Miracle-dz/UniFAR-FTUs huggingface dataset:
In-capillary sample processing coupled to label-free capillary electrophoresis-mass spectrometry to decipher the native N-glycome of single mammalian cells and ng-level blood isolates

The development of reliable single-cell dispensers and substantial sensitivity improvement in mass spectrometry made proteomic profiling of individual cells achievable. Yet, there are no established methods for single-cell glycome analysis due to the inability to amplify glycans and sample losses associated with sample processing and glycan labeling. In this work, we developed an integrated platform coupling online in-capillary sample processing with high-sensitivity label-free capillary electrophoresis-mass spectrometry for N-glycan profiling of single mammalian cells. Direct and unbiased characterization and quantification of single-cell surface N-glycomes were demonstrated for HeLa and U87 cells, with the detection of up to 100 N-glycans per single cell. Interestingly, N-glycome alterations were unequivocally detected at the single-cell level in HeLa and U87 cells stimulated with lipopolysaccharide. The developed workflow was also applied to the profiling of ng-level amounts of blood-derived protein, extracellular vesicle, and total plasma isolates, resulting in over 170, 220, and 370 quantitated N-glycans, respectively.
Early maladaptive schemas and the risk of nonsuicidal self-injury in college students: A retrospective study

Early maladaptive schemas (EMSs) may be closely related to nonsuicidal self-injury (NSSI). The present study aimed to discuss the relationship between EMS and a personal history of NSSI. This was a retrospective study. A total of 1339 Chinese college students between 16 and 29 years old were asked to complete a questionnaire survey regarding their personal history of NSSI and EMSs. 116 college students reported a history of NSSI (NSSI group), who differed significantly in terms of all EMS-related scores than non-NSSI group(n = 1223). Logistic regression analysis showed that the scores in the disconnection/rejection schema domain exhibited by subjects who were the only child in their family could help differentiate between college students with or without NSSI. The emotional deprivation schema was significantly associated with the lifetime frequency of NSSI behaviors; the vulnerability to harm or illness schema was significantly associated with the internal emotion regulation function and pain associated with NSSI; and the enmeshment/underdeveloped self schema was significantly associated with the addictive features of NSSI. The self-sacrifice schema was significantly associated with the external emotion regulation function of NSSI; the enmeshment/underdeveloped self schema was positively related with the sensation-seeking function of NSSI; and the abandonment/instability schema was negatively related the sensation-seeking function of NSSI. The disconnection/rejection schema domain was highly related with NSSI behaviors. EMS is significantly associated with the history, functions, addictive features, and severity of NSSI. Every EMS is worthy of further investigation and discussion with patients in the context of NSSI behaviors during clinical therapy.
Activity of novel β-lactam/β-lactamase inhibitor combinations against serine carbapenemase-producing carbapenem-resistant Pseudomonas aeruginosa

Abstract Background Antimicrobial resistance in Pseudomonas aeruginosa is complex and multifaceted. While the novel β-lactamase inhibitors (BLIs) avibactam, relebactam and vaborbactam inhibit serine-based β-lactamases, the comparative potency of the novel β-lactam (BL)/BLI combinations against serine carbapenemase-producing P. aeruginosa is unknown. Objectives To compare the in vitro activity of ceftazidime/avibactam, ceftazidime, imipenem/relebactam, imipenem, meropenem/vaborbactam and meropenem against serine β-lactamase-producing P. aeruginosa. Methods Carbapenem-resistant P. aeruginosa were collated through the Enhancing Rational Antimicrobials against Carbapenem-resistant P. aeruginosa (ERACE-PA) Global Surveillance. Isolates positive for serine-based carbapenemases were assessed. MICs were determined by broth microdilution to each novel BL/BLI and BL alone. Results GES was the most common carbapenemase identified (n = 59) followed by KPC (n = 8). Ceftazidime/avibactam had MIC50/MIC90 values of 4/8 mg/L and 91% of isolates were susceptible. Conversely, ceftazidime alone was active against only 3% of isolates. The MIC50/MIC90 of imipenem/relebactam were 16/>16 mg/L and 13% of all isolates were defined as susceptible. Of the KPC-producing isolates, 38% were susceptible to imipenem/relebactam, compared with 0% to imipenem. The meropenem/vaborbactam MIC50/MIC90 were >16/>16 mg/L, and 6% of isolates were susceptible, which was similar to meropenem alone (MIC50/90, >8/>8 mg/L; 3% susceptible) suggesting the addition of vaborbactam cannot overcome co-expressed, non-enzymatic resistance mechanisms. Conclusions Among the novel BL/BLIs, ceftazidime/avibactam displayed better in vitro activity and thus is a rational treatment option for serine carbapenemase-harbouring P. aeruginosa. While imipenem/relebactam displayed some activity, particularly against isolates with blaKPC, meropenem/vaborbactam exhibited poor activity, with MICs similar to meropenem alone.
The prognostic value of tumor-stroma ratio and a newly developed computer-aided quantitative analysis of routine H&E slides in high-grade serous ovarian cancer

Introduction: Tumor-stroma ratio (TSR) is prognostic in multiple cancers, while its role in high-grade serous ovarian cancer (HGSOC) remains unclear. Despite the prognostic insight gained from genetic profiles and tumor-infiltrating lymphocytes (TILs), the prognostic use of histology slides remains limited, while it enables the identification of tumor characteristics via computational pathology reducing scoring time and costs. To address this, this study aimed to assess TSR’s prognostic role in HGSOC and its association with TILs. We additionally developed an algorithm, Ovarian-TSR (OTSR), using deep learning for TSR scoring, comparing it to manual scoring. Methods: 340 patients with advanced-stage who underwent primary debulking surgery (PDS) or neo-adjuvant chemotherapy (NACT) with interval debulking (IDS). TSR was assessed in both the most invasive (MI) and whole tumor (WT) regions through manual scoring by pathologists and quantification using OTSR. Patients were categorized as stroma-rich (≥ 50% stroma) or stroma-poor (< 50%). TILs were evaluated via immunohistochemical staining. Results: In PDS, stroma-rich tumors were significantly associated with a more frequent papillary growth pattern (60% vs 34%), while In NACT stroma-rich tumors had a lower Tumor Regression Grading (TRG 4&5, 21% vs 57%) and increased pleural metastasis (25% vs 16%). Stroma-rich patients had significantly shorter overall and progression-free survival compared to stroma-poor (31 versus 45 months; P < 0.0001, and 15 versus 17 months; P = 0.0008, respectively). Combining stromal percentage and TILs led to three distinct survival groups with good (stroma-poor, high TIL), medium (stroma-rich, high TIL, or; stroma-poor, Low TIL), and poor(stroma-rich, low TIL) survival. These survival groups remained significant in CD8 and CD103 in multivariable analysis (Hazard ratio (HR) = 1.42, 95% Confidence-interval (CI) = 1.02–1.99; HR = 1.49, 95% CI = 1.01–2.18, and HR = 1.48, 95% CI = 1.05–2.08; HR = 2.24, 95% CI = 1.55–3.23, respectively). OTSR was able to recapitulate these results and demonstrated high concordance with expert pathologists (correlation = 0.83). Conclusions: TSR is an independent prognostic factor for survival assessment in HGSOC. Stroma-rich tumors have a worse prognosis and, in the case of NACT, a higher likelihood of pleural metastasis. OTSR provides a cost and time-efficient way of determining TSR with high reproducibility and reduced inter-observer variability.
Effect of fertility and sulphur levels on quality parameter of summer clusterbean (Cyamopsis tetragonoloba L.) under south saurastra region

Clusterbean or guar (Cyamopsis tetragonoloba L.) is a widely grown crop but not on commercial basis on large scale at farmer s field. In the recent years, this crop has assumed great significance due to the presence of a good quality of gum in the endosperm of its seed. Due to diversified uses of clusterbean gum in textile, paper, explosive and mining industries, pharmaceuticals, cosmetic goods and food stuffs, it has ever increasing demand in the international market. It is principally used as a feed for livestock and poultry. India leads among the major guar producing countries of the world, contributing around 75 to 80% to the world s total production (7.5 to 10 lakhs tonnes). (Agril. Statistics at a glance, 2012). According to Aykroyd (1963) the composition of clusterbean is 81.0 g moisture, 10.8 g carbohydrate, 23% protein, ISSN: 2319-7706 Volume 3 Number 9 (2014) pp. 330-334 http://www.ijcmas. com
Growth and yield response of four chickpea cultivars to phosphorus fertilizer rates

The response of grain legumes to phosphorus (P) fertilizer application varies with genotype, environment and management practices. This study aimed at investigating the effects of P fertilizer rates (0, 45 and 90 kg P ha -1 ) on the growth and yield of four chickpea cultivars, two kabuli and two desi types, in the dry environments of Limpopo Province, South Africa. Two field experiments were undertaken in winter 2009 and summer 2009/ 2010 seasons, using a factorial arrangement of chickpea cultivars and P fertilizer rates in a randomized complete block design replicated three times. Crop biomass was determined at 50% flowering and at harvest maturity, and number of pods per plant, seeds per pod, 100 seed weight (100-SW) and grain yield were determined at maturity. Grain yield increased with P fertilizer rates only in the summer sowing. The increase in grain yield with P fertilizer application was associated with greater crop biomass and 100-SW at 45 and 90 kg P ha -1 compared with the control. In contrast, the effect of cultivar on grain yield was significant in both the winter and summer sowings. The variation in grain yield with cultivar was attributed to a similar variation in number of pods per plant (winter sowing) and 100-SW (summer sowing). The lack of response of grain yield to P fertilizer application in the winter sowing was attributed to inadequate moisture received during the crop growth period. Thus, application of P fertilizer may increase grain yield and hence productivity of chickpea at the site of the current study if water is not limiting.
Značaj faze organogeneze formiranje klasića u biologiji prinosa ozimog dvoredog ječma

Izvod: Biljke prolaze kroz tacno defi nisane faze razvica koje mogu biti predstavljene na osnovu promena koje se desavaju na fenotipu biljke ili na osnovu promena u morfologiji apikalnog meristema. Diferencijacija apikalnog meristema odvija se kroz faze koje se nalaze u određenom hronoloskom redosledu. U radu je analiziran znacaj duine formiranja primordija klasica, faza dvostrukog grebena (DR) i njene genotipske i fenotipske povezanosti sa ostalim fazama razvica i komponentama prinosa. Tokom pet sezona (od 2002-2003. do 2006-2007) proucavano je razvice apikalnog meristema kod tri sorte ozimog dvoredog jecma Novosadski 525, Sonate i Monaco. DR faza je bila najkraca u sezonama kada su vladali povoljni vremenski uslovi tokom odvijanja ove faze, a najdua kada su uslovi bili nepovoljni, pre svega visoke temperature. Najkraci DR period imala je ranozrela sorta Novosadski 525, a najdui srednje rana sorta Sonate. Utvrđene su slicne genotipske i fenotipske korelacije između duine DR i duine trajanja ostalih faza razvica i komponenti prinosa. Duina DR perioda bila je u pozitivnoj korelaciji sa duinom perioda formiranja primordija listova, a u negativnoj korelaciji sa ostalim fazama razvica, periodom nalivanja zrna, brojem listova i klasova i etvenim indeksom. Formiranje primordija listova i klasica kao i razvice cvetova i klasica nalazi se pod najvecom kontrolom genotipa, gde je ucesce varijanse sorte preko 80% i godine do 10%. Dobijeni rezultati mogu imati praktican znacaj u oplemenjivanju ozimog dvoredog jecma. Za potvrđivanje ovih rezultata neophodna su detaljnija istraivanja sa vecim brojem sorti. Kljucne reci: faza dvostrukog grebena, genotipske i fenotipske korelacije, jecam (Hordeum vulgare L.), organogeneza
WHAT IS INSIDE THE PITS? MICRO- AND MACROFAUNAL INVESTIGATIONS AT CHALCOLITHIC AND EARLY BRONZE AGE OVÇULAR TEPESI (NAKHCHIVAN, AZERBAIJAN)

The site of Ovcular Tepesi is located in the southern Caucasus (Nakhchivan Autonomous Republic). Two main archaeological phases were recognised at the settlement, namely Late Chalcolithic and Early Bronze Age. More than 1160 litres of soil samples from pits and circular stone structures belonging to the different occupation layers were processed by flotation and analysed. This paper focuses on the zoological finds from these samples, in particular the remains of fish, large mammals and micromammals, in order to determine the function of the pits and structures. Moreover, the material gives an insight into the animal economy, hunting and fishing practices of the inhabitants, as well as the local environment. Thus far, it could not be established whether the differences detected in the species composition between the phases are related to chronology alone or if other factors, such as sample sizes and type of deposits, also played a role. Fish bones were very frequent at the site with cyprinids dominating. Among the large mammals, domestic animals were the most frequent while hunted species were rare. Sheep and goat herding seems to have been the principal subsistence strategy at this site. The small mammal community is composed of synanthropic taxa and, as a consequence, the species diversity was very low (six taxa). House mouse was the most abundant in each feature on the site. *
Cryptomeria Fortune Tree-ring Width Index with Responses to Climate Change in the Southwestern of Sichuan

【Objective】 Dendroclirnatological approach has important significance in global climatic change and is regarded as a very good reference to climate factors.The aim of this study was to examine the relationship between tree-ring width index of Cryptomeria fortune and climatic factors. 【Method】 ARIMA model was employed to estimate the radial growth of C.fortune. Moreover,the tree-ring width index chronology of C.fortune in SW Sichuan was established.Multiple correspondence analysis(MCA) was used to analyze the relationship between radial growth and climates. 【Results】 The results showed a separation phenomenon between tree-ring width index and temperature change but tree-ring index increased with precipitation.The tree-ring width index of C.fortune was correlated significantly with the mean monthly temperature of April and August(P0.05). Similar relationships were found in precipitation of last growing season,especially for precipitation in July(P0.05). Nevertheless,tree-ring width index was negetively correlated with accumulative total sunshine time of current June(P0.05) and October of last growing season(P0.05). The temperature of current growing season(from June to August) was beneficial to increase tree-ring width.However,when the temperature and precipitation of growing season(from May to September) exceeded the threshold,they were conducive to the increase of tree-ring width. 【Conclusion】 Temperature,total precipitation are related closely to tree-ring width index of C.fortune. Additionaly,radial growth is significantly affected by temperature from June to August and total precipitation from May to September.
Ipsilateral femoral neck, intertrochanteric and acetabular fractures with posterior dislocation of the hip: A case report and literature review

Introduction: Traumatic hip dislocation with ipsilateral femoral neck fracture, intertrochanteric fracture and acetabular fracture is a rare and serious injury that occurs mostly in young patients and after high-energy trauma. The treatment of these injuries is a major challenge for orthopedic surgeons; it can have devastating consequences if not treated properly, including avascular necrosis of the femoral head and traumatic osteoarthritis of the hip. In previous case reports only internal fixation of femoral neck fracture was performed without revascularisation and there was a lack of long-term follow-up results. In this report, we fixed the fracture revascularised the patient, and obtained good prognostic results at up to 20 months of follow-up. Case presentation: Here, we report an 18-year-old patient with systemic polytrauma resulting from a car accident. The trauma included ipsilateral posterior hip dislocation, acetabular fracture, femoral intertrochanteric fracture, and femoral neck fracture. In addition, the patient also had an ipsilateral open tibiofibula fracture. We chose the surgical treatment of a proximal femoral locking compression plate (PFLCP), femoral quadratus bone flap graft, and cannulated compression screw to treat the proximal femoral fracture. The patient was followed up for nearly 20 months; the range of motion of his hip was close to normal, and his hip function was good. Discussion and Conclusions: Traumatic hip dislocation with ipsilateral femoral neck fracture, intertrochanteric fracture and acetabular fracture is a rare and serious injury, and surgical intervention is often needed. Because of the high risk of femoral head necrosis in such complex injuries, it is very important to protect/restore the blood supply of the femoral head during surgery. Therefore, in younger patients, we believe that the use of a quadratus femoris bone flap graft and PFLCP is an acceptable treatment for this severe injury. We discuss the management of this rare case and review the current literature to provide the best evidence-based recommendations for this injury pattern. We conclude that for patients with complex ipsilateral proximal femoral and acetabular fractures combined with hip dislocation, the key to surgical treatment, in addition to anatomic reduction and good fixation, is the primary reconstruction of the femoral head blood supply.
Effect of valve opening on starting performance of pump as turbine

The study on transient characteristics of pump as turbines during atypical startup has not been deeply explored yet. In order to reveal the transient characteristics of a small centrifugal pump reversing as turbine during startup process in this paper, the transient hydraulic performance experiments are conducted for three steady rotational speed cases. Under the condition of each case, three, five and, three valve opening scenarios are completed to measure the performance. The dimensionless analysis are also employed so as to better reveal transient behavior of the pump as turbine during atypical startup. The results show that the rise rate of each performance parameter is different, wherein the shaft power and rotational speed have the fastest rising rate, followed by the flow rate, and the head rise is the slowest. It is clearly seen that the shock phenomenon in static pressure easily occurs at the outlet of pump as turbine. With the increase of valve opening, the dimensionless flow rate, head and, power coefficient all show the evolution trend of gradually increasing.
Factors Influencing Delay in Seeking Care for Mental Illness Among a Sample of Adult Saudi Arabian Patients

Background: Treatment and prognosis of psychiatric disorders could be negatively affected by delay in seeking mental health care. The development of mental health services depends on understanding the reasons for delays in help-seeking and pathways to care and the duration of illness before treatment is initiated. Objectives: The aim of the present study is to examine the reasons why patients with psychiatric symptoms delay their visits to psychiatry clinics in Saudi Arabia. Methods: This was a cross-sectional, observational, survey-based study that included 268 patients, of which 60.8% were males. Data were collected through a questionnaire (either online or distributed to patients who attend the outpatient psychiatric clinics) in Taif, Saudi Arabia. Results: Most patients were married males below 40 years old, with a university degree level, employed, and having average financial status. The most common symptom reported by responders was anxiety (41.8%). The most common reason in both age groups was the fear of side effects of medication (31.9% in those over 40 years and 18.4% below 40 years), followed by the patient belief that these are non-psychiatric symptoms and will disappear with time. The trial of folk medicine was the least common reason for both age groups (1.8% in patients below 40 years and 0% for patients above 40 years). Conclusion: Fear of psychotropic drug adverse effects was the most frequent reason given for postponing mental health counseling. This could be due to some unpleasant or intolerable effects. Anxiety was the most common psychiatric symptom among patients delaying their first psychiatric consultation. These findings serve as a guide for the improvement of mental health services and psychoeducation in Saudi Arabia.
Molecular signatures of alternative reproductive strategies in a facultatively social hover wasp

Social insect reproductives and non‐reproductives represent ideal models with which to understand the expression and regulation of alternative phenotypes. Most research in this area has focused on the developmental regulation of reproductive phenotypes in obligately social taxa such as honey bees, while relatively few studies have addressed the molecular correlates of reproductive differentiation in species in which the division of reproductive labour is established only in plastic dominance hierarchies. To address this knowledge gap, we generate the first genome for any stenogastrine wasp and analyse brain transcriptomic data for non‐reproductives and reproductives of the facultatively social species Liostenogaster flavolineata, a representative of one of the simplest forms of social living. By experimentally manipulating the reproductive ‘queues’ exhibited by social colonies of this species, we show that reproductive division of labour in this species is associated with transcriptomic signatures that are more subtle and variable than those observed in social taxa in which colony living has become obligate; that variation in gene expression among non‐reproductives reflects their investment into foraging effort more than their social rank; and that genes associated with reproductive division of labour overlap to some extent with those underlying division of labour in the separate polistine origin of wasp sociality but only explain a small portion of overall variation in this trait. These results indicate that broad patterns of within‐colony transcriptomic differentiation in this species are similar to those in Polistinae but offer little support for the existence of a strongly conserved ‘toolkit’ for sociality.