoms, and trauma centrality. These differences can influence interaction with others and psychological distress.
Copernicia Prunifera Leaf Fiber: A Promising New Reinforcement for Epoxy Composites

A basic characterization of novel epoxy matrix composites incorporated with up to 40 vol% of processed leaf fibers from the Copernicia prunifera palm tree, known as carnauba fibers, was performed. The tensile properties for the composite reinforced with 40 vol% of carnauba fibers showed an increase (40%) in the tensile strength and (69%) for the elastic modulus. All composites presented superior elongation values in comparison to neat epoxy. Izod impact tests complemented by fibers/matrix interfacial strength evaluation by pullout test and Fourier transformed infrared (FTIR) analysis revealed for the first time a significant reinforcement effect (> 9 times) caused by the carnauba fiber to polymer matrix. Additional thermogravimetric analysis (TG/DTG) showed the onset of thermal degradation for the composites (326 ~ 306 °C), which represents a better thermal stability than the plain carnauba fiber (267 °C) but slightly lower than that of the neat epoxy (342 °C). Differential scanning calorimetry (DSC) disclosed an endothermic peak at 63 °C for the neat epoxy associated with the glass transition temperature (Tg). DSC endothermic peaks for the composites, between 73 to 103 °C, and for the plain carnauba fibers, 107 °C, are attributed to moisture release. Dynamic mechanical analysis confirms Tg of 64 °C for the neat epoxy and slightly higher composite values (82–84 °C) due to the carnauba fiber interference with the epoxy macromolecular chain mobility. Both by its higher impact resistance and thermal behavior, the novel carnauba fibers epoxy composites might be considered a viable substitute for commonly used glass fiber composites.
Rapid Diagnostic Tests for Trypanosoma cruzi Infection: Field Evaluation of Two Registered Kits in a Region of Endemicity and a Region of Nonendemicity in Argentina

Infection by Trypanosoma cruzi (Chagas disease [ChD]) affects around 7 million people in the Americas, most of whom are unaware of their status due to lack of clinical manifestations and poor access to diagnosis. Rapid diagnostic tests (RDTs) are widely used for screening for different infections (HIV, hepatitis B, and syphilis), and their application for ChD would facilitate access to diagnosis, especially in remote areas where health services have scarce resources. We conducted a prospective intervention study in 2018 to evaluate in the field two in vitro RDTs for ChD, authorized by the National Administration of Medicaments, Aliments, and Medical Technologies of Argentina (ANMAT), in areas of endemicity and nonendemicity in Argentina. ABSTRACT Infection by Trypanosoma cruzi (Chagas disease [ChD]) affects around 7 million people in the Americas, most of whom are unaware of their status due to lack of clinical manifestations and poor access to diagnosis. Rapid diagnostic tests (RDTs) are widely used for screening for different infections (HIV, hepatitis B, and syphilis), and their application for ChD would facilitate access to diagnosis, especially in remote areas where health services have scarce resources. We conducted a prospective intervention study in 2018 to evaluate in the field two in vitro RDTs for ChD, authorized by the National Administration of Medicaments, Aliments, and Medical Technologies of Argentina (ANMAT), in areas of endemicity and nonendemicity in Argentina. We recruited 607 volunteers older than 18 years in Salta province and the city of Buenos Aires. The RDTs Ab Standard Diagnostics SD Bioline (SD) and Check Chagas Wiener Lab (WL) were performed in situ with whole-blood samples, and confirmatory serology was done at a reference center. The rate of infection with T. cruzi was 17.8% (108/607). The SD test showed 97.2% sensitivity (95% confidence interval [CI], 93.5 to 100) and 91.7% specificity (95% CI, 96.2 to 99.2%), and the WL test showed 93.4% sensitivity (95% CI, 88.2 to 98.6%) and 99.1% specificity (95% CI, 91.9 to 100%). The sensitivity and specificity for the two RDTs tested were higher than previously reported. These results encourage the use of the tested RDTs in Salta province and for further field studies for the implementation of these RDTs in other epidemiological scenarios. This will be very important to improve access to diagnosis of Chagas and its clinical management as a neglected disease, especially in remote areas with health access barriers.
Metabolomics Fingerprint Induced by the Intranigral Inoculation of Exogenous Human Alpha-Synuclein Oligomers in a Rat Model of Parkinson’s Disease

Parkinson’s disease (PD) is considered a synucleinopathy because of the intraneuronal accumulation of aggregated α-synuclein (αSyn). Recent evidence points to soluble αSyn-oligomers (αSynO) as the main cytotoxic species responsible for cell death. Given the pivotal role of αSyn in PD, αSyn-based models are crucial for the investigation of toxic mechanisms and the identification of new therapeutic targets in PD. By using a metabolomics approach, we evaluated the metabolic profile of brain and serum samples of rats infused unilaterally with preformed human αSynOs (HαSynOs), or vehicle, into the substantia nigra pars compacta (SNpc). Three months postinfusion, the striatum was dissected for striatal dopamine (DA) measurements via High Pressure Liquid Chromatography (HPLC) analysis and mesencephalon and serum samples were collected for the evaluation of metabolite content via gas chromatography mass spectrometry analysis. Multivariate, univariate and correlation statistics were applied. A 40% decrease of DA content was measured in the HαSynO-infused striatum as compared to the contralateral and the vehicle-infused striata. Decreased levels of dehydroascorbic acid, myo-inositol, and glycine, and increased levels of threonine, were found in the mesencephalon, while increased contents of fructose and mannose, and a decrease in glycine and urea, were found in the serum of HαSynO-infused rats. The significant correlation between DA and metabolite content indicated that metabolic variations reflected the nigrostriatal degeneration. Collectively, the metabolomic fingerprint of HαSynO-infused rats points to an increase of oxidative stress markers, in line with PD neuropathology, and provides hints for potential biomarkers of PD.
Water-ion permselectivity of narrow-diameter carbon nanotubes

Small diameter carbon nanotubes show high water-salt permselectivity and low activation barrier for water transport. Carbon nanotube (CNT) pores, which mimic the structure of the aquaporin channels, support extremely high water transport rates that make them strong candidates for building artificial water channels and high-performance membranes. Here, we measure water and ion permeation through 0.8-nm-diameter CNT porins (CNTPs)—short CNT segments embedded in lipid membranes—under optimized experimental conditions. Measured activation energy of water transport through the CNTPs agrees with the barrier values typical for single-file water transport. Well-tempered metadynamics simulations of water transport in CNTPs also report similar activation energy values and provide molecular-scale details of the mechanism for water entry into these channels. CNTPs strongly reject chloride ions and show water-salt permselectivity values comparable to those of commercial desalination membranes.
Fixed-Time Prescribed Tracking Control for Stochastic Nonlinear Systems With Unknown Measurement Sensitivity

This article is concerned with the fixed-time prescribed tracking control problem for the uncertain stochastic nonlinear systems subject to input quantization and unknown measurement sensitivity. Different from existing results, the sensitivity on the sensor for measuring the system state is considered as an unknown parameter instead of the known one. Due to unknown measurement sensitivity on the sensor, the real system state cannot be obtained by measurement; hence, we put forward a new feedback control algorithm by the use of the unreal measured value of the system state. Moreover, the fixed-time prescribed performance on the output tracking error is investigated by developing a novel performance function. By means of the backstepping method, an adaptive quantized controller is designed for the system. Based on the Lyapunov stability theory, it is proved that the controller can render the output tracking error that satisfies the fixed-time prescribed performance and all signals of the resulting closed-loop system are bounded in probability. Finally, simulation results are provided to illustrate the effectiveness of the proposed control algorithm.
Navigating the social world: Toward an integrated framework for evaluating self, individuals, and groups.

Social evaluation occurs at personal, interpersonal, group, and intergroup levels, with competing theories and evidence. Five models engage in adversarial collaboration, to identify common conceptual ground, ongoing controversies, and continuing agendas: Dual Perspective Model (Abele & Wojciszke, 2007); Behavioral Regulation Model (Leach, Ellemers, & Barreto, 2007); Dimensional Compensation Model (Yzerbyt et al., 2005); Stereotype Content Model (Fiske, Cuddy, Glick, & Xu, 2002); and Agency-Beliefs-Communion Model (Koch, Imhoff, Dotsch, Unkelbach, & Alves, 2016). Each has distinctive focus, theoretical roots, premises, and evidence. Controversies dispute dimensions: number, organization, definition, and labeling; their relative priority; and their relationship. Our first integration suggests 2 fundamental dimensions: Vertical (agency, competence, "getting ahead") and Horizontal (communion, warmth, "getting along"), with respective facets of ability and assertiveness (Vertical) and friendliness and morality (Horizontal). Depending on context, a third dimension is conservative versus progressive Beliefs. Second, different criteria for priority favor different dimensions: processing speed and subjective weight (Horizontal); pragmatic diagnosticity (Vertical); moderators include number and type of target, target-perceiver relationship, context. Finally, the relation between dimensions has similar operational moderators. As an integrative framework, the dimensions' dynamics also depend on perceiver goals (comprehension, efficiency, harmony, compatibility), each balancing top-down and bottom-up processes, for epistemic or hedonic functions. One emerging insight is that the nature and number of targets each of these models typically examines alters perceivers' evaluative goal and how bottom-up information or top-down inferences interact. This framework benefits theoretical parsimony and new research. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Production of donor-derived eggs after ovarian germ cell transplantation into the gonads of adult, germ cell-less, triploid hybrid fish †

Abstract In animals, spermatogonial transplantation in sterile adult males is widely developed; however, despite its utility, ovarian germ cell transplantation is not well developed. We previously showed that the interspecific hybrid offspring of sciaenid was a suitable model for germ cell transplantation studies as they have germ cell-less gonads. However, all these gonads have testis-like characteristics. Here, we tested whether triploidization in hybrid embryos could result in germ cell-less ovary development. Gonadal structure dimorphism and sex-specific gene expression patterns were examined in 6-month-old triploid hybrids (3nHybs). Thirty-one percent of 3nHybs had germ cell-less gonads with an ovarian cavity. cyp19a1a and foxl2, ovarian differentiation-related genes, were expressed in these gonads, whereas dmrt1 and vasa were not expressed, suggesting ovary-like germ cell-less gonad development. Some (26%) 3nHybs had testis-like germ cell-less gonads. Ovarian germ cells collected from homozygous green fluorescent protein (GFP) transgenic blue drum (BD) (Nibea mitsukurii) were transplanted into 6-month-old 3nHybs gonads via the urogenital papilla or oviduct. After 9 months, the recipients were crossed with wild type BD. Among the six 3nHyb recipients that survived, one female and one male produced fertile eggs and motile sperm carrying gfp-specific DNA sequences. Progeny tests revealed that all F1 offspring possessed gfp-specific DNA sequences, suggesting that these recipients produced only donor-derived eggs or sperm. Histological observation confirmed donor-derived gametogenesis in the 3nHyb recipients' gonads. Overall, triploidization reduces male-biased sex differentiation in germ cell-less gonads. We report, for the first time, donor-derived egg production in an animal via direct ovarian germ cell transplantation into a germ cell-less ovary. Summary sentence Using triploid hybrid fish with ovary-like germ cell-less gonads, production of donor-derived gametes following ovarian germ cell transplantation into sterile adult gonads was achieved for the first time in an animal.
Synthesis and Characterization of Novel Resveratrol Butyrate Esters That Have the Ability to Prevent Fat Accumulation in a Liver Cell Culture Model

To facilitate broad applications and enhance bioactivity, resveratrol was esterified to resveratrol butyrate esters (RBE). Esterification with butyric acid was conducted by the Steglich esterification method at room temperature with N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC) and 4-dimethyl aminopyridine (DMAP). Our experiments demonstrated the synthesis of RBE through EDC- and DMAP-facilitated esterification was successful and that the FTIR spectra of RBE revealed absorption (1751 cm−1) in the ester region. 13C-NMR spectrum of RBE showed a peak at 171 ppm corresponding to the ester group and peaks between 1700 and 1600 cm−1 in the FTIR spectra. RBE treatment (25 or 50 μM) decreased oleic acid-induced lipid accumulation in HepG2 cells. This effect was stronger than that of resveratrol and mediated through the downregulation of p-ACC and SREBP-2 expression. This is the first study demonstrating RBE could be synthesized by the Steglich method and that resulting RBE could inhibit lipid accumulation in HepG2 cells. These results suggest that RBE could potentially serve as functional food ingredients and supplements for health promotion.
Teacher Turnover and Access to Effective Teachers in the School District of Philadelphia

Concerned about the expense of teacher turnover, its disruption to schools and students, and its potential effect on students’ access to effective teachers, the School District of Philadelphia (SDP) partnered with the Regional Educational Laboratory Mid-Atlantic to better understand students’ access to effective teachers and the factors related to teacher turnover. The district already engages in efforts to address teacher turnover by supporting new teachers, targeting retention strategies to teacher needs, and improving school climate. Findings from this study can inform these efforts by improving SDP’s understanding of the role of turnover in the equitable distribution of effective teachers and by identifying teacher and school characteristics related to turnover.
EVALUATION OF NEWCASTLE DISEASE VIRUS MATERNALLY DERIVED ANTIBODIES IN QUAIL CHICKS FOR ESTIMATION OF PROPER VACCINATION TIME

The present study was conducted to monitor the persistence of Maternal Derived Antibody (MDA) in quail chicks during first five weeks of their life and its effect on the immune response to Newcastle disease virus vaccination (LaSota and Hitchner B1). The obtained results showed that, the MDA HI titers ranged from 5 to 7 (log2) with a geometric mean of 6.2 6.4 (log2) at the end of first week. At the end of the second week of age (day 14) the MDA HI titers were decreased with a mean titer of 5.1 5.2 (log2). At the end of the third week, only 40% of quail chicks (4/10) showed titers more than 5 (log2), whereas 60% of the quail chicks titers less than 4(log2) in HI test. One week later (day 28), all of the quail chicks showed titers less than 4 (log2) in HI test. At the end of the fifth week, (day 35), the mean titer was decreased to its minimum level equal to (≤ 2.0 log2). The obtained results revealed that, the mean value of HI antibody titers declined in the first two weeks post vaccination with (LaSota and Hitchner B1) when vaccination take place at 7 day old, whereas higher and persistence antibody response takes place when vaccination applied at 14 or 21 day old.