Miracle-dz/UniFAR-FTUs huggingface dataset:
OTT_A_251926 6143..6156

Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA Purpose: High-risk human papillomaviruses (HR HPV) cause cervical cancer, and in these cancers, HPV type 16 is the most common HR type. The HR viral oncogenes E6 and E7 partner with cellular proteins to drive cancer and modulate immune pathways; previously, we demonstrated in keratinocytes that HPV 16 E6 and high expression of the endogenous host protein partner NFX1-123 led to the increased expression of multiple genes, including Notch1, secretory leukocyte peptidase inhibitor (SLPI), and retinoic acid early transcript 1G (RAET1G). The present study was conducted to determine if NFX1-123 was highly expressed in cervical cancer and if genes increased by NFX1-123 and 16E6 in keratinocytes were also increased in cervical cancers. Materials and Methods: The Cancer Genome Atlas (TCGA) database and The Human Protein Atlas database were used to compare relative mRNA and protein gene expression, respectively, in the normal cervix and cervical cancers. Formalin-fixed paraffin-embedded (FFPE) normal cervix and HPV 16 positive cervical cancer samples were analyzed for relative protein expression by immunohistochemical staining. Protein expression of a subset of regulated genes was quantified by Western blot of HPV positive and negative cell lines. Results: Immunohistochemical staining of HPV 16 positive cervical dysplasias and cancers revealed high NFX1-123, Ki67, and Notch1 expression. NFX1 and NFX1L1 mRNA levels were increased in cervical cancers compared to normal cervix in the TCGA database. Fourteen genes previously identified as upregulated in keratinocytes with 16E6 and overexpressed NFX1-123 also had high mRNA expression and selected genes had high protein expression in cervical cancers and cell lines. Conclusion: In cervical cancer, NFX1-123 is highly expressed, and 16E6 and NFX1-123 together alter the expression of a wide set of genes. The involvement of these genes in cell proliferation, differentiation, invasion, and metastasis provides further insight into potential ways that HR HPVs promote cancer initiation and maintenance.
Potential Use of Lactobacillus plantarum 1UHCC as a Bio-hydrolyzer in the Development of the Sustainable Food Industry in Indonesia

The purpose of this study aimed to evaluate the potential and performance of Lactobacillus plantarum 1 UHCC (L.plantarum 1UHCC) bacteria as bio-hydrolyzer of the food industry in Indonesia. As much as 100g of fresh hide from Balinese cattle were used as a substrate. The fermentation time was carried out at 3 time levels, namely: 24 hours (T-24); 48 hours (T-48) and 72 hours (T-72). The bacteria were used L.plantarum which was isolated from milkfish extract. The results showed that in the solution of L.plantarum bacteria using collagen substrate of cattle hide, pH values were increased and the lactic acid levels were decreased significantly. The pH value and the lactic acid level of the solution were respectively around 5.77±0.03-6.13±0.02 and 1.65±0.26-2.02±0.05%. The total bacteria has increased and dissolved protein has decreased for fermentation time, but in general it is not significant. Total bacteria and dissolved proteins respectively 4.5±0.98-5.5±2.29 Log10CFU/mL and 36.98±3.37-40.25±1.54 mg/mL. The application of 24 hours (T-24) fermentation time to L.plantarum bacteria using collagen substrate of cattle hide skin was considered as the optimum time to be applied in the fermentation process. Bacteria L.plantarum 1UHCC has the ability to hydrolyze collagen protein components, especially collagen extracts from cattle hide. Bacteria L.plantarum 1UHCC has the potential to be developed as a bio-hydrolyzer organism. Bacteria L.plantarum 1UHCC plays a very important role in the development of the food industry, especially in Indonesia. Keywords: Bacteria, Cattle hide, Collagen, Fermentation, L.plantarum 1UHCC
Pork skin connective tissue gel utilization in reduced-fat bologna

This study determined temperature (50 degrees, 60 degrees, 70 degrees and 80 degrees C) and time (0.5, 1.0, 1.5 and 2.0h) factors that enhanced the water binding of pork skin connective tissue (PCT); the functionality of added water (AW, 100-600%, w/w) PCT gels; and attributes of bologna containing 10-30% addition of 100-600% AW PCT gels. Heating (70 degrees C) PCT increased water binding. Gels (100-600% AW) were formed by heating PCT (70 degrees C) for 30 min. Higher AW levels increased (P<0.05) gel moisture content, while decreasing fat, melting points, collagen content, and hardness. Addition of PCT gels in bologna decreased (P<0.05) hardness and increased juiciness, indicating the potential of PCT gels as water binders and texture-modifying agents.
Ionizing radiation induces apoptotic cell death in human TcR‐γ/δ+ T and natural killer cells without detectable p53 protein

The p53 tumor suppressor gene has been shown to be involved in programmed cell death, apoptosis, in murine immature thymocytes after treatment with ionizing radiation. Ionizing radiation also induces apoptosis in peripheral mature lymphocytes. In this work, we investigated the p53 participation in radiation‐induced apoptosis in human peripheral blood lymphocytes (PBL) subpopulations. Exposure to γ‐irradiation resulted in an appreciable induction of apoptotic cell death in TcR‐α/β+ (CD4+ and CD8+) T cells, TcR‐γ/δ+ T cells, B cells and natural killer (NK) cells, as assessed by DNA fragmentation as well as the morphological characteristics. Importantly, it was found that there was a marked difference among PBL subpopulations as regards the induction of p53 protein by γ‐irradiation. Similar to previous observations for murine thymocytes, p53 induction in TcR‐α/β+ T cells and B cells after γ‐irradiation was evident by Western blot analysis. Radiation‐induced apoptosis in TcR‐α/β+ T cells and B cells was efficiently inhibited by cycloheximide, indicating the requirement of de novo protein synthesis, including p53 protein, for radiation‐induced apoptosis in both subpopulations. In marked contrast, no identifiable levels of p53 protein were induced in either TcR‐γ/δ+ T or NK cells after γ‐irradiation. In addition, it was demonstrated that radiation‐induced cell death in TcR‐γ/δ+ T and NK cells could be prevented by interleukin‐2, but not by cycloheximide. These results imply that radiation‐induced lymphocytic apoptosis can be mediated by p53‐dependent or ‐independent mechanisms.
Quantifying primary production of microphytobenthos: application of [novel] optical methods

We investigated the hyper-spectral reflectance of intertidal sediments during the summer in a number of European estuaries with different sediment characteristics. At each site, grids or transects were established. At each grid node, a single sample for grain size and organic content analysis was collected as well as 3 paired replicate measurements of hyper-spectral reflectance, minimum fluorescence after 15 min dark adaptation (FBoPB 15 P), sediment water content (abs.) (% weight) and surface sediment (approx. 2mm) chlorophyll a + breakdown product concentrations ([chl a + phaeo] mg chl a m PP). The spectral signatures of tidal flats dominated by benthic microalgae, mainly diatoms, could be easily distinguished from sites dominated by macrophytes. The normalized difference vegetation index (NDVI) was found to be most strongly correlated to sediment [chl a + phaeo], although examination of correlations within each grid revealed that NDVI and sediment [chl a + phaeo] was not significantly correlated within the predominantly sandy Sylt grids. F BoPB 15 P was also significantly
An Extensible Multi-Sensor Fusion Framework for 3D Imaging

Many autonomous vehicles rely on an array of sensors for safe navigation, where each sensor captures different visual attributes from the surrounding environment. For example, a single conventional camera captures high-resolution images but no 3D information; a LiDAR provides excellent range information but poor spatial resolution; and a prototype single-photon LiDAR (SP-LiDAR) can provide a dense but noisy representation of the 3D scene. Although the outputs of these sensors vary dramatically (e.g., 2D images, point clouds, 3D volumes), they all derive from the same 3D scene. We propose an extensible sensor fusion framework that (1) lifts the sensor output to volumetric representations of the 3D scene, (2) fuses these volumes together, and (3) processes the resulting volume with a deep neural network to generate a depth (or disparity) map. Although our framework can potentially extend to many types of sensors, we focus on fusing combinations of three imaging systems: monocular/stereo cameras, regular LiDARs, and SP-LiDARs. To train our neural network, we generate a synthetic dataset through CARLA that contains the individual measurements. We also conduct various fusion ablation experiments and evaluate the results of different sensor combinations.
Effects of Forearm Myofascial Trigger Point Dry Needling on Pain and Function of Patients With Carpal Tunnel Syndrome

Introduction Carpal tunnel syndrome (CTS) is the most common entrapment neuropathy. The common symptoms of CTS include numbness, paresthesia, weakness, and pain in the areas of the hand innervated by the median nerve. Conservative treatments can relieve complains of mild and moderate CTS, including medication, splinting, local injection, and manual therapies (1-3). In addition, myofascial pain syndrome (MFPS) with trigger points in the muscle is a common musculoskeletal problem. Trigger points can cause motor, sensory, and even autonomic dysfunction, as well as referred pain (46). Dry needling (DN) uses very thin hollow-core needles that are subcutaneously inserted into trigger points. Several studies have investigated the effectiveness of this method on myofascial pain in different parts of the body (7-9). There is no clear mechanism regarding the association between CTS and forearm myofascial pain. Few studies have reported the possible correlation of the occurrence of CTS in patients with MFPS (10,11). Forearm muscles including brachioradialis and extensor carpi radialis get overworked by excessive gripping motions thus the trigger points in these muscles are relatively common which can produce pain at the elbow and referred pain in the wrist, the base of the thumb, and the dorsum of the hand, and finally, exacerbate CTS symptoms (5). Considering the high prevalence of CTS and MFPS, these two conditions may occur simultaneously and exacerbate clinical symptoms. The trigger point DN may offer a promising therapeutic option to alleviate symptoms in these patients although there is a lack of studies validating this treatment in CTS. Accordingly, the aim of the present study was to determine the frequency of the co-occurrence of CTS with forearm myofascial trigger points (MTrPs) and the effects of one-session DN on symptoms in these patients. It was hypothesized that forearm MTrP DN in combination with other treatments would exhibit more CTS symptom reductions.
Prevalence of caries in mandibular first molar and treatment needs in 15 -17 year old students in Calicut district, Kerala, India

Background: Permanent first molars occupy a strategic position in the dental arch, so it is necessary to know the caries prevalence in this tooth so that adequate measures can be taken to prevent caries progression or development of new carious lesions. No studies have been conducted till date to evaluate the prevalence of caries in first molars in Calicut district of Kerala, India. Hence, the aim of this present study was to evaluate the prevalence of caries in first molars in Calicut district in 15-17 year old students. Materials and methods: This school based cross-sectional study examined a total of 3200 students after obtaining the institutional ethical clearance and permission from the school authorities. Students were examined and data was collected and recorded based on a questionnaire to evaluate the association of caries in mandibular first molar with respect to age, gender, dietary habits and location. Written consent was obtained from each student prior to the examination. All the data were entered in SPSS (18) software. Both descriptive and analytic approaches were used in data analysis. Mean and standard deviation was calculated for quantitative variables and frequency for qualitative variables. The prevalence was expressed in percentage. Chi-square test was used to compare socio-demographic characteristics and risk factors (oral hygiene practises and personal habits). The level of statistical significance was be set at p<0.05 Results: The prevalence of caries in mandibular first molars in Calicut district was 44.91%. It was also seen the prevalence of caries in mandibular first molars was equal in urban (45%) when compared with rural areas (44.5%). The prevalence of caries on mandibular first molars was more in females (47%)when compared to males (42%)and the results were statistically significant. There is no statistical significance between the prevalence of mandibular first molar caries and habits. In the sample studied, both mandibular left and right mandibular molars were equally affected by caries. It is also seen that the maxillary first molars were less affected when compared to mandibular molars. Conclusion: Since first molars erupts as early as 6 years of age, sufficient dental care must be taken from childhood to improve general oral health and to prevent dental caries. Key Word: Prevalence, Caries, Mandibular, First, Molar ------------------------------------------------------------------------------------------------------------------------------------Date of Submission: 14-05-2020 Date of Acceptance: 29-05-2020 -------------------------------------------------------------------------------------------------------------------------------------
Performance of the Candidate Modulation Wave-forms for 5G Communication Systems

: In surveillance of the contemporary swift growth of progressing networks, attention has been enthralled mostly on the operational properties of multifaceted networks in wireless communications. A number of mammoth simulated networks of this thoughtful have lately been fashioned, which opens an extensive field for the study of their topology, advancement, and the intricate processes which transpire in them. Fifth Generation (5G) will have to survive with a high degree of heterogeneity in relations to services and necessities. Amid these end, the malleable and well-organized use of non-contiguous unexploited spectrum for diverse network disposition scenarios is well-thought-out a key encounter for 5G, New Radio (NR) systems. Furthermore, this study weighs and equates the complexity of the different waveforms. Keen valuations on Spectral Efficiency, Power Spectral Density, Peak-to-Average Power Ratio and robustness to asynchronous multi-user uplink transmission have been explored in this study. The reimbursements of these new wave-form for the anticipated 5G uses cases are noticeably highlighted on illustrative standards and experiments. Each network will be accountable for handling user-mobility, although the terminal will make the final optimal among different wireless/mobile access network suppliers for a given service. The main impact of this study is definition of 5G mobile network perception, which is seen as user-centric notion in its place of operator-centric as in Third Generation (3G) or service-centric concept as seen for Fourth Generation (4G). The 5G terminals will have software distinct radios and modulation scheme as well as new error-control schemes can be downloaded from the Internet on the run. The growth is seen towards the user terminals as an emphasis of the 5G mobile networks. The theoretical results are further verified using Matlab evaluations for Spectral Efficiency, Power Spectral Density and Peak-to-Average ratio. The evaluation results reveal that if an apriori information on the Signal-to-Noise Ratio (SNR) is present, it is possible to highly increase the Spectral Efficiency (SE) of the transmission.
The Role of Gold Prices and Interest Rate in Stock Index: Insights from Vietnam by Using the Autoregressive Distributed Lag Approach

This study aimed to determine the impact of domestic gold prices, interest rates in the stock market index (VNI) in Vietnam for the period of January 2009 to December 2018. This study employed the Autoregressive Distributed Lag (ARDL) to check the association of Independent variable gold prices and the interest rate on the dependent variable stock market index. The results show a close correlation together in the long-run. The Vietnam stock index is adversely affected by fluctuations in the credit market in the short-run. We observed that domestic gold prices and interest rates have one-way causal relations to the stock price index. Similarly, interest rates were causal for gold prices and still not yet had any particular direction. The adjustment in the short-run moves the long-run equilibrium, although the change is quite slow.
Diffusible factors involved in early interactions of actinorhizal symbiosis are modulated by the host plant but are not enough to break the host range barrier.

Nodulation kinetics were analysed in two nitrogen-fixing actinorhizal symbioses that show different pathways for infection: Alnus acuminata H. B. K. , which is infected by Frankia ArI3, and Discaria trinervis (Hooker et Arnot) Reiche, which is infected by Frankia BCU110501. Both pairs are incompatible in cross-inoculation experiments. The dose-response effects in nodulation were studied in A. acuminata seedlings using different concentrations of compatible and incompatible bacteria in co-inoculation experiments. Restriction fragment length polymorphism PCR analysis and plant-trapping analysis showed no co-occupation in A. acuminata nodules when plants were co-inoculated with Frankia BCU110501 and Frankia ArI3. Despite the lack of co-occupation, the noninfective BCU110501 could modify the nodulation parameters of the non-host A. acuminata when infective ArI3 was present in the inoculum. The results suggest that although BCU110501 was not able to induce nodulation in A. acuminata, its interaction with the plant could induce autoregulation as if some level of infection or partial recognition could be achieved. We explored the possibility that physiological complementation of the heterologous Frankia BCU110501 for nodulation of A. acuminata originated in the homologous Frankia ArI3 in the presence of compatible root exudates. Despite the possibility of full activation between bacteria and the host, there was no co-infection of Frankia BCU110501 in Alnus or of Frankia ArI3 in Discaria either. These negative results suggest a physical recognition barrier in actinorhizal symbiosis that operates after early interactions, involving something other than root exudates and diffusible factors of bacterial or plant origin, regardless of the infection pathway.
In Vitro Evaluation of The Antioxidant and Anti-Skin Aging Properties of Green Algal Sulfated Polysaccharides

Abstract Skin aging is a natural phenomenon witnessed by humans. However various intrinsic and extrinsic factors lead to early skin aging. There have been a variety of approaches to combat skin aging one approach uses antioxidants that are known to fight oxidative stress as well as combat problems of aging. In this study, the antioxidant and anti-skin aging properties of sulfated polysaccharides (SPs) from fresh water microalgae Chlamydomonas reinhardtii (Cr) are evaluated. Sulfated polysaccharides were isolated by hot water extraction method and purified by anion-exchange chromatography. The biochemical composition of the extract showed carbohydrate content of 785.07 mg/g, 324.26 mg/g of sulphate and 393.32 mg/g of uronic acid. These extracts which are enriched with SPs were further used for checking antioxidant and anti-skin aging properties. Cr-SPs showed Superoxide anion scavenging activity of 38-92% at 0.1-2 mg/mL, 51-89% of nitric oxide scavenging ability at 0.2-2 mg/mL, 10-58% of hydrogen peroxide scavenging ability at 1- 10 mg/mL, and 28-68% of ferric ion reducing potential at 0.5-5 mg/mL respectively. Furthermore, Cr-SPs showed 90% anti-elastase enzyme activity at 1 mg/mL, 83% and 89% anti-collagenase and anti-hyaluronidase activities at 1 mg/mL and 2 mg/mL respectively. These promising antioxidant and anti- skin aging properties of Cr-SPs pave way to explore the potential of Cr-SPs in cosmeceutic and pharmaceutical formulations as anti-skin aging agents in a cost-effective manner.
Effects of Rearing Density and Raceway Conformation on Growth, Food Conversion, and Survival of Juvenile Spring Chinook Salmon

Abstract Four brood years of juvenile spring chinook salmon Oncorhynchus tshawytscha were reared in conventional and baffled raceways at various rearing densities and loads at Willamette Hatchery, Oregon. A period of rapid linear growth occurred from August to November, but there was little or no growth from November to March when the fish were released. Both fall and winter growth rates were inversely related to rearing density. Final weight and length were also inversely related to rearing density. No significant relationship between load and any growth variable was observed. Fish reared at lower densities in conventional raceways tended to develop bimodal length distributions in winter and early spring. Fish reared in conventional raceways showed significantly larger growth rates and final lengths and weights than those reared in baffled raceways. Food conversions and average delivery times for feed were significantly greater in baffled than in conventional raceways. No significant relationships were o.
Phylogenetic analysis of Alternaria spp. associated with apple core rot and citrus black rot in South Africa

Dry core rot of apple (DCR) and Alternaria black rot of citrus (ABR) have in the past respectively been ascribed to Alternaria alternata and A. citri. In recent years, however, it has been speculated that several other species of Alternaria could also be associated with these diseases. In an attempt to elucidate the identity of these taxa, 25 isolates associated with DCR, and 26 isolates associated with ABR were selected for molecular characterisation. Nucleotide sequences of 1116 sites including the histone gene section and the internal transcribed spacers (ITS 1 and 2) of the rRNA gene were determined for these isolates. The gene trees generated from the individual and combined data sets using maximum parsimony, maximum likelihood and neighbour-joining analysis methods distinguished five clades with strong bootstrap support, namely Alternaria sp. , A. arborescens, A. infectoria, A. tenuissima, and a clade containing isolates variable in morphology, referred to as the Alternaria group. In the alignment of the combined ITS and histone data set, unique transition/transversion substitutions, as well as positional insertions and deletions were observed for each of the above clades. In addition, key sequences in the form of serial composing nucleotides in both the ITS and histone sections of the alignment were also discovered for the molecular identification of A. arborescens, A. infectoria and A. tenuissima. The final phylogeny also indicated that no host specificity existed among the species associated with these two post-harvest disease complexes. Contrary to the host specificity observed on leaf diseases of these hosts in the field, it appears that the post-harvest diseases are the result of adverse storage conditions and opportunism of different small-spored Alternaria spp.